LOCUS       DASTDG010000001         6223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_821239, whole genome shotgun sequence.
ACCESSION   DASTDG010000001 DASTDG010000000
VERSION     DASTDG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821239"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFO30_00005"
     CDS             complement(<1..659)
                     /locus_tag="VFO30_00005"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET9417697.1"
                     /translation="MRELLLCPPDYYGIEYEINPWMSRARGAEAALAQKQWQDLYATL
                     SKLDCKIHLATPQPRLPDMVFTANAGLTVGRKFIPSNFRHKERAGEAPHFARWMEDHG
                     YEIAWLPPDHYFEGEGDALFGGDALFCGYKFRSDINSHRAVADMLGCLVISVELVDPR
                     FYHLDTCFCPLPDGGAVWFPAAFDEYGQRAIRDHVPELIDVLPEEAMHFSCNAVVLER
                     D"
     gene            complement(791..2038)
                     /locus_tag="VFO30_00010"
     CDS             complement(791..2038)
                     /locus_tag="VFO30_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET9417698.1"
                     /translation="MFSETIELRGHIIDSLILPKVLDQILTRGATFKIAEIKIGQNRS
                     DQSFARIEVSAKERDGLDELVLRLRQHGAQVVERANVQLASAPADGVFPDDFYVTTNQ
                     QTFVRVDDQQIEVQPAMSDSAIAVDRKAHTARAMKFFDVRQGDEIVVGQQGVRVVPLQ
                     RATSHTDVFQFINTSVAADEPKSAVIRELARELVRAREAGGHIALVAGPALVLTGAGE
                     HLQRLIERGYIDRLFAGNAFAVADIERALFGTSLGTRLDSAIAGAGHENHLRAINTIR
                     RCGGLAAAVEKKILTSGIMHACVQRKIDIVLTGSIRDQGPIPGVVTDVVEAQKIMRDK
                     LVDVTHVLLMGTVQHALAIAAMLAPTVKTICVDINEPAVARIIERQPFQTVGLVTDLE
                     PFLRELADCVDELATTVTKTRSS"
     gene            2128..2304
                     /locus_tag="VFO30_00015"
     CDS             2128..2304
                     /locus_tag="VFO30_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417699.1"
                     /translation="MPQHPVKKPNVRGPSPSRKAHLALREKLNRKFKYPPALPRKSFD
                     KARAFDRNELKQHE"
     gene            2297..2521
                     /locus_tag="VFO30_00020"
     CDS             2297..2521
                     /locus_tag="VFO30_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417700.1"
                     /translation="MSDEGWAAVREKIAATLANKPQIFITHPAELKVLRRMSDEQVRS
                     FAAEHGWRVVRRLGGKQIEFYNDAGARTQL"
     gene            complement(2607..3452)
                     /locus_tag="VFO30_00025"
     CDS             complement(2607..3452)
                     /locus_tag="VFO30_00025"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET9417701.1"
                     /translation="MKDHALEAHATSPYLRAMSDKPSKLKIADRVFTSRLLVGTGKFA
                     SNELMRDALIASGTELVTVALRRADLSGKHDAFANILDFIDPKRFLLLPNTSGARNAA
                     EAVRLARLAVAAGLPSWVKLEIHPDPRYLLPDPVETLAAAETLVKEGFTVLPYINADP
                     VLAKRLQDVGTATVMPLGSPIGSNRGIETRAQIEIIIEQATVPVVVDAGLGAPSHAAQ
                     AMEMGADAVLVNTAIAIAPDPVRMAQAFRKAIEAGREAREIGLAEKLQTASATSPLTA
                     LFSKS"
     gene            3521..4363
                     /locus_tag="VFO30_00030"
     CDS             3521..4363
                     /locus_tag="VFO30_00030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9417702.1"
                     /translation="MTEFDLRLEDCITGLARLPREHVDLVVTSPPYNLGVRYGKFSDR
                     QDRQSYLRWCSEWAAEIRRVLKPTGSFFLNVGAAPSNPMLPHEIVLQFRDLFVLQNTI
                     HWIKSIAIDHPHLKPLPEGEEDAKRPVRANTTTFGHFKPISSPRFLNDCHEYVFHFSK
                     TGKIDIDRLALGVPYQDKSNISRWSHARGRDKRCRGNTWYIPYETIQSRAKERPHPAT
                     FPVRLAEWCIKLHGVSRVKIMLDPFLGIGNSAVAATKCGMRRFIGFEIDETYLAEAKR
                     RLKL"
     gene            complement(4397..5458)
                     /locus_tag="VFO30_00035"
     CDS             complement(4397..5458)
                     /locus_tag="VFO30_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9417703.1"
                     /translation="MQLLTLTEIRRSAQSGPVTARVHVQVEGAAPRLTREQQPYCELN
                     LADACDRMTLRVWSDHPAYKACSSLTDSDFIELTGEFHQHSQYGLEARKWTVRPLTEQ
                     EKKELLQGPPELRARQAADWDYVVKTLAAIADPRLRALSEAFLKEWGERFRRTAAARN
                     YHHARRGGLVEHTAQMMRVVREIAPLYPQLNLDLLLAGILFHDCGKLWENALPENGFV
                     MNYDERGELMGHISIGLELVNSLWRKMSAANDEAWKNLTPPSEDVRLHLLHLIGAHHG
                     EPQFGSPVSPKTPEAMALHYIDNLDARLEMFAAGYTVAKPLAARIFDRVRPLPGNLVK
                     SLDKFQQQTNTGRSDGTLL"
     gene            complement(5505..6011)
                     /locus_tag="VFO30_00040"
     CDS             complement(5505..6011)
                     /locus_tag="VFO30_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET9417704.1"
                     /translation="MKTIICIGALCAFTAFAHAQDVTRATATLEPKSGSKVTGTVAFS
                     KTGAETRVVADIENLTPGKHGFHIHEKGDCSAADASSAGGHFNPTHQHHGGPAGTERH
                     AGDLGNIEADASGKAHLDWTGKLSFSGSDSIIGKSVVVHEKEDDLKTDPAGNSGARVA
                     CGEIHAAQ"
BASE COUNT         1266 a         1900 c         1800 g         1257 t
ORIGIN      
        1 atatcgcgct cgagcacgac cgcgttgcaa gaaaaatgca tagcctcctc tggaaggacg
       61 tcgatcaact ccggcacgtg gtcgcgaatg gcgcgttgtc catactcatc gaacgccgcc
      121 ggaaaccata cggcgccacc atcgggcagc ggacaaaagc aggtgtcgag gtggtaaaag
      181 cgcgggtcga caagctccac tgaaatgaca aggcagccga gcatgtcggc aacggcgcga
      241 tgcgagttga tgtcggagcg aaatttgtag ccgcaaaaaa gcgcgtcacc gccgaagagc
      301 gcatcgcctt caccttcgaa ataatggtcc ggcggcaacc acgcgatttc atatccatga
      361 tcctccatcc agcgcgcgaa gtgcggcgct tcgccggcgc gctccttgtg ccgaaaattg
      421 ctcgggatga attttcttcc gacggtaaga ccggcgttcg cggtgaacac catgtcgggc
      481 aaacgtggtt gcggcgtagc caggtgaatc ttgcagtcga gctttgataa ggtggcgtac
      541 agatcctgcc actgcttttg cgccagcgcc gcctccgcgc cgcgcgcgcg actcatccag
      601 ggattgatct cgtattcgat gccgtagtaa tccggcgggc aaagcaacag ttcgcgcata
      661 agatcgacaa tctacttgtt ctcggattgc gtcgcgcgca agccacggac aacgccttcg
      721 agtgaccctc acctggcggt gacgcgcgtc ctcctctccc gtgtcacgac tgggcggagt
      781 gttgagagaa ttaagatgag cgtgttttgg tcaccgtcgt cgcaagttcg tcaacgcaat
      841 ccgccagctc gcgcagaaaa ggttcgaggt cggtcactag tccgacggtt tggaacggct
      901 ggcgctcgat aatccgcgcg acggccggct cgttgatatc gacgcagatt gttttcaccg
      961 tcggtgcgag catcgccgcg atggccagcg catgctgcac cgtgcccatg agcagcacgt
     1021 gcgtcacgtc aacgagtttg tcgcgcatga tcttttgcgc ttcgacgaca tccgtcacca
     1081 cgcccggaat tggcccctgg tcacggattg atccggtgag cacgatgtcg atcttgcgtt
     1141 gcacgcaggc gtgcatgatc cccgaggtaa gaattttctt ttccaccgcc gcggccaggc
     1201 cgccacagcg ccggatcgta ttgatcgcgc gtaagtgatt ttcgtgtccg gcaccagcga
     1261 tcgcgctgtc aagtcgcgtg cctaacgaag tgccaaacaa cgcgcgctcg atgtcggcaa
     1321 cagcaaacgc attgccggcg aaaagccggt cgatatagcc gcgctcgatg agccgctgca
     1381 aatgttcacc cgcgccggtc aacaccagcg ccgggccggc cacgagcgcg atgtggccac
     1441 ctgcttcgcg cgcacgcacc aactcgcgtg ccagttcgcg aatcaccgcc gacttcggct
     1501 catcggcggc gacactcgta ttgatgaact ggaagacgtc cgtgtgcgag gtcgcgcgct
     1561 gcagcggaac gactcgcaca ccttgctggc cgaccacgat ttcgtcgcct tggcgaacat
     1621 caaaaaattt catcgcgcgc gcggtatgcg ctttccggtc aacagcgatt gcgctgtcac
     1681 tcattgccgg ctgcacttcg atttgttgat cgtcgacgcg gacaaacgtc tgctgattgg
     1741 tggtgacgta aaagtcatca gggaaaacgc cgtccgctgg tgcgctggcg agttgcacat
     1801 tggcgcgctc caccacttgc gcgccgtgtt ggcgcagccg cagtaccaat tcgtcaaggc
     1861 cgtcccgttc tttcgccgag acttcgatcc gcgcgaagct ctgatcggag cggttctggc
     1921 caatcttgat ctcggcgatc ttaaatgttg cgccgcgggt aaggatttgg tccaacacct
     1981 tcggcaaaat cagcgaatcg atgatgtgac cgcgtagctc gatcgtttcg gaaaacatcg
     2041 gcaaacgtta gccaatcaac gcgcccgcgc caattgcgtc ggcacgtagc atcggcatct
     2101 tgccgatgaa acatgggctg gaaggccatg ccacaacacc ctgtgaagaa accaaacgtc
     2161 cgtggaccat caccgagccg caaagcgcat ctcgcgctgc gtgagaaact gaatcgcaaa
     2221 ttcaaatacc cgccggcttt gccgcgaaaa tcattcgaca aggcgcgcgc gttcgatcgc
     2281 aacgagctca agcagcatga gtgacgaggg ttgggccgcg gtgagggaga aaatcgcggc
     2341 gacgctcgcg aacaaaccgc agattttcat cacgcatccc gccgagctga aggtgttgcg
     2401 gcgcatgagc gacgaacaag tgcgcagttt tgccgcggaa cacggctggc gggtggtccg
     2461 ccggcttggc ggcaaacaaa tcgagttcta caacgacgcc ggcgcccgca cacaactgta
     2521 gcttggattc tgtagccacg cgcctgcggc gcgtccgaga ttaacggtca aagaacggcc
     2581 cacaggggcc gtggctacag tcgcgcctag ctcttcgaga aaagcgcagt cagcggactc
     2641 gtcgcggacg cagtttgcag tttctccgct aaaccgatct cgcgcgcttc gcgtccagcc
     2701 tcaattgctt tccggaacgc ctgcgccatt cgcacaggat cgggtgcgat cgcgatcgcg
     2761 gtgttcacca gcactgcatc cgcgcccatc tccattgctt gcgcggcgtg gctcggcgcg
     2821 ccgaggcccg cgtcaacgac gaccggcaca gttgcctgct cgatgatgat ctcgatctgc
     2881 gcgcgtgtct cgattccgcg gttgctcccg atcggcgaac cgagcggcat cacggtcgcg
     2941 gtgccgacgt cctgcaggcg tttcgccaac actggatcgg cattgatgta gggcaaaacc
     3001 gtgaaccctt ctttcaccaa agtttccgcc gcagcgagcg tttccaccgg gtccggcagc
     3061 aaatagcgcg ggtcagggtg gatctcgagc ttcacccagc tcggtaaacc agcggcgacc
     3121 gcgaggcgcg ccaatcgcac cgcctcggca gcattccggg cgccgctcgt gttcggcaag
     3181 agcagaaacc gcttcggatc gataaaatcg agaatgttcg cgaatgcatc gtgtttgccg
     3241 gagagatcgg cccgccgcaa cgcgaccgtc acaagctcgg tgccgctcgc gatcaacgca
     3301 tcgcgcatca gctcgttcga cgcgaacttg cccgtgccca cgagcaaacg cgacgtgaac
     3361 acgcggtcgg cgatcttcag cttcgacggc ttgtccgaca ttgcccgaag ataaggtgaa
     3421 gtggcatgcg cttccagcgc atgatctttc aattcatcgg caggatgccg atgccacttt
     3481 cttctgtacg aagccaatca cttcgctcac cttgtccgct gtgacggaat ttgatctgcg
     3541 cctcgaagac tgcattacag gattggcgcg gttgccgcgt gaacatgtcg atctcgtcgt
     3601 cacatcgccg ccttacaatc tcggcgtgcg ctacggcaaa ttttctgacc ggcaagatcg
     3661 acaatcctat ttgcgctggt gctccgaatg ggctgcggag atccgccgtg tcctcaaacc
     3721 gacgggctcg ttctttctga acgtcggcgc ggcgccatcg aatccgatgt tgccgcacga
     3781 aatcgtgctg caatttcggg acttattcgt gctgcaaaac actattcact ggatcaagtc
     3841 gatcgcgatc gatcaccctc acctcaagcc tctccctgag ggagaggagg acgcgaagcg
     3901 cccggtgagg gcaaacacaa caaccttcgg tcatttcaaa ccaatcagct caccgcgatt
     3961 cctgaacgat tgtcacgaat acgtttttca tttcagcaaa actggcaaga tcgacatcga
     4021 ccggctcgcg ctcggcgtgc cgtatcagga caaaagcaac atttctcgct ggtcgcacgc
     4081 gcgcggacgc gacaaacgct gccgcggcaa cacctggtat attccgtacg aaacaatcca
     4141 gagccgcgcg aaagagcggc cgcatccggc gacgtttccc gttcggctcg ctgagtggtg
     4201 catcaaactg cacggcgtat cgcgcgtaaa gataatgctg gatccatttc tcggcattgg
     4261 aaactccgcc gtggcggcga cgaaatgcgg catgagaaga tttatcggct tcgaaatcga
     4321 cgagacgtat ctcgccgaag cgaagcgacg cctgaaattg taggggaagc ttcccatggg
     4381 acgctagcag cgtcccctac agtaatgtgc cgtctgaccg gccagtgttt gtctgttgct
     4441 gaaatttgtc gagcgatttg accaaatttc ccggcaacgg acggacgcga tcaaagatgc
     4501 gcgccgcgag cggctttgcg acagtgtagc cggcggcgaa catctccagg cgcgcatcca
     4561 gattgtcgat gtaatgcaac gccatggctt cgggagtctt cggactcacg ggagaaccga
     4621 actgcggttc gccgtgatgc gcgccgatca ggtgcagcaa atgcaaacgc acgtcctctg
     4681 atggcggggt cagatttttc caagcttcgt catttgcggc ggacatcttt cgccagagtg
     4741 aattgacgag ctcgagccca atcgagatgt gccccatcaa ttcgccgcgc tcgtcgtagt
     4801 tcatgacgaa gccgttttcg ggcagcgcgt tctcccagag cttgccgcag tcgtggaaca
     4861 aaatgccggc aagcagaaga tcgagattca actgcggata taacggggcg atctcgcgga
     4921 cgacgcgcat catctgcgcg gtatgctcga ccaggccgcc gcgtcgggcg tgatggtagt
     4981 tgcgcgcggc ggccgtgcgc cgaaaccgtt cgccccattc cttcaaaaat gcttctgaca
     5041 acgcgcgcaa tcgcggatcg gcgattgcgg caagcgtttt cacaacataa tcccagtcgg
     5101 ctgcttgtct ggcgcgcagt tccggcgggc cttgcaaaag ttctttcttc tcctgctcgg
     5161 tcagcggccg caccgtccac ttgcgcgcct cgaggccata ttgcgaatgc tgatgaaatt
     5221 cgcccgtcag ttcgatgaaa tcggaatcgg tcagcgaact gcacgccttg tacgcgggat
     5281 gatcgctcca gactcgcagc gtcattcgat cgcaagcgtc cgcgaggttt agctcacagt
     5341 acggctgttg ctcgcgcgtc agccgcggcg cggcgccttc cacctgtaca tgcacgcgcg
     5401 ccgtcaccgg gccgctttgc gcggatcggc gaatctccgt gagcgtcaaa agttgcatgt
     5461 cgatcttgca gtttcgacat ccgatcgcat gtcgaactat cggcttactg cgccgcgtga
     5521 atttcgccgc aggcgacccg tgcgccggag ttaccggccg gatcggtttt caggtcgtct
     5581 tctttttcgt gcacgaccac cgatttaccg atgattgaat cgctcccact gaagctcagt
     5641 ttgccagtcc aatcgaggtg cgctttgccg gaggcgtccg cttcaatgtt gccgagatcg
     5701 cccgcatgac gctcggtccc ggcggggcca ccgtgatgtt ggtgcgtcgg attaaaatgg
     5761 ccaccggccg acgatgcatc cgccgccgag caatcgcctt tctcgtggat atgaaaaccg
     5821 tgcttgcccg gcgtgaggtt ttctatgtcg gcaacaactc gcgtttcagc gccggttttg
     5881 ctgaatgcga ccgtgccggt gactttactg cctgatttcg gctccagcgt cgctgtcgct
     5941 ctagtgacat cctgcgcgtg cgcgaaagcg gtgaacgcgc acaacgcccc tatgcaaata
     6001 atcgttttca tgagccgacg gtacggcgct ccggcagaaa tgaaaactag gaatcgagca
     6061 ggcgctgctc cagcccggca taggcatcga cccgccggtc gcgcagaaag ggccagtggg
     6121 ttcgatgtcg atcgaccgcg ctccaatcga cctcaccgat cacgatttct tcgcgatcag
     6181 acgagccttt gccgatgagc tcgccatccg ggccgcagat gaa
//
LOCUS       DASTDG010000002         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_167207, whole genome shotgun sequence.
ACCESSION   DASTDG010000002 DASTDG010000000
VERSION     DASTDG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167207"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFO30_00045"
     CDS             <1..734
                     /locus_tag="VFO30_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417705.1"
                     /translation="FVHKSGPIVDVSSRVFPYIDEIVINLYGSEIDALPPRSQGIAGG
                     TKPAFEVAAVNLTARNIGWRHVPLNLQFEARGVCLDQAFDARGEIVLLPRRIDDGEIS
                     FSAAQLDLEEAIARVLATEARAHGITIEQVRLALRERGPRSIAGEVRIQARKFVRAKI
                     DIYGQLEVDENFVATVSQLSCHGAGAIASRACRALEPHLQRFKCRSLPLKSLPLGETQ
                     LRTLRIAVTDAVELRLGFGSAGAQT"
     gene            complement(772..1878)
                     /locus_tag="VFO30_00050"
     CDS             complement(772..1878)
                     /locus_tag="VFO30_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9417706.1"
                     /translation="MKLIDRFTSRELLVNVLFAIAVLSLVLVVGNIFRKLLPLLVNHD
                     VPVEYLISFIAYVLPFSLIFTIPWGLLTAILLVFGRLSADNELIALRANGVSVPRVCL
                     PLAGIAIVCTVICLWLNVQAAPAAQEKLRSTIFDLATRDPMALFGSDQVIDEFPGRKI
                     YVGKKEGNKLENITVFELDRSAMPMKVTYARTGLLEADLPNKRILMHLYNARYQQRDE
                     NNPADLRKVRDGINMAEGTLPISLEELYERQKKRPSRSALSIEQLLDQLKSESSRERS
                     ASRTEINKRFSFPFSCLAFAVIGVPLGVTAHRKETSVGFAMGLIVAVSYFLFVIIGDT
                     LRSNPQVHPELLVWFPNALFLFLGAILFRRLARQ"
     gene            2038..3345
                     /locus_tag="VFO30_00055"
     CDS             2038..3345
                     /locus_tag="VFO30_00055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9417707.1"
                     /translation="MSQIDMYDVAIIGGGPGGSTAAALLARGGRRVVLFEREEFPRFH
                     IGESLLPFSMKAFTRLGLHEKFIRAGFMKKFGGEMFGSCSEKGTKFYFKDAYRSQTDH
                     AYQVTRSDFDKVLLDHAAENGAEVHEQTVVTRVTFANEHVDLAIGQNASSHNVRARYV
                     IDASGRNSLLGNQLGIKKTYDHLQKLSIFAHYDGVWRPDGIDATLTLLIRGIDRWFWV
                     IPLTTERTSIGVVLDKETFRQSAMSAEDFLEHSLAEQPIIAKRMTNAHRASQVHVAAD
                     FSYRSTKLHGDRWLLVGDAAGFIDPIFSSGVFLAVFSGEQAADALTEALDHPRRAKRL
                     FRRYDHAVNRAMDIYLRFVNAWYTKEFIEVFLAPRDVLGLPPAVNAVLGGNIGNSFAI
                     RWRMWVFYFLVWLQKRYPIVPRLTLVPKKTGAPAGVEPAGATW"
     gene            3342..3767
                     /locus_tag="VFO30_00060"
     CDS             3342..3767
                     /locus_tag="VFO30_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417708.1"
                     /translation="MSQITRAIVGALLIVDLAACATTSRHQFAEPAARWQTRNGQLLY
                     RNANTTLIGDAFVRFTKTGDFELTFWKGPGVTLLSLREDMTFAAVKGALAGPGWSGPL
                     TRAPKQLRGWLGLRGAIISAQGRHRVRYASDTETFVLRF"
     gene            3886..>5057
                     /locus_tag="VFO30_00065"
     CDS             3886..>5057
                     /locus_tag="VFO30_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9417709.1"
                     /translation="MRISDLIARIITHRRGWVWCGIAILTLACLTILITRMQLDSDVV
                     NMLPGRFESVQGLKIYDREFEQARELTFALECQPQDVDKLEQFAPAFADKLRQQPWCE
                     RVLAGSPLETPDGIRDLQTIAVPLLLNLEPAAFAQTISMLQPQKIRERLQRFRDQIEA
                     GSPRPQFELGFDPLGLIAPALKPFAESNALEEEQPLASPDRTLRVFLVVTNQPSLSAF
                     ECQRLMREVNAFRAGARDGWDGAPLQVLVTGRAAFVAEISLSMRYDILATLGTSVLLV
                     ALIFFVGFRRWMPLLGMAFSLLLSCLVALALGLLIFGRLAMVAVGFSAILVGLGVDFA
                     ILIFGRYQQARIDGEAYEQAIATSVAKLGRAVFFGALTTAVGFMALILSGALGFAQL"
BASE COUNT          997 a         1492 c         1512 g         1056 t
ORIGIN      
        1 gctttgtcca taaaagcggg ccgattgtcg atgttagctc gcgcgtgttt ccatacatcg
       61 acgagatcgt gatcaacttg tacggctcag agatcgacgc gttgcctccg aggtcgcaag
      121 gtattgcggg cgggacaaag cccgcgttcg aagttgcggc agtcaacctg acggcgcgca
      181 acatcggttg gcgccacgtg ccgttgaatc tgcaattcga agcgcgcggc gtttgcctcg
      241 atcaagcttt cgacgcacgg ggcgagatcg tgttgctacc gcgcagaatt gacgacggtg
      301 agatctcatt ttcagctgcg caacttgatt tggaggaggc gatcgccaga gttcttgcca
      361 cggaggcgcg ggcacacggc atcacgattg agcaggtgcg actagcgctg cgcgagcgcg
      421 gcccgcgctc gatcgcgggt gaagtgagga ttcaagcgcg caagttcgtc cgcgccaaga
      481 tcgacatcta cggccagctc gaggttgacg aaaatttcgt cgctacagtt tcgcagctca
      541 gctgccacgg cgccggcgcg atcgcctcgc gggcgtgtag agcgctcgag ccgcatctgc
      601 agcgcttcaa gtgccgaagc ttgccgctca aatcattgcc gctcggtgaa actcaactgc
      661 gtaccctccg aatcgcggtc accgacgcag ttgagctccg cctcggcttc ggcagcgcgg
      721 gggcgcagac gtaaatgttc gcgatctcct gcggcggatc gaactttgcg tttattgccg
      781 ggccaaccgt cggaacaaga tcgcgccgag gaagagaaac agcgcgtttg ggaaccagac
      841 gagcaattcc ggatggacct gcggattcga gcgaagggtg tcgccgatga tcacgaaaag
      901 aaaatacgag acggcgacga tgagccccat ggcgaagcca accgatgttt ctttgcggtg
      961 cgcggtgaca ccgagcggca cgccgattac cgcaaacgcg aggcacgaga atgggaatga
     1021 aaagcgtttg ttgatctcgg tgcggctggc gctgcgctcg cgcgagcttt cgctcttcaa
     1081 ttggtcgagc aactgctcga tcgataacgc tgaccggctt ggccgtttct tctgccgttc
     1141 gtacagttcc tccaggctga tcggcagcgt tccttcagcc atgttgatgc cgtcgcggac
     1201 cttgcggaga tcggccgggt tgttttcgtc ccgctgctga taacgcgcgt tgtagagatg
     1261 catcaggatg cgtttgttcg gcagatcggc ctcgagcaac ccggtcctgg cgtaagtcac
     1321 cttcatcggc atcgcgctcc ggtcgagctc gaacacggtg atgttctcga gcttgttgcc
     1381 ctcctttttg ccaacgtaaa ttttgcggcc gggaaactcg tcgatcacct gatcgctgcc
     1441 gaagagcgcc attgggtcac gcgtggcgag atcaaaaatc gtgctgcgca atttttcctg
     1501 cgcggccgga gcggcctgca cgttcagcca caggcaaatg actgtgcaaa caatcgcgat
     1561 ccccgccaac ggcaggcaga cccgcggaac gcttacgcca ttagcgcgga gcgcgatcaa
     1621 ctcgttgtcc gccgatagcc ggccaaacac gagcaggatc gcggtcagca gcccccacgg
     1681 aatggtgaaa atgagcgaga acggcaggac ataagcaatg aaactgatga ggtactccac
     1741 cggtacgtcg tggttcacga gcagtggcag gagtttgcga aaaatgttgc cgacgacgag
     1801 cacaaggctc agcaccgcga tcgcaaacag cacgttcacg agcaactcgc ggctggtgaa
     1861 ccgatcgatc aacttcattt gcgttctgtt acgtgtaatg gaacaaggcg caaggtggat
     1921 cgcgatctcc gcgcgcggtg gtggggagtg gcggcgaagc cgcaattagt tagcatcgtc
     1981 cgcggagcga ccaatccacc ttgcttcgct tacgcgtttg taaaaaatta aggcgcagtg
     2041 agccagatcg acatgtacga cgttgccatc atcggcggcg ggccgggcgg tagtacggct
     2101 gctgcgctcc tcgcacgcgg tggtcggcgc gtcgttcttt tcgagcgcga agagtttccg
     2161 cgctttcaca ttggtgagtc gcttttgccg ttcagcatga aggcgttcac ccggcttggc
     2221 ttacacgaga aatttatccg cgccgggttc atgaaaaagt tcggcggcga aatgttcggc
     2281 tcctgctccg agaagggaac caagttctat ttcaaagatg cgtatcgctc ccaaaccgat
     2341 cacgcctacc aggtcacgcg ttcggatttc gacaaggtac tgctcgacca cgccgcggaa
     2401 aatggcgcgg aagtgcatga gcagaccgtg gtgacgcgcg ttacgtttgc gaacgaacat
     2461 gtcgatcttg ccattgggca gaatgcgtcg tcacacaacg tgcgcgctcg ttacgtcatc
     2521 gatgcgagcg gccgcaattc gctgctcggc aatcaactcg gaatcaagaa gacatacgat
     2581 catctccaga agctgtccat cttcgcgcac tacgacggcg tttggcggcc cgatggcatc
     2641 gatgcgacgc tgaccctttt gatacgcggc atcgaccgct ggttttgggt catcccgctc
     2701 acaaccgaac gcaccagcat cggtgtcgtg ctcgacaaag aaacgttccg ccaatcggca
     2761 atgagcgcgg aagatttcct ggaacactca ctggccgaac agccgatcat cgccaagcgc
     2821 atgaccaatg cgcaccgcgc ctcccaagtc catgttgcgg cggatttttc ctaccgcagt
     2881 acgaaacttc acggcgaccg ctggttgctc gttggcgatg ccgctggatt catcgatcca
     2941 attttcagca gcggagtttt tctggccgtg ttcagcggtg aacaggctgc ggatgcgctc
     3001 actgaggcgc tcgatcatcc gcgtcgtgcg aaaagactct tccgccgcta cgaccacgcg
     3061 gtgaaccgcg cgatggacat ttacctccgc ttcgtcaacg cttggtacac caaggaattc
     3121 atcgaggttt ttctcgcgcc acgcgacgta ctcggtttac cgccagccgt caacgcggtg
     3181 ctcggcggca acattggcaa cagcttcgcc attcgctggc gcatgtgggt cttctatttt
     3241 ctggtttggc tacaaaaacg ttatcccatc gtgccgcgac tgacactcgt tccgaagaaa
     3301 acgggagcgc cggccggagt ggagcctgcc ggcgcaacct ggtgagccaa atcacgcgcg
     3361 caatcgtcgg cgcgctgttg attgtcgatc ttgcggcgtg cgcgacaact tcgcggcatc
     3421 aattcgccga gcccgcggcc aggtggcaaa cgcgcaacgg ccagctcctt taccgcaatg
     3481 ccaacacgac cttgatcggc gacgcgtttg tgcgctttac caaaactggc gattttgaac
     3541 tgacattttg gaaaggaccc ggcgtaacac tgctatcgtt acgcgaagac atgacattcg
     3601 ccgcagtgaa aggcgcgctc gctggtccgg gctggtcagg tccgcttacc cgcgcaccga
     3661 aacaactgcg cggttggctg ggattgcgcg gcgcgatcat ttcagcgcaa ggtcgacatc
     3721 gggtacgcta cgcctccgac acggagactt tcgtcttgcg cttttaatct gcggcagaac
     3781 cgttgccctc acccgtcctc tccctgacgg agaggcgaac gggccagcgc cggtgagggc
     3841 tgcacaagac gtgcgttaat ccgtgaacag cgaaattggt tactgatgcg gatcagcgat
     3901 ttgatcgcgc ggattatcac gcaccgccgc ggctgggtct ggtgcggcat cgcgattcta
     3961 accctcgcgt gcctaacaat cctgatcacg cgcatgcagc tcgactccga tgtcgtcaac
     4021 atgctgcccg ggcgctttga atctgtccaa ggactgaaga tctacgatcg cgagtttgaa
     4081 caggcacgtg agctgacttt cgcgctcgaa tgccagccgc aggatgtcga caaactcgag
     4141 caattcgcac ccgcgttcgc cgacaaactg cgccagcaac catggtgcga acgcgttctc
     4201 gccggctcgc ccttggaaac gcccgatggg atccgcgatc tgcaaacaat cgcggtgccg
     4261 ctgctgttaa acctcgaacc ggcggctttc gcgcaaacga tctcgatgtt gcagccgcag
     4321 aagatccgcg aacgtctgca acgcttccgc gatcagatcg aagcgggctc cccgcgtccg
     4381 cagtttgagc tcgggttcga cccactgggg ctgatcgcgc cggcgctaaa accgttcgcc
     4441 gaaagcaacg cgctcgaaga agagcaaccg ctagcctcgc cagatcggac gctgcgcgtg
     4501 tttctcgtcg tgacgaacca gccatcgctc agcgcgtttg agtgccagcg gctcatgcgc
     4561 gaggtgaacg cgtttcgcgc aggcgcacgt gacgggtggg acggcgcgcc gctgcaagtg
     4621 cttgtcaccg gccgcgccgc gtttgtcgcc gaaatttcgc tcagtatgcg ctacgacatc
     4681 ctagcgacgc tcggcacatc ggtattgttg gtcgcgttga tcttctttgt tggatttcgc
     4741 cgctggatgc cgctgctggg gatggcattc tcgcttctcc tctcgtgcct cgtcgcgctc
     4801 gctctcgggc tgttgatctt cggtcggtta gcaatggtgg cggtcgggtt ctcggcaatt
     4861 ctggtcggct tgggggtcga ttttgcgatt cttattttcg gccgctatca acaagcgcgg
     4921 atcgatggcg aggcgtacga acaggcaatc gccacatcgg tcgcgaaact tggccgggcc
     4981 gttttctttg gtgcgctcac gaccgcggtg ggttttatgg cgctgatcct gagcggcgca
     5041 ctcggttttg cgcaact
//
LOCUS       DASTDG010000003         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1004899, whole genome shotgun sequence.
ACCESSION   DASTDG010000003 DASTDG010000000
VERSION     DASTDG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004899"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..737)
                     /locus_tag="VFO30_00070"
     CDS             complement(21..737)
                     /locus_tag="VFO30_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET9417710.1"
                     /translation="MAASTKAVPRHIAIIMDGNGRWARARGLPRIKGHEQGAQAAREC
                     VEGCAELKVEYLTLYAFSAENWQRPKGEVLALMRLLEKFLKEKTPELLEKNVRLQAIG
                     RLTDLPQSCQEQLHKTIEQTAVNNGLTLILALSYGGRLEIIDGIKSLLRAIELGHIDR
                     AMIDVDMFSKHLYTRYYPDPDLLIRTSGEMRLSNFLLWQLSYTEIYVTPRLWPDFTKK
                     DLFAAVDEYGRRQRRYGAVE"
     gene            complement(740..2008)
                     /locus_tag="VFO30_00075"
     CDS             complement(740..2008)
                     /locus_tag="VFO30_00075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9417711.1"
                     /translation="MANTILIGAQWGDEGKGKIIDVLTAKADIVVRSQGGNNAGHTVI
                     HRGIKYVLHLIPSGILRRGKVCVIGNGVVIDPIALVAEMEGLRKRGIKIDHNLLISDC
                     AHLVLPYHRLLDEQRELRKGRAKLGTTKRGIGPAYGDKAARTGLRISDLMQPILFSKK
                     LQAKIVENNSILQALGAHSISFRDVNRSYLTAAEKLRPFIANTVVYLHRALEGNKEIL
                     FEGAQGTFLDIDHGTYPYVTSSNTTAGGACTGTGVPPHRMDRVLGVMKAYTTRVGEGP
                     LPTEDRQFAEMLHKLGREFGATTGRARRCGWFDAVATRYARMINGIDELAITNLDGLD
                     AVDPIRVCVGYRLNEKRLDVPPSDASQLDNCEPIYEQMRGWKQSTHAARKFSQLPRAA
                     KEYVRRISELTGARLSIVSVGPGRRQTIQL"
     gene            2117..2269
                     /locus_tag="VFO30_00080"
     CDS             2117..2269
                     /locus_tag="VFO30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HET9417712.1"
                     /translation="MRTILLIILVLLLIGALPTWPYSTGWGYYPSGGLGLILLIVLIL
                     ALAGRL"
     gene            complement(2266..>3281)
                     /locus_tag="VFO30_00085"
     CDS             complement(2266..>3281)
                     /locus_tag="VFO30_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417713.1"
                     /translation="KLKLDVSAPPMSRLVHSSQSYDLYLHGLFYSDKTTEEALRTSLQ
                     YFERALELDPQFGRAWTGIAKAWLWLADAYVPPREAYPKVRDAATNALKIDNDDAEAH
                     VYFGEAIRILDWDMEAAAREFCRAVALDPNSTPSNYFSAAFWAARGEREEALMYLRRT
                     ARIDPASLWVNNSACELYRYFGLYDEAIAAGERALQLDPAFVYGEPLLAALYREMGRF
                     DEAIALYEKAQNLSGRPAFGLAITYARMDRHDDALRALEAATANRRYTPGDAIAHVRV
                     ALGEHDEAIRELQRACDERSSSLHFVGIAPEFVPLRTHERFVSILQKIGLQPEKVFAA
                     TAV"
BASE COUNT          617 a          964 c          990 g          710 t
ORIGIN      
        1 ttgccgaggc gtttgtgtag ttattccact gcgccgtagc ggcgctggcg acgcccgtat
       61 tcgtccacgg ccgcgaaaag atcttttttc gtgaagtcgg gccaaagccg cggcgtgaca
      121 taaatttcgg tgtaggaaag ctgccagagc aagaaattcg ataaacgcat ttcgccggag
      181 gtgcggatga gcaggtcggg gtccggatag taacgggtgt aaagatgctt actgaacatg
      241 tcgacatcga tcatcgccct gtcgatgtgg ccgagctcga tcgcgcggag caggctttta
      301 attccgtcaa tgatctcgag ccggccgccg taactcagcg ccaggattaa tgtcagccca
      361 ttgttcacag cagtttgctc gatcgtcttg tgcagttgtt cctggcaact ctggggcaga
      421 tcggttagcc ggccgatggc ctggagtcgc acgtttttct ccaacaactc gggcgtcttc
      481 tctttgagga atttctcaag cagccgcatc aatgccagta cctcaccctt gggccgctgc
      541 cagttctcag cggaaaaggc atacagggtg aggtattcca ctttcagttc ggcgcagcct
      601 tcgacacatt cgcgcgccgc ttgcgcgcct tgctcgtgac ctttgatccg cggcagtccg
      661 cgcgctcgcg cccagcggcc gttaccgtcc ataataatcg cgatgtgtcg cggaaccgct
      721 ttggtgctcg cggccatcgc tacagttgga ttgtctgccg gcgtccagga ccaacgctca
      781 cgatcgagag acgcgcgccc gtgagctcgg aaatgcggcg gacgtattct ttcgccgcgc
      841 gcggaagctg cgaaaacttg cgcgcagcgt gcgtggattg tttccagccg cgcatctgct
      901 cgtaaatcgg ctcgcaattg tcgagctggg aagcgtccga aggcggcaca tccagccgct
      961 tttcattcag ccgataaccg acgcagacgc ggattggatc gacagcgtcc agtccgtcca
     1021 aattcgtgat cgccagctcg tcgattccgt tgatcatgcg cgcatagcgc gtcgcgaccg
     1081 cgtcaaacca accgcagcgt cgcgcccgtc cggtggtcgc gccgaactcg cgcccgagct
     1141 tgtgaagcat ttcggcaaat tgtcgatctt cggtcggcaa tgggccttcg ccgacgcgtg
     1201 ttgtgtaggc tttcatcacg cccaggacgc ggtccatgcg atgcggtggc acgcctgtgc
     1261 cggtgcaggc gccgccagcc gtggtattcg acgatgtgac atacggataa gtgccatggt
     1321 caatgtcgag aaacgtcccc tgcgcgcctt caaagagaat ttctttgttt ccttcgagcg
     1381 cgcgatgcag atagacgacg gtgttcgcga tgaacgggcg cagcttttcc gcggctgtga
     1441 ggtaactccg gttgacgtcg cggaaactga tcgagtgcgc gcccagcgcc tggaggatgc
     1501 tgttgttctc aacaattttc gcctgcagct ttttcgagaa cagaattggc tgcatcagat
     1561 cggagatgcg cagaccggtg cgtgcggctt tgtcgccata ggctgggccg atgccgcgct
     1621 tggtcgtgcc gagtttagcg cggcctttgc ggagttcccg ctgctcatcg agcagtcggt
     1681 ggtatggcag aaccagatgc gcgcaatcgc tgatgagcag gttatgatcg atcttgatgc
     1741 cgcgcttgcg caaaccctcc atttccgcga cgagcgcgat gggatcgatc acgacgccgt
     1801 tcccgatgac gcagactttg ccgcgccgca ggatgcccga cggaataaga tgcaggacgt
     1861 acttaatgcc gcgatgaatg acggtgtgac cggcgttgtt gccgccctga ctgcgcacga
     1921 cgatgtcggc cttagccgtg agcacgtcga tgatcttccc cttcccttca tcgccccatt
     1981 gcgcgccgat taagatcgtg ttcgccatct gccgaatcgc tgggcagaaa aattgccccg
     2041 atgattctcc gagcgtcgcc gagccttgca aattaaaaca ttgccatcga gatcaacgag
     2101 cgagtagcct gtcgagatgc gcacaattct gctcataatt ctcgtgcttc tactgatcgg
     2161 ggcattgccg acctggccgt acagcaccgg ctggggctat tatcccagtg gtgggctcgg
     2221 tttgatcttg ttgatcgtgc tgatcctcgc tctggccggc cggctctaga ccgcagttgc
     2281 cgcgaagacc ttttccggtt ggagcccgat cttctgcaag atcgagacga aacgttcgtg
     2341 cgtgcgcaga ggcacgaact ctggggcgat cccgacgaaa tgcagcgagg aggagcgctc
     2401 gtcgcacgcg cgttggagct ctcgaatcgc ttcgtcatgt tctccgaggg cgacgcgcac
     2461 gtgtgcaatt gcgtcgccgg gcgtgtagcg ccggttggcg gtcgcagctt cgagcgctcg
     2521 gagcgcgtca tcgtgccggt ccatgcgggc ataggtgatg gcgagaccaa aggcgggtct
     2581 gccgctgaga ttttgcgcct tttcgtagag cgcgatcgct tcgtcgaatc gtcccatctc
     2641 ccgataaagc gccgccagca acggttcgcc atagacaaat gccgggtcga gctgcaaagc
     2701 gcgttcgccc gctgcgatcg cttcatcata caggccgaag taacggtaga gctcgcaggc
     2761 agaattgttc acccagagcg aggccggatc gatcctggcg gtgcgccgaa gatacatcag
     2821 cgcttcttcg cgttcgccgc gggcagccca gaaggccgcc gaaaaataat tcgaaggcgt
     2881 tgagttcgga tcaagggcca cggcacggca aaactcgcgc gcggctgctt ccatgtccca
     2941 atccaggatg cgtatcgctt cgccgaaata cacatgcgcc tcggcatcgt cattgtcgat
     3001 cttgagcgcg ttggtcgccg catcacgcac tttcggatac gcctcgcgcg gcggcacata
     3061 agcgtccgcc agccataacc acgctttcgc gatccccgtc caggcgcgac cgaattgcgg
     3121 atcgagctca agcgcgcgct cgaagtattg caggcttgtc cgcagcgctt cctcggtggt
     3181 tttgtcagaa taaaagagac cgtgcagata tagatcgtag gactggctgc tgtggacgag
     3241 ccgcgacatc ggcggcgcgg agacgtcgag tttcaatttc a
//
LOCUS       DASTDG010000004         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_971931, whole genome shotgun sequence.
ACCESSION   DASTDG010000004 DASTDG010000000
VERSION     DASTDG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971931"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFO30_00090"
     CDS             complement(<1..229)
                     /locus_tag="VFO30_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9417714.1"
                     /translation="MADESVSLVNVQIDGAWRQFPKGTRVIEACEQAGSYVPRYCYHK
                     KLSSPGNCRMCLIEMGMPRMGPDRKPELGPDG"
     gene            complement(257..685)
                     /locus_tag="VFO30_00095"
     CDS             complement(257..685)
                     /locus_tag="VFO30_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9417715.1"
                     /translation="MSQESRNAGTQLRDSETTQAIIAAAIAVHCELGPGFLESIYEQA
                     LAVEFALRGIAFVRQKPIPLFYRDHQIGEHRLDFVVEAKIVVELKAVEALENVHFAIV
                     RSYLKATGLTDGLLLNFSTMPLTVKRVRRERTFEAPDLQK"
     gene            complement(689..2794)
                     /gene="nuoF"
                     /locus_tag="VFO30_00100"
     CDS             complement(689..2794)
                     /gene="nuoF"
                     /locus_tag="VFO30_00100"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9417716.1"
                     /translation="MDSTSTRVPAELEQRMDEAIGPFPAEHKRSAALPLLHLWQEHFG
                     FISDEAVEWIAAKLGLQPINILELVTFYPMLRQTSAGKTHIRVCRTLSCAMAGSYQLM
                     DNLCAATGIQRKRDSKLDCLKQSSLTNGAERELDGLKPSSLTSGVAYGVASASDNDEI
                     HNPISVSPDGNYSIEFVECLASCGTAPVCMVNEELHENVDAFSAASLIQNRESRIANR
                     PEPHPLEKRLVFKNIGRPDWKIDIDTYIYDGGYEQLRKAVTMSRADIVNEVKTSGLRG
                     RGGAGFPCGVKWSFIKPDEKKPVYLICNADESEPGTFKDRYIIHEDPHQLLEGTLISA
                     YAVGAKTVYIYIRGEFAEGAKILARAIEEARDRNFLGKNILGSGFDCEIYIYRGAGAY
                     ICGEETGLIESLEGKRAYPRIKPPYFPAVLGLYMCPTIVNNVETLCHVKHIIAMGGAA
                     YTRLGRPNNTGTRIVCVSGDVQRPGYFEIEVGKVTMGQLIYDMAGGLRPGRTLKAVIP
                     GGSSAKVLRADERFKIKDRQLDGSTVEREMTIDEIPMDFDSLAAAGSMAGSGGVIVLD
                     DSCDMVWALNNINKFYAHESCGQCTPCREGALWMQKITDRMLAGGGVTEDPQTLKKIG
                     DNIAGRTICAFGEACAWPTQSFVEKFPEEFAMRAQKPAPPPLPPEYTPEELIEEEKIP
                     TTPLAHDPGWEKAGAAGTI"
     gene            complement(2806..3093)
                     /gene="bamE"
                     /locus_tag="VFO30_00105"
     CDS             complement(2806..3093)
                     /gene="bamE"
                     /locus_tag="VFO30_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HET9417717.1"
                     /translation="MSILQTLSRCMLVLMLMFVLGSCTGPRITKTNVDQVTEGMSKKQ
                     VESILGPPTDIDNKDFVIMKKTTYVYRQGKDTVTVVFKDDKVTEKQSTLSD"
     gene            complement(3096..>3758)
                     /locus_tag="VFO30_00110"
     CDS             complement(3096..>3758)
                     /locus_tag="VFO30_00110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9417718.1"
                     /translation="LRDFLDHFGPALDEMDKLLTRNRIFVDRTKEIGVIPRERAIAYA
                     LSGPNLRGSGVEHDLRRKHPYLDYEQYDFEVVIGSAGDCYDRYLVRIEEMRQSVRILR
                     QVADKIPGGPINVADWKNMLPPKAHVMTKMEELIHHFIIVTEGLDAPPGEIYFGAENP
                     KGELGFYINSKGGGVPHRLKIRAPSFVNLSILPELLPGCMVSDVPAVLGSLDFVMGEC
                     DR"
BASE COUNT          752 a         1095 c         1026 g          885 t
ORIGIN      
        1 gaccatcggg cccgagctcc ggtttccggt cgggacccat tctgggcata cccatttcga
       61 tcaaacacat ccggcaatta ccgggcgagc tgagcttttt gtgataacag taccgcggca
      121 catagctgcc ggcctgctcg cacgcctcaa tcacgcgcgt gcccttcggg aactgccgcc
      181 acgcgccatc aatctggacg ttgacgagcg aaactgattc gtcagccata tctcaggaaa
      241 gcaggaacgc aggaaatcat ttctgtaggt cgggagcttc gaacgttcgc tcacggcgaa
      301 cacgtttcac ggtcaacggc atggtcgaaa agttcagcag cagtccatcg gttaatccag
      361 tcgctttcaa atagctgcga acgatcgcga aatggacgtt ctcgagcgcc tcaactgctt
      421 tcaattcgac gacgattttg gcttcgacaa cgaagtcgag tcgatgctcg ccaatttgat
      481 gatctcggta gaaaagtgga ataggcttct gcctaacgaa cgctatgcca cgcaacgcga
      541 actcgacggc taacgcttgt tcgtagatcg attccagaaa acccggcccg agttcgcagt
      601 gcactgcaat cgcggcggca atgatcgcct gtgtcgtttc tgaatcgcgt aattgtgttc
      661 ctgcgttcct gctttcctga gacatagatc agattgttcc ggccgcgccg gctttttccc
      721 agccaggatc gtgggcgagg ggagttgtcg ggatcttctc ttcctcgatc agttcttcgg
      781 gcgtgtattc gggtggcagc ggcggtggcg cgggtttttg cgcgcgcatg gcgaattctt
      841 ccgggaactt ttcgacaaag ctttgcgttg gccatgcgca cgcttcgccg aaggcgcaga
      901 tcgtccggcc cgcgatgttg tccccgattt tctttagcgt ctgcggatct tcggtcacgc
      961 cgccgccggc cagcatgcgg tcggtgattt tctgcatcca aagcgcacct tcacggcatg
     1021 gtgtgcactg gccgcacgac tcgtgcgcat aaaatttgtt gatgttgttc aacgcccaaa
     1081 ccatgtcgca cgagtcatcg agcacgatga caccgcccga gccggccatt gacccagcag
     1141 cggcaagcga atcgaaatcc atcggaattt cgtcgatcgt catttcgcgt tcgactgtcg
     1201 atccatcgag ttgtcgatct ttgattttga atcgctcgtc cgcgcgcaac actttcgcag
     1261 agctgccgcc agggatcacc gctttcaatg tgcggccggg ccgcaaaccg cccgccatat
     1321 catagatcag ctggcccatc gtcactttgc caacctcaat ctcgaaataa cccggccgct
     1381 gcacatcacc gctgacgcaa acaatgcgcg tgccagtgtt gttaggtcga ccgagtcgcg
     1441 tgtaggcggc gccgcccatc gcgatgatgt gcttcacgtg acaaagcgtc tccacgttgt
     1501 ttacgattgt cggacacatg tagagaccga ggaccgctgg aaagtacggc ggcttaattc
     1561 gcggataggc gcgcttacct tcgagcgact cgattaaccc ggtctcttcg ccgcagatgt
     1621 aagcgcccgc gccgcgatag atataaatct cacagtcgaa tccgctgccg aggatgtttt
     1681 tcccgaggaa atttcggtcg cgcgcttcct cgatcgcgcg ggcgaggatc ttggcgcctt
     1741 cggcgaactc gcctctgatg taaatgtaaa ctgtctttgc gccgacggcg taagccgaga
     1801 tcaacgtgcc ttccaacaat tgatgcgggt cctcgtgaat gatgtagcga tccttaaaag
     1861 tccccggctc ggattcgtcg gcgttgcaga tcaagtagac cggcttcttc tcatcgggct
     1921 tgatgaaact ccatttcacg ccgcacggaa aaccggcgcc gccgcggccg cgtaagcccg
     1981 acgttttcac ttcattgacg atgtcagcgc gcgacatcgt cacggctttc ctcaactgct
     2041 cgtaaccccc gtcgtagatg taagtgtcga tgtcgatctt ccaatcgggg cggccgatgt
     2101 ttttgaatac cagtctcttt tccagcggat gcggctccgg tcgattcgcg attctcgatt
     2161 cacggttctg aataagactg gcagccgaga acgcatcgac attttcatgg agctcttcgt
     2221 tgaccatgca aacgggcgcg gtgccgcagc tggcgagaca ttcgacgaac tcaatcgagt
     2281 aatttccgtc cgggctgacg gagatcggat tgtgtatttc gtcgttgtcg ctcgcgctgg
     2341 ccacgccgta cgcaacgccg ctcgttagac tcgacggctt taagccgtcg agttcacgct
     2401 ccgcgccatt cgttagactt gactgcttta agcagtcaag tttgctgtcc cgcttgcgct
     2461 ggatgccggt tgcggcgcag agattgtcca tcagctgata actgccagcc attgcgcagc
     2521 tcagtgtccg gcacacgcgg atgtgcgttt tgcctgcgga tgtttgccgt agcatcgggt
     2581 aaaacgtgac tagctcgaga atgttaatcg gctggagccc gagcttggcc gcgatccact
     2641 ctacggcttc atcgctgatg aatccgaaat gttcctgcca cagatgcagc aacgggagcg
     2701 cggcgctgcg tttgtgctcg gccggaaaag ggccgatcgc ctcgtccatt ctttgctcca
     2761 actcggcggg aacgcgtgtg ctcgtcgagt ccacggaaaa cgagattaat cggagagcgt
     2821 gctttgtttc tcggtgacct tgtcgtcctt gaaaacgacc gtcacagtat ctttgccctg
     2881 tcgatagacg taagtcgttt tcttcatgat gacgaaatcc ttattgtcga tatcggtggg
     2941 cgggccgagg atcgattcca cctgtttctt cgacatgccc tcggtgactt gatcaacgtt
     3001 ggttttggtg atgcgagggc ccgtgcaact gccgagcacg aacattagca tgagcacgag
     3061 catgcatcgc gaaagtgttt gcaagatcga catgttcatc tgtcgcattc gcccatgaca
     3121 aagtccagac taccaagtac cgccggcacg tcgctcacca tgcatcccgg taacaactcg
     3181 ggcaagatgc tcaggttcac gaatgacggc gcgcgaatct tcagccgatg cggcacgccg
     3241 ccacccttgc tgttgatgta aaaaccgagc tcgcccttgg ggttttccgc cccgaagtaa
     3301 atctcacccg gcggcgcgtc gagtccttcc gtcacgatga tgaaatggtg aatcagctcc
     3361 tccatcttcg tcatcacgtg tgccttcggc ggcagcatgt ttttccaatc cgcgacattg
     3421 attgggccgc cgggtatctt gtcggcgacc tgacgcagaa ttctgacgct ttgccgcatc
     3481 tcttcgatcc ggacgaggta tcgatcgtaa caatcgcccg cgctcccgat cacgacttcg
     3541 aagtcatact gctcgtaatc gagatacgga tgtttgcgcc gcaaatcgtg ttcgacgccg
     3601 ctgccgcgca ggtttgggcc ggacaatgcg taggcgatcg cgcgttcccg cggaatcacc
     3661 ccgatctctt ttgtgcgatc gacaaagatg cgattgcgcg tgagcaattt gtccatctca
     3721 tccagcgccg ggccgaagtg atcgagaaaa tcacgcaa
//
LOCUS       DASTDG010000005         8577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1642036, whole genome shotgun sequence.
ACCESSION   DASTDG010000005 DASTDG010000000
VERSION     DASTDG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642036"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..128)
                     /locus_tag="VFO30_00115"
     tRNA            complement(57..128)
                     /locus_tag="VFO30_00115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Val,seq:gac)
     gene            complement(267..818)
                     /locus_tag="VFO30_00120"
     CDS             complement(267..818)
                     /locus_tag="VFO30_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9417719.1"
                     /translation="MHNLLTVKETAKYLRIPLPTVYYLVQRGQLPAIQIGGRWRIKKS
                     SLDKDILKEEKSGQPTVLVVDDDESLQNLFKVFLKKIGFSRIVVGTVKEALAALEKQK
                     FDLIFLDLKLPDGPADDVYDTARAEQPNCPIVIITGYPDSEMLDRILAKGPITVLKKP
                     LKAEQLQQTVRILGHKEAVKLAA"
     gene            1015..1650
                     /locus_tag="VFO30_00125"
     CDS             1015..1650
                     /locus_tag="VFO30_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HET9417720.1"
                     /translation="MTIRTRMSVLFFKRFLKRPLQIASIVPSSHALVQRVASKMDFSQ
                     PRVVAEYGPGDGVHSREIARRITADSHLLLFELDRAFARELQRQFAPDPRVHVIHGDA
                     VSLPEELERRGFDKCDYILSGIPFSILKIDKKRALLQKTYDALAPGGRFIIYQVTNEL
                     RQHATLFDRAQSEYFLQNIPPMFITVFQKAIRDGAHPADAELSRIAVNRQA"
     gene            1647..3062
                     /locus_tag="VFO30_00130"
     CDS             1647..3062
                     /locus_tag="VFO30_00130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9417721.1"
                     /translation="MTEHGTRVEKDSMGEMSVPAKALYGASTQRAVLNFPISGYRFSR
                     PFIRALGLIKWAAAQANHDLGLLDAERSALIVQAAEEVIEGKLDEHFPLDIFQTGSGT
                     STNTNANEVIANRCAQLAGKAIGSRELVHPNDHVNMGQSSNDVIPSAIHISAAEQLKH
                     CLIPALGKLHSALERKTQEFWDVIKIGRTHLMDATPVRLGQEFSGYAQQIRYGKARAE
                     KALEVLRELALGGTAVGTGLNRHVDLTGKMLRHLEQRTGIAFYEAGNHFEAMGGKDAV
                     VEVSGELKTIATSLFKIANDIRLLGSGPRCGIGEIQLPPTQPGSSIMPGKVNPVLCES
                     LMMVCAEVFGHDATVTWSGANGNFELNVMMPVMAHDLLESIRLLANAVDAFSEKCVTG
                     MTANEKRCAELVELSMAMVTSLAPKIGYDRAAEIAKESARTGRTVREICRERKILPEA
                     ELDRVLDPLSMTEPVGKTQDR"
     gene            3062..3565
                     /locus_tag="VFO30_00135"
     CDS             3062..3565
                     /locus_tag="VFO30_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417722.1"
                     /translation="MNLRILFVIALLAVGAQHDAAIAADSHIDVSHIVDQTTAESILG
                     EPVKSATPRNVDGKDGYYSKCNYYTSKPGKALVVRVYQAAPGTDPQTALEAVTESTGA
                     MTTVPGLGDRARLSSGAEGGLPLHVVMLYVVKGNALVTVGLSGLDDDVLAAEKTKTVA
                     QKVLASL"
     gene            3586..3963
                     /locus_tag="VFO30_00140"
     CDS             3586..3963
                     /locus_tag="VFO30_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417723.1"
                     /translation="MVAKLEGPQFSGGANIAIKCPSHTYDETVAFYRDTLALPLIEEE
                     AEGCIFQFGPNRLWIDKVPNLSHPDIWLELETNDTEAAAAFLKVHGVTRRDEVELLRE
                     DLNGFFIAAPNGVIHLVIAQDEA"
     gene            complement(4012..5232)
                     /locus_tag="VFO30_00145"
     CDS             complement(4012..5232)
                     /locus_tag="VFO30_00145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9417724.1"
                     /translation="MSVASSDHRRSETGATDIRVRDARNVDLAAITDIYNAAIATRSA
                     TAQLEPVTIDECRGWVSGGYPLWVAEIDGCVAGWLSCKPFLPRCAYRGTVELSVYVDR
                     SFRRRGVARRLLQHAIARGPALGISAMVGLIFEDNAPSLRLFQRLGFARWGVLPRVAR
                     VEAAERDLVIVGHHCDARHIDAPVNDAEAQLHEAFELMHSLMQYRQQPIHGELDNVRK
                     KYSMLHLDVLLLIYHFAKIGSGQILEIGAYLGGATIAAARGVRDSGKQKTVIAVEQGG
                     SLKHERLGTRNILRDLERNLAKARVADLVRLIKGRSCDTATVAAVEQALGPDKICLLI
                     LDADAAKQRDIDCYRAKLATGCWMVIDDYYGPVEKIAAARADVDSLTAAGWLEPLGFY
                     GWSTWVGRWRGTES"
     gene            complement(5229..6092)
                     /locus_tag="VFO30_00150"
     CDS             complement(5229..6092)
                     /locus_tag="VFO30_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9417725.1"
                     /translation="MFSLTMLGSGSGGNSALVATDHCRVLVDGGLSARQIVFRLEQCG
                     VTPEQLDGVLLSHEHGDHVCGLGVLCRKFDIPIYCNALTAEAIRCDCSLDRHRNWRIF
                     RTGAEFSICDITIQIFPVPHDAVDPVGFAFHAGSGSLGFITDLGYAPRVIIERLRDVR
                     TIVIETNHDEKLLQNDSHRPWPVKQRIQSRHGHLSNTAAASVIEQLLPGKIERVVLGH
                     LSRDCNTPALAFAAVRESIMRAGKIDIELHCATQASVSARFPLGETPRGAFQPTFETT
                     FFQTANHSGAV"
     gene            complement(6152..6880)
                     /locus_tag="VFO30_00155"
     CDS             complement(6152..6880)
                     /locus_tag="VFO30_00155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9417726.1"
                     /translation="MGRTRLLPRVWRHRRKRASQLEPWPIPLYGIWDEIAGFAAEIRA
                     SDFMAEMMRRRAAHGSAGLMGALDCATLYALTRWRRPRGVVESGGFVGLAASFILKAL
                     ADAGVMDAKLYSIESDENCAHGELIPDPLRRQFVPLRAKVQDVIRADTLPPKIDMFLH
                     DSSHRYRHMLWEFREFWPRLRDGGLLVSHDVHMNAAFASFVVETYAHDKKTGVLDRSR
                     TAHEEWGRWGYIGFMIKKADRANV"
     gene            complement(6933..7628)
                     /locus_tag="VFO30_00160"
     CDS             complement(6933..7628)
                     /locus_tag="VFO30_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417727.1"
                     /translation="MNETLFSQIQKLLERTYAQVGINLEECLIDRERCKQLSILAGTS
                     ARELSELARTFLRHTDDRLYVGIYYSRWLIEELERHDPRAGLGDHNIRSLIVFVEEIN
                     HALHAALQFRNGAREIASEDFARNLELQAQVDTYLVLLLFIAFFRKTQRVSRSDRRWL
                     RFHLFARQCPDAFRDENLRGRYLETTELAASYTQFLDSLNGVRRLEEIRAFRSLDYAD
                     KKRRILALADRAL"
     gene            complement(7625..8314)
                     /locus_tag="VFO30_00165"
     CDS             complement(7625..8314)
                     /locus_tag="VFO30_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417728.1"
                     /translation="MIRANCRERFTAADFDFIVRTLARSQGNHVSLVDLLSDGEARDS
                     VLDNPRLIDAILSHPMQLRISPQFYFYVLARHVLRQAAINDRKLCDYVASLLETFSRA
                     SQLERPADIGNHAHEYICDMLIALQRVTPEQAFLLRAHIGNYSLFISGIFHESTQRRH
                     ERGGPDLKFYEQIGRTNYQLVASHETARRCELDDVFERLAERFRDVRLALNELSDRLL
                     NLDDVRPSLAL"
     gene            8482..8571
                     /locus_tag="VFO30_00170"
     tRNA            8482..8571
                     /locus_tag="VFO30_00170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8518..8520,aa:Ser,seq:cga)
BASE COUNT         1840 a         2466 c         2539 g         1732 t
ORIGIN      
        1 taaccgagcc gcgaaatcgg ttggagaccg tccagcggta atcaacggtg aggaaatgcg
       61 cgatactggg ttcgaaccag tgacccctac cgtgtcaagg tagtgctcta ccactgagct
      121 aaccgcgcgt tttcgaccga agtgataagt gatcggtgat cgctgatccg tcaagatcga
      181 gcagtgatcg tcgatcgagc gggtgaacta ctgaactacc gattactatt caccgatcgc
      241 ggatcacaaa actcgcgcag cgagtttcag gcggcgagct tgaccgcttc cttgtggccg
      301 aggatgcgga cggtctgttg gagttgctcg gccttgagcg gtttcttcag aacggtgatc
      361 gggcctttcg ccaggatgcg gtcgagcatt tcgctgtccg gatacccagt gatgatgacg
      421 attgggcagt tcggctgttc cgcccgggcg gtgtcgtaca cgtcgtcagc cggcccatcc
      481 ggcagtttca gatcgagaaa gatgaggtcg aacttctgtt tctcgagcgc ggccagcgct
      541 tccttcactg tcccgacgac gatgcggctg aagccgattt tcttgagaaa aactttaaac
      601 aaattttgca aactttcgtc atcatcgacg acgagcacgg tgggctggcc ggacttctct
      661 tctttcagaa tgtccttatc gagcgacgat tttttaatcc gccaacgccc gccgatttgg
      721 atagcgggaa gctgcccgcg ctgaacgagg taataaacgg ttggcagcgg gatgcggaga
      781 tacttggctg tttccttgac ggtaagcagg ttgtgcatgg gctcgtcaat tagtcagcaa
      841 tttggccggg tgggggcaag ctaattcgca accgccaagc attataaatt ttagaatcgg
      901 tgtcaatcct ttgttaggcg ctgccgctgg cacggatcgc caagcaccgg cgcgataaca
      961 cgcttttgcc gcgagaagta gccacttcgc ggctggacaa tgccggagcg gcgagtgact
     1021 attcgcactc gcatgagcgt gctctttttc aaacgtttcc tgaagagacc gctacagatc
     1081 gcctcaatcg tgccgagctc gcatgctttg gttcaacggg tcgcgagcaa gatggacttt
     1141 agccaaccgc gtgtggtcgc cgagtacggt ccgggcgacg gcgtgcactc gagggagatc
     1201 gctcggcgga tcacggccga ctcgcatctt ctgctgttcg agctcgaccg ggcgttcgcg
     1261 cgcgaactcc agcgccagtt cgcacccgat ccacgcgtgc atgtcattca cggcgacgcc
     1321 gtctcattgc cggaagaact cgagcgccgt ggtttcgaca aatgcgatta cattctctcg
     1381 ggcatccctt tcagcattct caagatcgac aaaaaacgcg cgctgttgca gaaaacctac
     1441 gacgcgctcg cgcccggcgg caggttcatt atttaccagg tcaccaacga attgagacag
     1501 cacgcgacct tgttcgaccg cgcccagtcg gagtatttcc tgcagaacat cccgccgatg
     1561 ttcatcaccg tttttcaaaa ggcgatacgc gacggcgctc acccggcaga tgcagagctt
     1621 agccgtatcg cggtaaaccg gcaggcatga cggagcacgg gacgcgagtg gaaaaagact
     1681 cgatgggcga aatgtctgtc ccggcgaagg ctctttacgg tgcttccacg caacgcgccg
     1741 tgctgaattt cccgatcagc gggtatcggt tttcgcggcc gttcatccgc gcgctgggtt
     1801 tgatcaagtg ggccgccgcg caagcaaatc acgatctcgg gttgctcgac gcggaacgca
     1861 gcgcgctgat cgtccaggcg gcggaggaag tgatcgaggg aaaactggac gagcatttcc
     1921 cgctcgacat ttttcagacc ggctccggca cgagcacgaa cacgaacgcc aacgaagtga
     1981 ttgcgaaccg gtgcgcgcag ttggccggga aagcgattgg ttcgcgtgag cttgtgcatc
     2041 cgaacgatca tgtcaacatg ggccaatcaa gcaacgacgt gatcccgtcg gccattcaca
     2101 tcagcgcggc cgagcaactc aagcactgtc tcatccccgc gctcggcaag ttgcacagcg
     2161 cgctagagag aaagacacag gaattttggg acgtgatcaa gatcggccgc acccacttaa
     2221 tggacgcgac gccggtgcgg ctcggtcagg aattttccgg ttacgcgcag cagattcggt
     2281 acggcaaagc gcgggcggaa aaagcgctgg aagttttacg tgagctggcg ctcggtggca
     2341 cggccgtcgg caccggcctc aatcgacatg tcgatcttac cggcaagatg ctgcgccatt
     2401 tggaacagcg gaccgggatt gcattttacg aagcgggcaa ccatttcgaa gcgatgggcg
     2461 gtaaagacgc ggtggtcgaa gtgagtggcg aactcaagac aatcgccacc agtctcttta
     2521 agatcgcaaa cgacattcgc ttgctgggca gcggtccgcg ctgcggcatc ggcgaaattc
     2581 agctgccgcc gacgcagccg gggagctcga tcatgcctgg gaaagtgaac ccggtgctgt
     2641 gcgaatcgct catgatggtg tgcgccgagg tgtttggcca cgatgcgacg gtcacgtggt
     2701 cgggtgcgaa cggcaacttc gagctcaatg tcatgatgcc agtgatggcg cacgatcttc
     2761 tcgaatcgat tcgactcctc gccaatgcag tcgacgcatt ctctgagaaa tgcgtgacgg
     2821 gcatgaccgc gaacgagaaa cgttgcgccg aactagtcga gctgtcgatg gcgatggtca
     2881 ccagcctcgc tccgaaaatc ggttatgacc gcgccgcgga aatcgcgaag gaaagcgcca
     2941 ggaccggccg cacggtgcgc gaaatctgcc gggaacgaaa aatcttgccg gaagcggagc
     3001 tcgatcgtgt gctcgacccg ctcagcatga ccgagccggt gggaaaaacg caggatcgtt
     3061 aatgaacctg cgtattctgt ttgttatcgc tctacttgcc gtcggcgctc aacacgacgc
     3121 agcaatcgcc gccgattcgc acatcgacgt ttcgcacatt gtcgatcaaa caacggcgga
     3181 atccattctt ggcgaaccgg tgaaatcggc cactccgcga aatgtcgatg gcaaggatgg
     3241 ctattactcg aagtgcaatt actacacgtc gaaacctggt aaagcgcttg tcgttcgcgt
     3301 gtatcaagcg gcacccggaa ccgatccgca aacggcgctc gaagccgtga ccgagagcac
     3361 cggcgcgatg acgacggtgc ctggcttggg cgacagagca cggctctcga gtggagcgga
     3421 gggcgggctg ccgctgcacg tcgtcatgct ttacgttgtc aaaggcaacg cgttggtcac
     3481 ggttggcctc tccggcctcg atgacgacgt gctagctgcc gaaaaaacga aaactgtcgc
     3541 gcagaaagtc ctcgccagtc tgtaatcgtc tcgcctcttt cgcacatggt tgccaaactc
     3601 gaagggccgc agttttccgg gggcgcgaat attgcgatca aatgcccgag ccacacctat
     3661 gacgagacgg tggcgtttta tcgcgatacg cttgctttgc ctctgatcga ggaagaagcc
     3721 gaaggctgta ttttccagtt tggcccgaat cggctttgga tcgacaaagt gccaaacctc
     3781 agtcatcccg acatctggct tgagctggag acaaacgaca ccgaagcggc cgcggcgttc
     3841 ctcaaagttc acggcgtgac acgccgcgat gaagtcgaac tattgcgcga ggatttgaat
     3901 gggttcttca tcgcggcgcc gaacggggtg attcatctcg tcattgctca ggacgaggcg
     3961 taacgcgagc gcgtgattgt cgatcttagc gggcgcgttc cccgcgcaga tttatgattc
     4021 agttccgcgc cagcggccca cccacgtgct ccagccgtag aacccgagcg gctccagcca
     4081 gccagctgcc gttaacgagt cgacatcggc tcgcgcggcg gcgattttct caaccgggcc
     4141 gtagtagtca tcgatcacca tccaacagcc ggtggcgagt ttagcgcgat agcagtcgat
     4201 gtcgcgctgc ttggccgcgt cggcatcgag gatgagcaaa cagattttat cgggaccgag
     4261 cgcttgctcc acggccgcga ctgtcgccgt gtcgcacgag cgtcctttaa tcagcctcac
     4321 cagatcggcg acgcgcgctt tcgcgaggtt tcgctccaga tcgcggagaa tgttgcgcgt
     4381 gccgaggcgc tcatgtttca ggctgcctcc ttgttcgacg gcaattactg ttttctgttt
     4441 gccggaatcg cgaacaccgc gcgcggcggc aatggtcgct ccaccgaggt aggcaccgat
     4501 ctccagaatc tgtcccgaac cgatcttggc aaaatggtag attagaagca ggacgtcgag
     4561 atggagcatc gaatattttt tccgaacgtt gtcgagttcg ccgtggatcg gttgctgccg
     4621 gtactgcatc agcgagtgca tcaactcgaa tgcttcatgt agctgcgctt ccgcgtcgtt
     4681 gactggggcg tcgatgtgtc gcgcatcaca gtgatgtccg acgatgacaa ggtcacgctc
     4741 ggccgcttcg acccgcgcga cccgcgggag tacaccccag cgggcgaacc ccagccgctg
     4801 aaataagcgc aggctcggtg cattgtcttc gaagatcagt ccaaccattg cgctgatgcc
     4861 gagcgccggg ccgcgcgcga tcgcgtgttg aagtaatcgt ctcgccacgc cgcgccgacg
     4921 gaagcttcgg tcgacgtaca cgctcaattc gactgttccg cggtacgcgc agcgcggcag
     4981 aaacggcttg caactcagcc aaccagcaac acacccatca atctcggcga cccacagcgg
     5041 ataaccgccg ctcacccacc cgcggcactc gtcgattgtg accggctcta attgtgcggt
     5101 ggcgctcctt gtcgcaatcg ccgcattgta aatgtcggtg atcgccgcaa gatcgacgtt
     5161 tcttgcatcg cgcacccgaa tgtctgtcgc gccggtctcg gatcgccgat gatccgacga
     5221 agcgacgctc atacagcgcc gctatggttc gctgtctgga aaaatgtggt ttcaaatgtc
     5281 ggctgaaacg cgccgcgtgg ggtttcgccg agtggaaagc gtgcactgac gctcgcctgt
     5341 gtcgcgcaat ggagctcgat gtcgatcttg cccgcgcgca tgatcgattc ccgcaccgcc
     5401 gcaaaggcca gcgcgggtgt gttgcaatca cggctgagat gaccgagcac gacacgttcg
     5461 attttcccag ggagcaattg ttcgatcaca ctcgctgctg ccgtgttcga cagatgcccg
     5521 tgccgcgatt ggattcgctg tttcaccggc cagggccgat gcgaatcgtt ctgcaaaagt
     5581 ttttcgtcat gatttgtctc aatcacgatc gtacgcacgt cgcgcagccg ctcgatgatg
     5641 acgcgcggcg cgtagccgag atcggtgata aagccgagac tgccggagcc agcgtgaaac
     5701 gcgaacccga caggatcgac cgcatcgtgg ggcaccggaa aaatttggat ggtaatgtcg
     5761 cagatcgaaa attcggcgcc ggtgcggaaa atccgccagt tccgatgtcg atctaatgaa
     5821 cagtcgcagc ggatcgcctc ggcggtgagg gcattgcaat agatcgggat gtcgaactta
     5881 cgacagagaa ctcccaggcc gcagacatga tcgccgtgct cgtgactgag caacacccca
     5941 tcgagttgtt ccggtgtgac gccgcattgc tcaagccgga aaacaatctg gcgcgcgctc
     6001 aagcccccat cgaccaacac tcggcaatgg tcggtcgcga caagcgcgct gttaccgccg
     6061 ctgccgctgc cgagcatcgt taagctgaac acgacgggta tttagcgacc ggcgtgcaat
     6121 tttcgcaaac gaaattccgg cgcgccgctg ctcacacatt cgcgcgatca gcttttttaa
     6181 tcatgaaccc gatgtaaccc cagcggcccc attcttcatg cgcagtccgc gaccgatcga
     6241 ggacaccggt ttttttgtcg tgcgcgtatg tctcgaccac gaagctcgca aacgcggcgt
     6301 tcatatgcac gtcgtgcgag acaagcagcc cgccatcgcg gagccggggc cagaattcgc
     6361 gaaattccca aagcatgtgt cgataccggt gcgagctatc gtgcaggaac atgtcgatct
     6421 tcggcggcaa tgtgtcggcg cgaatgacgt cttggacttt tgcacgcagc ggcacgaact
     6481 gccgccgcag tgggtccggg atcaactcgc cgtgcgcgca attttcatcc gattcgatgc
     6541 tataaagctt cgcgtccatg acacctgcgt ccgcgagtgc tttcaaaatg aacgacgccg
     6601 ctagaccgac aaagccaccc gattccacga caccgcgcgg ccgacgccag cgggtcagtg
     6661 catagagtgt ggcgcaatcc agtgcgccca tcagtcccgc gctgccatgc gcagcgcgtc
     6721 ggcgcatcat ctcggccatg aaatcgctcg cccgaatttc ggcggcaaat ccggcgatct
     6781 cgtcccatat accatagagc ggaatcggcc acggctcgag ttgtgacgcg cgttttctgc
     6841 gatgtcgcca gacgcgcgga agcagacgag tgcggcccat accgattgag agccgaacat
     6901 tcgctgccgg agttgctggc gcgaatgttt atttacagcg cgcgatcggc gagtgcgagg
     6961 atgcgacgtt tcttgtccgc gtaatcgagc gagcgaaagg cgcggatctc ttccaagcgc
     7021 cgaacaccgt tcagtgaatc gagaaactgc gtgtagctcg ctgctaactc ggttgtttct
     7081 aaataacggc cgcgcaaatt ttcatcgcga aacgcgtcgg gacattgccg ggcaaacaaa
     7141 tgaaaccgga gccagcgacg atcagagcgc gagacgcgct gtgttttccg gaagaacgcg
     7201 ataaacaaca ggaggacgag ataagtatcc acctgcgcct ggagctcgag attgcgcgcg
     7261 aaatcctccg acgcgatctc gcgcgccccg ttcctgaact ggagcgcagc gtgcaacgcg
     7321 tggttgatct cttccacaaa aacaatgagc gaacggatgt tgtggtcgcc caggccggcg
     7381 cgcggatcgt ggcgttcaag ttcttcgatc agccagcgcg aataatagat gccgacgtaa
     7441 agacgatcgt ccgtatgccg caaaaatgta cgagctagtt cgctcaactc ccgcgcggag
     7501 gttcccgcta agatcgacaa ttgcttgcag cgctcgcgat cgatcaggca ctcctcgaga
     7561 ttgatgccga cctgcgcgta ggtgcgctcg agcagctttt ggatttggct gaagagcgtc
     7621 tcgttcacag cgccagcgat ggccgcacgt cgtcgagatt gagcaagcga tccgacagtt
     7681 cgttcagtgc gagccgcaca tcgcgaaatc gctcggccaa gcgttcgaat acgtcgtcga
     7741 gttcgcagcg gcgcgcggtc tcgtgtgaag caaccagttg gtaatttgtc cggccgatct
     7801 gctcgtaaaa cttcaggtca ggtccgccgc gctcatggcg ccgttgcgtg ctctcgtgaa
     7861 aaatgccgct gataaagagt gaatagttcc cgatgtgggc gcgtaagaga aaggcctgct
     7921 cgggcgtgac gcgctgaagc gcgatgagca tgtcgcagat gtattcatgc gcatgattgc
     7981 caatgtccgc gggccgttcg agctgactgg cacgggaaaa tgtttcaagc agcgaagcga
     8041 cgtaatcgca cagtttgcgg tcgttgatcg ccgcctggcg caagacatgg cgcgccagca
     8101 cataaaaata aaactggggc gaaatgcgca actgcatcgg atgcgatagg atcgcgtcga
     8161 tcagccgcgg attatcgagg acggaatcgc gcgcctcgcc gtcgctcaaa agatcgacaa
     8221 gcgagacgtg attgccctgc gaacgcgcga gcgtgcggac gatgaaatca aaatccgcgg
     8281 cggtgaatcg ttcccgacaa ttcgccctga tcatcgacta tcctccacga ttttagagca
     8341 gtgacggtgc caaaggcaag cttgttcaca gggggagaaa gaaagcgccc aacgcttaac
     8401 ttcgaacatc caatatggaa atgagtttgc attcagaact gaatgttgga cgttcggcgt
     8461 tcgaagtttt cttcagcaga aggaagggtg gctgagtccg gtttaaggca ctcgactcga
     8521 aatcgagcgt gggcttgaaa cccaccgtgg gttcgaatcc caccccttcc gctggct
//
LOCUS       DASTDG010000006         8123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_635915, whole genome shotgun sequence.
ACCESSION   DASTDG010000006 DASTDG010000000
VERSION     DASTDG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635915"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VFO30_00175"
     CDS             <1..105
                     /locus_tag="VFO30_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002144919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9417729.1"
                     /translation="ELMNQADIDGALVGGASLDPHSFAQIVNAAREEV"
     gene            complement(157..729)
                     /locus_tag="VFO30_00180"
     CDS             complement(157..729)
                     /locus_tag="VFO30_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET9417730.1"
                     /translation="MLEAQQQLDLAGQLRQSELVVPFHITQTGPLIRYSFSNPEEVLQ
                     LRLGEHSSRLDLVTDTAAEKFTASKLNQKIRGTGITYEDLALKFLYWPDARLVGQENV
                     RTRNCWKLQLPAPSRESQYSNVLLWVDKGSGALMRMEGYDWNGQLAKRFEVVSAQKID
                     NRWFLKQMRVEELQPGTSRVLSRTYLEIKK"
     gene            908..1558
                     /locus_tag="VFO30_00185"
     CDS             908..1558
                     /locus_tag="VFO30_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9417731.1"
                     /translation="MALDESIFHLINEHWTNPALDLFMAALSDLDIWQPVLSAVLIFI
                     LVFGGFKGRACVFCVLITLLVAEQIIGALKWAVDRHRPKQVESVRLVELQRSRPALMT
                     LFKKPVVRFSDASDRKRSGNSFPSGHMMDNTAVALCLMLCYRRGWLYWIVTALIGYSR
                     VYLGAHWPSDIVATFFLAVGETLLVLAGLELAWRWAASKWAPQLYLRHPRLLTELR"
     gene            1558..3135
                     /locus_tag="VFO30_00190"
     CDS             1558..3135
                     /locus_tag="VFO30_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9417732.1"
                     /translation="MSTTRAAWLFTGLLTAVRLSILPTTDLEFDEAHYWLWSQHLAPA
                     YFSKGPAIAFVIRASTALFGSNEFGVRFFSPILAAGTSLLLFYFARRLFSAAAALWSV
                     LALNVTPLFNVGAFLMTIDPLSIFFWLAAMFSFWIAVEKSPSWSWHWPLTGLLVGLGF
                     LSKYTNALELVSIILVLALVPRLRHEFARPRLYLLLTAFAVCTIPPIVWNAQHVWITL
                     THLKARGSLEQGFGIHPLEVLAFVGEHFLAYSPLLFLGLAWGVLGSCRRIGQQLKVLF
                     LFWFGLPVFAFYFLLSFNKVAAPNWDALAMLGFGLLAVHFWRERIAGHVSLRVVAAAA
                     LVVGLMMSALTLDTDMIRSLGLEFWRSDPSNRMRGWKSATLAVENIRNDIESKLGERL
                     FLIADARDRASEISFYLRDKKLEAPGHPPVYIVEAQDIQNQFSFWPRYDEFVEAKPGA
                     PRSDEEAATEGSAVNPFVGRNALFVREGFGERLPHNIRAGFQSVAPVAVIEVWRYGRL
                     LRRWQLFLCRAYRTLPL"
     gene            3132..3839
                     /locus_tag="VFO30_00195"
     CDS             3132..3839
                     /locus_tag="VFO30_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9417733.1"
                     /translation="MTGAGSPAVSVIVPVFNEQENIPILQSELRAALDGRDYEIVFVD
                     DGSTDRTVERIEPAPNVRLLRFEKNTGQSAAIYAGLQAARGTVAVLIDGDLQNDPADI
                     PRLLEEISRGADLVCGYRTERKDTLVKRVTSRIANFVRSRFTRDYVRDTGCTLKAMRR
                     QCIAALVPFKGMHRFIPALVRAAGFRLVEVPVHHRARRFGQSKYGLTNRAVRATVDMF
                     GVRWLLSRRLNYKIRES"
     gene            3914..4378
                     /locus_tag="VFO30_00200"
     CDS             3914..4378
                     /locus_tag="VFO30_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9417734.1"
                     /translation="MSLADLLSAEQIIPEMQATERWPAIVELIDLLLSLGKIKPPDRD
                     SILASLKQREETMSTGIGFGIAIPHCSSDRIDDVVAAFGRSTSGIEFDALDNAPVKFV
                     VLFIVPKNQFQVHLRTLASIAKFLNDRAVRDQLAAANSREEIVSIFRQRSPK"
     gene            4454..6169
                     /gene="argS"
                     /locus_tag="VFO30_00205"
     CDS             4454..6169
                     /gene="argS"
                     /locus_tag="VFO30_00205"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9417735.1"
                     /translation="METFQSLLQKRLSDALIKTGFSEAGELTTTTDPRFGDYQSTAAL
                     VLGKQRGENPREIGAKIVAHLNLGDWAEPPQIAGAGFINFRLRPTSIAKQAAGVLRDE
                     RLGVATSTSPLKIVIDFGSPNVAKPMHVGHIRSTVLGDALARIAQFLGHDVIRDNHIG
                     DWGTQFGMVIWGWKNLLDRQSLERDPLSEIVRIYKETNLRSTTDPQVREACRQELVKL
                     QAGDAGNLDIWKQCVAFSMRDFERVYELLDIRYDIQCGESFYNDRLAGVVERLLKKGI
                     AQMSEGAVVVFFPEIPELHDKPCIIRKRDGGYNYATTDIATVEYRVKDLKRDTLWYVV
                     GAPQILHFKQIFAIAGRAGYAADFRHITFGSILGEDRKLMKTRAGDNVPLRDVLEEAC
                     RRAREIVEEKNPELSEQDKIEIGRIIGIGAVKYAELSQYRMTDYVFAWDKMLSLQGNT
                     APYLQNAYVRTQSIFRKAGRARSPSTKIDNLMLADPAEIALAKKLCQFAEIVPQVLND
                     FRPNILASYLFDVANSFHGFYEACPVLKSAEPARSSRLALCDLTAHVLQRGLDLLGIK
                     VPGKM"
     gene            6267..>8123
                     /gene="treY"
                     /locus_tag="VFO30_00210"
     CDS             6267..>8123
                     /gene="treY"
                     /locus_tag="VFO30_00210"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET9417736.1"
                     /translation="MRDSSTSLGMTKGENHLRIPSCTYRLQFNRRFTFRQARDIVPYL
                     HALGVSDVYASPYFQASPDSLHGYDITDHNQLNEAIGSRAEYDGFIAELHAHGMGQVL
                     DFVPNHMGIGHHNQWWMDVLENGPSSVYAKYFDIDWRPIKTDLHDKVLLPVLGDQYGR
                     VLERGELKVHFEAGAFYLTYYEHQFPIAPGTYRYILDVALEKLIDHKTEDFYAELQSI
                     RTALEYLPRRTETDREKIAERAREKEIIKRRLERRCQEAPQVEHAIEQAVDEINGRSG
                     DARSFDRLDELLNAQAYRLAFWRVAADEINYRRFFDINNLAAVRQEEPEVFEATHRLL
                     LELIANGAVTGLRVDHPDGLYLPRPYLEKLQQRCAHALGNTAADRRAIFMLVEKILTG
                     EEQLRSDWPVHGTTGYDFASDVTQLLVDQAAEAAITNTFRKFIGHSLHYRHLVYAKKR
                     LVMRLALANDVNVLGNMVDRLSEQNRWFRDFTLQALVVAVRETIACFPVYRTYVEAAR
                     SVGEQDRDIIQRAIAAAKRRNPGIETSVFNFLRDILLLQFPENLDATAREEHTHFVLK
                     FQQSTAPIMAKGVEDTVFYIFNRLAALNEVGGEPEQFGSGIEPFHRANQDRHR"
BASE COUNT         1645 a         2352 c         2331 g         1795 t
ORIGIN      
        1 gagctcatga accaggccga tattgacggt gccctcgttg gcggcgccag tctcgacccg
       61 cacagcttcg cgcaaatcgt gaatgcggcg cgcgaagaag tgtagcacgc gtctgtggcg
      121 cgtctcacgt gacggcccgc agggccgtgg ctacagttac ttctttattt cgagataggt
      181 ccgcgacagt acccgactgg tgccgggctg cagttcctcg acgcgcattt gtttcaggaa
      241 ccagcgattg tcgatctttt gcgcggagac gacttcgaag cgtttcgcga gctgcccatt
      301 ccaatcgtag ccttccattc gcatgagcgc gccgctcccc ttgtccaccc agagcaggac
      361 atttgaatac tgcgactctc gcgagggcgc cggcagttgc agtttccagc agttgcgcgt
      421 gcggacgttt tcctgcccga cgaggcgcgc atccggccag tagagaaatt tcagcgcgag
      481 atcttcgtag gtaattccgg tgccgcggat tttttggttc aacttcgacg cggtgaactt
      541 ttccgcggcg gtgtcggtga cgagatccag tcgcgagctg tgttcgccca ggcgcagctg
      601 cagaacttct tcgggattcg aaaacgagta gcgaatcagc ggaccggttt gggtgatgtg
      661 aaaaggaacc acgagttcgc tttgtcgcag ttgacccgcg agatcgagct gctgttgtgc
      721 ctcgagcatg cggacagaat cgaggatctc tttagcgctc gggcgagctg gctgtgcttg
      781 aagcgacaac gcaccaataa acaacgcgct gaatgttttt cgtatcacgg cggactttag
      841 cgcttaagtg ttaagtttta agtagaaggc ggaaggctta aaacttaaga cttaaaagtt
      901 ttcaactttg gctttggacg aatcgatttt tcatctgatc aacgaacact ggacgaatcc
      961 cgcgctggat ttgttcatgg ccgcgctgag cgatctcgac atctggcagc cggtgctcag
     1021 tgccgtccta attttcatcc tcgtattcgg cggcttcaag gggcgcgcct gtgtcttctg
     1081 cgtgctcatc actttgcttg tcgcagaaca aattatcggc gctttgaaat gggcggtgga
     1141 tcgacatcgc ccaaagcaag ttgagagcgt tcgcctcgtt gagctacaac gaagccgtcc
     1201 tgctttgatg accttgttca aaaaaccggt cgtccgtttc tcggatgcaa gcgatcggaa
     1261 acgatcgggc aattcattcc cgtccgggca catgatggac aacactgcgg tcgcgctctg
     1321 cctcatgtta tgttatcggc gcggttggct ctactggatc gtcactgcgc tgatcggtta
     1381 ttcgcgggta tatctcggcg cgcactggcc aagcgacatc gtcgcgacgt tctttcttgc
     1441 ggtcggagaa acactgcttg tcctcgccgg cctggaactg gcgtggcgat gggctgcctc
     1501 aaaatgggcg ccgcaattat atttgcgcca tccgcgttta ctcaccgagc taaggtaatg
     1561 agcacgacgc gcgcagcctg gctctttact ggccttctca ccgccgtccg attgtcaatc
     1621 ttgccgacga ccgatctcga attcgatgag gcgcattact ggctctggtc gcagcatctt
     1681 gcaccggcgt atttcagcaa aggcccggcc attgctttcg tgattcgggc gagcacggcg
     1741 cttttcggct cgaacgaatt tggcgttcgt ttcttcagcc cgatcctcgc cgccggaaca
     1801 agcctgcttc ttttttattt cgcacgccga ctcttcagcg cggcggcggc cttgtggtca
     1861 gttctcgcgc tcaacgtcac tccacttttc aatgtgggcg cgttcctgat gacaatcgac
     1921 ccattatcga tcttcttttg gttggcggcg atgttcagct tctggatcgc agtcgaaaaa
     1981 agcccctcct ggtcatggca ttggccgttg acgggtttgc tcgtcggcct gggttttctg
     2041 tccaaataca ccaacgcgct ggagctggtt tcgatcattc tcgtgctcgc gctcgtgccg
     2101 cggttgcgtc acgaatttgc gcggccgcga ctctacctgc tgcttactgc ctttgccgtc
     2161 tgcacgattc cgccaatcgt ctggaacgcg cagcacgtct ggataacgct gacgcacctg
     2221 aaggctcgcg ggagcctcga acaaggcttc ggcattcatc cgctcgaagt cctcgccttc
     2281 gtcggagagc actttctcgc ctattcgccg cttcttttcc tcggtctagc gtggggagtg
     2341 cttggcagct gccggcgcat tggccagcaa ctgaaagtgc tgtttttgtt ctggttcggc
     2401 ctgccggtct tcgcatttta ctttctcctc tccttcaaca aagtggcagc gccgaattgg
     2461 gacgcactgg ccatgctcgg cttcggttta ctcgccgttc acttctggcg agagcggatt
     2521 gccggccacg tctcattgcg agttgtggcc gcggcagcac ttgtggtcgg cctgatgatg
     2581 agcgccctca ccctcgatac cgacatgatt cgcagcctcg gcttggagtt ttggcggagc
     2641 gacccgagca atcgcatgcg cggctggaaa tcggcgacgc tcgcggtcga gaacattcgc
     2701 aacgacatcg agtcgaaact gggtgagcgc ctcttcctca tcgccgacgc acgcgatcgc
     2761 gcttccgaga tctcttttta tctgcgggac aaaaaactcg aagctccggg tcatccgcct
     2821 gtttacatcg ttgaggcgca ggacattcag aaccagtttt cgttttggcc gcgctatgat
     2881 gaattcgttg aagccaaacc gggagcgccg cgatcggatg aagaggccgc caccgagggc
     2941 agcgcggtca atccatttgt cggccgcaac gcgctttttg tgcgtgaagg atttggcgaa
     3001 cgactcccgc acaacattcg cgccggcttt caatcggttg cgcccgtcgc tgtgattgag
     3061 gtttggcgtt atggccgttt gctccgccgg tggcagttgt tcctctgtcg agcttatcgc
     3121 acgctgccgc tatgactggc gctggatcgc ccgccgtttc agtgatcgtg ccagtgttca
     3181 acgaacaaga gaacataccg atcctgcaat cagagctgcg cgccgccctt gacggccgcg
     3241 actacgaaat cgtctttgta gatgatggct cgactgatcg caccgtcgag cgaatcgaac
     3301 cggcgcccaa tgtgcgtctc ctccgcttcg aaaaaaatac cggacaaagt gccgcgatct
     3361 acgcgggttt gcaggcggcg cgcggcactg tcgccgttct catcgacggc gatttgcaaa
     3421 acgacccggc cgatattccg cggctgctcg aggaaatttc gcgcggggcc gatctcgtct
     3481 gcggttaccg tacggagcgg aaagatacgc tggtcaagcg cgtgacaagc cgcatcgcaa
     3541 acttcgtgcg cagtcgattt accagggatt acgtgcgcga taccggttgc accctcaaag
     3601 cgatgcgccg ccaatgcatc gccgcgcttg ttccgttcaa ggggatgcac cgtttcattc
     3661 cggcgctggt ccgcgccgcg ggttttcgcc tcgtcgaagt tccggtgcat catcgcgcgc
     3721 gtcgcttcgg ccaaagcaaa tatggtctca ccaatcgcgc cgtccgcgcg accgtggaca
     3781 tgttcggcgt gcgctggctg ctctcacgac gattgaacta caaaatccgc gagtcttgac
     3841 cggggcgccg aaaacgcgct tgtcaaccta accaccgacg ctaagcttgc gctccccatg
     3901 cggggaacgc tgcatgtcgc tggccgattt actttctgca gagcagatca ttcccgagat
     3961 gcaggcgacc gagcgttggc cggcgatcgt ggaattgatc gacttgctcc tgagtcttgg
     4021 caaaatcaaa ccgccggatc gcgatagcat cctcgcgtcg ttgaaacagc gcgaagaaac
     4081 gatgagcacc ggcatcggtt ttgggatcgc gatcccgcat tgttcgtccg accggatcga
     4141 tgacgtggtc gcggcttttg gaagatcgac aagcggcatt gagttcgacg cgctcgacaa
     4201 tgcgccggtg aagtttgtcg tcctgttcat cgtgcccaag aaccagtttc aggtgcatct
     4261 ccggacgctc gcatccatcg ccaagttttt gaatgaccgc gcggtgcgcg accagctcgc
     4321 agccgccaac tcgcgagaag agatcgtgtc gatcttccgc cagcgttcgc cgaaataacg
     4381 gtggccgccc cgctcagtcg gggcgaatta tttcaaatga gaacgcggcg acaaaacgcc
     4441 gcggctacag ctgatggaga catttcagtc gctgctccag aaaagactgt cggacgccct
     4501 gataaagacc ggcttttccg aagccggcga gctaacgacg acaaccgatc cacggtttgg
     4561 cgattaccaa agcaccgccg ccctcgtcct cggcaaacag cgcggtgaaa acccacgcga
     4621 aattggggcg aaaattgtgg cgcatctgaa cctcggcgac tgggccgagc cgccacagat
     4681 tgccggcgcc ggcttcatca atttcagatt gcggccgact tctatcgcaa agcaggccgc
     4741 cggcgtcctg cgcgatgagc gactcggcgt cgcaacatcg acatctccgc tcaagatcgt
     4801 gatcgacttt ggctcgccca acgtggccaa gccgatgcat gttggccaca tccgcagcac
     4861 tgtgttgggc gacgcgctgg cgcggatcgc gcagtttctc ggtcacgacg tgatccgcga
     4921 caatcacatt ggcgattggg gaacccagtt cggcatggtg atctggggtt ggaagaacct
     4981 cctggatcga caatcgctcg agcgcgatcc gctgagcgag attgtgcgga tttacaagga
     5041 gaccaatctc cgctccacaa ccgatccgca ggtgcgcgag gcgtgccggc aggagctggt
     5101 gaagctccaa gccggtgacg cagggaatct ggacatttgg aagcaatgcg tcgcgttttc
     5161 gatgcgggat ttcgagcgcg tctatgaatt gcttgatatc cgctacgaca tccagtgcgg
     5221 cgaaagtttt tacaacgatc ggctcgctgg cgtggtcgag cgattgctca aaaaaggcat
     5281 cgcgcaaatg agcgaaggcg cggtcgtcgt tttctttccc gaaattccag aattgcacga
     5341 caagccgtgc atcatccgca agcgcgacgg cggctataac tacgcgacga ccgacatcgc
     5401 gacggttgag tatcgtgtga aagatttgaa acgcgacacg ctctggtacg tggttggcgc
     5461 gccgcagatt ctgcatttca aacaaatttt cgccatcgca ggacgcgcgg gttacgcggc
     5521 ggattttcgc cacattacct tcggcagcat cctcggcgaa gaccgcaagc tgatgaaaac
     5581 gcgcgccggc gacaacgtgc cgttgcgcga cgtgctcgag gaagcgtgcc gccgcgcgcg
     5641 ggagatcgtc gaggaaaaaa atcccgagct gagtgagcag gataagatcg agattgggcg
     5701 gatcatcggg atcggcgcgg tcaagtacgc cgagctttcg cagtaccgga tgaccgatta
     5761 cgttttcgcg tgggacaaaa tgctgtccct acagggcaac accgctcctt atttgcaaaa
     5821 cgcttacgtc cgcactcagt cgatttttcg caaagcaggc cgggcaagat ctccatctac
     5881 gaagatcgac aatttaatgt tagcggaccc ggccgagata gctctcgcga agaaattgtg
     5941 tcagttcgcg gaaattgttc cgcaagtact gaatgatttt cggccgaaca ttctggccag
     6001 ttatttgttc gacgtggcga acagtttcca tggcttctac gaagcctgcc cggtcttgaa
     6061 gtcggcagag ccggcgcgca gctcgcgcct ggcgttatgc gacctcaccg cgcacgtctt
     6121 gcaacgcgga cttgatttgt taggcatcaa agtgcctgga aaaatgtgag tcgcttgtca
     6181 tgtcgagcgt agtcgagaca tctcttatta ctaagttcta cacctggctt catctcgggg
     6241 tagatgtgct tcaaggcaga taaacaatga gagattcctc gacttcgctc ggaatgacaa
     6301 agggcgagaa ccaccttcgt atcccgagct gcacctatcg gctgcagttc aaccgccggt
     6361 tcacattcag gcaggcgcgc gacatcgtgc cttatctgca cgcgctcggc gtgagcgatg
     6421 tctacgcgtc gccttatttc caggcgagcc ccgatagcct gcacggctac gacattaccg
     6481 atcataacca gctgaatgaa gccatcgggt cgcgcgcaga gtacgacggc ttcatcgcgg
     6541 agttgcacgc gcacggcatg ggacaggtgc tcgatttcgt ccccaatcac atgggcatcg
     6601 gccatcataa ccaatggtgg atggacgtgc tcgaaaacgg gccaagctcc gtctacgcga
     6661 aatatttcga catcgactgg cggccgatca aaaccgacct gcatgataag gttctgctgc
     6721 cggtgctggg cgaccaatac ggacgggtcc tcgaacgcgg cgagctcaag gtgcacttcg
     6781 aagccggcgc gttttatttg acctactacg agcatcagtt cccaatcgcc cctggcacgt
     6841 atcgttacat ccttgatgtc gcgctcgaga agttaatcga tcacaagacc gaagactttt
     6901 acgcagaact acaaagcatt cgcaccgcgc tcgagtatct gccgcgccgc accgaaaccg
     6961 accgggagaa aatcgcggag cgcgcgcggg aaaaggaaat tatcaagcgc cggctcgagc
     7021 gtcgctgtca ggaagccccg caagtagagc acgcgatcga gcaggcggtg gacgaaatca
     7081 acggccgctc gggcgatgcg cgcagcttcg atcgactcga cgagttgctc aacgcgcagg
     7141 cttaccggct ggctttctgg cgcgtcgccg cggatgaaat caactaccgt cgctttttcg
     7201 acatcaataa cttggccgcc gtgcgtcagg aagaaccgga agtattcgag gcgacgcatc
     7261 ggcttttact cgagctcatc gcgaacggcg ccgtgactgg actgcgagtt gatcacccgg
     7321 acggtctcta cctgccgcgc ccgtatctcg aaaaattaca gcaacgctgc gcgcacgcgc
     7381 tgggcaatac tgcggcggac cgccgcgcga ttttcatgct cgtggaaaaa atcctgacgg
     7441 gcgaggagca attgcgcagc gactggccgg tgcacggaac caccggctac gacttcgcca
     7501 gtgacgtgac gcagctactg gtcgatcaag cggcggaggc ggcaatcaca aatacttttc
     7561 gaaaattcat cggtcattcg ctgcattacc ggcatcttgt ttacgcgaag aagcgcctgg
     7621 tcatgcggct ggcgctggcc aacgacgtca acgtgttggg caatatggtg gaccggttat
     7681 cggagcaaaa ccgctggttt cgggatttca ccttgcaggc gctggttgtg gcggtgcggg
     7741 aaacgattgc ctgttttccg gtttaccgga cctatgtcga agctgcgcgg tccgtaggtg
     7801 aacaagatcg cgacatcatt cagcgcgcca ttgccgcggc caagcggcgt aaccccggca
     7861 tcgaaacgtc cgttttcaat tttctacggg acattttgct gttgcaattc ccggagaacc
     7921 tggacgcaac agcgcgcgag gaacacaccc acttcgtttt gaagtttcag caatccaccg
     7981 cccccatcat ggccaaaggc gttgaggaca cggtctttta cattttcaat cgtctcgctg
     8041 cactgaacga agtaggcggc gagccagagc aattcggctc cgggatcgag ccattccacc
     8101 gtgcgaacca agatcgacat cgt
//
LOCUS       DASTDG010000007         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_518709, whole genome shotgun sequence.
ACCESSION   DASTDG010000007 DASTDG010000000
VERSION     DASTDG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518709"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFO30_00215"
     CDS             <1..697
                     /locus_tag="VFO30_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9417737.1"
                     /translation="KFGREHRDQTAAKLGPLADSVLKILNDGGTSEKLERLVAGLRER
                     HLADFVQVDVRIVRGLAYYTGIVFEVFDRAGKFRAIAGGGRYDNLIAQLSDGAVSMPA
                     LGFAMGDVVLGELIDKIPRAREAMQSAIRAKRKIDIYIVIAKEARRSDALSHIQQLRE
                     RGYRVDYPLVAEKVGKQFQAAEASGAGIALLYGDEWPQVKVKNLATREESLIANDALL
                     DSVAKFFNVPGSS"
     gene            694..2499
                     /gene="aspS"
                     /locus_tag="VFO30_00220"
     CDS             694..2499
                     /gene="aspS"
                     /locus_tag="VFO30_00220"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9417738.1"
                     /translation="MSTYRDTDCNALRKGDIGREVTLAGWVHVSRDHGGVIFIDLRDR
                     EGITQVVFRPEENAQLAKAAHTLRSEDVIQITGRVAARVPGTENPKLATGDIEVIPNR
                     LTILNRADDLPFPIDAEIHNEDLRLTYRYYDLRRPELSRNLRVRHRVAKATRDFLDSQ
                     GYLEIETPILSKSTPEGARDFLVPSRLMPGKFYALPQAPQQYKQLLQVAGVEKYFQIA
                     KCFRDEDLRADRQPEFTQIDIEASFVRPDDIFALTEGMLAAVFNAAFKIDIKTPFDRL
                     TYFEAISRYGSDKPDRRFGVELVDLSDDFRESSFKVFRSALEAGGVVKAINAKGFAGI
                     TTGQIEELTETAKVFGAKGLAFIKVENGEWKSPIVKFFSDAEKAALQSKLRIEEGDLI
                     LFGADKWDIACEVLGRVRLRVAEILNLVRSSANRPSGSDSASDWDFLWVTDFPLMQWS
                     AEENKWNAVHHPFTRPKPEDMHLLEQKKFADIRAEAYDVVLNGVEIGGGSMRIHEPQL
                     QEQMFEALGISAQDRQSQFGHLLRALQLGAPPHGGIALGLDRLVMLLCGAESIRDVMA
                     FPKNNRGMDLMTQSPAEVDPRQLRDLSIKLDGEKK"
     gene            2496..2693
                     /locus_tag="VFO30_00225"
     CDS             2496..2693
                     /locus_tag="VFO30_00225"
                     /inference="COORDINATES: protein motif:HMM:NF040165.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417739.1"
                     /translation="MSEELHPEQIKALRKMTPERRLETGLAFMEEMRQLKAAALRAQH
                     PDWTEEQIARALREFILHGAS"
     gene            2680..3237
                     /locus_tag="VFO30_00230"
     CDS             2680..3237
                     /locus_tag="VFO30_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417740.1"
                     /translation="MALPKVAATIADRLNATGVTWMATGAIAAITYGDYRVTNDIDVI
                     LVLASEDIQKLTAAFPLEEFYCPPADVIAMEAARESHGHFNLIQHETGFKADIYIATN
                     EPLTQWALQRRRKLQLADTTMWVAPPEYVIIGKLDFFREGGSEKHLRDIRGMLAGTEI
                     DRSFLEKEIAQRGLQEAWRAVQKRS"
     gene            complement(3239..3424)
                     /locus_tag="VFO30_00235"
     CDS             complement(3239..3424)
                     /locus_tag="VFO30_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417741.1"
                     /translation="MVSLVERILELHKELAAARSEHAQTNLQRQIDASDAQIDQLVYE
                     LYDLTPEEIKIVQSAAT"
     gene            complement(3444..3689)
                     /locus_tag="VFO30_00240"
     CDS             complement(3444..3689)
                     /locus_tag="VFO30_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417742.1"
                     /translation="MPAPRVIGDLVERFEQQGDAYKSGQYNEAQLRKEFVDPMFKALG
                     WDMDNISGYAKAYKDVIHEDAIKIGARPKRPTTHRQS"
BASE COUNT          864 a         1073 c         1177 g          797 t
ORIGIN      
        1 taagttcgga cgcgaacatc gcgaccagac cgccgcgaaa cttgggccgc tggccgactc
       61 cgttttgaaa attttaaacg atggcggcac gagcgaaaaa ctggagcggc tcgtcgcagg
      121 tctgcgcgaa cgccatctgg cggatttcgt tcaggtcgac gtgcggattg tgcgcggtct
      181 ggcctactac accgggatcg ttttcgaagt gttcgatcgc gcgggaaaat ttcgggccat
      241 cgcgggcggc ggacgttacg acaatttgat cgcgcagttg agcgacggtg ccgtctcaat
      301 gccggcgctt ggctttgcca tgggtgatgt cgtgctcggt gaattgatcg acaaaatacc
      361 gcgtgcccgc gaagcgatgc agagcgctat tcgcgccaaa cgcaagatcg acatctacat
      421 cgtcatcgcc aaggaagcgc gtcgcagtga cgcgctttcg cacatccagc aactgcgcga
      481 gcgcggttac cgcgtcgatt acccgctcgt ggctgagaag gtcggaaaac aatttcaggc
      541 cgccgaagct tccggcgctg ggatcgcctt gctttacggt gatgaatggc cacaggtcaa
      601 ggtgaagaac ctcgccacgc gggaggaatc gctcatcgcg aatgacgcgt tgctggacag
      661 cgttgcgaaa ttcttcaacg tgcccggttc gtcatgagca cgtaccgcga cacggattgc
      721 aacgcactgc gcaaaggcga catcgggcgc gaggtcacgc tcgccggttg ggtgcacgtc
      781 tcgcgcgatc acggcggcgt gattttcatc gacctgcgtg accgcgaagg cattacccag
      841 gtggtgtttc gtccggagga aaacgcgcag ctcgcaaagg cggcgcacac acttcggagc
      901 gaagacgtga tccaaattac cgggcgcgtt gccgcgcgtg tgccgggcac ggaaaaccca
      961 aagctggcga caggcgacat cgaggtcatt ccaaaccgcc tgacgatctt gaatcgcgcg
     1021 gacgacctgc cgttcccgat cgacgcggag attcacaacg aagatttgcg gctcacctat
     1081 cgctattacg atctgcgtcg gcccgagctc tcacgcaatt tacgggttcg gcatcgcgtg
     1141 gcgaaagcga cgcgcgactt tctcgattca cagggatacc tggaaatcga aacgccgatt
     1201 ctgtccaaga gcacgccgga aggggcgcgc gattttctcg tcccgagtcg cctcatgccg
     1261 ggaaaatttt atgcgttgcc acaagcgccg cagcagtaca agcagctcct tcaggttgcg
     1321 ggcgtcgaaa aatattttca gatcgcgaag tgctttcggg acgaagattt gcgcgccgac
     1381 cgccaaccgg agttcacgca aatcgacatc gaagcgtcat tcgttaggcc ggacgacatt
     1441 ttcgcgctga cggaaggaat gctcgcagcg gttttcaacg cggcttttaa gatcgacatc
     1501 aaaacgccgt tcgatcggct gacgtatttc gaggcgatca gccggtacgg aagcgataaa
     1561 ccggaccgcc ggttcggcgt cgagcttgtc gatctgagtg atgattttcg cgagtcgagt
     1621 ttcaaagtgt ttcgcagcgc gctggaggcc ggtggcgtgg tcaaggcgat caatgccaaa
     1681 ggctttgccg gaatcacgac cggtcagatc gaagagttga ccgagacggc aaaagttttc
     1741 ggcgcaaaag gtctcgcttt tatcaaggtt gaaaacggcg aatggaaatc gccgatcgtg
     1801 aagtttttca gtgacgccga gaaagcggcg ctgcagtcaa agctaaggat agaagagggc
     1861 gatctcattt tgttcggcgc cgacaaatgg gacatcgcct gcgaagtgct gggtcgcgtt
     1921 cgcctgcgcg tcgcggagat cttgaatttg gttcgcagct ctgccaaccg cccgtccggc
     1981 tcggactccg cctcggactg ggattttctc tgggtaaccg atttcccgct catgcaatgg
     2041 agcgcggaag aaaacaaatg gaatgcggtg catcatccgt tcacgcgacc gaaaccggaa
     2101 gatatgcacc tgctcgagca aaagaaattc gccgatattc gcgccgaggc ttacgacgtt
     2161 gtgctcaacg gcgtggagat cggcggcggc agcatgcgga ttcacgagcc gcagttgcag
     2221 gaacaaatgt tcgaagcgct cgggatcagc gcgcaagatc gacaatcaca gttcggtcat
     2281 ttgctgcgcg cgctgcaact cggcgcaccg ccgcacggcg gcatcgcgct cgggctcgat
     2341 cgccttgtca tgctgctctg tggcgcggaa tcgatccgcg acgtgatggc cttccccaaa
     2401 aacaaccgcg gcatggattt gatgacccaa tcccccgccg aagtcgaccc gcgccagctc
     2461 cgcgacttgt cgatcaaact ggacggtgaa aagaaatgag cgaggagcta caccccgagc
     2521 aaatcaaagc gctgcgaaaa atgactccag agcgacgcct ggagactggc ctcgcattta
     2581 tggaggagat gcgacagctg aaggcggcag ctctgcgcgc gcagcatccg gattggactg
     2641 aagagcaaat cgcgcgtgcc ttgcgcgaat tcatattgca tggcgcttcc taaagttgca
     2701 gcgacaattg cagaccggct gaatgcaact ggagtaacct ggatggcaac cggcgcgatc
     2761 gccgcgatca cctacggtga ctatcgggtc acaaacgaca tcgatgtgat tttggttctt
     2821 gccagcgagg acattcaaaa gctgactgcg gcgtttccgc tcgaggagtt ttattgtccg
     2881 cctgcggacg taattgcgat ggaagcagca cgcgaatcgc acgggcattt caatctgatt
     2941 cagcatgaga ccggttttaa ggccgatatt tacatcgcaa ccaatgaacc gctgacgcaa
     3001 tgggctttgc agcgacggcg taaacttcaa ctggccgata cgacgatgtg ggttgctccg
     3061 ccggaatacg tgatcatcgg taaattggat ttctttcgcg aaggcgggag cgagaagcat
     3121 cttcgtgaca ttcgcggcat gctggccgga acggagatcg accgcagttt cctggaaaag
     3181 gaaatcgcac agcgtgggtt gcaagaggcg tggcgcgctg tccaaaagcg gagctgagct
     3241 aggtcgctgc gctctggacg attttgattt cctccggcgt caggtcgtaa agctcgtaaa
     3301 ccagctggtc gatctgcgcg tcgctcgcat cgatctggcg ctggagattt gtttgggcgt
     3361 gctcgctcct ggcggcggcg agttctttgt gcaattccag gatccgctcg accagcgaga
     3421 ccattcgatc gtgtcgatct ttgtcagctt tgtcggtggg tagtcgggcg ctttggtcgc
     3481 gcccctatct tgatcgcgtc ttcatgaatg acatctttgt aggctttggc ataaccgctg
     3541 atgttgtcca tgtcccagcc gagcgctttg aacatcgggt cgacgaactc ctttcgcagc
     3601 tgggcctcgt tgtattggcc cgacttgtag gcgtcgcctt gttgctcgaa acgctcaaca
     3661 aggtcaccga tgacgcgagg agcaggcatt gacggcaaaa ctaaacgtcc aacgctcagc
     3721 gtccaacgct cagcttcgaa cgattgcgcg tgaggacacg atgcccctac accggcggct
     3781 gatgctcgcg gtcagtccag aaaagaatcg ccacgcaaaa ggtggcaatc ggcgcgggaa
     3841 aagaaatgag agagaacgga ttacacccgg tgcaaaatcc cgagcatggg ctgatcactt
     3901 aataacccag g
//
LOCUS       DASTDG010000008         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1306546, whole genome shotgun sequence.
ACCESSION   DASTDG010000008 DASTDG010000000
VERSION     DASTDG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306546"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..479
                     /locus_tag="VFO30_00245"
     CDS             99..479
                     /locus_tag="VFO30_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HET9417743.1"
                     /translation="MSSLSERMARLGIRDVDLEEIFARAGGPGGQHVNKVATAVTLRH
                     RPSGLSVTVQDSRSQAQNRKLARERLLDQIEDRQRTERQKEIAAREKERRRKSPRPTG
                     LKQRILETKRRRAKLKRDRARVVD"
     gene            554..1624
                     /gene="pilM"
                     /locus_tag="VFO30_00250"
     CDS             554..1624
                     /gene="pilM"
                     /locus_tag="VFO30_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET9417744.1"
                     /translation="MARRADSVVGIDLGKHAFKAVSLRRKGDARLVLTNFASHVVPEE
                     FGSADELAHELKGLLRELGGSAKACAVAVSDPNALLRIIEQPDTPIELLRSALRLNGL
                     VVLNQECKDFVLDVAPVNVNGSRNAPGETAAAPRSAATGVMTQTKYLVGGMLREKVKR
                     ISQALAKTHVSTQILQLAPVCSFNAFEVAYPEIFHHEAFMLFDIGHLQTTVLAGSKGE
                     LVLVRRIDYGGKALMQALTADGAIEKDAAWTLFEQGDPGMTDICRTSLARLANEVRNS
                     IGFFEGQREESIHRLFVSGGLARLETILQTLSDELGLPCEIWDPLETCEVALPASKRE
                     TLPREFVSLNVACGAAFEYLRG"
     gene            1630..2292
                     /locus_tag="VFO30_00255"
     CDS             1630..2292
                     /locus_tag="VFO30_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417745.1"
                     /translation="MALHLNLLHEEILEQRQRQRDPLKLGLLALIGCGTLMFGYYSWN
                     AYRTLEIRSRLGAIERSWSKMEPSVTAAQKKVAELQTLTKTVRSLDDYIADRFFWAPL
                     LQKIAHCVAPNTQLTSLDGTLDEARGVSITIEGVAAGREPRAAAEDLRQMLSEQLGHD
                     YSEVKVEFKSLEDLDTVVNLGGTHMPLARYNLAIAFKPGAQAKPTPTPAQNRNRRKRD
                     SE"
     gene            2289..2855
                     /locus_tag="VFO30_00260"
     CDS             2289..2855
                     /locus_tag="VFO30_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417746.1"
                     /translation="MSQKLSKEQTKKLGLSIVGFIFLVYIYFTFFLGPLQRSRADMLK
                     KIADRQSKLDTAQEELHKAAKLEQNAKDAMVRFNALRKVNAEGAPLAWFPPRIKTFFA
                     NEQIDKAVARLEGSSELKQEELSGWLRYNWVIDLPQTDYTTLGRAIADLENSEPLLLL
                     TRLSIHAMPDQPQFQHVELAAANLIEKK"
     gene            2852..3262
                     /locus_tag="VFO30_00265"
     CDS             2852..3262
                     /locus_tag="VFO30_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417747.1"
                     /translation="MKILHFVLISVACAALNAFSAEERTPGIELKNKSSFQMDSNGRN
                     PFWPIGFKPAAPTARGSSAGLDIPPNALLLTSITLDQGTHFAIINGKTMQEGQQFTMQ
                     VGSQSYVLKLKWIQDGQVILSYQDREIAVPLRRK"
BASE COUNT          783 a         1011 c         1039 g          637 t
ORIGIN      
        1 cctcacccta ccctctccct gagggagagg cggacgtcga agccgccggt gagggttaat
       61 agctcgtctc gaccgatcgc aatgcgcgac tggtatcgat gagttctctc tccgaacgca
      121 tggcgcggct cggcattcgc gatgtcgatc ttgaggagat ttttgcgcgt gccggcgggc
      181 cgggtggtca gcatgtgaac aaggttgcga ccgcagtgac gctgcggcat cgaccgagcg
      241 gactcagcgt gaccgtgcag gactcgcgtt cccaggcgca gaaccggaag ctcgcgcgcg
      301 aacggttgct cgatcagatt gaagatcgac aacgtacgga gcggcaaaaa gaaatcgccg
      361 cgagggaaaa ggagcgccgt cgcaaatcgc cgcggccgac cgggttgaag cagcggattc
      421 tggaaacaaa acgccgacgc gctaagctga aacgagatcg cgcgcgggtc gttgattaga
      481 gatcgacgcg cctgtgtaga gccacaaaac cgcgcctgtc ggcacacttt ccgctacatg
      541 ggtcggcagc atcatggcga ggcgcgcaga ttcggtggtc ggcatagatt taggcaagca
      601 tgcgttcaaa gcggtgtcgc tgcggcgcaa aggcgatgcc cggcttgtcc taacgaattt
      661 cgcctcgcac gttgtgccgg aagaattcgg ctcagcggat gagctggcgc acgagctgaa
      721 gggattactg cgcgagctcg gcggcagcgc caaagcatgc gcagtggcgg tttccgatcc
      781 gaacgcgctc ttgcggatta tcgaacagcc agacacacca atcgagctgc tgcggagcgc
      841 gctgcgtttg aacggtctgg tcgtgctcaa ccaggagtgc aaagactttg tgctcgatgt
      901 cgcgccggta aatgtgaacg gcagcaggaa cgcgccgggt gaaacggccg cggcgccaag
      961 atccgccgcc accggcgtga tgacgcaaac caaatatctg gtcggcggga tgctgcgtga
     1021 gaaagtgaag cgaatctcac aagcgctggc caagacgcat gtctcaacgc aaatcctgca
     1081 gctcgcgccg gtctgttcgt tcaacgcctt tgaggtcgcc tatccggaga tttttcacca
     1141 cgaagccttc atgcttttcg atatcggcca tttgcaaacc acggtgctgg caggcagcaa
     1201 aggcgaactg gtgctggtac gcaggatcga ttacggcgga aaagctttga tgcaggcgct
     1261 cacggcggat ggcgccatcg aaaaggatgc cgcctggact ttgttcgagc agggcgatcc
     1321 cgggatgacc gatatctgtc ggacgtcact cgcccggctc gcgaatgaag tgcgcaactc
     1381 gattggcttt tttgagggac aacgcgagga aagcattcat cgcttgttcg tttccggcgg
     1441 tttggcgcgc cttgaaacca ttctccagac gttgagcgat gaactcgggc tgccgtgtga
     1501 aatttgggac ccgctcgaga cgtgcgaggt cgcgctgccg gcgtcgaaac gcgagacgtt
     1561 accgcgcgaa tttgtttcac tcaatgtcgc gtgcggcgca gcctttgaat atctccgagg
     1621 gtgaacggca tggcactcca tctcaacctc ctgcacgagg agattctgga acagcgccag
     1681 cgccagcgcg acccgctcaa actcggcctg ctcgcgctca tcggctgcgg cacgctcatg
     1741 ttcgggtatt actcctggaa cgcctaccgt acgcttgaga ttcggagccg cctcggcgct
     1801 atcgagcgaa gctggtcgaa aatggaaccg agcgttactg ccgcgcaaaa gaaagtcgcc
     1861 gaactgcaaa ccttaaccaa aacggtgcgc tcgctcgatg actacatcgc cgaccgattc
     1921 ttctgggcgc ccctactgca aaagattgcg cactgcgtcg cgccaaacac gcagctcacc
     1981 agcctcgacg gaacgttgga cgaggcgagg ggcgtgagca tcacgattga aggcgtcgcc
     2041 gccggccgcg aaccgcgcgc tgctgcggaa gatttgcgtc agatgctaag cgagcagttg
     2101 gggcacgact acagcgaggt gaaggtcgag tttaagtcgt tggaagatct cgacacggtg
     2161 gtcaatctcg gcggcacgca catgccgctg gcccgctaca atctggccat cgccttcaag
     2221 ccaggcgcgc aagcaaagcc gacaccgaca ccggcgcaaa accgcaaccg ccgcaagaga
     2281 gattcggaat gagccaaaag ctgagcaaag agcaaaccaa gaaactcggg ctgagcatcg
     2341 tcggcttcat ctttctggtg tacatctatt tcaccttttt tctcgggcca ttgcagcgca
     2401 gccgggccga catgctgaag aaaattgctg accggcaaag caagctcgac accgcccagg
     2461 aggaactgca caaagccgcc aagcttgagc agaacgcaaa ggacgcgatg gtgcgattca
     2521 acgcgcttcg caaagtcaat gcggaaggcg cgccgctggc gtggttccca ccgcggatca
     2581 aaacgttttt cgccaacgaa cagatcgaca aagcggtggc gcgcttggaa ggcagctccg
     2641 agttgaaaca agaggagttg tccggctggc tgcgatacaa ttgggtaatc gatctgccgc
     2701 agactgatta caccacgctt ggccgcgcca ttgccgattt ggaaaactcc gagccgctgc
     2761 tcctcctcac gcgcttgagc atccacgcca tgccggacca gccgcagttc cagcatgtcg
     2821 agctcgccgc cgccaatctg attgagaaga aatgaaaatc cttcacttcg ttttgatttc
     2881 agtcgcgtgc gctgcgctca acgcgttctc agccgaagag cgcacacccg gcattgagct
     2941 caagaacaaa tcttccttcc aaatggacag caatggccgc aacccattct ggccgatcgg
     3001 attcaagcca gcggcgccga cggcacgggg gagcagcgcc ggattggaca tcccaccgaa
     3061 cgcactgttg ctcacctcca ttacgctcga tcaagggacg cactttgcca ttatcaacgg
     3121 caaaaccatg caggaaggcc agcaattcac gatgcaggta ggtagccaga gttatgtgct
     3181 aaagctaaaa tggatccagg acggtcaggt cattctgagc taccaggacc gcgagatcgc
     3241 cgtacctctc cggcgcaaat aaactgcacc tcttagggcg cacatatgaa gcggcgctcg
     3301 atcacaggag ggcaggcagg cactcgaaga tcgacatttt ccgctgcgca cggcagttcc
     3361 tattgattta gcggccaaat ttgcggtgta atcaggcgtg gccgcaaacg gcgacgctat
     3421 atcggatgct gcattcgcca ccacgcactg gagcgtggtg ctcgcggcgc
//
LOCUS       DASTDG010000009         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_283730, whole genome shotgun sequence.
ACCESSION   DASTDG010000009 DASTDG010000000
VERSION     DASTDG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283730"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFO30_00270"
     CDS             complement(<1..230)
                     /locus_tag="VFO30_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9417748.1"
                     /translation="MKLASKLRELLGNDIVADDADTLAAHSGDKWFAAETPEVVVFAR
                     STRQVVELLKFASHERIPVTARGGGFGYVGGCV"
     gene            complement(374..679)
                     /locus_tag="VFO30_00275"
     CDS             complement(374..679)
                     /locus_tag="VFO30_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417749.1"
                     /translation="MIARIWHGWTKREDAQTYETMLRDEIFPGIAARKINGYRGAELF
                     INEDGEEVEFVTLLRFDSMAAVKEFAGTDEGKPVIYPKAEALLMRMDERSRHYRVAC"
     gene            complement(767..1576)
                     /locus_tag="VFO30_00280"
     CDS             complement(767..1576)
                     /locus_tag="VFO30_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET9417750.1"
                     /translation="MPSPRDRVLNEMTPQVLAGKTGVVFGVANKRSIAWAIAKAWAAA
                     GAKLIFNYQGERLKENVEELVGEFGEQTPFFPCDVSSDEEIKKFFEQVRGQTSVVDLL
                     LHSIAFAPKEALEGDFVSTTREAFRVAHDISAYSLVALAREVAPLMSNGGSIVSMTYY
                     GAEKVVPHYNVMGVAKASLEASTRYLAYDLGPKRIRVNCISAGPVQTLAARGISGFTQ
                     MLKHYQERAPLKRSCDPAELGTAGVFLASDAAAAITGQVIYVDGGYQIMGM"
     gene            complement(1549..2217)
                     /locus_tag="VFO30_00285"
     CDS             complement(1549..2217)
                     /locus_tag="VFO30_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HET9417751.1"
                     /translation="MSYLDQIDNDIAEKHLLKHPFYLAWARGELSREALTDYARQYYH
                     HIAAFPTYLSAVHSHCDHQSTRKQLLKNLIDEEAGSPNHPELWKRFTNALGIDNVDLA
                     NTPKQPETQHLIDTFRVVCGNGSTAEGLTALYAYESQIPAICESKIDGLKKHYGFTNP
                     EHYEYFSVHIEADREHSAVEREMLSRKIDSRNIEELRAAAARVLDALWQMLSGVCRRH
                     AIAC"
     gene            complement(2330..>2593)
                     /locus_tag="VFO30_00290"
     CDS             complement(2330..>2593)
                     /locus_tag="VFO30_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417752.1"
                     /translation="AMRSEDVPFLERWADLWYRHVSSVFLQSYLRTTAGAVFIPQNSE
                     DLQVMLEAYLLDKAVYEISYELNNRPDWVVIPIRGIKHILKSG"
BASE COUNT          533 a          766 c          714 g          580 t
ORIGIN      
        1 acgcagccgc caacgtaacc gaaaccgcct ccgcgcgcgg tcaccggaat tctctcgtgc
       61 gaagcgaatt tcaacagctc gactacctgc cttgtcgatc ttgcgaagac gaccacttcc
      121 ggcgtctcgg cggcgaacca cttgtcgccg ctgtgcgcgg caagcgtatc ggcatcgtcg
      181 gcgacgatgt cgttgccgag taactcacgc aactttgacg caagtttcat cggctccaac
      241 gtcgcgcagg ccagttgtag ggcaagcgtt gcgcttgccc gaaggtggat cggccgctcc
      301 gcggccgatg ttgaacaaat ggcagtctcc ggctgcgtcg acttaacatc gagcgacgga
      361 gcgctcgatg aacctaacat gcaacgcgat agtgccgtga gcgctcgtcc atgcgcatga
      421 ggagcgcttc ggctttcggg taaatcaccg gcttgccttc gtccgtgcca gcgaattcct
      481 tgaccgccgc catcgagtcg aagcgaagta gcgtgacgaa ctccacttcc tcgccgtctt
      541 cattgatgaa caactccgcg ccgcgataac cattgatctt gcgcgccgca atcccgggaa
      601 agatctcgtc gcgcagcatc gtctcatagg tctgcgcgtc ctctcgtttt gtccagccgt
      661 gccaaatccg cgcgatcata aactcctgct cttgctcatg ctcttgctcg atctgttccg
      721 ctttcgatta cgagcatgag caggaggatg agtatgatta aaagaatcac atgcccataa
      781 tctggtaacc gccgtcgaca tagatcacct gaccggtgat cgcggcagcg gcgtcgctgg
      841 cgagaaatac gccggccgta ccgagctcgg ccggatcgca actgcgcttc aacggcgcgc
      901 gttcctgata atgttttagc atttgcgtga atccgctgat gccgcgcgcg gcgagcgttt
      961 gcaccggacc ggccgagatg cagttcacac ggattctttt tggcccgaga tcgtaggcga
     1021 gataacgcgt gctcgcttca aggctcgctt tggcgacgcc catcacattg taatgcggca
     1081 cgactttctc agcgccgtag taagtcatcg agacaatgct gccaccatta ctcattagcg
     1141 gtgcaacttc gcgcgccagc gcgacgagcg agtaggcgct gatatcgtgg gcgacgcgaa
     1201 aggcctcgcg cgtcgtgctg acaaaatcgc cttccagcgc ttccttcggt gcgaatgcga
     1261 tggaatgtag caaaagatcg acaaccgacg tctgtcccct cacctgctcg aaaaactttt
     1321 tgatctcctc gtcactcgac acgtcacatg ggaaaaacgg cgtctgctcg ccgaactcgc
     1381 caacaagttc ctcgacgttt tccttcagcc gctcgccctg ataattgaaa atcaacttcg
     1441 cgccggctgc cgcccaggcc ttggcgattg cccaggcgat actacgcttg ttcgcgacgc
     1501 cgaatacgac gccggttttc ccggccaaaa cttgtggagt catctcgttc agcacgcgat
     1561 cgcgtggcga cggcacacgc cggaaagcat ctgccacaac gcatcgagca cgcgcgcggc
     1621 ggccgcgcgc agctcctcga tgttccgact gtcgatcttg cgcgacagca tctcgcgctc
     1681 gacggccgaa tgctcgcgat ccgcttcgat gtggacgctg aagtactcgt aatgctcggg
     1741 attcgtgaag ccgtaatgct tcttcaaccc gtcgatcttg gattcacaga tcgccggaat
     1801 ttggctctca taggcgtaga gcgctgtgag accttcggca gtcgagccat tgccgcacac
     1861 cacgcggaac gtgtcgatca gatgctgcgt ctcgggttgt ttcggggtgt ttgcaagatc
     1921 gacattatca ataccgagcg catttgtgaa cctcttccaa agctctgggt ggttcggcga
     1981 gccggcttct tcatcaatta agtttttcaa caactgtttg cgcgtcgact ggtggtcaca
     2041 atgcgagtgg acagccgaga gatacgtcgg gaacgccgcg atatggtgat agtactgtcg
     2101 cgcgtaatcg gtcagcgctt ccctgctcaa ctcgcctcgc gcccaagcga gatagaacgg
     2161 atgcttcaac aaatgtttct ccgcgatgtc attgtcgatc tggtcgagat agctcatacg
     2221 gtgctcctaa tgctctatgg aaagccacag attggcacag attttcacag attaaaccaa
     2281 cacaattccg tatctgtgtt catcggtgtt cagctgtgac cgaagatttc tagcccgatt
     2341 tcaagatgtg tttgatgccg cggatgggga taacgaccca gtcggggcgg ttgtttagct
     2401 cgtagctgat ttcgtagacg gctttgtcga ggagataggc ttcgagcatc acctgcaggt
     2461 cctcggaatt ttgtgggatg aacacggcgc cggcagtggt gcgcagataa ctttgcagaa
     2521 acacgctgct aacgtggcgg taccagagat ccgcccaacg ctcgagaaac ggcacgtctt
     2581 cgctgcgcat ggc
//
LOCUS       DASTDG010000010        11173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_802962, whole genome shotgun sequence.
ACCESSION   DASTDG010000010 DASTDG010000000
VERSION     DASTDG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802962"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFO30_00295"
     CDS             complement(<1..647)
                     /locus_tag="VFO30_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9417753.1"
                     /translation="MKKKILVIDIGGSHVKLMISRQGKRRKFESGPHLKPQQFITESK
                     KLLADWKYDAIAMGFPAPVRKGKIAGEPKNLGQGWVGFDFRAAFRKPVRIINDAAMQA
                     LGSYHGGRMLFLGFGTGLGSALVWNRNVLSLELGDLPYIDNQSIEDQLSDAGLERLGE
                     KRWQTEVQRVVPCLKQALIADYVVLGGGNAKLLQKLPSGVALGHNRNAFTGGWRL"
     gene            complement(644..2866)
                     /gene="ppk1"
                     /locus_tag="VFO30_00300"
     CDS             complement(644..2866)
                     /gene="ppk1"
                     /locus_tag="VFO30_00300"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET9417754.1"
                     /translation="MAQQQPIPEVDEESPILPAHAGTQSTPERFGDPKNFLNRELSWL
                     EFNRRVLEEAQDPSQPLIERVKFLCIFSSNLDEFFEIRVAGIKQQIDSETSDVGPDGL
                     SPTETFNQIQRLTHELVTTQYELWNTELLPELAKNGIRVRNLSELNAKRAAWARKYFQ
                     DEVFPMLTPLAVDASHPFPDLLNKSHNLLVRAKTQRGGEPLLAIVQVPRVVPRLILMP
                     RGKSEDEPWNYIYLASLIKQHIAELFPGLILDAVHAFRVTRNSDLYIDEEEAENLLRT
                     IEQELRRSSRGNAVRLEVEADCPREFIALLLELFHLSEADVYKLNGPLSMTHLVPLIT
                     NDAFAKLKDRPFQPGRDPALPAHADMFEVMRRQDVLLHHPYDSFDPIVDLIDEAARDP
                     QVLAIKITLYRTSGDSPIVEALIEAANAGKQVTAIVELRARFDEAVNIEWSRRLEQAG
                     AHVIYGVVGLKTHCKALLIVRRDTDQLRRYVHLGTGNYHPRTARIYTDFSLLTTNPEL
                     TEEVATVFNTLTGLAGYPGLKKLMVAPFDLKKRLIAWIERERDHARAGRPARIIAKLN
                     SLVDQETIEKLYEASSAEVTIDLIVRGICCLRPKVPGLSEYIRVISIVGRFLEHSRIY
                     YFENGGQPELYLSSADWMPRNFVRRVEVAFPIETPALRDHIIKEVLPRFLHDRVKARE
                     LQPDGRYRRLKPEGPEPRAQAQWHFREVTRERAKKLGRKKTPPPDKLVPITIAPPKSS
                     "
     gene            complement(2914..3600)
                     /gene="phoU"
                     /locus_tag="VFO30_00305"
     CDS             complement(2914..3600)
                     /gene="phoU"
                     /locus_tag="VFO30_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9417755.1"
                     /translation="MTPPKHILGSFDEALASLRNNVLMMAGLTQRSLERAIKGLLQRD
                     DNLCANAIADDEEIDQLEKQIDKDGVDVLLRFQPVASDLRRVISAMKLSPNIERVGDQ
                     ATNIARRARKLNKHPPLPELELMVPIQAHAMAMFKDSIDAFVREDVDLARAVVARDDA
                     LDKMNKMANRSLTERMAQNPEQLRGYLNLIFVSRFLERVGDHATNIAEDAVYAAAAED
                     IRHQMLAASS"
     gene            complement(3695..4525)
                     /gene="pstB"
                     /locus_tag="VFO30_00310"
     CDS             complement(3695..4525)
                     /gene="pstB"
                     /locus_tag="VFO30_00310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9417756.1"
                     /translation="MISSASIRQQELASEGESTDTASERRPAFEVEQLSIFYGQKKAI
                     EDITLAVPSKMITAIIGPSGCGKSTLLRSLNRMHELIPNTRIEGKVLFAGKNIYSPEV
                     DPVVIRRRIGMVFQKSNPFPTMSIADNVLVGLKLNGVRDRTFLRERLEQSLRMSALWD
                     EVKDDLHKPGVSLSGGQQQRLCIARAIAVEPEVLLMDEPASALDPIATAKIEELIHEL
                     SANYTVVIVTHNMQQAGRCSDYTAFLYLGRLIEFGSTTKIFSNPSERRTEDYITGRFG
                     "
     gene            complement(4555..5415)
                     /gene="pstA"
                     /locus_tag="VFO30_00315"
     CDS             complement(4555..5415)
                     /gene="pstA"
                     /locus_tag="VFO30_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9417757.1"
                     /translation="MKAKRGDQHVWRKTKSALASGGAFVCALLVIAPLALVFFHLLRS
                     GASAVNWSFFTELPKPVGAPGGGMVNAIAGSFELLGLAALVGVPVGVLGGVYLAEYGT
                     VRGNWMLRFVADVLNGVPSIIWGVVVYGLIVLRFKSFSAYAGGLALGFIMIPLVMRTT
                     EEVLLLVPSGYREASLALGISRWKTIVHIVMKTAMKGIVTGVLLALARVAGETAPLLF
                     TAFGNRFWNHDLNEPIAALPLQIFSYAISPYDDWHRQAWAGALVLVVMIFFVNVLVRF
                     LTRERVVRVP"
     gene            complement(5412..6404)
                     /gene="pstC"
                     /locus_tag="VFO30_00320"
     CDS             complement(5412..6404)
                     /gene="pstC"
                     /locus_tag="VFO30_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9417758.1"
                     /translation="MSGMPLAIAPETPVARSTIDRMAPPSGVGDKIFKWLTLAMALAV
                     VALVFLVGWQLARGSSLAIQKFGFHFFTTSTWDPVAEQFGALPFIYGTAVSSLVALLI
                     AVPLSIATAVYLTELAPIWIRQPLVSLIEMLAAIPSVILGLWGIFVMIPWLRTHAFPF
                     LKQTLGWTPLFSGPIYGVSMLAGGIIIAVMILPIITSVSREILRSVPDLQREAAYALG
                     ATRWEVTRIAVLSYAKRGLFGAVVLGLGRALGETMAVTMVIGNTPQIRASLFAPGYTL
                     ASVIANEFTEATTDIYLQALFEIGLALFGITIIANLLAQLLLKTMGPARSGAVQ"
     gene            complement(6409..7374)
                     /gene="pstS"
                     /locus_tag="VFO30_00325"
     CDS             complement(6409..7374)
                     /gene="pstS"
                     /locus_tag="VFO30_00325"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9417759.1"
                     /translation="MSTTVSAQMTVNGAGATFPYPIYSKWFDEYAKVDPSVRFNYQSI
                     GSGGGQKQILAQTVDFGASDGPMSDDNLAKAPGKILHIPTVAGADVVAYNLPGNPSLK
                     LDADAIAGIFLGQIKKWNDPKLAALNAGVNLPDQEIVVVHRSDGSGTTYIWTDYLSKV
                     SPEWKEKVGTNTSVNWPTGLGGKGNEGVAGQIKQTPGALGYVELIYATQNRMPYAEVK
                     NAADKFVKPSVDSITAAMGTAQIPDDFRFSITNAPGADSYPIAGATWLLVYEQQKDAA
                     KGKKLVEFLKWALTKGEDMAKQLDYAPLPAALRERVLKRVDEIKM"
     gene            complement(7552..8805)
                     /locus_tag="VFO30_00330"
     CDS             complement(7552..8805)
                     /locus_tag="VFO30_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9417760.1"
                     /translation="MAWLLFTAIVGSTIVLAYVVWRKWIAPWHEIETLVRQVGRGEQP
                     RTFLVAGGREAERVGAALESILTRQRTLDRQLADYRSGQKAVFAAMQDGLLVVDGERR
                     IALLNRTFTHWFGVPENSLGAPLIETVRDPAIERLVDLTFQKREAVQREITIAERHLQ
                     MNGVPLGNKNGELNGAVILFHDITELKRLDEMRRDFVANVSHELRTPLSILRGYIETL
                     LDSGQTSRDELRRILSVMERHSKRLGLLLDDLLRLAKLESADPNLELSSVRLDELCLN
                     VARDWEKKLAAHNLRVLVDLAPGLPAVDADAPRLQEVLYNLLDNAVKYSPRGGEIRLR
                     VEPRGEEIALSVTDNGIGISKEDLPRIFERFYRAEKSRGGDNGGTGLGLAIVKHIAQL
                     HGGRVEAESEPGKGTTIRVILPAPL"
     gene            complement(8810..9496)
                     /locus_tag="VFO30_00335"
     CDS             complement(8810..9496)
                     /locus_tag="VFO30_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9417761.1"
                     /translation="MSSKTILIVEDEKDVVDLLSLNLRKAGGFVVATATDGATGLTKA
                     RDENPALIILDLMLPKMPGLEVCKILKTDPATRHIPIMMLTAKAEEIDRIVGLEFGAD
                     DYVVKPFSPREVVLRIRAILRRGAAEQSADRLSAGPITIDPARHRVSVEGKQVELTSL
                     EFRLLHMLMQRRGRVQERDRLLNDVWGYESVIDTRTVDTHVRRLREKLGKAGDVIETV
                     RGFGYRLREE"
     gene            complement(9527..9793)
                     /locus_tag="VFO30_00340"
     CDS             complement(9527..9793)
                     /locus_tag="VFO30_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417762.1"
                     /translation="MTDPGQAVTQEELAQLQRKFSEIKHSINNALAVMMALSEMSQRR
                     PDYAEKLATTVLSKAPQIVSSLQEFTQILNEKAGVKPAVADEAK"
     gene            complement(9955..11034)
                     /locus_tag="VFO30_00345"
     CDS             complement(9955..11034)
                     /locus_tag="VFO30_00345"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9417763.1"
                     /translation="MNSTIRSFAFLSFIIAAGEVFAADYSAPSEGEYTIRDFKFQSGE
                     TLPELRIHYRTLGKPEKDSQGKTTNAVLIMHGTTGNGAQFIRPEFAGELFGKDQPLDA
                     TKFFIVLPDGIGHGKSSKPSDGMHAKFPRYGYLDMIEAQYRLLTEGLGVNHARLVMGT
                     SMGGMHTWLWGELHPEFMDALLPLASLPAQISGRNRAWRRLIIDAIRNDPAWNGGEYK
                     TQPPSLRTAAEMLWLMSSNPVLRQKEAATLAEADVKIDKFVADYMKSGDANDVLYALE
                     ASRDYDPGPELEKIHAPLLAVNFADDLINPRELGILEREIKRVPHGRAIVVPFSDKTR
                     GHGSHTIAALWKDELLKLLNQPRAK"
BASE COUNT         2266 a         3238 c         3212 g         2457 t
ORIGIN      
        1 cacaagcgcc agccgccggt gaaggcgttg cgattatggc cgagcgcaac gccgctgggc
       61 aatttctgca aaagctttgc gttcccgccg ccgagcacga cgtaatccgc gatgagcgcc
      121 tgcttcaaac atggaacgac gcgttgcact tcggtctgcc atcgtttctc ccccaagcgc
      181 tccaagcccg cgtcgctcaa ctgatcttca atgctctggt tgtcgatgta gggcagatcg
      241 ccaagctcca atgagagaac gtttcgattc caaacaagtg ccgagcccag gccagtgccg
      301 aaaccgagga aaagcattcg tcctccgtgg taacttccca gcgcctgcat cgccgcatcg
      361 ttgatgatgc gcaccggttt gcggaaggca gcacgaaaat cgaagccaac ccatccttgg
      421 ccgaggttct taggctcgcc tgcgatcttg cctttgcgaa ccggcgctgg aaaacccatc
      481 gcgatcgcgt cgtatttcca atccgccagc agtttcttgc tttcagtaat gaattgttgc
      541 ggcttcaggt gtggccccga ctcgaacttt cgccgcttgc cttgccgcga gatcatcagc
      601 tttacgtgtg agccgccgat gtcgatgacg agaatttttt tcttcacgac gatttcggcg
      661 gcgcaatcgt gatcggaacg agtttatcgg gtggcggtgt tttctttcgg ccaagtttct
      721 tcgcgcgttc ccgcgtgacc tcgcggaaat gccattgcgc ctgggcgcgc ggttccgggc
      781 cttccggctt caaccgtcgg taacgtccat ccggctgtaa ctcgcgcgct ttgacgcgat
      841 cgtgcaaaaa tcgcggaagc acttccttga tgatgtggtc gcgcaacgcg ggcgtctcga
      901 ttgggaaagc cacttcaacg cgcctgacaa aatttcgcgg catccagtcg gcactcgaca
      961 aatacaattc tggttggccg ccattttcaa aataataaat gcggctgtgc tcgagaaagc
     1021 ggccgacaat gctgatcaca cgaatatatt cgctcaggcc cggcactttc ggtcgcagac
     1081 agcaaatgcc gcgaacgatc agatcgattg tcacttctgc ggacgacgct tcgtacagtt
     1141 tttcaatggt ctcttgatcg acaagcgaat tgagcttcgc gatgatgcgg gccggccggc
     1201 cggcccgggc gtgatcgcgc tcgcgctcga tccaagcgat cagccgcttc ttcaaatcga
     1261 acggtgcaac catcaatttt ttcaggccgg ggtaacccgc tagtccagtg agcgtgttga
     1321 agacggtggc gacttcttcg gttaactctg ggttcgttgt cagcaaactg aagtcggtat
     1381 aaatgcgcgc ggtgcgggga tgataattgc ccgtgccgag atggacgtag cggcgcagct
     1441 ggtcggtatc gcgccgcaca atgagcagcg ctttgcaatg tgtcttcaac ccgaccacgc
     1501 cgtaaatgac atgcgcgcca gcttgctcga gccggcgcga ccattcgatg ttcaccgctt
     1561 cgtcgaagcg agcgcgcagc tcgacgatgg ccgtgacctg cttgccggca ttcgccgctt
     1621 cgatcagggc ctcaacaatc ggcgaatcgc cgctggttcg atagagcgta attttgatgg
     1681 cgagcacttg cgggtcgcgc gctgcttcgt cgataagatc gacaatcgga tcgaagctgt
     1741 cgtagggatg atgcaggagc acatcctgac gccgcattac ctcgaacatg tcggcgtgcg
     1801 ctggcaatgc cggatcgcgc cccggttgaa acggtcggtc cttcagcttg gcaaaggcgt
     1861 cattcgtgat cagcggcacg agatgagtca tcgagagcgg accattcagc ttgtagacgt
     1921 cggcttcgct caaatggaag agctccagga gcagggcgat gaattctctc ggacaatcag
     1981 cttccacttc caagcgaacg gcgttgccgc gactggaacg tcgcaattcc tgctcgatcg
     2041 tgcgcagaag attttctgcc tcctcctcat cgatgtagag atcgctgttg cgcgtgacgc
     2101 gaaaggcgtg caccgcatca aggatcaagc ccggaaatag ttcggcgatg tgctgtttga
     2161 tgagcgaggc gagataaatg tagttccacg gttcgtcttc acttttgccg cgcggcatca
     2221 ggattagccg cggaacgacg cgcggaactt gcacaatcgc cagtaacggc tcaccgccgc
     2281 gctgagtttt tgcccgcacc agcaggttat gacttttatt cagaaggtcg gggaacggat
     2341 gactggcgtc caccgccagc ggagtgagca tcgggaaaac ttcgtcctga aagtatttgc
     2401 gcgcccaagc ggcgcgtttg gcgttcagtt ccgagaggtt gcgaacacga atgccgtttt
     2461 ttgcgagttc cggtagcagc tctgtgttcc aaagctcgta ttgcgttgtg accagctcat
     2521 gggttagccg ctgaatctgg ttaaaggttt cagtgggact gaggccatcc ggcccaacat
     2581 cgctcgtttc gctgtcgatc tgttgtttga tcccggcgac ccggatttca aaaaactcat
     2641 ccaaattcga gctgaagatg caaagaaact taacccgctc gatcagcggc tggcttggat
     2701 cctgcgcttc ctcgaggaca cggcgattga attcgagcca gctcagctcg cgattaagga
     2761 aatttttcgg atcgccaaaa cgttcgggcg tcgactgcgt gccagcgtgc gcgggcaaga
     2821 taggggactc ttcgtcgact tcgggaatgg gctgctgttg cgccattgcc gcaagatcga
     2881 caatcaatcc cgcataagca atgcgtgaac tcttcagctc gaggcagcga gcatctggtg
     2941 gcgaatgtcc tccgccgccg ccgcgtaaac agcgtcctcc gcgatgttcg tggcatgatc
     3001 gccgacccgc tccaaaaaac gtgagacaaa gatcaggttc aaatagccgc gcagttgttc
     3061 cgggttttgg gccattcgtt cggtcagact gcgattggcc attttgttca tcttgtccag
     3121 cgcgtcgtcg cgcgccacaa ccgcgcgcgc aagatcgaca tcttcgcgaa caaaagcgtc
     3181 gatgctgtcc ttaaacatcg ccatggcgtg cgcctgaatg gggaccatca attccagttc
     3241 cggcagcggt ggatgtttat tcaatttccg ggcacgccgc gcaatgttcg ttgcctgatc
     3301 gcccactcgc tcgatgttcg gcgaaagctt catcgccgaa atgacgcggc gcagatcgga
     3361 cgccaccggt tgaaaacgca agagcacgtc cacgccgtct ttgtcgattt gtttctcgag
     3421 ctgatcgatc tcctcgtcat cagcgatggc gttggcgcaa aggttatcat cgcgctggag
     3481 caggcctttg atcgcgcgtt ccaggctgcg ctgcgtcaat cccgccatca tcaacacgtt
     3541 gttgcgcaat gaagcgagcg cttcgtcgaa gctgccgagg atatgttttg gtggagtcat
     3601 ctcgcgcggc ggcagaattg ccgggcaggt gcatcgcctg ctgattcagt cctggcaagc
     3661 ggagattttg ccccacaaca tcgtccgtcc ctgatcatcc gaatctcccg gtaatgtaat
     3721 cttccgttcg acgctcgctc gggttggaga aaattttcgt cgttgaaccg aattcgatca
     3781 gccggccgag atacaggaag gcggtgtaat cggagcagcg gccggcctgt tgcatattgt
     3841 gcgtcacgat cacgaccgtg taattcgcac tcagctcgtg gatcagctct tcgattttcg
     3901 cggtcgcgat tgggtcgagc gcggacgccg gttcgtccat taataacact tccggttcga
     3961 ccgcgatggc gcgcgcgatg cagagccgtt gctgttgccc gccagaaaga ctgacgcccg
     4021 gtttgtgcag atcgtctttt acttcgtccc aaagcgccga catccgcagc gattgctcga
     4081 gacgctcacg caaaaacgtg cggtcgcgca cgccgttcaa tttcaaacca acgagcacgt
     4141 tgtcggcgat cgacatcgtc gggaatggat tcgatttttg aaaaaccatg ccgatgcgcc
     4201 ggcggatgac aaccgggtcg acctcaggcg agtaaatatt tttgccggca aacagcacct
     4261 tgccttctat ccgggtgttg ggaatcagtt cgtgcatgcg attgagcgag cgcaagaggg
     4321 tcgacttgcc gcaaccgctc ggaccgatga tagcggtaat cattttcgac ggcacggcga
     4381 gagtgatgtc ctcgatcgct tttttctggc cgtaaaagat cgacagttgt tcaacttcaa
     4441 acgcgggccg gcgctccgat gccgtgtcag tcgattcacc ctcggaagcc aattcttgct
     4501 gccggattga ggcggaggag atcatcggtc gttgaattat ggcggcgctt tccatcaggg
     4561 aactcggaca actcgttcgc gggtgagaaa gcgaaccaga acattaacga agaaaatcat
     4621 taccaccagc accagtgcgc cggcccaagc ctgccggtgc cagtcgtcgt agggtgaaat
     4681 ggcgtaactg aaaatctgca aagggagggc cgcgatcggt tcgttcagat catggttcca
     4741 aaagcgattt ccgaaagcgg tgaaaagcaa cggtgcggtt tcgccggcga cacgtgcgag
     4801 cgccaggagc acgccggtga caattccctt catcgccgtt ttcatcacaa tatggacgat
     4861 ggttttccag cgactgatcc cgagcgcgag cgaagcttcg cgataaccgc tcgggacaag
     4921 caaaagcact tcttccgtcg tgcgcatgac cagcggaatc ataatgaatc cgagcgcgag
     4981 cccgccggcg taggcggaaa aactcttaaa gcgcaagacg attaaaccgt aaaccaccac
     5041 gccccagatg atggagggga cgccgttgag cacgtcggca acgaaacgta gcatccaatt
     5101 cccacggaca gttccatatt cggcgagata aacgccgccc aacactccaa ccggcacgcc
     5161 gaccagcgcg gccaggccaa gcaattcgaa cgagcccgca atcgcgttga ccatccctcc
     5221 accaggcgcc ccgactggtt ttggtagttc ggtaaagaac gaccagttca ccgcgcttgc
     5281 gccgctgcgg agcaaatgaa aaaacacgag cgcaagcggc gcgatcacga gcaatgcgca
     5341 cacgaacgcg ccgcccgacg caagtgcgct cttcgttttc cgccaaacgt gctggtcgcc
     5401 gcgcttcgcc ttcattgcac agcgccactt ctggccggtc ccattgtttt cagcagcagt
     5461 tgcgcgagaa gattcgcaat gatggtgatc ccgaaaaggg cgaggccgat ttcgaacaac
     5521 gcttgcaggt aaatgtcggt cgttgcttcg gtgaactcat tcgcgatcac gctcgcgagc
     5581 gtgtagcccg gcgcaaaaag cgaagcgcga atttgcggtg tgttcccgat caccatcgtg
     5641 accgccatgg tttcgcccag ggcgcgaccg aggccgagaa ccaccgcgcc gaacagtcct
     5701 cgctttgcgt aactgagcac ggcgatgcgc gtcacttccc accgcgttgc gccgagcgca
     5761 taggccgctt cgcgctgcaa atccggcacg ctgcgcaaaa tctcgcgcga gaccgaagtg
     5821 atgatcggca agatcatcac cgcgatgatg atgcctcccg ccagcatgct gacgccgtaa
     5881 attgggccac tgaataatgg cgtccagccg agcgtttgtt tcagaaatgg aaacgcgtgt
     5941 gtgcgcagcc acggaatcat cacgaaaatc ccccagaggc cgagaatcac acttggaatg
     6001 gcggccagca tttcgatgag ggagacaagc ggttgccgga tccaaatcgg ggcgagctcg
     6061 gtcaaataaa ctgccgtcgc gatgctcagc ggtacggcga tgagcagggc gacgagcgag
     6121 gaaacagccg tgccataaat gaagggcagc gcgccaaatt gctccgcaac cgggtcccat
     6181 gtcgatgttg tgaaaaaatg aaatccgaat ttttggatgg cgagggatga gccgcgcgcc
     6241 agttgccagc cgaccaggaa caccagtgcc acgacggcca gcgccatggc gagtgtaagc
     6301 cacttaaaga ttttatcgcc cacgccggac ggcggcgcca tccgatcgat tgtcgatctt
     6361 gcaacgggcg tttccggcgc gatggcgagg ggcataccgg acatccgatt acattttgat
     6421 ctcgtccacc cgtttcagga cgcgctcgcg caatgccgcc ggcaacggcg cgtaatccaa
     6481 ttgtttcgcc atatcttcgc ctttggtcag tgcccatttt agaaattcga cgagcttttt
     6541 gcctttggct gcgtcctttt gctgttcgta aactagaagc caggtcgcgc ccgcaatcgg
     6601 atacgaatcg gcgcccggcg cgttggtgat ggaaaaccgg aaatcgtccg ggatctgcgc
     6661 cgttcccatc gccgccgtaa tcgaatcgac cgacggcttg acaaatttgt cggccgcgtt
     6721 ttttacctcc gcgtacggca tgcggttctg ggtcgcgtag atcaactcga cgtagccgag
     6781 cgcgccgggc gtctgcttga tctgtcctgc gacaccctcg ttacctttgc caccgagtcc
     6841 ggtcggccaa ttcactgacg tgttcgtgcc gactttctct ttccattccg gactcacttt
     6901 gctcaggtaa tcggtccaaa tgtaagtcgt gccgctgcca tcggagcggt gaaccacgac
     6961 gatttcctga tcgggtaaat tcacgccggc attcagcgcc gcgagtttcg gatcgttcca
     7021 cttcttgatt tggccgagga aaattccggc gatcgcatcg gcatcgagct tcaaagaggg
     7081 attgcccggc agattgtagg cgacgacgtc ggcgccggcc acggttggaa tgtgcaagat
     7141 tttaccgggc gccttcgcga ggttatcgtc gctcatcggt ccgtccgatg cgccgaagtc
     7201 cactgtttgc gcgagaatct gtttttgccc gccgcccgag ccgatcgatt gataattgaa
     7261 gcgcactgat ggatcgactt tcgcgtattc gtcgaaccat tttgagtaga tcggatatgg
     7321 aaacgttgca cccgcgccgt tgaccgtcat ctgcgcggag acggtggtgc tcaacccgca
     7381 cgcgattgtt aatccgagaa gaagtgttct gccgaaccgg tggagtttgg tcatgcgaca
     7441 agattgccgc acgcgtgttc tgccggtcaa aaaatttact gttacgtttg tgtgacagcg
     7501 tgtagccacg tgcctgtggc acgtccgcgc tgacgtcgca tcgcgacgtg gctacagagg
     7561 cgcgggcaag ataacgcgaa tggtggtgcc tttgccgggt tcactttcag cttcgacgcg
     7621 accgccatgc agttgcgcga tatgtttgac gatcgcgagg ccgaggccgg tgccgccgtt
     7681 gtcgccgccc cgcgatttct cggcgcgata aaaacgctcg aagatccgcg gcaaatcctc
     7741 tttgctgatc ccgataccgt tatccgtgac gctcaacgcg atttcctcgc cgcgcggttc
     7801 aacgcgcagc cgaatctcgc cgcctcgcgg cgaatatttc accgcgttat cgagcaaatt
     7861 gtagagcact tcctgcagtc gcggcgcgtc ggcgtcgacc gctggcagac cgggtgcaag
     7921 atcgacaagc acgcgcaggt tgtgcgcggc gagtttcttc tcccagtcgc gcgcgacgtt
     7981 gaggcaaagc tcgtccagcc gcacactcga caactcgagg ttcgggtctg ccgattccag
     8041 cttggccagg cggagcagat cgtcgaggag caggccgagc cgctttgaat gccgttccat
     8101 cacgctgaga atgcgccgca attcatcgcg cgacgtttgc cccgaatcga gcaacgtctc
     8161 gatgtaaccg cgcaagatcg acaacggtgt gcgcaattca tgcgagacgt tcgcgacaaa
     8221 atcgcgacgc atctcatcta gccgttttaa ctcggtgata tcgtgaaata aaatgactgc
     8281 gccgtttaat tcgccgtttt tgttcccgag cggcacgccg ttcatctgga gatgtcgttc
     8341 tgcaatcgtg atctcgcgct gaacggcttc acgtttctgg aaggtgagat caacgagccg
     8401 ttcgatggct ggatcgcgca ccgtctcgat tagcggcgca cctaacgagt tctcgggaac
     8461 gccgaaccaa tgcgtgaagg tgcggttgag gagggcgatg cggcgttcgc cgtcgaccac
     8521 caacagaccg tcctgcatcg cggcgaaaac cgctttctgg ccggacctgt agtcggcaag
     8581 ttgtcgatct aatgttcgct gccgcgtcag gatactttcc agcgcggcgc cgactcgttc
     8641 cgcttctctg ccgccggcga cgagaaatgt gcgcggctgc tcgccgcgtc cgacttgccg
     8701 caccagcgtc tcgatctcgt gccagggcgc gatccacttt cgccagacga cgtatgccaa
     8761 aacgattgtt gatccaacaa tcgcggtgaa tagcagccag gccatcgcct tactcttcgc
     8821 gtaagcgata gccgaacccg cgcaccgtct cgatgacatc gccggctttg ccgagtttct
     8881 cccgcagccg gcgcacgtgc gtgtcgaccg tgcgagtatc gatcacgctt tcatagcccc
     8941 agacgtcgtt gagcaaacgg tcgcgttcct gcacgcgtcc gcgccgctgc atgagcatgt
     9001 gcaagagcct gaactcgagg ctggtgagct cgacctgctt gccttccacc gagacgcgat
     9061 gccgtgccgg atcgatcgtg atcggcccgg cactaagccg gtcggcgctt tgctcggccg
     9121 cgccgcgccg cagaattgcc cggatgcgca aaacgacttc gcgcggactg aacggtttca
     9181 cgacgtaatc gtcggcgccg aattccaggc ccacgatccg atcgatttct tcggctttgg
     9241 cggtcaacat catgatcgga atgtggcgcg tggcagggtc agtcttcaga attttgcaga
     9301 cctccagtcc gggcattttc ggcaacatca gatcgagaat aataagcgcc ggattctcgt
     9361 ctcgcgcttt cgttaggccg gttgcgccgt ccgtggcggt cgcaacgacg aacccgccgg
     9421 ccttgcgcag attcagacta agaagatcga caacgtcctt ctcgtcttcg acaatgagaa
     9481 tggttttgct cgacactgcg accatgcaga gcagggcggg cctttactac ttcgcttcgt
     9541 ccgctaccgc cggtttaacg ccggccttct cgttcaagat ttgcgtgaat tcctgtaggc
     9601 tggacacgat ctggggcgcc ttgctcaaaa ctgtggtcgc cagtttttcg gcgtaatccg
     9661 gccggcgctg tgacatttcg gaaagtgcca tcatgacggc gagcgcgttg ttgatggaat
     9721 gtttgatctc gctgaatttg cgctgcaatt gcgcaagctc ttcctgggtg acggcttggc
     9781 cggggtcagt cataaaaggg gcgattcgat tgggagaggc acgggactgt tgtgcagtgc
     9841 aaacaactgg tcagcaggct ggtgtgtgcg gaaactagca atattgctgc tttccgccag
     9901 cacaatccga accaagtgcc gagcttcggg acgagtcatc gatggttaac attcctactt
     9961 cgcccgcggc tgattcagca acttcagcag ttcatccttc cacagcgcag cgatggtgtg
    10021 actgccgtgc ccgcgtgttt tgtcgctgaa gggaaccacg atcgccctgc cgtgcggcac
    10081 gcgtttgatc tcccgctcga gaatgccgag ctcgcgcgga ttgatcaggt cgtcggcaaa
    10141 attaaccgcc agcagtggcg cgtgaatttt ctcgagctcc ggcccgggat cgtaatcgcg
    10201 cgacgcttcc agcgcgtaga gcacgtcatt cgcgtcgccg cttttcatgt aatcggcgac
    10261 gaacttgtcg atcttcacgt cggcttcggc gagggtcgcc gcttcttttt gccgcaaaac
    10321 cggattgctg ctcattaacc agagcatctc ggcggcggtg cgcagactcg gcggttgggt
    10381 cttgtactcg cccccattcc atgccggatc gtttcggatc gcatcgatga tgagccgccg
    10441 ccaggcgcgg ttacgcccgg agatctgcgc gggcaggctc gccagcggca agagcgcatc
    10501 catgaactcc ggatgcaatt cgccccagag ccaggtgtgc attccgccca tcgacgtgcc
    10561 catgacgagg cgcgcgtgat tcacgcctag cccttcagtg agcagacggt actgcgcttc
    10621 gatcatgtcg agataaccgt agcgcggaaa tttcgcgtgc attccgtcgc ttggcttgct
    10681 cgatttgccg tgaccgatcc cgtccggcag cacgatgaag aatttcgtcg catcgagcgg
    10741 ctgatctttc ccaaacagct cgccggcaaa ctcggggcga atgaattgcg caccgttgcc
    10801 agttgttcca tgcattatca gaactgcgtt cgttgttttg ccctgcgaat ctttctccgg
    10861 ttttccgagc gtccgataat gaatgcgtaa ttccggcaaa gtttcgcccg actgaaattt
    10921 gaaatcgcga atggtgtact cgccttcgct gggcgcggaa taatctgcgg caaatacttc
    10981 gcccgcggcg atgatgaatg aaagaaatgc gaaagatcgg atggttgagt tcatattagt
    11041 cgcggggttg gcgttcggct tgaattcttt tgagcagctc ggtgtgggtt cgttccaggt
    11101 cttgagcgaa tttcacagtt gcttgtagag cttcgggata catctcgaac cgttttcgca
    11161 atttcttggt ggc
//
LOCUS       DASTDG010000011         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1406296, whole genome shotgun sequence.
ACCESSION   DASTDG010000011 DASTDG010000000
VERSION     DASTDG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406296"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFO30_00350"
     CDS             complement(<1..221)
                     /locus_tag="VFO30_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HET9417764.1"
                     /translation="MPQNGRTIRDKILSLLRQDDYRPLDKNEIARKLALKGDERVTLR
                     HTLRELERAGGIARIRKNRYVLPAEADLVT"
     gene            286..807
                     /locus_tag="VFO30_00355"
     CDS             286..807
                     /locus_tag="VFO30_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9417765.1"
                     /translation="MKAVAIGGCALAVVAVAALAETGGGTMKITSSAFNEGGMIPDKY
                     AKDGQNVNPPLRIEDTPADAKSLLLIMDDPDAPVGLFTHWLLWNIDPKTTEIAENSVP
                     SGAVQGTNDYPNLGYGGPQPPSGTHRYYFKIFALDRTLDLKSGAKRKELDAAMRGHVI
                     AQGELMGRFSHKK"
     gene            921..1763
                     /gene="folP"
                     /locus_tag="VFO30_00360"
     CDS             921..1763
                     /gene="folP"
                     /locus_tag="VFO30_00360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9417766.1"
                     /translation="MRVRQWKIGEAVVDLAQHGMIIGVLNVTPDSFSDGGRFFDLDAA
                     IVHGTEMAKQGADIIDVGGESTRPGAEPVSPHEELRRVVPVIERLRRKISKLISIDTS
                     KAEIARAALEAGACIINDVTGGRADPEMLPLVAKARAAFVLTHMQGTPRTMQMEPRYN
                     DVVAEVGDFFLQQYGRALECGVDPMGVAFDPGIGFGKTPAHNLELLRNLERLRVHDRP
                     IVVGVSRKSFLGKLSGATDVGDRLAPTVALTALLRAHGADVFRVHDVKENVAALRIAE
                     ALLS"
     gene            1760..2635
                     /gene="cdaA"
                     /locus_tag="VFO30_00365"
     CDS             1760..2635
                     /gene="cdaA"
                     /locus_tag="VFO30_00365"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET9417767.1"
                     /translation="MIQQLLHLWLRSWRSIFEIILLSVVIYYGYLYFRGTRGAKVLTG
                     LAIVFLTLTLISQLLNLVVIGWIVRSFSVFLAVALVVIFQPELRRALAELGGHPIFSL
                     TSEKRETVHDLAEAVTQLANKQFGALIALERDTSIRVYEETGVRLDGEFSVELILTIF
                     HPKAALHDGGVIIRNGRIAAAACIFPVSQRETLDRSLGLRHRAGLGITEESDAVAVVV
                     SEETGGISICHRRRIERNFTPETFRQRIAEILLHANYEETDSEKLAGEIDLRPARDGR
                     LVPDQKERSDDALAV"
     gene            complement(2678..3130)
                     /locus_tag="VFO30_00370"
     CDS             complement(2678..3130)
                     /locus_tag="VFO30_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417768.1"
                     /translation="MAKKTKRKLAHPKLPMQRQLNLRHEGRYFDLRPIFDQLNERHFG
                     GRLRGYEVVWGRRRKHRPKEYFVFGTIQEEDRVIRINPALDQPFVPRWFLRYVMYHEM
                     LHSVVPDKTLAGGRRRVHTERFNRREREFPGYRRARRWEDENLARFLR"
     gene            3415..4527
                     /locus_tag="VFO30_00375"
     CDS             3415..4527
                     /locus_tag="VFO30_00375"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9417769.1"
                     /translation="MTPFLRKLLGLNWLLVLNMLALGVFGVIAIYSATYMREDSVAAD
                     FWRKQANWVLVGFFAFMLVSLIDYRWIRWGALPIYLAGLFFLILTKFIGTKVYGARSW
                     LHLGPVNFQPAQLAVIAGIMVLALFLSQFRQMYPMLRLLLCGAIAGAPCLLILLQPDL
                     GEVIIWVPVLLALLFAAGLPLRYLICIILIGIAFIPLAIHLGLKPYQQQRITAFINPD
                     IDKQGASWAINQSLIAIGSGGWSGKGFKAPNTQIELGFLPATAVHNDYIFSAIGEQWG
                     FVGGMFLLGGFALLLLTCLFVAFFAADQFGMLLVVGITALIFTHIFQNVGMTIALLPI
                     TGVPLPLVSYSGSFVLMIMFGLGIVNSVWVHRKAAD"
     gene            4589..5314
                     /locus_tag="VFO30_00380"
     CDS             4589..5314
                     /locus_tag="VFO30_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9417770.1"
                     /translation="METDYKVKLEIFEGPLDLLLYLIKRDEIDIYDISLERITRQYLE
                     YLQAFKELKIDVAGEFVAMAANLIYLKSRSLLPRDQQPPEEDAEEHDPRWDLIRQLIE
                     YKKFKEAAADLNVRALARERMFSRQGDPAATPEQPFRLDEVGIFQLIHAFQNAIKRVE
                     ARQSVQEIFGDQFTVSEKIDIILERVANGATLRFSDLFSAVASRVEVVVTFLALLELI
                     RLQQVHALQRNFFDEIEIAPAVA"
     gene            5331..>5904
                     /locus_tag="VFO30_00385"
     CDS             5331..>5904
                     /locus_tag="VFO30_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9417771.1"
                     /translation="MPRLLIAGCGYVGAATADLLFTRGWEVEGWTHSQESAGKLGRKA
                     YQVHAVDLTERADVANCSTNFDAVIHCASTHGGGAELYRRLYLDGARNLLERFAGSTI
                     LFTSSTSVYAQSDSSWVHEESATEPAPESGRVLLQTEKLVLAHGGIVARLAGIYGPGR
                     SVLLEKFLSAEAFIDPEHDRFINQVHRDDIA"
BASE COUNT         1175 a         1648 c         1747 g         1334 t
ORIGIN      
        1 gtcacgagat cggcttccgc gggtaggacg taccggttct tacggatgcg cgcgatcccg
       61 ccggcgcgtt ccagctcgcg caacgtgtgc cgcagcgtca cgcgctcatc gcctttcaat
      121 gccagcttgc gcgcgatctc gtttttatcg agtgggcgat aatcgtcctg tcgcagcaga
      181 gagagaatct tgtcgcgaat tgtgcgtccg ttttgcggca tccgagcgaa aatgtactgt
      241 cagaccagat tgtcgatcta acgaaatgca actggagatc gagacatgaa agcagtcgct
      301 atcggcggct gcgcgctggc ggtcgtcgcg gtcgccgcgt tggccgaaac aggaggtgga
      361 acgatgaaga tcacgagctc agcttttaat gaaggcggaa tgattcctga caaatacgcc
      421 aaggacggac agaacgtaaa tccgccacta cggatcgagg acacgccggc ggacgcaaaa
      481 agtctgttat tgatcatgga tgatccggac gcgccggtcg ggctcttcac gcattggctg
      541 ctctggaaca tcgatccgaa gactaccgag atcgcggaga acagcgtacc cagcggcgcg
      601 gtacagggga caaatgatta tccgaacctc ggttacggtg gtccgcagcc gccgtcgggg
      661 acgcatcgct attatttcaa aatctttgcg ctcgaccgca cgctcgatct gaaatccggc
      721 gctaagcgca aggagcttga tgcagcgatg cgcggtcatg tgatcgcgca aggcgaattg
      781 atgggccgct tttcgcacaa gaagtaatgc aattctgacc ctcaccaggc ggctttgccg
      841 cccgcctctc agggagagga ttgaggtgag ggcagcgacg gtttcgaata acatcacgcg
      901 tgtgatagtc tgaacgcgtg atgcgagtgc gacagtggaa gatcggcgaa gcggttgttg
      961 atcttgccca gcacgggatg atcatcggtg tgctcaacgt cacgcccgat tccttctcgg
     1021 acggcggtcg atttttcgat cttgatgccg cgattgtgca cggcaccgaa atggccaaac
     1081 aaggcgccga catcatcgat gtcggtggcg aatcgacccg tcctggcgcg gagccggttt
     1141 caccgcacga agaactgcgg cgcgttgttc cggtgattga aagattgcgt cgcaagatca
     1201 gcaaacttat atcgatcgat acgtcaaagg ctgaaattgc gcgtgcagcg ctcgaggccg
     1261 gcgcctgcat tatcaacgac gtgaccggcg gacgcgctga tccggaaatg ctgccgctgg
     1321 ttgctaaagc cagagcggca tttgtgctca cgcacatgca aggcacgcca cgcacgatgc
     1381 agatggagcc gcgctacaat gatgtggttg cggaagtggg tgattttttt ttacaacaat
     1441 atggccgcgc cctagagtgc ggcgttgatc ccatgggcgt ggcgtttgat ccgggcatcg
     1501 gttttgggaa aacgcccgcg cataacctcg aactgctgcg aaacctggag cggctccggg
     1561 tccatgaccg tccaatcgtc gtcggtgttt cgcgcaaatc atttctcggt aaactgagcg
     1621 gcgccacgga cgtgggcgat cgcctcgcgc caaccgtcgc tttgactgcg ctgttgcgtg
     1681 cgcatggtgc cgatgttttt cgcgtgcatg acgtcaaaga gaacgtagct gcattgcgca
     1741 tcgccgaggc actcctgtca tgatccagca actgctccac ctctggttaa gaagctggcg
     1801 gagcattttc gagatcatcc tgcttagcgt ggtgatatac tatggttatc tctactttcg
     1861 tggcacacgc ggcgccaaag tgctcaccgg actggcgatt gtttttttga cgctgacgtt
     1921 gatctcgcaa ctgctgaacc ttgtcgtgat cggctggatt gtgcgcagtt tctcggtgtt
     1981 cctcgccgtt gcgctggtgg tgatcttcca accggaattg cgccgcgcct tggccgagct
     2041 cggcggacat cccattttct cgctgacgag cgagaaacgc gagacggtgc acgatctcgc
     2101 cgaagcggtc acgcagctcg cgaataaaca gttcggtgcg ctgattgcac ttgagcgcga
     2161 cacgtcgatt cgcgtttacg aagaaaccgg tgtgcggctc gacggcgaat tttcggtgga
     2221 gctgatcctg acgatttttc atcctaaggc ggcactgcat gacggcgggg tcattatccg
     2281 taacggtcgt atcgccgccg ccgcttgcat tttcccggtc agtcaacgcg aaacgctcga
     2341 tcgcagcctc ggcttgcgtc accgcgccgg gcttggaatc accgaggaat cggacgcggt
     2401 tgcggtcgtg gtgtcggagg agaccggcgg tatttccatt tgtcatcgcc gccggattga
     2461 gcgcaatttc actccggaaa cattccgcca gcggatcgcg gagattttat tgcatgccaa
     2521 ctatgaagag actgattctg agaaactggc aggcgaaatt gatctccgtc ctgctcgcga
     2581 cggccgtttg gtacctgatc aaaaagaacg tagcgacgac gcccttgccg tctgaagcac
     2641 gctccagccc agtgagcgaa agtcgctgaa gcgagcttca gcgcaaaaaa cgcgcgaggt
     2701 tttcgtcttc ccagcggcgc gcccgccgat aaccgggaaa ctcgcgctcg cggcgattga
     2761 atcgctcggt gtgcacgcgc cgtcgtccgc cggctaaagt tttgtcggga acgaccgagt
     2821 ggagcatttc gtggtacatg acgtagcgca ggaaccagcg cggcacgaac ggctggtcga
     2881 gcgcgggatt aatccggatg acccggtctt cttcctggat ggtgccgaag acaaaatatt
     2941 ctttcggacg atgcttgcgc cggcgccccc agacgacttc gtagccgcgc aatcgcccgc
     3001 cgaagtggcg ctcattgagc tggtcgaaaa ttgggcggag gtcgaagtac ctgccttcgt
     3061 gccggaggtt gagctgccgc tgcattggca gcttgggatg agcgagtttg cgttttgttt
     3121 ttttggccat gagcgcttgc gaggagggat gcgacaaatc gggagaagca ttttagtggc
     3181 gccggccgga ctgacaagcg acgcgatcga gattttgcga tttttccgac aagaacctgg
     3241 agatcagagg cttatctgcc cagaaacccg gtaacttggc tttctcgcag aactcattcg
     3301 caagcaacat tgttggtcgg ggggcagtgc gctggcgcat ttacgaccac cggaacatgc
     3361 gtatattccc gcgctttctt tatggggtcc gaacctcagc cgaagtaaac cgacatgacc
     3421 ccgtttctgc ggaaattact cggccttaat tggcttctcg tgctcaacat gctggcgctg
     3481 ggcgtattcg gcgtcatcgc gatttacagt gccacttaca tgcgcgagga ctcggtcgcg
     3541 gcggacttct ggcgtaagca agccaattgg gtgctggtgg ggttcttcgc cttcatgctg
     3601 gttagtttga tcgactaccg ctggattcgc tggggcgcgt tgccgatata tctcgccggg
     3661 ctgttctttt tgatcctgac gaagttcata ggcacaaagg tttacggcgc gcgcagctgg
     3721 ttgcacttgg ggccggtcaa ttttcaacct gcgcagcttg ccgtcatcgc cggcataatg
     3781 gtcctggcat tattcctgag ccaattccgg caaatgtacc cgatgctgcg cttgctcttg
     3841 tgtggcgcga ttgccggcgc accatgtttg ttgattctgc tccaaccgga ccttggcgaa
     3901 gtcatcattt gggtgccggt gctgctcgca ttgctcttcg cagccggatt gccgctgcga
     3961 tatttgatct gcatcattct cattggcatt gcgtttatcc cgctcgcgat tcatctcggg
     4021 ctgaagcctt accaacagca gcgcatcact gccttcatca atcccgatat cgataaacag
     4081 ggcgcctcct gggcgatcaa tcagtccctc attgcgatcg gctccggcgg ctggtcaggc
     4141 aaaggattta aagcgccgaa cacgcaaatc gagcttgggt ttctgcccgc gaccgcggtg
     4201 cacaacgact acattttttc cgctatcggt gagcagtggg gattcgtcgg cgggatgttc
     4261 ctgctcgggg gatttgccct gctgcttttg acctgtcttt ttgtcgcgtt tttcgccgcc
     4321 gaccaattcg gtatgcttct cgttgtcggc atcactgcgc tcatcttcac ccacattttc
     4381 cagaatgtcg ggatgaccat cgcgctgctg cccatcacgg gcgtgcccct gccgctcgtc
     4441 agctacagcg ggtcgttcgt gctcatgatc atgttcggcc ttggtatcgt gaacagcgtc
     4501 tgggtgcatc gtaaggcggc cgactgatcc gggtcgtttt ttgcaagatc gacaatcgct
     4561 cgtccgtcga gttaagtcgc tgcgaggtat ggagaccgat tacaaggtca aactcgaaat
     4621 tttcgaaggc ccactcgacc tgctccttta cctaattaag cgcgacgaga tcgacatcta
     4681 cgacatctcg ctcgaacgta tcacccgcca gtacctcgaa tatctgcagg cgttcaaaga
     4741 actgaagatc gacgtcgccg gtgagtttgt cgcgatggcg gcgaatctca tttacctgaa
     4801 aagccgcagt ctgctcccgc gcgaccaaca accgcccgaa gaagatgccg aggaacatga
     4861 tccgcggtgg gacctgatcc ggcagttgat cgaatataaa aagttcaagg aagccgcggc
     4921 tgacttgaac gtgcgtgcgc tcgcacgaga gcggatgttc tcgcgccagg gcgatcccgc
     4981 ggcgacaccg gagcaaccat ttcgcctcga tgaggtcggc attttccagc tcatccacgc
     5041 cttccagaac gcgatcaagc gcgtcgaagc gcgccaaagt gtgcaggaga tcttcggaga
     5101 ccagtttacc gtctcggaga agatcgacat tatcctggaa cgtgtggcga atggcgcgac
     5161 gctgcgtttt tccgacttgt tcagcgcagt cgcttcgcgc gtggaggttg tcgtcacatt
     5221 tctcgcgctg ctggagttga tccgtttgca gcaagtgcac gccctgcagc ggaatttttt
     5281 cgacgagatc gaaatcgcgc cggcggtcgc ttgagtcaga tctgcaaccg atgccgcgcc
     5341 ttttgatcgc tggttgcggt tacgtcggcg ccgcgacggc ggatttgctt ttcacccgtg
     5401 gctgggaagt cgaaggttgg acacactcgc aagagtcagc cgggaaactt ggacggaaag
     5461 cttatcaagt ccacgccgtc gacctcacag agcgcgcgga cgtggcgaat tgctcgacca
     5521 atttcgacgc cgtgatccac tgcgcgagta cccacggcgg cggagcggaa ctatatcgac
     5581 ggctctatct cgatggcgcg cgcaatctcc tcgagcgctt cgcaggatcg acaatcctct
     5641 tcaccagcag cacgagcgtg tatgcgcaat ccgacagctc gtgggtgcat gaagaaagcg
     5701 cgactgagcc ggcgcccgag agtgggcgcg tccttttgca aacggaaaag ttggtgttag
     5761 cgcacggcgg catcgtcgcg cggctcgctg gaatttatgg gccgggccgc tccgttttac
     5821 tcgagaaatt tttgagcgcc gaagctttca tcgatccaga acacgaccgc ttcatcaacc
     5881 aggtgcatcg cgatgacatc gcca
//
LOCUS       DASTDG010000012         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_719530, whole genome shotgun sequence.
ACCESSION   DASTDG010000012 DASTDG010000000
VERSION     DASTDG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719530"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFO30_00390"
     CDS             <1..219
                     /locus_tag="VFO30_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417772.1"
                     /translation="RIRYGSKLDALDAFKARHGLLQDVWVVIHPVLVTLCQSTGDGGR
                     LLRTQPSFTKTRSPARTVAVAKRRTDHQ"
     gene            complement(284..1930)
                     /locus_tag="VFO30_00395"
     CDS             complement(284..1930)
                     /locus_tag="VFO30_00395"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET9417773.1"
                     /translation="MRPRRALNRLKLGPVVGHTDDTTSKVWIQVFDDPNDYKLRVAGA
                     GVFDFVSTEPAGLEFHTAIARATGLRSDWKYTYSIFRRGRRVVGGKGSFRTMPDPDSV
                     APILFCAVSCSTLATDGVWPQMAKFIKESQPSFLLMMGDQVYIDEDDPNIFELHYDSD
                     SATRRAALAEKYRLSWSRKVIQQTFANIPTYMVWDDHEIRDGFGSLACDSPTLVAQFS
                     RGAKMFARSDVYFQDCRDVYWHFQGCHNPLQGETAEPSLPNYIKDPPAGGPRRAMPFV
                     FRCGRVLILMTESRTERDVFRAEFPILGGEQWQFIDKVFANLPSEIETLVVATPTPIA
                     SIDPHGQVMKLMGDRTDDIEAFKRGDEKNVLDPHSSEDLGDLALAIAGHELTNLTGTP
                     VNLGAFKVSNLDEARDQWSHKFARPEQELMLRKAAQARFTNRNGSQGRELIFLSGDIH
                     VGCIFDITMLNPPYKIVSLTSSGISAKQEVKADLFVGTFVDEDFKIAGGIRSVLREIV
                     PDFNFGIVQVLQTGDGAEVNALLAHEGNSFAAGFDISKLI"
     gene            complement(1933..3831)
                     /locus_tag="VFO30_00400"
     CDS             complement(1933..3831)
                     /locus_tag="VFO30_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417774.1"
                     /translation="MADEDTLPPPLEIPWRLASTTLPFSNGEADQTSITLFTHIPDDD
                     VLTAKFPDERLVYIKITASISPASFPIFPPFPSGALGEGIPVYHMLLDLKVRKKSGET
                     GTIRPYFHAAVPLKRRMIQSGVVGHEIFEGEASGQFMGKSGSEMHEALNSESRTTSMS
                     ASLGVGVGIGPVSIGASGSVRTTSTDVTANRAVTQNVDTTTRQASDERRELVSHSTKV
                     ENILSLLNAKYVGTPFLSFSLNPRPLNLLSLDPADPNLWFSELLARRSSGVEGLQEFT
                     AVIVVPKDEDFCVSARLRRVCLFDAPPGPLTFDEHYDNSLAKLVRLLNYVERTFPIGT
                     PLEELDVDVLPALAPDKFPRPVINFWEIASIGGFLAPLIFAVSPSPTPVVTGVSTADV
                     VYKHPLEIWLDTLRDEYEQDVSRSPLERGVLLSETRTLDTCFAFSEGALNVSNTSASV
                     VPLAPLGINPAVIDIGGVRGGALDPNRDVRARGIEIVTRWNALEAQLANILNNRRTFP
                     KKQPKLNDISVVGVLVERWAKLQPQDPNNVDFNTAVSAIGLSAEHRRLLKSAGATDLR
                     SIARALQTAPTIESYNANLQTRQKRDRKARAVAKPISFAISATKADEMRRAISAALAK
                     SLEAKSEE"
BASE COUNT          848 a         1150 c         1248 g          843 t
ORIGIN      
        1 cgcatccgct atgggagcaa actggacgca ctggatgcat tcaaggcgcg gcatggtctg
       61 ctccaggacg tttgggtagt gatccatcct gtgctcgtca ctctctgcca atcgacgggc
      121 gacggcgggc gattattacg cacacagccc tcgttcacga agacgcgatc ccctgctaga
      181 actgtcgcag tcgcaaagcg gcgaactgat catcaataaa aacatcgccg tcgttggtcc
      241 gggtgcgaac cttcttgccg tcgcgcgcgg cgaaaaattg atttcagatc agtttggaaa
      301 tgtcgaagcc agccgcgaaa gaatttcctt cgtgcgcgag cagcgcattc acttctgcac
      361 cgtcaccagt ctgcaagacc tgcacgatgc cgaagttaaa atccggcacg atctcgcgta
      421 ggactgagcg gatgccgccg gcgatcttga aatcttcatc gacgaacgtg ccgacgaaga
      481 ggtccgcttt cacttcttgc ttagcgctga tcccggatga agttagcgaa acgatcttgt
      541 agggcggatt caacatcgtg atgtcgaaaa tgcagccgac gtggatgtcg ccggaaagaa
      601 agattagctc gcgaccctgg ctgccgttgc gattcgtaaa acgcgcctgc gctgctttgc
      661 gcagcatgag ttcctgctcg ggccgggcga acttgtggga ccactgatcg cgcgcttcgt
      721 cgagattaga gactttgaag gcgcctagat ttacgggtgt tccggtcagg ttggtcagtt
      781 catgaccagc aatggcaagg gcgagatcgc ccaggtcttc cgacgaatgc ggatcgagca
      841 cgttcttttc gtcaccgcgc ttgaaggcct cgatgtcgtc ggtgcgatcg cccatcaact
      901 tcatgacctg gccatgtgga tcgatcgacg cgatcggggt tggcgttgcc acgacgagcg
      961 tttcgatttc tgacggcaga ttggcgaaaa ccttatcgat gaattgccat tgttccccgc
     1021 cgagaatcgg aaactcagcc cggaacacgt cgcgttccgt gcgactctca gtcatcagga
     1081 tgagcacgcg tccgcagcga aagacgaacg gcattgcgcg acgcggtccg ccggcgggcg
     1141 gatctttgat gtagttcggc aacgacggct cggccgtctc accttgcagc gggttgtggc
     1201 aaccctggaa gtgccagtaa acatcgcggc aatcctgaaa atagacatcg gagcgcgcga
     1261 acatctttgc gccgcgcgag aattgcgcga ccaatgtcgg actatcgcac gcgagcgaac
     1321 cgaagccgtc gcgaatttcg tgatcgtccc agaccatgta ggtgggaatg ttggcgaagg
     1381 tttgctggat gactttgcgg ctccagctca gccgatattt ttccgcgagc gcagcgcggc
     1441 gcgtggcgga atccgaatcg taatgcaact cgaaaatatt cgggtcgtct tcgtcgatgt
     1501 aaacctggtc gcccatcatg agcagaaacg acggctgcga ttccttgatg aatttcgcca
     1561 tctgcggcca gacgccgtcg gtcgcgagcg tgctgcatga aaccgcgcag aaaagaatcg
     1621 gcgcgaccga gtccggatcg ggcatggtgc ggaaggaacc tttgccgccc accacgcgcc
     1681 gaccgcgacg aaaaatcgag taggtgtatt tccaatcgga acgcaaaccc gtcgcccgcg
     1741 cgatggcggt gtgaaattcc aggcccgccg gttccgtgct gacgaaatcg aacacgcccg
     1801 caccggcgac gcgcaatttg taatcgttcg ggtcgtcgaa aacctgaatc caaaccttgc
     1861 tggtggtgtc gtcggtgtgt ccgacaaccg ggccgagctt cagccgattc agcgcccgac
     1921 gcggacgcat gatcattcct cgcttttcgc ttcgagactc ttcgcgagcg cggcgctgat
     1981 cgcgcgtcgc atctcgtccg ctttcgtggc agagatggca aacgatatcg gcttcgcgac
     2041 cgctctcgct ttgcgatcgc gcttctgccg cgtttggaga ttcgcgttgt aactctcgat
     2101 cgtcggtgcg gtctgaagcg cgcgcgcgat ggaacgaagg tcagtcgcac ctgcagactt
     2161 gaggagacga cgatgttccg cgctcaagcc gattgcggag accgcggtgt tgaaatcgac
     2221 gttgttcgga tcctgcggct gcaatttggc ccagcgttcc accagcacgc cgaccacact
     2281 aatatcattc agcttcggtt gcttctttgg gaaggtgcga cggttgttca agatgttggc
     2341 gagctgcgct tcgagcgcgt tccagcgagt gacaatctcg atgccgcgtg cacgcacgtc
     2401 gcgattcgga tcgagcgcgc cgccgcgcac gccgccgatg tcgatgacgg cgggattgat
     2461 gccgagcggg gcgaggggaa cgacggaggc cgaagtgttg ctgacgttga gcgcgccttc
     2521 actgaaagcg aaacaggtat cgagtgttcg cgtttccgac aagagcacgc cgcgctcgag
     2581 cggtgagcgc gagacgtcct gctcgtattc atcacgtaac gtatcgagcc agatttcgag
     2641 aggatgtttg tagacgacat cggcggtgct gacgccggtg acgactggag ttggacttgg
     2701 tgagacggcg aaaatcagcg gcgcaagaaa tccgcctatg ctcgcgattt cccaaaaatt
     2761 gatgaccggg cgcggaaatt tatccggcgc caaagcgggc agcacgtcca catccagttc
     2821 ttccagcggc gtaccgatgg ggaaggtgcg ctcgacgtag ttgaggagcc ggaccagctt
     2881 cgcaagacta ttatcatagt gctcgtcaaa ggtgagcggg ccgggcggcg catcgaacag
     2941 gcacacgcgg cgcagtcgtg cgctcacgca aaagtcctca tccttcggca ccacgatgac
     3001 ggcggtgaat tcctgcaggc cttcgactcc gctggaacgg cgcgccagca gctcactgaa
     3061 ccagagattc ggatcggccg ggtctagcga gagcaaattc agtgggcgcg gattaagcga
     3121 gaaactcagg aatggcgtgc cgacatattt cgcattcagg agcgagagaa tgttttcgac
     3181 cttggtcgag tgactaacga gttcgcggcg ttcatccgat gcctgccgcg tggtcgtgtc
     3241 gacattttgg gtcacggcgc gattggcggt gacgtcagtg ctggtcgtcc gcaccgagcc
     3301 gctcgcgcca atgctgaccg gaccaatccc gaccccgacg cccagactcg cgctcatcga
     3361 agtcgtgcgc gattcgctgt tgagcgcttc gtgcatttcg ctgccgctct tgcccatgaa
     3421 ttgaccggac gcttcgcctt cgaaaatttc atggccgacc acgccgctct ggatcatgcg
     3481 gcgcttgagc ggcaccgcgg catggaaata cggacgaatc gttccggtct cacccgattt
     3541 cttgcgcacc ttgagatcga gcaacatgtg gtaaacggga attccttcgc cgagcgcgcc
     3601 acttgggaac ggtggaaaaa tcgggaacga tgcgggcgag atcgacgccg tgatcttgat
     3661 gtaaaccagc cgttcgtctg ggaatttcgc ggtcagaaca tcgtcatccg gaatatgggt
     3721 gaagagcgtg atcgaagtct ggtccgcttc gccgttgctg aaaggcaacg tcgtggatgc
     3781 aaggcgccac gggatttcca acggcggcgg aagagtgtcc tcatcagcca tggcgagttc
     3841 agcatacagt tcgaaggatt gatgtcatgt cgaatttgta gggaaaagcc tctgaataac
     3901 gtaaggtgta gccctacata atgtcggttt gttgccacaa ttcgacagcg gcggagagtt
     3961 gaacggggga ttcgttagcc gcctgcccag cgtctacgca ggtcgggtac aaaagtagcc
     4021 gcagcgcgct ccaccgggct gcggcaatcc acttcatggt agccgcggta tccggccact
     4081 aagtgctcg
//
LOCUS       DASTDG010000013        11442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1389936, whole genome shotgun sequence.
ACCESSION   DASTDG010000013 DASTDG010000000
VERSION     DASTDG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389936"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFO30_00405"
     CDS             <1..540
                     /locus_tag="VFO30_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9417775.1"
                     /translation="KVRDKSRMEDAKDELRGLMRRVRGLPPEKDDDFSINEQQALKTT
                     LDPIKNSIAIAGLFITGLSLFVGAIGIMNITFVSVKERTKEIGTRKALGARRRTILLQ
                     FLIESTALCLLGGLIGLAFAYLICVAIGAGFPAFPIRFSFNLVLISMIVSVLTGLLSG
                     FAPAWTASRLDPVTALRYE"
     gene            533..979
                     /locus_tag="VFO30_00410"
     CDS             533..979
                     /locus_tag="VFO30_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9417776.1"
                     /translation="MNRNYLTTESTETTKLEKGVNELTEIVIGCAIEVHRAFGPGLLE
                     SGYEICLCRELSLRNLPFECQKPLPLTYKGVNLDCGYRANVIVDRRVLVEVKSVDELA
                     PIHDAQLLSYLKLSGLKVGLLINFNVRVLSHGLRRKVLGPPEEISL"
     gene            1016..2251
                     /locus_tag="VFO30_00415"
     CDS             1016..2251
                     /locus_tag="VFO30_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417777.1"
                     /translation="MLFSEIVKMALSSLGANKLRAALTMVGITIGVFSVISVMTAIGA
                     LQSSIESGISFLGSNIFQFAKLPATITTSGAAQKKFVNRRNITYRQALRYAQLMEASA
                     REICLKSFEFKGQAVYHGMKTTPSLAVVGTNKSFLAANAYTLAYGRNLTDEDVEFARS
                     VLVVGKVIEKRLFPHESPIGKTIRLNGHNYTIVGVMAEKGTAFGQSQDDICAMPITRF
                     FENYGEANRTVNIATQSFSQETYNATLGKGIGAMRMARGLRANERDDFEIYSNDSLKS
                     AFANIAGIVRIGAFVISFIALLAAGIGIMNIMLVSVTERTKEIGVRKSIGARSRDILR
                     QFLAEAVFISEAGGLLGILLGVIGGDLLAAWLQANLIFPFGWALAGLIVCSAIGIGFG
                     LYPAYRAASLDPIEALRYE"
     gene            2359..4158
                     /locus_tag="VFO30_00420"
     CDS             2359..4158
                     /locus_tag="VFO30_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9417778.1"
                     /translation="MPGSLQKPAETTRPSVILIEEYDALCAALTAALRKFAPEHAIFV
                     AKSLREAESLAGKNPVDLFVIDADPPWPGISNFLKKMRAAHPNARGLVIGAAIPPEIA
                     AERGLAGALQFAEKPFELPNFGAAVQALLGPWREAGSAGIRGSLAALNPIDIVLLHYA
                     AGASVILDLHAGKTPYGEIQIYRGQVSHAEAGKLVGADALREILTSPELRMRERVGGA
                     IGQRTVAGWTKIVIDALRALPVSAPAASKAAALAKRIVAVDDTEMLLVFVQDVLTTAD
                     AALQVTTAKTATEGLERIRSVAPDLVLLDYDLPDFNGGELCRRLLQDERTASIPVIMM
                     SAHAAEMTAAAAQLGNIRATIEKPFFSKALVEVVRRTLTQPPRRKVEKAGPVKVAVAG
                     IAPRPETQPLSKTTPLEVPAIDQKQAVLGLFLDVIAMQFTPQLQMGSIRARPASTGIA
                     LQLARLQLRDSTPAEIGFQLAATELDATGRISLVRLRPSGKPFQGTQTRNVFQIGSVA
                     VIPADARQRVQLTPTGSAPMTVKLVAQLELAGIQLSQTFQVEQLALKWPSNIVRVTLN
                     PKAPDATGARFAITAVTLSEGNRLKELVLDPVK"
     gene            4272..4736
                     /locus_tag="VFO30_00425"
     CDS             4272..4736
                     /locus_tag="VFO30_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9417779.1"
                     /translation="MDEYPKLPPRLRWIFMHNPVFFVTCCTFQRRTLLASDAVHAAFV
                     AFATRAYRNNIAVGRYVIMPDHLHVFVCGDDDFELGRWIGMLKQAVAKHIGRSGSTPP
                     IWQRGFFDHLLRSAESYSQKWNYVRDNPVRAGLVAKPEDWPYSGEVVVIDRA"
     gene            complement(4806..6101)
                     /gene="purB"
                     /locus_tag="VFO30_00430"
     CDS             complement(4806..6101)
                     /gene="purB"
                     /locus_tag="VFO30_00430"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9417780.1"
                     /translation="MIDRYSRPEMRNIWSEERKFQIWLEIEILACEAMAELGQIPKAD
                     AAEIRKRARFSIPEILEIEKRTNHDVIAFLENVAESVGPAARWIHQGLTSSDILDTAI
                     AVQMMQSADLLLADLRALRNVVGDRAREFKVTPMIGRSHGIHAEPITFGLKLALMFDE
                     FGRAEERLTQTRERVRVGKISGAVGTHAYVDPGVERAVCERLGLKPATISTQIVQRDV
                     HAEFLTTLALIASSIDRWATEFRHLQRTEVLEVEEFFGQGQKGSSAMPHKRNPITNER
                     LSGLARVIRGNALVALENVALWHERDISHSSAERIILPDSCTLLDYMLVKLREVVAGL
                     QVYPERMQQNLELTKGLYFSQSILLALTRAGVERKAAYEAVQRAAMKTWKGEGTFPEN
                     VKQEPEIAAKLSGKEIDRLCSLDIHFRHIDGTFKALGLE"
     gene            complement(6218..6436)
                     /locus_tag="VFO30_00435"
     CDS             complement(6218..6436)
                     /locus_tag="VFO30_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417781.1"
                     /translation="MAPELLSKSTAPMKKNTLILKPRPKLSLGDLILAVSSCTKNTKE
                     TVATVADLLASGQVRVKSDGRFIKARVC"
     gene            complement(6542..7186)
                     /locus_tag="VFO30_00440"
     CDS             complement(6542..7186)
                     /locus_tag="VFO30_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9417782.1"
                     /translation="MHHLLDTWFVWVLTGGYTGIIALMAMESSIFPVPSEIVIPPAAF
                     LAAQGKLSFAGVIVAGTVGSYLGSTISYWAARLIGRPLIVKYGRFVLLSPKKLERAEH
                     WLARYEAGGVFFARLLPVVRHLISIPAGIVRMKFMIFSFMTIAGAAIWCWILAYLGDK
                     AYRLEPALLTEPEALMRFIRSQSTWILVVVLVFAVLYILTMRLSKPPENRSFDT"
     gene            7261..9003
                     /locus_tag="VFO30_00445"
     CDS             7261..9003
                     /locus_tag="VFO30_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9417783.1"
                     /translation="MSIYRRVLNYYRAFLPQTLLGLILSLCGIGLNLLKPWPFKMIVD
                     DFLGPNVASARDRQIWIPLLCTALVLIQLLWGVANWAMNYLFVKIGLQALVRLRTELY
                     AHLQRLSLKFHDTRRSADSSFRVAYDSQAIQTIYNKGFTNVFGSAITLLGTFLIMLRL
                     DWKLTLLSLAIVPLIVGTIFLFARRIRRESTYIQEQESALLAQAQEGLSSIRMVHAFG
                     REEFEVGQFQRHAYQSLHANLRLTLTNVNSALVISTLMVTGTAAMYYVGTLHVLGGTL
                     TLGSLLVFSAYLLMLYQPLESLTYTAWAMEGATAGARRCFEVLDRADDVLDAPDAIAI
                     TTTSGSVSFQNVSFAYTRDRHVLKDVDLAIAPNQIVGIVGGTGAGKSTLLSLVPRFYD
                     PISGSITLDGRDVRTITKKSLRAQIAIVLQDTLLFSTTVRENIAYGRADATDAEIVDA
                     ARRAEADEFIRELPNGYASPVGERGSHLSVGQRQRIGIARAFLKNAPILLLDEPTSAL
                     DPATEAAIIDTIKELMRDRTTLIATHRLAAIHQLAQIVVLERGRIVEKGRGNELIARG
                     GAYAQLYRSGRIAP"
     gene            9000..9587
                     /locus_tag="VFO30_00450"
     CDS             9000..9587
                     /locus_tag="VFO30_00450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9417784.1"
                     /translation="MSNVREDRHVQGDWWPQPVPSNVEFAEGFYCESAQIFRMLRNKQ
                     PGAVIIGKHVSCYAGCSFAVGENGRCTIGDFTLLNGALIMAEEKIDIGSYCLISWNVG
                     IADSDFHPLEPAQRLLDAQALAPFYASRPPRPKLGTAPVKICDNVWVGMNAIILKGVT
                     IGENSVVAAGAVVTKSVAPNTVVAGNPAVQVKTFL"
     gene            9703..10875
                     /locus_tag="VFO30_00455"
     CDS             9703..10875
                     /locus_tag="VFO30_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417785.1"
                     /translation="MKRKRIVVMGFMGSMPIAGVIWQHIHYIVGLQRLGHDVYYIEDS
                     ARLPYNPETFEINNEFDYAAQLLERVAGEFDFRGRWSFCARYLQGNPTAGLRLKRIRQ
                     LYCESDAILNICGAQELNDDIAEARRLIYVESDPGVEQIKVDKRVRSTVDYLNRHDAL
                     FTFGENVGTKTFPVPTHGLNWLPTRQPVVTDLWKTRHSPARAAVLTSIANWSTSGLKD
                     ITWRGDKYLWSKSREFLRFVAAPKRAGEPFELATNIKDAKMRAKFLRNGWRLRSPLQL
                     SVDYWRYRDYIRRSKGEFTVAKDQYVRLNTGWFSDRSACYLAAGRPVILQETGFTNNY
                     GNRAGLFGFGSLGEIAEAVQMINHDYGYHSRAARQLARELFEADMVLKSLLERASL"
     gene            10924..>11442
                     /locus_tag="VFO30_00460"
     CDS             10924..>11442
                     /locus_tag="VFO30_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9417786.1"
                     /translation="MKWCALISCVLLLVRSTAFSQQENNTPGPSASPPRFTGAGARSS
                     APPAPAIVPVPVAPAKPNGPVQKSLVRITATEVEPDYRAPWNAGALQRGIGAGFVIED
                     NRIMTNAHVVSNSRYLTVERDGDPNKYPARVLFVAHDCDLALITVDSPNFYKDMLPLK
                     FGGIPALESTVSA"
BASE COUNT         2409 a         3301 c         3283 g         2449 t
ORIGIN      
        1 aaggtgcgag acaaatcgag aatggaggac gcgaaggatg agctgcgcgg tctcatgcgg
       61 cgcgtgcgtg gactaccacc ggagaaggac gacgacttta gcatcaacga gcaacaagcg
      121 ctcaagacga cgctcgaccc gatcaagaat tcgatcgcga ttgccgggtt gttcattacc
      181 ggactttcgc tgttcgtggg cgccatcggc atcatgaaca tcacgttcgt gagcgtgaaa
      241 gagcgcacga aggagatcgg gacgcgcaaa gcgctcggtg cgcggcggcg gactattctg
      301 cttcagttcc tcatcgaatc cacggcgctg tgtttgctcg gtgggctaat cggcctggcg
      361 tttgcgtatc tcatatgcgt cgcgattggc gcggggttcc cagcatttcc gatccgcttc
      421 tcgttcaatc tggttttaat cagcatgatc gtttcggtcc tgaccggcct gctctccgga
      481 ttcgcgcccg catggacagc gtcgcgcctc gatcccgtca ccgcgctgcg ttatgaatag
      541 gaattattta accacagaga gcacggagac cacgaagctg gaaaaaggcg tcaatgagct
      601 gactgagatt gtgatcggct gcgcgatcga ggtgcatcgc gcattcgggc ctggcctgct
      661 tgaatctggt tacgagattt gcctgtgccg agaattgagc ctgcgcaatc tgcctttcga
      721 gtgccaaaaa cctcttccac ttacttataa aggtgtaaat cttgattgcg gctatcgagc
      781 gaatgtgatc gtggacaggc gcgttctggt cgaggtgaaa tcggtcgatg aactcgcacc
      841 aatccatgac gcgcaattgc ttagctactt gaaattgagt ggcctaaaag ttggtctgct
      901 catcaacttc aatgtgcgcg tgctgtcgca cggacttcgc cgcaaggttc tcggcccacc
      961 cgaagagatt tcgctctgaa ctccgtgtcc tccgtgctct ccgtggtgaa ttaagatgct
     1021 ctttagtgag atcgtgaaga tggcgctgag ttcgctcggg gcgaacaaat tgcgcgcggc
     1081 tttgacgatg gttgggatca cgatcggcgt gttttcggtt atctcggtga tgactgccat
     1141 cggggcgctg caaagctcga tcgagagtgg gatcagcttc ctcggttcga acatttttca
     1201 gtttgcgaaa cttccggcga cgatcacgac atctggcgcg gcgcagaaga agttcgtcaa
     1261 caggcgcaac attacttatc gccaggcgct gcgatacgca cagttgatgg aagccagcgc
     1321 acgcgaaatt tgcctgaagt ctttcgagtt caaaggccag gcggtttacc acggcatgaa
     1381 aacaaccccg agcctggcgg tcgtcgggac aaacaaaagc tttctcgcgg cgaatgccta
     1441 cacgctcgct tacggacgca acctgacgga tgaagacgtc gagtttgcgc gcagcgtgct
     1501 cgtggtcggc aaggtgatcg agaaacgatt gttcccgcac gaatcgccga ttggtaaaac
     1561 gatccggctg aacgggcaca attacaccat tgtcggcgtg atggcggaaa agggcaccgc
     1621 cttcggccaa agccaggacg atatttgcgc gatgccgatc acgcgcttct tcgaaaacta
     1681 cggcgaagca aatcggaccg tgaacatcgc gacgcagtcg ttctcgcaag aaacatataa
     1741 cgccacgctt ggcaaaggca tcggtgcgat gcgcatggcg cgcggcttgc gcgcgaatga
     1801 gcgggacgac ttcgagattt attcgaacga ctcgctcaag agcgcgttcg cgaatattgc
     1861 agggatcgtc cggatcggcg cattcgtgat cagtttcatc gcactgctcg cggctggcat
     1921 tggcatcatg aacatcatgc tggtgagtgt gacggagcgg acgaaagaga tcggcgtgcg
     1981 caaatccatc ggcgcgcgca gccgtgacat tctgcggcag tttctcgcgg aagcggtgtt
     2041 tatttccgaa gccggcggtc ttttggggat cctgctcggg gtgatcggcg gcgatctgct
     2101 cgcggcgtgg ttacaagcga atttgatttt cccattcggc tgggcgctcg ctggattaat
     2161 cgtctgttct gccatcggca tcggcttcgg tctctatccg gcctatcgcg cagcgtcgct
     2221 cgatcctatc gaagcgctgc gctacgagta acatcgaggt cagcgggccc atgcgcgcca
     2281 gtgcatggcg acccattcct cttttttttg acgcttcgcg cgcgcgtttg tcttacgctg
     2341 ggaactttcc actcgcacgt gcctggttcc ctccaaaagc ccgccgaaac gacgcggccc
     2401 agcgtcattc tcattgagga gtatgacgca ctttgcgctg ccctgacggc ggcgctgaga
     2461 aaattcgcgc ctgaacacgc tattttcgtg gcgaagtcat tgcgcgaagc ggaatcgctg
     2521 gccggcaaaa accccgtcga cttgttcgtt atcgatgcgg acccgccttg gccgggcatt
     2581 agcaattttc tgaagaaaat gcgagccgcg cacccaaatg cgcgcggcct tgtcattggc
     2641 gcagccatac ccccggagat tgcagccgag cgtggtttgg cgggcgcgct ccagttcgct
     2701 gagaaaccgt tcgaactccc aaacttcgga gccgcggttc aggcgctgct cggcccgtgg
     2761 cgcgaagcgg ggtcggcggg catccgtggc agcttggccg cgctgaatcc gatcgacatc
     2821 gtgctgttgc attacgccgc gggtgcgagc gtcattctcg atcttcacgc gggcaaaact
     2881 ccgtacggcg agatccagat ttaccgcgga caagtttcgc atgcggaggc tggaaaactg
     2941 gttggcgccg atgcgttacg cgagatcctc acctcacccg agctgcgtat gcgcgagcga
     3001 gtcggtggcg cgatcggaca gcgcaccgtc gcgggttgga cgaaaatcgt catcgatgcg
     3061 ttgcgcgcat tgccagtgag cgccccggct gcgtccaagg ctgcggcgct cgctaaaagg
     3121 attgtggcgg tcgatgacac cgagatgttg ctcgttttcg tacaggacgt actgacgacg
     3181 gcagatgcgg ccttgcaggt cacaaccgcg aaaaccgcga ccgagggatt ggaacgcatc
     3241 cgcagcgtcg ccccggacct tgttctgctc gactacgatt tgcctgactt caatggcggg
     3301 gagctttgcc ggcggcttct gcaagacgag cgtacagcca gcatccccgt tatcatgatg
     3361 tcggcgcacg cggcggaaat gaccgcggcg gcggcgcagc tcggaaacat tcgggcgacg
     3421 atcgagaagc catttttctc gaaagcgctc gtcgaggttg tgcgtcgcac tttaactcag
     3481 ccgccgcggc gcaaagtcga aaaagccggc cctgtcaaag ttgcggtcgc aggaatagcg
     3541 ccgaggcctg agacccagcc gttgtcgaaa accacaccgc ttgaagtgcc ggcgattgac
     3601 caaaaacaag ctgtgctcgg tttgttcctc gacgtaatcg cgatgcaatt taccccgcaa
     3661 ttgcaaatgg gctcgatccg cgcccgcccg gcgtccactg gtatcgcgct tcagttggca
     3721 aggttgcaac tgcgggattc caccccagct gaaatcggat ttcagctcgc cgcgacagaa
     3781 ctcgacgcaa ctggacgaat ctcgctcgtg cgcttgcggc cgagcggcaa accgttccag
     3841 ggaacgcaaa cacgaaatgt atttcagatc ggtagtgtgg cggttatccc ggccgacgcc
     3901 cgccaacgtg tgcaactgac gccaaccggc tccgcgccga tgacagtgaa gctggtcgcg
     3961 caacttgagc tcgccggaat ccagctatcg cagacatttc aggtggagca attggcgctc
     4021 aaatggccga gcaacatcgt ccgtgtgaca ttgaatccaa aagcgccgga cgcgacaggg
     4081 gcgcgttttg caatcactgc cgtgacgctc agcgaaggca accgcttaaa agagctagtg
     4141 ctcgacccag tgaaatagtc ccctgccgcg acgcgcgttg cgcacagcga agcgggtaca
     4201 ggacgtgtgc tgctcggagg gtaacgaatc ctgaattgcg cttcccgtac ggaagcggat
     4261 acaacgcttg cgtggacgag taccctaaac tccccccgcg tctcagatgg attttcatgc
     4321 acaatccagt gttctttgtc acttgctgta catttcagcg gaggacattg ctcgcgtcgg
     4381 atgcggtgca cgccgcattt gtagcgttcg caacgcgagc ctatcggaac aacatcgccg
     4441 ttggccgata tgtcatcatg ccggatcatc tgcacgtttt cgtctgcgga gacgatgatt
     4501 tcgagctcgg tcgctggatc ggaatgttga agcaagctgt cgccaaacac attgggcgat
     4561 cgggatcaac gccgccgatc tggcagcggg gcttcttcga tcatttgctg cgtagcgctg
     4621 aaagctacag ccagaagtgg aattatgttc gagataatcc cgtgcgcgca ggactggtag
     4681 caaagccaga ggactggccc tattcaggag aggtcgtcgt aatcgatcgc gcgtgactgt
     4741 agccgtcgcc ctgcgggcga cgtgcgcgct tcgcacagcg aagcggctac aattcatgcg
     4801 atcgttcatt cgagaccgag cgctttgaat gtcccgtcga tatgccggaa gtgaatgtcg
     4861 agcgagcaaa ggcgatcgat ttcctttccc gaaagtttgg ccgcgatctc cggttcttgt
     4921 ttcacattct cggggaacgt cccctcgcct ttccatgtct tcatcgccgc gcgctgcacc
     4981 gcctcatacg cggctttgcg ctccacaccg gcgcgcgtca gcgcaagcag gattgattgg
     5041 ctgaaataaa gtcctttggt cagctccaaa ttttgctgca tgcgctcggg atagacctgc
     5101 aagccagcaa cgacttcccg caatttcacc agcatataat cgagcaacgt gcacgagtca
     5161 gggaggatga tccgctccgc actcgagtgg ctgatgtcgc gctcgtgcca aagggccacg
     5221 ttttctaatg cgacaagggc gttcccgcgg atgactcgcg ccaggccact gagtcgttcg
     5281 tttgtaatcg ggttgcgctt gtgcggcatg gccgaactgc ccttctggcc ttgcccgaaa
     5341 aactcttcca cctcgagcac ttcggtgcgc tgaagatgcc ggaactcggt tgcccagcgg
     5401 tcgatgctcg aggcgataag cgccagcgtg gttagaaact cggcgtgcac gtcgcgctgc
     5461 acgatttgcg tcgaaatcgt cgccggcttt aaccccagcc gttcgcacac tgcgcgctcc
     5521 actccaggat cgacatatgc atgcgtaccg acggctccgc tgatcttacc cactcgcact
     5581 cgctcgcgcg tttgcgtgag gcgctcttcc gctcgtccaa attcatcgaa catcaacgcg
     5641 agttttagtc cgaaggtaat cggctctgcg tgaatgccgt ggctgcggcc gatcattggc
     5701 gtcaccttga actcgcgcgc ccgatccccc acaacgttgc gcaacgctct caggtcggcg
     5761 agtagaaggt cggcggactg catcatttgc acggcgatcg ccgtgtccag gatgtccgac
     5821 gaagtcagtc cttgatgaat ccagcgcgcc gccgggccga ctgactcggc gacgttctcc
     5881 aggaaagcga tcacatcatg gttggtgcgc ttttcgatct cgagaatctc tgggatggaa
     5941 aagcgtgcgc gcttgcgaat ttctgccgcg tcggcttttg gaatctgacc gagctcggcc
     6001 atcgcttcgc aggcgaggat ttcaatttcg agccagatct gaaacttgcg ttcctccgac
     6061 cagatattcc gcatctccgg ccgagaataa cggtcgatca cacggcgaag ataaaagcgg
     6121 cgagaagacg cggaaagcgt tttgtaccgc cgaaagacaa atcgctccgc cggtttacgg
     6181 ccggagcgat cagggaaatc tgcttgtcag tcttaggtta gcaaacgcgc gctttgatga
     6241 agcggccgtc gctttttact cgcacttgcc cgctggccaa aaggtctgcc accgtggcga
     6301 cagtctcttt ggtatttttc gtgcaggagc tcaccgcaag gatgagatcg ccgagcgaca
     6361 gcttgggacg gggtttgaga ataagagtgt tctttttcat cggagctgtg ctttttgaca
     6421 gcaattcggg tgccattgtt caatgtgaac aaggtggcca tcgtaggagg cgaagttgcc
     6481 gccaaattat gggactctta cgtgaccacg ccctgacact gatgttcgat tcgtgaacag
     6541 tttaagtgtc aaaagatcga ttctccggcg gcttcgagag gcgcatcgtc aagatgtaga
     6601 gtacggcaaa gaccagaacc actactaata tccacgtcga ttgacttcgg ataaatcgca
     6661 tgagtgcttc gggttcggtc aaaagcgcgg gctcgagccg atacgctttg tcgccgaggt
     6721 aagccagaat ccagcaccaa atcgctgcgc cggcgatcgt cataaaactg aaaatcataa
     6781 acttcatccg cacgatgcca gcggggatgg aaatgaggtg acgcaccacc gggagcaatc
     6841 gggcaaagaa aacgccgcca gcctcgtaac gagccagcca gtgctccgcg cgttccagtt
     6901 tttttgggct gagcagaaca aatcggccat attttacgat gagcggccgt ccaattagcc
     6961 gcgcagccca ataactgatg gtcgatccca aatacgatcc gaccgtgccc gccacgataa
     7021 cgccggcaaa actgagcttg ccttgcgcgg ccaggaacgc cgccggcgga atgacgatct
     7081 cgctcggcac gggaaagatc gagctctcca tcgccattaa cgcgatgatg ccggtgtacc
     7141 cgccggtcag cacccagacg aaccacgtgt cgagaagatg atgcatgctg attttcgatt
     7201 acgctggcca gcccgcgatt agttcaatcc aaaatctgca atgcgcaatc cctaattccg
     7261 atgtcgatct accgccgtgt cttgaattat taccgggcat ttctgccgca gactctgctc
     7321 ggtctcatcc tctcactttg cggtatcggc ttgaacctgc tcaaaccgtg gccgttcaag
     7381 atgatcgttg acgatttcct tggtccaaat gtggcatcgg ctcgggatcg gcaaatttgg
     7441 atccccctac tctgcactgc gctcgtcttg atccaattgc tctggggcgt agcgaattgg
     7501 gcgatgaatt atctgtttgt gaagatcggt ttacaagcgc tggtgagact acgcaccgag
     7561 ctctacgcgc acctgcaacg cctctcgttg aagtttcacg acacccgccg ctcggccgac
     7621 tcgagcttcc gcgtggctta tgattcccaa gcgattcaaa ccatctataa caaaggcttc
     7681 acgaacgtct ttgggtcggc aatcacattg ctcggcacct ttctcatcat gcttcggctc
     7741 gactggaagc ttactctcct ctcactagca atcgtgccac tgatcgtcgg cacgatcttt
     7801 cttttcgcac gtcggatccg acgcgaatcc acttacatcc aggaacagga gagcgcgcta
     7861 ctcgcgcaag cgcaggaagg actcagctcc atccggatgg tgcatgcctt cgggcgcgaa
     7921 gaattcgagg tcggtcagtt tcaacggcac gcgtaccaaa gcctgcatgc gaacttgcgg
     7981 ctcaccctta ccaatgtgaa cagcgcgctt gtcatcagca ctctgatggt cactggcaca
     8041 gctgccatgt attacgtcgg cacattgcac gtcctgggtg gaacgctcac gctgggatcg
     8101 ctgttggtct tcagcgccta tcttttgatg ctctaccaac cgctcgaatc gctcacttac
     8161 actgcatggg caatggaagg tgcaacggcc ggtgccagac gctgtttcga agtgctcgac
     8221 cgagccgacg acgttctcga cgcacccgac gcgatcgcaa tcacgacaac cagcggctcg
     8281 gtcagttttc aaaacgtcag cttcgcttac acccgagatc gacacgtgct gaaagatgtc
     8341 gatcttgcaa tcgcgccgaa tcaaatcgtc ggaatcgtcg gcggtacggg cgcgggtaaa
     8401 agtacgttgc tgagcttggt tccgcgattt tacgatccga tcagcggatc aataacgctc
     8461 gacggccgcg atgtgcggac gatcacgaag aaatcgttgc gcgcgcaaat cgcgattgtt
     8521 ctgcaagaca cgctgctttt ctccacgacc gttcgcgaaa atatcgctta cggccgagcg
     8581 gatgcgacgg acgcggaaat tgtcgatgct gccaggcgcg ccgaggcgga tgagttcatt
     8641 cgcgaactgc cgaacggata cgccagccca gtgggcgagc gcggcagtca tctcagcgtc
     8701 ggtcagcgac aacggatcgg catcgcacgc gcgtttctga aaaacgcacc gattctgctc
     8761 ctcgacgaac cgacctcggc gcttgatccg gcgaccgaag ctgcaatcat cgacacgatc
     8821 aaggagctga tgcgcgaccg gacaacgctg atcgcgaccc atcggctcgc cgcaatacac
     8881 cagctcgccc aaatcgtcgt gctggagcgc ggccgtatcg tggagaaagg gcgcggcaat
     8941 gaattgatcg cacgcggcgg agcttacgcg cagctttatc gctccgggag aatcgcgcca
     9001 tgagcaacgt gcgcgaagat cgacatgtgc aaggcgactg gtggccgcag cccgtgccgt
     9061 caaatgtcga gttcgccgag ggcttttact gcgaaagcgc gcagatcttt cgaatgttgc
     9121 gcaacaagca accgggggcg gtgatcatcg gcaaacatgt ttcctgttat gcgggctgct
     9181 cgttcgcggt gggtgagaat ggccgttgca caatcggcga tttcaccttg ctcaacggcg
     9241 cgctcatcat ggccgaagag aagatcgata tcggctccta ctgtctcatc tcatggaatg
     9301 tcggtatcgc cgattctgac tttcatccgc tcgagccggc gcaacgtttg ctcgacgcgc
     9361 aggcactcgc gccgttttat gcgagccgcc cgccccgccc gaaattggga accgcaccgg
     9421 taaaaatctg cgacaacgtg tgggttggaa tgaacgccat cattctcaaa ggcgtgacaa
     9481 tcggggagaa ctccgtcgtc gcagcaggtg ccgtggttac gaagtcagtc gccccgaaca
     9541 cggtcgtcgc tggaaatccc gcagttcagg tcaagacgtt cctgtgagac gagtagaaaa
     9601 cgccgaacgc ccaacgccca atcccgagtc tcaaattcag acaaactcct tctgcattcg
     9661 gcattgggcg ttcggtgttc ggcgttcgga gttttctttt tcgtgaaacg caagcgcatc
     9721 gtagtcatgg gcttcatggg ctcgatgccg atcgccggcg tgatctggca acacattcac
     9781 tacatcgtcg gtctgcagcg gctcggccac gatgtttact acatcgaaga ttccgcccgg
     9841 cttccctaca accccgaaac cttcgagatt aacaatgagt tcgattacgc cgcccaattg
     9901 ctcgaacgcg ttgcaggtga atttgatttc cgaggccgct ggagcttctg cgctcgctac
     9961 ttgcaaggca atccgactgc cggtcttagg ttaaaacgca tccgacagct ttattgcgaa
    10021 tcagacgcca tcctgaacat ctgcggtgcg caggaattga acgacgacat cgccgaagcg
    10081 agacgcctca tttatgtcga aagcgaccct ggcgtcgagc agatcaaggt cgacaagcgc
    10141 gtaagatcga cagtcgatta tctcaaccgc catgacgcgc tgttcacttt cggagagaac
    10201 gtcggcacaa aaacgtttcc ggtgccgacg cacgggttga actggctgcc gacgcgacaa
    10261 cctgtggtga cggatctgtg gaagacgcgt cactcccctg cccgcgctgc ggttttgacg
    10321 tcaattgcca actggtcgac gagcggcctt aaagacatca cgtggcgggg cgacaaatat
    10381 ctctggagca aatcgcgcga gtttctccga tttgttgcgg cgccgaagag agccggtgag
    10441 ccattcgagc tggcgactaa catcaaagat gcaaagatgc gcgcgaaatt cctgcgcaac
    10501 ggctggcgtc tgcgctcgcc gctgcaactg agcgtcgact actggcgtta tcgtgattac
    10561 atccgccgtt ccaaaggcga attcaccgtg gccaaagacc agtacgtccg cttaaacacc
    10621 ggctggttta gtgatcgcag cgcctgctac ctcgccgctg gccggccggt cattctccag
    10681 gagacaggtt ttacaaacaa ctacggcaat cgagccgggc tgtttggctt cggttcgtta
    10741 ggcgaaatcg ccgaagcggt gcagatgatc aatcacgatt atggatatca ttcgcgcgcc
    10801 gcgcgacagc tcgcgcgcga acttttcgaa gcggacatgg tgctcaaatc gctgctcgag
    10861 cgcgccagcc tgtgatccga tttgcttttt cgaaactgcg tttatcttct cggactttgc
    10921 ggcatgaaat ggtgcgccct gattagttgc gtccttcttc ttgtaagatc gacagcattc
    10981 tcccagcagg aaaacaacac gcctggacct tcggctagtc cgccgcgttt tacgggcgcg
    11041 ggcgcgcgct cgagcgcacc gccggcgccg gctatcgttc ctgtccctgt cgcgccagca
    11101 aaaccgaatg ggccggtgca gaagagcttg gtccgaatta ccgcgacgga agttgagccg
    11161 gattatcgcg cgccgtggaa cgcaggcgcg ctgcagcgtg gcatcggcgc cggtttcgtc
    11221 atcgaagaca atcgcatcat gaccaacgcg catgtcgtga gcaacagccg ctatctcacg
    11281 gtcgagcgcg acggtgaccc aaacaagtat cccgcgcgcg tcctgtttgt ggcgcacgac
    11341 tgcgacctgg cgctaatcac ggtggactca ccgaatttct acaaagatat gctgccgctg
    11401 aagttcggcg gcatcccggc gctggaatcg accgtatcgg ct
//
LOCUS       DASTDG010000014         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1272696, whole genome shotgun sequence.
ACCESSION   DASTDG010000014 DASTDG010000000
VERSION     DASTDG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272696"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFO30_00465"
     CDS             <1..405
                     /locus_tag="VFO30_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417787.1"
                     /translation="FDRNLRFGWWSLLVFLSLGGLLEIFHGLKIGWYVDVGNDMRRLM
                     FTLAHAHGTALALVNIAAGLTARSMKGLIGRSSVSFGLIWAGILFPLGFFLGGVVTYG
                     GDPGLGIWLVPIAALLLFYSVLRLARDLSKFR"
     gene            complement(402..1217)
                     /gene="lpxI"
                     /locus_tag="VFO30_00470"
     CDS             complement(402..1217)
                     /gene="lpxI"
                     /locus_tag="VFO30_00470"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978206.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET9417788.1"
                     /translation="MAQAPLHTLGIIAGNGVYPQVLAEAARKSGVRKIVMAAFTNETN
                     PAISQYVDTIEWLRVGQLSKLLKFFRVQNVHEAIMAGQIGPKNLFDLRPDVKALVVLA
                     KLKERNAESIFAAIADELRKVDVDLLPATMFLEDYLAPVGLIAGARLSPREEDDVDLG
                     WRIAKDIARLDIGQTVLVKNGTVLAVEAFEGTNEAIRRGGRLARKGAVMIKVAKPNQD
                     MRFDVPVIGTDTIRAAAEANLRVIAIEAGKTLLLEGHAIVGLVQAAGISLLGR"
     gene            complement(1275..1685)
                     /locus_tag="VFO30_00475"
     CDS             complement(1275..1685)
                     /locus_tag="VFO30_00475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET9417789.1"
                     /translation="MATLKLEIVTPEAKIYSEDVNMVTLPGSEGELGVYPKHVPLLTT
                     LKPGELRVVKDGRETALAVGEGFVEIRADAVSILTDMALEAEKIDIEAAEKAVERAQA
                     AMKEDLGAEQVAAIQASLQKALAQLHVKRRRHHG"
     gene            complement(1722..>2820)
                     /gene="atpD"
                     /locus_tag="VFO30_00480"
     CDS             complement(1722..>2820)
                     /gene="atpD"
                     /locus_tag="VFO30_00480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9417790.1"
                     /translation="GPVKFTTRYPIHRPAPELTEQDTKVQILETGIKVIDLVCPFSKG
                     GKVGAFGGAGVGKTVIIQELINNIAMKHGGVSMFAGVGERTREGNDLYREMIEAGVIN
                     EKDLGQSKVGMVYGQMNEPPGARLRVGLAALAMTEYFRDERNQDVLLFIDNIFRFSQA
                     GSEVSALLGRTPSAVGYQPTLAAEMGDLQERITSTTRGSITSFQAVYVPADDLTDPAP
                     ANTFAHLDSTIVLERSIAELGIYPAVDPLASTSKSLAPEVVGEEHYDVARGVQRVLQR
                     YKDLQDIIAILGMDELSPEDKLTVYRARKIQRFLSQPFHVAEVFTGHKGQYVSIQETV
                     RGFGEILEGKHDDVPEQNFYMKGNIDMIKES"
BASE COUNT          518 a          829 c          795 g          678 t
ORIGIN      
        1 ttcgatcgca acctgcgttt cggctggtgg tcgctcctgg tttttctttc gttaggcggg
       61 ctcctcgaaa tttttcatgg gctcaaaatc ggttggtatg tcgatgttgg aaacgacatg
      121 cgccgactga tgttcacgct cgcgcatgcc catggaactg cgctcgctct agtcaacatc
      181 gccgccggtt tgaccgcccg ctcgatgaaa ggattgatcg ggcgctcgtc cgtttccttc
      241 ggtttgattt gggccggaat tctttttccg ctgggctttt ttctcggcgg agttgtcact
      301 tacgggggcg atccgggtct gggcatctgg ctggtgccga tcgcggcgct gcttctcttt
      361 tacagcgtgc tgcgtctcgc gcgcgatttg tcgaaatttc gctaacgacc aagcagcgaa
      421 attccggcgg cctgcacaag accgacaatc gcgtggccct cgagcaagag agtctttcca
      481 gcttcaatgg cgatgacgcg tagattcgct tcggccgcgg cgcgaatcgt gtcggtgccg
      541 atcaccggca cgtcaaagcg catgtcttgg ttcggtttcg cgaccttgat catgaccgcg
      601 cctttgcgcg cgagcctgcc accgcgtcta atcgcttcat tcgttccttc gaaagcttcc
      661 accgccagca ccgttccatt tttcacgagc acggtttggc cgatatcgag ccgcgcgatg
      721 tctttcgcta ttctccagcc aagatcgaca tcgtcttctt cgcgcggcga caatcttgct
      781 ccggcgatta atccgaccgg cgcgagatag tcttccagaa acattgtcgc gggcaaaaga
      841 tcgacatcaa ctttccggag ctcgtctgcg atcgccgcga aaatcgattc cgcgttgcgt
      901 tctttgagtt tcgcaagcac aaccagcgct tttacgtctg ggcgcaaatc gaagagattc
      961 ttcggcccga tttggccggc catgatagcc tcatgaacat tttgtacgcg aaagaatttg
     1021 agaagcttgc ttaattgacc gacgcgcaac cattcgatgg tatcgacata ttgggaaatg
     1081 gcggggttgg tttcgttcgt gaacgccgcc ataacgattt ttctgacgcc ggatttgcgc
     1141 gcggcctcag ccaggacttg tggataaacg ccattgccag cgatgattcc gagagtgtgt
     1201 agcggtgcct gtgccaagca ccgaatctga aataaacagg cgcttggcac aagcgcctct
     1261 acaacatcgc cgccctaccc gtgatgccgg cgccgtttga cgtgcaactg ggcgagcgct
     1321 ttttgcagcg acgcctgaat ggccgcgact tgttccgcac cgagatcttc cttcatggcc
     1381 gcttgtgcgc gctcgaccgc tttttcggcc gcctcgatgt cgatcttctc ggcctcgaga
     1441 gccatatcgg tcaatatcga cacggcgtcg gctctgatct cgacgaagcc ctccccaacg
     1501 gcgagcgcgg tttcgcgtcc atccttgacg acgcgcagct cgcccggctt caacgtcgtg
     1561 agcaacggaa cgtgctttgg ataaacaccg agctcgcctt ctgatcccgg caacgtgacc
     1621 atgttcacgt cctcggagta aatcttcgcc tcgggggtga cgatctcaag cttcagtgtc
     1681 gccatggttg ttccgctgta acggtgtaac gcttcaacgg atcacgattc tttgatcatg
     1741 tcgatgttgc ccttcatgta aaagttttgt tcgggcacat cgtcgtgttt gccttccaga
     1801 atctcgccga acccgcgcac tgtctcctga atcgacacgt attggccttt gtggccggtg
     1861 aagacttcgg caacgtggaa cggttggctg aggaagcgct ggatcttgcg tgcgcggtag
     1921 accgtcagtt tgtcctccgg gcttaactcg tccatgccga ggatcgcgat gatgtcctgc
     1981 aaatctttgt agcgctgcag cacgcgttgc acgccacgcg cgacatcata gtgctcctca
     2041 ccgaccactt ccggcgcgag cgatttggag gtcgaggcga gtggatcgac ggcggggtaa
     2101 atgccgagct cggcgatcga gcgctcgagc acaattgtcg aatccagatg cgcaaacgtg
     2161 tttgctggcg ccggatcggt caggtcgtcc gccggaacat agacagcctg gaacgaagta
     2221 atcgaacctc tggtggttga tgtaatgcgc tcctgcaaat cgcccatctc cgcggccaac
     2281 gtcggttgat aaccgaccgc gctgggcgtg cggcctaaca aggccgagac ttccgaacct
     2341 gcctgcgaaa agcggaagat attgtcgatg aagagcaaca cgtcctggtt gcgctcgtcg
     2401 cggaaatatt ccgtcatcgc gagcgcggcc aggccgacgc gcaagcgcgc gccgggcggc
     2461 tcgttcattt ggccgtaaac cattcccact ttcgactgcc cgagatcttt ctcgttgatg
     2521 acgccggctt cgatcatttc gcggtagaga tcgttgcctt cgcgtgtgcg ctcgcccacg
     2581 ccggcaaaca tcgagacgcc gccgtgtttc atcgcgatgt tgttaatcaa ctcttgaatg
     2641 atgacggtct tgcccacacc ggcaccgccg aaagcgccga ccttgccgcc ttttgagaac
     2701 gggcagacga gatcgatcac tttgattccc gtctcaagga tttgcacttt cgtgtcctgc
     2761 tcggtcagct cgggcgccgg acggtggatc gggtaccggg ttgtgaattt gaccggcccg
//
LOCUS       DASTDG010000015         8465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_970831, whole genome shotgun sequence.
ACCESSION   DASTDG010000015 DASTDG010000000
VERSION     DASTDG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970831"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFO30_00485"
     CDS             <1..316
                     /locus_tag="VFO30_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417791.1"
                     /translation="LAPNPRVLLMDEPFAALDALTREQFYGALQKIWKERKKTIVFVT
                     HNVREAACLGDRVLLFSPHPGRLQEEFIVDLPRPREMNTSELATYAAEIMHSLKQSAA
                     TA"
     gene            313..1077
                     /locus_tag="VFO30_00490"
     CDS             313..1077
                     /locus_tag="VFO30_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417792.1"
                     /translation="MRRFLSAAGFFLLLILVWQWAFQAKLWSPVLLPSPVQVASYLKE
                     ASGDGTLVRSTVITMRRLLTGYFFGIVVGLPLGLLTARWMLVRDTLGTTALGLQTLPS
                     VCWVPLALLWFGQTEAAMLFVVIMGTLWSVIIATETGVRNVPPIYPRAALTMGSKRLH
                     IWLKVILPASLPFIVSGMKQGWAFAWRSLMAAEIYVTILTGFGLGQLLHFGREQQAME
                     QVIGIMIVIVLIGLLVDKTLFSPIERFLHRRWGTNE"
     gene            1074..1355
                     /locus_tag="VFO30_00495"
     CDS             1074..1355
                     /locus_tag="VFO30_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9417793.1"
                     /translation="MNRHAPSVSSCEITITNPLGLHARPAAQFVKHALRFRAEVWIVK
                     DGQRYSASSLIEILRANLDQDSTAILEASGRDADEALASLCQLIREFKE"
     gene            complement(1365..2252)
                     /locus_tag="VFO30_00500"
     CDS             complement(1365..2252)
                     /locus_tag="VFO30_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HET9417794.1"
                     /translation="MRVWKFVLPLVLLCSVASGFESRLPFKTIFKGGERFGRLVALAK
                     ANNWKSVPIGQRAAAVGQSLVGTRYKSFTLEIDNRIESPSVNFNGLDCWTFFETSLAF
                     ARMLNESEENWTPATLLRYIETDRYRSGECTGDYLSRLHYLEDWLYDNDRRGLVEDLT
                     PRLGGVSVPHSAREMSAGWRHYRYLAANRSLLGPLVRMEAQVSSRPLYQIPKSRVAKI
                     EPKLRSGDIIGIVSRDRRGLCSTSHVGLALRTTDGVLHFMHASSPSNYGRVVIDSRLS
                     DYLNRYRTDSGILVARPVK"
     gene            2359..3315
                     /locus_tag="VFO30_00505"
     CDS             2359..3315
                     /locus_tag="VFO30_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9417795.1"
                     /translation="MPIPFSRKSKRNVVRQSSDNGAEVRKSTRVKLRPRRDWHKRSYL
                     AEVLIPSLFIRRAGDYVPPEFPLIELGQLCLTWIGHASFLIQTREMNILIDPNWAKWL
                     KVIKRLKRPGFEIHHLPEIDVVLVTHAHFDHLDRRTLRKVAADQPIVVPVGVGNLVHD
                     LGFHIVHELDCWQTVQLGPIKVSLTPCYHWGARFLADLHRGFGGFAIELEGRTIFHCG
                     DSAFFPGFREIGEHYKIDIALLPIGAYDPPTGREVHMNPEEAVRAFLELEAQTFVPMH
                     YGTFRLGYEPLHEPLERLLAAARTHGIEEKIVVMTEGRPVVL"
     gene            3426..5696
                     /locus_tag="VFO30_00510"
     CDS             3426..5696
                     /locus_tag="VFO30_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417796.1"
                     /translation="MSRSRHTRSDIVGAVAAGTAVLVFCLLLLWHDPLVFWNDDYELS
                     VLPVFADVARSWSEGHWPLLSPYSWVCNNLAGEFQYGTFSVFVNAAVVLIWKFPLSFA
                     QQAAAISIIHLVVLAIGALLLARGRALSIPLSIFVAIIAALNGWIICWGATDWFGALG
                     AFAWLPWAWWACERALKGASASSRCSENNHRQDADATRSRWRFLWPAPFVYLVITGGF
                     PYTVLMLALVVAWLSIKSLGETKQWMSVLPMLVGVALGAGLAAPAWLALLDYVHGSAR
                     QLQTASAHWQWIVPWRALPGLVLPAWTVNWPDFSTRYLPHAATELACGLVAPSALIAG
                     FIWRPKLLVRHLKWELLLLLLVLLLAMLPTAGVFRWSFRWLPLFHLVLAICGAEVLRL
                     RPGSPTGSASVLLLILVTIAVLVFNTAGKYGLAVMFVYYEIAVIWVVGEWFLRDRTFH
                     EWAPAVVTFSILLATYICIPPNCGVPKYNFSQKLLSPSPLDPQRLYLSIYPPTEHTYR
                     VEKKPQPVGQIVRPGSTSMWATLRFVNGYSPILAAGVAREFNFAIHGEIDPAMGDYLL
                     SDRSGLLQQLGIDGLIIAQEIATNPASPDWQLVFSNNEGRVFHWRGGPLPRVRSVNEI
                     DSHSDERFVTAAISRIDDSRNRVELDVDVPSGKNAALLEFSRPYFRGYVARLGNINLA
                     VDSYRGLIPLVKVPPGSHGRLILTYRPAWLVYGGALSILCAAIFSVGVVAAAVTGGRT
                     KNNVELPRETAAATSL"
     gene            complement(5693..6862)
                     /locus_tag="VFO30_00515"
     CDS             complement(5693..6862)
                     /locus_tag="VFO30_00515"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HET9417797.1"
                     /translation="MLAPIAPPKPAAPASPWTRANPFPARLLVNRRLSAPESQKDTRH
                     FEIDLTGWGLSFEPGDSVAIYPTNDPELVNEIIRLLRATGDEPVPAAKMTKPFREALL
                     RDYSITQPTPKFLKAIAGRASAAPMLKELLHPERKHDLENYLWGAEVIDFLLDHPSAR
                     FTPAEFVGLLTKLQPRLYSVASSLRVYPDQVHLIVDVVRYETRGRLRKGVCSSFLAER
                     ADDVPVPVYPSLAKHFHMPEDRSLPLIMIGPGTGIAPFRAFLQERQVQGAKGKNWLFF
                     GAQREKCDFAYKEDWERFTRDGILTRFDCAWSRDQTHKIYVQHKILENAAELWNWIDR
                     DGAYFYVCGDAKRMAKDVDAALRKIVQEHGGKSVEEANQYVEKLKTEKRYKRDVY"
     gene            6954..8357
                     /locus_tag="VFO30_00520"
     CDS             6954..8357
                     /locus_tag="VFO30_00520"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9417798.1"
                     /translation="MPERTPNQFEPPSEHAPPVAAVPPLPRHDPYAAFRSRGFSFFTA
                     GNLLSLTGRQMLAVAVEWEIYRRTHSATALGLAGLVFAIPIVGLSLPAGHLADRISRK
                     QIILVAQIFSAFTSATLAIVSWQHLAIPQLSILRACSHGLNEIARVFERHNPQFHFDD
                     ASIPLIYLLLFIGAVARTFSWAARSSFFPTLVPRDAFSNAVTWNNSIFQIASVVGPTI
                     SGFLVAHIGFPIVYLIDAVTAFAFFLLVLPIPRAKQARDRTGQRTWRSLLAAMKFVFN
                     KKVILATITLDLFAVLLGGATALLPIFADQILRCGPVGLGWMRAAPAVGAFAMAMVVA
                     YLPPMRQAGRTLLWCVTGFGVATTAFGLSKALWFSLSMLFVAGALDSVSVIVRGSIVQ
                     LVTPDQMRGRVSAVNNIFIGTSNEFGALESGLTAALFGPVISVVAGGIGTILVVVASA
                     LRWPEIRKIGRLDKTLR"
BASE COUNT         1588 a         2571 c         2395 g         1911 t
ORIGIN      
        1 gctcgctcca aatccacggg ttctgctgat ggacgaaccg ttcgccgccc tcgacgcact
       61 gacgcgcgaa cagttctatg gagctctgca gaaaatttgg aaggagcgca agaagaccat
      121 cgtcttcgtc acacataatg tgcgcgaggc cgcgtgcctg ggtgatcgcg tgctgctttt
      181 ttcgccgcat cccggacgac tccaggagga gttcatcgtc gatcttccac gcccgcgcga
      241 gatgaatacg agcgaactcg ccacctacgc ggccgaaatt atgcattcgc tgaaacaatc
      301 ggccgcgacg gcatgagacg attcctttct gcggcgggat ttttcctgtt gctcatctta
      361 gtttggcagt gggcttttca ggcaaagctc tggtccccgg tgttgctccc ctcgcccgtg
      421 caggttgcga gctatctgaa agaagcaagc ggcgacggga cactcgtgcg ctcgaccgtc
      481 attacgatgc gccgtctgct caccggatat ttctttggca tcgtcgttgg tctgccgctc
      541 gggttgttaa ccgcgcgctg gatgctggtg cgcgacacat tgggcactac ggcgctggga
      601 ctgcaaacac tcccgagcgt ctgctgggtg ccgctggcac tgctctggtt tggtcaaacc
      661 gaagccgcca tgttattcgt cgtgatcatg ggaacactct ggtcggttat catcgcgacc
      721 gaaactggcg tgcgcaacgt gccgccgatc tatccgcgcg ccgcactaac gatgggttcc
      781 aagcgattgc acatttggct gaaggtgatt ttgcccgcgt cactgccatt tatcgtcagc
      841 ggaatgaaac agggctgggc tttcgcctgg cgctcgctca tggccgcgga aatttacgtc
      901 actattctca ctggattcgg actcggacag ctcctccatt ttggccgcga acaacaggcg
      961 atggagcaag tcatcggcat catgattgtc atcgtactga tcggcctgct cgttgacaaa
     1021 acgctttttt cgccgatcga acgattcctt caccggcgct gggggacaaa tgaatgaacc
     1081 gacacgcacc atcggtatcc agttgcgaga tcaccattac caatccgctc gggctccacg
     1141 cccgtccggc cgcgcaattc gtcaagcacg ccctgcgttt tcgcgcggaa gtgtggatcg
     1201 taaaagacgg gcagcgttac tccgcttcca gcttgatcga gatattgcgc gcgaatctcg
     1261 accaagactc gaccgcgatc ttggaagcaa gcggacgcga tgccgacgaa gcactcgcga
     1321 gcttatgtca gcttatccgc gaattcaaag aatgacctgc gcaattactt gaccggacgc
     1381 gcgactaaaa ttcccgaatc ggtgcggtag cggttcaggt aatcggagag ccgactgtcg
     1441 ataactactc ggccgtaatt gctcggcgat gatgcgtgca tgaaatgcag cacaccgtcc
     1501 gttgtgcgca gcgccagccc gacatgcgag gtcgaacaca gcccacggcg atcgcgcgag
     1561 acgataccga taatgtcgcc gcttcgcagc ttcggctcaa ttttcgcgac acggctcttt
     1621 ggaatttgat acaagggccg cgacgaaact tgcgcttcca tgcgcacgag cggcccgagc
     1681 aacgaccgat tggcagccag ataacgataa tgtcgccagc cggccgacat ctcgcgcgcc
     1741 gagtgcggca cgcttacacc gccgagtcgc ggcgttaaat cttcgaccaa cccgcgccgg
     1801 tcgttgtcgt aaagccagtc ctcgaggtaa tgcagccgcg acagataatc gcccgtacat
     1861 tcgccgctgc ggtaacggtc cgtctcgatg tagcgcagca acgtcgccgg cgtccagttc
     1921 tcctccgact cattcaacat gcgggcaaag gcaagtgacg tttcaaaaaa cgtccagcaa
     1981 tcgagcccgt taaaattcac tgagggcgac tcgattcgat tgtcgatctc gagcgtgaac
     2041 gatttgtagc gcgtgccaac gagcgactgc ccgactgcgg ccgcgcgctg accgatcggc
     2101 accgatttcc aattgttggc cttggccagc gcgacgaggc gaccaaaacg ctcgccgcct
     2161 ttaaaaattg ttttgaaggg caaccgcgac tcaaaaccgc tcgcgaccga gcaaagtaaa
     2221 accagtggaa gaacgaattt ccaaaccctc atgacccgcc aaacagaagc tcgaaatgta
     2281 ccctcgatca acttcctcac ctcgtccgct cttgaaagat tttgcgtcac tgctctaggc
     2341 ttatatgctt cgccgaacgt gccgatcccc ttctcgcgga agagcaaacg caatgttgtc
     2401 cgacaatcgt ccgataacgg cgccgaggtc cgcaaatcga cccgcgtcaa actgcggccg
     2461 cgccgcgact ggcataagcg cagctatctt gccgaggttc tgatcccgtc gctcttcatc
     2521 aggcgcgctg gcgattacgt ccctccggaa tttcctctga tcgagctggg tcaactttgc
     2581 ctcacctgga tcggccacgc ttcgttcctg atccagacgc gcgagatgaa cattctcatc
     2641 gacccgaact gggcgaagtg gctcaaagtc atcaagcggc tgaagcgacc cggcttcgag
     2701 attcaccacc ttccggagat tgatgtggtg cttgtcacgc atgcgcactt cgatcacctc
     2761 gaccgccgca ccttgcgcaa agtcgccgcc gaccagccga tcgtcgtccc ggtcggagtc
     2821 ggcaatttgg tgcacgatct cggtttccac atcgtgcatg aactcgattg ctggcaaacg
     2881 gtgcaacttg gacctatcaa ggtctcgctc acgccttgtt accattgggg cgcgcggttc
     2941 ctcgccgatc tgcaccgcgg cttcggcggg tttgccatcg aactggaagg ccgcaccatt
     3001 tttcattgcg gcgacagcgc gtttttcccc gggttccgcg agatcggcga gcattacaag
     3061 atcgacatcg ccctgttgcc gatcggggcc tacgatccgc cgactggccg cgaggttcac
     3121 atgaatccag aggaagcggt gcgcgcattt cttgagctcg aggcacaaac tttcgtcccg
     3181 atgcattacg gcacctttcg cctcggctat gagccgttgc atgaacccct cgagcgcctg
     3241 ctcgccgccg cccgcaccca cggtattgaa gaaaaaattg tggtaatgac ggaaggcaga
     3301 ccggtggtgt tgtgaaacag aaaacgctga acgctcaaca tcgaacgcca aatatcgaat
     3361 tcagaagcgc tgcacttcag ctcattggac gttcggcctc cgacgttcgg cgttttctgc
     3421 tttagatgtc acgatcgcgc cacacgcgat cagatattgt tggcgcggtc gccgccggga
     3481 cagccgtcct cgtcttctgt ctgcttctcc tctggcacga tccgctcgtc ttctggaacg
     3541 atgattacga attgtcggtc ttgcccgtct tcgcggatgt ggcgcggagc tggtcggaag
     3601 ggcactggcc gctgctcagc ccatactcct gggtctgcaa caatctcgcc ggcgagttcc
     3661 aatacggcac cttttccgtc ttcgtaaacg cggctgtcgt cctcatctgg aagtttccgc
     3721 tcagcttcgc gcagcaagcg gccgcgatct ccatcattca cctggttgtt ctggcgattg
     3781 gagcgctcct gctcgcgcgc ggccgagcct tatcgattcc gctgtcgatt ttcgttgcga
     3841 taatcgccgc actaaacggc tggatcattt gttggggcgc gactgattgg ttcggcgcgc
     3901 tcggcgcgtt cgcgtggctg ccgtgggcgt ggtgggcatg cgaacgcgcg ctcaaagggg
     3961 catcggcttc cagccgatgt tccgagaaca atcatcgaca agatgccgat gccacgcgat
     4021 cccgctggcg atttctttgg ccggcgccct ttgtgtatct cgtgattacc ggcgggtttc
     4081 cctacaccgt gttgatgctc gcgctggtgg tcgcgtggct ctcaatcaag tcgttaggcg
     4141 aaacgaaaca gtggatgtcg gttttgccga tgctcgttgg agtcgcgctc ggggccggac
     4201 tcgccgcacc agcgtggctg gctttgctcg attacgtgca cggttccgcc cgccagctgc
     4261 aaacagcgag cgctcactgg caatggattg tgccgtggcg cgcattgcca gggctcgttc
     4321 tgccagcgtg gacggtgaac tggcctgact tctccacgcg ctaccttccg cacgccgcaa
     4381 cggaactggc gtgcgggctc gttgcaccgt ccgcgctgat cgctggcttc atttggcggc
     4441 caaagttgct ggttaggcat ttgaaatggg aactcctcct gctcctgctc gtgctcctgc
     4501 tcgccatgct tcccacggct ggcgtgttcc gctggagctt tcgctggctc ccgctctttc
     4561 atctcgtgct cgcgatttgc ggcgccgagg tgttacgcct gcggccgggc tcaccgactg
     4621 gatctgcctc tgtcttattg ctgatcctcg tcacaatcgc cgtgttggtt ttcaacaccg
     4681 ccggcaagta cggccttgct gtgatgttcg tctactatga gatcgccgtg atctgggtag
     4741 ttggtgaatg gtttctgagg gatcggacgt ttcacgagtg ggctcctgcc gtcgtcacgt
     4801 tcagcatatt gctggcaacc tacatttgca ttccgcccaa ctgcggtgtt ccaaaatata
     4861 acttctcgca aaaactgctc tcgccctcgc cgctcgatcc gcagcggctc tatttgagca
     4921 tctatcctcc gaccgaacac acgtaccgcg tcgagaagaa accgcagccg gtcggccaaa
     4981 ttgtgcgacc cggcagcacg tcaatgtggg ccaccttgcg tttcgttaat ggctacagcc
     5041 caatcctcgc tgcgggtgtg gctcgtgaat tcaacttcgc gatccatggc gaaatcgatc
     5101 cggcgatggg cgattacttg ctcagtgacc ggtccgggct attgcagcaa ctcggcatcg
     5161 acggcctcat catcgcgcag gagatcgcga ctaatcccgc ctcaccagat tggcagcttg
     5221 tgttctcaaa caacgaagga cgcgtgtttc attggcgtgg cgggccgtta ccgcgcgtcc
     5281 gctcggtaaa tgagattgac tcgcattccg atgaaagatt tgtcacggcg gcgatctcgc
     5341 gaatcgacga ttcgcgaaat cgcgtggagc tcgatgtcga tgttccgagc ggaaaaaacg
     5401 ccgccctgct ggaattttcc cggccttatt ttcgcggcta cgtggcgcga ttaggcaata
     5461 tcaatctcgc ggtcgattcc tatcgcggcc ttatcccgct cgtcaaagtt ccacccggct
     5521 cgcatggacg gctcattctg acctatcggc cagcctggtt ggtttacggc ggtgcattgt
     5581 cgatcttgtg cgccgcaatt ttttccgtcg gcgttgtagc tgccgccgtc accggcggca
     5641 gaaccaaaaa taacgtcgag ttgccgcgag agacggcggc agctacaagt ctctagtaaa
     5701 cgtcgcgctt gtagcgtttc tcggtcttta gtttctcgac gtactgattg gcttcctcga
     5761 ccgatttgcc gccgtgctcc tggacgatct tccgcagcgc cgcatcgacg tctttcgcca
     5821 tgcgtttggc gtcaccgcag acatagaaat acgcgccgtc ccgatcgatc cagttccaga
     5881 gctcggctgc gttctcgagt attttgtgct gcacgtaaat cttgtgcgtt tgatcgcgcg
     5941 accacgcgca atcgaagcgc gtcaagatgc cgtcgcgcgt gaagcgttcc caatcttcct
     6001 tgtaggcgaa gtcgcatttc tcgcgttgcg cgccgaagaa gagccagttt tttcctttcg
     6061 caccctgaac ctggcgctcc tgcaaaaacg cgcggaacgg cgcgatcccg gtgcccgggc
     6121 cgatcatgat cagcggcagg ctccgatctt ccggcatatg aaaatgcttc gccaaactgg
     6181 gataaacggg caccggaacg tcgtccgcgc gctcggcgag aaaagatgaa caaactcctt
     6241 tgcgcagccg gccgcgggtt tcgtaacgca caacatcgac aatcaggtgc acctgatcgg
     6301 gataaacacg gagactcgac gcgactgagt agagccgcgg ctgtagttta gtgagcagcc
     6361 cgacaaattc cgccggcgtg aatcttgccg atggatgatc gagcaaaaaa tcgatcacct
     6421 cggcgcccca gaggtaattt tccagatcgt gtttgcgctc cgggtgtagc agttctttca
     6481 gcatcggtgc cgccgacgcg cgtcccgcaa tcgctttcag aaacttcggc gtcggctgag
     6541 tgatcgaata atcgcgcagc agggcttcac gaaacggttt cgtcatcttt gccgccggca
     6601 ccggttcatc gccggtcgcg cgcagcagcc gaataatttc attcacgagt tcgggatcgt
     6661 tggtcggata gatcgccacc gaatcgcccg gctcaaagct cagtccccag cccgtaagat
     6721 cgatctcaaa atggcgcgtg tccttttgcg attccggtgc gctcaaccgc cgattcacca
     6781 ggagcctggc ggggaaggga ttagcgcgcg tccacggcga agcaggtgca gctggtttgg
     6841 gcggagcgat gggcgcgagc attggaggat gtcgatttta cgctacggca attcacggag
     6901 caaacggcac gatttgcttt tgccagcgcg caagagtacg gacgcgcccg agcatgccgg
     6961 agcggacgcc taaccagttc gagccgccga gtgaacacgc gccgcctgtc gcggcagttc
     7021 caccgctccc gcgacatgat ccctatgccg catttcggtc gcgcggcttc agttttttca
     7081 cagcgggaaa tttgttatcg ctcaccggcc ggcaaatgct tgccgtcgcg gtggaatggg
     7141 aaatttaccg gcgcacccat tccgcgaccg cgctcggcct cgccgggctg gtgttcgcaa
     7201 ttccaatcgt cggcttgtcg ctgccggctg gtcacctcgc cgatcggatt agccgcaaac
     7261 agatcatcct ggtagcgcaa atcttcagcg cgtttacctc ggcaacgctc gcaattgttt
     7321 catggcaaca tctcgctatt ccgcagctgt cgatcttacg cgcgtgcagt cacggtctga
     7381 acgagatcgc gcgtgtcttt gagcggcaca acccacagtt ccacttcgat gatgcgtcaa
     7441 ttcccctcat ctacttactc ttgttcatcg gcgcggtcgc gcgcacgttt agttgggcgg
     7501 cgcgaagttc gttttttcca acgctcgtgc cccgcgacgc attctcaaat gccgtgacct
     7561 ggaacaacag tatcttccag attgcttctg tcgttgggcc aactatcagc ggtttccttg
     7621 tcgcacatat cggttttccc atcgtctacc tgatcgacgc cgtgaccgcg tttgcgtttt
     7681 tcctgctcgt tctgccgatc ccgcgtgcaa aacaggcgcg agatcgcacg ggacaaagga
     7741 cgtggcgcag tttgctcgct gcgatgaaat tcgtgttcaa caagaaggtc attctcgcga
     7801 cgattacgct cgacctgttc gccgtgctgc ttggcggcgc gaccgcgttg ctgccgattt
     7861 ttgcggacca gatcctgcgc tgcggcccgg tcggtcttgg ctggatgcgc gcggcgcccg
     7921 ccgtcggcgc gttcgccatg gcaatggtcg tggcttatct gccaccgatg cgacaggccg
     7981 ggcgaacatt gctctggtgc gtgacagggt tcggcgtcgc gacgactgca ttcggattgt
     8041 cgaaggcgct ttggttctcg ttgtcgatgt tgtttgtcgc gggtgcgctc gatagcgtca
     8101 gtgtcatcgt gcggggctcg atcgtgcaac tcgttacgcc cgatcaaatg cgcggacgcg
     8161 tctcggccgt gaacaatatt ttcatcggca cttcgaacga attcggcgcg ctcgaatccg
     8221 gtctgaccgc cgcgctcttt gggcccgtga tttccgtggt agccggcggc atcggcacaa
     8281 ttctcgtcgt ggttgcgtcg gcattgcgct ggcccgagat ccgcaaaatt ggccggctcg
     8341 acaaaacgtt gcgctaagat cgagaatgag ccgatgaaaa tcgccgctgc ccagatctcg
     8401 tgcgcgctag gcgatctcga gacaaatctc cgcaaggtcc gcgagtttgc ggcgcgcgcg
     8461 caagg
//
LOCUS       DASTDG010000016         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_618459, whole genome shotgun sequence.
ACCESSION   DASTDG010000016 DASTDG010000000
VERSION     DASTDG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618459"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFO30_00525"
     CDS             complement(<1..392)
                     /locus_tag="VFO30_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9417799.1"
                     /translation="MYRKILVALENSRADETLLPHIAELAKLHRAGLLLVHVADGFVA
                     RNYEQLNLAESQEMKEDRAYLETAAQDLRARGLEVDTFLALGDPAEGIVKAAEDRQCD
                     LIAMTAHGHRLLGDLLFGSTINEVRHKA"
     gene            complement(395..1756)
                     /locus_tag="VFO30_00530"
     CDS             complement(395..1756)
                     /locus_tag="VFO30_00530"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9417800.1"
                     /translation="MHEPATTRNGWRLARSTPSLPESHRTILIAPGAGFWRKLLAFSG
                     PGYLVAVGYMDPGNWATDLAGGSAFGYTLLSIILLSNLMAILLQALCARLGIATQRDL
                     AQACHDHFSAPVSFALWILCELAISACDLAEVIGSAIALNLLFHIPLVIGVCITALDV
                     LAVLYLQNKGFRYIEALVVALILTIGGCFLLEIIFSKPDLAAVFAGFVPNPEIIRNRD
                     MLYIAIGILGATVMPHNLYLHSSIVQTRNYEQTASGKAEAIKFATIDSSAALMFALFI
                     NAAILVVSAATFHKYGRHDIAEIQDAYKLLSPMLGVSIASVLFAFALLASGQNSTLTG
                     TLAGQIVMEGFLNIRLRPWLRRLLTRLVAIVPAVITAAMFGPSGTAKLLLLSQVILSL
                     QLSFAVIPLVAFTSDRRKMGEFVSPPWLQTLAWLTAAVIVLLNAKYLADWSGFSAWIA
                     RVW"
     gene            complement(1873..3276)
                     /locus_tag="VFO30_00535"
     CDS             complement(1873..3276)
                     /locus_tag="VFO30_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9417801.1"
                     /translation="MNSLLIQILPLADAPMTQWDSPGVVLAKLGAVLLLVCLNGFFVT
                     SEFALVKIRTSQLDALIAEGNKRAGIGRHVAGNLDAYLSACQLGITLASLGLGWLGEP
                     FVAELLQPFFALVNITSTAVITSISFGLAFSIITFLHIVLGEQAPKILAIRKALPATL
                     WVSPPLRLFYTLFKPAIWFLNASSNWVLRYVLRIQPAAAHELAHSEEELRFIFEESEK
                     SKEVSPLGRKLVVNVLDLRERVVRDIMTPRGEVVYLDLEDDFETNAQKALASKHTRLP
                     LCRENLDNTVGLIHIKELVPMMRDPNPDLMRIKRDLIPVPEMMPLEKLLNLFLSKHAH
                     LAIVVDEFGGTVGMVTLEDVLEELVGDIQDEFDTEKEEFRQINENEFVVDGALGLYEL
                     RDAAGLELDSADVSTIGGYVTHLLGHLPKQGEHVKIDNYDVTVSQADSRRVRQLHFRK
                     LADAPAEPATTRPDVAL"
     gene            complement(3341..3610)
                     /gene="rpsN"
                     /locus_tag="VFO30_00540"
     CDS             complement(3341..3610)
                     /gene="rpsN"
                     /locus_tag="VFO30_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET9417802.1"
                     /translation="MAKTSWINRNERKRATVKKYAALRAELKAKKDYAGLAQLPRDAS
                     PTRMVNRCEVTGRRRAFIRRFKVSRLTFRELASQGMIPGVTKSSW"
     gene            3741..>4259
                     /locus_tag="VFO30_00545"
     CDS             3741..>4259
                     /locus_tag="VFO30_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417803.1"
                     /translation="MFPLQRRSLPASKDELANAVEEGLRRFVHKTGPIVDVRSRVFPY
                     IDEIVINLDGAKIDALPPRSQGIAGGTKPAFEVAAVNLTARNIGVRGVPLNLQFEARG
                     VCLDQAFDARGEIVLLPRRIDDGEISFSAAQLDVEEAIARILATEARAQGITIEQVRL
                     ALRERGPRSIAGE"
BASE COUNT          926 a         1251 c         1245 g          837 t
ORIGIN      
        1 tgcgctttgt gccgcacttc gttgatcgta ctgccgaaga gtaaatcgcc taacaaacga
       61 tggccgtggg ctgtcatcgc gatcaaatcg cattgtcgat cttccgccgc tttcacaatg
      121 ccctcggccg ggtcaccgag cgcaaggaag gtgtccacct ccaggccgcg cgcgcgcaga
      181 tcttgggccg cggtttccaa atacgcgcgg tcctccttca tctcctgcga ctcggcgagg
      241 ttcagctgct cgtagttgcg cgccacgaat ccgtcggcca cgtgcacgag cagcaagccg
      301 gcacgatgga gcttcgccag ctcggctatg tgcggcagga gcgtttcgtc cgcccgacta
      361 ttttccagtg cgacgaggat tttccgatac atcgctacca cacgcgcgcg atccacgcgc
      421 tgaatcctga ccaatcggcc aggtattttg cgttcaacaa cacaatcact gcggcggtga
      481 gccaggcgag cgtttgcagc catggcggag acacgaactc gcccattttg cgacgatcgc
      541 tcgtaaacgc gaccagcgga atcactgcga acgagagctg caggcttaag atcacctgac
      601 tgagcaaaag caacttggct gtgccgctcg ggccgaacat cgccgccgtg atgaccgccg
      661 gcacgatcgc gaccagtcgg gtgagcaagc ggcgcagcca cggccgcaag cggatgttga
      721 gaaatccttc catgacgatc tggcctgcca gcgtgccggt gagtgtcgaa ttctggcccg
      781 acgcgagcag cgcaaacgcg aacagcacgc tcgcgattga cacgccgagc atcggcgaaa
      841 gaagtttgta ggcgtcctga atttccgcga tgtcatgtct cccgtatttg tggaacgtcg
      901 ccgcggaaac gacgagaatg gcggcattaa taaacagcgc aaacatcagc gccgcgctgg
      961 agtcaatcgt cgcgaacttg atcgcctccg ccttgcctga cgcggtctgc tcgtaatttc
     1021 gggtctgcac aattgaagag tgcagataga gattgtgcgg catcaccgtc gcgccgagga
     1081 tgccgatcgc gatgtaaagc atgtcgcgat tgcggatgat ttccggattc ggcacaaatc
     1141 cggcaaacac ggcggccaaa tccggtttcg agaaaatgat ttcgagcaag aaacagccgc
     1201 cgatcgtcag aatgagcgcg acgaccagcg cctcgatgta acgaaagcct ttgttctgca
     1261 aataaagcac ggcgaggacg tccagcgccg tgatacaaac gccgatcacg agcggaatat
     1321 gaaacaataa gttcaacgcg atcgctgagc cgatgacttc cgcgaggtcg caggccgaga
     1381 tcgccagctc gcacaagatc cacaatgcga acgagactgg cgcggagaaa tgatcgtggc
     1441 acgcctgcgc gagatctcgt tgagtggcga tgccgagccg cgcgcagagc gcctgcagca
     1501 ggatcgccat caggttcgag agcaagatga tgcttagcag cgtgtaacca aaggccgagc
     1561 cgccagccag atcagtcgcc caattgcccg gatccatata acccaccgcg accaaatatc
     1621 ccggacctga gaaggcgagc agtttgcgcc agaaccccgc tcctggcgcg atcaaaatcg
     1681 tgcggtgcga ctccggcagg ctcggcgtgc tgcgcgccag tcgccacccg tttcgtgtcg
     1741 tcgcaggctc atgcatcggg cacaaagcta ttgtcgatct tgcgcttggc gcaaacacga
     1801 tcgcgtcctg tagccaccgg ccatggcccg tctgccgacc gtctgatcgc gcacagtgcc
     1861 gtcgctacag aatcacagcg ccacgtccgg tctggtcgtg gccggttcgg cgggtgcgtc
     1921 ggcaagcttg cgaaaatgca attggcgaac gcggcgactg tccgcctgcg aaacggtaac
     1981 gtcgtaattg tcgatcttca cgtgctcgcc ctgttttggc agatggccta acaagtgagt
     2041 gacgtaaccg ccgatcgtgc tgacatcggc gctatccagc tccagaccgg ccgcgtcgcg
     2101 caattcgtaa agcccgagcg cgccgtcgac cacaaactcg ttttcgttga tctgccggaa
     2161 ctcttccttt tcggtgtcga actcatcctg gatgtcgccg accaattcct cgagcacgtc
     2221 ttcgagcgtg accatgccaa ccgtgccgcc aaattcatcg accacgatgg cgaggtgcgc
     2281 gtgtttgctc aaaaaaagat taagcaattt ctccaacggc atcatttccg gaaccggaat
     2341 caagtcgcgc ttgattcgca tcaagtcggg attcggatcg cgcatcatcg gcacgagctc
     2401 cttgatgtga atgaggccga cggtgttatc gaggttctcg cggcaaagcg gcaagcgcgt
     2461 gtgcttcgac gcgagcgcct tctgcgcatt cgtctcgaag tcgtcttcca aatcgagata
     2521 aaccacttcg ccgcgcgggg tcatgatatc gcgcaccacg cgctcgcgca ggtcgagcac
     2581 attgaccacg agcttccggc cgagcggcga aacttccttc gatttttcgc tctcttcgaa
     2641 aatgaagcgc aattcctctt cgctgtgcgc caactcatgc gccgcggccg gttgaattcg
     2701 caaaacatat cgcagcaccc agttggatga agcattcaag aaccagatcg ccggtttgaa
     2761 caaagtgtaa aatagccgca acggcggact cacccacaaa gtcgccggca gcgccttgcg
     2821 gattgccaga atttttgggg cctgttctcc caagacaata tgcagaaagg tgatgattga
     2881 gaacgcgagt ccgaaagaga tcgaagtaat gactgcggtg gaagtgatat tgacgagcgc
     2941 gaaaaaaggc tgaagcaact cggccacgaa cggttcgccg agccatccca ggccgaggct
     3001 cgcgagggta atgccgagtt ggcaggcgga aagataggca tctaggttgc cggcgacatg
     3061 gcggccaatt cccgcgcgtt tgttaccctc cgcaatcaga gcatcaagct ggctcgtccg
     3121 gattttcacg agcgcgaatt ccgacgtgac gaaaaatccg ttaaggcaaa caagcaaaag
     3181 aacggcgccg agcttcgcca gaaccacacc cggcgaatcc cactgcgtca tcggcgcgtc
     3241 cgcaagcgga agaatttgga ttaacaaaga gttcattttt tgtagccacc ggcctgtggc
     3301 cggttttggg tgggacgtcg catagcgacg tggctacatt ctaccagctc gacttcgtca
     3361 cgccgggaat cattccctgt gacgccagct cgcggaatgt cagacgcgag actttgaacc
     3421 gccgaatgaa cgcgcggcgg cgcccagtca cctcgcagcg gttgaccatg cgagtggggc
     3481 tggcgtcgcg cggcagttgc gcgagccccg cgtaatcctt tttcgctttt aattcggccc
     3541 gcaacgcggc gtattttttc accgtcgcgc gtttgcgctc gttgcgattg atccaggagg
     3601 tcttggccat aaatgaagcg cagaatgtag tggaggcgga cgcgttttca actcgctttc
     3661 ataaggtcga ccggcaagcg gagcgcttgg cgtacaattc tgccgttgaa cgtcaagcct
     3721 ctgcgtgaaa cgtctcgcca atgttcccat tgcagcgccg gagtcttcca gcttcgaagg
     3781 acgaattggc taatgccgtc gaggagggac tccgccgctt tgtccataaa accgggccga
     3841 ttgtcgatgt tcgctcgcgc gtgtttccat acatcgacga gatcgtgatc aacttggatg
     3901 gcgcaaaaat cgacgcgttg cctccgaggt cgcaaggtat tgcgggcggg acaaagcccg
     3961 cgttcgaagt tgcggcagtc aacttgacgg cgcgcaacat cggtgtgcgc ggcgtgccat
     4021 tgaatctgca attcgaagcg cgcggcgttt gtctcgatca agctttcgat gcacggggcg
     4081 agatcgtgtt gctaccgcgc agaattgacg acggcgagat ctcattttca gctgcgcaac
     4141 ttgatgtgga ggaggcgatc gccaggatcc ttgccacgga ggcgcgggcc cagggcatca
     4201 cgattgagca ggtgcgacta gcgctgcgcg agcgcggccc gcgctcgatc gcgggtgaa
//
LOCUS       DASTDG010000017        31292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_484175, whole genome shotgun sequence.
ACCESSION   DASTDG010000017 DASTDG010000000
VERSION     DASTDG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 31292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31292
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484175"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFO30_00550"
     CDS             <1..623
                     /locus_tag="VFO30_00550"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9417804.1"
                     /translation="KRMLFKFSGNSWLGIHLGMTGKIDVAPPQFQPAQHDHLVLYQRK
                     RTLVFTDSRQFGRIRFHHGTDEPDWWKSDAPEIMAREFDRQFVDEFLDRHRKAPIKAA
                     LLLQNGFPGIGNWMADEILWRANILPSRRVGSLSNGDRARLRRAIKFVVRRSLETLGK
                     DFSDPPRKWLIHQRWSRTGICPRHRTPLRYATIGGRTTAWCPRCQK"
     gene            complement(633..2189)
                     /locus_tag="VFO30_00555"
     CDS             complement(633..2189)
                     /locus_tag="VFO30_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9417805.1"
                     /translation="MSRSKALFAGLLAGLVAGIAMTVVMLLLACFGVATPLAIIGDRL
                     SVFIPPGPFLALMGKVGGYNHLKQLGVGSTIAGQVLVAATAGAIFGLFMRRDPARRVT
                     LITIGVFVLLPIIAVTIALWPVLGTSYGGFPIPVARLVTLLGFALSVFVFERILVATF
                     RFLTQRKIDDEAAEFSPAIGRRALILGGVGLVLAGGGAALLRRLYRAATFSYDGTQYK
                     GRIVQPITPNELFYCVTKNVIDPRVDVDVWHLEVNGLVQNPATYRFRDLKGFAATEQE
                     TTLMCISNGLDAGLISNAVWTGVPLHDLIDPAAPLANAARVRLHGVDNYTDTIPLEKA
                     LEPTTLLAYEMNGKELPHRHGYPLRAIVPGYFGEKHVKWITRIELTTNDAKGFYEAQG
                     WGPDFITPTRSRIDIPDHESRFSIRELRRPIEIKGIAYGGDRGISRVELSFDDGETWD
                     DADIYYAGGDLAWSLWAAHEGWSPNEPGDYTLVVRATDGEGVVQEWEPDRSPFSGATG
                     FHKIVVHVIA"
     gene            complement(2320..3012)
                     /locus_tag="VFO30_00560"
     CDS             complement(2320..3012)
                     /locus_tag="VFO30_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9417806.1"
                     /translation="MSVSVVIPALNEEEPIAGVVRECLATGVPNEILVADNGSTDRTA
                     ERAREAGARVVSEPTRGYGRACTAGVRAVSRKNEVVVFLDGDGSDCPEFIPQLVEPIA
                     RGTHDFVIGSRTRGKREPGSMNLQQIFAGRLAGWLLSILYGVRYTDMSPFRAIRRDAL
                     EKLGMREKTYGWNLEMQMRVARAGLRILEIPVNHRCRAGGISKVSGTLRGTFVAGARI
                     IATLVRIALERS"
     gene            complement(3009..4025)
                     /locus_tag="VFO30_00565"
     CDS             complement(3009..4025)
                     /locus_tag="VFO30_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9417807.1"
                     /translation="MPELAGIPDLESRVVEHARKDFPLLEADSTVADALERIRREGIG
                     ERVIYFFAVDADKRLVGVLPTRRLLTAPLETPLRDIMVRRVVAIPASATVLDACEFFV
                     LYKFFAFPVIDEQRRVVGVIDVSLFAEEMLQAGESEDRHRPAAPVHDDIFEALGFHLE
                     QIRGASPWRVFRYRFPWLLATVASGTICALLAGAFEATLARSLVIAFFLTMVLGLNES
                     VSAQSMSVTIQTLRSASVTWRWFVSALRRELTTAALLGLGCGIVVAMIVWLWRNDARG
                     ACVIGGSISLSLVSACLFGLGVPSLLHRLKLDPKIAAGPITLALADFFALVIYFTSAW
                     LVLE"
     gene            complement(4018..5148)
                     /locus_tag="VFO30_00570"
     CDS             complement(4018..5148)
                     /locus_tag="VFO30_00570"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9417808.1"
                     /translation="MTKRALVTGGAGLIGSHIVDLLVRESWAVRVLDNLEPQTHKRGK
                     PPWIDERVEFVHGDMRDRDTLAAALDSVDIIFHQAAYGGYMPEIAKYVHVNSLGTAQM
                     LELIREKNLPIKKIVAASSQAVYSEGAGECRKHGLVFPPVRPVAQLRKGDWQVHCPIC
                     GAVTTSVPTPEHAPIGGETVYGLTKVDQEKLVLLWGKQVGIPTVGLRYSCTYGPRQSM
                     FNPYTGVIAIFCTRLLNNLPPVLYEDGEQTRDFSFVEDIARANLLAAESDKLDGFAVN
                     VGSGRGVPIREIAETISAALKIDIEPVARGEFRPGEMRHLTSDITRIRSIGYEAQVEL
                     ADGIQRYIDWIKAQTDVRDYFSEAEQILKSKGIVHRVEVMNA"
     gene            complement(5174..5866)
                     /locus_tag="VFO30_00575"
     CDS             complement(5174..5866)
                     /locus_tag="VFO30_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET9417809.1"
                     /translation="MTKAPRAGQVKTRLVPPLTPDEAAQLNSCFLRDTTAAISAAAGS
                     VACGIAVYTPVGAESDYDDMLPPDFALLPQRGDHFGARLINAAEDLFRIGFSSVCLID
                     SDSPMVPVEVYAEAPKLLSTDEPRILLGPSDDGGYYLIGLQRLCRRVFENIDWSTERV
                     LQQTIARARELQVRVELLPIGYDVDDGATLRRLCQELLDTRATSTNDVAPATREFLSE
                     IVAREGRSRICA"
     gene            complement(5964..6674)
                     /locus_tag="VFO30_00580"
     CDS             complement(5964..6674)
                     /locus_tag="VFO30_00580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9417810.1"
                     /translation="MPPQIIVATEKPLRSRSPAAARPSDSLFEHVAWVYAFCRERLFR
                     DDTERIVATLWPNGGAQPGTQVIELGCGPGFYSANLAKRFPNISVTGVDRSANQLKWA
                     RERAETESLRNCAFVRVNVLTLPFTDAQFDVLLASRLFTVLPDCARAVAEMHRVLKSG
                     GRCFIAEPRYALWASIPLTAMWLLARMTHSGNGYREPRKATVFSPTTFEKLFSSQPWR
                     RVKTWHDGRYQYALCEKR"
     gene            6944..7774
                     /locus_tag="VFO30_00585"
     CDS             6944..7774
                     /locus_tag="VFO30_00585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9417811.1"
                     /translation="MQTRTTSLFCATALFTVVVSSAGAAELPPLWKIQQTLAAKRYVD
                     LTHAFTPGIPRWPGFPDETRKTIYWYDKRPDTMGAGFFSELFTHVGQWGTHVDPPAHF
                     IKGLRTVDQIDPKEMILPLVVVDVHRECEQNPDYTLTVDRLKKWETEHSQIPAAAFVA
                     MRTDWSKRWPDAAKMENKDAKGVAHYPGWSLPALKYLYEERKITASGHETTDTDPGVA
                     TTKEDYSLETYILSTNHYQIELLTNLDQVPEAGALVVVSFPKPKDGSGFPARVFAILP
                     "
     gene            complement(7829..8959)
                     /locus_tag="VFO30_00590"
     CDS             complement(7829..8959)
                     /locus_tag="VFO30_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9417812.1"
                     /translation="MSVLHQTTAARAFRKTAETFAPGAKTLPQRYFVSPEVFAIEQEK
                     IFSRHWLLVGHQSQLPQAGDYFVVNIASESLIITRDQRSTIRGFYNVCRHRGTRLCEK
                     AKGHLSAIQCPYHAWTYRLDGRLIGAPHMDEVPGFNKADYSLNPVKLAVWESFIFVNL
                     GKTPVSLEEWFAPLAGKFSHWNMSILRPARHIEYDVRANWKLMFENYSECYHCPGVHP
                     MLSKISPYDSAENDLAEGPFLGGFMSINKGQSLTMSGNACASFVGDIKNLQRVFYYSI
                     FPNMLLSLHPEYVMVHQLWPQAPDHTRIVCDWFFHRDAFDRRDFNPEDAIAFWDMTNK
                     QDWHVCELSQQGIASRAYQPGPYSARESIPAAWDREYLRQVR"
     gene            9107..10048
                     /gene="metF"
                     /locus_tag="VFO30_00595"
     CDS             9107..10048
                     /gene="metF"
                     /locus_tag="VFO30_00595"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET9417813.1"
                     /translation="MRRDSPTFSFEFFPPKTPEAAETLYQTIRDLESYMPHFVSVTYG
                     AGGSTRDLTHHLVERIQHTTKLDPIPHLTCVCHKEEEIDAILARYAKSAIGNILALGG
                     DRPRDIPYDKSRDSFQHAVDLVRFIRRFNESGAHPDRRGFGIGVAGFPEGHPATPNRL
                     VEMDHFKAKVDAGADYVVTQLFFDNRDFYDFRERCALAGVHIPIIAGIMPITSMSGMK
                     RIAELAGGARFPARLLRAVQRCDNDPEMVRRVGVHFALEQCHDLLDNNVAGIHFYTLN
                     RSDATRVIFDSLGIPRRRREGAPTALSSDDIHQRLAK"
     gene            10136..10885
                     /locus_tag="VFO30_00600"
     CDS             10136..10885
                     /locus_tag="VFO30_00600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9417814.1"
                     /translation="MLNTRRAVYVQVGGVALALALLLLLSRFLPIIDLIERSENAVMH
                     WGTWSMVGYPLLFALCNLLLLPGGLLSVGGGFFFGLWPGFLLVLLGNTIAAATSFALS
                     RWVGRRWFSQRFVQSRVLQLLEPAVEREGWKIVALSQLHPMFPTSLLNYFYGLTRIRF
                     AACMLWTCVGRVPGLFLYVYLGTLGKVGFNVARGVHHPHIAEYWTWVAALGVAVLLVI
                     LLTRMAVREMKMAEEVETAGNESDPQQVINA"
     gene            10980..12881
                     /gene="mnmG"
                     /locus_tag="VFO30_00605"
     CDS             10980..12881
                     /gene="mnmG"
                     /locus_tag="VFO30_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET9417815.1"
                     /translation="MFKLPTEYDVVVVGSGHAGIEAALAAARLGCRTLMLTQNLDTVG
                     QMSCNPAIGGLAKGHIVREIDAMGGAMGINADATGIQFRMLNRSKGPSVRAPRAQCDK
                     KAYQFRIKAVLERAANLDLKQATVSRVIARDGCVTAVETDIGVMIQTRAAVITSGTFL
                     RGLLHVGQTSKPGGRMADAASGLSDNLRQLGFQVGRFKTGTPCRLHRRSIDFSKCQIQ
                     LGDEPPPRFAFLPEVGAGRNDIFTLNGEHAGVFHVEQLPCWVTQTTPRTHEIIRANLH
                     RSPLYAGRIKGTGPRYCPSIEDKVVKFAERSSHHIFLEPEGRHTEEFYVNGISTSLPY
                     DVQIEFVHSIPGLERAEIMRPGYAVEYDYFPPTQLLHTLETKLIVGLYFAGQVNGTSG
                     YEEAAAQGLIAGANAALKVQGRQPLILQRNTSYIAVLIDDLVTKGTEEPYRMFTSRSE
                     DRLQLRQDNADQRLTPLASRYGLIDEARSSVLGSKMQLLQDARRIAAEAKVQGAPILQ
                     LLKRPGFTFSYLPAEIARAVPAEIWELLETDLKYEGYAARQRDHNRQLTCREAQPIPD
                     AVDFRAIRGLSLETRQKLSAIRPTSLGQAARISGVTPADISILSIWLSSKGLQLSNQP
                     RSTIYSPDT"
     gene            12902..13528
                     /gene="plsY"
                     /locus_tag="VFO30_00610"
     CDS             12902..13528
                     /gene="plsY"
                     /locus_tag="VFO30_00610"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET9417816.1"
                     /translation="MSAYATVAVLGYLIGSFPAGYVAGRIAGVDIRTHGSGNIGATNV
                     LRVLGKRFGYAVFLVDFGKGVAAVLVARHITSVNGTDERAVELAATLAGIVAVIGHSF
                     PVWLKFRGGKGVATSIGVLFALLPLAGAVVCLVWLATFEIARYVSLASVVAAVALPIA
                     VAAMFFLHRLQTPVLLYCSLCLAALVIVRHRSNLARLAKGTEPRFSRK"
     gene            13525..14526
                     /locus_tag="VFO30_00615"
     CDS             13525..14526
                     /locus_tag="VFO30_00615"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9417817.1"
                     /translation="MNLQKLAIIGAGGWGTALAVLWAKLAPEVVLWGNSAARVEKMRA
                     TRENQVYLPGICLPDSLGLTSRLDKCSDADLIVFVVPSTALRAIADRLRDIAGSNHAV
                     LLTCTKGVEHGSGMRMSEILTQIFPERAIAALSGPNLAAEVARGLPTATVLACRQPEY
                     ARELQGLLGSPRFRIYTGDEVPGVELGGALKNVFAIAAGASEGLGLGDNSKAALVTRA
                     LAEMIRLGTAMGGQLRTFYGLSGAGDLVLTCYSKQSRNRSVGERLGRGESLRQITSTT
                     QMVAEGIPTAKSAYGCARKLKIDTPIIDQVYALLYENKPAVQALEDLLGREQKPERV"
     gene            complement(14523..15113)
                     /gene="purN"
                     /locus_tag="VFO30_00620"
     CDS             complement(14523..15113)
                     /gene="purN"
                     /locus_tag="VFO30_00620"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET9417818.1"
                     /translation="MRPLGLGILGSGKGSNCRVILESICSGVLPAEARVVISDVLDAP
                     ILEIAREFTVPNAYLPPGQFRTRLEPRVEVELVQMLRDAGVELVVLAGFMRVLKQPML
                     QAFPRRIINIHPSLLPKFPGLEAWKQALAAGETVTGCTVHYVDEQVDHGDIIAQRQVA
                     ILPNDTEESLHARIQIAEHKLYPAVIAQFCAAESRA"
     gene            complement(15110..15598)
                     /gene="rfaE2"
                     /locus_tag="VFO30_00625"
     CDS             complement(15110..15598)
                     /gene="rfaE2"
                     /locus_tag="VFO30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET9417819.1"
                     /translation="MKHSKIIAFDEISERAKQLHEAGKKVVATNGCFDLLHVGHVRYL
                     QRARSLGDLLVVGLNADTSVRALKGEGRPINREHDRAEVLAALESVDLVTIFPEPRAT
                     RFIQAVRPDVYVKGGDYTSDSLDAEESALLEKLGAKIDIVPFEHGYSTSQLLNKLRTA
                     AE"
     gene            complement(15595..16536)
                     /locus_tag="VFO30_00630"
     CDS             complement(15595..16536)
                     /locus_tag="VFO30_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9417820.1"
                     /translation="MLRDQSRKIDNRSARSRKSAKPWVIFNPNAGSIGDIDAVIAQLN
                     RLDPAVIRMTRKARDAQRFAREALRNNYEWIITAGGDGTLNEVVNGVASCAGRVCLGL
                     MPLGTGNDFARSLNLPTAVEDNIDILLSKKTKPIDIVRVRSSRPSRYFVNVSAGGFSE
                     VVDEQLTPDMKQTWGPLAYLRSAAAALPKLHVYHAHIEFDSSEVVRVDLYNIVVANGR
                     FVAGGLPIAPNADPGDGLLDIVLIPTHPAAKVALLAAEILLGKHFKSNAVEFRRAKKI
                     AVRSRPAMWFNVDGEPVGTVPAVFEVVPRALNFVVPE"
     gene            16583..17362
                     /locus_tag="VFO30_00635"
     CDS             16583..17362
                     /locus_tag="VFO30_00635"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET9417821.1"
                     /translation="MPPPNSKSPAEPDRRDFRVSRGSLVTLAETERVDFIPNAVELPR
                     FYGEPLLFAIGRDPRTIFAYWSIDWASIFDTTAPVDRQVHLRVYRDDGAEETAAAAEP
                     MAGNCYITVSQPRARYRVELGYYQPKNSWHGIATSEEVTMPPDSVSEATDVDLATIPL
                     HLSFQRLIDLFRANNSDALTEIISRLQRRAVTAAERDLLTPEELEILRAMNVSLEEME
                     SGRALFHDRQMSEAALRRRAETLLGFGASSRGHAFSASSWG"
     gene            complement(17370..18878)
                     /locus_tag="VFO30_00640"
     CDS             complement(17370..18878)
                     /locus_tag="VFO30_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9417822.1"
                     /translation="MASLFAKKPLDRLIEEAHEVGEHTLKRSLGPVNLVMLGIGAIIG
                     AGLFVRTAAAIADRAGPSVVLAFIVAGVGCTFAGLCYAEFASMIPVAGSAYTYSYATM
                     GEFVAWIIGWDLILEYAVGAATVAIAWSEYANRVLGWFGLRIPYQWSHSPFEHDIGSG
                     IHGIMNIPAVFILLLLTLLLIRGTRESAFVNGLIVILKVSIVLLVIGIGWRFMNPANH
                     TPLIPAPTTYVTPQGIQHAYGGILGILGAAGVVFFAYIGFDAVSTAAQEARNPSRDMP
                     IGILGSLAICTVLYVLFSYVLSGVASVQDFRTAGREASVAFAITKYMPGFEWLSKSVT
                     VAILAGFSSVILVMLLGQSRVFFSMSRDGLVPKTFSQVHPRFRTPYKSNMLFFVFTGL
                     FAAFIPEDVVGEMTSIGTLFAFILVCAGVWIMRVRRPDLERAFRVPAAPVVAILGIIV
                     CGAMIYGLGWTNWMRLIVWLLIGLVFYFTYGKQHSRITAPPPELPEGMKLTR"
     gene            complement(18929..20476)
                     /locus_tag="VFO30_00645"
     CDS             complement(18929..20476)
                     /locus_tag="VFO30_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9417823.1"
                     /translation="MNSGTAIADTPVAEQKFVRGLGLLDSTMLVAGSMIGSGIFIVSA
                     DIARLVGSAGWLLVVWAVTGALTITAALSYGELAAMMPRAGGQYVYLREAYTPLWGFL
                     YGWTLFLVIQTGTIAAVAVAFARFLGVFAASISPTNWIVPPIILSRNYAISLSTQQLI
                     AILIIVCLTIVNTRGLSLGKLIQNIFTSAKTLSLFALIVLGIFVGRNADALQTNFTNF
                     WTPGIVSPIKSDLSFIAAVSATGGALGMLIAICVGQVGSLFSADAWNNITFTAGEVRE
                     PRRNIPLSLALGTGLVITLYLLANVAYLCVLPLDKIQHAPDDRVGTAAMQVMFGGAGA
                     AIMAVAIMISTFGCCNGLILAGARVYYAMAQDGLFFRSTGRLNKRHVPAVALVLQGVW
                     TALLVLPRTRLRNAAGEVQYGNLYGTLLDYVVFAVLIFYVLTIAGVFVLRRTRPEAER
                     PYRAFGYPLLPVFYIVAAMIILIVLAVYRTQTSWPGLLIVLAGVPVYFLWRKSKRDDS
                     SERERCA"
     gene            20653..21933
                     /locus_tag="VFO30_00650"
     CDS             20653..21933
                     /locus_tag="VFO30_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417824.1"
                     /translation="MFTLAAYAVPVVAVVGVPLEFVLFGLTLLSLAIFHHRTMRVAVA
                     GLVIISVYKVAFTGFKTGPGLAGLWVHLAQEWVILVNLLCLLTAFALLSRHFEKSHVP
                     VVLPRFLPHDWKGGFLLLLMVWVLSSFLDNIAAALIGGAMAHQLFRAKVHTAFLAAIV
                     AASNAGGAWSVVGDTTTTMMWVAGVSPAAVFRAIIAAIVALLVFAVPAAKRQHNYSPI
                     LQHAHEHTRVDWIRVGIVSSMLAFAIVTNVAISLFLPKSADRFPFIGVAVWLALLISV
                     PLRRPDWEFLPRTIRGSIFLLALVLCASMMPVEELPAPSWQTTFGLGLLSAAFNNIPL
                     TALALQQGGYDWGFLAYAVGFGGSIIWFGSSAGVAVCTLFPQARSVTNWLRDGWWVIA
                     AYVIAFFAMFTVWKWHPDKPRPAKSVPAATLLMK"
     gene            21930..23333
                     /locus_tag="VFO30_00655"
     CDS             21930..23333
                     /locus_tag="VFO30_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417825.1"
                     /translation="MTPRAARTLIVLSIFVIEALLVYFLVARRFPFSGDDYSYLYQAR
                     LFASGRIYAEDPFYDPKLPFYDCIQTYCLRDNRGHRFSKYPPGWPALLGLGARVRAAW
                     LVDPILGALLLLLVINHAERRWGEHFATNTSLLLLLCFFFVYYAASFRAHIATALFIF
                     AAFLVYDANERRAARSKLWLFLSGALLGYSAMIRYIDWIPLALWNGSHLVRRNRFVDL
                     GVFALGFSILASGNLLYDALLFGDPLQAPATMYHFSAGINDHLFLSWRSFTVTAARLG
                     RLLLVFPPLLLLITALRNVQVWAEAKTYLGLFLLSVATYFFYPEAVGGPGPRYFLGYF
                     PFLVLAVVAVYRRMVEQATRLARPLWNAALLTLVLCNAVFIAKASYVMHGRRDLERTA
                     EQLSDRNKKIIFLKTGTYDTPVGDLTRNPPVLSSARTLYFKWCDQSDVAALLQRFAGR
                     SVYVYEYPGRLQRYNRR"
     gene            23462..24442
                     /locus_tag="VFO30_00660"
     CDS             23462..24442
                     /locus_tag="VFO30_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET9417826.1"
                     /translation="MNTRRGRSSATCGLSILLFMAFCAGETFAAPRSRRTPSQAPPKF
                     EALPLVRSRQGHLIVEAFINGKPALLGVDSGAPLSAIAENRRQYFGLTGVSGSRLPAR
                     LQINGAFNSVAIVRRFRLGALSLLDHPVVVIDLDGSSRSARVLHEQEIDGIVGADILF
                     PTQAVLDCQRQLLILKVDPDVTGSTPGIEYRGLRSVPIRVSEDYNLYVDGAINGAPAQ
                     LMVDTGAFATLLHRDFVRRMRIPLRETPFRGAAVNLKQRGVQIARIRRLSVGSVDIVG
                     KDVGVIDLSGLVRHGLLEASPPVAGLLGAEILRSHHGIIDFGTRTLYLKR"
     gene            complement(24528..25028)
                     /locus_tag="VFO30_00665"
     CDS             complement(24528..25028)
                     /locus_tag="VFO30_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9417827.1"
                     /translation="MPEETVWRGTSSQWKNFGRYILAVTMSVVVLVIFAALSRADSLQ
                     MRNLSPYILLLLALPLLLALVRYLQTKSKVYELTTERIKISEGILSKVTDTLELYRVK
                     DLETRQPFLYRVVGIENVQLNTSDTSSPFVFIQAVPRRVGLADKIRNQVEIIRQQKGV
                     REIDVE"
     gene            25046..25996
                     /locus_tag="VFO30_00670"
     CDS             25046..25996
                     /locus_tag="VFO30_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9417828.1"
                     /translation="MTAITQAFVLGAGLGTRLRPLTDDLPKPLVPVFQKPLITFALDH
                     LIDTGVNSFVINTHRLPELFRDFFRENNYTGRSVTLVHEPHLLDTGGGMKNIERFLGT
                     RPFVVYSGDILTDADLEPLIEEHFRAKNDVTLGLRRNTGLGAGVVMRDGRISAISRES
                     NPKENFDYANISVWNPEIFHRIPAKRKISFIPILVDWIGAGGKIGGLELDDGKWFNIG
                     SRKEYLDVHRDISTERWKPRYVTTRDWSEPVAKSAQVDATAEIRGCSVVGADCRVEAD
                     VILEDTILWPGAQIASRSELHRCIVRTHRKVSGTHRNLDI"
     gene            26001..26987
                     /locus_tag="VFO30_00675"
     CDS             26001..26987
                     /locus_tag="VFO30_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9417829.1"
                     /translation="MRTDLLLRRTRMHFPRLDTDKVKIRPIEKGGSDRKFYRVRCSPE
                     QTLILVKYNLEREENRHYVAIAHFLAEHGIRAPKIYYHDAEEGLIWLEDLGERDLFSY
                     RHESWLVRRAFYESALDEIAKLHRLPESVCLAMHQHLPAEFNAALYRWEQNYFFENCL
                     GRFYKIDNVALAPLAALPALNEIAERLDKLPRVLVHRDFQSQNIIVRNSQTHLIDFQG
                     MRPGLAQYDLASLLYDPYVDLSNAEREELLDHYCGGKPSAEFREILRLCAIQRLMQAL
                     GAYGFLGLVKGHKHFLKHIPRAMRSVCEMISNTAGLEKLAAVVCELADERVR"
     gene            complement(26964..27575)
                     /locus_tag="VFO30_00680"
     CDS             complement(26964..27575)
                     /locus_tag="VFO30_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9417830.1"
                     /translation="MIYETFCGGIFETNCYLVEAPEGLMLFDAPDGACDWLERRNVDL
                     KLLLLTHGHVDHVQGVAKIKRRFNCPLGCSAETATMISDRDFFRNFGFELEIEPAEAD
                     FIISETPARDFLGATMQVLEVPGHCPGSLCFFSRNEELLIGGDVLFAGGVGRWDLPGG
                     DVDLLFAGIRTKLFPLGDNVTVLPGHGPPTKIGTERLTNPFVS"
     gene            27767..29986
                     /locus_tag="VFO30_00685"
     CDS             27767..29986
                     /locus_tag="VFO30_00685"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HET9417831.1"
                     /translation="MKQRFSLLLALPAVVLLSAAMPQPLQAKSDAEQICVSVGRLLEE
                     GHYTHQPLNAAVSRKFLQTYLELLDYSHLFFTQKDIDALNTKYGEALGDDVLLGNLKP
                     AYEIFDLYAKRVDERVAKIKEFLKGPVDFKTDGSIDLSRQKAPWPKDEAEADELWRGR
                     ITNELLQEKLSEHPIEPGPQLVARRYDRLARTVHEEDRNEQVKLYLDALAQTYDPHSE
                     YLSKADLKNFSINMGLSLVGIGAMLRSEDGYAKIESLVPGGPAQTDGRLKVGDRITAV
                     AQGPAEYVDVREMRLDKVVEMIRGKKGTHVRLLVIPSDAADPSKRKSVELVRDEIKLK
                     DQEARADIIIKKDENGDPIKLGWLTLPSFYADMDRHSKSTTRDVLALLKRLKKENIAG
                     LVVDLRRNGGGSLEEAISLTGLFLKSGPVVQTKESNGSIRVSSDPDPGIIYSGPLVVL
                     TSRQSASASEIFAGALQDYGRAVIVGDHNTFGKGTVQTILELGRFTSLLGSRSQEDGA
                     LKLTIQKFYRVAGGSTQLRGVESDIVLPSLTDLHEFGEGALKNCLPYDEVAPVRYTKW
                     SDTHSLFIDQLKGRSAERVQNMTEFHYVMEDVARLQQKIKDNRISLNEDARRKELEED
                     KIRKETRAKERLARNDEEPRIYRLTLDTVDKPNLQLIMYPGKVAEAKAKGEPTKVAPE
                     AAPDDESELIGAEDDTKEPVIDPERDESLNILADLVDLSRGPKTASANSSKSPAARP"
     gene            complement(30065..30730)
                     /locus_tag="VFO30_00690"
     CDS             complement(30065..30730)
                     /locus_tag="VFO30_00690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9417832.1"
                     /translation="MRVLGGIACRAIAVGLAGFTFCSVAASVLAQDVNPFRVEIDLGQ
                     QTAYLLRGRYVALESPISSGRYGHLTQTGSFTVIEKERKHYSSIYGKIVDAYGNTVVA
                     DADVDMKVPSGCKFVPAPMPWFMRFHAADGMHAGYLPGYPASHGCVRMPEDLAIAFYN
                     AVDVGTPVTVFGRTPRTNGYFRYGPQPQRTFRPWFGQPMPPIYDGRFPPGMDPRHVEP
                     WWR"
     gene            complement(30948..>31292)
                     /locus_tag="VFO30_00695"
     CDS             complement(30948..>31292)
                     /locus_tag="VFO30_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET9417833.1"
                     /translation="LKPVTFRYNKEIDPEGIPQFGLVAEDVEKVNAALVTRDADGKVY
                     TVRYEAVNAMLLNEFLKEHRKVQELETARTEEQKEIAELKQQLHEQSEAIRKVSERVE
                     LPRPAPQVVDNR"
BASE COUNT         6587 a         9097 c         9089 g         6519 t
ORIGIN      
        1 gcaaacgcat gctcttcaaa ttctccggca acagctggct cgggattcat cttggcatga
       61 ccggcaaaat tgacgttgcg ccgccgcagt ttcagccggc gcaacacgac catctcgtgt
      121 tgtaccagcg taaacgcacg ctcgtcttca ccgattcgcg ccagttcggt cgcattcgat
      181 ttcatcacgg gaccgatgaa cccgactggt ggaagagcga cgctcccgaa atcatggcgc
      241 gtgaattcga ccgccaattc gtcgatgaat tcttagatcg acatcgtaaa gcgccgatca
      301 aagccgccct gcttttgcaa aacggtttcc cgggaatcgg caactggatg gctgacgaaa
      361 tcctctggcg cgcaaacatt ctcccatcgc gccgcgtagg ctcactgagc aatggcgacc
      421 gcgcgagatt gcggcgcgca ataaaattcg tcgtccgccg ctcgcttgaa acactcggca
      481 aagatttctc cgacccgccg agaaaatggc tgattcatca acgctggagc cgcaccggta
      541 tttgcccacg tcatcgcacc ccactccgct acgccaccat cggtggccgc accaccgcct
      601 ggtgcccacg ttgccaaaaa taattgtcga tcttaggcga tcacgtggac gacgatctta
      661 tgaaaaccgg tggcgccgga gaacgggctg cgatcgggtt cccattcctg cactacgcct
      721 tcgccgtcgg tggcgcggac gactaatgtg taatcgccag gttcgttagg cgaccagccc
      781 tcgtgcgcgg cccagagcga ccaggcgaga tcgcctcccg catagtagat atcggcgtca
      841 tcccaagttt caccgtcatc gaaactcaat tccacgcgcg agatgccgcg atcgccgccg
      901 taggcgatac ctttgatctc aatcggtctc cgtaactcgc gaatcgagaa gcgagactcg
      961 tgatcgggaa tgtcaatgcg tgaccgcgtc ggtgtgatga aatccggtcc ccaaccttgc
     1021 gcttcgtaga agcctttcgc gtcattggtc gtcagctcga tgcgcgtgat ccatttcaca
     1081 tgtttttcgc caaagtaacc gggaacgatt gcccgcagcg gatagccgtg gcgatgcggc
     1141 aattccttgc cgttcatttc ataggcgagc agcgtcgtgg gctcgagcgc cttctcgagc
     1201 ggtatagtgt ccgtgtaatt gtccacgccg tggaggcgca cacgcgcggc gttcgccagc
     1261 ggcgcggccg ggtcgatcag atcgtgcaag ggcacgcccg tccagaccgc gttgctgatc
     1321 agtcccgcgt cgagtccgtt gctgatgcac attaacgtcg tttcctgttc cgtcgcagcg
     1381 aaccctttca agtcacggaa gcggtacgtc gccgggttct gcacgaggcc gttcacttcg
     1441 aggtgccaaa catcgacatc gactcgtggg tcgatgacgt ttttggttac gcaataaaac
     1501 aactcgttag gcgtgatcgg ctggacgatg cggcctttgt actgcgtgcc gtcgtagctg
     1561 aatgtggcgg cgcgataaag tcggcgcagc aatgccgcgc cgccgccggc gagcactaaa
     1621 ccaactccgc cgaggatcaa cgcgcgtcgc ccgatcgccg gactaaattc ggcagcttca
     1681 tcgtcgatct tgcgctgcgt gagaaagcga aaagtggcga cgaggatccg ttcaaacacg
     1741 aacaccgaca gtgcaaaccc gagtagtgtc accagccgcg cgacgggaat cggaaatccg
     1801 ccatagctcg tgccgagcac cggccaaagt gcgatcgtga ccgcaatgat cggcagtaga
     1861 acgaacacgc caatggtgat gagcgtcaca cgccgcgccg gatcgcgtcg catgaacaaa
     1921 ccaaaaatcg cgccggcagt cgctgcgacg agaacttgcc cggctatagt cgagccaacg
     1981 ccgagctgct tgagatgatt gtagccgccg acttttccca tcagcgcgag aaacggtccc
     2041 ggtggaataa agaccgagag ccgatcgcca atgatcgcaa gtggcgtcgc gacgccaaaa
     2101 cacgcgagca acagcatgac aacagtcatc gcaattcccg cgacaagccc ggccagcaag
     2161 ccggcgaaca gcgccttcga tcggctcatc gattgccgat cttacaaggc gtggacgcga
     2221 tgtgaaccgc aaaaggtgga tcgagctctc cgagctcgat gcttaagtgg cggcgaagcc
     2281 gcattattta aacaacgcgc tcggagagcg cgttccacct cagcttcgtt ctaatgcgat
     2341 gcgaaccagc gtcgcgataa tccgcgcgcc ggcgacgaag gtgccgcgca atgtgccgga
     2401 gaccttcgag atgccgccgg cgcgacagcg atggtttacc gggatttcca aaatacgcag
     2461 accggcgcgg gcgacgcgca tctgcatctc taggttccag ccgtaggttt tttcgcgcat
     2521 gccgagtttc tcgagcgcgt cacggcggat tgcgcggaag ggcgacatgt cggtgtaacg
     2581 gacgccgtac aagatcgaca ataaccaccc ggccaaacgg ccggcaaaaa tctgttgcag
     2641 gttcatgctg ccgggctccc gcttcccgcg ggtgcgcgag ccgattacga agtcgtgcgt
     2701 accgcgcgcg atcggttcga cgagctgggg aatgaattcg gggcaatcgc tgccgtcgcc
     2761 gtcgagaaaa acgactacct cgtttttgcg ggaaactgcg cggactccgg cggtgcaggc
     2821 gcggccgtaa ccgcgcgtcg gctcgctcac cacgcgcgcg ccggcttcgc gcgcgcgctc
     2881 ggctgtccga tcggtgctgc cgttatccgc gacgaggatt tcgttaggca caccggtcgc
     2941 gagacattcg cgcacgacac cggcgatcgg ctcctcttcg ttaagcgcgg gaataacgac
     3001 cgagacgctc attccaacac cagccacgcg ctggtgaagt agatgaccag cgcgaagaaa
     3061 tcggcgagcg cgagcgtgat tggaccggcg gcgatcttgg ggtcgagctt caggcggtga
     3121 agaagcgacg gcacgccgag tccgaaaaga caggcgctga cgagcgaaag cgagatgctc
     3181 ccgccgatga cgcatgcgcc acgcgcgtcg tttcgccaaa gccagacgat catagcgacg
     3241 acaatgccgc aacccaggcc aagcagagct gcggtggtca gctcgcgtcg cagcgcgcta
     3301 acgaaccagc gccaggtgac cgaggcggag cgcaacgtct ggatggtgac gctcatcgac
     3361 tgcgcgctca cgctttcatt gaggccgagc accatggtga ggaagaacgc gatgacgaga
     3421 ctgcgcgcga gggtcgcttc gaaagcgccg gcgagcagcg cgcagattgt gccgctggcg
     3481 acagtggcga gcagccaggg aaaacgataa cgaaagactc gccatggcga ggcgccgcgg
     3541 atttgctcaa gatgaaagcc gagtgcttcg aaaatatcgt cgtgcaccgg cgcggccgga
     3601 cgatgtcgat cttcgctttc gcccgcctgg agcatttcct cggcaaaaag actcacgtca
     3661 atcacaccga cgacccggcg ttgctcgtcg atgacgggaa aggcgaagaa tttgtagagg
     3721 acgaaaaact cgcaggcatc gagcacggtc gcgctggcgg ggatcgcgac gacgcggcgg
     3781 accatgatgt cgcgtagcgg cgtctcgaga ggcgcggtca gcaaacggcg tgtgggcagc
     3841 actccgacga ggcgtttgtc ggcgtcgacg gcgaaaaaat agatgacgcg ctctccgatg
     3901 ccttcgcgcc ggatgcgctc gagcgcgtcg gccacggtgc tgtcggcttc gaggaggggg
     3961 aagtctttgc gcgcatgttc gacgacccgc gactcgagat cgggaatgcc agccagttca
     4021 ggcattcatc acttctacgc ggtgaacgat gcctttgctc ttcagaatct gttcggcttc
     4081 gctgaaataa tcgcgcacat cggtctgcgc tttgatccag tcgatgtaac gctggatgcc
     4141 atcggcaagc tcgacctgcg cttcgtaacc gatcgaccgg atgcgcgtga tgtcgctggt
     4201 gaggtggcgc atttcgccgg ggcgaaactc gccgcgcgcg accggttcga tgtcgatctt
     4261 gagcgcggcg gaaattgtct cggcaatttc gcggatcgga acgccgcggc cgctgccaac
     4321 attcacagcg aatccatcga gcttgtcgga ttcggcggcg agcaggttcg cgcgcgcaat
     4381 atcttcgacg aatgagaaat cgcgcgtctg ttcgccgtcc tcatacagca ccggcggtag
     4441 attgttaagc aagcgcgtgc agaaaatcgc gatgacgcca gtgtaaggat tgaacatcga
     4501 ctggcgcggg ccgtaggtgc acgagtaccg cagcccgacc gtgggaatgc cgacctgttt
     4561 gccccaaagc agcacgagct tttcctggtc gactttcgtc agaccgtaaa cggtttcacc
     4621 gccgatcggc gcgtgctccg gtgtcggcac actggtcgtg acggcgccac agattgggca
     4681 atggacctgc caatcgcctt tgcgcagttg cgcgacggga cggaccggcg ggaaaacgag
     4741 gccgtgcttc cggcattcgc cagcgccttc gctgtaaaca gcttgggaac tcgcggcgac
     4801 gatctttttg attggcagat tcttttcacg aatgagctcc agcatctgcg ccgtgcctaa
     4861 cgaattgacg tgaacgtatt tcgcaatctc cggcatgtaa ccgccgtagg ccgcctggtg
     4921 aaagatgatg tcgacgctgt cgagcgcggc tgcgagcgtg tcgcgatcgc gcatgtcgcc
     4981 gtgcacgaac tcgacgcgct catcgatcca cggcggcttc ccgcgtttgt gcgtttgcgg
     5041 ctcgagattg tcgagcacgc gaacggccca gctttcgcgc acgagaagat cgacaatatg
     5101 tgagccaatg aggccggcgc cgcccgtgac aagcgcgcgt tttgtcatgc ctctttttta
     5161 aaagcgcgtc tcattacgcg caaatccgtg agcgaccttc gcgcgcgacg atttcgctga
     5221 ggaattctcg ggtcgccggt gcgacgtcgt ttgtcgatgt tgcacgagtg tcgagaagct
     5281 cctggcacaa gcgccgcaac gtcgcgccgt catccacgtc gtagccgatc ggcagcaact
     5341 ccacccgaac ctgcaactcg cgtgcgcgtg cgatcgtttg ttgtagcact cgttcggtgc
     5401 tccaatcgat attctcgaat acgcggcggc agagtcgttg caggccgatc aagtaatagc
     5461 cgccgtcatc tgatgggccg agcaaaatgc gcggttcatc tgtcgacaac agcttcggcg
     5521 cctcggcgta cacttcgacg ggaaccatgg ggctatcaga atcgatcagg caaacggacg
     5581 agaagccgat gcgaaagaga tcctcggccg cgttaatcaa ccgcgcgccg aagtgatctc
     5641 cgcgctgcgg gagtaatgcg aaatcaggcg gcaacatgtc gtcgtaatcg gattcggcgc
     5701 cgacaggtgt ataaacggcg atcccgcatg caacgcttcc tgctgccgct gagatagcag
     5761 cagtagtatc gcgcaggaag cagctattta gctgcgcggc ttcgtcgggg gtaagtggcg
     5821 gaacaagccg cgttttcact tggccggcac gaggggcttt ggtcatcaca gccagcgcac
     5881 aaagtcctcg cgctgcgggg tcacatatct gtcgatcgag gacgcggtga accagattca
     5941 tcctgcacca gcgtctttgc cgatcaacgt ttttcgcaaa gcgcgtactg gtaacgtccg
     6001 tcgtgccacg tcttcacgcg gcgccatggc tgtgacgaga agagtttttc gaatgttgtt
     6061 ggcgagaaaa ccgtcgcctt tcgcggctcg cgataaccgt taccggaatg agtcatacgc
     6121 gcgagtagcc acatcgctgt gagtgggatc gacgcccaaa gcgcgtagcg cggctcggct
     6181 atgaaacagc ggcctcccga cttgagcacg cggtgcatct cagcgacggc ccgcgcgcag
     6241 tccggcaaca ccgtgaagag gcgcgacgcg agcagaacgt cgaactgagc gtcagtaaaa
     6301 ggcagggtta acacattgac gcgcacgaac gcgcagttgc gcaagctttc ggtctcggcg
     6361 cgttcccgag cccacttaag ctgattcgcc gaccgatcca caccggtaac ggaaatgttt
     6421 ggaaagcgtt tcgccagatt ggcggagtaa aatccgggcc cgcagccgag ctcgatgact
     6481 tgcgtgccgg gctgtgcgcc gccgttcggc cagagcgttg cgactatccg ctccgtatcg
     6541 tctcggaaca accgttcacg gcaaaatgcg tacacccagg caacgtgttc gaaaaggctg
     6601 tcgcttggtc gcgccgccgc gggtgacctt gaccggagcg gcttctcggt agcaacgata
     6661 atttgtggcg gcatttgttt aactcctgat tcgagcgcag ccgctggatt tttcacaagc
     6721 tgaattattg ctctctatct aatcggaccc ttcttcatca tcatcacact gcgaataagg
     6781 tcgattccca tgctggcggc tcaggcaaac gggcctgggc caggttgttg ataacagctt
     6841 caataagggc cgacaaccac cgagtgcgga aagtggacga aagtttttcg ccattattcg
     6901 aagttgttgg cgtgttgctg tcgaatgcct taaacaaggc cgaatgcaaa ccagaacgac
     6961 gtctcttttc tgcgcgactg cgctttttac ggttgtggtt tcttccgccg gcgccgcgga
     7021 gctaccgcca ctttggaaaa tccagcaaac cctcgctgcg aaacgctatg tcgatcttac
     7081 gcatgcgttc acgccaggga ttccgcgctg gcccgggttt cccgatgaga cgcggaaaac
     7141 aatttactgg tacgacaagc ggcccgacac gatgggcgcg ggcttttttt ccgagctgtt
     7201 cacgcacgtc gggcaatggg gcacgcacgt ggatccaccg gcacatttca tcaaaggttt
     7261 gcggaccgtc gatcaaatcg atccaaagga aatgatcttg ccactggtcg tggtggatgt
     7321 gcatcgcgag tgcgagcaga atccggacta cacgttgacg gtcgaccggc tgaaaaaatg
     7381 ggaaaccgag cacagccaga ttccggccgc cgcattcgtc gcgatgcgca ccgattggtc
     7441 gaaacgctgg ccggacgcgg cgaagatgga gaataaagac gcaaaaggcg tcgcgcatta
     7501 tccgggctgg agcttgcccg cgctgaaata cctctacgaa gaacgcaaga ttaccgcgtc
     7561 agggcatgag acgactgaca cggacccggg cgttgcgacg acgaaggagg actactcgct
     7621 tgagacttac attttgagca cgaatcatta ccagatcgag ctgctcacga acctcgacca
     7681 agttcccgag gccggcgcgc tggtggtcgt cagttttccg aagccgaagg atggctccgg
     7741 ttttccggcg cgggtgtttg cgattttgcc ctagcggcgg gccctgactt cggtattggg
     7801 cgtggatgtt gcgcgtggac cgtttgcttt atctcacctg acgtaagtat tcccgatccc
     7861 aagcggccgg gatgctctcg cgtgcggagt acgggcctgg ctggtaggcg cgcgaggcaa
     7921 tgccctgctg gctcaactcg cacacgtgcc agtcctgctt gttcgtcatg tcccagaagg
     7981 caatcgcgtc ctctggattg aaatctcggc ggtcgaatgc gtcgcgatga aaaaaccagt
     8041 cgcatacaat gcgcgtatga tccggcgcct gcggccagag ctgatgcacc atgacgtatt
     8101 ccggatgcag actcagcagc atgttgggga aaatcgagta ataaaagacc cgctgaagat
     8161 ttttgatgtc cccgacgaat gacgcgcaag cattgccgct catcgtgagg ctttggccct
     8221 tattgatcga catgaatcca ccgaggaacg ggccttcggc cagatcgttc tccgccgaat
     8281 cgtacggtga aatcttagac aacatcggat gcacgcccgg gcagtggtag cactccgaat
     8341 agttctcgaa catcaatttc caatttgcgc gcacgtcgta ttcgatgtgc cttgcggggc
     8401 gcaagatcga catgttccag tgcgagaatt tcccggcgag cggcgcgaac cattcctcaa
     8461 gtgacactgg cgttttcccg aggtttacaa agatgaagct ttcccacact gccaacttca
     8521 ccggattcaa cgaataatca gccttattaa atcccggcac ctcatccatg tgtggcgcgc
     8581 cgatcaaccg tccgtcgagc ctgtacgtcc aagcgtggta tggacactgg atcgccgaca
     8641 ggtggccttt tgctttttcg cagagccgtg tgccacgatg tcggcacacg ttgtaaaatc
     8701 cgcgaattgt cgatctttga tcgcgagtga tgatcaaaga ctcgctggcg atgttgacga
     8761 caaagtagtc accggcttgt ggaagctggc tctggtggcc gactaacaac cagtggcgcg
     8821 aaaagatctt ctcctgctca atggcaaaga cctctggcga tacgaaatat cgttgcggca
     8881 acgtcttcgc gcccggcgca aacgtctcgg cggttttgcg gaatgcacgc gccgccgttg
     8941 tctgatggag caccgacatg tccagatcgt tgcatgcact acgcgtgtgc gtcaacggac
     9001 caactgatgc taatacggtg gtggttggaa acgtggcgcg agcagtttga ccgaaagtcg
     9061 ctgcacgaac gaaggtagcc gttgaagcat gcacatcacg gagatcatgc ggagggattc
     9121 tccgacattt tcgttcgagt tctttccacc gaaaacgccc gaggcggccg agacgctcta
     9181 ccaaacgatt cgcgatctgg aatcgtacat gccgcacttt gtcagcgtta cctacggggc
     9241 tggtggttcg acgcgagatt taacccacca cctggtcgag cgtattcaac atacaacgaa
     9301 actggatccg atcccgcatc tgacctgcgt ctgccacaag gaagaagaga tcgacgcgat
     9361 tttagctcga tacgcaaagt cggcgattgg aaatattctc gcactcgggg gcgatcggcc
     9421 ccgcgacatt ccctacgata aatcgcgcga ttcgttccaa catgcggtcg acctcgttag
     9481 gttcattcgg cgattcaatg agtccggtgc gcatccggac cgccgcgggt tcgggatcgg
     9541 cgtggccggt ttcccggaag gacatccggc aacgccaaac cgactagtcg aaatggacca
     9601 cttcaaagcg aaagtcgacg ccggagccga ctacgttgtc acgcagctat tcttcgataa
     9661 ccgggatttc tacgatttcc gcgagcgttg tgcgctcgcc ggggtccaca tcccgatcat
     9721 tgccggtatc atgccgataa cgtcgatgag cggaatgaaa cgcatcgcag agctggccgg
     9781 tggcgcgcgc ttccccgcca ggcttctgcg ggctgtgcag cggtgcgaca acgatccgga
     9841 gatggtccgc cgcgtcggcg ttcacttcgc cttggagcaa tgtcacgacc tgttggataa
     9901 caacgtggcc ggcattcact tttacacact caatcgatcc gacgcgacgc gggtgatttt
     9961 tgacagtctc ggcattccgc gccggcggag agaaggggcg cctaccgctt tatcgtccga
    10021 cgacattcac cagcggctgg caaagtgacc gaccacgagc gtgcgtcttt gtgatctggg
    10081 cgtgaactat tgatcttgga atttgcgcca ggcgtttgag ccgccaaatt tcctgatgct
    10141 gaatacccgg cgcgcggtgt acgtccaggt tggcggggtc gccctggcgc tggcgctgtt
    10201 gcttttgctc tcacgctttt tgccaattat cgatcttatc gaacggtcgg agaacgcggt
    10261 aatgcactgg ggaacgtgga gcatggtcgg ttatccgtta cttttcgcgc tttgcaatct
    10321 tttacttttg ccaggaggat tgttgagcgt aggcggtgga ttcttctttg ggttgtggcc
    10381 tggttttctc ctcgtgttgc ttggtaatac gatcgcggcg gccacctcat tcgcgctcag
    10441 tcgctgggtg gggcgccgct ggtttagcca gcgatttgtg caaagtcgtg tactgcagct
    10501 tctggaaccc gccgtggaac gcgaagggtg gaaaattgtc gcgttgagcc agctccaccc
    10561 gatgttccca actagtttgt tgaattactt ttatggccta acgagaatcc gcttcgctgc
    10621 atgcatgctt tggacgtgcg tgggacgagt gccgggcctg ttcctatatg tttatcttgg
    10681 gacattaggg aaggtcgggt ttaatgtggc gcgcggtgtt caccacccac acatcgctga
    10741 atattggaca tgggtcgcgg cactcggtgt ggcggtgctg cttgtgattc tgttaacgcg
    10801 catggcggtg cgagagatga aaatggcaga agaggtcgaa actgccggta acgaatccga
    10861 tccgcagcag gtcatcaatg cataagtaac tccgccaatg ggttttatag tccgcacacc
    10921 cgactttcag ttgagcgatc ttctaagacc gcaaaaagga atgctggagg gtctacttga
    10981 tgtttaagct gccgaccgag tacgatgtcg tggttgttgg tagcggccat gcgggcattg
    11041 aagcagcact ggcggcagcg cgcctgggct gccgaacact aatgctgacg caaaatttgg
    11101 acaccgtcgg gcagatgtcc tgcaacccgg caatcggcgg attggcgaaa ggacatatcg
    11161 ttcgggagat cgacgcaatg ggaggcgcga tgggcataaa tgcggatgcg accggcatcc
    11221 agtttcggat gctcaatcgc tcgaaaggtc cttccgtgcg cgcaccccgc gcgcaatgcg
    11281 acaagaaagc atatcagttc cggatcaagg cagtgcttga acgcgccgcg aacttggatc
    11341 tgaagcaggc gaccgtatcg cgagtcatcg cgcgggacgg atgcgtcacg gcagtcgaga
    11401 cggacatcgg cgtaatgatt cagacccgtg ctgcggtaat cacttcgggc acatttctgc
    11461 gcggcctgct tcatgtcggt caaaccagta aacccggtgg gcgaatggcg gatgccgcct
    11521 ccgggctaag cgacaatcta cggcagctgg gcttccaagt gggtcgattc aaaacgggaa
    11581 cgccctgccg gctgcaccgg cggtcgattg atttttccaa gtgtcagata caactcgggg
    11641 atgaaccgcc accgcgattt gcgtttttgc cggaggtcgg tgctgggcgg aatgatattt
    11701 ttacgctcaa tggcgaacac gccggggtgt tccacgtaga acagttacca tgttgggtga
    11761 cccaaaccac cccccggacg cacgagatca tccgcgcaaa tctgcatcgg tcgccacttt
    11821 atgccggccg aatcaaaggc accgggccgc ggtattgtcc ctcgatcgag gacaaggtgg
    11881 tcaagttcgc cgagcggtct tcgcatcaca tatttctcga gcctgaaggg cgtcacaccg
    11941 aggagtttta cgtcaacggc atctcgacca gcttgccata cgacgtgcag atcgagttcg
    12001 tccactcgat tccagggctc gagcgggcgg aaatcatgcg gcccggttat gcggtcgagt
    12061 acgattactt cccaccgacg cagctattgc acacgttgga aacaaagctg atcgtagggt
    12121 tgtattttgc gggtcaggtg aacgggacct ccggctacga agaagccgcg gcgcagggct
    12181 tgattgccgg ggcgaacgcg gcactgaagg tgcaaggtcg acaaccgctc attctgcaaa
    12241 ggaataccag ctacattgca gtgctgatcg acgacttggt gacgaaaggt acggaagagc
    12301 cttatcggat gttcactagt cggtcggaag accggttgca gctacgccaa gataacgcgg
    12361 atcagcggct gacgccgttg gcctcgcggt acgggctcat cgatgaagcg cgatcgagtg
    12421 tattagggtc caaaatgcag ctattgcagg acgcgcgacg catcgcagcc gaagcaaaag
    12481 tgcaaggcgc accgatcctt cagctgttaa agcggccggg cttcactttc agttatttgc
    12541 cggcggaaat cgcgcgcgca gttccggcgg aaatctggga gctgctcgaa accgacctca
    12601 agtacgaggg ctatgctgcc cgacagcgcg accataaccg ccaactgacg tgtcgagagg
    12661 ctcagccgat ccccgacgcc gtcgatttcc gcgcgattcg cggattgagt ttggaaacac
    12721 gccaaaaact ttctgcgata cggccgactt ccctggggca agcggctcga atcagcgggg
    12781 tgactccagc ggatatatca attttgtcga tatggttgag cagcaaagga ttacagttaa
    12841 gtaatcagcc aagatcgaca atctactcgc ccgacacata gatcggccaa agaggttcct
    12901 cgtgagcgct tatgctacag tcgcggtgct cgggtatctg atcggctcgt tcccagccgg
    12961 ctacgtcgct gggcgaatcg caggtgtcga tattcgcacg catggcagcg gcaatatcgg
    13021 cgcgacgaac gtgctccgag tgttggggaa acgattcggc tatgcggtct ttctggtgga
    13081 tttcggcaag ggtgttgcgg cggttctggt ggccagacat atcaccagcg ttaacggaac
    13141 agatgaacgc gcggttgaat tggcagctac gctggccggc attgtcgctg taatcgggca
    13201 cagctttccc gtatggctta aattccgtgg cggcaagggc gtcgcgacat cgattggggt
    13261 cctgtttgcg ttacttccct tggctggcgc cgtcgtgtgc cttgtctggc tcgcaacgtt
    13321 tgaaattgcg cgctatgttt cactggcatc ggtcgttgcc gccgtcgcac tgccaattgc
    13381 cgttgcagcg atgttctttc tgcatcgact tcagacgccg gtactactgt attgttcact
    13441 ctgtcttgct gcgctcgtga ttgttcgcca tcgttcgaat ctggcgcggt tggcgaaggg
    13501 aaccgaacca cgcttcagcc gaaagtgaac ctccagaaac tggctattat cggcgccggt
    13561 ggctggggca cggcactggc cgttctttgg gcaaagcttg ctccggaggt tgtgctgtgg
    13621 ggcaacagtg ccgcgcgcgt cgagaaaatg cgagcgacgc gcgagaacca ggtttacctg
    13681 cccggcattt gtctgccgga ttcgcttggc ctgaccagcc gactcgacaa atgcagcgac
    13741 gccgatctca tcgtgttcgt tgttccatca actgcattgc gagctattgc cgaccgactt
    13801 cgcgatatcg ctggcagcaa tcacgccgtt cttctcactt gcacgaaagg cgtcgagcat
    13861 ggcagcggaa tgcgcatgag tgagattctc actcagattt tccctgagcg ggcaatcgcc
    13921 gcgctgtccg gacctaatct ggccgcggaa gtggcgcgcg gcttgcccac tgcgaccgtc
    13981 ctcgcgtgcc gccagccgga atacgcgcgc gagttgcagg gcctgctcgg cagtccccgc
    14041 ttccggattt acacgggcga cgaagtcccc ggtgtcgaac ttggcggcgc attgaaaaac
    14101 gttttcgcca tcgccgccgg cgccagcgaa ggtctcggcc tcggcgacaa ctcaaaggcg
    14161 gcgctggtaa ctcgcgcatt ggcggaaatg atccgtctcg gcacggcaat gggcggacag
    14221 ttgaggacgt tttatggcct gagcggcgcg ggcgatctcg tgttgacctg ttacagcaaa
    14281 caaagccgca accgcagcgt cggcgaacgt ctcggccgtg gcgaatcgct ccgacaaatt
    14341 acttcgacga cgcaaatggt cgccgagggg attccgaccg cgaaaagtgc gtacggatgc
    14401 gcgcggaaac ttaagatcga cacgccgatt atcgaccagg tgtacgcgct gctctacgaa
    14461 aacaaaccgg cggtacaggc gctggaggac ctgctcgggc gcgagcaaaa acccgagcgt
    14521 gtctaagcgc gcgactctgc cgcgcagaac tgcgcgatga ccgctggata caacttgtgt
    14581 tcagcaatct ggattcgcgc gtgcaaactt tcctcggtgt cgttaggcag aatcgcgacc
    14641 tgacgctgag cgatgatatc gccgtgatcg acttgctcgt cgacataatg cactgtgcag
    14701 ccggtcactg tctcgcccgc tgcgagcgct tgcttccacg cttctagtcc ggggaacttt
    14761 ggcagcagtg acgggtggat gttaatgatg cggcgtggga acgcctggag catcggttgt
    14821 ttcaggacgc gcatgaaacc cgccagcaca accagttcga ctccggcgtc gcgcaacatt
    14881 tgcaccagct ccacttcaac tcgcggctcg aggcgggtgc gaaattggcc tggtggcaga
    14941 taagcgttcg gcaccgtgaa ttcccgcgct atctcgagaa ttggcgcatc caacacatcg
    15001 gagatgacga cacgcgcttc ggccggcagc acgccagagc agatgctttc gaggataacg
    15061 cggcagttgc tgcccttgcc cgatccaaga atgccaagtc caagcggtct cattctgccg
    15121 cggtgcgcag cttgttgagc aattgcgacg tcgagtaacc gtgctcaaac ggcacgatgt
    15181 cgatcttagc gccgagtttc tccagcagcg cgctctcctc cgcgtccaag ctgtcggacg
    15241 tgtaatcgcc gcctttgacg taaacgtccg ggcgcacggc ttggatgaag cgtgtcgccc
    15301 gcggctccgg aaaaatcgtc actaaatcaa cactttcgag cgctgcgagg acttcagccc
    15361 gatcatgctc gcggttaatc ggtcgtcctt cgcctttgag cgcacgcact gaggtatcgg
    15421 cgtttagtcc cacgaccaac aagtcaccga gactgcgcgc ccgttgcaga taacgcacgt
    15481 gcccgacgtg gagcaggtcg aaacagccat ttgtcgcgac gacctttttt ccggcttcgt
    15541 gcaactgttt cgcgcgctcg gagatttcgt caaacgcgat aattttcgag tgtttcattc
    15601 cggcacgaca aagtttagcg cgcgcgggac gacttcaaaa accgccggca cggttccgac
    15661 cggttcgcca tcgacattga accacatcgc tggccgcgaa cgtaccgcaa tcttcttcgc
    15721 ccgccggaat tcaaccgcat tacttttgaa gtgtttgcct aacaaaattt ctgccgccag
    15781 gagcgccacc ttcgcagccg ggtgagttgg aatgagaacg atgtcgagca aaccatcgcc
    15841 cggatcggcg ttcggtgcga ttggcagtcc gccggccaca aaacgtccgt ttgcaacgac
    15901 gatgttgtaa agatcaaccc ggacgacctc gctcgagtcg aattcaatgt gagcgtgata
    15961 gacgtgcagc ttcggcaggg cagcggcagc gctgcgcaaa tacgcgagcg gcccccatgt
    16021 ttgtttcata tctggggtca actgttcgtc aacgacttcg ctaaacccac cggcggaaac
    16081 gttgacaaaa taacggcttg gccgactgct ccgcacgcga acgatgtcga tcggttttgt
    16141 ttttttcgaa agcaagatgt cgatgttgtc ctcaactgca gtcggcaaat taagtgagcg
    16201 cgcaaaatcg ttcccggtgc caagcggcat tagtccaagg caaacccgac cagcacacga
    16261 tgcgacgccg ttgacgactt cgttcagtgt gccgtcgcca ccagcagtta tgatccactc
    16321 gtagttgttc cgaagtgctt cgcgcgcgaa ccgctgggca tcgcgggcct tgcgcgtcat
    16381 gcggataact gcaggatcaa gtcggttcaa ttgcgcgatg acagcatcga tatcgccgat
    16441 cgatccggcg ttcggattga aaatgaccca cggcttcgcc gattttctcg agcgcgctga
    16501 tcggttgtcg atcttacgac tttggtcacg cagcattccc tttgcttttc gtcgattttc
    16561 gccaagttgt cgatgagccg cgatgccgcc ccctaattcg aaatcacctg cggaacccga
    16621 ccgtcgcgac ttccgcgttt cccgtggcag ccttgtcact ttagccgaaa cggagcgcgt
    16681 cgattttatt ccgaacgcgg tggagttgcc gcgcttttac ggcgaaccgc tcctcttcgc
    16741 gatcgggcgc gatccgcgca ccattttcgc ctactggagc atcgattggg cgtccatttt
    16801 cgacaccacg gcgccggtag atcgacaagt acatctgcgc gtttatcgcg atgacggcgc
    16861 tgaagagaca gccgccgccg ccgagccgat ggcggggaat tgctacatca cagtttcgca
    16921 gccgcgcgcg agataccgcg tcgaactggg ctattatcag ccgaaaaaca gctggcacgg
    16981 cattgccaca tctgaggaag taacgatgcc gcccgacagc gtttcggaag ccacggatgt
    17041 cgatcttgcc acaattccgc ttcacttgag ttttcaacgg ctcatcgatc tcttccgcgc
    17101 gaataatagc gacgcactca ccgagatcat ttcccgtctg caacgacgcg ccgtcactgc
    17161 ggccgaacgc gacctgctca cgcctgagga actggaaatc ctccgcgcca tgaatgtttc
    17221 gctggaagaa atggaatccg gccgtgcttt gttccatgac agacaaatga gcgaagccgc
    17281 gctccgccgc cgggccgaaa cactcctcgg ttttggggcg agcagccgcg gacacgcatt
    17341 tagcgcgtcg agctggggct gaaaccaaac tagcgggtca gcttcatacc ctcgggcaac
    17401 tccggcggag gtgccgtgat ccggctgtgt tgtttgccgt aggtaaagta gaacacgaga
    17461 ccgataagga gccagacgat cagacgcatc cagttcgtcc agccgagccc gtagatcatc
    17521 gcgccacaga cgatgatccc caagattgcg accaccggcg cggccgggac ccgaaatgcc
    17581 cgctctagat caggacggcg aactcgcatg atccagacgc ccgcgcagac gagaatgaaa
    17641 gcgaagagtg tcccgatact cgtcatctcg ccgacgacat cttcgggaat gaacgccgca
    17701 aacaagccgg tgaacacgaa aaacagcatg tttgatttgt aaggtgtcct aaatcgcggg
    17761 tgcacctggg aaaaagtctt cggcaccagt ccatcgcgac tcatcgaaaa gaaaacgcgc
    17821 gattgaccaa gcagcatcac gagaatgact gacgagaaac cggcaaggat tgcgacggtg
    17881 acggacttcg acagccattc gaaaccaggc atatattttg taatcgcgaa tgcgactgac
    17941 gcttcgcggc cggccgtacg gaagtcctgc acactcgcga cgccgctcag cacgtacgaa
    18001 aacagcacgt agagaaccgt gcagatggcg agtgatccca gaataccgat tggcatgtca
    18061 cggctggggt tcctcgcttc ctgcgccgct gtggatacgg cgtcgaaacc aatgtaagcg
    18121 aaaaacacaa cgcccgcggc gccaaggata ccgagaatgc cgccatacgc gtgctgaatg
    18181 ccttgtggcg tgacataagt cgtcggcgcc ggaatgagcg gcgtgtggtt cgcaggattc
    18241 atgaagcgcc acccgatgcc aatgaccagc agcacgatgc tcaccttcag aatgacgatg
    18301 agaccattca caaaggccga ttctctcgtg ccgcgtatca gcaggagcgt cagcaacagc
    18361 agaatgaaca cagcgggaat gttcatgatc ccgtgaatcc cgctgccgat gtcgtgctcg
    18421 aacggagagt gcgaccactg gtacggtatg cgcaagccaa accagccgag aacgcggtta
    18481 gcgtactcgc tccacgcaat cgcgaccgtc gcggcgccga ctgcgtactc gagaatcagg
    18541 tcccagccga tgatccaggc gacgaattcg cccatcgtcg cgtaggagta agtgtaagcg
    18601 ctgccggcga ctggaatcat cgacgcaaac tcggcatagc aaagcccggc aaatgtgcag
    18661 ccaacgccgg ctacgatgaa cgcgagcacg accgagggac cggcgcggtc tgcaatcgcg
    18721 gcggcggttc gcacgaacag accggcgccg atgatggcac cgatcccaag catcacgagg
    18781 tttacgggcc caagcgagcg tttgagcgtg tgttcgccga cttcgtgcgc ctcttcgatc
    18841 agtcgatcaa gaggcttttt ggcaaatagt gacgccatcg atttaccttt cctccttcac
    18901 atcatgcttg ctgtggaatc atttcgtttc acgcacagcg ttcgcgctcg ctgctgtcat
    18961 cgcgtttcga ttttctccag agaaaataaa ccggcacgcc ggcgagcacg atgagcaaac
    19021 ccggccagga cgtctgcgtc cggtagacgg ccagcacaat caggatgatc atcgctgcga
    19081 caatgtagaa taccggcaac agcggatagc cgaacgcgcg atacggccgc tcggcctcgg
    19141 gacgtgtacg cctgagcacg aacacgccgg caattgtcag tacataaaaa atcagcaccg
    19201 caaacacgac gtagtcgagc aatgtgccgt atagatttcc gtattgcact tcaccggcgg
    19261 cgttgcgcaa acgcgtgcgc ggaagcacga gcaatgcggt ccacaccccc tgcagaacca
    19321 acgcaacggc cggaacatgt cgcttgttca ggcggcctgt cgatctaaag aacaatccgt
    19381 cttgcgccat ggcgtaataa acgcgcgcac cggcgaggat cagcccgtta caacagccga
    19441 acgtcgagat catgatggcc accgccatga tcgccgcgcc ggccccccca aacatcacct
    19501 gcatcgccgc cgtgcccacg cggtcatcag gcgcgtgctg gattttgtcc aacggcagca
    19561 cgcagagata ggcgacattg gcgagcagat acagagtaat gacgaggcca gtgcctaacg
    19621 cgagtgagag cggaatgttc cgacgcggtt ccctcacttc gccggccgtg aaggtgatgt
    19681 tattccatgc gtcggcagaa aagagcgatc cgacttggcc aacgcaaatc gcaatcaaca
    19741 tgccgagcgc accgcccgtc gccgataccg ccgcgatgaa ggagagatcc gatttgatcg
    19801 gcgagacgat ccccggcgtc cagaaattgg tgaaattggt ctggagcgcg tcggcattgc
    19861 ggccgacgaa tataccgagc acgatcagcg cgaagagcga caatgttttg gcagacgtga
    19921 aaatgttttg gatgagtttg cctaacgaca ggccgcgcgt gttcacgata gtcaggcaaa
    19981 cgatgatcaa aatcgcgatg agttgctggg tcgagagact gatcgcgtag ttgcgggaga
    20041 ggatgattgg cggaacgatc cagttcgtcg gcgatatcga tgcagcgaac acgcccagaa
    20101 aacgcgcgaa cgccaccgcc acagcggcga tcgttccggt ctgaataacg agaaaaagcg
    20161 tccagccgta gagaaaaccc cacagcggcg tgtacgcttc ccgcagataa acgtattggc
    20221 cgccggcccg cggcatcatc gcggccagtt cgccataact cagcgctgcg gttatcgtca
    20281 gcgcgccggt tacggcccat accaccagca accaaccggc cgagccgact aaccgggcga
    20341 tgtcggctga cacgatgaaa atacccgagc cgatcatcga acccgccacc agcatcgtgg
    20401 agtcgagcag cccaagcccc ctaacgaatt tctgttcagc tacaggtgtg tcggcaatcg
    20461 cggttccgga gttcatcctg tcggaaattt gggagagtta atcatcagcg acgcgcgtgc
    20521 ggcaatgtga aaactttaat ggaagctgaa gcgcgaacct gtttttcctt tcagcaagca
    20581 tgatattctc cgcgccactt cagcaagcct cgcatcattg tcgatctaaa gcgctggctt
    20641 gatggtgcga aaatgttcac tcttgcagct tacgcagtgc cagtcgtcgc agtcgtgggc
    20701 gtgccgctcg aatttgttct gttcggacta acgctactca gtctcgcgat ttttcaccat
    20761 cggacgatgc gcgtcgcggt tgcgggcctg gtgattatct cagtatacaa ggttgccttt
    20821 accggtttca aaaccggtcc gggcctggcc ggtttgtggg ttcatcttgc acaggagtgg
    20881 gtgatcttgg tgaacctcct ttgtttgtta acggcgttcg ccttgctgtc gcgacatttc
    20941 gagaagagtc atgtgcctgt ggtgctgccg cgctttcttc cccacgactg gaaaggcggc
    21001 ttcctgctgc tgctaatggt ttgggttctc tcgagctttc tcgataatat cgcggcggcg
    21061 ctcatcggcg gcgcgatggc gcaccagctc tttcgcgcca aagtacacac ggcgtttttg
    21121 gccgctatcg tggcagcgtc aaatgcgggc ggagcatgga gcgttgttgg ggatacaaca
    21181 accacgatga tgtgggtcgc cggtgtgtcc ccagccgctg tcttccgagc gatcatcgcg
    21241 gcaatcgtcg cgctgctggt ttttgccgtt ccggccgcaa aacgacaaca caactattcg
    21301 cccatcctgc agcacgcgca tgaacacacg cgcgtggact ggattcgcgt cggcattgtg
    21361 agttcgatgc tcgccttcgc gattgtgacg aacgtcgcga ttagcttgtt cttgccaaag
    21421 tcggccgatc gattcccttt catcggcgtg gccgtctggc tcgcgttgct gatttctgtg
    21481 ccgctccgcc ggcccgactg ggaattcctg ccgcggacca ttcgcggctc gatctttctg
    21541 ctcgcgcttg tgttgtgtgc gtcaatgatg ccggtcgaag aactgccagc tccgtcgtgg
    21601 cagacgacct tcgggctcgg cttgctatct gccgcgttca acaacattcc gctcaccgcg
    21661 ctggccctgc aacagggcgg ttatgactgg ggctttctcg cttatgcggt cggttttggc
    21721 ggctcgatca tctggttcgg ttcctcggct ggcgtcgctg tctgcacgct ttttccgcaa
    21781 gcgcggtcgg tcacaaattg gctgcgagat ggctggtggg tcatcgccgc ttatgtcatc
    21841 gcgttctttg ccatgttcac cgtgtggaaa tggcatccag acaagccgcg gccggccaag
    21901 agcgttccgg cagccactct gctaatgaaa tgacaccgcg tgccgcgcga acgctcatcg
    21961 tattgtcgat ctttgttatc gaagcgttgc tcgtttattt cctggtggcg cgacgtttcc
    22021 cattttccgg cgacgattat tcgtatctct atcaagcgag actcttcgcg tccggccgta
    22081 tttatgccga agatccgttc tacgatccga agctgccgtt ctatgattgc attcagactt
    22141 actgcctccg agacaatcgc ggccacaggt tctccaaata tccgcctggg tggccggctc
    22201 tgctcggtct cggcgcccgg gtccgggccg catggctggt agaccccatt ctcggcgcgt
    22261 tgctactcct tctggttatc aatcacgccg agcgacgctg gggcgaacac ttcgcgacaa
    22321 acacgtctct gctcctcttg ctttgtttct tcttcgttta ttacgcggca tcgttccggg
    22381 cgcatatcgc tacggcactg ttcatatttg ccgcgttttt ggtgtacgac gctaacgagc
    22441 gtcgcgcagc gcgttcgaaa ctgtggctgt ttctgagcgg agctctactc ggctattcgg
    22501 cgatgattcg atatatcgac tggataccgt tggcgctttg gaacgggtcg cacctggtgc
    22561 ggcgcaacag attcgtcgat ctcggtgttt tcgcccttgg gttttcaata ttggctagcg
    22621 gtaatctgct ctacgacgca ctgctcttcg gggatccatt gcaagcgccc gcgacgatgt
    22681 atcacttctc cgccggcatt aacgatcatc tatttctttc gtggcgcagc ttcacagtga
    22741 ctgcagcgcg gctcgggcgt ctgctcctcg tttttccacc gttgctgctg ttgatcacgg
    22801 cgttgagaaa cgtccaggtc tgggccgagg cgaagactta ccttggcctg ttcttgctta
    22861 gcgtagcgac ttactttttt taccccgaag ctgtcggcgg acccggtcct cgttatttcc
    22921 ttggctactt cccattcctt gtgctggcgg tggtggccgt ttaccggcgg atggttgagc
    22981 aggcgactcg tctcgcacgg cccttgtgga acgcagcctt gctcaccctg gttctgtgca
    23041 atgctgtgtt cattgcgaag gcttcctacg tcatgcacgg gcggcgcgac ctggagcgaa
    23101 cagcggaaca gctttcagac agaaacaaga agatcatctt cctcaaaacc ggcacgtatg
    23161 acacgcccgt cggcgatctg acgcgtaatc cgccagtact gtcatccgcc cgcacattgt
    23221 acttcaaatg gtgcgatcaa tcggacgttg ctgcgctgct gcagaggttc gccggccgca
    23281 gcgtttacgt ctatgaatat ccagggcggc tgcagcgata caaccggcgc tgaacaaagc
    23341 gagggttcta ctcgtgtcgc gcttgcgtcc agccagtccg cggcagacag ttgcgatcct
    23401 ctcttgggaa atgggcgtct ggaatcgttt cggtcgtgat cgcgtctgca ttgagcgcgt
    23461 gatgaacacg cgtcgcggcc gatcatcggc aacatgtggc ctgtcgatct tgctgttcat
    23521 ggcgttttgc gccggggaaa ctttcgccgc gccccgatcg cgcaggacac cctcacaagc
    23581 gccgccgaag ttcgaagcat tgccactcgt gcggtctcgt cagggccacc taatcgttga
    23641 agcgttcatc aacggaaaac cggcgttgct tggcgtcgac agcggcgcgc ccctcagcgc
    23701 gatcgcggag aatcggcgtc aatacttcgg gctcacgggc gtgagcggct cgaggttgcc
    23761 agcgcgactg cagattaacg gcgccttcaa cagcgtggcg atcgtccgcc gtttccgtct
    23821 cggagcgctc agtctgcttg accacccggt cgtggtgatc gacctggacg gatcgagccg
    23881 gtccgctcgc gtcctgcacg agcaggaaat cgacggcatt gtgggtgctg acattctctt
    23941 tccgactcag gctgtgctcg attgccagcg gcaactcctc attttgaaag tcgatcccga
    24001 tgttaccggc agcacgccgg ggattgagta tcgtggtctc cgcagcgtgc cgattcgcgt
    24061 cagcgaagat tacaatctct atgtcgacgg cgcgatcaac ggcgccccgg ctcagctcat
    24121 ggtcgacacc ggcgcgttcg ccactttgct gcaccgcgat ttcgttaggc gaatgcgcat
    24181 tccactgcgc gagacgccct ttaggggggc cgcggttaac ctgaaacagc gtggcgtgca
    24241 gatcgcgcgc attcgccggc tgagcgttgg ctcggtcgat attgtcggca aggatgtagg
    24301 cgtgatcgac ctgagcggac tcgttcgtca cggattgctc gaagcttcac cgcccgtcgc
    24361 tggtttactc ggcgctgaga tcttgcgctc gcatcacggc attatcgatt tcggcactcg
    24421 gacgctttat ctgaaacggt aattcgtctg ctagccgcaa tcgtcgcgtc ctcacctcaa
    24481 atcctctccc tgagggagag gaggacgcga cagcgccagg tgagggatta ctcgacatca
    24541 atttcgcgaa cgcctttttg ctggcgaata atttcgactt ggttgcgaat tttgtcggcc
    24601 aacccgacgc ggcgcggcac tgcttgaata aacacgaatg gtgacgaggt gtcggaagta
    24661 ttcagctgca cgttttcgat tccgaccact cgataaagaa acggctgccg cgtctcgaga
    24721 tctttcacgc ggtacaactc gagcgtatcg gtcaccttgc tcagaatgcc ctcgctgatt
    24781 ttaatgcgct cggttgtaag ctcgtacacc ttgcttttag tttgcagata tcgcacgagc
    24841 gcgagcaaca acggtagggc gagcagaagc aaaatgtacg gactgagatt tcgcatttgc
    24901 agcgagtcgg cgcgagaaag agcggcaaag atcaccagca cgacgacaga catcgtcacc
    24961 gccaaaatat accgcccaaa attcttccac tgcgacgagg tgccacgcca aacggtttct
    25021 tccggcatgc gcaattgtta gtccgatgac ggcgatcact caagcattcg tcctcggcgc
    25081 gggccttgga acgcgcctcc gtccgctaac cgatgatcta cccaagccgc tcgtgccggt
    25141 ttttcaaaaa ccgctcatca ctttcgcgct tgatcatttg atcgacacgg gcgtgaactc
    25201 tttcgtcatc aatacgcaca gacttccgga attgtttcgg gatttttttc gggagaacaa
    25261 ttacaccggt cgttcggtca cgcttgttca cgagccgcat ctactcgaca ccggcggcgg
    25321 catgaagaat atcgaacgct ttctcggaac acggcctttc gtcgtttaca gtggcgatat
    25381 tctaaccgac gcggacctgg aaccattgat cgaagagcac ttccgcgcca aaaatgatgt
    25441 tacgctgggc cttcggcgga ataccggcct cggcgcaggc gtggttatgc gcgatgggcg
    25501 aatcagcgcg atttcaaggg aatccaaccc aaaggaaaat tttgattatg caaacatatc
    25561 cgtgtggaat cccgaaattt tccacaggat tccggcaaag cggaagattt cattcattcc
    25621 aattctcgta gattggatcg gcgcaggcgg aaaaatcggg ggactggaac tcgatgatgg
    25681 gaagtggttc aacattggtt cacgaaagga atatctggac gtccaccgcg atatctcgac
    25741 cgaacgttgg aaaccgcgtt atgtgaccac gcgtgactgg tccgagcccg tcgccaaaag
    25801 cgcacaggtt gatgccaccg cggaaattcg cggatgttcg gttgtgggtg ccgactgccg
    25861 cgtggaagcc gatgtcattc tcgaggacac gattctgtgg ccgggtgcac aaattgcttc
    25921 acgaagcgag ctccaccgct gtattgtccg cacccaccgg aaagtgagcg gcactcatcg
    25981 caacctcgac atctagcctc gtgagaaccg atcttcttct gcgccgcacg cgcatgcatt
    26041 tcccgcggct cgataccgac aaagttaaaa ttcggccaat cgagaaaggc ggctccgacc
    26101 gcaagtttta ccgggtgcgt tgttcgccgg agcaaacact gattctggtg aagtacaatc
    26161 tcgagcgtga agagaaccgg cattacgtcg ccatcgcaca cttcctcgcc gagcacggca
    26221 ttcgcgcgcc gaaaatttac tatcacgatg cggaggaggg tttgatctgg ctcgaagatc
    26281 tcggcgagcg cgatctgttc agctatcggc atgagagctg gctcgtgcgg cgcgcgttct
    26341 acgaatcggc gctggatgag atcgcgaagc tgcatcggtt gccggaatct gtttgcctgg
    26401 cgatgcacca acatttgccg gccgaattta acgccgcgct ttatcggtgg gagcagaatt
    26461 atttcttcga aaactgcctt ggccgttttt acaagatcga caatgtggcg ctcgcgccgc
    26521 tcgccgcgtt gccggcactc aacgaaattg ccgagcggct cgacaaactg ccgcgcgttc
    26581 tcgtgcatcg ggattttcaa tcgcagaaca tcattgtgcg caacagccag acccatctga
    26641 tcgacttcca gggaatgcgg ccggggctgg cgcagtacga tctcgcctcg ttgctctacg
    26701 atccatatgt cgatcttagc aacgctgagc gcgaagaact gctcgatcac tactgcggcg
    26761 ggaagccgtc agcagagttc cgcgagattc tgcgcctttg cgcgatacag cgcctcatgc
    26821 aggcactcgg cgcctacggg ttcctcgggc tcgtcaaagg ccacaaacat ttcctcaaac
    26881 acattccccg cgcgatgcga tcagtttgcg aaatgatcag caacaccgcc ggtctggaaa
    26941 agcttgcggc ggtggtttgc gagctagctg acgaacgggt tcgttaggcg ctcggttccg
    27001 atttttgtcg gcggaccatg cccgggcagc acggtcacat tgtcgccgag cgggaaaagt
    27061 ttcgtccgaa taccggcgaa caaaagatcg acatctccac ccggcagatc ccagcggccg
    27121 acgccgccgg cgaatagaac atcgccaccg atgagcaatt cctcgttgcg cgagaaaaag
    27181 caaaggctgc cgggacaatg tcccggcact tccagcactt gcatcgtggc gccgaggaaa
    27241 tctcgcgccg gcgtttcgct gatgatgaag tcagcttcgg ccggttcgat ctccagctcg
    27301 aacccgaagt tgcggaagaa atcgcgatcg gaaatcatcg ttgcggtctc ggcgctacag
    27361 cccagcgggc aattgaaacg gcgtttgatt ttcgctacgc cctggacgtg atcgacgtgg
    27421 ccgtgcgtga ggagaagcaa cttaagatcg acatttcgtc gttcgagcca atcgcaggcg
    27481 ccgtccggcg catcgaacag catcaatcct tccggcgctt caaccagata gcagttcgtc
    27541 tcgaaaatcc cgccgcagaa cgtctcgtaa atcatctgaa aaccagttcg gcggtcgcaa
    27601 accggcgcta atttagactc tgggaacgaa agctgcatcc aggcggcgca gttttctcga
    27661 tttttgaatg tccatgcgcg cgggttgtct cattgtctac aggttaaggc cgccgtttgt
    27721 tgcctttccg ccataattct gccaatatgc gagctctttc ttttcgatga aacaacgctt
    27781 ctcccttctg ctggcgctcc cggcagtcgt tctcctcagc gcggcgatgc cacaaccgct
    27841 gcaggcgaaa tcggatgccg aacagatctg cgtctcggtc ggcagactgc tcgaagaagg
    27901 tcattacacg catcaaccgc tgaacgccgc cgtctcgcgc aaatttcttc agacctacct
    27961 cgagttgctc gattacagcc atcttttctt cacgcagaaa gacatcgacg cgctcaacac
    28021 caagtacggc gaggcgctcg gagacgacgt gctgctgggc aatctcaaac cggcttacga
    28081 gatcttcgat ctttatgcca aacgcgtgga tgaacgcgtc gccaagatca aagagttcct
    28141 caaaggaccg gtcgatttta aaaccgatgg gtcgatcgac ttgagccgac aaaaagcacc
    28201 atggccgaaa gatgaagccg aagcggacga gctctggcgg ggtcgcatta cgaacgaact
    28261 cttgcaggaa aaattgagcg aacatcctat cgagcctggg ccgcaactcg tggcgcgacg
    28321 ttatgatcgg ctcgcccgca ccgtgcacga ggaggacaga aacgaacagg tgaagctcta
    28381 cctcgacgcc cttgcccaga cctacgatcc gcattcggaa tatctgagca aagccgatct
    28441 gaaaaacttc agcatcaaca tgggcctctc gctcgtcggc atcggcgcga tgctgcgcag
    28501 cgaagacggt tacgccaaga tcgagagttt ggttccgggt ggcccggcgc aaaccgacgg
    28561 ccggctaaaa gtaggcgacc gcatcacagc ggtcgcgcag gggccggctg aatatgtcga
    28621 cgttcgagaa atgcgcctcg acaaagtggt cgagatgatc cgcggcaaaa aaggcacgca
    28681 cgtgcggctg cttgtcatcc cctccgacgc ggccgatcca tcgaagcgta agtcggtcga
    28741 gctcgtgcgt gatgagatca aactgaaaga ccaggaagca cgcgccgaca ttattattaa
    28801 gaaagacgag aacggcgacc cgataaaact cggttggctt acgctgccat cgttctacgc
    28861 cgatatggat cggcactcca agagcaccac ccgcgacgtg ctcgcgttgc tcaaacggct
    28921 gaagaaggaa aacatcgccg gcctcgttgt cgatcttcgc cgcaacggcg gcggttccct
    28981 cgaagaagcg atttcactca ccgggttgtt tttaaagtct ggtccagtcg tgcaaacgaa
    29041 ggaatcgaac ggctcaatcc gcgtctcgtc tgaccccgat ccggggatca tctattccgg
    29101 gccgcttgtc gtgttgacga gccgacagag cgcgtccgcc agcgagattt tcgccggtgc
    29161 gctgcaggat tatggccggg cggtcatcgt cggcgatcac aataccttcg gcaaaggcac
    29221 cgtacagaca attctcgagc tcggccgttt tacttcattg ctcggcagtc gctcgcaaga
    29281 agacggcgcg ctgaagttga ccatccagaa gttttatcgc gtcgccggtg gctcgactca
    29341 gctccgtggc gtcgaatcgg acatcgtgct gccgagcttg accgatttgc acgaattcgg
    29401 cgaaggcgcg ctcaagaatt gtttgcccta tgatgaagtt gctccggtcc gctacacgaa
    29461 atggtcggac acgcattcgc tctttatcga ccagttgaaa ggccgctcgg ccgagcgtgt
    29521 gcagaacatg actgagtttc attacgtcat ggaggacgtc gcgcggttac aacagaagat
    29581 caaagacaac cggatctcgt tgaacgaaga tgcgcggcgc aaggaactgg aggaggacaa
    29641 aattcgtaag gagacccgcg cgaaggagcg cttggcgcgc aacgatgaag agccgcgcat
    29701 ttatcggctt accctcgaca ccgtcgacaa accaaatctg cagctgatca tgtacccggg
    29761 gaaagtggcg gaggcgaaag cgaaaggcga gccgaccaaa gttgcgccgg aggctgcacc
    29821 agatgatgaa tccgaactaa tcggcgctga ggacgacacg aaagagccgg tgatcgaccc
    29881 ggagcgcgac gagtccctca atatcctggc cgatctcgtc gatctttccc gcgggccgaa
    29941 aaccgcgagc gcgaacagca gcaaatctcc cgcggcgcga ccgtaagatt aggcattgta
    30001 gccacggccc tgcgggccgt cagaagaaga taggccacag cccccgtggc tacagcatcc
    30061 cgtgtcaccg ccaccacggc tcgacgtggc ggggatccat tcccggcggg aatcggccat
    30121 cgtaaatcgg cggcatcggt tggccaaacc acggccgaaa cgtgcgctgc ggctgcggcc
    30181 cataccggaa gtaaccgttc gtgcgcggcg tgcggccgaa aaccgtcacc ggcgtgccga
    30241 catcgaccgc attataaaac gcgatcgcca ggtcctccgg catccgcaca caaccgtgtg
    30301 acgccggata acccggcaaa tacccggcat gcattccgtc ggcagcgtgg aaccgcatga
    30361 accatggcat cggcgcggga acaaacttgc agccgctcgg caccttcatg tccacatcgg
    30421 catcggctac caccgtgttg ccgtaggcgt cgacgatctt gccgtagatg ctggagtaat
    30481 gtttgcgctc tttctcgatg actgtgaacg agccggtctg cgtcagatga ccatagcgcc
    30541 cgctcgagat cggtgactct agcgcaacgt accgaccacg gagaagatac gccgtctgct
    30601 gcccgagatc gatttcaacc cggaacgggt ttacgtcttg cgccaagact gacgcggcga
    30661 ccgagcagaa ggtgaatcca gctaagccaa cagctatggc gcggcaggca attcccccga
    30721 gaaccctcac accttctttc aacccggctc tgcggaaaaa gtctcaggtg gatgcgcgtc
    30781 ctgcttctgg ggagcacagg ccgctggcct gttccattcg gctcccagcc caatgcaatt
    30841 tgcacatcac aagttcagca cgagaagcag cttcgcggca agctgcccga agtgcaggct
    30901 ggcagcctgc gctcgccaga acaaatcgcg acgcctcgat tgaagagtta ccggttatcg
    30961 actacctgcg gcgcaggtct cggcaattca actcgctcgc tcactttgcg aatcgcttcg
    31021 ctctgctcat gcagttgttg ctttagttcg gcgatttcct tttgctcctc ggttcttgct
    31081 gtttccagtt cctgcacttt gcggtgctct ttcaggaact cgttgagcag catcgcgttc
    31141 accgcctcat agcgcacagt atagactttc ccgtcggcgt cgcgtgtcac tagagcagcg
    31201 ttgacctttt ccacgtcctc ggcgacaagg ccaaactgcg ggatgccttc gggatcaatc
    31261 tccttgttgt aacggaaggt gactggttta ag
//
LOCUS       DASTDG010000018         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_953860, whole genome shotgun sequence.
ACCESSION   DASTDG010000018 DASTDG010000000
VERSION     DASTDG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953860"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFO30_00700"
     CDS             complement(<1..910)
                     /locus_tag="VFO30_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9417834.1"
                     /translation="MSQPELPALSIAELQMLLRRREISPREVIESLRARIEDVDAKLD
                     AYLSLDFDAAAQEAENADVDLPLGGVPIAIKDIINVAGQPCTCASKILRNYRAPYNAT
                     VIRRLRAAGAIPFGKTNMDEFAMGSSTENSSVKPTRNPWDLSRVPGGSSGGSAAAVAS
                     NAAFGALGTDTGGSIRQPAALCGIVGVKPSYGRVSRFGLVAFASSLDQVGPLTRTVRD
                     SALIMNAIAGHDPHDSTSLNQAVPDYTAALGRDLRGIRLGLPKEYMIEGIDPQVKSAI
                     DAALKQLQSLGADIVDVRLPHTDYGIA"
     gene            complement(907..1197)
                     /gene="gatC"
                     /locus_tag="VFO30_00705"
     CDS             complement(907..1197)
                     /gene="gatC"
                     /locus_tag="VFO30_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9417835.1"
                     /translation="MADTSELDVAYVARLARLNLSEEETQLFQKQLGDVLKYAAKLAE
                     VDVSHVEAAAHAIPVFNVFREDEPCDWFSPEEALSNAPRAANQLFIVTKVVE"
     gene            complement(1212..1574)
                     /locus_tag="VFO30_00710"
     CDS             complement(1212..1574)
                     /locus_tag="VFO30_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417836.1"
                     /translation="MLRRSQKSATPLHRQREELAQRELELRDRVAKLERMIAAAPKLA
                     EEAERRQREELLLRAQGGASRLNVSPGLQDKRYGEAASMRPRGALRKAQREGRIIFLV
                     LAIALAALVIWLVSHLHF"
     gene            complement(1578..2222)
                     /locus_tag="VFO30_00715"
     CDS             complement(1578..2222)
                     /locus_tag="VFO30_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417837.1"
                     /translation="MKKFGMPEKVRPIEQMLELTEEESISPEHLDEQVQKAQDQLLQL
                     KRQQEQIEKQKRELEELSRRQEELERGRAEMTDKLTRSLVVLEREAYDSQKRVEQLRG
                     MRESFAQHLQLLEAIDPKSWNPADLHKELSRALSAVDDARAEFTQQRSRLQASGEGGG
                     DTPLPEAAPEIARFGGGGRSLVQWLQIGFALSLPLIVFGSIALLIFFWLATSTR"
     gene            complement(2339..3562)
                     /locus_tag="VFO30_00720"
     CDS             complement(2339..3562)
                     /locus_tag="VFO30_00720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9417838.1"
                     /translation="MAGLVVKPRARILHGHDWVYASEVLKLFGDPADGDVISLKDGKD
                     RLIGSAIYNSKSQIVARRFSRQRQDLDLDFFKRRIAQAADYRARRKIDNRLYRVVWSE
                     SDGLPGVILDRYGETFVLQTLTLAMDRRKELIVNAVVDLFGGGASVSLANHQKASGTL
                     APLSIVERNDAPVRRAEGLDVRSGVLHGNAPTEMTIELNGLKFDLDLLHGHKTGFYLD
                     QKANYATVAEYAAGRRVLDCFANEGAFALSCARAGGVDVIAVEENANAVAAGKRNAQR
                     NELKVRWMEEDVFRFLRGAEKAGAEYDLVILDPPSFTKTKSGLRDALRGYRELHLRAF
                     KLLSKDGLLATFSCSHHVSEAAFAQTIADGLVDARRSARRLRRFEQALDHPVLPTLPE
                     TEYFKGALLELMPGR"
     gene            3651..4541
                     /locus_tag="VFO30_00725"
     CDS             3651..4541
                     /locus_tag="VFO30_00725"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9417839.1"
                     /translation="MQTPPQSSVDLPGIKKLRSGKVREVFDLGETLLFVATDRISAFD
                     VVLPDPIPHKGAVLNQLSAFWFKRFDEIDNHFVTADFNQFPKELRDFREQLTGRSMIV
                     RKTKPLPVECVVRGYLAGSGWNEYQRSQGVCGIKLPAALKQASKLPEPIFTPATKAES
                     GHDENIDMQRCAEILGKQTADRVKELSLQIYSAGRDHADQRGIIVADTKFEFGMLGGK
                     LLLIDEVLTPDSSRFWPKDQYVIGQNPPSFDKQFVRDYLETLDWNKTPPGPKLPASVI
                     EKTAAKYREAFERLTGAPLS"
     gene            complement(4545..>4798)
                     /locus_tag="VFO30_00730"
     CDS             complement(4545..>4798)
                     /locus_tag="VFO30_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9417840.1"
                     /translation="IIDTVRSLTTDTAEGDWHSVAVCSDGSYGVEEGLICSFPVRSRG
                     GKWEIVKDVSLNEFSKAKFDASVAELKEEKSMVSELLGA"
BASE COUNT          969 a         1502 c         1315 g         1012 t
ORIGIN      
        1 cagcgatgcc gtagtctgtg tgcggcagcc taacatcgac aatgtccgcg ccgagcgatt
       61 gcaactgctt cagcgccgcg tcgattgccg atttcacctg cggatcgatt ccctcgatca
      121 tgtactcctt cggcaacccg agccgaatcc cgcgtaaatc gcgaccgagc gcggcggtgt
      181 aatccggcac agcttggttt aacgacgtcg agtcgtgcgg atcatgaccg gcgatcgcgt
      241 tcatgatcaa cgcggaatcg cgcactgtcc ttgtaagcgg gccgacttgg tctagcgacg
      301 acgcgaacgc gaccaggccg aagcgcgaga cacgaccgta actcggtttc actcctacga
      361 ttccgcaaag cgctgctggt tggcgaatgg aaccaccggt atcggtgccg agcgcgccga
      421 aggctgcgtt agaggcaaca gcagcagcgg agccgccgct cgagccaccg ggtacgcgcg
      481 acaagtccca cggatttcgc gtcggcttga cgcttgagtt ctccgtggac gagcccattg
      541 cgaactcgtc catgttcgtt ttgccgaagg gaatcgcgcc ggccgcgcgc agacgccgga
      601 tcaccgtcgc gttgtacggt gcgcgataat tgcgcaggat ttttgacgcg caggtgcagg
      661 gctgaccggc gacgttgatg atgtccttga tcgcaatcgg gacaccgccg agtggaagat
      721 cgacatctgc gttttctgct tcttgcgccg cggcgtcgaa atcaagcgag agatacgcat
      781 cgagttttgc atcgacgtct tcaatccgag cacgtagcga ctcgatcact tcccgcggcg
      841 aaatctcgcg gcgccgaagg agcatctgca gttcggcgat actgagcgcc ggcaattccg
      901 gctggctcat tcgacgactt tggtaacgat gaacagctgg ttcgccgcgc gcggcgcgtt
      961 gctcaatgct tcctcggggc tgaaccagtc gcacggctcg tcttcacgaa acacgttgaa
     1021 gaccggaatt gcgtgcgcgg cggcctcgac gtggctcaca tccacttccg ccaatttcgc
     1081 cgcatatttc aacacgtcgc cgagctgctt ctgaaacagc tgcgtttcct cctcgctgag
     1141 atttaaccgc gcaagtctcg cgacgtaagc gacgtccagt tcggatgtat cagccatgcg
     1201 attgtcgatc cttagaagtg caaatggctc accagccaga tcacgagcgc ggcgagcgca
     1261 atggcgagca caagaaaaat gattcgccct tcccgttgtg ctttgcgcag cgcgccgcgc
     1321 ggccgcatgc tcgccgcctc gccgtagcgt ttgtcctgca atccgggcga cacattgagc
     1381 cggctcgcgc ctccttgcgc gcgcagaagc agttcctccc gctgccgtcg ctccgcttct
     1441 tccgccagct ttggcgccgc agcaatcatc cgctcgagtt ttgccactcg atcgcgcagt
     1501 tccaattcgc gttgcgccag ttcttcgcgc tgtcggtgca acggcgtggc cgatttctgc
     1561 gagcgccgaa gcatgcgtta ccgcgtagac gtcgccaacc aaaagaagat cagcagcgcg
     1621 atagagccga aaacgatcag cggcaaactg agcgcaaacc cgatctgcag ccactgtacg
     1681 agggaacggc cgccgccgcc gaaccgcgca atttccggcg ccgcttccgg cagtggcgtg
     1741 tcgcctccgc cttcaccgct cgcctggaga cggctgcgtt gctgcgtgaa ttcggcgcgc
     1801 gcgtcgtcta cggcgctgag cgcgcggctc agttccttgt gcagatcggc cgggttccag
     1861 ctcttgggat cgatcgcctc gagcaattgc aagtgttgcg caaaactctc ccgcatgccg
     1921 cgcaactgct cgacccgttt ctgcgaatcg taggcttccc gctcgagcac tacgagcgag
     1981 cgcgtcagct tgtcggtcat ttccgcgcgg ccgcgttcca gttcctcctg acgccggctt
     2041 aattcttcca gctcgcgctt ttgtttttcg atctgttcct gctggcgctt gagctggagc
     2101 aattgatcct gcgctttttg cacctgctca tcgagatgtt ccggggagat cgattcctct
     2161 tcagttaatt ccagcatctg ttcgatcggt cggacttttt ctggcatccc aaacttcttc
     2221 acggcatctg cctccgcgcg cgtactcgag gccgacgcca cattaatgag ccgagttgat
     2281 aattccagat ttttcccgcg agaaaaattt ctcgcaccgt gattgtcgac gtcgcgcttt
     2341 accgaccggg catcagttcg aggagcgcac ctttgaaata ttccgtctcg ggcaacgtcg
     2401 gcagcaccgg atgatcgagc gcctgttcga atcggcgcaa tcttcgcgcc gagcggcgcg
     2461 catcgaccaa tccgtcggca atggtttgcg cgaaagcagc ttcgctcacg tgatgcgagc
     2521 atgaaaacgt tgcgagcaaa ccgtctttcg acaaaagctt gaacgcgcgc aggtggagct
     2581 cgcggtaacc gcgcaacgcg tcgcgcaaac cgcttttcgt cttcgtaaac gacggcgggt
     2641 cgagaatcac cagatcatat tccgcgccgg ccttttccgc gccgcgtaaa aaccgaaaaa
     2701 cgtcttcttc catccagcga accttcaatt catttcgctg cgcattccgt tttccagccg
     2761 cgacggcatt cgcattctcc tccactgcga taacatcgac cccaccggcg cgcgcgcagc
     2821 tcagcgcgaa agctccttca ttcgcaaaac agtcgagcac gcggcggccg gcagcgtact
     2881 cggcaacggt ggcgtaattt gctttctggt cgaggtaaaa accggtcttg tgtccatgca
     2941 aaaggtcgag atcgaacttg aggccgttga gttcgatcgt catttcggtc ggcgcgttcc
     3001 cgtgcaaaac cccgctgcgc acgtccaatc cctccgcgcg ccgaaccggt gcgtcgttcc
     3061 gctcgacgat tgaaagtggg gcaagcgtcc cgcttgcctt ctgatggttc gcaagcgaga
     3121 cgctcgcgcc accgccgaaa agatcgacaa ccgcattcac gatcaactct tttcgccggt
     3181 ccatcgctag cgtcagcgtc tgtaaaacaa aagtctcgcc gtagcgatcg aggatcacgc
     3241 ctggtaaccc gtcgctttcg ctccagacaa cccgatacag gcgattgtcg attttgcgcc
     3301 gcgcgcggta atcggccgct tgcgcaattc gccgtttgaa gaaatcgagg tcaaggtcct
     3361 ggcgttgccg cgagaaacgc cgcgcgacga tttgcgattt ggaattgtaa atcgcactgc
     3421 cgatcagccg atctttgccg tccttgagcg aaatcacgtc gccatcggcc ggatcaccga
     3481 aaagtttcag cacttcgctg gcgtaaaccc agtcgtggcc gtgcagaatt cgcgcgcgcg
     3541 gtttgacgac cagtccggcc atgcttgaag tgagaagtga gatgtgaatg gtgagaagta
     3601 gtcacccgaa cgacctctca cttctcacct ctcactaatc acgctcattt atgcaaacac
     3661 cgccccaatc ctcagtcgat cttcccggca tcaaaaaact gcggagcggc aaagtgcgcg
     3721 aagtcttcga tcttggcgag acgttgctct tcgtcgcgac cgaccgaatt tcggcgtttg
     3781 atgtcgtcct gccggatccg attccgcaca aaggcgcggt gttgaaccaa ctctccgcgt
     3841 tctggttcaa acgtttcgac gagatcgaca atcactttgt cactgctgac ttcaatcagt
     3901 tcccgaaaga gctgcgcgat tttcgcgaac aactcaccgg ccgctcgatg attgtccgca
     3961 aaacaaagcc gttgccggtg gaatgcgtcg tgcgcggtta tctggccggc tccggttgga
     4021 acgaatacca gcggtcgcaa ggcgtctgcg ggatcaaatt gccggctgcc ttaaaacagg
     4081 cgtcgaaatt gcctgagccg attttcacgc cggcgacgaa agccgagagc ggacacgacg
     4141 agaacatcga catgcagcgc tgcgccgaaa ttctgggcaa acaaacagcc gaccgcgtca
     4201 aagaattgag tctgcaaatt tactcggctg gacgggatca cgccgatcag cgcggcatca
     4261 tcgtggcgga cacaaagttc gaatttggaa tgctcggcgg gaaattgctg ttgatcgacg
     4321 aagttctcac tcccgactca tcacgcttct ggcccaaaga ccagtacgta atcggacaaa
     4381 acccgccgag cttcgacaaa caattcgtgc gcgattatct cgagacgctc gattggaaca
     4441 agacgccacc cgggccaaaa ctgccggcga gcgtcatcga gaaaaccgcc gcgaaatacc
     4501 gcgaagcatt cgaacgactg actggggctc cgctctcctg attgtcaggc gccgagcaat
     4561 tcgctcacca tcgacttctc ttccttcagc tcagcgaccg acgcgtcgaa cttcgctttg
     4621 ctgaattcgt tcagcgacac gtccttcacg atttcccatt ttccgccgcg cgatcggact
     4681 ggaaacgagc agatcaatcc ttcctcaacg ccgtaacttc cgtcggaaca aaccgccaca
     4741 ctgtgccagt cgccctccgc tgtgtcggtg gtcaacgacc ggaccgtgtc tataatcg
//
LOCUS       DASTDG010000019         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1590685, whole genome shotgun sequence.
ACCESSION   DASTDG010000019 DASTDG010000000
VERSION     DASTDG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590685"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFO30_00735"
     CDS             complement(<1..416)
                     /locus_tag="VFO30_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9417841.1"
                     /translation="MNSTEACIALNMLPTVGPVRLRKLLQVFKEPQQILAANRADLGK
                     VDGIGNEVADQISNWESIVDLAGELKRVRDCGATMITQESPDYPKPLREIHAPPIVLY
                     VWGELQERDNHAIGIIGARRTTHYGTESAKKLAYQLA"
     gene            complement(483..932)
                     /locus_tag="VFO30_00740"
     CDS             complement(483..932)
                     /locus_tag="VFO30_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9417842.1"
                     /translation="MASFNKVILLGNLTRDPEVRYTPKGSAVCDLAIAVNRQYTLESG
                     EKREEVTYVDVVLWSRLAEIAGEYLKKGRPVFIEGRLQMDTWDDKQTGQKRSKLRVIA
                     ETMQLLGGRPASGAAEAGGEERLARPSKTTPPPPSATAEPDDDEIPF"
     gene            complement(951..1289)
                     /locus_tag="VFO30_00745"
     CDS             complement(951..1289)
                     /locus_tag="VFO30_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9417843.1"
                     /translation="MKNRYEALLVLNTQGRDDNVKEIVARLESEFQNEGAEIEQVQKM
                     DKRQFSYAAGPLDSGYYVNFIFEADPQLITKLRTKFKLDPEVYRQHYQRLPEKQEKKE
                     ESAKKLARAK"
     gene            complement(1286..1852)
                     /gene="pth"
                     /locus_tag="VFO30_00750"
     CDS             complement(1286..1852)
                     /gene="pth"
                     /locus_tag="VFO30_00750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9417844.1"
                     /translation="MPVRLVAGLGNPGPEYARTRHNLGFAVVDLVASQLGAAWDKPSK
                     WEAAFAKTGDVLLAKPLSYMNRSGHPLFAIAKFYKIPAKEILVVLDDLDLPLGRLRIR
                     LSGGPGGHNGLESIIVQFGTEEIPRLRVGIGAPPGEGAVDYVLSPFLDEEKPIVRSAI
                     ERAGEAVKCAIDNGIVAAMNKFNKSEDQ"
     gene            complement(1937..2644)
                     /locus_tag="VFO30_00755"
     CDS             complement(1937..2644)
                     /locus_tag="VFO30_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9417845.1"
                     /translation="MAKQVKIKAEHRAGTGRSAVRKLKARGIIPAVIYGAKQKPEPLQ
                     VSARDITTILSHASGENILVELEIDGEKGGRAAMLQEIQHSPLGDEILHVDFHAVSMD
                     EMIEADVPLEAVGVPNGVKNFGGLLEQSLRSLTIECLPSDLPDRITVDVSALNIGDAI
                     HVRDIKLPRGVTAQVQPDLTAFSVLAPAVEEVPVAAPAEAAVAGPEVIKEKKEEGEAA
                     AAATPAKETAPKEKEKK"
     gene            complement(2628..>2825)
                     /locus_tag="VFO30_00760"
     CDS             complement(2628..>2825)
                     /locus_tag="VFO30_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417846.1"
                     /translation="IPRLQKSKIEELITTNSTPVRPVEGFKTTVLCVSELLGEGMMRI
                     HKDESVSSLFNIDNGPNGKTS"
BASE COUNT          533 a          868 c          769 g          655 t
ORIGIN      
        1 gctagttgat aggcgagctt ctttgctgat tcggtgccgt aatgagtagt gcgccgcgcg
       61 ccgatgatcc cgatggcgtg attgtcccgc tcctgcaatt cgccccaaac gtaaagcaca
      121 atcggcggcg cgtgaatctc gcgcagcggc ttcgggtagt ctggcgattc ttgcgtgatc
      181 atggtcgccc cgcagtcgcg cacccgcttc agctcgcccg caagatcgac aatcgattcc
      241 caattggaga tttgatccgc cacttcattt ccaatcccat cgaccttgcc caggtcggcc
      301 cggttcgccg ccagtatctg ttgcggctcc ttgaatactt gcagcaattt ccgcagccgc
      361 accggaccga ccgtcggcag catgttgagc gcgatgcacg cttcggtgga attcatcaaa
      421 aagtagcgca agcgtcccgc ttgcggacca tctaaaacgc aagccagagg cttgcgctac
      481 aatcaaaacg gaatctcgtc gtcgtccggc tcggcggttg cggaaggtgg cggaggcgtg
      541 gtcttgctgg ggcgcgccag ccgttcctcc ccgcctgctt cggcggctcc actggcgggt
      601 cgaccaccca gtaactgcat cgtctcggcg atgacgcgca gtttgctacg cttttggccg
      661 gtctgtttgt cgtcccaagt gtccatttgc aagcgacctt cgatgaaaac cgggcggcct
      721 ttcttcaaat actcgcccgc gatctcggcc aggcgcgacc ataggacgac atcgacataa
      781 gtcacctcct cgcgcttttc gccgctctcg agagtgtatt ggcgattaac cgcaatagcg
      841 aggtcgcaga ccgcgctgcc tttcggcgtg taacgcacct cggggtcacg cgtcagattg
      901 ccgaggagaa tgactttgtt aaagctggcc atgcgccgct tataacgaaa tcacttcgcg
      961 cgcgccaact ttttcgctga ctcctctttc ttctcctgct tctccggcag acgttggtaa
     1021 tgctgccgat aaacttccgg atcgagcttg aatttggttc ggagtttggt gatgagctgc
     1081 gggtcggcct cgaaaatgaa gttcacgtag tagccggaat cgagcggccc ggcggcatac
     1141 gaaaactggc gcttgtccat tttctgcacc tgctcgatct ccgcgccttc gttttggaat
     1201 tctgattcca gccgcgcgac gatctccttc acgttatcgt cgcggccctg cgtgttgagc
     1261 acgagcaaag cttcgtaacg attcttcatt ggtcttcaga cttgttgaac ttgttcatcg
     1321 ccgcaacgat tccattgtca atcgcgcatt tcaccgcctc gccggcgcgt tcgattgccg
     1381 atctcactat cggtttttct tcgtccagaa acgggctgag cacgtaatcg accgcgcctt
     1441 cgcccggcgg tgcgccgatc ccgacgcgca accgcggaat ttcttctgtc ccaaattgta
     1501 caatgatcga ctcgagcccg ttatggccgc ccggaccgcc gctcaatcga atccggagtc
     1561 gtcccagcgg cagatcgaga tcatcgagca ccacgagaat ttccttggct ggaatcttgt
     1621 aaaatttcgc aatcgcgaac agcgggtgac cgctgcggtt catgtaactc agcggcttag
     1681 ccagcaggac atcgccagtt ttcgcaaatg ccgcctccca tttgctcggt ttgtcccaag
     1741 ccgcgccaag ctgcgacgcg acaagatcca caacggcaaa tccgaggttg tgtcgcgtcc
     1801 gcgcgtactc cgggccggga ttaccaaggc cggcgacgag gcgaacaggc atggtggatc
     1861 ggcttctccg aagacgatat taaaaatgcg gctacgccgc cattttgtac caacgcgttc
     1921 ggagaacgcg ttccacctac ttcttctctt tttctttcgg cgcggtttcc ttcgccggag
     1981 ttgcagctgc cgcggcttcg ccctcttcct tcttctcctt gatcacttcg ggaccggcga
     2041 cggcagcttc agccggagcc gcgactggca cttcctcaac agctggcgcc aaaaccgaga
     2101 acgcggtgag atcaggctgc acttgcgcgg tgactccgcg cggcaacttg atgtcgcgga
     2161 cgtgaatggc gtccccgata ttcagcgcgg acacatcaac agtgatccgg tccggcaaat
     2221 cgcttggcaa acattcgatt gtgagcgagc gcaaactttg ctcgagcaag ccgccgaaat
     2281 tcttaacccc gttgggaacc ccgaccgctt ccagcggcac atcagcttca atcatctcgt
     2341 ccatcgagac agcatggaaa tcgacgtgca aaatctcatc gccgagcggc gagtgctgaa
     2401 tctcctggag catggcggcg cgaccgcctt tctcgccgtc gatctccagc tcaaccaaaa
     2461 tgttttcgcc cgaagcgtgg ctcaggatgg tagtgatgtc gcgcgctgaa acctggagcg
     2521 gctccggctt ctgcttcgcg ccgtaaatca ccgcgggaat aatgccgcgt gccttgagtt
     2581 tgcgcactgc ggaacggcca gtgcccgcgc gatgctcggc tttgatctta acttgttttg
     2641 ccattcggtc cgttgtcgat gttgaacagc gaggagacgg actcatcttt gtgaatccgc
     2701 atcatcccct caccgagtag ctcggagacg cagagcacgg tggttttaaa tccttccacc
     2761 gggcgcaccg gcgtgctgtt ggtcgtaatt aattcctcga ttttggattt ttgcaaccgc
     2821 gggat
//
LOCUS       DASTDG010000020         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1456569, whole genome shotgun sequence.
ACCESSION   DASTDG010000020 DASTDG010000000
VERSION     DASTDG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456569"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VFO30_00765"
     CDS             complement(<1..169)
                     /locus_tag="VFO30_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9417847.1"
                     /translation="MSRQYTLQRAARSTSIHIDYAAELNEQQLAAVTASPGPLLIIAG
                     AGSGKTRTVTYR"
     gene            complement(242..679)
                     /locus_tag="VFO30_00770"
     CDS             complement(242..679)
                     /locus_tag="VFO30_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9417848.1"
                     /translation="MFRRKIDMLEVLLVHPGGPFWKNKDEGAWSIPKGEAAPGEDLLS
                     RAQIEFEEELGIKPAGNWIPLGSIKQKGGKTVHAWAFEGDLPQPFELKCNTFEIEWPL
                     HSGKMREFPEVDRAEFFPEQTARCKINAAQAAFLDRLRAALAG"
     gene            678..1742
                     /gene="dmeF"
                     /locus_tag="VFO30_00775"
     CDS             678..1742
                     /gene="dmeF"
                     /locus_tag="VFO30_00775"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HET9417849.1"
                     /translation="MSKPALTRLRAWFLVGRFTARDCKGNRPFVYCVAVHSQDHSRWR
                     HSHDFAVDFSLAERRTRIVIAITAAMMLLEIVVGLASNSMALLADGWHMSTHVSAFLI
                     TAIAYYFSRRHAQDVRFTFGTGKIGVLSGFSSAVLLAVVALLMAAESVRRMFAPLAIQ
                     FNEAIAIASLGLLVNLACALLLRDHHHDHEAKNRHHHHDLNLRAAYLHVLADAFTSVL
                     AIVALSSGKFLGWGWLDPIVGLVGSGVVFSWSYALLRDTSGILLDCTPATSALPDAIR
                     RSIESDGDSLVTDLHVWQIGAGKFAAIVSVVAHEANSPEKYHERLRSFTELVHVSVET
                     HVCEEHEPAHRDTGLLQPQK"
     gene            1813..2340
                     /locus_tag="VFO30_00780"
     CDS             1813..2340
                     /locus_tag="VFO30_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET9417850.1"
                     /translation="MEEAEVPLESLHEHVQHSAEHSGERWVAWVAFSTAVFAVLAAIA
                     GLLSGKCANEAMLNQIAASDQWNYYQAKSIKASVLDAKLALLATPNESDRAKLQRYET
                     EQEEIKSDAEQKQGEAKAEFQRHEVYARSVTMFQIAIAVAAISALTKRRRFWIVSLLF
                     GVVGCVFLALGALQH"
     gene            2346..2849
                     /gene="ruvC"
                     /locus_tag="VFO30_00785"
     CDS             2346..2849
                     /gene="ruvC"
                     /locus_tag="VFO30_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9417851.1"
                     /translation="MRVLSIDASLRNTGVAIVETDNGTTRALYYGTIRNCDTLPSSAC
                     LVAIRERLAELIHEHAPDSCALEAVIYVQSYKTAILLGAARGAAILAAAENGLPIFEY
                     APTRIKQATVGRGGAGKHQVAFMVRALLGLTETPEADAADALAIGLTHLRAQETAGRG
                     LPAHKPI"
     gene            2846..3535
                     /gene="lipB"
                     /locus_tag="VFO30_00790"
     CDS             2846..3535
                     /gene="lipB"
                     /locus_tag="VFO30_00790"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9417852.1"
                     /translation="MRPAFHWRWLGRVEFSKALARQEEIVASKRDSRAAPDELLLLEH
                     DPVYTIGKTPDQSSLIDPAQLPHPLFTINRGGQATYHGPGQLVGYPIVDLRRCGQDLH
                     RYLRWIEDLLIKTLRLFNISARTRSGLTGVWVQDRKIASIGVGVRHWITMHGFALNVC
                     GDLAPFDRIVPCGIANVTMTSIERETGAVYSPEDVSRRVAAIAFESIANLTTDGSLIQ
                     ISLHPDNAFLI"
     gene            3538..>3740
                     /locus_tag="VFO30_00795"
     CDS             3538..>3740
                     /locus_tag="VFO30_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9417853.1"
                     /translation="MAIPLEDNFTDVIGKAQRGLRISDSELAQQAGLVPAQIREVREG
                     KIDNEALKKIAPVLRLDANALVEL"
BASE COUNT          740 a         1105 c         1075 g          820 t
ORIGIN      
        1 cccgatatgt caccgttcgc gtcttacctg agcccgcgcc ggcaatgatg agcagcggcc
       61 ccggtgatgc cgtcacggcc gcgagctgct gctcattcaa ctccgccgcg taatcgatat
      121 ggatcgatgt cgatcttgcc gcccgttgca gcgtgtattg acgagacata aatgccagtc
      181 cgccctcgaa ccgctatgct ccggttaccg cgcgaactgt tcaaatcgga aattctgccc
      241 gtcagccggc gagcgcagcg cgcaagcgat caagaaacgc agcctgcgca gcgttgattt
      301 tgcagcgggc ggtttgttcc gggaaaaatt cggcgcgatc gacttccgga aattctcgca
      361 ttttgccgga gtgaaggggc cattcgattt cgaatgtgtt gcactttagt tcaaacggct
      421 gcggcagatc gccttcgaac gcccaggcgt gaacggtctt gccgcctttt tgtttgattg
      481 agccgagcgg aatccaattt ccggcaggct tgatcccaag ttcctcttca aattcgattt
      541 gcgcgcggct gagcaaatct tctcctggcg cagcctcgcc tttgggaatg ctccaggcgc
      601 cttcatcctt gtttttccaa aagggcccac cgggatggac cagcagcacc tccaacatgt
      661 cgatcttgcg ccgaaacatg agcaaaccgg cgctgacacg gttgcgtgcc tggtttctgg
      721 ttggtcgttt caccgcgcgc gattgcaaag gcaatcgtcc gtttgtttat tgcgtcgccg
      781 tgcattcaca agatcattcg cgctggcgcc actcgcacga tttcgcggtt gattttagtc
      841 ttgcggaacg gcgcacgcgc atcgtcatcg ccatcaccgc agcgatgatg ctgctcgaga
      901 ttgtcgtcgg tctggcgtcg aattcgatgg cgttgctcgc cgatggctgg cacatgagca
      961 cccatgtaag cgcgttcctc attaccgcga ttgcttatta ttttagccgc cgccacgccc
     1021 aagatgtccg gttcactttt ggcaccggaa aaattggggt gctcagcggc ttctccagcg
     1081 cggtgttgct cgcggtggtc gcgcttttaa tggcggccga atcggtgcgg cgcatgttcg
     1141 ctccgctagc tattcaattt aatgaagcga tcgcgatcgc ttccctcggt ctattggtaa
     1201 atctcgcttg cgcgctattg ctaagagatc accatcatga ccacgaagcc aaaaatcgac
     1261 atcatcacca cgacctgaac ctgcgcgcgg cttatctgca tgtgctggct gacgcgttta
     1321 ccagtgtgct cgccattgtc gccttgagta gcggcaaatt tttgggttgg ggctggctcg
     1381 atccgatcgt cggactggtc ggcagcggcg tcgttttctc ctggtcatac gccctgctgc
     1441 gcgatacgag cggtattctg ctcgattgca ccccagccac atcggcgctg ccggacgcga
     1501 ttcggcgcag catcgaaagc gacggcgact cgctcgtgac tgatctgcac gtctggcaaa
     1561 tcggcgcggg caagttcgcg gccattgttt ccgttgtcgc gcatgaagcg aactcgccgg
     1621 agaagtatca tgagcgcttg cgcagtttca ccgagctcgt gcacgtcagt gtcgaaacac
     1681 acgtatgcga agagcacgag ccggcacatc gcgacaccgg cttacttcag ccacaaaaat
     1741 gaatttcacg gctgcgcgac ttgcttgtcg cgcagggttt gagcggcgat tgtcgatgtt
     1801 gaattttcga tcatggaaga agccgaggtg cccttggaaa gcttgcacga acatgtgcag
     1861 cacagcgcgg agcacagcgg cgagcgatgg gttgcctggg tcgcatttag taccgccgtc
     1921 ttcgccgtat tggcagcaat cgccggcctt ctctctggaa agtgcgcgaa cgaggcgatg
     1981 ctgaatcaga tcgcggcctc cgatcaatgg aactattacc aggcgaaaag catcaaagct
     2041 tccgtgctcg acgccaagct ggctctgctc gccacgccta acgaatcaga ccgcgccaaa
     2101 cttcagcgct acgagaccga gcaggaggaa atcaagtccg acgcggaaca aaaacaaggt
     2161 gaggccaaag ccgagtttca gcgccacgaa gtgtatgcgc gcagtgtgac catgtttcaa
     2221 attgccatcg ctgtcgccgc gatttccgcg ctgaccaagc gccgtcggtt ctggatcgtc
     2281 agcctgctct tcggagttgt cggttgcgtt tttctcgcgc tcggcgcatt gcagcactga
     2341 attcgatgcg tgttctttcg attgacgcct cgttgcggaa caccggcgta gcaatcgtcg
     2401 agaccgacaa cggcacgacg cgcgcgcttt actacggcac gattcgcaac tgcgatacgc
     2461 ttccttcttc agcctgtctg gttgcgattc gcgaacggct cgccgaattg attcacgaac
     2521 atgcgcctga ctcgtgcgcg ctcgaggcgg tcatttacgt ccagagctac aaaacagcca
     2581 tcctcctcgg cgccgcgcgc ggcgcggcca ttctcgccgc ggcggaaaac ggcttgccga
     2641 ttttcgagta cgccccgacc cgcattaaac aagcgacggt cggtcggggc ggggcgggaa
     2701 aacaccaggt cgcgttcatg gtgcgcgctc tgctcggctt aaccgaaacg ccggaggcgg
     2761 atgccgctga cgcgctggcg atcgggctga cgcatttacg cgcgcaagag acggccggtc
     2821 gcggattgcc ggcgcacaaa ccaatatgag accggccttt cactggcgct ggctcgggcg
     2881 cgtcgaattt tcgaaagcgc tcgcacggca ggaggaaatc gtcgcaagca aacgagacag
     2941 tcgcgccgcc ccggatgaat tgctgctcct cgagcacgac cccgtttaca cgattggaaa
     3001 aacgcccgat caatccagtc tcatcgatcc ggcgcaattg ccgcatccgc tgttcacgat
     3061 caatcgcggc gggcaggcaa cctatcacgg ccccggtcaa ttggtcggct acccaattgt
     3121 cgatcttcga cgatgcgggc aggatctaca tcgctacctg cgctggattg aagatttgct
     3181 gatcaaaacg ctgcggctct ttaacatttc ggcgcgcacc cgctcgggct tgaccggcgt
     3241 gtgggtacaa gatcgcaaga tcgcttccat tggagtgggc gttcgacatt ggatcacgat
     3301 gcacggtttt gccctcaacg tatgtggcga tctcgcgccg ttcgatcgca ttgtcccatg
     3361 cggtatcgcc aacgtgacga tgacctcgat tgaaagagaa accggcgccg tttattcgcc
     3421 ggaggatgtg agccggcgcg tcgcggcgat tgcgttcgag tctatcgcga atctgactac
     3481 tgacggttca ctcatccaaa tctctctcca cccggacaac gcatttttga tttagcgatg
     3541 gcgattccgc tcgaagacaa tttcaccgat gtgatcggaa aggcgcagcg cgggttacgc
     3601 atttcggaca gcgaactggc gcaacaggcc gggcttgtcc cggcgcaaat tcgcgaagtg
     3661 cgggaaggca agatcgacaa tgaagcgctg aaaaagatcg cgccggtttt gcggctcgac
     3721 gccaacgcgc tcgtcgagct
//
LOCUS       DASTDG010000021         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1405571, whole genome shotgun sequence.
ACCESSION   DASTDG010000021 DASTDG010000000
VERSION     DASTDG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405571"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..2120
                     /locus_tag="VFO30_00800"
     CDS             219..2120
                     /locus_tag="VFO30_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417854.1"
                     /translation="MNLERFFSELKRRNVYKVAVAYAVVAWLLIQGGSILFPTFGAPA
                     WVMQVLVTVLVLGFPSALVFTWATASPPPEQPAPSPVIIEKSIAVLPFDNLSEEQANA
                     FFADGVHDEILSDLAKIADLKVISRTSVMQYRTGNRNLREIAKELGVAHILEGSVQRA
                     GNRVRLRAQLIDARTDAHLWAEKYDRPLDDVFAIQSEIAKAVAAQLQAKLSPAEKAAI
                     EQPPTADLIAYDRYLRSKKLLDQTSYDARTIDNTRQAIWLLEQAVAHDPKFYLAHCQL
                     AWTHDALYFIGADHSPARVAQARDALKRALTLAPERGEAHLAAAWISYHSRDYEGALA
                     ELGTARRTLPNDSSIPIVAGFIARRQNQWQQCTENLERAAELDPQRVWLLQQISQTYG
                     LLRRYADMARALDRAVAASPHDANTRVARALVDLEWHAHTQPAREEIYSLLSEDPSAV
                     DAVAEQWFTVALCRRDSTDIDRALASIPPEGIIPINVRMPRSFCEGVAARTRRDAAAA
                     ESAFTSARAEMLKLLDGQPDYAEALSVLGMSEAALGRKEDALQHSRRAIELLPITKDA
                     VTGAEILRNLAITCAWADEKDLAIKQLEEALQLPSPISYGQLRLHPWWDPLRGDARFD
                     KIVASLAPK"
BASE COUNT          484 a          662 c          666 g          459 t
ORIGIN      
        1 cttcgaaatg atcatggccg cggaagttaa gaaagaggtg cgaaaaatcg cgacaatcga
       61 aggtgaacga tcgacataca accaaagcga ggccactggc ttcattcgcc tcaatgcgct
      121 gcgactgaaa ctccgcgcag cgctgaaggc aagcagcgct tgagtgtccg cttcccaaaa
      181 atcgaaggtt ctagcaaaat cgggtcgagg ttagttgctt gaatctggag cgttttttct
      241 ctgagctgaa gcgtcgcaac gtttacaaag ttgcggtcgc ctacgcggtc gtggcgtggc
      301 tgctgatcca gggaggatca attttgtttc ccactttcgg agcgcccgcg tgggtcatgc
      361 aggtcttggt gacggtgctc gtgttgggct ttcctagtgc gctcgttttt acttgggcca
      421 cggcgtcgcc gccgccggag cagcccgcac cttcaccggt gattatcgaa aaatcgatcg
      481 cggtgcttcc ttttgacaac ctgagcgaag agcaagcgaa cgccttcttc gccgacggcg
      541 tgcacgacga aattctgagc gaccttgcga aaatcgctga tctcaaagtg atcagccgca
      601 cctcagtgat gcaataccgg acgggcaacc gcaatctgcg cgagatcgcg aaggagttag
      661 gcgtcgcaca tattctcgag ggaagcgtgc aacgtgctgg aaaccgcgtc cgcttgcgcg
      721 cccagctgat tgatgcgcgc accgacgctc atctgtgggc ggaaaaatac gaccgtccgc
      781 tggacgatgt cttcgccatc cagagtgaga tcgccaaggc ggtggccgcg caattgcaag
      841 caaagctctc ccccgccgag aaagcggcga ttgagcagcc acctactgct gacctgatag
      901 cttatgaccg atatcttcgg agcaagaagc tactggatca gacaagttac gacgcgcgca
      961 cgatcgacaa cacgcgccag gcaatctggt tgctcgaaca agctgtggcg cacgacccca
     1021 agttttatct ggcgcactgt caactcgctt ggacgcacga tgccttgtac ttcattggcg
     1081 ctgatcacag tccggcgcgc gtcgctcagg cgagggacgc gctaaagaga gctctgacgc
     1141 tcgcgcctga acgcggcgaa gcgcatctgg ctgcggcatg gatctcttat cattcccgcg
     1201 attatgaagg cgcactcgcc gaactcggga ccgcccgtcg gacgttgccg aacgattcgt
     1261 ctattccgat agtggctggc ttcatcgcac gccgccaaaa tcaatggcag caatgcacag
     1321 aaaacctcga gcgtgccgcc gagctcgacc cgcaacgggt ctggctgctc caacaaattt
     1381 cccagactta cgggctgtta cgccgctacg ccgatatggc gagagcgctc gaccgagcgg
     1441 ttgcagcttc gcctcacgac gcaaacacgc gtgttgcacg agcgctggtc gatctggaat
     1501 ggcacgccca cacgcagccg gctcgggagg aaatttactc tttgctgagc gaagatccga
     1561 gcgccgttga tgccgttgcc gagcagtggt tcactgtggc actttgccgg cgcgattcca
     1621 ctgacattga ccgcgccctc gcttccatac cgcccgaggg catcattcca atcaacgtgc
     1681 gtatgccgcg atctttctgt gaaggagtcg cggcacggac acgccgcgat gccgctgctg
     1741 cggaatcggc atttacatcc gcgcgggccg agatgttgaa actgctcgat ggacaacccg
     1801 attacgctga agccttgagc gtgttgggta tgagcgaagc ggctttgggg cgcaaagaag
     1861 acgctctgca acacagtcgg cgcgcgatcg agcttttgcc gatcaccaaa gatgcggtga
     1921 ctggcgcgga gatactcagg aatctggcga tcacctgcgc gtgggccgac gagaaggatt
     1981 tggcgatcaa acaactggag gaagcgctac aactacccag cccgatttcg tatggccaat
     2041 tgcgcttgca tccttggtgg gaccccctcc gcggcgacgc gcgcttcgac aaaatcgtcg
     2101 cctctctcgc gccgaagtag cgaattacct cgcagaattt gttcggcgag ctgacgcggc
     2161 gcaactttta caaagttgcc attccgtacg cagcgatcgg gtggctgctc gttcaggtcg
     2221 cgacgcaggt ctttcctttc tgcgaaactg caaactgggc gggacggttg g
//
LOCUS       DASTDG010000022         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_718700, whole genome shotgun sequence.
ACCESSION   DASTDG010000022 DASTDG010000000
VERSION     DASTDG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718700"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFO30_00805"
     CDS             <1..525
                     /locus_tag="VFO30_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417855.1"
                     /translation="IAIAALDALTVAGVGDASPRSTTAATTKIARVAIWDFDAHHGNG
                     TEAIVAHNSRVAFASIHQFPAYPGTGARSFANIDNYPVAPFTPRAEHVDVAKRALDKL
                     LAFEPTLLLISAGFDAYARDPLVQMTLERDDFAAFGEWLRKIDIPIAALLEGGYSDDL
                     PELIDAFLTAWDAS"
     gene            543..761
                     /locus_tag="VFO30_00810"
     CDS             543..761
                     /locus_tag="VFO30_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417856.1"
                     /translation="MRVLRADQFKQLHGGMFNLDMPVLTRPAFDREQRAPVDVAEIAI
                     GKFVADFCMFVVSFIDTEVPLPVFTKSM"
     gene            1089..1162
                     /locus_tag="VFO30_00815"
     tRNA            1089..1162
                     /locus_tag="VFO30_00815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1123..1125,aa:Pro,seq:ggg)
     gene            1538..2494
                     /locus_tag="VFO30_00820"
     CDS             1538..2494
                     /locus_tag="VFO30_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9417857.1"
                     /translation="MAAPKRFRVLVAFAALYLIWGSTYLGILFAIQSIPPFLMAGTRF
                     FLAGLIMFGIARTQGPLRSTWAEWRASLIVGACLLLCGNGGVTISEKYIASGLASLIV
                     ATVPIYIALLSWLSGMAPRPKPIVWAGLTGGFAGVALLLGPALRLSGVNSSRAATGMS
                     ILLVSSFIWSAGSLYSRSAKYPTSPFVAAAQQMFCGGLLLMLVGIAIGEPQRFNPGQI
                     SILSIGAFVYLVLIGAVVGYTAYFWLLRHCDPAKVSTYAYVNPIVAVLLGALFAGEKL
                     TLQTLLAAALIIGSVALIITVQQLRPQAAPPIATAVEVECAR"
     gene            2491..3672
                     /locus_tag="VFO30_00825"
     CDS             2491..3672
                     /locus_tag="VFO30_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET9417858.1"
                     /translation="MKTETQTDGAQDRQETINVVKRNWRAEVETAQVYRELATREPDE
                     KRKGILLRMAEAEERHAQRWAKKLVDLGEPLPALPDTFGRRLKRWLNRALGTEIAIRR
                     MEAAEEKHEAAFRDQRDRALAGEHDVKDFLRESALEEKAHARALQTMVPQLGPRTVLD
                     TILKRERWHGRGGSWVADAIYGVNDGLGAVFGIVSGVAGATNNQQHYILISGLAGMIA
                     SSLSMGAGAYLAVKSEAEVYEAEIAREKAEVEENPEEEIEEMSLFYQLQGFSPEEAQR
                     MAERLSENPDQLVRAMAQTELGLSEHRFPSPWKSSASAAISTAIGAFIPIIPFFFMSG
                     IAAVVWAFAISIVAHFAVGAVKSLITLRSWWASGLEMTIVGVIEAAVTYGLGLAFGAI
                     S"
     gene            3729..4088
                     /locus_tag="VFO30_00830"
     CDS             3729..4088
                     /locus_tag="VFO30_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417859.1"
                     /translation="MKIYFQVLVIAAGFCFAQTSFAQDPVKQSPDKYKVIFNNDRVRV
                     LDVHLKPGDKSPMHSHPNYLVYALSNSTVKFTFPNGKTSEFKTRTGQCVWRDAEKHAV
                     ENTGKTEAHVLNIELKH"
     gene            4525..>6206
                     /locus_tag="VFO30_00835"
     CDS             4525..>6206
                     /locus_tag="VFO30_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HET9417860.1"
                     /translation="MAVLTAIGSTALAGSATWAANPSTTDWNTAINWEPQTVPNGPLD
                     VATFATSSETAVSLSAQTEVSGITFNSGASAFTITVSANDNNFGLNLSGVGITNNSGT
                     TQKIATAYPGYIQFFNSATAGTSTSYVLNGGSMWFWNTSAAGSGTFTGYPWFHIYFQD
                     SATAQNGNFILNGPASIYFDNTSNAGSAAFTLNIDPITGEPSFMHFRDSATAQNGNFT
                     LYSQAQIYFFDTSSAGSGVFTLNGDPATGRNSVIYFQDSATAQNGNFTLNASAEIYFY
                     SHSSAGNGTFVVKGPGAGLYFTRYSKGGTSRVELFGTGTGDSADVFLDISGRNAPGLS
                     IGSLEGAGNVFLGSNRLTVGSNNLSTTFSGVIADYPGDPIGYSGSLSKTGKGKLTLSN
                     TNLYDGGTTIDNGTLVVKNGSGSATGSGPVQVNSGTLSGKGSISGAVTVGNGTTAGAI
                     LHPGIGAKPSRLLINNTVSFNATSAFKCYVKRSTTPVAGKLSALGVTIGTNVSFTLLE
                     TGTGTLTAGTVFTVIKNTSANPIFGRFSNLPDGGTITTVNGTVYKANYSGGTG"
BASE COUNT         1306 a         1805 c         1767 g         1328 t
ORIGIN      
        1 atcgccatcg ccgcgctgga cgcgctcact gtagccggtg tcggtgacgc cagcccgcgg
       61 tcgacgaccg cggctacaac gaaaattgct cgcgtggcga tctgggattt cgatgcgcat
      121 cacggcaatg gaacggaagc gatcgtcgcg cacaattctc gcgttgcctt cgcctcaatt
      181 caccagtttc cggcatatcc cggcaccggc gcgagatcgt tcgcgaacat cgacaactat
      241 ccggtcgcgc cattcactcc gcgcgccgaa catgtcgatg ttgcaaaacg cgcgctggac
      301 aagctactcg cgtttgagcc gactctcctg ctcatctcgg ccggctttga cgcctatgcg
      361 cgcgatccgc tcgtgcaaat gacactcgag cgcgacgatt tcgcggcctt cggcgaatgg
      421 ctgcgcaaga tcgacattcc gatcgccgcg ttactcgaag gtggctacag cgatgatctt
      481 cccgaattaa tcgacgcgtt tttaaccgcg tgggacgcat catagctggt ctttgattca
      541 acttgcgcgt tttgcgggcg gatcagttca agcagttgca tggcggcatg ttcaatctcg
      601 acatgcccgt actcacgcgc ccggcttttg accgcgagca gcgcgcaccg gtggacgtag
      661 ctgaaatcgc catagggaaa tttgtagcgg acttttgcat gttcgtcgtg tcgttcatcg
      721 ataccgaggt gccattgccc gtattcacta aatccatgta accgaatgaa ttcagtcttt
      781 gcggcggcag atggttgatg agcgcgccac gcacctttgc cgtcaacagt tacttgaccc
      841 gctctgatca actctcgtgc gtgctcgtac gccgcggtgt tcagctttac ggcatgagct
      901 gcaatatcgt ccgcgcctaa agctcgcgag gccgcgccgc aaatcagcca catggttgtt
      961 agcaattcag gcgctgttaa ccgcaatttc gtcattgccg acgcctactg atgtgaccgg
     1021 tgtgtgagga aagcgaccct gaaacgctag cgaatttctc atccctggtc gatagttcct
     1081 gctcgattcg ggccgtagca cagcttggta gtgcgcttga atggggttca agaggtcgcg
     1141 ggttcaaatc ccgccggccc gagctgctca attagtaaag aagatgtttt atctctacgt
     1201 cctttggagc agcaagacag gccgtcgcta tgtcgattcc tgctaggatg ttgaccaccg
     1261 tctgcgccgg cataacgccg gccaaagcaa cacagcaccg aattccatgg cggtgttaca
     1321 ttgcgaaagc ttcgcgaccc gcgccgacgc ggcgacaaag gagcgatact acaaaaccgg
     1381 tcacggtcgc gatgagttgg cgaagctgaa tccggagtgc ggtcgccgcg gcgacaggtc
     1441 gcgggttcaa atcccgccag cccgacaaga tcgcgcgttc gttgcgtcac gacatggagt
     1501 gtccttgctt cgcgagttga atcgcacatc gtgcaggatg gctgcgccga agcgatttcg
     1561 cgtgcttgtt gcgtttgccg cgctctatct catctggggc tcgacctatc tcggaatact
     1621 cttcgcgatc caatcgatcc cgccgtttct catggcaggc acgcgattct ttctcgcggg
     1681 attaatcatg ttcggaatcg cgcgcacgca agggccgtta agatcaacat gggcggagtg
     1741 gcgcgcgtca ctgatcgtcg gcgcatgcct gcttctctgc ggcaacggcg gcgtcacgat
     1801 ctcggagaaa tacatcgcct ccggtttggc gagcctgatt gtggcgacgg tcccgattta
     1861 cattgcgctg cttagttggc tcagcggaat ggcgccgcgt ccgaaaccga ttgtgtgggc
     1921 tggactgacc ggtggctttg cgggtgtcgc gctcttgctc gggccggcat tgcgattgtc
     1981 cggtgtgaac agcagccgcg cggccactgg catgtcgatc ttgctcgtct catcgttcat
     2041 ctggtcggct ggttcgcttt actcgcgcag cgcgaaatat cccacgtcgc cctttgtcgc
     2101 cgcggcccag caaatgtttt gcggcggttt gttgttgatg ctggtcggca ttgcgatcgg
     2161 cgagccgcag cggtttaatc ccggccagat atcgatcttg tcgatcggtg cattcgtgta
     2221 tctggtgctc atcggcgcgg ttgtgggtta caccgcttac ttctggttgt tgcgtcactg
     2281 cgatccggca aaggtctcca cttacgctta tgtcaatccg atcgtcgcgg tgctgctcgg
     2341 cgcgttgttc gcgggcgaaa aattgactct gcagacctta ctcgccgccg cattgattat
     2401 cggctcggtg gcgctgatca tcactgtgca gcaactgcga ccacaagcag cgccgcccat
     2461 cgcgacggcg gtcgaagtgg aatgcgcgag atgaaaacgg aaacgcaaac tgacggcgcg
     2521 caagatcgac aagagacgat caacgtagtc aaacgcaact ggcgcgccga ggtggagacg
     2581 gcgcaggtct accgcgaact cgccacgcgc gagccggacg agaagcgcaa aggcattttg
     2641 cttcgcatgg ccgaagcgga agagcgccac gcgcagcgct gggcgaagaa acttgtcgat
     2701 ctgggcgagc cgctgccggc gctgccggac acgtttggcc gtcgattgaa acgttggctg
     2761 aatcgcgcgc tcggtaccga aatcgcaatc cgccgcatgg aagcagccga ggaaaaacat
     2821 gaagcggcat ttcgcgacca gcgcgaccgc gcgctcgccg gcgagcacga cgtcaaagat
     2881 tttcttcggg aaagtgcgct ggaagaaaaa gcgcatgcac gcgcgttgca aacgatggtg
     2941 ccgcagctcg gaccgcgcac cgtgcttgat acaattctga aacgcgaacg ttggcacggc
     3001 cgcggcggca gttgggtcgc cgatgcaatt tacggcgtga acgatggtct gggtgcggtc
     3061 tttggaattg tctccggcgt cgccggcgcc acgaacaacc agcagcatta tattctcatt
     3121 tccggtttgg ccggaatgat cgcctcatcg ctttccatgg gcgcgggcgc gtatctcgcg
     3181 gtgaaaagcg aagcagaagt ttacgaggcc gagatcgcgc gtgaaaaggc cgaggtggaa
     3241 gaaaatcccg aagaagaaat cgaagagatg tcgctctttt atcaactgca gggattcagc
     3301 ccggaagaag cgcagcggat ggcagaacgg ttgagcgaaa atccggatca gctcgtgcga
     3361 gcgatggccc aaaccgagct cggtctctcg gagcatcgct tcccctcgcc ctggaaatcg
     3421 tccgcgtccg ctgcgatttc cactgccatc ggcgccttca tcccaatcat tccgtttttc
     3481 ttcatgagcg gaatcgccgc ggtcgtttgg gcattcgcga tcagcattgt tgcgcatttc
     3541 gccgttggcg cggtgaaatc attgatcacg ctgcgctcgt ggtgggcgtc cggcttggag
     3601 atgaccatcg tcggagtgat cgaggccgcc gtcacttacg gcctgggtct cgcctttggc
     3661 gcaatttcgt aaccgcgccg aacgaattgc gcttgcaaga ttgatatcga ttgggccaat
     3721 ttttgaccat gaaaatctat ttccaagtct tggtcatcgc cgccggattc tgctttgcgc
     3781 aaacgagctt tgcccaagac ccggttaagc aatcgcccga caaatacaaa gtcatattca
     3841 acaacgacag agtgcgcgtg ctcgacgtgc atctgaaacc gggtgataaa tcgccgatgc
     3901 attcgcatcc gaactacctc gtctacgcgc tcagcaattc cacggtgaaa tttactttcc
     3961 ctaatgggaa aaccagcgag ttcaagacga ggaccggtca atgcgtctgg cgggacgcgg
     4021 agaagcatgc ggtggagaac accggcaaaa ccgaagcgca cgtcctcaac atcgagctga
     4081 agcactaaac cacgcacaag atcgaccatc gcgcactgcg atcatcccta cttcccgcag
     4141 gagttcctcg aaatcatcga agtgatcgaa cggcttcaac cctgcatcgg caaaaagtca
     4201 atgcatcgga cgccattctg gaaatgtata ctcgcagttg cgccgagccg ccggcgcgcg
     4261 agtttgcgat caagaactcc gcagtcgctt tgtaggcgcg caccctctcg ggattctgaa
     4321 gttttgacgt cctacgtcat agcacgagcc ggtcgggttg tttagacgct agcgatttac
     4381 tccggccgtt gcggtacgct cagtgcttta gtgaaatgaa cacagaaggt gacgtccaaa
     4441 ttacattcgg gcgacctcat tgggtcgcct tatgctggac tgcgtcgcgc gaggcggttt
     4501 ctgccgcccc tgcttcacat cgtgttggcg gtcttaaccg cgattggaag caccgcgctt
     4561 gctggcagcg ccacatgggc agcaaatcca agcacgaccg actggaacac ggctatcaac
     4621 tgggaaccgc agacggtacc caatggccct ttggatgtcg cgacgttcgc cacctcgagt
     4681 gagaccgcag tttcactgtc ggctcaaacc gaagtaagtg ggatcacgtt caactccggg
     4741 gcgagcgcct tcaccatcac agtatcggcg aatgacaaca acttcggctt gaatctcagt
     4801 ggcgtgggca tcacgaacaa ttcaggcacg acacagaaga ttgcgactgc ctatcctgga
     4861 tacattcaat tcttcaacag tgccacggcc ggtacttcaa ccagttatgt gctcaacggg
     4921 ggttccatgt ggttctggaa tacctcggct gcgggcagcg gcacgttcac cggctatccc
     4981 tggtttcaca tttatttcca agacagtgcg acggcgcaaa atggcaactt catcctcaat
     5041 gggcccgctt ccatctactt cgacaacacc tcgaatgccg gcagcgccgc gttcacgctc
     5101 aacatcgatc caatcaccgg cgagccgtct ttcatgcatt tccgcgacag tgcgacggcg
     5161 caaaatggga atttcaccct ctattcgcag gctcagatct acttcttcga cacctcgagt
     5221 gccggcagcg gcgtgttcac cctgaatggt gatccggcga ctggccgcaa ctcggtcatt
     5281 tatttccaag acagtgcgac cgcgcaaaat ggcaacttca ccctcaatgc atccgctgag
     5341 atctacttct acagccactc aagcgcgggc aacggcacat tcgtcgtcaa gggccccggc
     5401 gcggggcttt acttcactcg ctattctaag ggcggcacct cgcgcgtgga gctgttcggc
     5461 acgggaacgg gcgattctgc ggatgtcttc ttggatatca gcggtcgcaa tgcgcccggc
     5521 ctcagcattg gctcactgga aggcgcaggg aatgtgttcc tgggaagcaa ccgtctgact
     5581 gtggggagca acaacctgag cacaacattt tctggcgtga ttgcggatta ccctggtgat
     5641 cctatcggct attctggctc gttgagcaaa actgggaaag gcaagctgac actgagcaac
     5701 acaaatctct acgacggcgg gacgacaatc gacaacggca cgcttgtggt caaaaatggg
     5761 tctggctcag ccacaggcag cggacctgtg caggtgaact ccggcacgct gagcggtaaa
     5821 ggtagcatca gcggcgcagt cacggttggt aatggcacga ccgcaggcgc catccttcac
     5881 cctggcatcg gggccaagcc gagcagatta ctcattaaca ataccgtcag ctttaatgcc
     5941 acctcggctt tcaagtgtta cgtcaagcgt tcaaccacac cagtcgccgg caagttgagt
     6001 gcgctaggag tgactatcgg cacgaacgtg agcttcacgt tgctagagac cggcacgggg
     6061 acattaacgg ccgggaccgt cttcaccgtc atcaagaaca cctcagctaa cccaattttc
     6121 ggcaggttca gcaacctgcc tgatggcgga acaatcacga ccgttaacgg cactgtctac
     6181 aaagcgaact acagcggcgg caccgg
//
LOCUS       DASTDG010000023         7910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_249393, whole genome shotgun sequence.
ACCESSION   DASTDG010000023 DASTDG010000000
VERSION     DASTDG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249393"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFO30_00840"
     CDS             <1..546
                     /locus_tag="VFO30_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9417861.1"
                     /translation="PHVGFFSGYEVYNERDPEVGQEFNYGYVFGINGTWHIFDGFATK
                     GRLQATRARRQAAVEALAAARRSVASEVRSAFFDLEQARRVLESETKNVQTADESLEI
                     AKSNFAAGLATQLDVLQSAADVTRTRTTRLTAIHLHNAALARLARACACTPDALDFAA
                     KADEDAKVSQLAEPPSNLTQP"
     gene            669..1967
                     /locus_tag="VFO30_00845"
     CDS             669..1967
                     /locus_tag="VFO30_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9417862.1"
                     /translation="MLSVIALAQSANAITLDAVLDRTLEKNPAIRQAKANVEQAAGQR
                     LVLRSIMWPDVKLLVPAGVQGGHRAGEGGVKAFGFVRVFFTQQLLNAAIAPSRRLGDV
                     DLLIAQQQLNVAVVEQLHAARLAFYTALYTRELQSIQEDQRHRFEQNVASQKSRYEAG
                     LAQRSDFVAATVEASELTSAIETAKRARETARLQLAEAMGENPTAESALPEPEGELQF
                     APVNIDVKSATARALEERADLKLARLIAGAANEQQRIIEAGYYPAVAAHITGDYIPVS
                     GIHREGSTRRTDDFVGSEVREGAAYTWRLIDNGKVGGAVMKQRAAREMNELVCRQLEA
                     NVGRELSRIRDQLSAIEGRQKALAGASTTAEESAKLVAQNLAGGLASQLEYRVTQNGS
                     LQTKSGLLTAIYEHNVARAEWDRATGRYFQFSDQSVPADH"
     gene            2095..3246
                     /locus_tag="VFO30_00850"
     CDS             2095..3246
                     /locus_tag="VFO30_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9417863.1"
                     /translation="MNLPERRVLWVGGTALVLLFGLTYYWFMPVAEVAAVRRGTAISA
                     VYGTVRIEPTFVIAIRAQNAGYIKLADTLAAGRGAIGKKVENGELLATIADETTARQL
                     RQARADLEAAKQRAALPLPSEEPLKAAEDNLRRLEQVVASGNVPTVEYERAKNEANRL
                     RSVLETERIERNRNLDQLEDTVKKLEAQMKNSEVRSRMDGLLTNIQVIDGQLVSEGNE
                     LFTVASRKNYVRGEVNEEDVGEVKSGMQATLQLYAYRTKTFTAHVTAIQPAADPTTQR
                     YTIALDLENPPDNLMAGMTGEMNIITGTHQNALLVPTRALIVDQALAVKRGIVEKRTV
                     KVGYRTLDYAEALSGLSQGDYVVVTNQDRLRPGQPVRKRLIKSPPPPKL"
     gene            3243..4478
                     /locus_tag="VFO30_00855"
     CDS             3243..4478
                     /locus_tag="VFO30_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417864.1"
                     /translation="MTPPLYIALRFLMHRKRALLLSLSGVVFGVGIFICTQAQTQGFA
                     QHFINSTIGSNGALVVRSRFRPRYPSLAVPSRSSKPPALRLQFEGITNANEIMRVSRQ
                     FSNVVSCSPVLRGTLSARAAFQNATVDLFGIDPASHAHTTDILQQLIAGKYDDFRNNT
                     TSLIVGSRLAEFLGVQTGDTMQLLSPSGEYWRFTVAGIARAGVGAIDATRVYAHARVA
                     QTLLQRPFGASIIIYKLRNPDRAPALAKHFENLFQHSAESWQDREESTLQLFLTLRAS
                     AAITVSLIILLAGFGIFNVLTMSVLSKVKEIAILRSMGYRRIDISAIFLWQGALIAAA
                     GSLLGCALGAVLTWMIGRIPIRIRGLIYADHFLVSWDWRHYLSATLLAIVAVFIASYV
                     PARRAALLAPVATLRGSSA"
     gene            4535..5587
                     /locus_tag="VFO30_00860"
     CDS             4535..5587
                     /locus_tag="VFO30_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9417865.1"
                     /translation="MKLLDKIGREFSGYNALESAKRLFDRRPLQCSLYLTDRCNLDCS
                     YCTEYDNSRPHPKLEDLKTWIAKIRELGTTRIALVGGEPLLHPDVVEIVRYCRELGFA
                     TSLTTNGFLLTRELLRELENAGLQVMQISVDRMTPSPVTKKSFKTILPKLDYFKDSNI
                     SLHITGVICQDTLPESRAVLETGLARGIPTEVRLVHADPTNRFRVERGSRDELEAFID
                     SLIERKRRGERIHTTETMLNYQRSLLRNEPVKWTCMAGYKLFFVSAQGKFWICSMVHT
                     DKHIMDVTLDDLYDNNRKKSCQAGCGVYCAVGASLLVQNPTKVITNEIVARARRLPTL
                     LKQAVAPARRREAAPI"
     gene            5655..6371
                     /locus_tag="VFO30_00865"
     CDS             5655..6371
                     /locus_tag="VFO30_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9417866.1"
                     /translation="MNGQIALRCRDLDRYLGEEEERVHALRGVSLDLGLGSVHAIVGP
                     SGCGKSTLLYVMGLLDPPDAGSVTIDDQTISHLSDDELARKRNEFIGFIFQFHFLMED
                     FTAQENVMIPMRKLARYSDQEMRERAAALLEAVGLGDKLRRPSRHLSGGEQQRVAIAR
                     ALANDPRVILADEPTGNLDTANSERAFELLQRLVRAGGKALLLATHNRAVAEACDWIH
                     EMQDGQIIASHARETRKSRF"
     gene            complement(6368..7678)
                     /locus_tag="VFO30_00870"
     CDS             complement(6368..7678)
                     /locus_tag="VFO30_00870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9417867.1"
                     /translation="MSESVLPPLIIVAHVPDALTDLCGVSLLERLRRIVRQIGFGEAT
                     ILSNSSESVAAHVAMQTWRRADVSLSYRQQQSRSAVVGDILGCLAAMNVRRRIGAFVA
                     FAHFYCDPRLLRALAHRPNDTAIVDSDPPPMIAPLWKNAGGSPSCAAVLSNEWLSGKE
                     PAAELSAAIGFALSNDGIALLDAAQQDGYVKSMRRIVRPIFFPAPNADLRPLAERVLM
                     EATQNGVLDLPALAHAPIEKWIVAHLCRTAITPNQITLLTGLVGVAVTFLYAAGYLWT
                     GALLALAIGVLDGVDGKLARVKVQTTKLGKAEHTLDLFIEMSWWAALAYHFVSTGQLH
                     YAYSILIGFYASHFLARLARDRIQRRIGRSLDDFAPFDRLVRSVAGRRNIYTWLFTLS
                     LLLGTPATGFVLLCSWGILSATVHICRSIQIGLTLRHPVAATPS"
BASE COUNT         1704 a         2312 c         2438 g         1456 t
ORIGIN      
        1 ccgcacgtcg ggtttttctc gggctacgaa gtttacaacg agcgcgaccc ggaggtcgga
       61 caagagttca actacgggta cgtgttcggc atcaacggca cgtggcatat ttttgatggc
      121 ttcgcgacca aaggccggct ccaggcgacg cgagctcggc gccaagctgc cgtcgaagcg
      181 ctggcggcgg ctcggcggag tgtcgcgtca gaagtgagaa gcgcgttttt cgatctcgag
      241 caggcgcgac gcgttctcga atcggagacc aaaaacgtgc agacggcgga tgaatcgctg
      301 gagatcgcga aaagcaactt cgcggcggga ctcgctacac agctcgacgt tctgcaatcg
      361 gcagccgacg tgacacgaac gcgcacgacg cgcttgactg cgatccatct gcacaacgct
      421 gcgctggcgc ggctcgcgcg cgcctgcgcc tgcacgccgg acgcgctcga tttcgcagcg
      481 aaagcagacg aagacgcgaa agtttcgcag ctggccgagc cgccgtcgaa cttaactcag
      541 ccatgaaacg cgcgcgaaag gtggatcgag cagtccgttg ctcgatgcta attcgatgtg
      601 gccgtaggcc accactttct gccatcgccc gcgcagcggt cgatccacct cgcgctggac
      661 tctgtgttat gctctcggtg attgcactgg cgcaaagcgc gaacgcaatc acgctggatg
      721 ccgtcctcga ccggacgctg gagaaaaatc cagcaatccg gcaggcaaaa gcgaacgtgg
      781 agcaagcggc agggcagcga ctcgtgctgc gctcgatcat gtggccggac gtgaaactcc
      841 tggtgccagc cggcgtccaa ggcggacacc gcgcgggtga agggggagtt aaagctttcg
      901 gattcgtgcg cgtgtttttc acccaacagt tgctgaacgc ggcgatcgcg ccgtcacggc
      961 gactgggaga tgtcgatctt ttgattgcac agcagcaact caatgtcgcg gttgtggaac
     1021 aactgcacgc ggcgcggctg gcgttttaca ccgcgctgta cactcgcgag ctgcaatcga
     1081 ttcaggaaga tcagcggcac cggtttgagc aaaacgtcgc cagccagaaa agtcgctacg
     1141 aagcgggatt ggcgcagcgc agtgatttcg tcgctgcaac tgtggaagcg agtgagctta
     1201 cgtcagcgat cgagacggca aagcgagccc gggaaacggc gcgactgcaa ctggccgaag
     1261 caatgggcga aaatccaacg gctgagtcag cattgcccga acccgaaggc gaattgcagt
     1321 tcgcgccggt gaatatcgat gttaaatcgg cgacggcccg cgcgctggaa gaacgcgccg
     1381 atctcaagct ggctcgattg atcgcaggcg cagcgaatga gcaacagcgg atcatcgaag
     1441 cgggctatta cccggccgtc gcggcgcaca tcaccgggga ttacatcccg gtgtccggaa
     1501 tccatcgcga gggttccacg cgccggactg acgatttcgt cggctcggag gtgcgcgaag
     1561 gcgccgccta cacctggcgg ctgatcgata acggcaaagt gggcggcgcg gtcatgaagc
     1621 agcgcgccgc gcgggagatg aacgagctag tttgtcggca attggaagcg aacgtcggcc
     1681 gcgaactgtc gcggattcgc gaccagctca gcgcaatcga gggacggcaa aaagcgctcg
     1741 ccggcgcgag cacgacggca gaagagagcg cgaagcttgt cgcccaaaat ttggccggcg
     1801 gacttgcttc gcagctcgag tatcgcgtga cgcagaacgg ttctctgcaa accaagagcg
     1861 ggttgcttac ggcaatctac gagcacaatg tcgcccgggc cgagtgggac cgcgcgaccg
     1921 gccggtactt tcaattttca gatcagagcg ttccggcgga tcattgaaac gctctgtagc
     1981 cacggcgctg tgcgccgttg agtgaaattg caatgaaaga cggccgcagg cccgtggcta
     2041 cggaccggct acagcggtag caatggctta gaggatgtgt tctagttcgg gcgcttgaac
     2101 ttgccggaaa gacgcgtgtt gtgggtcggc ggcaccgcgc tcgtgctgct gtttgggctc
     2161 acgtattact ggttcatgcc ggtcgccgaa gtggcggcgg tgcgacgcgg cacggcgatc
     2221 tcggcagtct acggcacggt gcgaatcgag ccgacgttcg tcatcgcgat tcgggcacaa
     2281 aacgccggat acataaaact cgcggacacg ctcgcggccg ggcgcggcgc tatcggtaag
     2341 aaggtggaaa atggtgaatt gctggcgacg attgccgacg agacgaccgc gcgacaattg
     2401 agacaagccc gtgccgatct cgaagcggcg aaacaacgcg cggccttgcc gctgccgtcg
     2461 gaggagccgt tgaaagccgc ggaggacaat ctgcggcggc tcgagcaagt ggtcgcctca
     2521 ggtaacgtac cgacagtcga atacgaaaga gcaaagaatg aggcaaaccg gttgcgcagt
     2581 gtgctcgaga cggaacggat cgagcgtaat cgaaatctgg atcagctcga ggatacggtg
     2641 aaaaagcttg aagcgcagat gaagaactcg gaggttcgct cacgcatgga tgggctgctg
     2701 accaacattc aggtgatcga tggacagctc gtctcggagg gcaacgagct ttttaccgtg
     2761 gcctcccgca agaattacgt acgcggtgaa gtcaacgagg aagatgtcgg cgaagtgaaa
     2821 tcagggatgc aagcgacgct gcagttgtat gcctaccgca cgaaaacgtt caccgcccac
     2881 gtgaccgcga tccaaccggc ggcggacccg acgacgcagc gttacaccat cgcactcgac
     2941 ctggaaaatc ctccggacaa tctaatggcg ggcatgacgg gcgagatgaa catcatcacc
     3001 ggcacgcacc agaacgcctt gctcgtgccg acgcgcgcgt tgattgtcga tcaagcgctg
     3061 gcggtcaagc gcgggatcgt ggaaaagcgc acggtcaaag tcggctatcg cacactcgat
     3121 tacgcggagg cactgagtgg cctgtcgcaa ggcgactacg tcgttgtcac aaatcaggac
     3181 agactgcgcc cgggccagcc ggtgcggaaa cgcctcatca agtcgccgcc gccgccgaag
     3241 ttgtgacccc gccgctgtat atcgcgctgc gctttttgat gcatcgcaag cgcgcgctgc
     3301 tgctcagcct gagcggcgtc gtgttcgggg ttggcatctt catttgcaca caagcgcaaa
     3361 cgcaaggttt tgcgcagcat ttcatcaact cgaccatcgg gagcaacggc gcactcgtgg
     3421 tgcggtcgcg cttccggccc cgctacccaa gtctggcggt gccatcgcgg agctcgaagc
     3481 cgccggcact tcgtctgcag ttcgaaggga taacgaacgc gaacgagatc atgcgtgtga
     3541 gccggcagtt ctcgaacgtc gtatcgtgct cgccggtgct gcgcggcacc ctcagcgcgc
     3601 gcgccgcatt ccaaaatgca actgtcgatc ttttcggcat cgacccggca tcacatgcgc
     3661 acacgacgga cattttgcag cagctcatcg ccggcaagta cgacgatttt cgcaacaaca
     3721 cgacttcact tatcgtcggt tcgcgactcg cagagttcct cggcgtgcag actggcgaca
     3781 cgatgcagct gctctcgccg agtggcgagt actggcgctt taccgtggcg ggaattgcgc
     3841 gcgccggggt gggggcgatc gacgcgacgc gcgtctatgc gcacgcgcgc gttgcgcaaa
     3901 cgctgctgca gcggccgttc ggcgcgtcga tcatcattta caaactgcgc aaccccgatc
     3961 gggcaccggc actggcaaaa catttcgaaa acttatttca gcacagcgcc gaaagctggc
     4021 aggaccgcga agaatcgacg ctgcagttgt tcctcacctt gcgcgcctcg gccgcgatca
     4081 ccgtttcgct catcatcctg ctggccgggt tcggcatctt caacgtgctg accatgtcag
     4141 tcctgtcgaa agtgaaggag atcgcgattt tgcgctcgat gggttatcga aggatcgaca
     4201 tctccgcaat ttttctctgg caaggcgcgc tcatcgccgc ggccggctca ttgctcgggt
     4261 gcgcgctcgg cgcggtgttg acctggatga tcggtcggat tccaattcgc atccgcggct
     4321 tgatctacgc tgaccatttt ctggtgagct gggactggcg gcactacctt tcggcgacgc
     4381 tgctcgctat cgtcgcggtc ttcatcgcca gctatgttcc cgcccgtcgc gctgcgttgc
     4441 tcgcgccagt ggccacattg cgcggctcaa gcgcataaac gcgcacgtga cacgagccga
     4501 tagcccggta aactcttcgt ccccacgtag tacgatgaag ttgctcgata aaatcggccg
     4561 cgaattttct ggctataacg cgcttgaatc cgccaagcga ctattcgatc gccggccgct
     4621 ccagtgcagt ctttacttga ccgaccgctg caatctcgac tgctcctact gcacggagta
     4681 cgacaactcg cggccgcatc cgaagctcga agacctgaag acctggatcg ccaaaattcg
     4741 cgagctcggc acgacgcgga tcgcactggt gggcggcgaa ccgctgctgc accctgacgt
     4801 ggtcgagatc gtgcgctact gccgcgagct cgggttcgcc acgagtctga cgacaaatgg
     4861 ttttctgctc acacgagaac tcttgcgcga actggaaaat gcaggcctgc aggtgatgca
     4921 gatcagtgtc gaccgcatga cgccgagtcc ggtgacgaaa aaatcgttca agacaatttt
     4981 gccgaagttg gattacttca aggactcgaa cattagcctg cacatcaccg gcgtgatctg
     5041 tcaggacacg ttgccggagt cgcgagcggt tctagagacg ggcctcgcgc gcggcattcc
     5101 gaccgaagtg cgcctcgtgc acgcggaccc gacaaaccgg ttccgcgttg agcgtggctc
     5161 gcgcgacgag ctggaagcat ttatcgactc gttaatcgag cggaaacggc gcggtgagag
     5221 gatccacacg accgaaacga tgctgaacta tcaacgtagt ctgttgcgga atgagccggt
     5281 gaaatggacc tgcatggccg gctacaagct tttcttcgta tcggcgcagg ggaagttctg
     5341 gatatgcagc atggtgcaca cggacaaaca catcatggat gtgacgctgg acgatcttta
     5401 tgacaacaat cgcaagaaat cgtgccaggc cgggtgtggc gtgtattgcg ccgtcggcgc
     5461 gtccttgctc gtgcaaaatc ccacaaaggt aatcacgaac gaaattgtcg cgcgcgcccg
     5521 ccgtcttccg actctcctca aacaggcagt ggccccagcg cgtcggcgcg aagccgctcc
     5581 gatctgatca atgtgtgaag cgcatcggat ggcgtgcgcg tcagcaaatc attaggctac
     5641 gcgcagagta agagatgaat ggacaaatcg ctcttcgctg ccgcgatctg gaccgttatc
     5701 tcggggaaga ggaagagcgc gtccacgcgt tgcgcggcgt ctcactcgac ctcgggctgg
     5761 gcagtgtgca cgccatcgtc ggtccgtccg gctgcggcaa aagcaccttg ctttacgtga
     5821 tgggtctgct cgatccaccg gatgctgggt cggtcaccat cgacgaccaa accatctcgc
     5881 acctgagcga tgacgagctc gcacgaaaac gaaacgaatt catcggcttc attttccagt
     5941 tccatttttt gatggaggac ttcaccgcgc aggaaaatgt catgatcccg atgcgcaaat
     6001 tagcgcggta ttcagaccag gaaatgcgcg aacgcgcggc cgcattgctc gaagcggtgg
     6061 gcttaggcga caaactgcgg cgcccgagcc ggcatctttc gggcggcgaa caacagcgcg
     6121 tggccatcgc gcgcgctctg gcaaacgatc cgcgcgtgat cctggccgac gagccgaccg
     6181 gcaatctcga cacggcaaat tcggagcgcg cctttgagct tttgcaacgg ctcgtgcgcg
     6241 caggcgggaa agcattgctg cttgcgacgc acaaccgggc ggtcgcggag gcgtgtgatt
     6301 ggattcacga aatgcaggat ggtcagatca tcgccagcca tgcgcgtgaa acacgcaagt
     6361 cgaggttcta gctcggcgtc gccgcgactg ggtggcgtag agtgaggccg atttgaatcg
     6421 agcggcaaat gtgcaccgtc gcactgagga ttccccagct gcaaaggagg acaaatcccg
     6481 tcgctggggt gccgagcaaa agactgagcg tgaacagcca ggtgtaaatg tttcgccgcc
     6541 cagcgacgga tcgcacgagc cggtcgaacg gcgcgaagtc atccaggctg cggccgatgc
     6601 ggcgttggat gcggtcgcgg gcgagacgcg cgaggaaatg cgatgcgtag aagccaatca
     6661 aaattgagta ggcataatga agctggcccg tgctgacgaa atggtaagcc agcgccgccc
     6721 accacgacat ttcgatgaac aagtcgagcg tgtgctcggc tttgccgagc ttcgtcgtct
     6781 gcactttcac ccgcgccagt ttcccatcca ccccgtcgag aactccgatc gccagtgcga
     6841 gcaacgctcc cgtccagaga tatccagccg catagagaaa cgtcacggcc acgccgacca
     6901 agcctgtgag gagtgtaatt tggttgggcg tgattgctgt ccggcacaaa tgcgctacga
     6961 tccatttttc aatcggcgcg tgcgctagcg cgggcaaatc gagcacgccg ttctgtgttg
     7021 cctccatcaa cacacgctct gcgagtgggc gcaaatccgc gttaggagcg gggaaaaaga
     7081 tcgggcgcac aattcggcgc atgcttttga catagccgtc ctgttgcgca gcatcgagga
     7141 gagcgatgcc gtcgttcgat agagcgaatc caatcgctgc gcttagctca gcggcaggct
     7201 cttttccgga caaccattcg tttgacagca cagcggcaca ggaaggcgag ccgccggcat
     7261 ttttccaaag cggcgcaatc atcggaggcg gatctgaatc aacaatcgcg gtgtcgttag
     7321 gccgatgtgc gagcgctcgt aagagccgcg ggtcgcaata gaaatgtgcg aatgcgacga
     7381 aagcaccgat tcttcgccga acgttcattg cggcgaggca acccaaaatg tccccgacca
     7441 ccgccgatct gctttgctgc tgccgataac ttagcgatac gtctgcgcgc cgccaggtct
     7501 gcatggcgac atgcgcagca acactttcag aggagttcga cagaatcgtc gcctcgccaa
     7561 atccgatctg ccgcacaatg cgccgcagcc gctccagcaa cgatactccg cagagatcgg
     7621 tcagcgcgtc gggaacgtga gccacaataa tcaacggcgg caaaacggat tcgctcatcg
     7681 caattgtcga tctaaagtga gcgcgaagtt aatcgaacgc gccgtcccag caataagcac
     7741 gggcgaaatg tttgctgccg ccatcacgcc gaccgacgtg cggtcaatgt cgatcaaacg
     7801 agcgccgacc tgacgcgcaa tagtgttgcg agaattgcgc tcaatcgatg acggacttca
     7861 ccttgtgcat gatcaaccgg ctggtcgaac ctggcgcatt cgataaataa
//
LOCUS       DASTDG010000024         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_986203, whole genome shotgun sequence.
ACCESSION   DASTDG010000024 DASTDG010000000
VERSION     DASTDG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986203"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFO30_00875"
     CDS             complement(<1..446)
                     /locus_tag="VFO30_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417868.1"
                     /translation="MAPPFGFSPAELRKLRALKTPAGVQHFLDELPYNLEYTARSPRR
                     VLEDRTGSCLEGAIFAAAALRVLGFPPLIFDLEAEQDTDHVVALFKMRGRWGAVAKSN
                     FTGCRYREPVHRALRELAMTYFNVYFNLRGERTLRRYSRPVNLARF"
     gene            531..806
                     /locus_tag="VFO30_00880"
     CDS             531..806
                     /locus_tag="VFO30_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET9417869.1"
                     /translation="MPKYVIEREIPNAGKLSESELQGISQKSCGVLRNLGPQIQWVQS
                     YVTDDKVYCVYIAPNEEMVREHAKQGGFPANRVSEVKTVIDPTTAET"
     gene            831..1343
                     /locus_tag="VFO30_00885"
     CDS             831..1343
                     /locus_tag="VFO30_00885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9417870.1"
                     /translation="MDAAELKSLQAPLKERYRDHPETALITLRARGRTGDNVTCKIET
                     GKAHVEAGLHPATGGDGTTACSADMLLESLVACAGVTLSAVATALSIGLRGAIVEAEG
                     DLDFRGTLGIAKDVPVGFKQIRLHFDLDTDASSEQISTLLRLTERYCVIYQTLTHPPK
                     IDISHAVTSV"
     gene            1354..2208
                     /locus_tag="VFO30_00890"
     CDS             1354..2208
                     /locus_tag="VFO30_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET9417871.1"
                     /translation="MPLYLDIHDLHGPINPDDLAKAHEKDVEVEQKYGVNYLKYWVNE
                     SRGKVFCLVDAPNPEAADRVHKEAHGLVAEKMIEVDPELADGFLGGGEVNAAGAVLLP
                     GGAAAGRDPAMRTIVFTDIVESTAMTQSMGDDAAMEVLNLHNSVVRNALADAGGREVK
                     HTGDGIMASFISAAAAVKCASQVQRDLARHDKNRDRSVKVRIGAAAGEPVENDNDLFG
                     CTVQLAARLCAQAEPEQILVSNAVVELCLGKKFLFRDAGKFALRGFDQPIRGHAVVWA
                     DESVVSAS"
     gene            complement(2302..2826)
                     /locus_tag="VFO30_00895"
     CDS             complement(2302..2826)
                     /locus_tag="VFO30_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417872.1"
                     /translation="MDSALLKSWFGALISAVVISTACADTFPASGPIVPGSVARLRFS
                     KAAAPARAPVAVKRAIWAANQLATKPYRFGGGHRSFHDRGYDCSGTVSYALGGAGLIK
                     SPMNSSDFRRYGARGRGRWITIYTRPGHTYAVIAGLRLDTTPWDNYTGRWAPRWQTNE
                     RVPLRFEARHPIGL"
     gene            3027..3941
                     /locus_tag="VFO30_00900"
     CDS             3027..3941
                     /locus_tag="VFO30_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9417873.1"
                     /translation="MVRKVNSPVVKALRVVWRVTQRLVWETATDNTTGLAAQMAYLLL
                     IALAPGLLFLWHLLGLFGTDPAKLHGMFSILKSFMPPDPKVQDILDSAAASVVVTGSS
                     GLLANAGIILGIYFGTVFIATISRALSETYGVHENRHWWSKYIISFLLLFWFGVAIFL
                     CFSAVVFGETLAGVAEVNFQLRFPLQEWVAALNLPFTAIALIGLALVLYLLTPENYLT
                     IRQALPGAIFFSLGWILVTKLFQFYVAKYDRYNPTYLALASIIMLLTWMYLTCLFLLL
                     GGKLNAILRRERERGRVELGPTPAAQPA"
     gene            4017..5090
                     /locus_tag="VFO30_00905"
     CDS             4017..5090
                     /locus_tag="VFO30_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HET9417874.1"
                     /translation="MAALLVFAAFTGSVCLRAQLTEEETPAHSFKSRPTPTPTPKPKT
                     KKKKKVSPTPTPTPKPSKTPEASVTPTATPTKTPKPKAKSTAKKSPAKVGGAAKKKKK
                     TTSPLPGGSLTEKARSPRPSETPEIRSALPVVSPPPSSLPPVAPPVARSTTPFAAPAE
                     EEPELTIKKSDYEQRSFWARLFGFGRRRSGYRYLTRSVRAAIDRAPVMRGRWQFIAVH
                     NSGTRQGNAAVFDYYHRRVRRMPNGLAYHFVIGNGTSTGNGEIEVGDRWRRQINGGHV
                     HSDYLNSIALGICLVGDFNRDQPTRAQLDSCEELIRYLRQRCGKIDNHYPIVKPHREL
                     NPPRWPTDCPGDAFPYTWFRRFQ"
     gene            complement(5091..6146)
                     /locus_tag="VFO30_00910"
     CDS             complement(5091..6146)
                     /locus_tag="VFO30_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417875.1"
                     /translation="MIGTLALRAQQIPVPDPNDTARFLAGMPLPKDSPLYPLTNQPAW
                     QAHASYFEHAFSKLSRGKLAKLHVWQRQYLPESLEPLPVAYYMFSGPDFLYVDQFFPN
                     ASVYIMCGKEALGPAPDPLRVADLGWALQNLENAMKASLTTTYFITQEMKVDLQQQQL
                     NGTLPILYVFLARGDKTIRAVTFVRLDSAGNEHESAPGAGGTPGVRIDYTDNASGREQ
                     TVYYFTTDISDGGIGANPAFMKFCGRHGVGASFLKSSSYLMFEEGFARVRNFILDHSN
                     IVVQDDSGIPISYFDRNKWNLRFFGTYIGPIELFKKYSQPKLAELYQVSNPAPFDIGF
                     GYQWDFHKSNLTVAERQ"
     gene            complement(6298..7635)
                     /locus_tag="VFO30_00915"
     CDS             complement(6298..7635)
                     /locus_tag="VFO30_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9417876.1"
                     /translation="MSRVTDLATAQKREPRRTIGFLTACGIVIANIIGTGVFTSLGFQ
                     LADIRSGFALLMLWIIGGIAALCGALCYGELSAALPRSGGEYHFLSRIYHPALGFMAG
                     IVSATVGFAAPIALTAIAFGKYFHGVFGVGAPLVFSFAVVWIVALFHLGNLRVGSVFQ
                     NLWTLVKVLLIGALIGAGLLVEPKQPIDFLPQTGDTMSILSGAFAVALVYVMYSYSGW
                     NAATYITGEITRPEKNVPRALLIGTSVVMVLYVLLNGVFLLCSPREELSGQLEVALIA
                     GKHIFGLNGGRIVGGVICLGLVSAISSMTWLGPRVTMAMGEDHWLLRLLGRKNRHGVP
                     TNAALVQLLIVNLFFAKNFQQIVIYIQFSLLLCSLLTVVGVIVLRVTSPDLPRPYRVR
                     LYPLPPVVFAAVTIWMMVYLLRSHPGESAAGLATILLGTLLYFAAGKKTRPTA"
     gene            7756..8175
                     /locus_tag="VFO30_00920"
     CDS             7756..8175
                     /locus_tag="VFO30_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9417877.1"
                     /translation="MNSQLNLSVEPATQLAVFLANRPGALTRVCAALAQAGINVHALT
                     TSDTVDHSVVRMVVSDPTKALMLLGEAGALALEADVLMIEGENQPGVLERIAARLAEA
                     RVNIEYLYLASSPKAERGLLVLRPSDVEKAQRVLKDL"
     gene            8234..>8631
                     /locus_tag="VFO30_00925"
     CDS             8234..>8631
                     /locus_tag="VFO30_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HET9417878.1"
                     /translation="MTPIKAWPGRPYPLGATWLGNGVNFALFSQRATAVELCLFDSLD
                     ATEENIRVPVIERTNYIWHVFLPDARPGQLYGFRVAGSYEPERGLRFNSSKLLLDPYA
                     KAIAGEINWADEMFGYVVGDELEDLSCDFR"
BASE COUNT         1850 a         2522 c         2562 g         1697 t
ORIGIN      
        1 tcgaatcggg ccaaattcac cgggcgcgaa tagcggcgca gggtgcgttc gccgcgcaga
       61 ttgaagtaga cgttgaagta ggtcatggcc agctcgcgca aggcgcgatg cacaggctca
      121 cgatagcggc agccggtgaa atttgatttt gctactgctc cccaacggcc gcgcattttg
      181 aaaagcgcca cgacgtgatc ggtgtcctgt tccgcttcca agtcaaagat cagcggcgga
      241 aacccaagca cgcgcagcgc ggcggcggca aaaatcgcgc cttcgaggca ggacccggtg
      301 cgatcttcca ggacgcgccg cggcgaacgt gcggtgtatt caaggttgta gggcaactcg
      361 tcgagaaaat gttgcacgcc cgcaggcgtt ttcagagcgc gcagtttgcg caactctgcc
      421 ggtgagaacc cgaagggtgg cgccatgatt gtcgagcttc gacggcttga tctttgattg
      481 cggtgtcgcg gtttgacttt ggcgactgtt ttcactacac gaataaacct atgccgaaat
      541 atgtcatcga acgcgaaatt ccgaatgccg gaaagttatc ggagtccgaa ttgcagggta
      601 tatcgcagaa atcatgcgga gtgctgcgca atctcggtcc gcagattcag tgggtgcaga
      661 gttatgtgac cgacgacaaa gtttactgcg tttacatcgc gccgaatgaa gagatggtgc
      721 gcgaacatgc caagcagggc ggttttccag cgaaccgcgt ttcggaagtt aaaacggtga
      781 tcgatccgac aacggccgag acctgacctg cagatatcgg tcgcagaacg gtggacgctg
      841 ctgagctcaa atctttgcag gcgccgctca aagagcggta tcgcgaccat cctgaaacgg
      901 cgttaatcac gctgcgcgca cggggccgca ctggcgacaa cgtcacctgc aaaattgaaa
      961 ccggaaaagc gcacgtggag gccggactgc accccgcgac cggcggtgat ggcacaaccg
     1021 catgctcggc cgacatgctg ctcgaatcgc tcgtcgcttg cgcgggtgtg acgctgagcg
     1081 cggtcgcgac tgctctgagt atcggattgc gcggtgcaat cgttgaagcc gaaggcgatt
     1141 tggattttcg cggaacgtta ggcatcgcaa aagatgtgcc ggttgggttt aagcagattc
     1201 ggttgcactt cgatctggac acggacgcgt ccagcgaaca gatcagcaca ttgcttcggc
     1261 tcaccgaacg ttactgcgtc atttaccaaa cgttgactca tcctccgaag atcgacattt
     1321 cgcatgccgt aacatcagtc taacctccac gatatgccgt tatacttaga cattcacgac
     1381 ctccatggcc cgattaaccc tgatgattta gccaaggcgc acgagaagga tgtggaggtc
     1441 gaacagaagt acggtgtcaa ttacctgaag tattgggtca acgagagtcg cgggaaagtt
     1501 ttctgtctgg ttgacgcgcc gaaccccgaa gcggcggacc gcgttcacaa ggaggcgcac
     1561 ggtctggtgg cggagaaaat gatcgaagtt gaccctgagc tcgccgatgg attcctcggt
     1621 ggtggtgaag tgaacgccgc gggagcggtg ttgctgcccg gtggagcggc ggctggccgc
     1681 gacccagcga tgcggacgat tgtgttcacc gacatcgttg aatcgaccgc catgactcaa
     1741 tcaatgggcg atgacgcagc gatggaggtg ctcaatttgc acaacagcgt tgtgcgaaat
     1801 gccttggcgg atgccggtgg ccgcgaagtc aaacacacgg gggatggcat tatggcgtcg
     1861 ttcatttcgg ccgcagccgc ggtcaaatgc gccagccagg tgcaacgcga tctcgcccgc
     1921 cacgacaaga accgtgatcg cagtgtcaaa gtgcgaatcg gcgcggcggc gggcgaaccg
     1981 gtcgagaacg acaacgatct tttcgggtgc actgtccagc tggcagcgag actttgcgcc
     2041 caggcagaac cggaacagat ccttgtttca aacgcggtag tcgagctctg cctcggaaag
     2101 aagttcctat ttcgagacgc cggcaaattt gctttgaggg gctttgatca accgatccgc
     2161 ggtcatgccg tcgtatgggc ggatgagtca gtagtttccg caagctgatt gtagccacgg
     2221 ccctgcgggc cgtctctctc ctctgacgcg ccacccgcct tcgcaaagct atggcgtggc
     2281 aagcaggcgc gtggctacag cctaaaggcc gatcgggtgt cgcgcttcga atcgcaacgg
     2341 cacgcgctcg ttcgtttgcc agcgtggtgc ccagcggccc gtgtaattgt cccatggcgt
     2401 cgtgtcgagc cgcaggccgg cgatgacggc gtatgtgtga ccgggccgcg tataaatcgt
     2461 gatccacctc ccgcgaccgc gcgcgccgta tctgcggaaa tcggatgaat tcatcggcga
     2521 tttgattaag ccggcgccac cgagtgcgta cgacaccgtg ccggaacaat cgtagccgcg
     2581 atcgtgaaag gagcgatgtc cgccaccgaa ccggtacggt ttggtcgcaa gttggttcgc
     2641 agcccagatg gcgcgtttca ccgcgaccgg cgcgcgcgcc ggtgcggctg ctttactgaa
     2701 acgcaaccgg gcaacgctac caggaacaat cggacccgat gcgggaaacg tgtctgcgca
     2761 tgccgtcgag attaccacgg cagaaatcag cgccccgaac cagcttttga gaagagcgga
     2821 atccatgcaa ctctctgagc gcgcgtgata tgtcacattt gccgctactg aaaaagaaat
     2881 tttttgttct acttgccctg attgccagcc ctgtgcagcc cccgcgccgc cgacgagaaa
     2941 gttggtctat cccaatgcac cgacggaaat actttgccag aacacagcat cgctcccgtg
     3001 cgagcgcctc aagttgatgg atatttgtgg tgcgcaaagt aaactctccg gtggtgaaag
     3061 cgctgcgtgt tgtctggcgt gtgacccagc gcctggtctg ggaaaccgcg accgataaca
     3121 cgaccggtct ggccgcgcag atggcttatc tgttgctcat cgcgcttgcg cccgggctgt
     3181 tgtttctctg gcatttgtta ggcctgttcg gcaccgaccc agcgaagctg catggcatgt
     3241 tctccattct gaagagcttc atgccgccgg atccgaaggt gcaggacatt ctcgacagcg
     3301 ccgcggcgag cgtggtcgtg accggctcca gcgggttgct cgcgaacgcc ggaatcattc
     3361 ttggcattta tttcggcacg gtttttatcg cgacgatctc gcgcgcgttg agcgagacct
     3421 acggcgtgca cgagaaccgt cactggtggt cgaaatacat catctcgttc ctgctgctct
     3481 tctggttcgg cgtcgccatt tttctctgct ttagcgcagt cgtttttggc gaaaccctgg
     3541 cgggcgtggc cgaggtaaat tttcagcttc gttttccgtt acaggagtgg gtcgcggcgt
     3601 tgaacctgcc cttcacggcg atcgcgctca tcggtctcgc cctcgtcttg tatctactga
     3661 ccccggagaa ttacctgacc atccggcaag ccttacccgg ggccatcttt ttttcgttag
     3721 gctggatcct ggtgacgaag ctgttccagt tctacgtcgc gaagtacgac cgctataacc
     3781 cgacgtatct ggcgctcgcg tccatcatca tgctgctcac ctggatgtat cttacctgcc
     3841 tgtttctgtt gctcggcggc aaattgaacg ccatcctgcg ccgcgaacgg gaacgcggcc
     3901 gagtcgagct cggtcctact ccggccgcgc aaccggcctg atcgcgaagc ggtcggggat
     3961 tgatcgcgag ccggccgctg ttaccctgcg cgacggtgct cgcttttcga ctctgcttgg
     4021 cggctctgct cgtttttgct gcgttcaccg gctcggtttg tctgcgcgcg caactgaccg
     4081 aggaggaaac acccgcgcac tcgttcaagt cccggcctac accgacgcca accccaaagc
     4141 ccaaaacaaa gaagaagaaa aaggtctccc cgacgccaac gccaacgccc aagccatcca
     4201 aaacacccga agcaagcgtg actcccacgg caacaccgac gaaaacgccg aaacccaaag
     4261 cgaaatcaac ggcaaaaaaa tcgcccgcga aagtcggcgg tgcggcgaag aaaaagaaaa
     4321 aaacaacctc tcctttgcca ggcggttcgc tgacagaaaa ggctcgttcg ccgcggccgt
     4381 cggaaacacc cgagattcga tctgcgttgc cagtggtttc accgccgccg tcatcattgc
     4441 cgcccgtcgc gccgccagtg gcaagatcga caactccatt tgccgcgccg gcggaagagg
     4501 agccggaact tacgattaag aaatcagact acgaacagcg gtcgttttgg gcgcgactat
     4561 tcggcttcgg ccggcggcgc agcggctacc gctatttgac gcgttcggtc cgcgccgcga
     4621 tcgaccgcgc accggtgatg cgcggccgct ggcagtttat tgccgtgcat aacagcggga
     4681 cgcgccaggg gaacgccgcc gtgttcgatt actatcaccg gcgcgtgcgg cggatgccga
     4741 acggcctcgc gtatcatttt gtgatcggta acggcacctc gactggcaac ggcgagatcg
     4801 aagtgggcga ccgctggcga cgtcagatca acggcggcca tgtgcacagt gattacctca
     4861 acagcatcgc gctcggcatt tgcctggtcg gcgatttcaa ccgcgatcaa ccgacgcgcg
     4921 cgcagctcga ttcctgcgaa gaactgatcc gttacctgcg ccagcgctgc ggcaagatcg
     4981 acaatcacta cccgattgtg aaaccgcacc gcgagctcaa tccgccgcga tggccaactg
     5041 attgtcccgg cgacgctttt ccatatacat ggtttcgccg cttccaataa tcattggcgc
     5101 tcggccacgg tgagattgct cttgtggaag tcccattggt agccgaaccc gatgtcgaac
     5161 ggcgccggat tactcacctg gtaaagctcc gccagcttcg gctgagaata ttttttgaat
     5221 agctcgatcg gaccgatgta ggtcccgaag aagcgcaggt tccatttgtt gcggtcgaag
     5281 tagctgatcg ggataccaga atcgtcctgc acgacgatgt tgctgtggtc gagaatgaaa
     5341 ttgcggacgc gggcgaatcc ttcctcgaac atgagatagg atgacgattt gaggaagctc
     5401 gcgcccacgc catgccggcc gcagaatttc atgaaggccg ggtttgcacc gatgccgcca
     5461 tcggaaatgt cggtcgtaaa gtaataaacc gtctgttccc gcccggaagc gttatcggtg
     5521 tagtcgatcc gcaccccggg cgtgccgccc gcgccgggag cactttcatg ctcgttgccg
     5581 gccgaatcga gcctaacgaa cgtgactgcg cggatcgtct tgtctccgcg cgccaaaaaa
     5641 acatacagga tcgggagcgt gccgttgagc tgctgttgct gcaaatcgac cttcatctcc
     5701 tgggtgatga aataggtcgt ggtaagcgac gccttcatcg cgttctccag gttttgcagc
     5761 gcccaaccga gatcggcgac gcgcaacgga tcgggcgcgg gtccgagcgc ctccttgccg
     5821 cacatgatat aaacggacgc gttcgggaag aactggtcga cgtaaagaaa atccggcccg
     5881 ctgaacatgt agtaggcgac cggcagtggt tccaatgatt ccggcagata ctgtctctgc
     5941 cacacgtgca gcttagccag tttgccgcgg ctcagcttcg agaacgcgtg ctcaaaataa
     6001 ctggcgtgcg cctgccaggc gggctgattc gtgagcgggt agagcggcga atctttcggg
     6061 agaggcatgc cggcgagaaa ccgcgctgtg tcattcggat cgggcacagg tatttgctgt
     6121 gcgcgcagcg ccagggtgcc gatcatcgat aaaaggcaga ttgagaatcg cgagatcgac
     6181 ctgcttcgcg cccgaagcgc agccagtttt ccgacgtggc tggctcgccc agctccttca
     6241 gattgtgtca gcgtttgcat aatctcacgt gcttagaagg agctgctggc aacatattca
     6301 ggcagtcggc cgtgttttct ttccggccgc aaaatacaga agcgttccga gcaggattgt
     6361 ggccaaacca gccgccgatt cccccgggtg cgagcgcaac aaataaacca tcatccaaat
     6421 ggtgacggcc gcgaaaacca ccggtggcaa tggatacagc cgcacgcgat atggccgcgg
     6481 cagatccgga ctcgtgacgc gcagcacgat caccccaacg accgtgagca gtgagcagag
     6541 cagcagactg aactggatgt aaatgacgat ctgctggaaa ttttttgcga agaaaagatt
     6601 cacgatcagc agttgcacca acgcggcgtt agtcggcaca ccgtgacgat ttttgcggcc
     6661 gagcaaccgc aacagccaat gatcttcgcc cattgccatc gtaacgcgcg gccccagcca
     6721 ggtcatggag ctgatcgccg agaccagacc cagacagatg acgccgccca caatgcgtcc
     6781 gccattcagc ccgaaaatgt gtttccccgc gatcagggcg acctcgagct gaccgctgag
     6841 ttcctcgcgg ggactgcaga gcaggaagac gccgttcagc agcacgtaga gaaccatcac
     6901 gacgctggtg ccgatcagga gcgcgcgcgg cacgtttttt tccggccttg tgatctcgcc
     6961 cgtaatgtaa gtcgcggcgt tccacccgga atatgagtac atcacgtaga cgagagcgac
     7021 ggcgaaggcg ccgcttaaga tcgacatcgt gtcgccggtc tgtggcagaa agtcgatcgg
     7081 ttgcttgggt tcgaccagta aacccgcgcc gatgagggcg ccgatcaaaa gcactttgac
     7141 caacgtccac aggttctgaa acacgctccc gacgcgcagg ttgccgagat ggaagagggc
     7201 cacgatccac accacagcga aggaaaagac gagcggagca ccgacgccaa aaacgccgtg
     7261 gaaatacttg ccaaaagcga ttgccgtcag cgcaatcgga gcagcgaatc cgaccgtcgc
     7321 cgaaacgatc ccagccatga aaccaagcgc cggatgatag atccgggaga gaaaatgata
     7381 ttcgcccccg gagcgcggta acgcggcgct gagctcaccg taacagagcg cgccgcaaag
     7441 cgcagcgatc ccgccgatga tccacaacat gagcagcgcg aaacccgatc ggatatccgc
     7501 cagttgaaaa ccgaggctgg tgaacacgcc tgtgccgatg atgttcgcga tcacaattcc
     7561 gcaagcagtc aaaaaaccga tcgtccgccg cggttcacgt ttttgcgcag tggcgagatc
     7621 ggtgacccgg ctcatggcaa gatcgacaat ctcgaaatta cccgcgctcg cgaatagttt
     7681 tgcgctgccg tccgctaccg aaaatcgaac gattgagttt gtcagaaccg gcggctggcc
     7741 tcagtttgga gggcgatgaa ttctcagctc aatctttcgg tcgaaccggc aacgcagctg
     7801 gcggtttttc tcgccaaccg gccgggtgcg ctgacgcgcg tctgcgcggc gctggctcag
     7861 gccggcatca acgtccacgc gctcaccacc tccgacaccg tcgatcactc ggtcgtgcgc
     7921 atggtcgtga gcgatccaac caaagcgctc atgttgctgg gcgaagctgg tgcgctcgcg
     7981 ctcgaagccg atgtgctcat gatcgaaggc gaaaaccaac ccggcgtgct ggaaagaatc
     8041 gccgcccggc tggccgaggc cagggtgaac atcgagtacc tttatctcgc cagcagcccg
     8101 aaggcggagc gcggcttgtt agtcttgcgt ccctctgatg ttgagaaggc gcagcgcgtt
     8161 ttgaaagacc tgtaagcaac gaagcctgcg gctggcaatc agccggcccc gaagatttcg
     8221 cagaggaaac gttatgacgc cgatcaaagc atggccgggc cgaccatatc cactgggcgc
     8281 gacctggctc ggcaacggcg tcaatttcgc gctcttctcg caacgggcga cggccgtcga
     8341 gctttgcctg ttcgacagcc tcgacgcgac cgaagaaaac attcgtgtcc cggtgatcga
     8401 acgcacgaat tatatctggc atgtcttttt gcccgatgcc cggccgggac aactgtacgg
     8461 ctttcgcgtt gccggatcgt acgagccgga gcgcggcctg cgtttcaaca gctcgaagtt
     8521 gctcctcgat ccgtatgcca aagccatcgc gggcgagata aattgggcgg atgagatgtt
     8581 cggctacgtg gtcggcgacg aactggaaga ccttagctgc gattttcggg a
//
LOCUS       DASTDG010000025         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_885477, whole genome shotgun sequence.
ACCESSION   DASTDG010000025 DASTDG010000000
VERSION     DASTDG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885477"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFO30_00930"
     CDS             <1..620
                     /locus_tag="VFO30_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9417879.1"
                     /translation="TNVPGDFVEAPSQMLQNWVWDKKVLDTFAADYRDVSKKIPGEIV
                     EKMNDAKRATAGVFYRRQFAFATLDLALHDEHPQNAPYDCIAISNPILERVFLPIDPS
                     TTFVTYFGHLNGYDAGYYGYAWADAIAADMATVFEKAKKGYLDKNAGMKLRHEIYEVG
                     DSRDVTVSIEKFLGRKQSVEPFLKKIGVTGEDKKKKPAGPSQEGK"
     gene            686..>2211
                     /locus_tag="VFO30_00935"
     CDS             686..>2211
                     /locus_tag="VFO30_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9417880.1"
                     /translation="MKTTLLILAISAMSIFAAELDDKLKPGLDAITPDGMLAHIKTLA
                     SDEFEGRAPGSKGEELSIKYISDQLKAIGLKLGNPDGSYFQEVPLAGITSDPRMTFVA
                     AGKPAMELKFPDDFVASSARLQNEIKIDNSDIVFVGYGVVAPEYGWDDYKDVDLRGKT
                     ILMLIGDPPVPDPNDPSKLDEKMFKGRAMTYYGRWTYKYEIAAQKGAAAAVIIHETEP
                     AAYPWQVVRSSWSKENFELDTPDKNMRAVQARSWITVDVAKKLLADCGQDFDALKKSA
                     VMNNFRPVALNAKAKIDIKQTLRAFKSRNVIGKLDGSDPKLKDECLLYTAHWDHLGRH
                     PELQGDQIFNGAVDNGSGCAAVLQLAAAFTKLNPPPKRTVLFMCTTAEEAGLLGAKWY
                     VEHPLYPLEKTLADINIDSMNVWGKARDVEDTSYGFSTLDDMLADAAKRQERVAIPNA
                     RPEKGSIYRADNFEFSKVGLPSLYIGKGEHLLSRAENAPLRSDEFDLKDYHQVTDEIK
                     P"
BASE COUNT          539 a          617 c          631 g          424 t
ORIGIN      
        1 gcacgaatgt ccccggcgat ttcgtcgaag cgccatcgca gatgctgcag aactgggtct
       61 gggacaagaa agtgctcgac acgttcgcgg ccgattatcg cgatgtgtca aaaaagattc
      121 cgggtgagat cgtcgagaaa atgaacgacg ccaagcgcgc gaccgccggc gttttttacc
      181 gacgacaatt cgcgtttgcg acgctcgacc tcgccttgca cgacgagcat ccgcagaacg
      241 cgccctacga ttgcatcgcc atttccaatc caattctcga gcgcgtcttt ctaccgatcg
      301 acccgagcac tacgtttgtg acttacttcg ggcatctcaa cggctacgac gccggttact
      361 acggctacgc atgggcagac gcgatcgccg cggacatggc caccgttttt gagaaagcga
      421 aaaagggcta cctcgataaa aatgcgggca tgaagctgcg gcacgaaatt tacgaggtcg
      481 gcgactcgcg cgacgtgact gtgtcgatcg aaaaattcct cggacgcaaa cagtcagtcg
      541 agccgttcct gaaaaagatc ggcgtgaccg gcgaggataa gaagaaaaaa cctgccggac
      601 cgtcgcagga aggaaaatag tggcgcatgt gtcccgcttg cgcggtttga aattcgcaag
      661 ccagaggctt gcgctacatt gcagcatgaa aacgacgctt ctgattcttg cgatcagtgc
      721 gatgtcgatc ttcgcggccg agctggacga caaacttaag ccgggcctcg acgcgattac
      781 ccccgacggc atgcttgcgc acatcaaaac gctggcttcc gatgaatttg agggccgcgc
      841 acccggaagc aaaggcgaag agctgtcgat caaatacatt tccgatcagt tgaaagcgat
      901 cggacttaag ctcggaaatc ctgacggtag ttactttcag gaagtgccgc ttgccggaat
      961 cacaagcgat ccgcgcatga ccttcgtggc cgccggcaaa ccggcgatgg agctgaagtt
     1021 tcccgatgat ttcgtcgcgt catcggcacg gttgcagaac gaaatcaaga tcgacaactc
     1081 cgatatcgta ttcgtcggct atggcgtcgt cgcgccggag tacggctggg atgattacaa
     1141 agatgtcgat cttcgcggca aaacgattct gatgttgatc ggcgatcctc cagtgccgga
     1201 tccgaacgac ccgtcgaagc tcgatgagaa aatgttcaaa gggcgcgcga tgacttatta
     1261 cggccgctgg acttacaaat acgaaatcgc cgcccagaaa ggcgcggccg cggcggtgat
     1321 catccatgag accgagccgg cggcctatcc ctggcaagtc gttaggtcga gctggtcgaa
     1381 agaaaatttc gagctcgata ctccagacaa gaacatgcgc gcggtccagg cacgctcgtg
     1441 gatcacagtc gatgtcgcga agaagctgct cgcggattgc ggccaggatt tcgatgcgct
     1501 caagaaatct gcggtcatga acaattttcg gcccgtggcg ttgaacgcga aggcgaagat
     1561 cgacatcaag caaacattgc gcgcattcaa gtcgcgcaat gtcatcggca agctcgacgg
     1621 cagcgatcca aagctgaagg acgagtgcct tctttacacc gcgcactggg accatctcgg
     1681 ccgccatccg gagttgcaag gcgaccagat ttttaatggc gcagtcgaca acggctccgg
     1741 ctgtgcggcg gtgctgcagc tggcggcggc gttcacaaaa ctgaatccgc caccgaaacg
     1801 aaccgtgctt ttcatgtgca ccacggccga ggaagccggt ttgttaggcg cgaaatggta
     1861 tgtcgagcac ccgctctatc cgctcgagaa aacgctcgcc gacatcaaca tcgacagcat
     1921 gaatgtctgg ggcaaagcgc gcgatgtcga agacacgagc tacggcttct ccacattgga
     1981 cgacatgctt gccgacgccg cgaagcgcca ggagcgggtt gccattccca atgcgcggcc
     2041 ggagaaagga agtatttatc gcgccgacaa tttcgaattc agcaaagtcg ggctgccgtc
     2101 gctttacatc ggcaagggcg aacatctcct gtcgcgtgcg gaaaacgcgc cgcttcgctc
     2161 ggacgaattc gacctgaaag attatcacca ggtcaccgat gaaataaaac c
//
LOCUS       DASTDG010000026         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1421692, whole genome shotgun sequence.
ACCESSION   DASTDG010000026 DASTDG010000000
VERSION     DASTDG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421692"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFO30_00940"
     CDS             complement(<1..564)
                     /locus_tag="VFO30_00940"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9417881.1"
                     /translation="MRSADRINFLITGGAGFIGSNLTLALQEKFADARLTVIDDFRSG
                     FFKNLRGYRGDFVAEDLAKLNWQEQFGDQKFDTIFHLASITDTTLHDQFVQMHDNVES
                     FRRILQFAQPTKTRIIYASSAATYGAAEEASVESNGAAPANVYAFSKAIMDNIATRAA
                     SDNPDWIIIGLRYFNVYGPREAHKGVPA"
     regulatory      700..897
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1404..3167
                     /locus_tag="VFO30_00945"
     CDS             1404..3167
                     /locus_tag="VFO30_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9417882.1"
                     /translation="MSATRTDIPLDQSPASASVIDSQELEQKQIERVSDALREVPGLS
                     VVQTGSPGQLTSVFTRGLRSEHTQVLLDGVPINQGLQGAFNFADLTTDDIDRIEIVRG
                     PQSTIYGPRALAGVIQIFTKQGEGEPGITLTGEGGSYDTFREALQSDGKIDNFDYSLG
                     ASRLDTDNARPNNQYRNTASIANVGWSPNEQLRLSSLFTYSISDIGLPNTIFNPKPRD
                     NFFTERWLIAPHVDLHVGDWWEHKFIFSYDHERQINDPNEDGFVGPTRALFERTQVDY
                     QNDLRPTSWLTVTSGFFYSRVNAGQERPFVSQAFGPQPTFVSDHTQEVAGFLEGTAKI
                     DNFIFVAGGRFDHFNQFGDVWTYRVAGSYKIDKTDTTLHSSVATGFSPPSSQDKIFGM
                     NFGLRPERDLGWDIGVEQRLWENRVTIGATYFHNDLSNVIGFNGLFQTLNLGAAETQG
                     VETELRAQPIADLIFTVTYTYLEAEKTSSADISQPQGARLPRRPRNEVYVSASYLWWK
                     KLRTTAEAKFVNAREELNFAGPNFDIEDYSFVNVAAEYEINSHLSIFGRIDNLMNEHY
                     AEVFGFPALGRAAYGGVKLRF"
     gene            3213..>3610
                     /locus_tag="VFO30_00950"
     CDS             3213..>3610
                     /locus_tag="VFO30_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417883.1"
                     /translation="MSPLVWLVIGMLAGGVTATVILLLARYSKRGDGVQDDLVRRLEI
                     LDRGLRDEFSRNREEGAAAAKGQREELGKSLETVRTIVDLRLKELQQDNAQQIERMRA
                     TVDEKLQGTLEKRLGESFKLVSERLEQVHRG"
BASE COUNT          794 a         1001 c         1075 g          740 t
ORIGIN      
        1 ggcagggacg cctttgtgcg cctcacgcgg gccgtaaacg ttgaaatagc gcagtccgat
       61 gatgatccag tccgggttat cgctcgctgc gcgcgtcgcg atgttgtcca tgatcgcctt
      121 agaaaatgcg tagacgttcg ccggcgcagc gccgttcgat tccacgcttg cttcttccgc
      181 ggcgccgtac gtcgcggcgg acgaggcgta gatgatccgc gttttcgtgg gctgcgcgaa
      241 ctgcaaaatc cggcggaaac tttcgacgtt atcatgcatt tggacaaact ggtcgtgcaa
      301 agtcgtgtca gtgatggagg cgagatggaa aatcgtgtcg aacttctggt cgccgaattg
      361 ttcctgccaa ttgagcttcg ccaaatcctc cgcgacaaaa tcgccccggt agccgcgcag
      421 gtttttgaaa aagcccgagc ggaaatcgtc gatcaccgtc agccgcgcgt cggcaaattt
      481 ttcctgcaac gcgagtgtga gatttgagcc gatgaaaccg gccccaccgg tgatgaggaa
      541 gttgatacga tcagccgaac gcacagcgag cttagttcag tcactaaata aatcttgagc
      601 ctcgaatcgc aagccgctac ttcttcgcca cggcgaatcc gtcctgtggc ggatgcgcgc
      661 agttctttgc gagcttaagg aaagtggaac ggcgcgagaa atcccgtgtg aatcgggagc
      721 agttgcgctg ctgtaactgg atacgacttc ccagggccaa tcgttgtggt ggcaggcgta
      781 tcgcctgcga ctctgcggtt gtgcagccga gacggctgcc gccacagaaa cggtgaaggc
      841 gggaagaagg atggagatta ctcggtagtt gatggttgag agttgatagt cgggagatgc
      901 cgggcgtctg cctctatcaa cgatcaactt aaaactatca gctattccga acgccaggag
      961 ccagaatatt cgaccgttcc acctcattcg tgcgcgtgag tgcgcgttac gaaaaaactt
     1021 cttcgcaaaa agaagaatga gagctgggcg atgcgtcatt gtagcggcga tctctgatcg
     1081 ccgaagggcg acgacggtcg aagaccgccg ctacaggggc aaacaagtcc gactctcgga
     1141 gttggaggta acttgttatg cagattcgcc cagtagcttt tgttttacgt ctttcacgcc
     1201 gcggttgggg tcgcgccgct gtttaccctc accgcctcgc cgcggcgagt gcgcctctcc
     1261 ctcagggaga ggcaatggtg agggcaatcg cagccgtagc cgttgcgttt gtctgttttt
     1321 tcaagccgcc ccttctactc gcgcaagacg ctgtaagttc gacatcggac gtcgagagcg
     1381 aagccgaagc ggagccggta attgtgtctg cgacccggac cgacataccg ctcgatcaat
     1441 cacccgccag cgccagcgtt atcgattcgc aggagctcga gcaaaaacag atcgagcgcg
     1501 tgagcgacgc gttgcgggaa gtgccggggc tttcggtcgt gcaaacgggc agccctggtc
     1561 agctcacttc cgtattcacg cgcggattgc gcagtgaaca tacacaggtg ttgctagacg
     1621 gcgtgccgat taaccaaggt ttgcagggcg cgttcaactt tgccgacctg actaccgacg
     1681 acatcgaccg aatcgaaatc gtgcgcgggc cgcaaagcac tatttatggt ccgcgggcgc
     1741 ttgccggggt gattcagatt ttcacgaagc aaggagaagg tgaaccgggg atcaccctga
     1801 ctggcgaagg cggctcgtac gacaccttcc gcgaagcgct gcaaagcgat ggcaagatcg
     1861 acaacttcga ttactcgcta ggcgcaagcc gtctcgacac cgataatgcc cgtccaaaca
     1921 atcagtaccg aaacaccgcg agcatcgcga acgtgggctg gtcgccgaat gaacagttgc
     1981 gcctgagcag tctgttcacg tattcgatca gcgacatcgg cctgccgaac acgatcttca
     2041 acccgaaacc gcgcgacaat tttttcacgg aacgctggct catcgcgcca catgtcgacc
     2101 ttcacgtcgg cgactggtgg gaacacaagt tcatcttcag ctacgaccac gaacggcaga
     2161 tcaatgatcc gaacgaagac ggttttgtcg ggccgacgcg ggccctgttc gagcgtaccc
     2221 aagtcgatta tcagaatgat ctgaggccaa catcgtggct gacggtcacg tcgggatttt
     2281 tttatagccg cgtgaacgcc ggccaggagc gtccgtttgt atcgcaagct ttcgggccgc
     2341 agcccacctt tgtcagcgat cacacgcaag aggtcgccgg ctttcttgaa ggcaccgcta
     2401 agatcgacaa tttcattttc gtcgcgggcg ggcgcttcga ccacttcaac caattcggcg
     2461 acgtctggac ctatcgcgtc gctggcagtt acaagatcga caaaacagac accacgctgc
     2521 attcgagtgt cgcgaccggc ttcagcccgc cgagcagcca ggataaaatt ttcgggatga
     2581 attttggtct gcgtccggaa cgcgatctcg gctgggacat cggcgtggag caacggttgt
     2641 gggagaaccg cgtcaccatc ggcgcgactt atttccacaa cgacttgtcg aacgtgatcg
     2701 gcttcaatgg actgttccag acgctgaacc tcggcgcggc tgagacgcag ggagtcgaga
     2761 ccgagctgcg cgcgcaaccg attgccgatc tcatcttcac cgtgacctac acatatctgg
     2821 aagcggagaa gacttcatcc gccgacattt cgcagccgca aggcgcgcgt ttgccgcgcc
     2881 gcccgcgcaa cgaggtttac gtttcggctt cttacttgtg gtggaaaaaa ttgcgcacga
     2941 cggcggaggc aaagttcgtc aacgcccgcg aggaattgaa tttcgccggg ccgaacttcg
     3001 acatcgaaga ctacagcttc gtaaacgtgg cggccgaata cgagatcaac tcgcacttgt
     3061 cgatctttgg ccgcatcgac aacctgatga acgaacatta cgccgaagtg ttcggttttc
     3121 cggcgcttgg acgggcggct tacggcggcg tgaagctgcg gttttagcaa cccttgtcat
     3181 tccgagcgaa gtcgaggaat ctctcaacgg aaatgagtcc gctggtttgg ctcgtgattg
     3241 gaatgcttgc tggtggagtt accgccaccg tgatacttct gctcgctcgc tacagcaagc
     3301 gcggtgatgg cgtgcaggac gatctcgtgc gacgtctcga aatcctcgac cgcggactgc
     3361 gcgatgagtt ttcgcgcaac cgcgaagagg gcgcggcggc ggcgaagggc cagcgcgaag
     3421 aactcggtaa atctttggag accgtccgca cgattgtcga tcttcggttg aaagagctgc
     3481 agcaggacaa cgcgcaacaa atcgagagaa tgcgcgcgac ggtcgacgag aagctgcaag
     3541 gaacgctgga gaaacggctc ggcgaatcat tcaagctggt gagtgaacgg ttggagcaag
     3601 tgcaccgcgg
//
LOCUS       DASTDG010000027         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_667675, whole genome shotgun sequence.
ACCESSION   DASTDG010000027 DASTDG010000000
VERSION     DASTDG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667675"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..217
                     /locus_tag="VFO30_00955"
     CDS             65..217
                     /locus_tag="VFO30_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417884.1"
                     /translation="MTITRNVGMLLLAIYLILIGITVLFAGIAIPPVLTGVIALVAGI
                     FILIGR"
     gene            278..1018
                     /locus_tag="VFO30_00960"
     CDS             278..1018
                     /locus_tag="VFO30_00960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9417885.1"
                     /translation="MRLSRKIEKLLSPDVFLSGWEHLCRWTHPISARDILARIDGEQL
                     ARLRERYPARANARQINAYENAAYWIGVNVRRVQDLWLDRTPPLRILDLGCGPGYFLY
                     ISQLFGHSGIGLDTPDEPLFEGLTQLLNIQRLIARIERQVPLPDLGEEFDLITAHRVC
                     FHRISRVSEHEWREWSPADWKFFIDDVRARFLKPHGRMFLELNPRYDGSSAITPDIRA
                     AFLAEGARISRTKVLFAAEPSNRPRFKL"
     gene            complement(1065..1859)
                     /locus_tag="VFO30_00965"
     CDS             complement(1065..1859)
                     /locus_tag="VFO30_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9417886.1"
                     /translation="MFRPKQRKIDKLNFIIAGAQKSGTTALHYFLQKHPQITLGDQQE
                     MHFFDTDEFFARQPVDYEALHRHFPRFARSIRHRTDSLRRTIAGECTPHYLHYKPAME
                     RIWNYNPAIKLIILLRNPVDRAFAHWNMQRFKRRDPLDFLDAVQAEKDRVVENSPLQA
                     RRFSYVARGFYSEQLARVFKFFPREQVKLIKFEHFRDHNREALNSVFEFLRVKPLPRI
                     RSKDRNVVPYERAMTIDERKHLYEIFADEILRLERTLGWDCSDWKL"
BASE COUNT          410 a          601 c          607 g          503 t
ORIGIN      
        1 tgccgcctcg tgagcgccac attttccgcg tgcaaagcga ggccgtttgc ctcagaacga
       61 ccgcatgacc atcactcgaa atgttggaat gctgttgctg gcgatttatt taattctcat
      121 tgggatcacc gtgctgttcg cagggatcgc gattccgcct gtgctgaccg gcgtgattgc
      181 gctcgtcgct ggcatcttca ttcttatcgg tcgttaattg tcgatcttga gtcgtggcgc
      241 cacttgtctg gcgcgtgctt agccgcatcg ttgaccgatg cgattgtcgc gcaagatcga
      301 gaagttgttg tcgcctgatg tttttctcag cggctgggag cacctgtgtc gctggacaca
      361 tccgatcagc gcccgcgaca ttctcgctag aattgatggc gagcaattag ctcggctgcg
      421 cgagcgttac cctgcacgcg cgaacgcacg ccaaatcaat gcctacgaaa acgccgccta
      481 ctggattggg gtcaatgtgc gccgcgtgca agatctctgg cttgatcgca cgccgccgtt
      541 gcgcattctc gatctcggct gcggccccgg ttacttcctt tatatctctc aattattcgg
      601 ccacagcggc atcggactcg acacaccgga tgagccgttg ttcgaggggc tgacgcagct
      661 gctgaacatt cagcgcctga tcgcgcgcat cgaacgccag gttcctctgc ccgatctggg
      721 agaagaattc gacttaatta ccgcgcaccg cgtttgcttc caccgcattt cgcgcgtgag
      781 tgaacacgaa tggagggaat ggagcccggc cgactggaaa tttttcatcg acgatgtccg
      841 tgcgcgtttt ctgaaaccgc acggccgaat gtttctcgag ttgaacccgc gctacgacgg
      901 ctcgtccgcg atcacgccgg atattcgcgc ggcatttctc gccgagggag cgcggatttc
      961 ccgcacgaag gtgctatttg ccgccgagcc gagcaatcgg ccgcgcttta aattgtaggg
     1021 caggcgcccc gcctgccaca acctgccgct gcggacaccg gccgctacag tttccagtct
     1081 gagcagtccc agccgagcgt tcgttccaat cgcaagatct cgtcggcaaa aatttcatag
     1141 agatgctttc gctcgtcgat tgtcatggcg cgctcatacg gaacaacatt ccggtcctta
     1201 cttcgaatgc gcggcagcgg ttttacgcga agaaattcaa aaactgagtt cagggcctcg
     1261 cgattgtggt cgcggaagtg ctcgaacttg atgagcttga cctgctcacg tggaaaaaac
     1321 ttaaatacgc gcgcgagctg ctcggagtag aaaccgcgcg cgacgtaaga aaagcgccgc
     1381 gcctgcagcg gggagttttc cacgacgcga tccttttcgg cttgcactgc gtccagaaaa
     1441 tcgagcgggt cgcggcgctt gaagcgctgc atgttccagt gggcaaaggc acgatccacc
     1501 gggtttcgca gcaggatgat caatttgatt gccggattgt aattccaaat ccgctccatc
     1561 gctggtttgt aatgtagata gtgcggcgtg cattcgccgg cgattgttcg ccgcagcgaa
     1621 tccgtcctgt ggcggatcga tcttgcgaac cgcggaaaat gccgatgcag cgcttcgtag
     1681 tcgaccggct ggcgcgcgaa aaattcgtcg gtgtcgaaaa aatgcatctc ctgctgatcg
     1741 cccaaagtga tctgcggatg tttttgcagg aaataatgca acgcggtcgt gccggacttc
     1801 tgcgcgccgg caatgatgaa attcaacttg tcgatcttgc gctgcttcgg gcgaaacatg
     1861 gaagctctgt agccacgggc ctgtgggcca ttgtttgtga cggcagcatc gtcgcacgac
     1921 taagctgtgc gggcgagctt cgcgtgtgcg tgcacgctcg catagcaatt gtcgatcttg
     1981 cggggacgtt ttagctttgc gcgatgatcc tgcgcatttt catttctttc gtcaccatgt
     2041 ctgccgccgc gtctatgctg cttgccgatg aaggcatgtg gttgttcaac aatccgccgc
     2101 tccagcgact gaaagagcgc t
//
LOCUS       DASTDG010000028        17772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_449103, whole genome shotgun sequence.
ACCESSION   DASTDG010000028 DASTDG010000000
VERSION     DASTDG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449103"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..688)
                     /locus_tag="VFO30_00970"
     CDS             complement(86..688)
                     /locus_tag="VFO30_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9417887.1"
                     /translation="MKRSSILLWVIGAIIAAIAIGVAFYFDESVRDFLAQHQNRAVKI
                     FMLNVSRFGDWPEHFGLGIVLLAIAWWRGQKRWTRIFLSMLIALALAGLVGRAIKIST
                     GRARPSVKAEELWSGPRLSSKFHAFPSGHVLASSAFFAVLTFARWRIGLPCLAAIPAL
                     IGFSRMYAGAHYLSDVVCAAVIGLLSGFVVWRIAVQKSGG"
     gene            735..2243
                     /locus_tag="VFO30_00975"
     CDS             735..2243
                     /locus_tag="VFO30_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9417888.1"
                     /translation="MKSATRDYALLFFGCLLFHTIGTWSLPLIDRDEPRFAEASREML
                     ERGDYIVPHFNNQLRLDKPPLAYWAQVASFEVFGENDFAARFPSAVAAALVALSIFAW
                     GNRIAGERIGWWAAVIFTLSLQTFIHAKAAVADMWLVLFVTIAHWSGYELLRDSLTDD
                     GGKRPTLNSDKSRAGAQRPTWNSEHLRWWLAFYAALGLGFLAKGPIGWTPLLTVASIK
                     VFGPDLALAKRFKFLRGTLLMLAIVALWGIPALIRTHGEFLAIGIGRHVIGRSFAAME
                     GHGANSLGIYLLLLPFYFITVFVSFFPWSIKLPRLARRLWNDRDKIDNYLLAGVAIVF
                     LIFSLISTKLPHYILPAFPLLSLLLARRLVDEQKFFKAAAIAAVCIYLVVAFVVPPLA
                     ARCFPAKQLYNAARDSLRRDMEFGAVGFEEPSLVWYFRTRVNGWMSSLRPKDATEFMQ
                     RPGARFIVLPTKLAHELFPIPSRAWKMFSVSGFNIAKGAHVELTLVLKPQQL"
     gene            2356..2967
                     /gene="ruvA"
                     /locus_tag="VFO30_00980"
     CDS             2356..2967
                     /gene="ruvA"
                     /locus_tag="VFO30_00980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9417889.1"
                     /translation="MITFLDGRLVSALPTQAIVDVAGVGYEVLIPLSSYDRLPQVGQT
                     IHILTHLAVREDAHVLYGFMTVAERDLFRLLVNNVSGIGPKLALAVLSGMSVTNFKAA
                     VVNSDVAALAKISGLGKKTAERIILELKDKIGVAAAWEAASAAHAPTPEQQQASEAVL
                     ALIALGYKQIEADKAVRDLQQTGEAKSAEELLKLALKKIATGR"
     gene            3020..4345
                     /locus_tag="VFO30_00985"
     CDS             3020..4345
                     /locus_tag="VFO30_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417890.1"
                     /translation="MSDVRTNMIAVGEDRLATIRDAFPKAGLFADKDWLVSPDPFPIE
                     SKFLAELEQLGHRLYVFQRACNQLYQLSIKGKQPAWVARYLDAGKPPELIEFSRRKQI
                     RDDVPRVIRPDLILTDEGYIIAEIDSVPGGIGLTAWFNETYSVFDPHIIGGSDEMLAG
                     FETVLPNGGDIVISEESATYRPEMEWLAGRLQDRQSACRVVAAGNYEPQDGRAVYRFF
                     ELFDLPNIPKIDNLLRANAQGRVTITPPVKPYLEEKMWWALFWLRPLREFWRRELGEK
                     YFIKLQEAIPYSWLMDPTPLPQHAVIPRLEIHEWREAAGFSQKHRELLLKVSGFSPLG
                     WGSRGVWFGSDLPHAEWERKIDNALATFESSPTIMQHFHKGRLFSHRYWDAATSELKT
                     MNGRVRLCPYYFIDAGRVKLCGALATIAPADKKFIHGMQDAILVPSARQ"
     gene            complement(4442..4993)
                     /gene="thpR"
                     /locus_tag="VFO30_00990"
     CDS             complement(4442..4993)
                     /gene="thpR"
                     /locus_tag="VFO30_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9417891.1"
                     /translation="MSKRLFVAISLPDSTRDALVDLNPRMRGVGWSDRDQMHLTLGFF
                     GHVPEDVDLALREKLRTIQFGAFFLPITCLGTFPPKGAPKIIWVGVGKGHPHLFQIHK
                     RVQDAALAVGLQPDLRPWHPHITLGRCRDVSAHTLRKFLQTNADLDLGLIRVDAFHLY
                     SSQLTPAGPIHTHELTVRCSAGY"
     gene            complement(5063..6070)
                     /locus_tag="VFO30_00995"
     CDS             complement(5063..6070)
                     /locus_tag="VFO30_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9417892.1"
                     /translation="MRWFISVLILVFGGCIASRVYRSRFSFQDKVALISGGSRGLGLV
                     LARQICDEGGRVAILARDPDALARARSDLSARSGEALTLQCDLHDRGQIESAVQQTLE
                     RFGRIDILINNAGIIEVGPFEHMSREDFERCMGIHFWAPLELIMQVIPAMRRQRGGRI
                     VNISSIGGKIAVPHLAAYCASKFALTGLSDSLRAELARENIYVTTVAPGLMRTGSHVN
                     AKFKGKHDAEFAWFSVSAGAPLISMNAERAARKILAASRRGQPSLTLTFAARGAIVGN
                     ALFPNLTGYVMKLANRFLPGTTGDAGNESRRGGDVPRLAPEWVTHLADRAIEKNNESC
                     Q"
     gene            complement(6125..6589)
                     /gene="smpB"
                     /locus_tag="VFO30_01000"
     CDS             complement(6125..6589)
                     /gene="smpB"
                     /locus_tag="VFO30_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9417893.1"
                     /translation="MAAESILNRKALRDFHILERYEAGIALKGSEVKSVRAGKANIND
                     AFARVENGEAFLYNADIQPYEKASHEVPAPKRVRKLLLHRQEIDKLYGETQIGGRALV
                     VLKLYWKNGKLKAELGVAAGKTARDKRADLKKRAVERETAREVARFNRRHGT"
     gene            complement(6616..6933)
                     /locus_tag="VFO30_01005"
     CDS             complement(6616..6933)
                     /locus_tag="VFO30_01005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9417894.1"
                     /translation="MLAPQLTLVALAGESPTLLQFPNGTLRAVHSLRHMTTQLLQEAA
                     RKIPNQQLLINVVSKRVRQLGLGHRPMVEITPRMSLTDIALKEIIEDKLSYESLSGSA
                     DNA"
     gene            complement(7070..8506)
                     /locus_tag="VFO30_01010"
     CDS             complement(7070..8506)
                     /locus_tag="VFO30_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417895.1"
                     /translation="MNQPQSGENANVLVCALCTILIVSMIGGSVLLNCATRYNAASNQ
                     VRGWKEALHAAEGGGDIAYAQVRNTVMDPSHAFAGWTYTGGVYANTPVTFGSNLTTSS
                     TVDIFYYDAWNGNPWYRIRSRGTAPVRGLKRTGMDDRMLTGSRGDSLLRKVDFNYDHF
                     IAAYGPSGDGTSKAIIPVAQPQITRRIEQVCAPITPFEAAIKCIGTFYGLGDAAMIDS
                     FDSRNGAYYFAALSPNDPHYSDSRSGNVEIDTAVATVRGWIYGDVATNGGTIVRSQYI
                     SGTIDNNVPFSVERYYLPTNLPLPQPSPAAVTSTVIITPPAAGTEANPTLYLLSSLSG
                     QLTINSFGSQKTYVALHVTGDVSGSIDTKPLVKAKIFVDGNMDVKGRDIINESGIAAN
                     LQYYAISPTDPSTMQHININPPGNFAAVFYGPGADVSINGNPDLTGAVVCKTFYANGN
                     VSWHYDRELGLEGDAVDYRITSYVEDIR"
     gene            complement(8546..9175)
                     /locus_tag="VFO30_01015"
     CDS             complement(8546..9175)
                     /locus_tag="VFO30_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9417896.1"
                     /translation="MKIAHSTRNRLGFTLVETTVALGISSIVFVAAITASIGLQKSFN
                     AIDNFFATHMQQIRIIDYLSRDVKRGLIVTTSSDLQSVSITVPKYIIQAGDPEAVANP
                     SLIGTPRTPTVSYSRSGSQVNYGSSTSSVVYAISGQSILRTENGVVTTIASATDQLVP
                     QWTDVELANTEYTSTTVTFLPIFTSANATAERSGTTVCATAYLRNRRRG"
     gene            complement(9172..9666)
                     /locus_tag="VFO30_01020"
     CDS             complement(9172..9666)
                     /locus_tag="VFO30_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417897.1"
                     /translation="MKRNSLSTQAGTTMPEVLVAALLLAVFFGSIFELNAVCLRYIDA
                     SKESIAALQLVNDRAETLRNLAFSDLTNSSYVQNLLSSPANASEFAKKATEVVKISAY
                     PTASGVTQFTRSANGTVTADSVAADLGSTLVQVDVAISWMMSLGTRARSEQLTSIISN
                     GTKK"
     gene            9965..10038
                     /locus_tag="VFO30_01025"
     tRNA            9965..10038
                     /locus_tag="VFO30_01025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9999..10001,aa:Pro,seq:cgg)
     gene            10092..10793
                     /locus_tag="VFO30_01030"
     CDS             10092..10793
                     /locus_tag="VFO30_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417898.1"
                     /translation="MNQTEFSAIALSGFTVAFFHVAMPTHWLPFVLTARAQRWSRAKA
                     LAITALAASGHVVFTALLGLLVTVFGFALHERTAAWFPRVGGSVLVLFGLLHLYRQVL
                     GHVHGHSRVIGEDPRVHAAHGAFGHELEIERHDATHVPDHVAITSLLALLTFSPCEAF
                     VPFYISGLHYGWFGFWLLTAILSIATLTGMVALTWLTLVGIRQIALRQLERYELGVMG
                     ILLCAVGVLIIIFNP"
     gene            11093..12916
                     /locus_tag="VFO30_01035"
     CDS             11093..12916
                     /locus_tag="VFO30_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417899.1"
                     /translation="MRARNSYMGAVVLLSLCFALPCFSGNFVRPREALPTLSPPKLRT
                     GAIPQDDSFSAVAAKDLTLRVDGEHKAEALAHFVEGNAFEENGEMDKALHAYREVLNV
                     DPGQSELASHVAELLSRQSDFPQAIDVLKDAIAVNPNKAEPYLQLAFIYARYLKRSDQ
                     AIEYVNRAIALDPRDIDAYQRLFELLSAAGDDKKALQALERAAKVQTDNASFWARLGK
                     LYAAAIFEPGRNPKPTERARVNEIFKKAAENGKDDANILKDVADFYASSQQLQQAIPL
                     YLRVLELQPDDVNAREKLATGFVLTNQRAKAIEMLAQLIKEHPEKYEPYDLLAQIFED
                     DARSLERANRNQEAKLQYEQAAANYEQSLLIAPTHPNTYLRLAELLLGPCKDAQRAVK
                     LLSEARRRFPNAPELIYYMALAQREAKHPQEAVATFEEARHEAELEDSEMANARFYFN
                     YGATAEQAGLYEKAADLFRKSIAMDPANAADACNYLGYMWVQQNAHLDEAEAMIKRAL
                     QSEPDNGAYLDSLGWLEFRQGKFEQALKDLLRAAKSLTRDDPVVYEHIGDAYLQLKRM
                     PQALEAWQKALALDPENKTLAGKIESTKTMMSKSEPPNSGA"
     gene            12986..14344
                     /locus_tag="VFO30_01040"
     CDS             12986..14344
                     /locus_tag="VFO30_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9417900.1"
                     /translation="MRNYPTAWQRKLMWAALSAFFTVLLIAVAASAIWVVANLLSFLQ
                     PILIPVAIAVILAYLFDPLVTKLERGLLTRTKAILLIFAVAFLFLAGLIAWVAPTVSM
                     QSANFARELPNYTQKARDRIVDVIVRFNRTFAAPSRRGKTVSPSSGIVNLLLGTPSPQ
                     ASAAASATPVPPPAAVAPGASVTPAPVKLTSADRQRLQAYFDQLIPNLERNIPTLAAQ
                     LWDILKKSIGGFLGFTGFLLSLVLVPIYLFFLLKERPRIERRWKDYLPLRDSPLKNEI
                     AEVLSQINDYIIAYFRGQLLVCLVDGVLIGSALMLFGLNFAALIGMLVVILTMVPYIG
                     IVLCWVPAVLIAAFQWGDWWHPFWVTVIFVVIQNLEGMFYAPRIVGNSVGLHPMTVIV
                     SIFVWGLLIGGVLGPILAVPLTATLKVLLMRYVWAPRVREEEIAEIEQVPVVAESERA
                     KA"
     gene            14478..16361
                     /locus_tag="VFO30_01045"
     CDS             14478..16361
                     /locus_tag="VFO30_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417901.1"
                     /translation="MPRFIRHSPRVRLFAVIGIAAAIFVIAMFFLGSVAKAYNHWRQN
                     RLLQRANTLLNEAASKEIATATRKNDLEQAMRIGKEVLRLRPDSLSAYYVLAEAAEKS
                     NLEETVGWRAQIARLLPHDLDSQLNLASAALRFGQLDLARKALEKVPAVDRDRAPFHV
                     VAGWLARAEGNVSEQEHQFAAAVKKEPGNDLYQFNLAVLRIRSPDPDKRADARATLER
                     LSKTTGFRAGALRALLNDAVERSDFAAADSIAQQLQMIQQVTFNDYLLCLNFYRKLDD
                     KKFGALLQKVKAFAAHDPSEVALLMSWMNTNGLAAEVLEWLDKLPSEISTHPPVAITV
                     AEAFAAAKNWSRLKRWTRTGSWKDADYLRLAYQALAARQSRQSGGEAEFNSLWRSAER
                     EADEQPEYQAQLARLATKWNLTIEADALWSRVAVTPPLRREALNALYHIYRENNELAK
                     IYDVLQRLHQTSPNEPAITANLARLGMNLDQNTDQAHRLAREAYERAPEDTNCAVTYA
                     YSLYGMGRTSDAIEVLKKLPPEQLHDPHAAVYVAAMLLDENQPDAAGEYIQAAAHSRL
                     YVEEKSLLEEAKTKQAALLAAASLSPSPADSPAVLGSSPVAPTATPVPSPTASPLPSP
                     SPR"
     gene            complement(16370..17146)
                     /locus_tag="VFO30_01050"
     CDS             complement(16370..17146)
                     /locus_tag="VFO30_01050"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET9417902.1"
                     /translation="MPNGEKGSADFYRESMCVLERAGVPFLVGGAYAFAFYTGISRDT
                     KDFDLFLRPRDVDRALDAFQRAGYGAEKTYPHWLAKVRAGTDLIDLIFRAGNGLCGVD
                     DLWFARAERAELLRSAVQLCPPEEIVWMKAFIMERERYDGADVAHLFQSCAERIDWRH
                     LLDRFGPDWEVLLSHLVLFGYIYPNERGRIPKHVMEELVQRFPNEAKPLAERICRGTL
                     LSRAQYLIDVKERGFRDARLESRVQMGPAEISDWTKAIDE"
     gene            17282..>17772
                     /locus_tag="VFO30_01055"
     CDS             17282..>17772
                     /locus_tag="VFO30_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9417903.1"
                     /translation="MAQRKLRIAATADIHYGKHSRGALHDTFEEISRAADVLLLCGDL
                     TDYGLAEEAEQLVADLRAAVRIPTIAVLGNHDFESGESELVCKVLDGAGVNVLNGEAV
                     EIAGVGFAGVCGFAGGFGRRMLNAWGEPLIKQFVQEALDHAVRLEQALAKLRTDRRIA
                     VLH"
BASE COUNT         3762 a         5097 c         5102 g         3811 t
ORIGIN      
        1 ggttgattgc gccggtcttg aaaaccggaa ggccgaaagg cctcgtgagt tcgaatctca
       61 ccccctccgc cgattaactc tcgtctcaac cgccactctt ttggacggcg attctccaga
      121 cgacaaatcc acttagcagt ccgatgaccg cggcacaaac tacgtccgac aagtaatgcg
      181 cgccggcgta catccgcgag aacccgatga gcgccggaat cgccgccaga cacggcagac
      241 caattcgcca cctcgcgaaa gtgagcacgg caaaaaacgc agacgaagcg agaacatgac
      301 ccgacgggaa tgcgtgaaac tttgagctga gtcgcggccc actccaaagt tcctcggctt
      361 ttaccgacgg ccgcgctcgg ccagttgaga ttttgattgc gcggccgacc agacccgcaa
      421 gcgcgagtgc aatcaacatc gacaggaaaa tgcgtgtcca tcttttttgt ccgcgccacc
      481 aagcaatcgc gagcagcacg attccaagac cgaaatgttc cggccaatcg ccaaaacggc
      541 tcacattaag catgaaaatc ttcaccgctc ggttttgatg ttgcgcgagg aagtcacgga
      601 cggactcatc aaagtaaaac gcaactccaa tcgcgatcgc ggcgataatg gcgccgatca
      661 cccacagcaa aatcgaactg cgcttcacaa atacattccc gccacaataa ccgggcgcta
      721 ttttgagcgc gcacttgaaa tccgcaaccc gcgattacgc gcttctgttt ttcggttgtc
      781 tgctttttca cacgatcggg acgtggagtt tgcctttaat cgaccgtgat gaaccgcgct
      841 tcgccgaagc gtcacgcgag atgctcgagc gcggcgacta catcgttccg catttcaaca
      901 atcaattgcg tctcgacaaa cctcccctcg cctactgggc gcaggtcgca agctttgagg
      961 ttttcggcga aaatgacttt gccgcgcgtt ttccctcagc cgtcgcggcg gcgctcgtgg
     1021 cattgtcgat ctttgcgtgg ggaaatcgga tcgcgggcga gcggatcggt tggtgggcgg
     1081 cagttatttt cactctgtcg ctgcaaacat tcatccacgc gaaagcggcc gtggctgaca
     1141 tgtggctcgt gttgtttgtc accattgcgc actggagcgg ctacgaactg ctgcgagata
     1201 gtctaacgga tgacggcgga aaacgcccaa cgctcaactc cgataaatcg cgagcaggcg
     1261 cccaacgtcc aacatggaat tcagaacatc tacgatggtg gctcgcgttt tatgcggcac
     1321 tcggtctcgg gtttctggcc aaaggaccga tcggctggac tccgctgtta acggttgcat
     1381 cgataaaggt tttcgggcct gatctcgcgc tcgcaaagcg gttcaagttt ctgcgcggca
     1441 ctttgctcat gctagccatt gtcgcgcttt ggggcattcc ggctttgatc cgcacgcacg
     1501 gcgaatttct cgctatcgga atcgggcggc atgtgattgg gcgctcgttt gcggccatgg
     1561 aggggcacgg agccaactcg ttaggcattt atctcctcct gctaccgttt tatttcatca
     1621 cggtttttgt cagtttcttt ccctggtcga tcaaactgcc tcgcctcgcg cgccgactct
     1681 ggaacgaccg ggacaagatc gacaattatc tcctcgccgg tgtggctatc gtttttctta
     1741 tcttcagcct catctcgacg aaactgccgc attacatttt gccggcgttt ccgttacttt
     1801 cgcttctgct cgcgcggcga ttggttgacg agcagaagtt tttcaaggcc gcagcgatag
     1861 cagcagtttg catttatctc gtcgtggcgt ttgtcgtccc gccgctggct gcacgatgct
     1921 ttccggccaa gcagctctac aatgccgcgc gcgactcgct gcgccgagac atggaatttg
     1981 gtgccgtcgg cttcgaagaa ccgagcttgg tatggtattt tcgtacccga gtgaacggct
     2041 ggatgagctc actgcgcccg aaggatgcaa ccgaatttat gcagcgaccg ggggcgagat
     2101 tcatcgttct gccgaccaaa ctcgcgcacg aactgtttcc gattccgtcg cgagcctgga
     2161 aaatgttttc cgtatccgga ttcaacatcg cgaaaggcgc gcatgtcgag cttaccttgg
     2221 tgctgaagcc acaacagctg tagcggcgct ctgtgaccgt cgctcgaatt aaaattacaa
     2281 gtgcgacgct catagagcgc cgctacagag tcagtcgttg cacttcagtc ttcgtttgtt
     2341 aggagttgcc gacgaatgat tacctttctc gatggccggc tggtgagcgc gttaccaacg
     2401 caggcgattg tcgatgttgc cggcgtcggc tacgaagttc tgatcccgct ttccagctac
     2461 gatcgattgc cgcaagtcgg gcaaacaatt cacattctca cccatctggc ggtgcgcgaa
     2521 gatgcccatg tcctttacgg attcatgacc gtggccgagc gcgatttgtt tcgcctgctc
     2581 gtcaataatg tcagcggcat cggccccaag ctcgccctcg ccgtgttgag cggaatgagc
     2641 gtgacgaatt tcaaagccgc ggtggtgaac tccgatgttg ccgcgctcgc gaaaatcagc
     2701 gggctcggca aaaaaacagc cgaacgcatt attctcgagc tgaaggacaa aattggtgtt
     2761 gccgcggcgt gggaagcggc cagcgcggcc cacgcgccga cgccggagca gcaacaagcg
     2821 agtgaagctg tgctcgcatt gatcgcactc ggttacaaac agatcgaggc ggacaaagcc
     2881 gtgcgcgatt tgcagcagac tggcgaagcg aaatcggcgg aggagttgct caagctggca
     2941 ctgaagaaaa tagcgaccgg ccggtaaacg gagcgcgaac atccaaggtc tgcattgggg
     3001 attggacgtt ggacgttgga tgtcggacgt taggacaaac atgatcgccg tcggcgaaga
     3061 tcgactcgca acaatccgcg acgcttttcc gaaagccgga ctgttcgccg acaaagactg
     3121 gttggtctcg cccgatccgt ttccaattga gagcaaattt ctcgccgaac tcgagcagct
     3181 tggtcatcgg ctctacgttt ttcaacgcgc ctgcaaccag ctttaccagc tcagtatcaa
     3241 gggaaaacag ccggcgtggg tggcgcgata tctcgatgcg ggcaagccgc cggaactaat
     3301 tgagttctcg cgacgcaaac aaatccgcga cgatgtgccg cgcgttattc ggcccgatct
     3361 tatcctgaca gacgaaggct acatcatcgc cgagatcgat tccgtaccag ggggcatcgg
     3421 ccttacggcc tggttcaacg agacgtattc cgttttcgat ccgcacatca tcggtgggtc
     3481 cgacgaaatg ctcgctggct tcgaaaccgt gctgccgaat ggcggagaca tcgtcatctc
     3541 ggaggaatcc gccacatatc gacctgagat ggaatggctc gcggggcggc tgcaagatcg
     3601 gcaatccgcc tgtcgcgtcg tcgccgccgg gaattacgaa ccgcaagatg gccgcgccgt
     3661 ttaccgattt ttcgagctgt ttgatctgcc gaatattccg aagatcgaca atctgcttcg
     3721 cgcgaatgcc cagggccgcg tcacgattac gccgccagtc aaaccgtatc tcgaggagaa
     3781 aatgtggtgg gcgctttttt ggctgcggcc gctacgcgaa ttttggcggc gcgaactggg
     3841 cgagaaatat ttcattaaat tacaggaagc gatcccctat tcgtggctga tggacccgac
     3901 gccgttgccg caacacgccg tcattccgcg actagaaatt cacgagtggc gcgaggccgc
     3961 tggatttagt caaaagcatc gggagctgct tctgaaggtg agcgggtttt cgccgcttgg
     4021 ctggggcagc cgcggggtgt ggttcggtag cgacctgccg cacgccgaat gggaacgcaa
     4081 gatcgacaac gcgctcgcca ccttcgaatc gtcgcccacc atcatgcagc actttcacaa
     4141 gggccggctc ttttcgcacc gctattggga tgcggcgaca agtgaattaa agacaatgaa
     4201 cggccgtgtg cgtctgtgtc cgtattactt catcgacgcc ggtcgcgtga aactgtgcgg
     4261 cgcgctcgcg actatcgctc ctgccgacaa gaaattcatt cacggcatgc aggacgcgat
     4321 tctggtgccg tcggcacgac aataatttcg gagtgcaggc gattcggcag ctgccgcgcg
     4381 gctttttaaa cacgctcgag cacgtagctg cgccatcgtc gagcaaagga ctctgatacg
     4441 actagtaccc agcgctacac cgcacagtaa gttcgtgcgt atgaatcggc cccgcgggcg
     4501 taagctggct cgagtaaagg tgaaatgcgt ccacgcgaat gagaccgagg tcgagatccg
     4561 cattggtttg taaaaatttt cgcagcgtgt gggcggagac atcacgacaa cggccgagtg
     4621 taatgtgcgg atgccatggg cgaaggtcgg gttgcaagcc gacggcgagc gctgcgtcct
     4681 gaacgcgttt gtgaatttgg aacaaatgcg ggtggccttt cccgacgccg acccaaatta
     4741 ttttcggcgc gccttttggc ggaaacgtgc cgaggcaggt gattggcagg aaaaatgcgc
     4801 cgaattggat ggttctgagt ttttcgcgca gcgcaagatc gacatcttcg gggacgtgcc
     4861 cgaagaagcc gagcgtgaga tgcatttggt cgcggtcgct ccaacccacg ccgcgcatgc
     4921 gcggattaag atcgacaagt gcatcgcgcg tcgaatccgg gagcgagatc gcgacgaaga
     4981 gacgcttcga cataaggcca aaaacgtcga acgctcaacc cgcctgcgca atgctacggc
     5041 gcgggatcga tcacggctag gactattggc atgactcgtt atttttctcg atcgctcggt
     5101 cggccaaatg ggtcacccac tcgggggcaa gtcgcggaac atcgccgccg cgacgcgact
     5161 cattgcccgc gtcgcccgtt gttcccggaa gaaagcggtt cgcgagcttc attacgtacc
     5221 cggtgaggtt tggaaagagc gcattgccga cgatcgcgcc gcgcgccgcg aaggtgagcg
     5281 tgagcgaggg ctgcccgcgg cgagacgccg cgaggatttt tcgcgcggcg cgttccgcgt
     5341 tcatcgagat gagcggcgcg cccgcggaga cggagaacca agcgaactcg gcgtcatgtt
     5401 tgcctttgaa cttcgcattc acgtgcgagc cggtgcgcat gagcccgggc gcgacggtgg
     5461 tgacatagat gttctcgcgg gccaactcgg cacgaagtga atcggacaga ccggtcagtg
     5521 cgaatttgct cgcgcaatac gcggcgagat gcggcacggc gattttgccg ccgattgacg
     5581 agatgttaac tatgcgcccg ccgcgctggc gtcgcattgc ggggatgact tgcatgatca
     5641 gctcgagtgg cgcccagaaa tggattccca tgcagcgctc gaaatcttca cggctcatgt
     5701 gctcgaacgg gccgacttcg atgattccgg cgttgttgat caagatgtcg atcctgccga
     5761 agcgctctag cgtttgctgg actgcggatt cgatttgccc ccgatcgtgc aggtcgcatt
     5821 gaagcgtcag ggcttctccg ctacgcgcgg aaagatctga tcgagcccgc gcgagcgcat
     5881 cgggatcgcg cgcaagaata gcaacgcggc caccttcatc gcaaatctgc cgtgcgagca
     5941 cgagaccaag tccacgcgag ccgccactga tgagggcgac tttgtcttgg aacgaaaatc
     6001 ggctgcggta tacgcgcgac gcaatgcagc cgccgaagac gaggatgagg acggaaatga
     6061 accagcgcat ctggaaaatg attcgcgcgc aatctagcca atggattcga caaaggcagg
     6121 acgcctatgt cccgtgcctg cgattgaacc gggcgacttc gcgcgcggtc tcgcgctcga
     6181 cagcgcgctt tttcaaatcg gcgcgtttat ctcgtgcggt tttgccggcg gcgacgccga
     6241 gctcagcctt cagctttccg tttttccagt aaagcttcaa gacaacgaga gcgcggccgc
     6301 cgatttgcgt ttcgccgtac agtttgtcga tttcttgtcg atgtaaaaga agtttgcgca
     6361 cgcgtttggg cgcaggcacc tcatgactcg ccttttcgta cggctgaatg tcggcgttat
     6421 agagaaaagc ttcgccattc tcgacccgag cgaacgcgtc gttgatgttc gctttgccgg
     6481 cgcgaacgga tttgacttcg ctgcctttaa gcgcgatgcc ggcctcgtag cgctcgagga
     6541 tgtgaaagtc gcgcagcgcc ttgcgattga ggatcgactc ggcggccata gcagcacgcg
     6601 ccgcggcgtc acgagttacg cgttgtcggc ggaacccgaa agcgattcgt aactgagctt
     6661 gtcttcgatg atctccttca gggcgatgtc ggtgagcgac atacgcggcg tgatctctac
     6721 catcggccga tggccgaggc caagctgacg cacgcgtttc gagacaacat tgatgagcag
     6781 ctgctggttt gggatttttc gagcggcttc ctggaggagt tgagtcgtca tatggcgcaa
     6841 tgaatgaacg gcgcgcaatg tgccgttcgg gaattgaaga agggttggcg actcgcctgc
     6901 taaagcgacg agagtaagct gcggagcgag catgataaaa agaagcgttg actgtgcgag
     6961 cagcgcagcg gcttgtcaac cgatttgtgt gtgcgctgtg tcgatctcgc gggcgatgaa
     7021 atgctcctgc gatagcgaac cgattctgcc gtgtggccag gtggcctaac taccggatgt
     7081 cttccacgta gctggtaatg cggtagtcga cagcgtcccc ttcgagcccg agctcccgat
     7141 cgtaatgcca gctaacattg ccgttcgcat agaaggtctt gcatacaact gcgccggtga
     7201 gatcgggatt gccgttaata gagacatcag cgccggggcc gtagaagacg gccgcaaaat
     7261 tacccggcgg attaatatta atatgctgca tggtggaagg atcggtcgga cttatggcgt
     7321 agtactgcag attagcggca atgccgctct cgttgatgat gtcgcgtcct ttgacgtcca
     7381 tgttgccatc gacaaagatc ttcgccttga cgagtggctt ggtgtcgatt gagccgctaa
     7441 catcgcctgt gacatgcaat gcgacatagg ttttttgcga accaaacgaa ttgatagtta
     7501 actgcccaga caacgaagac agtaagtaga gggttgggtt cgcttccgta ccggcggctg
     7561 gaggggtgat gataacggtg ctcgtcaccg cggctggcga aggctgaggc aaaggcaggt
     7621 ttgtaggcaa ataatagcgc tcaacgctga aagggacgtt attatcgatc gtgccgctga
     7681 tgtattgcga ccgcacgatc gtgccgccgt tcgtcgcgac atccccgtaa atccagccac
     7741 gaaccgtagc cacggcggtg tcgatctcaa cattgccgct gcgcgaatcg ctgtaatgcg
     7801 gatcatttgg gctgagcgcg gcgaaataat atgcaccatt gcgcgaatcg aagctgtcga
     7861 tcatggcagc gtcgcccagg ccataaaacg tgccaatgca tttgatggct gcttcaaacg
     7921 gcgtaatcgg cgcgcaaact tgttcgattc ggcgcgtgat ttgaggctgc gcgacgggaa
     7981 taatggcctt gcttgtgcca tcaccgcttg gcccgtaggc ggcaatgaaa tggtcgtagt
     8041 tgaaatcgac cttacgcagc aggctatccc cgcgagagcc agtaagcatc cggtcgtcca
     8101 tcccggtacg tttgagcccg cgcactggag cggtgcctct gctgcggatg cgataccaag
     8161 ggttgccatt ccaggcgtcg taataaaaga tgtccaccgt gctgctcgtc gttaggttcg
     8221 agccgaacgt caccggtgtg ttggcgtaaa cgccaccggt gtaggtccag ccagcaaagg
     8281 cgtgggaagg atccatcacc gtgtttcgca cctgcgcata agcgatatcg ccgccgccct
     8341 cggcggcgtg taacgcttct ttccagccgc ggacctgatt agaagcggcg ttatagcggg
     8401 tggcgcagtt taacagcacg ctgccgccga tcatcgagac aatgagaatg gtgcacagcg
     8461 cgcagaccag gacgttggcg ttttcgccgg actgcggctg gttcatgtcg atcttgcgat
     8521 tgtcgatctc gccggcggca cgagatcagc cgcggcggcg attgcgcaag taagccgtgg
     8581 cgcaaaccgt cgtgccactg cgttcggccg tcgcattcgc ggatgtgaag ataggcaaga
     8641 acgtgaccgt ggtgctagtg tattcggtat ttgccagctc aacgtcggtc cactggggaa
     8701 cgagttgatc ggtggcagag gcgattgtgg ttacaacgcc attctcggtc cgcagaatcg
     8761 attggccgct gatcgcataa acgaccgagc tggtgctcga gccgtaattg acctgcgagc
     8821 cgctgcgcga gtaggaaacc gtcggcgtcc ggggcgtgcc aatgagcgat ggattagcaa
     8881 ccgcctccgg atcgccagct tgaatgatgt atttcggcac ggttatcgac acgctctgca
     8941 ggtcactcga cgtggtgacg atgagcccgc gcttcacgtc gcggctcaag tagtcaataa
     9001 tgcggatctg ctgcatgtgg gtcgcgaaaa agttatcgat cgcattgaag cttttctgta
     9061 gtccaattga cgccgtaatg gcggcgacga aaacgatcga gctgatgccg agagcgaccg
     9121 ttgtttcaac cagagtgaag ccgaggcgat tgcgcgtaga gtgtgcgatt ttcatttttt
     9181 ggtgccgttt gatatgatcg aggttaattg ttcgctgcgg gcacgggtgc ccaaagacat
     9241 catccacgaa atggcgacgt ctacttgaac gagcgtcgag ccgagatcag cggccacaga
     9301 gtcggcggtg acggtgccat tggcggagcg cgtgaactgc gtgaccccac tcgctgttgg
     9361 gtacgcgcta attttgacta cttcagtagc ctttttagcg aattcggaag cattggcagg
     9421 cgaactcaac aaattctgca cgtacgagct gttcgttaga tcgctaaagg cgaggttacg
     9481 cagagtttca gcccgatcat tcacaagctg gagcgcggcg attgattcct tgctggcgtc
     9541 gatgtagcga agacagacgg cgttcagttc gaatatcgat ccaaagaaca cagccaggag
     9601 gagcgctgcg actagtactt ccggcattgt tgttccggct tgggtgctca gtgagttccg
     9661 cttcatgcca cgtgattcta gaatgtataa aaattcagca gaattcatac ccatttcctc
     9721 tcaccgccga gaacgcctct cccgattgcg agttcatagc cacgctttcg caaaatcgac
     9781 attcagcggc ccttcttgga gctcgggccc gtgccaactg tcctcggttg gcgcaccgaa
     9841 ttcaggcttc gggacgagca cagcgcgcga tcaagagagt gcttgttctg cagcttttta
     9901 cgagcatgtt acggcgattc gccaaccaac ccgtgctcgt ggcagcggct ggggaatcga
     9961 atggcgggct gtggctcagc ttggtagagc gctgcgttcg ggacgcagag gccgtgggtt
    10021 caaatcccac cagcccgaca gttcatgtaa gcggcagcag cgctttgggc gaaagcgcgc
    10081 ggtgaggcat tgtgaatcag actgaattca gcgcgattgc tctttctggc tttacggtgg
    10141 cgttttttca cgtggcgatg ccgacgcact ggctgccgtt tgtgttaacc gcgcgcgcac
    10201 agcgatggtc ccgagcgaaa gccttggcaa ttaccgcgct cgctgccagc ggtcacgtcg
    10261 ttttcactgc tcttcttggg ttactcgtga ccgtttttgg attcgctttg catgagcgaa
    10321 cagcagcgtg gtttccacgc gtcggcggca gcgtgctggt gctatttggg ttgttacatc
    10381 tgtaccggca agtacttggc catgtgcatg gtcacagtcg cgtcattggg gaggatcctc
    10441 gcgttcacgc cgcgcatggc gcgttcggac atgagctgga gatcgagagg catgacgcta
    10501 cgcatgttcc agaccacgtc gccatcacga gcttactggc gctcttgacg ttctcgcctt
    10561 gcgaagcctt tgttcccttt tacatctcgg gcctgcacta tggctggttc gggttttggc
    10621 ttttaaccgc aattctgtca atcgctacct tgacaggcat ggtcgcacta acctggctca
    10681 ctctcgttgg gataagacag atcgcactgc gtcagctcga acgttacgag cttggggtga
    10741 tgggcatttt gctttgtgca gtcggtgtgc tgatcattat cttcaaccca tgagaagggt
    10801 cgatgcccga acagctaatt acgatcggtc aactcgggcc tcggaaaaga gagttgcagg
    10861 aacttgtctg aactgatggg attgcgaccg gctgatcgga agcgccactt gcacgtatgg
    10921 ggtcgttaaa aaagagacgc aaagcgaaaa tatccaaaca caagcgccgt aaacggatga
    10981 agctgaaccg gcataaaaag cgcctccgct acaaagcgta ggcgatctgc gtttgaacct
    11041 tacaccggcg gcgttggttt gcagaagcta acccgcgcgg ctagactcgc tcatgcgtgc
    11101 gcggaacagt tacatgggtg cagttgttct gttgagtttg tgtttcgcgc tgccctgctt
    11161 cagcggcaat ttcgtacgtc cgcgtgaagc cctgccaacc ctgtcgccgc cgaagctacg
    11221 aaccggtgcg atccctcagg atgactcatt cagtgccgtc gccgccaagg acctcactct
    11281 gcgagtcgac ggcgaacata aagccgaagc gctcgcgcat ttcgtcgaag gcaatgcgtt
    11341 cgaagaaaac ggcgaaatgg ataaagcgtt gcacgcgtac cgcgaggtac taaatgtcga
    11401 tcctgggcaa agcgagcttg cctcccacgt ggccgagttg ctcagccggc aaagcgattt
    11461 cccgcaagcg atcgatgtcc tgaaagacgc catcgctgtc aatcccaata aggcggagcc
    11521 gtatctgcag ctcgcgttca tttatgcgag gtacctcaaa cgatccgatc aggccatcga
    11581 atatgtgaat cgcgcgatcg cacttgatcc acgcgacatc gacgcttatc agcgcctgtt
    11641 cgaactactg tcggccgctg gcgacgataa aaaagcgctg caagcgctgg agcgcgccgc
    11701 gaaggtgcag actgacaacg ccagcttttg ggcacggctg ggaaaactct acgctgctgc
    11761 cattttcgag ccgggtcgca accctaaacc caccgagagg gcgcgggtaa acgagatttt
    11821 caagaaagcc gccgagaacg gcaaggatga cgcgaacatc ctcaaggatg tcgcggattt
    11881 ctacgcttct tcgcagcaat tgcagcaagc gatcccgctt tacctcaggg tcctcgagtt
    11941 acaaccggat gatgtcaacg cgcgtgaaaa actcgcgacc ggctttgtgc tcaccaacca
    12001 gcgagcaaaa gcaatcgaaa tgcttgcgca gcttattaaa gagcaccctg agaagtacga
    12061 gccatatgat ctgcttgccc aaattttcga ggacgacgct cgctcgctcg agcgcgcgaa
    12121 tcgcaaccaa gaagcaaagc tgcaatatga acaggcggca gcaaactacg agcagagcct
    12181 cttaatcgct ccgacccatc cgaacacgta cttgcgcctc gcggagctgc tgcttggacc
    12241 gtgcaaagat gcacaacgcg cagtgaaact tctgagcgaa gcgcggcggc gtttcccgaa
    12301 tgcgccagag ctcatctatt atatggctct cgcgcaacgc gaagccaagc atccgcaaga
    12361 agccgttgcc acattcgaag aggcacgcca tgaagccgag ctggaggaca gcgagatggc
    12421 caatgcccgc ttctatttta actacggagc gactgccgaa caggccggct tatatgagaa
    12481 agcagccgac ctgtttcgca aatctatcgc catggacccg gcaaacgcgg ccgacgcctg
    12541 caactactta ggctacatgt gggtgcagca gaacgcgcat ttggatgaag ctgaagcgat
    12601 gatcaaacgc gcgctccaaa gcgaaccgga taatggcgcc tatctcgaca gcctgggctg
    12661 gcttgagttt cgccagggca aattcgagca ggcattgaag gatttgcttc gggcagccaa
    12721 gagtctgacg cgggatgatc ccgtcgttta cgagcacata ggcgatgctt acttgcagct
    12781 gaagcgaatg cctcaggcgc tcgaggcttg gcagaaggca cttgcgctcg acccagaaaa
    12841 caaaacgctc gccggaaaga tcgagagtac caaaacgatg atgagcaaaa gcgaaccgcc
    12901 aaatagcggg gcgtgacaac tcaacggaga tgtcgatctt gctccgccct cgcctcgctc
    12961 gagcagacac acgcgcgcca cgcggatgag aaactacccc actgcttggc agcgcaagtt
    13021 gatgtgggcg gcgctttccg ccttttttac cgtgttgctc atagcggtag ccgcttcagc
    13081 gatctgggtg gtcgcaaatc tgcttagctt tcttcagccg attctcatcc ctgtcgcgat
    13141 cgctgtcatt ctcgcgtatc ttttcgatcc actggtcacc aagcttgaac gcgggctgct
    13201 tacccgcacc aaagccatcc tgctcatttt cgcggtcgcg tttctcttcc tcgccggctt
    13261 gatagcttgg gttgcaccga cggtttcgat gcagagcgcg aatttcgcgc gcgagcttcc
    13321 taactacacc cagaaggcgc gcgaccgtat tgttgacgtt attgttcgct tcaatcgcac
    13381 tttcgccgct ccgagccggc gcggcaaaac cgtatcgccg agctccggca tcgttaactt
    13441 gcttttagga actccatctc cgcaagcctc tgccgccgcc tcagccaccc ctgtgcctcc
    13501 gccggccgct gttgcaccgg gtgcaagcgt tacaccggcg ccggtcaaac tcacctcggc
    13561 agatcggcag cgcttgcaag cctatttcga tcagttgatt ccgaatctgg agcgaaacat
    13621 cccgacactc gcggcacagc tctgggacat tttgaagaag agtattggcg ggtttctcgg
    13681 gttcaccgga tttctgctca gtctggtgct ggtgccgatc tacttgttct tcctgctcaa
    13741 agagcgaccg cgaatcgaac gccgctggaa ggattatctc ccgctgcgcg attcgccctt
    13801 gaagaatgaa atcgcggaag ttctatcgca gatcaacgac tacatcatcg cttattttcg
    13861 tggtcagctt ctcgtctgtt tggtcgacgg cgttctcatc ggcagtgctc tgatgctttt
    13921 cggacttaac ttcgcggcgc tcatcggcat gctcgttgtc atcctcacga tggttcctta
    13981 catcggcatc gtcctgtgct gggtgccagc ggtactgatc gcggcatttc aatggggcga
    14041 ctggtggcac ccgttctggg tcaccgtcat ctttgttgtc attcaaaacc ttgaaggaat
    14101 gttttacgcg ccgcggatcg tcgggaattc ggtcggcctg catccgatga cagtgattgt
    14161 gtcgatcttc gtctgggggc ttctgattgg cggagtgctc ggaccaattc ttgccgtccc
    14221 attgacagcg acgctcaagg tgctgctaat gcgttatgtt tgggcacccc gcgtccgcga
    14281 agaagagatt gcggaaatcg agcaggtccc ggtcgtcgca gaatcggaac gcgcgaaagc
    14341 ttagccctgc tcgaccctcc cgcggtgtgc gatgcagttg cgttctccct cggccggaac
    14401 atacaactaa ataaatggcg gaaaaatcaa gacggcggcg catcagaaga tctcgccggt
    14461 cctctggccg caagtttgtg ccgcgcttca tccgtcactc gccccgggtg cggctgtttg
    14521 ctgtcatcgg gattgctgct gccattttcg tgatcgcgat gtttttcctt ggttcggtgg
    14581 ctaaggctta caatcattgg cgacagaatc ggcttttgca gcgtgcgaac actctgttga
    14641 acgaggcggc ctcgaaggag atagccactg caacacgaaa gaacgacctc gaacaagcaa
    14701 tgcggatcgg caaagaagtc ttgcgacttc gtcccgattc gctctctgca tactacgtgc
    14761 tggccgaggc tgccgaaaaa agcaatcttg aggaaacagt ggggtggcga gcgcagatcg
    14821 cgcgtcttct cccgcacgat ttggacagcc agcttaatct cgcatcagcg gcattgcggt
    14881 ttggccagct cgacctcgca cgcaaggcgc tcgaaaaagt tccagccgtc gaccgcgacc
    14941 gtgctccatt ccacgttgtg gccggttggc tcgcccgcgc ggagggcaac gtctccgagc
    15001 aagaacacca atttgctgca gcggtgaaga aggagccggg caatgatctt taccaattca
    15061 atttggccgt cttgcgcatc cgctcgcctg atccagacaa gcgcgccgat gcgcgtgcca
    15121 ccctcgagcg gttgagtaaa acgaccggct tccgggccgg tgcactgcgc gccttgctca
    15181 acgatgcggt cgagcgcagc gatttcgccg cggccgacag catcgcccag cagctgcaaa
    15241 tgatccagca ggtcaccttc aatgactatc tgctttgcct gaacttctat cgcaagcttg
    15301 acgacaaaaa atttggcgcg ctgctgcaga aagtgaaagc gtttgccgcg cacgatccct
    15361 ccgaggtcgc gctgctaatg agctggatga acaccaacgg tctcgccgct gaagtgctgg
    15421 agtggctcga taaattgccc tcggaaattt ccactcaccc gccagtcgcg atcactgtcg
    15481 cggaggcatt cgcggccgca aaaaactggt cgcgcctcaa acgttggacc cgcaccggct
    15541 cctggaaaga cgccgattat ttacggcttg cttatcaggc gctggccgcg cgccaatcgc
    15601 ggcaatccgg cggcgaagcc gagttcaatt cgctctggcg ttcagccgaa cgcgaggctg
    15661 acgaacaacc cgagtaccag gcgcaactcg cacgcttggc cacgaaatgg aatctcacca
    15721 tcgaggccga tgcattatgg tcgcgagtcg cggtaacgcc gccgctgcgc cgcgaggcgc
    15781 tcaacgccct ctaccatatc tatcgcgaga acaatgagct ggcaaaaatc tacgacgtgc
    15841 tgcagcgcct ccaccaaact tcgcctaacg aaccggcgat cacggccaat ctcgcgcggc
    15901 tgggcatgaa cctcgaccaa aacaccgacc aggcccatcg cctcgccaga gaagcttatg
    15961 agcgcgcacc ggaggatacg aattgcgccg tgacctacgc ctattcactc tacggcatgg
    16021 gccggaccag cgacgcgatc gaggttttga aaaaattgcc gccggaacaa ctgcacgatc
    16081 cacatgccgc ggtttacgtc gccgcgatgt tgctcgacga aaaccagccg gatgcggccg
    16141 gagagtacat ccaggctgcc gcgcatagcc gcctttatgt ggaagaaaaa tcgctcttgg
    16201 aggaggcgaa gacgaaacaa gcagcgctgc ttgcggcggc ttcgctttcc ccctcgccag
    16261 ccgattcacc cgcggttctc ggttcctcgc cggttgcgcc aacggctacg cccgtgccga
    16321 gcccgacagc ttcgcctctg ccttcaccat cgccgcgctg acttcgggat cattcgtcga
    16381 ttgcctttgt ccagtcgcta atttccgccg gtcccatttg cacccgcgat tccaagcgcg
    16441 catcacgaaa accgcgctct ttcacgtcga tcaaatattg agcacgggac agcaacgtgc
    16501 ctcgacaaat tcgctcggcg agcggcttcg cttcatttgg gaaacgttgc accagctctt
    16561 ccattacgtg cttgggaatt cgcccccgct cgttcggata aatgtaaccg aacaatacga
    16621 gatgactcag cagcacttcc caatctggtc cgaaccggtc aagcaaatgc cgccaatcga
    16681 tccgctcggc gcagctctga aatagatgcg ccacatcggc gccgtcgtaa cgctcgcgtt
    16741 ccatgatgaa cgctttcatc cacacaattt cctcgggtgg gcatagctga actgcgctcc
    16801 ggagtagctc ggcacgctct gcccgcgcga accacaaatc gtccacgccg cacaatccgt
    16861 ttccggcgcg aaaaatcaga tcgatgagat cggtgccggc tcgcaccttc gccagccaat
    16921 gcgggtaagt tttttccgct ccatagccgg cgcgctgaaa cgcgtccaac gcacgatcga
    16981 catctctcgg ccgcagaaat aaatcgaagt ctttcgtgtc acgggagatt ccggtgtaga
    17041 acgcaaacgc gtacgcgccg ccaaccagaa acggtactcc tgcgcgctcc aatacgcaca
    17101 tcgattcgcg gtagaaatcg gcggagcctt tttccccatt gggcatcacc ctcaatacga
    17161 tgccgcttcc tcatatgcgt gcataaccat tgtcgatcct cctccaggtg tcgcaaccgc
    17221 tcggccgatc caaaccgaac gtccgctacc tgacgcgcgt ccgtattact ctttcgaacc
    17281 gatggctcag cgcaaactac gcatcgccgc taccgccgat attcattacg gcaaacattc
    17341 acggggcgcg ctgcacgaca cattcgagga gatctcgcgc gcggccgacg ttctcctgct
    17401 ctgcggcgat ctgaccgatt acggtctcgc cgaagaagcc gaacaactcg tcgccgacct
    17461 ccgtgccgct gttcgcatcc cgacaatcgc cgtgctcggc aatcacgatt tcgagtccgg
    17521 cgaatcggag cttgtgtgca aagtgctcga tggcgccggt gtcaacgtgc tcaatggcga
    17581 ggccgtcgaa atcgcaggcg ttggttttgc cggcgtctgc ggattcgccg gcgggttcgg
    17641 ccgccggatg ctgaatgcgt ggggggaacc gctcatcaaa caattcgttc aggaagcact
    17701 cgatcatgcc gttcgactcg agcaggcgct cgcgaaactc cgcaccgata ggcgcattgc
    17761 cgttctgcat ta
//
LOCUS       DASTDG010000029         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_651360, whole genome shotgun sequence.
ACCESSION   DASTDG010000029 DASTDG010000000
VERSION     DASTDG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651360"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..613
                     /locus_tag="VFO30_01060"
     CDS             212..613
                     /locus_tag="VFO30_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9417904.1"
                     /translation="MFTKLTAKRAGFTLVEIMIVVAIIALLAAIAVPGFLRARKRSQA
                     SRILNDLRLIDSAVDQYAIEHGKKTDDTVLVADWTAYVKKNAVLANTGQDVLGNSYGD
                     QTVDQVPQVPSATYNSLSDVAGTGFWSPYGP"
     gene            681..2858
                     /locus_tag="VFO30_01065"
     CDS             681..2858
                     /locus_tag="VFO30_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417905.1"
                     /translation="MSNLQAAGSRRFGRSRIAAKLTRATGSLACNIAGYTRKTSVRVL
                     LLLLVGAAAHAPALQGEFVWDDQYLAHDNPLIKSPILVLETFRQYLFLDSFSTHYRPV
                     QNISFMLDYFFWNTNTFGFHLTNVLLHVTGGVLLYFLLRQLFASLIFPRASVAVRTRS
                     ATRVQWTSLSAFLVSMVWIVHPVHSAAVDYISGRADSLAFVFACAGWLLFMRGRNGAH
                     RGIQIGCYSLAGLLALLALLSREVAVVWLLLFLVHLFFVERHKAIGVGQRVVALCSCL
                     LLIVIYLGLRELPSPRVSASVQDNWSAPVRAALMARALGDYARLLVFPTNLHMERTVF
                     NTAGIGNNSEWRQAIGAEYLSIIGLLFFAVLICGSLKRGRAQTMRIFGAAWFLAGYLP
                     ISNLIQLNATVAEHWLYLPSVGFLIFIAGSLLELPRAWQNRVAVCALSTVMALGTASF
                     VRSSDWVSGETFYKRTLASGGTSARVITNLARIYAERHDYPAAERMLRRVMEVAPDYS
                     IAINNLADLLFHNGKMAEAEKLFRHSAEVAAERSRYPRPWIGAVNVAIVRHKARDDEQ
                     ALATLAKERAAHPNVWDIVSYESEILRQSKGPEAALPLVKEFVRANWWHYGAALALGR
                     LYAEKGDFDRAHNALRHASRLDVHDTQALSLLATMSMQQNHLEDACRIQRRAIARQPD
                     EPRQYILLSNILEKMGREDEARAALAHVSQLREFAHHESTLLN"
     gene            2936..>3901
                     /gene="ilvC"
                     /locus_tag="VFO30_01070"
     CDS             2936..>3901
                     /gene="ilvC"
                     /locus_tag="VFO30_01070"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9417906.1"
                     /translation="MAARIYSDNDVDLAWLKGKVCAVIGFGAQGHAQALNLRDSGFSP
                     VIGLYRGSPSRRVAKAHGMVVVDTPEAVRRGDIIFLALPDTSMPKIFENEIAAALQPG
                     QTLLFAHGFAIYYRTIVPRRDVDVVLVAPKGLGPMVRREFLAGRGVPALVAVHQNPSR
                     RAKKTALAWAKAIGCTRAGVIETTFREETETDLFGEQAVLCGGTSALIRAGFETLVDA
                     GYPPELAYFECLHELKFIVDLIHEAGIAGMTKLISDTAKWGEFVVGPKIIDRAVKKRM
                     AAALREIRSGKFAREFIKQMSSGRKRYEQLLRTAAHHPLERVGRKL"
BASE COUNT          767 a         1108 c         1120 g          906 t
ORIGIN      
        1 gccgtggaca gcatcccgca ggtgcctacc accgactaca acgctttgtc agatgtggcc
       61 ggcaccggct tctggtcccc ctacggtccg tagcagaatc tgacgattca tccacctgtc
      121 attctcgcca acgagagtga cagggaatga gtcgtagggt gcgaatagtg ccctcacgac
      181 tggagtaaaa gatagaaaga aatacgcact tatgtttacc aaactgacag ccaaacgtgc
      241 gggattcacg ctggtggaaa tcatgattgt ggtcgcgatc atcgcgctgc tcgcggccat
      301 cgccgttcct gggtttctcc gggctcgcaa acgctcccaa gccagtcgca ttctcaacga
      361 tcttcgttta atcgattctg ctgttgatca gtacgccatc gagcacggca aaaagactga
      421 tgacacagtt ctcgtcgccg actggacggc atacgtgaag aaaaacgcgg ttctcgccaa
      481 caccggccag gatgttctcg ggaattctta cggcgaccaa accgtcgacc aggtaccaca
      541 ggttccgagc gcgacttaca attcactttc ggatgtcgcc ggcaccggtt tctggtcgcc
      601 gtacggcccc taaatagctg ccgcttccaa tgagagcgtc cattcccaga tgggaaggac
      661 gctcttgtgc atttcaggca ttgagcaatt tgcaggcggc cggatcgcgc cgcttcggta
      721 gatcgagaat cgccgcgaag ttaactcgag cgacaggctc gctcgcctgt aacatcgcgg
      781 gctacacacg caaaacctcg gttcgcgttt tactgcttct gcttgtcggt gctgctgcgc
      841 acgcacctgc gttacaggga gaattcgttt gggacgatca gtatctggcc cacgacaatc
      901 ctctgatcaa gagcccaatt ctcgtgctcg agacattccg ccaatacctc ttcctcgact
      961 cgttttccac gcactatcgg ccggtgcaga acatctcttt catgctcgat tacttttttt
     1021 ggaacacgaa cacgtttggc tttcacctga ctaacgtgtt gctccatgtt acgggcggcg
     1081 ttctgcttta ctttttgctg cggcagttgt tcgcgtcgct gatctttccg cgtgcttcag
     1141 ttgctgtgcg gacccgctcc gcaacgcgtg ttcagtggac ttccctcagc gcattcctcg
     1201 tgagcatggt ttggatcgtc catccggttc acagcgccgc agtcgattac atctcgggac
     1261 gcgcggacag tctcgctttt gttttcgcct gcgccggatg gctgctgttc atgcgcggcc
     1321 gcaacggtgc gcatcgaggc atccagatcg gctgctattc cctggccggc ctgctggccc
     1381 tgctcgcgct gctatcgcgc gaggttgcgg tggtgtggct attgcttttt ctggtccacc
     1441 tcttttttgt cgagcgacac aaagccattg gagtcggtca gcgcgtcgtc gcgttgtgca
     1501 gttgtctgct tctgatcgtt atctatctgg gtttacgaga acttcccagc ccgcgtgtaa
     1561 gcgccagcgt gcaggacaac tggagtgcgc cggtgcgtgc ggcattgatg gctcgtgcgc
     1621 tcggcgatta tgcccgattg ctcgtctttc cgacaaacct gcacatggag cgcacggtgt
     1681 ttaacactgc cggtatcggc aataactccg agtggcgaca agcgattgga gcggaatacc
     1741 tgtccattat cggcttgctc tttttcgccg tgctcatctg cggcagcctt aagcgcggcc
     1801 gtgcgcagac gatgcgaatt ttcggcgccg cttggtttct cgcgggctac ttgccgatct
     1861 cgaatctaat tcaattaaat gcgacggttg ccgaacactg gctgtacctg ccgagcgtag
     1921 gttttcttat ttttatcgcc ggttcgttgc tcgagttacc acgcgcgtgg caaaacagag
     1981 tcgccgtatg cgcgctcagt accgtcatgg cactcggtac ggctagtttt gtccgcagta
     2041 gcgattgggt aagcggcgag acattttaca agcggacgtt ggcgagcggc ggcaccagcg
     2101 cgagagttat cacgaatctc gcccgtattt acgccgagcg acatgactat cctgccgcag
     2161 agagaatgct gcggcgagtc atggaagttg caccagatta ctcgatcgcg atcaacaatc
     2221 tcgccgacct cctgtttcac aatgggaaaa tggctgaagc agaaaagttg tttcgtcact
     2281 cggccgaagt cgctgccgag cggagtcgtt atccgcgacc gtggatcggc gcggtcaatg
     2341 ttgccatcgt tcgccacaag gccagggacg atgagcaagc cctcgccact ctggcgaaag
     2401 aacgcgccgc gcatccgaat gtttgggata tagtcagcta cgagtctgag atcttgcgtc
     2461 aaagtaaggg accagaggca gccttgccat tggtcaaaga gtttgttcgc gcgaattggt
     2521 ggcattatgg cgcggcgctc gctttgggtc gcctgtatgc cgaaaaaggc gatttcgatc
     2581 gtgctcacaa tgcgctgcga catgcgagcc ggctcgacgt gcacgatacg caggcgctta
     2641 gcctcctcgc cactatgagt atgcagcaga atcacctcga agatgcctgt cgaattcagc
     2701 gccgcgcgat cgcgcgccaa ccagatgagc cgcggcagta catcctgctc tcgaacatcc
     2761 tcgaaaaaat gggccgggag gatgaagcac gcgccgcgct tgcgcatgtg tcacagcttc
     2821 gcgagttcgc acatcacgag tcgaccctgc tgaactaact gcccgctcct gctcgcatgc
     2881 ctttgacctg gtcgcggcac gccgctacga ttgcgggccc gtcttcggct cacttatggc
     2941 cgcgcgcatt tattccgata acgatgtcga tctcgcgtgg ctgaaaggaa aagtctgtgc
     3001 ggtgattggc tttggtgcgc agggacatgc ccaggcgtta aatctcaggg acagtggctt
     3061 ctctccggta atcggtttgt accgcggcag cccctcgcga cgtgtggcga aggctcacgg
     3121 catggtcgtt gtggacacgc cggaagcggt ccggcgcggg gacatcattt tcctggcgct
     3181 gccggacaca agcatgccga aaattttcga aaatgagatc gcggcagcgc ttcagccggg
     3241 acagacgctt ctttttgcgc acggctttgc catttattat cggacaattg ttccgcggcg
     3301 agatgtcgat gttgtgttgg ttgcgccgaa gggtcttggc ccgatggtgc ggcgcgaatt
     3361 tctcgctggg cgcggtgtgc cagccttagt agcggtacat caaaatccga gcaggcgcgc
     3421 caagaaaacc gcgttggcct gggccaaagc gatcggctgc acccgcgccg gcgttatcga
     3481 gacgacattt cgcgaggaga cggagacgga cctgtttggc gaacaagccg tgctttgtgg
     3541 cggtacgagc gccctcatcc gcgctggttt tgaaacgctc gtcgatgccg gatatccgcc
     3601 ggagctcgcg tatttcgaat gtctgcatga gctgaagttt attgttgatc tcattcacga
     3661 agccggaatt gccggcatga ccaagctgat ctcagatacc gcgaaatggg gcgaatttgt
     3721 cgttggaccg aaaatcattg accgcgcggt gaagaagcgc atggcagcgg ccttacgcga
     3781 aatccgctcc gggaaattcg cgcgcgaatt cattaagcaa atgtcgagtg gtcgcaaacg
     3841 ttacgagcaa ttgcttcgca ccgcggcgca tcacccgctt gagagagtgg gacggaaact
     3901 c
//
LOCUS       DASTDG010000030         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_834823, whole genome shotgun sequence.
ACCESSION   DASTDG010000030 DASTDG010000000
VERSION     DASTDG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834823"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFO30_01075"
     CDS             <1..1065
                     /locus_tag="VFO30_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417907.1"
                     /translation="LAREAESSSGVLARAFRRAARLAFLWPDEVADLVAQNRPLRELA
                     GVGPFIEKQIRDWIDKPPALQGKVPPLRRDFFAMADARKLLSAHPAWAKLLRGDLQMH
                     TQWSDGSGSIREMAQAGESRSYEYIAITDHSKGLKIARGIDEAALRKQGDEIAGVNMS
                     ILRSGGKLAVLRSTELNLKPDGEGDMQPGAMLRLDLVLGSFHSVLRVKHDQTARYLAA
                     LRNPQIQILGHPRGRIYNYRLGLAADWPRVFAKAAQLDKAVEIDCYPDRQDLNLSLLK
                     IARREGARISLGTDAHHPWQLDFIDLGLAAALTAKIPAERIVNFMSILQLKQWVASVR
                     ARTAQGHLSPRRSVRRGGRG"
     gene            complement(1062..1577)
                     /locus_tag="VFO30_01080"
     CDS             complement(1062..1577)
                     /locus_tag="VFO30_01080"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9417908.1"
                     /translation="MAGKINFLGHPVHPMVIVFPLGLLPTAVACDIIYLVRNNPNWAH
                     ISYWLVAAGVLSGVVAAFFGIADWVALPAGTRAKRLGGWHARVNITMVVLFAISWWLR
                     RAAPDHPSSLAIGLGVIALLFGLIGGWIGGELVYRLSVGVDFDAHVNSPSSLSSRPAS
                     DNKETLPASQR"
     gene            complement(1644..>2238)
                     /gene="msrA"
                     /locus_tag="VFO30_01085"
     CDS             complement(1644..>2238)
                     /gene="msrA"
                     /locus_tag="VFO30_01085"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9417909.1"
                     /translation="ISVWIALLIVDLTNGQTPTFTRTKTAVLAGGCFWCIQPAFDKAS
                     GVIKTVVGYCGGTEPNPTYELVTSEKTQYRESIEITYDPAKISYARLLDIYWRQIDPT
                     QSDGQFTDIGPSYRAAIFYATAQEKKDAEESKEKLGRSGKFKKPIVTEILPLGKFYPA
                     EPYHQKYYEQNPEHFEAFENGSGRTAFQKKNWGGAQP"
BASE COUNT          474 a          666 c          638 g          460 t
ORIGIN      
        1 cttgcacgcg aagcagagag tagctccggc gttctcgcgc gcgcatttcg ccgggccgcg
       61 cggctggcgt ttctctggcc ggatgaagtt gccgatctag tcgcgcaaaa tcgtccgctg
      121 agagaactgg ctggggtcgg gccctttatt gaaaagcaaa tccgcgactg gatcgacaaa
      181 ccgccggcgc tccaaggcaa agttccacca cttcgccgcg atttcttcgc gatggcggat
      241 gcgcgcaaac tgctgagcgc gcatccggcg tgggcaaaac tcctgcgtgg cgatctgcaa
      301 atgcatacac agtggagtga cggctccggt tcgattcgcg aaatggcgca agccggcgag
      361 agccgcagct acgagtacat cgcgattacc gatcattcga agggactgaa gatcgcccgc
      421 ggaatagacg aagcggcctt gcgcaaacaa ggcgacgaaa ttgccggtgt aaacatgtcg
      481 atcttgcgca gcggcgggaa acttgccgtg ctccgctcaa ctgaactgaa tctgaaacca
      541 gacggtgaag gcgacatgca acctggggcc atgctccgcc tcgatcttgt gctgggctcg
      601 tttcattcag tgcttcgcgt aaagcacgat caaacggcgc gatatctggc ggcgctgcgc
      661 aatccgcaga tccaaattct gggccacccg cgcggacgca tttacaatta ccggctcggc
      721 ttagcagcag attggccgcg cgtgttcgcc aaagctgccc agctcgacaa agcggtcgag
      781 atcgattgtt atccggaccg gcaggacctc aacttgtcgc tcctgaaaat tgcgcgccgt
      841 gaaggtgcgc gcatttcgtt aggcacagac gcccatcatc catggcagct cgactttatc
      901 gacctcggct tggctgcagc attgaccgca aaaatcccgg ctgagcgaat cgtaaatttc
      961 atgtcgattt tgcaactgaa acagtgggtc gcgagcgtgc gcgcacgcac ggcgcaaggt
     1021 catctgtcgc cgcgtcgctc ggtcaggcgc ggcggtcgag gttaacgctg gcttgccggg
     1081 agcgtctctt tgttgtcgct cgctggacgg ctcgacaacg agctaggcga attcacatgc
     1141 gcgtcaaaat ccacccccac actgagccgg taaaccagtt caccgccgat ccagccgcca
     1201 atcagtccaa agagcagcgc gatcacgccg agtccgatcg cgagcgagct gggatgatcc
     1261 ggcgcggccc ggcgcaacca ccaactgatc gcgaacagaa cgaccatcgt gatgttcacc
     1321 cgcgcgtgcc atccaccaag ccgtttcgcg cgcgtgccgg cgggaagcgc cacccaatca
     1381 gcgatcccga agaatgcggc aaccactccg ctgagcacgc ccgccgctac cagccaatag
     1441 ctaatgtgcg cccagttcgg attgttacgg accaggtaaa tgatgtcgca tgctacagcc
     1501 gtgggcagca gcccgagcgg aaaaacgatc accatcggat gcaccgggtg accgagaaaa
     1561 ttaattttgc ctgccatagc caaactcctc tccttcagaa tttcgcagcg gctgccatga
     1621 tcggactgct gcctgcgaga aaactacggc tgcgcgccac cccagttctt cttttgaaac
     1681 gctgttcgcc cggagccgtt ctcgaaggct tcgaaatgtt ccggattctg ctcgtaatac
     1741 ttctgatgat aaggctcggc gggataaaat tttccaagcg gtaggatctc agtcacgatc
     1801 ggttttttga acttgcccga gcggccgagc ttctctttcg attcttcggc gtcttttttc
     1861 tcctgcgcgg tggcgtaaaa aatggccgcg cgataactgg gaccgatgtc ggtgaactgt
     1921 ccatcgcttt gcgtcggatc gatctgccgc cagtaaatgt cgagcaaccg tgcgtaggaa
     1981 atcttcgccg gatcgtaggt gatctcgatc gactcgcggt actgcgtctt ttccgatgtg
     2041 actaactcgt aggtcgggtt cggctcggtc ccgccgcagt agccgaccac cgttttgatt
     2101 acgcccgacg ctttatcaaa cgcgggttga atgcaccaaa agcagcctcc ggcgagcacg
     2161 gcggtttttg tccgtgtgaa cgtgggagtt tgtccatttg taagatcgac aatcagcagc
     2221 gcgatccata ccgagatt
//
LOCUS       DASTDG010000031         5591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_382041, whole genome shotgun sequence.
ACCESSION   DASTDG010000031 DASTDG010000000
VERSION     DASTDG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382041"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFO30_01090"
     CDS             <1..537
                     /locus_tag="VFO30_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417910.1"
                     /translation="TFGAVFFFIYCQLRWGHWDIYMLTQSSGWAIVPDYLAVLKPSSY
                     RWLVPGLNDPTAMSQMSMTFGALFFLLIAIVEIVPAIRRNTNWAARIGIYFCAAVIYY
                     ISVSGVASVDMESMLRYEFSVHALIVLALLHFLRQFAKPPVYVRAFGMAAVALAAVAG
                     LMLQGWYVWNFTRGNWVA"
     gene            539..2059
                     /locus_tag="VFO30_01095"
     CDS             539..2059
                     /locus_tag="VFO30_01095"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9417911.1"
                     /translation="MKTTVAIIGAGPAGLTAAYLLCKNDVDVVVLEADPVYVGGISRT
                     ATYKGFHFDIGGHRFFSKSKAVEDFWTEILPNDMLHRPRSSRIFYGGKFFTYPLKPFE
                     ALIKLGVFQSILCVLSWLKARLLPVRDPRNFQDWVSNQFGRRLFKIFFKSYTEKVWGM
                     SCKEISADWAAQRIKGLSLGSAIKNALIPQRYNGDRSHVIKTLINSFRYPRKGPGMMW
                     EACAEKVQAMDGQIHMGCKVTGCAYDDVTSTWTLRFQDRQANVQTIEADHVISSAPMR
                     ALVRGLTPRVSERTLRAADRLKYRDFLTVMLILKERNKFADNWIYIHDPSVKVGRVQN
                     FRSWSPEMVPDPDKACYGLEYFCFEGDGLWESRDEDLIELGKRELIKIGLAQEGDVVD
                     GTVVRQKKAYPVYDDDYAMHVATIRQELEQRYPNLHLVGRNGMHKYNNQDHAMMTAML
                     CVENILANTRLYDLWQVNQDAEYHEAGAAPAEESNGAALRLVPTRVVATPELAPEG"
     gene            complement(2234..3019)
                     /locus_tag="VFO30_01100"
     CDS             complement(2234..3019)
                     /locus_tag="VFO30_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET9417912.1"
                     /translation="MPLLEGLLRLTRWALPLIILAAIILPQAIRILREYERGVIFRLG
                     KLLGAKGPGLILLIPIVDRMVRMDLRVVTISVERQEVMTRDNVPVTVDAVVYFRVVDP
                     QAAVVKVENFLKATSLMSQTTLRSVLGQAPLDDLLSQRESINLKLQEIIDRQTEPWGI
                     KVTAVEVKDVALPDTMKRAMAKQAEAERERRAKIVNAEGEFQAAEKMVQAAAMMSKEP
                     IALQLRFLQTMREISSEHNTTTFLPVPIDLFTPFASKKQPPQP"
     gene            complement(3023..4303)
                     /locus_tag="VFO30_01105"
     CDS             complement(3023..4303)
                     /locus_tag="VFO30_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET9417913.1"
                     /translation="MKIALVGLFLWLACAAVSAAEKVALIRIDGAIGPATASYISRSI
                     DEARTQNAQSLIIQLNTPGGLLDSTQTIVQNLLGSPLPVIVYVAPTGGTATSAGCFIT
                     ISASVAAMAPATTIGAAHPVSIGGFSGGEQKPDDTMKQKLENFSVSYIEAIASKRKRN
                     VEWAKSAVRESASISAEKALELKVIDLIAVDLADLLKQLNGREVDGKKLNTTGAQVVE
                     IKMSAAEQIFQKLWRPEVMFVLMLIAIYGIIGELTTPGAILPGVVGAIALILALYLAA
                     VLPVNVTGLALIALALLLFICDVYAPTHGVLTMGGVISFLIGSLMLFNRADPLFHLSL
                     GYIIPATLVTVAFFLFVIGKGLRAQLLPVRSGAETMIGRTVAAVTPIDSRGGRVFVEG
                     EYWSAVSDTPIEKDRAVEITAVQGLTVQVKAKGE"
     gene            complement(4308..>5591)
                     /locus_tag="VFO30_01110"
     CDS             complement(4308..>5591)
                     /locus_tag="VFO30_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HET9417914.1"
                     /translation="PFAPVDQTRGYISMCKQLEQWLAEISGFAAVSLQPNAGSQGEYA
                     GLLAIREYHASRGEAHRNVCLIPTSAHGTNPASAVMAGLKVVPVATLKDGDVDLADLR
                     AKADEHARDLAALMVTYPSTHGVFEPTIREICEIVHAHGGQVYMDGANMNAQVGLCRP
                     GDYGADVCHLNLHKTFCIPHGGGGPGIGPIGVAEHLVPFLPGLPSIENREARIENRVG
                     AVAQAPFGSASILTISWMYIRMMGALGLTNATKIAILNANYIVKRLDPYFPVLFKGKR
                     GLVAHECIVDLRQWKSAGLEVEDVAKRLMDYGFHAPTVSFPVAGTMMIEPTESESKEE
                     LDRFCDAMISIHAEIDAVTTGKIDRQNNPLKNAPHTARQIASDKWDRPYSREQAAFPA
                     PWTREHKFWPAVARIDNVYGDRNLFCSCPPVEEFD"
BASE COUNT         1119 a         1655 c         1607 g         1210 t
ORIGIN      
        1 acgtttggcg ccgtgttctt tttcatctac tgccaattgc gctggggcca ctgggacatt
       61 tacatgctca cccaatcgtc cggctgggca atcgtccccg attacctcgc cgtgcttaaa
      121 ccgtccagtt accgctggct cgtgccgggg ctgaacgacc ccacggcgat gagtcagatg
      181 tcgatgacct tcggcgcgtt gttcttcctg ctgattgcga ttgttgaaat cgtcccggcc
      241 atccgccgca acacgaattg ggctgcgcgg atcggcatct acttttgcgc ggccgtcatt
      301 tattacatct cggtcagcgg cgtggccagc gtcgatatgg aaagcatgct gcgctacgaa
      361 ttttccgtgc atgcgttaat cgtgctggca ttactgcatt ttctgcgtca gtttgccaag
      421 ccacccgttt acgtgcgggc attcggcatg gcggccgtgg cgctggctgc cgtcgctgga
      481 ttgatgttgc aggggtggta tgtctggaat tttacgcgtg gcaactgggt tgcctaaaat
      541 gaaaacaact gtcgccatca tcggagccgg tccagcggga ttgaccgccg cgtacttact
      601 ttgcaaaaac gacgtcgatg ttgtcgtgct cgaagccgac ccggtttatg tcggcggtat
      661 ctcccgcacc gccacttaca aaggttttca tttcgatatc ggcggccacc gcttcttctc
      721 caaatccaaa gcggtcgaag atttctggac ggagattttg cctaacgaca tgctccatcg
      781 tccgcgctcg tcgcgcattt tttacggcgg caaatttttt acctatccgc tcaagccgtt
      841 cgaggccctc atcaaactcg gcgttttcca atccattctt tgcgttctct cctggctgaa
      901 agcgcggctc ttgcccgtcc gcgacccgcg caattttcaa gactgggtga gcaatcagtt
      961 tggccgtcgg ctcttcaaaa ttttcttcaa gagctacacc gaaaaggtct ggggcatgag
     1021 ctgcaaggaa atctcggccg actgggcggc gcaacgcatc aaaggtctct cgctcggcag
     1081 cgcgatcaag aacgcgctca tcccgcagcg ttacaatggc gaccggtcgc atgtgatcaa
     1141 gacgttgatc aactcatttc gttatccacg caaaggaccc ggcatgatgt gggaagcgtg
     1201 cgccgagaag gtgcaagcaa tggacggcca gattcacatg ggctgcaaag tcaccggctg
     1261 tgcttacgac gatgtaacat cgacatggac gctccggttc caagatagac aagctaacgt
     1321 gcagacgatc gaggccgatc acgtgatctc ctccgcgccg atgcgtgcgc ttgtgcgcgg
     1381 cctcaccccg cgcgtctccg agcgcacgtt gcgcgccgcc gacaggctga agtatcgcga
     1441 tttcctcacc gtcatgctga ttttaaagga gcgaaacaaa ttcgccgaca actggattta
     1501 catacacgac ccgagcgtga aagtcggccg ggtccagaac ttccgttcct ggtcacccga
     1561 gatggtgccc gatccggaca aagcctgtta cggcctggag tatttctgtt tcgaaggcga
     1621 tgggctttgg gaatcacgcg acgaagattt gatcgagctg ggtaaacgcg agctgatcaa
     1681 gatcggtctc gcccaggaag gcgatgtcgt ggacggcaca gtcgtacgtc agaaaaaagc
     1741 ctacccggtt tacgacgacg attacgcgat gcacgtcgct actattcgac aggagctgga
     1801 gcaacgttat ccaaacctgc acctggtcgg ccgcaacggc atgcacaagt acaacaacca
     1861 ggaccacgcc atgatgacgg cgatgttgtg cgtggaaaac attctcgcga atacgcggct
     1921 ctacgacctc tggcaggtca accaggacgc cgagtaccac gaagccggcg ccgctcccgc
     1981 cgaagaatca aacggcgccg ctttacgttt agttccaacg cgggtcgtcg cgacccccga
     2041 gctcgcgcca gaaggttgat tctgtagcgg cggtctatga ccgtcgcatt cataaatcgt
     2101 cgcgaacggg gctgaaggaa aaggtcagat ttgtcgcggc ggctgctttc ttgtcattcc
     2161 gagcgaagtc gaggaatctc ttactgtttt cggactggaa atcttgcagg tgtgacgagt
     2221 gaaacaaaca tcgctacggt tggggtggtt gctttttgct cgcgaatggc gtgaagaggt
     2281 cgatcggcac gggaagaaac gtcgtcgtgt tatgttcact ggaaatttcg cgcatggttt
     2341 ggagaaagcg cagttgcagc gctatcggtt ccttgctcat catcgcggcg gcctgcacca
     2401 ttttttcggc cgcttgaaac tcgccttcgg cgttgacgat cttggcgcgg cgctcgcgct
     2461 cggcttcggc ctgcttggcc atcgcgcgct tcatggtgtc gggcagcgcc acatctttga
     2521 cctcgaccgc cgtgactttg ataccccacg gttcggtttg ccggtcgatg atctcctgta
     2581 acttcaggtt gatcgactcg cgctgcgaaa gcaggtcatc caagggggct tggccgagca
     2641 cgctgcgcag cgtcgtctgc gacatcagcg aggtggcctt gagaaagttc tccactttca
     2701 cgaccgcggc ttgtggatcg accacgcgaa aataaacaac cgcatcgacc gtcaccggta
     2761 cgttatcgcg tgtcatcact tcctgtcgct cgacgctgat ggtcaccacg cgcaaatcca
     2821 tccgcaccat tcgatcgaca atcgggatca gcaggatcag ccccggtcct ttcgcgccga
     2881 gcagtttccc gagacgaaaa atcacgccgc gttcatactc gcgcaaaatc cggatcgcct
     2941 gcggcaggat gatcgcggcc aggatgatga ggggcagggc ccatctggtt aaccttagca
     3001 gtccttcaag cagtggcatc gcttattctc ccttggcttt tacttgcaca gtcaatcctt
     3061 gcacagccgt aatttcgacc gcgcggtctt tttcaatcgg cgtgtcactg acggcgctcc
     3121 aatattcgcc ttcgacaaaa acacgcccgc cgcgcgagtc gatcggcgtc acggcagcga
     3181 ccgttcgccc gatcatcgtc tccgcgccgg agcgcaccgg caggagctga gcgcgcaatc
     3241 ctttgccgat gacgaaaagg aaaaacgcta ccgtgacgag cgttgccgga atgatgtagc
     3301 cgagcgacaa atgaaagagc ggatcggccc ggttaaaaag catgagcgag ccgatcaaaa
     3361 acgaaatgac gccacccatc gtcaacacac cgtgcgtagg cgcgtagacg tcgcagatga
     3421 agagcagcag agcgagcgcg atcaaggcga gtccggtgac attcaccggc aggacggcgg
     3481 cgagatagag cgcgaggatc agcgcgatcg cgcccaccac tccgggcaaa atcgctccgg
     3541 gcgtcgtgag ttcgccgatg atcccgtaga tcgcgatgag catgagcacg aacatcacct
     3601 ccggccgcca cagcttttga aagatctgtt ccgccgccga catcttgatc tcgacgacct
     3661 gcgcgccagt cgtgttcagc ttttttccat ccacctcgcg cccgttcaac tgcttgagca
     3721 aatcggcaag gtcgacggcg attagatcga taacctttag ctcgagcgct ttctccgcgc
     3781 tgatcgatgc gctctcgcgc acggccgatt ttgcccactc cacgttccgc ttccgtttgc
     3841 tcgcgatcgc ttcgatgtaa ctcaccgaaa aattctcgag cttttgcttc atcgtgtcgt
     3901 ccggcttttg ttcgccaccg ctaaaaccgc cgatcgacac cggatgcgcg gcaccgatcg
     3961 ttgtcgccgg cgccatggcg gcgacgctgg ccgagatggt aatgaagcag ccggcgctcg
     4021 tggccgttcc gccggtcggc gcgacgtaaa cgatcaccgg caaaggggag ccaagcagat
     4081 tttgcacgat cgtttgcgtg gaatcgagca ggccgcccgg cgtgttcagt tggatgatga
     4141 gagactgcgc gttttgcgtg cgcgcttcgt caatgctgcg ggaaatgtaa cttgccgtcg
     4201 cgggaccgat cgctccatcg atcctgatca gcgcaacctt ctccgctgcc gataccgccg
     4261 cgcacgccag ccacagaaac aaacctacca gcgcgatttt cattagctca gtcgaattcc
     4321 tccaccggcg gacaggagca gaagaggttg cggtcgccgt agacgttgtc aatgcgggcg
     4381 acggcgggcc agaatttgtg ttcgcgtgtc cacggcgcgg gaaaggcggc ttgctcgcgc
     4441 gagtaggggc ggtcccattt gtccgatgcg atttgccgtg cggtgtgcgg cgcgtttttg
     4501 agcgggttgt tctgcctgtc gatcttgccg gtcgtgacgg catcgatttc ggcgtggatt
     4561 gaaatcatcg cgtcgcagaa acggtcgagc tcctctttcg attcgctctc ggtcggctcg
     4621 atcatcatcg tgccggcaac cggaaacgag acagtcggcg cgtgaaaacc gtaatccatt
     4681 aaacgtttcg ccacgtcttc gacttcgagt ccggcgctct tccattgccg aagatcgaca
     4741 atgcactcgt gcgcgacgag gccacgtttg cctttgaaca acaccggaaa atacgggtcg
     4801 agccgtttca cgatgtagtt cgcgttcagg atcgcgatct tcgtcgcatt cgttaggcca
     4861 agcgcgccca tcatgcggat gtacatccac gagatcgtca ggatgctggc gctgccgaag
     4921 ggagcttgcg caaccgcacc gacccgattc tcgattctcg cttctcgatt ctcgattgac
     4981 ggaagccctg gcaaaaatgg cacgaggtgc tcggccacgc cgatcgggcc gatgccgggg
     5041 ccgccgccgc cgtggggaat gcaaaacgtc ttgtgtaaat tgagatggca aacgtcggcg
     5101 ccgtaatcgc ccgggcgaca aaggccgacc tgcgcgttca tgttcgcgcc gtccatgtaa
     5161 acctggccgc cgtgcgcgtg cacgatctcg caaatctcgc ggatggttgg ttcgaacacg
     5221 ccatgcgtgc tcggatacgt gaccatgagc gcggcgagat cgcgcgcgtg ttcgtccgct
     5281 ttcgcgcgca aatcggcaag atcgacatcg ccatctttca acgtcgccac cggcaccact
     5341 ttgagtccgg ccatgaccgc gctggcgggg tttgtcccgt gggcagaagt tggaatcaaa
     5401 caaacgttgc gatgcgcttc gcctcgcgac gcatggtatt cgcggatcgc gagcagcccg
     5461 gcgtactcgc cttgcgagcc ggcgttcggt tgcaacgaga cagctgcgaa cccggatatc
     5521 tcggcgagcc attgttcgag ttgcttgcac atgctgatgt agccgcgggt ctgatccacc
     5581 ggcgcaaacg g
//
LOCUS       DASTDG010000032         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_903026, whole genome shotgun sequence.
ACCESSION   DASTDG010000032 DASTDG010000000
VERSION     DASTDG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903026"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            729..1319
                     /gene="infC"
                     /locus_tag="VFO30_01115"
     CDS             729..1319
                     /gene="infC"
                     /locus_tag="VFO30_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9417915.1"
                     /translation="MQPQFRVNGRIRAREVRVVLGSTGQQLGVMKLSDALREAQNRGL
                     DLVEVAPTASPPVCKIVSLGKFRYDISKQEKDRKPSGAKLKEIKFRVNIDEHDYLTKI
                     RHGEEFLDRGNKVRVQLQFRGREMAHQEFGLQLMEKVRNDLSGMAQVEMEPKITGRNV
                     TMTLSPLPANRRKRKFAPPPKTPAPAESQNGSKSAS"
     gene            complement(1316..2170)
                     /locus_tag="VFO30_01120"
     CDS             complement(1316..2170)
                     /locus_tag="VFO30_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET9417916.1"
                     /translation="MLRVLFLGDVVGAPGRNAVIARLPELKEQQGIDFTVVNGENAAG
                     GRGITGKITIELLRAGVSVITTGDHIWDQKDILSFLDLEPRLLRPINYPAGAPGHGSI
                     VLETPKAKVAVINVQARTFMQPILENPFRALDEAIGALRQETFNIIVDVHGETTSEKI
                     AIGRFLDGKASAVIGTHTHVQTADEQIFPGGTAFLCDAGMCGPVNSILGRAIEPILQR
                     FISNLPASFPVATGEVRLRGALIDIDESSGRALRITRVDEAAPLTEAIRPELVQEHAA
                     GDDAAAAS"
     gene            2262..3497
                     /locus_tag="VFO30_01125"
     CDS             2262..3497
                     /locus_tag="VFO30_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417917.1"
                     /translation="MSIFCSNSDAADNLTVLGSKPRWTVLEHYQQTITRDEFTHLIND
                     IYCTHGRADDLIEIDNDSARILKNRAAQTFFTLRFANDDASRERVPRLWRPAKSLPPA
                     AAEKPLAQLRIALDPGHLGGEWAKMEERWLRVGDSRPVTEGDLTLKVARILAPQLRKL
                     GAKVLFVRNGNEPVTPMRPDDFKQLARKILIKNGVPQPRPDVLDPNDPAKEQTIRWQS
                     EILFYRFSEIRHRAVLVNTKLHPDIVLCLHFNAEPWGDPNSPTLTDVNHLHLLVNGSY
                     SQEELEFDDERFEMVRRLLSRAFDEELPAADAVATAMAQATALPPYQYPTTLTTNKVG
                     TSGYVYARNLLATRLYRCPVVYCEPYVMNGKETFARIQAGDYEGTREINGVQRKSIFR
                     EYADSVAQGLAEYYRNVRK"
     gene            complement(3560..>4054)
                     /locus_tag="VFO30_01130"
     CDS             complement(3560..>4054)
                     /locus_tag="VFO30_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9417918.1"
                     /translation="FDEVAQAAGWREASETLALQQLIKRNIEIKARVVAKDERDKTGE
                     RALLNFGHTVGHAIERAGDYRQLLHGEAISLGIVAACTISIERAGLPREQRDRVVDLL
                     RRFELPVRLPADFPRGKIFDALKFDKKFEHGEVRFVVISRIGSAFLSRDVTLDDIRES
                     VENL"
BASE COUNT          897 a         1177 c         1151 g          829 t
ORIGIN      
        1 acgctcgacg cgatgctcga tgatgccgag gcttacgtct catataaagc gcatttcggc
       61 gacccgccga caagttgatc ggattgaaga tcggcgttgc gattgctaaa ctacaccgtg
      121 aatccggaag aaattcgtgc ggatcatgac cggcgatcgc gttcatgatc aacgcggacc
      181 cgcgagactt gctcgatcat tacggccgga cacgcgggga agcctcagct gttgccagaa
      241 aataataaac atcaggcaga agttgcgagc aataccgttt gttccgtttc gcgtccacac
      301 gagtgacggt aaacaccttg acgtgaacca tcccgaaatg gcaatgctta ctcgaatggc
      361 gctcctggtt gcgcgtcctg tttctgatcc tacccagcag attcctgctc actacgattc
      421 ggtttcgccg ttgcacatcg tgcggcttga gccgcttgtt cctgcctgac tgctcgtgct
      481 caattcggcg cgccacctgc tgtgtcagtg caagatcgac aatcatagat tgatcttgcc
      541 agcgttagaa atgttgctta caacttcccc tcggctcgcg aattgcgggc ccgtcgacgc
      601 ggtcgtcgca gccgcgccga ggctgagtgc gtcagtgttt tagcgaactg aaatgctgat
      661 ttttgcgacg actgaaatga cggcgttgcg gtgcactccc gtttcgccgg cccggagatc
      721 aatttaatct gcaaccgcaa tttcgtgtaa acggccggat tcgcgcccgc gaagtccggg
      781 ttgttttagg ttcgaccggc cagcaactcg gcgtcatgaa attgtcggac gcgctgcgtg
      841 aagcgcaaaa ccgcgggttg gatttggtcg aagtcgcgcc aaccgcaagc ccgccggttt
      901 gcaaaatcgt ctcgctcggc aaatttcgtt acgacatttc caaacaagag aaggacagga
      961 agccatcggg cgcgaagctc aaggagatca aatttcgcgt caacatcgac gaacacgatt
     1021 acctgacgaa aatccgtcac ggcgaggagt tccttgaccg cggcaacaag gtgcgggtgc
     1081 aactgcagtt ccgcggccgc gaaatggcgc accaggaatt tggtctccag ctgatggaga
     1141 aggtgcgcaa cgatctgtct ggtatggcgc aggtggaaat ggagccgaag atcaccgggc
     1201 gcaacgtcac gatgacgttg tcgccgctac cggcaaatcg gcgcaaacgg aagttcgcgc
     1261 cgccgccgaa aacgccagcg ccagccgaat cgcaaaacgg cagtaaatcg gcgagctaac
     1321 tcgccgcagc tgcatcgtct ccggccgcgt gctcttgcac cagttcgggt cgtattgcct
     1381 cggtcaacgg ggcagcttca tcaacgcgcg taattcgaag tgcacgcccg cttgattcat
     1441 cgatgtcgat caaagctccg cgcaaccgga cctcgccagt cgcgacggga aatgatgccg
     1501 gtaaattcga gatgaagcgc tgcagaatcg gctcaattgc gcggccgaga atagaattga
     1561 cggggccgca catacccgcg tcgcacaaga atgcggtgcc gcccgggaag atttgttcat
     1621 cggccgtttg cacgtgcgtg tgtgtgccga tcacggcgga agctttgccg tcgagaaaac
     1681 ggccgatagc gattttctcg ctcgtcgttt cgccgtgaac atcgacgatg atgttgaaag
     1741 tctcctggcg cagcgcgccg attgcttcgt caagcgcgcg aaacggattt tccaagatcg
     1801 gctgcataaa ggtgcgcgcc tgcacgttga ttactgcgac cttagctttc ggcgtctcga
     1861 gaacgataga accgtggcca ggtgcgccgg ccgggtaatt aatcgggcgc agcaggcgag
     1921 gctcgaggtc gagaaaactt aaaatatctt tctggtccca aatgtgatcg ccagtcgtga
     1981 taacagaaac gccggcgcgc aaaagctcga ttgtgatttt gcccgtaata ccgcgcccgc
     2041 cagccgcgtt ttccccgttg acgacggtga aatcgatgcc ctgctgttcc ttcagctcgg
     2101 gcaaacgcgc aataacggca ttgcggccgg gtgcgcccac cacgtcgccg aggaaaagta
     2161 cccgtagcat tcggcgagca tcgtattaaa agagagcgaa atccaaatcg atgttctgcg
     2221 gcaggcattc atggcaatgc gcaatcctcg gcctctcgtt gttgtcgata ttctgtagca
     2281 acagcgacgc ggcggacaac ctaactgtcc tcggcagcaa gccgcgatgg acggtgctcg
     2341 agcactacca gcaaacaatt acgcgcgacg agttcacgca cctcatcaat gacatttatt
     2401 gcacgcacgg ccgcgctgac gacctgatcg agatcgacaa tgattcggcg cgcatcctga
     2461 agaatcgcgc cgcgcaaact ttcttcacgc tgcgcttcgc gaacgacgat gcctcacgcg
     2521 agcgcgtgcc gcgtctctgg cggccggcaa aatcgctgcc gcctgccgct gcagagaagc
     2581 cgctggcgca gctgcggatt gctctcgacc cgggtcatct cggcggggag tgggcgaaaa
     2641 tggaggagcg ctggttgcgc gtcggcgatt cacgcccggt gaccgagggc gatctaacgt
     2701 tgaaagttgc gcgcattcta gcgccgcagt tgcgcaagct tggagccaaa gtgctcttcg
     2761 ttcgcaacgg caatgagccg gtcactccga tgcgcccgga tgacttcaag caactcgcca
     2821 ggaaaatcct gatcaaaaat ggtgtgccgc aaccgcggcc agatgttctc gaccctaacg
     2881 acccagcaaa agagcagacc atccgctggc aaagcgagat tttgttttat cgcttcagcg
     2941 aaattcggca tcgcgccgtg ctcgtgaaca cgaagttaca tcccgatatt gtgctctgtc
     3001 tgcatttcaa tgccgagcca tggggcgatc caaatagccc aacgttgacg gacgtgaacc
     3061 atctccattt gctcgtgaac ggatcgtatt cgcaagaaga gctcgagttc gacgacgagc
     3121 gcttcgaaat ggtgcggcgg ctgctgagcc gcgcgttcga cgaagaatta cccgctgccg
     3181 atgcggtcgc gaccgcgatg gcgcaggcga ccgctctacc gccgtatcaa tacccgacca
     3241 cgctgacaac gaacaaagtt gggacgagtg gctacgtcta cgcgcgcaat ctgctcgcga
     3301 cgcgactcta tcgctgccca gtcgtctact gcgagccgta cgtcatgaac ggcaaagaaa
     3361 cattcgcgcg cattcaggct ggcgattacg aaggcacgcg cgagatcaat ggcgtccagc
     3421 gcaaaagcat tttccgcgaa tacgccgaca gcgtcgcgca aggtttggcg gagtactatc
     3481 ggaacgtgcg aaaataactt cttctgtagc ggcggtctgt gaccgtcgga cgagttaatg
     3541 cgacgctcat agagcgccgc tacagattct ccaccgattc gcgaatgtca tcgagggtca
     3601 cgtcacgcga caaaaaggcc gagccgattc tcgagatgac gacgaagcga acttccccgt
     3661 gctcgaactt tttgtcgaac ttaagcgcat cgaaaatttt gccacgtgga aaatccgccg
     3721 gcaatcgcac cggcagttcg aatcggcgga gaagatcgac aactctatcg cgttgttcgc
     3781 gcggcagacc ggctctttcg attgaaatcg tgcacgctgc aactattcca agactgattg
     3841 cctcgccgtg cagcaactgg cgataatcgc cggcccgctc gatcgcgtga ccaacggtat
     3901 gcccgaaatt gagcagcgcg cgttcgccgg tcttatcccg ctcatctttc gctacgactc
     3961 gcgccttgat ctcaatgttg cgcttgatca gttgttgtag ggcaagcgtc tcgcttgcct
     4021 ctcgccagcc ggcggcttgc gctacttcgt cgaa
//
LOCUS       DASTDG010000033         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1001767, whole genome shotgun sequence.
ACCESSION   DASTDG010000033 DASTDG010000000
VERSION     DASTDG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001767"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VFO30_01135"
     CDS             complement(<1..1046)
                     /locus_tag="VFO30_01135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9417919.1"
                     /translation="MERSNSRSTSAAGVSEGSATCEQCGATTRVGEGRCLTCYLKEGL
                     EAEGEPSAEVFEGVLAEADVPDTQWRLGNYEILEEIGRGGMGVIYRARQRHSRRIVAL
                     KRVLTYHADSHETLARFRREAEAAASLDHPNILPIYEVSETDDGIPLFSMKLAAGGSL
                     RNAAEAFRSEPRECVRLLVKVARAIDYAHAHGILHRDLQPGNILLDARGEPMVSDFGL
                     AKWLNDESDLTQTLTTFGTPGYIAPEQGEGADFSLAADIYGLGAILFNLLTGRPPFTG
                     ANALSVIRQAAATAAPKLRAFAPSLGRDLETIIARCLERDPNARYQTAGALAEDLERW
                     LEGRPILARRVRAPA"
     gene            complement(1046..1816)
                     /locus_tag="VFO30_01140"
     CDS             complement(1046..1816)
                     /locus_tag="VFO30_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9417920.1"
                     /translation="MSRATEPVNSAEPRVFATTRWSLILAAGDSDSDEQKARQALTEL
                     CRTYWRPVFLFVSRRGYPVPEAQDITQDFFVMILESKWLQQADEHRGRFRSFLLKSLQ
                     NFLSHAREREGAVKRGGRMEFISWDEWMAEAPSKLSVPSQRLDSLPPEHLFDLRWAAT
                     VVEHALQRLREECEGKGRLRLFETLSAHLTAERADVSYATLAARLGIAETAVKKQLHN
                     LRQRYRWLLRDEVAQTVENPADIDDEIRHLCAVLAETA"
BASE COUNT          412 a          641 c          569 g          424 t
ORIGIN      
        1 gcgggagcgc gtactcgtcg ggcaagtatc ggccgtcctt cgagccagcg ctcgaggtcc
       61 tcggccagcg cgccagcggt ctgatatcgc gcgttcggat cgcgctcgag acaacgcgcg
      121 atgatcgttt ccaagtcgcg accaagcgag ggcgcgaacg cgcgcaactt cggcgccgct
      181 gttgcggctg cctgtcgaat caccgaaagc gcattcgcgc cggtgaacgg cggccgacca
      241 gtcaacagat tgaaaagaat cgcgccgaga ccgtaaatat ctgcggccag actaaagtcg
      301 gccccttccc cctgttccgg agcgatgtag cccggagttc caaatgtcgt cagcgtttgg
      361 gtaaggtcgc tctcgtcatt aagccatttg gccaggccga agtcgctcac catcggttcg
      421 ccgcgcgcgt ccagcaaaat gtttccgggc tggagatcgc ggtgaagaat tccatgtgcg
      481 tgcgcgtagt cgatcgcacg cgcgactttc actaacagcc ggacacattc gcgcggttcg
      541 ctccgaaaag cctccgcggc attacgcaaa cttccgcccg cggcgagttt catgctgaaa
      601 agcggaatcc cgtcgtccgt ttcgctgacc tcgtaaatgg gaagaatatt tggatgatcg
      661 agactagcgg ctgcttccgc ttcgcgccgg aaccgcgcca gcgtctcgtg cgagtcagcg
      721 tggtaagtga gcacccgttt caacgccacg atgcgccgcg aatggcgctg ccgtgcgcgg
      781 taaatcacac ccatcccgcc gcgcccgatc tcctccagaa tctcgtaatt gcccagtcgc
      841 cattgggtgt ctggcacgtc cgcttcggcg agcacgccct cgaaaacttc cgccgaaggt
      901 tcgccttccg cttccagtcc ttctttgaga tagcaagtca gacagcggcc ttcgccgacg
      961 cgcgtggtcg cgccgcattg ctcgcatgtc gccgaacctt cgcttacgcc cgcggccgag
     1021 gtggagcgac tattcgatct ctccatcacg ccgtctccgc gagcacggcg cagagatgac
     1081 ggatctcgtc gtcaatgtcg gccggatttt ccactgtctg cgcgacttca tcgcgcagga
     1141 gccaccggta acgctgccgc aggttgtgga gttgtttttt gaccgctgtc tcggcgattc
     1201 ccaaacgcgc cgcgagagtg gcatacgaca catcggcgcg ttccgcggtt agatgtgcgc
     1261 tcaatgtttc gaacagccgg agccggcctt tgccctcgca ttcctcgcgc aagcgctgca
     1321 acgcatgctc gaccaccgtc gccgcccagc gcaagtcgaa caggtgttcc ggcgggagcg
     1381 aatccagcct ttgcgacggg accgacaact tcgacggcgc ttcggccatc cattcatccc
     1441 acgaaataaa ttccatccgc ccgcctcgtt ttactgcgcc ctctctttct ctcgcgtgac
     1501 tgagaaaatt ctgcagtgac ttcaagagga acgatcggaa acgtccgcga tgttcgtccg
     1561 cctgctgcag ccacttgctc tccaggatca tgacaaaaaa gtcttgggta atgtcttgtg
     1621 cctccggtac cggataaccg cgacggctga cgaacaggaa aacggggcgc caataagttc
     1681 ggcagagctc ggtcagtgct tggcgtgcct tttgttcatc ggaatccgag tcaccggcgg
     1741 caagaatcaa gctccagcgc gtggtcgcaa acacgcgcgg ttcagctgaa ttgaccggtt
     1801 ctgtcgcccg actcacgaga ggagcagcct ggcaaaattc agcttctaaa acaagcgtaa
     1861 aaagccgcca cctgtcggcg gttttccctc gagccgttgg ccggaataat caatcaggaa
     1921 cttgtccctt catttcacta ggaactttgc caggccattc cgttagaatg cttggtgaaa
     1981 ggcaaaacat gaaaacatta aaactcctga aaccaatagt aagctcctca ctttttgcgt
     2041 gtggat
//
LOCUS       DASTDG010000034         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_667036, whole genome shotgun sequence.
ACCESSION   DASTDG010000034 DASTDG010000000
VERSION     DASTDG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667036"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFO30_01145"
     CDS             <1..203
                     /locus_tag="VFO30_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417921.1"
                     /translation="GGGTHHLQVSWLFECTDNTGTAVPCTDVYTANTAGACAGPGTVT
                     VTQTKAFNQSGGITIGPVSGAP"
     gene            complement(221..1243)
                     /locus_tag="VFO30_01150"
     CDS             complement(221..1243)
                     /locus_tag="VFO30_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9417922.1"
                     /translation="MQLQRNEVMTGLLVVGTIAVITAVLILLGAPGLFRPLVTYKIYF
                     DNASGIKLGAPVMLAGRKIGQVQKLYSPVSVEEDRRAQEVAAAARVPDPNAKPPPADG
                     KPKFEVRVDVQVDRSARVYRDARTRLMQLGLLGEMAIDITQGTEASGRAHDNEIFAGE
                     RTPDFSEAASKLLEVIKPVAAEATMTMKDLEVTAQNINHITDQNSELNLALTQFKTFG
                     EHLVDLTAPEGPLTQSLENVRKLSDSLTEDNNIEVTLRNFRDSSDKLKSTMRELEPVG
                     RNIAEFSETVKTQPWRLIWPSTKKSPNVSPTPGEQTITVRKRARSQQSTKAQPSPSAA
                     RSGGSR"
     gene            complement(1224..2015)
                     /locus_tag="VFO30_01155"
     CDS             complement(1224..2015)
                     /locus_tag="VFO30_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9417923.1"
                     /translation="MSTQEPDVVELRDVHMQFEEKKVLDGISLEVHLQDRLVIMGQSG
                     SGKSTILRLILGILQPISGSIFFKQFEITRLKRRKLQQVRRHIGMVYQYSALLSSRNV
                     RDNIALPLEELTDKSPEEIDEIVDQKLELVGMQDSKDLMPSELSGGMRKRVSLARALV
                     LEPELILFDEPAAGLDPVIASVIDELIIDLTERSKVTSIIVTHEMGSAFRIGTRMAML
                     YQGQIIEDAAPEEFKRSTNPVVHQFLSGSTEGPILEGTQDAIAAK"
     gene            complement(2012..2830)
                     /locus_tag="VFO30_01160"
     CDS             complement(2012..2830)
                     /locus_tag="VFO30_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417924.1"
                     /translation="MPQPNEAVMTAPGLQFARNFFREVGSLFWFIANTINETMERLQS
                     GRLPFRAASFFRHTQRSGVDSVPLVSLVSFFLGLTMALLTGYQLQRFGTERLIPGLVS
                     IAFTRELGPLITGIMLAARIGAAFTAELGTMQVSEEVEAIEAMGIGPLRFLVAPRMLA
                     LFFLMPCLSTVSNIAAIFASSLVSRAYFSIAFVYFLDLVRDALLIRDIITGVLKSFLF
                     ALLIGAIACYRGLTVRGGAAGVGTATTSSVVTAVTTVIGFDTLYNIVYIIFYPT"
     gene            3205..4041
                     /locus_tag="VFO30_01165"
     CDS             3205..4041
                     /locus_tag="VFO30_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET9417925.1"
                     /translation="MRPRSPAFAFIVFVLILFLVLRSARVIPAGHVGIVDLFGKVRAE
                     ALPSGLHLVNPLANVHRMSIQTSEVKETMDTPSSEGLIVHLDVSILYHLDTGKAPEVY
                     RTIGYDYETVLVKPNVRSAVREVTSSYEAKALYSPEREKMSAEINQHIVAAIQPRGVV
                     VERMLLRDVALPARLQQAIQEKLSAEQEAARMQFVLLKEKQEAERKKIEAEGISNFQK
                     IVTEGINENLLKWKGIEATKELALSGNAKVVVIGAGKEGLPIILGGDYAPPAPHPSPP
                     GR"
     gene            4166..>4357
                     /locus_tag="VFO30_01170"
     CDS             4166..>4357
                     /locus_tag="VFO30_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET9417926.1"
                     /translation="MPKSQNKIGLVVHGGAGTIERNEMTPEKERAFHEGLERALKAGY
                     EILQPGGSSLDATEAAVRVL"
BASE COUNT          911 a         1251 c         1303 g          892 t
ORIGIN      
        1 tcggtggcgg tacgcatcac ctccaggttt cgtggctatt cgaatgcacc gataacaccg
       61 gcaccgctgt cccctgcacc gatgtttaca ccgccaacac cgccggcgcg tgtgctgggc
      121 cgggcaccgt cacggtcacc cagacgaaag ccttcaacca atccggcggc atcactattg
      181 ggcccgtaag cggcgcaccc tgacagaaaa tcagagcacg ctagcgcgac ccacctgagc
      241 gcgctgcgct tgggctcggc tgcgctttcg tgctttgctg cgatcgcgcc cttttgcgga
      301 ccgtgatcgt ctgttcgccg ggcgtgggtg acacgtttgg tgatttcttc gtgctcggcc
      361 aaatcaggcg ccacggctgt gttttcactg tctcgctgaa ctcggcaatg ttgcggccga
      421 ccggctcgag ttcgcgcatg gtcgatttca atttgtccga ggagtcacga aaattgcgca
      481 gcgtgacttc gatattgttg tcttcggtta agctgtcgct gagtttgcgc acgttctcca
      541 gcgactgcgt caacgggcct tccggcgcgg taagatcgac aaggtgttcg ccgaaggttt
      601 tgaactgggt gagggcgaga ttgagctcgg agttttgatc ggtgatgtga ttgatgtttt
      661 gcgccgtgac ttcgagatct ttcatcgtca tggtcgcctc ggccgcgacc ggcttaatca
      721 cttcgagcag ctttgaggcg gcctcgctga agtccggcgt gcgttcgccg gcgaaaattt
      781 cgttgtcgtg cgcgcgcccg gacgcttccg tgccctgggt aatatcaatg gccatttcgc
      841 ctaacaaacc gagctgcatc agccgcgttc tcgcatcgcg ataaacgcgc gcgctcctgt
      901 ccacctgaac gtcgacgcgc acctcgaatt ttggttttcc atcggccggc ggcggctttg
      961 cgttcggatc gggtacccgc gccgccgcgg cgacttcttg tgcgcgccgg tcttcttcta
     1021 ccgagacggg cgaatatagt ttttgcacct ggccgatttt gcggccggcg agcatcaccg
     1081 gtgcgccgag tttaataccg ctcgcgttat cgaaataaat tttgtaagtg acaagggggc
     1141 ggaacagccc gggcgcgccg agcaggatca acacggcggt aatgaccgcg atcgtgccga
     1201 ccacgagcag gccggtcatt acttcatttc gctgcaattg catcttgcgt tccttccaga
     1261 atcgggcctt cggtgctgcc gctcaagaac tgatgcacca ccgggttggt tgatcgcttg
     1321 aattcctcgg gcgcggcgtc ctcaatgatt tgtccttggt aaagcatagc cattcgcgtg
     1381 ccgatgcgaa aggcgctgcc catctcgtgg gtgacaatga tggatgtcac cttggatctt
     1441 tccgtgagat cgatgatcaa ctcatcgatg actgaggcga tcacggggtc gagaccagcc
     1501 gccggttcgt cgaacaggat cagctcgggc tcgagcacaa gcgcgcgggc gaggctgacg
     1561 cgcttgcgca ttccgccgct caattccgat ggcatcaggt ccttggaatc ttgcatcccg
     1621 acgagctcga gtttttgatc gacaatctcg tcgatttctt cgggcgattt gtcggtcagt
     1681 tcttccagcg gcaacgcgat gttgtcgcgc acgttacgcg aactgagcag ggcggagtat
     1741 tgataaacca tgccgatgtg acgccggacc tgttgcagtt tgcgccgctt caggcgcgtg
     1801 atctcgaatt gcttgaagaa aatcgagccg gagatcggct gcaaaatccc gaggatgagc
     1861 cgcagaatgg tgctcttgcc gctgccgctt tggcccatga tgacgagccg gtcctgcaga
     1921 tggacttcga gcgaaatccc gtcgagcacc ttcttttcct cgaactgcat atgcacatcg
     1981 cgcagctcga cgacgtcggg ttcctgggtg ctcacgtcgg gtaaaagatg atgtaaacaa
     2041 tgttgtagag cgtgtcgaag ccgatcacgg tcgtgacggc ggtgactacg ctcgaagtgg
     2101 tcgcagtgcc gacgcccgct gcgccaccgc gcaccgtgag gccgcggtaa caggcaatcg
     2161 cgccgatgag caacgcaaag aggaaacttt tcagcacacc ggtgatgatg tcgcgaatga
     2221 gcagcgcatc ccgcaccaaa tcgagaaagt aaacaaacgc gatgctgaaa taagcgcgcg
     2281 agacaaggct gctcgcgaat atggcggcaa tgttcgagac cgtggagaga cacggcataa
     2341 ggaagaacaa cgcgagcatg cgcggagcga caagaaaccg cagcggtccg atgcccattg
     2401 cctcgatcgc ttcgacttct tcagaaacct gcatcgtgcc gagttccgcg gtaaacgccg
     2461 cgccaatccg cgccgccagc atgatgccgg tgatgagcgg gccgagctcg cgtgtaaacg
     2521 cgatcgagac caagccggga atcaatcgtt ctgtcccgaa gcgctgcaat tggtagccgg
     2581 tcaacaacgc catcgtcagg ccgagaaaga atgaaacgag actgaccagc ggcaccgagt
     2641 cgacgccgga gcgctgcgtg tgccggaaaa agctcgccgc acgaaacggc aaccggccgc
     2701 tctgcagacg ctccatcgtc tcgttgatgg tgttcgcgat gaaccagaac aagctgccca
     2761 cttcgcgaaa aaaattgcgc gcgaattgga gacccggcgc ggtcatgacc gcctcgttcg
     2821 gttgcggcat tcgggacatt gcctcagttc gcggagcgga aaaggacaaa atccctccag
     2881 tcggttcggc cagaatgaca gctgccgcac ggctttcagc gtgccacaat tggtatagga
     2941 gagatccttc gcttccgctc aggatgacag cgcgtgtgga gatgacgctg aaacttacgg
     3001 gcgcgacacg cggctccgcg gtcattccga gcgaagcgag gaacctccca atcggagtga
     3061 tcggcacgct gggtacgtta tctgatcgaa ggccagcggg gcatccttcg cttccgctca
     3121 ggatgacggc attgatggtg tgcgcgtcga gtttgcggca aaaagcttcg acagctttga
     3181 gcccggttgc ttaagctggc gcgcgtgaga ccgaggagtc ccgctttcgc gtttattgtt
     3241 ttcgttctca ttctgtttct ggtgctgcgc tcggcgcgag tgatcccggc cgggcacgtg
     3301 ggaattgtcg atcttttcgg caaagtgcga gccgaagcgc tgccgtccgg cctgcatctg
     3361 gtcaatccgc tggccaacgt ccatcgcatg tcgatccaaa ccagcgaagt aaaggaaacg
     3421 atggacacac cttcgagcga aggtctgatc gttcacctcg atgtcagcat tctttatcac
     3481 ctcgataccg gtaaagcgcc ggaagtttat cgtaccatcg gctacgacta cgagaccgtg
     3541 ctggtgaagc cgaacgtgcg cagcgccgtg cgcgaagtga ccagctccta cgaagccaag
     3601 gcgctctatt cgccggaacg cgaaaagatg agcgcggaaa ttaatcaaca cattgtcgct
     3661 gcgatccaac cccgcggtgt cgtcgtggag cggatgttgt tgcgcgatgt cgccctgcct
     3721 gcgcgcttgc agcaagccat tcaggaaaaa ttgagcgccg aacaggaagc cgcgcgcatg
     3781 cagttcgtgc tactcaagga aaaacaggaa gccgagcgca aaaaaattga agccgaaggc
     3841 atctccaatt ttcagaaaat tgttaccgaa ggcatcaacg agaacctgct gaagtggaaa
     3901 ggaattgaag cgaccaagga actcgccctc agtggtaacg ccaaagtggt cgtgattggc
     3961 gcgggcaagg aagggttgcc gattatcctc ggcggcgact acgccccgcc cgcgccgcat
     4021 ccgtcaccgc ccggccgcta agatcgacaa ccgtaagatc gacatcgtga aaagaccgtg
     4081 cgcaaccaat tgcttgttcg cggctgcgct ttttagtgca acctcgctcg cgctcgcaca
     4141 gcaacctacc gcatcggaat caagcatgcc caagtcgcaa aataagatcg gactcgttgt
     4201 ccacggcggc gctgggacga ttgagcgcaa tgagatgacg ccggaaaagg agcgcgcatt
     4261 tcacgaggga ttggagcggg cgctgaaagc cgggtatgaa attctgcagc ccggcggctc
     4321 cagtctggat gcgaccgaag cagcggtgcg cgtgctc
//
LOCUS       DASTDG010000035         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_884502, whole genome shotgun sequence.
ACCESSION   DASTDG010000035 DASTDG010000000
VERSION     DASTDG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884502"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1056)
                     /locus_tag="VFO30_01175"
     CDS             complement(172..1056)
                     /locus_tag="VFO30_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HET9417927.1"
                     /translation="MKVTRIHAPDFDLEKTLNSGQVFHWEKIDNGFIGTIGDLPVYVE
                     QREGVLKVASASSRCAAEHGQDAHATIARYFALDHPLAEICASLPNDSVMNAARAFSR
                     GLRIIRQPKWECLATFICSAMKQVAHSRQISQKLRARFGEPREIDNYVVHTFPSAQRI
                     ALVSEKELRDCALGYRARNLLATARLVATGDVDLEKMSALSDAELQAALCQLPGVGAK
                     VANCVMLFAYERLRAFPIDVWIERVLRERYFAKKRKVTPRRLRQFTESYFGEHGGYAQ
                     QYLFHHARLTRHLSAPKA"
     gene            1158..2759
                     /locus_tag="VFO30_01180"
     CDS             1158..2759
                     /locus_tag="VFO30_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET9417928.1"
                     /translation="MNGRLAIDSGVLLLYFVIIIAIGLFMGRRERDLEDFALGRRQIP
                     WWAVLASIIAAETSAGTFFGAPGEGFALRNYTYIQLAFGTIIARVLASYIFIKPYYDY
                     KVFSIYEYLTARFGVGTKNAASAVFMFTRVLASGARLYVAAIALVLAYEMIRGARPGQ
                     TETLFIYIGATIAIVVLTAIYTTIGGIKAVVWTDFIQASIMMGSALTALGILYFAIPG
                     GLHEIVQRHGGFHLSDVITTGLDPAKSGWDKIKGMFEIEYTIFAGLIGSTFITMATHG
                     TDQDMVQRMLTASDIRRSRRSLILSGLADIPIALTFLTIGLLLWVYYQTHFDPNLPKS
                     PNETFCHFILYEMPVGLRGLLIAGIFATAMGSLSTAINALATSFTRDWYEPYINPHAT
                     GEQSLRTVRWATVGFSILMIIVASLTSYFVIVMPNVRIIPIVLGIYGYTYGSVLGIFL
                     AGMLTKTRGNSRGNIIAMITGFIVVSILSGLPNNIAGIFGAKFYTQPTWLPLMEFPWW
                     ICFGTIVTFSVAILFRTGHEHHLPI"
     gene            3121..3354
                     /locus_tag="VFO30_01185"
     CDS             3121..3354
                     /locus_tag="VFO30_01185"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HET9417929.1"
                     /translation="MIKEHDRVVLTDDVPSERLKAGDIGTIVHIHRGGEAYEVEFICL
                     DGETIGVVTLKASQVRPAQSREIAHARELARNP"
     gene            3379..3687
                     /locus_tag="VFO30_01190"
     CDS             3379..3687
                     /locus_tag="VFO30_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9417930.1"
                     /translation="MTDKCNGCKFTDCVEVCPVACFYELDSQVVIHPEDCIDCMACVD
                     VCPVHAIYAEDDVPPEFAKDVEFNAVQSRQLKDSGAPAITARKEPLPGALERKTALGY
                     "
     gene            3929..>4213
                     /locus_tag="VFO30_01195"
     CDS             3929..>4213
                     /locus_tag="VFO30_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417931.1"
                     /translation="MPAPRVIGDLVERFEQQGDAYKSGQYNEAQLRKECVDPMFKALG
                     WDMDNISGYAKAYKDVIHEDAIKIGGATKAPDYPPTKLTKIDTIEWSRWSS"
BASE COUNT          925 a         1252 c         1113 g          923 t
ORIGIN      
        1 gccgactgcc gccagtccga acgcgacgag cagatgaatg agcagcgccg cgacaaacgc
       61 ccagaaaact ttgcggaact cgtgtttccg atccagcaac gtgtcgcccg gctgggcaaa
      121 catcatctcg gcagtcgtca tgcttccctc taaaagagtc gcaacattat ctcacgcctt
      181 cggcgctgac aaatggcggg tcaggcgcgc atgatgaaac aagtattgct gggcataacc
      241 accgtgctca ccaaaatacg actccgtaaa ctggcgcagc cgtcgcggtg tgaccttccg
      301 cttcttcgca aaatatcgct cccgcaggac gcgctcgatc cagacgtcaa tcggaaaagc
      361 gcgcaggcgt tcgtaggcga agagcatcac acaattagca accttcgcgc cgacgcccgg
      421 cagctggcac agcgccgcct gaagctcggc atcggaaagc gcgctcatct tttcaagatc
      481 gacatcgccg gtggcaacta gccgcgccgt cgccagcagg ttcctcgcgc gataacccag
      541 cgcgcagtcg cgcaactctt tttctgagac gagggcgatt cgttgcgctg acggaaacgt
      601 gtgaacaaca tagttgtcga tctcgcgtgg ttcgccgaaa cgcgcgcgca gtttttgtga
      661 gatctgccga ctgtgcgcca cttgcttcat cgcggagcaa ataaacgtcg ccaggcattc
      721 ccacttcggt tgccgaatga tgcgcagccc gcgagaaaac gcgcgcgccg cattcatcac
      781 actgtcgtta ggcaaagatg cgcaaatctc cgcgagcgga tgatcgagcg cgaaataacg
      841 cgcgatcgtg gcatgcgcat cctgcccatg ttctgccgca caccggctgg aagccgatgc
      901 cacttttagc acaccctctc gttgctctac ataaaccggc aaatcgccaa tcgttcctat
      961 aaatccattg tcgatctttt cccaatggaa gacttggccg gagttcaacg tcttttccaa
     1021 atcaaaatcc ggcgcgtgaa ttcgcgttac cttcatgaat tgcgatagat taacacggat
     1081 gaaacaccga tgagctgctg gttgtggaat ctttatctgt gtgaatctgt gttcatctgt
     1141 ggataggtag gatcgagatg aacgggcgcc tcgcgatcga tagcggagtc ctgcttctgt
     1201 atttcgtcat catcatcgcg atcgggctat tcatgggccg gcgcgagcgc gacttggagg
     1261 atttcgcgct tggtcgccgg caaatcccgt ggtgggccgt gctcgcttcc atcatcgccg
     1321 cagaaaccag cgccggaact tttttcggcg cgcccggcga aggtttcgcg ctgcgcaatt
     1381 acacttatat ccagctcgcc ttcggcacga tcatcgcgcg cgttctggcc agctacattt
     1441 tcatcaagcc ctactacgat tacaaagttt tctcgatcta cgaatacctg accgcgcgtt
     1501 tcggcgtcgg cacaaaaaac gccgcatccg cggttttcat gttcacgcgc gtgctcgcgt
     1561 ccggtgcgcg gctttacgtc gccgccatcg cacttgtgct cgcgtacgaa atgatccgcg
     1621 gcgcccggcc tggccaaacc gaaacgcttt tcatttatat cggcgcaaca atcgcgatcg
     1681 tggttctgac cgcgatctac acgacgatcg gcggaatcaa agcggtcgtc tggaccgatt
     1741 tcattcaagc ctccatcatg atgggcagcg ctctaaccgc gcttggcatt ttgtatttcg
     1801 cgatccccgg cggtctgcac gagatcgtgc aacgtcatgg cggttttcat ttgtctgatg
     1861 tcatcaccac cggtctcgat ccggcgaaaa gcggttggga caaaatcaaa ggcatgttcg
     1921 agatcgagta cacaattttt gccgggctca taggatcgac attcatcacc atggccacgc
     1981 acggcaccga tcaggacatg gtccaacgca tgttgaccgc gtcggacatt cggcggagcc
     2041 ggcgctcgct cattctttcc gggctggccg atattccgat cgcgctgacg tttttgacga
     2101 ttggactttt gctctgggtt tattaccaaa cgcatttcga tccaaatctt cccaaatcgc
     2161 cgaacgaaac tttctgccac tttattttgt acgaaatgcc ggtcggactc cgcggattgc
     2221 tcatcgccgg aatttttgcg accgccatgg gctcgctcag caccgcgatc aacgcgctcg
     2281 ccaccagttt cacgcgcgat tggtacgagc cttacataaa cccgcatgcg accggcgaac
     2341 aaagtctgcg cacggtccgt tgggccacag tcggcttctc gatcttaatg atcatcgtcg
     2401 cgtcgctcac atcctacttc gttatcgtga tgccgaacgt gcgcattatt ccgatcgtgc
     2461 tcggaattta tggttacact tacggctcgg tccttggaat ttttctcgcc ggcatgctca
     2521 cgaaaacgcg cggcaacagt cgtggcaaca tcatcgcaat gatcacgggc ttcatcgttg
     2581 tctcgatctt aagcggcctg cccaacaaca tcgccggcat tttcggcgca aaattttaca
     2641 cccaaccgac ctggctcccg ctcatggaat ttccctggtg gatctgtttc ggcacaatcg
     2701 tcactttttc ggtggcgatt cttttccgga cgggccatga acatcacctg ccaatttgat
     2761 ggtcgagcga acggcgtttc gtgaacctgc ccaatcccga tcagttaatc ggcgaccgcg
     2821 aaaaaattgt ggattagctc cttaatcccg ctcacccgta cggtacaagc aaggctcgat
     2881 tcttccgcgc gtgcggattt cgcagcgaag atcgggaaac actggccgaa gcgttgcgcc
     2941 agcacggacg cgaacaccaa gtgattcgaa cgaccgagac cggattcgga ccccgctatg
     3001 aagtcgaagg agaaattcaa gcgccggatg gaagccgacc gcgcgtcaaa accgtttagc
     3061 aattcgactg cggccaaatt gcaccacgtc tgattactgc ctatccttca aaacacatac
     3121 atgattaaag aacacgaccg cgttgtactg accgatgatg tgccaagcga aaggctgaag
     3181 gctggagaca ttgggactat cgtccacatt caccgtggcg gcgaagccta cgaggtcgag
     3241 ttcatttgct tagacggcga aacgattggc gtcgttacac tcaaggccag ccaggtgcgc
     3301 ccggcccaaa gtagggaaat cgcccacgcg cgcgagctcg cacgcaaccc ttagcaacgc
     3361 aaatctatgc cccacgtcgt gaccgacaaa tgcaacggat gcaagttcac cgactgtgtg
     3421 gaggtgtgtc ccgtcgcctg cttctacgaa ctggacagcc aggtggtcat tcacccggaa
     3481 gattgcattg attgcatggc atgtgtggac gtatgccccg tccacgccat ctacgcggaa
     3541 gacgacgttc cgccagaatt cgcgaaagat gtcgagttca acgcggttca gtccaggcag
     3601 ctgaaggact cgggagcgcc ggcaatcacc gcgcgcaagg agcccctgcc cggcgccttg
     3661 gagcgaaaaa cggccctggg ttattaagtg atcagcccat gctcgggatt ttgcaccggg
     3721 tgtaatccgt tctctctcat ttcttttccc gcgccgattg ccaccttttg cgtggcgatt
     3781 cttttctgga ctgaccacga gcatcagccg ccggtgtagg ggcatcgtgt cctcacgcgc
     3841 aatcgttcga agctgagcgt tggacgctga gcgccccgcc gcgtccgtag cgttgcgaag
     3901 gcgtgttgga cgtttagttt tgccgtcaat gcccgctcct cgcgtcatcg gtgaccttgt
     3961 tgagcgtttc gagcaacaag gcgacgccta caagtcaggc caatacaacg aagcccagct
     4021 gcgaaaggag tgcgtcgacc cgatgttcaa agcgctcggc tgggacatgg acaacatcag
     4081 cggttatgcc aaagcctaca aagatgtcat tcatgaagac gcgatcaaga tagggggcgc
     4141 aaccaaagcg cccgactacc caccgacaaa gctgacaaag atcgacacga tcgaatggtc
     4201 tcgctggtcg agc
//
LOCUS       DASTDG010000036         9065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1202717, whole genome shotgun sequence.
ACCESSION   DASTDG010000036 DASTDG010000000
VERSION     DASTDG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202717"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1278)
                     /locus_tag="VFO30_01200"
     CDS             complement(127..1278)
                     /locus_tag="VFO30_01200"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9417932.1"
                     /translation="MNLTLLERPLFYVSGHYVSFLGLIAFAALFGIGLTIARALQSNV
                     VRRFLSRFKLETKFIAIVTTILSLAALVFFTVSAINAAGLPLSWSQPLAGINLSLLQA
                     FLLVGLLIVVFWISSRTKRFLFTRFLANSGLDRSLQYAISQIIANIVLVIGVFIVLEN
                     TGIHLGALTVFAGAVGVGVGFGLQNIASNFISGLVILAERPITIGDRVEVAGIAGQVQ
                     QIRARSTVIMTNDNIAMIVPNTKFIDSPVTNWTYGDPRVRFRVPVGVAYGSDVNKVRD
                     ALIAAAREHPAALSDPPPNAFLDKFGASSIDFELVVWSREMSYRPRRFKSDLNYLIEK
                     HLRAAGIEIPNPQRDLHIRSGVLRVENVDSLAAASSSGGEQDRHIEGRA"
     gene            complement(1350..1652)
                     /locus_tag="VFO30_01205"
     CDS             complement(1350..1652)
                     /locus_tag="VFO30_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9417933.1"
                     /translation="MAEEKSIEEKVKDIIVEQLGVNPEQVTPQASFIEDLGADSLDIV
                     ELVMAFEEEFSVEVPDEDAEKLQTVGDVINYIKERAKQQGGGGSSSSDAGGGGGAA"
     gene            1832..3190
                     /locus_tag="VFO30_01210"
     CDS             1832..3190
                     /locus_tag="VFO30_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417934.1"
                     /translation="MPERSDLIDLVDLKMLPAWVKQPAATDYAFSGEEYDRPPRRERR
                     TRAPASRMRKAGPDGNDRRRRAQDRHRAAQEREGRLQPKPAPPQVAVGFLPEARALEI
                     VVMQISAGAVAYSLFSLARLFLQKPQRYQVHLAAKPASPLFQLGEGGPISVARECLES
                     NAFCLAQTEFYKIDITQTEPIKGNFTRVARDRLSGTLLGPTNHHGYQPRLRSLYEQRF
                     SRQMSFADYQRQIEIVSDPALVEHWKQEARIVTTYTTLREEPPLTFNSAIDAERHFRK
                     AYLPGLVRTIQETTIDGLTSRQMPDRTLRRLIENAWATQNRSPSQMMQELATGFRAAG
                     LHIFRHRRGMLFVSPVRARAFVHEETGVSPQVQAILQTLAEHPRMNRKDLADKLIVDL
                     ASAEAESHKLALAADLHWLIDEGYVIEFNDGSLDLPRMKKVAQASALQSEQTANEPLA
                     LP"
     gene            complement(3187..3660)
                     /gene="rpmB"
                     /locus_tag="VFO30_01215"
     CDS             complement(3187..3660)
                     /gene="rpmB"
                     /locus_tag="VFO30_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9417935.1"
                     /translation="MALPHNVAGADDPDAKFLMIFVHLPSVRLSILHARHRGRELHLT
                     SCAIVSFNNRLTVTRLHSTFRARFAIMAKVCCITGSRAARGSVIHRRGMAKKKGGVGR
                     HVTKNVPRVFAPNLHSHRIWVPELKRFVRLRVTARGLKTINKNGTYKALKKAGAI"
     gene            3592..4629
                     /locus_tag="VFO30_01220"
     CDS             3592..4629
                     /locus_tag="VFO30_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9417936.1"
                     /translation="MHKNHQKLRIGIVGAGNIVRQRHMPALKTRPEVEVVAVSNSTYE
                     SGEQFVKEFCPSATPMRNWADLLALPDLDIIWIGTPPYMHSAVTVSALEAGKHVFCQA
                     RMAMDLAEAEEMLAVSKRYPELVTMLCPPPFGMRGDLLMKKLLAESFIGTPHDVRLQS
                     FTSSYLNPEAPAHWRQKIQISGLNVLTLGIYVEVLQRWLGDIVGVFAHGKILYPDRRG
                     YNVIVPDLLNVLCKFENGAEGVLEFSGVNVSDETDRVEIYGDKGTLVYDFGTDKIRAG
                     KHGSREFNDVDLPPELTSEWRVEDDFIAAVKSKGRIRPHPDFEDGVRYMRVVQAVADS
                     RDRNEWVDVKK"
     gene            complement(4632..5669)
                     /locus_tag="VFO30_01225"
     CDS             complement(4632..5669)
                     /locus_tag="VFO30_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET9417937.1"
                     /translation="MLLFIAVVITALIFEYSNGFHDAANAIATVVSTKVLTPRQAIIM
                     AAVFNLTGALLGGAVATTVGKGLVDTNVVTLATVLCALIAAFAWNILTWWVGLPSSSS
                     HALIGGLCGAALATGHGSWSVLKWSTGLWPKVVLPMFTSPVAGFIVGALLMFLLFVTL
                     HRFTPYMVHSTFGKLQLVSAAWMAHSHGTNDAQKTMGIITLALFTGTKAGNFNDASPL
                     LHFLRTPTFSAIPVWVKIVCAITMAVGTAAGGWRIIRTLGHRVVKLQPVHGFAAETTA
                     ALIIQGASYYGIPLSTTHVISTSIMGVGAMKRFSGVKWLVVERIIWAWIFTLPATGLI
                     GYILARAVGAH"
     gene            complement(5703..6341)
                     /locus_tag="VFO30_01230"
     CDS             complement(5703..6341)
                     /locus_tag="VFO30_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET9417938.1"
                     /translation="MISIGRFLGHDEKFFDLLEASAQQADSSVHHLVELLHKIDNDDS
                     VRDLAAFAESRREDKRITQQLTEELSKTFITPLEREDIQALAAALYKIPKTVEKIGER
                     MLISPQDLPKVSFRKQVELLDRAAETVLAMVRQLRKGTDIRTAREMNAKLQAIEGDAD
                     HLELELLRDLYHGNYDPKHIIFLRDLYELLEKVIDRCRDAGNIVLQTVLKYS"
     gene            complement(6357..7718)
                     /gene="mgtE"
                     /locus_tag="VFO30_01235"
     CDS             complement(6357..7718)
                     /gene="mgtE"
                     /locus_tag="VFO30_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9417939.1"
                     /translation="MIAARNFTALRELFHEWPPADVAEVILDVPENDRVIIFRVLPHA
                     LAADVFEYLDVEAQQELLHAMAHEQVVGILNEMSPDDRTALLEELPSAAARQLIKLLT
                     PEERQIAQALLGYPEGSVGRLMTPDFIAVDEDWAVKEVLDFIRENGQDSETLNVIYVV
                     DDRGKLIDDLRIREFLLRPLDAKVSAFRDHTFVALRVNDTQEDALKAFRKYDRAALPV
                     IDSNGVLVGIVTSDDMLDVAEEEATEDIQKFGGMEALDEPYMRISLWRMVRKRAGWLV
                     ILFLGEMLTATAMANYQDEIAKAVVLALFLPLIISSGGNSGSQASTLIIRAMALGEVT
                     LRDWWRVASREIRAGLSLGGILGAIGAARVTIWSIVGERYMHRQLYGPHWALVAFTVG
                     AALVGVVLWGSLSGSMLPFLLRRLGADPATSSAPFVATLVDVTGLVIYFSIALVIMRG
                     AML"
     gene            7908..9065
                     /locus_tag="VFO30_01240"
                     /pseudo
     CDS             7908..9065
                     /locus_tag="VFO30_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
BASE COUNT         1983 a         2657 c         2643 g         1782 t
ORIGIN      
        1 agtaacgatg atgcttacat cggtcgcgga acgcctgttg ccattccggc agattcccaa
       61 ggtcgctgcg cgacacgact tcagcgacgc cattcggcgt ttccggggtc gtctccacgc
      121 cgttcgttag gcgcgcccct cgatatgtcg atcttgttcg ccgccggacg aactcgctgc
      181 ggcgagcgag tcgacatttt ccactcgcag cacgccgctg cgaatatgca ggtcgcgctg
      241 cggattcgga atctcaatgc ccgcggcgcg aagatgcttc tcgattaagt aattcaggtc
      301 actcttgaac cgccgtggcc ggtagctcat ctcccgactc cacacgacca gctcgaaatc
      361 gattgagctg gcgccgaatt tgtcgagaaa cgcattcggc ggcggatcgc ttagcgccgc
      421 gggatgttcg cgcgccgcgg cgatgagtgc gtcgcgcact ttgtttacat cgctgccata
      481 agcgacgccg accggcacgc gaaaacgcac gcgcggatcg ccataggtcc agttcgttac
      541 cggtgagtcg atgaacttcg tgtttggcac gatcatggcg atgttgtcgt tggtcatgat
      601 gaccgtgcta cgggcgcgga tttgctggac ctggccggcg atgcctgcga cttccacacg
      661 atcgccgatc gtaatcgggc gctccgcgag aatgaccagg ccactgatga aattgctcgc
      721 gatgttttgc aggccgaagc cgacacccac gccgacagcg cctgcgaaga cagtgagcgc
      781 gccgagatgg atcccggtgt tctcaagcac gatgaaaaca ccgatcacca gaacgatgtt
      841 cgcgatgatc tgcgagatgg cgtattgcag ggaacgatcg agtccgctgt tggcgaggaa
      901 acgggtgaag agaaaacgtt tcgtgcgcga cgaaatccag aagacgacaa tcagcaggcc
      961 aacgagcagg aatgcctgga gcaaactcaa gttgatgccg gcgagcggtt gactccaaga
     1021 gagcggcaga ccagcggcgt taatcgcgct tacggtaaag aagacgagcg cggccaggct
     1081 caagatcgtc gtgacgatcg cgatgaactt tgtctcaagc ttgaaccggc taagaaaccg
     1141 ccgcactaca ttgctctgca atgcgcgggc gatagttaat ccgataccga acagcgccgc
     1201 aaaagcgatc agccccagaa aggagacgta atggccactg acatagaaca acggccgctc
     1261 cagcagcgtc aggttcacct tagctagtct gggcgcgttg cgtcagttcg caaccaccaa
     1321 cgctggacgc ttgcgcgccg cctgcgggct caggcagcgc cgccgccccc gcccgcgtcg
     1381 ctgctgctac tgccgccgcc gccctgctgt ttggcgcgtt ctttaatgta attgatgacg
     1441 tcgccgacag tctgcagttt ctcggcgtct tcatccggca cctctacaga aaactcttct
     1501 tcgaacgcca tcactagctc cacgatgtcg agcgaatctg cgcccaaatc ttcaataaac
     1561 gaggcctgcg gggtgacctg ctccggatta actccaagct gctcgacgat gatgtccttg
     1621 accttctctt caattgattt ttcctcggcc ataaaaaatt cggtaggttg gagggcaagg
     1681 cttaccccac gctccaaagc agcgcaagca aaacaattgt tcaccgcgcc gtagtcttcg
     1741 acggaggcgg gtcgatcttt ccgcgcaatt aaaaacattt cgcctgccgt ttcgcgggcg
     1801 tatgatcgat cgcggctgaa ggagaacaat cgtgcctgaa cgcagcgatt tgatcgatct
     1861 ggtcgacctg aaaatgctac ccgcctgggt gaaacaaccc gcggcgacag attacgcatt
     1921 cagcggcgaa gaatatgacc gcccgccgcg tagggagcga cgaactcgcg cgccggcaag
     1981 ccggatgcgc aaagcaggac cggacggcaa cgatcgcagg cgacgcgcgc aagatcggca
     2041 tcgtgcagcc caagaacgcg aggggcgttt acaaccgaaa ccggcacctc cgcaagtcgc
     2101 ggtgggtttc ttgccggaag ctcgcgcgct cgaaattgtc gtcatgcaaa ttagcgcggg
     2161 tgctgtcgcg tactcgttat tttcgctcgc tcgcttgttt ctgcaaaaac cgcagcgcta
     2221 tcaggttcat cttgccgcca aacctgcgtc gccgctcttt cagcttggcg agggcgggcc
     2281 gatttcagtc gctcgcgagt gtctcgaaag caatgcgttt tgcctggcac aaacggagtt
     2341 ttacaagatc gacattacgc aaaccgagcc gatcaaggga aacttcaccc gtgtcgcgcg
     2401 cgatcgttta agcggaacat tgctggggcc aaccaaccat catggttatc agccgcgctt
     2461 acgcagcttg tacgaacagc gcttcagccg gcaaatgagc ttcgcggatt accagcgtca
     2521 gatcgaaatc gtgagcgatc ccgcgctggt tgagcactgg aaacaggaag cacgtatcgt
     2581 taccacctac acgacactgc gcgaagaacc gccgcttacg ttcaacagcg caatcgacgc
     2641 ggagcggcac tttcgcaaag cctatttgcc cggcctcgta cggacaattc aagagacgac
     2701 gattgatggc ctaacgagcc gacagatgcc agaccggaca ctgcggcggc tcatcgagaa
     2761 cgcctgggcg acacaaaacc gttcgccgtc gcagatgatg caggaactcg cgactggttt
     2821 tcgcgcagct ggtttgcaca tttttcgcca tcgccgcggc atgttgttcg tgtcgcccgt
     2881 ccgcgcccgc gcattcgtgc atgaggaaac cggggtgtcg ccgcaggtgc aggcaattct
     2941 gcaaacactc gccgaacacc cgcggatgaa tcgcaaagat ctcgcggaca aactcattgt
     3001 cgatcttgcc agcgcagagg cagagtcgca caagctggcg ctcgcggctg atctgcactg
     3061 gttgatcgat gaaggctacg tcatcgagtt caatgacggc tcgctcgatc tgccgcggat
     3121 gaaaaaagta gcgcaagcct cggccttgca gtcggaacaa acggccaacg agccgcttgc
     3181 cctaccttaa atcgcgccgg cttttttcag tgccttgtag gtgccgtttt tgttgatcgt
     3241 cttcaggccg cgtgcggtca cgcgcagacg aacaaatctt ttcagctccg gcacccagat
     3301 gcggtgcgag tgcaggttgg gagcaaagac gcgcggcaca tttttcgtca cgtgccgccc
     3361 gacgccgcct ttctttttcg ccataccgcg gcggtgaatg acgctgccgc gcgcggctct
     3421 ggatccggtg atgcagcaaa ctttcgccat aattgcaaaa cgcgcgcgaa atgtgctgtg
     3481 cagtcgagta acggtcaagc gattgttgaa cgaaacgatg gcacaactag tcaaatgtaa
     3541 ctcgcgtccg cgatgacgcg cgtgcaagat cgacaatcgt actgagggca gatgcacaaa
     3601 aatcatcaga aacttcgcat cgggatcgtc ggcgccggca acattgtgcg gcagcgccat
     3661 atgccggcgc tgaaaacgcg gcccgaggtc gaagtggtgg cggtgtccaa ttccacttac
     3721 gaaagcggcg agcagtttgt taaggaattt tgcccgtcgg ccacgcccat gcgcaactgg
     3781 gccgacctcc tcgcgctacc ggacctcgac atcatctgga ttgggacgcc cccctacatg
     3841 cattcggccg tcacggtctc ggcacttgag gcggggaaac acgtcttttg tcaggcgcgg
     3901 atggcaatgg atttggcgga agctgaggag atgctcgcgg tctccaagcg ttaccccgag
     3961 ttggtgacta tgctgtgtcc accaccgttt ggaatgcgcg gtgatctgct catgaaaaag
     4021 ttgctggctg aaagtttcat cggcacgccg cacgatgtgc ggctacaaag tttcacgagt
     4081 agttatctca accccgaagc gcccgcgcac tggcggcaga aaattcagat cagtgggttg
     4141 aacgtgctta cgctcggcat ttacgtcgaa gtgttgcagc gctggctggg tgacatcgtc
     4201 ggcgttttcg cccacggcaa gattctgtac cccgaccggc gcggctacaa tgttatcgta
     4261 ccggatttgt tgaatgtgct gtgcaagttc gagaatggcg ccgagggcgt actcgaattc
     4321 agcggtgtca atgtctccga cgagaccgat cgcgtcgaaa tctacggcga caaaggcacg
     4381 ctggtttacg atttcggtac ggacaaaatt cgcgcgggta aacatggcag ccgcgaattc
     4441 aacgacgtcg accttccgcc ggaactaacg agcgagtggc gcgtcgaaga tgattttatc
     4501 gccgcggtga aatcgaaagg ccggatccgt ccgcatcccg acttcgaaga tggcgtgcgc
     4561 tacatgcgcg tcgtccaagc cgtcgccgac tcccgcgacc gcaacgagtg ggtcgacgtg
     4621 aagaagtaaa atcaatgcgc gccgaccgcg cgggcgagaa tataaccgat aagaccggtg
     4681 gcgggcaggg tgaaaatcca tgcccagatg atgcgctcga cgaccagcca tttaacgccg
     4741 ctgaagcgtt tcatcgcgcc tacacccatg atagaagtcg agatgacatg ggtcgttgag
     4801 agcggaatgc cgtagtagct cgcgccctga ataatcagcg cagcggtcgt ttccgcggcg
     4861 aatccgtgca cgggctgcaa cttcacgacg cgatggccaa gggtgcggat gatccgccac
     4921 ccgcccgctg ccgtgcctac ggccattgtg atagcgcaga caatttttac ccacaccgga
     4981 atcgcgctga acgtcggcgt gcgcagaaaa tgcaacagcg gagacgcatc gttgaagttg
     5041 ccggcttttg ttccggtgaa gagcgcgagc gtgatgatcc ccatcgtttt ctgtgcgtca
     5101 ttggtgccgt ggctgtgcgc catccatgcg gcgctcacca gctgcaattt tccgaacgtt
     5161 gaatgtacca tgtagggagt gaaacggtgc agcgtgacga acagcaaaaa catcaacagt
     5221 gcgccgacga tgaatccggc aacgggcgag gtgaacatcg gtaaaacaac tttcggccac
     5281 agcccggtgc tccatttcag caccgaccaa ctgccgtgac cggtcgcgag cgcagcgccg
     5341 cacaatccac cgatcagcgc gtggctggag ctcgacggca ggccgaccca ccacgtcaga
     5401 atgttccagg caaacgccgc gattagcgcg cagagcacgg ttgcaagggt gaccacgttg
     5461 gtatcgacca agcctttgcc gacagtggtg gcgaccgcgc cgccgagtaa cgcgccggtc
     5521 aaattaaaaa ctgcggccat gatgatcgcc tggcgcggcg tcagcacttt ggtcgacacg
     5581 accgtcgcga tcgcattcgc ggcatcgtgg aaaccgttgc tgtattcgaa aatgagtgcg
     5641 gtgataacaa ccgctatgaa aagcaacatg tcgatgttgg tccgccgggg cggggcggcg
     5701 aattacgaat atttcaggac agtttgcagg acgatgttgc ccgcgtcccg gcagcggtcg
     5761 atcaccttct cgagcagctc gtaaaggtcg cgcaagaaaa tgatgtgctt cgggtcataa
     5821 ttgccgtggt aaagatcgcg taaaagctcg agctcgagat ggtcggcgtc gccttcgatg
     5881 gcttggagct tggcattcat ctcgcgagcg gtgcggatgt cggtgccttt gcgcaattgg
     5941 cgcaccattg ccagtaccgt ctccgccgca cggtcgagca gctcgacttg tttccgaaaa
     6001 ctgaccttcg gcaaatcctg tgggctgatc agcatccgct cgccgatttt ctcgaccgtt
     6061 ttgggaattt tatagagcgc ggcggcgagc gcctggatgt cttctcgctc cagcggcgta
     6121 ataaacgtct tcgacagctc ctccgtgagt tgctgagtga tgcgtttgtc ttcgcggcgg
     6181 ctttcggcaa atgccgcgag atcgcgaacg gaatcgtcat tgtcgatctt gtgcaaaagc
     6241 tcgacgagat ggtggacact cgagtccgcc tgttgcgcgc ttgcttccag aagatcgaaa
     6301 aatttttcgt cgtgtccgag gaaccgtccg attgaaatca taaaacgcct ccgcgtctaa
     6361 agcattgccc cgcgcataat gaccaatgca atactgaagt atataacgag cccggtcacg
     6421 tccacgaggg tggccacgaa aggcgcggaa gaggtggccg ggtccgcgcc cagccggcgg
     6481 agaagaaacg gcagcatcga cccggaaagt gagccccaca ggacaacacc gaccagcgcc
     6541 gcgcccacag tgaacgccac cagcgcccag tgtggtccgt acaattgccg atgcatgtag
     6601 cgctcgccaa caatcgacca aatcgttacg cgcgcggcgc caattgcgcc gagaatcccg
     6661 cctaacgaga gacccgcgcg aatttcccgg ctcgccacac gccaccaatc gcgcagcgtc
     6721 acttcgccca gtgccatcgc acgaatgatc aacgtggatg cttgcgagcc ggaattgccg
     6781 ccactggaaa tgatgagcgg caaaaacaac gccagcacta ccgccttggc gatttcatcc
     6841 tggtagttgg ccatcgccgt tgccgtcaac atttcgccga gaaaaagaat caccagccag
     6901 ccggcccgtt tgcgtaccat gcgccacagc gagatgcgca tgtaaggctc atccaacgcc
     6961 tccatgccgc cgaatttctg gatgtcttct gtcgcttctt cttccgcaac atcgagcatg
     7021 tcatcgctcg tgacgatgcc gacgagcacg ccattggagt cgatcaccgg caaggcggcg
     7081 cggtcatatt tacggaacgc tttgagcgcg tcctcctgcg tgtcattcac tcgcagcgcg
     7141 acaaaagtgt ggtcgcggaa cgcgcttact ttggcgtcga gcggccgcag caaaaattcg
     7201 cggatccgca ggtcatcaat caattttccg cgatcatcga cgacgtagat gacgttaagt
     7261 gtctcgctat cctggccatt ctcgcgaatg aaatcgagga cttctttcac cgcccagtcc
     7321 tcgtccacgg ctatgaaatc gggggtcatt aagcggccga cactgccctc ggggtaaccc
     7381 agcagcgcct gtgcgatctg ccgctcctcg ggcgtgagca gcttgatcaa ctgccgcgcc
     7441 gccgcgctcg gcaattcttc cagcagcgcg gtgcgatcgt ccggcgacat ctcgttcaaa
     7501 atcccgacca cctgttcgtg cgccatggcg tgcaaaagtt cctgctgcgc ctcaacatcg
     7561 agatactcga aaacgtcggc ggcgagcgcg tgcggcagca cccggaaaat gataacacgg
     7621 tcgttttccg gaacatcgag aatcacttcg gccacgtcag ccggcggcca ctcgtgaaac
     7681 aactcgcgca gggcggtgaa attccgcgca gcgatcatgc tctggatttc gggagcgaga
     7741 agtttaccga ccatagatat gccgtcgcaa ttgtagcggc gcttgtacca agcaccgaac
     7801 ggaaatacaa gcgcgccgcg gcgagcgcct cgacaattgt ctccgcgctt ggcaatcaat
     7861 cgaaacgcgc caatatgaat ccgatgagcg caaccgcgac cgcggcgatg atccagcgca
     7921 agatcgacaa tgcaatttgc gtgctcacct tcgatcggcc cgaatccggt gcgaatgtct
     7981 tcgacgcggc cacaattaaa gaattcgacg cgcatctcga cgcgattgaa agcgactcgt
     8041 ccctgcgcgg cctgatcatt acttcagcca aggagtcgat cttcatcgcc ggtgccgatc
     8101 tcaggacact tttcagccgg gcgaaaactg gcgagctgca cgggtttatt gccgacgggc
     8161 aacgcgtttt cagtcggatc gcggcgctga aaatcccgac tgtcgccgcg attcacggcg
     8221 cgtgcgcggg cggcggatac gagattacgc tcgcgtgcga ttaccggctc gcgtcggacg
     8281 atccggcgac gcggatcggt ttacccgaga cgacgctcgg cctggttcca gcctggggcg
     8341 ggtgcacccg tctgccgcga ttgattgggc tcgacgccgc gtgcgaagtg attctcaacg
     8401 gcaaactcta tgccgcgctt gaggcgctga agctcggcct agtggacgag gtggtttcgc
     8461 gtgagcactt ggtcgatgcg gcccgcaata aaattgcgac gggcaaaaga tcgatgcctg
     8521 cgccgccaca cattgaaaaa caaccgaaac cgccgccggc caatggtaat gccgcgccac
     8581 ggcgcgcgtt cgagatcatt cgccaggcga tcggcagctc agtcgatgag tcactgcggc
     8641 gcgaactcga ggcaattgtc gatcttggca agacggaagg aacgcagaat ctgatccgca
     8701 atttttttct ggcggagaaa tacaaaaagg gcgacgggaa attctcgcaa gccaaggtcg
     8761 tgcacgcggc cgtgatcggc gcgggtgtga tggggtctgg catcgcgcaa tggctgagcg
     8821 cgcgcggcgt aacggtgatt ttgcgcgacg tcagccgtga gttactcgat cgcggtttgg
     8881 caaacatcga gaagatttac agcgacgcgg taaagcgtgg cttgatgacg gaagacaaat
     8941 cgaagggggg ccgcgcgcgg attgtcgctt caaccgcgcc tatgtaattg ctcgatgtgc
     9001 agttcgtaat cgaagccgcg tcggaaaaga tcgacgtcaa aaaacatatc tttcgcgagc
     9061 tctcg
//
LOCUS       DASTDG010000037        13766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_397974, whole genome shotgun sequence.
ACCESSION   DASTDG010000037 DASTDG010000000
VERSION     DASTDG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397974"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFO30_01245"
     CDS             <1..275
                     /locus_tag="VFO30_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9417940.1"
                     /translation="KHLPSQLSGGQQQRVAVARALAGRPAILLADEPTGNLDSRNGEA
                     VMQLLKELHGGGATICMVTHDERFAQHADRTVHLFDGRVVEDRIAA"
     gene            287..2764
                     /locus_tag="VFO30_01250"
     CDS             287..2764
                     /locus_tag="VFO30_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417941.1"
                     /translation="MQTLWQDLRYALRMLAKQPAFTATAILTLALGIGANTAIFSVIN
                     AVLLRPLPYPQPDRLTLIRERTNIFDSGSVSLPNYLDWRVSQRGFTDLALFRRDDANL
                     SGAASDVEAERVGSLRVTYNFLSVLAVPPELGRDFRESDDVPHCKKVALISEELWKRR
                     FGASHDVIGQSVMLDGVQREIIGVVPSNVQLARKAQVYIPLEDLRADKDYLDRRNHPG
                     FSALGRLKPNVALAQGTADLNDIAAELERRYPDSNTGRRVTALILLDSAVKDYKHGVT
                     LLLAAVGCVLLIACANVANMQLARALGRERELAVRAALGASRSQLAKQVFIESAILAV
                     LGAIAGVLLALWGLDAIKAIAPGHSASFAPSDATRFQEANLDLKVLAFTAAVAVAAGV
                     LVGIWPALRVSRTASLTLSLHEGGRGTSGGAHSQRVRSGLVVAQVALALLLLLGAGLT
                     LKSFRNAQNARLGFNPENILVADVSLPKARYDTDEKIARFNDQLIDRIRLLPGVQAAA
                     LGSNIPFDNNEWDSSFHLTGTPPYPPGERPEAEVNHVTPDYFRVMGMPLLRGRAFTAD
                     DRAGRPRLVIIDETLAQKYFPGKDPIGQQMDDNRSDEKNPPPLTIVGVVPRTRNEAPG
                     EDNVEQYHWPQMTFAADQVPNRSNMLLVRVKSGNPLALVPAIKRELQGLDPDQAFAGI
                     STMESNIEKSLGSRRMMMSLLSEFAIIALVLASVGLYGVMALTVTQRTRELGIRLALG
                     AQRADVFRLVLAQGMLLVLGGLTIGLLGAVGAARGLQSVLYGVGRFDVSALSVALLAL
                     AVVALLACWLPARRATRVDPIEALRAE"
     gene            2766..5186
                     /locus_tag="VFO30_01255"
     CDS             2766..5186
                     /locus_tag="VFO30_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417942.1"
                     /translation="MNDLRFALRQLRKSPGFTFLAVLTLALGIGMNTAIFSLIHDLFL
                     RRLPFQEPEQVVRIYGEAKDRDLKELPFSVPKFWHYRDGQTAFSSIAADRGNGFILTE
                     IGEPIQLFGRNVTANYFDLLGIHPTLGRTFLPEEETKGDVVMVTESFWRKRLNSDPNV
                     LGRSITLNGVPNTIIGVLPTLPISWFGRDTEIFANKLFEPADISRDRLMRGVSFMRAI
                     ARLKPGETIQQAQATMPALERGYREERPENPDNSWASVLVPAAEDVTGNLRPAFMTLL
                     AAVSAVLLIACSNVANLLLVRFTGRRREIALRMAVGGQRSSIVRLFVLESTLVSVIAG
                     VAGLCLALWTVSVVPRMAGQNVPLESGVSLHLPVLAFTLGLSLITGLLMGLYPAWQSS
                     RADLVEGLKEGGGGTTGSHRQHRLRRGLVAAQVGLSVALVASAAMLISSFVRLSRQES
                     GFRSERIWAGGIALPPARYPDPASQARFVQRLTTELQNEPGVEIASVTGAVPLSGDIS
                     RAPYARVDGNPLPLTQRPLGLTRSVSANYFRTLSIPLVSGRDFSERDGVDQPLVVILS
                     KATAKKLFPNEDPLGHRILFGTDNGTGAPAEVVGIVGDVRSAELAKANDVEFYRPWAQ
                     RSSPFLNVLVRSTTKPEATTGIVRLSLKKIDDGLPIIQPNTLEAIVSDSLGDRRLTMA
                     LLGVFAGIALLLAVVGIYGAVAYTVEQRTGEIGVRMALGAQTIDVLRLVVRQGMNPVI
                     LGLIIGLAGTFAVGRLLAAQLYQISPHDPFLLGAAATVLAVAALLACLLPARRATRID
                     PIQALRSE"
     gene            5188..7638
                     /locus_tag="VFO30_01260"
     CDS             5188..7638
                     /locus_tag="VFO30_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HET9417943.1"
                     /translation="MDDLRFALRQLRKSPGFTAVVVITLALGIGANAAIFTLVRGVLI
                     KPLVNRDEDRLIYIRQSATGIGTENSTWSMPEIADLKTRVETLSEFGDFSTIAFTMIG
                     LGEPRELNGGVVNGSYFDVMGLRPALGRLIGPEDDGPKAAPVVVLTYRFWSNALKRDP
                     SVIGKTVNLGGVEGDRSATIIGVLEPCVPYPQETEIIANVVSSPHHLSATMVTQRVHR
                     MTELFAHLAPGATLDQARVELRSVYATMKKDHPEAYSKQADFQISAKLLRDQITSGAR
                     TVLLVLLAASALLFVVACSNVANLILARTVRREGELAVRTALGASKGALRRMLLAESL
                     LLCGGGAALGVLSAQWMVDVLARYASRFSVRALDLTVDSSLLWVGVSLAIVAAVILAF
                     VPRLPADTSGALHLSSSSAALSGSKRRQRIFVVTQIGASFLLLAGAGMVVTTLLALQR
                     TQTGIDTRRVLAINVPAVFYGKTGRQVIDFYKEVIRRIDALPGVNRTAFGMIAPWRDA
                     GSGPTLQFNAEGYVNAAGEDDPRAQYRVISPGFFAALGVPIIEGRDFNDLDGRSEEPV
                     AIISQTLAQRMFPNQDPINRHVFWTDPVLKRIGKTPPRLQRIIGVAADIDDVHVVPEP
                     TLTIYHSFEQGPLFRGRLFIHASANPYSLITPVTRIIRDLSVDQPIERAATLEDARAE
                     VLTPHRLNSVVFGLFAAVALSIAVVGVAGVLAFSVSARTREFGIRLALGSQPKRLFKD
                     VIVEGAAMAAAGIMAGVIFGFLLAQVARSYFADATMPGVLPVAVSAFVLLAVALAASM
                     LPAHRAARVDVIQALRSE"
     gene            7640..10105
                     /locus_tag="VFO30_01265"
     CDS             7640..10105
                     /locus_tag="VFO30_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417944.1"
                     /translation="MNDLKFALRQLSKSPAFTLVAVLTLALGIGANTAIFSVVEGVLL
                     RPLPFPRAEQLVRIYEALDENGARTASLNLSDRTVARFREFGREIFEDVAGGTGGAAV
                     VGVNGASPVQTVPAAQVTSNFFDVLGLPPAQGRNFTAEEGLNGTASVAIVSDDFWRNT
                     LNSRRDVLGSSIVIDGTPRTIIGVMPKAFRHPYRANLWLPLALQPDNPATINNHYLYG
                     VARLRPGVTPQKAEDAVKRMCATINRDDPNPANARAAYIVPLRESFVMDLRPKILVIV
                     GAAVCALLIAAANFAGLLLARVVEREGEFALRAALGASRLRLVRQQLTQALSLASIGT
                     AFGLLIAFWITPMLFALSPEGTDATGSAMREFDYAARLDLPVFAFAAGVMTLVGLGFG
                     LLPAARASRTDLRCAMSITSRGATLDCNTRRLLGSFVVIELAIAAALLTASITATQYF
                     WKLIEEPWGFETKDRLAFNVTVPDRFFPSAAAKQNALEASLEQLRALPGVESATVVSP
                     SPMDASWTLMLFNVEGAPAPEPRGVYTAYSRVPVPGYFQSVGQPILQGRDFNTSDTAD
                     APLVCIISQSIAKRFWPNDSPIGKRIRWGRLDGTRPWFTIVGVVGDMKAIADPRDGEV
                     LGMIARPLAQMLVHATTQLDDITFVLHTDRRSVNEPVIRAALARANGNLAAYNFLALE
                     DAAARSRTTERFIFVLVSSFGFVGLMLAGVGLYGLLALQVARREREFGIRSALGATAR
                     QIIQLVARQGATLLSLGFTAGALATYGVVHLVRNQWTEMPAPNLVACICAAIVLGIAV
                     MIASWLPARRAARVDPVIALRAE"
     gene            10107..12521
                     /locus_tag="VFO30_01270"
     CDS             10107..12521
                     /locus_tag="VFO30_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417945.1"
                     /translation="MNDLKFALRQLRKSPAFTAIAIITLALGIGLNTAIFSLINDLFL
                     RGLPFRQPSRVLHVFSHARDQRVDFPLSAPRFMHFRDGQTIFDGFAGENGLAATVTGV
                     GDPFQVPIFKTTANYFDVLGVRVIRGRTFSAQEEEGADVAVVTERFWKSRLGGDPNVI
                     GRGLTLDGVSHTIVGVIPTLPVSWTGPNADIWTTKPFIIPGFSYERMMRGTAFLRAVG
                     RLKRGISVMQARAALQLLENSYRTQYPEKIDAKSSTIVKTLPEDVTENLRPGFATLLA
                     AVTFVLLIACSNVANLLLVRFTGRRREISLRMALGATRASVVRLFVFESVLISLLAGI
                     FGAVLAWQFVPVVPRLAANFLPIEQGSATAISFPVLGFTIALSLLTGLVMGLYPALQS
                     SRAEIVDALKEGGRGTSGSVHQQRFRKLLVGAQVALSVTLLAGASLLIASFVRLSRQD
                     PGFRFENLWTCFTVLPEAQYSDEGTRARFADQLTKTLRAMPGLQEATISTGIPLTGGA
                     AATLYARADSNVPPVAERKGAASNDISPGWLRTLGIPLLAGRDFTEQDVADHPNVILI
                     SASGAKTVFGNENPIGKTLLITSGSIPVEIVGVVGDVRSVRLDQRNDMEFYRPYAQEN
                     FPFLSITVRSSLPTDTVTKTVQTALRTIDPNLALILPQPVKEIMRQALGQAKLMMALL
                     GIFAGVALLLATIGIYGAVAYTVEQRTGEIGVRMALGAQTADVLRLVVRQGMAPVVLG
                     LVIGMVAALALGRLLSTQLYQVSPHSPALLATTALTLGAVALLACFLPARRATFVNPI
                     EALRAE"
     gene            12530..>13766
                     /locus_tag="VFO30_01275"
     CDS             12530..>13766
                     /locus_tag="VFO30_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417946.1"
                     /translation="MQTFFQDLRFGFRMLRRSPGFSLLAILCLTLGIGTNAAVLSWIE
                     GILIRPYPLVAHQDRMFALIGTTRGVPGHNGLSYPDFVDLEKNSTLFESFIIDRITGT
                     TLSVGDRAERASLGIVSANYFDALGVRPILGRGFRPEEATGRNAHPVTVIAYRTWKDR
                     YNFDPNIVGRAQFMNGVQHTIVGVAPEKFHGTFVGYSFSFWVPTSMQEAFDTTGYKLE
                     DRGARWIEGYAILKPGVSRQQAQAQLNSISQRLEKDFPDTNRGHENQLVPLWKTPFNG
                     AGNMSSTLAITMAVVFLVLLIACANVSNLLLARSLLRRHEMTMRLALGAGRGRLVRQL
                     VTEGLLLSLIAALGGIAVAYWCRNALVLAFPPALPGMVIDYPGQIDWRVLAVSAGICM
                     ASTLLFALVPAIRASDVDLS"
BASE COUNT         2562 a         4021 c         4059 g         3124 t
ORIGIN      
        1 cgaagcattt accaagtcag ctctccggcg gccaacaaca acgggtggct gtcgctcggg
       61 ctctcgcggg caggccggcc attctgctgg cagacgaacc gacgggcaat cttgattcgc
      121 gcaatggcga agcggtgatg caactcctca aggaactcca cgggggcggg gccaccattt
      181 gcatggtcac acatgatgag cgcttcgctc aacatgccga tcgcaccgtg catctgtttg
      241 acggtcgggt agtggaggac cggatcgccg cctagggagt tacgcaatgc aaacgctgtg
      301 gcaggacctg cgctacgcgt tgcgaatgct ggcgaaacag ccggcgttta ctgccacggc
      361 gatattgact ctcgcgctgg gcatcggcgc caatactgca attttcagcg tcatcaacgc
      421 cgtgctcctg cggccgttgc cttatccgca accggaccgt ctcacgctca ttcgcgagcg
      481 cacaaatatc ttcgattccg gttcggttag cctgccgaat tatcttgatt ggcgcgtcag
      541 ccagcgcggt ttcactgatc tcgcgttgtt tcgccgggac gatgcgaatc tgtccggcgc
      601 cgccagcgac gtggaagcgg agcgcgtcgg ctccctgcgc gtcacctaca actttctttc
      661 cgtcctcgcc gtgccgccgg aactgggacg cgatttccgt gagtcggacg atgtcccgca
      721 ctgcaaaaaa gtcgcgctga tttccgagga actatggaaa cgccgcttcg gcgcttcgca
      781 tgacgtgatc gggcaatccg tcatgctcga tggtgtgcag cgcgaaatca tcggcgtggt
      841 tccatcgaac gttcagttgg cgagaaaagc gcaggtttac attccgctgg aagatttgcg
      901 ggcggacaag gattacctgg atcgtcgtaa tcatcctgga ttctccgcgc tcggccggct
      961 caaaccgaat gtggcactcg cccagggcac ggctgattta aatgacatcg ccgcggagct
     1021 cgaacgtcgt tatcccgatt ccaacaccgg acgccgcgtc acggcactga tccttctcga
     1081 ttctgcggtg aaggattaca aacatggcgt gacactcctt ctggcagcag tcggctgcgt
     1141 gcttctcatc gcctgcgcga acgtcgcgaa catgcagttg gcgcgcgcgc tcggccgcga
     1201 acgcgaactg gctgtgcgcg ccgctctcgg cgcaagccgc agccaactcg ccaagcaggt
     1261 gtttatcgaa agcgcgatcc tggctgtgtt aggagctatc gcaggcgtcc ttctcgcact
     1321 ttgggggctc gacgctatca aggcaattgc gccgggtcat tccgccagtt ttgctccgtc
     1381 tgatgcaact cgtttccagg aagcgaatct cgatttgaaa gtgcttgcgt tcacggcggc
     1441 ggtggcggtc gccgccggcg tactggttgg tatttggccg gcactgcgag tctcccgcac
     1501 cgcttcgctc acgctctccc tgcacgaagg cggccgcgga accagcggtg gcgcgcacag
     1561 tcagcgtgtg cgatcgggac tggtcgtggc gcaggtcgct ctcgctttgc tcctgctcct
     1621 cggcgcggga ctaactctga aaagttttcg caatgcgcaa aacgcgcggc tcggattcaa
     1681 tccggaaaat attttggtgg ccgacgtttc gttgcccaag gcgcgctacg acacggacga
     1741 aaagatcgcg cgttttaatg atcagcttat cgatcggatt cgcttgcttc ccggcgtcca
     1801 agccgcggcg cttggctcga acattccctt tgataacaac gagtgggaca gttcctttca
     1861 tctgacgggc acgccgccat atccgcccgg cgaaagaccg gaggcagaag taaaccacgt
     1921 aacgcccgat tattttcgtg tgatgggaat gccgcttttg cgcggacgcg ccttcaccgc
     1981 agatgatcgc gccggccgcc cgcgattggt cattatcgat gaaacgctcg cgcaaaaata
     2041 ttttcccggc aaagacccca ttggccagca gatggatgac aaccgatcgg acgaaaaaaa
     2101 tccgcctccc ctgacgattg tgggggtagt cccgcgcacg cgcaacgaag cgcccggcga
     2161 agacaacgtc gagcaatatc actggccgca aatgaccttc gccgccgacc aggtgccaaa
     2221 ccgcagcaac atgctcttgg tgcgggtgaa atcgggtaat ccgctcgcgc tcgtgccagc
     2281 aatcaaacgc gaactccagg ggctcgatcc cgatcaagcc tttgccggca tctcgacgat
     2341 ggaaagcaac atcgagaaaa gtcttggctc ccgtcgcatg atgatgtcgc tactcagcga
     2401 gttcgccatt atcgcgctgg tgctggccag tgtcggattg tatggggtga tggcgctgac
     2461 agtgacccag cgaacacgcg aactgggtat tcgtctcgcc ctgggcgcgc aacgcgcaga
     2521 tgtcttccgc ctggtcctcg cacagggaat gttgcttgtg cttggcggcc tgaccatcgg
     2581 attgcttggc gcggtcggcg cagctcgcgg attgcaaagt gtgctgtacg gcgtgggcag
     2641 gttcgacgtg tcggcattga gcgtcgctct tttagcgctc gccgtggtgg cactgctcgc
     2701 ctgctggttg ccggctaggc gcgccacgcg cgttgatcca atcgaagcat tgcgcgctga
     2761 ataacatgaa cgatctaaga tttgctcttc gacagctccg caagtcgccc ggatttactt
     2821 ttctcgcggt gctcacgctg gcactcggca tcggcatgaa cactgcgatc tttagtttaa
     2881 tacacgatct tttcctgcgt cgcctgccgt ttcaagagcc ggaacaggtg gttcggattt
     2941 atggtgaagc aaaagaccgc gatttaaagg agctgccctt ttccgtcccg aagttctggc
     3001 attatcgcga cggacaaact gctttttcca gcatcgccgc cgatcggggg aacggtttta
     3061 tcctgaccga aattggcgag cccatccaat tgttcggccg caacgtgacg gccaactact
     3121 tcgatctgct cggcattcac ccaacactcg ggcgtacttt tctgccagag gaagagacga
     3181 aaggggatgt tgtaatggtg acggaaagtt tctggcgtaa acgcctgaac tccgatccta
     3241 atgtgctagg tcgcagcatc acgctgaacg gtgtacccaa tacaataata ggtgtgttgc
     3301 ccactctgcc gatttcatgg ttcggccgcg acaccgaaat ctttgcgaac aaactgttcg
     3361 aaccggcgga catcagtagg gaccgactga tgcggggagt cagtttcatg cgtgccattg
     3421 cgcgattgaa accgggcgaa acgatccaac aggcgcaggc caccatgccg gcactcgagc
     3481 gaggctatcg cgaggagcga ccagaaaatc cagacaatag ctgggcatcg gtattggtgc
     3541 cggctgcgga agatgtgacc ggaaatttgc gtccggcatt catgacactc ctggcggcgg
     3601 tgagcgctgt attactcatc gcgtgcagca acgtagcaaa cttattgctg gtgcgtttca
     3661 caggacgtcg gcgcgagatc gcgttgcgca tggcagtagg aggccagcga agcagcatcg
     3721 ttcggttgtt cgtactagag agcaccctgg tcagcgttat tgcgggtgtc gccggattat
     3781 gcctggcgct ttggacagta tcggttgttc cgcggatggc cgggcaaaac gttccgctgg
     3841 aaagtggcgt gtcattgcat ctgcccgtgc tcgctttcac attggggttg tcgctcatca
     3901 caggtctgct catgggtctt tacccggcct ggcagagttc gcgcgccgat ctggtcgaag
     3961 gactcaagga aggcggtggc ggaacgactg gtagtcatcg acaacatcgt ctccgtcgcg
     4021 gtttggtcgc agcgcaggtc gggctttcgg tcgcattggt ggcgagcgca gcgatgctga
     4081 tctcgagttt cgttcggctt agccggcagg aatccggctt tagatccgag cgcatttggg
     4141 cgggtggaat tgcgctgccg ccggcgcgat atcccgatcc tgcttcgcaa gctcgtttcg
     4201 tccaacgctt gaccacggaa ttacaaaacg agcccggtgt ggaaatcgca tccgttaccg
     4261 gcgcggtgcc gttgtctggg gacatatcca gggcgcctta cgcgcgggtt gatggaaatc
     4321 cattgccgtt gacccagcga ccgctcgggc tcacgcgcag cgtttcggcg aactattttc
     4381 gcacgctgag cattccactt gtttccggac gggactttag cgaacgcgac ggcgtcgatc
     4441 agccattggt cgtgattttg agcaaagcca cggccaaaaa acttttcccg aacgaggatc
     4501 cgttaggcca ccgcattctt tttggcaccg acaatggcac cggcgcacct gccgaagttg
     4561 tgggcatagt cggggacgtg cgatcggcag agttggccaa agcgaacgac gtcgaatttt
     4621 accggccctg ggcgcaacgc agtagtccat ttctgaatgt gctcgtgcgc agcacgacca
     4681 agccagaagc aaccaccgga attgtgcggc tcagtttgaa gaaaatcgac gatggcctgc
     4741 cgatcatcca accaaataca ctcgaggcaa tcgtcagcga ctcgctcggt gaccgacgct
     4801 tgacgatggc gctgctcggc gttttcgcgg gtatcgcctt gcttcttgcc gtggttggaa
     4861 tttatggggc cgtcgcgtac accgtcgagc agcggaccgg cgagattggg gtacgcatgg
     4921 cactcggcgc acagacaatc gatgtgcttc gcttggtcgt tcggcaggga atgaacccgg
     4981 ttattctcgg cttgatcatt ggtttggccg gaacatttgc tgttggccgt ctcctcgcgg
     5041 cccagctcta tcagatttcg ccacacgatc catttttgct cggcgctgcg gcaaccgtgc
     5101 tggccgtcgc cgcactgttg gcgtgtttgc ttccggcgcg ccgcgccaca cgtatcgacc
     5161 cgattcaagc actacgctcg gagtgatatg gatgatctaa ggttcgcttt gcgccagctc
     5221 cgcaaatctc cggggtttac ggctgtcgtc gtgatcacgc tcgcgctggg gattggcgct
     5281 aacgccgcca tcttcacgct cgtccgcggc gtgcttatca agcctctggt taaccgcgac
     5341 gaagaccggc tgatttatat ccggcagagt gcgacgggca tcggtaccga aaatagcacc
     5401 tggtcgatgc cggaaattgc ggacctaaag acgcgggtcg agaccctgag cgagttcggt
     5461 gacttttcca ccatcgcctt caccatgatc ggcctcggcg agcctcgtga actcaatggc
     5521 ggcgttgtca acgggtctta ttttgacgta atggggctac gtcccgcgct ggggcgcctc
     5581 atcgggccgg aagatgatgg cccgaaggct gcccccgtcg ttgtcctcac ctatcgtttc
     5641 tggtcgaacg ccctcaagcg agatccatcg gttattggca aaaccgttaa cctcggtggc
     5701 gttgagggcg atcgctccgc cacgatcatc ggcgtgctgg agccttgcgt gccgtatcct
     5761 caggagaccg aaatcatcgc caacgtagtc agcagcccac accatctctc ggcaaccatg
     5821 gtcacccagc gtgtccatcg catgacggaa ctttttgcgc atctcgcacc gggagccacc
     5881 ctcgatcaag ctcgggtcga attgcgttcc gtgtacgcca cgatgaaaaa agatcaccct
     5941 gaggcttact cgaagcaggc ggactttcag atctcggcca aactgcttcg tgaccagatt
     6001 acctcaggtg cgagaactgt gttactcgtt ctgctcgcag catccgccct cctgtttgtc
     6061 gttgcgtgct cgaatgtcgc gaacctcatc ctggcgcgta cggttcgccg cgaaggggag
     6121 ttggcggtcc gtaccgccct cggcgcaagc aaaggcgcgc tccgccgcat gttgctggcc
     6181 gaaagcctcc tgttgtgcgg gggcggcgcg gcgcttggcg tgctgagcgc gcagtggatg
     6241 gtcgacgtac tggctcgtta cgcctcgcgt ttctcggttc gtgccttgga cctcaccgtt
     6301 gattccagcc tgctgtgggt gggcgtgtcg ctcgccattg tcgccgcggt gattttggca
     6361 tttgtccccc gcctgcccgc tgacacgtcc ggtgcactgc atctctcaag cagcagcgcg
     6421 gcgttaagcg ggagcaagag gcgccagcgc atcttcgtcg ttacgcaaat cggcgcgtcg
     6481 tttttgctgc tggctggcgc gggtatggtg gtcacaacgc tcctcgcact tcagaggacg
     6541 cagaccggta tcgatacgcg ccgtgtcctg gccatcaacg ttccggccgt attttacgga
     6601 aaaacaggcc ggcaagttat cgatttttac aaggaagtga tacggcgcat tgatgcgctt
     6661 ccgggtgtca ataggaccgc cttcggaatg atcgctccat ggcgcgatgc cggctccggt
     6721 cccaccctgc aatttaacgc cgaagggtac gttaacgccg cgggcgaaga tgaccctcgc
     6781 gcgcagtatc gtgtcatctc tcccggattc tttgcggcgt tgggcgttcc tatcattgag
     6841 ggacgcgatt tcaatgatct tgacggtcga agtgaagaac cagtcgccat catcagccaa
     6901 accctcgcgc agcgcatgtt tcccaaccag gatccgatca atcggcacgt gttctggacc
     6961 gatccggtct tgaaacgcat cggcaagacg ccgccgcggc tgcaacgcat cattggcgtc
     7021 gccgcggata ttgacgacgt acatgtcgtt ccggagccca ccctgacgat ctatcacagc
     7081 tttgaacagg gtcccctgtt tcgcggccgc cttttcattc acgccagtgc aaatccctat
     7141 tcactgatca cgccggtcac gcgcattatt cgtgacttgt ccgtggatca gcccattgag
     7201 cgcgccgcta ccctcgaaga cgcccgcgcc gaagtgctta ctccgcaccg tttgaactcg
     7261 gtggtgtttg gtctgttcgc cgcagttgca ttgtcgattg ctgtggtcgg cgtcgcaggc
     7321 gtgctcgctt tttccgtcag cgcgcgcacg cgggaattcg gtatccggct ggcccttgga
     7381 tcgcagccga agcgtctttt caaagatgtc atcgtggagg gcgccgcaat ggccgcggcg
     7441 ggcatcatgg caggcgtaat attcggattc ctcctggcgc aggtggcgcg cagctatttc
     7501 gcggatgcaa cgatgcctgg cgttttgcca gtcgccgtgt cggcgtttgt gctgctggcg
     7561 gtcgccctgg ccgcgtcgat gctccccgcg caccgcgcgg cgcgcgtcga tgttattcaa
     7621 gcattacgca gcgaatgata tgaacgatct gaagttcgct ttacgccagc tcagcaaatc
     7681 accggcgttc actctggttg ctgtcctgac gcttgcactc ggcatcggtg cgaacactgc
     7741 catcttctcc gtcgtagaag gggtgcttct ccgtccactg cctttcccgc gcgctgaaca
     7801 actcgttagg atctacgaag ccctggatga aaatggtgcg cgcacggcgt cgctgaatct
     7861 gagcgatcgc acagtcgcgc gctttcgcga gttcggccgc gagattttcg aagatgttgc
     7921 tggtgggacc ggcggcgctg cagtcgttgg cgtgaacggt gcttcgcctg ttcagacggt
     7981 tcctgccgcc caggtcactt cgaacttttt cgatgtgctt ggattgcctc cagcgcaggg
     8041 tcggaatttc accgcagaag aaggactcaa tggaaccgcg agcgtcgcaa tcgtcagcga
     8101 cgacttttgg cgcaacacgc tgaactcgcg ccgcgacgtg ctcggcagct cgattgtgat
     8161 cgacggaacg ccgcgcacga tcatcggcgt gatgccgaaa gcattccggc atccctatcg
     8221 tgcgaatctg tggctgccac ttgcgcttca gcccgacaat ccggcgacga ttaacaacca
     8281 ttatctctat ggggttgcgc gactgcgtcc gggcgtcact cctcaaaaag cagaagacgc
     8341 agtgaagcgc atgtgcgcaa caatcaatcg cgacgatccg aatccggcaa atgcgcgcgc
     8401 tgcctacatc gtgccgttgc gcgagagctt tgtgatggat ttgcgtccga aaattctcgt
     8461 catcgtcggt gcggcagtct gtgccttgtt gatcgccgcg gcgaacttcg ccggtctgct
     8521 gctcgctcgc gtcgtcgagc gggaagggga gtttgccttg cgcgctgcgc tcggggcgag
     8581 caggctacga ctcgttcggc aacagctgac tcaggcgctc tcgctcgctt ccatcggcac
     8641 cgcgttcggt ctgcttatcg cattctggat cacgccgatg ctcttcgcgc tcagcccgga
     8701 aggcacggac gcaaccggga gcgcgatgcg tgaattcgat tacgctgcgc gacttgatct
     8761 gccggtcttc gctttcgctg cgggcgtgat gacgctggtc ggtttgggtt tcggtttgct
     8821 gcctgccgcg cgtgcatcgc ggaccgactt acgctgcgcg atgagcatca cgtcacgcgg
     8881 tgcaacactc gactgcaata cgcgacgatt gctcggttca ttcgtcgtga ttgagctggc
     8941 gattgcagct gcgctcctaa ccgcgagcat cactgcgaca caatattttt ggaagctgat
     9001 cgaggagcca tggggcttcg aaacgaaaga tcgcctcgcc ttcaacgtca ctgttcctga
     9061 tcgattcttt cccagcgcgg ccgcgaaaca gaacgcgctc gaagcttcgc tcgagcaact
     9121 gcgagcactg ccaggcgtcg aatccgcgac ggtcgtctcg ccttccccga tggacgcgtc
     9181 gtggacgttg atgctcttca acgtcgaagg cgcgcctgca cccgagccgc gcggtgttta
     9241 cacggcctac tcgcgcgtgc ccgtacccgg ctacttccag agtgtgggcc agccgatctt
     9301 gcagggccgc gatttcaaca ctagcgacac cgccgacgct ccgctcgtct gcattatcag
     9361 ccaaagtatc gcaaaacgct tttggcctaa cgattcgccg atcggcaaac gcatccgctg
     9421 gggacgactc gatggaactc gcccgtggtt cacaattgtg ggggtcgtcg gtgatatgaa
     9481 agcgatcgcg gatccgcgcg acggcgaagt gctcggcatg atcgcgcgcc cgctcgcgca
     9541 aatgcttgtg cacgcgacca cgcaactcga cgacatcacc ttcgttctgc atactgatcg
     9601 tcgctcggtg aacgagccgg ttatccgcgc cgcgctggcc cgcgcgaatg gaaatctcgc
     9661 cgcgtacaac tttcttgcgc tcgaagatgc cgcagcgcgc tcgcgcacga ccgagcgatt
     9721 catcttcgtc ctcgtatcat cgttcggatt cgtcgggctg atgctcgctg gtgtcggttt
     9781 gtatggcctg cttgcgctcc aggtcgcgcg gcgcgaacgc gagttcggca tcaggagtgc
     9841 actcggtgct accgcgcgcc agataatcca gctcgtcgcc cgacaaggcg ctaccttgct
     9901 ttcgttaggg tttactgccg gagcgctcgc gacttatgga gtcgtacacc tggtgcgaaa
     9961 tcaatggacc gaaatgcctg cgccgaatct ggtcgcgtgt atttgcgctg cgatagtgct
    10021 cggcatcgcg gtaatgatcg cgtcctggtt gccggcacgc cgcgctgcgc gtgttgatcc
    10081 tgtcattgca cttcgcgccg aataatatga acgatttaaa gtttgctttg cgccaactcc
    10141 ggaaatctcc cgcgttcact gcgattgcta tcatcacatt ggcgctcggg atcgggctga
    10201 acacggcgat ctttagtttg atcaacgatc tgttcctgcg cggacttccg tttcgacaac
    10261 cctcccgggt cctgcacgtc ttctcgcatg ccagagatca gcgggtcgat tttccacttt
    10321 cggcgccgcg tttcatgcac tttcgcgacg gccaaaccat ttttgacgga tttgctgggg
    10381 aaaacgggct tgcggccacc gtgactggcg tgggcgatcc gttccaagtt ccaatcttta
    10441 agacaaccgc aaattatttc gatgtgctgg gtgtgcgggt gatccgaggc cggacgtttt
    10501 cggctcagga ggaggaaggg gccgatgttg cggtcgtcac cgaacggttt tggaaatcgc
    10561 gccttggcgg cgatccgaat gtgatcgggc gcggactcac gctcgatgga gtgtcgcaca
    10621 ccatagtcgg tgtgataccg acgcttccgg tttcatggac cggaccgaat gccgatatct
    10681 ggacaacaaa gccgttcatc attccgggtt tttcctatga acggatgatg cggggtaccg
    10741 catttctgcg cgcggtcgga cgattgaagc ggggcatttc cgttatgcag gcgcgcgccg
    10801 cgctgcagtt gctcgaaaac agttatcgca cgcagtaccc agagaagatc gacgcaaaat
    10861 ccagtacgat agtgaagact ctgccggaag atgtaacgga aaatctgcga ccgggtttcg
    10921 ccacattgct cgcggcggta acctttgttt tgctgatcgc gtgcagcaac gtcgccaatt
    10981 tgttacttgt tcggttcact ggccgccggc gtgaaatctc acttcgaatg gctttgggcg
    11041 cgacgcgcgc aagtgtggtg cggctatttg ttttcgaaag cgttctgatc agcctacttg
    11101 cgggtatttt cggcgcggtt ctcgcgtggc aattcgtgcc ggttgtgccg agactcgcgg
    11161 cgaacttcct tccgattgaa cagggaagcg ccaccgcgat ttcctttcct gtgctcggat
    11221 tcacaattgc gctctcgctt ctgaccgggc tggtgatggg tctctatccc gcgctgcaaa
    11281 gttcgcgtgc tgagattgtc gatgctctaa aggaaggcgg ccgcgggact tccggcagcg
    11341 tgcaccaaca acgtttccgc aaattgctgg tcggcgcgca ggttgcactt tccgtaacgc
    11401 tactcgccgg cgcatcgttg ctcatagcaa gctttgttcg gctgagcagg caggatcccg
    11461 ggttcaggtt cgaaaatctc tggacgtgtt tcaccgttct gccagaagcg caatattcag
    11521 acgaaggaac acgagcccgg ttcgcagacc agctaacgaa aaccttgcgg gcaatgcccg
    11581 gcctgcagga agcgaccatt agcactggta ttccgctcac tggcggcgcc gcagctacgc
    11641 tttatgcccg tgcggacagc aacgtgccac ccgttgctga acgcaaaggc gctgcttcta
    11701 atgacatttc accaggatgg ttacgtacgc ttgggattcc gttacttgcc ggtcgcgact
    11761 tcaccgagca ggacgttgca gatcatccga acgtaattct catcagcgca tcgggagcaa
    11821 aaacggtatt tggaaatgaa aatccgattg ggaaaacatt gctcatcact agcggcagca
    11881 ttccggtcga gatcgttggc gtagtcggcg atgtgcgctc tgtgcgactc gatcagcgca
    11941 atgatatgga attttatcgc ccatacgcgc aggagaattt tccgtttctc tccatcacag
    12001 tgcggagttc cttgccgaca gatactgtca cgaaaaccgt gcagacggca ttgcgtacga
    12061 tcgatccgaa tctcgcgctc attttgccgc agccggtcaa agagatcatg cgacaggcgc
    12121 tcggccaggc aaaactaatg atggctctgt tgggaatttt cgccggcgtc gcgttgctgc
    12181 tcgctacaat tggaatctac ggtgccgtcg cttacacggt cgaacagcgg acaggcgaaa
    12241 ttggcgtacg catggccctt ggcgcgcaaa cggcggatgt gctgcggctt gttgttcgtc
    12301 agggaatggc tcctgtcgtg ttgggactcg tcatcggaat ggtggctgct cttgcgttgg
    12361 gtcgtttact ttctacgcag ctttaccaag tttcgcccca tagtccggcg cttcttgcta
    12421 ccacagcgct gactctcgga gcagtcgcat tgcttgcatg tttcctgccc gcgcgccgcg
    12481 caacatttgt aaacccaata gaagcccttc gggcagaatg acgaattaga tgcagacctt
    12541 tttccaggat cttcgcttcg gcttccgaat gcttcggcgg agtcccggct tttctctact
    12601 ggctatcctt tgcctgactt tgggcatcgg caccaatgcg gccgttttga gttggatcga
    12661 aggaattctg attcggccgt atccgctcgt ggcacatcag gaccggatgt tcgcgcttat
    12721 tggcacgacg cgcggcgtac cagggcataa cgggctctct tatcccgact tcgttgatct
    12781 ggaaaaaaac tccacgctgt ttgagtcgtt catcatcgat cgcatcaccg gcacgacgct
    12841 gagcgtcggc gaccgcgctg aacgcgcatc gctcggaatc gtttcggcca attatttcga
    12901 tgcactcggt gtgcggccga ttctggggcg cggattcagg cccgaagaag caactggacg
    12961 caatgcgcat cctgtcacgg tgattgctta tcggacctgg aaagatcgct acaatttcga
    13021 tccgaacatt gtcggccggg cgcaatttat gaacggtgtt cagcacacga ttgtcggcgt
    13081 cgcgccggaa aagttccacg gcacgttcgt gggatactct ttcagctttt gggtgccgac
    13141 ttcgatgcag gaggcgttcg acactacggg ctacaaattg gaagatcgcg gcgcgcgctg
    13201 gatcgagggc tacgcgattt tgaaacctgg cgtgtcacgt cagcaagcgc aggcccagct
    13261 gaattccatc tcacaacgct tggaaaagga ttttccggat acgaatcgcg gccacgagaa
    13321 ccagctcgtc ccattgtgga aaacgccgtt caacggcgcc gggaacatgt cgtctacgct
    13381 cgcgatcaca atggcggtgg tgtttttggt tttgctgatc gcgtgtgcca acgtcagtaa
    13441 tcttttgctc gcgcgttcgc tgttgcgccg gcatgagatg acgatgcggc tcgcgctcgg
    13501 cgccggccga ggccgtctgg ttaggcaatt ggtgaccgaa ggattattgc tttcgctgat
    13561 cgcagcactc ggtggaattg cagtcgcata ttggtgtcgt aatgcgctcg tgcttgcgtt
    13621 tccgccggcg ttgcctggaa tggtcatcga ctatcccggt caaatcgatt ggcgcgtgct
    13681 cgccgtgagc gccggaattt gcatggcatc gactctctta tttgcgctcg tgcccgcgat
    13741 tcgcgcgagc gatgtcgatc tttcaa
//
LOCUS       DASTDG010000038         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_314371, whole genome shotgun sequence.
ACCESSION   DASTDG010000038 DASTDG010000000
VERSION     DASTDG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314371"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1264)
                     /gene="gcvT"
                     /locus_tag="VFO30_01280"
     CDS             complement(86..1264)
                     /gene="gcvT"
                     /locus_tag="VFO30_01280"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9417947.1"
                     /translation="MLDLYLSLIDNYAGAVTETSAKKTPLYDEHVRRGAKMMPFAGWL
                     MPVQYTSIVEEHRAVRNDVGIFDISHMGQLTLEGSGAREWLNVMLTNNIEKLEVGTGQ
                     YTFLLNDGGGIIDDLIVYRTDDQKFLLVVNASRTDEDFVWLQKWHRLTADVYRNHRQD
                     ADAMTRLTNRSGEFGGVAVQGPRVVDLLRALNVDLPPRNHIVDLTIERMPLSIARTGY
                     TGEDGVEVFFRAEDAAKVWNLVLETGERFGIKPCALGARDTLRLEMCYPLNGSDLSPE
                     HNPIEAGLGVFVDLAKPNFIGRDVLLQTKENGPREKLVPFRMKEKGPPPRPHYAVFGN
                     GERIGEVTSGTLSPSLQRGIGMAYIDAPRAKVGAKIDIEIRRQKFPAVIEKKPLYKKT
                     "
     gene            1387..2169
                     /locus_tag="VFO30_01285"
     CDS             1387..2169
                     /locus_tag="VFO30_01285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9417948.1"
                     /translation="MSWLDKLERRLGFLAVPGLIRIIVGISALVFFLVRLNPDLRFVF
                     DLDPQRIRHGEVWRLLTYIFIPQTFSFLWILFALWFLWFVGDGLERAWGSFRLTLYVI
                     VGMIGTTAAAFFFGSNFSNSMLIASLFFAFARFYPNEIIYAFFILPVKIKWLAWVSAA
                     LLLLGFFVNSNSYRMALVAALSNYLIFFGPEIVYEARHRREVSTRRRRFEIHSRSEAE
                     PLHKCKVCGATEMSDPNLDFRVARDGEEYCMAHLPRAQTPAR"
     gene            2254..3069
                     /gene="hemQ"
                     /locus_tag="VFO30_01290"
     CDS             2254..3069
                     /gene="hemQ"
                     /locus_tag="VFO30_01290"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HET9417949.1"
                     /translation="MTDKSAALVPEEGWHCLHLFYRVEHGQWQLFSREEQNAAKTNLA
                     RLVQEIRALESTQLLTLSIVTPKADLGFMLITPDLQMANKIDKQLSLSLGPDVLTPVY
                     SYLSLTEESEYITTAEEYAHGLDEETRRDATKLEQALATFRERMKHYRQERVYPTLPD
                     WPVVCFYNMSKRRGEQRNWYALAYEDRRKLMKGHAAVGREFAGRVKQLITGSTGLDDA
                     EWGVTLFARDTFQIKAIVYKMRFDLVSAEYAEFGEFFIGIQLPLDELFRRLQL"
     gene            3189..3279
                     /locus_tag="VFO30_01295"
     tRNA            3189..3279
                     /locus_tag="VFO30_01295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3223..3225,aa:Ser,seq:gga)
BASE COUNT          661 a          978 c          905 g          777 t
ORIGIN      
        1 tgatcggtga taccgacgcg gatgttgtcg ccctcgcgac gaatccattc gtgcgtttcg
       61 gtgtaaagca aatcgtccgg aatgttcacg tctttttgta gagcggtttt ttctcgatca
      121 cagccgggaa tttttgccgg cgtatttcga tatcgatctt cgcgcccact tttgcacgcg
      181 gcgcatcgat gtaagccatt ccgatcccgc gctgcaaact tggcgagagc gtgccgctcg
      241 taacttcgcc aatgcgttcg ccgttcccga ataccgcata gtgcggccgc ggcggaggac
      301 ctttctcttt catgcgaaac ggcacgagct tttcgcgcgg gccgttctct ttcgtttgca
      361 acagaacgtc acggccgatg aagttcggct tcgcaagatc gacgaaaacg ccgaggcctg
      421 cttcgatcgg gttatgttcc ggcgacaaat cggacccgtt cagcgggtag cacatttcca
      481 gtcgcaaagt gtcacgcgcg cccagggcgc acggtttaat cccgaatcgt tctcctgttt
      541 ccagcacgag attccaaacc ttcgccgcgt cctccgcgcg aaagaaaact tccacgccat
      601 cttcgcccgt gtaaccggtg cgtgcgatcg agagtggcat ccgttcaatt gtgagatcaa
      661 caatgtgatt gcgtggtgga agatcgacat ttaacgcgcg aaggagatcg acgacgcgcg
      721 gaccttggac ggccactccg ccaaactcgc cgctacgatt tgtaaggcga gtcatggcat
      781 cggcatcttg ccgatggttt ctgtaaacat cggctgtaag ccgatgccac ttttgcagcc
      841 agacgaaatc ttcatccgtt cgcgacgcat tgacgacgag cagaaatttc tggtcatcgg
      901 ttcgatagac aatcagatcg tcaataatcc cgccgccgtc gttgagcaaa aaagtgtact
      961 ggccggtgcc gacctccagc ttctcgatgt tgttggtcag catgacgttc aaccattcgc
     1021 gcgcgccgct gccttcgagg gttaactggc ccatgtgcga aatgtcgaaa atgcccacgt
     1081 cgtttcgcac cgcgcgatgt tcctccacga tgctcgtgta ttgcaccggc atcagccagc
     1141 cggcgaacgg catcattttc gcgccacggc gcacgtgctc gtcgtaaagt ggagttttct
     1201 tggctgatgt ctcagtcacc gcaccagcgt agttgtcgat caaactgagg taaaggtcga
     1261 gcacaactgg agcggccgct cctcgcggcc taaacaagtg cgaactgcga ccaccacaat
     1321 ttaccaacgc gcgccgagat cgcgttccat cttgccagca aaacgttgcg ttcttaccgt
     1381 gcgcccgtgt cctggctcga taaactcgaa cgccgtctcg gcttcctcgc cgtgcccggc
     1441 ctcatccgca ttatcgttgg gatcagcgcc ctcgtcttct tcctcgtccg actcaatccg
     1501 gatttgcgct tcgtttttga tctcgatcca cagcgcatcc gccacggtga agtctggcgt
     1561 ctgctgactt acattttcat cccgcagaca ttcagctttc tctggattct gttcgccctt
     1621 tggtttctct ggtttgtcgg cgacggcctc gaacgcgctt ggggctcctt ccggctcacc
     1681 ctttacgtca tcgtcgggat gattggcacg accgcagcgg cgttcttttt cggcagcaat
     1741 ttttctaatt cgatgttgat cgcctcgctc ttctttgcgt ttgcgcgctt ttacccgaac
     1801 gaaatcatct acgcgttttt tattttgccg gtgaagatca aatggctggc ttgggtttcg
     1861 gcggcccttc ttttgctcgg cttttttgtc aactcgaact cgtatcgcat ggcgctggtc
     1921 gcggcattga gcaattatct gatcttcttc ggtcccgaga ttgtttacga agcgcgtcat
     1981 cgccgtgagg tttcaacgcg gcggagacgg ttcgagatcc actcgcgcag cgaggccgag
     2041 ccgctccaca aatgcaaagt atgcggcgcg actgagatga gcgacccgaa cctcgatttc
     2101 cgcgtggcgc gcgatggcga ggaatattgc atggcccatc tgccgcgtgc acagacgccc
     2161 gcgcgctaga gtagggcaag cgtctcgcgt gcgaattgcg agtcgcaacc gggacggttg
     2221 cgctactatc ctctacgaca gaaactagcc gccatgactg ataagagcgc agcacttgtg
     2281 ccggaagaag gctggcattg tctgcatctg ttttaccgcg tcgagcatgg acaatggcaa
     2341 ttattcagtc gtgaggaaca gaacgcggcg aaaaccaatc tcgcgcggct cgttcaggag
     2401 attcgcgcgc tcgagtcgac gcaattactc acgctcagca tcgtcacgcc gaaagccgat
     2461 cttggcttca tgctgatcac gcccgatctg cagatggcaa acaagatcga caagcaacta
     2521 tcgctttcat taggcccaga cgtgctcacg ccggtttaca gttatctctc cctcactgag
     2581 gaaagcgaat acatcaccac tgcagaagaa tacgcccacg gcctcgacga agaaacacgg
     2641 cgcgacgcga ccaagctcga gcaggcgctg gcgacgtttc gcgagcgcat gaaacattac
     2701 cgccaggagc gcgtttaccc cacgctgccc gattggccgg tggtttgctt ctataatatg
     2761 agcaagcgcc gcggcgaaca gcgtaattgg tacgcgctgg cttatgagga ccgtcgcaag
     2821 ttgatgaagg gccatgccgc agtcggccgc gagttcgcgg gcagagtgaa acagctcatc
     2881 actggctcga ccggccttga cgatgccgag tggggcgtga cgctcttcgc gcgcgacacg
     2941 ttccagatca aagccatcgt ttacaaaatg cgcttcgatc tggttagcgc cgagtacgct
     3001 gagttcggtg agttcttcat cggcattcag ttgccgctcg atgagctctt ccgccggctg
     3061 caactctgag cggcgagttc gcacctgtag gggacgctgc tagcgtccca actgaaaccg
     3121 ggaagctgac agcttcccct acaatgattc tcgattagat tgtcgagctt ctgcgtttgc
     3181 cagccaccgg atgggtgaca gagtggtcga ttgtgcacgc ctggagagcg tgtgtgcccc
     3241 aaaagggcac cgagggttcg aatccctccc catccgccag ctgaggaact ttgcggatca
     3301 ggctcgaatc agctgccttc t
//
LOCUS       DASTDG010000039         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_397702, whole genome shotgun sequence.
ACCESSION   DASTDG010000039 DASTDG010000000
VERSION     DASTDG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397702"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2235)
                     /gene="glgB"
                     /locus_tag="VFO30_01300"
     CDS             complement(4..2235)
                     /gene="glgB"
                     /locus_tag="VFO30_01300"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET9417950.1"
                     /translation="MKPFSVSGLPPDELNSFLSGTHSDPFRILGPHRAGDDLIVRVFR
                     PDAKKIDILLAAASPSGGGQDRQRIEAEKVHRDGFFCATVPGATRDLDYRIQVTTWEG
                     AEQVARDAYQYGPIMGEIDLHLFTEGQHWELYERFGAHLRTIGDQRGVYFAVWAPNAQ
                     RVSVVGDFNGWDGRVNPMRKLLGAGVWELFIPGVGEGAHYKFEIRTYPGALLLKADPF
                     AFFSQHGTSTASLVYDLSRYEWNDGEWMDLRRTRDWPKSPINIYEVHLGSWRRRADEG
                     NRFLSYIELAETLIPYVVEMGYTHIELLPIAEHPFEGSWGYQVTNYYAPTSRYGNPDE
                     FRHFVDKCHQAGIGVIMDWVPGHFPKDAYALAEFDGTDLYEHMDPRQGEQQDWGTLIF
                     NYGRNEVRNFLIANALFWLDKYHIDGLRVDAVASMLYLDYSRKPGQWIPNVFGGRENL
                     DAIYFLKRFNEICYERFPGIITIAEESTAWPGVSRPTYLGGLGFGFKWNMGWMHDFLE
                     YMSLDPIYRRFHHGNITFSLLYAFTEHFILVLSHDEVVHGKRSLLSKMPGDEWQKFAN
                     LRMFYAWMCGQPGKKLLFMGGEFGQWNEWNHDTSLDWQLVDLPRHDGLRRLVQHLNFT
                     YKNEAALWEQDDTYEGFEWIDFHDADNSVVSFMRKSRAGETVVFVVNATPIVRYDYRL
                     GVPKAGFYRELINTDAETYGGSNVGNYGGVQSEDRQWMGREHSILIQLPPLATLAFKF
                     EKL"
     gene            2381..3304
                     /gene="epsC"
                     /locus_tag="VFO30_01305"
     CDS             2381..3304
                     /gene="epsC"
                     /locus_tag="VFO30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9417951.1"
                     /translation="MQNTEQSAVDELLQTYSETGGINYLAAAAMLPSRLAVEEVCTEL
                     MSLMFPGFRSETVVNSEDLAEITRARIRNLHARLKTEICRSLGKVPPEEKTERRAEEV
                     LAVFLGELPHVRRILWSDIDAAYEGDPAARSYEEIILAYPALEAVAIYRMAHVLYDKV
                     PLIPRIMTEWAHSRTGIDIHPGAKIGPNFFIDHGTGVVIGETSEIGSRVKLYHAVTLG
                     ARSFQKDEHGKIKKGGKRHPTVEDDVTIYPGSTILGGETIIGARSTIGGNVFLMQSVP
                     PDSLVYYEEKQLRIMPKKPHKSAGPRAAFTG"
     gene            3301..>3562
                     /locus_tag="VFO30_01310"
     CDS             3301..>3562
                     /locus_tag="VFO30_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9417952.1"
                     /translation="MKSGTSVPLGFDLVRKPEARATVIYLDYNATTPLCDEARQAMLP
                     YLDRFFGNPSSVHVAGREARAAIDNSRDKLAALLRVKPNELVF"
BASE COUNT          788 a         1043 c          998 g          733 t
ORIGIN      
        1 cctctacaat ttctcgaact taaatgccag cgtcgcgagc ggcggcaact gaatgagaat
       61 ggaatgctca cggcccatcc attgtcgatc ttcgctttgg acgccgccgt aatttccgac
      121 gttactgccg ccataggtct ctgcgtcggt gttgatgagc tcccgataga acccggcctt
      181 gggcactcca agccggtaat catacctaac aattggcgtt gcatttacga caaacacaac
      241 tgtttccccg gcgcgtgatt tgcgcatgaa ggaaacgacg ctgttatccg cgtcatggaa
      301 atcgatccac tcgaagcctt cataggtgtc atcctgttcc cataacgccg cctcgttctt
      361 ataggtgaag ttgagatgtt gtacgagccg gcgcaggcca tcgtggcgcg gaagatcgac
      421 aagctgccag tcaagactcg tgtcgtgatt ccactcgttc cattggccga actcgcctcc
      481 catgaagagc agtttcttcc ccggctggcc gcacatccag gcgtaaaaca tccgcaggtt
      541 cgcgaacttc tgccattcgt ctcctggcat cttagaaaga agcgagcgct tgccgtggac
      601 tacttcgtcg tggctgagca cgagaatgaa atgctcggtg aacgcgtaga gcagcgaaaa
      661 ggtgatattg ccgtgatgga accggcggta aatcgggtcg agcgacatgt attcgagaaa
      721 atcgtgcatc caacccatgt tccatttgaa gccaaagccg aggccgccga gataggttgg
      781 tcgcgacacg cccggccagg cggttgattc ctcggcaatg gtgatgatcc cgggaaagcg
      841 ctcgtaacag atctcattga agcgtttcag gaaataaatc gcgtccaagt tctcgcgacc
      901 gccgaaaacg ttcgggatcc attggccggg tttgcgcgag taatcgagat acaacatgga
      961 cgcgaccgca tccacgcgca ggccatcgat gtgatatttg tccagccaga agagtgcatt
     1021 cgcgattagg aaattccgca cctcgttgcg gccatagttg aaaataagcg ttccccaatc
     1081 ctgctgctcg ccctggcgcg ggtccatgtg ctcgtaaaga tcggtgccat cgaactcggc
     1141 cagcgcgtac gcgtctttcg gaaaatgccc tggcacccaa tccataatca caccgatgcc
     1201 ggcctgatgg catttgtcga cgaaatgacg gaactcatcg ggattgccgt agcggctggt
     1261 cggcgcgtaa tagttcgtga cctgataacc ccacgaacct tcaaatgggt gctcggcaat
     1321 cggcagcagc tcgatgtgcg tgtagcccat ctcgaccacg tacgggatca gcgtctcggc
     1381 caactcgata taactcagga accgattccc ctcgtccgcc cggcgccgcc aggagccgag
     1441 atgcacttcg taaatgttga tcgggctctt cggccaatct cgcgtgcggc gcaggtccat
     1501 ccactcgccg tcattccatt cgtagcgcga cagatcgtaa acgagcgagg cggtcgatgt
     1561 cccgtgttgt gagaaaaacg cgaacggatc ggctttgagc aaaagcgcgc cggggtaggt
     1621 gcggatctcg aacttgtaat gcgcgccttc gccgactccc ggaatgaaca gttcccaaac
     1681 gcccgcgcct aacaacttgc gcatcgggtt gacgcgaccg tcccagccgt tgaaatcgcc
     1741 gaccacactg acgcgctgcg cattcggcgc ccagaccgca aagtaaacgc cgcgctggtc
     1801 accgatggtt cgcaaatgcg cgccaaacct ttcgtaaagc tcccagtgct gcccctcggt
     1861 aaacagatgc aggtcgatct cgcccatgat cgggccgtat tgataagcgt cacgtgcgac
     1921 ttgctctgcg ccctcccacg tcgttacctg aatgcggtag tcgagatcgc gcgtcgctcc
     1981 cggcacggtc gcgcagaaaa atccgtcgcg gtgaactttt tcggcctcga tgcgttgtcg
     2041 atcttgtccg ccgccggacg gactcgctgc ggcgagcaag atgtcgatct tctttgcgtc
     2101 aggtcgaaac acgcgcacga tcagatcgtc accggcgcga tgcggcccga ggatgcggaa
     2161 cgggtccgaa tgcgtgccgg aaagaaagct gttcaactca tccggcggca ggccggaaac
     2221 ggagaaaggt ttcatgcgat tcgttgaagg gttaaagcgt tgaaccgttg aagcgagcca
     2281 acccttctat cgcttcaacg cttcatcact tcaacccagt tcatcgcttc aactcttgaa
     2341 cgctggattt gaccgccaac gcgtcgcata gttcatcggg atgcaaaata cggagcagtc
     2401 ggcggtcgat gagctgctgc agacctatag cgaaaccggc ggcatcaatt atctggcggc
     2461 ggcggccatg ctgccctcgc ggctggcagt cgaggaggtc tgtaccgagt tgatgagcct
     2521 catgtttccc gggttccgca gcgagactgt cgtcaactcg gaggacctcg ccgaaatcac
     2581 ccgggcgcgg atcagaaatt tgcacgcgcg actcaagacc gaaatttgcc gcagcctggg
     2641 caaagtgccg cccgaggaaa aaacggagcg ccgggccgag gaagtgttgg ccgtgttttt
     2701 aggcgagctg ccacatgtgc ggcggattct ttggagcgac atcgacgcgg catacgaggg
     2761 cgacccggcg gcgagaagtt atgaggagat catcctcgct tatcccgcgc tcgaagcggt
     2821 cgccatctat cgcatggcgc acgtcttgta cgataaggtg ccgctcatcc cgcggatcat
     2881 gaccgaatgg gcgcacagcc ggactggcat cgacattcat cccggcgcaa agatcggccc
     2941 aaatttcttc atcgatcacg gcaccggcgt ggtgatcggc gagacctccg aaatcggttc
     3001 gcgcgtaaaa ctttatcacg cggtcacact cggcgcgcgc agctttcaga aagatgagca
     3061 cggcaaaata aaaaagggcg gcaagcgtca tcccacggtc gaggatgatg tcaccattta
     3121 ccccggctcg actatccttg gcggtgaaac catcatcggt gcaagatcga caatcggcgg
     3181 taacgttttt ctcatgcaaa gcgtcccgcc cgattcgctc gtctactacg aggagaaaca
     3241 actgcgcatt atgccgaaaa agccgcataa atcggccgga ccacgagccg cgttcaccgg
     3301 atgaaaagtg gcacaagcgt cccgcttggg tttgatttgg ttcgcaagcc tgaggctcgc
     3361 gctaccgtaa tctacctcga ctataacgcc acgactccgc tgtgtgatga agcgcggcaa
     3421 gcgatgctgc cgtacctcga tcggtttttt ggaaaccctt ccagcgtaca cgtcgcggga
     3481 cgcgaagcgc gcgccgcaat cgataattcg cgcgacaagc ttgccgcact tcttcgggtt
     3541 aagcctaacg aattggtttt ta
//
LOCUS       DASTDG010000040         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_397622, whole genome shotgun sequence.
ACCESSION   DASTDG010000040 DASTDG010000000
VERSION     DASTDG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397622"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFO30_01315"
     CDS             complement(<1..281)
                     /locus_tag="VFO30_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417953.1"
                     /translation="MDPQLRSTFNAGFTPAKYAALLRCVNKTEKWPADFRIAETPIFL
                     TREFTDEVVRAAKEIVDLTRTADFKRLARDAVPRSLQVPNESAHPNFFVV"
     gene            complement(302..520)
                     /locus_tag="VFO30_01320"
     CDS             complement(302..520)
                     /locus_tag="VFO30_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417954.1"
                     /translation="MIADVRERLERVPFVPFIIRTSDGREYSVPTTDHAHIHPRGHRV
                     IVFTDEGATAVLGPLHINSVGEQQSDGK"
     gene            complement(529..1548)
                     /locus_tag="VFO30_01325"
     CDS             complement(529..1548)
                     /locus_tag="VFO30_01325"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9417955.1"
                     /translation="MPDRLTVDTIDTELAPGARNAVSVCLQLTPGERVTIITDEATVE
                     IAAALRLEVEAIGSEHSVFILENYTSRPLKFMPEPILYDLAKSQVSIFCAQSQPGELP
                     SRMQMSDVVNKHHIRHAHMVNINRQIMLEGMRADFREVDALSRRLIERARHAERISCR
                     TPHGTKFEAEFSPKLKWLKTSGIITPEKWGNLPGGEIFTAPANTNGTFVVDGVVGDYL
                     CTKYGDLRDAPLTIQVADNRIVDLRCKNRELLDDFRAYTSTDENSNRVGEFAIGTNTA
                     LTRVIGNILQDEKIPGVHIAFGHPYAEHTGADWKSKTHIDCVGRDFDVWFDGEQVMRG
                     GQFLP"
     gene            complement(1596..2726)
                     /locus_tag="VFO30_01330"
     CDS             complement(1596..2726)
                     /locus_tag="VFO30_01330"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET9417956.1"
                     /translation="MGAKDHTYTLGIEEEFAIVDPETRELKSHIQEILEGGKVVLKEQ
                     IKPEMHQSVVELGTEICESITNARAHVVDLRSKLAKLAGNAGLKIASVGTHPFSHWRD
                     QLITEGERYREIVKDMQQLARANLIFGLHVHVGIPDRASAINVMNQARYFLPHIYALS
                     VNSPFWVGQDTGLKGYRLKVFERFPRTGIPDAFESLSEYEDYCKLLVKTGCVDNAKKI
                     WWDIRLHPFFDTLEVRVCDAQSRVDDTLAIAALIQAVIAKLYKLLRQNTTFRVYRRRL
                     LDENRWRATRYGIDGKLIDFGKETEVETRSLLNELLEFVSTEVNELGSQREMAHIERI
                     MHEGTGADRQLATFARTNEMKAVVDQIVDETYEGLNLNAFAG"
     gene            complement(2824..3789)
                     /locus_tag="VFO30_01335"
     CDS             complement(2824..3789)
                     /locus_tag="VFO30_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417957.1"
                     /translation="MKKIGILFGQEHSFPPAFVERVNQKTGGRDIVAEFVRIDKVMQG
                     EPCGYDVVIDRISQDVPFYRAWLKNAALTGTAVVNNPFWWSADDKFFNNALATKIGVA
                     VPRTVLLPSNQPPPDTNEKSFRNLAYPLNWEEIFAYVGWPAFFKPFAGGGWKNVYRLH
                     NRDEFYRAYNETGQLVMMLQEEVKFEMYFRCYSIDQRHVRIMPYEPRHPYHLRYQTEW
                     QTPPEILQKVEQGVLTLNQFLGYDLNTVEFAIRDGVPVAIDFCNPAPDAEAANVGQAN
                     FEWVVEAVSEMAIRKAREHVSGRDNLSWGKFVQAAVTHRSLGELG"
BASE COUNT          920 a         1202 c         1186 g         1004 t
ORIGIN      
        1 accacgaaaa aatttggatg cgccgattcg ttaggcactt ggagtgatct cggcacggca
       61 tctcgggcaa ggcgtttgaa gtcggcggtg cgcgtaagat cgacaatctc cttcgctgcg
      121 cgcacgacct cgtcggtaaa ctcccgcgtg aggaagatcg gcgtctcagc aatccggaaa
      181 tcggccggcc atttctccgt cttattgacg cagcgaagca gcgcggcgta tttcgccggc
      241 gtgaaaccgg cgttaaaggt cgagcgaagc tgcggatcca cgtttcgcga tccgccgcag
      301 gttacttacc gtccgactgc tgctcaccca cgctgttgat atggagcggg ccgagcactg
      361 cggtggcacc ttcgtcggtg aacacaatca cccggtggcc gcgtggatga atatgggcgt
      421 gatcagtcgt tgggactgaa tattcacgac catcggaagt tcgaatgata aacgggacga
      481 acggaacccg ctcgagccgc tcccgcacat ccgcgatcat aggtcgaact atggcaaaaa
      541 ctgtccgccc cgcatgacct gctcgccgtc aaaccagacg tcgaaatcgc ggccgacgca
      601 gtcgatgtgc gttttcgatt tccaatcggc gccagtgtgt tccgcataag ggtggccaaa
      661 ggcgatgtgg acgccgggaa ttttctcgtc ctgcaaaatg ttcccgatga cgcgcgttaa
      721 ggcggtgttc gtgccgatgg caaattcgcc gacgcggttg ctgttctcgt cggtgcttgt
      781 gtacgcgcga aagtcgtcga gcaattccct gtttttgcag cgaagatcga caatgcgatt
      841 gtcggcaacc tggattgtta gcggcgcatc gcgaagatct ccgtacttgg tgcaaagata
      901 gtcgccgacc acaccatcga ccacgaacgt gccattggtg ttcgccggcg ccgtgaagat
      961 ttcaccgccc ggcaggttgc cccatttttc cggagtgatg atgccgctcg ttttgagcca
     1021 ttttagtttt ggcgagaact cagcttcaaa tttggtgcca tgcggtgtac gacaactgat
     1081 gcgctcggca tgacgcgcgc gttcgatcaa tcggcgacta agcgcatcca cttcgcggaa
     1141 atcggcgcgc atgccttcca acatgatctg ccggttgata ttcaccatat gagcgtgacg
     1201 gatgtgatgc ttattcacca cgtcgctcat ttgcatgcgc gaagggagct cgcctggctg
     1261 actttgcgcg caaaagatcg acacctgcga tttagcaaga tcgtagagaa tcggttctgg
     1321 cataaacttg agcggacgcg acgtgtagtt ttccaaaatg aagacggagt gttcggaacc
     1381 gattgcttcg acttcaagtc gcaatgcggc tgcgatctca acagttgctt cgtccgtaat
     1441 gatcgtgacc cgctctcccg gcgttagctg aaggcaaacg cttacggcat tgcgcgcacc
     1501 gggcgcgagt tccgtatcaa ttgtgtcgac agtcagacgg tcaggcattt tggaaggtgc
     1561 tcagttttat gcggaaattc gagtcgagca aaccatcaac ctgcaaatgc attcaaattt
     1621 aatccttcgt acgtttcatc cacgatttga tcgacaacgg ctttcatctc gttcgttcgc
     1681 gcgaatgtgg cgagctggcg gtccgcgccg gtgccttcgt gcataatacg ctcgatgtgt
     1741 gccatctcgc gctgactgcc gagctcattt acttcggtcg aaacaaactc gagcagttcg
     1801 tttagcagac tgcgcgtttc gacttcggtc tctttgccga agtcgatcaa tttgccgtcg
     1861 atgccgtagc gcgtcgcgcg ccaccggttc tcatcgagca agcgacggcg atagacccgg
     1921 aaggtggtat tttgtcgcag taatttgtaa agtttggcga tcacggcttg aatcaacgcc
     1981 gcaatcgcga gggtatcgtc gacacgcgat tgcgcgtcgc acacgcgcac ctcgagcgtg
     2041 tcgaagaaag gatggagccg aatgtcccac cagattttct tggcgttatc gacgcagcca
     2101 gttttcacca acagcttgca gtaatcttcg tactcagaga gcgattcgaa cgcgtcggga
     2161 attcccgtgc gcggaaaacg ctcgaacacc ttaagccgat aaccttttaa gccggtgtct
     2221 tgtccgaccc aaaatggtga gttgactgag agcgcgtaga tgtgcggcag aaaatagcgc
     2281 gcctggttca tgacgttgat ggccgatgcg cgatccggaa tgccgacgtg cacgtgcaaa
     2341 ccaaaaatga ggttcgcgcg cgcgagctgc tgcatgtctt tcacgatttc acgatagcgc
     2401 tcgccttcag tgatgagttg atcgcgccag tgcgaaaacg gatgcgtgcc gacggacgcg
     2461 attttcaggc cggcgttccc ggcgagcttc gctagtttgc tgcgaagatc gacaacgtgc
     2521 gcgcgcgcgt tggtgatcga ttcgcaaatc tcagtcccga gctcgaccac cgattgatgc
     2581 atctccggtt tgatctgctc cttgagcacc accttgccgc cttcgagaat ttcctggatg
     2641 tgcgacttca gttcgcgcgt ctccggatcg acaatcgcga actcttcctc gattccgagg
     2701 gtataggtgt ggtccttggc gcccatcgca gagcgcaaat ctctctcaaa aacaatgcgc
     2761 tgtcatgcca tgtgggagcg gtcgcagcgc cgcgacggtc gggacgctaa gctccctccc
     2821 acattaccct agctcaccta acgagcggtg cgtgactgcc gcttgcacaa acttgcccca
     2881 gctcaggttg tcccggccgg acacgtgttc gcgtgctttg cggatggcca tttccgaaac
     2941 ggcttccacg acccattcga agtttgcctg gccgacattg gcggcctccg catctggcgc
     3001 gggattgcag aaatcgatcg ccaccggcac gccatcgcga atcgcaaact caaccgtgtt
     3061 cagatcgtag ccgagaaatt gattcagggt gagcacgcct tgctcgactt tctgcaaaat
     3121 ttccggcggg gtctgccatt cggtttgata acgcaaatga tacggatgcc gcggttcgta
     3181 gggcatgatc cgaacgtgac gctgatcaat cgaataacag cggaagtaca tctcgaactt
     3241 cacctcctcc tgcaacatca tcaccagctg gccggtctcg ttgtaggccc ggtaaaattc
     3301 gtcccgattg tgcagccgat acacattttt ccagccgccg ccggcgaacg gcttgaaaaa
     3361 cgccggccag ccgacataag cgaaaatttc ttcccaattc aacggatacg cgagattgcg
     3421 aaatgatttc tcgttcgtgt ccggcggcgg ctggttcgag ggcaataaga ctgtgcgcgg
     3481 tacggccacg ccgatttttg tcgcgagcgc gttattgaaa aatttgtcgt ccgcgctcca
     3541 ccagaacgga ttgttcacca ccgcggtgcc ggtgagcgcc gcgttcttca gccaggcgcg
     3601 atagaacggc acgtcctgcg aaatgcgatc gattacgaca tcataaccac atggctcgcc
     3661 ctgcatcacc ttgtcgatcc tgacgaactc ggccacaatg tcgcgcccgc ccgttttctg
     3721 attcactcgc tcgacaaagg ccggcggaaa actgtgttcc tgcccgaaaa ggattccgat
     3781 ttttttcata gcttgcgttc tgtcatttcg agcgaagtcg agaaatctct tactattatc
     3841 tgtcatcaag cacggcagcg gcgagatcaa tccagctcgg attgaatcga ttgatcaggt
     3901 caactttctt tgctcgtgac catttcttta actgcgattc acgcgcgatc gcttcgtgga
     3961 cgttctcgta attcgtaata gatgagttta ttgcagcggt aagcagctgg aaaattagcg
     4021 ccagtgcctt gacggtgctc ccaagctctg cgcgacaggc tgtttgtcag tccgatatac
     4081 aacacagagt gatttcgatt tgtgatgatg tagatccaaa agtcgtattc ccgcggcatc
     4141 tcaggctagc aataagagat gtctcgactt cgctcgacat gacaggaaga gttcacagcg
     4201 tcgacaaata gtagggcagc atgtcgcgcc agtagctcca ctcgtggccg caccatttgc
     4261 ggtcgtcgag ccagtggggg atgcctttgg aattcaaaat ctcggcgagc cg
//
LOCUS       DASTDG010000041         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_431279, whole genome shotgun sequence.
ACCESSION   DASTDG010000041 DASTDG010000000
VERSION     DASTDG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431279"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VFO30_01340"
     CDS             <1..1531
                     /locus_tag="VFO30_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9417958.1"
                     /translation="LSKIAELKVISRTSTQKYKSTPENLREIGQQLGVAHFLEGSVQK
                     VANAVHVNVQLIRAATAEHLWAESYNRKLDDVFGVEGEVASAIAEQLNAKLTGAEQKA
                     VADTPTQNAAAYDVYLRAVAIDNALTLDTTKRVADLYAEAVRLDPQFALAWARLAVAR
                     SQLYFNGLDLDKNSGAAVKDAADRAMSLQPELGEAWLAQGVYRYRVLRDFQGALRSYE
                     EALRRLPNSAFVLQQMAHLERRLGQADVAQKHYQAAAQLDPRNIGILLTLADTFRSVR
                     RYDEARAVLDRALEMAPGNEAALGVKALTFQDEGRLNEAAEILAKAPANSQDEALIFA
                     RVAQFYDERRFDEAIAQIQQNTPAALANDPRTLTLLGYCQKFAGHSDEARATFTRAAA
                     AMKPTPDSVVVIDARNLPCYLAWVYAGLGEKEKALEQARHAIADYYSDALVKPFAETS
                     LAIVEAQTGDIDSAIASLPHLLEMPNGETCSDLQLNPLWDPLRKDPRFQKLCSGALSE
                     N"
     gene            complement(1542..2483)
                     /locus_tag="VFO30_01345"
     CDS             complement(1542..2483)
                     /locus_tag="VFO30_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417959.1"
                     /translation="MRIWCASLSIFTSAIIASACADDFPIKRFSPFTYVVDYEASWSP
                     DSRKIALVSSRHGGMKVYAMNADSASHGSDMRQLTFGSDEDDSPAWSPDGKKIAFVSI
                     RAGVSEIFVMNADGTNVRQLTNGNAENIHPTWAPDSQRILFNTTKFAVATAADGRDVP
                     SDNKVIGEQIDKKMDLATIRTDGTELKRLTTGGGYTYASFSPDGSSILHRRVDGGKSQ
                     IFLMNADATNDHNISGKNTLDGWPAWSNDGKRVVFSRRVNDRFQVFTMNRDGSGAMQL
                     TDAAGEFVNPRWSPDGTKIICARRLGDMNLVMFPAPK"
     gene            2539..>3439
                     /locus_tag="VFO30_01350"
     CDS             2539..>3439
                     /locus_tag="VFO30_01350"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET9417960.1"
                     /translation="MGGDFREIDKRGMDEIISYHNVVTATPSISRAETVLAQIRLPRQ
                     TRTSMKTAVLVSAAALWCVPCLAKAQSAGERLRAIYTEEWKWRLEQFPGLEGVEKPVP
                     DRLPRVDPATQEMRLRYWQDVLHKLDDVPRAQLSPEEQINSDVYRPEIENFVADQKFR
                     DYEMPANSDSAFWSDLGYTARRPFKTLTDYKNWIAQMRDIPRYFREQIANMRAGLARG
                     FTPPRLTLQGREKSIAAIAEGKPEDNLLYTPFREPMVGVQPSDQDKLKSEAAKVIREI
                     VQPAYADLLKFFRDEYVPHTRTTI"
BASE COUNT          761 a         1017 c          966 g          695 t
ORIGIN      
        1 gctttcgaag attgccgagc tcaaggtgat ctcgcgcacc tccacacaga aatacaaaag
       61 cacgcccgag aacttgcgtg agatcggcca acaattaggc gtagcgcatt ttctcgaggg
      121 cagtgtgcaa aaagtcgcta acgctgtgca cgtgaacgtg cagctgattc gcgccgcaac
      181 ggccgaacat ttatgggcgg aaagctacaa tcgcaaactc gacgatgtct ttggagttga
      241 aggcgaggta gccagcgcca ttgccgagca gctcaacgcc aagctcactg gcgctgagca
      301 aaaagccgtt gcggacacgc cgacccagaa tgcagccgcc tatgacgttt acttacgcgc
      361 ggtcgcaatc gataacgcac tcaccctcga cacgacaaag cgagtcgctg acctttatgc
      421 cgaggcggtt cggcttgatc ctcaattcgc gctggcatgg gctcgcctcg ctgtcgcgcg
      481 aagccagctc tatttcaatg gccttgattt ggacaagaac agcggtgccg cggtgaagga
      541 cgccgcggac cgtgcgatgt cgctccagcc cgagctcggg gaagcatggc tagcgcaggg
      601 cgtttatcgc taccgggtcc tgcgcgattt ccaaggcgcg ctccggtcgt acgaagaggc
      661 gctcaggcgt ttgccaaaca gtgcattcgt gcttcagcag atggctcacc ttgagcgacg
      721 gctcgggcag gctgacgtcg cgcaaaaaca ttatcaggca gccgcacaac tggatccgcg
      781 caacatcggc atccttttaa ccttggccga cacattccgg agcgtccgtc gttatgacga
      841 agcacgggcg gtccttgatc gcgccctgga gatggcaccc ggaaacgagg ccgcactcgg
      901 ggtgaaggca ttgaccttcc aagatgaggg tcgactcaac gaagccgccg agattctggc
      961 gaaagctcct gcgaattccc aggacgaggc ccttatcttc gcgcgagtgg cccagttcta
     1021 cgacgagagg cgcttcgacg aagctatcgc ccagattcag cagaacacgc ccgcggcatt
     1081 ggccaacgat ccgcgcaccc tgacgctgct tggttactgc cagaaatttg ccggtcatag
     1141 cgatgaggcg cgtgctacct ttacgcgcgc agccgcggcg atgaagccaa cacctgactc
     1201 agttgttgtc atcgatgcgc gaaatctgcc gtgctatctc gcgtgggttt atgccggcct
     1261 tggcgaaaag gagaaggcgc tggagcaggc gcgccatgcc atcgctgatt actacagtga
     1321 cgctttagtt aaaccttttg cagaaacctc attggcaatc gtcgaggcgc aaactggcga
     1381 tatcgattca gcgatcgctt ccttgccgca tctgctcgaa atgcccaatg gcgaaacctg
     1441 cagcgatctt cagttaaatc cgctctggga cccgttgcgc aaagacccgc gtttccaaaa
     1501 gctctgctcc ggggcactga gcgaaaactg acaacaatgc gctattttgg cgcggggaac
     1561 atcaccagat tcatgtcgcc caggcgacgt gcgcatatga tttttgttcc atccggcgac
     1621 cagcgcggat tcacaaactc gcccgcagcg tcggtgagtt gcatcgcgcc gctgccgtca
     1681 cgattcattg taaaaacctg gaaccgatca ttcacccgcc gcgagaacac cacgcgtttt
     1741 ccgtcgttcg accaggccgg ccatccatcg agcgtgttct tgccggaaat gttgtgatcg
     1801 tttgtcgcgt ctgcgttcat cagaaagatt tgcgacttcc cgccgtcgac gcgtcggtgc
     1861 aagatcgatg acccatccgg cgagaacgac gcgtacgtgt acccaccgcc agttgttagg
     1921 cgtttcaact ccgtgccgtc agtgcggatg gttgccaggt ccattttctt gtcgatctgt
     1981 tcgccgatta ctttgttgtc gctcggcaca tcgcgaccat ccgccgcggt cgcaaccgca
     2041 aatttcgtgg tgttaaacaa gatgcgctga ctatccggcg cccacgttgg atgaatgttc
     2101 tctgcgttgc cgttggtgag ctggcgcaca tttgtgccgt cggcgttcat cacaaaaatc
     2161 tccgacacac ccgcccggat cgacacaaat gcgatcttct ttccgtccgg cgaccacgcc
     2221 ggcgagtcat cttcgtcact gccgaacgtg agctggcgca tgtcgctgcc gtgactcgcg
     2281 ctgtccgcat tcatcgcata caccttcatc ccgccatgcc ggcttgagac gagcgcaatt
     2341 tttcgcgaat ctggcgacca ggatgcctca taatcaacca cgtatgtgaa ggggctgaat
     2401 ctcttgatcg gaaaatcgtc cgcacaggcg ctagcgatga tcgctgaagt gaagatcgac
     2461 aaagacgcac accaaatgcg catcaggcga ctaacgtacg gcgcgactca gtaaaagcaa
     2521 cgtagcgcgc cttgcgcgat gggcggcgac tttcgtgaga tcgacaaacg tggaatggac
     2581 gagatcatta gctaccacaa cgtggtgacg gcgacgccgt caatcagccg ggctgaaacg
     2641 gtgcttgcgc agattcgtct gccgcgccaa acaaggactt ctatgaaaac tgccgttttg
     2701 gttagtgctg cagcgctgtg gtgcgtcccg tgtcttgcca aggcgcaaag cgcaggtgaa
     2761 cgcctgcggg ctatctacac agaagaatgg aaatggcggc tggagcagtt tcccgggctc
     2821 gaaggagtcg aaaaaccggt tcccgatcgc ctgccaaggg tagatccggc gacgcaggag
     2881 atgcggctac gttactggca ggatgtcctg cacaagctcg atgatgttcc gcgcgcgcag
     2941 ctctcgccgg aggagcagat caattccgat gtttatcgac cggaaatcga gaatttcgtc
     3001 gccgatcaga aattccgtga ctacgaaatg ccagccaact cggattctgc tttctggagc
     3061 gatttgggct acacggcgcg acgaccgttc aagacgctga cggattacaa aaactggatc
     3121 gcgcagatgc gcgacatccc gcgctacttc cgcgaacaaa tcgcgaacat gcgggcgggg
     3181 cttgcgcgcg gcttcacgcc gccgcgcctc actttgcagg gacgtgagaa atccatcgcg
     3241 gccatcgccg agggcaaacc tgaagacaat ctgctctaca caccgttccg cgagccgatg
     3301 gtaggagttc aaccatccga ccaggacaaa ctcaaatcgg aagccgcaaa ggtgattcgt
     3361 gaaatcgtgc aaccggctta tgccgacctg ctcaaatttt tccgcgacga atatgtgccg
     3421 cacacccgaa cgacgattc
//
LOCUS       DASTDG010000042         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1204020, whole genome shotgun sequence.
ACCESSION   DASTDG010000042 DASTDG010000000
VERSION     DASTDG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204020"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VFO30_01355"
     CDS             complement(<1..1878)
                     /locus_tag="VFO30_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET9417961.1"
                     /translation="MTETQESRIAASGCDVRFDNLTRQLYATDASIYQIEPAGVAFPR
                     TAREASIVIRTAADEGVSVTPRGAGTSLVGNAIGEGVIVDFSRHNRKIDNLNIDKRSV
                     RVGAGVVLDQLNAFLKPHGFCFGPDVATSSRATIGGMIANNSSGAHVPVYGTTADHVI
                     STEIVLADGRILTVGPAHDSLGDERRKIDNLIRAHIRELEERMPPGLLKRWSGYGLQR
                     FSRAPNNLNEILAGSEGTLAAIFSAELKISPLPRDKGLGLVFFASVDEAMQATVALLD
                     LKPAAIEHIDRPLLDQTKGQLLFQPARDLMELDAKPCEAILIVEFYDDVAERLSILQS
                     RSLGLRTKIVTEPDEMNLVWSVRKAGLNLLTGCVGPTKPVAFIEDAAVRPAQLPAYVR
                     GLESIINPLGLNASYYGHAATGLLHVRPVLDLHSASDLKKFRQIADETSALVRQFKGS
                     LSAEHGVGIARTEYMREQLGDELLVLMREIKRMFDPKNIFNPGKIFADGQHKIDNHLR
                     DNFTRPIELPFEPRLAFAFKDRSFIGNLEQCNGCGGCRKDAPIMCPTYIATGDEIMST
                     RGRANIIRAALQMRVNGHDPLLSAELDAALSNCLSCKGCTPECPSNVNLALLKAEMMY
                     AR"
     gene            complement(1899..>2417)
                     /locus_tag="VFO30_01360"
     CDS             complement(1899..>2417)
                     /locus_tag="VFO30_01360"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417962.1"
                     /translation="VRQIWGIGEKTERKLQELGVTTCGALQRFSRGELVDLFGKFGLE
                     LYDLCRGIDDRPVEPGRPRKSLSTEQTFAVDLTTLEQCEEKLEELFQDLMADLAQKES
                     SREIRKIFVKLKFNDFTRTTAERAGLAPTLPDFRALLAEAFVRTEKPVRLIGVGVRFA
                     EPAPDDTQIPLF"
BASE COUNT          484 a          672 c          750 g          511 t
ORIGIN      
        1 gcgcgcatac atcatttcag ccttgagcag cgccagattc acattcgagg ggcattccgg
       61 cgtgcaacct ttgcaggaga gacaattact tagcgcagcg tcgagctcgg cggataggag
      121 cggatcatgg ccgttcacgc gcatctggag cgcggcgcga atgatgttgg cgcggccgcg
      181 ggtggacatg atttcatcgc cggtggcaat gtaagtcggg cacataatgg gcgcgtcttt
      241 gcggcagccg ccgcagccgt tgcattgctc gagattgccg atgaatgagc ggtccttgaa
      301 cgcaaacgcc agtcgcggct cgaacggcag ctcaatcggc ctggtgaaat tatcgcgcag
      361 gtgattgtcg atcttatgct ggccgtccgc aaagattttg cccggattga aaatgttttt
      421 tggatcaaac atgcgtttga tctcccgcat cagaacgagc aactcgtcac cgagctgttc
      481 gcgcatgtat tcggtgcgcg cgatgcccac gccgtgttcg gccgagagcg agcctttgaa
      541 ttgtctgacg agcgccgagg tttcgtcggc gatctgccga aattttttga gatcactcgc
      601 gctgtgcaaa tcgagcactg gccggacgtg caagagtccg gtcgcggcgt ggccgtagta
      661 actcgcgttg agtccgagcg ggttgatgat tgattccaaa ccgcgcacgt aagccggcaa
      721 ttgcgcggga cgcaccgcgg cgtcctcgat gaaagcgacc ggcttggtcg gtccgacgca
      781 gccggtcaac agattgagac cggctttgcg gacggaccag acgagattca tctcgtccgg
      841 ctctgtgacg attttcgtgc gcaggcctaa cgaacgtgat tgcaagatcg acaatcgctc
      901 agcgacgtca tcataaaact cgacgatcag aatcgcttcg cacggtttcg cgtcgagctc
      961 catcaggtcg cgagctggtt ggaaaaggag ctggccttta gtctgatcga gcagcggacg
     1021 atcgatgtgt tcgatcgcgg cgggtttgag atcgagcagc gcgacggtcg cttgcatcgc
     1081 ttcatcgact gaagcgaaga aaaccagacc gagaccttta tcgcgtggca gcggcgagat
     1141 tttcagttca gcggaaaaga ttgccgcgag cgtgccttcg ctaccggcga gaatttcgtt
     1201 cagattgttc ggcgcgcgcg agaaccgctg gagaccgtaa ccggaccagc gctttaacag
     1261 tcccggtggc attcgctcct cgagttcgcg aatgtgcgcg cgaatcagat tgtcgatctt
     1321 acgccgctca tcgcctaacg agtcgtgcgc cggaccgaca gtcagaattc tgccatcggc
     1381 gagaacgatc tcggtggaaa tgacgtggtc ggcggtggtg ccatagaccg ggacatgcgc
     1441 gccggaagaa ttattcgcga tcatgccgcc gatcgtggcg cgcgagctgg tcgcgacatc
     1501 cgggccaaag cagaagccgt gcggcttcag aaacgcgttg agctgatcga gcacgacacc
     1561 ggcgccgacg cgcacgctgc gcttgtcgat gttgagattg tcgatcttgc gattatggcg
     1621 ggagaaatcg acgattactc cttcgccaat cgcattgccg acgagactcg tccccgcgcc
     1681 acgcggcgtg acggaaacgc cttcgtcggc cgccgttcga atgacgatgc ttgcttcccg
     1741 cgccgtgcgc ggaaacgcca cgcccgcggg ctcgatttgg taaatcgacg cgtcggtcgc
     1801 gtaaagctgc cgtgtgagat tatcgaagcg cacgtcacaa cccgaagcgg cgattctact
     1861 ttcctgtgtc tcagtcatgt agagttggcg aagataactt agaagagggg aatttgcgtg
     1921 tcgtccggcg ccggctcggc gaaccgtacg ccgacgccaa tcaggcgcac aggtttttcg
     1981 gtgcgcacga atgcttcagc gagcagcgcg cgaaaatctg gcagtgtcgg tgcaagtccg
     2041 gcgcgctcgg ccgttgtgcg ggtgaagtcg ttaaacttca gctttacgaa gattttcctg
     2101 atctcgcggc tcgattcttt ctgcgcgaga tcagccatta aatcctggaa caattcctcg
     2161 agcttctctt cgcattgctc gagcgtcgta aggtcgaccg caaatgtttg ctcagtgctg
     2221 agtgatttgc gcggccggcc gggttcgact ggacggtcgt caatgccgcg gcagagatcg
     2281 taaagctcga ggccgaactt gccgaaaaga tcgacaagtt cgccgcggga gaagcgctgc
     2341 agggcgccgc acgtggtgac gccgagttcc tgaagtttgc gctcggtctt ttcaccgatt
     2401 ccccagatct gccggac
//
LOCUS       DASTDG010000043         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_917186, whole genome shotgun sequence.
ACCESSION   DASTDG010000043 DASTDG010000000
VERSION     DASTDG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917186"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2110
                     /locus_tag="VFO30_01365"
     CDS             <1..2110
                     /locus_tag="VFO30_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9417963.1"
                     /translation="ATGGIVSANYFDALGVRPMLGRGFRPEEGTGRNAHPVTVISYLT
                     WQDRYKGDPEIIGKTQYLNGVQHTIIGVAPEKFHGTFIGYSFNFWVPTSMQETFDSTG
                     YKLEDRGARWIESYAFLKPGVTREQAQAELNAIAQRLENDFPDTDRGHGIEILPLWKT
                     PFNAVGNLSPTLAITSAVAFFVLLIACANVSNLLLARSLIRRHEMTMRLALGAGRRRL
                     IKQLFTEGLLLSLIAAAGGIMVAYWCRNALVLISPTRIPGITIDYPGQLDWRVLALSV
                     AVCIGSTMLFALIPAIQASHVDLSGALKSEGSGVVGGSGRSRLRSALVFVQVALSFVL
                     IAGTGLLLRTLVQMQNSDPGFSTRNVVVSAVDLFSAGYKPDQAKIFYEQMLERIRALP
                     GVQSAALARVRPFSYTVYSSAPLEIEGYQPPRNEQVTADYNQVGEGYFATIGIPIVTG
                     REFTRNDDENAPSVAIVNETMAAKYWPGKNAIGQRLKVKDKWMEVVGLAKDSKYQTML
                     EAPKPFFYVPVRQNFFVQNGFIIRTDQSAAAIMNALAREIHALDPNLAPLDTISLQQQ
                     VDRMSYTQRLAATLLAIFGGMALFLAAIGLYAVMSYAVSQGTRELGVRMALGANAGNL
                     LRLVISRGLRLTTAGIVVGAIAALLLTRLIGNLLYKVSPRDPLAFGSALVILTAIALV
                     ACFMPAWRATRIDPVQALRM"
     gene            2154..3479
                     /locus_tag="VFO30_01370"
     CDS             2154..3479
                     /locus_tag="VFO30_01370"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9417964.1"
                     /translation="MTRSAANKDTAIAQPGSFSPVLSAMDVARPDLALKKRKRRVVII
                     SAVAFGVILVTAGLSRLKPGVPILDRTTVWIDTVKRGPMVREVRGLGTLVPEQIRWLA
                     SNVDARVDRIILRPGAKVEPDTIILELNSPELEQAANDAQSKAKALEADLTSLRATLQ
                     RTLLDQESTTAKVHSEYEQAKMERQTNDQLAKNGLVADLIYKTSQVKEAELTKRDEIE
                     QKRLAFLRDSIEPQLASKQAEVDQANQLAKLKSDEVEALQVRAGISGVLQQLPLEVGQ
                     RVTAGTNLARVADPTSLKAQLKIPETQAKDIQLDEQAVIDTRNGTVKGHVTRVDSAVE
                     QGTVTVDVAFDETLPKGARPDLSVDGIVELERLDNVTYVGRPTFGQENTTVGIFKLVT
                     GSNDAVRTSVKLGRSSATAIEVLSGLKPGDQVILSDTSAWSESQRVRLK"
     gene            3500..4198
                     /locus_tag="VFO30_01375"
     CDS             3500..4198
                     /locus_tag="VFO30_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9417965.1"
                     /translation="MNNGRIPLIQLENLTKVFLTDEVETRALSGVNLEIHNGEYVSIA
                     GPSGCGKSTLLSILGLLDTPSSGSYTLKETEIGNLSFADRARIRNREIGFIFQSFNLI
                     GDLSVYENVELPLNYRGLSSRERRRLANEALERVGMRHRANHLPSQLSGGQQQRVAVA
                     RALAGQPAILLADEPTGNLDSRNGEAVMDLLKQLHASGATICMVTHDERFAEYADRTV
                     HLFDGHIVEEPIAA"
BASE COUNT          991 a         1296 c         1279 g          954 t
ORIGIN      
        1 cgcgacggga ggaattgttt ccgcaaatta ttttgatgcg ctgggtgttc gcccgatgct
       61 tggccgcggc tttaggccgg aagaaggcac ggggcgcaat gcacatccag tcaccgtaat
      121 tagttacctg acctggcagg accgttacaa gggcgacccg gagatcatcg gcaaaacgca
      181 atatctcaac ggcgttcaac acacgatcat tggcgtcgcg ccggaaaaat tccacggcac
      241 cttcatcgga tactcgttca acttctgggt gccgacgtcg atgcaggaaa cgttcgattc
      301 gaccggctac aaactcgaag accgcggggc gcgctggatt gaaagttacg cgtttttgaa
      361 accgggcgtg acgcgtgagc aggcccaagc cgagttgaac gccattgcgc aacgtctgga
      421 aaatgatttc cccgacactg atcgcggtca cggcattgaa attctgccgc tctggaaaac
      481 gccattcaac gccgttggaa atctgtcgcc cacgctagcg atcacaagcg cggtcgcgtt
      541 tttcgttctc ttaatcgcct gcgcgaacgt gagcaatctt ctgctcgcgc gctcactcat
      601 tcgccgtcat gagatgacga tgcgtctcgc gctcggtgcc gggcgccggc gtttgattaa
      661 acaacttttc actgaagggc tgcttctttc tctaatcgca gccgccggtg gaatcatggt
      721 cgcgtactgg tgtcgtaacg cccttgtgct gatatctcct acccgcattc cgggcatcac
      781 aattgattat cccggacaac tggactggcg tgttctggct ctaagcgtgg cggtttgcat
      841 cggatcgaca atgctgttcg cgttgatacc tgcgattcaa gcgagtcatg tcgatctttc
      901 cggcgcgctc aagagtgaag gcagcggagt agtaggcggc agcggtcgat cgcgactgcg
      961 ctcggcgctt gtgttcgttc aagtagcgct gagttttgtg ctcattgccg gaactggatt
     1021 actgctccga accctggtgc aaatgcaaaa ttccgatccg ggattttcga cacgaaacgt
     1081 cgtcgtgtcg gcggtagatc ttttttctgc cggctacaaa cccgatcagg ccaaaatttt
     1141 ttacgaacaa atgctcgagc gcattcgggc actgcccggc gttcaatcgg cagcattggc
     1201 gcgggtcagg cctttcagct acacggttta ctcgtcagcg ccgctggaga tcgaaggtta
     1261 tcagccgccg cgcaatgagc aggtgacggc ggattacaat caagttggcg aaggatactt
     1321 tgccaccatt gggatcccca ttgttacagg tcgcgaattc acccgcaacg atgacgaaaa
     1381 cgcaccatcg gttgcgatcg tcaatgagac gatggcggca aaatattggc cggggaaaaa
     1441 cgcgatcggg caacgactca aagtcaaaga caagtggatg gaggtggtcg gtctcgcgaa
     1501 agattccaaa tatcaaacga tgctcgaggc gccgaagcca tttttttatg tgccggttcg
     1561 ccagaatttt ttcgtccaaa acggtttcat cattcggacg gaccaaagcg cggccgcgat
     1621 catgaacgcg ctggcccgcg aaatccacgc gctcgatccg aatctcgcgc ccttggacac
     1681 aatcagtttg cagcagcaag tcgatcgcat gagttacacg caacggctgg cggcgacgct
     1741 gctggcgatt tttggtggaa tggcgctctt cctcgccgcg atcggacttt atgccgtcat
     1801 gtcctacgcg gtttcgcaag gcacgcgcga gctcggtgtg cgcatggcgc tcggcgcgaa
     1861 cgccggcaat cttttgcggt tggttatttc gcgcggtttg cgtctaacga cggccggaat
     1921 tgttgtcggg gcgattgccg cgctcttgct gactcgcctg atcgggaacc ttctttacaa
     1981 agtcagcccg cgcgaccccc tcgcgtttgg gtcggcgctc gtgatcctga cagcgattgc
     2041 attggtcgcc tgttttatgc cggcctggcg cgcaacccga atcgatccgg tccaggcttt
     2101 gcggatgtga tgactctgag aagagcaaac aatcgccacg cgctgatccg aaaatgacaa
     2161 ggagcgcagc aaacaaagat acggcaatcg cgcagcccgg cagcttcagc ccggtcctct
     2221 ctgccatgga cgtcgcccgg cccgatctgg ccctgaaaaa gcggaaacgc cgtgtcgtca
     2281 tcatttccgc tgtcgcgttc ggcgtcatct tagtgaccgc cggcctgtcc cgcctcaagc
     2341 ctggagttcc aatcttggat cgtacgacgg tttggatcga cacggtcaaa cgcggtccga
     2401 tggtgcgtga agtacgcggg ctgggcacgt tggttccgga acagattcga tggctcgcca
     2461 gtaacgtcga tgcgcgcgtt gatcgaatta tcttgcggcc gggcgcgaaa gtggaacccg
     2521 acacgatcat tttggaattg aacagccctg agctcgagca agctgcgaac gacgcgcaat
     2581 cgaaggccaa agccctcgaa gccgatctga ccagtttgcg cgcgacattg cagcgaacgc
     2641 tcctggatca ggaatccacc accgcgaaag ttcattctga gtacgagcaa gccaaaatgg
     2701 agcggcaaac caatgaccaa ttggcaaaaa acggattggt ggcagacctc atttacaaaa
     2761 cctcgcaagt taaagaggcg gagctcacga agcgcgacga gatcgagcag aagcggttgg
     2821 cctttttgcg cgattcgatc gaaccgcagc tcgcgtcaaa acaggcagag gtcgatcaag
     2881 ccaatcagct ggcaaagttg aaatccgacg aggtcgaagc gttgcaggta cgcgcgggta
     2941 tctcaggcgt gctacaacaa ttgccgctcg aagtcgggca acgcgtcacc gccggcacga
     3001 acctcgcccg ggtggctgat ccgaccagtt tgaaagcgca acttaaaatt cccgagacac
     3061 aggcgaaaga catccagctc gatgagcaag cggtgatcga cacgcgtaac ggaactgtca
     3121 aaggccatgt cacccgcgtc gattcggccg tcgagcaagg aacggtgact gttgatgttg
     3181 cctttgacga gacattgccc aaaggcgccc ggccggattt aagtgtggac ggaatcgtcg
     3241 agctggagcg tctcgacaac gtgacttatg tcgggcggcc gacatttggt caggaaaaca
     3301 cgaccgttgg aattttcaaa ctcgtcaccg gcagcaacga cgcagtgcga acctccgtca
     3361 aattgggcag aagttcggcc accgcgattg aagttctcag cgggctgaag cccggcgacc
     3421 aagtcattct ctccgacacg agcgcctggt cggagtccca gcgagttcgc ctcaagtaaa
     3481 taaatcagcc atcagaaaca tgaacaacgg acgaattcct ctcattcaac tcgagaacct
     3541 cactaaggtt ttcctcacag acgaagtgga gacgcgcgcg ctttccggag tcaatctcga
     3601 aattcataac ggtgagtacg tttcgattgc gggcccgtcg ggctgcggca aatcaacctt
     3661 attgtcgatc ctcggtcttc tcgacacccc atcgagtgga agctacacgc tgaaagaaac
     3721 cgaaatagga aatctttcct tcgcggatcg cgctcgcatc cgcaatcgtg agattggctt
     3781 catcttccag agcttcaatt tgattggcga tctgagcgtg tacgagaacg tcgagctgcc
     3841 actgaattat cgcgggctca gttcgcgcga gcgcagacga cttgcgaacg aagcgctcga
     3901 gcgcgttggc atgagacatc gcgccaatca tctgccgagt cagctctccg gcggccagca
     3961 acaacgcgtc gcggtggccc gggcgctggc cggtcaaccg gcgatcctgc tcgcggacga
     4021 accgaccggc aacctcgatt cgcgcaatgg cgaagcggtc atggatttgc tcaagcaact
     4081 ccatgccagc ggcgcaacca tttgcatggt cacacacgac gagcgcttcg ccgaatacgc
     4141 cgatcgtacc gttcacttgt tcgacggcca catcgtcgag gaaccgatcg cagcttgagt
     4201 ccgagctcgc cacaggacga gcccgcccga ttggccgacc ggactgatgg cctcgcgttg
     4261 tcgcgccgca gcggtcccct gatcaagttc tcaactgctt aacttcggtt gacgtcgatc
     4321 ttggtggttt cgaccacttt ctcgaagcca cgctaccggg cacgtatttg ccgctttaca
     4381 ccatggtcac gttcacacgc ctgccctacg ccgaagcgac tcggcggacg cgccgtcaag
     4441 acgcgttatg tacacgctgc ttattgcggt tgcggcggta acgctcttcg gtctggctgt
     4501 tttcatcaat ggcctccatt
//
LOCUS       DASTDG010000044         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1319677, whole genome shotgun sequence.
ACCESSION   DASTDG010000044 DASTDG010000000
VERSION     DASTDG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319677"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1184)
                     /locus_tag="VFO30_01380"
     CDS             complement(129..1184)
                     /locus_tag="VFO30_01380"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET9417966.1"
                     /translation="MPAAAQNYSLGLQVEVAKIDNELKKLWQEGAGAMTRASLINLAV
                     YSEEPGSLEKNTQLVAKITEDHACRALVIFADHSAQEDRVDAWISAHCHVTRAGSTQV
                     CSEQLSFWLQGHCAKLLPSIVFSHVDSDLPLYLWWQGEFHDPMDPQLWAWVDRLLYDS
                     HSWKNFADQLRLIETAQREARQRIVLCDLNWMRLDKIRFAVAQFFDHPASHHHFGKIR
                     SAQIQFTPGFKSTAVLFAGWLGAQLNWRVDSANGAAFQFADANGRKIDIELRENDGPP
                     IGKIVINSTEAEFSVAPAQCGDLLEISRSRPNEQRAGQLMPAQCYDLADLMTQELLRG
                     GPHHIYLRAINCVRDLL"
     gene            complement(1186..2745)
                     /gene="zwf"
                     /locus_tag="VFO30_01385"
     CDS             complement(1186..2745)
                     /gene="zwf"
                     /locus_tag="VFO30_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9417967.1"
                     /translation="MNFAMDRAAEQEQANPLREGLSMRAVPQPCSVVIFGATGDLTHR
                     KLIPALYNLAADGELPPATAVIGFARREKSDDEFRKELEETTRNFSRQPLRDDIWNTF
                     AQSIFYHQCEFEDEAGYKSLSQRLDKIDKESGTRGNRLFYLAAAPDQFEAILKNLKGA
                     GLNQTCAGSWVRVIVEKPFGSDLDSARELNRIVRNSFSEEQTYRIDHFLGKETAQNIL
                     VLRFANAIFGPLWNRHYVDHIQITAAETLGVEGRAGYYETAGALRDMVQNHLLQLLCL
                     VAMEPPTDLEANSIRDEKVKVVRSLRRYSRAEIAANVVRGQYAEGAINGKPVAGYRQE
                     KNVNPKSMIDTFVALRLYIDNWRWSGVPVYMRVGKRLPKSGTEISVHFKRAPAVLFNR
                     EVDALDQNVLVIRIQPDEGISLRMQAKMPGTHFRIQPVKMDFHYGTSFGKASPEAYER
                     LLLDAMSGDATLFARRDEVEEAWSFIDRIEDAWAAKQNAPALFFYPAGSWGPEEADEL
                     LARDDRSWRRF"
     gene            complement(3101..>3506)
                     /locus_tag="VFO30_01390"
     CDS             complement(3101..>3506)
                     /locus_tag="VFO30_01390"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980932.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9417968.1"
                     /translation="FREYQRQVVVNAKALAAGLTKHGYRIVSGGTDNHLMLVDLRPKE
                     LNGTEAQEVLDRAGITVNKNAIPFDTYPVFKPGGIRVGTPAATTRGMKEEEMLEIADL
                     IAEALEKRIDADALNRVREKVRELTRRFPIPA"
BASE COUNT          660 a         1035 c         1023 g          788 t
ORIGIN      
        1 tgaaaagatc gcagcagcgt tgattcgtct cttcgagcaa ttgcgtttgt agccgcgggc
       61 ctgtagcccg tctgggctga cggcgcaccc gccttcgccc tgcttcggcg tggcaagcag
      121 cgcagtggct acagaagatc gcgcacgcaa ttgatggcgc gcagataaat gtgatgcggt
      181 cctccgcgca ataactcttg cgtcatcagg tcggcgagat cgtagcattg tgcgggcatg
      241 agctggccgg cgcgctgttc gttaggtcgg ctgcgtgaga tttccagtag gtcgccgcat
      301 tgcgcgggcg cgacgctgaa ttcggcttcg gtcgagttga ttacgatttt gccaatcggc
      361 ggtccgtcat tctcacgcaa ttcgatgtcg atcttgcgac cgttcgcgtc ggcgaattgg
      421 aacgccgcgc cattggccga atcaacccgc caattcagtt gagcgccgag ccagccggcg
      481 aagagaacgg ccgtggactt gaaccccggg gtgaactgga tctgcgcgct tcgaattttg
      541 ccgaaatgat ggtgtgacgc ggggtggtcg aagaattgag cgacagcgaa acggatttta
      601 tccagccgca tccagttcaa atcgcaaagc acgatacgct ggcgagcttc gcgttgcgcc
      661 gtttcgatga gccggagctg atcggcaaaa ttcttccagg agtggctgtc gtaaaggagg
      721 cggtcgaccc acgcccaaag ctgcggatcc atcgggtcgt gaaactcgcc ctgccaccac
      781 aggtaaagcg ggaggtcgga atcgacgtgc gagaagacga tgctcggcag cagtttcgcg
      841 cagtgtccct gcaaccaaaa agataactgc tccgaacaca cttgtgtact cccggcacgc
      901 gtgacatgac aatgggcgct gatccacgca tcaacccgat cctcctgcgc tgaatgatca
      961 gcgaaaatga cgagcgcgcg acaggcgtga tcctcggtga ttttcgcgac gagctgcgtg
     1021 ttcttttcga gcgagcctgg ttcctcgctg taaacggcga gattaatgag cgaagctcgc
     1081 gtcatcgcgc cagcgccttc ctgccagagc tttttcagct cgttgtcgat cttagcgacc
     1141 tcgacctgca ggcctaacga atagttctgt gccgccgcag gcatattaga atctcctcca
     1201 cgagcggtcg tcgcgcgcca gcagctcgtc agcttcttcc ggtccccacg agccggctgg
     1261 atagaaaaac aatgccggtg cgttttgttt tgccgcccag gcgtcctcaa ttctgtcgat
     1321 gaacgaccac gcctcctcga cctcatcgcg gcgcgcgaac aatgtggcgt ccccgctcat
     1381 cgcgtcgagc agcagccgct cgtacgcttc gggactcgct ttgccaaacg aggtcccgta
     1441 atggaaatcc atctttaccg gttgaatccg gaaatgcgtg cccggcattt tcgcctgcat
     1501 ccgcagggaa attccttcat ccggctgaat gcgaatgacg aggacgtttt ggtcgagcgc
     1561 gtcgacttct cggttgaaca aaaccgccgg ggcccttttg aagtgcaccg agatctcggt
     1621 gccggatttt ggcaggcgct ttccgacgcg catgtaaact ggaacgccag accagcgcca
     1681 gttgtcgatg tacaaccgca acgcgacgaa cgtgtcgatc atcgacttcg gattgacgtt
     1741 cttctcctga cgataaccgg cgaccggttt tccattgatt gcgccttccg cgtactggcc
     1801 ccgtaccacg ttggctgcga tttcagcgcg cgagtatcgg cgcagcgacc ggacgacctt
     1861 caccttttcg tcgcggatgc tgtttgcttc gagatccgtt ggcggctcca tcgccacgag
     1921 gcacagtaac tggagcaggt ggttctggac catgtcgcgc aatgctccgg cggtctcgta
     1981 ataaccggca cgaccttcga caccgagcgt ttccgcggca gtgatctgaa tatgatcgac
     2041 gtaatgccgg ttccagagcg ggccgaaaat cgcgttggcg aagcgcagca cgagaatgtt
     2101 ttgcgccgtc tcttttccga ggaaatgatc gatccggtag gtttgttctt cgctgaagga
     2161 attgcgcaca atccggttca gctcgcgcgc tgaatccaaa tcgctgccga agggtttctc
     2221 gacgatcaca cgtacccaac tgccggcaca cgtctggttg agaccggcgc ccttcagatt
     2281 tttcaggatc gcctcaaact ggtccggcgc cgctgcaagg taaaagagcc gattgccgcg
     2341 tgtgccgctt tccttgtcga tcttgtccag ccgctgcgac aggcttttgt agcccgcctc
     2401 gtcttcgaac tcgcattgat gatagaaaat tgattgcgcg aaggtgttcc agatgtcatc
     2461 gcgcagcggt tgccgcgaaa agttgcgcgt agtttcttcg agttctttgc gaaactcatc
     2521 gtcgctcttt tcccgccggg cgaagccgat aacggccgtg gccggcggca gttcgccatc
     2581 cgccgctaaa ttgtaaagcg ccgggatcag tttgcgatgc gtcaggtcgc cggtcgcgcc
     2641 gaagatcacg acggagcacg gctgcggcac cgcgcgcatc gacaaacctt cgcgcaatgg
     2701 gttcgcctgc tcttgctcgg ccgctcggtc catcgcgaag ttcaccttac gcaaattctc
     2761 ggcgattagg taagagcaat ttatcgcggg cacactctgc caaccggcgc gcaaccgctc
     2821 actgcctggt ggcgccatgc ttgttcagga gcgagcaaat gcgcagcaac ggcgcctctt
     2881 cgctcatcgt ttaatttgcc gagctacttc gactaaccgc ctgttttccc acctctctaa
     2941 atcttcccat gtgcgcgctt taaagacgaa accgcggcgg cgttttacct ggagcgcttt
     3001 cgcctcggcg tccaatcgcg ccccgagcgc gtaaaattct tgcggtgtct ttggaggcgg
     3061 gcgtttgcgg acaatcttga tcttcattag tcgttgctct tcacgccgga atcgggaatc
     3121 gacgcgtgag ctcccgcact ttttcgcgca cgcggttgag cgcatcggcg tcgatgcgct
     3181 tttcgagcgc ctcggcgatg agatcggcga tctcgagcat ctcttcttct ttcatgccgc
     3241 gcgttgtggc ggcgggtgtg ccgacgcgaa tgccgccggg cttgaacacc ggataggtgt
     3301 cgaacgggat cgcgtttttg ttcacggtaa tgccggcgcg gtcgagcact tcctgcgctt
     3361 ctgtgccgtt gagctctttc ggtcgaaggt cgacgagcat gaggtgattg tcggtgccac
     3421 cggagacaat gcgataaccg tgtttcgtta ggccggcggc gagcgccttc gcgttcacga
     3481 caacctgacg ctggtactcg cgaaac
//
LOCUS       DASTDG010000045         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1285830, whole genome shotgun sequence.
ACCESSION   DASTDG010000045 DASTDG010000000
VERSION     DASTDG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285830"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..457)
                     /locus_tag="VFO30_01395"
     CDS             complement(173..457)
                     /locus_tag="VFO30_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417969.1"
                     /translation="MTPSELLSFFLEGWGLPFTIYVLAQIFVPIRLRGAWRGLSLLPI
                     PAMAYVVYATVGAFKEHSNMWPILLIIASPVAAFFLVLLFCLTRNWRDAV"
     gene            complement(534..1637)
                     /locus_tag="VFO30_01400"
     CDS             complement(534..1637)
                     /locus_tag="VFO30_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9417970.1"
                     /translation="MRKIRIAIVGVGNCASSLIQGINFYRSSGGRGNGVGLMHRQIGP
                     YRPDDIEVVAAFDIDRRKVGIDVGRAIFAPPNCTKVFCEKIALTGAIVKMGVVLDSYA
                     EHMRDQDPLRTFLPLEKESTREQIVAELKDSAAEIMVNYLPVGSEEATRFYASCALEA
                     GIALVNNIPVFIASDPVWAKKFADKNLPIVGDDIKAQLGATVLHRMIVDLFRKRGVKV
                     ERTYQLNTGGNTDFLNMLNRTRLASKKTSKTEAVQSVIGERLADENIHIGPSDYVPWQ
                     LDNKVAFIRVEGRLFGDVPMEVEVRLSVEDSPNSAGIAIDAIRCCKLALDRGIGGVLH
                     SASAFFSKHPPVQMTDDEAYRCVEQFIRGERDR"
     gene            1797..>2765
                     /locus_tag="VFO30_01405"
     CDS             1797..>2765
                     /locus_tag="VFO30_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9417971.1"
                     /translation="MRLPFRAVALSSLLVLAGTSRAAPPSYNLEQAVALAQEKNPEIA
                     IARKKVEAARGEVIEARSGFLPSVSSVGLLDKRQHERETRLRDEDYNAQLRLQQNLYT
                     GGAITSQLAIARLNLDKAQCDLQETINRVAMDTRVAYSELLLNRARVRVREDSVRVLE
                     QELKNQQERLRAGLVATLNVQRAEVTVANERPELINAQTDLQMSYLRLGEIFGMEWPL
                     DGAPAPFDVAGQLQYQARHPVLNDCLARADAERPSIRAAQRAIDIEDRQYVLDKSEVR
                     PHVGFFSGYEVYNERDPEVGQEFNYGYVFGINGTWHIFDGFATKGRL"
BASE COUNT          605 a          710 c          898 g          552 t
ORIGIN      
        1 ataggtattg tatttcgttg gtttggttgg aacgcaaatt gagttgttgc agtggcctaa
       61 cgagaaggag ctgagccacc gctcgcggcg gcgagcgtgg caagcatcaa gaactgtttc
      121 atagaatcaa atgtggactt caaagcggcc agcggttggc tccagcgatt ggttagactg
      181 catcgcgcca attacgagtg aggcagaaga gaaggacgag aaaaaatgct gccaccggac
      241 tggcgatgat gagaagaatt ggccacatgt ttgagtgttc cttgaatgca ccaacggttg
      301 cataaacgac gtaagccatg gccggaatcg ggagcaggga gagcccgcgc caagcaccac
      361 gcagccggat ggggacgaag atttgcgcaa ggacataaat agtgaatggc aagccccaac
      421 cctcgaggaa gaacgagagc aactctgagg gagtcattgc ggtctaacga gaattagacg
      481 aagattttcg tctattcgat tgcgggggag ccgcatgatc cccttttgtt agattagcga
      541 tcgcgctcgc cgcggatgaa ttgctcgacg cagcggtagg cttcgtcgtc ggtcatttgc
      601 acgggcggat gcttggagaa gaaggcgctg gcggaatgga ggacaccgcc gatgccgcgg
      661 tcgagcgcga gcttgcagca gcggatggca tcgatggcga tgccggcgga gttgggtgag
      721 tcttcgacgg aaaggcggac ttcaacttcc atcggcacgt cgccgaagag gcggccttcg
      781 acgcggatga atgcgacttt gttgtcgagc tgccagggaa cgtaatcgct cgggccgatg
      841 tggatgtttt cgtcggcgag acgttcgccg atcacggatt gcacggcttc ggtcttggaa
      901 gttttcttcg aggcgagccg ggtccggttg agcatgttga ggaaatcggt gttgccgccg
      961 gtgttcaatt gataggtgcg ctcgactttg acgccgcgtt tgcggaaaag atcgacaatc
     1021 atgcgatgaa gcacggtggc gccgagctgg gctttgatgt cgtcgccaac gatggggaga
     1081 tttttgtcgg cgaacttttt cgcccagacc gggtcgctgg cgatgaagac ggggatgtta
     1141 ttgactaaag cgatgccggc ctcgagcgcg caactggcgt aaaagcgagt cgcttcctcc
     1201 gagccgaccg ggagataatt gaccatgatc tcggcggccg aatccttcag ctcggcgact
     1261 atttgttcgc gggtcgattc tttttcgagc ggcaggaacg tgcgcaacgg gtcctggtcg
     1321 cgcatgtgct cggcgtaact gtcgaggacg acacccatct tcacgatcgc gccggtgagg
     1381 gcgatttttt cgcagaagac cttggtgcaa ttgggggggg cgaagatggc ccgtccgaca
     1441 tcgatgccga ctttgcggcg gtcgatgtcg aaggcggcga cgacttcgat gtcgtcgggc
     1501 cgataggggc cgatctggcg atgcatcagt ccgactccgt tgccgcggcc gccggagctg
     1561 cgatagaaat taatgccttg gatgagggag ctggcgcagt tgcccacgcc aacgatagcg
     1621 atgcgtattt ttctcattcg aagagtggtt aggctagccg gagcaatgcg ctcgggcaag
     1681 gtgcaactgg cggccattct tccatagtca cggcggcttg caagggctga cataactata
     1741 aaaaccagag ggatttttgt tgaaaaacag cgccgctggt caacagatag aaccccgtgc
     1801 gtctgccgtt cagagccgtt gcgttgtcga gcttgctcgt gttggcgggg acgagccgtg
     1861 ccgcccctcc gagctacaac ctcgaacagg cggtcgcact cgcgcaggag aaaaatcctg
     1921 agatcgcgat tgcgcgcaaa aaagtggaag cggcgcgcgg tgaagtgatc gaggcgcggt
     1981 cgggattttt accgtcggtc agctcggtcg gcctgctcga caagcggcag catgaacggg
     2041 agacacggct gcgcgacgaa gattacaatg cgcagctgcg gctgcagcag aacctctaca
     2101 cgggcggcgc aattacgagc cagctcgcga tcgcgcggct gaatctcgac aaggcccaat
     2161 gcgatttaca ggaaaccatc aatcgcgtcg ccatggacac gcgcgtcgcg tattcagaac
     2221 tgcttctcaa ccgcgccagg gtgcgagtgc gcgaggattc ggtgcgagtg cttgagcagg
     2281 agttgaaaaa ccagcaggaa cgcttgcgcg ctggattggt cgccacgttg aacgtgcagc
     2341 gcgcggaagt gaccgtggca aacgagcgtc cggaactgat taacgcgcaa acagacctac
     2401 agatgtcata ccttcgcctc ggcgaaatat tcggaatgga atggccgctc gatggtgcac
     2461 cggcaccatt cgatgtcgct gggcaactcc agtaccaagc gcgccatccc gttttaaacg
     2521 actgccttgc acgcgcggac gcggaacgcc cgagcattcg cgcggcgcaa cgcgcgatcg
     2581 acatcgaaga tcgacaatat gtcttggata agagcgaggt ccgcccgcac gtcgggtttt
     2641 tctcgggcta cgaagtttac aacgagcgcg acccggaggt cggacaagag ttcaactacg
     2701 ggtacgtgtt cggcatcaac ggcacgtggc atatttttga tggcttcgcg accaaaggcc
     2761 ggctc
//
LOCUS       DASTDG010000046        22681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_380517, whole genome shotgun sequence.
ACCESSION   DASTDG010000046 DASTDG010000000
VERSION     DASTDG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 22681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22681
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380517"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFO30_01410"
     CDS             complement(<1..829)
                     /locus_tag="VFO30_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9417972.1"
                     /translation="MTSAQIRQSFLDFFREKQHTIVPSSSLLPDAPNLLFTNAGMNQF
                     VPIFLGQQKPPWSPARVADTQKCIRAGGKHNDLEDVGLDTYHHTFFEMLGNWSFGDYF
                     KKEAVEWAWELVVEGWKFPAQRLYATVYKPGPDEPAEFDQEAHDHWARLFADADLDPK
                     VHVLTGGKADNFWMMGDTGPCGPCSELHVDLTVDGDTRGVLVNKNDPRCFEIWNLVFI
                     QFNANPDGTFTPLPQRHVDTGMGFERVTAIIQGTKNLSDFSGAISNYETDIFRPIFNE
                     "
     gene            complement(971..2488)
                     /locus_tag="VFO30_01415"
     CDS             complement(971..2488)
                     /locus_tag="VFO30_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417973.1"
                     /translation="MRGLGDLHHPVSTKNREAQQFFDQGLKLVYAFNHDEARRSFERA
                     LQLDPQLGMAWWGVALTLGPNYNLPVDPAREKAAYEAVQHALALQDNASDPERAYINA
                     LAARYSNDPHVDLHALDIAYKDAMAKLAARYPDDLDAATLFAESMMNLNPWHLWTADG
                     RPAEGTDRIVWVLESVLKRDPNHVGANHYYIHAIEASPNPERALASAMRLEKLANAAG
                     HLVHMPAHIYARVGDHAAAAHSNKLAVAADQKFIRETHEQGVYPLMYYSHNLHFLAYA
                     DCMRGDFAEAKTAAAQLVANVAPGVKSMPELEGFLPTPILILLAFERWSDILELPKPD
                     PSVAVSNAVWHSVRGIALANVGRSAEAENEQKSFRDLSAKIPADKMYDPLNQAAAVLK
                     VHESLLAAAIAGSHRDANATVEFLKGAVAAEDALNYSEPPTWYPPVRPLLGRVLLANK
                     RVADAETVFRADLDRNPRDARALAGLRDCLNAQGRSYEAAQIDREFRAAWKLPQS"
     gene            complement(2610..3653)
                     /gene="recA"
                     /locus_tag="VFO30_01420"
     CDS             complement(2610..3653)
                     /gene="recA"
                     /locus_tag="VFO30_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9417974.1"
                     /translation="MATKTATAEKTGEDKLTVARNKNLDLAIQQIAKDYGEGAIMRLG
                     DEKIVDIDVIPTGNILIDRALGVGGFPRGRVVEVFGPESSGKTTLTLTVIAQAQKRGG
                     LAAFIDVEHALDPQYARKLGVSLDDLLVSQPSSGEEALRICETLIRSNSLDVIVVDSV
                     AALVTRAELEGEIGDATVGAQARLMSAALRKLTSLISKARTCCIFTNQIREKIGVMFG
                     NPETTPGGKALKFYASMRVDIRRIGAIKQTDGTVTGNRTRIKVVKNKVAPPFTEAEFD
                     IMYNEGISLTGALLDLALEKQIIEKRGSWLIYKGTQLAQGRDAAKDVLKNDRALYEEI
                     ESGVKAKLDEEKT"
     gene            complement(3787..4164)
                     /locus_tag="VFO30_01425"
     CDS             complement(3787..4164)
                     /locus_tag="VFO30_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9417975.1"
                     /translation="MNTTVRDNREIEARGAQAEQFVAPTASVVEENDGYTLRIEMPGV
                     TKEGLEISMENNELSIIGRRSLPTIDGTLIHYELQRENFRRVFELDPSIDANRISANM
                     DQGILTLTLPKAEHLKPRKITVS"
     gene            complement(4201..4590)
                     /locus_tag="VFO30_01430"
     CDS             complement(4201..4590)
                     /locus_tag="VFO30_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9417976.1"
                     /translation="MNAFLELPFWSTFGRQGQLLSGWAPALDLYQNNDTVVAVVELPG
                     MRKEEIEISLHNGTLTIAGERKAEAGNGDKAERTERYVGKFRRSISLPVRVDANKVSA
                     TYRDGILIVTLPKADEAKPKQIKVRAD"
     gene            4953..5996
                     /gene="ribD"
                     /locus_tag="VFO30_01435"
     CDS             4953..5996
                     /gene="ribD"
                     /locus_tag="VFO30_01435"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9417977.1"
                     /translation="MNFADADERFMRVALNEARKSLGRTSPNPAVGAILVCGGRILAR
                     AHHTRAGEPHAEIACLHKLGGPVPADATLYVTLEPCSTTGKTPPCTRAIIDAGVRTVV
                     IGAVDVNPRHSGRGISELRNAGVMLRTGVLASESLRLNEAFNKWIVTRRPFVIAKCGL
                     SLDGRLNRPRHEQRWITDAVARRDVHRLRAQVDAILVGAETVRQDNPRLTVRGVKGAK
                     QPWRVVITRSGVLPKNARLFSDRWAKRTIVYRHKSLAAVLQDLGRQEIVSVLLEGGGR
                     LLGAALDGGLIDKVQIYLGPILTGGGVAAFAGRGAFSTQAAEHLEDVTYTEIGQSIRI
                     TGYLRNAAKELGE"
     gene            6051..6311
                     /locus_tag="VFO30_01440"
     CDS             6051..6311
                     /locus_tag="VFO30_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417978.1"
                     /translation="MKKLLLIAGIAGAFMFAATPSRAGVSVGIGIGFPVGYAYPYPPY
                     PYPYPYYAPGPVYFGPSFYWYHGYRVYYPRHRRYRYLPYYRY"
     gene            complement(6322..6903)
                     /locus_tag="VFO30_01445"
     CDS             complement(6322..6903)
                     /locus_tag="VFO30_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417979.1"
                     /translation="MGRQLNLIAAGIVLSIFATELSFAQGPPLAQGPPGQKPFAQGPP
                     GQKPFAQGPPGQNRQVRRPLDVRERWMQLPPQERQIFERNAERWLRMTPQQQNALRQR
                     EKMRRARLKSEADTLLHQSGLQLDQRRRDLFEARYMQERQRMERQLRQEFEAKRQREL
                     PALSERLKSEFQSPTSPGPRPTSAPAVSLSPRR"
     gene            complement(6879..7235)
                     /locus_tag="VFO30_01450"
     CDS             complement(6879..7235)
                     /locus_tag="VFO30_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417980.1"
                     /translation="MKRQDDVQLWDLLGRAVERKVSPFFARNVLRQIRQRPRFSERIA
                     RWLSWRQLVPASALAVAAITAIIFAHRVAGPQTRPDKTPDVMARINPQDYEVVADLDD
                     LLASDDSSLWDDNSTL"
     gene            complement(7308..9143)
                     /gene="rpoD"
                     /locus_tag="VFO30_01455"
     CDS             complement(7308..9143)
                     /gene="rpoD"
                     /locus_tag="VFO30_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET9417981.1"
                     /translation="MARPSKRKARRAPPKRRSLKRLGRANRVAAELRETNQSQQFSTL
                     KPPDIAALVRVTREVALAESSPVSGPADLPGGAEIQGRIRELIKLAKDQGYLTFEDLN
                     EALPEGVTDPDELDAILMRLRRMEIDIIEASEVDRYKDGKKDSEEEDEEEKADAKLDI
                     LDDPVRMYLKQMGQVPLLTREQEVEISKRIEEAEAMVQKHINRFGFIARAHIDLAQKL
                     SEGGERFDRVILDKKIESRERYMKMLPRLCKQVEKLSVSIARQYAELARNGATNESKH
                     QRAFERAVALLQKVYPRFYFKQKVTEEFVHLADEAYRSAVYLQEELAKRPRDAKKEKL
                     LTGKLRELENSLWLSLDAYAQQYQALKHWLRQALRAKTEMVEANLRLVISIAKKYTNR
                     GLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
                     MIETINKLMRVQKQLVQEYGREPTPEEVAEEILLPVDRVRAVLKMAQQPISLQSPVGE
                     SEETNFGDFIEDKGAENPSDMTAIVLLKEKIKDVLETLTERERQVLEQRFGLVDGYSR
                     TLEEVGRQFRVTRERIRQIEAKALRKMRHPTRIRQLAGFLEAPEA"
     gene            complement(9193..10995)
                     /gene="dnaG"
                     /locus_tag="VFO30_01460"
     CDS             complement(9193..10995)
                     /gene="dnaG"
                     /locus_tag="VFO30_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9417982.1"
                     /translation="MAIPSQTIEQVAAANDIVEVIGSYFPLKRAGANFKALCPFHQEK
                     TPSFHVSPQRQTFHCFGCGAGGSVFRFVMEYEHVDFPAAVRKLAARVGIPVAEERGGS
                     DEDRQHETRRALLKLQAEAAEWFHENLLKKEFAEPARQYLKQRGIDRPVAKNWQLGFA
                     PEAWDALLKWATARGYSREQLLQSGLVKARAEERPGNEVYDRFRGRIIFPICNDVGEV
                     IAFSGRLLQSDAQAAKYLNSPETLLFRKGAVLFGLHKSKRSLIEAKCAVVCEGQLDLI
                     ALFEAGITNVVAPQGTAFTPEQAHILKRYVDEVVLCFDADEAGQKAGERSLDPLLQHD
                     LIVRVAEIPPGEDPDSLIRREGKENFEQRIAAAREFFDYWLDRKMANIDPGSLGARMQ
                     LARELAQTVSHVHDSMMRGQVINQVSVRLGVPAPEFAALLPKQPRERASGNGQSEQTS
                     TPPRHDIAMLCLLALRDGAARNLLLEQNWRELLDQTPGAELLVRILESDLRLEDTASM
                     NAFMASLSPAEERLVSAWLLQKVPENPLAVAEGWWNGLRQALLRRHLQIAEGRLKLPQ
                     LSTGELLNLQKQILDLRQQLHEFSPFSSARSGDK"
     gene            11038..12435
                     /locus_tag="VFO30_01465"
     CDS             11038..12435
                     /locus_tag="VFO30_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417983.1"
                     /translation="MKMFLSIFGIVACVFAGHIAQGRDVIQKGDVVVVPLRGEISPSL
                     LMFLRRAEKLAETNGASAIIFEMNTYGGQLDAAEEISKVLNRATIPTYTFINTNAGSA
                     GALIALATPHIYMAPVSAIGAAAPVLPTGEDLPLTQREKTISYWSALIRSSAVKNGHN
                     PDVGKAFMNKDKEVKIGDRVVHPKGTVLTLNAQEATEKINGKPLLADGIADSIVDLIE
                     KAGLKGNSVALNPTGFEQLAFWITALAPLLLLGGIIGAYLEFKIPGASLPGIISAICF
                     ALFFLGHYLAGLAGWEVVGLFVLGMVLVIIEILFFAHSTIVFGVVGVFLMLASLLWAM
                     IDRYPGETFLPTGRMLAMPLLNLFLAMFAAAIVITLLARYLPRTSVYRRFALMTSHPG
                     GPSLAGAPRQFATALSLAPGMQGVSLSILRPSGKARFADHVIDVVTQGEFVAPNTPVT
                     IVESDGMRVVVKATS"
     gene            complement(12437..12637)
                     /gene="thiS"
                     /locus_tag="VFO30_01470"
     CDS             complement(12437..12637)
                     /gene="thiS"
                     /locus_tag="VFO30_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET9417984.1"
                     /translation="MTISLNGEKVDARGAQDITQLVERFQLAPQTILIEHNGVALHRH
                     EWPQRLLREGDQIELLRVVAGG"
     gene            complement(12634..13758)
                     /gene="thiH"
                     /locus_tag="VFO30_01475"
     CDS             complement(12634..13758)
                     /gene="thiH"
                     /locus_tag="VFO30_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HET9417985.1"
                     /translation="MSFAETLDNLPLAKSPLVRRFEELIAPKSDGAFEQMAETAHVLT
                     LQNFGRTMRLFAPLYLSNECINNCRYCGFSRDNPILRVTLSADELLAEARHLAGQGFR
                     QILLVAGEHPKFVSRDYLAECVRSLIPDFSSISIEVGPMEVEDYVPIVQAGAEGLVVY
                     QETYNRAVYAEMHTAGPKRDFNFRLDCVERGYAAGFRRLGIGALFGLSRWQGEAVALA
                     AHTEYLLKRCWQAQITVSLPRLRPAAGGFRPLFSMTDRELAQLVCAFRITFPQVGIVL
                     STRERASLRDTLVSLGVTMMSAGSHTEPGGYTRQGGEHLHRTVRGRIVAPEYQDGPDE
                     LATGQFEISDDRSPAEIVAVLRGCGLEPVWKDWDQALCGA"
     gene            complement(13936..14643)
                     /locus_tag="VFO30_01480"
     CDS             complement(13936..14643)
                     /locus_tag="VFO30_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417986.1"
                     /translation="MKKCLLSVSGLLALASFTFAGPETYSGKEMKTTMAPPPCPQWYA
                     DTEWNVDLWGTYVFTDTKWMNDKYLEADHAWGGGIDFKYFFMRYFGVGIEGWAVDANQ
                     ARVHSFFDFSDGIGERFVTRDRGAVGAVLGTLTLRYPIPCTRFAPYVFGGGGGIFGGG
                     QRPHNVLEAETGEGFDIIRTHGFSDSETEGIGQFGGGLEVRITPHIGWMADFSWNVVD
                     GPENNFGMVRSGLNFAF"
     gene            complement(14794..16365)
                     /locus_tag="VFO30_01485"
     CDS             complement(14794..16365)
                     /locus_tag="VFO30_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9417987.1"
                     /translation="MIDKVRKILGLSSRKTGNKLILSIEKLERRVALLENGQVEEYNV
                     ERTGSRNIVGSVYKGKVKNIEMGLKAMFVDIGLERNAFLHFWDAIPAALDAGIEEVDR
                     PSRKQKKRITAKDIPSIYPVGSDVIVQVTKGPIGTKGPRVTTNLSFAGRYLVLMPFSD
                     RSGISRKIEDPKERERLRKVLRELDIPDSIGVIIRTVGEGQRARYFVRDLRFLLDQWA
                     KVEQQIRQNPTPCRVFEEPDLVERTVRDFLTEEIDEVLCDDRAATDRMMEIVGQISRR
                     SRNRIRFYDGAAPIFETYGVQKQIDDAFHRQVWLKCGGYIVIDETEALVAIDVNTGRN
                     KGGRDVEKTILQTNLEAAAEIARQLRLRNIGGLIIGDFIDMKSRKDQQMVYNLMKERL
                     KRDKAKTYVLPISPLGLMEMTRQRAQESLSDTIYENCPYCGGRGAVKTSMTTSVELHR
                     TLNRVMRKYQDSIHDIRVILNPDVLKRLKEEDDELLVELERRYAGRLMFRGDPTYHHE
                     KYLITDANTGAELKP"
     gene            complement(16438..18702)
                     /gene="mrdA"
                     /locus_tag="VFO30_01490"
     CDS             complement(16438..18702)
                     /gene="mrdA"
                     /locus_tag="VFO30_01490"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9417988.1"
                     /translation="MNRRLSPRLRLQFLGLLMLIGMGALGLKLWWIQVAHGAEWTAQI
                     RGSSQATVRIPSVRGEIKDRNGLPLVQNRASYEVDFYLPEMVKGYRERFGQPPLTEYR
                     AIINGMPKDVKEPDIIRIVNDGIVPRLNDLDLARDYNSTQLQRHFRNDTEVPFSYIKD
                     IDFPTIAKFSEHDVGLPGVDIAIKPVRSYVYGALAAHLLGYVGAPDDVNREEARKYTF
                     YQSDVEGKSNIERVMDEYLRGKPGVRYLRRNAKGVIEGVLREDPPKQGANVFLTLDAR
                     IQAITEEALRAVSRAGAVVVDPNSGNILAMASVPSFDPNTFIPSIKAKDWKALQKDEG
                     DPLVNRAISALPPGSTFKLVTSLSGLRGSKNLANAKFSCGGGVSYGDHFFRCWVAEKH
                     YTHGTLGLADAIKVSCDSFFYQYGNAAGIHSIDIVGKMLGIGEESGLQLSGEQTGNMP
                     GPEWMQIHHPQERWSQAQTANVSIGQGYTLVSPLQLAMAYATAANGGTCYYPRLVDKV
                     LNQDGSPVLDEHGKPAVSQTPRVRSDLRREVSPQDIDLVRKGLWKVVNEDGGTGGRAR
                     LKDVVVAGKTGTAQASDRGHEENVAWFACFAPFDHPKYVVAVMVQGASGHGGEVAGPI
                     ATRILERTLAQDEGKFDMQVAWLAPGHKGNPLQLIKAVSYAGGNLGSEDEEGADTSQS
                     ADVQMATAEAAPDVEPEADAQGQVQRARVARTLPVATPAPRQRNFFERLFGIRRAPAP
                     VQPTPPPARRVRPR"
     gene            complement(18699..19241)
                     /locus_tag="VFO30_01495"
     CDS             complement(18699..19241)
                     /locus_tag="VFO30_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9417989.1"
                     /translation="MIFFLLLLVLVTIAQIGEFFIPPLAWLYNTHIYLVPLIVFYAAV
                     ALPVPLMLAVALYAGILLDALTVQVIGNKVEISLGWSILLYAVLAGLMHGLRPLFVRG
                     RWEIHAVLSGICTSVILLAQYLMITFRRGSIIFSPEVWWQIGGPGLIAMLMAPVVFWL
                     LHWIAYVTGFGYLSERSRFE"
     gene            complement(19238..20041)
                     /gene="mreC"
                     /locus_tag="VFO30_01500"
     CDS             complement(19238..20041)
                     /gene="mreC"
                     /locus_tag="VFO30_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9417990.1"
                     /translation="MSRTNFIALLIFGVILGYFLSFGAESTRKFQAGIYQLIAPFLSS
                     GSGLQKQITSVRTGLKTLEQLERESAQLRVENRELRATNQALRDVEREVNSLRHQLNY
                     RERSVFKLVPAVVVTRDSSTWWHTVTVNRGREDGIEGDMAVVTDEGLVGKTTTVASSI
                     SVVLLVSDENCKVAATVEGTREQGIVSGERVTSGLTPSLYLNFLSKQADLKPGQKVYT
                     SGVGGVFPSGLPVGVVKSFRVRELDGQAQLTPAVDLSHLEDVFIVTGRK"
     gene            complement(20144..21181)
                     /locus_tag="VFO30_01505"
     CDS             complement(20144..21181)
                     /locus_tag="VFO30_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9417991.1"
                     /translation="MFEGLFGWLSNDIGIDLGTANTLVYVKDQGIVLREPSVVAVQAG
                     TNKVLAVGDEAKRMLGRTPSNIVAVRPLKDGVIADFEVTEAMLRHFISKVHNRRVKAR
                     PRVVIAVPSGITEVEKRAVRESATHAGAREVYLIEEPMAAAIGVGLPVQDPAGNMIID
                     IGGGTTEVALISLSGIVFSRSVRVAGDELDEAIVQYMKRAYNLMVGERTAEEIKIKIG
                     SAYPTEKETSVEVKGRDLVAGLPKTLMITSQEVREALLEPISTIVDSVRITLERCPPE
                     LSADLVDRGLVLAGGGALLRGFDKLLSEETGLPVHVAEDPLSAVAEGTGKCLNELKFL
                     RQVASADRQWR"
     gene            21400..22044
                     /gene="rpe"
                     /locus_tag="VFO30_01510"
     CDS             21400..22044
                     /gene="rpe"
                     /locus_tag="VFO30_01510"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9417992.1"
                     /translation="MSVLVAPSILAADFSRLGDEVRNVERAGADWIHCDIMDGHFVDN
                     ISFGPGIVNLVRNQTKLPLDVHLMIEHADHYVPRFVAAGANSITVHVEPEAKHDVEKT
                     LAQIRAAGCRVGLTLNPETSFDLVEPYLERIDMLLIMTVHPGFGGQSFRADQMAKVKR
                     AKSLNDKINIEVDGGINAETARLSIQNGANVLVAGTSIFHTQDYAKAIRELRGA"
     gene            complement(22052..22456)
                     /locus_tag="VFO30_01515"
     CDS             complement(22052..22456)
                     /locus_tag="VFO30_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9417993.1"
                     /translation="MGLCEVLHFVQDHSSVAGVLNKYFVYMLTNRSRVVLYTGVTNSL
                     ERPLISRQHGQKHFVKCYKLDRLVYYEAFDRAHDAISREKEIRGWCREKKNELVRKLN
                     PKWRDLGKQLVGEPPYRSGTKSPQRGRLRGSG"
BASE COUNT         4588 a         6803 c         6341 g         4949 t
ORIGIN      
        1 actcgttaaa aatcgggcgg aaaatgtcgg tctcgtaatt agaaattgcc ccggagaaat
       61 ccgataaatt ctttgtcccc tgaatgatcg cggtcacgcg ctcaaaaccc attccggtat
      121 cgacgtgacg ctgtggcagc ggtgtgaatg tgccgtcggg attcgcgttg aactgaatga
      181 agaccagatt ccagatttcg aagcagcggg gatcgttctt gttgacgagc acaccgcgcg
      241 tgtcgccgtc caccgtaaga tcgacatgta gttccgaaca cggcccgcac ggaccggtat
      301 cgcccatcat ccaaaaattg tcggctttac cgccggtgag gacgtgaacc ttcgggtcaa
      361 gatcggcatc agcgaaaagt cgcgcccaat ggtcgtgcgc ctcctggtcg aattcggccg
      421 gttcgtcagg cccgggtttg taaaccgtgg cgtaaagtcg ttgcgccgga aatttccagc
      481 cctcgacaac gagttcccac gcccattcga ccgcttcttt cttgaaataa tcgccgaagc
      541 tccagttgcc gagcatctcg aaaaaggtgt ggtgataagt gtcgaggccg acatcctcga
      601 ggtcattgtg tttgccgccg gcgcgaatgc acttctgcgt gtcggcgacg cgcgctgggc
      661 tccatggcgg tttttgttgt cccaaaaaaa tcgggacgaa ctggttcatg ccagcgttgg
      721 tgaacagcag gttcggcgcg tcgggcaaaa gtgaggagga cggcacgatg gtgtgctgtt
      781 tctcgcgaaa gaaatcgaga aacgattgcc ggatttgcgc cgaggtcatg atgtcgatct
      841 tacgaagtta gttttaatca ggaatcaaca cgaatgaaca cgaattcaac tttggcgtat
      901 cgaacgtcca gtgcgacaca gtttcgcgcc gccggttaca agcgacctcg gagcttaccc
      961 gcgacccgaa tcagctctgc ggcagtttcc aagccgcgcg gaattcgcgg tcgatttgcg
     1021 cggcttcgta actacgacct tgcgcgttca agcaatcgcg caagccggcg agggcgcgcg
     1081 cgtcgcgcgg gttgcgatca aggtcggcgc ggaaaaccgt ttccgcatca gcgacgcgct
     1141 tgttggcgag cagcacgcgc ccgagcaacg ggcgcacggg cggataccac gtgggcggct
     1201 cgctgtaatt aagcgcgtcc tcggcggcaa ctgcgccttt caggaactcg accgttgcgt
     1261 tcgcatcgcg gtgactgccg gcgattgcag cggctaaaag actctcgtgc accttcaaca
     1321 ccgcggcggc ctggttcagt gggtcgtaca ttttgtcggc cggaattttt gcgctgagat
     1381 ctcgaaaact cttctgctcg ttttctgctt cagcgcttcg gccgacattg gcgagagcga
     1441 tgccgcggac ggaatgccag acggcatttg aaacggcaac tgatgggtca ggcttgggta
     1501 attccaggat gtcgctccag cgctcgaagg cgagcaaaat gaggatcggc gtcggcaaaa
     1561 atccctcgag ctccggcatc gacttcactc cgggcgcaac atttgccact aactgcgcag
     1621 cggcagtttt cgcttcggca aaatcgcccc gcatacagtc ggcatacgcg aggaagtgga
     1681 ggttatggct gtagtacatg agcgggtaaa ccccttgctc gtgcgtttcg cgaataaact
     1741 tttggtcggc ggccaccgcc agcttattgg aatgcgctgc cgcggcgtgg tcgccaacgc
     1801 gcgcgtagat gtgggccggc atgtgcacga gatgaccggc cgcgttcgcg agtttttcca
     1861 aacgcatcgc actcgccagc gcgcgctccg gattcggcga agcttcaatg gcgtgaatgt
     1921 aataatgatt cgcgccgaca tggttcgggt cccgtttaag gaccgactca agcacccaga
     1981 cgattcgatc ggtaccttcc gcaggtctgc cgtcagcagt ccagagatgc caggggttca
     2041 aattcatcat gctctcggca aacagcgttg cggcatcgag atcgtcggga tagcgcgccg
     2101 cgagtttggc catggcatcc ttgtaagcga tgtcgagtgc gtgaagatcg acatgtggat
     2161 cattcgaata gcgcgccgcg agcgcgttga tgtacgcgcg ctccggatcc gacgcgttgt
     2221 cctgcaacgc cagcgcatgc tgcactgcct cgtaggctgc tttctcgcgc gcgggatcca
     2281 ccggtaggtt gtaattgggt ccaagcgtca gcgcgacgcc ccaccacgcc attcccagct
     2341 gcgggtcgag ttgcaaggcg cgttcgaacg agcgccgcgc ctcgtcgtga ttaaacgcgt
     2401 aaactaactt caggccctga tcgaagaact gctgcgcctc gcgatttttt gtcgagaccg
     2461 gatggtgcaa atcgcccaaa cctctcatca accgcagcgg ctctggtttc gtgcgcccag
     2521 tcacagccac gacggcgcaa agcacgagac taaccgacag caggggccga aatgatccgg
     2581 cgaacatgtc gatcttgcga gcgcgcgagt taagttttct cttcgtccag cttggccttt
     2641 acgcccgact caatctcctc gtagagcgca cgatcattct tcaacacatc ttttgctgcg
     2701 tcgcgtccct gggcaagctg tgtgcctttg tagatcaacc aagaaccgcg tttctcgata
     2761 atctgtttct ccagggccag atcgagaagt gcgccggtga gggaaatccc ctcattgtac
     2821 atgatgtcga actcggcctc ggtaaagggc ggcgcgactt tatttttgac gactttgatc
     2881 cgggtgcgat tgccggtgac ggtgccatcg gtctgcttga tcgcaccgat gcggcgaata
     2941 tcgacgcgca tgctcgcgta aaacttcagc gccttgcccc caggcgtggt ctccggattg
     3001 ccaaacatga cgccgatttt ctcgcggatc tggtttgtga aaatgcaaca ggtgcgcgcc
     3061 ttggaaatga gagacgtcaa tttgcgcagt gccgcgctca tcagtcgcgc ctgcgcgccg
     3121 acggtcgcgt caccgatttc gccctccaat tccgctcgcg tgacgagcgc cgcgaccgaa
     3181 tcgacgacaa ttacgtccag cgaatttgag cggatgagcg tttcacaaat tcgtagcgct
     3241 tcctcgcccg agctcggctg cgacactagc aagtcgtcaa ggctaacgcc aagtttacgc
     3301 gcgtactgcg gatcgagcgc atgctcgaca tcgatgaagg ccgcgaggcc gccgcgtttc
     3361 tgcgcctggg caatgaccgt cagcgtgaga gtggtcttgc cggaagattc aggtccaaat
     3421 acctccacga cgcggccacg gggaaaaccg ccgaccccca atgcgcgatc gatcaagatg
     3481 ttgcccgtgg gaatgacgtc gatgtcgacg attttctcgt cgcccaggcg catgatcgcg
     3541 ccttcgccgt agtctttggc gatctgctga atggcgagat cgaggttttt gttgcgtgcg
     3601 accgtgagct tgtcctcgcc ggttttctcc gctgtcgcag ttttggtcgc cataactccg
     3661 cttcaatcta actaagtcgc gccggtcgca ttcgccaagc acaaaagcgt cacagaaccg
     3721 gcagaagcac gtaaaaaaag ccgcgggcct cacgacccgc ggcttgcact gaaacggtgt
     3781 ctgtctttac gagacggtga ttttcctcgg cttgaggtgc tcggcctttg gcagcgtcaa
     3841 cgtcagaatc ccttggtcca tatttgcgct gattctattc gcgtcgattg agggatcgag
     3901 ctcaaaaacg cgacggaagt tttcgcgttg caactcataa tggatcaacg tgccgtcgat
     3961 cgtcggcagt gaacgccggc cgatgatgct cagctcgttg ttttccatcg agatttcgag
     4021 cccttccttg gtcacaccgg gcatttcaat acgcagcgtg tagccgtcgt tctcttcgac
     4081 tacactggct gttggcgcaa cgaattgctc ggcttgcgca ccgcgcgctt cgatctcgcg
     4141 attgtctcga acggttgtgt tcataaggtt ttcctttcgt tcaaaatggc aggcgctgag
     4201 ttaatcggcg cggaccttga tctgtttcgg tttggcctcg tcggcctttg gcagcgttac
     4261 gataagaatg ccatcccgat aggtcgcgct cactttgttc gcatcgaccc ggacggggag
     4321 gctgatactg cgacggaatt tgccgacata gcgttcggtc cgttcggcct tgtcgccgtt
     4381 gcctgcctcg gctttgcgtt caccagcaat cgtgagggtg ccattatgca aggagatttc
     4441 gatttcttct ttgcgcatgc ccggcagctc gaccaccgca acgacagtgt cgttattctg
     4501 gtacaaatcg agcgcaggag cccagccgct caaaagctgc ccctgccgtc caaaggtgga
     4561 ccagaacggc aactctaaga acgcattcaa gtcgtcccgt aggctgctcc agcgatctgc
     4621 ccacggccaa gctgctgatt caggcgtctg ataacgaatt agactcatag gtaaggttcc
     4681 tttctagttt gaaattgtgc actcaactca aagctgtcga catgccacgc aagggggcaa
     4741 ccgaggctgt gtctaataac ctgcttgctc ggatatggtt acagagtttg tcgcgccttg
     4801 aagacggcga cctagtcgat ccaacatgtc acagtttacc ctttcccggc tacgttcgcg
     4861 gagagacaga tttgcacaag atttgggtca taactggtgc cgcgaaaaca aacgttggct
     4921 atcatcgccc gccgccgata ttcgtctgcc taatgaactt cgcagacgcc gatgagcggt
     4981 tcatgcgtgt ggcgttgaat gaagccagga agagtttggg ccgaaccagt ccaaatcccg
     5041 ctgtcggagc tattttggtc tgtggcggcc gtattctggc gcgtgctcac cacactcgcg
     5101 ccggcgagcc gcacgccgaa atcgcatgtc ttcacaaact cggcggcccg gttccagcgg
     5161 atgcgacgtt gtacgtgacg ctcgagccgt gttcgacaac cggcaaaacg ccaccatgca
     5221 ccagagcgat tattgatgcc ggcgttcgga cggtcgtcat cggcgcagtg gatgtaaacc
     5281 cgcgtcacag cggccggggt atttccgagc tgcgaaacgc cggagtgatg cttcgcaccg
     5341 gtgtcctggc gagtgagagc ctgcgtctaa acgaagcgtt taacaaatgg attgtgacgc
     5401 gtcggccgtt cgtaattgca aagtgcggtc tgagtttgga tggaaggctg aaccggcctc
     5461 gccacgaaca acgctggatc acggatgcgg tcgcgaggcg tgatgtgcac cgattgcgcg
     5521 cacaagtgga tgcgatcctc gttggtgctg aaacggttcg gcaggacaac ccgcggctga
     5581 ccgtgcgcgg tgttaagggt gcaaagcagc cgtggcgggt ggtgatcacc cgctcaggcg
     5641 tgctgcccaa aaacgcgcgc ttattttcgg accgctgggc gaagcggacg attgtttatc
     5701 ggcacaaatc tctcgcagcg gtgctgcaag atcttggccg gcaggaaatc gtcagtgtct
     5761 tactggaagg cggcgggcga ctgctcggtg cggcactgga tggcggctta atcgacaagg
     5821 tacaaattta tcttgggccg atcttaaccg ggggcggagt cgcggccttt gcggggcgcg
     5881 gagcattttc aacccaagct gccgagcatc tcgaggacgt tacctacacg gaaattgggc
     5941 agagcattcg catcacaggt tatctgcgca acgcagcgaa agaactcggc gaataaattg
     6001 cgaaacttcg gagcgcagtg aaagttgaat ggagcggagg gtaaaaagaa atgaagaaac
     6061 tgttgttgat tgcagggata gctggcgcgt tcatgtttgc cgcgacgccg tctcgtgctg
     6121 gagtctcggt tggaatcggt atcggattcc ccgtaggata cgcttatcca tacccgccgt
     6181 atccgtatcc ttatccgtac tacgcgcctg ggccggttta tttcggaccg agcttctact
     6241 ggtaccacgg gtaccgcgtc tactatcccc ggcaccgacg ttaccgctat ttgccctatt
     6301 accgttacta gctgagtgcg ctcagcgtcg cggcgataac gacactgcag gtgcagaggt
     6361 tggtcgcgga cctggcgatg tgggggattg gaactcgctc ttgagtcgtt cgcttagagc
     6421 cggcagttcc cgctgccgtt tggcctcgaa ctcctgacgg agctgccgct ccattctttg
     6481 ccgctcctgc atgtagcgcg cttcgaataa atcccgtcgc cgctggtcaa gttgcagacc
     6541 ggactggtgc aagagcgtat cggcctcgct tttcagccgg gcgcgccgca tcttttcgcg
     6601 ctggcgcagt gcattctgct gctggggcgt catgcgcagc cagcgttctg cattgcgctc
     6661 gaagatctgt cgttcttgcg gaggaagctg catccagcgc tctcgcacgt cgagcgggcg
     6721 ccgtacttgg cgattttgac ccggtggacc ctgcgcgaaa ggtttttgac ccggcggacc
     6781 ctgcgcgaaa ggtttttgac ccggcggacc ctgcgccaaa ggtggaccct gcgcgaaact
     6841 gagttcggtg gcgaagatcg acaatacgat ccccgccgct ataaggttga gttgtcgtcc
     6901 cacaagctgc tatcatccga agcgagcaga tcgtccagat cagccaccac ttcataatcc
     6961 tgtggattga ttcttgccat cacgtccggc gttttgtccg gccgggtctg cggcccggcc
     7021 acacggtgtg cgaaaatgat cgcggtgatc gccgcaaccg ccaaggccga cgcagggacg
     7081 agttgtcgcc aactcagcca ccgtgcgatg cgttctgaaa atcgcggccg ctgccggatc
     7141 tgtctcagaa cattacgggc aaagaacggc gagaccttac gctcgaccgc gcggccaagg
     7201 agatcccaca attgcacgtc gtcttggcgt ttcataaatt tttccgccga ttaaactact
     7261 tacgccggcc gaagttgcgc gcaaaaacct gttgctaaga gcgacattta cgcttcggga
     7321 gcttcgagaa aaccagccag ctgccgaatt cgcgttgggt ggcgcatctt gcgcaacgcc
     7381 ttggcttcga tctgccggat ccgctcgcgc gtaacacgga attgccggcc cacttcctcc
     7441 aaggtgcggc tgtaaccatc caccagcccg aagcgctgct cgagcacctg tcgctcgcgt
     7501 tcggtcaacg tctcaagcac atccttaatt ttctccttta gtaagacgat agccgtcatg
     7561 tcgctcgggt tctcggcacc cttgtcctca atgaaatcgc cgaagtttgt ctcttcgctc
     7621 tcgcccaccg gtgactgcaa cgagatcggc tgctgggcca tcttcaaaac cgcgcgcacc
     7681 cgatcgactg gtagcaaaat ctcctccgcg acctcctcgg gcgtcggttc ccgcccgtac
     7741 tcctgcacga gttgtttttg cacccgcatc aacttgttga tcgtctcgat catgtgcacg
     7801 ggaatgcgaa tggtgcgggc ctggtcggca atggagcggg tgatcgcctg ccgaatccac
     7861 caggtcgcgt aggtcgagaa cttatatccg cgacgatact cgaacttctc gactgccttc
     7921 atcagtccca tgtttccctc ctggatcagg tcgagaaatg acaaaccgcg attggtatat
     7981 ttttttgcga ttgaaatcac caggcgcaaa tttgcttcca ccatttcggt ttttgcgcgc
     8041 aacgcctggc gtagccagtg tttgagcgct tgatattgct gcgcgtaggc atcgagcgac
     8101 agccacaggc tattctccag ttcgcgcagc ttgcccgtga gcagcttctc ttttttcgcg
     8161 tcgcgcggcc gcttcgccag ctcttcctgg aggtaaacgg cgctgcgata agcttcgtcc
     8221 gccaaatgca cgaactcttc ggtcaccttt tgtttgaaat aaaagcgcgg atacaccttt
     8281 tgcagcaaag ccactgcgcg ttcgaaagcc ctctggtgtt ttgattcgtt cgtcgcgccg
     8341 ttgcgggcaa gctcggcata ctggcgcgcg atcgagacgc tcagcttttc cacttgcttg
     8401 cataaacgcg gcagcatttt catgtagcgc tcacggctct cgatcttctt gtccagaatc
     8461 actcgatcga accgctcgcc cccctcgctc agcttctggg caagatcaat gtgcgcccgc
     8521 gcaataaagc cgaagcggtt gatgtgcttc tgcaccatcg cctcggcctc ttcgattcgt
     8581 ttcgaaatct caacttcctg ctcgcgcgtg agcaacggca cctggcccat ctgtttcaaa
     8641 tacatccgga ccgggtcgtc gagaatgtcg agcttggcgt ccgctttttc ctcctcgtcc
     8701 tcttcttcgc tgtccttctt gccgtccttg tagcggtcga cttcggatgc ctcgatgatg
     8761 tcaatttcca ttcggcgcag ccgcatgaga atcgcatcca attcgtccgg gtcggtgacc
     8821 ccttcgggca gcgcctcatt gaggtcctca aacgtgagat agccttggtc cttcgcgagt
     8881 ttaatcagct cgcgaatgcg gccctgaatc tcggcgccac cgggtagatc agccggtccg
     8941 cttactggcg agctttccgc cagtgccact tccctggtca cgcggaccag cgccgcgata
     9001 tcgggcggtt ttaacgtact gaactgctgc gattgatttg tttcccgcaa ctcggcagcg
     9061 acgcgattgg cgcggccaag gcgtttcaga ctccgccgct tgggcggcgc gcgccgcgct
     9121 tttcgtttcg aaggtctggc caagttcgag aggtgatgga ttgaccgagt tacctgcccg
     9181 ttagatcgac aatcacttgt cccccgagcg ggcagacgaa aacggcgaaa actcgtggag
     9241 ttgctggcgc aggtcaagaa tttgtttctg caggttcaac agctcgccgg tgctcagttg
     9301 cggcagcttc aaccggccct cggcgatctg aaggtgccgc cgcaagagtg cctggcgcag
     9361 cccattccac cagccctcgg cgacggcgag cggattctcc ggcacttttt gtaacagcca
     9421 ggccgaaacg agccgctcct cggctggcga cagactggcc atgaaagcat tcatcgaggc
     9481 cgtatcctca agccgaaggt cgctttccaa aattcgcacg agcaattctg cgccgggtgt
     9541 ttggtccagt aattcacgcc aattttgctc cagaagaaga tttcgtgccg cgccgtcgcg
     9601 caaagccagc aggcagagca tcgcgatgtc gtgccgcggt ggcgtgcttg tctgctccga
     9661 ctgtccgttg ccggacgcgc gctcccgcgg ctgtttcggg agaagcgcgg cgaactccgg
     9721 tgccggcacg ccgaggcgga cactcacctg attgatcacc tgcccgcgca tcatcgagtc
     9781 atgcacatgc gagacagtct gggctaactc gcgggcgagc tgcatcctcg cgcctaacga
     9841 acctgggtcg atattcgcca ttttgcggtc gagccagtaa tcgaagaact cgcgtgccgc
     9901 ggcgatgcgc tgctcaaagt tctcctttcc ttctcgccga atcagcgagt cgggatcttc
     9961 tccgggcggg atttcggcga cgcggacgat gagatcatgc tgtaaaagcg ggtcgaggga
    10021 gcgttcaccg gccttctggc cagcctcgtc cgcatcgaaa cagagcacca cttcatcgac
    10081 atagcgcttc aaaatgtgcg cctgctcagg cgtaaaggcc gtcccctgcg gcgcgaccac
    10141 gttcgtgatc cccgcttcga acagcgcgat caaatcgagc tgcccttcgc aaaccactgc
    10201 gcatttcgcc tcgatgaggc tgcgcttcga tttgtgcaga ccgaacagca cggcgccttt
    10261 ccgaaagagc agtgtttcgg gggaattcaa atatttcgcc gcttgcgcgt cgctttgcag
    10321 caagcgcccg ctgaacgcaa tgacctcgcc gacatcgtta cagatgggaa agataattcg
    10381 tcctcgaaaa cgatcgtaaa cttcgtttcc aggacgctcc tcagcacgcg ctttcaccag
    10441 tccgctctgc agcagttgct cgcgcgaata accgcgcgct gtcgcccatt tcagcaatgc
    10501 gtcccatgct tccggcgcaa aaccgagctg ccagtttttg gcaactggtc gatctattcc
    10561 gcgctgcttg agatattggc gtgccggctc ggcgaattcc ttcttgagca ggttttcatg
    10621 aaaccattcc gccgcctcgg cctgcaactt caacaaagcg cgacgcgttt cgtgttgtcg
    10681 atcttcgtcc gacccgccgc gctcttccgc caccggaatg ccgacacgcg ccgccagttt
    10741 gcgcaccgct gctggaaaat cgacgtgctc gtattccatc acgaagcgaa acacgctgcc
    10801 ccccgcaccg cagccgaagc agtgaaacgt ctgccgctgc gggctgacat ggaacgaggg
    10861 cgtcttttcc tgatgaaacg ggcagagcgc cttgaagttc gcaccggcgc gtttgagcgg
    10921 aaaatacgag ccgatcactt cgacgatgtc gttagccgcc gcgacctgct cgatggtttg
    10981 tgatggaatt gccacaggcg aatggcaaac ttagccagcg cgccacgagt tgaaaacatg
    11041 aagatgttct tgtcgatctt tggaattgta gcctgcgtct ttgcaggtca catcgcgcaa
    11101 gggcgtgacg tcattcagaa aggggacgtt gtcgtcgtgc cgctgcgcgg cgagatttcg
    11161 ccttcactgc tcatgttttt gcgccgcgcg gagaagctcg cagagaccaa tggcgcgtcg
    11221 gcgatcattt tcgagatgaa cacctacggc ggacagctcg atgccgccga ggagattagc
    11281 aaggtgctga atcgcgccac gattccgacc tacacgttca tcaatacaaa cgccggctca
    11341 gccggcgcgc tcatcgcgct cgccacgccg cacatttaca tggccccggt gagcgcgatc
    11401 ggcgcagcgg cgccggtttt gcccacgggc gaagaccttc cgctgaccca gcgcgagaaa
    11461 accatttcct actggagtgc gctcatccgc agttccgcgg tcaagaacgg acacaatccc
    11521 gacgttggca aagcgttcat gaacaaggac aaagaagtaa agatcggcga ccgcgtcgtc
    11581 catcccaagg gaacggtact gacgttgaac gcgcaggaag cgactgagaa gatcaatggc
    11641 aaaccgctgc tcgccgacgg catcgcggac tctattgtcg atcttattga gaaggccggg
    11701 ctgaaaggca actcagtcgc gctcaatccg accggattcg aacagctcgc cttctggatc
    11761 accgcgctgg cgccgctgct gttgctcggc ggcatcattg gcgcctatct cgagttcaaa
    11821 attcccggcg caagtttgcc cgggattatc tccgcgatct gcttcgcgct gttctttctc
    11881 ggccattatc ttgccggact tgctggctgg gaagtagtcg gtctgttcgt tctcggaatg
    11941 gtgctcgtca tcatcgagat cctcttcttt gcccattcca cgattgtctt cggcgtggtt
    12001 ggcgtttttc tcatgctcgc ttccttgctc tgggccatga tcgatcgtta cccgggcgaa
    12061 acatttttgc cgaccggccg gatgctcgcg atgccgctgc tcaatctctt tctcgcgatg
    12121 ttcgccgcgg cgatcgtgat cacattgctg gcgcgctacc tgccacggac aagcgtctat
    12181 cgccgcttcg ctttaatgac ttcgcacccg ggaggtccat cgctggccgg tgcgccgcgc
    12241 caattcgcaa cggcgctgtc gctcgcaccg ggcatgcagg gcgtctcatt gtcgatccta
    12301 cgcccgagtg gcaaagcgcg ttttgccgat cacgtcatcg atgttgtcac gcaaggcgaa
    12361 ttcgtcgcgc cgaacactcc cgtcacgatc gtcgaaagcg acggcatgcg cgtggtggtg
    12421 aaggcaacgt cgtagcctac ccgccggcga cgacgcggag gagttcaatc tgatcgcctt
    12481 cgcggagcag tcgctgcggc cattcgtgcc gatgcaacgc aacgccattg tgttcaatca
    12541 ggattgtttg cggcgcgagt tggaaacgct cgacgagttg cgtgatgtct tgggcgccgc
    12601 gcgcgtctac cttttcgcca ttgagtgaga tcgtcatgct ccgcaaagtg cctggtccca
    12661 gtctttccac accggctcca aaccgcaccc gcgtaacacg gcgacgattt ccgcaggcga
    12721 acggtcgtcg ctaatttcaa actgtccggt ggcgagctcg tctgggccgt cctgatattc
    12781 gggcgcgacg atccgtccgc gcacagtgcg atgcagatgt tcgccgccct ggcgagtgta
    12841 gccgccgggc tcggtatgac tgccggcact catcatcgtc acgcctaacg aaacaagcgt
    12901 atcgcgcaag gacgcgcgct cacgggtgct gaggacgatg ccaacctgcg gaaacgtgat
    12961 gcgaaacgcg caaaccagtt gcgccaactc gcgatcggtc atcgaaaaga gtggccggaa
    13021 accgcccgct gcgggacgta atcttggtag actaacggtg atctgcgcct gccagcaacg
    13081 tttcaacagg tattcggtgt gcgcggcgag cgcgacggct tccccctgcc agcgcgacaa
    13141 accgaaaagc gcgccgattc ctagccgacg gaaaccggcc gcgtaaccgc gctcgacgca
    13201 gtcgagacgg aaattgaaat cgcgtttcgg gccggccgtg tgcatctccg cgtagaccgc
    13261 gcgattgtaa gtctcctggt aaacgaccag accctcggcc ccggcttgaa cgatcggaac
    13321 atagtcctct acctccatcg ggccgacttc gattgaaata gatgaaaaat caggtatgag
    13381 cgagcgcacg cattcagcca ggtaatcgcg gctgacgaac ttcgggtgtt caccggcgac
    13441 gagcagaatt tggcggaaac cctgcccggc gagatgccgc gcttccgcga gtaattcatc
    13501 cgcgctgagc gtgacccgaa ggatcgggtt atcgcgcgag aagccgcagt agcgacaatt
    13561 gttgatgcat tcgttcgaga gatacagcgg tgcgaagagg cgcatggtgc gcccaaaatt
    13621 ctgcagcgtc agcacgtggg cggtctccgc catctgctcg aaggcgccat cggatttcgg
    13681 cgcgatcagt tcttcgaatc gtcgcacgag cggcgatttt gcgagcggca gattgtccag
    13741 cgtttcggca aatgacatca aagtaatttc gcgggtggcg cacgatccgc aaatcccgta
    13801 gcgatgcgcc tgtgatccct cgtcgggcag ccggaagcga ggctacagct gattgtctat
    13861 tctcccgcaa aggcgaaaaa caaagcggcg gaaccgaaaa gtcggttccg ccgcgtaaag
    13921 gcgcgaatgt cgatattaga acgcgaagtt caacccgctg cgaaccatgc cgaagttgtt
    13981 ctccggcccg tcaacaacgt tccaactgaa atcggccatc catccgatgt gcggtgtgat
    14041 gcgtacttcg aggccgccac cgaattgccc gattccctcg gtttcgctat cggaaaaccc
    14101 gtgtgtccgg atgatgtcga acccttcacc cgtttcagct tcaagcacgt tgtgcggtcg
    14161 ttggccaccg ccaaagatcc cgccgccccc gccaaaaacg tagggcgcga aacgagtgca
    14221 tgggatcgga tagcgcaagg tcaaagttcc gagcacagca cccactgcgc ctcggtcgcg
    14281 cgtgacgaag cgctcaccga ttccgtcact gaaatcgaag aaggaatgga cccgcgcctg
    14341 gttcgcgtcg accgcccagc cttcgatccc gacaccgaag tagcgcatga agaaatattt
    14401 gaaatcgatg cccccgcccc aggcatggtc ggcctcgagg tatttgtcat tcatccactt
    14461 cgtgtcggta aacacgtagg tgccccaaag atcgacattc cattcggtat cggcatacca
    14521 ctgcgggcat ggcggggggg ccatggtggt tttcatttct ttgccggagt aggtctcagg
    14581 cccggcaaaa gtaaatgacg caagcgccaa cagaccgctg acgctcaata agcacttttt
    14641 catcttttct ccttttgttg tggttaagtt ctcccgccct agccgaagcc gaaagcggaa
    14701 tcatattcta aaacgaaacc cagtccgttc ccggatgctt cgcccattta accaaagaat
    14761 caccgagcgc aagtgggagt tggggttttt tgcttagggc ttgagctcgg ccccggtgtt
    14821 cgcgtccgtg atcaagtatt tttcgtggtg gtaagtggga tcgccgcgga acatgagccg
    14881 gccggcatag cgccgctcca actccacgag cagttcgtcg tcctcctctt tcagtcgctt
    14941 caaaacgtca ggattcagga ttacccggat atcgtgaata ctgtcctgat atttacgcat
    15001 cacccggttg agcgtgcgat gcaattccac gcttgttgtc attgacgttt tgactgcacc
    15061 gcggccgccg caatacggac aattttcgta gatcgtgtcg ctcaaacttt cctgcgcgcg
    15121 ctgccgcgtc atctccatca gcccgagcgg ggagatcggc aggacgtagg ttttggcctt
    15181 atcccgtttc agtcgctcct tcatcagatt gtaaaccatc tgttggtcct tgcggctttt
    15241 catgtcgatg aaatcgccaa tgatgaggcc gccaatattg cgcagccgga gttgccgcgc
    15301 gatttccgcg gcggcttcga ggttcgtttg caggattgtc ttttcaacat cgcggccgcc
    15361 tttgttgcgg ccggtgttta cgtcaatcgc aacaagcgct tcggtttcat cgatcacgat
    15421 gtagccaccg catttgagcc atacttgtcg atgaaacgcg tcatcgattt gtttctgcac
    15481 gccataggtc tcaaagatcg gcgcggcgcc atcgtaaaac cggatgcgat tacgcgagcg
    15541 acgggagatc tggccgacga tttccatcat gcgatcggtc gccgcgcgat catcgcaaag
    15601 cacttcatcg atctcttcgg tcaaaaaatc gcgcaccgtt cgctcaacca ggtcgggctc
    15661 ttcgaacacg cgacagggcg tcggattttg ccggatctgc tgttcaactt tcgcccattg
    15721 gtcgagcagg aaccgaagat cgcgcacaaa gtagcgggcg cgctggcctt cgccaaccgt
    15781 gcggataatc acgccgatgc tgtctggaat atccagttcg cgcagaactt tgcgcaggcg
    15841 ctcccgttct tttgggtcct cgattttgcg cgaaatgccg ctgcggtcgc tgaacggcat
    15901 gagcacgaga taacgaccag cgaaactgag attcgttgtc acgcgcgggc ctttcgtccc
    15961 aatcggacct ttggtcacct ggacgatgac atccgagccc accgggtaga tgctcgggat
    16021 gtccttggcc gtaattcgct ttttctgctt gcgcgacggg cggtcgactt cctcgatgcc
    16081 ggcgtcgagc gcggcgggta ttgcgtccca gaaatgcaga aacgcgttcc gctccaagcc
    16141 gatgtcgacg aacatcgcct tgagacccat ctcgatgttc ttgactttgc ccttgtagac
    16201 gctgccgaca atgttgcgcg agccggtccg ctcgacgttg tattcctcaa cctgcccgtt
    16261 ctcgagcaac gccacgcgac gctcgagttt ttcgatgctc aggatgagtt tgttgccagt
    16321 cttgcgcgaa gagagaccta aaatttttcg taccttgtcg atcataaaat ggagagagtt
    16381 gcgcgatcag aaccgcagcc gctgacgcag tgctgagccg agtatctgat cgaaagttca
    16441 tcggggtctt acccgccgcg ccggcggcgg ggttggctga accggtgctg gagcacggcg
    16501 aattccaaag agacgttcaa aaaagttacg ctgccgcgga gctggtgtgg cgactgggag
    16561 cgtacgcgct acgcgtgccc gctgcacttg gccctgagca tccgcttccg gctcgacatc
    16621 gggcgccgcc tcagccgttg ccatttggac atcggcgctt tggctggtat cggcgccttc
    16681 ttcgtcctcg ctgccgagat taccgccagc gtagctcact gctttgatca attggagcgg
    16741 attgccctta tgtccgggtg cgagccaggc tacctgcata tcgaactttc cttcatcctg
    16801 cgcgagagtc cgttcgagaa tgcgtgtagc gatcgggccc gcgacctcgc cgccgtgccc
    16861 agaggcgccc tgcaccatca ctgcgacgac atacttcgga tggtcaaatg gcgcgaaaca
    16921 cgcaaaccat gccacgtttt cctcgtggcc acggtcgctg gcctgcgcag tgccagtttt
    16981 cccagccacc acaacgtctt tcaaacgcgc tctgccaccc gttcctccgt cttcgttgac
    17041 aactttccag agccctttgc ggacaagatc gatatcctga ggtgaaactt cccgccgcag
    17101 atcggagcgg acgcgcggtg tttgcgaaac ggcaggtttg ccgtgctcgt cgagcaccgg
    17161 cgagccgtcc tggttcagaa ctttatcaac caggcggggg taataacacg ttccaccgtt
    17221 ggcggccgtg gcataagcca tcgccaattg cagaggcgaa acaagcgtgt agccctggcc
    17281 gatcgaaaca ttagccgttt gcgcctgcga ccatctctcc tgcggatggt gaatttgcat
    17341 ccactcaggc cccggcatat tgccggtttg ttcacctgac agttgcaggc ccgattcttc
    17401 gccgattccc aacatcttgc caacgatgtc aatcgagtga atgccagccg cgtttccgta
    17461 ctggtagaaa aacgagtcgc atgaaacctt gattgcatcg gcgagtccga gggtcccgtg
    17521 ggtgtaatgt ttttcggcta cccagcatcg gaaaaagtga tcgccgtaac tcacccctcc
    17581 gccgcagctg aatttcgcgt tggcgaggtt cttggaaccg cgcagcccgg agagcgatgt
    17641 caccagcttg aaggtcgatc ccggtggcag cgcgctgatg gcacggttaa ccagcggatc
    17701 gccttcgtcc ttttgcagcg ctttccaatc cttggctttg atactgggaa tgaatgtgtt
    17761 tggatcgaac gacggcaccg aggccatggc cagaatgttg ccgctgtttg gatcgacaac
    17821 cactgcgcca gcgcgactaa ccgcacgaag cgcttcctcc gtgatcgcct gaatacgcgc
    17881 atctaatgtg agaaagacgt tcgcgccttg cttcggcgga tcttcgcgga gcacgccctc
    17941 aatcacaccc ttggcgttgc gacgcaaata acgcacgccc ggtttgccgc gcagatattc
    18001 gtccatcact ctctcgatgt tcgatttccc ctccacgtcg ctctgataaa acgtgtactt
    18061 gcgtgcttcc tctcgattca cgtcgtccgg cgcaccgaca tagccgagca gatgcgcggc
    18121 gagcgcacca tagacgtagg agcgtaccgg cttgatggcg atgtctactc caggcaaacc
    18181 gacgtcgtgc tcggaaaact tcgcgattgt cggaaaatcg atgtccttga tgtaggaaaa
    18241 cggcacctcg gtgtcgttgc gaaagtggcg ctgcagctgg gttgaattgt aatcacgcgc
    18301 caaatcgaga tcattcagcc gcggaacaat accgtcattg acgatgcgaa taatgtcggg
    18361 ctccttcacg tctttcggca tgccgttgat gatggcgcgg tactcggtga gcggcggctg
    18421 gccgaagcgc tctcgatacc ccttcaccat ctcgggcaga taaaaatcga cttcgtagct
    18481 cgcgcggttc tgcaccagcg gcaggccgtt ccggtctttg atctcgccgc ggacggacgg
    18541 gatccgcact gtcgcttgcg aactgccgcg aatttgcgct gtccattccg cgccgtgcgc
    18601 cacctggatc caccagagtt tcagcccgag cgcgcccatg ccgatgagca tgagcaagcc
    18661 gagaaattgc agccgcaaac gcgggctcaa acgccgattc attcgaaccg actcctttcc
    18721 gataaatagc cgaagccggt gacatacgcg atccagtgca acaaccagaa aaccaccggc
    18781 gccatcagca tcgcaatcag acccgggccg ccgatttgcc accacacctc aggtgaaaaa
    18841 ataatggagc cgcggcgaaa agtgatcatg agatattgcg ccaaaagaat gaccgaagtg
    18901 cagatgccac ttaataccgc gtgaatttcc caccgacccc tcacaaacaa tggcctcagg
    18961 ccatgcatca atcccgccag cacagcgtac agcagtattg accatccgag tgaaatttca
    19021 accttgttgc cgattacctg caccgttagc gcgtcgagga gaatgccggc gtatagcgcc
    19081 accgccagca ttagcggcac agggagcgcc acggccgcat aaaaaacaat cagcggcacg
    19141 aggtaaatgt gcgtgttgta caaccaagcc agcggcggaa tgaaaaactc tccgatctgc
    19201 gcaatcgtca cgagcacaag caggagaagg aaaaaaatca tttgcgaccc gtgacgatga
    19261 acacgtcctc caggtgcgac aaatcgaccg ccggcgtgag ctgcgcctga ccgtccagct
    19321 cgcgcacgcg aaaacttttt acgacgccca ccggcagacc tgaggggaac actccgccca
    19381 cgccggatgt gtagaccttt tgccccggtt tgagatcggc ctgttttgaa agaaaattca
    19441 aatagagcga cggcgtcaaa ccgctggtca cccgttcacc tgacacgatc ccctgctccc
    19501 gcgtgccttc gaccgttgcc gcgactttgc agttttcatc cgagaccaaa agtacaaccg
    19561 aaatactcga ggcgaccgtc gtcgtcttgc ccaccaaacc ttcgtcggta acgaccgcca
    19621 tgtcgccttc gatcccgtct tctctaccgc gattcacggt gactgtgtgc caccaggttg
    19681 acgaatcgcg cgtcactacg acagccggaa cgagcttgaa tacggagcgc tcgcgatagt
    19741 tcaattgatg gcgcaacgag ttcacctcgc gctcgacatc gcgcaacgcc tggttcgtcg
    19801 cccgcagctc gcggttctcg acccggagct gcgcactctc gcgctccagt tgctcgagcg
    19861 tctttagacc ggtgcgaacc gaagtgattt gcttttgtaa acccgagccg ctgctcaaaa
    19921 acggtgcaat caactgataa atcccagcct gaaacttgcg cgtgctctcg gctccgaagc
    19981 tcaagaagta cccgaggatc accccaaaga tgagcagcgc gataaagttc gtgcgactca
    20041 ttgagactgc aggttgcaga tggcggatcg ccgatttcgc aagatcgaca tcaccggccc
    20101 ggcagaagcc tttgcgctat cccgtttccg agatccgcaa ttgtcaccgc cactggcgat
    20161 ctgccgaggc cacctgccgc aaaaatttaa gttcattgag gcatttgccc gtgccctccg
    20221 cgaccgcgct caagggatct tccgcaacgt ggacgggcaa accagtctcc tcgctcaaga
    20281 gcttgtcgaa gccacgcaga agcgcgccac cgccagcgag cacaagtccg cggtccacca
    20341 gatcggccga aagctccggc ggacaacgct ccagggtaat gcggactgaa tcgacaattg
    20401 tcgagatcgg ctcgagcaac gcttcgcgca cctcctgcga tgtgatcatc agcgtctttg
    20461 gcaaaccagc caccaggtcg cgccctttga cttcgacact ggtctccttc tcggttggat
    20521 atgcagagcc gattttgatt ttgatttctt ccgcggtgcg ctcgccgacc atcaggttgt
    20581 aagcccgctt catgtattgc acgatcgctt catcgagctc atcgcccgca acgcgtacgc
    20641 tgcggctaaa cacgatccca gagagcgaga tcagtgcgac ttcggtcgtg ccgccaccga
    20701 tgtcgataat catattaccg gctggatcct gcaccggcag ccccacaccg atcgccgccg
    20761 ccatcggctc ttcgatcaag tacacctcgc gcgctccggc gtgcgtcgcg ctttcgcgca
    20821 ccgcgcgctt ttccacctcc gtgatgcctg atggcacggc aatcaccact cgcggccgcg
    20881 ccttcacgcg acgattgtgc accttcgaaa tgaagtgccg cagcatcgcc tccgtcactt
    20941 cgaagtctgc gatcacacca tctttcaagg ggcggacggc gacgatgttc gacggcgtgc
    21001 ggccgagcat ccgcttggct tcgtcgccga ccgccagcac tttgttcgtg ccggcttgca
    21061 ccgctaccac cgatggctca cgcaaaacga tcccttggtc tttgacgtaa acgagggtgt
    21121 tggccgtgcc gagatcgatc ccgatgtcgt tcgacaacca gccgaaaagt ccttcaaaca
    21181 tgtattacct cattcgcgca cgctcagtcg ttaggaaacg ctcttgtcga ttttgcctga
    21241 agcaaagtct gcgccgcgaa cgcattcgaa ccgcctgaaa cgcggttaag atgaaacaga
    21301 gcctcacatc gtcaagggta tttccgttgc agtgctcgag tactcgcgtg ctggagtaat
    21361 ggttgccaac gccgcatcac tccattactc catcactcca tgtcagtgct tgtcgctccc
    21421 tccattctcg ccgctgactt ctcgcggctc ggcgacgaag ttcgcaacgt cgaacgagcc
    21481 ggcgccgatt ggatccactg cgacatcatg gatggtcatt tcgtcgacaa catctcgttc
    21541 ggcccgggga ttgttaatct tgtccgcaac caaaccaaac tgccgctcga cgtccatttg
    21601 atgatcgaac acgccgatca ttacgtgccg cgatttgtcg cggcaggcgc gaactcaatt
    21661 acggttcacg tcgagccgga agcgaaacac gatgtcgaga aaacgttggc gcagattcgc
    21721 gctgccggct gccgcgtcgg gctgacgcta aatcccgaga cctcattcga tttagtcgag
    21781 ccgtatctcg aaaggatcga tatgctgctg atcatgaccg tccatccggg tttcggtggc
    21841 caatcattcc gtgccgatca aatggcgaaa gtgaaacgcg cgaaatcgct taacgacaag
    21901 atcaacattg aagtcgacgg cggcatcaac gccgaaaccg cgcggctctc aattcaaaac
    21961 ggcgcgaacg ttcttgtcgc cggcacgtcc attttccaca cgcaagatta cgcaaaagcc
    22021 atccgcgagc tacgcggcgc ctaggacgcc gtcatcccga gccgcgcaga cgaccgcgct
    22081 gtggagattt ggtgccgcta cgatatggag gttcgccaac taactgctta cccaagtcgc
    22141 gccacttcgg attgagttta cgcaccaact cgttcttctt ctcgcggcac caacccctaa
    22201 tttccttctc gcgtgagatg gcgtcgtgtg cgcgatcaaa tgcttcgtag tacacgagcc
    22261 ggtccaactt gtaacatttg acgaagtgtt tttgtccgtg ttgtcgtgaa atcagaggcc
    22321 gttccaaact gttcgtcacc ccggtgtaca gaactactcg gctccgattc gtcagcatgt
    22381 agacgaaata cttgttcagg acgcccgcca ccgagctgtg atcctgaacg aagtgaagga
    22441 cctcacacag gcccattgag cactggaaat accgtgtgat ccacagcgtg atccgagatc
    22501 cctacgccgt ctgcgcggct cgggatgaca gccgccatgc accatggggt catcctgagc
    22561 gaagcgaagg accgcgccta tttagacagc gtcacgcgat gcaactcgtg tgatcaacca
    22621 cccattatga cgcgcttagt ctgcgcgatt cgagatgaca ctggcgttag ctcgcgccga
    22681 t
//
LOCUS       DASTDG010000047         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1353016, whole genome shotgun sequence.
ACCESSION   DASTDG010000047 DASTDG010000000
VERSION     DASTDG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353016"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="ccsA"
                     /locus_tag="VFO30_01520"
     CDS             <1..448
                     /gene="ccsA"
                     /locus_tag="VFO30_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9417994.1"
                     /translation="ARADGAMHFWLYRVLQLGVLLLAAGTILGGVWANYSWGRFWGWD
                     PKETWALIALLCYILTLHGRLAGWWTEFGLVVASVVCFLAVLMAWYGVNFVLGKGLHS
                     YGFGIGGETYVAGFIIVDLAFVGFAVWRYRTSIRARSAVEAVVSAA"
     gene            complement(887..1816)
                     /locus_tag="VFO30_01525"
     CDS             complement(887..1816)
                     /locus_tag="VFO30_01525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9417995.1"
                     /translation="MNIGFVGVGRMGANMARRLKDRSAKGRIRRGEQFHVTAVYDTNR
                     AAATALVSELGCAACQHLSEVTAESDVIFTVVTDDAAMRQIFSGSGDNLLVNARDKLF
                     VNCATISPQVHVDLEQLAAKAGAQSLEACMASSITQAREGKLYLMCGGSEEAFRKAEP
                     ILKELGSTVRFIGKAGEAAKVKALVNMVMNINTAGLAEGLALGHALGLDLTMLREVFS
                     QTGANSRVLETDGEDIQNREHSCFFSAAHAAKDSGIAVELARRVGLDLPLARATKEQY
                     ERMIAEGLGELDKSGIAELTFKDRSASRQKAAE"
     gene            complement(1816..2199)
                     /locus_tag="VFO30_01530"
     CDS             complement(1816..2199)
                     /locus_tag="VFO30_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9417996.1"
                     /translation="MDKAELLTRTKLFGLRVLKLVGHLPQTTAGRAISNQLVRSGTAI
                     GANYRAARRSRSRAEFAAKLGVVAEEADETLYWLELIGDGDLLPAKKLDDILKEAHEL
                     TAIFTAGRRTSVRHQTSNIKRQTSS"
     gene            complement(2229..3218)
                     /locus_tag="VFO30_01535"
     CDS             complement(2229..3218)
                     /locus_tag="VFO30_01535"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9417997.1"
                     /translation="MKSPMKVAVTGAAGQIGYSLVFRIASGEMFGADQPISLHLIEIP
                     AALGALEGVVMELHDCAFPILQTVVPTADLDEGFRDINWALLVGSVPRKAGMERKDLL
                     GINGKIFVGQGEALERNAAADVRVLVIGNPCNTNCLIAMNNAREIPHERWFAMTRLDE
                     NRARAQLAHKAGVEIARVTNMTIWGNHSSTQYPDFYNAKIDNRAANEVIGDENWLKNE
                     FIATVQQRGAAIIKARGLSSAGSAANAIVDTVHFLTNNTPRDDWHSVAICSDGSYGVE
                     QGLISSFPVRVQGESCEIVQDLRLNDFSRNKIDTSVAELNEERSMVGELLGNV"
     gene            complement(3218..>4205)
                     /locus_tag="VFO30_01540"
     CDS             complement(3218..>4205)
                     /locus_tag="VFO30_01540"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9417998.1"
                     /translation="GAPPETHFKDDEFTQKWGQNWGIPLYRWDVMRSRNFEWWRQRVR
                     GVRHSFHLFRIDHVLGFYRIYAFPWRPRENREFLRLNWNQMLERTGGRAPHFAPRDDV
                     TPENCDANRRDGTEYLRVVLQESAGAPVIGEDLGTVPDYVRPSLRSLGIAGFKIPQWE
                     YYNSRVTPGADYERLSVATYATHDHPPVRALWEEAREKNPEPDSDLMKIATFAGFDTK
                     IGNLDYERDFYPAIMDALFKCESWMTVVMITDLLARKYRFNVPGVAGGANWTRRMQRA
                     VSQLRSSPRERRRMKVIRELLEKSGRIDPQISQITSSAKNLCESVKSVDKRS"
BASE COUNT          795 a         1247 c         1222 g          941 t
ORIGIN      
        1 ggcgcgcgcc gatggtgcga tgcatttctg gttgtaccgc gttttacagc ttggcgtgct
       61 cctactcgcc gccggcacga tcctgggcgg agtctgggcg aattattcct ggggccgatt
      121 ttggggttgg gacccgaagg aaacctgggc gctgatcgcg ctgctttgtt acatcctcac
      181 cttgcacggt cggctcgccg gttggtggac tgagtttggc ctcgtcgtgg caagcgtagt
      241 ctgcttcctt gcggtgttga tggcttggta tggcgtcaat ttcgtgctcg gcaaaggtct
      301 ccacagttac gggttcggta tcggcggcga gacctatgtg gctggcttta ttattgtcga
      361 tcttgcattc gtcgggttcg cggtgtggcg ttaccggacg agtatacgtg cccgcagtgc
      421 tgtagaggcc gtcgtctcgg cggcctgatc tagctgcgct caacacgtgg cgcacgtgcg
      481 aaccagcagt atgccaggaa aaaagacaat gcatccgcgc cgctcactcc ggccgcaatc
      541 gccgcctgcg cgtcccggtg aaaacatcca gatcggtgga acgaaaatcg gggttcgaag
      601 cggcggccaa aaatccgcaa cgactcgcgc caaacgccgc tcagccggcg tgcgctaata
      661 ttcgggcaaa cgaagcaccc tcaccttaat cctctccctc agggaaagga ggacgcgaca
      721 gcgccaggtg agggcagccc tgatgactcg ctcttcgcgt taggaccggt cgccggactg
      781 tttatgtcgc cgaacccggc caagccgggg cagctccttc tcatttaggc gttgaatttt
      841 acctcagcgt tgaaaagaag gagctcgccg cggcgagctg attgtttcac tcggctgcct
      901 tctggcgcga agcggatcga tctttgaagg tcagctcggc gattcctgat ttgtcgagct
      961 cgccaagacc ttcggcgatc attcgctcgt attgctcctt cgtcgcacgc gcgagcggga
     1021 gatcgagccc gacgcgacgc gcgagttcga cggcgatgcc gctgtctttc gccgcgtgcg
     1081 ccgcggagaa aaaacaactg tgttcgcggt tttgtatgtc ttcaccatcg gtctcgagca
     1141 cgcgtgagtt cgccccggtt tgcgagaaaa cctcgcgcag catcgttaaa tccagcccga
     1201 gtgcgtgacc aagcgccagt ccctcggcca ggccggcggt attaatgttc atgaccatgt
     1261 tgacgagtgc tttgactttg gccgcttctc cggctttgcc gatgaaccgg accgttgagc
     1321 cgagttcttt cagaattggc tcggctttac gaaatgcctc ctcgctgccg ccgcacatga
     1381 ggtaaagctt gccctcgcgc gcctgcgtga tgctcgaagc catgcaggct tcgagacttt
     1441 gcgcacccgc tttggcggcg agctgttcaa gatcgacatg cacctgcggc gaaatcgtcg
     1501 cgcaattgac gaacaatttg tcgcgcgcgt ttacgaggag gttgtcgccg ctgccgctga
     1561 aaatctgccg catcgccgcg tcatcggtga cgaccgtgaa gataacgtct gactccgcgg
     1621 tgacttccga cagatgctgg caagcggcgc agccaagttc ggataccaac gcagtcgcgg
     1681 ccgcacggtt cgtgtcgtaa accgcggtga catggaattg ttcgccacgg cgaatccgtc
     1741 ctttggcgga tcgatctttc agtcggcgcg ccatgttcgc gcccattcga cctacgccaa
     1801 caaatccgat gttcatcagg aggatgtttg acgtttgatg tttgacgttt gatgtcgcac
     1861 cgaagtgcgt cgaccggcgg tgaaaattgc ggttagctcg tgtgcttctt tcaagatgtc
     1921 atcgagcttt ttggcaggaa gcagatcgcc gtcaccgatc aattcgagcc aatacagcgt
     1981 ctcgtcagct tcttcggcga ctactccgag ctttgctgca aactcggcgc gcgatcgtga
     2041 gcggcgggcc gcgcgataat tggcgccaat tgcagtgccg ctgcgaacaa gctgattgct
     2101 gattgcacga ccggctgtcg tttgtggcaa atgacccacg agcttcagga cacgcagtcc
     2161 gaacaatttc gtgcgggtga gcaactccgc tttgtccacg cgctcgacct tacatcaaac
     2221 atcaaacctc aaacattccc cagcagttcg ccgaccattg accgctcctc atttagctcg
     2281 gcgaccgatg tgtcgatttt gttacggctg aagtcgttca agcgcaggtc ctgcacgatt
     2341 tcgcagcttt cgccctgcac gcgcaccggg aaggaggaga tcagtccttg ctcgacgccg
     2401 tagctgccat ccgagcagat ggcgacgctg tgccaatcat cgcgcggtgt gttgttcgtg
     2461 agaaaatgca ccgtatcaac aatcgcgttt gcggctgaac cggcggagga aaggccgcgc
     2521 gctttgatga tcgcggcgcc gcgttgttgc acggtcgcga tgaactcgtt cttgagccag
     2581 ttttcgtcgc cgatcacttc gttggccgct cgattgtcga tcttcgcgtt gtaaaagtcc
     2641 ggatactggg tcgacgaatg gttcccccag atcgtcatgt tcgtgacgcg cgcgatctcc
     2701 acccccgctt tgtgcgcgag ctgggcgcgc gcgcggtttt catccaaccg ggtcatggca
     2761 aaccagcgtt cgtgggggat ctcgcgcgcg ttgttcatcg cgatcaggca attggtattg
     2821 cacggattgc cgatgacgag cacccgcacg tcggccgcgg cattcctttc taaagcctca
     2881 ccttgcccga caaaaatctt cccgttgatc cctaacaaat ccttgcgttc cattccggct
     2941 ttccgcggca cgctgccgac gagcaacgcc cagttaatgt cgcgaaagcc ttcgtccaaa
     3001 tcagccgtgg gcacgacggt ctgcaaaatc ggaaatgcac agtcgtgcag ttccatcacc
     3061 acgccctcga gcgcgccgag cgcggccgga atctcgatga ggtgcaagct gatgggctga
     3121 tcggcgccga acatttcgcc ggaagcaatc cggaacacga gggagtagcc gatctgcccg
     3181 gccgcgccgg taaccgcgac tttcatcgga gacttcatca gcttctctta tccacagatt
     3241 tcacggattc acacagattt tttgcggaag aggtaatctg cgaaatctgt ggatcaattc
     3301 tgccgctttt ctcgagcaac tcgcgaatga ctttcatccg ccgtcgctct ctcgggctgg
     3361 agcgtaattg cgacaccgcc cgctgcatgc ggcgggtcca attcgcgccg ccggccacgc
     3421 cgggaacgtt gaagcggtat ttgcgcgcga gcagatcggt gatcatcacc acagtcatcc
     3481 acgactcgca cttgaacagc gcgtccatga tcgccgggta aaaatctctt tcgtaatcga
     3541 gattaccgat cttggtgtcg aacccggcga aggttgcgat tttcatcaag tcggagtccg
     3601 gctccggatt tttttcgcgg gcctcttccc ataatgcgcg gaccggcggg tgatcatgcg
     3661 tagcataagt ggcgaccgac aaccgctcat aatcggctcc cggcgttacc cggctgttgt
     3721 aatactccca ctgcggaatt ttgaagccag ctatgcctaa cgagcgcaag ctcgggcgga
     3781 cgtaatcagg aactgtcccg agatcttcac cgatcacggg agcgccggcc gattcttgta
     3841 aaacaacgcg cagatattcc gtgccgtcgc gccggttcgc atcgcagttt tccggcgtga
     3901 cgtcatcgcg cggcgcgaaa tgtggggcgc ggccgccggt tcgctcaagc atctgattcc
     3961 aattcagtcg gaggaactcg cgattttccc gcggtcgcca cggaaaggcg taaatgcggt
     4021 agaagccgag cacgtggtcg atgcggaaaa ggtgaaagct atgccgcact ccgcgaacgc
     4081 gttgtcgcca ccattcgaaa ttgcgcgacc gcatcacgtc ccagcggtag agcgggatgc
     4141 cccagttctg tccccatttt tgcgtgaact cgtcgtcttt gaaatgcgtt tccggcggcg
     4201 cgccg
//
LOCUS       DASTDG010000048         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_682122, whole genome shotgun sequence.
ACCESSION   DASTDG010000048 DASTDG010000000
VERSION     DASTDG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682122"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFO30_01545"
     CDS             complement(<1..1063)
                     /locus_tag="VFO30_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9417999.1"
                     /translation="MNRKRFLDFARNDKIGYTQVGGSNLTITFVRRGYSVSGGAEAYL
                     KRLAHGMVNLRHNIELITSEDWPKSEWPSGQITRLRARSAADFADQVEQERAQRAGTL
                     LFSLERIWRCDIFRAGDGVHRAWLNRRKKFELPLQRFARGLSSKHQQLLQLEAALFAK
                     GGAARTIVASEMVKNEIVDLYGYSADKIDIIRNGVPVERFRFDPELRERSRANLKLQR
                     DHIALLFVGSGWERKGLLFAMEAMAMCRDRRMRLLVAGRGKTQRYYKSKHVRFLGEVV
                     DLLPIYAAADIFILPTIYDPFSNACLEALACGLPVITTRSNGFSEIIDHQVHGSIVDA
                     PNDITALADMLKFWSDETRR"
     gene            1138..2334
                     /locus_tag="VFO30_01550"
     CDS             1138..2334
                     /locus_tag="VFO30_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9418000.1"
                     /translation="MTGRRSGEKNPRLLPERLLYSIFARIGGSGLDTDAFEALRGSYR
                     GGFLGKAIAYDNRQKEIPRALIHSLRWHPVRLLSFLDRPYYYGAKKKYLDWIASRHLA
                     RGGYDFFHSWSGDCLLSLRETKRRRIPSLIEIPTWHRDRTKVKPMEPLGEESETWWWR
                     KGKAGLLLERKRFIEEYQLADVLVVLSEIAAETFRVQGFPEDKLFYLPRGVDVERFKP
                     RPRPQIFRTIFSGALIERKGIHHLLEAWHRLNLKDAELWLVGAIHDEAKPHLKQFWRE
                     NIRTFGFVREPEKYLSQSTIHLFPSQWEGSAKVTYEAAACGLPQITTREAGDVVRDQI
                     EGIIVRPGDVNAIAAGIEYFYRHPEAVEKMGRAARQRVVENFTWDHYRTRLLDAYETA
                     MRKVAC"
     gene            2349..3368
                     /gene="rfaQ"
                     /locus_tag="VFO30_01555"
     CDS             2349..3368
                     /gene="rfaQ"
                     /locus_tag="VFO30_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HET9418001.1"
                     /translation="MKILLLQLKRIGDLILTLPAIEALREKFPDAEITLVVAKECAEL
                     LPAMSTVDHAHVARRNLRDISLFFTIARQKFDCCIDFTQNDRSALLTFLSGAPKRIGA
                     SRVQLQSGRRARVYNEFAHLRLKEMHTIDYNLSLLEPLGIRNARPDLRLQLPESARTK
                     ARELLRAAKIDNHFVVLHPGSARVEKFWEAERWAAVITHAQTQLQTAVVITGGTWRFE
                     QEHIQQIKERAGNAIVDLSGKTDLLTLAAVINEARLLVTVDSAAMHLAATQRTPQVIL
                     FGPTNPFHWRPRETAALILQGESGAPVTTFVPKQPRASMRQISTEAVINGMDALLSPA
                     AARTS"
     gene            3365..>3984
                     /locus_tag="VFO30_01560"
     CDS             3365..>3984
                     /locus_tag="VFO30_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9418002.1"
                     /translation="MSEQQSEKQKLSFWQTVRQAWGPYRRIYSYVKPYRLRFVLGLTF
                     GFAFGAVNSLFPLVVARVTSVIFHGAAPNPMALRSNLQLLNTGPRINSIVLTCLAIPA
                     IMTVRSLCSYANAYYMQWVSNKVVTDIRTQLFQKILRHSMDFFNKMRGGFLISRITND
                     TRAMQMALSTVSSDVFKQPVTIIGGIAVLLLMDWKFTVVTLVLFPT"
BASE COUNT          840 a         1110 c         1187 g          847 t
ORIGIN      
        1 tgcggcgcgt ttcgtccgac cagaatttca gcatgtccgc caatgcggtg atgtcgtttg
       61 gagcatcgac gattgagccg tggacttggt ggtcaatgat ttcgctgaag ccgttggagc
      121 gagttgtgat taccggaagg ccacaggcga gcgcttcgag gcaggcgtta gagaacggat
      181 cgtagatcgt ggggagaatg aaaatatcgg cggcggcgta gatcgggaga agatcgacaa
      241 cttcgccgag aaatcggacg tgttttgatt tgtaataacg ctgcgtcttc ccccgtccgg
      301 caacgagcag acgcattctg cggtcgcgac acatagccat ggcttccatg gcgaagagca
      361 agcctttgcg ttcccagccg gagccgacga agagaagggc aatgtggtcg cgctgtagtt
      421 ttaaattggc gcgcgacctt tcccgaagct ctggatcgaa ccggaatcgc tcgaccggca
      481 ctccgttacg gatgatgtcg atcttgtcgg ccgagtagcc gtaaagatcg acgatctcgt
      541 tcttcaccat ttccgatgcg acaatggtgc gggcggcgcc ccctttggcg aagagcgcgg
      601 cttcaagttg gaggagttgc tgatgcttcg agctcaggcc tcgcgcgaat ctttgtagcg
      661 gcagctcgaa tttttttctg cgattgagcc aggcgcgatg gacgccatcg ccggcacgaa
      721 aaatatcgca gcgccaaatg cgctccagac tgaacaagag cgtcccagcg cgctgtgcgc
      781 gttcctgttc gacctggtca gcgaagtcgg cggccgatct tgcgcgcaat cgcgtgattt
      841 ggccggacgg ccactcactt ttcggccaat cctcgctcgt gattaattca atgttgtggc
      901 gaagattgac catcccatga gcgagacgtt tcaaataggc ctcggcgccg ccgctgaccg
      961 aatagcctcg gcgcacgaag gtgattgtaa gattgctgcc tcccacttgc gtgtaaccaa
     1021 tcttgtcatt ccgagcgaag tcgaggaatc tcttacggtt cacgcacccc cgcgatgctg
     1081 aagatgagag atgtctcgct ccattcgact aacactcagg acaggcttgc gctggacatg
     1141 acaggacgcc gtagcggcga gaaaaatccg cgcctgcttc ccgagcgctt gctctactcg
     1201 atcttcgcgc gcatcggcgg atcgggcttg gacacggatg cgttcgaagc tctgcgcggt
     1261 tcatatcgcg gtggatttct cggcaaagca atcgcctacg acaaccggca gaaggaaatc
     1321 ccgcgggcat tgattcattc gctccgctgg catccggtgc gactgctttc gtttctggat
     1381 cggccttatt actacggtgc gaagaaaaaa tacctcgact ggattgcatc acgccatctg
     1441 gcgcgaggcg gctacgattt tttccacagt tggtcgggcg actgcctgct gtcgttacgc
     1501 gagacaaagc ggcgacgaat tccgtcgtta atcgagatac cgacgtggca tcgcgaccgc
     1561 acgaaagtga aaccgatgga accgctcggt gaagaatcgg agacgtggtg gtggcgcaaa
     1621 ggtaaggcgg gtctgttgct cgaacgaaaa cgcttcatcg aggaatacca gctcgccgat
     1681 gtgctcgtcg tcctgtcgga aatagcagcg gagacgttcc gtgtgcaagg ctttccggaa
     1741 gataagttgt tttacctgcc gcggggagtg gatgtcgagc ggttcaagcc gcggccgcgg
     1801 ccgcagatct ttcggacaat cttttccggc gcgctgatcg agcgcaaagg cattcaccat
     1861 ctgctcgaag cctggcatcg gctgaacctg aaagacgcgg agttatggct cgtgggcgcg
     1921 attcacgacg aagcaaaacc gcacctcaaa caattctggc gcgagaacat ccgcactttc
     1981 ggctttgtgc gggagccgga gaaatatctg agccaaagca cgattcacct cttcccctcg
     2041 caatgggaag ggagcgcgaa agtcacgtac gaagcggcgg cgtgcgggct cccgcagatt
     2101 acgacgcgtg aggcgggcga cgttgtccgc gaccaaatcg aaggaatcat tgtccgcccc
     2161 ggcgacgtga acgcgatcgc agcagggatc gagtactttt accggcatcc cgaagcagtc
     2221 gaaaagatgg gtcgcgcggc gcgtcagcgg gtggtggaaa acttcacgtg ggaccattat
     2281 cggacgcgct tactcgacgc ctacgaaaca gcgatgcgca aagtggcgtg ctaatcgcga
     2341 ccgcgtgcgt gaagattctg ctgctacaac ttaagcgcat tggcgatctg attctgacgc
     2401 tgccagcaat cgaagcgctg cgggaaaaat ttcccgatgc cgagatcacg cttgtggtgg
     2461 caaaggagtg cgcggaattg ctgccggcga tgtcaactgt cgatcatgcg catgttgcgc
     2521 gtcgtaatct gcgcgacatc agcctattct ttacgattgc gcggcagaag ttcgactgct
     2581 gcatcgattt tacgcagaac gatcgatccg cgctgctgac gtttctttcc ggcgcgccga
     2641 aacgcatcgg cgcatcgcga gtgcagctgc aatcgggacg ccgcgcgcgc gtttacaacg
     2701 agttcgcgca tctgcgactg aaggagatgc acactattga ttataatctc tcgctgcttg
     2761 agccgctcgg gatcaggaat gcgcgcccgg acctgcgatt gcagctgccg gagagtgcgc
     2821 gcacaaaagc acgtgagctt ttacgcgctg ctaagatcga caatcatttt gtcgtgctgc
     2881 atccaggctc ggcccgcgtg gagaagtttt gggaagcaga gcgctgggcc gcagtcatca
     2941 cacatgcgca aacccaactg caaaccgcgg tcgtgatcac gggtgggacg tggcgctttg
     3001 agcaggaaca tattcagcag atcaaagagc gcgcgggcaa cgcaattgtc gatctttcgg
     3061 gcaaaaccga tttgctgaca ctcgcggcag tcattaacga ggcgcggctt ctcgtcacgg
     3121 tcgattcggc ggcgatgcat ctcgccgcca cgcaacgaac accccaggtc attttgtttg
     3181 gaccaacgaa tccgtttcac tggcgaccac gggaaactgc cgcgctgatt ttgcagggcg
     3241 agtcgggcgc gccggtcacg acttttgtcc cgaaacagcc gcgcgcgtcg atgaggcaaa
     3301 tctcgacgga ggcggtgatt aatggtatgg atgcgctgct atccccggcg gctgcgcgaa
     3361 cttcatgagc gagcaacaat ccgaaaagca gaagctgtcg ttttggcaaa ccgtccgcca
     3421 ggcgtggggc ccatatcggc gcatctacag ttatgtgaaa ccgtaccgcc tgcggtttgt
     3481 ccttgggctc actttcgggt tcgccttcgg cgcggtcaat tcgcttttcc cgctggtcgt
     3541 tgcgcgggtg acgagcgtca tcttccacgg cgcggcgccg aacccgatgg cgttgcgctc
     3601 gaacctgcaa ctgctcaaca ccgggccgag gatcaactcg attgtcctga cctgtctcgc
     3661 cattccggcg atcatgacgg tgcgcagtct ctgctcctac gccaacgcct attacatgca
     3721 gtgggtgagc aacaaggtcg tgaccgacat tcgcactcag cttttccaaa aaattctccg
     3781 gcactcgatg gatttcttta acaaaatgcg tggcggcttc ctgatttcgc ggatcacgaa
     3841 cgacacgcgc gcgatgcaaa tggcgctgag cacagtgagc agcgacgtct tcaaacagcc
     3901 agtcactatc atcggcggta ttgctgttct tctcttgatg gactggaaat tcacagtcgt
     3961 gacgctggtc ttgttcccga cgtg
//
LOCUS       DASTDG010000049         4209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1470175, whole genome shotgun sequence.
ACCESSION   DASTDG010000049 DASTDG010000000
VERSION     DASTDG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470175"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFO30_01565"
     CDS             complement(<1..704)
                     /locus_tag="VFO30_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9418003.1"
                     /translation="MMQPLPGFRDFLPNDCARRNYIFAKWREVARRYGFVEWDGPILE
                     PTDLYRKKSGAEIVDQLFNFTDKGEREVAMRPELTPSLARVVAAHEREFKKPLKWFSI
                     GQFFRYEKQQRGRLREHFQLNCDIIGEDGAEADVELTALCIDLLRAFGFGSSDVVVRI
                     SDREFWTDFLNRKNVPAAQIDEALQAIDKFGREHRDQTAAKLGPLADSVLKILNDGGT
                     SEKLERLVAGLRERHLA"
     gene            835..1257
                     /locus_tag="VFO30_01570"
     CDS             835..1257
                     /locus_tag="VFO30_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418004.1"
                     /translation="MKETTLVKLLVGAACAIALGVAVAQESSTTTSTTNAATGTTEST
                     TTTTSSAGTITAYTPGSDYITFRTTTDAAPVKYYYTKETTILDPAGRTVTWSDVRPDL
                     PATVYYVREGDRMIVRKIVLSKPVKVEKETTTTTTTQP"
     gene            complement(1365..2336)
                     /gene="galE"
                     /locus_tag="VFO30_01575"
     CDS             complement(1365..2336)
                     /gene="galE"
                     /locus_tag="VFO30_01575"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET9418005.1"
                     /translation="MKILVTGGAGYIGSICAELLLNEGHEVTIADNLSEGHRRAVDAR
                     ARFIEADLQDRQRTLSILRETKPEAVMHFAASALVAESMRDPSKYFRSNIGNGINLLD
                     AIIAVGCGRIIFSSTCAIFGPPERLPLDEMLPQRPINPYGESKLVFEKILRWYREIHG
                     LNSVSLRYFNAAGASEKFGEDHREETHLIPNVLKVALGQKPRVEVFGTDYDTPDGTCI
                     RDYIHIIDLARAHILALQSAKSEFYNLGTGGGSSVREVINCCRKITGRKIDIVEKPRR
                     PGDPPRLIASSDKIKRELGWKPQFQSLEAIVESAWKWHQRFPRGYEG"
     gene            complement(2368..2739)
                     /locus_tag="VFO30_01580"
     CDS             complement(2368..2739)
                     /locus_tag="VFO30_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET9418006.1"
                     /translation="MSEGVGRRNALGVSLSGRHVMSAASDWSRYPLGTRFRIVGTQEE
                     YIIDDYGTALVGTNTIDLYKPTRLAMRRWGVRHIDIDVLQWGSTEQSLRVLRPRQKHS
                     AARRMILALEKKTASGPPRLD"
     gene            complement(2987..3472)
                     /gene="mscL"
                     /locus_tag="VFO30_01585"
     CDS             complement(2987..3472)
                     /gene="mscL"
                     /locus_tag="VFO30_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET9418007.1"
                     /translation="MNWFKHVWKEFKEFAIKGNAVDLAVGVIIGAAFGAIVNSLVKDM
                     IMPPISVLSGGLDFSNKFIVLRAAKGGSVNFNTPADALKAGAITWNYGNFITLIINFL
                     IVAAAIFLLVRGINRLKRPSPHAEPVTKDCPACAMTIPIKATRCPHCTTELPGSPCLT
                     A"
     gene            complement(3469..>4209)
                     /locus_tag="VFO30_01590"
     CDS             complement(3469..>4209)
                     /locus_tag="VFO30_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9418008.1"
                     /translation="LVNQGIILGEDNQKMSKSRGNIVNPDDVIAQYGADAFRCYEMFM
                     GPLEQMKPWSMRGVEGISRFLARVWRLMMTENQAGEWELSSALQDVDLTRDQQRVLHG
                     TIKKVTDDIESLSFNTAISQMMIFVNAFTSAEAIPIAAMRTFLVLLKPFAPHLSSELW
                     ERLNSPGAITDQPWPDYEERWLAEDEVEIVVQVNGKLRDRMKMSIFATDEEMKAAALT
                     APKIQRLISGKPVRKVVVVPKKLVNIVV"
BASE COUNT          893 a         1190 c         1221 g          905 t
ORIGIN      
        1 gccagatggc gttcgcgcag acctgcgacg agccgctcca gtttttcgct cgtgccgcca
       61 tcgtttaaaa ttttcaaaac ggagtcggcc agcggcccaa gtttcgcggc ggtctggtcg
      121 cgatgttcgc gtccgaactt atcgatcgct tggagtgctt cgtcgatttg cgcggcgggc
      181 acatttttcc gattgagaaa atcggtccag aattcgcggt cgctgatccg caccaccaca
      241 tcgctgctcc cgaagccaaa agcgcgcagc agatcaatgc agagcgctgt cagttcgaca
      301 tctgcttccg cgccgtcctc gccgatgatg tcgcagttga gctggaaatg ttcgcgtaag
      361 cggccgcgct gctgtttttc gtagcggaag aactggccga tcgaaaacca cttgagcggt
      421 ttcttgaatt cgcgctcgtg cgctgcaacc acacgcgcca gactcggcgt cagctcgggg
      481 cgcatggcca cttcgcgctc gcctttgtcg gtgaagttga agagttgatc aacaatttcc
      541 gcgccgctct ttttgcggta aagatcggtc ggctccagga tcgggccgtc ccactcgaca
      601 aacccgtaac ggcgcgcgac ttcgcgccat ttcgcgaaaa tgtaattgcg tcgtgcgcaa
      661 tcattcggaa gaaaatcgcg gaagccgggg agcggttgca tcatagtcat gccagcgggc
      721 gaccgcccat cgtcaatttt acgaaatagg tatggaattg aataaactcg ggctggaacg
      781 agtcggacat agctacagcg gagagcatcc gtttaaccaa ggaaaacgac aaacatgaaa
      841 gaaacaacat tagtgaaact gttggtcggc gccgcttgcg cgattgcgtt gggcgtggct
      901 gtcgcgcagg agtcgtcaac gacaacgtcg acgaccaacg ctgcgaccgg cacgacggag
      961 tcaaccacga cgaccaccag cagcgcaggg acaatcacgg cctacacgcc gggttcggat
     1021 tacattactt tccggactac gaccgatgca gcgcccgtga agtattacta caccaaggaa
     1081 acgaccatcc ttgatccggc aggtcgcacc gtcacctggt cggacgtgcg gcccgatttg
     1141 ccggccacag tctattatgt gcgcgaaggc gatcgcatga tcgtgcgcaa gattgtgctg
     1201 agcaaaccag tgaaggttga gaaagaaact accaccacaa ctacgacaca gccgtaaaca
     1261 gaggcgaatt gtgtgaattg aaagcggtcg ctgcggcgac cgctttttgt tttccgtagg
     1321 cgagtctctg gctcgcaggt cgcaggcgag acgctcgcgt tattttaacc ttcgtagccg
     1381 cgtgggaatc tctggtgcca tttccacgcg ctctcgacga tcgcctcaag cgattggaac
     1441 tgcggtttcc agccaagctc gcgtttgatt ttgtccgagg acgcgatcaa ccgcggcgga
     1501 tcgccgggac ggcgaggttt ttccacgatg tcgattttgc ggccggtgat tttgcgacag
     1561 caattgatca cttcgcgcac gctcgagccg ccgcctgttc ccagattgta aaactcactt
     1621 ttggcagatt gcagcgccag gatgtgcgcc cgtgcgagat cgatgatgtg aatgtaatca
     1681 cggatgcacg tgccgtccgg cgtgtcgtaa tcggtcccaa agacctcgac ccgcggtttt
     1741 tggccgagcg cgactttcag cacattcggg atcaaatgcg tttcctcacg atggtcttcg
     1801 ccgaatttct ccgacgcccc ggccgcgttg aaatagcgca gcgaaacgga attgaggccg
     1861 tgaatctcgc gataccaacg caggattttt tcgaacacca gcttggattc gccatacgga
     1921 ttgatcggcc gctgcggtaa catctcatcg agcggcaagc gttcgggtgg cccgaagatt
     1981 gcgcaggtgg aagagaagat gatgcgaccg caccccaccg cgatgattgc atcgagcagg
     2041 ttgatgccgt taccgatgtt gctgcgaaaa tatttggagg gatcgcgcat cgattcggcc
     2101 acgagtgcgc tcgcagcgaa atgcatcacc gcttccggtt tggtctcgcg caagatcgac
     2161 aatgtgcgtt ggcgatcttg caggtcggct tcgataaatc gcgcgcgagc atctaccgct
     2221 cgccgatgcc cctcactcag attgtcggcg atcgtgactt cgtgaccctc atttagcagc
     2281 agctccgcgc aaatgctgcc aatgtaaccg gcgccaccgg taacgaggat tttcatcgcc
     2341 gccaaagtcg atgccggcgc gcgtttatca atccaaacgt ggcgggccgg acgcggtctt
     2401 tttctccaga gcgaggatca ttcgccgggc agcggaatgt ttttgccgcg ggcgaagaac
     2461 tctcagactt tgctcggttg agccccactg cagaacgtcg atgtcgatgt ggcgcacgcc
     2521 ccatcggcgc atggcgagac gggtaggctt atacaggtca atggtgttgg tgccgacgag
     2581 ggcggtgcca taatcgtcaa tgatgtattc ctcctgagtg ccgacgatgc ggaagcgcgt
     2641 gccgagcgga tagcgcgacc aatcggacgc ggcgctcatc acgtgacggc ccgacaatga
     2701 caccccgagg gcattacgtc gcccaacgcc ctcgctcacg gtgtatgcag tcgtgcgcac
     2761 gtgatggcag cgggcgacag tcgcgaacgc gctgcgtgat cgctgcaaag ccgattgctg
     2821 cgcgcaaccg acggcaagtg agccaatcag cgcgagggtt ataaacttca gaggttttaa
     2881 cattagcaac accgcggccg agatagcagc actgcgccgc ctggcaagta attatttgcg
     2941 tatcaacgga ttcggacgcg atatccgaat gaatttgcag caactgctac gccgttagac
     3001 acggtgaccc gggaagctcg gtcgtgcagt gcggacaacg agtcgccttg attggaatcg
     3061 tcatcgcgca cgcgggacaa tccttcgtca ccggttcggc gtgcggtgac ggtcgcttca
     3121 accgattgat cccgcggacc aaaagaaaaa tggctgcagc cacgatgagg aaattgatga
     3181 tgagcgtaat gaagttcccg tagttccagg taatcgcgcc ggctttcaac gcgtcggccg
     3241 gcgtattgaa gttgaccgag ccgcctttgg ccgcgcgcag cacgatgaac ttgttcgaaa
     3301 aatcgagccc gccgctgagt acgctgatcg gcggcataat catgtctttg accagtgaat
     3361 tgacgattgc gccgaaggcc gcgccgatga tgacgccgac cgcgaggtcg accgcgttgc
     3421 ctttgatcgc gaattccttg aactctttcc agacatgttt gaaccagttc atacgacgat
     3481 gttgaccagt tttttcggga caacgacaac tttccggacg ggtttaccgg agatgagtcg
     3541 ttgaattttg ggcgcggtaa gcgcggcggc tttcatctct tcgtcggtcg cgaagatcga
     3601 catcttcatt cggtcgcgga gctttccgtt cacttgcaca acaatttcta cctcgtcttc
     3661 ggccaaccag cgctcctcgt agtccggcca gggctgatcg gtgatggccc ccggagagtt
     3721 caagcgttcc cacagctcgg agctgaggtg cggcgcgaat ggtttgagca gaaccaagaa
     3781 cgtgcgcatc gctgcgattg ggatggcctc ggcgctggtg aacgcgttga cgaaaatcat
     3841 catctgcgaa atcgcggtgt tgaacgagag cgactcgatg tcgtcggtca cttttttgat
     3901 cgtcccatgc aggacgcgct gctggtcacg tgtaagatcg acatcttgca gcgcggagga
     3961 caattcccat tcacccgcct ggttttccgt catcattaat cgccagacgc gcgcgagaaa
     4021 tcgagaaatt ccttcgacgc cgcgcatgct ccacggcttc atctgttcaa gcgggcccat
     4081 gaacatttcg taacagcgaa acgcatccgc gccgtactgc gcgatgacgt cgtccggatt
     4141 gacgatgttg ccacgcgact tcgacatttt ctggttgtcc tcgccgagga taatgccctg
     4201 attgacgag
//
LOCUS       DASTDG010000050         7077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_179247, whole genome shotgun sequence.
ACCESSION   DASTDG010000050 DASTDG010000000
VERSION     DASTDG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179247"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFO30_01595"
     CDS             complement(<1..326)
                     /locus_tag="VFO30_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418009.1"
                     /translation="MKRTIISFACALIAPLAFAQTSTTTTETTTSSPSTATGTTTTET
                     TKTYTSGTVTTYTPGKTVVVRSSEGPVTFALGTAANIGDAAGKVVTAPLRAGLKVRVY
                     YTGTGD"
     gene            complement(447..764)
                     /locus_tag="VFO30_01600"
     CDS             complement(447..764)
                     /locus_tag="VFO30_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418010.1"
                     /translation="MKRIIITAALALATPLIFAQTTTTTTESTTSTRGTVTTYEPGKT
                     IVVRTEKDENPISYVLGKTVHYVNKAGRTIDHHLIKPGTRVIVYSSKGRLHDAERVVV
                     DED"
     gene            872..3289
                     /gene="purL"
                     /locus_tag="VFO30_01605"
     CDS             872..3289
                     /gene="purL"
                     /locus_tag="VFO30_01605"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET9418011.1"
                     /translation="MSMSTAAADPLVTPQVVKEHGLTAEEFERIKKILGREPNFTELG
                     IFSVMWSEHCSYKNSRNELKKLPTTGPNILVKAGEENAGVVDIGDGWAIAFKIESHNH
                     PSAIEPFQGAATGVGGIIRDIFTMGARPEFCLNSLRFGPIVAQASGLQDHKRDARVTL
                     ANRRLFTGVVSGIAHYGNCVGIPTIGGEIYFDESFEGNPLVNVFCLGVLRHDQLARGT
                     ARGIGNPVFYVGAETGRDGLAGAAFASRELTEQSREDRPAVQVGDPFKEKLLLEACLE
                     LLAHDAVAGIQDMGAAGLTCSTCETASRAGSGIEIDLSKVPKREPGMTPYEILLSESQ
                     ERMLIIAKRGKENLVREIFEKWDVACAEIGRVTDDGIMRVRNNGSVAAEIPAKPLAEE
                     APLYSREAKEPVATRLWRVEGGRPAEPWLQNLQKIDNREALRQLLRDPTIASKNWVYR
                     QYDHTVRAGTIVKPGSDAAVFHVKYANKILAATTDCNSIYCALDPREGAKIAVAEAAR
                     NLTCAGAKPLAVTDNLNFGNPYKPENFWQLREAIEGIAEACRAFGTPVTGGNVSLYNE
                     SPAGVVDPTPTIAMVGLIDRPEHVTTQWFKKQGDAIILVGSGMGFQPMSHGQDADAAT
                     HLLGGSRYLKVCHGKKIGPPPHVDLTQEINVQSAVRELIRGGLVKSAHDCSEGGLAVA
                     LAECCFNPEKLLGAKVDLGDCSHGPAGRREETGHRPVATALFNESQSRIIISVAPESI
                     ERTIAMLRERGVPHEQFGTVGGEELRITVNGDSLAWPIVDLYDDWWNAIRRTVESDSA
                     AERIPSL"
     gene            3319..4818
                     /gene="purF"
                     /locus_tag="VFO30_01610"
     CDS             3319..4818
                     /gene="purF"
                     /locus_tag="VFO30_01610"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9418012.1"
                     /translation="MRLPPISTHQELPDRAALPQHECGLFGVFGHPKAAVLTYYGLFA
                     LQHRGQESAGIVTSSGPGTSFLIHKGMGLVSQVFGQAELEKLKGTRAIGHARYSTTGT
                     STIKNAQPFVVDCVRGQMAIAHNGNLTNADILRDELERRGSIFQTTADSEIILHLLAQ
                     PAGNGSNVLAALRAIKGAFSLLIMSERELIAVRDPFGWRPLSLGKIDGAYVLASETCA
                     FDLIHADFVREIRPGEVLIIDENGVRSEFPFPPEQPAFCMFEYVYFARPDSMIGGVNV
                     GKVRTEMGRELARRYPADADIVIPVPDSGNYAALGFAEELKIPFAHAFVRNHYIGRTF
                     LQPSQLIRDFDVRVKLNLIKEMVAGKRVVVVDDSIVRGTTARARVVNLREAGAKEVHM
                     RVSCPPHRFACHYGIDFPDPEKLIANKMSMEDICKYLGVDSLGYLDVEGMVRATGRKV
                     NEFCLACFNGHYPLPVDPALDKFIMEKREARSKALADQEAHPTLFADLK"
     gene            4910..5929
                     /gene="purM"
                     /locus_tag="VFO30_01615"
     CDS             4910..5929
                     /gene="purM"
                     /locus_tag="VFO30_01615"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9418013.1"
                     /translation="MTRKQKAYAHAGVDVDLANRLKRRIQRFVRRTHGPQVLGKIGGF
                     GGLFRAEFREMREPVLVSSIDGVGTKMKIAFALDRHDTVAMDLVNHCVNDIAVLGARP
                     LFFLDYIGGEKLEPRVFEQLLRGFARACHAAGCALIGGETAQMPDMYRRGEYDLVGCI
                     VGVVDHANIIDGRNIKPGDVVLGLASSGLHTNGYSLARKILFENMRLKPSSKIDDLRG
                     TVGDELLRVHKNYQPLLAKAPSGLIKGLAHITGGGLIDNLPRILPDDCDAIIETKSWR
                     VPLIFQILQENGNVDRTEMYQVFNMGIGMVAVVAERDANHMMSILRARRIGTIERGNR
                     KTQLL"
     gene            6009..6911
                     /locus_tag="VFO30_01620"
     CDS             6009..6911
                     /locus_tag="VFO30_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9418014.1"
                     /translation="MEKRRLGKTEMDVTVLGFGGSEIGYERVAQDAVERLLNSALDAG
                     LNVIDTAECYYGSEELIGRAVGKRRNEFYLFTKCGHPHGMESAANWSKVSILQSIERS
                     LRRLQTEVLDLVQLHSCSESELRNGEAIDASQTARERGLTRYIGYSGDSRAAHYAVEC
                     GAFDTLQTSINIADQEVIELTLPIARQKQMGVIAKRPIANATWKTGHRPPDSYHHEYW
                     ERLRRLNYDFLRTRPLEESISIALRFTLSVPGVHTAIVGTTNPERWQQNAKKLEAGAL
                     RDAEFNAIRHRWEEYAPRAWIGQI"
BASE COUNT         1501 a         2058 c         2074 g         1444 t
ORIGIN      
        1 ctatcccccg tgcccgtgta atacacgcgc acttttaggc cggcgcgcag tggagcggtc
       61 acgactttac ccgcggcgtc cccgatgttc gcagctgtgc caagagcaaa ggtcaccggt
      121 ccttcgctgc ttcggactac gacggtcttg cctggcgtat aggtcgtcac cgtacccgac
      181 gtgtaagttt tcgttgtctc ggtcgttgtc gtgccggtgg cggtactggg actcgaggtg
      241 gttgtttcgg tcgttgtcgt gcttgtttgc gcaaacgcca aaggcgcgat tagcgcacaa
      301 gcaaagctaa taatggttcg tttcatcttt gcctccaggt tgattaactc atgtgcgcgc
      361 tcgaatgagc gcgcgttatc tcattggact gctagacgcg gcgaattatt caacgccgcg
      421 cccaggttcc agccctcgta tcgctgtcag tcttcgtcca ccacgacacg ctcggcgtcg
      481 tgaagccgac ctttcgagct gtaaacaatg acccgtgtgc caggcttgat cagatggtga
      541 tcgatcgtcc tgcccgcttt gttcacataa tgaaccgtct tgccgagaac gtagctgatc
      601 gggttctcat ctttttcggt acgaacaacg atcgtttttc caggctcgta agtcgttacg
      661 gtacctctgg tgctggtcgt tgactccgtc gtggtggtgg tcgtttgcgc aaagattagc
      721 ggtgtggcca gcgccagcgc cgcagtgata ataattcgct tcatgtctgc tcctttcttt
      781 ttgatattcg acagcggccg gccagtaagt attcgaactt cagggtcgcc aactcgccat
      841 tgagccgacg tgcgcatcga ttaacgtgac gatgtcgatg tcaaccgccg cagctgatcc
      901 gctcgtcact ccccaagttg taaaagagca cggccttacg gcagaagaat tcgagcgcat
      961 aaagaaaatc ctcgggcgcg agccgaactt caccgagctc gggatcttct cggtaatgtg
     1021 gagcgagcac tgctcctaca agaactcccg caacgagctg aaaaagcttc cgaccactgg
     1081 gccgaatatt ctggtcaagg ccggcgaaga gaacgccggc gtggtcgata tcggcgacgg
     1141 ctgggccatc gcgttcaaaa tcgagagcca caatcacccc agcgcgattg agcccttcca
     1201 aggcgcggcc accggggtcg gtggcatcat tcgcgacatt ttcacgatgg gcgcgcgccc
     1261 ggagttttgc ctgaactcgc tccgcttcgg gccaattgta gcgcaagcct ctggcttgca
     1321 ggatcacaag cgggacgctc gcgttactct ggcgaatcgc cgcttgttta ctggcgtcgt
     1381 gagcggcatt gcgcattatg gcaactgcgt cggcatcccg acgatcggcg gtgaaattta
     1441 tttcgacgag agcttcgaag gcaatccact cgtcaacgtc ttttgcctcg gcgttctgcg
     1501 tcacgatcag ctcgcgcgcg gcactgcgcg cggcattggc aaccccgttt tttacgtggg
     1561 tgccgaaacg ggtcgcgacg gtttggccgg tgcagcgttc gcctcgcgcg agctgacgga
     1621 acaatcgcgc gaagatcggc ccgctgtgca agtcggcgat ccttttaagg aaaaactctt
     1681 gctcgaagcg tgtctggagc ttctcgcgca cgacgcagtc gcggggatcc aggacatggg
     1741 cgcagccggc ctgacctgtt cgacctgcga aaccgccagc cgcgccggca gcggaatcga
     1801 gattgacctc agcaaagtgc cgaaacgcga gccgggcatg acgccgtacg aaattctgct
     1861 cagcgaatcg caggagcgga tgctcatcat tgccaagcgg ggcaaagaaa atctcgtgcg
     1921 cgaaatcttc gagaaatggg acgtggcgtg cgccgagatc ggccgggtca cggacgacgg
     1981 cataatgcgc gtgcgcaaca acggcagcgt ggccgcggaa attcccgcga aaccgttggc
     2041 cgaggaagcg ccactctatt cgcgcgaagc aaaagaacct gtagccacgc gcttgtggcg
     2101 cgtcgagggc ggacggcccg cagagccgtg gctacaaaat ctccagaaga tcgacaatcg
     2161 cgaagcgttg cgccaactcc tacgcgatcc aactatcgcc tcgaaaaact gggtctatcg
     2221 gcagtacgac cataccgttc gcgccggcac gattgtcaaa cccggctccg atgccgccgt
     2281 gtttcatgtt aagtatgcga acaaaattct cgccgccacg accgactgta attcgatcta
     2341 ttgcgcgctc gatccgcgtg aaggcgcgaa gatcgccgtc gcggaagccg cgcgcaatct
     2401 cacctgcgcg ggtgcgaaac cgctagcagt gaccgataac ctgaacttcg gcaaccccta
     2461 caagccggaa aacttctggc aattgcgcga agccattgaa ggtatcgccg aggcctgccg
     2521 cgcattcggc acgccggtga ctggcgggaa cgtatctctg tataatgaga gccccgccgg
     2581 cgtcgtcgat ccaacgccaa ccattgccat ggtcggcctc attgacaggc cagagcacgt
     2641 cacgacacag tggttcaaaa agcaaggcga cgcgatcatt ctcgtcggca gtggcatggg
     2701 cttccagccc atgtcgcatg ggcaagatgc cgatgccgcg acgcatctac tcggtggctc
     2761 gcgatatctc aaagtctgtc acggcaagaa aatcggaccg ccaccacatg tcgatcttac
     2821 acaggagata aacgtccaaa gtgccgtgcg cgagttgatt cgcggcgggt tggtcaaaag
     2881 cgcgcacgat tgcagcgagg gcgggcttgc cgtggcgctg gcagaatgct gttttaatcc
     2941 cgagaaactt ttgggcgcga aagttgatct cggcgattgt agccacggcc ctgcgggccg
     3001 tcgggaagag acgggccaca ggcccgtggc tacagcactg ttcaacgaat cgcagtcgcg
     3061 catcatcatc tcggttgcgc ccgagagcat tgagcgcacg atcgcgatgt tgcgcgagcg
     3121 cggtgttcct cacgagcaat ttggcaccgt cggcggcgag gaattgcgta tcaccgtaaa
     3181 tggtgactcg ctcgcctggc cgattgtcga tctttacgac gactggtgga acgccattcg
     3241 tcgcacggtg gaaagcgact cggccgccga gcgcattccc agcttgtgat tggctggtct
     3301 atcgtgaatg tttccggtgt gaggctcccc ccgatttcca cgcaccaaga attgcccgac
     3361 cgcgctgcgc tgccgcagca cgagtgcggg ctgttcggcg tctttggcca cccgaaagcg
     3421 gcggtcctga cctattatgg gcttttcgcg ttgcagcatc gtggccagga aagcgctggg
     3481 attgtgacca gcagcggccc gggcacatcc tttctcattc acaaaggcat gggcctggtg
     3541 tcgcaagtgt ttggccaggc cgagttggag aaactgaaag gcacccgcgc gattggacat
     3601 gcccgctact cgaccaccgg gacgagcacg atcaaaaacg cgcaaccgtt cgtcgtagac
     3661 tgcgtccgcg gccaaatggc catcgcgcat aacggcaacc tgaccaatgc ggacatcctt
     3721 cgcgacgagc tcgagcggcg cggttccatc ttccaaacga cggccgatag cgaaatcatc
     3781 ctgcatctgc ttgcgcaacc tgccggcaac ggcagcaacg tcctcgcggc gttgcgcgcg
     3841 attaaaggcg cattctcgct tctaatcatg agcgagcgcg agctgatcgc ggtccgcgat
     3901 ccgttcggct ggcgaccgct ttcgttaggc aaaatcgacg gtgcttacgt tctcgcgtcc
     3961 gagacgtgcg cgttcgatct catccacgcc gatttcgtcc gtgaaatccg accgggcgaa
     4021 gtgctcatca tcgacgagaa cggtgtgcgc tcggaatttc cattcccgcc cgagcagccg
     4081 gctttctgca tgttcgaata cgtttatttc gcgcgaccgg acagcatgat cggcggtgtc
     4141 aacgtcggca aagtgcgcac tgagatgggc cgcgagctgg cgcgccgtta tcccgccgac
     4201 gcggacatcg tcattccggt gccggactcg ggaaactacg ccgcgctcgg tttcgccgag
     4261 gaattgaaga tcccgtttgc gcacgcgttt gtgcgcaatc actacatcgg ccgcacgttt
     4321 ttgcagccga gccagctcat tcgcgatttc gacgtgcgcg tgaagctgaa cctgatcaag
     4381 gaaatggtcg ccggaaaacg cgttgtggtt gtcgatgact cgatcgtgcg tggcacgacc
     4441 gcgcgcgcgc gcgtggtgaa cttgcgcgag gcgggcgcga aggaagtgca catgcgcgtg
     4501 agttgtccgc cgcaccggtt cgcctgccac tacggcatcg attttcccga ccccgagaaa
     4561 ttgatcgcga acaagatgtc gatggaagac atctgcaaat atctcggcgt cgacagtctc
     4621 ggctacttgg atgtcgaagg catggtgcgc gccaccggcc gcaaagtgaa tgagttttgt
     4681 ctcgcctgct tcaacggcca ttatcccctg cccgtcgatc cagcgctcga caagttcatc
     4741 atggaaaaac gcgaagcccg ctccaaagca ctcgctgacc aggaagcgca ccccacgttg
     4801 ttcgcggatc tgaagtagcc ggcgcgacgg cgttttctcc tgtcattccg agcgaagtcg
     4861 aggaatctct aattctttaa cagcaagaga tgtctcgact ccgctcgaca tgacaagaaa
     4921 acaaaaggct tacgcgcacg ccggcgtaga tgtcgatctt gccaaccggc tcaagcgccg
     4981 catccagcga ttcgttaggc gaacccacgg tccgcaggtg ctcggcaaaa ttggcggctt
     5041 cggcgggctc ttccgcgccg aatttcgcga aatgcgcgaa ccggtcctgg tctcgagcat
     5101 cgatggcgtc ggcacgaaga tgaagatcgc cttcgcgtta gatcgacatg acaccgtcgc
     5161 catggatctg gtgaaccatt gcgtcaacga catcgctgtc ctcggcgcga gaccactttt
     5221 cttcctagat tacatcggcg gcgaaaaact cgagccgcgt gtcttcgaac agttgctccg
     5281 tggctttgcg cgtgcctgtc acgccgctgg atgcgcgttg atcggcggcg agaccgcgca
     5341 aatgccggac atgtatcgca ggggcgaata cgatcttgtc ggctgcatcg tcggcgtggt
     5401 tgatcacgcg aacatcatcg atggccgaaa catcaaaccg ggcgacgtgg tcctcggtct
     5461 cgcctcgagc ggtctgcaca cgaacggtta ctcgctcgcc cgaaaaattt tgttcgagaa
     5521 tatgcgtctc aaaccttcat cgaagatcga cgacctgcgc ggcacagttg gcgatgagct
     5581 tttgcgagtg cataaaaatt atcagccgct gcttgcgaaa gcgccgagcg gtttaatcaa
     5641 aggtctcgca cacatcaccg gtggcggttt gattgacaat ttgccgcgga ttttgccgga
     5701 cgactgcgac gcaattattg agacgaaatc gtggcgagtg cccctgatct tccaaatcct
     5761 gcaagagaat ggaaacgtcg accgcaccga gatgtaccag gtgttcaaca tgggaatagg
     5821 aatggtcgcg gttgtcgcgg agcgcgacgc aaatcatatg atgtcgattc tccgcgcgag
     5881 acggatcggc acgatcgagc gcggaaacag aaaaacgcaa ttattgtagc ggcagtctgt
     5941 gactgtcgct aattttcgtc cctcgaagaa aaaagtgcga tggtcagaga ccgccgctac
     6001 aagacgtcat ggagaagcgg cgcctcggca aaacggagat ggacgtgacc gtgctcggct
     6061 tcggcgggtc tgagatcggt tacgagcgcg tggcgcaaga tgcggtcgag cgtttgttga
     6121 acagcgcgct tgacgccggg ttgaacgtca tcgacacggc cgaatgttac tacggcagcg
     6181 aagagttgat cggacgcgcc gtcggcaaac ggcgcaacga attttatctc tttaccaaat
     6241 gcggtcaccc gcacgggatg gaaagcgccg ccaactggtc aaaggtttca attctgcaaa
     6301 gcattgagcg cagtttacgc cggctgcaaa ccgaagttct cgatcttgtg cagttacaca
     6361 gttgttccga gtcggaattg cggaacggcg aagcgattga tgcatcgcaa accgcgcgtg
     6421 aacgcggctt gactcgttac atcggctaca gcggcgacag tcgcgccgca cattatgccg
     6481 tggaatgcgg cgcattcgac acgttacaaa cttccatcaa tatcgccgac caggaggtga
     6541 ttgagttgac gctgccaatc gctcgacaaa agcagatggg cgtgatcgcg aaacgcccga
     6601 tcgccaacgc gacatggaag accggccatc gcccgccgga ttcttatcat cacgaatatt
     6661 gggagcggct ccggcgattg aattacgatt tcctgcgcac tcggccgctc gaagagagca
     6721 tctcaatcgc tctgcgcttc acgctcagcg tccccggcgt gcacaccgcg attgtcggca
     6781 ccacgaatcc cgagcgctgg cagcaaaacg cgaagaagct tgaggctggt gcgctgcggg
     6841 acgcagaatt caacgcgatc cgccaccgct gggaagaata cgccccgcga gcttggatcg
     6901 ggcaaatata actacttgtc atttcgagcg gagcgcagcg cagtcgagaa atctcttgct
     6961 gttgaaaatg agagatgtct cgacttcgct cgacatgaca aacattcgcc accgctggga
     7021 agaatacgcc ccgcgaactt ggatcgggca aatatgaaca agtcttaagt gctaagt
//
LOCUS       DASTDG010000051         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_313116, whole genome shotgun sequence.
ACCESSION   DASTDG010000051 DASTDG010000000
VERSION     DASTDG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313116"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFO30_01625"
     CDS             complement(<1..956)
                     /locus_tag="VFO30_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HET9418015.1"
                     /translation="MKAPGKFKLAVYLFGFGGAALFTVLLIRQGASAVALAVAAAGWA
                     IAAIAVYHLFVPLCLDAVAWWVLFPSFCRPPLRKLFWMRWVGESVSTLVPSAAVGGDI
                     VRARLATINGAPLALSAATVLVDVTLGVFTQAAFTLLGLALLVDVTGQKSFVGPTLLG
                     TLAGVGAIAGFYFVQRMGMFRFLATIVAKLASSDEWNSLIQGGTTLDQTIRDLYARKR
                     GVIACCVVTIVSLIGGSGEIWIGLHALGLQATVVNAVILQSMALTIRSVAFPVPGGVG
                     VQEGGYVAVGNFLGIPGEAAFALSLIARVRELTIGVPGLLIW"
     gene            complement(1017..1742)
                     /locus_tag="VFO30_01630"
     CDS             complement(1017..1742)
                     /locus_tag="VFO30_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9418016.1"
                     /translation="MTKHEMCLILTANFVTNAFLPLQNCVALSVANPLAGVDHGLANW
                     FHAHLTRTFVSVLRGFTEFGASEWICVVLSALVLFFIWKRWWPSLVTLVVAIPGGMLL
                     NELLKLLVHRHRPFVDGWFVDWSGYSFASGHTIGATLLYGQLALFVLPSIKSRRWRAL
                     IIATAAVFVALVGFSRIALGAHYFTDVVGAIVLGISWLMLCLIATKPVRRRAALPAIL
                     PQAPEPVLVPVEATNSTAPTSLR"
     gene            1877..2788
                     /locus_tag="VFO30_01635"
     CDS             1877..2788
                     /locus_tag="VFO30_01635"
                     /inference="COORDINATES: protein motif:HMM:NF039394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HET9418017.1"
                     /translation="MKIIRLQFFTILLFAIGSVLAGTEPVVETAPAPTPADQNSRDIF
                     NYETTYTFRSDFYDDHGKLGHGDSLYDQFSYAHRFLITGNWYFRAGVEYERFDFDGTD
                     NGLPDHLQTVHALLALEYVIHDHAGVGLEIDPGPYFQSSITGDSIDVPWKAFVTFPLK
                     KDRIFGVIGVGGALYQNPVVAPGGGLIWLFTDKFRLEGVFPKPALVYNPNDDWEFRLA
                     GNLFFESYRTEDVITPEKKLRVHNAVVQYSEERAGFQATYSHFKPFDITLDAGYTFKR
                     DFDFFRKEASAKLDPAPYVRLAIEAKF"
     gene            complement(2785..3192)
                     /locus_tag="VFO30_01640"
     CDS             complement(2785..3192)
                     /locus_tag="VFO30_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9418018.1"
                     /translation="MMRTTGFCVVICAVSALLAPEIRAAPSEPQNTTPQQVFDGMRQS
                     FRADKAKGVHAKYQWQLSGPNGGEWWIDVNDGAFKMGRGKIDNPTVSFITSDNDWVAM
                     SNGKLKGTWAFLTGRLKVRGPQSVARKLDEIFP"
     gene            3321..3536
                     /locus_tag="VFO30_01645"
     CDS             3321..3536
                     /locus_tag="VFO30_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418019.1"
                     /translation="MVPRPRVFPDHPHGIAGINHFRKDDEISARAFRARRQVAELSNI
                     SIEIAQRARDLGGGDFHMGGASVSLAV"
BASE COUNT          810 a         1101 c          978 g          679 t
ORIGIN      
        1 tgccagatca gcagacccgg aactccgatg gtcagttcgc gcactctggc gatgagcgac
       61 agcgcaaagg cggcctcacc gggaatcccg aggaaattac cgaccgccac gtagccgcct
      121 tcctgcacgc cgactccccc gggcaccggg aaagcgaccg agcggatggt cagcgccata
      181 ctctgcaaaa tgaccgcgtt aaccacggtt gcctgcaatc caagggcgtg caggccgatc
      241 caaatttcac ccgagccgcc aatcagcgac acgatcgtta ccacgcaaca cgcaatcaca
      301 ccgcgttttc gcgcataaag atcgcgaatc gtttggtcga gtgtcgtgcc gccctggatc
      361 aaggagttcc attcgtcgga gctggccagc ttcgcgacaa tcgtggcgag gaagcggaac
      421 atccccattc gctgcacaaa atagaaaccg gcaatggcac cgacacccgc cagggtgccc
      481 agcagggtcg ggccgacaaa acttttttgt ccggtaacat cgacaagcaa cgccagcccg
      541 agcagggtga aagccgcttg cgtgaagacc ccgagcgtaa catcgacaag caccgtcgcg
      601 gcagacagcg ctagcggcgc gccgttgatc gtcgccaagc gcgcgcggac gatgtcaccc
      661 cccaccgcgg ccgacggcac aagcgtgctg accgattcgc cgacccagcg catccagaac
      721 agcttgcgca aaggaggacg gcagaatgac ggaaagagca cccaccacgc gaccgcatcg
      781 aggcagaggg gtacgaaaag gtggtagacg gcgatcgctg cgattgccca gccagcggct
      841 gcgaccgcca acgccaccgc tgaagcgccc tgccggatga gcagcacggt gaagagcgct
      901 gccccgccaa atccaaaaag ataaacggcg agtttgaact tgccgggcgc cttcattgtt
      961 cctgaatcgt ggccaagcgc acgagcgcgc tcatcacgag ttcgctgctg ctgacgtcag
     1021 cgcaacgacg tcggcgccgt cgagttggtt gcctcgaccg gaacgagaac cggctcgggc
     1081 gcctgaggca aaatagcggg aagagccgca cgacggcgga cgggctttgt cgcaatcaga
     1141 caaagcatca accatgagat gcccaggaca attgcgccca ccacgtcggt gaagtaatgc
     1201 gccccaagcg caatccggct gaaaccaacc aaagcaacga aaaccgcggc tgtggcaatt
     1261 atcagcgcac gccagcgtcg gctcttaatt gagggcagaa caaacaacgc gagctgccca
     1321 tagagcaacg tggcgccgat cgtgtgcccg ctagcgaaac tatatcccga ccagtccaca
     1381 aaccagccgt caacaaatgg ccggtgacga tggacaagca gtttgagcag ttcgttgagc
     1441 agcattccac cgggaatggc cacaaccagg gtaaccagcg agggccacca gcgcttccaa
     1501 atgaagaaca gcacgagcgc ggaaagcacg acgcaaatcc actcactcgc accgaactcg
     1561 gtgaagcccc gcagcacgct tacgaatgtg cgggtcaggt gggcatgaaa ccaattggcg
     1621 agcccgtggt cgacgccggc caatggattt gctacgctca gcgcgacgca attctggagg
     1681 gggaggaacg cgttggtcac aaaattggcc gtcaagatta agcacatttc atgcttcgtc
     1741 aagtcatgaa cgctcgtcga tgttaaaaaa gatgtcccag gtgcgcagcc tgatcaggct
     1801 tgcgcaaagt tgttttctcc gatacatgcg tggctgccag ctcgcccgat tactcgatgt
     1861 tgactttgcc tagcctatga aaattatccg gctccagttt tttacaatcc tcctttttgc
     1921 aatcggctcc gtcctcgcgg ggacggagcc ggtggtcgag actgcacccg caccgacgcc
     1981 ggcagaccaa aactcgcgcg acattttcaa ttacgagacg acttacacct ttcgcagcga
     2041 tttctacgat gaccacggca aacttggcca cggcgattca ctctacgacc agttcagcta
     2101 tgctcatcgc tttctgatta ccggaaactg gtacttccga gccggggtcg aatacgagcg
     2161 attcgatttc gacggcacgg acaatggtct gccggatcat ctccagacgg tccacgcttt
     2221 gctcgccctc gaatacgtca ttcacgatca tgccggcgtg ggcctcgaga tcgaccctgg
     2281 accttacttt caaagcagta tcacgggcga ttcaatcgac gtgccgtgga aagcgttcgt
     2341 cacgtttccc cttaagaaag acagaatttt cggagtcatc ggcgtgggcg gcgcgcttta
     2401 tcagaatccg gtcgtcgctc cgggcggcgg attgatctgg ttgttcaccg acaagttccg
     2461 ccttgaaggc gtgtttccaa aaccggcgtt ggtttacaat ccgaacgacg attgggagtt
     2521 tcgccttgcc ggcaatttgt ttttcgaaag ctatcgcacc gaggacgtca tcacgccgga
     2581 aaagaagttg cgcgttcata atgccgtcgt gcaatacagc gaggaacgcg ctggcttcca
     2641 ggccacctat tcgcacttca agccttttga tatcaccctc gacgccggct acacgttcaa
     2701 gcgggacttc gatttcttcc gcaaggaagc cagcgcgaaa ctcgatcctg ccccctacgt
     2761 caggcttgcc atcgaagcga agttctaggg aaaaatctca tctagctttc gcgcgaccga
     2821 ttgcggcccg cgaactttca atcggccggt caaaaaggcc cacgtgcctt tcaatttccc
     2881 gttcgacatc gcgacccaat cgttgtcgct ggtgatgaac gatacggtcg gattatcgat
     2941 cttgccccgg cccatcttga atgcgccgtc gttcacgtcg atccaccatt cgccgccgtt
     3001 cggcccgctt agctgccact gatacttcgc gtgcacgcct tttgccttgt cggcgcgaaa
     3061 gctttggcgc atgccgtcga acacctgttg cggcgtcgtg ttctgcggct cgcttggagc
     3121 tgctcgtatt tcaggggcca acaacgcact tacagcacaa atcaccacgc aaaacccggt
     3181 cgttctcatc attttcggtt cggtcacgcc aagtgaactg gccgtgtcag ttcgtctgat
     3241 acaaatcgcc gcgccgatga tcgaacaccg gaatccgttg acgcacgtcc gtgattacct
     3301 gctcggaaat ttcggcggcc ttggttccac ggccgcgcgt gtttccggat catccccatg
     3361 gaatagccgg tatcaaccat ttccggaaag acgacgagat cagcgcccga gcctttcgcg
     3421 cgcgccgcca agtcgcggag ctttcgaaca tttccatcga gatcgcccag cgcgcacgag
     3481 atctgggcgg cggcgatttt catatgggtg gggcaagcgt ctcgcttgcc gtttaaaagc
     3541 gcaagccaga ggctcgcacc actttagc
//
LOCUS       DASTDG010000052         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_787934, whole genome shotgun sequence.
ACCESSION   DASTDG010000052 DASTDG010000000
VERSION     DASTDG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787934"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFO30_01650"
     CDS             <1..322
                     /locus_tag="VFO30_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418020.1"
                     /translation="YIAKPHSDAILAQDLIPLPALTAMTCPPNDRVTTFYDESERLVR
                     FLASLDKPRFLALLDALGRHQPFESAFSRIYIGQFQNTAALEEKFREYAAKDFGTTLQ
                     QVSD"
     gene            354..1655
                     /locus_tag="VFO30_01655"
     CDS             354..1655
                     /locus_tag="VFO30_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418021.1"
                     /translation="MRRRSRTNYPSPSEKPIHLVDVRGACEKLVVPKTQTKSATANIH
                     AVVGSDEAEVKRVAAELAQNLTPAAAGDFGLEIIDGAVDNAEQAVARIRSTVEALQTL
                     PFMGDGKVVWLKNANFLGDTPTGRSAIVLSALQELSELISGGIGADVTFLLSAAEVDK
                     RRTFYKSLAKSTELQVFDRLDSTRTGWEEEAIEAVRTRAKKRKLQFDDDALDLFVLLT
                     GGDTRQIDNELEKIDIFLADRGTSFQPLGQAGISPAITAAKMAAGPTAGTAVPRVTVD
                     VVRELVPLTRGGVVFELGNALAEHDLERGLRLVRRLLDQGETALGILLVAILPTVRNL
                     LLAKDLMERHRLPRPPVPFQFISAINRLPAEATAHLPRKKDGSINAYALGIAAQNAHR
                     FKTEQLVDGLNACLDANLKLVTTQLEHELVLTELVVKLLTE"
     gene            complement(1712..2140)
                     /locus_tag="VFO30_01660"
     CDS             complement(1712..2140)
                     /locus_tag="VFO30_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418022.1"
                     /translation="MPDPLEPTQPPPPMPPEPTTRSTTTGLPSNIAAALACIPLVGGI
                     IFYILEKRDGFVRFYAMQSIIFGAAWILFDIVSKILFAIFGSIPAIGGLLVFFWAIVQ
                     ALVHIAFLVIWIIAMVKAFTNVRWEIPYVGPVARKQVGEA"
     gene            2265..>2495
                     /locus_tag="VFO30_01665"
     CDS             2265..>2495
                     /locus_tag="VFO30_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9418023.1"
                     /translation="MSRRPQSAYTLIELLVVILVVFILASIAYPTYTSAIARARATQD
                     MNNLRQIGLAMQTYLNDNDQRLPGSTTWPGTSA"
BASE COUNT          557 a          718 c          716 g          504 t
ORIGIN      
        1 ttacatcgca aaacctcact cggatgcgat cttggcccaa gacctaattc cgttgccggc
       61 gctgaccgcc atgacttgtc caccaaacga tcgcgtgacg acgttctatg atgaatcaga
      121 acgactcgtc cggtttctcg cgtcgctcga caaaccgcgg tttcttgctc tgctcgatgc
      181 attggggcgg caccaaccat tcgagagcgc gttctcgcgc atctatatcg ggcaatttca
      241 aaacaccgct gcactcgaag agaaatttcg cgaatacgcg gcaaaagatt tcggaaccac
      301 tctgcaacaa gtaagcgact aaaggtggaa cgcgctctcc gagcgcgttg ctattgaggc
      361 ggcgaagccg cactaattac ccatcgcctt cggagaagcc gatccacctt gtcgatgttc
      421 gcggcgcatg cgaaaaactg gtcgtgccga agacccagac gaaatctgcg acggcaaaca
      481 ttcacgcggt cgttggctcc gatgaagcgg aggtcaagcg agtcgcggct gaattagcgc
      541 aaaaccttac gcccgccgcg gcgggcgatt tcggcttgga aataatcgat ggagcggtcg
      601 ataacgcgga gcaagccgtc gcccgcatca gatcgacagt cgaagcgttg caaacgctgc
      661 cattcatggg cgatggaaaa gtcgtgtggt tgaagaacgc gaattttctc ggcgatacgc
      721 cgaccggccg ctctgcgatc gtgctcagcg cattgcaaga gttaagcgaa ctgatcagcg
      781 gcggcatcgg agcggacgtg acgtttttgc tcagcgccgc ggaagtggac aagcggcgga
      841 cgttttacaa atcgctggct aaatccaccg agctgcaggt ttttgaccgg ctcgattcca
      901 ctcgcactgg ctgggaggaa gaagcaatcg aggcagtaag gacccgcgca aagaaacgaa
      961 aactgcaatt cgacgatgat gcgctcgatc tcttcgtcct gctcaccggc ggcgatacgc
     1021 ggcaaatcga taacgagctg gagaagatcg acattttttt ggctgatcgt ggcacaagct
     1081 tccagccttt ggggcaagcg ggcatctcgc ctgccatcac ggcagccaag atggctgccg
     1141 gccccacagc cgggacggcc gtgccacgag tgactgtcga tgttgtccgt gagctggtcc
     1201 cgctcacgcg tggtggcgtg gtgtttgaat tgggaaacgc cctcgccgag catgacctcg
     1261 aacgaggcct gcgactcgtt aggcgcttgc tcgatcaggg cgaaactgcg ctcggcattt
     1321 tgctcgtcgc gattttgccg acggtgcgca atttacttct cgctaaagac ttgatggagc
     1381 gccatcgctt gcctcggccg cctgtgccgt ttcagttcat ttcggcgatt aatcgtttac
     1441 cggcggaagc gaccgcgcac ctgccgcgca aaaaagacgg ctcgatcaac gcctatgctc
     1501 ttggcattgc tgcacagaac gcgcatcgtt tcaaaaccga gcagttggtc gacgggctga
     1561 acgcgtgtct cgacgcaaac ctgaagctcg ttaccacgca gctcgaacac gaattggtgt
     1621 taaccgaact ggtcgtgaag ctgctgacgg agtaacgcaa gcctctggct tgcgaaattt
     1681 gttggaaggc aagcgagacg ctctccctac cttaagcttc gccgacctgt ttgcgcgcaa
     1741 ccggtccgac gtagggaatt tcccagcgta cgtttgtgaa cgctttcacc atcgcgatga
     1801 tccagatgac gaggaaggcg atgtgaacca gcgcctgaac aatggcccag aaaaacacca
     1861 gcagaccccc gattgccgga atggagccga agatcgcgaa cagaattttt gagacgatat
     1921 cgaacagaat ccaggcggcg ccaaagatga tcgattgcat ggcgtaaaag cggacaaacc
     1981 cgtcgcgctt ttccaggatg taaaaaatga ttccgccgac gagcggaatg caggcgaggg
     2041 cggcggcgat attggatggc agaccggtcg ttgtcgatct tgtggtgggc tccggtggca
     2101 ttggcggcgg cggctgggtt ggctccagcg gatcgggcat gaggtagtta cactaggggc
     2161 tgtcggcttt gtcaaccccc gaatgcgctc cgccggcgcc gcggacaacc gctgaatgcc
     2221 ggtttttcgt ttcgctcgca accgaaatgc aatacgctgg atcaatgtcc cgtcgtccgc
     2281 agagcgccta tacgctgatc gagctgctcg ttgtcatcct tgtcgtcttc attctggcct
     2341 caattgctta ccccacctac acgagcgcga tcgcccgcgc ccgcgccacg caggacatga
     2401 acaatctgcg ccaaatcggc ctcgcgatgc agacctatct caatgataac gaccaacgcc
     2461 tcccgggatc aaccacatgg cctggcacaa gcgct
//
LOCUS       DASTDG010000053         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_899837, whole genome shotgun sequence.
ACCESSION   DASTDG010000053 DASTDG010000000
VERSION     DASTDG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899837"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..354
                     /locus_tag="VFO30_01670"
     tRNA            280..354
                     /locus_tag="VFO30_01670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:312..314,aa:Val,seq:gac)
     gene            complement(444..1442)
                     /locus_tag="VFO30_01675"
     CDS             complement(444..1442)
                     /locus_tag="VFO30_01675"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9418024.1"
                     /translation="MSFIRLLVDLAQCRSWGIARVTNFCRFTRFSKIFLPEERHRLAP
                     RNSRLFIRRMFGVTTSDDYRPVAWMGRYPLDVTTMLVALHVVCAIIAALFGGYGLFGY
                     AMFDSAYIWSGQLWRFITYAFIHPAGSSALLWFAVEMYMLFIFGREVERFFGRRAYLA
                     LYGLLLLLPPIVLTLWGLTQRTGMTGSATLHFGVFIAFVILYPNVQFFAFISAKWVAV
                     ILAAIGTLSALAARDWQYLVILWTSIGAAFLFVQLHGAGPELVWWERVKGMIRPRPKL
                     HVVPKPTTRPPAERDDVYASIDPILDKISKSGIGSLTAQERRQLDRARNRLLNEPK"
     gene            1432..3237
                     /locus_tag="VFO30_01680"
     CDS             1432..3237
                     /locus_tag="VFO30_01680"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET9418025.1"
                     /translation="MKDIAPAISPPAGIGNNKPANQAVLDWVHEVELLAKPQNIFWCD
                     GSDREQEYLLGEAMHEGVLIKLNQQKAPRSYLHRSNPNDVARVEQFTYICTPTQEEAG
                     PTNNWSEPAETYSKLHGLLKGAMRGRTMFVIPYIMGPANSPLTKVGFEITDSIYVVLS
                     MRIMTRMGEVAVKRLGNDPAAEWNRGVHSLLDVDPARRFICHFPQDNTIISVGSGYGG
                     NVLLSKKCLALRIGSYLARKQGWMAEHMLILGAESPEGRKHYIAAAFPSACGKTNFAM
                     LIPPKHFAGWKITTVGDDIAWMQVRDGRLWAVNPENGYFGVVPGTNYKSNPNAMKSIE
                     HDSLYTNVALTDDGDVWWEGKDGPPPTHAIDWRGRDWTPASKEKAAHPNSRFTTPVRN
                     NPVLDPDFEKGEGVPISAVIFGGRRSTTVPLVYQAFDWDHGVYLGATMASETTAAAAG
                     AVGQVRRDPMAMLPFCGYNIGDYFRHWIDIGTRLTNPPLIFNANWFRKGMAGNFLWPG
                     FGDNMRVLKWIIDRCQGSGGALKSPIGWIPGLHDLDLAELDIDHKCVAALLGIDHEEW
                     QKELAAHKRFFDSLGGVVPEELLDQRKQLAARFKL"
     gene            3345..>3759
                     /locus_tag="VFO30_01685"
     CDS             3345..>3759
                     /locus_tag="VFO30_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418026.1"
                     /translation="MGSKLAKKECVTAPFERGCVEIRGARQNNLKGVDLDLPLGKLTV
                     VTGPSGSGKSSLAFETIYAEGQRRYVETFSPYMRQFLDRMDKPRVDDIRGIPPAIAIE
                     QSNPVKTSRSTVGTMTEINDYLKLLWPRLAKAFCPS"
BASE COUNT          864 a         1132 c         1071 g          692 t
ORIGIN      
        1 gtatccggac agcgaaatgc tcgaccgcat cctggcgaaa ggcccgatca ccgttttgaa
       61 gaaaccgctc aaggccgagc aactccaaca gaccgtccgc atcctcggcc acaaagaagc
      121 ggtcaagctc gccgcgtgaa actcgctgcg cgagttttgt gatccgcgat cggtgaatag
      181 tgatcggtag ttcagcagtt cacccgctgg atcgacgatc acactgctcg atcttgacgg
      241 atcagcgatc gccgatcact tatcacttcg gtcgaaaacg cgcggttagc tcagtggtag
      301 agcactacct tgacacggta ggggtcactg gttcgaaccc agtatcgcgc accatcctca
      361 tgcgcagttg gatctgaact tccgcacgtc atctgccagg cgctcgagcc acgtgcccgc
      421 tggcaacttg aactagagcg cgtttacttc ggctcgttca ataaccgatt acgtgcgcga
      481 tcgagctgcc gccgttcctg cgcagtcaaa ctgccaatcc ctgacttgga aattttgtcg
      541 aggatcggat caatcgaagc gtaaacgtcg tcgcgttccg ccggcggacg cgtagtcggt
      601 ttcggcacga cgtgcagctt cggtcgtggg cggatcattc ctttcactcg ctcccaccag
      661 acaagctccg gtccggcgcc gtgcaattga acgaaaagga atgccgcacc gatactcgtc
      721 cacaaaataa cgaggtattg ccaatcgcgg gctgcgagtg cgcttaaggt gccgatcgcg
      781 gccaaaatga ccgcgaccca tttcgcggag atgaacgcaa aaaattgcac gttcggatag
      841 agaatgacga aggcgataaa aactccgaag tgcaatgtgg ctgagccggt catgccagtg
      901 cgctgagtta gtccccacaa cgtcagcacg attggcggca acaacagcaa taggccgtac
      961 agcgcgagat aggcgcgccg cccgaaaaag cgttcaactt cgcggccgaa aataaacagc
     1021 atgtacattt cgacggcgaa ccacagcaac gcactactgc ccgccggatg aatgaaagca
     1081 taagtgataa agcgccaaag ttggcccgac caaatgtagg cgctgtcgaa catggcgtaa
     1141 ccgaacagcc cgtagccgcc gaaaagggcg gcgatgatgg cacagaccac gtgcagcgcg
     1201 accaacatcg tggtcacgtc gagcgggtat cgccccatcc acgctacggg ccgataatca
     1261 tcggaggtcg tcactccgaa catgcgccga ataaacagcc gcgagtttcg cggcgcaagc
     1321 cggtgccgct cttctggcaa aaaaatcttg gagaagcggg tgaaccggca gaaattcgtc
     1381 actcgcgcaa tgccccagga tcgacattgc gcaagatcga caagcaaccg gatgaaggac
     1441 attgcgccag ccatttcgcc gcccgccggc atcggcaaca acaaaccggc gaaccaggcg
     1501 gtgctcgact gggttcacga agtcgagctg ctcgcgaagc cgcaaaatat tttctggtgc
     1561 gacggctcgg atcgcgaaca ggaatacctg ctgggcgaag cgatgcacga gggagtgtta
     1621 atcaaactca accagcaaaa ggccccgcgc tcatatctgc atcgctcgaa cccgaacgac
     1681 gtcgcccgcg tagaacagtt cacttacatc tgcacgccga cgcaggaaga ggctggcccg
     1741 acgaacaact ggagcgaacc agccgagaca tattccaagc tgcacggtct actgaaaggc
     1801 gcgatgcgcg gccgtaccat gtttgtcatt ccctacatca tgggcccggc caattcgccg
     1861 cttaccaaag tggggttcga gattacggat tcgatttatg tcgtactcag catgcgaatc
     1921 atgacgcgca tgggcgaagt cgcggtgaaa cgcttgggca atgatcccgc tgcggagtgg
     1981 aaccgtggtg tgcactcatt gctcgatgtc gatcctgcgc ggcggttcat ctgccatttt
     2041 ccgcaggaca acaccatcat ctccgttggc tccggctacg gcgggaatgt gttgctcagc
     2101 aaaaaatgtc tggcgttgcg catcggttcg tatcttgcgc gcaaacaggg ctggatggcc
     2161 gagcacatgt tgatccttgg cgccgagtcg cctgagggcc gcaagcatta catcgccgcg
     2221 gcgttcccga gcgcgtgcgg caaaaccaat ttcgccatgc tcattccacc gaagcacttc
     2281 gccggctgga aaatcaccac cgtcggcgac gacatcgcct ggatgcaggt gcgcgacggt
     2341 cgactctggg cggtgaatcc ggagaacggt tacttcggcg tcgtgcccgg caccaattac
     2401 aaatcgaacc caaacgcgat gaaatcgatc gagcacgatt cgctttatac caacgtcgcg
     2461 ctcaccgacg acggcgacgt ttggtgggaa ggcaaagacg gcccgccgcc cacgcacgcg
     2521 atcgattggc gcggacgcga ttggacgccg gcatcgaagg aaaaagccgc gcacccgaac
     2581 agtcgattca cgacgccggt gcgcaacaat cccgtgctcg atcccgattt tgaaaaaggc
     2641 gaaggcgtgc cgatcagcgc ggtcattttc ggcggtcgcc gcagcacgac tgtgccgctc
     2701 gtctaccagg cgttcgattg ggaccacggt gtttatctcg gcgcaacgat ggcgtcagaa
     2761 acgactgcgg ccgcggctgg cgccgtcggt caggtgcggc gcgatccgat ggcgatgctg
     2821 ccgttctgcg gctacaacat cggcgattat ttccggcact ggatcgacat cggcacccgc
     2881 ctaacgaatc cgccgcttat tttcaacgcg aactggttcc gcaaaggcat ggctggcaat
     2941 tttctctggc ccgggttcgg cgacaacatg cgcgtgctca aatggatcat cgatcgctgc
     3001 caaggcagcg gcggcgcgct caaatccccg atcggctgga tacccggtct ccacgatctc
     3061 gatctcgccg agctcgatat cgatcacaaa tgtgtcgcgg cgctgctcgg aatcgatcac
     3121 gaggaatggc aaaaagaact ggctgcacac aaacgcttct tcgattcact cggcggcgtc
     3181 gtgcccgaag aactgctcga tcaacgcaaa caactcgccg cgcggtttaa gctgtagccg
     3241 tcgccttgtg ggcgacgtgg tgcgaacgtg gctcagcgga acgcttcgcc cagcgaaacg
     3301 gctgcagaat ttgcgcgctg cgtgcgcttc tgaaataatc tccagtgggt tcgaagctgg
     3361 caaaaaaaga atgcgtcacc gcgccgtttg agcggggctg cgttgaaatc cgcggggcgc
     3421 gccagaacaa tctcaaaggc gtcgatctcg accttccgct cggcaaactg actgtcgtca
     3481 caggcccaag cggctcgggg aagtcgtcgc tcgcattcga aaccatctac gccgaaggcc
     3541 agcggcgtta tgtcgaaacg ttttcccctt acatgcggca gttcctcgac cggatggaca
     3601 aaccccgcgt ggacgacatc cgcggaatcc caccggcgat tgcgatcgag caatcgaacc
     3661 cggtcaaaac atcgcgctca actgtcggca cgatgacgga gatcaacgac tacctgaagt
     3721 tgctctggcc acgcctcgcg aaagcctttt gcccaagct
//
LOCUS       DASTDG010000054         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_614529, whole genome shotgun sequence.
ACCESSION   DASTDG010000054 DASTDG010000000
VERSION     DASTDG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614529"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="VFO30_01690"
     CDS             complement(<1..1967)
                     /locus_tag="VFO30_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9418027.1"
                     /translation="MADQPSDSQHRRRRQGDGQTAWLSNSAIALLPVLACFVGGSTQK
                     WAEGIVVTLLGLVLLVRPPRVSLGHAINGVLLALIVLAAIAFLPSGWFFIPPWRTTLA
                     NEFKIALPSTVSPQPWITTTCLISLVAGVSWLYLVSTQDVDLHAVRWQLRLFVVGIVV
                     LAAFSVGFYMAHMSPAFWNNPRGFGPFPNRNQTADLFGISAIVLLACAQDDLRFGRKR
                     WVMWIPGLALLVAGLVLNLSRAGLVILVGGSALWIAIVALRQRSPGWIALGVSFLLLL
                     FSALLLGGGETLERFHLRGLQALGISSDFRWLIFKDTFRMIAASPWCGVGLGNFEAVF
                     ALFRAVSWADTRSLHPESDWLWVWSELGWPAVIVILVASALFIRRVFPLREGTNQRLR
                     LAALIGAILFGLHGIIDVSAHRVGTAFAGLFLLGLTLHRPLGLPSRRFVGVIFRVLGL
                     LLIVSGASWVIAARGQSLLPGSVGVTNAKQLAAAANSEYDFAEAIRMATRGLEWAPLD
                     WQLYFTRALAEVANKQPELALRDFRRASFLEPNGFEVPLAEGNAWLSTQSVYAANAFR
                     EALRRAGAREAEVFAIILTNASQQNPPVAHMMEEIGLARHELVLPYLNRVAGETFQRA
                     VNHVLRNDPELSTFSEPEKFALFTLWSERGDL"
     gene            2117..3526
                     /locus_tag="VFO30_01695"
     CDS             2117..3526
                     /locus_tag="VFO30_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET9418028.1"
                     /translation="MITQRTEGLYRLFLLCQIVIVAALFWLGVWIIVTFYAPGAGVEF
                     TWRRYSIYCGCIVLGIWIESLSRSGSRNYFLQNELLLQHRLSLRQTIAGIGVLVFYLI
                     GTKDAFISRVFFFNFVPWLYVALLFSHHYLPSLLARAFFTLRDEKTLLVGSTARAAHL
                     RTWLHRKAEIGLRTVGLVCDDDMENHDSSIPVLGKPSELEKIIPARGITQIILLEFPL
                     FTEANRNIIQICDRLGIRLLIVSDLEEKLRHPVTHFEDDGLRFIALREEPLENPLNRF
                     FKRAIDLAISIPVMFFIFPICAAAVWIAQRFQSRGPLFHVQTRAGMQNRQFKIWKFRT
                     MHVSNGDIARQAHHDDERVYPLGKWFRKLSIDEVPQFWNVLRGDMSIVGPRPHLIEHN
                     NQFSRLLENYHVRAFVKPGITGLAQVRGFRGEARNNSDIANRVACDIEYLENWNLSLE
                     CGIILRTFAQLIVPPRTAY"
     gene            complement(3523..>4025)
                     /locus_tag="VFO30_01700"
     CDS             complement(3523..>4025)
                     /locus_tag="VFO30_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9418029.1"
                     /translation="LRNISPDTNYVAIEPNRQTAERLRDSVGESVQVLNIAMSDRSGE
                     LGFTSGVTSGVFRVSAREAAEMKVHCERLDALSLPQTDLVVKIDVEGHELPVLRGAAR
                     LFEEQRVKVIYLDGYSDGAIPDFLRERDFALYHGRTLVPCGPEAPRDSLLAIHRSRLQ
                     ALEARD"
BASE COUNT          879 a         1288 c         1120 g          738 t
ORIGIN      
        1 aggtcacctc gttccgacca caatgtaaac agcgcgaact tctccggctc ggaaaacgtg
       61 ctcagctcgg gatcattccg caaaacgtgg ttgaccgcgc gttgaaacgt ctcgccggca
      121 acgcgattga gatatggcaa caccaattcg tgccgcgcca atccaatctc ctccatcatg
      181 tgcgccaccg gtggattttg ctgtgacgca ttcgtcagga tgatcgcgaa aacttccgcc
      241 tctcgcgcgc cggctcggcg cagcgcttcg cgaaacgcat tcgccgcata aacagactgc
      301 gtcgacaacc aggcattgcc ctccgccagc ggcacttcga atccgttcgg ctcgaggaaa
      361 ctcgcccgcc ggaaatcgcg tagcgcaagc tccggctgct tattcgcgac ttcggcaagt
      421 gcgcgcgtga agtaaagttg ccaatcaagc ggtgcccact cgaggccgcg cgtagccatt
      481 cggattgctt ccgcaaagtc gtactcgctg ttagccgcgg cggcgagttg cttggcgttg
      541 gttacgccga ccgagccggg tagcaaagat tgtccacgcg ccgcaatcac ccaggacgcg
      601 ccgctcacaa tcagtagaag cccaaggaca cgaaagataa cgccgacgaa ccgacgggac
      661 ggcaatccca gcggccggtg cagcgtcagc cctaacaaaa agagaccggc aaacgcagtg
      721 ccaaccctat gggcggacac atcgataatt ccatgtaaac caaacaaaat ggcgccgatg
      781 agcgcggcca gacgaagccg ttgatttgtg ccttcgcgga gcggaaacac gcgccgaata
      841 aacagggctg acgcgacgag aatgacaatt actgcgggcc agccgagctc gctccaaacc
      901 caaagccagt cgctctccgg gtgcagcgag cgcgtgtccg cccacgacac agcgcgaaaa
      961 agcgcaaata cggcttcgaa attgccgaga ccgactccgc accatggcga tgcggcgatc
     1021 atgcgaaatg tgtctttgaa gatgagccag cggaagtcgg acgagatgcc aagagcttgc
     1081 aagccgcgga gatgaaatcg ctccagcgtt tccccgccgc ccagcagcag cgcactgaac
     1141 aacagcagca ggaatgaaac accgagtgcg atccaaccgg gcgagcgttg acgcaacgcc
     1201 acaatcgcga tccaaagcgc gctcccgcca accaaaatga cgagcccagc gcgagagaga
     1261 ttcaagacca agccggcaac taacagggca aggccgggga tccacataac ccaacgcttt
     1321 cttccaaagc gcaaatcgtc ctgcgcgcag gcgagcagca cgatcgcgct gatgccgaag
     1381 agatcggcgg tctggttgcg attgggaaac gggccgaagc cgcgcggatt attccagaac
     1441 gctggtgaca tatgcgccat gtagaacccg acgctgaacg ccgcgagcac gacaatgccg
     1501 acgacgaaca ggcgaagctg ccagcgcacc gcgtgaagat cgacatcttg cgtgctgacc
     1561 aagtacagcc agcttactcc cgcgaccaag ctgatcagac acgttgtagt gatccacggc
     1621 tggggtgaga cggtcgacgg caaggcgatt ttgaattcgt tcgccagcgt cgtgcgccat
     1681 ggcgggatga agaaccagcc agagggcaga aatgcgattg cggccagcac gatgagcgcg
     1741 agcaagacac cgttgattgc atggccgagc gacacgcgcg gcggtcgcac cagcagaact
     1801 aatccaagca gcgtgacgac aattccttcg gcccacttct gcgtgctgcc gccgacgaaa
     1861 caggccagca ccggcagcag cgcgatggcg ctgttgctta gccaggcagt ctggccatcg
     1921 ccttgacgcc tgcgccggtg ctgagagtcc gacggctgat ctgccatcac gtgcgcgcga
     1981 aagttttggt atgcgaatcg ctgctaagct cgacaatcgc tgagcgaaga cgacgggctg
     2041 tcatgcggac gacgagctta gcacgagttg aagccgcgcg cgcgtcgggt acgctcgggg
     2101 cgctctcaaa gtcgccatga tcacgcaacg cacggaaggt ctctaccgtc tcttcctgct
     2161 ctgccaaatc gtgatcgtgg ccgcgctttt ctggctcggc gtctggatca tcgtcacgtt
     2221 ctatgcgccc ggcgccggcg tcgagttcac ctggcggcgc tacagcatct actgtggctg
     2281 catcgtgctc ggcatctgga tcgaatcgct ctcccgcagc gggtcgcgca attacttcct
     2341 gcaaaacgag ctcctcctgc agcatcggct cagcctccgc caaaccatcg ccggcatcgg
     2401 cgtgcttgtg ttctacctca tcggcacgaa ggacgccttc atttcgcgcg tcttcttctt
     2461 caactttgtg ccgtggttgt atgtcgcgct tcttttctct catcattatc tgccgtcgct
     2521 gttggcgcgc gcattcttca ccctgcgtga tgaaaagaca cttctcgtcg ggtcaactgc
     2581 gcgcgccgcg cacctccgca cctggctgca ccgcaaagcc gaaatcggcc tgcgcaccgt
     2641 cggcttggtt tgcgatgacg acatggagaa tcacgactca agcatcccgg ttctcggcaa
     2701 accgagtgag ctggagaaaa tcatccccgc gcgcggcatc acccagatca tcctgctcga
     2761 gttcccgctc ttcaccgaag ccaaccgcaa catcattcag atctgcgatc ggctgggaat
     2821 tcgactgctc atcgtgagcg acctcgaaga gaagctccgc catcccgtca cgcattttga
     2881 agatgatggc ttgcgcttca tcgctttgcg cgaggaaccg ctcgaaaatc cgctcaaccg
     2941 tttttttaaa cgtgctatcg acctcgcgat ttcaatccca gtcatgttct tcattttccc
     3001 aatttgcgcg gcggcagtgt ggatcgcgca acgcttccag tcccgcggcc cgctctttca
     3061 tgtgcagacg cgcgccggca tgcaaaatcg acaattcaaa atttggaagt tccgcaccat
     3121 gcacgtgagc aacggcgaca tcgcacgcca ggcgcaccat gatgatgagc gcgtttatcc
     3181 gctcgggaaa tggttccgca aattgagcat cgatgaggtc ccgcagttct ggaacgtcct
     3241 ccgcggcgac atgagcattg tcggcccacg cccgcatctc atcgagcaca acaatcagtt
     3301 ctcgcgcctg ctcgagaatt accacgtccg cgcgttcgtc aaaccgggca tcaccggcct
     3361 cgcacaggtg cgcggtttcc ggggcgaagc gcgcaacaat tccgacatcg ccaaccgcgt
     3421 cgcgtgcgac attgaatatc tcgagaactg gaatctctcg ctcgagtgcg gaatcatcct
     3481 gcgaaccttc gcgcaattga tcgttccccc gcggactgcc tactaatccc gcgcctcgag
     3541 cgcctgcagg cggctgcgat ggatcgcgag cagcgaatcg cgcggcgctt ccggaccaca
     3601 tggaaccaaa gtccggccgt ggtagagcgc gaagtctcgt tcccgcagaa aatcgggaat
     3661 cgcgccatcg ctgtagccat cgaggtaaat cactttgacg cgttgctcct cgaacaatct
     3721 ggcggctcca cgcagcaccg gcagctcgtg accctcgacg tcgattttca caaccaggtc
     3781 agtctgcggc agcgacagcg cgtccagccg ctcacaatga actttcattt ccgcggcctc
     3841 gcgcgcgctg acccggaaca cgcccgaagt cacgccagaa gtaaaaccga gctcaccgga
     3901 tcggtccgac attgcaatgt tcaacacctg gaccgattcg cccacggaat cgcgcagccg
     3961 ttctgcggtc tgccgattcg gctcgatcgc gacgtagttt gtgtctggcg agatattccg
     4021 cagcc
//
LOCUS       DASTDG010000055         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1519731, whole genome shotgun sequence.
ACCESSION   DASTDG010000055 DASTDG010000000
VERSION     DASTDG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519731"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="VFO30_01705"
     CDS             complement(<1..1938)
                     /locus_tag="VFO30_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418030.1"
                     /translation="MSSIRNPIVIALLLFFAIAEARAGEFHSILRWRNVGPYRGGRTR
                     AICGVPSQPNVFYMAPVNGGVFKSIDYGRAWQPIFDDQPTASIGAIAVSISNPNVIYV
                     GSGEGLHRPDLSVGDGVYKSTDAGKAWTHLGLRDGQQVAQLAVDPKNPDRVFVAVAGH
                     PYGPNEERGVYRSLDGGKRFEKVLYRDENVGASDVLIDPSNPQIVYAALWESREGPWE
                     NGVFDGDGGSISKSTDGGKTWRQLTKGLPANIVQANIAIAPSAPKTLFAAVRTKTISK
                     LYCSDDGGETWSGTTDDPRPGLGIGGGDLPVVRFDPKNPQIVYSASIVCWKSTDGGKT
                     WEGWRGAPGGDDYQNVWINPNNPDIILLGSDQGAIVTVNGGKSWSSWYNQPTAQLYHV
                     SADNAFPYRLYSGQQESGSVGIKSRGDQGEITFRDWHPVAAEEYGYVVADPLDPDIII
                     GGKLTRFERRTGQAQNIVPVPVQTEGFRMLRTEPVVFSPLDPHLLFFAGNTLWQTRDR
                     GDHWEKISPDLSRPNYELPASIGKYKEDATKQAHRRGVIYTVAASPLDANRIWCGTDD
                     GLIHLTTDGGKTWTNVTPPNISAWQKISLIEAGHFDSNTAYAAVNTMRIDDLRPHIFA
                     TRDSGKTWREIANGIPTGQVVN"
     gene            2148..2801
                     /locus_tag="VFO30_01710"
     CDS             2148..2801
                     /locus_tag="VFO30_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9418031.1"
                     /translation="MLSAVIFDLDGVLADSETWWNEIDAKLLAEHGVTYHGEYHQNVV
                     GVSYRLAVEFYKKAFGLSAPTEEMMRRRGEIATELFADRVGLFPYVKEVLEELWQMKL
                     HLAVATSSVSASARPFLGRHQLTGFFEVIVTGEEVERGKPAPDIYVCAAEKLGIPADA
                     CLVVEDALPGVAAGKAANMRVAAIPDRRFVDPAEYEKQADYLLGSLKELPQLVRKEL"
     gene            complement(2840..3193)
                     /locus_tag="VFO30_01715"
     CDS             complement(2840..3193)
                     /locus_tag="VFO30_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418032.1"
                     /translation="MRRKRHRSVEPEFHHNVYVVLLSKAALKDLSILRRNPARDPSKP
                     AIYVGMTGLPVDHRFENHKNGYKSARLVRKYGVRLLPELFEHLNPMPYEYAVQMEKDL
                     ADDLRAQGYAVCGGT"
     gene            complement(3545..4186)
                     /locus_tag="VFO30_01720"
     CDS             complement(3545..4186)
                     /locus_tag="VFO30_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9418033.1"
                     /translation="MIRFQVRKKNPDIHARSRVLVLVDESNVGSSVRTAGRGLDWLKL
                     RDFLAGPDTERDLIEMVVYAGLPPAIPTWQDERDKKNKFVHWLRSNGFMVVTKDGAPA
                     EEGHYKANVDVMMAIDALELSMEMRPDVVILVTGDADFAYLAIKLRRHGIRVEVASVA
                     TNLGNMLRSAANDVIDLAPLFRTFEIKVQDAPAISHPAQSASGGRRRRPPIRP"
BASE COUNT          907 a         1294 c         1165 g          972 t
ORIGIN      
        1 gttgacgacc tggccggtcg gtatgccgtt cgcgatctcc ctccacgttt tgccgctgtc
       61 gcgcgtcgca aaaatgtgcg gccgcagatc gtcgattcgc atcgtgttga ccgccgcgta
      121 agcagtgttc gaatcgaaat gtcccgcttc gattagcgaa atcttctgcc acgccgaaat
      181 gttcggcggc gtcacgttgg tccaggtttt tccgccgtcg gttgtcaggt gaatcaaacc
      241 gtcgtcggtc ccgcaccaga tccggttcgc gtcgagcggc gacgccgcaa cagtgtaaat
      301 cacgccgcgt cgatgagcct gctttgttgc gtcctctttg tattttccga tgctggccgg
      361 cagctcgtaa ttcggtcgcg acaaatcggg actgattttt tcccagtgat cgccacgatc
      421 gcgtgtttgc caaagcgtat ttcccgcgaa gaagagcaaa tgtggatcga gcggcgaaaa
      481 gacaaccggc tcggttcgca acatccggaa gccttctgtt tggaccggca ccggcactat
      541 attttgcgcc tgaccggtgc ggcgctcgaa tcgcgtcagc ttaccgccaa tgatgatgtc
      601 cgggtcgagc ggatcggcaa cgacgtaacc atattcctct gccgcgaccg ggtgccaatc
      661 acggaaggtg atctcgccct gatcaccgcg gcttttgatt ccaacagaac cgctttcctg
      721 ttggccgctg taaagccggt acggaaacgc gttgtccgcg ctgacgtgat acaactgcgc
      781 cgtgggctgg ttataccacg agctccagct cttgccgccg ttcaccgtaa cgatcgcgcc
      841 ctggtccgaa ccgagaagaa tgatgtccgg attattcgga ttgatccaaa cgttttgata
      901 atcgtcgccg ccgggtgcgc cgcgccagcc ctcccaggtt ttaccgccgt ccgttgattt
      961 ccaacaaacg atgctcgctg agtacacgat ctgcggattt tttggatcga accgcaccac
     1021 cgggagatca ccaccgccga ttccaagtcc gggccgcgga tcgtctgttg tgccgctcca
     1081 ggtttcgccg ccgtcgtccg agcaataaag tttggagatc gtctttgtcc ggaccgcagc
     1141 gaacaatgtt ttcggcgcgc ttggcgcaat cgcgatgttt gcctggacga tgttcgccgg
     1201 tagccctttg gttagttggc gccacgtctt gccgccgtcg gtggatttag aaatgctccc
     1261 accgtctccg tcgaacaccc cgttttccca tggtccttcg cgcgattccc acagcgcggc
     1321 gtaaacaatt tgcggattac tcggatcgat caggacatcg ctcgcgccaa cgttctcatc
     1381 acgataaaga actttttcga acctcttgcc gccatcgagc gatcgataaa cgccacgttc
     1441 ttcgttgggt ccgtacggat gaccggcaac ggccacaaaa acgcggtccg gatttttggg
     1501 atcgacagcg agctgcgcga cctgctgacc gtcgcgcaat ccaagatggg tccacgcctt
     1561 gcccgcgtca gtggatttat aaacaccgtc gccgaccgaa agatccggcc gatgcaatcc
     1621 ttcgccgctg ccaacgtaaa tcacgttcgg atttgaaatc gaaaccgcga tcgcaccgat
     1681 cgatgccgtc ggctgatcgt cgaaaatcgg ctgccacgcg cgcccgtaat cgatcgattt
     1741 gaaaacgccg ccgttcaccg gtgccatgta gaaaacattc ggctgcgacg gcacaccgca
     1801 gatcgcacgc gtccgcccgc cgcggtaagg tccaacgttc ctccagcgca aaatcgaatg
     1861 gaactctccc gcgcgcgctt cagcgattgc aaaaaataag agtaaggcga tgacgatggg
     1921 attgcgaatc gaactcatgg catgaaccgg ccactctctt ccgctgttcc gaaattcgca
     1981 acgcgcgttc tcctcgccgc cgaggtacgg gagattcacc caatcgcttt cgaacgccgg
     2041 tccgatccgc cagtcgcacc gactcatcgc gtggcgaact cgggccgctt gaggtcaacc
     2101 gcccctaccg ataacctttc aacccttcaa catttcaatc ctctgccatg ctctctgctg
     2161 taatcttcga tctggacggc gtgctcgccg actccgagac gtggtggaat gaaatcgacg
     2221 cgaaattact cgctgagcac ggcgtgacat atcatggcga atatcaccag aacgtcgtcg
     2281 gggtgagcta ccgcctcgcg gtcgagtttt acaagaaagc atttggcctc tctgcgccca
     2341 ctgaggaaat gatgcggcgt cgcggtgaga tcgcgacgga gttatttgcc gatcgggtcg
     2401 ggctgtttcc ttatgtcaaa gaagtgttgg aagaactgtg gcaaatgaaa ctgcatctgg
     2461 ccgtagccac gagctcggtc agcgcgtctg cgcgaccctt tttaggtcga catcagctga
     2521 ccggattctt cgaggtcatt gttaccggcg aagaagttga acgcggcaag cccgcgcccg
     2581 acatctatgt gtgcgctgcg gaaaaacttg gaatccctgc tgacgcatgc cttgtggttg
     2641 aggacgcgct tcccggcgtc gctgcgggga aggcggcgaa catgcgcgtc gctgcgatcc
     2701 cagaccgccg cttcgtcgat cccgctgagt atgaaaaaca agcggactac ctccttggca
     2761 gtttgaaaga attaccccag cttgttcgca aggagctgta gccgcgggcg ttgaccgcgc
     2821 cgagcaactt gtagattcct caagttccgc cgcaaacggc ataaccttga gcgcgcaaat
     2881 cgtcggccag gtctttttcc atttgaacgg catattcgta cggcattggg ttcagatgtt
     2941 caaacaactc cggcaacaat cgaacaccat atttcctgac cagccgggcc gacttgtacc
     3001 cgtttttgtg attttcaaat cgatgatcga ccggcaatcc ggtcattcca acgtagatgg
     3061 cgggtttgga tggatcgcgc gccggattgc gccgcaggat cgacaaatcc tttagcgcag
     3121 ctttggaaag tagaactacg taaacgttat gatggaattc gggctcgacc gatcgatgcc
     3181 gcttacgacg cacctgacgc tttaactcgg agagtaatga caggcaactg atggccgagc
     3241 gctcccgggg agcctggcta caatctctgc gtgtaatctg ttgtagccgc tgccgacgag
     3301 tgcggccaca aacttttagt agcaactatt cagccgcaga attgccgctt gccgcgctac
     3361 gacaacgtta tttcgccgac ttcttcttct tcgcgaggac gaggcgctca gtttttttac
     3421 ggcgccttgc gcgtttgtta acgttgtctc tgcctttgtt gtgtcccata gggtctaaca
     3481 gtcacctact cccgcggtct cgtcacctcg cagttttcac gaaagaccag aagcaactgc
     3541 tagtctatgg acgaattgga ggccggcgtc tgcgtccacc tgaggcggac tgcgccggat
     3601 gtgatatcgc cggcgcgtcc tgaactttta tctcgaacgt acgaaacagg ggagccagat
     3661 cgatcacgtc gttcgcagct gatctcaaca tgttccccag atttgtagca accgacgcga
     3721 cttctacgcg gatcccgtgt cggcgcagct tgatggcgag ataagcaaaa tcggcgtcac
     3781 cggttacgag aatcacaaca tcaggccgca tctccatgga aagctccagc gcgtcgatcg
     3841 ccatcatcac gtccacgttc gccttataat gaccttcctc ggcaggggcc ccatctttgg
     3901 tcacgaccat gaatccgttg gagcgcagcc aatggacgaa cttgtttttc ttgtcgcgct
     3961 catcctgcca cgtcgggatg gccggcggca atccggcata gaccaccatc tcgattagat
     4021 cgcgttccgt gtctggcccg gccagaaaat cgcgcaactt tagccaatcg agcccacgtc
     4081 ccgcagtccg cacagaactg ccgacgttcg attcatccac cagcaccaac actcgcgaac
     4141 gagcgtgaat atcgggattt ttctttcgca cctggaaacg aatcaactac atgcgtgcaa
     4201 tgacgcgact ccaatttcta gtccaagcta gaccctgcga aatgtgtttc cgccgaaaat
     4261 gattgccagt ccaagcagga tcactgccac cggcaccagg ggagcgccgg tgatgtaggt
     4321 tagcaggacg aagagaat
//
LOCUS       DASTDG010000056         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_161444, whole genome shotgun sequence.
ACCESSION   DASTDG010000056 DASTDG010000000
VERSION     DASTDG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161444"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFO30_01725"
     CDS             complement(<1..1026)
                     /locus_tag="VFO30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9418034.1"
                     /translation="MLPPNQVCLYPLAASLREAQTLRWKIIAFALAVLVGGFGASLFL
                     AQGLAKPVDRIVAGSVENLSRRRQAEKDLRETNRELEKTLRELKATQQQVIQQERLSA
                     IGQMASGIAHDFNNTLMPILGFADVLLENPVLLDDKQETRRCLEMLRTSAKDAANVVA
                     RLREFYRPVESDEQFPIVDLARIVQQAVSLTEPKWRHQAQARGATIKVSAEAKAFPIV
                     AGEESALREVLTNLIFNSVDAMPNGGEISLETAIENDRAVIRVSDTGTGMTETVRQRC
                     LEPFFSTKGEHGTGLGLSMVYGIIQRHRGELQIQSELGQGTTFLISLPLAQSSSIPSA
                     APPVVSKP"
     gene            complement(1013..1897)
                     /locus_tag="VFO30_01730"
     CDS             complement(1013..1897)
                     /locus_tag="VFO30_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418035.1"
                     /translation="MRENKSPRFPILNISGALNFRAKLLLAMMLVVFAATSATLYVAE
                     NNLRSNQQKLLDAQFEGQLRSFLAVQDLQSNAIAEKCRTLSRSVRLRAALEERDVDDL
                     YANALTELQGVFEPTRAAADPEPGLVRASFFRFFDSQGVVLPPGQHPAGLFEQSALDA
                     TLAPLGRAPADIDEQSIGFIALGRGSRLTVLREIVVTKIRDWNGSILGALVIGFPMAE
                     MRSQDAQSGSTIGSGIWLNQKLYIDGISTPDRRLLAQRVGAAAKASPAGNFVVDLENG
                     PHLLFYKALDPETKFAPA"
     gene            complement(1884..2537)
                     /locus_tag="VFO30_01735"
     CDS             complement(1884..2537)
                     /locus_tag="VFO30_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET9418036.1"
                     /translation="MHPRVVIAVCAIGLASPSFAGNDAAVEGVVQLPAPTPERPLDQR
                     YRTSEAVLAPSNPPAAVVYLEGDFSAATEPAAKTTAEMSQKNMQFSPDLVAVHIGTAV
                     AFPNLDDTYHNVFSYSKTKRFDLGRYRKDERPGTVIFDKPGVVTVHCEIHDRMRGTIL
                     VLETPYFQKTDTFGHYRMDHLPAGHFLLKAWVSDTDVRTQQVDLKQGAALHVDFAGK"
     gene            2663..>3051
                     /locus_tag="VFO30_01740"
     CDS             2663..>3051
                     /locus_tag="VFO30_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418037.1"
                     /translation="MHRRSSSLFLFRFVLIFFAPQLFAASSVQERADRFLKFANSGYQ
                     ALYRVNSEAQWLAVTDVTPEHDAAAAATGKAYAAFNGNPAIITEARELLTNDKELTEL
                     TVRQLKQLLLNAAEGPMTNPELVAKRV"
BASE COUNT          613 a          895 c          908 g          635 t
ORIGIN      
        1 cggtttgcta acaaccggag gcgccgcgga cggaatcgac gagctctgcg caagcggcag
       61 actgatgagg aaagttgtgc cttggccgag ctccgactga atctgcagct cgccgcgatg
      121 tcgttgaatg atgccgtaga ccatcgacaa accgagcccg gtcccgtgtt cacccttggt
      181 tgaaaagaac ggttcaaggc agcgctgacg cacggtttcc gtcatgccgg tgcccgtgtc
      241 gctcacgcga atgacggcgc ggtcgttttc aatcgccgtt tcgagcgaaa tttcgccgcc
      301 attcggcatt gcatcgacgg agttaaagat gaggtttgtg aggacttcac gcagcgcgga
      361 ttcttcgcca gcgacaatcg gaaacgcctt cgcctcggcg ctgaccttga ttgttgcgcc
      421 acgcgcttgc gcctgatgcc gccacttggg ctcagtaagt gagacggcct gctgcacgat
      481 ccgggcaaga tcgacaattg gaaattgctc gtcgctctca accggccggt aaaattcgcg
      541 caggcgtgcg acaacgttgg cggcgtcttt agccgaagtg cgcagcatct cgagacaacg
      601 gcgcgtttct tgtttgtcat cgagcaggac gggattctcg agcaggacgt cggcgaaacc
      661 gaggatcggc atgagcgtgt tgttgaagtc gtgcgcgatg ccgctggcca tctgcccgat
      721 cgcactgagc cgctcctgtt gaatcacctg ctgctgcgtc gctttcagtt cacgcagcgt
      781 tttttccagc tcgcgatttg tctcgcgcaa atccttctcg gcctgccgtc tgcggctcag
      841 gttttccact gaaccggcga caatcctgtc gaccggtttt gccagtccct gcgcgaggaa
      901 cagactggca ccgaacccgc cgaccaacac ggccagagcg aaggcgatga ttttccagcg
      961 cagcgtttgc gcttctcgca gcgacgcggc caaggggtat aggcaaacct ggttaggcgg
     1021 gagcaaattt cgtttctggg tcgagtgcct tgtaaaacag caggtgcggc ccgttttcga
     1081 gatcgacaac gaaattgccc gccggggatg cctttgctgc cgcgccgaca cgctgcgcca
     1141 ggagacggcg gtcgggcgtg ctaatcccgt cgatgtaaag cttttggttg agccagatgc
     1201 cgcttccgat cgtgctgccg ctttgcgcat cctgactgcg catttcggcc atcggaaaac
     1261 cgatcacgag cgctcctaaa atgcttccgt tccagtcgcg aatcttcgtg acgacgatct
     1321 cgcgcaacac cgttaggcga ctaccgcgcc ccaaggcaat gaagccgatg ctttgctcat
     1381 caatgtcggc cggcgcgcga ccgagcggcg cgagcgttgc atccaatgct gactgctcga
     1441 acagtccggc gggatgttgg ccgggcggca aaaccacgcc ttgggaatcg aaaaatcgaa
     1501 agaacgaagc gcgcaccaga cccggctcgg gatcggcggc cgcgcgcgtg ggctcgaaca
     1561 cgccttgcaa ttcggtcagt gcattggcgt agagatcgtc gacatctcgt tcctcaagcg
     1621 cggcgcgcaa tctgacggag cgagacagcg ttcggcattt ctccgcgata gcgttcgatt
     1681 gcaagtcttg gacggcaaga aacgagcgca gttggccctc gaactgcgcg tccaggagtt
     1741 tttgctgatt ggagcggaga ttgttttccg cgacatagag cgtcgcactc gtcgcggcga
     1801 agacgacgag catcatcgcc agcagcaact tggcccggaa atttagcgcg ccggaaatgt
     1861 tcaaaatggg gaaccgcggc gatttatttt cccgcaaaat cgacgtgcag cgccgcgcct
     1921 tgtttcaggt ccacctgttg cgtgcggacg tctgtgtcac taacccaggc tttaagcagg
     1981 aaatggccgg ccggcagatg atccatccga taatggccga acgtgtcggt cttttggaag
     2041 tacggcgtct ccaagaccag aatcgtgccc cgcattcgat cgtgaatctc gcaatgcacc
     2101 gtcacgaccc cgggcttgtc gaagatcacc gtgccgggcc gttcatcttt gcgatagcgg
     2161 ccgagatcga agcgcttcgt cttcgaatag gagaaaacgt tgtggtaagt gtcatcgaga
     2221 ttcggaaacg cgaccgcggt gccgatgtga actgcgacca gatcgggaga gaattgcatg
     2281 ttcttctgcg acatctcggc agtcgttttc gccgcaggtt cggttgcggc tgagaaatcg
     2341 ccctccaggt aaactactgc tgccggcgga ttgctcggcg cgagcaccgc ctcgctcgtt
     2401 cgatatcgtt gatcgagcgg acgttccggc gttggcgcag gcagttgtac tacgccttcg
     2461 actgcggcgt cgtttccggc aaaactgggt gacgccagtc caatagcgca aacagcgatg
     2521 accaccctgg gatgcacact ggatcagtta aaagcgagaa aggtgccaga tccgacggcc
     2581 gtccgcgagg tgccagcacc tgcaggcagc gacgcccgcc gcgcgcttgt cctgcaatgt
     2641 tggcttcgcg taacgtgcga ccatgcatcg tcgatcgtct tcactcttcc tcttccgctt
     2701 tgtcctaatc ttcttcgcgc cgcagctctt cgctgcttct tctgttcagg aacgcgctga
     2761 tcgtttcctg aagtttgcga actccggtta tcaggcgctt tatcgagtaa acagcgaagc
     2821 gcagtggctc gcggtcacgg atgtaacgcc ggaacacgac gccgcagctg cggcgaccgg
     2881 caaagcttac gcggccttca atggtaatcc ggcgatcatc accgaagcgc gcgagctgct
     2941 aacgaacgac aaagaattaa ccgagctaac ggtgcgccag ttgaaacagc tgctactaaa
     3001 cgcagctgaa ggcccgatga cgaatcccga gcttgtggcg aaacgcgtgg a
//
LOCUS       DASTDG010000057         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_94417, whole genome shotgun sequence.
ACCESSION   DASTDG010000057 DASTDG010000000
VERSION     DASTDG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94417"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..688
                     /locus_tag="VFO30_01745"
     CDS             167..688
                     /locus_tag="VFO30_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418038.1"
                     /translation="MFATIYLPNFYLQAATRHQPELREKPVGLIEEQENKPVIIQLNA
                     EAESAGIRKGMTPSQALARSLQVVIKVRAPAQEKALAEILLQHAFTLSPYVEATAPGV
                     CTIQFTDDRDLGRKVECVIHRLIDCDIVAQAGIAPLPDTSFLAAHLARPVLQIADAKE
                     FLAPLPIETLAIA"
     gene            complement(786..1442)
                     /locus_tag="VFO30_01750"
     CDS             complement(786..1442)
                     /locus_tag="VFO30_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET9418039.1"
                     /translation="MEKLRPWIAEFVGTFALIFVGVGAIKTAGHDVLGVALAHGLTIA
                     AFVSATLHISGGQLNPAVTFGLLCGGHMTLPNALRYWSAQLLGGLSAALICLGLFGRD
                     VVVTGTPQLAINLSPWQGILVEAILTFFLVFVVYGTAVDERGRRVAGFAIGSTITLDI
                     LFGGPLTGAAMNPARVFGPAVAANFWHDHYVYWIGPFLGAAAGGLVYSLLMERHAVER
                     "
     gene            complement(1559..>2961)
                     /locus_tag="VFO30_01755"
     CDS             complement(1559..>2961)
                     /locus_tag="VFO30_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418040.1"
                     /translation="QLGHASDAEALYQEIISKYPEREEGKEARYQRLINIYNSDASTL
                     PPEVDEFLATNPTTERADQAKLLKAEALYKQQNYQRAAPIYAELRAAQLSPKLRAECA
                     YKLGWCYIQLKDTAGIVEAFTYFAQAFPDSPQAPAALAQRALVYQQDKNYDAALADWN
                     TVLMKYPGAREREDALHNKALILGQQQNLKGMVDTFRQLLKEFPKTAAAARAQYYIGK
                     AAFELKDYKTALAALNAARQLSKDQYYDLATVRIISCYFGMRDRAAATKEVDGFLATN
                     PKAVVPAEILEWLGIEYYNEKNYAAAERYLGLIGKIDNPQVKPDFWFYLGDAAAKQQK
                     FEESENAFEKYLAASTDPGGKAKVLLALGAVKIAAHKADDAQRIAEQIMLLQPEGRVN
                     AEARLLAGDVQFERGRFEDAGKAFMGVALLYDDPAITPRALQKASVAYQRAGKSEEAD
                     RAARELRQKYPNYAGG"
BASE COUNT          623 a          830 c          885 g          623 t
ORIGIN      
        1 gccgacgcag catggtgagc agcgcgcaat tgaaattcgt tctcgaaaat tcatggacgc
       61 tgaaggattt gaacgaggag aacgcggcgg cgcggtggcg gtttcgcgtg cagcgggcgc
      121 atctcagatc agagggcgga gatccgatga cagagatcag cagctgatgt ttgcgacgat
      181 ttatttgccc aatttttatt tgcaggcggc gacgcggcat cagccggagc tgcgcgaaaa
      241 gccggttggg ttgattgaag aacaggaaaa caaaccggtc attattcagc tcaatgcgga
      301 agcggaatca gccgggattc gcaaaggcat gacgccgagc caggcattgg cgcggtcgtt
      361 acaggtggtg atcaaagtgc gtgcgccggc gcaggaaaaa gcgctggcgg agattttgct
      421 gcagcacgcg ttcacgttat cgccctacgt ggaagcgact gcacccggtg tttgcacaat
      481 ccagtttact gacgaccgcg atttgggccg gaaagttgaa tgcgtgattc atcggttgat
      541 tgactgcgac attgtcgcgc aagcggggat tgcgcctttg ccggacacca gctttctggc
      601 ggcgcatctc gcccgccccg tattgcaaat tgctgacgcg aaagaattcc tcgcgccttt
      661 accgatcgag acgctggcga tcgcgtaaga tcgacaatta gtccatctgt agccaccggc
      721 ctgtggccgg tttaaacaaa aataagacgg ctcacagagc cgtggctaca ggacacctct
      781 acaggttatc tctccaccgc gtggcgctcc attaagagac tgtaaaccag accgccggca
      841 gcagcgccga ggaatgggcc gatccagtaa acataatggt cgtgccagaa attcgcggcc
      901 acggcgggac cgaagacgcg cgccggattc atcgccgccc ccgtgagcgg gccgccgaaa
      961 aggatgtcga gagtgattgt cgatccaatg gcaaaaccgg cgacgcgccg tccgcgttca
     1021 tccacggcgg taccgtaaac cacgaacacg agaaagaagg tcaggatcgc ttcaaccaaa
     1081 attccctgcc acgggctcag gttaatcgcg agttgcggtg tgccggtgac aacgacatcg
     1141 cgtccgaaaa gtcccaagca gatcagcgcg gcgctcaagc ccccgagcaa ttgggcgctc
     1201 cagtaacgca gcgcattcgg caacgtcatg tgaccaccgc agagcagccc gaatgtgacc
     1261 gcgggattca gttggccgcc ggaaatgtgg agcgtcgcag aaacgaacgc cgcgatcgtc
     1321 agtccgtgcg cgagcgcgac gccgagcaca tcgtgaccgg cggttttaat cgcgccgaca
     1381 ccgacaaaga tgagtgcgaa agtgccgacg aattcagcga tccatggacg gagtttttcc
     1441 atactcgaat gcaatcaaat tgcattcgca gtgacaagtg atgagtgacg agtgacgaga
     1501 gctggaacca gaacagcaat cacttcgtca tttgttacct gtcactcgtc gctggctttt
     1561 agccgccggc atagttcggg tatttctgcc ggagctcgcg ggcggcgcgg tcggcttctt
     1621 cacttttgcc cgcgcgttga tacgcgacgg aagctttttg caaggcgcgc ggcgtgattg
     1681 cagggtcatc ataaagtaac gccacaccca taaaggcttt gccggcgtct tcgaatcggc
     1741 cgcgttcaaa ctgaacgtca ccagcgagaa gacgcgcttc cgcattcacg cgtccttccg
     1801 gttgcaggag catgatctgc tcggcgatcc tctgggcgtc atcggccttg tgcgcggcga
     1861 tcttcacggc accaagcgcg aggagcactt tggctttgcc gccgggatca gttgacgctg
     1921 ccaaatattt ttcgaacgcg ttttccgatt cctcgaactt ttgctgtttg gccgcggcat
     1981 cgccgaggta aaaccaaaaa tctggtttca cctgcggatt gtcgatcttg ccgatgagcc
     2041 ccaggtatct ctcggctgcg gcgtaattct tctcgttgta atactcgatg ccgagccatt
     2101 cgaggatctc ggcgggaaca accgccttcg gattggttgc gaggaaccca tctacctcct
     2161 tcgtcgccgc cgcacgatcg cgcattccaa aatagcagga gatgatgcga accgtcgcga
     2221 gatcgtagta ttgatccttg ctcaactgac gcgcggcgtt cagcgcggcc agtgcggtct
     2281 tgtaatcctt caactcaaaa gccgccttgc cgatgtaata ttgcgctcgc gctgcggccg
     2341 cggtttttgg aaattccttc aggagctgac ggaacgtgtc gaccatgccc ttgaggtttt
     2401 gttgctggcc aaggatgagc gccttgttgt gcaacgcgtc ttcgcgctcg cgcgcgcccg
     2461 ggtatttcat caggacggta ttccaatcag cgagtgcggc gtcgtaattt ttgtcctgct
     2521 gataaacgag cgcgcgctga gccaaggcgg ccggggcttg cgggctgtcg gggaacgcct
     2581 gcgcaaaata ggtgaacgcc tcgacaatac cggcggtgtc cttcaactga atgtaacacc
     2641 agccgagttt gtacgcacat tcggcgcgca gcttgggcga aagttgcgca gcgcgcagct
     2701 cggcgtaaat cggcgccgcc cgctggtaat tctgctgttt ataaagcgcc tcggccttga
     2761 gcaacttcgc ctggtcggcg cgctccgtcg tcggattcgt agcgaggaat tcgtccactt
     2821 cgggcggcag cgtggacgca tcggagttat aaatattgat caggcgctgg tagcgcgcct
     2881 ctttgccttc ctcgcgctcc gggtatttgg aaatgatttc ctgatagagc gcttcggcgt
     2941 cggacgcgtg cccgagctgg c
//
LOCUS       DASTDG010000058         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_328748, whole genome shotgun sequence.
ACCESSION   DASTDG010000058 DASTDG010000000
VERSION     DASTDG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328748"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFO30_01760"
     CDS             complement(<1..410)
                     /locus_tag="VFO30_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9418041.1"
                     /translation="MNQVRLLYGVLVKPVSSFRAIAQRPSILIPYVVFLLLCVPASLE
                     LVRKVDWNAVYQRQITQDSSGPANSDAPPSQNSQLISARVERVHKYGVVVYVLQNAVI
                     VLVAGFFFWLTFNALLGAGIRYSTVLSVVCYALAP"
     gene            complement(561..1304)
                     /locus_tag="VFO30_01765"
     CDS             complement(561..1304)
                     /locus_tag="VFO30_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9418042.1"
                     /translation="MATATAVPEQQQPQMSGFARAFNVLFSPKATFEDIVRKPSWIVP
                     IALLTIFSIGAGALISKKVDWASFIRAQSEKSAAFQQLSDDQKQQRVDIGAKFAPMAA
                     WIFAVCGPILFAVILTLVYWAAFNVISGAQLRFGVPFGIVSHSFMPSIFATILLIVVL
                     SMKSYGEVNPNTMLASNLAAYVPSDSPNWLISLGQSLDVFWIWTMALLVIGFKAANPR
                     KISSASAFGTVFGLWAIYVLVKVGWASMM"
     gene            complement(1436..>2193)
                     /locus_tag="VFO30_01770"
     CDS             complement(1436..>2193)
                     /locus_tag="VFO30_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9418043.1"
                     /translation="SSGEFSAGREDKRVAVDGVSFRLEKGETLAIVGESGSGKTTLAR
                     MLLRLVEPDSGEIELNGANLLAASGADLRRLRARMQMIFQDPFSSLDPRMAVRELIAE
                     PLSIHEPRLTRTQRAERVRDSLASVGLDASAANRMPHEFSGGQRQRIGIARALILRPA
                     LIVADEPVSALDVSVGAQIIELLARLQHELLLTYLIISHSLPVVAQLATRVAVMHEGR
                     FVEEGVAQQVLDHPSHPYTQSLLAAVPEVPAAN"
BASE COUNT          535 a          630 c          618 g          410 t
ORIGIN      
        1 ggagccagcg catagcaaac caccgagaga acggtggaat aacggattcc cgcccccagc
       61 agcgcgttga atgtaagcca aaaaaagaat cccgcgacca gaacgatgac cgcgttttgg
      121 aggacgtata cgactacacc atatttatgc acccgctcga cgcgcgccga gatcagctgg
      181 ctattttggc tcggcggagc gtcggagttc gccggcccgg aagaatcctg cgtgatctgg
      241 cgctgataca ctgcgttcca atcgaccttt cggacaagct cgagtgaagc cggcacgcaa
      301 agcaaaagga aaacgacgta cggaatcaat atcgacggcc gctgcgcgat cgcgcggaac
      361 gaggaaaccg gtttgaccaa tactccgtag agtaagcgaa cctggttcat ttggagatta
      421 acgggcagag ctttgctgca caacctgccg gcgcgggaaa cataccccaa cttcagtgca
      481 gcgcgtaaca actagcaaaa atttcgctta ttgagttaag gccactcgag ccgctagcaa
      541 atctccgcgc cccggcaagg ctacatcatc gacgcccagc cgaccttcac gagaacatag
      601 atcgcccaaa ggccaaatac agtgccgaaa gctgatgcgc tggagatctt acgcgggttc
      661 gcggctttaa aaccgattac gagcagggcc atcgtccaaa tccagaaaac gtcgagcgat
      721 tggccgagcg aaatgagcca gttcgggcta tccgatggga cgtacgccgc aagattgctc
      781 gcgagcatcg tgtttggatt cacttcgcca taactcttca ttgataagac cacgatcaaa
      841 aggattgtcg cgaaaatcga cggcatgaac gaatgagaca caatgccgaa gggcacccca
      901 aatcgaagct gcgctcccga gatcacatta aacgccgccc aatacaccag agttagaatc
      961 acggcaaaca gaatcggtcc gcagaccgca aaaatccaag ccgccatcgg tgcgaatttt
     1021 gcgccgatgt cgactcgctg ctgtttctga tcgtcggaga gttgttgaaa cgctgcagat
     1081 ttctcgctct gcgcgcgaat gaagcttgcc caatcaacct tcttgctgat cagcgccccg
     1141 gcgccgatgc taaaaattgt aagcagcgcg atcggaacga tccaactagg cttgcgaacg
     1201 atatcttcga atgtggcctt ggggctgaac agtacattga atgctcgcgc gaaaccgctc
     1261 atttgcggct gttgctgttc gggcacagca gtcgctgtag ccatagtggt gcctccagta
     1321 gactcacgct gcggattctc aaccgacctt gaccgtatta cgggcgactt ttgcagaagt
     1381 ttcacccgaa acgcaaggaa ttaattgtcg cgagtttggc gagacgcgag aagcttcaat
     1441 tcgctgcggg cacttcgggc acagcggcga gaagcgactg agtataagga tgcgacggat
     1501 gatcgagaac ctgttgcgcg acgccttcct cgacaaaacg tccttcgtgc attacggcaa
     1561 ctcgcgtggc taactgcgcc acgacgggta agctgtgcga aatgatcaga taggtgagca
     1621 agagctcgtg ctgcagcctg gcaagcagtt cgatgatttg cgcgccgacc gaaacatcaa
     1681 gcgccgagac cggctcatcc gcgacaatca acgcgggccg caggattagc gcgcgcgcga
     1741 tgccgatgcg ctggcgttgt ccgccagaga attcgtgcgg catgcgattc gccgcggagg
     1801 cgtcgagtcc gacggaagca agcgaatcgc gaacgcgctc cgctcgctgc gtgcgagtga
     1861 gccgcggctc gtgaatggag agcggctccg caattagttc cctgaccgcc atgcgcggat
     1921 cgagcgatga aaacggatct tgaaaaatca tctgcatccg cgcgcgcagg cgccgcaaat
     1981 ccgcaccgct ggcggccaga aggttagcgc cattcaattc gatttcgccg gagtccggct
     2041 cgaccaagcg aagcaacatg cgcgccagcg tcgtttttcc gctgccggat tcaccgacga
     2101 tcgcgagggt ctcgcctttt tcgagccgga atgagacgcc atctacggcg acgcgcttat
     2161 cctctctgcc ggccgagaat tcgccgctgg agc
//
LOCUS       DASTDG010000059         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1351514, whole genome shotgun sequence.
ACCESSION   DASTDG010000059 DASTDG010000000
VERSION     DASTDG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351514"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="VFO30_01775"
     CDS             complement(<1..2099)
                     /locus_tag="VFO30_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HET9418044.1"
                     /translation="MRTNSTPVKLLLPSLIVCQLACSLAALGQTVERVAQREVARRQA
                     AIPRGADALARGKRAMLDKNYTLAHDEFRTAVRLLPDAVTSGNLHDEALAGFCESGVK
                     LAEQKIAEGKYGEAEGILREVLDEHYSPNCGAASELLAHLRQPGYFNKTMGPKFIAKV
                     EQVKALLADADGYYNSGRYDLAFKKYEQVLALDPYNVAARRGEEKIDNTKYHYNEQAY
                     NETRARQMWAVEKGWEQPVHQYGQTVGPLGEAFQRDVTGTARITQKLNTIIIPKIEFR
                     DASIREAIDFLRQQAALNDPTTEGKKGVDIVLRLVPLGQVAPPPIPVEPAAAPGAAAA
                     PAGGAPETAPAAQTTPLPARPVVAPAAVAASAISPANARITLSLSQIPLGEALRYIAS
                     QAGLKVKVEPYAVSIIPISEQSNDLITKEYRVPPGFITTTVNVAPSVLNQPARTGAGA
                     AAAPAAPAVSAGTAKDTVESTGGHQLVGRETAKDFLESQGVPFPPGASANFLPQSSRL
                     IVRNTADNLELVDALVEQAAIAGPKQVEIESKFIEITQNNLKELGFDWLLGQFNIGNH
                     KVFGSGGTFGTHPAGNPADFPILGANGIPVGENPVSAGTRSGNLAISANALDALLFPT
                     TGATSLAPGIFGLAGVFTDPQFQVVVRALNQKKGVDLLSAPKVTTKSGQRAVIEIVRE
                     FRYPTTFTPPQVPSISS"
     gene            complement(2112..3173)
                     /locus_tag="VFO30_01780"
     CDS             complement(2112..3173)
                     /locus_tag="VFO30_01780"
                     /inference="COORDINATES: protein motif:HMM:NF042425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1099 family pilus-like system protein"
                     /protein_id="HET9418045.1"
                     /translation="MANGFAAIFNIELGELDPPAREAAIRLSLRAGTALAGMNWIRVN
                     YERVILVVAALFLLISAVSIWRYAATFSASFSGRHFAARPKPASPPGKARELMAAEEK
                     LHEPSQWAFRGRSGLFVPEKHFIGAEGVPVTLKTAEVHPPVPNEWFETYGLNIADSDI
                     LNADPDGDGFNNLEEWQGHTNPIEKSSHPDYLTQLKMKSFTEEPFRLIFSSWVADTYA
                     INTIDMREPTQFLKIGDIVAGTPFKLVKFTPKYTTNQYGTTVDVSELTLEQQDSKEQI
                     TLVKEQVAMSPESVVTFVYAWPCNQPAREFQIRKDQEFSLKPQEEIKYKLVDVQLNKA
                     VILNTKEPDKPIEIGLLTP"
     gene            complement(3234..4115)
                     /locus_tag="VFO30_01785"
     CDS             complement(3234..4115)
                     /locus_tag="VFO30_01785"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HET9418046.1"
                     /translation="MNWFKQNRWLGVFLSVFSALILLSLLFVWRARAGLAEATAGLDE
                     ALKELNRLERLDPFPNDVNYEKLKVHLDNYSGTLEKMKDALKTRMLPVAPLAPNEFQA
                     RLRQVTLAAADKARTNKVKLPPGFHMGFDEYTAALPNNAAAPLLGQELSQINLLVDYV
                     IDARVDSLISLRRAPLAEERAATTGGAATPQPAAPQTGPATGAPRILDRNIVDLTFFG
                     PSAAARRVLNQIASSSQQFYVVRTLYVRNEKGSGPPREEPGKAQATPTPAGALNFIVG
                     NEHIQVSARIEMLRFNF"
     gene            complement(4112..>4855)
                     /locus_tag="VFO30_01790"
     CDS             complement(4112..>4855)
                     /locus_tag="VFO30_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418047.1"
                     /translation="LGELVGLALRAVTTCPMELNLRPASVLRRQQLEKRRPFLAAAAA
                     CVILLLLGWGIFFVRAAQVTRGSIEQLQPKIGTMRAAERKFEPLRRQTAALDKMATPL
                     IAAVNERGYWVELLEDLNTRLPAADIWVTELIPLSGGRPVGVDEKRLSESGSNPVITA
                     TAATSTSKAPRSTGTVDAVLVRGLYMFNPKQEQLVVDYFRNLIGSPLFAVNASDQSRV
                     IKSTIPNNTEWAFPYELHLDLRKPLTLLP"
BASE COUNT          917 a         1423 c         1440 g         1075 t
ORIGIN      
        1 ctactgctaa tcgacggaac ctgcggcgga gtaaaggtgg tcgggtaacg aaattccctg
       61 acgatctcga tgaccgcgcg ctgaccgctc ttggtcgtga ctttcggtgc agaaaggaga
      121 tcgacacctt ttttctggtt caaggcgcgg actacgactt gaaattgtgg atcggtgaac
      181 acgccggcca atccgaaaat ccccggggca agtgaagttg ctcctgtggt tggaaacaag
      241 agcgcgtcga gtgcgtttgc gctgatggca agattgccgc tgcgagtccc agccgagacc
      301 ggattctcac cgactggaat tccattggca ccgaggatcg gaaaatccgc agggttcccg
      361 gcaggatgtg ttccgaaggt cccgccggaa ccaaagactt tgtgattgcc aatgttgaac
      421 tgcccgagga gccagtcgaa accgagctcc ttgagattgt tttgcgtgat ctcgatgaac
      481 ttcgactcga tctcaacttg tttgggtccg gcaatggcgg cttgttcgac caacgcatcc
      541 accaactcaa gattatcggc agtgttgcgg acgatcaggc gactgctctg cggcagaaaa
      601 ttcgcactcg cccccggcgg gaatggaacg ccctggctct cgagaaaatc cttggcagtc
      661 tcgcggccga caagctggtg tccaccggtc gattctactg tgtcctttgc cgtgccggcg
      721 gaaacggccg gcgccgcagg tgcggcagcg gcgcctgcgc cggttcgcgc cggctgattc
      781 aacaccgaag gcgcgacgtt gaccgtggtg gtaatgaagc cggggggcac gcggtactct
      841 ttcgtgatca gatcattgct ctgttcgctg atcggaatga tcgagacagc gtagggctca
      901 actttcactt ttaatcccgc ctggctggcg atgtagcgaa gcgcttcacc aagcggaatc
      961 tgactgagcg aaagggtgat gcgcgcattc gccggcgaaa tggctgatgc cgccacagcc
     1021 gcgggcgcaa ctacaggccg tgccggtaac ggcgtggttt gcgcggccgg tgcagtctcg
     1081 ggcgcaccgc cagccggtgc tgccgccgcg cccggagccg ccgccggttc aactggaata
     1141 ggtggaggcg ccacctgacc tagtgggacg aggcgtaaga caatgtcgac gcctttcttt
     1201 ccttctgtcg tcgggtcatt aagcgcggct tgttgtcgga ggaaatcgat cgcttcgcgt
     1261 atgctcgcgt cgcgaaattc gatctttggg atgatgatcg tgtttagctt ttgcgtgatg
     1321 cgagcggtac cggtgacgtc gcgctgaaaa gcttccccca acggcccgac ggtttgcccg
     1381 tactggtgga ctggttgctc ccaacctttc tcgaccgccc acatttgccg ggcgcgtgtt
     1441 tcgttgtagg cctgttcgtt atagtgatac ttcgtgttat cgatcttttc ctcaccgcgg
     1501 cgtgcggcca cgttgtaggg gtcgagcgcc aggacctgct cgtatttctt gaaggcgaga
     1561 tcgtatcggc cggagttgta gtagccgtcg gcatccgcga gcagcgcttt tacttgctcg
     1621 actttggcga tgaacttggg ccccatcgtt ttgttgaagt agccgggctg ccgcagatgc
     1681 gcgagcagct ccgacgcagc gccgcagttc ggactgtaat gttcatcaag cacttcgcgg
     1741 aggatccctt cagcttcgcc atatttgcct tcggcaatct tctgttcggc cagcttcaca
     1801 ccgctctcgc agaacccggc aagcgcttcg tcatgcagat tgccgctcgt aaccgcgtcc
     1861 ggcagcaagc gcaccgccgt gcgaaattcg tcgtgcgcga gcgtgtagtt cttgtcgagc
     1921 atggcgcgtt tgccgcgagc aagcgcatcg gcgccgcgcg ggatggcggc ctggcggcgc
     1981 gccacttcgc gctgcgcgac ccgttccacg gtctggccaa gcgccgcgag agaacaggca
     2041 agttggcaaa caatcaagct gggcagcagc agcttcacag gggtggagtt ggttcgcatg
     2101 gcgggtggag attatggagt aagcaggccg atttcgatcg gcttgtctgg ttccttggta
     2161 tttaggatca cagccttatt cagctgcacg tcgaccagct tatactttat ttcctcctgc
     2221 ggtttcaatg aaaattcttg gtcttttcgg atctgaaact cgcgcgccgg ctggttgcaa
     2281 ggccatgcgt aaacgaaagt gacgaccgac tcgggcgaca tcgccacttg ttctttcacc
     2341 agcgtgatct gttctttcga gtcctgctgc tccaacgtca gctccgacac atcaaccgtc
     2401 gtgccgtact ggttcgtcgt gtatttcggc gtgaacttga caagcttgaa cggcgtcccc
     2461 gcgacgatgt cgccgatctt gaggaattgg gtcggctctc tcatgtcgat cgtgttaatc
     2521 gcatacgtat cggccaccca ggaagagaaa atgagacgaa agggttcctc agtgaacgat
     2581 ttcatcttca gctgtgtgag ataatcgggg tgcgagcttt tctcgatcgg gttagtgtgc
     2641 ccctgccatt cttcgaggtt gttaaaaccg tcgccgtccg gatcagcgtt cagaatgtcg
     2701 gaatcagcga tgttcagtcc gtaggtttcg aaccattcgt taggcaccgg cggatgtact
     2761 tcggcggtct tgagcgtgac gggcacgccc tctgcgccga tgaaatgttt ttccggaacg
     2821 aatagtccgg agcgaccacg aaaggcccat tgtgacggct cgtgcagctt ctcctctgcc
     2881 gccatcaact cgcgtgcttt tccgggcggc gaggcaggct tgggccgcgc ggcaaaatgt
     2941 cgaccgctga agctcgcgct aaaggtcgcg gcgtaccgcc aaatcgagac cgcgcttatt
     3001 aggagaaaca acgccgcgac gaccagtatt acgcgctcat agtttacgcg gatccagttc
     3061 atgccagcga gcgccgtgcc tgccctgagc gacagtcgaa tggccgcctc gcgtgcaggt
     3121 ggatcgagct ctccgagctc gatgttaaaa atcgcggcga agccgtttgc catcgcccgc
     3181 ggagcggccg atccaccttg cgaggatgtt tattcatccg cttgatatcc cattcagaaa
     3241 ttgaatcgca gcatctcgat gcgcgccgag acctggatgt gctcgttgcc gacgatgaaa
     3301 ttcagtgcgc cggcgggagt tggtgtagcc tgcgctttgc ccggttcctc gcgcggcggt
     3361 ccactgccct tttcattgcg cacatacaac gtgcgcacga cgtaaaactg ctggctcgag
     3421 ctcgcgatct ggttcagcac tctgcgcgcg gcagccgaag gaccgaaaaa cgttagatcg
     3481 acaatgttac ggtcaaggat ccggggtgcg cccgtggccg ggcccgtttg cggcgcggct
     3541 ggctgcggcg tcgctgctcc gcctgtcgtg gcagcgcgct cttctgccaa cggcgcccgg
     3601 cgcaagctga tcaggctatc gacgcgcgca tcgattacat aatcgaccag taagttgatc
     3661 tgcgacagtt cctggccgag caaaggtgcg gctgcattgt taggcaacgc agccgtgtat
     3721 tcgtcaaagc ccatgtgaaa gccgggcggc aatttcactt tgttcgtgcg cgccttgtcc
     3781 gccgccgcaa gcgtgacctg gcgtaggcga gcctggaatt catttggcgc gagcggcgcg
     3841 actggaagca ttcgcgtctt cagcgcgtcc ttcatcttct cgagcgtgcc gctgtaatta
     3901 tcgagatgca ccttcagctt ctcgtaatta acgtcattcg gaaatgggtc gagccgctcg
     3961 agtcgattca gctcctttaa cgcctcgtcc aaaccggcgg tggcttcggc cagcccggcg
     4021 cgcgcacgcc acacaaaaag cagcgacagc agaatgagcg cgctaaaaac gctcaaaaaa
     4081 acaccgagcc agcgattttg tttaaaccag ttcacggcag cagggtcagc ggtttgcgta
     4141 aatcgaggtg gagctcgtaa gggaacgccc actccgtgtt gttcgggatg gttgatttga
     4201 tgactcggga ctgatcgctc gcgttgaccg cgaacaacgg tgacccgatc aaattgcgaa
     4261 agtaatcgac gaccagctgt tcctgcttgg gattgaacat gtagagcccg cgcaccagca
     4321 cggcgtcgac cgtcccggtg ctccgcggtg ctttacttgt cgatgttgcc gctgtcgccg
     4381 ttatcaccgg attcgatccc gattcgctga gccgcttttc gtcaacgccc accggtcttc
     4441 cgccggacaa cggaatcaat tcggtcaccc aaatgtctgc cgccggcaga cgcgtgttca
     4501 ggtcttcgag cagctcaacc cagtatccac gctcgttcac cgccgcgatc agcggcgtcg
     4561 ccattttatc gagtgccgcc gtttgtctcc gcaatggctc gaattttcgc tccgctgccc
     4621 gcatcgtccc gatcttcggc tgaagctgct cgatgctccc tcgggtgacc tgcgccgcgc
     4681 gcacgaaaaa aattccccag ccaaggagca gcaaaatcac gcacgccgcc gccgccgcta
     4741 agaacgggcg gcgcttttcc aattgctgtc tgcgaagaac gctcgctggc cgcaaattca
     4801 gttccatcgg gcaggtagtc actgcgcgca gcgccagccc gaccagttcg cctaa
//
LOCUS       DASTDG010000060         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_211616, whole genome shotgun sequence.
ACCESSION   DASTDG010000060 DASTDG010000000
VERSION     DASTDG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211616"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFO30_01795"
     CDS             complement(<1..228)
                     /locus_tag="VFO30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9418048.1"
                     /translation="MPATPEPYAKAGSLVTRSAGPTVIDIENIRKDYVMGEEIVHALR
                     GVSLQIRRGEYIAVMGPSGSGKSTLMNMLGCL"
     gene            complement(244..618)
                     /locus_tag="VFO30_01800"
     CDS             complement(244..618)
                     /locus_tag="VFO30_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9418049.1"
                     /translation="MNTTAIRSYKDLVVWQKGIALAKLVYQLTNNFPLAERFGLVAQM
                     RRAAISIPSNIAEGQARHTTSEFVQFISHAEGSVAEVDTQLILSMELNFCSQAVAEPS
                     FALIAELRRMLNVLRRRLIARQ"
     gene            complement(657..2018)
                     /locus_tag="VFO30_01805"
     CDS             complement(657..2018)
                     /locus_tag="VFO30_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9418050.1"
                     /translation="MHQPLSSSTVAPSSTRKQRARRKRRIIFGAIGLVAICGIVPFVL
                     SKREKPIPVTTEKALRRTIVQTVSATGKVQPETEVKISPEVAGEIIELPVQDGMIVKK
                     GDLLLRIKPDSYKALLEQQEAAISAAKATNLQQKATMMKAEQDFKRAEDLFNKKLISV
                     QEYNAAQAALDVAKNTFESSLHDIERAQAASSQARDQLSKTTIYSPIDGTITILNSKL
                     GERLVATGQFAGTEVMRVADLVHMQAVVDVNENDVVNVKLGDKADVKIDAYGERKFHG
                     TVQQIANTGKTTGAGTQEEVTNFEVKIRIDDHDVALRPSLSCTADIQTNMVKDAMAVP
                     MQSVTIRTGDSNLSPEEIEKRKTKMAAQEKDDSNAEVTNERLEKHVQKEEREKLAKVV
                     FLKEGAKAKLVKVVTGISDESYTEIKSGIKPGDEVISGSYSAISRKLKDGARVKIDKE
                     ALK"
     gene            complement(2129..>2921)
                     /gene="queG"
                     /locus_tag="VFO30_01810"
     CDS             complement(2129..>2921)
                     /gene="queG"
                     /locus_tag="VFO30_01810"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9418051.1"
                     /translation="AITGRVARYAWGDDYHELIAAKLDKIDNFLREFGGRQKCYVDTG
                     PVLERDFAAQAGIGWHGKSTMLINKRFGTWFFLAEILTTLELPPDEPARDRCGSCNRC
                     ITACPTGAITAPYRLDARRCISYLTIELKGAIPLELRPLIEDRIFGCDDCLEACPWNR
                     FAEVARESAFTARRSTIDFALRDYLALDEPRFRSIFRNSPIKRIKRRGFLRNVCVALG
                     NVGTAEDLPALKRAATDPEPLISEHAKWAIAEIEQRCGSITATAA"
BASE COUNT          554 a          871 c          781 g          715 t
ORIGIN      
        1 gagacagcca agcatgttca tcaacgtgga cttgccgcta ccgctcggcc ccatcacggc
       61 gatgtattcg ccgcgcctga tctgtaggga cacgccgcgt aacgcgtgca cgatctcttc
      121 acccatcacg taatctttcc tgatgttctc aatgtcgatg acggtcggcc ccgccgaccg
      181 agtgacgagt gacccggcct tcgcataagg ctccggcgtg gcaggcatgt gacgagtgac
      241 gagttattga cgtgcaatca gccttcgtcg cagcacattc aacattcggc gaagctctgc
      301 aataagggcg aatgagggct cagcgacagc ttgcgagcag aagttcagtt ccatgctcag
      361 gatcagctga gtgtccactt cagctacaga gccctccgca tgcgagataa actgaacgaa
      421 ctcactcgtc gtgtgccgcg cttgtccttc cgcaatgttt gaaggaattg aaatggccgc
      481 ccggcgcatt tgagcgacta acccgaatct ttctgctaaa ggaaaattat tcgtgagttg
      541 gtaaacgagt ttcgcgagcg cgatcccctt ctgccaaaca accaagtcct tgtagctgcg
      601 gatagcggtc gtattcatgt gagcgttgta gccaaacact tgtcactcgt cacccgtcac
      661 ttcaatgctt ccttgtcgat tttgaccctg gcgccgtctt ttaacttccg actaatcgcg
      721 ctgtagcttc cggaaatgac ttcgtcgcct ggtttgatac cgcttttaat ttccgtgtag
      781 gactcgtccg agattccggt tactaccttc accagcttcg ccttcgcgcc ttctttcagg
      841 aagacgacct tcgccagctt ctcgcgctct tctttctgca cgtgtttttc gaggcgctcg
      901 ttggtaactt cagcgttgct gtcgtctttt tcctgcgccg ccatctttgt cttccgcttt
      961 tcgatctcct cagggctgag gttgctgtcg ccggtgcgaa tggtgacgct ttgcatcggc
     1021 acggccatgg cgtctttgac catgttcgtc tgaatgtcgg ccgtgcagct taggcttggc
     1081 cgcagcgcga cgtcgtgatc gtcaatccga atcttcacct cgaaatttgt cacctcttcc
     1141 tgcgttcctg caccggtggt ctttcccgtg ttcgcgattt gctgaacggt gccgtggaac
     1201 ttacgctcgc cgtaagcgtc gatcttcacg tcggctttat cgcccagctt gacgttgacg
     1261 acgtcatttt catttacatc gacaactgct tgcatgtgca cgagatcagc cacccgcatt
     1321 acctcggtgc cggcaaattg gccggtggcg acgaggcgct cgccgagctt gctgttcaaa
     1381 atcgtgatcg ttccgtcaat cggcgaatag attgtcgttt tcgaaagctg gtcgcgcgcc
     1441 tggcttgatg cggcctgcgc ccgctcgatg tcgtgcaatg aactttcaaa agtgtttttc
     1501 gccacatcga gtgccgcctg tgcggcgttg tattcctgca ccgagatcag ctttttgttg
     1561 aacaggtcct ccgcgcgttt gaagtcctgc tccgccttca tcatagtcgc tttttgctgc
     1621 agattcgttg ccttcgcggc gctgatcgct gcttcctgct gctcgagcag cgccttgtag
     1681 gaatcgggtt taatccgaag cagcaggtcg cccttcttca cgatcatccc gtcctgcacc
     1741 ggcagctcga tgatttcgcc tgcgacctcc ggagaaatct tcacttctgt ttccggctgc
     1801 actttgccgg tcgcggagac ggtctgcaca atcgtcctgc gcagcgcttt ttccgtcgtc
     1861 accggaatcg gcttctcgcg tttggaaaga acaaagggaa caatgccgca tatagccacg
     1921 agaccaatgg cgccgaagat gatccgacgt ttgcgccgcg cgcgctgctt gcgggtagac
     1981 gacggcgcaa cagtggaaga gctcaggggc tgatgcataa gatgctgacg aagcggggga
     2041 aagcgcctga gattacctca gtttcggccg ttgcaaaggg aaacgcgttc aaccgacaat
     2101 tgagatgcgg taaaggcagt gtttggattc aagcagcggt cgctgtgatg ctcccgcagc
     2161 gctgctcaat ctccgctatt gcccactttg cgtgctctga gatgagcggc tccgggtcgg
     2221 tggcagctct tttcaacgcc ggtagatcct ccgccgtgcc gacgtttccc agtgccacgc
     2281 acacgttccg caaaaatccg cggcgcttaa ttcgtttgat cggcgaatta cgaaaaatgc
     2341 tgcgaaaccg cggctcgtcg agcgccaggt aatcgcgcag cgcgaaatcg atcgtcgatc
     2401 ttcgcgccgt gaacgcgctc tcccgggcca cctccgcaaa ccgattccac ggacaggcct
     2461 cgagacaatc atcgcaacca aaaatgcggt cttcgattag cgggcgaagc tcgagcggaa
     2521 tcgcgccttt tagttcaatc gtcaggtacg agatgcagcg ccgtgcgtcc aatctgtatg
     2581 gcgccgtgat cgcgccggtc gggcaagcag tgatgcagcg attacagctc ccgcaacgat
     2641 cgcgcgccgg ctcatccggc ggcagctcaa gcgtcgttag aatctccgca agaaaaaacc
     2701 acgttccgaa tcgcttgttg atcagcatcg tgctcttgcc atgccagccg attcccgctt
     2761 gcgcggcaaa atcgcgctct agcaccgggc cggtgtcgac gtaacatttt tggcgaccgc
     2821 cgaattcgcg caggaaattg tcgatcttgt ccaacttcgc ggcaatgagt tcatggtaat
     2881 cgtcgcccca cgcataccgc gcgactcgcc ctgtaatcgc g
//
LOCUS       DASTDG010000061         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_77013, whole genome shotgun sequence.
ACCESSION   DASTDG010000061 DASTDG010000000
VERSION     DASTDG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77013"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="glnA"
                     /locus_tag="VFO30_01815"
     CDS             complement(<1..1330)
                     /gene="glnA"
                     /locus_tag="VFO30_01815"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9418052.1"
                     /translation="MIKDNEVKLIDFKFTDLLGTWQHFTTTLTEYDEGIFTDGLGFDG
                     SSIRGWRVINASDMLVIPDATTAWIDIFNAEPTLSLICTIVDPITREPYDRDPRGVAE
                     KAEAYLQSTGIADTAYFGPEAEFFIFDDVRFSYSGNASFHRVDSMEGHWNSAREEFPN
                     LGYKIRPKEGYFPVSPMDSLQDIRNEMSLELEKAGIAVDKQHHEVATAGQAEIDIRFA
                     SLKRMGDNLQYYKYILRNVAKKHNKTVTFMPKPLYADNGSGMHTHISLWKDDKPLFAG
                     NGYAGLSDNALFFIGGILRHAPALTCLTNPTTNSFKRLVPGFEAPVNLAYSARNRSAA
                     IRIPYVANPKGRRIEVRFPDPTANPYLAFAAMLMAGLDGVQNKIHPGDPMDKNLYDLE
                     PEEAAKVPHPCASLDEALDQLDKDREFLTKGGVFTDDMIDAYIGLKMEEVT"
     gene            complement(1481..1819)
                     /locus_tag="VFO30_01820"
     CDS             complement(1481..1819)
                     /locus_tag="VFO30_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9418053.1"
                     /translation="MKKIEAIIKPFKLEEVKEALAELGIEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKVEVVMADDMVDKAVSAVVNAAKTGKIGDGKVFVLAVEHAVRI
                     RTEEIGERAV"
     gene            2018..>2928
                     /locus_tag="VFO30_01825"
     CDS             2018..>2928
                     /locus_tag="VFO30_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9418054.1"
                     /translation="MSRDSFVHLHLHTEYSLLDGAVRMRELMKEAVEMKMPAVAVTDH
                     GNLFGAVEFYQEAKHAGIKPIIGCEAYVAPGSHKDRPASMRDAAYHFTLLARDEMGYR
                     NLVKLVSIAHLDGFHYRPRIDKELLARHSAGLIGLSGCLASEVSSAIQANKIDIATKT
                     AAEYRDILGAENFFLEMHDHGIEEQKYCNLVVPNIARDLGIGIVAANDVHFLRPTDHD
                     AHDVMLCIGTGKMVQDENRMRYAPELYFKSPQEMSALFKDFPEAITNALEIAERCNLE
                     IELGKSKYPEYPLPEGVMREQYLRELCA"
BASE COUNT          590 a          817 c          849 g          672 t
ORIGIN      
        1 gcgtgacctc ttccatcttg aggccgatgt acgcgtcgat catgtcgtcc gtgaagaccc
       61 cgcccttcgt caggaattcg cgatccttgt cgagttgatc gagcgcctcg tcgagcgacg
      121 cgcaggggtg cggcaccttc gcggcctctt cgggctcgag gtcgtagagg ttcttgtcca
      181 tcggatcgcc cgggtggatc ttgttctgca cgccgtcgag gccggccatc agcatcgccg
      241 cgaatgcgag atacgggtta gccgtcgggt ccgggaagcg cacttcgatg cgccgcccct
      301 tcggattggc cacgtacgga atgcggatcg cggccgagcg attgcgcgcg gagtacgcga
      361 gattgactgg cgcttcgaaa ccagggacga gacgtttgaa gctgttcgta gtcggattcg
      421 tcaggcaagt gagcgccggc gcgtggcgca aaattccgcc gatgaaaaac agcgcattat
      481 cgctcaaacc ggcataaccg ttgccggcga agagcggctt gtcgtctttc caaagcgaga
      541 tgtgcgtgtg catgcccgac ccgttgtcgg cgtaaagcgg tttcggcata aaggtgacgg
      601 tcttattgtg cttcttggcg acgttgcgca ggatgtattt gtagtactgc aagttgtcgc
      661 ccatccgctt cagggaggcg aaccggatat cgatctcagc ctggcctgcg gtggcgactt
      721 catggtgctg tttgtcgacg gcgatgcccg ctttctccag ttcaagtgac atctcgttgc
      781 ggatgtcctg caatgaatcc atgggtgaga cggggaaata gccttccttc ggccgaatct
      841 tgtagcccag atttggaaac tcctcgcgcg cgctgttcca gtggccctcc atgctgtcga
      901 cgcggtgaaa cgacgcgttg ccgctgtagc tgaaccgcac gtcgtcaaag atgaaaaact
      961 ccgcttccgg tccgaagtaa gccgtgtcgg cgatgccggt gctttgcaga taagcttcgg
     1021 ctttttccgc gacgccgcgc gggtcgcgat cgtacggctc gcgtgtgatt ggatcgacaa
     1081 ttgtgcaaat gagcgaaagc gtcggctcgg cgttgaagat gtcgatccac gctgtggtcg
     1141 cgtcgggaat gacgagcatg tctgaggcgt tgatgacgcg ccacccgcga atgctcgagc
     1201 cgtcgaagcc aagcccgtcg gtaaaaatgc cttcgtcgta ctctgtgagc gtggtggtga
     1261 aatgctgcca cgtaccgagg aggtcggtga acttgaagtc gatcaacttc acctcgttgt
     1321 ctttgatcat tctgctgaca tcagatgcgg ttttgtcttt ggccatggga ttggttggat
     1381 ggttaaatcg ttgaatggtt gaatcgtgaa atcattaatt ctcgaaccgt gttaacgttg
     1441 taacgcttca acggttcaac gcgaggcgcg ccaggcgcgc tcacaccgct cgctcgccga
     1501 tctcttccgt gcggatgcga accgcgtgtt ctactgcgag cacaaaaact ttgccgtcgc
     1561 cgatttttcc cgtcttcgcg gcattcacta cggcgctgac cgctttgtcg accatgtcgt
     1621 ccgccatcac gacttccact ttcaccttcg gcaaaaaatc gacggtgtat tcgctaccgc
     1681 ggtaaatctc agtgtgccct ttttggcggc caaaaccctt cacttccgtc acagtcatgc
     1741 cttcgatccc gagctcggcc agcgcttcct tgacctcctc gagcttgaac ggcttgatga
     1801 tggcttcgat ctttttcatc tcgatctaac ctgtcggtcg ctccgcgttt tgcaattgca
     1861 aacgccagtc cggatcggga cagctgagcg gcgcacgcgc tctcttaaca accggacagg
     1921 cgcgatgcaa acaggatttt ctgcccaaaa cgcaatcgtg agctcccatc cctcgtaaat
     1981 ttgacgttcg aagattctcc gctttgtaat cgtcgagatg tcccgcgatt cattcgttca
     2041 cctccatctg cacaccgagt actccctcct cgacggcgcc gtccggatgc gggagctgat
     2101 gaaagaggcg gtggaaatga aaatgccggc ggtggcggtg accgaccatg ggaatctttt
     2161 cggtgcagtt gagttttacc aggaagcgaa gcacgcgggg atcaaaccga ttatcgggtg
     2221 cgaggcgtac gtcgctcccg gctcacacaa agatcggccc gcatcgatgc gggatgcggc
     2281 atatcacttc accctactgg cgcgcgatga aatgggttac cgcaacctgg taaagctcgt
     2341 ttcgatcgcg catctcgacg ggtttcacta ccggcctcgg attgataagg aacttctggc
     2401 gcgacattcc gccggtctga ttggcttgag tggttgcctg gccagtgaag tgagctccgc
     2461 aatccaggcg aacaagatcg acattgcaac gaaaacagcg gccgagtacc gcgatatcct
     2521 cggggcggag aatttcttcc tcgagatgca cgaccacgga attgaagagc aaaaatactg
     2581 caacctcgtg gtgccgaaca ttgccaggga cttgggcatt ggaattgtcg cggcgaacga
     2641 tgtgcatttc ctgcgaccca ctgatcatga cgcgcacgat gtcatgctct gcatcggcac
     2701 aggcaagatg gtacaggatg aaaaccgaat gcgctacgcg ccggagttgt atttcaaatc
     2761 gccgcaggaa atgtccgcgc tgttcaaaga ttttccggaa gcgatcacaa acgcgctcga
     2821 aatcgcggag cgctgtaacc tcgagattga gctcggcaaa tcgaagtatc ccgagtatcc
     2881 gctgccggaa ggtgtcatgc gcgagcaata cttgcgcgaa ttgtgcgc
//
LOCUS       DASTDG010000062         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_613640, whole genome shotgun sequence.
ACCESSION   DASTDG010000062 DASTDG010000000
VERSION     DASTDG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613640"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFO30_01830"
     CDS             <1..471
                     /locus_tag="VFO30_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418055.1"
                     /translation="HPGVCAGYRLFTREGSIAFLPDNEPFEPLKLKLAARDGIPPEKA
                     RAQAAVERSKLVEFLKDADVLIMDAQYTDEKYLEHIGWGHGALSRVVSLALEARVGKL
                     FLFHHDPEHDDHKIDEILEAARLLVVESGRPLQVEAAREGAEVWLSAKSAQLHR"
     gene            507..580
                     /locus_tag="VFO30_01835"
     tRNA            507..580
                     /locus_tag="VFO30_01835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Gln,seq:ctg)
     gene            complement(615..1124)
                     /gene="tadA"
                     /locus_tag="VFO30_01840"
     CDS             complement(615..1124)
                     /gene="tadA"
                     /locus_tag="VFO30_01840"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET9418056.1"
                     /translation="MGPNCAGRVAEPLMQTDVDLMREALRLARKAYDAEEVPVGAIVT
                     RDGKIIGRAYNQVELLKDATAHAEMLALTQAEAAMGDWRLSDCDLYVTKEPCAMCAGA
                     IVHVRIRRVIFGCPDERVGAAGSVINLLQMPRFNHQCAITGGVLQTECAAILQKFFRQ
                     KRAVNPGQP"
     gene            1142..1786
                     /locus_tag="VFO30_01845"
     CDS             1142..1786
                     /locus_tag="VFO30_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET9418057.1"
                     /translation="MKNVVLSLLLLPLWLQTAAAASEQEIVDRSARMVREFRSMPEQQ
                     IPRAVLQRARGLAVLTVVKAGFIFSGKAGHGVVVARLDHNRWSGPSFIGTGGAGWGLQ
                     IGAEVTDFVFVLNNRAAVRAFSRDGNVTIGADVSAAAGPVGRDLQAGVAPTAAVYTYS
                     RSKGLFAGVSLEGAVIVTRAAANSRYYRRPVTAADILSGRVSPPPGANRLRAAL"
     gene            1833..2345
                     /locus_tag="VFO30_01850"
     CDS             1833..2345
                     /locus_tag="VFO30_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418058.1"
                     /translation="MRCFYILICAFVALSSGRAQEQERKLVDRLLNPDMSLANTAQNK
                     KFTTDSKVADKPANVGTFYLEQKPAPKSYSATREFTAARFHSPVFVAGQKTANTSSRT
                     EIQNADTRPAAPRARRVQETHDARKVATSRNYAGERPFLGRGKSQKSLDRKNPPMTID
                     DVRELLNKNK"
     gene            2384..3553
                     /gene="tyrS"
                     /locus_tag="VFO30_01855"
     CDS             2384..3553
                     /gene="tyrS"
                     /locus_tag="VFO30_01855"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9418059.1"
                     /translation="MKADEALAILKQGAAQIISDAELREKLALGRPLRVKLGVDPTTS
                     DIHLGHTVVLRKLRQFQDLGHQAVLIIGDFTGMIGDPSGRSATRPQLLHDMVMANADT
                     YREQAFKILDEKRTEIVFNGDWFRKMSFEDVIRLNSRVTLQQMLQREDFHERIENEQP
                     IHAHEIQYPIMQGWDSVMVKADVELGGTDQLFNILIGRDFQRDEGLPQQIVFLLPILE
                     GLDGARKMSKSFGNFVAVNEPAGEMFGKLMSISDELMARYYPILLGRELARDAHPMEA
                     KKQLAFEIVQTYHSAATAKAVRDEWTTRFSERRLDDAELPAFSAASGDAAVAVIVTAY
                     AQAFGLTKSRAEASRLIKQGSVQLDGEKIMDPKNALPLRRGQILRLDKTHAVRIG"
BASE COUNT          785 a         1054 c         1141 g          733 t
ORIGIN      
        1 caccccggcg tctgcgctgg ctatcgcctt ttcacgagag aaggttcgat cgcgtttttg
       61 ccggacaatg aacctttcga gccgttgaag ctgaaattgg cggcgcgaga tggcataccc
      121 cccgagaagg cccgcgcgca ggcggcggtc gaacgctcga agctggtgga atttctcaaa
      181 gacgcggacg tgttgatcat ggacgcgcag tacacggacg aaaaatatct cgagcacatc
      241 ggctggggcc acggcgccct gagccgcgtc gtctcgctcg ccctcgaagc gcgggtgggc
      301 aaactttttt tgttccacca cgatccagaa catgacgacc acaaaattga cgagatcctg
      361 gaggcggcgc ggctattggt agtggaaagc ggccggccgc tgcaagtgga agcagcccgc
      421 gagggtgccg aagtatggct gagtgcgaaa tcagcgcagt tgcaccgtta gcggtgcgat
      481 ttagatttcg cggctcgaac gacttttgcc tcgtggtgta atggtagcac tagagactct
      541 ggatctctct gtctaggttc gagtcctagc gaggcagcca ttgcacaatt gccgattttc
      601 gatcttcagg cgactcacgg ctgaccagga ttcactgcgc gcttttggcg gaagaacttc
      661 tggaggatgg cggcgcactc agtttgcagg acgccaccgg taatggcgca ctgatggttg
      721 aaacgcggca tttggagcaa gttgatcaca cttccggcgg cgccaacgcg ttcgtccggg
      781 cagccgaaga tcacgcggcg aatgcgcacg tgcacaatcg cgccggcgca catcgcgcat
      841 ggttctttgg tcacatagag atcgcagtcg ctgagccgcc aatcgcccat ggccgcttcg
      901 gcttgcgtga gcgcgagcat ctcggcatgc gcggtggcgt ccttgagcag ctcgacctga
      961 ttgtaggcgc gcccgattat tttgccgtcg cgtgtgacga tggcgccgac gggcacctcc
     1021 tcggcatcat aggctttgcg tgccaaccgc agcgcttcgc gcataagatc gacatcggtt
     1081 tgcatgagcg gctccgcgac acgtccggca cagtttggcc ccacgaacta cttatgtcgc
     1141 gatgaaaaac gtcgtcttaa gcctgttgct tttgccactg tggctgcaaa cggctgccgc
     1201 tgcgagcgag caggaaattg tcgatcgctc tgcgcggatg gttcgcgaat tccgctccat
     1261 gcctgaacaa cagatcccgc gcgcggtgct gcagcgggcg cgtggcctgg ccgtgctgac
     1321 cgtcgttaag gccgggttca tcttcagcgg caaggccgga cacggggtag tcgtcgcacg
     1381 actcgatcac aaccgctggt ccgggccttc gttcatcggt accggcgggg ccgggtgggg
     1441 attacagatc ggcgcagaag tgaccgattt cgtcttcgtg ttgaacaatc gggcggccgt
     1501 gcgggcattc tcgcgcgatg ggaatgtcac aatcggcgcg gacgtgagcg cggcagcggg
     1561 cccggttgga cgcgatttgc aagctggcgt cgcgccaact gccgccgtct acacatacag
     1621 ccggagcaaa ggtttgtttg ctggcgtctc tctcgaaggc gcggtcatcg tgacgcgcgc
     1681 ggcggcgaat tcgcgctact atcgacggcc agtcacggca gcggatatct taagcggccg
     1741 cgttagtccg ccgcctggcg caaaccgatt gcgcgcagcg ctgtagcgca atttccttta
     1801 caaacgcgcc gcaaagttgg caccgtggta atgtgcggtg tttttacatt ctaatctgcg
     1861 cgtttgttgc gttgtcgagt ggtcgcgctc aggagcagga acgcaagctc gtcgatcgcc
     1921 tgctcaaccc ggatatgagt ctggccaaca cagcacagaa caaaaagttc acgaccgaca
     1981 gcaaagtcgc cgacaagcca gcgaatgtcg gcacgtttta tctcgagcaa aaacccgcgc
     2041 ccaagagtta ctccgccacg cgcgagttca ccgcggcccg atttcattcg cccgttttcg
     2101 tcgccggtca gaaaaccgcg aacacttcct cgcgcaccga aattcagaac gccgatacgc
     2161 gtcctgcggc accacgcgcc cgccgtgttc aggagacgca tgacgcacgc aaagttgcca
     2221 cgagtcgtaa ttacgccgga gagcggccat ttctcggtcg cgggaaaagc cagaaatcgt
     2281 tagatcgaaa aaatccgccg atgacgatcg acgacgtgcg cgagttgctg aacaagaaca
     2341 agtgactttg ccgccgggga cggcggcctc tacaatttgc gccatgaaag cggatgaggc
     2401 gcttgcaatc ctaaaacagg gcgccgcgca aatcatcagc gatgccgaac tgcgcgagaa
     2461 actcgcgctc ggccgcccat tgcgtgtcaa gctcggggtc gatccaacga catctgatat
     2521 tcacctcgga cacaccgtcg tattgcggaa gctccgacaa ttccaggacc ttgggcatca
     2581 ggccgtgctc atcatcggcg atttcacggg aatgatcggc gacccgagcg ggcgttcggc
     2641 gacgcggccg cagctgttac acgacatggt aatggcgaat gccgacacgt accgcgagca
     2701 agcgttcaaa atcttggacg agaagaggac cgagattgtt ttcaatggcg actggtttcg
     2761 caaaatgagt tttgaagacg tgatccggtt gaacagccgc gtcacgctcc agcagatgct
     2821 gcaacgcgag gatttccacg agcgaatcga gaacgagcaa ccgatccacg cgcatgaaat
     2881 tcagtatccg atcatgcagg gttgggactc ggtgatggtg aaggccgatg tcgagctcgg
     2941 tgggaccgat cagcttttca atattttgat cgggcgcgac ttccaacggg acgaagggct
     3001 gccacagcag atcgttttcc ttttgccaat ccttgaggga ctcgatggcg cgagaaagat
     3061 gagcaagagc ttcggcaact tcgtcgctgt caacgagccg gcgggcgaga tgttcgggaa
     3121 gctaatgagc atttctgatg aactaatggc gcgctactat ccgattttac tcggtcgcga
     3181 gctggcacgc gatgcacatc cgatggaggc gaagaagcag ctcgcgtttg agatcgtgca
     3241 gacttaccac tcggcggcga cggcaaaggc agttcgggat gaatggacta ctcgcttcag
     3301 cgaaagacgc ttggatgacg ccgagctgcc cgcgttcagc gccgcgtccg gcgatgcagc
     3361 ggtggcggtt attgtcacgg cctacgcgca ggcgtttggc ctaacgaaat cgcgcgccga
     3421 agccagtcgc ttgatcaaac agggaagcgt ccagctcgac ggagagaaaa ttatggaccc
     3481 gaaaaacgcg cttccgctgc gccgcgggca gatccttcgg ctggataaaa cgcacgcggt
     3541 gcggatcggc tgaagtacag caatagagca cgaagtgttt tcaacttcgc gctctttgcg
     3601 ggaatgattg tcgatcttta ggcagccgca gcttcgaaca tcggcagcac cggcacgtgt
     3661 tcgagatagg gcgtcgcgat ggcttcgaaa tgtgaacgaa tgctgcgcaa ctc
//
LOCUS       DASTDG010000063        22844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1032157, whole genome shotgun sequence.
ACCESSION   DASTDG010000063 DASTDG010000000
VERSION     DASTDG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 22844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22844
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032157"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFO30_01860"
     CDS             complement(<1..1082)
                     /locus_tag="VFO30_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9418060.1"
                     /translation="MSAVERTLHFVGRPLVRVAYRIATFGFDSLPAGGFLLLPNHITW
                     VDAIVLQAACPRPIRYVIDQEFYHKPMLHPFLRVLGCIPMDIRHPRSAIRAATERIAA
                     GEIVCLFPEGQLERRGVLLRLQRGYELIARHARTRVVPVWLDQLWGSIFSFKGGRFFT
                     KLPRRIPYPVTVAFGKALDAEKANIATVREELLKLGEFCYSRRSMFDRHLARDCIRGL
                     KRRSFATAVTDGIDGSRLSRAKLLGAAAALSRFLRREFPDQRLAIVLPAGRGAVVANL
                     ATALADKVAVDLNFTSGRAANEACCRRAELRVAISASPFIDRIKDFPWPERIVKLDEL
                     MPRLKRQVLFWWMMSILCPAWLLARLL"
     gene            1063..2442
                     /locus_tag="VFO30_01865"
     CDS             1063..2442
                     /locus_tag="VFO30_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9418061.1"
                     /translation="MRSTADMLGMFVIYNRGGAHCNRQLSMENRSGTTRTFARSAVWL
                     LLGINLFNYIDRQILAAVLPRIRHDFLAGDPNQNSKAGLLTTAFLFSYMFTAPVFGWL
                     ADRFSRWVLVGISVALWSVASGWSGLATSFGILLWTRAFVGIGEAGYGPAAPTIISDL
                     YPLERRGRVLAYFYLALPVGGALGYAFGGMIADLLGWRWAFYLVVPPGLLLAAFCFFM
                     RDPRADAQRGFRLGARPRWQDYVRLLRIPSYVLNTAAMTALTFAIGGFSAWLPDYIYT
                     DRRAEFARDPNLLGKIDLIFGAVVAVAGLFATLFGGWAGDWLRNKFKSAYFLVSAVGV
                     LLAFPATIAMLYVRFPGAWVCIFVAVFFLFFNTGPSNTALANVTSPAVRATAFALNIL
                     IIHALGDAISPPLIGAIRDRWNMNAGFLAVSGTMLLAGVFWLVGARFLPRDTMKVERA
                     TEQSAARAA"
     gene            complement(2530..3510)
                     /locus_tag="VFO30_01870"
     CDS             complement(2530..3510)
                     /locus_tag="VFO30_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9418062.1"
                     /translation="MKTSFCLGALLAIVGLAIAPLVSKAQTTSTTSTSTTYVQTSKII
                     GSKVKTAQGEEIGEIRDVVLDRNSGCMAYTVLSTGGTTARVAGQAKTVAVPWTVYSVT
                     SDPTVYTVRIDRERIYSAPVFDYARIDEYSRPDYINNVYSYYGVSAPAGVSTSVTGTT
                     TGTTTTATGAATGAAGAYGSPSPGAAGATGTAGAASPYASASPRMTPAVGASASPSAT
                     ASAAATASPRGRHGARRGASPGASPSERMSPGAAESPEARATESSMTGREATESTGRG
                     RTGTESATGAASGMETGASPAGEHKKGGRKHAHEGAAEPSATPKTSQEQE"
     gene            complement(3580..5370)
                     /locus_tag="VFO30_01875"
     CDS             complement(3580..5370)
                     /locus_tag="VFO30_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418063.1"
                     /translation="MKKTFAPLLIIVVVAAIYAPTLGNGFVWDDTALVLRDPLIRSWR
                     LIPEGFNHFLFADATPSDFYRPLQRVTYTLEYVAVGFRPMLYHLSSIISHAAAVVALF
                     IFAHELLLSYGVDDRKRRLIALLATLVWAIHPVQIPAVAYVSGRADPLAALFGFTGLY
                     LVIRSTRATGASRWRFFIGSGLAFLLSAFCKEAGLIFPVLALMLFAVRKAWIDMWKTV
                     AVAAFIAAIYFSLRLGAEHYHPPAMSAPAPPLVRPIIAARAVAEYAGLMLFPLNLHAD
                     RDVETHPSGWNDASLRGAAWRELQTLLGIVLIAALLYWTWRARKRNPAAFALLLIALI
                     SYLPISGIVALNATVAEHWLYLPSAFLFLAAAVELGSLRCARSTVTVAAGVFTLWLLF
                     LGARTFVRTFDWKDQRTFFARTIAQGGDSVRMLVNLGSLEASEGRLQEAALHLRAALQ
                     KTPDQPLAVINLAAVLLQQNDLKLARQLLERATQMPLVNAQAHELLAVLEHKENGGVD
                     VLRMRLAARTGPPDWSIEKRYIQLLDQTGATPAAINELLTCLQTQWYRAESWQLLGQL
                     HAKLGHSTQANRALAQARDYDAHLGSPSVL"
     gene            5424..6191
                     /locus_tag="VFO30_01880"
     CDS             5424..6191
                     /locus_tag="VFO30_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9418064.1"
                     /translation="MSILRRAAVLVLLSLTSNACAAGVPEDCTQLIVGLAPDWNSMRG
                     TLQRFERTRGGEWRAVSKAVPVLFGKNGLAWGIGLAGQHEAGLHKKERDGRAPAGIFE
                     IGQVFGYDSKLPPGGDYPYHQVTEADIWSDDPRSPNYNRHVVIDPQNPPDNYSHEKMR
                     SGDFAYRWLVDVRHNSNLPIPGAGSAIFFHIRRGVNRPTTGCTTMAEPELVKLITWLR
                     ANQHPCYALLPVAEYERKWQDWHLPPSKKVRTAVNSR"
     gene            6228..6530
                     /locus_tag="VFO30_01885"
     CDS             6228..6530
                     /locus_tag="VFO30_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418065.1"
                     /translation="MPRRANPDSEANIRRIDTRMRAKKQTHGFQVHFLRGATAVTKMF
                     SDTLYGGKEAARRAARKFKEIAMRGLPQRNAAGSKSTTRRKTTTRARRRSATMRRR"
     gene            complement(6511..7920)
                     /gene="rsmA"
                     /locus_tag="VFO30_01890"
     CDS             complement(6511..7920)
                     /gene="rsmA"
                     /locus_tag="VFO30_01890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9418066.1"
                     /translation="MNSLAIFAVRSEPIVDLTMKLSDITATLQEIRVSPVKKLGQSFL
                     HDQNLARCIVKAAELTPEDYVVEIGPGLGALTSIILDTGARVLAIEKDTRLAAFLREH
                     LAHPRLEVLNEDALDFDLRALFCERRVKLLGNLPYTISSPLLAKYVQYPSPISLWLFT
                     LQREVAARLTASPATREYGALTVRLQLHHRVEHLRSIPATVFVPEPEVASTLVRIVPR
                     DPLELPACDDELLNDLLRRGFTHRRKQLHKALSDFTADWPKIAAKLEIEPKARAENLS
                     LEQWIALANQIAPIHSSEQQVFWQERFPVVDEMDRILRYAYRSEVHGDKSRHRAVHIL
                     IFNEAGEVYLQKRARWKDRHPLQWDSSAGGHVSAAEGYDEAARRELREELRIDVPLEK
                     LLKLPASTRTDQEFIWLYRGHVQNAVRPNRSEIEAGGFFRPEVVDGWIAARREDFTPA
                     CMECWHAFRQHGVLSAAAL"
     gene            8079..10067
                     /gene="tkt"
                     /locus_tag="VFO30_01895"
     CDS             8079..10067
                     /gene="tkt"
                     /locus_tag="VFO30_01895"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9418067.1"
                     /translation="MSLNVEILSRAANEARGLCMDAVQASQSGHLGLPLGCAEMGAVL
                     YGYALKHNPDKPRWIGRDYFVLSAGHGSMFLYAWLHLSGYDLPMSEIKRFRQLHSKTP
                     GHPEFFETPGVECTTGPLGQGVGNAVGIAVAMKMAAARFNSDEHRIFDQHVICLAGDG
                     CMQEGVASEASAFAGHFGLDNLILIYDSNAVTLDAPAKETQSEDTGKRFAAYGFDVQE
                     IQGDDMQQFLDALNIAKERDNGKPQFIIAHTLIGKGIPEVAGTYKAHGEAGAKFVDAG
                     RKGLGLPDEHYYVSKEVYEYFAEHKKKLLADYDRWEKNYDEWRKKNPERAKILDDGIE
                     RRVPADLFSKIPEFPKDAKLATRKAGGEVLQPIAREMPLLIGGSADLYGSTMNYIKDG
                     GDFTRDTSAGRNIRFGIREHGMCAILNGISYHGIFRASGATFLVFTDYCRASIRLGAL
                     SKLPNTYIFTHDSIGVGEDGPTHEPVETVSSVRVMPQIDLIRPADPEETAGAFVAAME
                     RNNGPTMIALTRQTVPILNDVDVAVRRDGVLRGGYIAKKETGKLDLIIMSCGSELQHA
                     LAAAKQLGDNVRVVSMPCFERFERQPQGYRDEVLPNECRRRIAIEAGVPDTWFRYVGL
                     DGKVIGLHRFGLSAPGAEVMKEFGIDADHVIQAAKSLW"
     gene            complement(10152..11645)
                     /gene="dnaB"
                     /locus_tag="VFO30_01900"
     CDS             complement(10152..11645)
                     /gene="dnaB"
                     /locus_tag="VFO30_01900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9418068.1"
                     /translation="MATQPAAWTGETPRKQSGKKPGRGWPGGNGGSDGGKSLTASSQD
                     IHRTPPHSVEAEQGVLGSMLISPRDAIAECVEKINENYFYVPAHQTIYNVLVDLWNTG
                     QAIDLITFTQVLRDRNLLDAVGGPAFVTSLFTFVPTAANVGYYLDIVRDKFVLREIIS
                     AATESVRRAYEEQDEVSNLLDEVEQKIFAVGEDRFKGHMLSMKDQVMEAIEAIEKLYE
                     RKGGITGVSTGFVEFDRMTSGMHAAEMIVIAGRPSMGKTALAMNIAEHVAINEKLPVG
                     VFSLEMSGQQLVQRLLCSRARVNLQKVRDGFLAERDFPSLTAAAAKLAEAKIFIDDSA
                     ALDILALRAKARRLKAQQDVQLLVIDYLQLLRSTSRRAQDNRQLEISEISAGLKGLAK
                     ELKIPVIVVAQLNRQPEQRSGGKPRLSDLRESGAIEQDADLVGLLVRPEIYEEDEEAR
                     AEKAGEAELIIAKQRNGPVGEIALTFLKEFTRFEDRARNVTEPEEAF"
     gene            complement(11646..12200)
                     /gene="rplI"
                     /locus_tag="VFO30_01905"
     CDS             complement(11646..12200)
                     /gene="rplI"
                     /locus_tag="VFO30_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9418069.1"
                     /translation="MPSTEVILTENVPGLGAEADLVKVRRGYARNYLLPNGKAYEVTP
                     AALRQLDNLKKKRAEREARELNEAEDIARRIGKTRVTFTLATGETGKAFGSVTAQDIV
                     NRLRNEIGHEIDRHKIVLERPIKDTGEHEVAIKLHHDVTAHFKFDVKSAEEPKPQQTA
                     AEEPEKKHRSFFRRKKEERKEPGK"
     gene            complement(12347..12901)
                     /gene="sufT"
                     /locus_tag="VFO30_01910"
     CDS             complement(12347..12901)
                     /gene="sufT"
                     /locus_tag="VFO30_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HET9418070.1"
                     /translation="MYENTEFSLSRDVEAIEIPSGRKFTLENGTQGVITQTLGGSYTI
                     ATPYGLSRIAEKDVDALGLEKPQARAETTPASRTNGEVSEEDVWNQLRQCYDPEIPVN
                     IVDLGLVYDCRLVKKDDGGTRVEVKMTLTAPGCGMGPAIAHDAQAKILSIDGVDEADV
                     QLVWDPPWNQNMISQAGRMKLGMI"
     gene            complement(12999..14222)
                     /locus_tag="VFO30_01915"
     CDS             complement(12999..14222)
                     /locus_tag="VFO30_01915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9418071.1"
                     /translation="MPQVPIIMPQLGESIAEATIVDIKVKPGDEVAADQEIIDVETNK
                     AVMGVTTPCKGKIDNIAAQLKETYPIGAVLGYVEASEADAARFTKQAPAPPQRTVAEE
                     IPARADQEVKVAQPSRLPVGDGNRDGRPTLPVPATLKGASFISPRVRARMAELQLTNA
                     DLAGITGTGAGNRVTIKDLENFLHKIDSETAQEASAIRAGVADAMRRSWSRPLSTVGS
                     SVNLDPVLEDRQSREPKPGPGLYALRALALALAENPKAAAKLIGNRVIQSNSIDVGIA
                     VEAEDGIMVPVIRSADKTSLVELTAKYQELVELARKRRISPDMQAGGIATVSNFGIFG
                     VEWGTPIPLPDQTLLLGLGAGKKLPSWDESKKEFVPRTEAQITLSFDHRSIDGGGGAR
                     LLKRVIELLENPKQL"
     gene            complement(14228..15196)
                     /locus_tag="VFO30_01920"
     CDS             complement(14228..15196)
                     /locus_tag="VFO30_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9418072.1"
                     /translation="MSITYLEAIREAQAKLLRDDERVFIYGQDVGGTFGGAFKATKGL
                     AQEFPGRVLNTPISEDAMVGTAIGAAMHGMRPIVEMQFADFSSTALNQILNNAGTQYW
                     RTNVPVPITIRLPSGGTKGSGPFHSQSMESIYAHYPGLIVMTPATVEDAYTMLIDAVG
                     IDDPVVYCEHKYLYYHLKADKLPTEGLPIGKARIAREGHDLTIVAYSVMVQEALTAAD
                     ELAREGLQIEVVDLRTVKPLDTDTVLASVARTARLLCVGESFPWGGATAEVIARVTSE
                     GFHLLDAAPLRINAKDTPIPYHPNLWSAHRPNAQKIVAKARQLLRE"
     gene            complement(15247..16287)
                     /locus_tag="VFO30_01925"
     CDS             complement(15247..16287)
                     /locus_tag="VFO30_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9418073.1"
                     /translation="MLQTAARSKKSALKKAAPHERFLEAFRWMLLARTLEEKLVSLYR
                     GGQITGGVYIGKGQEAVSVACGLFLQKGDIFAPLIRDQAGRSAFGEPLVDVARTYLGS
                     RHGPMRGRDGNIHRGKPREGQLAMISHLGAMVSVTVGALMAKRFRGEKNFIGLTSIGE
                     GGMQTGSFHEGMNLAAVEQVPLVVVATNNHYAYSTPNEREFACQELVDRAKGYGFEGY
                     KLDGTDLTQCLEVIESAVKRARAGRPPQLVVASTLRLSGHGEHDDASYVTDEIKRQPF
                     AQDCLKLTEKKIVDLELLDPATLQSWREDAAAQVDEAIATAQKEPAPDGAEEDWCAIS
                     TRALADHAESVE"
     gene            complement(16436..20221)
                     /gene="smc"
                     /locus_tag="VFO30_01930"
     CDS             complement(16436..20221)
                     /gene="smc"
                     /locus_tag="VFO30_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9418074.1"
                     /translation="MHLQSLELFGFKSFADKTVFSFHQGVTAIVGPNGCGKSNLLDAV
                     RWVLGEQSAKSLRGGEMADVIFNGTDTRKPLGFAEVSVTFGNCEAELGVEWNEVRVTR
                     RVYRDGNSEYLLNKTACRLRDIQNLFADTGVARAAYSMMEQGRIDMILSSHPEDRRAV
                     FEEAAGITKYKAQKREALRKLEATEANLLRIGDVIKEVKRQIGSLQRQAGKARRYQAL
                     HADLRVLDLHYSRKQLAALEAGLAQCRAEIDRIVESEQQTRAKIDAGETGQAEERHAL
                     DKIDIEIADARAEAQRVQSDITARHSRIEFNRLRAAELAELIERARNDIAAAEAKQRE
                     LASQAEQLDGAVANTRGILQAKETELSAATEFLASIRAQREARQSQRTDVRESVAKLE
                     QRIASLQDDLSSLNARREVTSQQLAHLAEEISRTIALRERLGAEITAARSQTDQEQQT
                     LARRSAETRAAEKSLQRERELLSRAESDWASIDRALAEKRSALEVLRQLNQQGEGLEQ
                     GSQAVLRGMDDPERFRDTVVGPLVAQLHVERSFVPAIEGALGRNLHAVVLKDGGSVAE
                     IVARLTKKKFGQAALCVPRLGGSAPESVRKTLPPGAIGWAVDKVTAPRAFEPLIRRLL
                     SEVVIFADLERALACKKQEPALAMATLDGEFISAEGVIFGGSNVARRDSWLDRKARVT
                     ALADEENALAGQRELLLERCEELRTELDKASHELERTRAAEQAAHVVNSTAAGRISLL
                     ETEQETVTGKIDNLQSDTRTLEEQIKSADERVAQIETDLSELRRELDRRQNDRASVEQ
                     DENEARLQEERAREQITELKLALATEQQRYENLRAQCEAIRARDLELTDLIAARRADI
                     VGYENKMAAQADESHQAERLIAEQTTRAMELETYGAALGQRRATRTAEVERRETELRE
                     LRHSLSNLQNDRAQQQVRESQLRMEIQHLGDRVGRQYHFDLRAAAPDEAVFEKTLRAQ
                     FKRWIDRSAGDANTGGEAASDLASRSESAIHLASDLSDEDLQKLIVDLTQQLDNMGPV
                     NLEAVQEYDELEERYKFLEAQNTDLTNSRRELLEVIARINATTKQMFAETFTQIRGNF
                     REMFAEMFGGGRADLSLLDESDPLNCGIEITAKPPGKQLQSISLLSGGERSMVAIALL
                     FAIYMVRPSPFCVLDEIDAPLDETNINRFLAILERFVSQSQFIIITHNKRTIAKADVL
                     YGVTMEERGVSKLVTMKLIDAPADKSQPETGETVSSQRQFALAENGRSETESSFAAR"
     gene            20388..21755
                     /locus_tag="VFO30_01935"
     CDS             20388..21755
                     /locus_tag="VFO30_01935"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET9418075.1"
                     /translation="MLAHAKLQDMRSDQLEDYYTFLRFPSISTDSDYAAKVSECAHWL
                     VEKLSSVGLEAKLMPTPGHPVVWARNEHRPDRRTVLIYGHYDVQPPDPLELWESPPFE
                     PVLKDGYVFARGATDNKGQILSHVLGIEEALERDKDLPVNVDLVIEGEEEIGSANLGN
                     FLSQNREALECDVVVVSDTGMIAPRTPTMTYALRGVAAVELKVTGPKIDLHSGIYGGA
                     VANPLAAMSRLLASLHDRDGRVAIAGFYDDVEPLQDWEREMWSKLPIDADAMVLGESG
                     ARELFGEKGFTTLERIWARPTAEINGMGGGYQGKGTKTVLPSYAFAKLTFRLVPNQNG
                     ERIVDLARKHFENNLPPGVTLEVTSGHAGPWYRTDPHNRFGQAAQRALRKTFDKEPVL
                     IREGGSIPIVSQFRDILGVETLLLGLALHDCRAHSPNENFPLENLDAGIRMNQAVLRE
                     LAAIG"
     gene            21767..>22844
                     /locus_tag="VFO30_01940"
     CDS             21767..>22844
                     /locus_tag="VFO30_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9418076.1"
                     /translation="MKLARRWFVAMALPLVLTVSAPECGAAQDDSVIDKIKHLFVKPT
                     PTPTPKPRKKRTKKKSSPTPSPTPSASAAAAATASPAPSESAPIVPAATASPAASATA
                     TATPAAAATVPPPSPSPTGSPLPRHGRSQHLEPVRPIAPGPHAKFRTSVSPPPEASPP
                     AIRTPLPLTSPVSAATASPLPAATASRAATPAAKSTHAPNATISPDEISGYESYPAEV
                     RHVLDSALALTTQNLGYKYGSADPANGGMDCSGFIYYVVSQCGIKDAPRDAREQYIWV
                     RKAGNFQAVLAHRDDTFELDALKPGDLLFWAGTYNVSRDPAITHTMIYLGREKGTNQR
                     VMVGASDGRTYRGQQRFGVSVFDFK"
BASE COUNT         4406 a         6824 c         6731 g         4883 t
ORIGIN      
        1 aggagccgcg cgagcagcca cgccggacac aagatcgaca tcatccacca gaacaggact
       61 tgtcgtttca ggcgcggcat cagctcatcg agttttacaa tccgctccgg ccacggaaaa
      121 tctttgatcc ggtcgatgaa cggactggcg gaaatggcga cgcgtaactc ggcgcgtcgg
      181 caacaagctt cgttcgccgc tcggccgctc gtgaagttta aatcgacggc gaccttgtcg
      241 gccagggcgg tcgcgagatt tgcgacgact gcgcctctgc cggcgggcag aacgatcgcc
      301 agtcgttgat cgggaaattc tctgcgcaaa aatcgactca gcgcggcggc ggcgccgagg
      361 agcttggcgc ggctcagtct gcttccgtca attccgtcgg tgactgcggt cgcgaatgat
      421 cgccgtttca aaccgcggat gcagtcacgg gcgagatgtc gatcaaacat ggagcggcgg
      481 ctgtaacaaa attcgcccag cttcagcaac tcttcgcgca ctgtcgcgat gttcgccttc
      541 tcggcgtcga gcgccttgcc gaacgcaacg gtaacggggt aggggatgcg ccgcggaagt
      601 ttggtgaaga agcgcccgcc tttgaaggag aagatcgagc cccaaagttg gtcgagccaa
      661 accggaacaa cgcgggtgcg ggcgtgtcgc gcgatcagtt cgtaaccgcg ttggaggcgc
      721 aaaagtacgc cgcggcgttc gagttggcct tccggaaaaa gacagacgat ttcgcccgcg
      781 gcgattcttt ccgtcgcggc ccgaatcgcg ctgcgcggat ggcggatatc cattggaata
      841 cagccaagga cgcgcaggaa tgggtgcagc atcggtttgt ggtaaaactc ttggtcgatg
      901 acataacgaa tcggccgcgg gcaggcggct tgcagcacga tggcgtcgac ccaggtgatg
      961 tggttgggca aaaggagaaa gccgccggct ggcagcgaat caaagccgaa tgtcgcgatc
     1021 cgataagcga cgcgcacaag tggccggccg acaaagtgca gcgtgcgctc gaccgcggac
     1081 atgctcggca tgtttgtgat ttacaatcgg ggcggcgctc actgcaatcg gcaattgtcg
     1141 atggaaaatc gttccggaac aacgcggacg ttcgcgcgct ccgcggtctg gctgctgctc
     1201 gggatcaatt tattcaacta catcgaccgc cagattttgg cggcggtgct gccgcggata
     1261 agacacgatt ttctcgctgg cgatcccaat caaaacagca aagccggtct gctgacgacc
     1321 gcgttcctgt tcagttacat gttcaccgcg ccggtgtttg gctggctggc cgatcggttc
     1381 tcgcgttggg tgcttgtggg gatcagcgtc gcgttgtgga gcgtcgcgag tgggtggtca
     1441 ggattggcga cttcgttcgg gattttgctt tggacgcgcg cgttcgtcgg gatcggcgag
     1501 gcaggctacg gccctgccgc accgacgatc atctccgatt tgtatccgct ggagcgccgc
     1561 ggtcgcgtgc tggcatattt ttacctcgcg ttgcctgtgg gcggcgctct cggctacgcc
     1621 tttggcggaa tgattgccga tcttctcggc tggcgctggg cgttttacct cgttgtaccg
     1681 cctggactac tgctcgccgc gttttgtttt ttcatgcgcg atccgcgtgc cgacgcgcaa
     1741 cgtggtttca gattaggcgc ccgaccacgc tggcaagatt acgtgcgcct tttgcggatt
     1801 ccctcctacg tgctgaacac ggcggctatg actgcactga cgtttgcgat cggcggcttc
     1861 tcagcctggt tgccggatta catttatact gatcggcgcg ccgaattcgc gcgcgatccc
     1921 aatctgctcg gcaagatcga cctcatcttc ggcgcggtcg ttgccgtcgc aggattgttc
     1981 gcgacgctct tcggtggctg ggctggagac tggctgcgga acaaattcaa gagtgcatac
     2041 tttctcgtct cggctgttgg cgtgttgctg gcgtttcccg cgacgatcgc gatgctctac
     2101 gtgcgtttcc ccggggcgtg ggtctgcatt tttgtcgcgg tgttctttct ctttttcaat
     2161 accggcccgt caaacaccgc gctcgcgaat gtcacttcgc cggcggttcg cgcgacagcg
     2221 ttcgcgttga acatcctgat catccatgcg ctcggcgacg cgatctcacc accgctgatc
     2281 ggtgcgattc gcgatcgatg gaacatgaat gccggatttc ttgccgtctc cggtacaatg
     2341 ttgctcgcgg gagtcttttg gctggtcggc gcgcgtttct taccgcggga taccatgaag
     2401 gtagaacggg cgacggaaca aagcgccgct cgtgctgctt gattgtcgat ctaacaaagg
     2461 agccgcagaa gctggtgatg cctctgcggc tccgcgtttc gagattcgaa atctcgaaag
     2521 ctaatctcgt tattcttgtt cctgacttgt ctttggtgtc gcgctcggtt cagccgcacc
     2581 ttcgtgggcg tgcttccgcc cacctttttt gtgctcgccg gcaggactgg ctcctgtttc
     2641 cattccggat gccgctccgg ttgctgactc cgtaccggtg cggcctctgc cagtggattc
     2701 ggtcgcttca cgaccagtca tcgatgactc ggtcgcgcgc gcttccggcg actctgcggc
     2761 gcccggactc atcctttcac ttggcgatgc tcccggagat gccccacgtc gggcaccatg
     2821 cctgcctcgc ggcgaagcag tcgcggctgc ggaagcagtt gcggagggcg aggcgcttgc
     2881 cccaaccgcc ggagtcatac gaggcgacgc actcgcgtat ggcgatgcgg cgccagctgt
     2941 tccagtcgca ccagccgcgc cgggtgaagg gctaccgtag gcacctgccg ctccggtggc
     3001 agctccagtt gctgtggtgg tcgtcccggt cgttgtaccg gttacagaag ttgatactcc
     3061 ggccggcgcc gagacgccat agtaggagta gacgttattg atgtaatcgg gtctcgagta
     3121 ttcatcaatc cgcgcatagt caaacaccgg tgcactgtag atccgctcac gatctatacg
     3181 cacagtgtag acagttgggt cggatgtgac ggaataaacc gtccacggca cggcgactgt
     3241 ttttgcctgg ccggcgacac gcgcagtggt gccgcccgtc gagagaacgg tgtaagccat
     3301 gcagccgctg ttccggtcga gcacgacatc tctaatttca ccaatctctt cgccttgggc
     3361 ggtctttact tttgaaccga tgatcttgct ggtctggaca tacgtcgtcg aggtagacgt
     3421 tgtgctggtt gtttgggctt ttgagacaag cggggcaatg gccaagccca caatggcgag
     3481 caaagccccg agacagaagg aggttttcat aacatctaca gacgtatcgc cgctgcggcc
     3541 tattcaggac gctttcagcg gccacgccgg gatagtgcct taaagaaccg aaggcgaacc
     3601 gagatgagcg tcgtagtcgc gcgcctgagc gagtgcgcga ttggcttgcg tgctgtggcc
     3661 gagtttcgcg tgcaattggc ctaacaactg ccagctctcc gcacgatacc attgcgtttg
     3721 caagcaggtt agcaattcgt taatcgccgc cggtgtcgca cccgtttgat cgagcagttg
     3781 gatgtaacgt ttttcgatcg accaatccgg cggaccggtg cgtgcggcca gtcgcatgcg
     3841 cagaacatcg acacctccgt tttccttatg ttcgagcacg gcgagcaatt cgtgggcttg
     3901 tgcattgacc agcggcatct gcgttgcgcg ctcgagaagt tggcgcgcga gcttgaggtc
     3961 gttctgttgc aaaaggacgg cggcgagatt gatgacggcg agcggttggt ccggtgtctt
     4021 ttgcaacgcg gcccgcaaat gcaacgccgc ttcttgcaac ctgccttcgc tcgcttccaa
     4081 gctgccaagg ttgaccaaca tgcgcaccga atcgccgcct tgggcaattg tgcgcgcaaa
     4141 aaaggtgcgc tggtctttcc agtcgaaggt gcggacaaat gtgcgcgcgc cgaggaaaag
     4201 cagccagagc gtgaacacgc cggcggcaac ggtgactgtc gatcttgcgc aacgcaaact
     4261 accgagctca accgccgcgg ccaaaaacaa gaacgcgctc ggcagataca gccagtgctc
     4321 ggcgaccgtc gcattcaaag cgacgattcc actaatcggc aaatagctga tgagagcgat
     4381 gaggagcagt gcaaaggccg ccggattacg tttccgcgct cgccacgtcc agtagaggag
     4441 tgccgcgatg agcacgatgc caagtaatgt ctgcagttcg cgccacgctg caccgcgcaa
     4501 actggcgtca ttccagccgc tcggatgggt ttcgacatcc cgatccgcgt gtaggttgag
     4561 tggaaacagc atcagtccgg cgtactcggc cacggcccgt gcagcgataa ttggcctaac
     4621 gagtgggggc gcgggcgcgc tcattgccgg aggatggtag tgctcggcgc ccaagcgcaa
     4681 actgaagtaa atcgcggcga taaatgcggc gacagcaact gttttccaca tgtcgatcca
     4741 agctttgcgc acggcaaata acatgagcgc cagaaccgga aagatgagcc ccgcttcctt
     4801 gcagaacgcg ctcaggagaa acgccaagcc ggacccgata aagaaacgcc atctacttgc
     4861 gccggtcgcg cgcgtgctgc ggatcaccag atacaacccg gtgaacccaa atagcgcggc
     4921 aagtggatcg gcccgccctg agacgtaagc gacggctggg atttgcaccg ggtggatggc
     4981 ccagacaagc gtggcgagca acgcaatcag acgccgtttt cggtcgtcca ctccgtaact
     5041 aagaagaagt tcgtgtgcga agatgaaaag cgcgacgacg gcggcggcgt gcgagatgat
     5101 gctcgataga tggtacagca ttggtcgaaa gccgaccgcg acatactcga gcgtgtaggt
     5161 gacacgttgc agcggccgat aaaagtccga tggagtcgcg tcggcaaaga ggaaatggtt
     5221 aaagccttca ggaatgaggc gccagcttcg gatcagcgga tcgcgcaaca ccaaggcggt
     5281 gtcgtcccac acgaagccgt tgccgagcgt gggcgcgtag atcgcggcca cgacgacaat
     5341 gataagtaaa ggcgcgaaag tttttttcac gaaaagcagg tgcagagcat taataaaatg
     5401 cgcgtgtaga tttggagatg tttatgtcga tcttgcgccg cgccgcagtg ctcgtactgc
     5461 tctcgttgac cagcaatgcg tgtgccgccg gtgtgccgga ggactgtacg cagttgatcg
     5521 ttggtctcgc ccctgactgg aactcaatgc gcggcacatt gcagcgcttt gagcgcacgc
     5581 gtggcggcga gtggcgtgct gtcagcaaag cggttccggt attgttcggc aaaaacggac
     5641 tagcgtgggg aatcggtctc gccggtcagc acgaagccgg gcttcacaaa aaggagcggg
     5701 acggccgtgc gccggccggc attttcgaga tcggccaagt cttcggttac gacagcaagc
     5761 tcccgccggg cggtgattat ccataccacc aggtgaccga ggcagacatt tggagcgatg
     5821 acccccgctc gccgaattac aaccggcacg tcgttatcga cccgcagaat ccgcccgaca
     5881 attactcgca tgagaaaatg cgttccggtg atttcgccta ccggtggctt gtcgatgtcc
     5941 gacacaattc caatctgcca atccccggcg cgggtagcgc cattttcttc cacatccgcc
     6001 gcggggtgaa tcggccgacg acgggctgca cgacgatggc cgagcctgag ctcgtgaagc
     6061 tgatcacttg gttacgcgcg aaccagcacc cctgctacgc tcttttgccg gtcgcggaat
     6121 acgagcgcaa atggcaggac tggcacctgc cgccatcgaa aaaagttcgg acagccgtta
     6181 acagccgttg aacagcggcg cgcgttcgct accttctggc tactcccatg ccaagacgtg
     6241 ctaatcccga ctcagaagca aacatccgcc gcatcgacac gcgcatgcgc gcgaagaaac
     6301 aaacgcacgg ttttcaagtt catttcctcc gcggtgcgac ggccgtaacc aaaatgttca
     6361 gcgacacgct ctacggcgga aaagaagcgg cccggcgcgc agcccgaaag ttcaaggaga
     6421 tagccatgcg tggtttgccg cagcgcaatg cggcgggctc gaaatcgaca acgcggcgga
     6481 aaacaaccac tcgcgcgcgg cgacgatcgg ctacaatgcg gcggcgctaa gcacaccgtg
     6541 ctggcgaaac gcgtgccagc attccatgca cgccggtgtg aaatcctccc gccgagcagc
     6601 gatccagccg tctaccactt cgggacggaa aaatccgccg gcttcgattt cactgcgatt
     6661 cggccgcaca gcgttttgaa cgtggccgcg atatagccag atgaactctt ggtccgttcg
     6721 cgtcgaggca ggtagtttca gaagtttctc cagcggcacg tcgatcctaa gctcttcccg
     6781 tagctcgcgc cgcgccgctt catcgtagcc ttccgccgcg ctgacatgcc cgcctgcgct
     6841 ggagtcccac tgcagtggat gtcgatcttt ccagcgtgcg cgtttttgca aatagacttc
     6901 gcctgcctcg ttgaaaatga ggatgtggac agcgcgatgg cgcgacttgt ccccatgcac
     6961 ttctgagcgg taggcgtagc gcaaaatccg atccatctcg tccacgaccg gaaagcgttc
     7021 ttgccagaaa acctgctgct ccgagctgtg gatcggtgcg atttggttgg ccagggcgat
     7081 ccattgctca agcgacagat tttccgcccg cgctttcggc tcgatctcga gcttggccgc
     7141 gatttttggc cagtctgcgg tgaaatcgct gagcgccttg tgcaactgtt tgcgccggtg
     7201 cgtaaaccca cgacgcaaca ggtcgttcag caattcatca tcacatgcag gcagttccag
     7261 cgggtcacgc gggacgattc gcacgagcgt cgacgcgact tccggctccg gaacaaaaac
     7321 cgtagctgga atgctccgca gatgctcgac ccgatggtgc aattgtagcc gcacggtcag
     7381 cgcgccgtac tctcgcgtgg ccggtgaagc agttagtcgc gcagcaactt ctcgctgcaa
     7441 cgtaaacagc cagagtgaga tgggactcgg gtactggaca tatttcgcga gcaacgggct
     7501 tgaaatagtg tagggcagat tcccaagcag tttgacgcgc cgctcacaaa acagcgctcg
     7561 caaatcgaag tcgagcgcgt cttcgttcaa cacttccagc cgcggatgcg cgagatgctc
     7621 gcgcaaaaac gccgccaacc gcgtgtcctt ctcgatcgct agaactcgcg cgccggtgtc
     7681 caaaatgatc gacgtcagcg cgcccaatcc ggggccgatt tccaccacgt aatcctcggg
     7741 cgtgagctca gccgctttca cgatacagcg cgccaggttt tggtcgtgca ggaaactttg
     7801 tccgagcttt tttacgggcg atactcgaat ctcctgcagc gtcgcggtga tgtcagagag
     7861 cttcattgta agatcgacaa tcggttccga acgcacggcg aaaatcgcca agctgttcac
     7921 aacaggcggt gaacaagatg cgccggaaag atcattttat tcacaagtta ttcgatagcc
     7981 aagagcgacg ggggcggctt ctctactcca tcacgcgaag gtcgccccga aaacaaattg
     8041 cgatcaaaaa tctgaactga taatcctgct cgtttcccat gagcctaaac gtcgagattc
     8101 tttcccgcgc cgcgaacgaa gcgcgcggcc tgtgcatgga cgcggtgcaa gcgtcgcaat
     8161 ccggtcacct tgggttgccg ctcggctgcg ccgagatggg cgcagttctt tacggctacg
     8221 ccctcaagca caatccggac aaaccgcgct ggatcggccg ggattatttc gttctatcgg
     8281 ccggccatgg cagcatgttc ctctacgcct ggctgcactt gagcggctac gatttgccga
     8341 tgtcggaaat caagcgtttc cgtcaactac attctaaaac cccgggccat cccgaatttt
     8401 tcgaaacgcc gggcgttgaa tgcacgacgg gccctctggg acagggcgtg ggcaacgccg
     8461 tcggtattgc ggtcgcgatg aaaatggcgg cggcgcgttt caacagtgat gagcacagaa
     8521 ttttcgatca acatgtgatc tgtcttgcgg gcgacggttg catgcaggag ggcgtagcct
     8581 cggaagcgag cgcgttcgcc ggccatttcg gcctcgacaa tttgatcctg atctacgatt
     8641 cgaacgcggt cacgctcgac gcgccggcaa aagagacgca gagcgaagac actggcaaac
     8701 ggtttgccgc ttacggattc gacgtgcagg aaatccaggg cgacgacatg cagcagtttc
     8761 tcgacgcgct caacatcgcg aaagagcgcg acaatggaaa accgcagttc atcattgcgc
     8821 acacgctgat cggtaaaggc attcccgaag tcgctggtac ctacaaggcg catggcgaag
     8881 ccggtgcaaa gtttgtcgac gcgggacgaa agggcctcgg ccttcccgac gagcattact
     8941 acgtgtcgaa agaggtgtac gagtatttcg ccgagcacaa aaagaagctc ctcgccgatt
     9001 acgatcgctg ggaaaaaaat tacgacgagt ggcgcaagaa aaatcccgaa cgcgcgaaaa
     9061 tcctcgacga cgggatcgag cgcagagttc ccgctgatct cttctcgaaa atccccgaat
     9121 tcccgaaaga cgcgaagctg gcgacgcgga aagccggcgg cgaggtcttg cagccgatcg
     9181 cgcgcgagat gccgctgctc atcggcggca gcgccgactt gtacggctcg accatgaatt
     9241 acatcaagga cggcggcgac ttcacacgcg acacgtcggc cggccgcaac atccgtttcg
     9301 gcatccgcga acacggcatg tgtgcgatcc tgaacggaat ttcctatcac ggaatcttcc
     9361 gcgcctccgg cgcaacgttc ctcgtcttca ccgactattg tcgcgcgtcc attcgtttag
     9421 gcgcgctctc gaagctcccg aatacttaca tcttcacgca cgactccatc ggcgtcggcg
     9481 aagacggccc gacgcacgaa ccggtcgaaa cggtcagctc cgttcgtgtg atgccgcaga
     9541 tcgatctgat tcggccagcc gaccccgagg aaaccgccgg cgcgtttgtt gccgcgatgg
     9601 aacgaaacaa cggaccgacg atgatcgcgc tcacccgcca aaccgtgccg attttgaacg
     9661 atgtcgatgt tgcagtgcgg cgcgatggcg tcctgcgcgg cggttacatc gcaaaaaagg
     9721 aaacaggcaa actcgacctc atcatcatgt cctgcggcag cgaattgcaa cacgcgctcg
     9781 ccgccgcgaa acaactgggc gacaatgtcc gcgtcgtttc catgccgtgc ttcgagcgct
     9841 tcgagcgcca gccgcaggga tatcgcgatg aagttttacc caacgaatgt cgcagacgca
     9901 tcgccatcga agccggggtg cccgacacct ggtttcgata tgtcgggctc gatgggaaag
     9961 tcatcggcct gcaccgcttc ggcctcagcg cccccggcgc cgaggtgatg aaggaattcg
    10021 gcatcgacgc cgatcacgtg attcaagcgg cgaagtcgtt gtggtagggc gcgcggctcg
    10081 cgcgccgcgc ccgatgtctc gtcgagcgca attgtgctcc gcgagacgcc cgcgctaccc
    10141 agatcgacgg gctaaaacgc ttcttccggc tcggtgacgt tccgggcgcg atcttcgaat
    10201 cgcgtgaatt ccttcaggaa ggtaagcgca atctcgccga ccggaccgtt gcgttgtttc
    10261 gcgatgatca attccgcttc gccggccttt tccgcgcgcg cttcttcgtc ttcctcgtaa
    10321 atctccggcc taacgagcaa cccaacgagg tcggcgtcct gttcgatcgc accggattcg
    10381 cgcaaatcgc ttagccgcgg tttgccgccg gagcgctgct cgggctggcg attcaactgc
    10441 gccacgacga taaccgggat cttcaattcc ttggccaatc ccttcaggcc agcggaaatt
    10501 tccgagatct cgagctgccg attgtcctgc gcgcggcgtg atgtcgatct tagcagttgc
    10561 aaataatcga tcaccagtaa ttgcacatcc tgctgcgcct tgagccgccg cgcttttgcg
    10621 cgcaacgcca gaatgtcgag tgccgcactg tcgtcgatga agatttttgc ttccgccagt
    10681 ttcgccgcag ctgcggtcag acttggaaaa tcgcgctcgg ccaggaatcc gtcgcgcact
    10741 ttctgcagat tgacgcgagc acgggagcaa agaagacgct ggacgagctg ctgaccgctc
    10801 atttccaaac tgaaaacgcc gactgggagt ttttcgttga tcgcgacatg ctcggcgata
    10861 ttcatcgcga gcgccgtttt gcccatgctt ggtcggccgg cgatcacgat catctcggcg
    10921 gcgtgcatcc cgctcgtcat ccgatcgaat tcgacgaagc cggttgaaac accggtgatc
    10981 ccgcctttgc gttcgtagag tttttcgatt gcctcgatcg cttccatgac ctggtctttc
    11041 atcgagagca tgtgcccttt gaaccggtcc tcgccgacgg cgaaaatttt ctgctccact
    11101 tcgtcgagca gattgctgac ttcatcctgt tcctcgtagg cgcgccgcac gctttcggtt
    11161 gccgccgaaa tgatttcgcg cagaacaaac ttgtcccgga cgatgtcgag gtaatagccg
    11221 acgttggcgg ccgtgggcac aaacgtgaac aggctcgtga caaacgccgg gccgccgacc
    11281 gcgtcgagca aattccgatc gcgcaacacc tgggtgaaag taatcaggtc gatggcttgc
    11341 ccagtgttcc aaagatcgac aagcacgttg tagattgtct gatgcgccgg gacgtagaaa
    11401 taattctcgt taatcttttc gacgcactcc gcaatcgcgt cgcgtggcga aatcagcatc
    11461 gaaccgagca cgccctgctc ggcttcaacg ctgtgcggcg gcgtccggtg aatatcctgc
    11521 gaagaagctg tgagtgactt gccgccgtcc gacccgccgt ttcctcccgg ccagcctcgg
    11581 ccgggctttt tacccgattg cttgcgaggc gtctctccag tccaagcggc tggctgagtc
    11641 gccatttatt tccctggctc ttttctctct tcttttttcc tgcggaaaaa cgagcggtgc
    11701 tttttctccg gctcttccgc ggcagtttgc tgcggcttcg gctcttcggc cgatttcacg
    11761 tcgaatttga aatgcgccgt cacatcatgg tgcaacttga tcgcgacttc atgctccccc
    11821 gtgtctttga tggggcgctc gagcacgatt ttgtggcgat cgatttcgtg gccgatctcg
    11881 tttcgcaatc ggttcacgat gtcctgcgcc gtgacggatc cgaaagcctt gcccgtttcg
    11941 cccgtcgcga gcgtgaacgt cacgcgcgtc ttgccgatgc gccgggcaat atcttccgcc
    12001 tcgttcagct cgcgcgcttc acgctcggcg cgtttcttct tcagattatc cagctggcgc
    12061 aacgctgccg gcgtcacctc gtaggctttg ccattcggca gcagatagtt gcgggcatag
    12121 ccgcgacgca ctttcactag gtcggcttcc gcgccgagtc cgggaacgtt ttcggtcaaa
    12181 atcacttcgg tcgagggcat gtcgatcctg caacaatttg cgctcgcgaa aatttcggcg
    12241 agcgacgcgt catcaaagca gcgtgctcac tgcctgtcaa cgtgcactag aaaaaacttc
    12301 tgaagacgcg aattcgtgac gggaaaattg ccagtccggc ttggactcag atcatgccga
    12361 gcttcatgcg cccggcttgg ctgatcatgt tctggttcca cggcggatcc cagacgagct
    12421 gcacgtcggc ttcgtccacg ccgtcgatcg acaaaatttt cgcctgcgcg tcgtgcgcga
    12481 tggccgggcc catgccgcaa cccggcgcgg tcagcgtcat cttcacctcc acccgcgtgc
    12541 cgccgtcatc ttttttgact aaccgacaat cgtaaaccaa cccaaggtcg acgatgttca
    12601 ccggaatctc cggatcatag cactggcgca attgattcca gacatcttcc tctgagactt
    12661 cgccgttcgt tcgactcgcc ggcgtcgttt cagctctggc ctgcggcttc tcgaggccga
    12721 gcgcgtccac atctttctcc gcgatacgcg acaatccgta cggcgtcgca atcgtgtaac
    12781 tgccgccgag cgtctgcgtg atcaccccct gcgttccgtt ctcgagcgta aatttgcgtc
    12841 cgctcggaat ctcaatcgcc tccacgtcgc ggcttaagct gaattccgtg ttttcgtaca
    12901 ttgcggttgc aacctaagcg cccgcatcgc gaccgcaagc attgtagagc cagctgtgtc
    12961 agccgcctgt ttacagctgg cgcttggcac aagcgccgct acaactgctt cggattctcc
    13021 aacaattcga tcacgcgctt cagcaaacgt gcgccgccgc cgccgtcgat gctgcgatgg
    13081 tcgaagctca atgtgatctg tgcttcggtt ctcgggacga attctttttt tgattcgtcc
    13141 cacgatggaa gctttttgcc cgcgccgaga ccgagcagca gcgtttgatc gggcaggggg
    13201 atgggcgtgc cccattccac gccaaagatt ccgaaattgg aaactgttgc gatgccgccc
    13261 gcctgcatgt ccggcgagat ccgccgtttg cgcgcaagct cgacaagttc ctggtatttc
    13321 gccgtgagct cgacgagcga cgttttgtcg gcggaacgaa tgactggaac catgattccg
    13381 tcttcggctt cgacagcaat gccgacgtcg atggagttgg attggatgac gcgattgccg
    13441 atcaatttcg ccgcggcttt cgggttctcc gcgagtgcga gcgcgagcgc gcgcagcgcg
    13501 taaagtcccg gcccaggctt cggctcgcgc gattgtcgat cttccaaaac ggggtcgaga
    13561 ttcactgacg agccgaccgt agatagcggc cgactccaac tgcgccgcat cgcgtccgcg
    13621 acgccggcac gaatcgcgga agcttcctgc gccgtttcgc tgtcgatctt gtgcagaaaa
    13681 ttttccagat ctttgatcgt cacgcgattg cccgcgcccg tcccggtaat tccggcaaga
    13741 tcagcattcg tcaattggag ctcggccatg cgcgcgcgca cccgcggcga aatgaagctc
    13801 gcgcctttca aggttgcggg aacaggcaga gtagggcgac cgtcccggtt gccatctccc
    13861 actggcaggc gggacggttg cgctaccttc acttcctggt ccgcgcgcgc gggaatttct
    13921 tcggccaccg tccgctgcgg tggagccggc gcctgtttcg tgaatcgcgc cgcgtcagcc
    13981 tcgctcgctt ccacgtaacc cagcacggcg ccgatcggat aggtctcctt caattgggcg
    14041 gcgatgttgt cgatcttgcc tttgcacggc gtggtcacac ccatgacagc cttgttcgtt
    14101 tcgacgtcga tgatttcttg atcggctgcg acttcgtcgc cgggcttcac cttgatatcg
    14161 acaatcgtcg cttcggcgat cgattcaccg agctgcggca tgatgatcgg gacctgtggc
    14221 ataaaagtta ctcgcgaaga agttgtcgcg ctttcgcgac gattttctgc gcgttcggcc
    14281 gatgcgcgct ccaaagattc ggatgatacg ggatcggcgt gtctttcgcg ttgattcgca
    14341 gcggcgcggc atcgagcaga tgaaaacctt cgctggtgac gcgcgcgatg acttccgcag
    14401 tcgcgccgcc ccaagggaag gattcgccaa cgcaaagtaa tcgcgccgtg cgcgcgactg
    14461 acgccagcac cgtgtcggtg tcgagcggtt taaccgtgcg aagatcgaca acttcgattt
    14521 ggagaccttc gcgtgcgagt tcgtccgcag cggtgagcgc ttcctgcacc attacgctgt
    14581 aagcgacgat tgtaagatca tggccttcgc gcgcgattcg cgccttgccg atcggcaatc
    14641 cttcggtcgg cagcttgtcc gctttgaggt gataatacag atacttgtgc tcgcaataca
    14701 cgaccgggtc atcgatcccg accgcatcga tcaacattgt gtacgcatct tcgacggtcg
    14761 ctggtgtcat cacgatcagc ccgggataat gcgcatagat cgattccatc gactgactgt
    14821 gaaacgggcc gctccctttc gttccgccgg aggggagacg aatcgtgatt ggaaccggca
    14881 cattcgtccg ccagtactgc gtgccggcgt tgttcagaat ttgattgagc gcggtactcg
    14941 aaaaatcggc gaactgcatc tcgacaatcg gccgcatccc atgcatcgcc gcgccgatcg
    15001 cggtgccgac catcgcgtct tcgctgattg gagtgtttaa gacgcggccg ggaaattctt
    15061 gggcaagacc tttggttgcc ttaaacgcgc cgccaaacgt gccgccgacg tcctgcccgt
    15121 aaatgaagac gcgctcgtca tctcgcaaca gtttcgcctg cgcttcgcgg atcgcctcca
    15181 gataggtaat gctcatggct ttgccacgtt aaatcgttaa atcgctgaaa cgttgaattg
    15241 gaaacatcat tcaactgatt ccgcgtgatc agcgagcgcg cgcgtcgaaa tcgcgcacca
    15301 atcttcttct gcgccgtccg gcgccggctc cttctgcgcg gtcgcgatcg cttcatcgac
    15361 ttgcgccgcc gcatcttcgc gccacgactg caacgttgcg ggatcgagta actcaagatc
    15421 gacaatcttt ttctcggtca atttcaaaca atcctgtgca aagggctggc gtttgatttc
    15481 atcagttaca taactcgcgt catcgtgttc gccatgaccg gacaaacgca aagtagaggc
    15541 gaccaccaac tgcggcgggc gaccggcgcg agcgcgtttg accgcgctct cgatgacttc
    15601 gaggcactgc gttaagtcgg tgccgtccag cttgtagcct tcaaagccgt agcctttcgc
    15661 gcggtccact aattcctgac aagcgaactc ccgttcgtta ggcgtcgaat aggcgtaatg
    15721 gttgttggtt gcgacaacga cgagcggcac ttgttcgact gcggcaagat tcattccctc
    15781 gtgaaacgag ccggtctgca tcccgccttc gccgatggag gtgagtccga tgaagttttt
    15841 ttcgccgcga aaacgcttcg ccattagtgc acctacggta accgagacca tcgcgccgag
    15901 atgactgatc atcgcgagct ggccctcgcg tggcttgccg cgatggatgt taccgtcgcg
    15961 tccgcgcatc gggccgtgtc gcgagccgag atacgttcgt gccacatcga ccagcggctc
    16021 gccgaaagca gagcgacccg cttggtcgcg aatcagcggc gcaaagatgt cgcccttttg
    16081 cagaaacaaa ccgcacgcta cgctgaccgc ctcttggcct ttgccgatgt aaacgccgcc
    16141 ggttatttgt ccgccgcgat agagactgac cagcttttcc tcaagcgtcc gcgccagcag
    16201 catccagcga aatgcttcca aaaatcgctc gtgcggcgcg gctttcttca aagcggattt
    16261 tttgctgcgc gccgccgtct gaagcatccg ccaattttac gctagatcga caaacgcgcg
    16321 caaaaattat ttggaacata gacttttagt ctgtgcggcc agcgggtttg taacccgctg
    16381 aataaacagc ggacaatatg tccgctggcc acacaggctg aaagcctatg ttccgttatc
    16441 gcgcggcgaa gcttgactct gtttcgctgc gcccgttttc ggccaaagca aattgccgct
    16501 gcgaagaaac agtttcgccg gtttccggct gcgatttatc ggccggtgcg tctatcagtt
    16561 tcatcgtgac cagtttgctc acgccgcgtt cttccatcgt gacgccgtaa agcacgtcgg
    16621 ctttggcgat cgtccgtttg ttgtgtgtaa tgatgatgaa ctggctttgg ctaacgaatc
    16681 gctcgagaat cgcgaggaac cggttgatgt tggtttcgtc gagcggcgcg tcaatttcgt
    16741 ccagcacgca aaacggactc gggcgtacca tgtaaatggc aaacagcaat gcgatcgcga
    16801 ccatcgagcg ttcgccgccg ctcaacagcg agatgctttg cagctgttta ccgggcggct
    16861 tggccgtgat ctcaatacca cagttgagcg ggtcgctctc gtcgagtaac gacagatcgg
    16921 cccggccgcc gccgaacatt tcggcaaaca tttcgcggaa attcccacgg atctgggtga
    16981 aagtttcagc gaacatttgt tttgtcgtcg cgttgatccg cgcgatgact tcgagtaatt
    17041 cgcggcgcga gtttgtcagg tcggtgttct gcgcctcaag gaatttgtag cgttcttcca
    17101 gctcatcgta ttcctgcacg gcctcgagat tgacggggcc catgttgtca agctgctgtg
    17161 taagatcgac aatcagcttc tgcagatctt cgtcgctgag atcggaagct aggtggatcg
    17221 cgctctccga gcgcgatgct aaatccgacg cggcttcgcc gccagtatta gcatcgcccg
    17281 cggaccggtc gatccacctt ttgaattgcg cgcgcaatgt tttctcgaat acggcttcgt
    17341 ccggcgcggc cgcacggaga tcgaaatgat actgacgccc gacgcggtcg ccgagatgct
    17401 gaatttccat ccggagctgc gactcgcgga cttgctgttg cgcgcgatcg ttttggagat
    17461 tgctgagtga atggcgcagt tcacgcagct ccgtttcccg ccgttcgacc tccgccgtcc
    17521 gcgtcgcgcg tctttggccg agcgccgcgc cgtatgtttc taactccatc gcgcgcgtcg
    17581 tttgctcggc gatcagtcgt tcggcttggt gagactcgtc ggcttgggca gccattttgt
    17641 tttcgtagcc gacgatatca gcgcggcgcg cagcaatgag atccgtgagt tccaggtcgc
    17701 gcgcgcgtat cgcttcgcat tgggcgcgca ggttctcgta gcgctgctgc tccgtagcga
    17761 gcgcgagttt cagctcggtg atctgttcgc gtgcgcgctc ctcttgcaaa cgcgcttcgt
    17821 tctcgtcctg ttcgaccgaa gcacgatcgt tttgccgccg gtcgagttcg cggcgcaatt
    17881 cacttaggtc agtctcaatt tgcgcgacgc gttcgtcagc cgacttaatt tgctcctcga
    17941 gcgttcgcgt atcgctctgc aaattgtcga tcttgcctgt gactgtctcc tgctcggttt
    18001 cgagcaggga gattctgccg gccgcggtcg agttcaccac gtgcgcagcc tgttccgccg
    18061 cgcgggtccg ctcgagctca tgtgacgctt tgtcgagttc ggtccgcaat tcttcacatc
    18121 gctcgagtag cagctcgcgt tggccggcga gcgcgttttc ctcatcagcc aacgctgtga
    18181 cccgggcttt gcggtcgagc caggaatcgc gacgcgctac gttgcttccg ccgaaaataa
    18241 cgccttcggc agaaatgaat tcgccatcga gagttgccat cgcgagagcg ggctcttgct
    18301 ttttacacgc gagggcgcgt tccaggtccg cgaagatgac gacctcgctg agaagtcggc
    18361 ggatgagcgg ttcgaaggcg cgcggcgccg tcactttgtc cacggcccag ccgatcgcac
    18421 cgggcggcaa agtttttcgc accgactccg gcgcggaacc gccgagacgc ggcacacaga
    18481 gcgcggcttg tccgaatttc tttttcgtta ggcgtgccac gatctcggcc acgctgccgc
    18541 cgtctttcaa gacgacggca tgcaaatttc ggccgagtgc gccttcaatt gccggcacga
    18601 agcttcgctc gacgtgaagc tgcgcgacga gcggcccaac cacggtgtct cgaaaacgct
    18661 cggggtcgtc cattcctctg agcacggcct gcgaaccctg ctcgagccct tcgccctgtt
    18721 ggttcaactg gcgcaacact tccaatgcgg atcttttttc cgccagcgcg cgatcgatgc
    18781 tggcccagtc gctttccgca cggctgagga gttctcgttc tcgctgcaag cttttttccg
    18841 cggcacgagt ttcagccgaa cgccgcgcga gtgtttgttg ctcttggtcg gtttggctgc
    18901 gtgccgcggt gatctcagcg cccaaacgct cgcgcagtgc gatcgtccgg ctaatttcct
    18961 ccgcgagatg ggcgagttgt tgagatgtaa cttcgcgccg tgcgttcaga ctggagaggt
    19021 cgtcttgcag cgaagcgatg cgctgttcga gtttcgcgac cgattcgcgg acatcggtgc
    19081 gctgtgactg acgcgcttcg cgctgcgctc ggatcgatgc caggaactcc gttgccgctg
    19141 agagttccgt ctccttggct tgcaatattc ctcgtgtatt ggcgaccgcg ccgtcgagtt
    19201 gctcggcttg gcttgccagc tcgcgctgct tcgcttccgc ggcggcgatg tcgttgcgcg
    19261 cgcgctcgat cagctccgcc agctcggctg cgcggagccg gttgaactcg atccgactgt
    19321 gccgcgcggt gatgtcactt tgcacccgct gcgcttccgc ccgcgcgtcc gcgatttcga
    19381 tgtcgatttt gtcgagcgcg tggcgctcct cagcctgtcc ggtttcgccc gcgtcgatct
    19441 tagcacgcgt ttgctgctcg gattcgacaa tgcggtcgat ctcggcgcga cattgcgcga
    19501 gtccggcttc gagcgcggcg agctgtttcc tcgaataatg caggtcgagc acgcgcagat
    19561 cggcatgcag cgcctgatag cgtcgcgcct tacccgcctg acgttgcagt gaaccgatct
    19621 gccgtttcac ttccttgatc acatcaccga tgcgcaacag gttcgcttcg gtcgcttcga
    19681 gcttgcgcaa cgcctcgcgc ttctgcgctt tgtatttcgt gatcccggct gcttcttcga
    19741 aaacggcgcg ccggtcctcc ggatgcgagc tcaagatcat gtcgatcctg ccctgctcca
    19801 tcattgaata agcagcgcgc gccacgccgg tgtcggcgaa aagattttgg atgtcgcgca
    19861 accggcatgc ggttttattg agcaggtatt cggaattacc gtcgcgatag acgcgacggg
    19921 ttacgcgcac ctcgttccac tcgacaccga gttccgcttc gcaattgccg aaggtgacgg
    19981 agacttccgc gaatccgagc ggtttgcgcg tgtcggtgcc gttgaagatg acatcggcca
    20041 tttcgccgcc gcgcagcgac ttggccgatt gctcgccgag cacccaacgg acggcgtcga
    20101 gcaagttgga tttgccgcag ccgttcggac cgacgatggc ggtgacgcct tgatggaagc
    20161 tgaaaaccgt tttgtcggcg aaggatttaa agccgaacag ctcgagtgat tgtagatgca
    20221 tccgccgccg aaaacatcgg cttaggcggg gaatatagcc agcgcactgg cgtgacaagc
    20281 gaaaaagcta ccaaatgcgg tggtcgggac taacatcgag cgcaagattt tgggccacgc
    20341 gctaggtaac ggcaaaccgg ccggatactc gtcgcaacat cgacatcttg ctcgcccacg
    20401 ctaagcttca ggatatgcgt tcggaccagc tcgaagacta ttacacgttt ctgcggttcc
    20461 caagcatttc gaccgacagc gattacgctg cgaaggtgag cgagtgcgca cactggctgg
    20521 tagagaaatt gagtagtgtc ggcttggaag ccaagcttat gccgactccc ggtcatccgg
    20581 ttgtttgggc gcggaacgaa caccgtccgg accggcgcac ggtgctcatt tacggccatt
    20641 atgacgtgca gccgccggat ccattggagc tgtgggaatc gcctcctttc gagccggtgt
    20701 tgaaagacgg ctacgtgttc gcgcgcggcg ccactgacaa caaaggccaa atcctctcgc
    20761 acgttctcgg catcgaggag gcgcttgagc gggacaagga cttgccggtg aatgtcgatc
    20821 ttgtgatcga aggtgaggag gaaatcggga gcgcaaatct tggaaatttt ctcagccaga
    20881 atcgcgaggc gttggagtgc gacgtagtgg tcgtctccga taccggcatg atcgcgccgc
    20941 ggacaccgac gatgacttac gcgttgcgcg gagttgcggc ggtcgagtta aaagtcacag
    21001 gaccgaagat cgatttgcac tccggaattt atggcggtgc ggtggcgaat cctctcgccg
    21061 cgatgtcgcg attgctggcg agcttacacg accgcgatgg gcgagtcgcg atcgcgggtt
    21121 tttacgacga cgtcgaacca ttgcaggatt gggagcggga gatgtggagc aaacttccca
    21181 tcgacgccga cgcgatggtc ttaggggaga gcggagcgcg cgagctgttt ggcgaaaaag
    21241 gttttacgac attggaaaga atttgggcgc gtccgacagc ggagatcaac gggatgggcg
    21301 gcggttatca aggcaaaggg acgaagacag tgttacccag ttacgcgttc gcgaagctga
    21361 cgtttcggct ggtgccgaat cagaatggcg agcggattgt cgatcttgcg cgtaaacact
    21421 tcgagaacaa tttgccacca ggtgtgacgc tggaggtgac gagcggtcac gccggtccct
    21481 ggtatcgaac tgacccgcat aatcggttcg gacaggcggc acaacgcgcg ctgcgcaaga
    21541 ccttcgacaa agaaccggtg ctgattcgtg aaggcggcag catccccatc gtctcccagt
    21601 ttcgcgacat tctcggcgta gaaacgttac tgctcggtct ggcgctgcac gattgccggg
    21661 cgcattcgcc gaatgaaaac tttccgctgg agaatctcga cgctggcatc cgaatgaatc
    21721 aagctgtgct gcgggagttg gctgcgattg gttaggcaaa caagggatga aactggcgag
    21781 gcgttggttc gtggcgatgg cgctgccgct agtgcttacc gttagcgcgc cagaatgcgg
    21841 cgccgcacaa gacgacagcg tgatcgacaa gatcaaacac ctgttcgtca aaccgacgcc
    21901 aacaccgacg ccgaagccgc gcaagaaacg aacaaaaaag aaaagtagcc cgacgccttc
    21961 gcctacccca tccgcttctg ccgccgcggc cgcgaccgcc tctccggctc cgagcgagag
    22021 cgcaccgatc gtgcctgcgg caactgcttc tcccgcagcg agtgcgacgg cgacggcaac
    22081 tcccgctgct gctgccactg tgccgccgcc gagcccttcg cccacaggtt caccgctccc
    22141 gcgacacggc cggtcccagc atctggaacc ggtgcggcca atcgctcctg ggccgcacgc
    22201 gaagtttcgg acaagcgttt caccgccccc tgaagcgtcg ccgcctgcaa ttcgcacgcc
    22261 actgccattg acctcgccgg tttctgcggc aacggcttca ccgctgcccg cagccactgc
    22321 gtcgcgagct gcgacgcccg ccgccaaatc gacgcatgcg ccgaacgcga cgatttcccc
    22381 agacgagatc agcgggtatg aaagttatcc ggccgaagta cggcacgtgc tcgactcagc
    22441 cctcgctcta acgacgcaaa acctcggcta taaatatggc tcggctgacc cggcaaacgg
    22501 tgggatggat tgttccggat tcatctatta cgtagtctcg caatgcggta tcaaagacgc
    22561 gccgcgcgac gcgcgcgagc aatacatatg ggtgcgtaaa gcgggaaatt tccaggccgt
    22621 gctcgcgcat cgcgatgaca ccttcgagct cgacgcgttg aaaccgggcg acctactctt
    22681 ttgggccggc acctacaacg tcagccgcga cccggcaatc acgcacacga tgatttacct
    22741 cggccgcgag aaaggaacga atcagcgcgt catggtcggc gcgagcgacg gccgcaccta
    22801 tcgcggccag cagcggtttg gcgtcagtgt ttttgacttc aaag
//
LOCUS       DASTDG010000064         8018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1173270, whole genome shotgun sequence.
ACCESSION   DASTDG010000064 DASTDG010000000
VERSION     DASTDG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173270"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VFO30_01945"
     CDS             <1..163
                     /locus_tag="VFO30_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9418077.1"
                     /translation="IVQYADNRKGMTMADALAHEIKDAASGQGNAIKKREDTHKMAQA
                     NRAFAHFRW"
     gene            169..2352
                     /gene="fusA"
                     /locus_tag="VFO30_01950"
     CDS             169..2352
                     /gene="fusA"
                     /locus_tag="VFO30_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9418078.1"
                     /translation="MTTATLEKKPAAGAKNPNSPDRKFPLERTRNIGIAAHIDAGKTT
                     LTERVLFYTGMIHKMGEVHEGTTVTDWMEQERERGITITSAATTCAWAQRKEEGVYKI
                     FEGIKQRINIIDTPGHVDFTAEVERSLRVLDGAIAVFDGVAGVQPQSETVWRQATKYN
                     VPRIAFINKMDRVGADFAMSVDSIHKKLGANAWPILLPLGREDQLKGQLDVINKKAII
                     YSDDDELGSTYEVTDVPKEYVDLVDKAYHDLVEQVSNIDDEVAEAVLEERQVTPELLK
                     AGIRRQTIANKFVPVVGGSALKNIGVQYLIDAVVDYLPSPLEIPPAKGQNPDTNEPME
                     AVTDDNGPFCSIAFKLWSDPFVGKLVFFRVYSGTLSKGDTVYNPRTNKRERISRLIQI
                     QADKREDIETCYSGDIAAIVGIKNITTGDTLCDEDHSILLEPPSFPDPVISMAIEPKT
                     KLDQEKMATALQRLSEEDPTFWVYTHEDTGQTIIAGMGELHLEIIRDRMFREFKVDAN
                     AGKPQIAYRETITSSAHGVGKLIKQSGGRGQYGHVEVDVRPTERGKGLVVENKIVGGI
                     IPREYIPAVKKGIEEAVLNGVLGGYPVVDVEVDIVYGSFHEVDSSELAFKMAAIFAMK
                     DGFKQGKPILLEPIMKVENITPDEYQGDICGDLSRRRGSIQSIETRGNMAIIHALVPL
                     AELFGYATAIRSLSKGRSSYSMEPSHFEPVPANLVAAILDQKETK"
     gene            2349..2660
                     /gene="rpsJ"
                     /locus_tag="VFO30_01955"
     CDS             2349..2660
                     /gene="rpsJ"
                     /locus_tag="VFO30_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9418079.1"
                     /translation="MTNGQRIRIRLKAFDHRMLDQSAVDIVETAKRTGARVAGPIPLP
                     TLIEKFTVNRSPHVDKKSMEQFEIRTHKRLLDIIDPTAKTVDELKKLNLPAGVDITIK
                     I"
     gene            2689..3399
                     /gene="rplC"
                     /locus_tag="VFO30_01960"
     CDS             2689..3399
                     /gene="rplC"
                     /locus_tag="VFO30_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9418080.1"
                     /translation="MSIGLLGQKIGMTSVYDAAGKLHPVTVIAAGDNVLLRRLTNEND
                     GYWAVQVGFDGQKESRVNKAELGAFKKAGVEPKKFVREFRLDADLPEGEVNLSVTQFQ
                     PGDFVDVTGRSKGKGFQGVMKKHNFHGQGASHGSKTHRRSGAIGNRSTPGRIWKNMGM
                     PGHLGDERVTVQNLQIMQVREAEKIILVSGAVPGSNGSYVIVRPALKNPAVRIAAHQA
                     HAKEDKAAAAKKPAITVR"
     gene            3403..4122
                     /gene="rplD"
                     /locus_tag="VFO30_01965"
     CDS             3403..4122
                     /gene="rplD"
                     /locus_tag="VFO30_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9418081.1"
                     /translation="MSAKVLSKAAAKEAKIEIIEDGRGTQAVHDVVVAMRAARRSGSA
                     NTKTKAEVDLSGAKPWRQKGTGRARAGYKSSPIWRGGGVVFGPKPRDYSKKTSKSLRR
                     LAFQKALSERINAGDVLTIDKFVVPEIKTKSFISLLKKATDAGKVLVIADSFDEKTFK
                     SARNVKTAHLATAADVNTEQLLAFEKILVTQAALGRLAERTNPPSPGASRLPSHSSAE
                     AGPPLPQGEDRVRAQPKETAK"
     gene            4119..4400
                     /gene="rplW"
                     /locus_tag="VFO30_01970"
     CDS             4119..4400
                     /gene="rplW"
                     /locus_tag="VFO30_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9418082.1"
                     /translation="MIEAHDIIQTASLTEKSTLMSEKQNKYVFRVHPRANKIQIKQAV
                     ERLFQKKVVDVNTCNYAGKWARVRGPIGRKASWKKAIVTLKEGEKIDLV"
     gene            4539..5387
                     /gene="rplB"
                     /locus_tag="VFO30_01975"
     CDS             4539..5387
                     /gene="rplB"
                     /locus_tag="VFO30_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9418083.1"
                     /translation="MALKSFRPLTPVLRFKQLPVFDEITKSRPAKSLLEPKKKSGGRN
                     NFGRLTSRHIGGGHKQHYRKVDFKRRKHGVTAEVIGIEYDPNRSARVALIKYADGELS
                     YILAPNGLGVGSTVAAGPDVPPDLGNALPLRAIPLGTNIHNIELSPGRGGQIARSAGQ
                     QATLSNLEGDYALVKMPSGEVRRIHANCFATIGQVGNVDHMNVSAGKAGRSRWLGRRP
                     HVRGMAMNPIDHPMGGGQGKSKGGGGRHHPVSPWGQLAKGFKTRSKHKPSDRFILERR
                     RSKKKA"
     gene            5460..5738
                     /gene="rpsS"
                     /locus_tag="VFO30_01980"
     CDS             5460..5738
                     /gene="rpsS"
                     /locus_tag="VFO30_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9418084.1"
                     /translation="MARSLKKGPFVESHLLEKIDKMNNAGAKKAIKTWSRRSMVTPDF
                     VGHTFLVHNGKTFQSVFVTENMVGHKLGEFSPTRVFKKHGSHTAKVTK"
     gene            5811..6527
                     /locus_tag="VFO30_01985"
     CDS             5811..6527
                     /locus_tag="VFO30_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418085.1"
                     /translation="MSESKIEDAEHAVTAEALFAQNEELRRQLSIEQESVKTLTMSLA
                     ESNAEAELFRRKFSDLNLRMEALGLASASKDRAKLEQRLLAAVSDLQLAQKERDEYRD
                     QMLRLNEAMLRYLKSSQGGDAQARMDVEAQLRGTNALVTKSTSARPDAQPSLLDGKVI
                     SVKDEWSFVVANLGEKHGVKIGMPLRVMRGEQTIATLRVVDVRQQICGAVIEEMDSNK
                     EKIKVGDRLQVDAKPSVSVR"
     gene            6622..6948
                     /gene="rplV"
                     /locus_tag="VFO30_01990"
                     /pseudo
     CDS             6622..6948
                     /gene="rplV"
                     /locus_tag="VFO30_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            7111..7803
                     /gene="rpsC"
                     /locus_tag="VFO30_01995"
     CDS             7111..7803
                     /gene="rpsC"
                     /locus_tag="VFO30_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9418086.1"
                     /translation="MGQKVNPIGFRLGVNRDWRSRWYASPQELPAFLHSDLEIRHYVK
                     KKLQFAAVSKIVIERAWNSIRVTIFTARPGIVIGRKGAEIEKMTEEIAKMAQGKQIKI
                     DIQEIKTPELDAQLVAENVALQIERRIAYRRAMKKAVQTAMDFGAQGIRLRCSGRLNG
                     AEISRSEWYREGKVPLHTLRTGIDYGFAEANTVYGKIGVKCWMCKKEEAPAEAPTPPA
                     PPPPPEPQPTEV"
BASE COUNT         2008 a         2108 c         2472 g         1430 t
ORIGIN      
        1 gatcgtgcaa tacgccgata atcgcaaagg catgacgatg gccgatgcgc tcgcgcacga
       61 aatcaaagac gccgcgtcgg gtcagggcaa cgcgatcaag aaacgcgaag acacccacaa
      121 aatggcgcag gccaaccgcg cgtttgcgca tttccgctgg taacgtttat gacgaccgcg
      181 actctcgaaa aaaaacccgc ggccggcgcg aagaacccga attctccgga ccgtaagttc
      241 ccgctcgagc ggacgcgcaa catcggcatc gccgcgcaca tcgacgctgg taaaacaacg
      301 ctgaccgagc gcgtcctgtt ttacaccggc atgatccaca agatgggcga ggtgcatgaa
      361 ggcacgaccg tgaccgactg gatggagcag gagcgcgagc gcggcatcac aatcacttcg
      421 gccgcaacga cctgcgcgtg ggcgcaacgc aaggaagaag gcgtttacaa aattttcgaa
      481 gggatcaagc aacgcatcaa catcatcgat acgcccggcc acgttgattt caccgccgaa
      541 gtggagcgct ccttgcgcgt gctcgacggc gcgatagccg ttttcgacgg cgtcgccggt
      601 gtgcagccgc aatccgaaac ggtctggcgg caggcgacga aatacaacgt cccgcgcatc
      661 gcatttatca acaaaatgga tcgcgtcgga gccgacttcg ccatgtcggt cgacagcatt
      721 cataaaaagc tcggggcaaa tgcgtggccg attttgctgc cgctcggacg tgaagatcag
      781 ctcaaaggtc agctcgacgt gatcaacaag aaagcgatca tctattcgga cgacgacgag
      841 ctcggatcga catacgaggt tacggacgtg ccgaaggaat atgtcgatct tgtcgacaaa
      901 gcttaccacg atctggtcga gcaggtttcc aacattgacg atgaagtggc ggaagcggtg
      961 ctggaagagc gccaggtcac gcccgaatta ctcaaggccg gtattcgtcg gcagacgatt
     1021 gcgaacaagt tcgtgcccgt cgtcggcggc tcggcgttaa aaaatatcgg cgtccagtat
     1081 ttaatcgatg ccgttgtcga ttatctgccg agtccactcg agattccgcc ggccaaaggc
     1141 caaaatcccg ataccaacga gccgatggaa gcggtcacgg acgacaatgg gccgttctgc
     1201 tcgatcgcgt ttaaactttg gagcgatccg ttcgtcggca agctggtttt ctttcgcgtt
     1261 tactccggca cgctttcaaa aggcgatacc gtttacaatc cgcggacgaa caaacgcgaa
     1321 cgcatctcgc gtttgatcca gatccaggcc gacaaacgcg aagacatcga gacctgttac
     1381 tcgggcgaca tcgccgcgat cgtcggcatc aagaacatca cgactggcga tacgctttgc
     1441 gacgaagatc attcgatcct gcttgagccg ccgtcgttcc ccgaccccgt catctccatg
     1501 gcgatcgagc cgaagacgaa gcttgatcag gaaaaaatgg cgaccgcgct gcagcgcctt
     1561 tcagaggaag atccgacctt ctgggtttac acacacgaag acaccggcca gacgatcatc
     1621 gctggcatgg gcgaattgca tctcgaaatc atccgtgacc gcatgttccg cgagttcaaa
     1681 gtcgacgcga acgcgggcaa gccgcagatc gcgtatcgcg aaacgatcac ttcgagtgcg
     1741 cacggcgtcg gcaagttgat caaacaatcg ggcgggcgcg gtcaatacgg ccacgtcgaa
     1801 gtcgacgtgc ggccgaccga gcgcggcaaa ggcctcgtcg tcgagaacaa aatcgtcggc
     1861 ggcatcatcc cgcgtgaata catcccggcg gtgaagaagg gcatcgagga agcggtgctc
     1921 aacggcgtcc tcggcggcta tcccgtcgtc gatgtcgagg tcgacatcgt ttacggctcg
     1981 ttccacgaag tcgactcgag cgagctcgct ttcaaaatgg cggcgatctt cgcgatgaag
     2041 gacgggttca aacaaggcaa accgatcctg ctcgagccga tcatgaaggt cgaaaacatc
     2101 acgcccgatg aatatcaggg cgatatctgt ggcgacttga gccggcgccg cggcagtatc
     2161 caaagcatcg agacacgcgg taacatggcg attattcacg cgctcgtgcc gctcgcggag
     2221 ttgttcggct acgcgacggc tatccgctcg ttatcgaaag ggcgctcgag ctactcgatg
     2281 gagccgtcgc atttcgagcc ggtgccggcg aacctcgtcg ccgcaatcct tgaccagaag
     2341 gagacaaaat gacgaacggc cagcgcattc gcattcgttt gaaagcgttc gatcatcgca
     2401 tgctcgatca atcggcggtc gatatcgtcg agacggcgaa acgcactggc gcgcgcgtgg
     2461 ccgggcccat tccactgccg acgttaattg aaaagtttac tgtgaatcgc tcgccgcacg
     2521 tcgacaaaaa gtcgatggaa caatttgaga tccggacgca caaacggctg ctcgacatca
     2581 tcgacccgac ggcgaaaacg gtggacgaat tgaaaaaatt gaacctgccc gcgggcgtgg
     2641 acatcacgat taagatttag gttttgagaa ttttgcaaga tcgacatcat gagtatcggg
     2701 ttacttggac agaaaatcgg catgaccagc gtctacgacg ccgcgggcaa gctgcacccg
     2761 gtgaccgtga ttgcggcggg cgataacgtt ttgctgcgcc gcctaacgaa tgagaacgac
     2821 ggctactggg ccgtgcaggt cggcttcgat gggcaaaagg aatcgcgcgt gaacaaagcc
     2881 gaactcggcg cgtttaaaaa agccggcgtc gagccgaaga aatttgtgcg cgagtttcga
     2941 ctggatgctg atttgcccga aggggaagtg aatctcagtg tcacgcaatt tcaacctggt
     3001 gatttcgttg atgtgaccgg ccgctcgaaa ggcaaaggct tccaaggcgt aatgaagaag
     3061 cacaatttcc acgggcaagg cgcgtcacac ggttcaaaga cgcatcgccg cagcggcgcg
     3121 attggcaatc gctccacgcc cggccggatt tggaaaaaca tggggatgcc cggtcatctc
     3181 ggtgacgagc gtgtgaccgt gcagaacctc cagatcatgc aggtgcgcga agccgagaaa
     3241 atcattttgg tgagtggcgc ggtccccgga tcgaacggca gttacgtaat cgttaggccg
     3301 gcacttaaga atccggccgt gcgaatcgcg gcacatcagg cgcacgcgaa agaagacaag
     3361 gcagcggcgg cgaagaagcc ggcgataacc gtgcgataag ggatgagcgc gaaagtttta
     3421 agcaaagccg cggcaaaaga agcgaagatc gagattatcg aggatggtcg cggcacacag
     3481 gccgtgcacg atgtcgtggt cgcgatgcgc gcagcgcgtc gttccggctc ggccaacaca
     3541 aaaacaaaag cagaagtgga tttatccggg gcgaaaccgt ggcgtcaaaa aggaaccggt
     3601 cgcgcgcgcg cgggatataa atcgtcgccg atctggcgcg gcggcggcgt ggtctttggc
     3661 ccaaaaccgc gcgattactc caagaaaact tcgaaaagtc tgcggcggct cgcctttcaa
     3721 aaagcgctga gtgagaggat caacgccggc gacgtgttga cgatcgacaa gttcgtcgtg
     3781 ccggagatca aaaccaaatc tttcatctcg ttgttaaaga aagcgactga cgcgggaaaa
     3841 gttttagtga ttgctgattc atttgatgaa aagactttta aatcggcgcg gaatgtgaag
     3901 acagctcacc tggccaccgc ggcggacgta aacacggagc agctgctcgc gtttgagaaa
     3961 attttggtga cgcaagcggc gctgggaaga ctcgcggaac ggacgaaccc accctcacct
     4021 ggcgcttcgc gcttgccgag ccatagctct gcggaggctg gtcctcctct ccctcaggga
     4081 gaggacagag tgagggcaca gccgaaggaa accgcgaaat gatcgaggcg cacgacatca
     4141 ttcagacggc gtcgttgacc gagaagtcga cgctaatgag cgagaagcaa aacaagtacg
     4201 tctttcgcgt ccatccgcgt gcgaacaaga tccagatcaa gcaggcggtg gagcgattgt
     4261 tccaaaagaa ggtggtcgat gttaacacct gcaattacgc cggcaaatgg gcgcgggtgc
     4321 gcggaccgat tgggcgaaag gcgagctgga aaaaagcgat cgtgacattg aaggaaggcg
     4381 agaagatcga cttggtatga ggacgctcag ttcacaaatt atagccgctg tcatcctgag
     4441 ccgtgcagac ggcgaaggac ctcgcaaatc atcaacagcg agttccgtcg aggaagcttt
     4501 gagagatccc tcgacttcgc tcgggatgac aaattgatat ggcactgaaa agttttaggc
     4561 cactcactcc tgttctgcgg ttcaagcagc tgcccgtttt cgatgagatc acgaagtcgc
     4621 gcccggcgaa gagcctgctc gagccaaaaa agaaaagcgg cggacgcaat aattttggca
     4681 ggctcacatc gcgtcacatc ggcggcggac acaagcagca ttatcgcaag gtcgatttca
     4741 aacggcgcaa gcacggcgtc acggccgagg tgatcggcat cgagtacgat ccgaatcgtt
     4801 ccgcgcgcgt tgctcttatc aagtacgctg atggcgagct gagttacatc ctggcgccaa
     4861 atgggctggg cgtgggatcg acagttgccg ccggtcctga cgtcccgccg gatctcggga
     4921 acgcgctgcc gctccgcgcc attccgttag gcacgaacat tcacaacatc gagctgagcc
     4981 cgggccgcgg cgggcagatc gcgcgcagcg cgggacagca ggcaacgttg agcaacctcg
     5041 aaggggatta tgcccttgtg aaaatgcctt ccggcgaagt gcgccggatc cacgcgaact
     5101 gcttcgcgac gatcgggcag gtcggcaacg tcgatcatat gaacgtgtcg gcgggcaagg
     5161 ccggccgttc gcgttggtta ggccgcaggc cgcacgtgcg cggcatggcg atgaatccga
     5221 tcgatcaccc gatgggcggc ggacagggta aatcgaaagg cggcggggga cgacatcacc
     5281 cggtcagtcc gtggggacag ctcgcaaaag gatttaagac gcgcagcaaa cacaagccga
     5341 gcgaccggtt cattttggaa aggagaaggt caaagaagaa agcttagacg tgaatcgtga
     5401 atcgtgaatc gagtgctgct ccgattcacg agtttcgaat cacgaattac ttgcaaatta
     5461 tggctagatc gttgaagaaa ggacccttcg tcgagtcgca tcttctggag aagatcgata
     5521 agatgaacaa cgcgggcgcg aaaaaagcga ttaaaacctg gtcgcggcgc tcgatggtga
     5581 cgccggattt tgtcggccac acgtttttgg tgcacaacgg gaagacgttt cagtccgtgt
     5641 ttgtcaccga gaatatggtc gggcacaagc tcggcgaatt ttcgccgacg cgcgtgttca
     5701 agaaacatgg ttcgcacacg gcgaaggtga ccaagtagtt gagaattttg ttagcgatgt
     5761 tgggctgcgt gacgctggct cgcgggctgg cggcagaggg aaatagttct ttgtcagaat
     5821 ccaaaatcga agacgcggaa cacgcggtca cagccgaggc gttgttcgcg cagaacgaag
     5881 agctgcggcg gcaattgtcg atcgagcagg aatcagtcaa aacattgaca atgagcctgg
     5941 cggaatcgaa tgccgaggcc gaattgtttc ggcgcaagtt ctcggatttg aatctgcgga
     6001 tggaagcgct cggtctcgcc tcggcgagca aagatcgcgc gaagctggag caacgattgc
     6061 tcgcggcggt gagcgatctg cagctcgcac aaaaggaacg cgacgaatat cgcgaccaga
     6121 tgctgcggct gaatgaagcg atgctgcgtt acctgaaatc gtcgcagggc ggcgacgcgc
     6181 aggcgcggat ggacgtagaa gcgcaattgc gtggaacgaa cgcgctggtg acgaaatcga
     6241 cttcagcacg gccggacgcg cagccgagct tgctcgatgg caaggtgatt agcgtgaagg
     6301 acgaatggtc gtttgtcgtg gcgaacttgg gagaaaagca cggggttaag atcggcatgc
     6361 cgctgcgagt tatgcgcggc gagcagacga ttgcgacgct gcgcgtggtg gatgtgcgcc
     6421 agcagatttg cggcgcggtg atcgaggaaa tggattcgaa caaagaaaag ataaaagtgg
     6481 gggatcggct tcaggtcgat gccaaaccga gtgtgagtgt cagatgaaca agggttgaag
     6541 cgatgaagag ttgaagggtt gaagcggaga gaatgaaagc cgctttaacg ctttaaccgt
     6601 ttaacgctta acgcaaagta aatggaagtt agatcgatct acaggtatgc gcgaatctcg
     6661 cccttcaagg tgcgggaagt gacgcgcgag attcaggggc tgccggtctc ggccgcgctc
     6721 gacgtgctcg cattcacccc gaagaaggcg gcgttcctga tcaacaagac gctgaagagc
     6781 gcaatcgcga acgcggagaa caacgcgcag ctcaaagcgg acggtctcgt cgtcaaagaa
     6841 gcggtcgtcg gcgaggggcc aaccttcaag cgaatcatgg cgcgcgcgcg cggaagcgcg
     6901 agccggattt taaaacgcac gagtcacatt cgcatcgtgc tctcggacga gatcccgatc
     6961 gagacacgcg agacgcgaaa agcgaagcgc aaagcggaag agaaaggcgc caagaaagac
     7021 ggcgaagccg gcgagacgaa ggaaaaatcg acaactgctg caaagaaaaa gccgaaggcg
     7081 aagaagcagc cagcgagaaa aggataatat atgggacaaa aagttaatcc gattggattt
     7141 cgactcggtg tgaaccgcga ctggcgctcg cgctggtacg cgtctccgca agagctaccg
     7201 gcgtttttgc acagcgacct cgagatccgc cattacgtaa agaaaaagct gcagttcgct
     7261 gcggtctcga aaatcgtgat cgagcgcgcc tggaactcca ttcgcgtgac cattttcaca
     7321 gcgcgtcccg gcatcgtgat cggccgcaag ggcgccgaga tcgagaagat gacggaagag
     7381 attgcgaaga tggcgcaagg caaacagatc aagattgaca tccaggagat caagacgccc
     7441 gagctcgacg cgcagttggt cgcggagaac gtcgcgttgc aaatcgagcg ccgcatcgct
     7501 taccgccggg cgatgaagaa ggctgtacag acggcgatgg attttggcgc gcagggaatc
     7561 cggttgcgtt gctcgggtcg attaaacggc gcggaaatct cgcgctcgga atggtatcgc
     7621 gaaggcaaag tgccgctgca cactttgcgg acgggaatcg attacggttt tgccgaggcg
     7681 aacacggttt acggcaagat cggcgtgaaa tgctggatgt gtaagaagga agaagcgccg
     7741 gccgaagcgc cgacaccacc agcgccacct ccaccgccgg agccgcagcc cacggaagtt
     7801 taaggagaat tgttatgccg ttgatgggac tgaattctgt agccgcgtcc ctgtgggacg
     7861 cgtgcgcgct tcgcacagcg aagcggctac agcatcaaac gatcagatgt aacggagcag
     7921 aattgttatg ccgttgatgc cgaaacgtgt gaagtatcgc aagtcccagc ggggaagccg
     7981 caaggggacc gcttcgcgca acctcagaat cgactttg
//
LOCUS       DASTDG010000065         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_579951, whole genome shotgun sequence.
ACCESSION   DASTDG010000065 DASTDG010000000
VERSION     DASTDG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579951"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1095)
                     /gene="gap"
                     /locus_tag="VFO30_02000"
     CDS             complement(94..1095)
                     /gene="gap"
                     /locus_tag="VFO30_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9418087.1"
                     /translation="MANYSINGFGRIGRNVLRAMTQKQVERVRAINDLTDTKTLAHLL
                     KWDSVHGKFDGEIGYDEENIIVRDHAIRIFKERDPGNLPWKKLEVDVVLESTGFFTAR
                     DKADLHISKAGAKRVLISAPAKNPDATLCIGINDDIYNPDKHTVVSNASCTTNCLAPL
                     AKVLNDNFGVAHGFMSTIHSYTNDQRILDFPHEDLRRARAAAISIIPSSTGAAKAIGE
                     VIPELKGKLNGGAFRVPTPDGSVTDFSAVLERDATIDAINAAFKEAAADKSYKGVLEY
                     TDEPLVSRDIVGNPHSCIFDSKLTLMLGNRFVKVVGWYDNEWGYSNRCVEMMELLGK"
     gene            1175..1870
                     /locus_tag="VFO30_02005"
     CDS             1175..1870
                     /locus_tag="VFO30_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET9418088.1"
                     /translation="MGTRAKQLRTVGALAVALSILCTPTIRGARDDSSPMKTIFVFGD
                     SLSDGFHLKRSQAYPTLLLGKLRDVGLEYEIVSASQSGGTTTSGLARLPPHLKRKIDI
                     FILELGINDAFLGVPVDTIEANLQSIIDQVKARNPQVRIVICGMQVRNFSADAYVAAF
                     GKMYSELAEKNRAALVPYLLDGVGGDPNLNLPDGIHPNSAGQKILAENVWRVLEPIAR
                     EVAAASSQAATAN"
     gene            2124..2414
                     /locus_tag="VFO30_02010"
     CDS             2124..2414
                     /locus_tag="VFO30_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9418089.1"
                     /translation="MDPARPGELEPQDPFNPERADHAILETIEIATEGEDCDECVRRL
                     RPPLMKINGVKDVIVDLERERVVVTFDARKTHAPDLHDAILQTGYKPAAEAE"
     gene            complement(2565..3593)
                     /locus_tag="VFO30_02015"
     CDS             complement(2565..3593)
                     /locus_tag="VFO30_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET9418090.1"
                     /translation="MAPTTETPAAQGYRMPAEWERHEATWLSWPRREGISFPGSFDRV
                     MPTLRAMVEALVESERVCINVCNGAHEAEAQEVLDGLPMQRITFHRISTNEPWCRDHG
                     PIFLTRDADPKLAVVDWDYNAWGGKYPPCDLDEVVPTRVAESLKLPVFYPRMILEGGS
                     IDVNGAGMLLTTESCLLNKNRNPNLSRDEIEQRLRDYLGAREILWLGRGIAGDDTDGH
                     IDDIARFVSETKVVAVVEEDRDDENYEPLQENLSRLSGFGLDIVTLPMPRKIVREGLR
                     LPASYANFYIANSCVLRPTFADPSDETALSILQQCFPTRRVIGIDCRELIWGLGTFHC
                     LTQQQPTI"
     gene            complement(3669..4187)
                     /locus_tag="VFO30_02020"
     CDS             complement(3669..4187)
                     /locus_tag="VFO30_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418091.1"
                     /translation="MQNEGFLLGIAQIAVTLAGFSGLVVAIRGAPPTAWHPRDIWSLS
                     WMFGTSLGALFMALLPLLLAFFHLEAGLLWTIASLAMSAFMIVFSVAMALAGRRLTRL
                     GHRPRVRFFPMAATFLLLICGFLSGLGGAGLFPQARIGFFILGLIACLFVSALSLVVF
                     LVVLARAAGSRL"
     gene            complement(4320..4859)
                     /locus_tag="VFO30_02025"
     CDS             complement(4320..4859)
                     /locus_tag="VFO30_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418092.1"
                     /translation="MKPPETFRTARLVLRKPTREDAALMFAAYGQDHEVAHYVTWRPH
                     TCVSDSEAVIGRFLDLWRDGSGFHWLLFQYSGSELMGAIGVRREAHRLELGYVLARRY
                     WGYGFMAEAVTAVVDWAFTEPSVFRVGAVCDIDNQRSARLLERAGFEREGVLRSWAVH
                     PNISATPRDCYSYSKFRNI"
     gene            complement(4934..>5828)
                     /locus_tag="VFO30_02030"
     CDS             complement(4934..>5828)
                     /locus_tag="VFO30_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET9418093.1"
                     /translation="GVDLAVVARGTPGYSGAELANVINEAALLAARRGLKGITLAELE
                     EARDKVRWGKERRSLALSEKEKQNTAYHEAGHALLLELLPNTEPLHKVTIIPRGPSLG
                     STMWLPEEDKYTNRKNELIASLVVKMGGRVAEEIIFGDVTNGAAGDIKMATAMARKMV
                     CEWGMSEKLGMVEYGEHEDYVFLGRDISRARDYSEATAEEIDHEVRRLLDEAYQAAKQ
                     TLLAHRDKLEVIAKALLEYETLDGSQIKEIVEHGRLLNPPPAVTPTSGQKMPPEKPPK
                     QVVVGPEVAPPLPGALGGAPA"
BASE COUNT         1211 a         1754 c         1652 g         1211 t
ORIGIN      
        1 caattgcttc ccgataaaca tcaagccgct cgtgatcgaa gtacgtcttc atagttcgag
       61 catgagcagg agcacgagca tgagcaagac gccctatttt cccaacaact ccatcatttc
      121 gacgcagcgg ttgctgtagc cccactcgtt gtcgtaccag ccgactactt tcacgaaacg
      181 gttgccgagc atgagagtga gtttgctgtc gaatatgcat gagtgcggat tgcccacgat
      241 gtcccgggac acgagcggct cgtcggtata ttcgagcacg cctttgtagc ttttgtccgc
      301 cgccgcttcc ttgaacgcgg cattgattgc gtcgatcgtc gcgtcccttt ccaacaccgc
      361 gctgaaatcg gtgaccgagc cgtccggggt tgggacgcga aacgcgccgc cgtttagttt
      421 tcctttcagc tccggaatca cttcgccaat cgccttggcc gcgcccgtgc tcgacggaat
      481 gatgctgatc gccgctgccc gcgcacgccg caagtcttca tgcgggaaat cgagaatccg
      541 ctggtcgttc gtgtagctgt ggatcgtgct catgaacccg tgcgccacgc cgaagttgtc
      601 gttcaagacc ttggccagcg gcgcgaggca attggtcgtg cagctcgcgt ttgaaacgac
      661 ggtgtgctta tccggattgt aaatatcgtc attgatcccg atgcagagcg tcgcgtcagg
      721 attcttggcc ggcgcggaaa tcaaaacccg tttcgcgccc gctttggaaa tgtgcaggtc
      781 ggctttgtcc cgcgccgtga aaaagccggt cgattccagc acaacatcga cctccagttt
      841 tttccacggc agattgccgg ggtcgcgctc cttgaaaatg cggatcgcgt ggtcgcgcac
      901 gatgatgttc tcctcgtcgt aaccgatctc gccgtcgaat ttcccgtgca ccgagtccca
      961 tttcagcagg tgcgcgagcg ttttcgtatc ggtcaaatcg ttgatcgcgc ggacgcgctc
     1021 gacttgtttt tgcgtcatcg cccggagcac gttgcgcccg atgcggccga agccgttgat
     1081 agagtagtta gccataggta gaatttgcga tttgcgagtt ccgattttcg attgccgagc
     1141 aaatcttcgg caacgaacga ggcgggaaat taaaatgggc acacgcgcga agcaattgcg
     1201 aactgtcggc gcgctcgccg ttgcattgtc gatcttgtgc acgccaacca ttcgcggcgc
     1261 gcgcgacgat tcttcaccaa tgaaaacgat cttcgttttc ggcgacagtc tgtccgacgg
     1321 ctttcacctc aaacgcagcc aggcatatcc gacgctgctc ctgggcaagt tgcgcgatgt
     1381 cggcttggaa tacgaaatcg tcagcgccag ccaaagcggc ggcaccacga cgagcggcct
     1441 cgcgcggctc ccgccgcatc tcaaacgcaa gatcgacatc ttcattctcg agctcggtat
     1501 caacgacgcc tttctcggcg taccggtcga cacgattgaa gcgaatcttc aatcgattat
     1561 cgatcaggtg aaagcgcgca atccgcaggt gcgaatcgtc atctgcggca tgcaagttcg
     1621 taacttctcc gccgacgctt acgtcgccgc gttcggcaaa atgtattccg agctggcgga
     1681 gaaaaatcgc gccgcccttg tgccttatct gctcgacggc gtcggcggcg atccgaattt
     1741 gaacttgccg gacggcatcc atccaaacag cgccggccaa aaaatcctgg ccgaaaacgt
     1801 ctggcgcgtt ctcgaaccaa tcgcgcgcga agtagctgcc gcgtcgtcgc aagccgcgac
     1861 agcgaattaa aagaaaacgt cgaacgctga acgctcaaca tccaattctc gaatgcagag
     1921 ctccgccttc gacattcggc gttggacgtt cggcatttgc ttctgctggg gttgtagcca
     1981 cggccctgcg ggccgtctgc tcccgcacgt gttttaccct caccggcgct gatggtgttc
     2041 gcctctccct cagggagagg attgaggtga gggccagtgc ttgagatgca gccagcgggt
     2101 tcgtgtgcgc cgtagaataa gtgatggacc cagcgcgacc aggcgagctt gagccgcagg
     2161 acccgttcaa tcccgagcgc gccgatcacg cgattctcga aacaatcgag atcgcgaccg
     2221 aaggcgaaga ttgcgacgaa tgcgtccggc gcttgcgccc gccgctgatg aagatcaatg
     2281 gcgtgaaaga cgtgattgtc gatcttgagc gcgaacgcgt cgtcgtcacg ttcgacgccc
     2341 gcaaaacgca cgcgcccgat ttgcacgacg ccattctgca aaccggctac aaaccggcgg
     2401 ctgaagcgga ataagcaaac gccgaacgcc caacgtccaa tctcgaatgc agagagccgc
     2461 agtctgattt cgatattcgg cgttcggcgt ttttcttctg ctaacttgta gccacggccc
     2521 tgcgggccgt ctgctaggat gtgccataga cacgtggcta cagatcaaat cgtcggttgt
     2581 tgctgcgtca ggcaatgaaa cgtgccgagg ccccagatta attcgcggca atcgatcccg
     2641 atgacgcgac gcgttggaaa acattgttgc aagatcgaca atgcggtttc gtctgacgga
     2701 tcggcgaaag tcgggcgaag cacgcaactg tttgcgatgt agaagttcgc atagctcgcg
     2761 ggcaatcgca gaccttcgcg aacgatcttc ctcggcatgg gcaacgtgac tatgtccagt
     2821 ccgaagccgg acagtcggct gaggttctct tgtagtggct cgtagttttc gtcgtcgcga
     2881 tcttcttcaa cgacagcgac aacttttgtc tccgaaacga accgcgcgat gtcgtcgatg
     2941 tgaccatcgg tatcatcgcc ggcgatgccg cggccgagcc agagaatttc gcgcgcgccg
     3001 agataatcgc gcaggcgctg ctcgatttca tcgcggctca ggttcggatt gcggttcttg
     3061 ttcaacagac aggactcagt cgtgaggagc atgccggcgc cgttcacgtc gatcgagccg
     3121 ccttccagaa tcatgcgcgg ataaaaaact ggcagcttca gtgattcggc cacgcgcgtc
     3181 ggcacgacct catccagatc gcacggcgga tatttgccgc cccaggcatt ataatcccaa
     3241 tccacgaccg cgagttttgg atccgcgtca cgcgttagaa aaatcggtcc atgatcgcgg
     3301 caccacggct cattagtcga gattcgatga aacgtgattc gttgcatcgg caaaccgtcg
     3361 agaacctctt gcgcctcggc ttcatgcgcg ccattgcaaa cgttgatgca gacccgctcg
     3421 gattcgacga gcgcctccac catcgcgcgc agcgtcggca ttacccgatc gaacgacccg
     3481 ggaaaactga tcccttcccg ccgcggccac gacagccacg tcgcctcatg ccgctcccat
     3541 tccgccggca tccggtaacc ctgcgcggcg ggcgtttccg tcgttggagc cattcggagc
     3601 aacgtgtgac gcgaaatgcg ccgcgtccaa tgggccgagc cgttttctac ttccgacttg
     3661 ttactcgttc acaaccgcga tcctgcggcg cgcgccagca ccactagaaa cacgacaagc
     3721 gagagcgctg acacgaacaa gcatgcgatg aggcccaaaa tgaagaagcc gatcctcgct
     3781 tgtgggaaca gaccagcgcc tcctaggccc gataaaaaac cacagatcaa aagtagaaat
     3841 gtcgccgcca tcggaaagaa gcggacacgc ggccgatgac ccagtcttgt aagacgtcgc
     3901 cccgccaggg ccattgccac actgaagacg atcataaaag cagacattgc cagactggcg
     3961 attgtccaaa gcagtccagc ttctaagtga aagaaagcta ataacagtgg cagcaacgcc
     4021 atgaaaaggg cgccgagact cgtcccgaac atccatgaca acgaccagat gtcccgggga
     4081 tgccaggcgg tcggaggcgc accacgaatg gccactacca agccggagaa tcctgcgaga
     4141 gtcactgcga tctgcgcgat ccccaagaga aacccttcgt tttgcatggg aatctctgtc
     4201 gcctaacgag acaaagctca gccgccgctg gcgagagcga gccttgcttt cacttcatcc
     4261 ttcataattc atccttcaac cttttcccgc gccggtcagc ggttggctgc agcgcctggt
     4321 tagatgttgc ggaatttgga atacgagtag cagtcgcgtg gcgtcgctga aatgttcggg
     4381 tggaccgccc acgagcgtaa aacgccctcc cgctcgaagc cagctctttc caggaggcgc
     4441 gccgagcgct gattgtcgat atcgcagaca gcgccgacgc ggaaaacaga cggctcggta
     4501 aacgcccaat cgaccaccgc ggtcaccgct tcggccataa atccgtagcc ccaatatcgc
     4561 cgcgctagga catatccaag ctcaagccgg tgtgcttctc tgcggacacc aatagcaccc
     4621 atgagctcac tgccgctgta ttggaacaac agccagtgga acccggatcc gtcgcgccac
     4681 aaatcaagaa agcggccaat gacggcctcg ctgtcggaaa cgcacgtatg tggacgccac
     4741 gtgacgtagt gcgcaacctc atggtcttgc ccgtaggccg cgaacatcag agccgcatcc
     4801 tcacgcgtgg gcttccgcag aaccaatcgt gccgtacgaa acgtttccgg tggtttcatt
     4861 acccgatgcg cgcgagtaac atctaacgag aattagacga aaagtttcgt ctaacaagtc
     4921 gtggcgtcag ccgtcaggcg ggtgcgccgc ctaatgcgcc ggggaggggc ggggcgacct
     4981 cggggccgac gacgacttgc ttgggcggtt tttcgggcgg cattttttgg ccggacgtcg
     5041 gcgtgacagc gggcggcgga ttgagcaggc ggccgtgctc gacgatctcc ttgatctgcg
     5101 agccgtcgag cgtttcgtat tcgaggagcg ctttggcaat gacttcgagt ttgtcgcggt
     5161 gcgcgagcaa agtttgtttg gcggcctgat acgcttcgtc gagtaaccgg cgcacttcgt
     5221 ggtcaatctc ttcagcggtg gcttcgctgt aatcgcgcgc ccgggagatg tcgcgcccga
     5281 ggaaaacata atcctcgtgc tcgccgtact cgaccatgcc gagcttttcg ctcattcccc
     5341 attcgcaaac catcttgcgg gccatggccg tggccatctt gatgtcgcca gcggcgccgt
     5401 tggtgacgtc gccgaaaatg atttcctcag cgacgcgccc gcccattttc acaaccagac
     5461 tggcaatcaa ttcgtttttg cgattggtgt atttgtcttc ctccggcaac cacatggtcg
     5521 atccaagcga cgggccccgc gggatgatcg tgaccttgtg gagcggctcg gtattgggca
     5581 aaagctcgag caacaacgcg tgaccggcct cgtggtaagc ggtgttttgt ttttcttttt
     5641 cgctcagggc gaggctgcgg cgttccttgc cccagcggac tttgtcgcgc gcttcttcca
     5701 attcggcgag cgtgattcct tttaatccgc gacgcgcggc gagcagcgct gcttcgttga
     5761 tgacgttggc cagctcggcc ccggaataac cgggcgtgcc gcgcgcgacc acggcaagat
     5821 cgacacct
//
LOCUS       DASTDG010000066        11181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1019274, whole genome shotgun sequence.
ACCESSION   DASTDG010000066 DASTDG010000000
VERSION     DASTDG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019274"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFO30_02035"
     CDS             complement(<1..239)
                     /locus_tag="VFO30_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418094.1"
                     /translation="MKIYISGLHSGPNPQPGVGVTRSRRHDYSRARFRPATFLRLFLS
                     PHFCEARRAMLAKIYSAALYGVDAYEVEVEVNGAHG"
     gene            631..1485
                     /locus_tag="VFO30_02040"
     CDS             631..1485
                     /locus_tag="VFO30_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9418095.1"
                     /translation="MAAEDSDTGIKIYLREIGQIPLLTPQQEIELAAKIKKGDREARA
                     LMIRSNLRLVVKIAHDYANLGLPLLDLISEGNIGLMKAVERFDPAKGGKLSTYAAWWI
                     KQSIKRALANQSKTIRLPVHLVDKISKMRRVSLQMSEELGREPTDDELGEEIGIAAGK
                     VSQLKTVSIRPASLDAPISDDDSTEFGEIVGDEEAQTPFELLRDKNLRNEVGGLLDVL
                     DDREKKIIFQRFGLDGGKPKTLEEVGKKFGVTRERIRQLQNIALSKLRRALSKKEKPV
                     DVELPVEA"
     gene            complement(1545..2948)
                     /gene="pyk"
                     /locus_tag="VFO30_02045"
     CDS             complement(1545..2948)
                     /gene="pyk"
                     /locus_tag="VFO30_02045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9418096.1"
                     /translation="MRRTKIICTLGPASTRAEVLRRLIDQGADVFRLNMSHATHDWVR
                     EIVPRVQTLARKAGRTIALLLDTQGPAIRTGDVKANLELKPRDVLEFTTRDAKPKERY
                     SVEVNYRDFARDVAPGQTVLVDNGMIKLLVLETKRNRVRCRVLTRATLGSRRHINLPG
                     VYVNLPPLTKKDIVDVALGAELGVDFVALSFVREKSDIEQLRKLLAKNKSKALVVAKI
                     EDQLAVRALDKMIESADAIMVARGDLGIECPMEELPVIQRRIVKDCIRLGKPVIVATQ
                     LLESMITNPLPTRAEITDVANAVFEQADALMLSGETSIGRYPADCVEVLDRVARRIER
                     SGDAGYAQFAVLEDLRQKMVASAVVLANSLYRAKIIVFTRRGTMARNVSNLRPARAPI
                     FAFTPNEQVQRRLSMFWGISPVLLNFAADPNATIEAAEKYLRENKLTTAGDQLIIISD
                     VPAGASFADAVQLRTAK"
     gene            complement(3058..4467)
                     /locus_tag="VFO30_02050"
     CDS             complement(3058..4467)
                     /locus_tag="VFO30_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418097.1"
                     /translation="MAVKTESELSETQRAHWLKAVAAIELRNFGYAISLLQGILKQEP
                     EFLTGRRLLRRAEVAKTRAAKKSVFNISTAPIAVMKAQREVRKDPKRAVEMIEKVLEE
                     QPYHRQANLLLKEAAVSAGWPEIGIFALRTLLEENPRDLKVLHELGRLYHALSQSEEE
                     VEVYNRITEIDPLDAEALRLGKDASARASMASGGWTQAASYRDLIKDKDLAVSLEQQS
                     RMKLSNESLDQQIADTYGTHQAHPENVDLARRLGVLHEQKNDLESAITWYQYAADLTK
                     GSDAGLVRKLSDLKIKQLDRQIVQQEESLLMAVGENELGANCSEELRHLKQQRAELLI
                     GDARKRVERNPTDLQLRFELGEHLVNAGCYREAVPELQRARQNPNSRLKAMNLLGRSY
                     AELGMLDLAARQLADAAKEIFTMDATKKEIVYNLGLVYERMGERDKSLECMKQIYESD
                     YGYKDVAARVESSYEQKLS"
     gene            complement(4886..7393)
                     /gene="mutS"
                     /locus_tag="VFO30_02055"
     CDS             complement(4886..7393)
                     /gene="mutS"
                     /locus_tag="VFO30_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9418098.1"
                     /translation="MQDALTPMMQQYRRLRGSIPADTLLLFRLGDFYELFFEDAKEAS
                     ALLNVALTKRNSVPMCGVPYHAAQSYIAKLIKAGKRVAICDQTSEPQSGRIVDRDITQ
                     IISAGTVSDLDLLESKRANYLGAVYIDNRVYGFAYADLTTGEFRLAQLPDRQSLLDEL
                     ARVAPTELLIPEEQKEQLAGFGRAISYDSYAFLPEHASFTLCEHFKVKSLDGFGCTQM
                     SAAVAAAGALVHYLKHQLRRKIDHLSSLKCDAASEHVIIDAATQTNLELVESRSARDV
                     SLLAVLDRTVTPMGGRKLRSWILQPLRNLKELEWRQRMIADLLREPDVLAALRAQLKA
                     IRDIERAVGRLSQASGNARDLLALKTSLQQIPQLKRHVQALLDRAAFGTNRARETDEV
                     TSLAQHLQNEIEEMPELAEKLARALVDEPPFQLKEGGIFRDRYDVDLDELRRASREGK
                     DWISQLQEREIAASGIKSLKVRYNSVFGYFIEVTKANLGSVPPHYTRKQTTVGGERFV
                     TPELKEMEAKILGADERARNLEYELFQKLREEALRELGAIQQTADAIAIVDVICSFAE
                     TARLFNYCRPVLNDSLRLCIKDGRHPVLDQNLVEEKFVPNDTELDEKIRMLIVTGPNM
                     AGKSTYIRQVALITLMAQIGSFVPAASAEVGLVDRIFTRVGANDDLARGQSTFMIEMN
                     ETANIVNNATARSLVILDEIGRGTSTFDGLSIAWSVAEFLHDKIKARTLFATHYHELT
                     KLAAERTGAANFNVAVREWNDQIIFLRKIVPGGADKSYGIQVARLAGLPKEILDRAKD
                     ILAHLENPNGATAQPKPRRKRSTSTRTEPNKPQLDLL"
     gene            complement(7597..8616)
                     /locus_tag="VFO30_02060"
     CDS             complement(7597..8616)
                     /locus_tag="VFO30_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9418099.1"
                     /translation="MKRKLKVLVLCDGVRPTKIDEDLSQEMKTEDWKTEANVIAALGE
                     RGYTAEQLAIFDDVDLVRQKLETFPADLLFNLVEQFKNNPGFDQNIVSLLELQDVPFT
                     GCGATGLTLCKDKGISKKIVGYHGIGVPNFLVIPRGQRNVRPKQLKFPILVKPVKDEA
                     SYGISQASFVQNEEQFRERVAFVHEKHNSDAIAEEYIEGRELYVSIIGNTRLQVFPIR
                     ELVFREVPPNEPKIATYKAKWNEEYRKRWGLENHFADDLEPALAREIADTCKRIYRLL
                     TIDGYARIDLRLAPDNKIYFIEANPNPHLAADEDFALSAGNAGLAYPQLIERIIRLGI
                     KTARG"
     gene            complement(8622..9629)
                     /locus_tag="VFO30_02065"
     CDS             complement(8622..9629)
                     /locus_tag="VFO30_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418100.1"
                     /translation="MDVADWAALSDEELLERRISTLGLRLEGTALEPLIKQLYDDLSA
                     KGLAFHPPSHIGDEWFVPIGIPAIFVPFFLVHERLRALERTMMLEVEGETPEWFMRLM
                     RHEAGHAYSYAYQLQRKKKWQNCFGHTSGESTPTTYRPRPFSRSYVVHLEDWYAQAHP
                     DEDFAETFAVWLTPGLDWRERYTGWPARRKLEYVDGLMHSLAGKPPVHAPKYRVADYN
                     CLNVKLKTYYARKRKLYEDIYPGFYDVDLRQLFPAPQGIKASSYLRQRRRRLMNSVCQ
                     WTNEKKYRVNKLLSRLIDRSDELGLHAHNDDPQQDFRVASYITTLVMNYLFTGKFKRT
                     K"
     gene            10879..>11181
                     /locus_tag="VFO30_02070"
     CDS             10879..>11181
                     /locus_tag="VFO30_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418101.1"
                     /translation="MDVPRTGVAAQKRKRRVIMIAASALALVTATFALSRLKTAVPSV
                     ERSAVWIDTVKRGSMLRQVRGLGALVPEEMRWIPANTEGRVEKILVWPGTTVEPDTV"
BASE COUNT         2253 a         3327 c         3112 g         2489 t
ORIGIN      
        1 ccgtgcgcgc cgttcacttc cacttcgact tcgtaagcat cgacgccgta aagcgcggcc
       61 gagtagatct tcgccagcat cgcccgccga gcttcgcaaa aatgcggcga gaggaaaagg
      121 cgcaaaaacg tcgccggcct gaatcgggcg cgtgaataat cgtgccgccg cgagcgcgtc
      181 actcccacgc ccggttgcgg attgggcccg gagtgcagac cgctaatgta aattttcata
      241 ttccatcagc agtcgactta aaacgattgc caactgcgtg tcgcattgac tgtcgcagtc
      301 aaatcgctgc cgactggaat gcaggttgtg cctattttaa aaaatcgacg tagcgccaaa
      361 gcactcgttc gcgcgccaac ctcatcttgc tccggtttgc acacgcaaga aatgcaattg
      421 ccgcgccgcc cgacgccttg tcgccgaggt cggacttgtc gcttgaaaaa gtttgaacga
      481 cgcgcatcca cgcaatgtca gagatgcgaa atatttaacc ttgttaacta cttggtcgga
      541 tgctaactag tttggttcac gaggccctga caccccccgg ctgccgcggc tcgtcgatta
      601 aaccccccac cacagtgcat gaaaagctat atggctgctg aagatagcga taccggaatt
      661 aagatttacc tgcgcgagat cgggcagatc ccgctcttaa ccccgcaaca ggagatcgag
      721 ctcgccgcca agatcaagaa aggcgatcgc gaggcgcgcg cgctcatgat ccgttcaaac
      781 ttgcgcttag ttgtcaagat tgcgcatgac tacgccaatc tcgggctgcc gctgctggat
      841 ttgatttccg aaggcaacat cggcctgatg aaagcggtcg agcgcttcga cccggccaaa
      901 ggcggcaagc tcagcactta cgcagcctgg tggatcaaac aatcgatcaa gcgggcgctg
      961 gcgaaccagt cgaagacgat tcgtctgccg gtgcatctcg tggacaaaat ctcgaagatg
     1021 cgacgggtct ccctgcagat gagcgaagag ctcgggcggg aaccgactga cgatgagctc
     1081 ggcgaggaga tcggtattgc tgccggcaaa gtttctcagt tgaaaaccgt gtcgatccgg
     1141 ccagcatcgc tcgacgcacc gatcagcgac gatgattcga ccgaatttgg tgagattgtc
     1201 ggcgatgaag aggcacagac gccgttcgaa ttattgcgcg ataaaaatct ccgcaatgaa
     1261 gtcggcggtt tgctcgatgt gctcgacgat cgcgagaaga aaatcatttt ccagcgcttc
     1321 gggctcgatg gcggcaaacc gaagacactc gaggaagtcg gtaaaaaatt cggcgtgacc
     1381 cgcgagcgca ttcgccagtt gcaaaacatc gcgctatcga aactgcgtcg cgcgctgagt
     1441 aagaaagaaa agccagtcga cgtcgagctg ccggtcgaag cgtaatgtgt agccacgggc
     1501 cctgcgggcc gtcgattcaa cggctcatag agccgtggct acaattactt cgccgtgcgc
     1561 aattgcaccg catcagcaaa cgacgctccg gcaggcacgt cgctgatgat gatcaactga
     1621 tcgccagccg tagtaagttt gttttcccgc agatatttct ccgctgcttc gatcgtcgca
     1681 ttcggatctg ctgcgaaatt cagcaacacg ggcgagatgc cccagaacat cgacaatcgc
     1741 cgctgcactt gctcgttagg tgtgaaagcg aagatcggcg cgcgtgctgg gcgtaagttc
     1801 gagacgttgc gggccattgt tccccgccgc gtgaagacaa tgattttcgc gcggtacaac
     1861 gaattcgcca ggacaaccgc cgaagcgacc attttttgcc ggagatcctc cagcacggcg
     1921 aactgcgcgt agccggcatc gccgctgcgc tcaattcgcc gcgcaacgcg atccaatacc
     1981 tctacgcaat cggccgggta gcggccaatg ctcgtttcac cgctcaacat caacgcgtcg
     2041 gcctgctcga acactgcgtt ggccacgtcg gtaatctcag cgcgcgtcgg caatggattc
     2101 gtgatcattg attcgaggag ctgggtcgcg acaatcaccg gtttgcccaa gcggatgcag
     2161 tccttcacaa tccggcgctg gattaccggt agctcttcca ttgggcattc aatgcccaga
     2221 tcgccgcggg ccaccatgat cgcatcggcg ctttcgatca tcttgtccaa agcgcgcacc
     2281 gcgagttggt cttcaatctt ggcgacgaca agcgctttgc tcttattctt cgccagcagc
     2341 ttgcgcagtt gctcaatgtc gctcttctcg cgaacaaagc tcagtgccac aaagtcgact
     2401 ccgagttccg cgccgagcgc aacatcgaca atgtcttttt tcgtgagcgg cggcaggttg
     2461 acgtaaacac cgggcaagtt gatatggcgc cgcgagccga gcgtcgcgcg cgtgagaact
     2521 ctgcagcgca cccggttcct tttcgtctcg agcacgagca gcttgatcat gccgttgtct
     2581 accagcaccg tctggcccgg cgcgacatct ctcgcgaaat cacggtagtt cacctcgacc
     2641 gagtagcgtt ccttcggttt cgcgtcgcgg gtagtgaatt cgagaacgtc ccgcggcttc
     2701 agttcgagat tggctttcac gtcgccggtg cggatggcgg gaccttgcgt gtcgagcaaa
     2761 agcgcgatgg tgcggccagc tttgcgcgct agcgtttgca cgcgcgggac gatttcacgc
     2821 acccaatcgt gcgtcgcatg actcatgttc agccgaaaca cgtccgcccc ctggtcgatc
     2881 aaccggcgca acacctcagc acgcgtgctg gccgggccaa gcgtgcaaat gatcttcgtc
     2941 cttcgcatgg tggatcggct tctccgaagg cgatgttaga atttgagcgg cttcgccgcc
     3001 attattaaca tcgcgctcgg agatcgcgat ccacctctgc gtcagcgcgc gattgcctca
     3061 ggaaagtttc tgctcgtatg agctttccac ccgcgccgcc acgtccttat agccgtagtc
     3121 ggactcgtaa atctgcttca tgcattcgag cgatttgtcg cgctcgccca tccgctcgta
     3181 gaccagaccc aagttgtaca cgatctcctt ctttgttgcg tccatggtga agatttcctt
     3241 cgccgcatcc gccaattggc gcgccgccag atcgagcatg ccaagctcgg cataggagcg
     3301 gccgagcaag ttcattgctt tcagccggga atttgggttt tgccgcgcgc gttgtaactc
     3361 gggaaccgcc tcgcgatagc aaccggcgtt gaccaggtgc tcgcccaact caaagcgcag
     3421 ttgcagatca gttgggttcc gctccacccg cttccgcgcg tcaccgatga gaagttcggc
     3481 gcgctgctgc tttagatggc gcagttcttc ggagcagttt gcaccgagct cattttcgcc
     3541 aaccgccatt aagagagatt cctcctgctg cacaatctgg cgatcgagct gtttgatctt
     3601 cagatcggaa agtttgcgca ccagtcccgc gtcgctccct ttcgtcaggt cagctgcgta
     3661 ctgataccaa gtaatggccg actcgagatc gttcttttgc tcatgcagga cgccgagccg
     3721 ccgcgcaaga tcgacatttt ccgggtgcgc ttgatgggtg ccgtaagtgt cggcaatctg
     3781 ctggtcaagc gattcgttgc tcaacttcat ccggctctgc tgctcaagcg aaacagctag
     3841 gtccttgtcc ttgatcaagt cgcgatagct ggccgcttgc gtccaaccgc cgctcgccat
     3901 tgatgcccgg gcggatgcat ccttgcccag ccgcagcgct tccgcatcga gtgggtcgat
     3961 ttcagtgatc cggttgtaaa cctcgacttc ttcttcgctc tggctaagcg cgtggtacaa
     4021 gcgcccgagt tcatgcagca ctttcagatc gcgcgggttc tcctccagta acgtgcgcag
     4081 cgcgaagatg ccaatctccg gccaacctgc gctgaccgcc gcttccttta gtaacaggtt
     4141 cgcctgacga tgatatggct gttcctccag caccttctcg atcatctcca ccgcccgctt
     4201 cgggtctttc ctgacctcgc gctgcgcttt cattacggca atcggcgcag tcgagatgtt
     4261 gaacacgctt ttttttgccg ccctggtttt cgccacctcg gcacggcgta acaaccttcg
     4321 gccggtgaga aactccggct cctgtttcag aatgccctgc agcaacgaaa tagcatagcc
     4381 gaagttgcgt aattcaattg ccgccactgc tttgagccaa tgggcgcgct gtgtttcgct
     4441 cagctcgctc tccgtcttaa ctgccatttc gcagttacaa tatacttcgt tcgttaaccc
     4501 gcgtcagccg aatttctaga agcgacaaag agctaggcat ggacagatta agctagcttg
     4561 ctcctctgtt tcggtgacca agttcgctct gaacgtctgt cttgaacctg atcatttccc
     4621 gaaggtagca tctgaacagg cgcccaattt ttcgggacaa ataacctaac cgggccgcac
     4681 tttgcgtctg ggtccttgcg cggcgtcgcc tcgccccgcg caccgtgctc gttaggcatc
     4741 tgggcacgcc tggtctgctc gacacaggca agagattatg cgttggctcg ccgagaattt
     4801 ggcgcttcga tatgcactgg cgcaacgtga tccccacggc gctccagcct ggctgccgcg
     4861 gatcccacag attcgcggcc gccagctaaa gcagatccaa ctgcggcttg tttggctccg
     4921 tccgagtaga cgtcgatctt tttcgccgcg gtttaggctg tgcggtcgcg ccgttgggat
     4981 tttccagatg agcaagaatg tctttggccc gatcgagaat ttcttttggc aacccggcta
     5041 ggcgcgcgac ctggatgccg tagcttttgt ccgcgccgcc ggggacgatc ttgcgcagga
     5101 aaatgatctg gtcgttccat tcacgcacgg cgacgttgaa attcgctgcg ccagtgcgct
     5161 cggccgcgag cttagtcagc tcatgataat gtgtggcgaa gagcgtgcgc gctttgatct
     5221 tgtcgtggag aaactcggcc acgctccacg cgatcgacag cccgtcgaaa gtcgaagttc
     5281 cgcgcccgat ctcgtccaag atgacaaggc tgcgcgccgt cgcgttgttg acgatattcg
     5341 cggtctcgtt catttcgatc atgaacgtcg attgaccgcg ggcgagatcg tcgttcgccc
     5401 cgacgcgcgt gaaaatccga tcgaccaaac cgacctccgc gctcgccgcg ggcacgaagc
     5461 tgccgatctg cgccattaac gtgatgagag cgacctggcg gatgtaagtc gatttgccag
     5521 ccatgttcgg gccggtgacg atcagcatgc gaatcttctc gtcgagctcg gtgtcgttag
     5581 gcacaaattt ctcctcgacg aggttctggt cgagcacggg atgccgaccg tctttgatgc
     5641 aaagccggag cgaatcgttt agcactggcc gacaataatt aaacagacgc gctgtttccg
     5701 cgaatgagca gataacatcg acaatggcaa ttgcgtcggc ggtttgctgg atggcgccga
     5761 gttcgcgcag cgcttcctcg cgcagtttct ggaacaattc gtattccaaa ttccgtgctc
     5821 gctcatccgc gccgaggatc ttcgcctcca tctccttcaa ctccggcgtg acgaatcgct
     5881 cgccgccgac cgtcgtctgc ttgcgcgtgt aatgcggtgg cacgcttccg aggttcgctt
     5941 tcgtcacctc gatgaaataa ccgaacaccg agttgtagcg gaccttgagc gatttgatcc
     6001 cgctcgccgc gatctcgcgc tcctgtaact ggctgatcca gtctttgcct tcacgcgacg
     6061 cgcggcgcaa ctcgtcaaga tcgacatcgt atctgtcgcg aaaaatcccg ccttccttca
     6121 attgaaacgg cggctcatcc accagggcgc gcgccagctt ctcggctaac tccggcatct
     6181 cctcaatctc attctgcaaa tgctgcgcga gcgaggtgac ttcatccgtt tccctagcgc
     6241 gattggttcc gaatgcggcg cggtcgagca aggcttgcac gtgtcgtttc aactgcggga
     6301 tttgctgcag ggatgttttc agcgcgagga gatcgcgcgc gttccccgag gcctggctca
     6361 atcgaccaac cgcccgctcg atgtcgcgga tcgctttcaa ttgcgcgcga agcgccgcga
     6421 gcacgtccgg ctcgcgcaaa agatcggcaa tcatccgttg ccgccattcg agctccttca
     6481 ggttgcgcag cggctggaga atccaggagc gcagtttgcg tccgcccatt ggtgtgacgg
     6541 tgcggtccag caccgctagc aaactgacat cgcgcgcgct gcgcgattcg accagctcga
     6601 gattcgtctg cgtcgccgcg tcgatgatca cgtgctcgct cgcagcgtcg catttcagcg
     6661 agctaagatg atcaatcttt cgccgcagtt gatgtttgag gtaatgcacg agcgcacctg
     6721 ccgcggcgac ggcggcagac atctgcgtgc agccgaagcc atccagcgat ttcactttga
     6781 aatgctcgca aagcgtgaag ctcgcgtgct ccggcaggaa cgcatagctg tcgtaggaaa
     6841 tggcgcgacc gaacccggcg agctgttcct tctgttcctc cgggatcagc agttccgtcg
     6901 gtgcgacgcg cgccagctcg tccaagagtg attgccgatc tggcagttgc gcgagacgaa
     6961 attcaccggt cgttaggtca gcgtaggcga acccgtacac gcgattgtcg atgtaaacgg
     7021 cgccgagata atttgcgcgc ttcgattcga gcagatcaag atcgctcacc gtgccggcgc
     7081 tgatgatttg cgtaatgtcg cgatcaacga tccggccgga ttgtggctcg ctcgtttgat
     7141 cgcaaatcgc cacgcgcttg cccgccttaa tcagtttcgc gatataactc tgcgccgcgt
     7201 gatacggcac cccgcacatc ggcacactgt tccgcttcgt cagggcgacg ttcagcagcg
     7261 ccgacgcttc cttcgcatcc tcgaaaaaca gctcgtaaaa atcgccgagc cgaaaaagca
     7321 gaagcgtgtc cgccggaata ctcccccgca gccgccggta ctgctgcatc atcggggtga
     7381 gcgcgtcctg cattgcgcaa ttatgatgct aaatcctgca gcgaacgaaa agaatgtttg
     7441 agtgcagcaa tcggggaaaa ccgaattgta gggcaaccgc tccggttgcc ggtctttcgt
     7501 atcagcaggc gaagcgcctg cctgacaatt aaatcagatg agcattgggc gttggatgtt
     7561 tggcgttgag cgttggacgt tttcttcttt ttctcgtcac ccacgcgccg tcttaatccc
     7621 gaggcggatg atccgctcga tcaattgcgg ataagctagg ccggcgttgc ccgccgaaag
     7681 cgcgaagtct tcatccgccg cgagatgcgg gtttgggtta gcttcgatga agtagatttt
     7741 gttgtccggg gcaagccgca gatcgatgcg ggcgtagccg tcgatggtga gcaggcgata
     7801 gatgcgtttg caggtgtccg cgatctcgcg cgcgagcgcc ggctcaaggt cgtccgcgaa
     7861 gtgattctct aagccccaac gtttgcgata ttcctcgttc cacttggctt tgtaggtggc
     7921 gatcttcggc tcgttcggcg gcacttcgcg aaagaccagc tcgcggattg gaaacacctg
     7981 caaccgcgtg ttgccgatga tgctgacgta aagctcccgg ccttcaatat attcctcggc
     8041 aatcgcatcg ctgttgtgct tctcgtgcac aaacgcgacc cgctcgcgga attgctcctc
     8101 gttttggacg aacgatgcct gcgaaattcc gtaggaggcc tcgtccttca cgggcttcac
     8161 caaaatcggg aacttgagtt gcttaggccg cacgttgcgc tgcccgcgcg ggatgacgag
     8221 aaagttcggc acgccaatcc cgtggtagcc gacaattttc ttcgagatcc ctttgtcttt
     8281 gcacaacgtg agtccggtcg cgccgcaacc agtaaacggc acgtcttgca attctaaaag
     8341 cgaaacgatg ttttggtcga agcccgggtt atttttgaat tgttcgacca gattgaaaag
     8401 cagatcggct gggaacgtct ccagtttctg ccggacaagg tcgacatcgt cgaagatggc
     8461 gagctgctcg gccgtgtaac cacgctcgcc aagcgcagcg atgacgttcg cctctgtctt
     8521 ccaatcctcc gtcttcattt cctggctgag gtcttcgtcg atcttggtcg gccgaacgcc
     8581 gtcgcagagc accaggacct tgagcttcct tttcatgctc cctatttcgt gcgtttgaat
     8641 ttcccggtga agaggtagtt catcaccaag gtcgtgatgt aagaggcgac gcgaaaatcc
     8701 tgctgcggat catcattgtg tgcgtgcagc cccagctcat cgctgcgatc gatcagtcgg
     8761 ctaagcagtt tgttcacccg gtactttttc tcattcgtcc actgacacac cgaattcatc
     8821 aagcgtcggc gccgctgacg caggtacgag ctggctttga tgccctgcgg cgcgggaaat
     8881 aactgccgaa gatcgacatc gtaaaaaccg ggatagatat cttcgtacag ctttcgtttc
     8941 cgcgcgtaat aggttttgag cttgacgttc aggcaattgt aatcggctac gcgatacttc
     9001 ggcgcgtgca cgggcggttt gccggccaag gaatgcatga gcccatcgac atactccagt
     9061 tttctccgcg ccggccagcc agtataacgc tcgcgccaat cgagtcccgg cgtcagccac
     9121 acggcaaacg tctcggcgaa atcctcgtcc ggatgcgcct gcgcgtacca atcctccaaa
     9181 tgcacgacgt aactccggct gaacggccgt ggccggtaag tggtcggcgt gctttcgccg
     9241 gaggtgtggc cgaaacagtt ctgccatttt ttcttccgct gtagttgata ggcgtagctg
     9301 taagcgtgcc ccgcttcgtg gcgcattagt cgcatgaacc actcgggcgt ttcaccctcc
     9361 acctcgagca tcatcgtgcg ctccaacgcg cgtagccgct cgtgcacgag gaaaaaggga
     9421 acgaagatcg ccggaatacc gatcggcacg aaccattcat ctccgatgtg gcttggcgga
     9481 tgaaaggcca gtcccttggc tgagaggtcg tcgtaaagct gctttatcag cggctcaagt
     9541 gcggtgccct ccagccgcaa accgagcgtc gaaattcgtc gctcgagaag ttcttcgtcg
     9601 ctcagtgccg cccaatcggc aacgtccatc tcgcgcacgg tactagtcca acccgacgcc
     9661 gcaaatgacg tggaagcctc tgcaccgata gaaggaccga ttaaaatatt cgtaatcact
     9721 tctctcgttg ggagagggac ttatgactgg agtcgcgtcg gattgctgga caggtacgta
     9781 aagcgctgta aatacgtaga gatgaaaatg atcaatgcgc caattggcgc cgcccgccga
     9841 agcgggcagg ccggataaag gcgcgtttag atctcgccaa gttggagccc gcaattcgcg
     9901 gcaccccagt ggtcgaatct caccacgcgc gggtttttgc gccggtcacc tcgaaccgta
     9961 atcgggaatc acatttaaca atttagaatt atgaaaccaa cacctcaaat tagtgacact
    10021 cagaatgctt cgcggagttt tccgttaagc gattataact ttcaaccgac gatagaagcg
    10081 aagtcggtcg catcctctgc tccgcgagca acgaaagcgc ccgcgtttca cgaactaagc
    10141 cgcgaaattt ttgccgtgaa gacgacccgc gattacatcg cggagctttt ggtgttcgct
    10201 gcgatcatcg gccttgttgc ctggccagtc atcgcggcat tacacgcaat cacgcgtctg
    10261 gtgagaaatt actgattctg catctgggga gcgcacgcgc cccgcatgcc gagttcgccg
    10321 cctcgcccaa agaatcctct acacaaaagt tcgcgatcgc ccacacgcga ggcacgtgtg
    10381 ctccaagaca tttgctgccg agatgggcgc cgttacagct tcgcccgtag ctcctatttg
    10441 gccggaattc cggcttcaac gagacgttgc atgagttttt cgagcatcgc gcgatcgcga
    10501 taccattcct gagcacgcac gtactcgatg gtgaggtcgg gctgcgcggc gagtgcggcg
    10561 cgcgcgagct ttcacgcctg ctcgattccg ccgagggccc cgcgcgccgc gactcgcggg
    10621 acgatatcac ggacgaaatt actgtcacgc cttcgcgcgt atcgcagaaa cctgactggc
    10681 gacagacaaa aatttatcgg gccggcgtct gttacttgaa tctaactgtg ctggttagcg
    10741 cttctcacta ctgctgctca gatgactcgt tttgttttgc tacccgaccg gtcctaactc
    10801 tgcatgagcc cgctccgaca aaatagcgac gacccgaaag tactgaggcc gagcggtttt
    10861 gagccgagcc tttcggcgat ggacgttccg cgcaccggtg tcgcggcaca aaaacgaaaa
    10921 cgtcgcgtca tcatgattgc cgcaagtgcg ctcgctctgg tcacggccac ctttgcatta
    10981 tcgcggttga aaaccgcggt cccgagcgtc gagcgctcgg cggtttggat cgacacagtc
    11041 aaacgcgggt cgatgctgcg acaggtgcgc ggtctcggcg cgctcgtgcc ggaagagatg
    11101 cgctggattc ccgcgaatac cgaaggccgt gtcgaaaaaa tactcgtgtg gcctggaaca
    11161 actgttgaac ccgacacggt c
//
LOCUS       DASTDG010000067         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_965568, whole genome shotgun sequence.
ACCESSION   DASTDG010000067 DASTDG010000000
VERSION     DASTDG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965568"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFO30_02075"
     CDS             <1..666
                     /locus_tag="VFO30_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418102.1"
                     /translation="LTALWLRRVRPRVVLVSFDNHPDWDIRPPKWCCGGWVNRALELP
                     HVESASVWGCGNFECWWPANLFRNRRAERSGKLEVHPWADDRPVKDRQRRGAILRENW
                     REHFENFVLGIAGATVYVTIDLDCLSADEAVTNWENGRFTIVDLTWALAQLRRSTRII
                     AGDICGAFSKPAYARPKQRFASEWDHPKLVEPTATQIRKTNFAALERLWPALTTASRP
                     GGL"
     gene            complement(663..929)
                     /locus_tag="VFO30_02080"
     CDS             complement(663..929)
                     /locus_tag="VFO30_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9418103.1"
                     /translation="MELLNATGFRNRRSMSVLGVGIVSMTISFFLNLGLLQRFDLSYL
                     YPFQGLSIVIITLLAAAVLKEKLTWRLTCGVLLITAGVMLVSMS"
     gene            complement(1004..1408)
                     /locus_tag="VFO30_02085"
     CDS             complement(1004..1408)
                     /locus_tag="VFO30_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9418104.1"
                     /translation="MHERPRGVFNPWFQLGLNVLGVLAYELLLKAGANATADASKSWS
                     WTGITGLASPLTWLAIALIMLDLVIWLYVLKYIPLSIAFPLSRVVDALVPISCWIILE
                     ETISPRRWCGIALVVLGLAIVAKPAARLEERL"
     gene            complement(1442..>2619)
                     /locus_tag="VFO30_02090"
     CDS             complement(1442..>2619)
                     /locus_tag="VFO30_02090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418105.1"
                     /translation="GARLTNGVETTPETPNGVAEVVSRSDLGNLPEWQQAFRDRCKHH
                     RYYEIVADTLTGFEHHFLLLKERSGSIRAIQPFFFVTQNLVEGIPGPLRSFVDLVRKF
                     FPRFLTMRTLMVGCAAGEGHLGTLATADDTWVAETLRDVLPEIAQARKASLIVLKDFP
                     SAYREALRKLRTSGFARVPSMPLTRLSLNHNSFDDFFATLSKATRKDLRRKFRKADRA
                     PKIDMEVVSDITPHIDEVYPLYLAVHNRSPMKFETLTKDYFRRISRDMPDRARFFIWR
                     QQGKIIAFSLTLLCGDTIYDECLGMDYAVAFKLSLYFYTLRDVLAWAIQQRIKYYYST
                     PLNYEPKLHLHCELVPLDLYARHTSPLINPIFGQIVKFLGPTRHDQVLRRFPNADQL"
BASE COUNT          598 a          721 c          783 g          517 t
ORIGIN      
        1 ctgacggcgc tttggctgcg gcgtgttaga ccgcgagttg tgctcgtgtc cttcgataat
       61 catcccgact gggacattcg cccgccgaaa tggtgctgcg gcggttgggt aaaccgcgcg
      121 ctcgaactgc cgcacgtcga gagtgccagc gtgtggggat gcgggaactt cgagtgctgg
      181 tggccggcta acctgtttcg caaccgccgc gccgaacgtt cgggcaagct ggaggtgcac
      241 ccttgggcgg acgaccggcc ggtgaaagat cgacagcgtc gcggcgcgat tctgcgtgag
      301 aactggcgcg agcatttcga gaatttcgtg ctcggcatcg cgggcgcgac tgtttacgtg
      361 acgatcgatt tggattgcct gagcgcggac gaggcggtca caaattggga gaatggccgc
      421 ttcacaattg tcgatcttac gtgggcgctg gcgcagctgc gccgatcgac ccgcatcatt
      481 gccggggaca tctgcggcgc gttttccaag ccggcgtatg cgcgtcccaa gcagcggttc
      541 gcgtccgaat gggaccatcc gaagctagtc gagccaaccg ccacgcagat acgcaaaacc
      601 aattttgccg cgcttgagcg gttatggccg gctttgacaa ccgcaagtcg accaggaggc
      661 ctttagctca tagaaacgag cattactccc gcggtaatca agagcactcc gcaggtcagt
      721 cgccaggtga gcttttcctt cagcaccgcc gccgccaaca acgtgatgat gacgatgctg
      781 agcccctgga acgggtaaag atagctgagg tcgaagcgtt gcagcaaacc gagatttaga
      841 aagaaggaaa ttgtcatcga aacgatcccg acgccgagaa cggacatact cctgcgattg
      901 cgaaacccgg tcgcgttcag tagttccatc gctcgtttaa aaagcagttg accggcgacg
      961 aaagtgctta gccaaacaac gatgagaatc gatgaccaca gagtcacagc cgctcctcca
     1021 gccgcgcagc cggcttcgcc acgattgcca gtccgagaac aaccaaggca ataccacacc
     1081 atcggcgcgg cgagatcgtc tcttccagaa tgatccagca gctgatcggc actaaagcat
     1141 cgacgacgcg cgacaaagga aaggcaatgg agagggggat gtatttcaag acatagagcc
     1201 agatcaccag gtcgagcatg attaaagcga tcgcgagcca ggtcaggggc gaagccagtc
     1261 cagtgatgcc agtccatgac cagctctttg atgcgtcagc ggtagcgtta gcaccggctt
     1321 tgagtagcaa ttcatacgca agcacgccca agacattgag cccgagctga aaccacgggt
     1381 tgaacacgcc acgcggtcgc tcatgcatga tcgcgatgtt gcacgatatc gtcccgggga
     1441 atcaaagctg atcagcgttt ggaaagcgcc gcagcacttg atcgtgtcgg gtcggcccga
     1501 gaaacttcac aatctggccg aagatcgggt tgatgagtgg tgaagtgtgt cgcgcgtaga
     1561 gatcgagcgg caccagttcg cagtgcaggt gcaacttcgg ctcgtagttt aaaggagtgc
     1621 tgtagtaata tttgattctc tgctggatcg cccacgcgag cacatcgcgc agggtataga
     1681 agtagagcga aagcttgaac gcgacggcgt aatccatgcc gaggcactca tcgtaaattg
     1741 tgtcgccgca gagcagcgtc aggctaaacg cgatgatttt gccctgctgc cgccaaatga
     1801 aaaaccgcgc ccggtcgggc atgtcccggc tgatgcggcg aaaataatct ttcgttagcg
     1861 tctcgaattt catgggcgag cggttatgca ctgccagata gagggggtaa acctcgtcaa
     1921 tatgcggcgt gatatcgctg accacctcca tgtcgatctt cggagcacga tctgccttgc
     1981 gaaacttgcg ccgtagatct ttgcgggtcg ctttgctcag ggtggcgaag aaatcatcga
     2041 agctgttgtg gttcaatgaa agtcgcgtca gcggcatact cggtacgcgc gcgaagccgc
     2101 tggttctcag tttgcgcagt gcttcgcgat aagcagatgg gaaatccttc agcacgatga
     2161 gcgaagcttt cctggcttgc gcgatctccg gcagtacgtc gcgcaaagtc tcggcgaccc
     2221 aagtgtcatc agccgtagcc agggtgccga gatggccttc gcccgcagcg cagccgacca
     2281 tcaacgtgcg catggtgagg aagcgcggga aaaattttcg cacaagatcg acaaacgaac
     2341 gcagcggacc cggaatgcct tcgaccaaat tttgggttac aaagaagaac ggttggatcg
     2401 cccgaatcga tccagaacgc tcttttaaga ggagaaagtg atgctcgaac ccggttagcg
     2461 tgtcggcgac aatctcatag taacgatgat gcttacatcg gtcgcggaac gcctgttgcc
     2521 attccggcag attcccaagg tcgctgcgcg acacgacttc agcgacgcca ttcggcgttt
     2581 ccggggtcgt ctccacgccg ttcgttaggc gcgcccctc
//
LOCUS       DASTDG010000068         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_647112, whole genome shotgun sequence.
ACCESSION   DASTDG010000068 DASTDG010000000
VERSION     DASTDG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647112"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VFO30_02095"
     CDS             <1..1973
                     /locus_tag="VFO30_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9418106.1"
                     /translation="ARSTSIHIDYAAELNEQQLAAVTASPGPLLIIAGAGSGKTRTVT
                     YRVAYLLENGIDPRHILLLTFTNKAARQMLDRVANLLPIDASGIWGGTFHSIGNRMLR
                     RHGSALGYSSGFTIMDREDQKDLINTVVASAGIDPKEIRFPKGDVLAEIFSFVVNTET
                     PLEQLLAEKFPYFLPLLDKIQDVHARYEKKKKATNSLDFDDLLEKTLSMLQQHDRVAA
                     FYRRQFQFILVDEYQDTNKIQADLIDLLAREHRNVMVVGDDAQSIYSWRGANFQNILQ
                     FPKRYPDAQVFKIEMNYRSVPEILEVANAAIATNVDQFHKNLQATRESLGVKPALVAL
                     NDGSEQAQFVAQRILELRDEDVDLNQIAVLYRAHYHALELQLELSRRGIPYQITSGIR
                     FFEQAHIKDVVSFIRFVANPRDEVAFKRMVKLLPGIGNRSAENLWSAWEKSLAAAGEI
                     TSWSERLLAMTVSAKSKKHWTQLAHTLDEIAPGGQPNPPSEMITSVVEAIYDDYAKVN
                     FTNYELRREDLDQLAVFARQFADVQEFLAQLALISNVDAEAAPAQPADKEAVNLSTVH
                     QAKGLEFHTVFVIWLTDGMFPSGRSLDTRESLEEERRLFYVAITRAKDELYLTYPQMR
                     LSGGYGDIFQRPSRFLKEIPNNLVEDWQVQRS"
     gene            2016..2411
                     /locus_tag="VFO30_02100"
     CDS             2016..2411
                     /locus_tag="VFO30_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9418107.1"
                     /translation="MLEVEDSRSGKLLHGQTTQKIIGAAFEVHDQLGYGFLEHVYQRA
                     LQVELLRLGATAELEKRIQVQYKGVVVGDYDVDLVVDGCVAVEIKVAPQYDKRDEAQL
                     LNELKATGLKVGMLVNFGRAKVEHKRLVF"
BASE COUNT          578 a          702 c          760 g          512 t
ORIGIN      
        1 cggcaagatc gacatcgatc catatcgatt acgcggcgga gttgaatgag cagcagctcg
       61 cggccgtgac ggcatcaccg gggccgctgc tcatcattgc cggcgcgggc tcaggtaaga
      121 cgcgaacggt gacatatcgg gtggcgtatt tgctcgagaa cgggatcgat ccgcgccaca
      181 ttttgctgct cacgttcacg aacaaggccg cgcggcagat gcttgatcgc gtggcgaatc
      241 tgttgccgat cgatgcgagc gggatttggg gcggcacctt tcattcgatc ggcaatcgca
      301 tgctgcgtcg gcatggcagc gcgctcggtt attccagcgg gttcaccatc atggatcgcg
      361 aagaccagaa agacttgatc aataccgtcg tcgcgagcgc ggggatcgac ccaaaggaga
      421 tccggttccc gaaaggcgac gtgctggcgg agattttcag tttcgtcgtc aacacggaga
      481 cgccgctcga gcaattgctc gcggaaaagt tcccctactt cctgccgtta ctcgacaaga
      541 tccaggacgt gcacgcgcgt tacgagaaaa agaagaaggc gacgaactcg ctcgatttcg
      601 atgacctgct ggaaaagaca ttgtcgatgc tgcaacagca cgaccgggtc gccgcgtttt
      661 atcggcggca atttcaattc atcctggtcg acgagtatca ggacacgaac aagatccagg
      721 ccgacttgat cgatctgctg gcgcgcgagc atcgcaatgt catggtcgtc ggcgatgacg
      781 cgcagtcgat ctactcctgg cgcggcgcca atttccaaaa cattcttcag ttcccgaaac
      841 gctacccgga cgcgcaggtg ttcaagatcg agatgaacta ccgcagtgtg ccggagattc
      901 tggaagtggc gaacgccgcc atcgcgacca atgtagatca gtttcacaaa aaccttcagg
      961 caacgcgcga gtcgttaggc gtgaagccgg cgctggtcgc gctgaacgac ggcagcgaac
     1021 aagcgcaatt cgttgcgcaa cgcatcctcg agctgcgcga tgaagatgtc gatcttaacc
     1081 aaatcgcggt gctttaccgg gcgcattatc acgcgctgga gttacaactt gagctgtcgc
     1141 gccgcggcat cccctatcaa atcaccagcg gcatccggtt tttcgagcag gcgcacatca
     1201 aagacgtcgt ctcgtttatc cggttcgtgg ccaatccgcg cgatgaagtc gcgttcaagc
     1261 gcatggtcaa gctgctcccc ggcattggca accgcagtgc ggagaatctg tggagcgctt
     1321 gggagaaatc gctcgccgcg gcgggcgaaa tcacctcatg gagcgagcgg cttctcgcga
     1381 tgaccgtgag cgcgaagtca aaaaagcact ggacgcagct cgcacatacc ctcgacgaaa
     1441 tcgcgcccgg cggtcagccc aatccgccgt cggaaatgat cacgtcagtg gtcgaggcga
     1501 tttacgatga ttatgcgaag gtgaacttca ccaattacga gctgcgccgc gaggatctgg
     1561 accaattggc cgtctttgcc cgacaattcg ccgacgtcca agaatttctc gcgcagcttg
     1621 cgctgatcag caacgtcgat gcggaagcgg cgccggccca gcccgccgac aaagaggcgg
     1681 tgaatctctc gaccgtccat caggccaaag gcttggagtt tcacaccgtg tttgtgatct
     1741 ggctgaccga cggcatgttt ccaagcggcc gatccctcga cacgcgcgaa tcgctcgaag
     1801 aagaacggcg tttattctac gtcgcgatca cccgagccaa ggacgagctg tacctgactt
     1861 atccgcagat gcgcctgagc ggcggttacg gcgatatttt tcagaggcca tcgcgtttcc
     1921 tgaaagaaat cccgaataat ctcgtcgaag actggcaggt gcagaggagc tgagtctggg
     1981 ccacggacta acacggatga aacacggata agcagatgct ggaggtcgaa gattcaagat
     2041 ccggcaagct gcttcacggt caaacgacac agaaaatcat cggagcggct ttcgaggttc
     2101 acgatcaact cggctacgga tttcttgagc acgtttatca gcgcgcactc caggtagagt
     2161 tgctccggct cggtgccact gccgaactgg agaaacgaat tcaggtccaa tacaaaggtg
     2221 ttgtggtggg tgattacgat gtcgatcttg tcgtggacgg ctgcgtcgcg gtcgaaatca
     2281 aggtcgcacc gcaatacgac aaacgcgacg aagcgcagtt actgaacgag ctgaaggcca
     2341 ccggactgaa agttgggatg ctcgtgaact ttgggcgagc caaggtggag cacaagcggc
     2401 tcgtcttctg aatccgtgtt tcatccgtgt tcatctgtgg ctgaagggaa ttgatgcacc
     2461 tttcaccgga gaagaagatc gcgggaattc ttgcgccgct gtttgggttg cgcggggaga
     2521 acgatctcgg cattggcgat gtcgcggtgt tg
//
LOCUS       DASTDG010000069         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_774912, whole genome shotgun sequence.
ACCESSION   DASTDG010000069 DASTDG010000000
VERSION     DASTDG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774912"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFO30_02105"
     CDS             complement(<1..939)
                     /locus_tag="VFO30_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9418108.1"
                     /translation="MRFPLALTTKIAAYLIAQKLRGTKKFATVLQLEPLHTCNLTCTG
                     CGRIREYSTSLKDMMSLEDCLSAAEECNAPMVSICGGEPLIYPHIEVLVSGLLAQKRI
                     VYICTNAMFMRKKMREWMAAEYRKANTAKQKDIEDKIDSLMSNGLLSDKDAAEIRKGP
                     KDPAKPTIAPNKWMYWNVHLDGVERTHDLIVEREGVFKECILAIKMAKLLGYQVATNT
                     TVYKEADMREIEQMFDYLADLGVDGHTITPGYDYDAAKKDMIKRLSLRPENFFLTRDM
                     TVQKFQKIDEWMNRYTFFGTPIYFEFLAGKRDLTCSA"
     gene            complement(1053..>2402)
                     /locus_tag="VFO30_02110"
     CDS             complement(1053..>2402)
                     /locus_tag="VFO30_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418109.1"
                     /translation="DQSHSPRLASDMNQLGAVHAVRGGTLLAVVIVLAVYGLEAHARR
                     EAVPTPMMHSDQAAYLAYARQMRESDYTFTAARNRMPVFPFLLSLLYQPGMDEAQFLK
                     RAQSFNINLSILLLVLLFVIFRKFFPSYHALALLVITAFGVFVYRTAIVQVEPLYYFL
                     SFCAFLFLVRLLIVPGWWLSIFAGATLGLAYLTKASMLPTLPLWVGVFLVQIISRFRH
                     DNGRRVVARDFGLLLLVLASFLVVVFPYIQTNKRLYGHYFFNVNSTFYMWCDSWPEAV
                     AFTEIYNDPKRRRELAPNELPSAAKYWREHSLGQIVHRLGNGLASMLKRSAKPTGYYK
                     FVLLFAVAGAFALRRGESTIAFVKQRAFAASFCILFMVVYVLLYAWYGAIITDSRFIL
                     SLFLPFIFAGTVFLLRLCHGQSYAIGGRQFALAELFAGSLIALAAVDAAYNARHFFG"
BASE COUNT          576 a          657 c          683 g          486 t
ORIGIN      
        1 ggccgagcag gtcaggtccc gtttgccggc aaggaactcg aagtaaatcg gcgtaccgaa
       61 aaacgtgtag cgattcatcc actcgtcgat tttttggaac ttctgcacgg tcatgtcgcg
      121 ggtgagaaag aaattttccg ggcgcaagct cagccgctta atcatgtctt tcttggcggc
      181 gtcgtaatcg taaccgggtg tgatggtgtg gccgtcgacg ccgagatcgg ccaggtagtc
      241 gaacatctgc tcgatctccc gcatatcggc ctctttgtag acggtggtgt tcgttgcgac
      301 ctggtagcca agcagtttcg ccattttgat ggcgagaatg cattccttga acacaccctc
      361 gcgctcaacg atcaggtcat gcgtccgctc gaccccgtcg aggtgcacgt tccaatacat
      421 ccatttgttc ggagcgatcg tcggcttggc cggatctttc gggcctttgc gaatttcggc
      481 ggcgtctttg tcggagagca atccgttcga cattaagctg tcgatcttgt cttctatgtc
      541 tttctgcttt gcggtattcg ctttgcggta ttcggccgcc atccattcgc gcattttttt
      601 gcgcatgaac atggcattgg tgcagatata gacgatgcgc ttttgcgcga gtaacccgct
      661 caccagtacc tcgatgtgcg ggtaaatgag tggttcgccg ccgcagatcg agaccatcgg
      721 cgcgttgcat tcctccgccg cggagaggca atcctccagg ctcatcatgt ccttgagcga
      781 agttgagtat tcccggatcc gcccgcagcc ggtgcaggtg agattgcacg tgtgcagcgg
      841 ctcgagctgg agcaccgtgg cgaacttctt tgtcccacga agcttctgcg cgatcaggta
      901 agccgcgatc tttgtcgtta gggcgagggg aaatctcata aaagcgtcga cggtatcaac
      961 gctgccaatt gtgtcaaccg ctggctgatt tgatcaagta agctaaaacc atggccgaag
     1021 ctagcaaatg atgcggcctg cggctcggcg cgtcagccga agaaatgccg cgcgttgtac
     1081 gcagcgtcaa cagcggccaa agcaatgaga ctgcccgcaa aaagctccgc cagcgcaaat
     1141 tggcgtccgc cgattgcata actctggccg tgacagaggc gcagcaaaaa cactgttccg
     1201 gcgaagataa acggcaggaa gagcgagaga atgaatctgc tgtccgtgat gatcgcgccg
     1261 taccaggcat acaaaagcac gtaaaccacc atgaaaagga tgcagaaact cgcggcgaac
     1321 gcccgctgct taacaaatgc aatcgtcgat tcacctcgac gcagcgcaaa cgcgccggcc
     1381 acggcaaaga gaagcacgaa cttgtagtaa ccagtcggct ttgcgctcct cttcagcatg
     1441 ctggcgagac cgttaccgag ccggtgtacg atctggccga gcgaatgttc gcgccaatat
     1501 ttcgcagccg acgggagttc gttcggcgca agctcccggc gtcgcttggg atcattgtaa
     1561 atttctgtga acgcgacagc ttccggccac gaatcgcacc acatgtaaaa ggtcgagttc
     1621 acgttgaaga agtaatggcc gtaaagacgc ttgttcgtct gaatgtatgg aaaaaccacc
     1681 accagaaaac tcgcgaggac gagcaagagc aggccaaagt cgcgcgcgac gacgcgcctg
     1741 ccattgtcgt gtcggaatcg cgaaatgatt tgaacaagaa acacgccgac ccaaagcggc
     1801 agcgtcggaa gcattgaagc cttggtgagg taggccagac caagcgtcgc gcccgcgaag
     1861 atcgacaacc accaacccgg cacgattaat aacctgacga gaaacaggaa cgcgcaaaag
     1921 ctcagaaaat aatacagcgg ctccacctgg acgatggcgg tgcgatagac aaacacgcca
     1981 aatgcggtaa tcacgagtag cgcgagcgcg tgataactcg gaaaaaattt ccggaaaatc
     2041 acaaagagca ggacgagcag taagatcgac aagttgatgt tgaacgactg cgcgcgtttc
     2101 agaaactgcg cctcgtccat tcctggttgg taaagcagcg acagtaagaa aggaaatacc
     2161 ggcatccgat ttcgcgcggc ggtaaaggtg taatcgctct cgcgcatttg ccgggcgtac
     2221 gcgagatagg cggcctgatc gctgtgcatc ataggagtcg gaacggcttc gcgacgcgcg
     2281 tgcgcttcca acccatagac cgcgagcaca atcacgaccg cgagcaatgt gccgccgcgc
     2341 acagcgtgaa cggctccgag ctgattcatg tcgcttgcca gccgcggcga atgagactga
     2401 tc
//
LOCUS       DASTDG010000070        32111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1311726, whole genome shotgun sequence.
ACCESSION   DASTDG010000070 DASTDG010000000
VERSION     DASTDG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 32111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32111
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311726"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VFO30_02115"
     CDS             complement(<1..1567)
                     /locus_tag="VFO30_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418110.1"
                     /translation="MDRIAEENGAADGEHEPSHRTQDRSPELRHGEDQTRSQAAAPPS
                     KTPDAKLIQVTGSGLSGPFIQRPVMTVLLTLAVIVAGLATYNKLPVNDLPAVDYPIIQ
                     VTCSYPGASPETMANNIATPLEKQFLQIPGLDLITSASSQSNTSLTLQFNLSKSITDA
                     ATDVQAAIQRATGKLPIDLPSPPTFTKTNPNDQAVYLLGLTSDTLTDGDLYKYASTAV
                     AQRISILPGVSQVNIYGVQGAIRIKADPAALAARGLTMDDLASAIKAGTVYSGAGQFD
                     GAHRTFVLQPNGQIDDADGYRNLIVAKNRDGSPVHLRDIADVRQSVQDERMSRTFWAR
                     GFHPPGSVVVLAVSRQAGANAVEVANSVKALFPELLASLPGSITLVPVFDRSQTIVNS
                     VHDVRFTLVIAFMLVVMVIYVFLGRATDTLIPIVALPLSLLLTFAVMYVLNFSINNLT
                     LMALTLAIGFLVDDAIVFLENVVRRSEHGESIVRAAYNTAGEISFTILSMTLSLAAVF
                     IPLVFLPGLLGRIF"
     gene            complement(1572..2717)
                     /locus_tag="VFO30_02120"
     CDS             complement(1572..2717)
                     /locus_tag="VFO30_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9418111.1"
                     /translation="MKRQLARSQTIPGWAYLLFLVLAAFALYRVVQHRAQEGKAAQPP
                     KRPVLVAQVTTKDVPLYLDEIGTCAAYETVQVQAQVSGQIMARHFQDGAEVKQGDLLF
                     TIDPRPYQAALDQARAQVALDQATLKRQQQLRARNVVSPQDLDTAQANASKSEAIAAA
                     AQVNLDFCYIKSPINGRIGLRNVDVGNLVGPSSPPLVTIQGLDPIYTDFTVAETDLAL
                     VRKYLGSPNLKVQTWSPDANIAPRLGDLYFIDNAVQPGSGTVKARAVTPNPDRALWPS
                     EFVRVRFILDTIKDATLVPSQAVQISQSGPYVFVVKPDDTVELRPVKPGQRQDADLTV
                     IESGVKPNEAVVVTGQLALGPGTKVDPKPYNGPVGPTSDSSATKSAL"
     gene            complement(2714..3265)
                     /locus_tag="VFO30_02125"
     CDS             complement(2714..3265)
                     /locus_tag="VFO30_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9418112.1"
                     /translation="MSQIDPEIFGPLLHGTARAWRLKLDERLKPMGLSQAKWRTLLHL
                     SIAPEALTQTEIAERLGIEEPTLVTLLHRLEREGWVKRANAAHDRRCKLVLLGRRAQS
                     VISHINAAADELRHELLADVAASELQTCIDVLTRIRARAEKRDKARPNGLGAGASAGN
                     GEKSNGQRITKQRERWPSAGGAK"
     gene            complement(3324..4754)
                     /locus_tag="VFO30_02130"
     CDS             complement(3324..4754)
                     /locus_tag="VFO30_02130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9418113.1"
                     /translation="MSRLPSWLLVVVAWAAIYLPGLGSFEIKGEEGRRILPAVAMLES
                     GNYLVPTVGGAAYYRKPPLVNWLVAVSFKLFGHRNEWTARAPSVVCVLAVALAFLTVA
                     RGSVGSRGATIAALVWLTNFGMIEKGRLIEIEALYISLCALAIICWLSWWEQKRSPWL
                     TWTVPWIFLGLGWLAKGPTLLFFFYAVVVAVLWRDKQWRFLIHPAHLVGWLIMLAVFG
                     LWAVPFLQTESSPLIMRKWLGQYAGRVTGEFLRLANWFEMLSRSLIYFLPWLVFVPWL
                     RLRRFAEARQRDLARALAWSIVVPFLLINLTPGMAPRYCLPVLVPFCLLTGLSFAEHA
                     FAPLPWLKQPPELLWSRVGQPLVAFVAAAVLVGYPLAAAMIFKHRPKVKNMAEQINSI
                     VPRNELLYAVDPNYQPFLFYVDAAVRYVSSVAELPPDTHYFLVLPEDEESASNSQQWR
                     PRRARPVLRFTDYRYETVILFAVESS"
     gene            5008..6282
                     /gene="nusA"
                     /locus_tag="VFO30_02135"
     CDS             5008..6282
                     /gene="nusA"
                     /locus_tag="VFO30_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9418114.1"
                     /translation="MNAELIAMLDYLERERGIKRDILLEAVSNALLSASKKSVSVSRD
                     LRIDINPTTGVIRALANPLVVETVSNPQDEISLAKARLINPNVKVGDSVEMEVTPRNF
                     GRIAAQTAKQAMMQRIRQVEKEMIYEEFKDRAGEIVSGTVRRFDRSDVILDLGKFEAI
                     MPQRERVVVEDYNIGDRLRAYVVAVDNGIRGPEIIVSRSHPNFVRRLFELEVSEIADG
                     TVEIRGIAREAGYRTKIAVWSANEKVDPVGACVGMRGSRVKNIVRELNNEKVDIIRWS
                     SDPKEYILEALKPAKVKNLLFDTEKKSVQISVDEDQLSLAIGKKGQNARLTSRLTGWE
                     INIDKDTSATTAVEQKVAQAAQTLAGALPVTQEQAVTLVKSGFTNLEGLRHADVEDLV
                     DILGVDQAKAREIHEAVNREGAEEKVGSAQSA"
     gene            6311..8572
                     /gene="infB"
                     /locus_tag="VFO30_02140"
     CDS             6311..8572
                     /gene="infB"
                     /locus_tag="VFO30_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9418115.1"
                     /translation="MPTKSIKKPATRKTAASKTVKAAVSKPAAKSAAPKKVERKPARP
                     DRAARAENAGAAPLPEAPAKPAEPLTGRHEVKSVSLIDAERPENKSDKDGQLKTKSTI
                     LPPISRIRASFETPTVPPVPKIDKIAPPETARPAELTTEPTPVVPDGIAAPPEPAPEE
                     EAQKVIHIKPPIIVKQLATELGLKPHQLIAELMTFNIFANINQTIEPDIAAKIAENHG
                     FVLEKERREKGGGVHKVEQVVVAPPPPLITPEEELKSRGPIITFMGHVDHGKTSLLDA
                     IRKTRVAAGEVGGITQHIGAYQVDYKGTKITFIDTPGHAAFTAMRARGANVTDIVVLV
                     VAADDGIMPQTVEAINHAKAAPHVKIMVAINKIDLPGANIDKVKKQLQEHGLTPEDWG
                     GETIVCPVSATKGTGIDHLLEMMTLQAEVMELKASPTAKPRGTVIEAQVEPGRGPTAS
                     VIVQMGTLKIGDPFICGDYAGKIKSLMDDRGQPVRAAGPATPVKVVGFTGLPNAGDEF
                     LVMDSERAAKTLSEERLEAKRANKLAVPQRATLESLLEGPAGKKTLRIVLKCDVQGSV
                     EAIVGALQHIESKKVDLEIIHSAVGPISESDVLLASASNAVVVGFNVKVESMAVPAAK
                     REGVQVKLYSIIYELIDQIKEAMAGLLEPEHRETIIGHAEVKQVFQLSRGIVAGCLVT
                     DGRVSRSARARVLRKRQPVYDGGISTLRRFQDDVKEVRSGLECGVKLGDFSEYQVGDI
                     IECYQLEAVAQKL"
     gene            8569..8919
                     /gene="rbfA"
                     /locus_tag="VFO30_02145"
     CDS             8569..8919
                     /gene="rbfA"
                     /locus_tag="VFO30_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9418116.1"
                     /translation="MKHRQVRVNEVVKRELSGIIAREIRFEDALVSINEVDVAADLKS
                     AHVFVSVLGTATGASVIEQLEEHRPDLQTALARRVVLKYTPHLVFHLDESIQRGTRVI
                     QILQDMEAAAGENE"
     gene            8916..9902
                     /locus_tag="VFO30_02150"
     CDS             8916..9902
                     /locus_tag="VFO30_02150"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9418117.1"
                     /translation="MSAVNASFKEIARAICQHQRIAVLSHVRPDGDALGSQIALALSL
                     QQIGKDVRVWNEDGMLEKYSFVPRAELVTKPPAEPEDVDLAIALDTAIQNRLGTALTA
                     IRATKLWINIDHHLSNPRYGDLVHIDDSAPATGQILFDLITSEKLPIDRDIAENLYVA
                     ISTDTGSFQYPKTTSRTFEIAAELVRVGLDVGAISTQLYENFPRRRVELLRELLRTMR
                     FDGNGRVASFKLSLQTANELGVIPEDNEGLIDHLRAIRGVIVAVFFEELPDGKIRVSM
                     RSKDERIDVCAICQKFGGGGHTLAAGARIRGTLSEVEERVLQAACDAVDRQS"
     gene            9877..10602
                     /gene="truB"
                     /locus_tag="VFO30_02155"
     CDS             9877..10602
                     /gene="truB"
                     /locus_tag="VFO30_02155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9418118.1"
                     /translation="MTPSTANPDGVLLVDKAEGMTSHDVVALVRRKLEMKKIGHCGTL
                     DPIATGLLLLTLGRGTKVQDLLMSEDKEYVGTLTLGIITSTQDRQGEIVDQRPVPALT
                     DDQIRAAFEKFRGDFYQMPPMVSAKKHGGVPLYKLARQGKVIEREPRLVHVYRYTIDH
                     VALPDIDFSVLCSKGFYVRTYAHDIGETLSCGAHLKSLRRTKSGRFTVDQAITIHEIK
                     NSPVEQITNRILTLPEVSRMRGA"
     gene            10620..11546
                     /locus_tag="VFO30_02160"
     CDS             10620..11546
                     /locus_tag="VFO30_02160"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9418119.1"
                     /translation="MEILQSISQLEKLRGPVFLAIGVFDGVHRGHQAVISTSASHAQA
                     GGTAVVVTFDPHPAKVLRPHDAPHLLTATEHKIALIRELGVEHLLLIKFDRQFASTAP
                     KDFIAQLVRHSNPLREICVGHEWSFGKDRRGNVDLLRKLGAQYGFEVVSIPPVKVNGT
                     VVSSTAIRQAIEIGDFAKAARMLGRDYTILGTVTSGERLGKKLGFPTANLSAHSEQFP
                     PNGVYCAEAKLDGQLLRGVVNLGYRPTVSAAKDERVLEIHLLDFNRDVYGKDIEVRFT
                     RHLRPEKKFESVDALVRQIEMDVKQARELCTG"
     gene            complement(11562..11921)
                     /locus_tag="VFO30_02165"
     CDS             complement(11562..11921)
                     /locus_tag="VFO30_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418120.1"
                     /translation="MNGMIDDETLDRQLREAGPYIDDDGFTARIMAKLPAARPEPWQL
                     RAIILIGLTLLGSAIAYLLSGGGRFVSEGIIQLSSFPIWLLLVFVFGCGLLVGAFAVI
                     FALRKTPEVRDVARLWP"
     gene            complement(11926..12462)
                     /locus_tag="VFO30_02170"
     CDS             complement(11926..12462)
                     /locus_tag="VFO30_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9418121.1"
                     /translation="MPLTDAELVARVLVDDDHHAFAELVRNHQSTVRGLLRQLTGHDI
                     SLADDLAQETFLRAYKNLRSFRGEARFSTWLYRIAYNCFREYARKRRELVGVDEKQLE
                     RERDPQTVDPALRHDLMHALSLLPLHERTAVVLCCQNGLSHDEAARVLDIPLGTVKTN
                     VLRGREKLKRTLAAWGPN"
     gene            complement(12472..13119)
                     /locus_tag="VFO30_02175"
     CDS             complement(12472..13119)
                     /locus_tag="VFO30_02175"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HET9418122.1"
                     /translation="MKTLLSVSIGIAALATVVFAQPPATPQPGSPALSPSVAASPSVT
                     ASPASDLAEKIQRRIEEKFGGRHGVMIDTDELDRHDHEDVPGEVFPIVGISMLAVFGA
                     PVLMIAVLGYVAISKNRRLHRTIQMMVEKGQPVPAELLAPGKLPARQRSDMRRGVILV
                     MLGVGLMLWLAAVSDWEGGAWTLGLIPFLIGAGYLIVWKLEGGAKRRPDNPPPLP"
     gene            complement(13290..14603)
                     /gene="aceA"
                     /locus_tag="VFO30_02180"
     CDS             complement(13290..14603)
                     /gene="aceA"
                     /locus_tag="VFO30_02180"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET9418123.1"
                     /translation="MQRRADKDGRFNGIERPYSAAEVERLRGTVAIEHTLARRGAEKL
                     WNLLQTKDFIPALGALTGNQAVQQVRAGLKAIYLSGWQVAADANLAGQMYPDQSLYPA
                     NSVPAVIRRINNALLRADQIDHAEGPPQRVRPADKIDPPPPGYGAVGIDWLVPIVADA
                     EAGFGGPLNVFELIKAMIEAGAAGVHLEDQLASEKKCGHMGGKVLIPTSHAIRNLIAA
                     RLAADISNVPTIIIARTDANGAKLLTNDVDERDHQFLTGERTPEGFFCVRAGLEQAVA
                     RAINYAPYADLVWCETSAPNLDEARRFAEPVRERFPNKLLAYNCSPSFNWKKKLDRAA
                     IAKFQRELAAMGYKFQFVTLAGFHTLNYSMFNLARGYAARGMAAYAELQEAEFAAEEF
                     GYTATKHQHEVGVGYFDAVAQIICGGECSTAALRGSTEEEQFVKG"
     gene            complement(14653..16239)
                     /gene="aceB"
                     /locus_tag="VFO30_02185"
     CDS             complement(14653..16239)
                     /gene="aceB"
                     /locus_tag="VFO30_02185"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HET9418124.1"
                     /translation="MPRGIEIKGEITPEFAEVLTPEALAFVAKLHREFAARRKEALKA
                     RQDRQLRFDAGEMPDFLSATEHIRESDWTCALIPNDLRDRRVEITGPTDRKMVINALN
                     SGAKMYMADFEDANSPTWRNMVEGQINLRDAIRRTITFRSPEGKDYKLKNELAVLLVR
                     PRGWHLDEKHVVVDLAPVSGGLFDFGLFFFHNARELLERGTGPYFYLPKMENHVEARI
                     WNDVFNLAQDELGIPRGTIRATVLIETIPAAFEMDEILYELRDHSAGLNCGRWDYIFS
                     CIKKFRAKKDFILADRALVTMTTHFMHSYSLLCIKTCHRRNTFAMGGMAAQIPVKDDP
                     KANEEAFAKVRADKEREAGDGHDGTWVAHPGMVQLATDAFNAVMKTPNQIDRKRDHVN
                     VTADDLLDFAPREPITEAGLRQNVSVGVQYLEAWLRGHGAVPLFNLMEDAATSEISRA
                     QVWQWIRHPDGKLTDGRKVTKELFAHVLDEELGKVRNQLGQSIFEKRKYTLARELFAK
                     ITTDDEFAEFLTLPGYEYMD"
     gene            16382..16456
                     /locus_tag="VFO30_02190"
     tRNA            16382..16456
                     /locus_tag="VFO30_02190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16414..16416,aa:Gly,seq:gcc)
     gene            complement(16822..17826)
                     /gene="aroF"
                     /locus_tag="VFO30_02195"
     CDS             complement(16822..17826)
                     /gene="aroF"
                     /locus_tag="VFO30_02195"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9418125.1"
                     /translation="MLIVMNANAAPAEIDNVVRVAESLGCHAHVMPGASRTAVGLTGN
                     GEPLDTARFANLPGVTEAIRVTNPFKLTSLDARPERTVVDVKGAQIGAGSLTIIAGPC
                     AIENREQAFAVGEAVQRSGAQFFRGGAFKPRTSPYSFAGLGEEGLKILAEVRAKFGLR
                     VVTEALDESTVGLVEQYADMIQIGSRNMQNFTLLRRAGRSRLPVLLKRGMAATLEEWL
                     LAAEYVMSEGNNQIVLCERGVRTFSQHARNTLDLAAIPAMRRISHLPLIVDAAHGTGH
                     NYMVSPLARAGVAAGADGVMIEVHPRPESALSDGEQTLRIDEYHRLVEQLHGIREAIT
                     "
     gene            complement(17874..18812)
                     /locus_tag="VFO30_02200"
     CDS             complement(17874..18812)
                     /locus_tag="VFO30_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET9418126.1"
                     /translation="MKLRGIHHITAIATEPQRNLDFYTNVLGLRLVKRTVNFDDPSTY
                     HFYFGDRTGRPGTVITFFPWPGARRGTRGVGQVVATSFAIPQSALDYWKARFEEQRVL
                     CEQTSPRFGDNVLRLADPDGLLIELITSPELEDVDLGYKSDVPNEFAIHGFHAATLEV
                     QHATKTANLLETFGFEIVAEDNSRRRFSVDEPSTSAAVDLMERPDGSFGVNSAGTVHH
                     IAFRCADEAEQLEWRKRIVDLGLYITPVIDRQYFHSIYFREPAGILFEIATDGPGFTA
                     DEPVEHLGGSLKLPPQYEAHRAEIERILPPVNVLAR"
     gene            18868..19599
                     /locus_tag="VFO30_02205"
     CDS             18868..19599
                     /locus_tag="VFO30_02205"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9418127.1"
                     /translation="MHRSALESRFGDKLAVIAQPGRKRLEIQIAVNSMSRLRELVKIA
                     DGRIEKLPRDWLERFQRRDKPKLIGIKDRQLIIPAGAAFGTGEHATTAMSLHMLERLA
                     QTCSDGWSSIDLGTGSGILALAARLFGAKPVIGIDNDPRAVSTAKQNARLNKIDNVGF
                     RAADVRSCQLPRNIDIITANLFSELLVEILPKLRRAAWLILSGILREQERELIHALKR
                     QKINIVQVRRRGKWVTILARGLRRS"
     gene            19612..20700
                     /locus_tag="VFO30_02210"
     CDS             19612..20700
                     /locus_tag="VFO30_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9418128.1"
                     /translation="MKIGMQSDELAYAVVTPYSMRKSRTGGIVARLISRTGLDLVAAR
                     MFAPSKELTERYAKTIVTETEPAHRATQELIRDYVLKNFTGEKNGHRPRALCLVFRGP
                     DAIEKIHRAVGHIVHERTSGETIRDTFGDYITDDSGRVTYFEPGVLAAFDPSAVARDL
                     KLWAEFSDRDGGVLDQAVQFAPGTKIEKTLVLIKPDNFKFPNLRPGGVIEVFSRTGLY
                     IVAFKVHRMSVAQAEEFYGPVLPELQEKLGPAKGRSAWEDIVAFMAGARPSDTLPENR
                     EKPGTEKCIAVVYQGENAVAKIREVLGPTDPAKAPPGSIRKEFGQNIMVNAAHASDSV
                     ANAKREMTIVRVEENNFKPLIENFYRRQ"
     gene            20736..21899
                     /locus_tag="VFO30_02215"
     CDS             20736..21899
                     /locus_tag="VFO30_02215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9418129.1"
                     /translation="MEISERAGQLTPSLTLSIDSKAKAMKAEGIDVCGFGAGEPDFDT
                     PEHIKAAAIAALQAGFTKYTPSSGIPELRQAIAEKLAADNGLTYRATQVVVSNGAKHA
                     CYNAILATSQPGDEVIIPSPYWVSYPDMVRLAGAEPVIVPTFERNAWKMRAEDFENAM
                     TPRTKMLILNSPSNPAGSVYTREELQAIVDVAAEEDIYVLSDEIYEKLIYDNAQHVSI
                     GSLSKEAYDLTITVNGFSKSYAMTGWRLGYLAAPDAVAKAVESIQSHSTSNPSSFSQH
                     GALAALKGDQQPLADMREEFDMRRNYMYDRISKIPNVTAVKPQGAFYVLVNVSQLGLT
                     SQNFADRLLSKANVAVVPGAAFGDDRLVRLSYATSIDVIKKGLDRFQDFCRTL"
     gene            22079..22786
                     /locus_tag="VFO30_02220"
     CDS             22079..22786
                     /locus_tag="VFO30_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET9418130.1"
                     /translation="MPGHVSTDKAAVVARIKAEGAVAVIRTDSPERALAAAHAVVAGG
                     FRAVEITYSFAGATDVIAKLAEANENNLLVGAGTILSREQVHEAVGAGARFLVSPCML
                     PDVIDVAREHEVAVVPGAFTPTEIFTAYSLGADIVKIFPAVRFGPEYLRAVRGPLPHI
                     PIMPTSGVEACNVAEWFRAGAVAVGAVGSVLDPILIRNGEWDALTNRAREFIAAVRAA
                     RTGDQVANGTRSAKTAR"
     gene            22783..24360
                     /locus_tag="VFO30_02225"
     CDS             22783..24360
                     /locus_tag="VFO30_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET9418131.1"
                     /translation="MSKGHLALILHAHLPFIRHPEHEHFLEEEWFFEAATESYIPLLQ
                     MQQRLVNDAVPFKLTISLTPTLTAMLQDELLRERYVQHVDLLIDLAAREQKRNRKDAR
                     LRELAHFYSRFLSATRRFFVDECKCDLLAAFRKLRRAGVLEIIASAATHGLLPLLAQQ
                     SPAAARAQVLIGRDQYVGLWSEEPKGFWLPECAYAPELDRILQETNIRWFVLDAHGLL
                     FGNPQPCRAIFAPCYTRRGPAAFARDRDSSRQIWSAHEGYPGDPAYREFYRDAGFDLP
                     LEHLGPVAAGRRKFSGLKYHRITGRGPEKELYDRAAAEHRAQTHAAHFLEQRREQLRR
                     LNQQSADPIVVIPFDAELFGHWWFEGPLFLEWFIRKTASEQDDFCLTTPSEYLAAHPT
                     QQTIEPARSTWGENGDLSVWINPANAWIYPHLHGAARRMTEVARAHANEPSSLGDRVL
                     KQLTRELLLAQSSDWAFLMKTGTAREYATKRTIDHLGRFNRLHDQFAAKRSDEVFLRD
                     CEERDNLFPDVNWRYYA"
     gene            24353..25048
                     /locus_tag="VFO30_02230"
     CDS             24353..25048
                     /locus_tag="VFO30_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418132.1"
                     /translation="MPELQIANCRLQIVAVWLLVIGAAAQESSTPPISVTPTPPPSSA
                     RTVRISFVPPPLEGTISLGIFDRTGKLVRVLHQEAELDEFTVEADGLETKWDGKNDDG
                     KGLPAGKYRARGYTVGCKVEELGTSPNGLPSNTTEHVTVKLVANPLSKGANLVVELAV
                     GFDDENCFLQTTDGLPLLTVSETPNLWRAFVAKSGERSVDVFQDDGDTIDQLRVSNVN
                     QMMAFDCGEIELK"
     gene            25140..26456
                     /locus_tag="VFO30_02235"
     CDS             25140..26456
                     /locus_tag="VFO30_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HET9418133.1"
                     /translation="MTTEAQQTLAKPAGFSGTSLHTGEKVALKLQPAPVDHGIKFKRK
                     DLAEEPLIDAKIDNLKTVERATTIGEGAVRVHTVEHVLAALSGMGIDNAIIEMDSNEP
                     PIGDGSAQPYVDVIKRAGITSQDAPRKFFESREPVHIESKTGALLVLLPDEKFRVSCT
                     QAGPNNRFTQFLSMEITPALFEREIAPARTFVYYEDVRALMDKNLIKGGSLENAIVIR
                     DNAVLGKEPLRFEDEFVRHKILDIVGDLALLGRRVRGHLIAVKPGHAVNADLARVLAR
                     EQARRSEMEVPRAMPRGDGGLGTDEIMQVLPHRYPFLMVDRVVEFQGDNKITGVKSVS
                     INEPFFQGHFPGHPVMPGVLLVEAMAQVASILLFKLAKTSSRIGYFMSADGIKFRKPV
                     FPGDTIFIHAELTKARGNRLAKAKCQCVVNDAVVSEGELMFTFLER"
     gene            26453..27640
                     /locus_tag="VFO30_02240"
     CDS             26453..27640
                     /locus_tag="VFO30_02240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9418134.1"
                     /translation="MSLRLWLLLLLVITIAVRTPTMFGVKRTGWDERAYVVFAQTLDK
                     VGVSGIRQWLHEYPINEQLQKSPLFLRVGFIVPAMLTCKLLGDFNADNLAWLSFCCGV
                     AVILLGARFADDLGGRKMSILCGILLVSSPLATALSRRGTQDSFAALLTLACLYLFDR
                     CWRRRSVLDLIGLAFCLCLAFLTKETTLLLYPLMGIAAAYYFRAMELRASPWLLVPLI
                     TAPIVYFMVEIGICGGIGNFIGTYRLYASVQQTLDYTVHYEKGPWFRYLLDFLAIAPL
                     SFIAAIVGFSVPHNAALRHGRNLALIYFAGAVLLFGQMPIVNVRLLLFADMFLRFAAA
                     AGITYLATKIASAWSARVLYLGIAFVVMTDAFQFYRVFVAGNVYSPTTFLLLRAEGFY
                     QVQ"
     gene            27667..28446
                     /gene="lpxA"
                     /locus_tag="VFO30_02245"
     CDS             27667..28446
                     /gene="lpxA"
                     /locus_tag="VFO30_02245"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET9418135.1"
                     /translation="MTDVQIHATAIVDVTAELGTGTVVGPYCVIGANVVLGPECWLQH
                     QVTLIGPMRAGSGNKFYAYCSIGQQTQDLKYAGEPTYLEIGDENTFREFVTVNRSTTS
                     EGKTRIGSGGNFLAYSHIGHDCVVGDGVVFSNNGTLAGHVEIGDHAVMGGLTAVHQFC
                     RIGRFAITGGCSKIVQDVPPFMIADGNPAKIRGVNLVGLERKNYAPEAVKVIKEAFRL
                     IYRSKYNTQQAIEAIRNELPASQEVQQIIEFIQTTQRGIIR"
     gene            complement(28483..30018)
                     /locus_tag="VFO30_02250"
     CDS             complement(28483..30018)
                     /locus_tag="VFO30_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HET9418136.1"
                     /translation="MKRRCCLLLMLIIVPDARAEQSLAESTIVLYNKNVPDSVQLAKF
                     YADQRGIPRDHLVGLDCPQEEEISRDEYDRTIANPLREIFKTRNWWILRGSPGENATI
                     TATSIRFVAVIKGVPLKVRATTNYPGDVPGQPPIANRNEASVDSELAVLGLSSRQISG
                     ERSNPYFQNFRAIGDFDNAVLLLVCRLDGPSPDVVRRMITDAIATEKSGLWGRAYVDG
                     AHNTTGDFAMGDEWLRSIADQLHNVGVPVIYDDKPETFPNGFPITDAALYYGWYNGNV
                     SGPFARPDFHFLPGAIAVHIHSFSAATLRDANANWVAPLIAKGAAASIGNVYEPYLQL
                     TPHLDIFNDRLLHGFTFAESAYMAVRVLSWMTVMVGDPLYRPFASWLQLESTTADTQW
                     KRYHEFAVKNAARPPSEFRSLAWQTASAERNCPMLEDLAAIEAAQGDFSAATAHFPQA
                     RRCYRARDDLLRVTMEEVDAWRKQNSPKRALELVRTALRNAPDAPAAPLLRKMEAELA
                     AAK"
     gene            complement(30019..30741)
                     /locus_tag="VFO30_02255"
     CDS             complement(30019..30741)
                     /locus_tag="VFO30_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9418137.1"
                     /translation="MIGCDCDVCRSSDPRDQRTRSSIYVETPESAFVVDTGTDFRTQA
                     LRENIRRVDAVIFTHSHTDHIMGFDDLRRFSYARGSMPVYASPETMRDLERVFEFAFK
                     GTYSIPGYLKPEPYVIDGPFKLGGTLVTPLPVPHGDSTINGYLFSRGGEKLVAYLSDC
                     SRVPDAIVDLVSGVSALIIDALRDKPHPTHLSVGQALEVASHVRPGATYFTHICHELP
                     QSDEAWLPVNTSIAYDGLKLRF"
     gene            30897..>32111
                     /locus_tag="VFO30_02260"
     CDS             30897..>32111
                     /locus_tag="VFO30_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9418138.1"
                     /translation="MPAVLNLLRIKNLALVEELQWQMPAGFIAVTGETGAGKSIIIGA
                     LQLLLGERADKTLIRTGAEMCTVEAVFAGDGVDKLNLRLIESGIDPCGDELILKRTLS
                     TSGANRQFINGSPTTLSILKQLGDELVDLHGPHDHQSLLSPEKQLQLLDSFARAQEQL
                     DEYRKHYRQLQTLLAEHAALNTAETAREQELDLLRHQISEITSANLVADEEEEIENRY
                     KLASNSKRLIELASSAANKLSEADDSVLSQLADTQRLLRELEKIDNSVAQFSAAHAAS
                     VVELLEIARALSNYAEKLDLDPVQLAALEQRVSLFETLKRKYGGSIADVIAFAERAAE
                     RMRKIEGRDEELERLAKELENVRAQLSRAGDALRKLRSTAAPKLSTTIRSNLVDLGFR
                     QAEFEAKLFALDE"
BASE COUNT         6938 a         9405 c         9324 g         6444 t
ORIGIN      
        1 gaaagatgcg gccgagcaat cccggcagga aaaccagcgg gataaacacc gccgcgagcg
       61 acagtgtcat cgacaggatc gtgaacgaga tctcgccggc ggtgttgtaa gcagctcgca
      121 caatcgactc accatgctcc gaccggcgga cgacattttc caaaaagacg atcgcatcat
      181 cgacaaggaa gccgatggcg agggtgagcg ccatgagcgt gagattgttg atcgagaaat
      241 tgagcacgta catgaccgcg aaggtcagca ggagcgacag cggtagcgcg acgatcggaa
      301 tgagcgtgtc agtggcgcga ccgaggaaaa cgtaaatcac catcaccacg agcatgaacg
      361 cgatcacgag ggtgaagcgg acatcgtgca cggaattgac gatcgtctgc gaccgatcaa
      421 ataccggcac aagcgtgatc gagccgggca aactcgcgag cagttcgggg aaaagcgctt
      481 tcactgaatt ggcgacttcg actgcgttcg cgcccgcctg ccgtgaaacg gcgagcacga
      541 ctaccgagcc cggcggatgg aatccccgcg cccaaaacgt ccgcgacatg cgttcgtcct
      601 gcacactctg gcgcacatcc gcgatgtcgc gcaaatggac gggcgaaccg tctcgatttt
      661 ttgcgacaat caaattgcga taaccatccg cgtcgtcgat ttggccattt ggctgcagca
      721 caaacgttcg gtgggcgccg tcaaattgcc cggcgccgga gtaaaccgtg ccggctttga
      781 tcgcgctggc gaggtcatcc atcgtgagac cgcgagcggc caatgccgcg ggatcggcct
      841 tgattcgaat cgccccctga acgccgtaga tgttcacctg cgagacgccc ggcaggatcg
      901 agatgcgctg agccacggcg gtcgacgcgt acttgtataa atcgccgtcg gtcagtgtgt
      961 ctgacgtgag accgagcaag taaaccgcct ggtcgttcgg gttcgttttc gtgaacgtcg
     1021 gtgggctcgg taggtcgatc ggcaatttgc ccgtggcccg ctgaatggct gcctgcacgt
     1081 cagtcgccgc gtcagtgatg ctcttgctca ggttgaactg aagagtgagc gacgtgttgc
     1141 tctgcgaact cgcactcgtg atcaggtcga ggccgggaat ttggagaaat tgtttctcga
     1201 gcggtgtagc gatgttgttc gccatcgttt ccggactggc gccgggatag gaacaggtca
     1261 cttgaatgat cgggtaatcg acggccggaa ggtcgttgac cggcagcttg ttgtaggtcg
     1321 cgaggccggc aacgatgacc gcgagggtca gcaaaacggt catcaccgga cgctggataa
     1381 acggaccgga caatccagag ccggtgactt gaatcagctt ggcgtcggga gttttcgacg
     1441 gcggcgcggc cgcttgggat ctggtttgat cctcgccgtg ccgtagctcg ggggatcgat
     1501 cttgtgttct gtgcgacggc tcgtgttcgc catcggcagc cccattctct tcggcgattc
     1561 gatccattta tttaaagcgc gctcttcgtt gcactcgagt cactagtagg cccgaccggg
     1621 ccattgtacg gttttggatc cacctttgtt cccgggccaa gtgcaagctg accagtgaca
     1681 acaacggctt cattcggctt cacgccgctt tcaatgaccg tgagatcagc gtcctggcgc
     1741 tgcccgggtt taacgggccg caattcgaca gtgtcgtcag gcttcaccac aaatacgtac
     1801 ggaccacttt gactgatctg caccgcttgc gagggaacca acgttgcatc tttgatcgtg
     1861 tccagaatga aacgaacacg gacaaattcc gatggccaca gcgcgcgatc cggattcggc
     1921 gtgaccgcgc gcgctttcac cgttccggaa ccgggctgga ctgcgttgtc gatgaaatac
     1981 agatcgccaa gtcgcggcgc aatgttggcg tccggcgacc aggtttgcac tttcaggttc
     2041 ggactgccga gatatttgcg gaccaaggcg agatcagtct ccgcgacggt gaaatcagtg
     2101 tagatcgggt cgagaccctg gatggtgacg agcggcggac tggacgggcc aaccaagttg
     2161 ccgacgtcga cattgcgcag acctattcgt ccgttgatgg gcgattttat ataacagaaa
     2221 tcgagattaa cctgagcagc cgcggctatt gcttcggact tgcttgcgtt cgcctgggca
     2281 gtgtcgagat cttggggcga gacgacatta cgggcgcgca gttgctgttg ccgcttcagc
     2341 gtagcttggt ccaatgcgac ctgagcgcgg gcctggtcga gcgcggcttg atacgggcgc
     2401 ggatcaatcg tgaagaggag atcgccttgc ttcacctcgg ctccgtcctg aaaatggcgc
     2461 gccatgatct ggccgctgac ctgcgcctgc acctgcacgg tctcgtacgc ggcgcacgtg
     2521 ccaatctcat ccagatacag cggcacatct ttggtcgtca cctgcgcgac gagcaccggt
     2581 cgcttcggtg gttgcgccgc tttaccctct tgcgcgcgat gctgaacaac gcggtagaga
     2641 gcgaaggccg ccaagaccaa aaagagaagg taagcccaac cgggaatagt ctggctcctc
     2701 gcgagttgac gcttcattta gcacctcccg cgctgggcca gcgttcccgt tgttttgtaa
     2761 tgcgctggcc gttgctcttt tcgccgttgc cggccgacgc cccggctccg agcccgtttg
     2821 gccgcgcctt gtcacgcttc tctgctcgcg cgcggatacg ggtcagcaca tcgatgcagg
     2881 tctgcaattc tgatgccgcg acatcggcca gcaattcatg gcgaagctca tccgcggcgg
     2941 cgttgatgtg cgagatgacg ctttgcgcgc gtcgacccaa cagcacgagc ttgcagcgcc
     3001 tatcatgcgc ggcattggca cgttttaccc agccttctcg ctccaaacgg tgtagtaatg
     3061 tgacgagcgt cggctcttcg attccgagtc gctctgcaat ttctgtttgc gttagcgcct
     3121 ccggtgcgat cgacaggtgg agcaaggtcc gccacttcgc ctgactcaag cccatcggct
     3181 ttaaccgctc gtccagcttc aatcgccagg cgcgcgcggt gccgtgcagc aatggaccga
     3241 atatttccgg atcaatctgg ctcattgcga gaatagttag catcctaact atctcgcgct
     3301 tgtcaaacag ccgtgaccgc gcctcaggaa ctctccaccg cgaacaaaat caccgtttcg
     3361 taccggtaat cggtgaatcg cagcactggc ctcgcacgac gcggccgcca ttgttggctg
     3421 tttgaagcgc tctcctcatc ctccggcaga acaagaaagt aatgcgtgtc cggcggaagt
     3481 tcagccacac tgctcacata tctaacggca gcatcgacat agaaaagaaa tggctgatag
     3541 ttcgggtcga cggcgtacaa aagctcgttg cgcggaacga ttgagttgat ctgctcggcc
     3601 atatttttca ccttggggcg atgtttaaaa atcatcgccg cggcgagtgg atagccaacc
     3661 agaactgccg cggccacaaa cgcgacaagc ggctgcccaa cgcgtgacca cagcagctca
     3721 ggcggttgct tcagccacgg caacggcgca aacgcgtgct cggcgaagct tagcccggtc
     3781 agaagacaaa acggcaccag cacgggcagg caatagcgcg gtgccatgcc tggagtgaga
     3841 ttaatgagca ggaagggaac cactatactc cacgcgagtg cccgcgccag gtcgcgctgc
     3901 ctggcttcgg cgaaccggcg cagtcgtagc cacgggacaa agactaacca gggcagaaaa
     3961 taaatgagcg atcgcgaaag catttcgaac cagttcgcga gccgcaggaa ctctcccgtc
     4021 acccggccag cgtattgccc gagccatttg cgcatgatta gcggactaga ttccgtttgc
     4081 agaaaaggga cggcccagag gccgaacact gcaagcatga tgagccagcc gacgaggtgg
     4141 gccgggtgaa tcaggaaccg ccactgtttg tcgcgccaga gtaccgccac aactaccgca
     4201 taaaagaaga acagaagggt cggacccttg gccagccagc caaggcccag aaaaatccac
     4261 ggcacggtcc aggtcagcca aggtgagcgt ttttgttccc accaactcag ccagcagatg
     4321 atcgcgagtg cgcagagcga aatgtaaagc gcttcgattt caatgagtcg gcccttctcg
     4381 atcatgccga aattcgtgag ccagacgagc gccgcgatcg tcgcaccgcg cgaaccaaca
     4441 ctgccgcgcg cgacggtgag aaacgccagc gccacggcta agacgcaaac gacagaaggc
     4501 gcacgcgccg tccattcgtt gcgatgtccg aaaagtttga atgagaccgc caccaaccag
     4561 ttcactaagg gcggctttcg gtaataagcc gccccgccga ctgtcgggac caaataattg
     4621 ccgctttcga gcatcgccac ggcaggcaaa atgcgccgcc cttcctcgcc tttgatttcg
     4681 aacgagccga ggcccggcaa ataaatcgcc gcccaagcga caacgacgag caaccatgat
     4741 ggcaggcgcg acatgcgctg attgtcccga agaggccgtg gcgtgcaacg gctattcgtt
     4801 ggttctggat cccacagtgc ggatgattgt cgattttctc gaccgcggat cgtagcgccg
     4861 ttacgggcgg gcgccgtaag taagccgaac ctgcgggcaa tcgcaccttg acggaacgac
     4921 ctcggaaatt agattaaggc tctagtccgc gctgattaga gtgggaactg gtgtcccact
     4981 ctttttagtc tgcggactgg atgttttatg aacgccgaac tcatcgcgat gctcgattac
     5041 ctcgagcggg agcgcggaat caaacgcgat atcctgctcg aagcggtctc gaacgccctg
     5101 ctctcggctt cgaaaaaaag cgtcagcgtc tcacgtgatc ttcgcatcga cattaatcca
     5161 acaacgggcg tgatccgcgc actggccaac ccgctcgtgg tggagaccgt gagcaatccg
     5221 caggacgaga tctccctcgc caaagcgcgc ctgattaatc cgaacgtaaa ggtcggcgac
     5281 tcagtcgaga tggaagtaac accgcgaaat ttcggccgca tcgctgctca aacggccaag
     5341 caagcgatga tgcagcgtat tcgccaggtg gagaaggaga tgatctacga ggaattcaag
     5401 gatcgtgccg gtgaaatcgt tagcggcacc gtgcgtcgct tcgatcgctc tgacgtgatt
     5461 ctcgatctcg gaaagttcga agccatcatg ccgcagcggg aacgcgtcgt ggtggaagat
     5521 tacaatatcg gcgatcgcct ccgcgcctac gtcgtggcgg tggacaacgg catccgcggc
     5581 ccggagatca tcgtttcgcg cagtcacccg aatttcgtta ggcgactctt tgaactagaa
     5641 gtgagcgaga ttgccgacgg taccgttgaa atccgcggca ttgcgcgcga ggccggctat
     5701 cgcacgaaga tcgcggtctg gagtgcgaat gaaaaggtgg atcccgtcgg cgcgtgtgta
     5761 ggcatgcgcg gttcgcgcgt gaaaaacatc gtgcgcgagc tcaacaacga aaaagtggat
     5821 atcatccgct ggagctccga ccccaaggaa tacattctcg aagcgctcaa accggcgaag
     5881 gtaaagaacc tcctcttcga caccgaaaag aaaagcgtcc agatctccgt ggacgaagat
     5941 cagctttcgc tcgccatcgg caagaaaggt caaaacgcgc ggttaacgtc ccgattgacc
     6001 ggttgggaaa tcaacatcga caaagacact tcggccacca ccgcggtcga gcaaaaggtt
     6061 gcccaagccg cccagaccct cgcgggtgct ttgccggtca cccaagaaca agctgtcacg
     6121 ctcgtgaaat ccgggttcac gaatctggag ggattgcgtc atgccgacgt ggaagatctg
     6181 gtcgatattc tcggtgttga ccaggcaaag gcgcgcgaaa tacacgaggc ggtaaaccga
     6241 gaaggtgcgg aggaaaaagt tggctcagcc caaagcgctt gagccatgaa ccagtttttg
     6301 caaaacagca atgccaacca agtcgatcaa aaaaccggca acgcggaaaa ccgccgcgtc
     6361 gaaaacggta aaggctgctg tttcgaagcc cgcagctaaa tcagccgcac caaagaaagt
     6421 cgaacgcaaa ccggcgcgac cagatcgtgc ggccagggcc gagaatgcgg gcgccgcgcc
     6481 actgccggaa gcaccagcga agccagcgga accgctaacg ggtcgtcatg aggtaaagag
     6541 tgtttcgctc attgacgccg aaagacccga gaacaagtcg gacaaagacg gtcagttaaa
     6601 gaccaagagc acaattctcc cgccgatttc ccgcatccgc gcgtcgttcg agacgcccac
     6661 ggttcccccg gtgccgaaga tcgacaagat cgcgccgcca gaaactgcgc ggcccgcaga
     6721 actaacgacc gagccgacgc cagtagtacc cgatggaatc gcggctccgc cagagccagc
     6781 accggaagaa gaggcgcaaa aggtcattca tataaagccg ccgatcatcg tgaagcagtt
     6841 ggcgacagag ctcggtctca agccgcatca actcatagcc gagctgatga cgttcaacat
     6901 tttcgccaat attaatcaga caatcgagcc cgacatcgcc gcgaaaattg ctgagaacca
     6961 cggattcgtt ctcgagaagg aacgccgcga aaaaggtggc ggcgtgcaca aggtcgagca
     7021 ggtcgtcgtc gcgccgcccc cgccgcttat tacgccagag gaagaactca agtcgcgtgg
     7081 cccgatcatc actttcatgg ggcatgtcga tcacggaaag acttcgctgc tggatgcgat
     7141 ccgtaaaacg cgtgttgccg cgggtgaggt gggcggcatt acccagcaca tcggcgcgta
     7201 ccaggtcgat tacaaaggca ccaagatcac cttcatcgac actcccggtc acgccgcgtt
     7261 cactgcgatg cgcgcgcgag gcgcgaacgt caccgacatc gtcgtgctcg tcgtcgcggc
     7321 ggacgacggc atcatgccac aaacagtcga ggcgattaat cacgcgaaag ccgctccgca
     7381 cgtcaaaata atggtcgcca tcaataagat cgatttgccc ggcgcaaaca tcgacaaggt
     7441 gaaaaagcag ctgcaggagc acggcctcac accggaagac tggggcggtg aaacaatcgt
     7501 ctgcccggtg tcggcgacga aaggcacagg catcgatcac ctgctcgaga tgatgacgct
     7561 gcaagccgaa gtgatggagc tgaaggcctc ccctactgcg aaaccgcgcg gcaccgtcat
     7621 tgaagcccaa gtcgaacccg gccgcggacc caccgcgagc gtgatcgtgc aaatgggcac
     7681 gttgaaaatc ggcgacccgt tcatctgcgg cgattacgcc ggtaaaatca aatcgctcat
     7741 ggacgatcgc ggtcagcctg tgcgcgcagc tgggccggcg acgccggtaa aagtggttgg
     7801 cttcaccggt ctaccaaacg caggcgacga attcctcgtg atggattcgg aacgcgcggc
     7861 caaaacgctc agcgaggagc gtctcgaagc gaagcgcgcc aataaactcg ccgtcccgca
     7921 acgcgccacg ctcgagagtt tgcttgaagg accagccggc aaaaagacgc tgcgcattgt
     7981 gctgaaatgc gacgtgcaag gctcggtcga agcgattgtc ggcgcgttgc agcacatcga
     8041 gagcaagaaa gtcgatctgg aaatcattca ctccgctgtt ggcccgattt ccgagtcgga
     8101 cgttctgctc gcgagcgcct cgaacgcggt cgtcgtaggg ttcaatgtca aggtcgaaag
     8161 catggccgtc ccggcggcga aacgcgaagg cgttcaggtc aagctttaca gcatcattta
     8221 tgagctaatc gaccagatca aagaagcgat ggctggactg ctcgaacccg agcaccgcga
     8281 gaccattatt ggccatgccg aggtcaaaca ggttttccaa ctcagcagag gcatcgtcgc
     8341 gggttgtctc gtcaccgacg gccgggtttc gcgttccgct cgggcgcgtg tgctccgaaa
     8401 acgtcagccg gtttacgacg gcggcatttc tacgctgcgc cggttccagg atgacgtgaa
     8461 agaagtccgc agcggtctcg agtgcggcgt taagctcggt gatttcagcg aataccaggt
     8521 cggcgacatc atcgaatgtt accagctcga agctgtcgca cagaagttat gaaacatcgg
     8581 caagtacgcg taaacgaagt ggtaaagcgc gagctcagcg gtatcattgc tcgagagatc
     8641 aggtttgagg acgcgctcgt cagcatcaac gaagtggacg tagcggccga cctaaaatca
     8701 gcgcacgttt ttgtgagcgt gctcggaacg gccaccggcg cgagcgtgat tgagcagctc
     8761 gaggagcatc gtcctgacct gcagacggcc ctcgctcggc gcgtcgtgct gaaatacaca
     8821 ccgcacctag tttttcattt ggacgaatcg attcagcgag gtacgcgtgt catccaaatt
     8881 ttacaagata tggaagcggc tgcgggcgaa aacgagtgag cgccgttaac gcgagcttca
     8941 aggagatcgc ccgcgccatt tgtcaacacc agcgcatcgc tgttctcagt cacgtccggc
     9001 cggatggcga tgcgctgggc agccaaatcg cgctcgccct ctcgttacaa caaatcggca
     9061 aagatgtgcg ggtctggaac gaggacggca tgctcgaaaa atattctttt gtcccgcgcg
     9121 ccgagctcgt gaccaaacca ccggccgaac ccgaagatgt cgatcttgcg atcgcgctcg
     9181 acaccgccat tcagaaccgg ctgggcaccg cgctcaccgc gattcgtgca acgaaacttt
     9241 ggattaacat tgaccatcat ctcagcaacc cgcgttacgg cgatctcgtc cacatcgatg
     9301 acagcgcccc agcgaccgga caaattctgt ttgatttgat aacgagcgaa aaacttccga
     9361 tcgatcggga catcgccgaa aacctttacg tcgcgatctc aaccgacacc ggatcgttcc
     9421 agtatccaaa aaccaccagc cgaacgtttg aaatcgccgc cgagttagtt cgcgtcgggc
     9481 tcgatgttgg tgcgatcagc acgcagctgt acgaaaactt tccgcgtcgc cgggtcgaac
     9541 tcttgcgcga gctcctgcgc acgatgcgct ttgacgggaa cggtcgtgtc gccagtttca
     9601 agctgtccct gcaaacggca aacgagcttg gcgtcattcc cgaagacaac gagggactaa
     9661 tcgaccatct gcgcgcgatt cgcggcgtca tcgtcgccgt ctttttcgag gaactgcccg
     9721 acggaaaaat tcgcgtaagc atgcgctcca aagacgaacg aatcgatgtc tgcgcgatct
     9781 gtcagaaatt cggcggtggt ggtcacacgc tcgccgccgg tgcgcgcatt cgcggcacct
     9841 tgtcggaagt cgaggagcgc gttttacaag ccgcttgtga cgccgtcgac cgccaatcct
     9901 gacggcgtcc tgctcgtcga caaagccgaa ggcatgacgt cgcacgacgt ggtcgcactc
     9961 gttcggcgca agctggagat gaagaagatc gggcattgcg gaacactcga tccgatcgca
    10021 acggggttgc ttctcctcac gctcggccgc ggaaccaaag tgcaggacct gttgatgagt
    10081 gaggataagg aatatgtcgg cacactcacc ctcggcatca tcaccagcac gcaggatcga
    10141 caaggtgaaa ttgtcgacca gcggccggtg ccggcgctaa cagacgatca aatccgtgcc
    10201 gcgttcgaaa aatttcgcgg cgacttctac caaatgccgc cgatggtctc ggccaagaaa
    10261 cacggcggcg ttccgctcta caaactggcg cggcagggaa aagtgatcga gcgcgagccg
    10321 cgcctcgttc acgtgtatcg ctacacaatc gatcacgtcg cgcttcccga cattgatttc
    10381 agtgtgcttt gcagcaaggg cttttacgtg cgcacctatg cacacgacat cggcgagaca
    10441 ctcagttgcg gcgctcacct gaagtcgctg cgtcgcacga aatccggccg gtttactgtc
    10501 gatcaagcga ttaccatcca cgaaatcaaa aactcgccgg tcgaacagat caccaaccga
    10561 attctgactc tccctgaggt ttcgcgaatg cgcggcgcat aagcggaaag atcgacacga
    10621 tggaaattct gcagtcaatc tcccaactgg aaaagctccg cggtccagtt tttctcgcca
    10681 tcggggtttt tgacggggtc caccgtgggc accaggcggt gatttctact tcggcaagtc
    10741 acgcgcaagc cggcggcacc gcggtcgtgg tcacattcga tccgcatcca gctaaggtgc
    10801 tgcgcccgca cgacgcgccg catctgctca ccgcgacaga gcacaaaatc gcgctcattc
    10861 gcgaattggg tgttgaacac ctgctgctga tcaaattcga ccggcaattc gcctctaccg
    10921 cgccgaaaga tttcatcgcg cagctcgtgc gccattccaa tccgctgcgc gaaatctgtg
    10981 tcggccatga atggtcattt ggaaaggacc ggcgcggaaa tgtcgatctt ctaagaaagc
    11041 tcggggctca atacggcttc gaggtggtca gcattccgcc cgtgaaagtg aacggcacgg
    11101 tggtgagcag cactgcaatc cgacaggcga tcgaaattgg cgatttcgcg aaagccgcgc
    11161 ggatgcttgg gcgcgattac acgatccttg gcaccgtgac cagcggtgaa cgcctcggca
    11221 aaaaacttgg gtttccgacg gcgaacctaa gcgcccatag cgaacagttc ccgccgaatg
    11281 gtgtttattg cgccgaggcg aaactcgatg ggcaactgtt acgcggcgtc gtgaatcttg
    11341 gctacaggcc aaccgtcagc gccgcgaagg acgagcgcgt cctcgaaatc cacctgctcg
    11401 atttcaaccg cgacgtttac ggcaaggaca tcgaggtccg gttcacccgt catctcagac
    11461 cggaaaagaa attcgaaagc gtcgatgcgc ttgtgcgaca gatcgagatg gatgttaagc
    11521 aagcacgcga actgtgcacc ggctgatccc ggcgccctga ttcaaggcca cagccgcgcg
    11581 acgtcgcgca cctctggcgt tttgcgcagc gcaaagatca cggcgaaggc gccaaccagc
    11641 aggccgcagc cgaacacgaa aacaagcaac agccaaatcg gaaagctcga caactggatt
    11701 atgccttcgc tgacgaagcg gccgccgccg gagagcaagt aggcgatcgc gcttccaagt
    11761 aaggtgagcc cgatgaggat aatcgcgcgg agttgccatg gctcaggtcg ggccgcgggc
    11821 agttttgcca tgatacgcgc cgtgaatcca tcatcatcaa tgtacggccc tgcttcacgc
    11881 agttgtcgat ctaacgtctc gtcgtcgatc atcccgttca ttgtatcaat ttggccccca
    11941 ggccgccagc gtgcgtttca acttctcgcg cccgcgcagg acgttcgttt tcactgtgcc
    12001 gagagggatg tccaggacgc gcgcggcttc gtcgtgcgat aaaccgtttt ggcagcaaag
    12061 caccaccgcc gtgcgctcat gcagtggaag taagctcagt gcgtgcatca gatcgtggcg
    12121 caatgcggga tcgacagttt gcgggtcgcg ctcccgctcc agctgttttt cgtcaacacc
    12181 gaccagctcc cggcgtttgc gcgcgtactc gcgaaagcag ttgtaggcaa tgcggtatag
    12241 ccacgtggaa aaacgcgctt caccgcgaaa gctgcggagg tttttgtagg cacgcaggaa
    12301 ggtctcctgc gccagatcgt cggccagact aatgtcatgt ccagtaagct ggcgaagcag
    12361 accgcgcacg gtggattgat gattgcggac aagctcggca aacgcgtgat gatcatcgtc
    12421 aactaagact ctggcaacca gctctgcatc tgtgagtggc acccgcgcaa attatggcaa
    12481 gggcggtggg ttgtctggtc tgcgtttcgc gcccccctca agcttccaca caatcaggta
    12541 accggcgccg atgagaaacg ggatcaagcc gagtgtccac gctccgcctt cccaatcact
    12601 gaccgccgcc aaccatagca tcaggcctac gccgagcatc accaggataa ccccccggcg
    12661 catatcagag cgttgacgag ctggcagttt tccaggggcg agcaattcgg ccggcaccgg
    12721 ttgacctttt tcgaccatca tttgaatcgt ccgatgcaac cgccgatttt ttgaaatcgc
    12781 aacatagccg agtaccgcaa tcatcaggac cggcgcgcca aaaacggcca gcatactaat
    12841 tccgacaatt ggaaagacct cccctggaac gtcttcgtgg tcgtggcgat ccagctcatc
    12901 ggtgtcaatc attactccat ggcggccgcc gaatttttcc tcgattcggc gctgaatttt
    12961 ctcggccagg tcgcttgcgg gtgaagcggt cacactcggc gaagcggcaa ccgaagggga
    13021 aagtgcaggc gagcccggct gcggcgtcgc tggaggttgc gcgaacacca ccgtcgcgag
    13081 cgcggcgatg ccgattgaga cagagagtaa ggttttcata atcgtccttt gcctgtgaga
    13141 tgcggaccag gtgcaacttg gattcacaaa agcacgcttt cgctggacaa ttgcagggca
    13201 ggctccgcct gctgacgtga tggtaagcga tgcgcttgca gtgcaattca gcacacccgc
    13261 tcgtcgtgac gcaagagaca atcgctatgt cagcctttaa cgaattgctc ctcttcggtg
    13321 gagccgcgca atgcggccgt cgagcattcg cccccgcaga tgatctgcgc gacggcatcg
    13381 aagtagccaa cacccacctc gtgttgatgc ttcgtcgccg tgtagccgaa ttcctcagcc
    13441 gcgaattccg cttcctgcaa ctcggcatag gcggccatcc cgcgagcggc gtagccacgc
    13501 gccagattaa acatcgaata attgagcgta tgaaaaccag ccagcgtaac gaactggaac
    13561 ttgtatccca ttgcggccag ctcgcgctgg aacttcgcaa tcgcggcgcg gtcaagcttc
    13621 ttcttccagt tgaatgacgg cgaacaattg taagcgagca acttgttcgg gaaccgctcg
    13681 cgcacgggtt cggcaaaccg acgcgcttca tcgagattcg gtgcgctcgt ctcgcaccag
    13741 accagatcgg catagggcgc gtagttgatc gcgcgcgcca ccgcttgttc caaaccggcg
    13801 cgtacacaga aaaatccctc cggcgttcgt tcgccagtca ggaactgatg atcgcgctcg
    13861 tcaacatcgt tggtgagcag ttttgcgcca ttcgcgtcag tccgcgcgat gataatcgtc
    13921 gggacattag agatgtcggc agccaaacgc gcggcgatca aattgcggat ggcatgtgat
    13981 gttggaatga gcaccttgcc gcccatgtgc ccgcatttct tctcgctcgc gagctggtct
    14041 tcgagatgca ccccggccgc tcccgcttcg atcattgcct tgatcagctc gaacacgttg
    14101 agcggtccac caaagcccgc ttccgcgtcg gccacgatcg gcaccagcca gtcgatgcct
    14161 accgcgccgt agccaggcgg aggtgggtcg atcttgtccg ccggacggac ccgctgcggc
    14221 gggccttcgg cgtggtcgat ctggtcggcg cgcagcaaag cattgttaat acgccgaatc
    14281 accgccggga cgctgtttgc cggatacaaa ctctggtctg gatacatttg cccggcgaga
    14341 tttgcatccg ccgccacctg ccagccgctc aggtaaatcg ctttcagtcc ggcgcgaacc
    14401 tgctggacag cttggttgcc agtcagcgcc ccgagtgcgg gaataaaatc tttcgtctga
    14461 agtaaattcc acagcttttc tgcgccccgc cgcgccagtg tatgctcaat cgcgaccgtg
    14521 ccgcgcaagc gctcgacctc tgccgccgaa tacggccgct cgattccgtt gaatcggcca
    14581 tccttatccg cgcgccgctg cactcggttt cgtttgccgg cgggccgctc tccgtctcga
    14641 tgcgctttca tctcaatcca tgtactcgta gcccggcaag gtcagaaact cggcgaattc
    14701 gtcatcggtg gtgatctttg caaacaattc gcgtgctaag gtgtatttgc gcttctcgaa
    14761 gatggattgg cctaactgat ttcgaacctt gccaagctcc tcatccagca cgtgcgcgaa
    14821 cagttccttc gtcactttgc ggccgtcggt cagctttccg tcggggtggc gaatccattg
    14881 ccaaacttgc gcgcgtgaga tttcggaggt cgcggcgtcc tccatcaaat tgaaaagcgg
    14941 tacggcgcca tgaccgcgca gccacgcctc gagatattgc acgcctacgg aaacattctg
    15001 ccgcagcccg gcttcggtga tgggctctcg cggcgcgaag tcgagcagat catccgccgt
    15061 cacatttacg tggtcgcgtt ttcggtcgat ttggttaggc gttttcatca cggcattgaa
    15121 cgcgtcagta gcgagctgca ccatgcccgg atgcgcgacc cacgtgccgt cgtgaccgtc
    15181 gcccgcttcg cgttctttgt ctgcgcgcac tttggcgaac gcctcctcgt tcgctttcgg
    15241 gtcatccttc accgggattt gcgccgccat tccgcccatc gcgaacgtgt tccgccgatg
    15301 gcatgttttg atgcagagca gcgaatagga atgcatgaag tgcgtcgtca tcgtgacaag
    15361 cgcgcggtcc gccaggatga aatccttttt cgcgcggaat tttttgatgc aggaaaaaat
    15421 gtaatcccag cggccgcagt tcaggccggc ggaatggtcg cgcagttcgt aaaggatctc
    15481 gtccatctca aacgccgccg ggatcgtttc gatcaaaacg gtcgcacgaa tcgtgccgcg
    15541 cgggataccg agctcgtcct gcgcgagatt gaacacgtcg ttccaaatcc gcgcctcgac
    15601 atggttctcc atcttcggca ggtagaagta aggtcccgtt ccgcgctcga gcagctcgcg
    15661 cgcgttgtga aaaaagaaca agccgaaatc aaacaatccg ccagagaccg gcgcaagatc
    15721 gacaactacg tgtttctcat cgaggtgcca gccgcgcggc ctaacgagca gcaccgcgag
    15781 ctcgttcttc agcttgtaat ccttcccctc cggactgcga aaagtgatcg tccgccggat
    15841 tgcgtcacgg agattgatct gaccttcgac catgttccgc cacgtcggcg aattggcgtc
    15901 ttcgaaatcg gccatgtaca tcttcgcgcc ggagttcagc gcgttgatta ccattttgcg
    15961 atcggtcggc ccggtgatct caacgcggcg atcgcgcagg tcgtttggaa tcagcgcgca
    16021 agtccagtcg ctttcccgaa tgtgctctgt cgcagacaga aaatccggca tctcgccagc
    16081 atcgaaacgc aattgtcgat cttgccgcgc cttcagcgct tccttgcgac gcgcggcgaa
    16141 ttcgcgatgc aacttcgcca caaacgccag cgcctccggc gtcagaacct cggcgaactc
    16201 gggcgtgatc tcccccttga tttcaatgcc ccggggcaaa gtcgattgca tcgcctttta
    16261 ttcggtatgc gccgggcgat tggccgcaaa cgcccggtcg aaaagccttg cgcaaccggc
    16321 caagtcgcac attatttcac tccctccagc ctccgctaaa gctacggctg gcaggcaacg
    16381 cgcgggtgta gctcagtggt agagcacctc cttgccaagg aggacgtcgc gagttcgacc
    16441 ctcgtcaccc gctccagcct ttgcttggag cccagcgtaa agcgaaggct ggagcgtccg
    16501 ccgtagcctt ctgcgaaggt ggacatccat cgcctccaag ctacggctgg caagccagtc
    16561 tgtgatgcaa tgtattacgt ttgcctctta cgatcagaac accacccgaa ggaacaatac
    16621 atcggccttg ccgctaacct cagagagcgg ctacgcgatc acaatcttgg taattcgttg
    16681 tatacttcga aattccggcc ctggcttctg gtggcctatt tcgcctttgc aaacaaagag
    16741 cgagctgttg cgttcgagcg atatttaaag tccggctcag ggagagaatt cgcgaaacgg
    16801 catttcatct gaaaatgtca cctacgtgat cgcttcgcga atgccgtgca attgctctac
    16861 tagccgatga tattcgtcaa tccttagtgt ctgttcgccg tcggaaagcg cggattccgg
    16921 acgcggatgc acttcaatca tgacgccgtc cgctccggcc gcaacaccgg cgcgcgccag
    16981 cggactgacc atgtaattgt ggccggtccc gtgcgccgca tccacgatca gtggcaaatg
    17041 cgagattcga cgcatcgctg gaattgcagc gagatcgagc gtgttgcgcg catgttgcga
    17101 gaaggtgcgc acgccgcgct cgcacaacac gatttggtta ttgccttccg acatcacgta
    17161 ttcggccgca agcagccatt cctcgagtgt cgccgccatt cctcgtttta gaagcaccgg
    17221 cagccgcgaa cggccggccc ggcgcagcag cgtgaaattt tgcatgttgc gcgagccgat
    17281 ctggatcatg tcggcgtatt gctcgacaag gccgaccgtg gattcgtcca gcgcttcggt
    17341 cacaactcgc agcccgaatt tcgcgcgaac ctctgcaaga atctttaagc cttcttcgcc
    17401 gagcccggca aaggagtaag gcgaagtccg cggcttaaat gcgccgccgc ggaaaaattg
    17461 cgcgccactg cgctgcactg cttctccaac cgcgaaggcc tgttctcgat tttcgatggc
    17521 acatggccca gcgatgatcg tgagcgaacc ggcgccgatt tgagcgccct taacatcgac
    17581 aactgtgcgc tccggccgcg catccaacga ggttaacttg aacggattcg tgacgcggat
    17641 cgcttccgtc acgccgggaa gattcgcaaa acgtgcggtg tcgagcggct cgccatttcc
    17701 ggtcaggccg accgctgtcc gggacgcgcc cggcatgacg tgcgcgtgac agccgagcga
    17761 ctcggccacc cgcaccacat tgtcaatttc cgccggcgcg gcgttggcat tcatcacaat
    17821 gagcatggcg acaggaactg atacttactt ggacggatgc cccgcggtca actttaacgc
    17881 gcgaggacat tgacgggcgg gagaatgcgc tcaatctcgg cgcggtgcgc ctcgtattgc
    17941 ggcggtagct tcaggctccc gccgaggtgc tccaccggct cgtcggctgt aaacccaggc
    18001 ccatcggtcg cgatctcaaa caaaatgccg gctggctcgc ggaaatagat cgagtggaaa
    18061 tattggcgat cgatcactgg cgtaatgtac agcccaagat cgacaatccg tttgcgccac
    18121 tccaactgct cggcttcgtc cgcacaacga aacgcgatgt ggtgcacagt acctgcggaa
    18181 ttcacgccga acgacccgtc cggtcgttcc atcaaatcca ccgccgcact tgtcgatggt
    18241 tcatcaactg agaaccgtcg ccgcgagtta tcttcggcga caatttcgaa cccgaacgtc
    18301 tcgagcagat tggcagtttt cgtcgcgtgt tgcacctcca gcgtcgccgc atggaaaccg
    18361 tgaatcgcga actcgttggg cacatcgctt ttgtagccaa gatcgacatc ctccagctct
    18421 ggtgacgtga ttagctcgat cagcaaacca tcaggatcag cgagtcgtag cacattgtcg
    18481 ccgaaccgcg ggctcgtttg ctcgcacaac acccgctgtt cttcgaagcg cgctttccaa
    18541 taatcgagcg cgctctgcgg aattgcgaaa ctcgtcgcga ccacttgccc cacgccgcgt
    18601 gtgccgcgcc gcgctccagg ccacggaaaa aatgtgatca ctgtccccgg tcgcccggtg
    18661 cgatcgccga agtagaagtg ataggttgaa ggatcatcaa aattgaccgt gcgcttcacc
    18721 aagcggagtc cgagcacgtt tgtgtagaag tccaggttcc gctgcggctc agtcgcgatg
    18781 gcggtgatat ggtggatgcc gcgcagcttc attcgatctt ccagtatgta cctctggcga
    18841 cgccaagcga cttcgcaatg gtggcgagtg catcgaagcg cgctggaatc gcgcttcggt
    18901 gacaagctcg cggtcatcgc ccagccgggc cgcaagcgat tagaaatcca gattgcagtc
    18961 aattcgatga gcagattgcg cgaactggtg aaaatcgccg acggccgaat cgaaaaactc
    19021 ccgcgggact ggctggaacg ttttcagcgt cgggacaaac caaaactgat cgggatcaaa
    19081 gatcgacaat tgatcattcc cgccggcgcg gcgtttggga ccggtgagca cgccaccacc
    19141 gcgatgtctc tccacatgct cgaaagactg gcgcagactt gtagtgacgg ctggtcaagt
    19201 atcgatcttg gcactggttc gggaattctc gcgctcgcgg cgcgtctgtt cggcgcgaag
    19261 cccgtgattg gaatcgacaa cgatccgagg gcggtttcga cggcaaagca gaatgcgcgg
    19321 ctgaacaaga tcgacaatgt tgggttcaga gccgcggacg tgcgctcgtg ccaactcccg
    19381 cgcaacatcg acatcatcac ggcgaacttg tttagcgaat tgctcgtcga aattttgccg
    19441 aaattgcgcc gtgccgcgtg gcttatcctc tccggtattt tgcgtgagca ggagcgtgag
    19501 ctcatccatg cgctcaagcg gcaaaagatc aacattgtcc aggtgcgacg ccgcgggaaa
    19561 tgggtcacga ttctcgcgcg cggattgcga cggtcataga ccgccgctac agtgaaaatc
    19621 ggcatgcaaa gcgatgagct cgcctatgcc gttgtcacgc catactcgat gcgcaaatcg
    19681 cgcaccggcg ggatcgttgc gcgcctgatt tcgcgcaccg gcctcgatct cgtcgccgct
    19741 cgcatgttcg cgccgagcaa ggaactgacc gagcgctacg caaaaacaat cgtcaccgag
    19801 accgagccgg cgcatcgcgc cacccaggag ctgatccgcg attatgtgct aaaaaatttt
    19861 accggtgaga aaaatggcca ccggccccgt gccttgtgcc tggtttttcg cggtccggat
    19921 gccatcgaaa agattcaccg cgcggtcgga cacatcgtgc acgagcgcac gagcggcgag
    19981 acgattcgcg acacgttcgg cgattacatc accgatgatt cgggccgggt gacgtatttt
    20041 gagccagggg tgctcgcggc tttcgatccc agcgccgtgg cgcgcgattt aaaactctgg
    20101 gctgagtttt ccgatcgcga cggcggcgtg ctcgatcaag cagtgcagtt cgccccggga
    20161 acgaagatcg agaagacgct cgttctcatc aagccggaca atttcaagtt cccgaacctg
    20221 cgacctggcg gcgtgatcga agttttctcg cgcaccggtc tctacatcgt tgcgttcaaa
    20281 gtgcaccgga tgagtgtcgc gcaagcggag gaattttacg gtccggtgtt accggagttg
    20341 caggagaaac tcgggcccgc gaaaggacgc agcgcgtggg aagacatcgt tgcgttcatg
    20401 gccggggccc gaccaagcga cacgttaccg gagaaccgag aaaaacctgg cacagagaag
    20461 tgtattgccg ttgtttacca gggcgaaaat gccgtcgcga aaattcgcga ggtgctcggc
    20521 ccgaccgacc cggcgaaggc gccgccgggg tcgatcagaa aagagttcgg ccaaaatatc
    20581 atggttaacg cggcgcacgc gtccgactcg gtcgcgaacg cgaagcgcga gatgacaatc
    20641 gtgcgcgtcg aggaaaacaa ctttaaacct ttgattgaaa acttttatcg ccgccaataa
    20701 ggtgcgctcc ctgaatttcg aacgctaaga tcgacatgga aatttccgaa cgcgccgggc
    20761 aactgactcc ttcgcttacg ttgtcgatcg acagcaaagc gaaggcgatg aaagccgaag
    20821 gcattgacgt ctgcggcttc ggcgcaggcg agccggattt cgacacgccc gagcacatca
    20881 aagccgccgc gatcgcggca ttgcaggccg gttttaccaa atacacgccg agttccggga
    20941 ttccggaatt gcgtcaggcc atcgcggaaa aattggcggc ggacaacggc ctgacctatc
    21001 gcgccacgca agtggtggta agcaacggcg cgaagcacgc ttgttacaac gccatcctcg
    21061 cgacgtcgca gcccggcgat gaagtcatca ttccgtcccc ctactgggtg agttatcccg
    21121 acatggtgag gttggccggt gccgagccgg tgatcgtgcc gacgttcgag cggaatgcct
    21181 ggaaaatgcg cgcggaagat ttcgagaatg cgatgacccc gcgcaccaag atgttgatcc
    21241 tcaatagccc gtccaatccg gccggctccg tttacactcg cgaggagttg caggcgattg
    21301 tcgatgtcgc agccgaggaa gacatttacg ttttgtccga cgaaatttac gagaagctca
    21361 tttacgacaa cgcgcagcac gtcagcatcg ggtcgctgtc gaaagaagcc tacgacctca
    21421 ccatcacggt caacggcttc agcaaatcgt acgcgatgac cggctggcgg ctcggttacc
    21481 ttgccgcgcc ggatgcagtc gcgaaagccg tcgagtccat ccagagccac agcacctcga
    21541 acccgtcgtc attttcacag cacggcgcgc tcgccgcgct gaaaggcgac cagcaaccgc
    21601 tcgccgacat gcgcgaagaa ttcgatatgc gccgcaacta catgtacgac cgcatttcaa
    21661 aaatcccgaa cgtcaccgcg gtcaaaccgc agggcgcgtt ttacgtgctg gtgaacgtga
    21721 gccagctcgg cctcacctcg caaaattttg ccgatcgttt gctgagcaaa gcaaatgtcg
    21781 cggtcgtccc cggcgccgca tttggcgatg atcggcttgt ccgcctgagt tacgcgacga
    21841 gcatcgacgt catcaaaaag ggtctcgacc gtttccagga cttctgccgg acgctctaat
    21901 ttgtatgtca tccggaggtc gccgggggcg gccgagggac ctcacagtcg tacgggctat
    21961 cgcactaatt tcgctgaggc gatgcgcagc ttgctgcaac gagcgtggct tcttacctga
    22021 tccaggcgct tttgagaggt tcctcgcttc gctcggaatg acacgctaat tgtttttaat
    22081 gcctgggcac gtttcaacgg acaaagccgc tgttgtcgcg cgcatcaaag ccgagggggc
    22141 ggtggcggtt attcgcaccg attcacctga gcgagcgctc gccgcggcgc acgctgtcgt
    22201 tgcgggcgga tttcgtgcgg tcgaaattac ctacagcttc gctggcgcca ccgacgtgat
    22261 cgccaaactg gccgaggcga acgagaacaa tttgctcgtt ggtgcgggaa cgattttgag
    22321 ccgcgagcag gtgcacgaag ctgttggagc aggcgcgcgc ttccttgtca gcccatgcat
    22381 gctgccggac gtgattgacg tcgcacgcga gcacgaggtt gcagtcgtgc cgggcgcgtt
    22441 cacgcccacc gagatcttca cagcgtattc gttaggcgcc gatatcgtca aaatttttcc
    22501 tgctgttcgg tttggtccgg aatatttgcg agccgtgcga ggtccgctgc cgcacattcc
    22561 cattatgccg acgagcgggg tcgaggcatg caacgtcgcc gagtggttca gggccggtgc
    22621 agttgctgtc ggtgccgttg gcagcgtgct ggatcctata ctgattcgaa acggcgagtg
    22681 ggatgcgcta acgaaccgcg cccgtgaatt catcgccgcg gtacgtgccg cccgaacagg
    22741 ggatcaggtt gcgaacggca ctcgcagcgc gaaaactgcg cggtgagcaa aggccatctc
    22801 gcgctcattc tgcacgccca tctaccgttc atcaggcatc ctgagcacga gcatttccta
    22861 gaggaagaat ggttcttcga ggcggccacc gaaagctata tccccctgct gcaaatgcag
    22921 cagcggctgg taaacgacgc cgtgccgttc aagctcacca tatctctgac gccgacgctc
    22981 accgccatgt tgcaggacga gctgttacga gaacgttacg tgcaacatgt cgatcttttg
    23041 atcgacctcg ccgcgcgtga gcaaaagcgc aatcgcaaag acgcgcgcct gagagaactc
    23101 gcgcattttt attcccggtt cctttccgcg acgcggcggt tttttgtcga cgaatgcaag
    23161 tgcgatttgc tcgcagcatt tcgaaaactt cgtcgagccg gtgtcctgga aatcatcgcc
    23221 tcggccgcga cccatggatt gctcccgttg ctcgcgcagc aatcacccgc agcggcacgc
    23281 gcgcaggtgc tcatcggccg tgatcaatat gtcggtcttt ggagtgaaga accgaaagga
    23341 ttttggctgc cggaatgcgc atatgcgccg gaacttgatc gaattttgca agagacaaac
    23401 atccggtggt tcgtgctcga cgcgcacggt ctgcttttcg gcaatccgca gccgtgtcgc
    23461 gcgattttcg caccttgtta tacgcgtcgc ggcccggccg cattcgcccg cgaccgcgat
    23521 tcgagtcggc agatctggag tgcgcatgag ggctaccccg gcgacccggc ctaccgcgaa
    23581 ttctatcgcg acgccgggtt cgatcttccg ctcgagcatc tgggaccggt cgccgccggc
    23641 cgccgcaaat tttcggggct gaagtaccat cgcatcaccg ggcgcggccc ggagaaggaa
    23701 ttgtacgatc gcgcggcggc ggagcacaga gcccagactc acgccgcaca ctttctcgag
    23761 caacgacgcg aacaacttcg ccggttgaac caacaaagcg ccgatccgat cgtcgtcatc
    23821 ccgttcgatg ccgagttatt tgggcattgg tggttcgaag ggccgctgtt ccttgaatgg
    23881 ttcattcgca aaaccgcgtc tgagcaggat gacttttgcc tgacgacacc gagcgagtac
    23941 ctggccgcgc atccgaccca gcaaacgatc gagccggcaa gatcgacatg gggcgagaac
    24001 ggcgatctta gcgtctggat taatccggcg aacgcttgga tttatccgca tctgcatggc
    24061 gcagcgcgtc gcatgaccga agttgctcgt gcgcatgcca atgaaccgtc atcgctcggc
    24121 gaccgcgttt tgaagcaact cacgcgcgaa ttgttgctcg cgcagtcaag tgactgggcg
    24181 tttttgatga aaaccggaac ggcacgcgag tatgcgacca aacgcacgat cgatcatctc
    24241 ggccgattta atcgcttgca cgaccagttt gcagcaaaac gaagcgatga agtgtttctg
    24301 cgcgattgcg aagagcgcga caatttgttc ccggatgtga actggcgcta ctatgcctga
    24361 attgcagatc gcaaattgca ggttgcaaat tgttgccgtg tggctgctcg taattggtgc
    24421 ggcagcgcaa gagtcgtcga cgccaccaat atcggttacc ccgactccac cgccctcaag
    24481 cgcgcgcact gtccgcatta gctttgttcc gcctccgctc gaagggacga tcagcctcgg
    24541 cattttcgat cgcaccggga agctcgtgcg cgttttgcat caagaggccg aattggacga
    24601 gttcacagtc gaggccgacg gcctggagac gaaatgggat ggtaaaaatg acgacggcaa
    24661 aggtttaccc gcagggaaat accgtgcgcg cggttacacg gtcggctgca aggtggagga
    24721 gcttggaact tcccctaacg gattaccgag caacacaacc gagcatgtga cggtgaagct
    24781 cgttgccaat cctctgtcaa agggtgcgaa cttggttgtc gaacttgcag ttggattcga
    24841 cgatgaaaat tgcttccttc aaacaaccga tggtttgccg ctgttgacgg tgagcgaaac
    24901 gccgaacctg tggcgcgctt ttgtcgcgaa gagcggcgag cgttcagtcg acgtgtttca
    24961 ggacgacggc gacactatcg accagcttcg cgtttcgaat gtgaatcaga tgatggcgtt
    25021 cgattgcggc gagatcgagc tgaagtgaat tgtaacggcg gcggtgtcag ccgccgaaat
    25081 taaaagaacg gcgcttgaca caagcgccgc tacaacgcaa gcgagattaa ccacggcgaa
    25141 tgacaaccga agcgcaacaa acattagcta agccggcggg gtttagcggc acgtcgctac
    25201 acacgggcga gaaggtcgcg ctaaaacttc agccggcgcc ggtcgatcac gggatcaagt
    25261 tcaagcgcaa agatctcgcg gaagaaccgc tcatcgacgc gaaaatcgac aatctgaaaa
    25321 cggtcgagcg cgctaccaca atcggggaag gcgcagtacg ggtgcacacg gtcgaacacg
    25381 tgctcgctgc gctgtccggg atgggcatcg acaacgcgat catcgagatg gattcaaatg
    25441 agccgccgat cggtgatggc agcgcgcagc catatgtcga tgtaatcaaa agagccggca
    25501 ttacctcaca ggatgcgccg cgcaaatttt tcgaatcgcg cgagccggtg cacatcgaat
    25561 cgaaaaccgg cgcgctgctt gttcttctgc ccgatgaaaa attccgcgta tcctgcaccc
    25621 aggccggccc gaacaaccgg ttcacccaat ttctgtcgat ggaaataacg ccggctctgt
    25681 tcgaacgcga gatcgcgccc gcgcgcacct ttgtttacta cgaggacgtt cgtgctttga
    25741 tggacaaaaa cctgatcaaa ggcggcagcc tcgagaacgc tattgtcatc cgcgacaacg
    25801 ccgttctcgg caaagagccg ctccgttttg aggacgaatt cgttaggcac aaaatactcg
    25861 acatagttgg cgatctcgcg ctcctcgggc ggcgtgtccg cggtcatctc attgcggtca
    25921 agcccggtca tgcggtcaat gccgacctgg cccgcgtact cgcgcgcgag caggcgcgtc
    25981 gcagtgagat ggaagtgccc cgcgcgatgc cgcgcggcga tggcggactt ggcacagacg
    26041 aaatcatgca ggtattgccg caccgctatc cgttcttgat ggtcgatcgg gtggtcgagt
    26101 ttcagggcga caacaagatt accggcgtca aatcggtctc aatcaacgag ccgtttttcc
    26161 agggacattt tcccggtcac cccgtcatgc cgggggtgtt gctggtggaa gcgatggcgc
    26221 aggtcgccag cattctattg ttcaaactcg caaaaacctc gagtcgcatt ggctacttca
    26281 tgagtgccga cggaatcaaa tttcgcaagc cggtgtttcc gggtgacacc attttcattc
    26341 acgccgagtt gaccaaagcg cgcgggaacc gcctcgcgaa agcgaagtgt cagtgtgtcg
    26401 tgaacgacgc cgtcgtttcg gaaggcgaac tgatgttcac ctttctcgag cgatgagtct
    26461 gaggctttgg ctgctactgc tgctggtgat aacaattgca gtccgcacgc cgacaatgtt
    26521 cggtgtcaag cgcactggct gggatgagcg cgcttatgtc gtgttcgcgc aaacactcga
    26581 taaggtcggt gtctccggca ttcggcagtg gctgcacgag tatccgataa acgaacagtt
    26641 gcagaaatct ccgctgttcc tccgggtcgg attcatcgtt ccggcaatgc tgacgtgcaa
    26701 actcctgggc gacttcaatg ccgataacct cgcgtggctc tcgttttgtt gcggcgtggc
    26761 tgttattctc ctcggcgccc gctttgccga cgatctcggt ggccgcaaga tgtcgatctt
    26821 atgcggaatt ctcctcgtta gttcgccgct tgcgaccgca ctttcccgcc gcggcacgca
    26881 ggatagcttt gctgccctgc taacgctggc atgtctctac ttgttcgacc gatgctggcg
    26941 gcgccgctca gtcctcgacc tgatcggcct cgccttttgc ctttgcctcg ctttcctgac
    27001 gaaggagacg acgctgctgc tctatccact tatgggcatc gccgcggcct actattttcg
    27061 cgcaatggag ttgcgcgctt cgccgtggct gctcgtgccc ttgattacgg cgccaatcgt
    27121 ttacttcatg gttgaaatcg gcatctgcgg cggcattggg aacttcatcg ggacatatcg
    27181 cctgtatgcg tcggtccaac agactctcga ctacacggtg cattacgaaa agggcccatg
    27241 gtttcgttat ctgctggatt tcctggctat cgcgccactg agcttcatcg cagcaatcgt
    27301 cggcttctct gttccgcaca acgctgcgct tcggcacggg cgaaatcttg ctctcattta
    27361 tttcgccggt gccgtgcttc tcttcggcca aatgccgatt gtcaatgtgc ggcttctcct
    27421 tttcgccgac atgttcctcc gattcgcagc ggcagcaggg atcacatacc tggcgacgaa
    27481 aatcgcatca gcgtggtcgg ctcgcgtctt atatcttgga attgcttttg tcgtgatgac
    27541 tgacgcgttt cagttctacc gcgtcttcgt tgccggcaat gtctacagtc cgacgacatt
    27601 tttactgctg cgcgcggaag gcttttatca agtacagtaa attatccgac aagcagaccc
    27661 caaactatga ccgatgtgca gattcatgcg acggcgattg tcgatgtgac ggccgagctc
    27721 ggcacgggca ccgtcgttgg tccatattgc gtcatcggcg cgaacgtggt gctcgggcca
    27781 gagtgctggc tgcagcatca agtcacgctt attggcccga tgcgcgccgg cagcggaaac
    27841 aagttctatg cttattgctc gatcggccag cagacacagg acctgaaata cgcgggtgag
    27901 ccgacgtatt tagagattgg tgacgagaac acgtttcgcg agttcgtcac cgtgaatcgc
    27961 agcacgacga gcgaaggcaa aacgcgcatc ggcagcggcg ggaattttct tgcttacagt
    28021 cacattggtc acgattgcgt agtgggtgac ggcgtcgtgt tctcaaacaa cggaacgctc
    28081 gctggtcacg tcgagatagg tgatcacgca gtcatgggtg gtctcacggc agtgcatcaa
    28141 ttttgccgga tcgggcgatt cgctatcacg ggcggctgct caaagatcgt gcaggatgtg
    28201 ccgccgttta tgattgccga cggtaatccg gcgaagattc gcggtgtgaa cctcgtcggc
    28261 ttagagcgaa aaaattatgc gcctgaagcc gtgaaggtga tcaaggaggc gtttcgcctg
    28321 atctaccgtt caaagtacaa cacacagcag gcaatcgagg ccatccgcaa tgaactgccg
    28381 gcgagccaag aagttcagca gatcatcgag ttcattcaga ccactcagcg cgggatcatc
    28441 cgctaaaact cgagttgtca tcctgagtgc catcgaaacc gactatttcg ccgccgcaag
    28501 ctctgcctcc attttccgca ggagtggcgc ggccggcgca tccggggcgt ttcgcaaagc
    28561 ggtgcgcacc agttccagcg cgcgcttggg actgttctgc ttcctccacg catcgacttc
    28621 ttccatcgtg acgcgcagga gatcgtcgcg ggcacgataa cagcgtcgcg cctgtggaaa
    28681 atgtgcggtc gctgccgaga aatctccttg ggccgcttcg attgccgcta aatcttccag
    28741 cataggacaa ttccgctccg ctgatgcggt ttgccacgcc agcgaacgga attcagatgg
    28801 aggacgggcc gcatttttga ccgcgaattc gtggtagcgc ttccactgcg tgtcggccgt
    28861 ggttgactca agttgcagcc acgacgcgaa cggccggtaa agcgggtcgc ccaccatcac
    28921 cgtcatccag gaaagcacgc gcacggccat gtaggcgctc tcggcaaaag tgaagccgtg
    28981 cagcagccgg tcgttaaaga tgtcgaggtg cggtgtcagt tggaggtatg gctcgtaaac
    29041 gttgccgatg cttgcggccg cgccttttgc gattaatggc gcaacccaat tcgcgttcgc
    29101 atcgcgcagc gtggcggcgc tgaacgaatg gatatgaaca gcgatcgcgc ccggtaaaaa
    29161 atggaaatca ggccgcgcga acggtcctga cacgttgccg ttgtaccagc cgtaatacaa
    29221 cgccgcatcc gttatcggaa agccgttggg aaaagtttcc ggcttatcat cgtaaatgac
    29281 cggaactcca acattatgca gctgatcagc aatcgagcgg agccattcgt cgcccatcgc
    29341 gaagtcgccg gttgtgttgt gggcaccatc gacgtaagcg cgcccccaca gtccgctttt
    29401 ctctgtcgca atggcgtcgg tgatcatgcg ccgcacgaca tctggactag gtccatcaag
    29461 tcggcagacg aggagtaaca ccgcgttgtc gaagtcgccg attgcgcgga aattttggaa
    29521 atagggattc gatcgctcgc cagagatttg ccgcgaggag aggccaagca ccgcgagctc
    29581 ggagtcgacc gaagcttcgt tgcgattggc gatgggcggc tggcccggca catcgccggg
    29641 gtaattggtc gtggcccgaa cctttagcgg aacaccttta atgacagcga cgaagcggat
    29701 tgatgtggca gtgatcgttg cgttttcgcc gggcgatccg cgcaggatcc accaattgcg
    29761 cgtcttaaaa atctcgcgca gcggattcgc gatcgtcctg tcgtactcat cgcgactaat
    29821 ctcttcctct tgcggacaat cgaggccgac gagatgatcg cgtggaatgc cacgctggtc
    29881 ggcgtaaaac ttcgccaact ggaccgaatc aggaacgttt ttgttataaa gcacgatcgt
    29941 tgattcggcg agcgattgct cggcccgtgc atcgggcacg atgatgagca tgagcagtaa
    30001 gcaacatcgc cttttcatct agaagcgaag cttgagcccg tcataggcga tggaggtatt
    30061 caccggcagc caggcctcgt cggattgcgg taattcgtgg cagatatgtg taaagtacgt
    30121 cgcgccgggt ctgacgtgcg aagccacttc caaggcctgt ccaacactga ggtgcgttgg
    30181 gtggggtttg tcgcgcaatg catcgatgat cagcgcgctc acgccggata caaggtcgac
    30241 aattgcgtct ggaacacggc tgcaatcgct taagtaggca acgagctttt cgccaccgcg
    30301 cgagaagaga tagccattga tcgtcgagtc gccgtgcggc actggcagcg gggtaaccag
    30361 cgtgccgcca agcttaaagg gcccatcgat aacgtatggc tccggcttga gataacccgg
    30421 gatcgagtag gtgcctttga atgcaaattc gaatacccgc tcgaggtcgc gcatcgtttc
    30481 tggcgaggcg tagaccggca tcgagccgcg cgcgtaggag aaccgccgca gatcatcaaa
    30541 tcccatgatg tgatcggtgt gcgaatgcgt gaagatcacc gcgtcgactc ggcggatgtt
    30601 ttcgcgcagt gcttgagtgc ggaaatccgt cccggtgtcg accacgaacg cggattccgg
    30661 cgtctcgaca taaatcgacg agcgcgtccg ctgatcgcgc ggatcggaag atcggcatac
    30721 gtcgcagtcg cagccaatca tcggcacgcc ttgtgaggtg ccggttccca gaaatgtgag
    30781 tgtcagttga gagccttgct cgcgcagcct ttcggagttg agggttgaga gtggcgttgg
    30841 caagcttccc aagaccggaa catttatccc tcatccttca tccctcaacc tttcgcatgc
    30901 ctgccgttct caatctgctg cggataaaaa atctcgcctt ggtcgaggag cttcaatggc
    30961 agatgccggc cggcttcatc gcggtgaccg gcgagaccgg cgcgggcaaa tcgatcatca
    31021 tcggcgcact gcagcttttg ctcggcgaac gcgccgacaa aactctcatc cgcaccggcg
    31081 cggaaatgtg caccgtcgaa gccgtgttcg cgggagacgg tgtggacaag ttgaatctgc
    31141 gcctaattga atcgggcatc gacccgtgcg gcgacgagtt gattctgaaa cgcacattgt
    31201 cgacgagcgg tgcgaaccgc cagttcatca acggctcgcc caccacacta tcgatcttga
    31261 aacagcttgg tgatgaactc gtcgatctgc acgggccgca cgaccaccaa tcgttgctgt
    31321 cgccggagaa acagttgcag ttactcgatt ccttcgcgcg cgcgcaggag caacttgatg
    31381 agtaccggaa gcattaccgg caattacaga cgcttctcgc cgaacacgcc gcgctcaaca
    31441 cggccgagac cgcgcgcgaa caggagctcg atctgctgcg gcaccaaatc agcgagatta
    31501 catcggccaa cctcgtcgcc gacgaagaag aagaaatcga gaaccgctac aaactggcga
    31561 gtaacagcaa acgtctgatc gaacttgcca gctccgccgc gaacaaattg tccgaagccg
    31621 atgattccgt tctctcgcag cttgccgaca cgcaacgatt gctgcgtgag ctggaaaaga
    31681 tcgacaattc cgttgcgcaa ttctccgcgg cgcacgccgc gagcgtggtc gagctgttgg
    31741 aaattgcgcg tgcgctgtcc aactacgccg aaaagctcga cttggatccg gtgcaactcg
    31801 ccgcgctcga gcagcgcgtg tcgctctttg aaactctgaa gcgcaagtac ggcggctcga
    31861 tcgccgatgt gatcgcgttc gccgaacgcg ccgctgagcg gatgcgtaag atcgaggggc
    31921 gcgacgagga gctggagcgg ttggcgaagg aactcgagaa cgtgcgtgcg cagctgagtc
    31981 gtgccggcga tgcgttgcgc aagttacgat ccacggcggc gccaaagctg tccacgacga
    32041 ttcgaagtaa tcttgtcgat cttggatttc gccaagcgga gttcgaagcg aaactcttcg
    32101 cgctcgacga g
//
LOCUS       DASTDG010000071         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1145165, whole genome shotgun sequence.
ACCESSION   DASTDG010000071 DASTDG010000000
VERSION     DASTDG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145165"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFO30_02265"
     CDS             <1..735
                     /locus_tag="VFO30_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9418139.1"
                     /translation="EQAKEYIRAGDALGFYRCLRFINPSPYMFCLKLGDFALVGSSPE
                     MHVRLSGDLIDIRPIAGTRPRGATDAADARNAVELLADDKERAEHVMLVDLARNDIGR
                     VSEFGSVRVSEFMQIERYSHVMHIVSNVVGRLRRECTAFDLIKATFPAGTVSGAPKIR
                     AMQIISELEKTRRGCYAGAIGYIGFDGNLDSCIALRSAVLKNGKAYFQAGAGIVADSD
                     PRREYEETLSKAGAMAKALAMASGIT"
     gene            732..1796
                     /gene="trpD"
                     /locus_tag="VFO30_02270"
     CDS             732..1796
                     /gene="trpD"
                     /locus_tag="VFO30_02270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9418140.1"
                     /translation="MTTTMIESSSALRRITLRLINGEHLGRAEAGEFLDALLGPAATD
                     AQIAAALIALATKGETAEELAGMAEAMRERAIQINSRHTRFIDTAGTGSSAAKTFNVS
                     TAAAFVIAGAGLPVAKHGSRAATSRCGSADLLEMLGVNTAASPAIVERCLNEHGICFI
                     FAPLFHRPTARVARVRRELGVHTTFNLLGPLTNPAHPPFQVIGVWHHSLLEPVSSALA
                     LLGAKNAWVVNGSDGLDDVTLAGETFVAACRGNGATVKTLTIVPEEFGVARRSLDHIR
                     GGGPAENVQLVRAILGGEKNKEVAVARDLVIINAAAALHVAGVAHDLREAAALARDSI
                     DNGCALAKLDALVRETNRKS"
     gene            1793..2635
                     /gene="trpC"
                     /locus_tag="VFO30_02275"
     CDS             1793..2635
                     /gene="trpC"
                     /locus_tag="VFO30_02275"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9418141.1"
                     /translation="MSRDFLAEIIARKRRSVAAQRGRIDIEALRAQALQRRGNASAHR
                     LRQALRAASPEIKIIAEFKRRSPSAGAIRQAGTVGEMVRSYERGGAYAVSVLTDEEYF
                     GGSILDLAEARAAMQLPLLCKDFIVDRLQLYQAAAAGADAVLLIVAALSGDSLGELRA
                     TAENELALDALIEVHTLAELERAAEIGAAIIGVNNRDLHTFDVSLDVSERLINKAPTG
                     ALIVSESGLAEPAALHRLHALGFDGFLIGEALMRASDPEAALRKLLASAEDRQYPAVR
                     VSRP"
     gene            2632..3285
                     /locus_tag="VFO30_02280"
     CDS             2632..3285
                     /locus_tag="VFO30_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9418142.1"
                     /translation="MKQVQVKICGVTNMQDAHTCAELGADMIGLNFYRESPRYIEPEA
                     AREIVASVGKDVCPVAVFVEPALEHVRNLAQTVGLTTVQLHGDVSSEMCGELAREFRV
                     IRALCTHGEFRPHDAVQFRACDVLLDAAHPQLHGGTGTTCNWSAARATLPFTRFLILS
                     GGLNVQNVREAIAAVQPQAVDVCSGVELAPGAKDRAAVEQFIAAVRAVGVAELTRSA"
     gene            3289..>3932
                     /locus_tag="VFO30_02285"
     CDS             3289..>3932
                     /locus_tag="VFO30_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9418143.1"
                     /translation="MQRSTITRSEPDEQGHWGKYGGRYVPETLVAPLEELTAEFARAR
                     QDGEFWRELDEWRRDYCGRPTPLFHARRLTEHAGGGQIYLKREDLLHTGAHKINNAMG
                     QALLACRMGKRRVVAETGAGQHGVATATVCALFGLRCIVYMGAEDVRRQALNVFRMRL
                     LGAEVVTVNAGTRTLKDAINEALRDWVTNVHDTYYLLGSALGPHPYPLMVREFQ"
BASE COUNT          782 a         1122 c         1255 g          773 t
ORIGIN      
        1 gagcaggcaa aggaatacat ccgcgccggc gacgcgctgg gtttctatcg ctgtcttcgc
       61 ttcattaacc cttcgcctta tatgttctgc ctgaaattgg gtgacttcgc cctcgttggc
      121 agttcgccgg agatgcatgt ccggctctca ggtgatctga tcgacattcg accgattgcc
      181 ggcacgcggc cccgcggcgc gaccgatgcc gcagacgcgc gaaacgccgt cgaactgctg
      241 gcggacgaca aggaacgcgc cgagcacgtg atgcttgtcg atcttgcgcg caacgacatc
      301 gggcgcgtgt cagaattcgg cagtgtgcgt gtgagcgaat tcatgcagat cgagcgttac
      361 agccacgtga tgcacatcgt ctcgaacgtc gtcgggcgcc tgcgtcggga atgcacagcg
      421 ttcgatttga ttaaagcgac atttccagcg gggactgtgt ctggtgcgcc gaaaatccgc
      481 gccatgcaaa tcatcagcga actggagaaa acccggcgcg gctgttacgc cggagcgatc
      541 ggctatatcg gtttcgacgg taacctggat tcgtgcatcg cgctccgttc tgccgttttg
      601 aaaaacggaa aggcatattt ccaagccggc gcgggcatcg tggctgactc cgacccgcgg
      661 cgggaatacg aagaaaccct cagcaaagcg ggggcaatgg cgaaagcttt ggcaatggcg
      721 tcaggaatca catgactacc acgatgatcg agagctcatc tgcactgcgt cgcattacgt
      781 tacgtctcat caacggtgaa catctcggcc gcgcggaggc gggagaattt ctggacgcgc
      841 ttctcggtcc agcagccacc gacgcacaaa ttgcagcagc gcttattgcc ttggcaacga
      901 aaggtgaaac ggcagaagag ctcgccggca tggctgaggc aatgcgggag cgcgcgattc
      961 aaatcaactc gcgccacaca cgcttcatcg ataccgctgg caccggctca agcgcggcaa
     1021 agacgtttaa tgtttcgacc gcggcggcat ttgtcattgc cggcgctggt ctgccggtgg
     1081 cgaagcacgg ttcgcgcgcg gcaacatcgc gctgcggcag cgccgaccta ctcgagatgc
     1141 ttggggtgaa caccgccgcc tcgcccgcga ttgtcgaacg gtgtctgaac gagcacggca
     1201 tctgtttcat tttcgcaccg ctatttcaca ggccgacggc gcgcgtggcg cgggtgcgcc
     1261 gcgaactggg cgtgcatacg acgttcaatc tgctgggccc attgacgaat ccggcgcatc
     1321 caccatttca agtgatcgga gtgtggcatc actcgttgct cgagccggtc tcgtcggcgc
     1381 tcgccttatt gggcgcgaag aacgcctggg tcgtaaacgg cagcgatggc ctggatgatg
     1441 tcacactggc aggcgagacc ttcgtcgctg cttgccgcgg caatggcgca accgtcaaga
     1501 ccctcacgat cgtgccggaa gagttcggtg tagcgcgtcg atcgctcgat cacatccgcg
     1561 gcggtggacc ggctgaaaac gtgcagctgg tccgcgcaat tctcggcggg gagaaaaaca
     1621 aagaggtcgc ggtcgcgcgt gatctcgtaa tcattaacgc ggcggccgcg cttcacgttg
     1681 ccggagttgc gcacgatctg cgcgaggcgg cggcactggc tcgcgacagt atcgacaacg
     1741 gctgcgcatt agcgaagctg gatgcactgg tgcgggagac aaatcgaaaa tcgtgagcag
     1801 agattttctt gccgagatca tcgcgcgaaa gcggcgctca gtcgccgcgc agcgcggcag
     1861 gatcgacatc gaggcgctgc gggcgcaggc gctccagcgg cggggaaacg ccagcgctca
     1921 tcgtttgcgg caggcgttac gagcggcatc cccggaaatc aaaatcatcg ccgaattcaa
     1981 acgcaggtcg ccctccgccg gtgcaattcg ccaagctggg actgtgggcg agatggtgcg
     2041 gagctacgag cgcggtggcg cgtatgcggt gtccgttctg accgatgagg agtatttcgg
     2101 cggttcgatt ctcgatcttg ccgaggcgcg tgcggcgatg cagctcccgc ttttgtgcaa
     2161 agatttcatt gttgatcggc tccagcttta tcaagcagcc gccgctggcg cggacgcagt
     2221 tttgttgatt gtagcggcgc tgagtggcga ctcgttaggc gaattgcgag caactgctga
     2281 aaacgaactg gcgctcgatg cattgattga agtgcatacc ttggccgagc tcgaacgcgc
     2341 cgctgaaatc ggtgcagcca tcatcggcgt caacaatcgc gatctccaca cgttcgatgt
     2401 ctcgctcgac gtcagtgagc ggctcatcaa caaggctccg acaggcgcgt tgatcgtaag
     2461 cgaaagcgga ctcgctgaac cagcagcttt acatcgcctt cacgcacttg gcttcgatgg
     2521 ttttttaatt ggcgaagcat tgatgcgcgc ctcggatcca gaagccgcgc tgcgtaaatt
     2581 gctcgcctcg gccgaagatc gacaatatcc agcggttcga gtttcaagac catgaagcaa
     2641 gtgcaggtaa aaatttgcgg cgtcacaaac atgcaagatg cccacacctg cgccgagctc
     2701 ggcgccgaca tgatcggcct gaatttttat cgcgagagcc cgcgttacat cgaacctgaa
     2761 gccgcgcggg aaattgtggc gtcggtcggg aaggacgttt gtccggtcgc ggtgttcgtt
     2821 gagccggcac tcgaacatgt ccgcaacctt gcacagactg tcggcctcac gaccgttcaa
     2881 ttgcacggag atgtctcgtc ggaaatgtgc ggtgaactgg cgcgcgaatt tcgggtgatc
     2941 cgtgcacttt gcacacatgg cgagttccgg ccgcatgacg cagtgcagtt tcgagcttgc
     3001 gacgtgctct tggatgcggc tcatccgcag ttgcacggcg gcactggaac gacctgcaat
     3061 tggtcagcgg ctcgcgcaac gctgccattc acgcgttttc tgattttgtc gggcggtttg
     3121 aatgtccaaa acgtgcgcga ggcgatcgcg gcggttcaac cacaggcggt cgacgtttgc
     3181 agcggggtcg aacttgcgcc cggcgcgaag gatcgtgccg cagtggagca attcatcgcg
     3241 gccgttcgcg cggtcggagt tgcggagctc actcggtcgg cctaacgaat gcaaagatcg
     3301 acaatcacgc ggagcgagcc ggacgagcag ggacattggg gcaaatacgg cggccgttat
     3361 gttccggaaa cgttggttgc gcctctcgaa gaacttacgg ccgagttcgc gcgtgcgcga
     3421 caagacggcg aattctggcg ggaactggac gaatggcggc gcgattactg cggtcggccg
     3481 acgccgttgt ttcacgcgcg gcggctgacg gaacacgccg gcggcgggca aatttacctg
     3541 aagcgcgaag accttttgca caccggcgca cacaagatca acaacgccat gggccaggcg
     3601 ctgctcgcct gccgcatggg caaacgtcgc gtcgttgccg agaccggcgc ggggcagcac
     3661 ggcgtcgcga ctgcgacagt gtgcgcgctt ttcggcttgc gttgcattgt ttatatgggc
     3721 gcagaagatg tgcggcgtca ggcgctgaac gttttccgaa tgcggctgct cggcgcggaa
     3781 gtggtgaccg tgaacgccgg aacgcgcacg ctgaaggatg cgatcaatga agcgttgcgt
     3841 gattgggtta cgaacgtgca cgacacgtac tacctgctcg gcagtgcgct tggaccgcat
     3901 ccatatccgc tgatggtgcg cgaattccag ag
//
LOCUS       DASTDG010000072         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1076534, whole genome shotgun sequence.
ACCESSION   DASTDG010000072 DASTDG010000000
VERSION     DASTDG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076534"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFO30_02290"
     CDS             <1..908
                     /locus_tag="VFO30_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9418144.1"
                     /translation="RESARANTTIVIIKTAVVVFVIAFGAFMVHPTNWHPFVPNGFAG
                     VMSGAAIVFFAFIGFDAVSTTAEETRNPQRDMPIGIIVSLIICTLLYVLMSVVLTGIK
                     KYTVYTGDAAAVATAFAGRPWAQALVSAGALAGTTSVLLVFQLGQPRIFMAMARDGLL
                     PRYFAKIHPRFRTPYVTTIWTGVIVGGVAMVTNIGDLADLTNIGTLFAFILVCFGVNV
                     LRKVAAERPRPFRVPWVPVFPILGVVLCVALMLSLPVMTWIRFVVWLAIGLVIYFLYS
                     VRHSNLRRGLDVGPTEDIPPPIVKT"
     gene            complement(1017..1472)
                     /locus_tag="VFO30_02295"
     CDS             complement(1017..1472)
                     /locus_tag="VFO30_02295"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HET9418145.1"
                     /translation="MHKNVIGKDLTKEAPRSPRIRIGGYAILGRTIDKCRALVAGNIG
                     EYHFDCPLDNMLFGFKGVKGADFKAQIENGASDQQMAEWLDANGEKKTPGEIKRWGDE
                     VDATRPYDDPERRDWFVEQVKPLGLDPAKTTLFEWPEVDDKVSLAQKPA"
     gene            1559..2008
                     /locus_tag="VFO30_02300"
     CDS             1559..2008
                     /locus_tag="VFO30_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HET9418146.1"
                     /translation="MAQQKEIISTINSLIETLKDGQEGFKQAADAVKDPQLKSLFRDY
                     SQQRSRFVGELQSQARTLSESEPEKTSSAAGAMHRAWINIKSVVTSGDDHAILAECER
                     GEDSAVNEYKKAMEDELSLPLREIVSRQYSEIKSAHDRVKQLRDATK"
     gene            2052..3140
                     /locus_tag="VFO30_02305"
     CDS             2052..3140
                     /locus_tag="VFO30_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9418147.1"
                     /translation="MAPCYCLVRVTKMACRFWLLFCFIARLEAVDKIAPTPPGKLYHG
                     CFWDGSATDKHDPTEHDVTPGEVARYEQAVGKKTAWVYFSDNWFESRKFPSEMCGWIR
                     QLGKVPYVRLMLRSDLDQSHAERTFALKKIISGEFDLDLRTWAGEANAFGSPILIEWG
                     TEPNGDWFGWNGKWNGGARVGPQLYVAAYRHIVDLMRAQGADNLTWIWHVNWLDQPER
                     KWNRFENYFPGANYCDWLALSAYGPTTPTMRDGTESLRFKLGQAYPRLTSLAPGKPII
                     IAEFGCDLHNRHVDVVSWTKRALDDLFSGSWPAIIGFCWWNEGWQNDNRKKHDTDMII
                     LHNVDLTRVFRDEFAEHANKIQETPVVK"
     gene            3166..3615
                     /locus_tag="VFO30_02310"
     CDS             3166..3615
                     /locus_tag="VFO30_02310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9418148.1"
                     /translation="MISQAGGLRYIRPMVEVSIDYAGNMHCDATHGPSQSKLSTDAPT
                     DNKGKGEAFSPTDLVATALGTCMSTIMGMKAEELGLDIRGMKVSVKKEMSKDAPRRIV
                     ALPSEVHIPLPRDNPHREALEQAALNCPVHKSLPSEIERPTKFFWEG"
     gene            3646..4122
                     /locus_tag="VFO30_02315"
     CDS             3646..4122
                     /locus_tag="VFO30_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418149.1"
                     /translation="MRIRKAVEADIPKLLPLMRELAVFEKYDQAFAITEEVLREQGFR
                     RSPPDFHCLVADNETELAGFVVYYFVPFTYRATPNLVIKELYVMEAKRSQGVGELLMK
                     ATAQEAIRAGCGMIKWWVAKWNERGIAFYERLGAQIDSDWHEFQLSEAAIRDLAAS"
     gene            complement(4123..5193)
                     /locus_tag="VFO30_02320"
     CDS             complement(4123..5193)
                     /locus_tag="VFO30_02320"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HET9418150.1"
                     /translation="MNPKKSELLVEEIEHIDIKQFDVVDMVEVMSKMAFSARDLGRAA
                     HIYDAMLREKDCAIILCLAGSLISAGLKQVIVDLVRHNMVDAIVSTGAIIVDQDFFEA
                     LGFKHYKGDIFMNDDVLRRLAIDRIYDTYIDEDQLRICDNTCGEIADALEPRPYSSRE
                     FIREMGHYLTKNAKTRDSVVLAAFEQNLPIFCPAFSDCSAGFGLVAHQWNNPDRHVSI
                     DSAKDFLELTRLKIAAKTSGLFMIGGGVPKNFAQDTVVAADYLGFDVGMHKYAVQITV
                     ADVRDGALSGSTLREANSWGKVDQGSEQMVYAEATIALPLIAGYAWHKGAWKNREARN
                     FNKLLDEPAPAPSTQSKEPVAV"
     gene            complement(5255..>5638)
                     /locus_tag="VFO30_02325"
     CDS             complement(5255..>5638)
                     /locus_tag="VFO30_02325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9418151.1"
                     /translation="PASMVYHLAQQMRAGQRPRIFKHGEQKRDFVYVKDAVAATIGAL
                     EARESGIYNLGSGQARSFNELIDVLNNCLGTKFRPDYIENPHAHYQNFTEADLSQARR
                     ALGYEPRYPLEDGVRDYMQWLYPEK"
BASE COUNT         1183 a         1593 c         1637 g         1225 t
ORIGIN      
        1 ttagggaaag cgcgcgcgcg aacacaacga ttgtcatcat taaaacggca gtcgtagttt
       61 tcgtcatcgc gttcggcgcg ttcatggtgc atccgacgaa ctggcacccg ttcgtgccga
      121 acggttttgc cggagtaatg agcggagcgg cgatcgtttt ctttgccttc atcgggttcg
      181 acgccgtctc gaccacggca gaggaaacgc gcaatccgca gcgcgacatg cccatcggca
      241 tcatcgtgag cttaatcatc tgcaccctgc tgtatgtact gatgtcggtg gtgctgaccg
      301 gcataaaaaa atacaccgtg tatacgggag acgcggcggc cgtggcgacg gccttcgcgg
      361 gccggccgtg ggcacaagca ctcgtcagcg ccggggcgct ggccggcacg acttcggtgt
      421 tgctcgtttt tcaactcggc cagccacgca tcttcatggc gatggcgcgc gacggattgc
      481 tcccgcggta tttcgcaaaa attcacccac gcttccgcac gccctacgtc accaccattt
      541 ggacgggcgt gattgtcggc ggcgtggcga tggttacgaa catcggcgat ctggctgacc
      601 taacgaatat cgggacactc ttcgcattca ttctcgtctg tttcggcgtg aatgttttgc
      661 gcaaagtcgc ggccgaacgt ccccggccat ttcgcgttcc gtgggtgccg gtgtttccga
      721 ttctcggcgt tgttctttgc gtcgcgctaa tgcttagtct gccggtaatg acgtggattc
      781 gtttcgtcgt gtggctcgcg atcggactcg tgatttattt cctctacagc gttcgccaca
      841 gcaatctgcg acgcggtctc gacgtcggcc cgaccgaaga cattccgccg ccgatcgtta
      901 aaacttaaga tcgacatgtg cccggtggat cggccgctcc gcgggcgatg ttaaataatg
      961 cggcgttcgc cgccaaaata accatcgagc tcggagagct cgatccacct gagacgctat
     1021 gccggttttt gcgcgagcga gactttatca tcgacttccg gccattcgaa cagcgttgtc
     1081 ttcgccggat cgaggccgag cggcttcact tgttccacaa accaatcgcg ccgctcggga
     1141 tcgtcatatg gccgcgttgc gtcgacttca tctccccagc gtttgatttc cccgggcgtt
     1201 ttcttttcgc cattcgcgtc gagccattcc gccatttgct ggtcgctcgc gccattttcg
     1261 atctgcgctt tgaaatcggc cccttttacg cctttgaagc cgaagagcat gttgtcgagc
     1321 gggcaatcga aatggtattc gccgatattc ccggcgacaa gcgcgcggca tttatcgatt
     1381 gtccgcccga gaatggcgta gccgccaatc cgaatccgtg gactgcgcgg tgcttccttg
     1441 gtcaggtctt ttcctatgac gttcttgtgc atgattcagc ctagcgccaa atgttctgac
     1501 cgcaacggcg gtacgattcg caaacgcagc cggcaccctt cgcgcgaatt aattttatat
     1561 ggcacagcaa aaggaaatta tctcgacgat caacagcttg atcgaaaccc tcaaagacgg
     1621 tcaggaaggc ttcaaacaag ccgccgatgc agtcaaagac ccgcagttga agtcgctctt
     1681 ccgtgactat tcgcagcagc gctctcgctt cgtcggcgaa ctgcaaagtc aggcgcgcac
     1741 cctcagtgaa agcgagccgg agaaaaccag cagcgcggcc ggcgcgatgc accgagcctg
     1801 gatcaacatc aaatccgttg tcaccagtgg cgacgatcac gctattcttg ccgaatgcga
     1861 gcgtggcgaa gattccgcgg tcaacgaata caagaaagcg atggaagacg agctttcatt
     1921 gcccttgcgc gaaatcgtct cgcggcagta cagcgaaatc aaaagcgcgc atgatcgcgt
     1981 gaagcaattg cgcgacgcta cgaagtagcg cgcgccaatc gcaagatcga catctgcagg
     2041 gcgcggcgaa gatggcgccg tgctactgtc tcgtccgcgt gacgaagatg gcgtgtcgat
     2101 tttggctctt gttttgcttc atcgcgcgcc tcgaggcggt cgacaaaatc gcaccaacgc
     2161 cgccgggaaa actttatcac ggttgcttct gggacggcag cgcaacggac aaacacgacc
     2221 cgaccgagca cgatgtcacg ccgggcgagg tcgcgcgcta cgaacaggcc gtcggaaaga
     2281 aaaccgcgtg ggtctacttt tcggacaact ggttcgagtc gcgcaaattt cccagcgaga
     2341 tgtgcggctg gatccgccag cttggcaaag tgccatacgt ccgtttgatg ttgcggtccg
     2401 atctcgatca gagccacgcg gagagaactt ttgcgctgaa aaaaatcatt agcggcgagt
     2461 ttgacctcga tcttcggacg tgggcgggcg aggcaaacgc gtttggctcg cccattctca
     2521 tcgagtgggg cacggagccg aacggggatt ggttcggttg gaacgggaaa tggaacggcg
     2581 gcgcacgcgt cggcccgcag ctttacgtcg ctgcttaccg gcacattgtc gatcttatgc
     2641 gcgctcaggg ggccgacaat ttgacctgga tctggcatgt caactggctc gatcaaccgg
     2701 aacggaaatg gaaccgcttc gagaattatt tccccggcgc aaattattgt gactggcttg
     2761 cgctcagcgc ctatgggccg actacgccga cgatgcgcga cggcaccgag agtctgcgct
     2821 tcaaactggg ccaagcttat ccgcgcctaa cgagtctcgc gccgggcaag ccgatcatca
     2881 tcgctgagtt cggctgcgat ttgcacaacc ggcacgttga tgttgtgagt tggacgaaac
     2941 gggcgctcga cgatctgttc tccggctctt ggccggccat tattggcttc tgttggtgga
     3001 acgaaggctg gcaaaacgac aaccgaaaga aacacgacac cgatatgatc atcctgcaca
     3061 atgtcgatct tacacgcgta tttcgcgacg agttcgccga acacgccaac aaaattcagg
     3121 agacgccggt tgtaaagtaa cgcaagcgtc tcgcttgcaa tccttttgat ttcgcaagcc
     3181 ggaggcttgc gctacattcg cccgatggtc gaagtctcga tcgattacgc cggcaacatg
     3241 cattgcgatg cgacccacgg gccgtcgcaa tcgaagctat cgaccgacgc gccgaccgat
     3301 aacaaaggca agggcgaagc attctcgccg actgacctcg tcgcgaccgc gctcggcaca
     3361 tgcatgagca caattatggg catgaaagcc gaagagctcg gtctcgacat tcgcgggatg
     3421 aaagtgtcgg tgaaaaaaga gatgtcgaaa gacgcgccgc gccgcatcgt cgcattaccc
     3481 tcagaagttc acattccgct gccccgggac aacccgcacc gcgaagctct cgagcaagcc
     3541 gcgctcaatt gtcctgtgca caaaagcctg ccctctgaaa tcgagcgacc cacaaaattt
     3601 ttctgggaag gctaaagggc cgcgccaccc ggcgccggaa tcgacatgcg gatcaggaaa
     3661 gcagtcgaag ctgacattcc caaactactg ccgctgatgc gcgagctggc agtgtttgaa
     3721 aaatacgacc aggcgttcgc gattacggaa gaggttttgc gcgaacaggg attccggcgt
     3781 tcgccccccg atttccactg tctcgtggcg gacaatgaaa cggagttagc gggatttgtt
     3841 gtttattact ttgttccatt cacgtatcgg gcaacgccca atctcgtcat caaggaattg
     3901 tacgtgatgg aggcgaaacg cagtcagggc gtgggcgagt tgttgatgaa agcgaccgcg
     3961 caagaggcga ttcgcgccgg ctgcggcatg attaaatggt gggtggcgaa atggaacgag
     4021 cgtggcatcg cattttacga gcggctcggt gcgcagatcg attccgactg gcacgagttt
     4081 caattatcgg aggcggcgat ccgcgacctc gcagcttcgt aactagacgg cgacaggttc
     4141 tttcgattgc gtcgatggcg cgggcgccgg ctcgtcgagc agcttgttga aattgcgcgc
     4201 ctctcggttt ttccaggcgc ctttgtgcca ggcgtagccg gcgatgagcg gcaacgcgat
     4261 tgtcgcctcg gcgtaaacca tctgctctga accctggtcg acttttcccc aggagttcgc
     4321 ttccctgagg gtcgagccgc taagcgcgcc gtcgcgcacg tcggccacgg tgatctgcac
     4381 cgcgtatttg tgcatgccga catcgaaacc gagataatcg gctgcgacca cggtgtcctg
     4441 cgcgaaattt tttgggacgc ctccgccgat catgaacaag ccgctggttt tcgccgcgat
     4501 tttaagccgg gtgagctcga gaaaatcctt cgccgaatcg atgcttacgt gccgatcggg
     4561 attattccac tggtgcgcga ctaagccaaa gccggcgctg caatcgctga aggcggggca
     4621 aaaaattgga agattttgtt cgaatgccgc gagcacaact gaatcgcgcg ttttcgcgtt
     4681 cttcgttagg taatggccca tttctcggat gaactcgcgc gaggaatatg gccgcggctc
     4741 gagcgcatcg gcgatttcgc cgcaggtgtt gtcacaaatc cggagctggt cttcgtcgat
     4801 gtaagtgtcg taaatgcggt cgatggcgag acgacgcagc acgtcatcgt tcatgaagat
     4861 gtcgcccttg tagtgcttga agccgagcgc ttcgaagaaa tcttgatcaa cgatgattgc
     4921 gccggtcgaa acgatcgcgt cgaccatgtt gtgccggaca agatcgacaa tcacttgctt
     4981 cagcccggca gagatgagtg agccggcgag gcagagaatg atggcgcaat ctttctcgcg
     5041 cagcatcgca tcgtaaatat gcgccgcgcg gccgaggtcg cgcgcggaga acgccatctt
     5101 cgacatcacc tcgaccatgt caacgacgtc gaattgcttg atgtcgatgt gctcgatttc
     5161 ttcgacgaga agttcggatt ttttcgggtt catatttcag ggtagcgcaa gcttccagct
     5221 tgcgaataaa acaaatacaa gccagaggct tgcgctactt ttccggatac agccattgca
     5281 tgtaatcacg cacgccgtcc tccagcgggt agcgcggttc gtagccgagt gcgcgccgcg
     5341 cctggctcag gtcggcttcg gtaaagtttt ggtaatgagc atgtgggttc tcgatgtaat
     5401 cgggccggaa tttggtcccg aggcagttat tgagaacatc gataagttcg ttgaatgagc
     5461 gcgcctggcc ggagccgaga ttgtagatcc cgctctcccg cgcttcgagc gcgccaatcg
     5521 tggcggcgac ggcatccttc acataaacga agtcgcgctt ttgttcgcca tgcttgaaga
     5581 tgcgcggccg ctgaccggcc cgcatctgct gcgcgagatg ataaaccatg ctggcagg
//
LOCUS       DASTDG010000073         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_422103, whole genome shotgun sequence.
ACCESSION   DASTDG010000073 DASTDG010000000
VERSION     DASTDG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422103"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="aroF"
                     /locus_tag="VFO30_02330"
     CDS             <1..822
                     /gene="aroF"
                     /locus_tag="VFO30_02330"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9418152.1"
                     /translation="VTKPYKLVTLDLRPEKTVVHVGDATIGGSELAIIAGPCAIESRD
                     QALAVAKAVQKSGARFFRGGIWKPRSSPYAFRGLGPKGWDIMREIREKFGLKVVTEAL
                     NESNIDMIEQYGDVIQIGARNMQNFPLLERAGRSKLPVLLKRGMAATLEEWLLAAEYI
                     MAEGNYNVILCERGVRTFAQHTRNTLDLAAVPAIRSISHLPVIVDPSHGSGNTYMVTP
                     LARAGVAVGADGIMVEVHNDPRVALSDGAQALTPAQYDELIEEIRAIRAATPRLR"
     gene            867..1442
                     /locus_tag="VFO30_02335"
     CDS             867..1442
                     /locus_tag="VFO30_02335"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET9418153.1"
                     /translation="MVLLLDNYDSFTYNLAQYFGELGCELVVKRNDEISLEEVAALRP
                     RHICISPGPCTPREAGISKDVILHFGREIPLLGVCLGHQCIAEAYGGKVIRAPRVVHG
                     KASQIRHNGDALFADLPNPFLAGRYHSLLVERNSLPSCLEITAETDDGEIMALHHREF
                     PVHGVQFHPESVLTQNGRKILERFLSIAAPA"
     gene            1439..>2895
                     /gene="trpE"
                     /locus_tag="VFO30_02340"
     CDS             1439..>2895
                     /gene="trpE"
                     /locus_tag="VFO30_02340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9418154.1"
                     /translation="MTAHISPTLEQFREFAQRGNVVPVFAEFVADGETPISAFQKLSG
                     ATCSFLFESTEKNDVSGRFSFVGSDPRLIVQSFGREIRVIDPAGEQRFAAAEDPLDEL
                     QKLMARYQLAARDDLPRFAGGAVGFLGYEAVKFFEPSVPVAERDELQLPELLFMLTRS
                     VLVFDHRLRTLKVIVNAFVEDGPLEKVYTRAAEAIGEIVRRLAQPTELPFVTNVAAEA
                     VASQSNFGRDEFESAIEQAKEYIRAGDVYQVVLSQRFEASFAGDALGFYRCLRFINPS
                     PYMFCLKLGDFALVGSSPEMHVRLAGDGIDIRPIAGTRPRGATDAEDARNAVELLADD
                     KERAEHVMLVDLARNDIGRVAEFGSVRVSEFMQIERYSHVMHIVSNVVGRLRRECTAF
                     DLIKATFPAGTVSGAPKIRAMQIISELEKTRRGCYAGAIGYIGFDGKLDSCIALRSAV
                     LKNGKAYFQAGAGIVADSDPRREYEETLSKAGAMA"
BASE COUNT          596 a          819 c          868 g          612 t
ORIGIN      
        1 gtcacaaagc cgtacaaatt ggtgacgctc gacttgcgac cggaaaaaac cgttgtccac
       61 gtcggcgatg cgacgattgg cgggagcgaa ctggccatca tcgccgggcc gtgcgcgatc
      121 gagagccgcg atcaagcgct ggctgttgcc aaagccgtgc aaaaaagcgg cgcgcgtttc
      181 tttcgcggcg gaatttggaa gccgcgaagc tcgccttacg cgttccgcgg tcttgggccg
      241 aaagggtggg acatcatgcg cgaaattcgc gagaaatttg gcctcaaggt tgtaaccgaa
      301 gcgctgaatg aatcgaacat cgacatgatc gagcaatatg gcgatgtcat tcagattggc
      361 gcgcgcaaca tgcaaaattt tccgctgctg gagcgggccg gtcgttccaa gctgccggtg
      421 ctattgaagc gcggcatggc ggcgacgttg gaggaatggc tgctcgcagc cgaatacatt
      481 atggccgagg gcaattacaa tgtgatcctt tgcgagcgcg gggtgcgcac gtttgctcag
      541 cacacacgca acacgctcga cctggcggca gtgccggcga ttcggagcat ttcgcacctg
      601 ccggtaatcg tcgatccctc gcacggcagc ggcaacacct acatggtgac cccgctggcg
      661 cgtgcaggcg tcgcggtcgg agccgatggc atcatggtcg aggtgcataa cgacccacgg
      721 gttgcacttt cggatggcgc acaggcactc acgccggcgc aatacgacga actgatcgaa
      781 gagatccggg caattcgcgc ggcgactccg cgtttgcgat gagtaattgt cgatcttgca
      841 tttcttagcc cggagttctt ccagccatgg ttctgctcct cgataactac gactctttta
      901 cctacaacct cgcccaatat tttggcgaac ttgggtgtga gcttgttgta aaacgcaacg
      961 acgagatctc actcgaggaa gtggccgcgc tgcggccgcg acatatctgc atttcacccg
     1021 ggccgtgcac gccgcgcgaa gccggaatta gcaaagacgt tattctgcat tttggccgtg
     1081 agattccgct gctcggtgta tgtctcgggc accaatgcat cgcggaggct tacggcggca
     1141 aggtcatccg tgctccgcgc gtggtgcatg gcaaagcctc ccaaatccga cataacggcg
     1201 acgctttatt tgctgatctg ccgaatccgt ttctcgccgg gcggtatcat tcgctgctcg
     1261 tcgaacgaaa ttcgttgccg agctgtttgg aaattacagc cgagactgac gatggagaaa
     1321 tcatggccct tcaccaccgc gagtttcccg tccacggcgt gcaattccat ccggagtcag
     1381 tgttgacgca gaacggacga aaaatcctcg agcgttttct ctcgatcgct gcacccgcat
     1441 gacggcgcac atcagcccga ctctggagca gttccgcgag tttgcgcagc gcggcaacgt
     1501 ggttccggtg tttgccgaat tcgtcgctga cggtgagacg ccaatttccg cgttccagaa
     1561 actcagcggg gcgacttgca gctttctgtt tgagtcgacg gaaaagaacg atgtgtccgg
     1621 ccggttctcg ttcgtcggca gtgacccgcg tctcatcgta caaagcttcg gccgagagat
     1681 acgtgtgatc gatccagcgg gcgagcagcg tttcgcggct gccgaggacc ctctcgatga
     1741 gttacagaag ctaatggcgc gttatcaatt ggccgcacgc gatgatctgc cccggttcgc
     1801 cggaggcgcg gttggttttc tcggctacga agcggtcaag ttttttgagc cgagtgtacc
     1861 tgtcgccgaa cgtgacgaac tacagttacc cgaactgctc ttcatgctca cgcgcagtgt
     1921 cctcgtattt gatcatcgct tgcgcacttt aaaggtgatc gttaacgcat tcgtcgaaga
     1981 cggcccgctc gagaaggttt atacacgtgc cgccgaagcc attggcgaaa ttgtccgccg
     2041 gctcgcccaa ccaactgagc taccatttgt tacgaacgtc gcggccgagg ccgtggcatc
     2101 gcagagtaac ttcgggcgcg atgagttcga aagcgcgatt gagcaggcaa aggaatacat
     2161 ccgcgccggc gacgtttatc aggttgtctt gtcgcaacga ttcgaggcga gcttcgccgg
     2221 cgacgcgctg ggtttctatc gctgccttcg cttcattaac ccttcgcctt acatgttctg
     2281 cctgaaattg ggtgacttcg cgctcgttgg cagttcgccg gagatgcacg tcaggctcgc
     2341 aggtgatgga atcgacattc gaccgattgc cggcacgcgg ccccgcggcg cgaccgatgc
     2401 cgaagacgcg cgaaacgccg tcgaactgct ggcggacgac aaggaacgcg ccgagcacgt
     2461 gatgcttgtc gatcttgcgc gcaacgacat cgggcgcgtc gcagaattcg gcagtgtgcg
     2521 tgtgagcgaa ttcatgcaga tcgagcgtta tagccatgtc atgcacatcg tctcgaacgt
     2581 cgtcggccgg ctgcgtcggg aatgcacagc attcgatttg ataaaagcga catttccagc
     2641 ggggactgtg tctggtgcgc cgaaaatccg cgccatgcaa atcatcagcg aactggagaa
     2701 aacccggcgc ggctgttacg ccggagcgat cggctatatc ggtttcgacg gtaaactgga
     2761 ttcgtgcatc gcgctccgtt ctgccgtttt gaaaaacgga aaggcatatt tccaagccgg
     2821 cgcgggcatc gtggctgact ccgacccgcg gcgggaatac gaagaaaccc tcagcaaagc
     2881 gggggcaatg gcgaa
//
LOCUS       DASTDG010000074         5894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1034543, whole genome shotgun sequence.
ACCESSION   DASTDG010000074 DASTDG010000000
VERSION     DASTDG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034543"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFO30_02345"
     CDS             <1..462
                     /locus_tag="VFO30_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9418155.1"
                     /translation="EQHPGRALHDAATEAGKDTMFLIRVTHSSGMLEGKYTAETTFPP
                     TDHRSHRPRSWLLNGIKKVEQLRFLENADRTLGQAALQWLLADDRVASTLPNIYNEEQ
                     LVEFAKAPECPSLTRDDLDKIDNLYAKNFGVEEELPKFKGTMGLPRQTAAA"
     gene            complement(479..2710)
                     /gene="priA"
                     /locus_tag="VFO30_02350"
     CDS             complement(479..2710)
                     /gene="priA"
                     /locus_tag="VFO30_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9418156.1"
                     /translation="MMKPSYARVIIDRAIRRELDYAVPETLAERITVGSRVRVPFRDK
                     SALATVVATLEQSDAEGIRPIEAVVGDAPIVSEPLLELAKWMSAYYCCPLETVIRSVL
                     PQVIRKAEVGWKKQLFVGPGGKIDNQELEQLRRRAPRQAELLEAITHLKAPVPAARLL
                     RKLSLDNQTLRALVSRSLAKLSEEAVERDPHADEQFIATSNLRLNEEQTFALKKITEA
                     LASSENARPILLQGVTGSGKTEVYLQAIRATLDRGRSAIVLVPEISLTPQTVERFKGR
                     FAEAHDIVAVLHSHLSEGERHDEWHKIHSGRARIVIGARSAVFAPLKNLGLIIVDEEH
                     ETTYKQEEAPRYHARDVAVVRAKMERCAVVLGSATPSLESYYNATIGKYDLATLTQRV
                     DEQQMPTMRIIDLRQERRKQKSLSILSEKLLQAIADRLEKREQTILFLNRRGFSTSLL
                     CSNCGEARNCPNCSVALTFHRHASRLSCHLCGHTAAVPKKCPACGQDALVYAGFGTEK
                     VESTVSEIFPKAAVRRMDADSMTRKEAYRETLRNFRTGKIDILVGTQMIAKGLHFPNV
                     TLVGIINADLALHLPDFRAGERTFQLLTQVAGRAGRGERTGEVFVQTYTPFSPSIQFA
                     RHHDFAGYFQQELEFRERCDFPPFKHAILITIRSAHEGRAKLSAETLVRRLREAITPE
                     FMLGDAMPAPLEKLQGQFRFHILLRGAAIMRLSRLVRETLDKLPLPEDVAVGVDVDPY
                     QLL"
     gene            2796..3398
                     /locus_tag="VFO30_02355"
     CDS             2796..3398
                     /locus_tag="VFO30_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9418157.1"
                     /translation="MIKFVFKLALCILLALAGCLGYIGLRSGDPLYSVYEWISPARFQ
                     QYDRLIRTAAAQQQLDPMLIKALIWRESRFDTQKHGSAGERGLMQVSERAANEWAREN
                     KIDNFHAEGLFDPQTNLRAGTWYLRRAFDHWQAQPDPLPFALAEYNAGASRVQRWVGG
                     NEAVPLARKEFLRRIDFPATRKYVEQIIARYNFYKRRGRM"
     gene            3470..5557
                     /locus_tag="VFO30_02360"
     CDS             3470..5557
                     /locus_tag="VFO30_02360"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HET9418158.1"
                     /translation="MNNLCHAVANMRRPAGVTCAVAGIMLATFALNAATRAPGPLAPV
                     PSAPAVARHVSHADREAPPNLSQGPATVEIEPPQVSAPTRSSFMTTWQRVPGARGYRL
                     DVATDSSFTQYVEGYRDLDVGNVTGCSVTGLSGGTTYYYRVRAYDSTSTSANSEVMAA
                     STAATAGLVMHAYFDRSITGNPNAAAIEAMINRCVAIFESLFSDRVTINILFRYATTA
                     PDGSRLPSGAIAYSQYVIYPLPWGAYINALRADGKTSNDAKANATLPGSPLSSNIEPA
                     SADGRALGFNTPPAMFGNGDVGDGGPYDGIVTLNSAEPYKFSRPPSPGYFDAQRSTEH
                     EMDEVIGLGSRLGGSSNNLRPQDLFSWSSPGVRNVSPRGTRYFSINGGSTNIVNFNQD
                     SDGDFGDWLSATCPQAHPYVQNAFACTGQFSDIAPTSPEGINLDVIGYNLVSAALPQP
                     TDFNGDKHPDYVLYNTSTRQTVFWFLNNNVLLSGYAGPTLPANWRIADVADFNRDGHP
                     DFALFNPSTLQTVIWYFSGAKLLSGAAGPTLPTGWQLVATADFNADGRPDYVLYNPGT
                     RQTVLWLMNNNVHIGSAFAPTLATGWTIAGVADFNRDGKTDYLLFNAGTGQSRIAYLS
                     GATLLAVHDGPAIGSGYQLVGTSDFNGDAKPDYVLFKPSTGQTQIWYLDNYLFMGTAT
                     GPTLPAGWILAAP"
     gene            5660..>5894
                     /locus_tag="VFO30_02365"
     CDS             5660..>5894
                     /locus_tag="VFO30_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418159.1"
                     /translation="MNKAVEAGGRARRLIALGLCLPVVVITGFAIQAGTRGYGDSAVS
                     DRTVMSGPLVGWHVVRTDSETAIEQMNANQSLAR"
BASE COUNT         1230 a         1771 c         1661 g         1232 t
ORIGIN      
        1 gagcaacatc ccggacgcgc gttgcacgac gccgccaccg aagcaggcaa agacacgatg
       61 ttcctcattc gggtgacgca ttcttccggc atgctggaag gcaaatacac tgccgaaacg
      121 accttcccac cgacggacca tcgcagtcat cgcccgcgca gttggctgct caatggcatt
      181 aaaaaggtcg agcaattgcg ctttcttgaa aacgccgacc gcacattggg ccaagcagcg
      241 ttgcaatggc tgctcgccga tgatcgcgtc gcctccacgc tgccgaacat ctacaacgaa
      301 gaacagctcg tcgagttcgc caaagcgcca gagtgtccgt cgcttacgcg cgacgatttg
      361 gataagatcg acaatctcta cgcgaagaac ttcggcgtag aagaggaact gccgaaattc
      421 aagggcacga tggggctccc ccgacaaacc gcagcggcct agcagttcca attctgagtt
      481 acagcaactg atacgggtcg acgtcgaccc ccaccgccac gtcttcaggc aacggcagtt
      541 tgtccaacgt ctcgcggacg agccggctca aacgcatgat cgctgcgccg cggagcagaa
      601 tgtgaaaacg aaattgtccc tgcaatttct cgagcggcgc gggcattgcg tcaccgagca
      661 tgaattcggg cgtgattgcc tcgcgcaagc gacggacgag cgtttcggcg gagagtttcg
      721 cccggccttc atgcgccgag cggatagtga tcaaaatggc gtgtttgaac ggggggaaat
      781 cgcagcgctc gcgaaactcg agctcctgct ggaaataacc ggcgaaatcg tgatgtcgcg
      841 cgaactgaat ggaagggctg aacggtgtat aagtctggac aaaaacttca ccggtccttt
      901 cgccccgccc ggcgcgaccg gcgacttggg tcagcaattg aaatgtgcgc tcgcccgcgc
      961 ggaaatccgg cagatgcagg gcgagatccg cgttgatgat gccgaccagc gtcacgttcg
     1021 gaaaatgcag gcccttcgcg atcatttgtg tcccgacgag aatgtcgatc ttgccggtgc
     1081 gaaaattccg tagcgtttca cgatacgctt ccttgcgcgt catggaatcg gcgtccatac
     1141 ggcggacggc agccttcggg aaaatctctg aaacggtcga ctcgactttc tcagtgccga
     1201 agccggcgta aaccagcgcg tcctggccgc acgccgggca ttttttcgga acagccgcgg
     1261 tgtgcccgca caaatgacag ctcaaacgcg aagcatgccg atggaacgtg agtgcgacac
     1321 tgcaattcgg acaattgcgt gcttcaccgc aattgctgca caacagcgat gtcgaaaagc
     1381 cgcgccggtt tagaaataga atcgtctgct cgcgtttctc cagccggtcg gcaatagcct
     1441 gcaacaattt ctcggacaat atcgacaagc tcttttgttt gcgccgttcc tggcgaagat
     1501 cgataatccg catggtcggc atctgctgct cgtcgacgcg ttgggtcaaa gtcgcgagat
     1561 cgtatttacc gatggtcgcg ttgtagtaac tctcgagtga cggcgtggcg ctgccgagca
     1621 cgaccgcgca tctctccatc ttcgcccgca cgaccgcaac gtcgcgcgca tgataccgcg
     1681 gcgcttcctc ctgtttgtag gtggtctcgt gctcttcgtc gacgatgatg agcccgagat
     1741 tcttcagggg cgcgaacacc gcactgcgcg cgccgatcac aatgcgggct cgaccggaat
     1801 ggatcttgtg ccattcgtcg tgccgctcgc cttcagacaa atggctgtgt aacaccgcga
     1861 cgatgtcatg cgcttcggca aaacgacctt tgaagcgctc gaccgtttgc ggcgtcagag
     1921 aaatttccgg gaccagcaca atggcgctgc gcccgcggtc gagcgtggca cggatagctt
     1981 gcaaataaac ttccgtcttt ccgctgccgg tcacgccctg gagcaaaatc ggccgggcat
     2041 tttcggacga agcaagtgct tcagttatct tctttagcgc aaacgtttgc tcttcgttta
     2101 gtcgtaggtt gctcgttgcg ataaactgtt cgtcggcgtg cggatcgcgc tcgaccgctt
     2161 cttcgctgag tttggccaag ctgcggctga ccagcgcacg cagggtctga ttgtcgagcg
     2221 acagtttgcg caacaggcgc gcggctggta ccggcgcttt caaatgcgta atggcctcaa
     2281 ggagttcggc ctgtcgcggc gcgcgtctcc gtaactgctc gagctcttga ttgtcgatct
     2341 ttccgccggg accaacaaag agctgtttct tccagccaac ctccgctttg cgaatgacct
     2401 gcggcagcac gctgcggatg actgtttcca gagggcagca gtagtacgcg ctcatccatt
     2461 ttgccagctc gagcagcggt tcactgacga tcggcgcgtc gcccactact gcttcgatcg
     2521 ggcgaatgcc ctcggcatcg ctctgttcga gcgtcgcgac aaccgtcgcc agcgcggatt
     2581 tgtcgcgaaa cggaacgcgc acgcgcgagc cgacagtgat gcgctcagcg agcgtttccg
     2641 gcaccgcgta atcgagttcg cgccgaatcg cgcgatcgat gatcacacgg gcatagctgg
     2701 gcttcatcat ctgcgagacc tcgtaatcgt gctcgtgctc gggcttatca tcgactattt
     2761 atcggcgaag ttttctcgag cacgagcacg attgaatgat caagtttgtt ttcaagctcg
     2821 ccctctgcat tttactcgcg cttgccggtt gcctgggcta catcggcctg cgctccggtg
     2881 atccgctcta cagcgtttac gaatggatca gcccggcgcg ttttcagcaa tacgataggt
     2941 tgatccgcac cgcggcagcg cagcagcagc tcgacccaat gttaattaaa gcgctgattt
     3001 ggcgcgaaag ccggtttgat acacaaaagc acggcagcgc cggcgaacgc ggcctgatgc
     3061 aggtaagcga gcgcgccgcc aatgaatggg cgcgcgagaa taaaatcgac aatttccatg
     3121 cggaagggct tttcgatccg caaaccaatc tacgagccgg cacgtggtat ctccggcgtg
     3181 cattcgatca ctggcaagcg cagccggacc cgttgccatt tgcgctggcc gaatacaatg
     3241 cgggtgccag ccgtgtccaa cgctgggttg gcggaaatga agccgtccct ctagcccgga
     3301 aggaatttct gcgtcggatc gattttcccg ctacgcgcaa atacgtcgag caaatcattg
     3361 ctcgctacaa cttctacaaa cgccgcgggc ggatgtgagc gattctgcag aaaggccaaa
     3421 tcgcaccttg ttattccgtc tcgcgagggt aaagtcggca gttctctcca tgaataatct
     3481 ctgccatgct gtggcgaaca tgcgccgtcc ggccggcgtc acgtgcgctg tcgctggtat
     3541 tatgctcgcc acgtttgccc tgaatgccgc aacgcgcgct ccggggccgc tcgctcctgt
     3601 gccaagcgca cctgcggtgg caagacatgt gagccacgcc gaccgcgaag cgccgcccaa
     3661 tctgagccag ggcccggcaa cggtcgagat cgagccgccg caagtatcgg ccccgacacg
     3721 aagcagcttt atgacgacct ggcagcgtgt gccaggagcg cgcggttatc ggctggatgt
     3781 tgccacagat tcttccttta cgcagtatgt tgaaggatat cgcgatcttg acgtgggaaa
     3841 cgtcaccgga tgcagtgtga ccggcctgag tggcggcacg acctattatt atcgggtccg
     3901 cgcctacgac agcaccagca cgagtgccaa ttccgaggta atggctgcaa gcacggcagc
     3961 caccgccggt cttgtcatgc acgcttactt cgaccgctcg atcaccggca acccgaacgc
     4021 tgccgcgatc gaggcgatga tcaatcgctg cgtcgccatt ttcgagtcgc tgttcagcga
     4081 tcgtgtgacc atcaacattc ttttccgtta tgccaccacg gcaccagacg gttcgcgctt
     4141 gccctctggt gcgatcgcct acagccaata tgtgatctac cccctgccat ggggcgccta
     4201 catcaacgca ttacgtgccg atggtaagac gagcaacgat gcgaaagcaa acgcgaccct
     4261 gcccggctcc cccctttcca gtaacatcga gccggccagt gccgatggcc gagcgctcgg
     4321 tttcaacacg cctccagcaa tgtttggaaa cggcgatgtc ggcgatggcg gcccgtacga
     4381 cgggatcgtc acgcttaatt cagctgagcc gtacaagttc agtcgtccgc ccagtccggg
     4441 gtattttgac gcgcagcgct caaccgagca cgagatggac gaggtgattg ggctgggctc
     4501 ccgattagga ggcagcagta acaatttgcg accgcaagac ttgtttagct ggtcatcgcc
     4561 gggggtcaga aacgtcagcc cgagaggcac acgctacttc tcaataaacg gtggcagcac
     4621 taacatcgtt aacttcaacc aagattccga cggcgacttt ggcgattggc tcagcgcgac
     4681 ctgcccgcag gcgcacccct atgtgcagaa tgcgtttgcg tgcaccggcc agttctccga
     4741 catcgcgccc acctctcccg aaggcattaa tctcgatgtt atcggttaca acttggtgag
     4801 tgcagcctta ccgcaaccaa ctgactttaa cggcgacaaa caccccgact acgtgctcta
     4861 caacacgtcc acgcgccaga cggtgttttg gttccttaac aataatgtct tgctaagcgg
     4921 ctacgctggc ccaactctgc cggcaaactg gcgaatagct gacgtcgccg actttaaccg
     4981 ggacgggcac ccggacttcg ccttgttcaa cccgagcacg ttacagacgg tcatctggta
     5041 tttctctgga gctaagcttc tgtccggcgc tgctggtccc actcttccca ccggttggca
     5101 gttagtcgca acagcggact tcaacgccga cggcagaccc gattacgtgc tctacaatcc
     5161 aggcacgcgc caaaccgtac tctggttgat gaataacaat gtccacatcg gcagcgcctt
     5221 tgcaccgact ctcgcaaccg gctggaccat cgctggtgtc gctgatttta accgcgacgg
     5281 caaaaccgac tacctgctct tcaatgcggg caccggccag tcgaggatcg cctatttatc
     5341 cggagcaaca cttttggcag tgcacgacgg accggcgatt ggcagcggct atcaactcgt
     5401 tggaacgagt gatttcaacg gcgacgccaa acctgattac gtgctgttca agcccagcac
     5461 cggtcaaacg caaatctggt acctggacaa ttatctcttc atggggacag ctaccggccc
     5521 gactctgccg gccggatgga ttttagctgc gccctaatat ggtagtgagc gactcgttca
     5581 ctaagattcc tgactcccaa agcgtcgtta gaataagctt gtaatttcag ggcgacggac
     5641 atagcctgcc aagcctcgaa tgaataaagc agtcgaggcg ggcggccgag cgcgtcggct
     5701 catcgcgctc ggcttgtgtc ttcctgttgt tgttattacg ggcttcgcca tccaagctgg
     5761 cacgcgcggt tacggtgact ctgctgtttc agataggacg gttatgtcgg ggccgctggt
     5821 gggctggcat gtggtgcgca ctgactctga aaccgccatc gaacagatga atgcgaacca
     5881 atcacttgcg cgcg
//
LOCUS       DASTDG010000075         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_3869, whole genome shotgun sequence.
ACCESSION   DASTDG010000075 DASTDG010000000
VERSION     DASTDG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFO30_02370"
     CDS             <1..1002
                     /locus_tag="VFO30_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9418160.1"
                     /translation="QVIFPRKAAAFVRQHAKDFDVIDSLLGTLPFRKPRLRFNGLLVA
                     RSVGLYRLYQKFERLATERWPDQPRGKLLGRMFYRFVKHRLFRASENAVRHCDLLNLP
                     NETEVEALRPGVGSSKPVIVLPYGLTGQQRRALREAAAPAKTRRARRKIAFIGMWSPR
                     KGAKDFGQIIQRVRRSVPDARFIFLGTLVQDRHLLSDLDLPKSDSIQLVVEYQPEELP
                     KLLADVAVGAFPSYAEGFGLAVLEQLAAGIPTVSYDAAGPRAILRDRLPELLVPVGDI
                     DALTAALVRILTCDIADYEGLRQRSFATSERFDWDDIAERTIAAYQAQLDQLSGEGG"
     gene            1002..2096
                     /locus_tag="VFO30_02375"
     CDS             1002..2096
                     /locus_tag="VFO30_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418161.1"
                     /translation="MNTSVLQIVPQLPGSYDGVGDYALILARALRRDYQLDTIFVVAN
                     RADLAEKENFAIVAGLDSLRHFIPQDRHAILHYANYGYQTRGVPFHLRNRMRALRGSG
                     RGRWITTFHELYAFGPPWRSAFWLYPLQVKIARDIIDRSDSCVVSSDVIEQQVHARDP
                     RKPVQLLPVMSNFGEPELNSSLNRSSNHWAICGGTSLILRSLRCFPHAQQATPTHYRA
                     DELEVIGGRASSEVRDAIATLRQTMPRLSCRYHPEVEPARASELLGKCSFAWIDYFAK
                     GKVWPGMIYKSGSFAACCAHGVLPILSHAQPPPPVNGDAFPEWYFASAEFSHFPDSQQ
                     LVRAREKIYAWYHRNASAQRTARAYAEALA"
     gene            2093..>2385
                     /locus_tag="VFO30_02380"
     CDS             2093..>2385
                     /locus_tag="VFO30_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418162.1"
                     /translation="MTRFDDLTLATTVHNNAEMCVAMLRSFEVNIGCAAEVIVLDDGS
                     ASPVTIPPLAWPIRFIRIESALGFCKASDRALREVRTDYALLVDADVLFEPGD"
BASE COUNT          445 a          724 c          691 g          525 t
ORIGIN      
        1 caggtgattt ttccacgcaa ggccgcggca tttgtgcgcc aacacgccaa ggactttgac
       61 gttatcgata gtttgctcgg gacgctgccg tttcgaaaac cgcgattgcg ctttaacggt
      121 ctgctcgtcg cgcgctccgt cgggctctac cggctttacc agaagttcga gcgcttggcc
      181 acggagcgct ggcccgatca accgcgcggc aaattgttag gtcggatgtt ctacaggttt
      241 gtgaagcacc ggctgtttcg cgcctctgag aacgccgtgc gtcattgtga tttgctcaat
      301 ctgcctaacg aaaccgaagt cgaggcgctt cggcccggtg tcggctcgag caaaccggtg
      361 attgttttgc cctacggcct caccggccag cagcggcgcg cgctgcgtga agcggccgcg
      421 ccagccaaaa cgcggcgtgc gcgccgaaag atcgctttta taggcatgtg gagtccacgc
      481 aaaggcgcaa aagactttgg gcagatcatt cagcgcgtcc gccgatcggt tcccgatgcg
      541 cgatttatct ttctgggaac gctggtgcaa gatcgacatc tactgagtga tctcgacctg
      601 ccgaaaagtg attccatcca actcgttgtc gaatatcagc cggaggagct gccgaaactt
      661 ctcgcggacg tcgccgttgg agcgttcccg agttacgcgg agggattcgg cttggccgtg
      721 ttggagcaac ttgccgctgg aattccaacc gtatcgtacg atgcggctgg tcctcgcgcc
      781 attctgcgcg atcgacttcc cgagttgctg gtgccggtgg gcgacatcga tgctctcacc
      841 gcggcactgg ttcggattct cacctgcgac atcgcggatt acgaggggct tcgccaacgg
      901 agtttcgcaa cttcggaacg gttcgattgg gacgatatcg ccgagcgcac aattgcggcg
      961 tatcaagcgc aactcgatca actgagcggg gaaggcggct gatgaacacg agcgttttgc
     1021 aaatcgttcc gcagctcccc ggcagctacg atggtgtcgg cgactacgcg cttattctgg
     1081 cacgcgctct tcgccgcgat taccagctcg atacgatctt tgttgtcgcc aatcgcgctg
     1141 atctcgctga gaaagaaaat ttcgcgatcg tggctggctt ggattcgctt cgacatttca
     1201 ttccgcaaga tcgacatgcc attttgcact atgccaatta cggctatcaa acgcgcggtg
     1261 tgccctttca cctccgcaat cgcatgcgcg ccctccgcgg cagtggccgt ggccgctgga
     1321 tcacaacctt tcacgagctc tatgcgttcg gcccaccatg gcgcagcgca ttttggctat
     1381 acccgctgca ggtcaaaatc gcgcgcgaca tcatcgaccg ctccgacagc tgcgtggtga
     1441 gcagcgacgt aatcgaacaa caggttcacg cgcgtgaccc gcggaagccg gttcagcttc
     1501 tgccggtcat gtcgaacttc ggcgaacccg agttgaactc gagtctcaac cgctcgtcca
     1561 accactgggc gatttgtggt ggcacgtcgc tgatcttgcg ctcattacgc tgctttcctc
     1621 atgcacaaca agcgacaccg acacattatc gggcggatga acttgaggta atcggcggtc
     1681 gtgcaagcag cgaagtgcgt gatgcgatcg ccacactgag gcaaacgatg ccgcgcttgt
     1741 cctgtcgcta ccatccggaa gtggagccag cccgcgcgtc cgagttgtta ggcaagtgct
     1801 cctttgcctg gatcgactac ttcgctaaag gcaaagtctg gccgggcatg atttacaaat
     1861 ccggttcctt cgccgcctgc tgcgcgcacg gcgtgttgcc gatcttgtcc cacgctcaac
     1921 cgcctccgcc ggtgaacggc gacgcttttc cggagtggta tttcgccagc gcagagttca
     1981 gtcattttcc cgactcgcag caactcgtgc gtgcgcgcga gaaaatttac gcctggtatc
     2041 accggaacgc gtccgcgcag cgcaccgcgc gagcatacgc ggaggcattg gcatgacgcg
     2101 cttcgatgac ctgacgctcg cgacgacggt gcacaacaac gccgaaatgt gcgtcgcgat
     2161 gctgcgttcg ttcgaggtga atatcggatg cgccgcggaa gtcatcgtgc tcgatgatgg
     2221 ctcggcctcg ccagtgacga ttcccccact tgcgtggccc atccgtttca ttcgcatcga
     2281 atccgctctc ggtttctgca aagcatccga ccgcgcgttg cgcgaggtac gcaccgacta
     2341 cgcgctgctc gtcgatgccg atgttctctt cgagccgggg gattt
//
LOCUS       DASTDG010000076         5167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_908995, whole genome shotgun sequence.
ACCESSION   DASTDG010000076 DASTDG010000000
VERSION     DASTDG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908995"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFO30_02385"
     CDS             complement(<1..635)
                     /locus_tag="VFO30_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418163.1"
                     /translation="MTRWALPLLAALLACGTGMICGRAALHSVATRDAIGVVFGRGHL
                     LALACGRGIYEADLERAGAERRGAGDNTGTNGQRRSDAAQDRLSEERLLLESTVSAQM
                     AQSLAADEDISAAELASEVDLLRWQFPDERSWRAALGRSHLRLHRLRNDLAAQLRARH
                     WLRRLVNPLHGTAAECRDFYNTHQERFLQPTRCRASHLFLAAPPETPPEIV"
     gene            complement(632..1561)
                     /locus_tag="VFO30_02390"
     CDS             complement(632..1561)
                     /locus_tag="VFO30_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9418164.1"
                     /translation="MRGACEHQLGRVLRVGLAAALSVIGCHSRSANRTPVEIWKQFSG
                     QKALTHVQRQVDLGPRPPGSEAIAKTRQYIDSQLQACGWRVLRQQFVDNTPRGAVQFV
                     NLIARFGPATPSFLLCSHYDTKTFDNVRFVGADDGGSSTGLLLELARVLATQPELASK
                     IELVFFDGEEAYEQFSAHDGLYGSRHFADDLAAQGIVRQFRGGMLFDMVGDRSLDITL
                     PVDSQAAMARDLFASADALGLRKYFTYLSGSGGITDDHTPLNAIGIPTLDVIDFDYAW
                     WHTPADTTDKLSAESLQIVGSVALYYLVNFALK"
     gene            complement(1582..2472)
                     /locus_tag="VFO30_02395"
     CDS             complement(1582..2472)
                     /locus_tag="VFO30_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9418165.1"
                     /translation="MAKTALGKGLGALIGPRAMPTEPQREEIGPRERIYHVSLASVVP
                     SRLQPRKDFAPEALQELVDSIRQHGIIQPLIVRQTGGRYELIAGERRWRAAQELGLSE
                     VPIIIRSANDLEVLELSLIENLQRSDLNPIEEAHGYARLANEFGMRQEEIATKVGRSR
                     AAVANAMRLLDLHPQVQTWVAQGVLSVGHAKVLLGLKNHDEQRAAAETVLRRNLTVRA
                     IERLVARQLGVSRRRKTRSGETVTSTAIADLQERLQRYLGTHVRLHHGDKRGRIEIEY
                     YGDNDLQRIVAAIGLPVNES"
     gene            2537..3445
                     /locus_tag="VFO30_02400"
     CDS             2537..3445
                     /locus_tag="VFO30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET9418166.1"
                     /translation="MAAQILALPVVAHMIVFTLGHSPDPDDAFMFYAMATNKIDLRGY
                     RFEHRLEDIQTLNECALRGELHISAVSIHAYAYITDAYALLPCGASMGDGYGPIVVRK
                     HSTSNMQHPTPKEDLDLRQWLGDCVIAVPGQMTSAFLALQLLLGHFDFIVMPFDQIFD
                     ALKDGRADAGLIIHEGQLTFAESGFEKIVDLGEWWKRETQLPLPLGGNVVRKDIPPDV
                     RHDIVDIIRASIDYGLTHRAEAVGHALAYARDMNKQLADKFIGMYVNDYTRDYGHTGC
                     AAVRRFLGDARDAGYIDKLVEVEFVE"
     gene            complement(3461..3637)
                     /locus_tag="VFO30_02405"
     CDS             complement(3461..3637)
                     /locus_tag="VFO30_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418167.1"
                     /translation="MALLRGLFWLVLFLFFTFCFVVLFEYGTHDFTNGFQKEFARVKA
                     FVVKQTQQVQKPKK"
     gene            3755..4981
                     /locus_tag="VFO30_02410"
     CDS             3755..4981
                     /locus_tag="VFO30_02410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9418168.1"
                     /translation="MKQRTKLPCFSAMRVPLLDLQAQYRTIAEPIHAAIEEVLATQRF
                     ILATKGKEFEEAICRFLAVPHAIGVSSGTDALLATLMALQIGPGDAILTSAYSFFATA
                     ASISRVGATPVFIDIDPATFNLSVSALRSWIDKHSDKARGKLLAPGGETVCAVMPVHL
                     FGLCCAMDEMITIARKHDLLVIEDAAQALGAEYPTDNGPLKAGTMGDAGCFSFYPSKN
                     LGAAGDAGLVACHNEDLARRLRICRHHGMGQEQYQHEFIGGNFRLDEMQAAVLNVKLS
                     FVEQWAGARRAAADFYREEFLRRNLGEQIVLPAEPYRDRGLTNHHVYHQFVIRTRDRD
                     ELRNYLKRREIETAIYYPLGLHEQKCFAYLGYKAGDFLETERTARESLALPIYPEISR
                     EAQSYVVSSIAEFFQS"
     gene            5072..5155
                     /locus_tag="VFO30_02415"
     tRNA            5072..5155
                     /locus_tag="VFO30_02415"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5106..5108,aa:Tyr,seq:gta)
BASE COUNT         1096 a         1541 c         1482 g         1048 t
ORIGIN      
        1 tccacaattt caggcggcgt ttccggcggc gcagccaaaa agaggtgact cgcccgacat
       61 cgtgtgggct ggaggaaacg ctcttggtgc gtgttgtaga aatcccggca ttcagcggcc
      121 gtgccatgaa gcgggtttac cagccgccgc agccagtggc gtgcgcgcaa ctgcgcagct
      181 aaatcattcc gcaaccgatg caggcgcaaa tgacttcgcc caagcgcagc tcgccagctt
      241 cgttcatctg gaaattgcca ccgcagcaga tcaacttcac tcgccagttc cgccgcggag
      301 atatcttcat ccgcggctaa tgactgcgcc atttgcgcag atacggtgct ctccagcaaa
      361 agacgctcct ccgagagtcg atcttgtgcg gcatcggaac ggcgttgtcc attggtgcct
      421 gtgttgtcac cggcaccgcg ccgttcggcg ccggcacgct ccaagtccgc ttcgtagatt
      481 ccgcgcccgc aggccagcgc gagcaaatga ccacgaccaa acacaacgcc aatcgcatcg
      541 cgcgtagcga cggaatgcaa agccgcgcgg ccgcagatca tgcccgtgcc acacgccagc
      601 agagccgcaa gcagaggcaa agcccaccgc gtcatttaag ggcgaagttc accaagtaat
      661 acagcgcgac tgagccaacg atctgcagac tctccgcgct cagcttgtcg gtggtgtcag
      721 cgggcgtgtg ccaccaggcg taatcaaagt cgatcacgtc aagcgtggga atgccgatgg
      781 cgttgagcgg cgtgtgatcg tcggtaatac cgccgctccc gctgagatag gtgaaatatt
      841 tgcgcaggcc gagcgcgtca gccgaagcga acaggtcgcg cgccattgcc gcctgagaat
      901 cgaccggtag tgtgatatcg agagagcggt ctccgaccat atcgaacaac attccccctc
      961 gaaattgccg cacaatcccc tgcgccgcga ggtcatcggc aaaatggcga ctgccgtaga
     1021 gcccatcatg ggcggaaaat tgttcgtagg cttcttcgcc atcgaaaaag accaactcta
     1081 tcttggaagc gagttccggc tgagtcgcaa gaactcgcgc taactcgagc aacaatccgg
     1141 tgcttgaacc gccatcatcc gcgccgacaa aacgcacgtt gtcgaacgtt ttggtgtcat
     1201 agtgagagca gagtagaaat gagggcgttg ccgggccgaa gcgggcgatc aggttgacaa
     1261 actgaacggc gccacgcggg gtgttatcca cgaactgctg tcgcaacact ctccacccac
     1321 aggcttgcag ctgactgtcg atgtactgac gcgtcttcgc aatggcctcg gacccgggcg
     1381 gacgcggacc gagatcgacc tgacgctgaa catgggtcag agccttttga ccggagaatt
     1441 gtttccaaat ttcgaccggc gtcctgttcg ccgagcgcga atggcagccg atcactgata
     1501 gcgcggcagc gaggcccaca cggaggacac gtccgagttg atgctcgcaa gcgcccctca
     1561 tcagctggat ctggggcgaa atcagctttc gtttacaggc aagccgatgg ctgcgacgat
     1621 tcgttgcagg tcattgtcgc cgtagtactc gatctcgatc cggccgcgct tgtcaccgtg
     1681 atgcaatctc acgtgcgtgc caagataacg ttgcaagcgt tcctgtagat ccgcgatggc
     1741 ggtggaagtc accgtttctc ccgaccgtgt tttccggcgt cggctgaccc caagctgccg
     1801 cgcaacgagg cgctcgatcg cgcggacggt taagttgcgg cgcaaaaccg tttcggctgc
     1861 ggcgcgttgc tcatcgtgat ttttcagacc gagcaacact ttggcgtgac caacggagag
     1921 caccccctgc gcgacccaag tttgcacctg cgggtgcaaa tcgagcagcc gcatcgcgtt
     1981 tgcgaccgcc gcacgactgc ggccgacctt agtcgcgatc tcctcctgtc gcatcccaaa
     2041 ctcattcgcc aaacgcgcgt agccgtgcgc ttcctcgatc ggattgagat cgctgcgttg
     2101 caggttctcg atcaacgaca gctcaagtac ttccagatcg ttcgcgctgc ggataataat
     2161 cggcacttcg ctcagcccca gctcctgcgc ggcgcgccag cgacgctcac ctgcgatcaa
     2221 ttcatatcgc ccccctgtct ggcggacgat cagcggctga atgattccat gttgccggat
     2281 cgaatcgaca agctcctgca atgcttcggg agcgaaatct ttgcgcggtt gcagtcgact
     2341 cggcacaacg ctggcgaggc ttacgtgata aattcgctcg cgcgggccga tctcctcacg
     2401 ctgcggctcg gttggcatcg cgcgcggccc aatcaaagcg ccgagacctt taccgagagc
     2461 ggttttcgcc atcggctcag gctatcgaaa caacttcgtg aacgcaaatg acaagagtgc
     2521 cgtgggggat tgcggagtgg ccgctcaaat cttagccttg cccgtagttg ctcacatgat
     2581 tgttttcacg ctcggccatt cacccgatcc ggacgacgcc ttcatgtttt acgcgatggc
     2641 gacgaacaaa atcgatctgc gcggttaccg attcgaacat cggctggaag acattcagac
     2701 attgaacgag tgcgcattgc gcggtgaact gcacatctcc gcggtctcga tccacgcgta
     2761 cgcgtacatc actgacgcgt atgccttgct gccctgcgga gcgagcatgg gcgacggata
     2821 cggtccaatt gttgtcagaa aacattcaac gtccaacatg caacatccaa cgccgaagga
     2881 ggatctcgat cttcgccagt ggctaggcga ttgtgtgatt gcggttcccg ggcagatgac
     2941 gagcgcgttt ctggcgcttc aactccttct cggacacttc gattttatcg tcatgccgtt
     3001 cgaccagatt ttcgacgcgc tgaaagacgg ccgcgccgac gccgggttga tcatccacga
     3061 agggcagctc acgttcgccg agtccggctt tgaaaagatt gtcgatcttg gcgagtggtg
     3121 gaaacgcgaa acgcagctgc cactgccgct tggcggcaac gtcgtgcgga aagacattcc
     3181 gccggacgtg cgccacgaca ttgtcgatat tatccgcgcg agcatcgatt acgggctgac
     3241 gcaccgcgcc gaagcggtcg gacatgcact ggcttacgcg cgcgacatga acaaacagtt
     3301 agccgacaaa ttcatcggca tgtacgtgaa cgattacacg cgcgactacg gtcacaccgg
     3361 gtgcgccgct gttcggcgct ttctcggcga cgcgcgcgac gccggttata tcgacaaact
     3421 tgtcgaggtc gaatttgtcg agtgaatcag tccccggcgg ctatttcttc ggcttttgca
     3481 cctgttgcgt ttgcttcacg acaaaggctt tcacacgcgc gaactccttt tggaaaccat
     3541 tcgtgaagtc gtgcgtgcca tattcgaaga ggacgacgaa gcagaacgtg aagaagagaa
     3601 ataaaacgag ccaaaacagc ccgcggagca acgccatggc tggagggtaa cttagtcgcg
     3661 tggaacggtc aacgctccat cgtcgctccg gctggcgacg taactcagtc gaatttctac
     3721 ggttagctgc acgggcttac gtccaaagtt cgcattgaag cagcgaacga agctgccatg
     3781 cttctcggcc atgcgcgtgc cgctgctgga tctgcaagcg cagtatcgca cgatcgcgga
     3841 gccgattcac gccgctatcg aagaggtact ggcaacgcag cggttcatac tcgcaacgaa
     3901 aggcaaagag ttcgaggagg cgatttgccg gttcctcgcc gtcccgcatg cgattggcgt
     3961 ttcgtctggc acggacgcgc tcttggccac gctcatggcc ctgcagatcg gcccgggcga
     4021 cgcgattctg acgagcgcgt acagcttctt tgccacagcc gcgtctataa gtcgtgtcgg
     4081 cgccacaccc gtcttcatcg acatcgatcc ggccaccttc aatctgtcgg tgtctgcgct
     4141 gcggagttgg atcgacaaac attccgacaa ggcaagggga aagctgctcg cgcccggcgg
     4201 cgaaacggtt tgcgcggtca tgccggttca tctctttgga ctgtgctgtg cgatggatga
     4261 gatgatcacg atcgcgcgca aacatgatct gctcgtaatt gaagatgccg cccaggcgct
     4321 tggcgccgaa tacccgaccg acaatggacc gttaaaagct gggacgatgg gagatgcagg
     4381 ctgtttcagt ttttatccat cgaaaaatct aggcgccgcc ggagacgccg gattggtcgc
     4441 ctgccacaac gaagatttgg cgaggcggct gcgcatttgc cgccatcacg gcatgggcca
     4501 ggagcaatac cagcatgaat tcatcggggg caatttccgc ctcgacgaga tgcaggccgc
     4561 ggtgttgaac gtgaagcttt cttttgtcga acagtgggct ggagcacgcc gtgctgcagc
     4621 agatttttat cgcgaggaat tcctgcgtcg gaatttgggc gaacaaatcg ttcttccggc
     4681 agagccatat cgcgaccgcg gcctaacgaa ccaccacgtt taccaccagt tcgtcatccg
     4741 cacgcgcgac cgcgatgaat tgcgcaacta cctgaaaaga cgcgaaattg agaccgccat
     4801 ttactatccg ctcggcttgc atgagcaaaa gtgcttcgcg tatctcggct acaaagccgg
     4861 tgattttctc gagaccgaac gcacggcacg cgaaagcctc gctctaccga tctacccgga
     4921 aatctcgcgc gaggcgcaga gttacgtggt aagttcgatt gcggaatttt ttcaaagcta
     4981 gaccgctttc tgtgcaggcg agcgcctgct gcttccgggc aaagcacttg ccacgcaagc
     5041 tgcgtcggcc tatatgaacg cccgcacgcg agggtaggtt ccggagtggc caaacggggc
     5101 agactgtaaa tctgctggct ttacgccttc gctggttcga atccagccct gcccactttt
     5161 ctaagac
//
LOCUS       DASTDG010000077         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1660608, whole genome shotgun sequence.
ACCESSION   DASTDG010000077 DASTDG010000000
VERSION     DASTDG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660608"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFO30_02420"
     CDS             complement(<1..371)
                     /locus_tag="VFO30_02420"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET9418169.1"
                     /translation="MTKLRLGVNVDHVATLRQARYATMPESKNAEPDVIVAAAICERA
                     GANGIVAHLRSDRRHMQDRDIQRLRENIMTKLNFEMGNTPEIVDVALRIVPDEVCLVP
                     EKREEVTTEGGLDVVGLRAEL"
     gene            complement(432..1166)
                     /locus_tag="VFO30_02425"
     CDS             complement(432..1166)
                     /locus_tag="VFO30_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET9418170.1"
                     /translation="MKALSGMDERRSSTDVSIVVPVLNEAALIRQFLMHLRERAPGAE
                     IIVVDGGSTDTTAKIAEESGARVMHARRNRAVQMNAGAQIARGDILWFLHVDLTVPAG
                     CLDQIACSLADPKTVGGFFRIRLPRPGFIYRLTDEFAHYAGILLRMRCGDHGFFCRRR
                     DFFALGGFPEVPLMEDVAFFRLLRRRGHVCVSKTRIVANDRRYQVVGPTRLTFAYGLI
                     GLLYIFGASLSFLSRIYDRLCCRRHA"
     gene            1204..>2096
                     /gene="arsS"
                     /locus_tag="VFO30_02430"
     CDS             1204..>2096
                     /gene="arsS"
                     /locus_tag="VFO30_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HET9418171.1"
                     /translation="MNKFAETLARHSRSLRRTRPEILQVNVGKLCNLTCRHCHVNAGP
                     RRREIMTRMTINRVIDWLAKTDIAIVDLTGGAPEMIPGFRYFIERVRALQPRRHIIDR
                     CNLTILLEPGYEDLAAFLARNKVEVIASMPCYSPANVDAQRGEGVFDASIKALQLLNS
                     LGYGGDVNLPLHLVYNPVGPFLPPPQNELEADFKRELFSHFGVVFNKLYTITNLPIGR
                     FAADLRHNGRLDEYMELLMNAFNSATIDGLMCRNTLSVGWRGEVYDCDFNQQLGMQWE
                     NGKPLFLWDIDPDKIDNREIMT"
BASE COUNT          445 a          620 c          596 g          435 t
ORIGIN      
        1 tcgagctcgg cgcgcagccc gaccacgtcg agcccgccct ccgtcgtgac ttcctcgcgc
       61 ttttcgggaa cgaggcagac ctcgtcgggc acgatgcgca atgcaacatc gacaatctcc
      121 ggggtgttcc ccatttcgaa gttgagtttc gtcatgatat tctcgcgcag ccgttggatg
      181 tcgcggtcct gcatgtgccg gcggtcgctt cgcagatggg cgacgatgcc gttagcacct
      241 gcgcgctcgc aaatcgctgc cgcaacgatt acatccggct cagcattctt tgactcgggc
      301 atcgtcgcat aacgcgcctg ccgcagcgtc gccacgtgat cgacgtttac tcccaaacgc
      361 aatttagtca tctggtcgtg aaatggagca agtgtgcacc caaacggcaa gtgtgccggc
      421 gtcctgtgaa gctaagcgtg ccggcgacaa cagagccgat cgtaaatgcg cgagaggaac
      481 gacagtgatg cgccgaaaat atagagcaac ccgattagtc cataagcgaa cgtgaggcga
      541 gtcggaccga caacttgata ccggcgatca ttcgcaacga tacgcgtttt gcttacgcaa
      601 acgtggccgc gccggcgcaa gagacgaaaa aaggcgacgt cttccatcaa cggtacttcg
      661 ggaaacccgc cgagcgcgaa gaagtcgcgt cgacggcaga agaatccgtg gtcgccgcag
      721 cgcatccgca gcaggatccc ggcgtaatgc gcgaattcgt cggtgagccg gtaaataaac
      781 cctggtcgcg gcagccggat ccgaaaaaat ccgccgacgg ttttcggatc cgcaagcgag
      841 catgcgattt ggtcgaggca tccggccggg acggtaagat cgacatgcag aaaccagagg
      901 atgtcgccgc gcgcgatttg cgcgccggca ttcatttgca ctgcgcgatt ccgccgtgcg
      961 tgcattaccc gagcgccaga ttcttctgcg attttggcgg ttgtatccgt gcttccgccg
     1021 tcgaccacga tgatctccgc accgggcgcg cgttcgcgca gatgcatgag aaactgccgg
     1081 attaacgccg cctcattcag caccggaacg acgatggaaa cgtcggtgct ggatcgtcgt
     1141 tcgtccatgc ctgataatgc cttcatcgcc acgaccggtc acctttatgc gcgcagagct
     1201 gcgatgaaca agtttgcaga aactttggcc cgtcactcgc gctcgctgcg tcgcactcgg
     1261 cccgaaattc tccaggtcaa cgtcggtaaa ctttgcaatc tgacctgtcg ccattgccac
     1321 gtgaacgctg gtcctcgccg cagagagata atgacgcgca tgacgatcaa tcgcgtgatt
     1381 gactggctgg cgaagaccga cattgcaatt gtcgatctta ccggcggtgc gccggagatg
     1441 attccgggct ttcgctactt tatcgagcgc gttcgcgcct tgcagccgcg gcgacacatc
     1501 atcgaccgct gcaacctgac tattctgctc gagcccggct acgaagattt ggcggcattt
     1561 ctcgcgcgca acaaggtcga agtcatcgct tcgatgccat gttactcgcc ggcgaatgtc
     1621 gacgcgcagc gcggcgaagg cgtgttcgac gcgagtatca aagcgctgca acttctcaat
     1681 tcgttaggct atggtggcga tgtcaatctt ccgctgcatc tcgtttacaa tccggtcggc
     1741 ccatttttgc cgccgccgca gaacgagttg gaggccgatt tcaaacgcga gctgttctcg
     1801 catttcggcg tcgtcttcaa caagctctac acgattacca atctgccgat cggccgcttc
     1861 gctgccgatc tgcgacacaa cggcaggctc gacgaataca tggaactgct gatgaatgcc
     1921 ttcaactcgg cgaccatcga cgggctgatg tgccgcaaca cacttagcgt gggttggcgt
     1981 ggcgaagttt acgattgcga ttttaatcag cagctcggga tgcagtggga aaacggaaaa
     2041 ccactttttc tctgggacat tgatccggac aagatcgaca atcgcgagat catgac
//
LOCUS       DASTDG010000078         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_766613, whole genome shotgun sequence.
ACCESSION   DASTDG010000078 DASTDG010000000
VERSION     DASTDG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766613"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="lpdA"
                     /locus_tag="VFO30_02435"
     CDS             complement(<1..1403)
                     /gene="lpdA"
                     /locus_tag="VFO30_02435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9418172.1"
                     /translation="MDKFDVVIIGSGPGGYIAAIRAGQLGLKTALVEKDKELGGTCLN
                     VGCIPSKALLTSSDHFVFAQKEAAKHGIIIDNARVDLAKMQERKNKVVKTFNGGVRAL
                     MKTNKVTTFEGLGTISAPGKVSIKLSSGETQEIETKNIIIATGSAAIELPFAKFDGKT
                     IVSSTEALNFTEAPKKLLVIGAGSIGLELGSVWARLGSEVTILEFLPRIAVGFDLELS
                     NLLQRSLTAQGITFHLETKVSAVKLDNGRIIATAKKGNEELKFEADKALVSVGRKPFS
                     EGLGAEKLGVEFDEKKRIKVDKHFKTNVDGIYAIGDVIAGPMLAHKAEDEGIACVERI
                     AGKDGHINYDAIAGIIYTNPELAGVGLTEDQAKEKGIDYRVGRFPFRANSRALCNDEL
                     DGMVKFVADAKTDRILGAHILQHAASELIAEAVSVIEFGGSSEDLSRTTHSHPTLSEA
                     VKEAAMNVEKRALHIVNR"
     gene            complement(1551..2786)
                     /gene="odhB"
                     /locus_tag="VFO30_02440"
     CDS             complement(1551..2786)
                     /gene="odhB"
                     /locus_tag="VFO30_02440"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9418173.1"
                     /translation="MAIEVKIPAVGESISSGVVSVWHKKSGDFVNAGDALFTLETDKV
                     STEITAEKPGVLEALVPEGQEVKIGEVVATIDDSKSAPEKKSEPVEKKAVAAVAERGP
                     ATSSPATEKPLSPAVRRIVDEEHLEPEKISGTGKGGRITKADALAAVQNRGTTQERIE
                     VIEDRSAEERIRGGEQSHDGRFVRKKMSPLRRKIAAQLVMAQHTAAILTTFNECDMSA
                     VQKLRADSQEAFTKKHGVKLGFMSFFIKACVEALKAVPVVNGRIEEEDFIQNNFYDIG
                     VAVGTERGLVVPVVRDADKKSFAALERDIADYATKAREGKLKIEDLQGGTFTISNGGV
                     YGSLLSTPILNPPQSGILGMHKIMPRPVAIDSKVEVRPMMYLALSYDHRAIDGKEAVT
                     FLIKVKECIEDPHKLPLDF"
     gene            complement(2885..5635)
                     /locus_tag="VFO30_02445"
     CDS             complement(2885..5635)
                     /locus_tag="VFO30_02445"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9418174.1"
                     /translation="MRTSIAARANLELIEENYRRWAEDPTLVDSSWAAFFEGFELGNL
                     PQRNGAPAAAAPAAERREGALQTRVDGLVYAYRTLGHTIARVNPLAEARPQNALLTLR
                     ELGFSEKDLDLRIASKFFLDGQEMTLREMMARLERMYADSIGAEFMHIQNTRMRNWVR
                     HRLESRADKYSTPRQVQVAVLRALMEAESFEIFLHSRYVGQKRFSIQGAESLMVILDS
                     ILHKCPAVGVEEICMGMAHRGRLNVLANFLKKSLRVIFTEFSENYVPDLVAGDGDVKY
                     HLGYETVRKLSAGAEIEIRLSANPSHLEAVDPVVEGTARARQRIRGDTEHRRKVLPLL
                     IHGDAAFAGQGIVAETLNMSQLEGYGTGGTVHLVVNNQIGFTTLPEDARSSMYATDIA
                     KMIEVPIFHVNGDDPLACMQVSQLALDFRQEFGRDVVIDMYCYRRYGHNEGDEPAFTQ
                     PKLYAKIDKHPSVTQRYKRELLEAGILSDDDSASLETEFQLRLEVALENVKAIDKEKA
                     DAQAKFRESTAVFQPEYTSSPVRTAISENTLKTIVEALTRVPEEFNVQPKIKRIVLDH
                     RRKVFENGGPYEWHYAELLAFGSLLLDGTPVRLSGQDSARGTFSTRHAILYDAKTGAP
                     YVPLMHLGEKQARICIYNSLLSEAAVLGFDYGYSLDYPTMLCLWEAQFGDFANGAQTI
                     IDQFIVSAESKWQRPSGIVLLLPHGYEGQGPEHSSGRLERFLQLCAEDNIQVCNLTTA
                     AQYFHVLRRQMKRNFIKPLVIMTPKSLLRADFASSRAEEFTSGRFEEILGSPEVGPPD
                     TTKRVILCSGKVYYDLLQHRAQNKIDNAALIRVEQLYPLAEKKLRAMLKPFPQTAKLI
                     WCQEEPQNMGAWNFIEPRLRAMFKREVTYVGRAASASPAVGALALHKRRQAALVGEAF
                     SG"
     gene            5882..>6790
                     /locus_tag="VFO30_02450"
     CDS             5882..>6790
                     /locus_tag="VFO30_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9418175.1"
                     /translation="MRDPIEDFYAQFFGYYRVRPREIRQTVQSLGSGFIVDPAGYIVT
                     NQHVVERAADLKIKITTNDEKTYSAHYITGDDKKDLAFIKIDANGPFPFINLDNISPN
                     LLGETVIVVGNAVGYGSSISRGVLSATKRDITIDQVDYKNLIQTDAAINPGNSGGPVI
                     DISGRLVGISSAKMAFTPQGVPTQGLGFAIPADVVRDSVAEFRKIAQKQPPNKEPGAE
                     ETSRSNAERLFGIQLQDLTEQLADALGYARGRGVLIAAVEPDSPAEAAGLQRGLVLYR
                     IGKHDVSSVKDAERLLSRASSGTSADL"
BASE COUNT         1356 a         1937 c         1932 g         1565 t
ORIGIN      
        1 cgattgacga tatgcagcgc gcgtttttcg acattcatcg ccgcttcttt gactgcttcg
       61 ctcaaagtcg ggtgcgaatg ggtggtacgg cttaggtctt ccgagctgcc accaaattcg
      121 atgacggaaa ccgcttcggc aatcaattcc gatgcggcat gctgcaaaat gtgcgcgcca
      181 agaatccggt cggtcttcgc atcggcgacg aacttgacca ttccatcgag ctcatcgttg
      241 caaagcgcac ggctgttcgc acggaacgga aacctcccga cgcgataatc gatccctttc
      301 tccttcgcct gatcttcggt aaggccaacg ccggccagct caggattggt gtaaatgatt
      361 cccgcgatag cgtcgtaatt gatgtgcccg tctttacccg caatccgttc cacacacgcg
      421 atgccctcgt cttcggcttt gtgcgccaac atcggcccgg caatcacatc gccgatcgcg
      481 taaatgccat ccacgttcgt cttaaaatgc ttgtccactt tgatccgctt cttttcgtcg
      541 aactctacgc cgagcttttc cgcaccgagc ccttcgctaa agggcttgcg tccgaccgaa
      601 acaagcgcct tgtcggcctc aaactttaat tcttcgtttc cctttttcgc cgtcgcgatg
      661 atacgcccat tgtcgagctt caccgcgcta actttggtct cgagatgaaa cgtgatgccc
      721 tgcgcggtca gcgaacgctg gagcaggttc gacaactcca ggtcgaatcc gaccgcgatg
      781 cgcggcaaaa attcgagaat ggttacttcc gagccgagcc gtgcccaaac cgaaccgagc
      841 tcgagaccga tcgagccggc gccgatgacg agaagtttct tcggcgcttc ggtgaagttt
      901 agcgcttcgg tgctgctgac gattgttttg ccatcaaatt tcgcaaacgg caattcgatt
      961 gccgcgctgc cggtcgcaat gatgatgttt tttgtttcga tttcctgcgt ctcgccactt
     1021 gataatttaa tcgacacttt tccgggcgcg ctgattgtgc cgagaccttc gaaggtcgtg
     1081 actttgtttg ttttcatgag cgcgcgcacc ccgccgttga acgtcttgac gaccttattt
     1141 ttgcgctcct gcatcttcgc aagatcgaca cgggcgttgt cgatgataat gccgtgtttg
     1201 gccgcttcct tctgcgcgaa gacaaaatga tcacttgagg tgaggagcgc cttgctggga
     1261 atgcagccga cgttgagaca agtgccgcca agctctttgt ccttttcgac gagtgctgtc
     1321 ttcaacccaa gctgtccagc gcggatcgcc gcaatgtatc cgcccggtcc tgagccgatg
     1381 attacaacgt caaatttgtc catacagcta tgttgtctct gtcattccga ccgaagtgga
     1441 ggaatctctg gctgttttcc tcgcttcgct cgccaatagc aagagctgtc tcgacttcgc
     1501 tgacatgaca aaggctttgc tcggtatgcg caagacacgt agcgcaatga ttaaaaatcc
     1561 agcggcagct tgtgcgggtc ttcgatgcat tccttcactt tgatcaggaa tgtgaccgct
     1621 tctttcccat ctatcgcgcg atggtcataa ctcagagcga ggtacatcat cggccgaact
     1681 tcgactttgc tgtcgattgc caccgggcgc ggcatgattt tgtgcatgcc caggattccg
     1741 ctctggggcg ggttcaaaat cggcgtgctc aagagcgaac cgtaaacgcc gccgtttgag
     1801 atggtgaacg tgccgccctg gagatcttcg atctttagtt taccctcgcg cgcttttgtc
     1861 gcgtaatcgg cgatgtcgcg ctcgagagcg gcaaaggatt ttttatcggc atcacgtacg
     1921 accggcacca ccaacccgcg ctcggtccca acggcgacgc cgatatcgta gaaattgttc
     1981 tgaatgaagt cttcctcttc aatccggccg ttcacgaccg gcacggcttt gagcgcttcg
     2041 acgcaggctt tgatgaagaa cgacatgaac ccgagtttca cgccatgctt tttcgtgaac
     2101 gcttcttgcg aatcggcgcg cagtttctgc acggcgctca tgtcgcattc gttgaacgtg
     2161 gtcaggatcg cggcggtgtg ctgcgccatc accagttggg cggcgatttt ccggcgcaat
     2221 ggcgacattt ttttgcgaac gaaacggccg tcgtgtgatt gttcgccacc gcgaatgcgc
     2281 tcctcggcgg atcgatcttc gatgacttcg atcctttcct gagtcgtgcc gcgattttga
     2341 accgcagcca acgcgtcggc ttttgtgatg cgaccgcctt tgcccgttcc cgagattttt
     2401 tccggctcga gatgttcttc gtctacgatg cgacgaacag cgggcgagag aggtttttcg
     2461 gtagccggcg acgatgtcgc cggaccgcgt tcggcgaccg cggctacagc ttttttctct
     2521 acgggttcgg attttttttc gggcgcagat tttgaatcgt cgatcgtcgc gacgacttcg
     2581 ccgattttta cttcctggcc ttctggcacg agcgcctcca gcacacccgg tttttcggct
     2641 gtgatctcgg tggaaacctt gtcagtctcg agagtgaaga gcgcatcgcc ggcattgaca
     2701 aaatcgccgg attttttgtg ccagacggaa acgacgccgc tggaaatcga ttcgccgacg
     2761 gccggaattt ttacttcgat cgccatggtg gagctgctac acgcaaacaa acgttgtagc
     2821 ggcgcttgta tcaagcgccg aaactaagta agcaggcgct tggcacaagc gcctctacaa
     2881 tgaatcaccc tgaaaacgct tcgccgacga gcgcggcctg gcggcgtttg tgcagcgcca
     2941 gcgcgccgac agcaggactc gcactcgcgg cgcggccgac gtaagtgacc tcgcgtttga
     3001 acatcgcgcg tagccgcggt tcgatgaagt tccacgcgcc catgttctgc ggttcttcct
     3061 gacaccagat gagcttcgca gtttgcggaa agggtttcag catggcgcga agcttctttt
     3121 ctgctaacgg ataaagctgc tcgactcgga tgagcgccgc gttgtcgatc ttgttctggg
     3181 cgcgatgctg caacagatcg taataaactt tgcccgaaca aaggatcacc cgtttggtgg
     3241 tgtccggcgg accgacttcc ggtgagccga ggatttcttc gaatcgtcca ctggtgaatt
     3301 cttccgcgcg tgacgaagcg aagtcagcgc gcaacaggct cttcggcgtc atgatcacaa
     3361 gcggcttgat gaagttgcgt ttcatctgcc ggcgtaagac gtgaaagtac tgcgccgcgg
     3421 tcgtgagatt gcagacctga atgttatcct cggcgcaaag ctggaggaag cgctcgagcc
     3481 gcccactcga atgctccggc ccctggcctt cgtagccgtg cggtaaaagc aaaacgattc
     3541 cgctcgggcg ctgccatttt gattcggctg aaacgatgaa ttggtcgatg atggtttgcg
     3601 cgccgttggc gaaatcgccg aactgcgctt cccacaggca aagcatggtc ggataatcga
     3661 gcgagtaacc gtaatcgaat ccgagcaccg ctgcttctga gagcagactg ttgtagatgc
     3721 agatgcgcgc ctgtttttcg cccaggtgca tcagcggcac atagggcgcg ccggtttttg
     3781 cgtcgtaaag aatcgcgtgt cgcgtgctaa atgtgccgcg cgcgctgtcc tgaccggaca
     3841 aacgcaccgg cgttccatcg agaagaagtg agccgaacgc gagcagttcg gcgtaatgcc
     3901 attcgtacgg gccgccgttc tcgaacacct tgcgacggtg gtcgagaacg atgcgcttga
     3961 ttttcggctg aacgttgaac tcttccggga cgcgcgtcag cgcttcgaca atggttttga
     4021 gcgtgttttc gctgatcgcc gttcggaccg gcgagctggt gtattccggc tgaaacacag
     4081 cggtcgattc gcggaatttt gcctgggcat cggctttctc cttatcaatt gccttcacgt
     4141 tttcgagcgc gacttccagc cgcaactgga actccgtttc gagtgaagcc gagtcgtcgt
     4201 cgctcaagat gccggcttcc agcagctcgc gtttgtagcg ctgcgttacc gacgggtgtt
     4261 tgtcgatctt agcgtagagc ttcggctgcg tgaacgccgg ctcgtcacct tcattgtggc
     4321 cgtagcggcg gtagcaatac atgtcgatga ccacatcgcg cccaaattcc tggcgaaaat
     4381 cgagcgcaag ctggctcacc tgcatgcacg ccagcggatc gtcgccattt acatgaaaga
     4441 tcggcacctc gatcattttg gcgatatcgg tcgcgtacat cgacgaacgc gcgtcttccg
     4501 gcagggtggt gaagccaatc tggttgttca cgaccaggtg cacggtgccg ccggtgccgt
     4561 aaccctcgag ctgcgacatg ttcaacgttt cggcgacgat gccctgcccg gcgaaagcgg
     4621 cgtcgccgtg gataagaagc ggcaaaactt tccgacggtg ttccgtgtca ccacggattc
     4681 gctgccgcgc gcgcgcggtt ccctcgacca cgggatcgac ggcttcgaga tgactcggat
     4741 ttgcggaaag gcgaatctca atttcggcgc cggcggaaag tttgcggacg gtttcgtaac
     4801 cgaggtgata tttgacgtcg ccatcgccag ccaccagatc gggaacgtag ttctcgctga
     4861 actcggtgaa aatgacgcgc agcgattttt tgagaaaatt cgcaagcaca ttgagtcggc
     4921 cgcgatgcgc catgcccatg caaatttctt ccacgccgac ggccggacat ttgtgcagga
     4981 tgctgtcgag aatcaccatc agtgactcgg cgccctggat agagaagcgt ttctgcccga
     5041 cgtagcgcga gtgcaaaaag atctcgaacg attccgcttc catcaacgcg cgaaggacgg
     5101 cgacctgcac ctgccgcggc gtggaatatt tatcggcgcg tgattcgagc cgatgtcgca
     5161 cccagttgcg catgcgcgtg ttctggatgt gcatgaactc ggcgccgatc gaatcggcgt
     5221 acatcctttc aagccgcgcc atcatctcgc gcagcgtcat ctcctgacca tcgagaaaga
     5281 attttgaagc gatgcgaaga tcgagatcct tttcgctgaa accaagctcc cgcaatgtga
     5341 gcagcgcgtt ttgcgggcgc gcttcggcga gcggattcac gcgcgcaatc gtgtgaccga
     5401 gcgtgcggta ggcgtaaacc agcccgtcga cgcgcgtttg cagggcgcct tcgcgccgct
     5461 ccgctgctgg tgcagctgcg gcaggcgcgc cattgcgctg cggcaggttg cccagctcga
     5521 atccttcgaa gaacgccgcc cagcttgaat caaccaacgt cgggtcctcc gcccagcggc
     5581 ggtaattctc ctcgattagc tcgaggtttg ctcgggctgc aattgaggta cgcatggtta
     5641 aattcaatct gcaggcatta gcgctggcag caagttgaca gtgccgatag cgccccgtta
     5701 cttgcagcgc aaaatgcgag cacgattact tgcgatcacc gcgctaatcg caatttcatt
     5761 tgcaggatcg acgtctactt tcgcgcaggc cgatccggcg gcggaaccgg ctgtcctggc
     5821 ggtcgcgagg gtcttacctg cggtcgttaa catcaacact gagcgcgtgg ttcggcgcac
     5881 ggtgcgagat ccaatcgaag atttctacgc gcaattcttc ggttattacc gcgtccgtcc
     5941 acgcgagatc cgccagaccg tgcaaagtct cggctcgggt tttattgtcg atccggcggg
     6001 ttacatcgtt accaatcagc acgtggttga gcgtgcggcc gatttgaaaa tcaaaatcac
     6061 gacgaacgat gagaagacct acagcgccca ttacatcacc ggcgacgaca agaaagacct
     6121 ggcgttcatc aagatcgacg cgaacgggcc ttttccgttc atcaatcttg acaatatttc
     6181 gccgaacttg ttaggcgaaa cagtgatcgt tgtgggcaac gcggtcggct atggcagctc
     6241 catcagtcgc ggcgtcctta gcgcgaccaa gcgcgatatc acaatcgacc aagtcgatta
     6301 caaaaatctc atccaaactg acgcggcgat taacccgggc aacagcgggg gaccggtgat
     6361 tgatatttcc gggcggctcg tcggtattag ctcagcaaaa atggcgttca cgccgcaagg
     6421 ggtgccgacg caggggctcg gattcgccat tccggcggac gtggtgcgtg acagcgtggc
     6481 ggaattcaga aagattgcac agaaacagcc gcccaataag gagccgggag ctgaggaaac
     6541 atcgaggtcg aacgcggagc ggctgttcgg aattcaattg caggatctga cggagcaact
     6601 cgcggatgcg cttggctatg cccgtggccg cggtgtactc atcgcagccg tggaaccgga
     6661 cagcccggcg gaggcagcgg gcctccagcg tggcttggtt ctttatcgca tcggaaaaca
     6721 cgacgtgagc tcagtgaaag acgccgagcg tctgctcagc cgggcgagta gtggaacgag
     6781 tgccgatctt
//
LOCUS       DASTDG010000079         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_419218, whole genome shotgun sequence.
ACCESSION   DASTDG010000079 DASTDG010000000
VERSION     DASTDG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419218"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VFO30_02455"
     CDS             complement(<1..1398)
                     /locus_tag="VFO30_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418176.1"
                     /translation="MALGLAAASVLLVLLLHQPILRTLLRVAAQHFAAGEHLKLEFRV
                     EGGVLTNLVLKNVHITPTGPSAIESVDVDLARADYNLITLLLHGPTEFLRNVEVQSAR
                     VVLNPAAAPLPPRPPKPHERITLPSIFPDRARFIDVSLIVRNKPHDFALEHVDLVLDP
                     RSPGALHIGHLRLPGGQSWTNLSAQTSYANKNLSVRNFVLDERDQLRVVNVDASKIRS
                     KTLTISVEAALGGPFSGSIWLQQTDYSLNTRLRVRGERIAAATLNKYLALPEDYFSGE
                     IEQLNVDLGGVLNKPDSWNGSVSAQVINFRQPQIALDRCLFVVSAEHGRANLEQAELV
                     QGANGFHLRGSAELPREVKQFGRSAMALEISATAVDLERLTANMPQRFTGSAQLNGKI
                     DISGGKVNANFSASGGSIGFTDGTLDKFTGTLTATKSLAAAEANTPWFADLHSTIQFD
                     VSNFRYRDYLFDAANG"
     gene            1551..2309
                     /gene="gntA"
                     /locus_tag="VFO30_02460"
     CDS             1551..2309
                     /gene="gntA"
                     /locus_tag="VFO30_02460"
                     /inference="COORDINATES: protein motif:HMM:NF041366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis heme-dependent
                     pre-guanitoxin N-hydroxylase GntA"
                     /protein_id="HET9418177.1"
                     /translation="MRPNPFASDLAVANSCLHFASNGELVGRDADSFLHEAHREFRRR
                     IGRAEFPCVGAKAALHEQTYGFAAYSKLASVESTAGLCRDLFEFRERARKIDNYATLI
                     AVFARPLNLSETYFEDLLWRQLRQLHAADVASWAANVSSDPADSHFSFSFAGRAFYVV
                     GMHAASSRAARRFPWPTLVFNPHEQFERLRADGKWKRMQQTIRAREMALQGSINPMLN
                     DFGERSEARQYSGRAVSDDWVAPFPTRAKCPLAH"
     gene            2416..2994
                     /locus_tag="VFO30_02465"
     CDS             2416..2994
                     /locus_tag="VFO30_02465"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HET9418178.1"
                     /translation="MTGRLEPQTGTAFEVAKGQSLRVIDLEGEQVADLVAFNAVDANE
                     WLSSGRSIDYANRIYLTTGDILYSNRSRPMFTILQDDVGRHDFLLTPCSRETFAIIYG
                     QKNYHPSCFENLTTQLARFGIAPDSIPTTFNIFMNVEIDTDGKMSILRPLSRAGDAIT
                     LRAEMDLIVGLTACSAEQSNNFKFKPIGYEIC"
     gene            complement(3093..3857)
                     /locus_tag="VFO30_02470"
     CDS             complement(3093..3857)
                     /locus_tag="VFO30_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9418179.1"
                     /translation="MPVVLVEKQSPHVTVVTLNRPKRRNALTIELLNELIAAIKVASD
                     QPYQRVLILRGAGQAFCTGLDLKEAADTTKAHLTAELVAQTLIDLSQTRLITIAAVHG
                     AAVAGGAGIMSACDFVVAAERTKIGYPEVRRGLVAGLVMTFLRRQIGERHMRDLLLGS
                     ELIEAAKALEIGLVNRVVALDDIMKEAQKFADSVLQGAPGALADTKKLIEKLWSSSVK
                     DDVDLALKYHMQARESPEAREGIAAFNEKRSPRWAN"
     gene            complement(3894..5522)
                     /locus_tag="VFO30_02475"
     CDS             complement(3894..5522)
                     /locus_tag="VFO30_02475"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9418180.1"
                     /translation="MHRKSTSSAGSLHDFSQSIAKEEDVLRQGGGAAGLERQRKLGRL
                     PTRERITALLDKDAEFFEIGLWAAYKMYSEWGNVPAAGCIAGIGNVIGIPSMVIANDA
                     SVKAGAFFPATVKKLLRAQRIAFECSLPILYLVDSAGVFLPMQDEIFPDEDDFGRIFR
                     NNSVISAAGIPQYAAIMGNCVAGGAYLPVLCDKILMTEGSGLYLAGPSLVKAAIGQIV
                     DAEELGGAKMHADISGTVDFREKDDPACLRRLRSLVALLPEAGAGKTATNRLAYKPAK
                     DPESVYDLVSLDGQKNYDARDLLACVVDANSMDEYKSEYGKTIVTTYARIAGRAVGIV
                     ANQRLQVRTKKQGIQMGGVIYSDSADKAARFVMDCNQTGLPIIFFQDVTGFMVGRDAE
                     ESGIIRSGAKLVNAVSNSVVPKITVIVGGSFGAGNYAMCGKAYDPRFIVAWPSARYAV
                     MGAAQASDTIFTIVTRTRDRGDKKARPEELEELREKVKQSYEEQTDIRYGAARGWVDA
                     IIQPHDTRDVLIQLLQYVSRPFPAARFHTGVIQV"
BASE COUNT         1166 a         1698 c         1656 g         1214 t
ORIGIN      
        1 cccattggcc gcgtcgaaca gataatcgcg gtaacgaaag ttcgagacgt cgaattgaat
       61 ggttgagtgc aagtccgcga accatggcgt attcgcctcg gccgccgcca agctcttcgt
      121 cgcagtgagc gtgccggtaa acttatcaag tgtgccatct gtgaaaccga tcgatccgcc
      181 cgacgcgctg aaattcgcgt tcacttttcc gccggagatg tcgatcttgc cgttgagttg
      241 cgccgaaccg gtaaagcgct gcggcatatt cgcggttaag cgctccagat cgacggccgt
      301 cgccgaaatc tccagtgcca tcgcgctacg tccgaattgt ttcacttccc gcggtaattc
      361 agctgagccg cgcaggtgaa atccgttcgc gccttgcacg agctccgctt gttccaaatt
      421 cgcgcgaccg tgctcagccg agaccacgaa aagacaccgg tcgagcgcaa tttgcggctg
      481 gcggaagttg atcacctgcg cagaaacact cccgttccac gagtctggtt tgtttaaaac
      541 gccaccaaga tcgacattta attgttcgat ctctccgctg aaataatctt ccggcagagc
      601 gagatacttg ttcaacgttg ccgcggcaat tctttcgccg cgcactcgca gccgcgtgtt
      661 cagtgaatag tccgtctgct gcaaccaaat cgaaccggag aacggcccac cgagtgccgc
      721 ctcgacgctg atggtcaaag ttttcgaacg gattttcgac gcatcaacat ttacaacccg
      781 taactggtcg cgctcatcga ggacgaaatt acgaacgctc aggtttttgt tcgcataaga
      841 ggtctgcgcg gaaagattgg tccaagactg gccaccggga agccgcaagt ggccgatgtg
      901 caacgcgccg gggctacgcg gatcgagcac aagatcgaca tgttcgaggg cgaaatcatg
      961 cggcttgttc cgcacgatca gactcacatc gatgaagcgc gcgcgatcgg ggaagattga
     1021 aggcagagtg attctctcgt gaggtttcgg cgggcgcggt ggcagcggcg cggcggcggg
     1081 attcaggacg actcgggcgg attgaacctc aacgttgcgc aaaaattccg ttgggccgtg
     1141 aagcagcagc gtaatcaagt tgtaatctgc gcgcgcaaga tcgacatcaa ccgattcgat
     1201 cgcgctcggt ccggtcggtg tgatgtgtac attctttaag acaaggttgg tgagaacgcc
     1261 gccttcaacg cgaaactcga gcttgagatg ttcgccggct gcgaaatgct gcgcggcgac
     1321 gcggagcaac gtccgcaaaa tcggctggtg taacaacagc accagcagaa cagaagccgc
     1381 ggccagtccc agcgccacgc cgcgccagaa ccgcctgcgc gcctctgcgc ttgcatgacc
     1441 ttcctcgccc acgaaagcat ctattctatt ttcaccgccg ccaatcgcaa acgcctgcga
     1501 cgatttatgg tggcgactgc cgcaagcttc tcgcgcgaaa cagatgcaca gtgagaccga
     1561 acccattcgc gagcgacctc gccgtcgcga acagctgcct gcattttgcc tcaaatggtg
     1621 agctggtcgg ccgcgatgca gacagctttt tgcacgaggc acatcgtgag tttcggcggc
     1681 gcattgggcg cgcggaattt ccctgcgtcg gcgccaaggc tgctttgcat gagcaaacct
     1741 atggctttgc cgcgtactcg aagctcgcct ccgtggaatc gacagccgga ctttgccgcg
     1801 atctgttcga gttccgggaa cgcgcgcgca agatcgacaa ctacgcgacg ctcatcgccg
     1861 tttttgcacg cccactgaat ctcagcgaaa cctacttcga agacttactt tggcgccagc
     1921 tgcgtcagct tcacgcagct gacgtagcaa gctgggccgc aaacgtaagc tccgacccgg
     1981 ctgactcgca tttctcattc agtttcgccg gacgcgcgtt ttacgtcgtt gggatgcacg
     2041 cggccagctc gcgcgcggcg cggcggtttc cgtggccgac gctcgttttc aatccgcacg
     2101 agcagtttga acggttgcgc gccgatggga aatggaagcg aatgcagcag acgatccgcg
     2161 cacgtgagat ggcgttgcaa ggcagcatca acccgatgct gaatgacttt ggcgagcgct
     2221 cggaagcgcg ccagtattcc ggccgtgcgg tgagcgatga ttgggttgcg ccgtttccaa
     2281 cgcgcgcaaa atgcccgctg gcgcactaat gtctcccgct cttgcgcagc cctgtgtcat
     2341 gtcgagcgaa gtcgagacat ctcttaccgt ccaggtttga attgaacaat gagagattcc
     2401 tcgacttcgc tcgacatgac aggaaggctg gaaccacaaa ccgggacggc gttcgaggtg
     2461 gcgaaaggcc agtcgctgcg cgtgattgat ctcgaaggcg aacaggttgc tgacttggtc
     2521 gcgtttaatg cggttgacgc gaacgagtgg ctctcgtctg gccggagcat cgattacgcg
     2581 aaccggattt atctcaccac cggcgatatt ctctattcga accgcagccg cccgatgttc
     2641 acgattttgc aggacgatgt cgggcgccac gattttttgc tgacgccgtg cagtcgcgag
     2701 acattcgcga tcatctacgg gcagaagaat tatcatccca gctgcttcga gaacctgacc
     2761 acgcagcttg cacgattcgg catcgcaccg gattcaattc cgaccacgtt caatattttc
     2821 atgaatgtgg agatcgatac ggacggcaag atgtcgatct tgcggccgct ttcgagggcc
     2881 ggcgacgcga tcacgttgcg cgcagaaatg gatttgatcg tcggcttgac cgcctgctcg
     2941 gcggaacagt cgaataactt caaatttaaa ccgatcggct acgaaatctg ctagaacgcc
     3001 cggcaggcac gagtgtgtca ctccagccgg agcaactttt tgccaacact ttcagctgcg
     3061 tctgcatcgc tctccttatg tgcgcggcgc gactaattcg cccaacgcgg ggaacgtttt
     3121 tcgttgaacg ctgcgatacc ttcgcgggct tcgggggatt cccgcgcctg catgtgatat
     3181 ttcaaggcaa gatcgacatc gtccttgacc gagctcgacc agagcttctc gatcagcttt
     3241 tttgtgtcgg cgagcgcgcc cggcgcgcct tgcaaaactg agtcggcaaa cttctgggct
     3301 tctttcatga tatcatcaag cgcaacaacg cgattgacga gaccgatctc cagggccttg
     3361 gccgcttcga tcagttcgga accgagaagc aggtcgcgca tgtgtcgctc gccaatttgc
     3421 cgacgcagga aagtcatgac caaccccgcg acgagtccac ggcgcacttc gggataaccg
     3481 attttcgtgc gttcagccgc gacgacaaaa tcgcatgccg acatgattcc ggccccgccc
     3541 gcgacggcgg caccgtgcac agcagcaatc gtgatcaggc gtgtttggct taaatcaatc
     3601 agcgtctgcg cgaccagttc cgccgtgaga tgcgcttttg ttgtatccgc cgcctccttc
     3661 aaatcgagtc cggtgcaaaa ggcctggccg gccccgcgca agatgagcac gcgctggtac
     3721 ggctgatcgg atgcaacctt gattgcggcg attaattcat tgagcagctc aatcgtgagc
     3781 gcgttgcggc gtttcgggcg gttcagcgtt acgaccgtga cgtggggact ttgtttttcg
     3841 acgagaacga caggcatgag gtgaatgtcg aaatgcgaat cgctgatgtc gacctaaact
     3901 tgaatgacac ccgtgtgaaa acgtgccgcc gggaacggac gtgagacgta ttgcagcagc
     3961 tggatcagaa cgtcgcgcgt gtcgtgcggc tgaatgatcg cgtccaccca gccgcgcgcc
     4021 gcgccgtaac ggatgtcggt ttgctcttcg tagctctgtt tcaccttttc gcgcagctct
     4081 tccagctcct cgggtctggc ctttttgtca ccgcggtcgc gcgtccgcgt gacgatcgta
     4141 aatatcgtgt cggaagcttg cgctgcgccc atcacagcgt agcgcgcgct cggccaggcg
     4201 acgatgaaac gcgggtcgta agccttcccg cacatcgcgt aattgcccgc gccgaacgag
     4261 ccgccaacga tgaccgtgat cttcggcacg accgaattgc tgaccgcatt gacgagtttc
     4321 gccccgctgc ggatgatccc gctttcctcg gcgtcgcgcc cgaccatgaa accggtcacg
     4381 tcttggaaaa agataatcgg cagtccagtt tggttgcagt ccatcacaaa ccgcgccgct
     4441 ttgtcggcgc tgtcggagta aatgacgccc cccatctgaa tgccttgctt ttttgtgcgg
     4501 acttgcagcc gttggttggc gacgatgccg acggcgcgac cggcgatgcg cgcgtaggtg
     4561 gtgacgatgg tcttgccgta ctcggatttg tattcgtcca tcgagttcgc gtcgaccacg
     4621 cacgccagca gatcgcgcgc atcgtaattc ttttgcccgt cgaggctgac tagatcgtaa
     4681 accgactcgg ggtccttcgc cggcttgtag gcgaggcgat tcgtcgcggt ttttcctgcg
     4741 ccagcttccg gcagcagcgc gacgagtgag cgcaagcgcc tcaagcaggc cggatcatcc
     4801 ttttcgcgga aatcgactgt gccgctgatg tccgcatgca ttttcgcgcc gcccaattcc
     4861 tcggcatcga cgatctgacc gattgccgct ttgacgagcg acggtcccgc caggtacaaa
     4921 ccgctgcctt ccgtcatcag aattttgtca cacagcaccg gcagatacgc gccgcccgcg
     4981 acgcaattgc ccatgatcgc ggcgtattgc gggatgccgg cggccgaaat gactgagttg
     5041 ttgcgaaaga tccggccgaa atcgtcctcg tcggggaaga tttcgtcctg cattggcaga
     5101 aacacgccgg cggaatcgac gagatataga atgggcaacg aacattcaaa ggcgatgcgc
     5161 tgggcgcgca gcagtttttt cactgtcgcg ggaaagaacg cgcccgcttt gacgctggca
     5221 tcgttcgcaa tcaccatgga tggaatgcca atcacattgc cgatcccagc gatgcaacct
     5281 gccgccggga cgttgcccca ctcggaatac attttgtaag ccgcccacag gccgatctca
     5341 aaaaattccg cgtctttgtc caacagagca gtgatgcgct cccgcgttgg caatcgcccg
     5401 agcttgcgtt gccgctccaa gccggcggct ccgccgcctt gacgcaggac atcttcctct
     5461 tttgcaatcg actgggagaa atcgtgcaat gatccagcgg aggatgtcga tttgcgatgc
     5521 atgccgcgaa gcacgggtaa gctgcgcgtt agcttagttc aagcggtcct gcgctgtaga
     5581 ggcgctcgct gcggcgagcc gcttagaaga tgtcggtctc ggaggtgtaa cccgcgtctc
     5641 gaatcgatcg cagccgcgcc tgactgccgc gctcggattc cggccggtta attcgttcca
     5701 gcgcgagctt gagcacaaac ggaacagcag cgag
//
LOCUS       DASTDG010000080        12204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_755925, whole genome shotgun sequence.
ACCESSION   DASTDG010000080 DASTDG010000000
VERSION     DASTDG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755925"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFO30_02480"
     CDS             complement(<1..639)
                     /locus_tag="VFO30_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9418181.1"
                     /translation="MKKILLVVSAILACALSAEAKLKVVATLPDFGALAREIGGDKID
                     ILVLAKPTEDPHFVDARPSFVVSLRNADVLIDGGAELELGWLPPLLQNARNPKIDIGK
                     PGRVIASQGIRLMNVPTNVTRAAGDVHALGNPHFTVDPIIAKAIAQHIAQAFAAVDAP
                     NAASYEANYKKFEATINAKLQEWGAAMLPFKGQHLAAYHDSWPYFGHRFGLNI"
     gene            complement(642..2210)
                     /locus_tag="VFO30_02485"
     CDS             complement(642..2210)
                     /locus_tag="VFO30_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418182.1"
                     /translation="MIKKSSSALLLIAPLAIAFGQDAGPPPPTEIEALRQQVQALTET
                     VKTLQQQVQDQQARIDRMNEENAPEQPEPSPITAAASASPGAATAPQFPTQDASVVAT
                     TAAPATSTAASPGAFPTTDTSVVTSTTETSTITPAAPSLTAPITIGGGRNYMNISFDG
                     LFALAYSSARDLDRIEVGDHDPQQRGFNARNIELAFDGAVDPYFEGFANIVFKLDNDN
                     ETEVEVEEAFLQTTSLPFNLQLKAGQFFAAFGRINPTHPHTWDFADAPIVHGRLLGPD
                     GLRGVGAQLSWIVPVPWYSQLILAVQNGRGGTGYSFRNPGDDGIFFDRRTTDREVRGL
                     QDFVWIPRWENSVDLSPTQVVLAGVSGAFGSNETGANSRTQIYGADFLYKWKSANAEG
                     GFPFVKWQTEWMYRRFQAGRGIDKSFPVAETFHDWGIYSQVLWGFKKGWVAGIRGDYL
                     DMEDSKFTDDFERQSRSRVSANLTWYPTEFSKIRLQYNHDFLDETFFLAGRDVDSVFL
                     QFEFILGAHGAHKF"
     gene            2765..3532
                     /locus_tag="VFO30_02490"
     CDS             2765..3532
                     /locus_tag="VFO30_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418183.1"
                     /translation="MTGFRLSSLIVLLAALSPTPPVHELRDVDLSGWDCLTKPGGAAR
                     TQDGQERNRQKNRSAMDFTSIKINEFDTSGFLAQIAEYDRQLGKKHRRLLTPPEKEQL
                     VALENQVVSLTGWLVLAYQGIPESTNCRSHDFLDWHLELSPEPADHPAQVGDPTPIIC
                     EITPRTESLLYRNGVRVQKLAAFIRLADNSFTGTGNEPHKIRVTGYLFWDDEHNREDS
                     DVGPFIGWFSKEGYHHPWRSTAWEVHPVMKVEDLGTR"
     gene            3611..3940
                     /locus_tag="VFO30_02495"
     CDS             3611..3940
                     /locus_tag="VFO30_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM14 family protein"
                     /protein_id="HET9418184.1"
                     /translation="MRQRMGAAKIYFLIFGVLTIIGGIIGYVKAGSLPSIIAGAVTGV
                     LLLVAGALLPEHRAIGLATGFIVSLLLALQFVPKFIRTGRVMPAGMMSILSVVGLVVA
                     IVAWLKK"
     gene            3945..5309
                     /locus_tag="VFO30_02500"
     CDS             3945..5309
                     /locus_tag="VFO30_02500"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HET9418185.1"
                     /translation="MARIRLRTWRRLVLLLLSAIFVAIIYRWISLEKLQRAAPPELSP
                     TPPPTPTPTRPPVITGRLETGRLFNGITVHSSVTTAPGADATTERQDPESYVLDLKLQ
                     ARVPAANKAMDELAKVTPELPRLLPGLAAMVGPDTVSPLFGELYNTKVKALRENLARL
                     DQLLSRHNFYDCQTLLQLQHPQSHRKALLLQADMDVDADGSDADRMPIGAGVTTNFKP
                     STSFRWPKKTAAPNPYLPAFEQQLKRAEDELALKTTAPERKRELRNVIAQLRAEIGTL
                     KKFSFLIGATDPYIVIPGAFTHGRDTARTGDYALVIFGGAIYPAVVGDIGPNDKVGEA
                     SFRIAKQINPAATPYNRPVSDLKVTYLIFSGTAEAPFGPPDLDKLQGRCQKFVDEIGG
                     ATVPLYHWENIIPPPPTPTPSPSPAPTSTSSPSPVGLTPPSPSPEISPTFAFPLPSAT
                     LSRP"
     gene            5430..6518
                     /locus_tag="VFO30_02505"
     CDS             5430..6518
                     /locus_tag="VFO30_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET9418186.1"
                     /translation="MKSLYKRWWFWVGIVVIAGFVVVHTCLAIWVRDYVNRKLSEIKG
                     YRAHVAAVTLHLWRGAYQIHNIDIKKTSGEVPVPFFSAPLVDFSVQWHALIFERALVG
                     NIEIHAPKMNLVNGPTKERQQAPVDEPWAEKIKQLFPLKINRFAVHNGEIHYRDFSRQ
                     PKVDLTVDHVQMVAVNLTNSKKLSKNLIADIRIEGRPLREGDARAQISLDPYARKPTF
                     ALNFEMKDMPLVKLNDFAKAYGNFTFESGTLKMAMEASAKNGAYQGYVEPVFDHMGIF
                     NPEHDADNPISMVWQAILGGITEIVRNYSKDRFGTKIPFSGTFENPKPDVLTTIFNAF
                     RNAFVQAFEGKLPNESVKVPKAEPEKKQ"
     gene            complement(6548..8251)
                     /locus_tag="VFO30_02510"
     CDS             complement(6548..8251)
                     /locus_tag="VFO30_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9418187.1"
                     /translation="MEERTFLQHYRICTREDGSPDEVSRVGAAINYKATDTHSGEPVL
                     LQLIPLESLHSTTLEQVEARAAAAQKLDHVNIAKLFATGTEHGYFALASEYLQGENAD
                     AWIVAHGSMTPDAALHVALQVVRAVGAAAFFGLSHRAVQPSNILIVPGQSPDGGWPLV
                     KLLNFGLAGAELHSVGAETRAIAPAIAPQFASPEQRRNGEIDFRSEMYSLGATMCFLL
                     TGAVPLGPNATAAYLSHLRRFPRPLRTPLGHMLRENPDERPQDPVVFEKEIRNCLLQI
                     ERRQTLGRKLGMPLAGVIRRKPREPLSPLAQVWRGVVAVALLVLAAAAVGALLFPNEV
                     RLWHRSGEIGVPVGVPDSSQFAAAQPANTVPPLEPGATQSPPAPAPAPTSVASTIAQQ
                     VQTGTASAINTATPPAPAPVEPSSSTQIAAAGAAQPAAPPAEGPQAQASPSTARNGQA
                     RSENSAPSTTSARAKSSSSTSARRSTSKRARIAQRRNNADEGAGPYRDRSSVRARVVG
                     TTADGRIILRLPSGRLVFVTPNDSDSDLPTPPRPRVFIQRPYMFSPPPPQYSPDYFPY
                     D"
     gene            complement(8328..8401)
                     /locus_tag="VFO30_02515"
     tRNA            complement(8328..8401)
                     /locus_tag="VFO30_02515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8367..8369),aa:Gln,seq:ttg)
     gene            complement(8504..9148)
                     /locus_tag="VFO30_02520"
     CDS             complement(8504..9148)
                     /locus_tag="VFO30_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418188.1"
                     /translation="MNNALQLISATAALIDNAWKGSDRSEKYLAMLRASIERAELAAA
                     ELVKQTGGAGAKALMHPRLAAFAGRSLPSGGGTRQSILLVDDEAMALALVKRILVEAG
                     YNVVCAQSGFECVELFRNSPDKFDLVLLDLTMPMMNGEETFHRLRHMRPDVPVVLCAG
                     FIQQDQLDRLLEEGLAGLLRKPLAPDEIVNHVRSTLESVKFSRAGESTAYSATL"
     gene            9378..10145
                     /gene="sufC"
                     /locus_tag="VFO30_02525"
     CDS             9378..10145
                     /gene="sufC"
                     /locus_tag="VFO30_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9418189.1"
                     /translation="MNALSVDNLHVTIAAQEIVRGLSLTVPRGEVHAIMGPNGSGKST
                     LAKVMAGHPDYEVTGGKITMDGENLLELEPDERARKGLFLAFQYPSEVPGVTIANFLR
                     AAVQARLPEGEELEATEYYAKLYEKMELLGMDRSFTSRSVNEGFSGGEKKRTEILQMA
                     MVEPKYAILDETDSGLDIDALKTVAHGVNSLRGPHIGVLLITHYQRILNYIVPDYVHV
                     MVQGRIVRSGGKELALELEERGYDWAREAAGEMALAR"
     gene            10169..11578
                     /gene="sufB"
                     /locus_tag="VFO30_02530"
     CDS             10169..11578
                     /gene="sufB"
                     /locus_tag="VFO30_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9418190.1"
                     /translation="MTTETKAVDINRGIGDFAYPEVHVRDAGAGLSEKTIHYISDVKE
                     DPDWVREFRLRGLKTFLEKPLPTHWASKDLETIDFDKIRYYLAPGTGAKRSWDEVPED
                     IKRTFERLGIPEQERKFLAGVEAQFDSEAVYSNIKKAVGEQGVIFVGSTEGLKNHPEI
                     FRKWFGKVIPIGDNKFSALNSAVFSGGSFIYVPPGVKVKHPLQAYFRINAENFGQFER
                     TLIIADEGSEVTYMEGCTAPKFNTTTLHSAVVELVALRGAKIQYITVQNWSANVFNLV
                     TKRGLAQEDAEVKWIDCNIGSRLTMKYPSVVLKGRKARGEVLSIALANNGQHQDTGAK
                     MIHAADDTTSNIISKSISIGTGRATYRGLVHVPKHLKHCKNNTECDALLINATSRTDT
                     YPAITVRGTGNAVQHEASVSQVSAEQIFYMQQRGLTEAQAMSLSVNGFVNDLVRQFPM
                     EYSVELKRLIELEMEGSVG"
     gene            11638..>12204
                     /locus_tag="VFO30_02535"
     CDS             11638..>12204
                     /locus_tag="VFO30_02535"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418191.1"
                     /translation="MPLVVHDEIMQTGSILSGPVESRDLPDWFRAQQHETWQEFESLP
                     SPTRKDQLWRFSNVNLLDLSPFKRDGELSEDDRRNVLKYSRGLDEFSARLIFANDQLI
                     QRDVVSEELKRRGVIFQPLERAIVEHADVFRKHFMSQPAVLGSAKFAALHKALVSSGT
                     FLFVPRGVEIELPIEIFHWLRGDNMSLFP"
BASE COUNT         2624 a         3480 c         3628 g         2472 t
ORIGIN      
        1 gatgttgagg ccaaagcggt gcccgaagta cggccacgag tcgtgatacg ccgcgagatg
       61 ctggcctttg aagggcaaca tggccgcacc ccattcctgt agtttcgcgt taatcgtggc
      121 ttcgaatttt ttgtagttgg cctcgtaact tgcggcattt ggcgcatcga cggcggcgaa
      181 tgcttgcgcg atgtgttgcg cgatcgcttt ggcgatgatt ggatcgacgg tgaaatgcgg
      241 gttgccaagc gcgtgcacgt cgcccgcggc acgcgtcaca ttcgtcggca cattcatcaa
      301 tcggatacct tgtgacgcga tcacgcgacc cggtttgccg atgtcgatct tgggattgcg
      361 cgcgttttgc aagagcggcg gcaaccagcc gagttcgagc tcggcgccgc cgtcgatcaa
      421 cacatcggcg ttgcgcaacg aaacgacgaa gctcggccgc gcatcgacga agtgcgggtc
      481 ctcggtcggc ttggcgagaa cgagaatgtc gatcttgtcg ccgccgatct cgcgcgcaag
      541 cgcgccgaag tctggcaatg tcgcgacgac cttgagtttc gcctcggccg acaacgcgca
      601 ggcgagaatt gcggagacga ctaacaggat ctttttcatg actaaaactt atgggcgccg
      661 tgggcgccga ggataaattc gaactgcagg aaaaccgaat ccacatcacg cccggccaga
      721 aaaaacgtct catcgaggaa atcgtgattg tactgcagcc ggatttttga gaactcagtt
      781 ggataccagg tcaggttcgc gctgacgcgg gaacgtgatt gtcgctcgaa atcatccgtg
      841 aatttggaat cttccatatc gaggtaatcg ccgcgaatgc ccgcaaccca acccttttta
      901 aatccccaca gcacctgcga atagattccc caatcgtgga acgtctccgc gactggaaac
      961 gatttgtcga tgccgcgacc agcttggaag cgccgataca tccactcggt ctgccatttc
     1021 acaaatggaa aaccgccctc ggcgtttgcg ctcttccatt tgtagagaaa atcggcgccg
     1081 taaatctgcg tgcgcgagtt agcaccggtc tcgtttgagc caaacgcgcc cgacactccc
     1141 gcgagcacga cctgagttgg gctgagatcg accgaatttt cccagcgcgg aatccagacg
     1201 aaatcctgca aaccgcggac ctcgcgatcg gttgttcgtc gatcgaagaa aattccgtcg
     1261 tcgccgggat tacgaaatga atagcccgtt ccgccgcggc cattttgcac ggctaagatc
     1321 aattgcgaat accacggcac cggcacgatc cacgagagtt gcgcgccgac gccgcgcaat
     1381 ccatccgggc cgagcaaacg tccgtgaacg atgggcgcat cggcgaagtc ccacgtgtgc
     1441 gggtgcgtcg gattgatccg gccgaaggcg gcgaagaatt ggccggcttt caattgcaaa
     1501 ttgaacggca aactcgtcgt ctggaggaat gcttcctcga cttcgacctc ggtttcgttg
     1561 tcgttgtcca atttgaaaac aatgttggcg aagccttcga agtaagggtc gaccgcgcca
     1621 tcaaaggcga gctcgatgtt gcgggcattg aacccgcgtt gctgcgggtc gtggtcgccg
     1681 acttcgatgc ggtccagatc gcgcgcgctg gagtaagcga gtgcgaacaa gccgtcgaag
     1741 gaaatgttca tataatttcg gccgccaccg atggtgatcg gcgcggtcag cgacggcgcc
     1801 gccggggtga ttgtcgatgt ttcagtcgtc gatgttacaa ctgaagtatc ggtagttggg
     1861 aacgctccgg gcgaagcggc ggtagatgtg gccggcgcag ccgtagtcgc tactacggac
     1921 gcgtcttggg ttgggaactg cggagcggtc gctgcgccag gtgaagccga agcagcggca
     1981 gtgatcggcg atggttccgg ttgctccggc gcattctcct cgttcatacg atcgatcctt
     2041 gcctgttgat cttgcacctg ttgttgcaag gtcttcaccg tctcggtgag cgcctgcact
     2101 tgctggcgca gagcttcgat ctcagtgggc ggcggcgggc cggcgtcctg gccgaacgcg
     2161 attgcgagcg gcgcgatcag taacaaagcc gaactggatt tcttgatcat gattcgtttt
     2221 tcaaaaacag agcgagtcct cagcagagtt gcgttcggca cggattgatt tggcggcgca
     2281 gcgaacgcag caacgccgcc atccgcccga ggcgggacga atcaaccggt caggaaattg
     2341 ccgggggcgc gtgctcgaaa atgctggcga gcagaaagag cgatcgaacc cgaactgggt
     2401 tcagatcagc aacccgcaca aatgtgattg ccgccggtgc cggaacaacg ggtggcggag
     2461 cggaatgctc ataggcgccc gaagcaacga acgtcaccgc gcagctgtgt tcgatgcgat
     2521 tcgcgtcagg gtgaacgcgc tcatgcagct gcggcgaaac gcttagtgcc agcgtccaga
     2581 gaaaggctgc ggaaacgaaa cctgcagtaa cagcgtgccc gcgactgaat gaccgcggca
     2641 ctgtgctcat ggcgcggcga gcttccggcg ctcgcgcgaa ttccgcaaga gatttttgca
     2701 agcagtggca gacgcttgac acaagcgcct ctacaaacaa gaaggccgga gtgatttgga
     2761 attcatgacc ggtttccgat taagcagctt aatcgttctg ctcgcggcgt tgtcgccgac
     2821 gccgccggtc catgaattgc gcgatgtcga tcttagcggg tgggattgcc taacgaaacc
     2881 aggtggtgcg gcaagaactc aggacgggca ggagcgcaac cggcagaaga accgctcggc
     2941 gatggatttc accagcatca agattaacga gttcgatacg tccggtttcc tggcgcagat
     3001 cgccgaatac gatcgccagc tcggaaagaa gcaccggcgc cttttgactc cgccggaaaa
     3061 agagcaactc gtcgccctcg aaaaccaggt tgtgtcgctc accggttggc ttgtgttggc
     3121 ctatcaaggc attccggaat cgaccaactg ccggagtcac gattttctcg actggcattt
     3181 ggagttgtcg cccgaaccgg ctgatcatcc cgcgcaagtt ggcgatccaa caccgatcat
     3241 ctgcgaaatc actccgcgca cggagagtct gctttatcgc aatggcgtac gcgtccaaaa
     3301 gctagctgca ttcattcgct tggccgacaa cagctttacc ggtaccggaa acgagccgca
     3361 caaaattcgc gtgaccggtt acctgttttg ggacgacgag cacaacagag aggacagcga
     3421 tgtcggtccg tttatcggat ggtttagcaa agaaggttat caccatccct ggcgctcgac
     3481 cgcgtgggaa gttcatccgg tgatgaaggt ggaggatttg ggaacccgct aatcgcaagg
     3541 tgaggctaat actcaggtca gtgcaggtgc tcgcctcgca actggacagt ctgcgcgcga
     3601 tagcttacga gtgcggcagc gtatgggcgc ggcaaagatt tacttcctga tttttggcgt
     3661 gctgacgatc atcggtggca tcatcggcta cgtgaaggca ggcagtttgc cgtcgatcat
     3721 tgccggcgcc gtcaccggcg tgttgctgct tgtagccggc gcgttgctgc cggaacaccg
     3781 cgcgatcgga ctcgcgaccg ggttcattgt ttcgcttctg ctcgcgttgc aatttgttcc
     3841 gaagttcatt cgcacaggta gagtgatgcc ggccgggatg atgtcgatct tgagtgtggt
     3901 tggtctggtc gtggcgatcg tcgcctggct caagaaataa gcgcgtggcc cggattcgtc
     3961 tgcggacgtg gcgacggctc gtcctgcttt tgttgagtgc gatttttgtc gcgatcattt
     4021 accgctggat ctcgctcgag aaactccagc gtgccgcgcc gccggaactt tcgccgacac
     4081 ccccgccaac gccgaccccg acgcgtccgc cggtaatcac gggacgactc gagactggca
     4141 ggttgttcaa cgggatcacg gttcactcga gcgtgaccac cgctcccggc gcggacgcga
     4201 caaccgaacg ccaggacccg gagagttatg tgctggatct gaaattacag gcgcgtgtgc
     4261 cggcagcgaa caaagcaatg gatgagcttg cgaaagtcac ccccgagtta ccgcggctgc
     4321 tgccgggcct tgccgccatg gtcggacccg acacggtgtc gccactgttt ggcgagcttt
     4381 acaacactaa agtgaaagcg ttgcgcgaaa acctcgcccg gctcgaccag cttctttccc
     4441 ggcacaattt ttacgattgt cagacgttgc tgcaattgca gcatccgcaa agccatcgca
     4501 aagcgctcct cttgcaggcg gacatggacg tggatgcgga tggctccgac gccgaccgga
     4561 tgccgattgg cgccggcgtg acgacaaact tcaaaccctc gaccagtttt cggtggccga
     4621 aaaaaactgc tgcgccgaat ccgtacttgc ctgctttcga gcagcaactg aaacgtgccg
     4681 aagacgaact cgcactgaaa acgacagcgc cggagcggaa acgcgagttg cgcaatgtga
     4741 ttgcgcagtt gcgcgcagag atcgggacac tgaagaagtt tagtttcctg attggcgcaa
     4801 ctgatcccta tattgtcatt ccgggcgcgt tcacccatgg tcgcgacacg gcgcggacgg
     4861 gtgattatgc gctggtcatt tttggcggcg cgatctatcc ggccgtagtc ggtgacatcg
     4921 ggccgaacga caaagtgggc gaagcctctt ttcgcatcgc caaacagatc aatcccgcgg
     4981 ccacaccata caaccggccg gtaagtgatt tgaaggtcac ctatttgatt ttctctggaa
     5041 cggcggaagc gccgtttggc ccgccggacc tggacaaact gcaagggcgg tgccagaaat
     5101 tcgtggacga gatcggcggg gcaacggtgc cgctgtatca ctgggagaac attatcccgc
     5161 cgccgccaac gccaactcct tcgccgtcac cggcgccaac gtcgacgtcc tctccgtccc
     5221 cggtcggatt gacgccgccg tctccgtcac cagagatttc accgacgttt gcattcccgc
     5281 tgccgagcgc gacgctgagc cgaccgtagt cagacggcca gcaggttgaa ttgttgcgtt
     5341 ctaattaatg gaaggcgctg tgctgccagc gcttttcttc gggtccggac gccgcgcgtg
     5401 ctccgcaaag ggacaaagga attctgggca tgaagtcact ctacaaacgc tggtggtttt
     5461 gggtcggaat cgtcgtgatc gccggctttg tggtggtgca cacttgtctg gcgatttggg
     5521 tgcgcgatta cgtcaatcgc aagctctcgg aaattaaagg ctatcgcgcg catgtcgctg
     5581 ccgtgacgct ccatctctgg cgcggtgctt accagatcca caatatcgac atcaagaaaa
     5641 ccagcggcga agtgccggtc ccgtttttct ccgcgccact ggtcgatttt tcagtgcaat
     5701 ggcacgctct cattttcgag cgcgcattgg ttggcaacat cgagatccac gcgcccaaaa
     5761 tgaacctcgt caatgggccg acgaaggaga ggcaacaggc gccggtcgac gaaccatggg
     5821 cggaaaaaat caagcaactc tttccgctca agatcaatcg tttcgctgtg cacaacggcg
     5881 agattcatta ccgcgatttt agccggcaac cgaaagtaga tctgacggtg gaccatgtgc
     5941 agatggttgc ggtcaacctt accaacagca aaaaactttc caagaacctg atcgcggaca
     6001 tccgcatcga aggacgccca ctgcgcgaag gcgacgcgcg cgcacagatt tcactcgacc
     6061 cgtacgcgcg caaaccgacg ttcgcgctga atttcgaaat gaaagacatg ccgctggtga
     6121 aattaaacga tttcgcgaaa gcctacggca atttcacctt tgaatccggc acactgaaga
     6181 tggcgatgga agcgagcgct aagaacggcg cctaccaggg ctacgtcgag ccggtgttcg
     6241 accacatggg aatctttaat ccggaacatg acgccgacaa tccgatcagc atggtctggc
     6301 aggcgatact tggcggaatc accgagatcg tgcgcaatta ctcaaaggat cgcttcggta
     6361 ctaaaatccc attctccggt acatttgaaa atccgaaacc cgacgtgctg accaccatct
     6421 tcaacgcgtt tcgcaacgcg ttcgtgcagg cgttcgaggg gaaactgccg aacgaaagcg
     6481 tgaaggtgcc gaaagccgag ccggaaaaaa agcagtgacg gttgcgcgtc ggcccgatct
     6541 cccagcgcta atcgtaaggg aagtagtcag gtgaatactg cggtggcggc ggcgaaaaca
     6601 tgtacggccg ctgaataaag acccggggcc gcggaggggt cggcagatcg ctgtcactgt
     6661 cattcggggt gacaaagacg agtcggccgc ttggcaggcg caagatgatg cgaccgtccg
     6721 cggttgtgcc gacgacgcgg gcacgaacgg agctgcggtc gcggtacggc cccgcgcctt
     6781 cgtcagcgtt gttgcgccgt tgtgcgattc gagctcgctt cgatgtggat ctccgcgccg
     6841 aagtggacga ggacgatttg gcccgcgctg aggtggttga tggggcagaa ttctccgacc
     6901 gcgcttggcc gtttcgtgcc gtcgaggggc tggcttgagc ctgcggaccc tctgctggcg
     6961 gagccgcagg ctgcgctgct ccagcggccg cgatctgcgt cgagctgctc ggttctacag
     7021 gcgctggcgc aggtggcgta gccgtgttga tagccgatgc cgtcccggtt tgaacctgct
     7081 gcgcgattgt gctggccacg gaggttggcg caggggccgg agccggaggc gactgagttg
     7141 cccccggttc cagcggcgga acggtgttgg ctggttgcgc tgcggcaaac tgcgagctgt
     7201 ccggcacgcc gaccggcaca ccgatttcac cactgcgatg ccacaaccgt acctcgttcg
     7261 ggaacagtaa cgcgccaacc gcggctgcgg ccagcacgag tagcgcaacc gcgaccacgc
     7321 cgcgccacac ttgagctagc ggagagagcg gctcgcgcgg cttccgccga atgacgccag
     7381 cgagaggcat gcccagtttg cgccccagcg tctgccggcg ctcgatttgc aaaaggcagt
     7441 tgcggatttc tttttcgaag acaaccggat cctgtgggcg ctcgtctgga ttttcgcgca
     7501 acatgtgccc gagaggcgta cgtagtggcc gcgggaagcg gcgcaggtgc gagagatagg
     7561 cggccgttgc attcggtccg agcggcacgg cacctgtgag aagaaaacac atcgtcgccc
     7621 cgagcgaata catttcggag cggaaatcga tctccccatt gcgccgctgc tctggactgg
     7681 caaattgcgg cgcgatcgcc ggcgcaattg cgcgtgtttc cgcgccgaca gaatgcaact
     7741 cggcgccggc gagaccgaag ttcaacagtt tcaccaatgg ccagccgccg tcgggagatt
     7801 ggccaggcac gatcagtatg tttgacggtt gaacggctcg atggctcagg ccgaagaatg
     7861 cggccgcgcc aaccgcgcgt acgacctgca gcgccacatg cagcgcagcg tctggcgtca
     7921 tcgatccgtg cgcgacgatc cacgcatctg cgttttcgcc ctgcaaatat tccgaggcga
     7981 gcgcgaaata gccatgctct gtaccagtgg caaagagctt ggcaatgttg acgtgatcca
     8041 gtttttgggc ggcggcagca cgtgcttcga cctgctcaag cgtcgtgctg tgcaaactct
     8101 cgagcggaat taattgcagc agcaccggtt cgcccgagtg cgtgtccgtg gctttgtaat
     8161 taatcgctgc tcccacgcgg ctgacttcat ccggcgaacc atcctcacgt gtacaaatgc
     8221 gataatgctg taaaaacgtt ctctcctcca tactcccagc cccagcggcc gaacacatcg
     8281 gcacagtaca ataactatcg catgtgcggt cgagttgcgg gggtgcttgg ctaccccgct
     8341 tggattcgaa ccaagaataa cgcctccaaa gggcgctgtg ttaccgttac accacggggt
     8401 aaagcggctt tccgattttc aattgccgat tctgataaga tcgacaatca catctgtaga
     8461 tcgcaaatcg caaaccgcaa tttgcaaatc gctgggctaa ggcctagagc gttgcggaat
     8521 aggcggtcga ctcaccggcg cgtgagaact ttacgctctc gagcgttgac cggacgtgat
     8581 tgacgatttc gtcaggcgcg agtggtttgc gcaatagccc ggcaagcccc tcctcgagca
     8641 agcgatcaag ctgatcctgt tgaataaaac cagcgcataa cacgaccggc acgtccggcc
     8701 gcatatgacg cagccggtgg aacgtctcct cgccattcat catcggcatc gtgagatcga
     8761 gcagcacgag atcgaattta tcaggagagt ttcggaacaa ttcaacgcat tcaaagcctg
     8821 attgcgcaca gacgacattg tagccggcct cgaccaggat acgtttgacc agtgccagcg
     8881 ccattgcttc gtcatcaacg agcaggattg attggcgcgt tccaccgccg ctcggtaggc
     8941 tgcggccggc aaacgccgcc aaccgcggat gcataagcgc tttcgcaccg gcgccgcccg
     9001 tctgtttgac caattccgct gcggccagct ctgcgcgttc gatgctggcg cgcagcatcg
     9061 ccagatattt ttccgaacgg tcgctgcctt tccacgcgtt atcgattagg gcggcggtgg
     9121 cggagatgag ctgcagcgcg ttattcacat cgctggttgc tctccacatc gaactggcag
     9181 cagaaatggt tttaccgtgt gaattcatat gcgggcagcg gaaaccgttc ggttcacgga
     9241 ggtggctggg agccgcgctg ttttgcagca ggtcaagtgc cgtttgatcg gccaaaccat
     9301 ggcgcgttta tgcgcatttt tgctctcaat cgggtcgcat tgccgcgcga aaggcgtata
     9361 gtgttggtcc ccacaagatg aacgcccttt ccgtcgacaa tctgcacgtc accattgcag
     9421 cgcaggagat cgtgcgcggt ctcagcctga cggtgccccg aggcgaggtc cacgccatca
     9481 tgggcccgaa cggttccggt aaaagcacgc ttgccaaggt catggcgggc catcctgatt
     9541 acgaggtcac gggcgggaaa atcaccatgg atggcgaaaa tctgcttgag ctcgagccgg
     9601 acgagcgcgc ccgaaaaggt cttttcctgg cttttcaata cccgagcgag gtgcccggcg
     9661 tcaccattgc aaatttcctt cgcgcggccg tgcaggcgcg gttgccagaa ggcgaagagc
     9721 tcgaagcaac cgaatattac gcgaagcttt acgagaagat ggaactgctt ggtatggacc
     9781 gctcctttac ctcgcgctca gtcaacgaag gtttctccgg cggcgaaaag aagcggactg
     9841 agattttgca gatggccatg gtcgagccga agtacgccat tctcgacgag accgacagtg
     9901 gcctcgacat cgacgcgctc aagactgtgg cgcacggagt gaattcgttg cgcggcccgc
     9961 atatcggtgt actgttaatt acacattatc agcgcatcct gaactacatc gttcccgact
    10021 acgtgcacgt gatggtgcag ggacggatcg tgcgcagtgg cgggaaagag ctggctctcg
    10081 aactcgagga acgtggttac gactgggcgc gtgaggcggc tggcgagatg gcgctggcga
    10141 gataagatcg acaactggaa aatcaacaat gacaactgaa accaaagcgg tggacatcaa
    10201 tcgcggcatc ggcgatttcg cgtatccgga agtccacgtt cgcgatgcgg gcgccggttt
    10261 gagcgagaaa accatccatt acatttccga tgtaaaagaa gatccggatt gggtccgcga
    10321 atttcgtctc cgcggtctca aaacgtttct cgaaaagcca ctgcccaccc attgggcgag
    10381 caaggatctg gagacgatcg acttcgataa aatccgttat tacctcgcgc ctggcacggg
    10441 ggcgaagcgc agctgggatg aagtgccgga ggacatcaag cgcacttttg aacggcttgg
    10501 cattccggag caggaacgaa aattcctcgc cggcgtggaa gcgcagttcg acagcgaagc
    10561 cgtttactcc aacatcaaaa aagcagtcgg cgagcagggc gtcattttcg ttggatcgac
    10621 cgaaggtctg aagaaccatc cggaaatttt ccgaaaatgg ttcggcaaag tcattccgat
    10681 tggcgacaac aaattcagcg cgctcaactc ggcggttttc agcggcggct cgttcatcta
    10741 cgtcccgccc ggtgtgaaag tgaaacatcc gctccaggcc tacttccgca tcaacgccga
    10801 aaattttggc caattcgagc gcacgctcat catcgcagac gaaggctcgg aggtgacgta
    10861 catggaaggt tgcaccgcgc cgaaatttaa caccacgacg ttgcatagcg cggtggtcga
    10921 attagtcgcg ctcagaggcg ccaagattca atacatcacc gtgcaaaact ggtcggccaa
    10981 cgttttcaat ctggtgacca agcgcggcct cgctcaggaa gacgcggagg ttaagtggat
    11041 cgactgcaac atcggctcgc ggctcacgat gaaatatccg agcgtcgtgt tgaagggacg
    11101 caaagctcgc ggcgaggtct tgtcgatcgc gctcgcgaat aatggccagc accaggacac
    11161 cggtgcgaaa atgattcacg ctgccgatga cacgacgtcg aacatcattt cgaagtcgat
    11221 ttccatcggc actggtcgcg cgacttatcg cggtctcgtc cacgtgccga agcacctcaa
    11281 gcattgcaag aacaacaccg aatgcgacgc gcttctcatt aatgcgactt cacgcactga
    11341 cacctatccg gcgatcaccg tgcgcggcac gggtaacgcc gtgcagcacg aagccagcgt
    11401 ctcgcaggtc agcgccgagc aaatcttcta catgcaacag cgtggcctga ccgaagcgca
    11461 ggcgatgagc ttgagcgtga acggattcgt caacgacctc gtccgccagt tcccgatgga
    11521 gtattccgta gagctgaaac ggctgatcga gctcgagatg gaaggctccg tcggttaagt
    11581 ccgacggatc tttgtagcgg cggtgtctga ccgccgaaga ttaattgcaa gatcgacatg
    11641 ccgttagtcg ttcatgacga aataatgcaa accggatcga ttctctccgg tccggtcgag
    11701 tcgcgcgatc tgccggactg gttccgcgcg caacagcacg aaacatggca agagttcgaa
    11761 tcgctgccgt caccaactcg caaagatcag ctctggcgct tctctaatgt caatctcctc
    11821 gatctttcgc cattcaaaag ggacggcgaa ttgtccgagg acgatcgccg caatgttctg
    11881 aaatactcgc gcgggttgga tgaattctca gcgcgcttga ttttcgcgaa cgaccagctc
    11941 atccagcgcg acgtcgtttc cgaagagctg aagagacgag gcgtgatctt ccagccgctc
    12001 gagcgcgcca tcgtggagca cgccgatgtc ttccgaaagc atttcatgtc tcagcccgca
    12061 gtgctcgggt ccgccaaatt cgccgcactg cacaaagcgc tcgtttcgtc cggcacattt
    12121 ctgtttgtgc ctcgcggcgt cgaaatcgaa ctcccgattg aaatcttcca ctggttgcgg
    12181 ggcgataaca tgtcgctctt tccg
//
LOCUS       DASTDG010000081         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1313291, whole genome shotgun sequence.
ACCESSION   DASTDG010000081 DASTDG010000000
VERSION     DASTDG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313291"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="selB"
                     /locus_tag="VFO30_02540"
     CDS             complement(<1..1524)
                     /gene="selB"
                     /locus_tag="VFO30_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET9418192.1"
                     /translation="MRAISLTIFVLFVVEMTQKHFVVATAGHVDHGKSSLVKALTGTD
                     PDRLPEEKARKITIDLGFAELNLRGPGGEEIHAAMIDVPGHEDFVRNMIAGVGSIDLA
                     LLVVAADDGWMPQTEEHLQILEYLGVTEAVIALTKIDISNADRTESEIREQLRGTNFA
                     DAPIVRTSFPASANLTALSRQVSKRDLAMDGIAALKAALATALCHLSPQRDIGKPRLF
                     VDRAFTLRGIGTVVTGTLTGGTLRVGDEVIVQPRTLTARIRSIQNHGRDVDLAMPGMR
                     TAINLSDVEINRDVARGDVLTVVGFEASSTLDVQLQRSLRLQHVAPIKSGATAYLHHG
                     TTRIRARIFLHEDRHLHAGENAAAQLRLDRPVLAFLGDRFVIRDASEQHTLAGGMIRN
                     ADADQNNVDLCVVSELSRRGAARTSLLLRNSRFSADEIHAALERLQREEKVFLHGDIA
                     VAIENWHDWRRRAATEIDREHRAHPEKKGMELHDLRANFAADMANVFDALIVDLCRDD
                     "
     gene            complement(1588..2952)
                     /gene="selA"
                     /locus_tag="VFO30_02545"
     CDS             complement(1588..2952)
                     /gene="selA"
                     /locus_tag="VFO30_02545"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9418193.1"
                     /translation="MHHKISPRSRRYLPAVNKVLDALDHVDLPRRVIVAIVRRELATL
                     RESGKIVSVDDVFRQVMRALDRHVRSRLQPLINGTGVILHTNFGRAPLSEGARELIDQ
                     IATNYSNLEFDLAAGQRGSRAEYLETNLAILCGAEAATIVNNCAAALVLIVRHFTRDK
                     PEIIISRGELVQIGGGFRIGEIVEAAGAKVREVGATNKTSLADYSRAISKTTAMILRA
                     HRSNFFMEGFVDSPSTVELAELARKRRVPLIVDLGSGLIERIEQLGSQDATPAEVLRT
                     GADLVCFSGDKLFGGAQAGIILGKNRLITALKRESLFRALRCDKLTLAALQATVDVHL
                     SDSAQTPALALIKIPKDELRARAANIITRLQGLPAQITVGRGAVKSGGGTSPRAVIPS
                     ISIDIRPENCSPNEFAARLRALTPPVIGYIANRALKLDLRTIFPPQDDIVVDAIRSAC
                     TTAV"
     gene            complement(2937..>3915)
                     /gene="selD"
                     /locus_tag="VFO30_02550"
     CDS             complement(2937..>3915)
                     /gene="selD"
                     /locus_tag="VFO30_02550"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9418194.1"
                     /translation="TQVLRQLPFSRDRNVLVGSAAADDAGVFRLDKKRALVQTVDFFT
                     PIVDDAFQYGEIAAANALSDVFAMGGRPLTGLNIVGMPDDKIDSKTVATILRGGLSKA
                     REANCAVVGGHTVRAPEPLYGMAVTGVVDPRKMLTNANARPGDVLVLTKPLGTGIVTT
                     AIKRNLTSSALARKAIASMSQLNSVGTEISRRGLAHAATDITGFGLLGHLASMCRASK
                     VRAEISVNDVPAISNEVFDLIARDCVPGGTRDNLNAADAVVEWDGTSHAQRVLLADAQ
                     TSGGLLLSVPEINLRKLVKLLQTVRTPCAVVIGRVVRPRRGGSLICITK"
BASE COUNT          774 a         1094 c         1248 g          799 t
ORIGIN      
        1 atcgtcccgg caaagatcga caatcagcgc gtcgaagaca ttcgccatgt ccgccgcgaa
       61 gttcgcccgc aaatcgtgca gctccattcc cttcttttcc ggatgcgcgc gatgttcccg
      121 atcgatctcg gtcgcggcgc gtcgacgcca atcgtgccag ttctcgatcg cgaccgcgat
      181 atcgccatgc agaaagactt tttcctcgcg ctgcaaccgt tccaacgcgg cgtggatttc
      241 gtcggcgctg aagcgagagt tgcgcaacaa cagcgaagtg cgcgccgcgc cgcgtcgcga
      301 aagttccgat acgacgcaaa gatcaacatt attttgatcc gcatccgcat ttcgaatcat
      361 gccgccggcg agggtatgtt gctctgatgc gtcgcgaatg acgaagcgat cgccgagaaa
      421 tgcgagcacg ggacggtcga gtcgcagttg agccgccgca ttttcgccgg cgtgaagatg
      481 tcgatcttcg tgcaaaaata ttctggcgcg aatgcgggtg gtaccgtgat gcaaatacgc
      541 cgtcgcgccg gacttgattg gcgcgacatg ttgcaaacgc agcgatcgtt gcagctgaac
      601 atccagcgtg ctgctcgctt cgaaaccaac gacagtgaga acatcgccac gcgcgacgtc
      661 gcggtttatt tccacatcgc tgagattgat cgcggtgcgc attccgggca tggcaagatc
      721 gacatcgcga ccgtggtttt gaatggagcg aatgcgcgca gtcagagtgc gaggctgcac
      781 gatgacttcg tcgccaaccc gcaatgttcc gccggtcagc gtgccggtca cgacggtgcc
      841 gatcccgcgc aaggtgaagg cgcgatcgac aaacaggcgc ggtttgccga tgtcacgctg
      901 cggactgaga tggcagagcg ccgttgccaa cgctgccttc aaagctgcaa taccgtccat
      961 cgcgaggtcg cgttttgaaa cttgacggct taaagccgtc aagttagcgg acgcggggaa
     1021 agaggtgcga acgatcggtg cgtcagcgaa gtttgttccg cgcaattgtt cgcggatctc
     1081 gctttcggtt cggtccgcgt tgctgatgtc gatcttcgtg agcgcgatca cagcttccgt
     1141 gacgcccagg tattccaaaa tctggaggtg ctcttcggtt tgcggcatcc agccgtcatc
     1201 ggcggcgaca acaagcaggg cgagatcgat cgagccgacc ccggcaatca tgttgcgcac
     1261 gaaatcttcg tgccccggaa cgtcgatcat cgcggcgtgg atctcttcgc cgccagggcc
     1321 gcgcagattt agttcggcga aaccgagatc gatcgtgatt tttctcgcct tttcctcagg
     1381 caaacgatcc ggatcggttc cggtgagtgc tttgaccaac gagctcttgc cgtgatcgac
     1441 atgaccggcg gtcgctacga cgaaatgctt ctgcgtcatt tcaaccacga agagcacgaa
     1501 gatcgtaagg gaaatcgccc tcactggcgc ttcgcgtccg tctctcccgg cgggaaagag
     1561 gaataagccg tgacaaacca ataattgtca tacggcagtg gtgcacgctg aacggatggc
     1621 atcgacaaca atatcgtcct ggggcggaaa gatggttcgc aaatcgagct tcaatgcgcg
     1681 gttcgcgatg taaccgatga cgggcggcgt gagcgcgcgc aaccgcgcgg cgaattcgtt
     1741 aggcgagcag ttctccgggc ggatatcgat gctgatggaa ggaatgacgg cacgcggcga
     1801 cgtgccgccg ccggatttca cagcgccgcg accgacggtg atttgcgccg ggagtccctg
     1861 caaacgagtg atgatgtttg cggcgcgggc acgcagctcg tctttgggaa ttttgatcaa
     1921 agcgagcgcg ggagtttgag cggagtcgct gaggtgaaca tcgacagtcg cttgaagcgc
     1981 cgcgagcgtg agcttgtcgc agcgcagcgc gcggaagagc gattcgcgtt tgagggcggt
     2041 gatgagtcgg ttcttgccga gaatgatccc ggcctgcgcc ccgccgaaaa gtttgtctcc
     2101 gctaaagcaa acgagatcgg cgccggtgcg tagcacttcc gccggcgtgg cgtcctggct
     2161 gccaagctgc tcgattcgtt cgatcagccc gctgccaaga tcgacaatca gcgggacgcg
     2221 gcgtttgcgt gcgagttccg cgagctccac cgtggaaggc gaatcgacaa agccttccat
     2281 gaagaaattg ctgcggtgtg cgcgcaggat catggccgtg gttttggaga tggcgcggct
     2341 gtaatcggcg agactggttt tgttggtggc gccgacctcg cgcacttttg cgccagcggc
     2401 ttcgacgatt tcgccaatgc ggaagccgcc gccgatctgg acgagctcgc cgcgtgagat
     2461 gattatttct ggtttgtccc tcgtaaaatg gcgcacgatt aggacgagcg cggcggcgca
     2521 gttgttgacg atggtggcgg cttcggcgcc gcagagaatc gcgagattgg tctcgaggta
     2581 ttcggcgcga ctgccgcgct gaccagctgc gagatcgaac tccaggttgc tatagtttgt
     2641 cgcgatttgg tcgatcagct cgcgtgcacc ttcactcagc ggcgcgcgac cgaagttggt
     2701 gtgcagaatg accccggtgc cgttgatgag cggctgcaaa cgcgagcgaa catgtcgatc
     2761 taacgcgcgc atcacctgac ggaacacgtc gtcaacgctg acgattttgc cggactcgcg
     2821 cagggtggcg agctcgcgac gcacgatcgc gacgatgacg cgccgcggaa gatcgacgtg
     2881 atcgagcgcg tcgaggactt tgttgacggc gggcagataa cggcgcgacc gcgggcttat
     2941 tttgtgatgc atatcaacga gccgccgcgt cgcggccgaa cgactctgcc gatgacaaca
     3001 gcgcagggtg tgcgcacagt ttgcagcaac ttcacgagtt ttcgcagatt gatttcgggg
     3061 acgctgagca gcagaccacc gctagtttgc gcgtcggcga gcaagacgcg ttgggcgtgc
     3121 gacgtaccat cccattctac cactgcgtcc gcggcgttca ggttgtcgcg cgtgccgccg
     3181 ggaacgcagt cacgcgcgat caggtcgaac acttcgttgc tgatcgcggg aacgtcgttt
     3241 acagaaattt cggcgcggac tttactggcg cggcacatgg aagcgaggtg accgagcagg
     3301 ccgaagccgg tgatgtcggt ggcggcgtgt gctaacccgc gtcgcgagat ttcggtgccg
     3361 acactgttga gttgcgacat ggaagcgatt gctttgcgtg cgagcgcact ggaggtgaga
     3421 ttgcgtttga ttgcagtcgt gacgattccg gtgccgagcg gtttggttaa gacgaggacg
     3481 tcgcctggac gggcgtttgc gttcgtcagc atcttgcgtg gatcaaccac tccggtgacg
     3541 gccatcccat agagtggctc cggcgcgcgg acggtgtgac caccaaccac cgcgcagttt
     3601 gcttcgcgcg cttttgacaa gccgccacgc aaaatcgtcg cgactgtttt ggaatcgatt
     3661 ttgtcatccg gcattccgac aatattcaat ccggtaagcg ggcgcccacc cattgcgaaa
     3721 acgtcggaga gcgcgttggc ggctgcgatt tcaccgtatt gaaatgcatc atcaacaatc
     3781 ggggtgaaaa aatcgacggt ttgcacgagc gcgcgttttt tatcgagacg gaatacgccg
     3841 gcgtcatcgg cggcggcgga gccgacgaga acgttgcgat cgcggctgaa cggtagctga
     3901 cgcaaaactt gcgtc
//
LOCUS       DASTDG010000082         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_604286, whole genome shotgun sequence.
ACCESSION   DASTDG010000082 DASTDG010000000
VERSION     DASTDG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604286"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFO30_02555"
     CDS             complement(<1..897)
                     /locus_tag="VFO30_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9418195.1"
                     /translation="MPAHAPAEQTASGEFARQEDAFREWVSNDGSTVYPAAAGRYHLY
                     VSLACPWASRTIILRKLKGLENAIGMTIVDPIRDEKGWAFRQSSGKFPPNDSFTSTDP
                     VNGFHFLSEAYTATNPNFEERVTVPVLWDKETSKIVNNCEDDICRMFNNVFDAFARNK
                     DLDFFPQEIEAEHNQLCDFLYDNVNNGVYKAGFATRQRPYEISCRKLFEALDQLEQRL
                     TKSRYLFGNRIVEADWRLFCTLVRFDVVYHGHFKCNIRRVLDYPSLQGYLMDLYQQPR
                     IAETVNFEHIKRHYYMTHEEINP"
     gene            complement(928..1911)
                     /locus_tag="VFO30_02560"
     CDS             complement(928..1911)
                     /locus_tag="VFO30_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9418196.1"
                     /translation="MVLDKPGKPLALRDVAKPKPVTGQVLIRVSTCAVCRTDLHIVDG
                     ELPNPKLPLILGHQIVGRIEEIADHIAGFKTGDRVGIPWLGWTDGDCVYCRSSRENLC
                     DRAKFTGYTIDGGYAEFTVADARFCFHLPNEYHDVDVAPLLCAGMLGYRSYRKTGDAK
                     RLGIYGFGNAAHLITQIAVYQGREVFAFTRPGDRAGQKSARSLGAVWAGGSDELPPEK
                     LDAAIIFAPVGTLVPVALRALAKGGIVVCGGIHMSDIPSFPYVDLWNERTICSVANLT
                     RRDGEEFLEIALRVPVKTKTELFPLEAANSALDNFRTGKLDGTAVLLIDSF"
     gene            complement(2098..3732)
                     /locus_tag="VFO30_02565"
     CDS             complement(2098..3732)
                     /locus_tag="VFO30_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418197.1"
                     /translation="MTEDDLKQARILIVDDEVSNICLLQSVLNRTGFLNHTSISDSRQ
                     ALQRILDWQPDLLLLDLNMPHLSGFDVMQWMRVALPPDNYLPVLVLTADQNPETKRRS
                     LAAGATDFLPKPFDMTEALMRIRNLLNTRLLHLELQKHNALLEQRVSARTADLSRALA
                     ELKQTQQQIVQQERLRAFGEMAGGIVHDFNNALMSIIGYSDLLIHDPATLDDRETTLD
                     YLKTLNTAGRDAAHVVSRLRDFYRGREIEDVFAPLDLNEVIEESASLTQPKWKTQALA
                     SGRTISVEFDLEKLPLISGNPSELREVMTNLIFNAVDAMPEGGTITLRSRRDEDDVLF
                     EIADTGSGMSEAVRNRCLEPFFSTKGDKGTGLGLSMVFGIIKRHNGTLDIESTLGRGT
                     TFRIRLPAAETSDGTIEDTAKLDRSIRVLVVDDDTVPRDVVAKYLVADGHHVSTATSG
                     GEAMTKLTEGNFDLLLTDQTMAGMTGLQLAAAVKQLGGGQPVILMTGSSETASIVDQH
                     ADVSVVLRKPIPQQRLRQALHQILNTGAAEASRRPG"
     gene            complement(3792..4187)
                     /locus_tag="VFO30_02570"
     CDS             complement(3792..4187)
                     /locus_tag="VFO30_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418198.1"
                     /translation="MQKHKILIVDDDPKLSRLVKTILEKTNAYQVQEENRSSAALAAA
                     KAFTPDLVLLDVDMPGKDGGDVARELQADSVLRQTPILFLTSLISHSEAGRGAVERGG
                     MLFLAKPVDPALLIETIGRILKSEPAAAK"
     gene            4372..>4758
                     /locus_tag="VFO30_02575"
     CDS             4372..>4758
                     /locus_tag="VFO30_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418199.1"
                     /translation="MLSSDTSNTRKPGTLAATATALIAGLVLIGWTFDVDLLKRVVPG
                     LVAMNPMTAVAFLLGAISLFLFSDEKSDGRASFHPPLARGCALLIALIGVAKLVAIAR
                     GIDLGVDRWLFSSKLTLGFQVPNVMAP"
BASE COUNT         1007 a         1260 c         1433 g         1058 t
ORIGIN      
        1 tggattaatt tcctcgtgcg tcatgtaata atgacgcttg atgtgctcga agtttaccgt
       61 ctcggcgatg cgcggctgct gatacaaatc catcaggtag ccctggaggc tcgggtaatc
      121 gagaactcgg cggatgttgc atttgaagtg gccgtgataa acgacgtcga accgcacgag
      181 cgtgcaaaag agtcgccaat cggcttcgac gatgcggttg ccgaacagat agcgactttt
      241 cgttaagcgc tgctcaagct ggtcgagcgc ctcgaatagt ttgcggcagg agatttcata
      301 cggccgctgg cgcgtggcga agccggcctt gtagacgccg ttgttcacgt tgtcgtagag
      361 gaaatcgcag agctggttgt gctcggcttc gatctcctgc gggaaaaaat cgagatcttt
      421 atttcgggca aacgcgtcga agacattgtt gaacatccgg cagatgtcgt cctcgcaatt
      481 gttcacgatc ttgcttgtct ccttgtccca aagcacgggc accgtcaccc gctcttcgaa
      541 atttggatta gtggcggtgt aggcttcgct tagaaaatga aatccgttga ctggatcggt
      601 cgaggtgaat gagtcgttag gcggaaattt tcccgaagac tgacgaaagg cccagccttt
      661 ctcgtcacgg attggatcga caatcgtcat gccgatcgcg ttttcgagac ctttgagttt
      721 tcggagaatg atcgtgcgac tcgcccacgg acacgcgagc gacacgtaaa gatgatatcg
      781 gccggcggcc gcgggataaa ctgtcgatcc atcgtttgag acccattcgc gaaatgcgtc
      841 ctcctgacgt gcgaattcgc ccgaggcagt ttgttcagcc ggagcatgcg caggcatgag
      901 aagaacgtgg aaggtagaaa ttaggaatta gaaagagtcg atcagaagaa ccgcagtgcc
      961 gtcgagctta ccggttcgaa agttatccag agcggagttc gccgcttcaa gcggaaacag
     1021 ttcggttttt gttttcaccg gcacgcgtag cgcgatctcg agaaattctt cgccgtcgcg
     1081 gcgagtcagg ttcgcgactg aacagatggt gcgctcgttc caaagatcga catacggaaa
     1141 agacgggatg tcgctcatgt gaatgccgcc gcataccacg atgccgccct tggcgagcgc
     1201 ccgcagtgca actggcacga gcgtgccgac cggtgcgaag atgatcgcgg cgtcgagttt
     1261 ctccgggggc agttcgtccg agccgccggc ccagactgcg cctaacgagc gggcagattt
     1321 ctgcccagct ctgtcgcccg ggcgggtgaa agcgaaaacc tctcgcccct gataaacggc
     1381 gatctgtgtg atcagatgcg cggcgttgcc gaaaccgtag atgccgagcc gcttcgcgtc
     1441 gccggtcttg cggtaggaac gatagccgag catccccgcg cacagcagcg gtgccacatc
     1501 gacatcatgg tattcgttag gcaaatgaaa acaaaaccgc gcgtcagcaa cggtgaattc
     1561 ggcgtagccg ccatcaatgg tgtagccggt gaacttggcg cgatcgcaaa gattctcgcg
     1621 cgaagatcga caataaacac aatcaccatc cgtccagccg agccacggga tgccaacgcg
     1681 atcaccggtc ttaaatccgg cgatgtgatc ggcgatttcc tcaatacggc cgacaatctg
     1741 atggccaagg atcaacggta gctttggatt aggcagttcg ccatcgacga tgtgaagatc
     1801 ggtacgacaa accgcacagg tggaaacccg gatgagcacc tggccggtaa ccggttttgg
     1861 tttggccaca tcacgcaaag cgagaggttt tcccggcttg tcgagaacca tcgcacgcac
     1921 gtcgacctac ttaaatgtgt acgcacgttc gtcgcaacag gcgcgagtca agctgaggtt
     1981 ttgctagcgg agtttacaga agctaatgca gcgacgccgc cgtcgagcag tggtattagc
     2041 ccattgctcg ttagacttga cggctttaag ccgtcaagtt tgtcgccgga aaaagcatta
     2101 tcccgggcga cgggaagctt ccgcagcgcc ggtattcagg atctgatgca gcgcttgccg
     2161 aagccgctgt tgaggaatcg gttttcgcag gaccacacta acgtctgcgt gttggtcgac
     2221 gatcgaggct gtctcgctcg atccggtcat gagaatgacc ggctggccac cgcccaattg
     2281 ttttaccgcc gcagcgagct gcaatccagt catgccggcc atcgtttgat cggtgaggag
     2341 aaggtcgaag ttgccttccg tcaattttgt catcgcttcg ccgccactgg ttgcagtcga
     2401 gacatgatgc ccatcggcca cgagatattt agcgacgaca tcgcgcggca ccgtgtcatc
     2461 atcgacgacc agaacacgaa tggagcgatc cagcttggcc gtgtcttcga tcgtgccgtc
     2521 cgatgtttcc gctgcgggca agcgaattcg aaacgtggtg ccgcggccga gcgtgctttc
     2581 aatgtccagg gtgccgttgt ggcgtttgat gatgccgaaa accatggaga gtccaagacc
     2641 ggtgcccttg tcgcccttcg ttgagaagaa cggttcgaga cagcggttgc gcactgcctc
     2701 gctcatgccg ctacctgtat cggcgatctc aaagagaaca tcatcctcgt cgcggcgcga
     2761 gcggagcgta atcgtgccac cctctggcat ggcgtcaacc gcattgaaaa taaggttggt
     2821 cattacctcg cgaagctcgg acggattacc gctgatgagt ggaagctttt cgagatcgaa
     2881 ctcgaccgag atggtgcggc cggaggcgag ggcctgcgtt ttccatttcg gctgggtcag
     2941 cgaggcagat tcctcgatca cttcgttcag atcgagcggg gcaaatacgt cttcgatttc
     3001 gcgaccgcga tagaaatcgc gcaaccgact gacgacgtgc gcggcgtcgc gtccggctgt
     3061 gttcagggtt ttgagataat ccagagtggt ttcgcgatcg tccagcgtgg ccggatcatg
     3121 gataagcaga tcgctgtagc cgatgatcga catgagcgcg ttattgaaat cgtgcacgat
     3181 gcccccggcc atttcgccaa aagcgcgaag acgctcctgc tggacgatct gctgctgggt
     3241 ttgcttgagt tcggccagcg cgcggctgag gtccgcggtg cgcgcggaga ctctttgctc
     3301 gagaagcgca ttgtgctttt gcagttcgag atggagaagg cgagtgttta agaggttgcg
     3361 aattcgcatc agcgcctcgg tcatgtcgaa gggttttggc aggaaatcag tggcgcctgc
     3421 ggccagcgag cggcgctttg tctcgggatt ctggtcggcg gtgagcacga ggacgggcag
     3481 gtagttgtcg ggaggaagtg ccacgcgcat ccactgcatc acgtcaaacc cgcttaagtg
     3541 cggcatgttc aagtcgagca ggagcaaatc gggctgccag tcaaggatgc gctgcagagc
     3601 ctgcctcgaa tcgctgatac tggtgtgatt gagaaaaccg gtgcggttga gcacgctttg
     3661 cagaagacag atgttggata cctcatcatc gacaatcaga attcgggcct gcttcagatc
     3721 gtcctcggtc atcactgaaa catggacttg ttacgccgcg gaaagccggc ggtcgtcgac
     3781 aacgctgagc gttatttcgc agccgccggt tcgcttttca aaatgcggcc aatcgtttca
     3841 atcaacagcg ctggatcgac cggctttgcc aaaaataaca ttccgccgcg ttcgacggca
     3901 cccctgcccg cctcactgtg cgatatgagt gaagtcaaaa atagaattgg agtctgacgt
     3961 agaaccgagt cggcctgaag ttcgcgcgcg acgtcgccgc cgtccttgcc cggcatgtcg
     4021 acatcgagaa gaaccaggtc gggggtaaac gctttggccg cagccaaggc cgcactggaa
     4081 cgattctctt cctggacttg gtaagcgttg gttttttcaa gaatcgtttt gacgaggcgt
     4141 gacagctttg ggtcgtcgtc gacgataaga attttgtgtt tttgcatgcg tattcgtttg
     4201 gcgggtcgat agagcacgag ccgtgcgcga cgttcggtcg agaatatctt tttcggctgc
     4261 gactttccgc ttggtttcag cgcgagcgaa gaggcaaagc aaatagcgcc cgactaccgg
     4321 gaagaatcca cgcgggcggc aggaatactg ctgcatccgt taaggaggcg tatgctgtcc
     4381 agcgacactt caaacacgcg caaaccaggg acgttggcag cgactgctac ggccttgatc
     4441 gcagggcttg ttctcattgg ttggacattc gatgtcgatc ttttgaagcg ggtcgtgccc
     4501 ggtctggtgg cgatgaatcc gatgacggcg gtggcgtttc tgctcggcgc gatctcactt
     4561 tttcttttct cggatgagaa atcggatgga cgcgccagtt ttcatccacc actggcgcgt
     4621 ggatgcgcgt tgcttatcgc gctgattgga gtggcaaaac tggtggcgat tgcccgcggt
     4681 atcgacctcg gcgtcgatcg gtggcttttc tcttcaaaac tgacgctcgg gtttcaagtc
     4741 ccgaacgtga tggcgccc
//
LOCUS       DASTDG010000083         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_523290, whole genome shotgun sequence.
ACCESSION   DASTDG010000083 DASTDG010000000
VERSION     DASTDG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523290"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..635)
                     /locus_tag="VFO30_02580"
     CDS             complement(210..635)
                     /locus_tag="VFO30_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418200.1"
                     /translation="MREFLELLNSRGVEYIIIGAHSLAFHARPRYTGDLDILINPTQK
                     NAARIVDLLNDFGFAASKFNESDFMAPEQMIQLGRAPNRIDLLTSITGVSNAEAFAEK
                     IATELDGIPVFILGKDALIRNKRAVGRPQDITDLDLLES"
     gene            861..1289
                     /locus_tag="VFO30_02585"
     CDS             861..1289
                     /locus_tag="VFO30_02585"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HET9418201.1"
                     /translation="MSTKCPKSPKEMTKGLMYFPRMLDKIRMHARGQLPEEYHKNLGA
                     PRAADGACCNFLRVHYRDLRDRVLQGGSDEEILGWCFEKGRHLNEGDIMIWNCFSSKL
                     GWKDFATPTLEEAKQKHGIAHRTDIETIPDLIDFDEGRQK"
     gene            1437..2327
                     /locus_tag="VFO30_02590"
     CDS             1437..2327
                     /locus_tag="VFO30_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9418202.1"
                     /translation="MALAVSAGGTSAQQTTPVAGSALPFIDSKDQAIPVVGKFVTIFG
                     VKIHYVDVGAGPTVILLHGLADDLGVWQSIIQTLAAKHRVIALDQVGFGRSDKPLLCY
                     QANTFVDFLDGFLNELKIDRASLVGNSLGGWIAAAFELEHPERVERLILSDAAGYAAL
                     AKTMNPRALSALRLASREDIRYLGPLTFHDKRFYEDVGLAFKQRVTAGDSYTVSQLLD
                     SMIRGEDVLDGRLGAIEKPTLIVWGHEDKLIPLSFGERFHKEIAGSKLRVIDNCGHMP
                     HVECPKDFATAVLEFLSDLK"
     gene            2324..>3302
                     /locus_tag="VFO30_02595"
     CDS             2324..>3302
                     /locus_tag="VFO30_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9418203.1"
                     /translation="MKRLCTSGLAGVYLILNAWRCDAQQVPAAPTSPSTPSPTATPVV
                     FSSQTLADLKRLQQTALNSDYAYRQVAHLANNIGPRLTGSAQAAKAVEYVANELKAIG
                     CDVQLERVVVPHWVRGGETAALVQFPGMAENTTQKIVVCALGGSVATPPDGITAEMIA
                     VKNFDELKSLGRDKVAGKIVLFNYPFDKEMAAQGRGGEAYGEAVIYRGDGPSAAARQG
                     AVACLVRSVGGADYRLPHTGQTNYANDVPKIPAAAVAAEDAELIVDLVRQGPVKMNLV
                     LTPQTLPDPESANVIGDIKGSEHPEQVVIVSGHLDSWDLGTGAIDDGAGV"
BASE COUNT          723 a          894 c          975 g          710 t
ORIGIN      
        1 accggcttga ccaacgacct gtgggtgcga gtagcacaac atcgcaccgg tgaaatgccg
       61 ggttttcacg cgtgactaca ctgcgctgcg ctcgtttact gctctcggtt tggcgacatc
      121 attccgctca gggatgacaa ctccaagctg ggcattccgg cattagacgt cgcagcgaat
      181 atttggcggc cctcgatgga ttctgccgat cagctctcca gcagatcgag gtcagtaata
      241 tcctgcggtc gaccgacggc tcgcttgttt cggatgagcg cgtctttgcc gagtataaaa
      301 acaggaatgc cgtcgagctc agtcgcaatc ttttcggcga acgcctcggc gttgctcacg
      361 ccggtgatac tggtgagcag gtcaattcgg ttcggcgcgc gtccgagttg aatcatctgc
      421 tcgggtgcca tgaagtccga ctcgttaaac tttgaagctg caaacccgaa gtcgtttaga
      481 agatcgacaa tcctggccgc attcttctgc gtcgggttga tcaggatgtc caagtccccg
      541 gtataacgcg gccgcgcgtg aaacgcgagg ctgtgcgcgc cgataataat gtactcaacg
      601 ccgcgcgagt ttaacaattc gagaaactct ctcaagtcgc tgctcaggtg catggttgag
      661 tagttggacg agaattttca gccgctcagc cccggacagg tcgttgtaaa attcgcggtc
      721 agctttttct gctttggcga agctggtgaa cttctgagcg ttttttcgca caagcaaatt
      781 atacctgctc ccgctgagtt gccgagccgt tttctcggca ccgcgattgc atagccacgg
      841 atgcacgatt agttctgagc atgagcacga aatgtccaaa gagcccgaag gagatgacga
      901 aaggccttat gtattttccg cgcatgctcg acaaaatccg gatgcatgcg cgcggccagt
      961 tgccggagga atatcacaag aacctcggcg cgccgcgagc ggcggacggc gcgtgctgca
     1021 atttcctgcg tgttcattat cgcgacttgc gcgaccgcgt gctgcaaggc ggcagcgacg
     1081 aggagattct cgggtggtgc ttcgaaaaag gacgccacct aaacgaaggc gacatcatga
     1141 tttggaattg cttcagttca aaacttggct ggaaagattt cgccacccca acgctggagg
     1201 aagccaagca gaaacacgga atcgcccatc gcactgacat cgaaacgatt cccgatctga
     1261 tcgatttcga tgaaggtcgc caaaagtgac aagtgactgg tgacccggct tcgctgagct
     1321 tcggtatgac aggtgggtga gagacggctt cttgccattc cgagcgagtt gccagtccag
     1381 cccggaccta ggagtctctc accatgtgtc cttctcgctc gagcatcgtt gtggcgatgg
     1441 ccttggccgt aagtgccggg ggaacgtctg cgcaacagac aacgcctgtc gcaggatcgg
     1501 cattgccgtt catcgactcg aaagaccagg cgattcccgt agtcgggaaa ttcgtcacca
     1561 tctttggcgt gaaaattcac tatgtcgatg ttggagctgg tccgaccgta atcttgctgc
     1621 acggtttggc tgatgacctc ggagtttggc aatcgattat acagacgctt gcggcgaaac
     1681 atcgcgttat cgcgctcgat caggtgggat tcggccgctc ggacaaaccg ttgctgtgtt
     1741 atcaagccaa cacattcgtc gacttcctcg acggattcct taacgagctg aaaattgatc
     1801 gcgcatcgct ggtcggcaat tcactcggtg gctggatcgc ggcagctttc gaattggagc
     1861 atccggagcg agtcgaacgc cttatccttt ctgatgcagc aggttacgcg gctttggcaa
     1921 aaaccatgaa tccgcgcgcc ttgagcgcgc tgcgattagc cagccgcgag gatattcgat
     1981 atctcggccc gctgacgttt cacgataaac gcttctatga agatgtcggt ctggcgttta
     2041 aacagcgagt gaccgccggc gacagctaca cggtcagcca actgctcgac tcgatgatcc
     2101 gcggcgagga cgttctcgac ggccggcttg gcgcgattga gaagccgacc ctcattgttt
     2161 gggggcatga ggacaaactc attccgctca gttttggtga acggtttcat aaggagatcg
     2221 cgggttctaa acttcgcgtc atcgataatt gcggccacat gccgcacgtg gaatgcccca
     2281 aggattttgc caccgctgtc ctcgaatttc tcagcgactt aaaatgaaac gtctttgcac
     2341 aagcgggctt gccggagtct atctaattct caacgcgtgg cggtgcgatg cgcaacaagt
     2401 accggctgcg ccaacatctc cttctacgcc ctcaccgacg gcaacgccgg tggtgttttc
     2461 atcgcaaacg ctggccgatt tgaaacggct gcagcaaaca gcgttgaaca gcgattacgc
     2521 gtatcgccag gtcgcgcatc tcgcgaataa cattgggccg cgattaactg gatcggcgca
     2581 agcggcaaaa gcggtcgagt acgtcgcgaa cgagctgaag gcaattggat gcgacgtgca
     2641 gctcgagaga gtggtggtgc cgcactgggt gcgcggcgga gaaacggcgg cgctcgtgca
     2701 gtttcccggg atggctgaaa acacgacgca gaaaatcgtc gtgtgcgcgc tcggcggaag
     2761 tgtggcaacg ccgcccgacg gaatcacagc cgaaatgatc gcggttaaaa atttcgacga
     2821 actcaaatcg ttaggccgtg acaaggtcgc gggcaaaatc gtgctcttta attatccatt
     2881 cgacaaagaa atggcggcgc aaggtcgggg cggcgaggcg tacggcgaag cggtcattta
     2941 tcggggcgac ggccccagtg cagcggcgcg acaaggcgcg gtggcgtgtt tggtccgttc
     3001 ggtcggtggc gccgactacc gcctgccgca cactggccag acaaactacg caaacgatgt
     3061 gccaaaaatt ccagcggcgg cggtcgcggc cgaagacgca gagttgattg tcgatcttgt
     3121 gcggcaaggt ccggtgaaaa tgaacctggt attaaccccg caaactttac cggacccgga
     3181 gagtgcaaac gtgatcggcg acattaaagg gagcgagcac cccgagcagg tcgtgatcgt
     3241 ctctggccat cttgattcat gggacctcgg caccggcgcg atcgatgatg gcgcgggcgt
     3301 gg
//
LOCUS       DASTDG010000084         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1040948, whole genome shotgun sequence.
ACCESSION   DASTDG010000084 DASTDG010000000
VERSION     DASTDG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040948"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFO30_02600"
     CDS             complement(<1..342)
                     /locus_tag="VFO30_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418204.1"
                     /translation="MALQLFRTKSPDHLMREAAAPERLMKRTLGALDLTAIGIGAIIG
                     AGIFALTGTAAAGQTFASRLETPVINFIQAWFSGGNVVFGRAGAGPAIAVSFIVAGIA
                     CGFAALCYAELA"
     gene            466..675
                     /locus_tag="VFO30_02605"
     CDS             466..675
                     /locus_tag="VFO30_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418205.1"
                     /translation="MFDFSPANLLAGLLFGSIGFVAFIYGKRMNLWKPMFCGITLMIY
                     PYFVSNIWFVWLIGGVGTVALFFLR"
     gene            711..1805
                     /locus_tag="VFO30_02610"
     CDS             711..1805
                     /locus_tag="VFO30_02610"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HET9418206.1"
                     /translation="MADEAEKIILADTAVSAIDRSRRGFFRRRKIQQSPPPTHCENCR
                     GLLHGHWCAQCGQAAIDYRRSFRHVIGDVLDSFLNWDSKFFATIGLLIAKPWSLTNAF
                     LSGKRVRYVHPLRLYLLASILFFFAVNYWARAIHVDPSQLGPKDRAQIEAELQNKDLP
                     PKVREQLQAILREPLPTPAAESEAPRMDAGPKERPFLSFDDGTKAETPLERWLELRAK
                     EKFGEHGTRFALFIKALVSNLPYMMLCCIPLFAFVLKILYLRRRIFYIDHLVYALHIH
                     TFAYMGVMLIVLVTIGLNHSASGRHDGWLIALLWCAFVAQIFLSIRRVYRQSWFISVL
                     KFLFGGLAYLIVLSVALAVTFFITLALPTS"
     gene            complement(1780..>3389)
                     /locus_tag="VFO30_02615"
     CDS             complement(1780..>3389)
                     /locus_tag="VFO30_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9418207.1"
                     /translation="GNWRSEFERLEIPIPQFFGEAAFELYDTYGFPLDLTELMARERG
                     LTVDVAGFEKLMEEQRTRARAARKKDAIMVEAETFRASPTNFLGYDFFETEGVVEAVL
                     PGNKPNEFAVVLDRTPCYAEMGGQVGDRGLLHVPGRDRTEVGQLRITDTQKRGDIFVH
                     RTRLVDGRAPEPGEAVRIAVDIDRRKLIQGHHTATHILHWALHELVSRDAKQKGSYVG
                     PDKLTFDFSSAALAKETVRKIEKLVNAKMRENAPVSWTQIPYDEAKKRKDIQQFFGEK
                     YGDIVRVVQIGGTPNKLNGYSMELCGGTHVRSTSEIGAFRIVREEAIAAGIRRIEAVA
                     GEAAREWAKAEARRQRERFEILRRKKPDIAALPPLDENAEIAATFDQIDIRAAHLEKL
                     DVDLRDWEKKQAKAGEAQLKSRAAAIAKELEAQHRGQKSLVAEVPDADGDLLQAIADA
                     LKGKVKGPIVLASAMNRSVALVASVPKEQTATIRADKLIQQLAPIVGGKGGGRPENAR
                     GAGKDVDKIGMLIAEARRLLSSSAERA"
BASE COUNT          642 a         1042 c          913 g          792 t
ORIGIN      
        1 cgcgagctcg gcataacaga gcgccgcgaa accgcaggca atgcctgcca cgatgaacga
       61 aaccgcaatc gccggcccgg caccggcgcg accgaagaca acattgccac ccgagaacca
      121 cgcctgaata aaattgatga cgggcgtctc gagccgcgat gcaaatgtct gccccgcagc
      181 ggccgttccg gtaagcgcga aaattcccgc gccgatgatc gcgccgatgc caattgcagt
      241 gaggtcaagc gcgccgagcg tgcgcttcat cagccgctcc ggcgcggctg cttcccgcat
      301 caggtgatcg gggcttttgg tgcgaaaaag ctgtaaagcc attgctatgc gctaaacatt
      361 cgattagcca ccggcggtcg gacggatgct gacatttctg ccgcgccagg gtcaacgcga
      421 agtttcgatc acgtcgttgg cgcgccagta gctgcgaaga tcgacatgtt cgattttagc
      481 ccggccaatt tgctcgccgg cttgcttttc ggctccatcg ggttcgttgc atttatctac
      541 ggcaaacgca tgaacctgtg gaagccgatg ttttgcggga ttacgctgat gatttatcca
      601 tatttcgtca gcaacatctg gttcgtctgg ctaatcggcg gcgtcggcac ggtggcgttg
      661 ttctttctgc gatagacgcc catttgcgct tttcataagc tcggcctgcg atggccgacg
      721 aagcggagaa gatcatcctc gccgataccg ccgtttcagc catcgaccgg tcgcgacgcg
      781 gcttttttcg ccgtcgcaaa attcaacaat caccgccgcc gacgcactgc gagaactgcc
      841 gcgggttact gcacgggcat tggtgcgcgc agtgcgggca ggcggcgatc gattaccggc
      901 gatcctttcg tcatgtcatc ggcgacgtgc tcgattcgtt tctcaattgg gactcgaagt
      961 tctttgctac catcggcctt ctcatcgcga agccatggag cctaacaaac gcattccttt
     1021 ccggaaaacg cgttcgctac gtccatccgc tgcggcttta tctcctcgcc agcattctgt
     1081 tcttctttgc ggtgaactac tgggcgcgcg cgattcatgt cgacccgagc cagcttggac
     1141 caaaagaccg cgcccaaatc gaggctgaac tgcaaaataa agatctgccg cccaaagtgc
     1201 gtgagcagtt gcaggcgatt ttgcgcgaac cattgcccac gcccgcggcg gaaagcgagg
     1261 cgccccgcat ggacgcaggc ccaaaggagc ggccgtttct ttcttttgat gacggaacga
     1321 aagcagaaac gccgctcgag cgctggttgg aactgcgcgc gaaggagaaa ttcggcgagc
     1381 atggcacaag gttcgcgctg tttatcaaag ccctggtcag caacctgcct tacatgatgc
     1441 tctgctgtat cccgttgttc gcgttcgtgc tcaagattct ttatctgcga cgccgcatct
     1501 tctacatcga ccatctcgtt tatgcgctgc acattcatac ttttgcttac atgggtgtga
     1561 tgctgatcgt gctggtgacg attggcttga accactcggc ttcaggccga cacgacggct
     1621 ggctgatcgc gctgctttgg tgcgcatttg tcgcgcagat ttttctctcg atccgccgcg
     1681 tctatcggca gagctggttt atcagcgtgc tgaaatttct cttcggcggt ctcgcttacc
     1741 tgatcgtgct gtcggtcgca ctcgcggtga cgttcttcat cacgctcgct ctgccgacga
     1801 gctaagcaac cgccgcgctt cggcgatcaa catgccgatc ttgtccacgt ctttgcccgc
     1861 accgcgcgca ttctccggtc gcccaccacc ttttccaccg acgattggcg cgagctgctg
     1921 aatcaatttg tccgcacgaa tggttgcagt ctgctccttc ggaacagacg ctactagtgc
     1981 aactgagcgg ttcatcgcgc tggcgagaac gattggaccc ttcacttttc ccttcagcgc
     2041 atcggcgatt gcttgcagga gatcgccgtc ggcatccggc acttcggcga cgagggactt
     2101 ctggccgcga tgctgggcct caagctcttt cgcgatggcg gcggcacgac tcttcagttg
     2161 tgcttcgcca gccttcgcct gttttttttc ccaatcgcga agatcaacat cgagcttttc
     2221 cagatgcgcc gcacggatgt cgatctggtc gaacgttgcg gcaatctcag cgttttcatc
     2281 gagcggcggt aacgctgcaa tgtccggttt cttgcgccgc aaaatctcaa atcgctcccg
     2341 ctgccgtcgc gcttcggcct tcgcccactc gcgcgcggct tcacccgcta ccgcctcgat
     2401 gcgccgaatg ccggcagcga ttgcctcttc cctcacgatg cgaaacgcgc cgatctccga
     2461 tgtcgatctt acgtgcgtgc cgccgcacaa ttccatcgaa taaccattca acttgttagg
     2521 cgtgccaccg atctgcacca cgcgaacgat gtctccgtat ttctcgccaa aaaattgctg
     2581 gatgtctttt cgtttcttcg cttcatcgta cggaatttgt gtccacgaaa ccggcgcgtt
     2641 ctcgcgcatc ttcgcgttca caagcttctc gatctttcga acggtttcct ttgccaacgc
     2701 cgcactggaa aaatcgaacg tgagtttgtc cggcccgaca tagctgccct tctgcttcgc
     2761 gtcgcggctg acgagctcgt gcaatgccca atgcaaaatg tgcgtagcgg tgtgatgtcc
     2821 ttggatgagc ttgcgtcgat cgatatcaac tgcgatgcgc accgcttcgc ccggttccgg
     2881 cgcacggccg tcgaccagcc gcgtgcgatg gacaaaaata tcgccccgct tttgtgtgtc
     2941 ggtgatgcga agctgaccga cttcagttcg gtcacgtcca ggcacatgca gcaatccgcg
     3001 gtcaccgacc tgtccgccca tttcagcgta gcaaggtgtg cgatcaagga ccacggcgaa
     3061 ttcattcggt ttgttgccag gcagcaccgc ttcgaccacg ccttctgttt cgaaaaaatc
     3121 gtagccgaga aagttggttg gagaagcgcg gaacgtttcc gcttcaacca tgatcgcgtc
     3181 tttcttgcgc gcggcacgcg cacgcgtgcg ctgctcctcc atcagcttct caaagcctgc
     3241 gacgtcaacg gtgagaccgc gctcgcgtgc catcagctcg gtcaaatcga gcggaaaccc
     3301 gtaagtatcg taaagctcaa acgcagcttc tccgaaaaac tgcgggattg gaatttcgag
     3361 gcgttcgaac tcggatcgcc aattccctt
//
LOCUS       DASTDG010000085         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_19881, whole genome shotgun sequence.
ACCESSION   DASTDG010000085 DASTDG010000000
VERSION     DASTDG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19881"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFO30_02620"
     CDS             <1..1037
                     /locus_tag="VFO30_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9418208.1"
                     /translation="LLASLLQANVCNLSLGDAMLTPTRFSYAVLAFTIVLAGVLHLGA
                     PLLVILFSYFALSQLHSQAKRKWVALLLFVLLVAGIAAAAVYFTREAIRALPDVADTS
                     IPSASAWAQKRQIELPFTDFESLRAAVIRALKEEASYLQSVAHFAGHTASALVFSLIA
                     IVAAASLFLTAGFGPQQSADGVRTNLYSVVTREVTTRFRDFYQSFATVIGAQLMISLI
                     NTALTSVFLIAVRLPYAPLLIGVTFLCGLIPIVGNLVSNSVIVFVALSVSLKLAIAAL
                     IFLVVIHKLEYVLNSKIIGGRIRNPVWLTLIALVIGERLMGIPGLILAPVVLNYFRVE
                     MLKIQVPADT"
     gene            complement(1047..1631)
                     /locus_tag="VFO30_02625"
     CDS             complement(1047..1631)
                     /locus_tag="VFO30_02625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET9418209.1"
                     /translation="MKSPLVLASNSPRRRELLAAAGFEFQVVAPNITERHDVDLTARE
                     LTSLNATRKALAVARRCFGRIVLAADTVVVLDADILGKPQSLGEAAAMLRRLSGRAHE
                     VCSAVFICDPVRRRWTTFQDVSRVDFRRLTDARIRAYIKSVDVLDKAGAYAAQERADE
                     IIERIEGSYTNVIGLPMERTVAALAQFGIRPSEV"
     gene            complement(1652..2362)
                     /locus_tag="VFO30_02630"
     CDS             complement(1652..2362)
                     /locus_tag="VFO30_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HET9418210.1"
                     /translation="MLELVFTLGLIASTRADLTLVQKVEGAGQGATEMTIKIKGDKAR
                     IDASSKLTTIIDSKTGEIANLMNDRKTVMRISAEKMRAAAEMIGKFNANATSASKGKL
                     TPTGKSEKIAGYDVEEYVYDTPNFKATYWIAPNYPDGAAILKELQSLSSDLWQTNNFR
                     LPDYRDFPGLPVKTVVSAGDSNVTTTLVSVSKAPLDDAQFAVPKDFKEIKVPEMHFVP
                     PGEKAAEEKPGASASPKP"
     gene            2822..3487
                     /locus_tag="VFO30_02635"
     CDS             2822..3487
                     /locus_tag="VFO30_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418211.1"
                     /translation="MAACQRFGPAPYNPRIDPSNFVSRVTNPYFPLPPGTTFVYEGQT
                     ADGFEHDEFAVTHRTRTILGVECVEVHDTVRTDGQLTEDTLDWFAQDKAGNVWYFGEN
                     THELEDGLITTIEGSFVAGVDRAKPGIIMRAHPFVGDFYRQEFALANAEDYAETVGLN
                     ATVKLANGRTYTNCLKSKETTPLEPDLREFKFYATGVGNVLEVDAVTGDRLELVRIKS
                     NSE"
     gene            3563..3636
                     /locus_tag="VFO30_02640"
     tRNA            3563..3636
                     /locus_tag="VFO30_02640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3595..3597,aa:Gly,seq:tcc)
     gene            complement(3753..4214)
                     /locus_tag="VFO30_02645"
     CDS             complement(3753..4214)
                     /locus_tag="VFO30_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418212.1"
                     /translation="MKKDLLAASLLLSFGLSTASGGQSDPRFYGVWVGEETHEIPARP
                     PSPLHAGQTGVAPVKKSAVLLIGDYGKTLAFGQGRVLVKDEVTAWWRKNRLDWRQYLA
                     YSGRDHGTLVLSADGNTLTENAVAILPGNPGPVTCNITGTFHREKREKIGR"
     gene            complement(4240..4818)
                     /gene="pgsA"
                     /locus_tag="VFO30_02650"
     CDS             complement(4240..4818)
                     /gene="pgsA"
                     /locus_tag="VFO30_02650"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9418213.1"
                     /translation="MNWANRLTLSRLGLTVLFVFALNSSWPYARTSALVIFLIAGLTD
                     FIDGEIARRYKWVTNFGKLMDPLVDKIMIAAAFISLVPLKAVPAWAATTVVARDFLIT
                     GLRMMASAKGRVLPAERLGKQKTSWQVVTVIFFLALLSLHELRYIDEKSSWWFGTWHE
                     AGRVLVWITVALTIYSGIGYAWRHRELISHDH"
     gene            complement(4815..>5206)
                     /locus_tag="VFO30_02655"
     CDS             complement(4815..>5206)
                     /locus_tag="VFO30_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9418214.1"
                     /translation="KLEDGREIEGIDYEAHGAMAEHQLAMIAEQAAARFELRRVIIRH
                     RVGFVAVGEASLFLQVAAGHRGAAFEASKWIVDELKLKVPIWKHPRLRQGYGAAGPPS
                     QNSGAESNPLARVADAGPRSPTAATVP"
BASE COUNT         1074 a         1556 c         1434 g         1142 t
ORIGIN      
        1 tcttgctcgc gtcgttgctg caagcgaacg tatgcaacct ctcgctcggc gacgcaatgc
       61 tcacaccgac tcggttctcc tatgccgtcc tggcatttac tattgtcctt gccggagtct
      121 tgcatttggg tgcgccgctg ctggtcatcc tgttctccta cttcgcgctt tctcagcttc
      181 attctcaagc caagcgcaag tgggtcgcac tgctcctatt cgtccttctc gtcgcgggaa
      241 ttgcggcggc ggcggtctat tttacgcgcg aggctattcg tgccttgccc gacgtcgcgg
      301 acacctcgat tccgtccgct agcgcgtggg cgcagaagcg ccagatcgag ctgccgttta
      361 ccgatttcga aagcctgcgc gcggccgtca tccgggcgct gaaggaggag gcgtcgtact
      421 tgcaaagtgt cgcgcacttt gccggccaca ccgcgagcgc actggtcttc agtctcatcg
      481 caatcgtcgc tgcggcgagt ctgtttttaa cggcaggttt cggtcctcag cagagcgcag
      541 atggcgtgcg tacgaatcta tactctgtcg tgacgcgcga ggtgacgact cggtttcgcg
      601 acttctacca aagcttcgct accgtcatcg gagcgcagct aatgatctcg ttgatcaaca
      661 cagcgctgac gagcgtattt ctcatcgctg tgcgtctacc atacgcgccg ctactgatcg
      721 gcgtgacttt cctctgcggg ctgatcccga tcgtcggaaa cctggtgagt aactctgtca
      781 tcgttttcgt tgccctgtcc gtgtcgttaa agctcgccat tgcggcactt atttttctcg
      841 tcgtcattca caagctcgag tacgtcttga acagcaaaat tatcggtgga cgtattcgca
      901 atccggtttg gctgacactg attgcgctgg ttatcggcga aagactgatg ggaattccgg
      961 gactgatcct tgcgccggtg gtgttaaact acttccgcgt cgagatgctt aagatccagg
     1021 tgccggcaga cacctagttt ctttttttac acctcacttg gacgtatccc aaactgggcc
     1081 agcgcggcca ccgttctctc catgggcaga ccgatgacat tcgtgtacga gccctcaatt
     1141 cgttcgatga tttcatcggc gcgttcttgt gcggcatacg cgccagcctt gtcgagcaca
     1201 tccacgctct tgatgtaagc gcggatgcgg gcgtcggtca gccgacgaaa gtcgacgcgt
     1261 gacacgtctt gaaacgttgt ccaccgtcgg cgcactgggt cacagataaa gacagcagaa
     1321 cagacttcgt gcgctcggcc gctcaaacgc cgcagcatcg cggctgcttc accaagactc
     1381 tgtggcttcc cgagaatgtc ggcgtctagc acaactaccg tgtcggccgc gagcacaatc
     1441 cttccaaaac aacgccgcgc aaccgctaaa gcctttcgcg tcgcattcaa actcgtcagc
     1501 tcgcgcgccg taagatcgac atcgtgacgc tcagtgatat tcggcgcgac aacctggaat
     1561 tcgaaaccgg cagcggcgag cagttcacgg cgccgcggcg aattggaagc caataccagc
     1621 ggcgacttca ccgcttgaac gagcaattgc cctacggttt cggcgaagcg gacgcgcccg
     1681 gcttctcttc cgccgctttt tcacccggcg ggacgaaatg catttccggg actttgatct
     1741 ccttgaaatc ttttggaacc gcaaactgcg cgtcatcgag cggcgcctta ctcacggaga
     1801 caagcgtcgt cgtcacattg ctgtcgccag cggacacgac tgttttcact ggcagccccg
     1861 gaaaatcgcg atagtcaggc agtcggaaat tgttcgtctg ccagagatcg gagctgagtg
     1921 attgcagttc tttgagaatc gcggcgccat cggggtagtt cggcgcgatc cagtaggtcg
     1981 ccttgaaatt tggcgtgtcg taaacgtatt cctcaacgtc gtagccggct atcttctcac
     2041 ttttgcccgt cggcgtcagt ttgcctttgc tggcactggt cgcgttcgcg ttgaatttcc
     2101 caatcatctc agcagcggcg cgcatctttt ctgccgagat gcgcattacc gtcttacgat
     2161 cgttcatcag gttcgcaatc tcgccggtct tactgtcgat gatagtcgta agcttggacg
     2221 atgcgtcaat ccgcgctttg tcgcctttga tcttgatcgt catctcggtg gcgccctgac
     2281 ccgcgccttc gactttttgt acgagtgtaa gatcggctcg cgtcgaagcg atcaggccga
     2341 gcgtgaaaac caactccaac ataacggatt tcatcacggc agtagctaga ctgcggccgc
     2401 tcaaatagca agtgacaccc ggacggtatt caagtccgga acgatcaatg aaagggttac
     2461 ccgtcgaaga acgggacgaa taagcttgca attttataga atgtctaaaa acctcagtgt
     2521 tatgaaaccg attgggtgcg ctcgcgccat tggaatgccg attcacctcg agttcgcttt
     2581 gcctgttggc ctgctcagtc tggtccttgt cgctgcggcc cacggtcaaa ttaacgcctg
     2641 ccagacgacc gccgacgacc tcttgactgc ctgccgacgc gcagcacaga gcaacaatga
     2701 ggtagcttta gctaattgcg aaaacgttcc caacgctgac gcacaacaaa gatgccgaga
     2761 caaagcggcc gcagatttaa aggacaccca ggacacttgt agcgacgagt acgatgtgcg
     2821 cgtggctgcc tgccagcgtt tcgggcccgc tccctacaat ccgcggatcg acccgtccaa
     2881 ttttgtcagc cgcgttacga atccctactt tccgttgccg ccgggcacga ccttcgttta
     2941 cgaaggacag accgcggacg gtttcgagca cgacgagttc gcggtgaccc accgaacgcg
     3001 gacgatcctt ggcgtcgaat gcgtggaagt tcacgatacc gtgcggaccg acggccagct
     3061 cactgaggac accctcgact ggtttgcgca agataaggcc ggcaacgttt ggtatttcgg
     3121 cgaaaacacg catgagctcg aagacggtct gatcaccact atcgaaggct cattcgtggc
     3181 tggggtcgac cgcgccaagc cgggaatcat catgagagct catccattcg tcggcgattt
     3241 ttatcggcaa gagttcgcgc tcgccaacgc tgaagactac gccgaaaccg tcggtttgaa
     3301 tgcgacggta aagctagcta acggccgcac ctacacgaac tgcctgaaat caaaagaaac
     3361 cacgcctttg gaacctgatc tgcgcgagtt caagttctac gccacggggg tcggcaacgt
     3421 tctggaggtc gatgccgtca caggcgaccg gcttgaactg gtgcggatca aaagcaattc
     3481 tgagtagcgg gcggccacac aaattgcttg gcagcatcgc gtccacacgg caggttagtc
     3541 tgagccagac tggcacttct acgcgggtgt agcttaatgg taaagctgta gccttccaag
     3601 ctactgacga gggttcgatt cccttcaccc gctccagcct tcgcttgagt ccgcgaagcc
     3661 gaggctggag cgagcgcgcc gtaggctggg cgcaaggcgg gcggccgcga cagacaagct
     3721 acggctggca ggccagcgtc ccgcttgtaa aattaacgtc ctattttttc tctcttttcg
     3781 cgatgaaacg tgccagtaat gttgcaggta accgggccgg ggtttccagg cagaatagcg
     3841 actgcatttt ccgtcaaagt atttccgtct gctgacagca ctagcgtgcc atggtcgcgt
     3901 cccgaatagg cgaggtattg tctccagtct aacctgtttt tgcgccacca cgcagtaacc
     3961 tcatccttca ccagcacgcg gccttgtcca aaggccaaag tcttcccata atcaccaatc
     4021 agaagaaccg cggatttttt gacaggtgcc acccctgttt ggccggcgtg gagcggtgag
     4081 gggggacgag cgggtatttc atgagtttcc tccccaaccc acacaccgta aaaccgcgga
     4141 tcggactgcc cgccggatgc ggtcgacagc ccaaaactaa gcagaagact ggcggccagc
     4201 aggtcttttt tcatcgggca agctgtgcgt tacacacgtt tagtgatcgt gtgaaatcag
     4261 ctcgcgatgc cgccaggcgt aaccgatgcc ggagtaaatc gttagcgcga cggtaatcca
     4321 aaccagcacg cgaccggctt cgtgccacgt gccaaaccac cagctcgatt tttcgtcgat
     4381 gtagcgcagt tcatgaagcg aaagcaaggc gagaaagaaa atcaccgtca ccacttgcca
     4441 tgaggtcttc tgtttcccga gccgctcggc cgggagcacg cgacccttgg cactcgccat
     4501 catccgcaaa ccggtgatga gaaaatcgcg cgctaccacc gtggtcgcgg cccatgccgg
     4561 gaccgccttc aacggcacca gcgaaatgaa cgccgccgcg atcatgattt tgtcgaccaa
     4621 cgggtccatt aatttgccga agtttgtcac ccacttgtaa cgccgcgcga tttcgccatc
     4681 gatgaaatcg gtcagcccgg cgatgaggaa aataaccagc gccgaggtgc gcgcgtacgg
     4741 ccaggacgaa ttgagcgcga aaacgaagag caccgtcaaa cccagtcggc tcagcgtgag
     4801 acgattcgcc cagttcatgg cactgtagcc gcggtcggcg accgcggacc agcgtcagcg
     4861 acgcgggcta aaggattgct ttccgcgccc gagttctgcg aaggcgggcc tgccgcgccg
     4921 tagccttggc gaaggcgggg gtgtttccag attggaactt tcagcttcaa ctcatcgacg
     4981 atccatttgc tcgcctcgaa cgcggcgccg cggtgcccgg ccgcgacttg caggaagagc
     5041 gaagcttcac cgacggcaac aaagccgacg cgatgtcgga tgatgacgcg gcgcaattca
     5101 aaacgcgcag ctgcttgttc cgcaatcatc gccagctggt gctccgccat cgcgccatgc
     5161 gcctcgtaat cgattccctc gatttcgcgg ccgtcctcca gcttcc
//
LOCUS       DASTDG010000086         7477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_991724, whole genome shotgun sequence.
ACCESSION   DASTDG010000086 DASTDG010000000
VERSION     DASTDG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991724"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFO30_02660"
     CDS             complement(<1..1223)
                     /locus_tag="VFO30_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9418215.1"
                     /translation="MSNLTLQTRGARRALIVKSVLFVCTGNICRSPIAEGLFRALIGN
                     RKDIEVASAGVHAVRGQPPSLYAVQVCEDEGVDITGLRSQPLTASLVDRATHIFAMTG
                     AHLETIQMLFPHGGEKSFLLREFEEPGTTVWRDVPDPIGLGRDVYEDCARTIKNALPS
                     VLAFVEQSALVPPGAARTGGAYVISHTAHPMEPARSTSSSRVYGEALRRTDPEIYDAI
                     MAEEKRQRENIELIASENFTSRAVMEAQGSVMTNKYAEGYPRKRWYGGCENVDVAEQL
                     AIDRAKRLFGCEHVNVQPHSGAQANMAVYFAAIKPGDKILSMNLAHGGHLTHGHPASF
                     SGKLYQVTQYGVDEKTEMIDYNALQKQAEEVRPVMITAGASAYPRIIDFPRLRQIADS
                     VGAILFIDMAHIAGLV"
     gene            1292..2017
                     /locus_tag="VFO30_02665"
     CDS             1292..2017
                     /locus_tag="VFO30_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9418216.1"
                     /translation="MHVLKNLVRGAAAKFDYQIARRADAFGDMRRFVPASTRPVILDI
                     GANLGQTAKKFRATFPSSAIHCFEPGTETFKALRQNMAGANDVHLWHCAVGSSVGKQT
                     FFENTEPDMSSFLELSATGWGEVKNYPIVNVTTVDQFLTEQSIAAVDILKSDTQGYEL
                     EVFKGAEEAMRRGRIGLVYFEFIFSEMYRSLPRFDEVFRFLTDRDFLLVSIYDIHHQR
                     NLADWANLLFVNREYHRRSGAAA"
     gene            2014..3060
                     /locus_tag="VFO30_02670"
     CDS             2014..3060
                     /locus_tag="VFO30_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418217.1"
                     /translation="MTGMRRRWKWLRYRLEWLGLLLATKLIPLLPHRVCLRLAHSVGW
                     LMSIFDRHGRAVALSNLEVAFGEELSTLQRRKIMRESYQQFARTMSDFLWSPRLTRQN
                     FSVYIELENFEEALEAGGAERSIMIACFHYSNFEWLSLACGYLGLPGTIIMERLKNSL
                     LDPIFKRLREQSGHALIPRERGIIRLYKVLRRKGRTALLVDLTVPPAQGAVVIECFGL
                     KTCVTSAHAWLHERTGVSIIPAHCEPAPEGRYRLVFHRKIDNVQGMTHQQIAQACWDS
                     FEPYVRKNPAPWLWMYKHWRYKPADARRRYPFYANVAPRFEKLLHREQRDADHFVDPH
                     RNPLPQGEADAKRP"
     gene            3116..3805
                     /locus_tag="VFO30_02675"
     CDS             3116..3805
                     /locus_tag="VFO30_02675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9418218.1"
                     /translation="MAELLQYLVMRVISPLFFIAALVCATLFTSCETMRLAGGGGAYH
                     VTAYKPHDPSAVRVKVSLSKQNVYVMEGDRCLMSAACSVGLPNKPTPHGNFTIYSKQE
                     HKRSGSYGFSVQGNNVVPTTSGHTSGRYVGYPMGYWCEFAPGYGFHQGFVHPVPRTHG
                     CIRLKGEAAPKFFALVRTGTPVNIATSQPEDATVGPKVQRVDDSRTPDPDPHIMVTDA
                     AFQKPEGQLLE"
     gene            3834..5072
                     /locus_tag="VFO30_02680"
     CDS             3834..5072
                     /locus_tag="VFO30_02680"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9418219.1"
                     /translation="MSTNLISSGTTAREKVNLRTPDVMAAVQEQVESHYRSDIVEKIR
                     RAGGIISVGDTTVRLAKQFGFCYGVERAIDLAYAARKVFKDRRLFIVGEIIHNPEVNQ
                     QIASLGIKNLTGPNKQADISDLGPEDVVIVPAFGTELSIQQEIKERGCQVVDTTCGDV
                     MSVWKRVRKYASESATSIIHGKAEHEETKATSSRALGDGNGHYLVVLTLADTDYVCDY
                     IRHGGDKQAFFDKFKGAYSPGFDPDVHLKTVGVANQTTMLRGETEEVQRRVRRAIVDR
                     DGPELAEKNFRFFDTICGATQERQDALRELLNVPLDLLLVVGGYNSSNTSHLAEMGEE
                     KLPTYFVLNASRLVSANEIKHYNLHEKREVVSRFWLPSGPAVIGITAGASCPNNLIEE
                     TLIRLFELRGIPRQQLELAA"
     gene            5196..6056
                     /locus_tag="VFO30_02685"
     CDS             5196..6056
                     /locus_tag="VFO30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9418220.1"
                     /translation="MRITIVMRAIWKGSISFGLVNIPIALYPATQKEELKFRLLRKSD
                     LSPINYKRVAEKDGREVPWDEIVKGYEYDKGKYVVLKDEDFQRVDIEATQTVDIQDFV
                     NVDEIDPMFFYKPYYLEPQKGGDKAYVLLRDSLKDSNKVGVAKVVIKTRQYLAGVKPE
                     DGVLVLELMHFAEELADPEKLHVPKKLDAGKREMDMAKALINSMTSKWNPEKYHDDYR
                     EAVMKVIEEKVEHGGEEIEEKPKKAPKPTKVIDLVQVLQKSLEQAGGAKKVSTTKRAA
                     KHPQRHKKAA"
     gene            6164..6610
                     /locus_tag="VFO30_02690"
     CDS             6164..6610
                     /locus_tag="VFO30_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9418221.1"
                     /translation="MNTLNRFADLIYSVFRLIVGLLFACHGGQKLLGFPPGGDGAGQG
                     IFLLGAWIELVGGLLIAFGFLTRLAAFIAAGEMAVAYFMVHAAGKALDHTPSAIEQVF
                     PLLNKGEPAVLYCWCFLFFLFYGAGGWSIDALIRRGRAATLPAATP"
     gene            6630..>7477
                     /locus_tag="VFO30_02695"
     CDS             6630..>7477
                     /locus_tag="VFO30_02695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9418222.1"
                     /translation="MTRGFVAFALLLAAAAGAEVIKDFGRERLNTSPRHAEWVDIKSG
                     TRTIKAFVVYPERKDKAPAVLVISEIFGLTDWVRSLCDELAENGVIAFAPDLHSGQKF
                     EDLDAARKVTSALPKEQVKADLDATSDYALTKIPSCNGTLAVCGFCWGGGVTFQYAAT
                     NPKLKAAYSFYGVAIDNSDDAAKVVCPVYGFYGENDERVNVTIPKAEELMKTAGKKYE
                     PVIYKGAGHGFMRSGEPNNPGVREADRKARDEAWMRWKKLLAELAGSRSGGLRSPSSM
                     LTWAAER"
BASE COUNT         1603 a         2119 c         2212 g         1543 t
ORIGIN      
        1 acgagcccgg cgatgtgcgc catgtcgatg aacaaaatcg ccccgacgga atcggcgatt
       61 tggcggagcc gtgggaaatc gatgatgcgc ggataagccg atgcaccggc ggtgatcatc
      121 accggccgca cttcctccgc ctgtttttgc agcgcgttat aatcgatcat ctccgtcttc
      181 tcatccacgc cgtactgcgt cacctggtac aacttgccgg agaaactcgc cgggtgaccg
      241 tgcgtgagat ggccgccatg cgcgaggttc atgctcagga ttttgtcacc gggtttaatg
      301 gcggcgaaat agaccgccat gtttgcctgc gcgccgctgt gcggctgcac gttgacgtgc
      361 tcgcaaccga acaatcgctt ggcgcgatcg atcgccagtt gctccgcaac atcgacattt
      421 tcgcagccgc cataccaacg tttccgcgga taaccttcgg cgtacttgtt cgtcatcacg
      481 ctgccctgcg cttccataac cgcgcggctg gtgaaatttt ccgaggcgat caactcgatg
      541 ttctcgcgct gccgtttttc ctcggccatg atcgcgtcgt aaatctcggg atcggtgcgg
      601 cgcagtgctt cgccatagac gcgagatgaa gatgtcgatc ttgcgggttc catcgggtga
      661 gcagtatggc tgatcacgta agcgccgcca gtgcgcgccg ctcccggtgg aacaagcgcc
      721 gattgctcga caaacgccag cacactcggc aaagcgtttt tgatcgtgcg ggcgcaatcc
      781 tcgtagacat ctcggcccag gccgatcggg tccggcacgt cgcgccagac ggtcgtgccc
      841 ggctcctcga actcgcggag aagaaaagat ttctcaccgc cgtgggggaa gagcatctgg
      901 atcgtctcca gatgcgcgcc ggtcatggcg aaaatgtgtg tcgcgcggtc gaccaaactc
      961 gccgtcagcg gttggctgcg cagcccggtg atgtccacgc cttcatcttc gcagacctgc
     1021 accgcataca aactcggcgg ctgaccgcgc acggcgtgga caccggccga agcaacttcg
     1081 atgtccttgc gattgccgat cagggcgcga aacagcccct ccgcgatcgg gctgcggcaa
     1141 atgttacccg tgcaaacgaa caggacgctt ttcacgatga gcgcgcggcg cgcaccgcgc
     1201 gtttgcaacg taagattgga caaggtctag tcaatcggcg caaaaattcc gcatctggta
     1261 cctgtaagat cgatagctgc gctggccgaa catgcacgtg ctgaaaaatc tggtccgcgg
     1321 cgccgccgca aaatttgatt accagatcgc gcgccgcgcg gacgcgtttg gcgacatgcg
     1381 gcggttcgtc cctgccagca cgcggccggt gatcctggac atcggcgcta atctcgggca
     1441 gacggcgaag aagttccgcg cgacgtttcc ttccagcgcc atccattgtt tcgagcctgg
     1501 cactgagacg ttcaaagcgc tgcgacaaaa catggcaggc gcaaacgatg ttcatctttg
     1561 gcattgcgcg gtcgggtcat cggttggaaa acagacgttc ttcgagaaca ccgagccgga
     1621 catgagctcg ttcctcgaac tgagcgcgac cggctggggc gaagtgaaaa attatccaat
     1681 tgtcaacgtg acaacggtcg atcagttcct gaccgagcag agcattgccg cggtcgacat
     1741 tttgaaatcg gacacgcaag gctacgagct ggaggttttc aaaggcgccg aggaggcgat
     1801 gcgccgtggt cgcattggac tcgtctattt cgaattcatt ttttccgaga tgtatcgaag
     1861 cctgccgcgc ttcgacgagg tctttcggtt tctgacggat cgcgactttt tgctggtgtc
     1921 gatttacgac atccatcatc agcggaacct cgccgactgg gccaaccttc tctttgtgaa
     1981 tcgcgagtac caccgccgca gcggtgccgc cgcatgacag gaatgcgacg tcgctggaaa
     2041 tggcttcgtt accggctcga atggctgggg ttgcttctcg cgacgaagtt gatcccgtta
     2101 ctcccgcacc gcgtttgttt gcgccttgcc cactccgtcg gctggttaat gtcgattttc
     2161 gatcgtcacg gccgcgccgt cgccctgagc aatctggaag tcgcctttgg agaggaattg
     2221 tcgaccttgc agcgccgcaa aattatgcgc gaatcgtatc agcagttcgc gcgcacgatg
     2281 agcgactttc tctggagccc gcgactgacg cggcaaaact tttcggtcta catcgagctg
     2341 gaaaatttcg aggaagctct ggaagccggt ggggccgagc gcagcatcat gatcgcctgc
     2401 tttcactaca gcaacttcga gtggctcagc ctcgcctgcg gatacctcgg tttgccaggc
     2461 acgatcatca tggagcggtt gaaaaactct ctgctcgatc cgattttcaa aaggctgcgg
     2521 gaacaatccg gccacgcgct gatcccccgc gagcgcggaa tcatccgtct ttacaaagtg
     2581 ctgcggagaa aaggccgcac cgcgttgctt gtcgatctta ccgtgccacc agcccaaggc
     2641 gccgtcgtga tcgaatgttt cgggctaaag acgtgcgtca cctccgcgca cgcgtggctg
     2701 catgaacgca cgggcgtatc gattatcccg gcgcactgtg agccggcgcc ggaggggcgt
     2761 taccgcttgg ttttccatcg taagatcgac aatgtgcaag gaatgacgca ccagcaaatc
     2821 gcgcaggcgt gctgggattc tttcgagcct tacgtgcgca aaaatcctgc gccgtggctt
     2881 tggatgtata aacattggcg ctacaaaccg gcggatgcgc gacggcgtta tccgttttat
     2941 gccaatgtcg cgccacgatt tgagaaactg ctgcaccgcg aacagcgcga tgccgatcat
     3001 tttgtcgacc ctcaccgcaa tcctctccct cagggagagg cggacgcgaa gcgcccatga
     3061 gggtgtgggc tcgggagcag gaacgtcgtt acagtaccgc agttgttgac aaaagttggc
     3121 cgagcttctc cagtatctgg ttatgcgagt tatctccccg ttgtttttca ttgccgcgct
     3181 tgtttgcgcg acgctgttca ccagttgcga gaccatgcgt ttggccggtg gcggcggagc
     3241 ctaccacgtg accgcctaca aaccgcacga tccttcggcg gtgcgcgtga aagtttcgct
     3301 ctccaaacag aacgtgtacg tgatggaagg cgaccgctgc ctcatgtcgg cggcttgctc
     3361 ggtgggttta cctaacaaac cgacgccgca cggtaatttc accatctact cgaaacagga
     3421 acacaagcgg tccggctcct atggtttcag tgtgcagggc aacaatgtcg tgccgaccac
     3481 ctccggccac acgtccggac gttacgttgg ctatccgatg gggtactggt gtgagtttgc
     3541 gccgggctat ggatttcatc agggcttcgt tcacccggtg ccgcggacac atggttgtat
     3601 tcggttgaaa ggcgaagccg cgccgaaatt tttcgcgctc gtgcggaccg gcacaccggt
     3661 gaatatcgcg acctcgcaac cggaagacgc gacggttggt ccgaaagtgc agcgggtgga
     3721 tgattcgcgc acacccgatc cggacccgca catcatggtt acggacgcgg cgtttcaaaa
     3781 acccgaagga caactattgg agtagccgag gcggcctgac gccgcaccaa cccgtgagca
     3841 caaatctcat ctccagcggc acgacggcgc gcgagaaggt gaacttgcgc acgcccgacg
     3901 tgatggcggc ggtgcaggag caggtcgagt cgcattatcg cagcgacatt gtcgagaaga
     3961 tccgccgcgc cggcggcatc atttcagttg gcgacacgac agtgcggttg gcaaagcagt
     4021 tcggcttttg ctatggggtc gagcgcgcaa tcgatctggc ttacgcggcg cgcaaagttt
     4081 tcaaggaccg ccggctcttc attgtcggcg agatcattca caacccggag gtgaaccaac
     4141 agattgcctc gttaggcatc aagaacctga ccgggcctaa caagcaagcc gatatctcgg
     4201 atctcgggcc ggaagatgtc gtcattgtgc cggcgttcgg cacggaattg tcgatccaac
     4261 aggaaatcaa agagcgcggc tgccaggtcg tggacacgac ttgcggtgac gtaatgagcg
     4321 tgtggaagcg cgtgcgcaaa tacgccagcg aatcggctac ctcgatcatt cacggtaaag
     4381 ccgagcacga ggaaacgaag gcgaccagct cgcgcgcgct cggcgacggc aatggtcatt
     4441 atctggtcgt gctcacgctc gcagataccg actacgtttg cgattacatc cggcacgggg
     4501 gcgataagca ggcgttcttc gacaaattta aaggcgccta ctcgccggga ttcgatccgg
     4561 acgtacacct gaaaacggtt ggcgttgcca atcaaacaac catgttgcgc ggcgagactg
     4621 aggaagtgca gcgccgcgtc cggcgggcga ttgtcgatcg tgacggtccc gagctcgcag
     4681 aaaagaattt ccggttcttc gacacgattt gcggcgcaac gcaggaacgg caggacgcgc
     4741 ttcgcgagtt gctcaatgtc ccgcttgatc tgctgctcgt ggtcggaggc tacaacagct
     4801 cgaacacgtc acatctcgcg gagatgggcg aggagaagtt gccgacctat ttcgtgctca
     4861 atgcctcgcg gctggtatcg gccaacgaga tcaagcatta caacctgcac gagaaacgcg
     4921 aagtcgtgtc gcgcttctgg ctgccgagcg gcccggcggt gatcggcatt acggccggtg
     4981 cgtcgtgccc gaacaatctc atcgaagaaa cgctgattcg tttgttcgag ctgcgcggta
     5041 tcccgcgcca gcaactcgag ctcgccgcgt aggtggatcg gcttctccga aggcgatgtt
     5101 aaggaagtgc ggcttcgccg cctagattag caacgcgctc ggagagcgcg ttccaccgaa
     5161 gatcgccatg ttcatccacg cgtttcctac gcgccgtgcg aataaccatt gtcatgcgag
     5221 caatttggaa aggcagcatc agtttcggcc tggtcaacat tccgatcgcc ctgtatcccg
     5281 cgacgcagaa ggaagagttg aagttcaggt tactgagaaa atccgatctc agcccaatca
     5341 actacaagcg cgtggcggaa aaggacggca gggaagtacc ttgggacgag atcgtcaaag
     5401 gttacgagta cgacaagggc aaatacgtcg tgctaaaaga cgaagatttc cagcgcgtcg
     5461 acattgaagc gacgcagacg gtcgacattc aggatttcgt caatgtcgac gagatcgacc
     5521 cgatgttctt ctacaagcct tattacctcg agccacaaaa gggcggcgat aaagcctatg
     5581 tgctgctgcg cgattcgttg aaggactcaa ataaggtcgg cgtcgcaaaa gtcgtcatca
     5641 aaacccggca gtatctcgcc ggggtaaagc cggaggatgg cgtactggtc ctcgagctga
     5701 tgcatttcgc cgaggagctg gccgatcccg agaagcttca cgtgccgaaa aagctcgacg
     5761 ccggcaaacg tgagatggac atggccaagg cgctgatcaa cagcatgact tcaaagtgga
     5821 acccggagaa gtaccacgat gactaccgcg aagcggtgat gaaagtgatc gaggagaaag
     5881 tcgaacacgg cggcgaagaa atcgaggaga aaccaaagaa agcgccgaag ccgacgaagg
     5941 taatcgatct cgtccaggta ttacagaaga gtctcgaaca agccggcggc gcgaagaaag
     6001 tatcaacaac gaaacgcgcc gcgaaacatc cgcagcggca caagaaagcc gcgtaaagta
     6061 gcgcaagcct ctggcttgta ttctagggga tcgcaagcgt gacgcctgcg ctactcttga
     6121 atttcttgac acgtaagatc gacatcgtgc agcctgaccg gccatgaaca ctctcaatcg
     6181 atttgcggac ctgatctaca gcgtcttccg gctaattgtt gggttgttat ttgcctgtca
     6241 cggcggccaa aagcttctcg gtttcccgcc aggcggcgat ggcgccggac aaggaatttt
     6301 cctccttggc gcgtggattg aactcgtggg cgggttgcta atagcttttg gttttctgac
     6361 tcgccttgca gcgttcatcg cagccggcga gatggcggtc gcgtacttta tggtccacgc
     6421 tgctggtaaa gctcttgatc acacaccgag tgccatcgaa caggtgtttc ccctgctaaa
     6481 taaaggtgag cccgctgtgc tttattgctg gtgttttctt ttttttcttt tctatggcgc
     6541 tggaggctgg agcatcgatg cgctgattcg caggggccgc gctgcaacac tgccagcggc
     6601 gactccgtaa cgtcgacttc cttgctgcta tgacacgcgg tttcgttgcg tttgcccttc
     6661 tgcttgccgc cgccgctggg gccgaagtga tcaaagactt tggccgcgaa cgtctgaaca
     6721 cttcgccgcg gcacgccgaa tgggtcgaca tcaaatcagg cacgcgcacg atcaaggcct
     6781 ttgtcgttta cccggagcgc aaagacaaag cgccggcggt gctcgtgatc agtgaaattt
     6841 ttggcctgac cgattgggtg cgcagtttgt gtgacgagtt ggccgagaac ggcgttatcg
     6901 cgttcgcgcc cgacttgcat agcggtcaga agtttgaaga tctcgacgcc gcgcgcaaag
     6961 tcacgtcagc gttaccgaag gagcaagtca aagccgatct cgacgccaca tccgattacg
     7021 cgctcacgaa aattccatcg tgcaacggga cgcttgccgt ttgcgggttc tgctggggcg
     7081 ggggagtaac attccaatac gcagcgacga atccgaaatt gaaagcggcc tattcgttct
     7141 atggcgtcgc cattgataac tccgatgacg cggccaaagt cgtctgtccc gtttacggct
     7201 tctacggcga gaacgacgag cgcgtgaacg tgaccattcc caaagcggaa gagctgatga
     7261 aaactgccgg caaaaaatac gagccggtca tctacaaagg cgccggacac ggtttcatgc
     7321 gctcaggcga gcctaacaac ccgggcgtgc gcgaagccga ccggaaggcg cgtgatgaag
     7381 cctggatgcg ctggaagaag ttactcgctg aacttgcggg ttccaggtca ggtggattga
     7441 gatctccgag ctcgatgtta acttgggcgg ccgaacg
//
LOCUS       DASTDG010000087         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1025336, whole genome shotgun sequence.
ACCESSION   DASTDG010000087 DASTDG010000000
VERSION     DASTDG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025336"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFO30_02700"
     CDS             complement(<1..879)
                     /locus_tag="VFO30_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET9418223.1"
                     /translation="MSAPIKPGAACAILAATLAFSLIFAANAFAQEMEAERVTVTGES
                     VIVTGSLIPTSELVGPNPISTLDRDLINKSGQGTTTEELLKSQTVASANSIPVQNNPD
                     RRGGPPGAASISLRGFDPGATLVLIDGRRVAPYPGTSPGGAAFFDLNTIPIAAVQSIE
                     IQKDGASTTFGADAVAGVVNLKLYKDYRGAQLTLYYGNTLDKDAGEYSSDILFGTGDD
                     KTSITGDIFFYHRNSMFNRDRGNSNRPPFLSSNSSPYNLQLSSDVVAAAGGENLNPGG
                     FEFATPPDFTNGLVPAS"
     gene            1138..1917
                     /locus_tag="VFO30_02705"
     CDS             1138..1917
                     /locus_tag="VFO30_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET9418224.1"
                     /translation="MNPDSSAPTIAHAAEIRRRKIFADIGYAFWKEEPSLRDAIFLFD
                     PEVIREVYVVPNFISEGYFTQTVVPRELELNGRTTKRADGQIWKYCEPVGNHALMTDL
                     LLRRAHEIAPGIAESDTSLLIVAHGTDLNENSAVAAKREVQKIRELGAYAEVQNVYME
                     EAPRVADWRRLTTTRNVVVVPFFISDGLHSYEDIPALLGIAQTRSTNASGEVFLSNPH
                     SIDNRFLFYGSSIGTDPRLADVILAQVEAFDREKLHTSAAS"
     gene            1945..2403
                     /locus_tag="VFO30_02710"
     CDS             1945..2403
                     /locus_tag="VFO30_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9418225.1"
                     /translation="MNPQTLKRLQEIGAFVLASGCKRKDMTKVAEMIRAARNYRWVGL
                     YKIVKDDFVIIAGTGTEPPAYRRFPKTQGLCGAALDSGKSMIVGDVRKDVRYLPTFHT
                     TRSEIIVPMRNDHHHIVGMLDAESDQVNAFGDDDRQFLERAGVLIAHCLK"
     gene            2419..3060
                     /locus_tag="VFO30_02715"
     CDS             2419..3060
                     /locus_tag="VFO30_02715"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HET9418226.1"
                     /translation="MLDPVEQALEREIGESCCIGEIFIRPTPVGGFALCHRDDESNDA
                     VQIFRGAEAAVEIAKYDDRGNYRPLKTAPTLRHGWRLELVDLAELRRALDYLYPGRLA
                     MLSAWKQHRLATPPLRETLDRQSGMYRVAAKISDGQIDEVVGNFCRSDGGCLRTILWR
                     RDGTGKIASTKLPPQKFDPSHDQTGRGQRAIPMLCQEACHLLVNECRKAVKAE"
     gene            3033..>4047
                     /locus_tag="VFO30_02720"
     CDS             3033..>4047
                     /locus_tag="VFO30_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9418227.1"
                     /translation="MSQSREGRMILRARVVVTMAGRPLENGAVAISGDQIVDVGRFKD
                     LRARNSGEIVDLGERALLPGLINAHCHLDYTCLRGKVPRQKSFADWIRAINAEKAKLA
                     PEDYVASINNGFTEVKRFGTTTAVNLTAFPQLVPQISSPIRTWWFGELINVRGTADAD
                     LAAKFLRSAEHWGLAPHAPFTASAGLYRRCERLASLLTTHIAESHEEMSMFRDASGPL
                     YDFLKELGRDMSDCGGKTPLACFLTLGDSSTPRGVPKEWMVVHLNELTDSDFNLLEKS
                     PRNFSIVHCPRSHSYFGHSPFQFGRLRSLGFNICLGTDSLASNDDLSLFAEMRAFQKE
                     FPNV"
BASE COUNT          875 a         1113 c         1207 g          852 t
ORIGIN      
        1 gcttgccggg actaatccgt tcgtaaaatc tggcggagtc gcaaattcaa accctcccgg
       61 atttaaattc tctccgccag cggcggccac cacatcgctg ctcaactgga gattgtacgg
      121 actggagttc gagctcaaaa aaggcgggcg attggagtta ccccggtcgc gattgaacat
      181 cgagttacgg tgatagaaaa agatgtcgcc ggtgatactt gttttgtcgt cgcccgtgcc
      241 gaaaagaatg tcgctgctat actcgcccgc gtctttgtcg agcgtgttgc cataatacag
      301 agtcaactga gcgccgcggt agtctttata gagcttgagg ttcaccacgc cagcaaccgc
      361 atccgctccg aaagtggtgg aagcgccgtc cttctgaatt tcgatgcttt ggaccgcggc
      421 aatagggata gtatttagat cgaagaaagc ggcgcccccg ggggaggtcc ccggatacgg
      481 ggcgacacgg cggccatcga tgagaacaag tgtggcgcct ggatcgaaac cacgcaggga
      541 gattgacgcc gcccccggcg ggccgccgcg ccggtcgggg ttattctgaa ccggaatgct
      601 gttcgcactc gccaccgtct gactcttgag aagctcttcc gtggtggtgc cttgaccaga
      661 cttgttgatc aagtcgcggt cgagggtgga tatcgggttg ggtccgacta actccgaggt
      721 tggaatgagg gagccagtca caatcacaga ctcgccagtc acggtcaccc gctcggcttc
      781 catctcctga gcaaatgcgt tcgctgcgaa aattaaagag aaggccagcg tcgccgcaag
      841 gatagcgcac gcggctcctg gcttaatcgg cgcgctcatg gttagacccg aaggtccacg
      901 caaattgcga ttgagccgat ttccatgaaa acttccaatc taaatcacgg gcaacggcat
      961 cgggcggttt cctaaacgag gcaggagtaa cgaaaaggcg acccggacca atggcttatc
     1021 cttgtctctg cgattcggcg ttggacccat tcaacttgcg ctcaaggcag gctgttgaac
     1081 gtttcgtctg tgagatccgc gaacagcgcg cttttaatcg tcgcgcacgg ctcgaccgtg
     1141 aatccagatt caagcgcacc gacgattgcg catgcggcgg agattcggcg acggaaaatc
     1201 tttgcagata tcggctacgc gttctggaaa gaggaaccca gcttgcgcga cgcaatcttc
     1261 ctgttcgatc cggaagtgat tcgcgaagtc tatgttgttc cgaatttcat cagcgaaggt
     1321 tatttcacgc aaacggtcgt tccgagagaa cttgagctga atggccggac gaccaaacgc
     1381 gcagacggtc agatctggaa atattgtgaa ccggtaggga atcatgcttt gatgactgac
     1441 ctgttgctgc gacgggcaca tgaaattgca ccaggcattg cggaaagcga cacgagtctc
     1501 ctgatcgtgg cgcacgggac tgacctgaac gaaaacagcg cggtggcggc aaaacgcgag
     1561 gtacaaaaga tccgcgagct cggcgcgtac gcggaggtgc aaaacgtcta catggaagag
     1621 gcgccgcgag tggcggattg gcggcggtta acaaccacac gcaacgttgt tgtcgttcca
     1681 tttttcatct ccgatggatt gcacagttac gaagacattc cagcgttgct cggaatcgcg
     1741 cagacaagat cgacaaacgc gagtggtgag gttttcctca gcaatccgca cagcatcgac
     1801 aatcgctttt tgttttacgg gagttcaatc ggaaccgatc cccgtctggc cgatgtgatt
     1861 ctggcgcagg tcgaggcgtt cgatcgcgaa aagttgcaca cgagcgcagc ttcgtgacat
     1921 ttgcgcacgc cgtcgcttcc aaggatgaac ccgcagacgc ttaaacgctt acaggagatc
     1981 ggagcgtttg tcctcgcgag cggctgcaaa cgcaaggaca tgacaaaagt cgcggaaatg
     2041 atccgcgccg cgcgcaatta ccgctgggtc ggcctttata aaatcgtgaa ggacgatttc
     2101 gtgatcatcg ccggcacggg cacggagccg ccggcgtatc ggcgttttcc gaagacgcag
     2161 ggactctgcg gcgcggcttt ggattcaggc aagtcgatga tcgtcggcga tgtgcgcaaa
     2221 gacgtgcgct atctgccgac gttccacacc acgcgttcgg agatcatcgt cccgatgcgc
     2281 aacgatcatc atcacatcgt tggcatgctc gacgccgaaa gcgaccaggt gaatgccttc
     2341 ggcgatgacg accgccaatt cctcgagcgc gccggcgtgc tgatcgcgca ttgtctgaag
     2401 taaccgccga acggcgcgat gctcgatccg gttgagcagg ctctcgagcg cgagattggt
     2461 gagagttgtt gcatcgggga gatttttatc cgcccaacgc ccgtcggcgg atttgccctc
     2521 tgccaccgtg acgatgagtc aaacgatgcg gtgcaaattt ttcgcggcgc tgaagcggcc
     2581 gtggagatcg cgaaatacga cgataggggc aactatcggc ctctgaagac cgcgccgact
     2641 ctgcggcacg gctggcgatt ggaacttgtc gatcttgccg agctgcggcg cgctctggat
     2701 tacctctatc ccggccggct cgcgatgctg tcggcttgga agcaacaccg cctcgcgacg
     2761 ccgccgctgc gtgaaacgtt agatcgacaa tcgggtatgt atcgcgtcgc agcgaaaatc
     2821 tcagacggac aaatcgacga agtcgtcggg aatttctgcc gctcggatgg cggttgcttg
     2881 cgaacaattc tctggcgacg cgatggaact ggcaagatcg cgtcgacaaa gctcccgccg
     2941 cagaagttcg atccgtcgca tgaccagacg ggacgcgggc aacgcgcgat tccgatgctc
     3001 tgccaggagg cttgtcatct gctcgtgaac gaatgtcgca aagccgtgaa ggccgaatga
     3061 tcctgcgcgc gcgcgtcgtc gtgacaatgg ccgggcggcc gctcgagaac ggcgcggtcg
     3121 cgatttcagg cgaccaaatt gtcgacgttg gcaggttcaa ggatctccgc gcacgcaact
     3181 ccggcgagat tgtcgatctt ggcgagcgcg cattattgcc gggcttgatc aacgcgcatt
     3241 gtcacctcga ttacacttgt ctgcgcggga aagttccgcg gcagaaatcg ttcgccgatt
     3301 ggattcgcgc gatcaacgcg gagaaggcga agctcgcgcc ggaagattac gtcgcttcga
     3361 tcaataacgg tttcacggaa gtgaaacgtt tcgggaccac gaccgccgtc aatctgaccg
     3421 cctttccgca gctggtcccg cagatttcct ccccgattcg gacttggtgg tttggcgaac
     3481 tgatcaacgt ccgcggcacc gcagatgccg atctggcggc gaaatttcta cgatcagcgg
     3541 agcactgggg gctggcgcca cacgcaccat tcaccgcttc ggcagggttg taccgacggt
     3601 gtgaacgact ggcgtcattg ctgacgacgc atatcgccga gtcacacgaa gaaatgtcga
     3661 tgttccgcga cgcgagcggg ccgctctacg atttcctgaa ggagctcggt cgcgacatga
     3721 gtgattgcgg cggcaagacg ccgctcgctt gttttttaac cttgggagat tcgtcgactc
     3781 cgcgcggagt gccgaaggag tggatggttg ttcatctgaa tgagctcacc gacagcgatt
     3841 ttaatttgtt ggaaaaatca cccaggaatt tttcgattgt gcattgtccg cgtagccatt
     3901 cctactttgg ccactcgccg tttcagttcg ggcggttgcg ctcgctcgga ttcaacatct
     3961 gcctcggaac cgacagtctc gcgagtaatg acgatctcag tctgtttgcg gagatgcggg
     4021 cgtttcaaaa agaatttcca aacgttt
//
LOCUS       DASTDG010000088         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1010012, whole genome shotgun sequence.
ACCESSION   DASTDG010000088 DASTDG010000000
VERSION     DASTDG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010012"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFO30_02725"
     CDS             complement(<1..1135)
                     /locus_tag="VFO30_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418228.1"
                     /translation="MNVLEPITSTLAEESRPILWRALERARIVGNFALIQGVVQLIGF
                     VSGILIVRTLAQRDYAYFTIANTMQGTISVLADIGISVALISIGGRVWQDRHRFGQLV
                     NTALALRKYLGAVVVLVNAPVLWFLLFRNGASALYTLVLVLLVAATVGVQLSNDVLSV
                     VPRLRSDIVRIQKIDFVGASVRLLALLALIYIYLNAGVAIAVATGVYLLQFAMLRNYA
                     RTVIDVDAPENDDDRKAMLGFVRKLAGNAIFFCLQAQIAVFLISFFGRRASSVAEVGA
                     LSRLAMIFALLSNLLTNVFVPAFARAQSPRRLRWLYAGIVGGVSAFGLSILLGANLFP
                     NEFLLVLGGKYAHLHAELVLMIGSALLSSITATLWALNAARAWV"
     gene            complement(1237..3387)
                     /locus_tag="VFO30_02730"
     CDS             complement(1237..3387)
                     /locus_tag="VFO30_02730"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET9418229.1"
                     /translation="MQAAIADKSTPAAWSTALITRLHRYRALLVRRWWIPVFTVCLGI
                     FVEAWLIYQTPPSFLSTSKMMLAGKLNIAQGAVYSEDSVNFYGTQIQLMQSAEVKRSA
                     EALVRTTHPELQLVPVEINVAQKPRTSIFDLQAIGRSPDYTQAYLNAVMQKYLDFKKG
                     MREGQGQTVISGITEQLVQTEKDLRGAEDEMLEFQKQNNIGFIQEEGNSAAAYLVKLN
                     REYASLKTEYDLLNLLDLDQNLDRAQIRPEAAASPQPNEEQGLPFAGVGPEADYLKAK
                     QQLQMLKAERDTLSKDLRPKHPKIIKLDDEISKQEKLIDLFRADTVEKLKTRRESIGK
                     QMQNLQANIKEWEAKALDLSQRLAQFNRVKGKVDRLKTLYDRLTNNLKEVDVSQVVDS
                     GDQVSIMEMATAPVSVRPGLVKSLLIGLGCGLLAGIAILVLLDRIDDRMASFNEFQHE
                     FSENVLGQIPKDKTKGEVTLLQADDARHVFAESYRNVRSSIFFMPYEGPRPKTLLITS
                     AVPNEGKSTVAANLAITMGLSGASTLLIDADLRRGGLREAFGVSSKIGFSEVLQQEVN
                     WKEVVVPTLYPNLFLLPRGKTATQPSEYLLRESTDAILRELYNNYDYIIIDSSPVLAA
                     DDTTSLAPKIDATLFVVRLSYTSARLTRKALEQLYSRQVNVPGVILNFVDTSLPEYYY
                     YQYSEYYSVPAAGGDGEAAAPTPPREEVKPVQFS"
     gene            complement(3389..4078)
                     /locus_tag="VFO30_02735"
     CDS             complement(3389..4078)
                     /locus_tag="VFO30_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9418230.1"
                     /translation="MRLRLYVLSFTAIVLVNPPGRAQDSYSPVPTIAPLAPVTESAPP
                     ATNAVMHTNSMDVLDDKKRLGRNDYVSFRVVEDRDNDSQRLRVNDSGDLEVPYVGLVP
                     AEGKTCKELAYRIKGALEKEYYYHATVILAVDRISEKSRGRVYIYGSVKAQGPEELPP
                     NEVFTVSKAVIRAGGFGDFANKRKVKVTRKNGRDFTVDLKRVIEEGHTEEDLVLEPDD
                     QIYVPQRLINM"
     gene            complement(4100..5398)
                     /locus_tag="VFO30_02740"
     CDS             complement(4100..5398)
                     /locus_tag="VFO30_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9418231.1"
                     /translation="MSDHSPSCVRARRSKKFFAGTLLVGVLCAPSILRAQDAVRPSLA
                     GEAAAEARRQDVDRIPYNLMMGPVRFRLSATVGVEYNDNINFAEKDTEDDVIVRPNID
                     IDAIWPLTQLNTLRLDLGLGYAFYLDHSENDTNGILIAPKSQIALDIFVGDFRINVHD
                     RMQLQQDPIQQPALSNVTDYGRFENTVGVGALWDLNKALLTLGYDHYTYVSTTSDFDY
                     LNRNAEELSAAIQVLVSSTTGVGIEGNAVFTYYDQHVLNDSDTYSVGAFVESQLTNNL
                     KVRAAGGYQRINFDRNAVDFFGVLAPDQKDLDDFYANILIGHRLNVVFSHSLSAGHES
                     QLGINSNYITLNYVRYTTTWNLIRRTLLSTEFFFEDADESGGFIDEHFQRVGGAITLG
                     YQLTPHVTLGLRYQGTSKDSDVALRDYTQNRVSLDGTYSF"
     gene            5651..6265
                     /locus_tag="VFO30_02745"
     CDS             5651..6265
                     /locus_tag="VFO30_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418232.1"
                     /translation="MNASRFFSGSRFCRDAFLGLFVLASCVLLSSVSLRAYADDASSI
                     SDKIECITPDGRVTAISHFNAASTDIPASVHARDTISCELQEGQTIFIIPFPKGALVD
                     RFTFINQNAAACGELNIAVSECHLAADSPRWVGVEGIVPFAHKRLFNLSMVGINARYV
                     KLSFRVENTSAVSRADHHSKQQFVAPPTIAGAEAAPPATAPAEP"
     gene            6420..8069
                     /locus_tag="VFO30_02750"
     CDS             6420..8069
                     /locus_tag="VFO30_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9418233.1"
                     /translation="MHLATRDDQIKPVAASSEHHEAEPAYDKQTNDRRVLEDHSSQSG
                     DHLQILARATNDAVRDWDVKTGRLCWPQGLDTLLGYCRSSVVEEIGFWHQSVHPEDLR
                     RIVPRIADALNGNSERWSDEYRFRRADGNYIVLLERALIIRDASGRAVRFVASLMDVT
                     ARKQLQDQICRSQRMEAFGQLAAGVAHDFNNFLTTILGYSDLILAEANRKSAVADHIK
                     EIRGAAGRASALTSQLLAFSRKQALESRVVDINSLITNLERSLLRLLGDNIHIVCHLH
                     DESDAAHIKVDPGQFNQVIVNVAVNARDAMPQGGQLTIRTSRLDVAPQSEHASVYSEF
                     KPGEYVLVSVSDTGEGMTDEVKAHLFEPFFTTKDTGSGLGLATAYGIVRQSGGQIQVQ
                     SEPGKGTTVQIYFPRVPALPPPPYKKPSAAKLPTGTETILVLEDDISVRHVSIRILRS
                     LGYNVIEAANGDDAQRLISQSAGEHIDLLLTDVVMPQMSGRFFADWLRKLRPRTKVIF
                     VSGYLSESLNAGDKIDRGTYFLPKPFDPEQLAQTIRRVLDG"
     gene            8172..>8456
                     /locus_tag="VFO30_02755"
     CDS             8172..>8456
                     /locus_tag="VFO30_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418234.1"
                     /translation="MQSREHEIDTRPSGSDFAQRHIGPSEPEVAEMLRDIGFSDLDAL
                     IDATIPKNIRLDRALNLPEGKSEAEALAELRAISKQNKLAKSFIGAGYSGC"
BASE COUNT         1819 a         2416 c         2390 g         1831 t
ORIGIN      
        1 cgacccaggc gcgcgcggca ttcagcgccc aaagcgtggc ggtaatggag gagagaagcg
       61 cgctgccgat catgagcacg agctccgcgt ggagatgtgc gtacttgccg ccgagcacaa
      121 ggagaaattc gttcggaaag agatttgccc ctaacaagat cgacaaccca aatgcgctga
      181 cgccgccgac gatccccgca tacaaccagc gcaatcttcg cgggctttgg gcgcgagcaa
      241 atgccggcac gaagacgttc gtcagcagat tgctaagcaa agcgaagatc atcgcgagcc
      301 gacttaatgc gccaacttcc gcgaccgagc tcgcgcgcct accgaagaag ctgataagga
      361 aaacggcgat ttgggcctgc agacaaaaga aaatcgcgtt gccggcaagt ttgcgcacga
      421 acccgagcat ggccttgcga tcatcgtcgt tctccggcgc gtcgacatcg atcacggttc
      481 tcgcataatt acggagcatc gcgaattgca gcaggtaaac tcccgtcgcg acggcgatcg
      541 ccactccggc gttcaggtaa atatatatca gcgcgagcag cgcgagcaga cggacgctcg
      601 caccgacgaa atcgatcttc tgaatgcgca cgatatcgga gcgcagccgg ggaaccacgc
      661 tcaagacatc gttcgacaat tgcacgccga cagtcgcggc aaccaacaga acgagaacca
      721 gcgtgtagag cgcagaggcg ccgttcctga acagcaaaaa ccacagcacc ggcgcattaa
      781 cgagaacgac aactgcgccg agatatttcc gcagcgcaag cgcagtattg acaagctgac
      841 cgaagcgatg tcgatcttgc catacgcggc cgccgatcga aatgagggcg acgctgatac
      901 cgatatcggc cagcacgctg atcgtgccct gcatcgtgtt cgcgatggta aaatacgcgt
      961 aatcgcgctg cgccagagtt cggacaatga ggatgccgct gacgaagccg ataagttgaa
     1021 cgacgccttg gataagggcg aaattgccaa cgatcctggc gcgctccaat gcgcgccaga
     1081 gaatggggcg cgattcttca gcgagcgtag atgtgatcgg ctcgagaaca ttcatcacgc
     1141 ggaatcgttt gcgctacgga gaagatgtcg atctaacact ttcgccggcg ggacgcccga
     1201 aaagtgcaga tcacgaaggc aaaatcgccg gtcagcttac gaaaactgaa ccggtttcac
     1261 ttcttcgcga ggtggtgtcg gtgccgccgc ttcgccatcg ccaccagcag ccggcacgct
     1321 gtaatactcc gagtactggt agtaatagta ctccggcaag gaggtatcga cgaagttgag
     1381 gatgacgccg gggacgttga cctggcggct gtaaagttgc tcgagcgcct ttctggtcag
     1441 gcgcgccgag gtgtaggaga ggcggacgac gaagagagtg gcgtcgatct tgggcgcgag
     1501 actggtggta tcatcagcgg cgagaacggg cgagctgtcg atgatgatgt agtcgtaatt
     1561 gttataaagc tcgcgcagga tggcgtcggt cgattcgcgc aaaagatact cgctcggttg
     1621 tgtggccgtc ttgccgcgcg gcaacaggaa cagattcgga tacagagtcg ggacgacgac
     1681 ttccttccag ttcacttcct gctgcaatac ttcggagaaa ccgattttcg acgagacacc
     1741 aaaagcttcg cgcagcccgc cacgacggag atcagcatcg atcagcagtg tggacgcgcc
     1801 ggagagcccc atagtgatgg cgagattcgc ggccacggtg gattttcctt cattcggaac
     1861 ggcgctggtg atgagcagtg tcttgggccg tggaccttca tagggcatga aaaaaatcga
     1921 cgagcgaacg ttccgatacg actcggcgaa aacgtggcgg gcatcgtcgg cctgcaagag
     1981 cgtgacctcg cccttggttt tgtcctttgg aatctggccg agcacatttt cagagaactc
     2041 atgctggaat tcgttgaagg aggccatgcg atcgtcgatg cgatcgagca gaaccagaat
     2101 cgcgatgcca gcgagcaatc cgcaaccgag gccaatgagc aggctcttga ccaagccggg
     2161 ccgcacggag acgggggccg tcgccatttc catgattgag acctggtcgc cggagtcgac
     2221 gacctgagag acgtcaactt ctttgaggtt gttcgttagg cggtcgtaaa gcgtcttgag
     2281 ccggtcgacc tttcctttga cgcgattgaa ctgcgcgagt cgctgactga gatcgagtgc
     2341 cttggcttcc cattccttga tgttcgcctg gaggttctgc atttgtttgc cgatcgattc
     2401 acggcgcgtc ttgagcttct cgacggtgtc cgcgcggaac aggtcgatca gtttttcctg
     2461 cttcgagatt tcgtcgtcca gcttgatgat cttcggatgt ttcggtcgca gatctttgga
     2521 gagggtgtcg cgctcggctt ttagcatctg cagttgctgc ttggctttca ggtaatcggc
     2581 ctccgggcca acgccggcga agggcagtcc ctgctcctcg ttcggttgtg gactggcggc
     2641 tgcttccggc ctgatctgcg cgcgatcgag attttgatcc aggtcgagaa gattcagcag
     2701 gtcgtactcg gttttgagac tggcgtactc acgattgagt ttaaccaggt aagcggcggc
     2761 gctgtttcct tcttcctgga taaagccgat gttgttctgc ttctggaact cgagcatctc
     2821 gtcttcggcg ccgcgcaagt ctttttcggt ttgcacgagc tgctcagtga tgccggaaat
     2881 caccgtctgg ccctgccctt cccgcatccc ttttttaaag tcgagatact tttgcatgac
     2941 ggcattgaga taagcctgcg tgtagtccgg cgatcgcccg atcgcctgca gatcgaagat
     3001 agaggtgcgc ggtttttgtg cgacgttaat ctccaccggg acgagctgca gttccggatg
     3061 cgtggtgcgg acaagcgctt cggcgctgcg tttcacctct gcgctttgca tcagctggat
     3121 ctgtgtgccg taaaaattca cgctgtcttc gctgtaaact gcgccttgcg cgatgtttaa
     3181 cttgcccgca agcatcatct tgctcgtgga gagaaaggag ggcggcgttt gatagataag
     3241 ccaggcctcg acaaaaatgc cgagacagac ggtgaacacg ggaatccacc aacgcctgac
     3301 caacaacgct ctatagcgat gcagccgcgt gatgagcgcg gtcgaccatg cggcaggagt
     3361 ggatttgtca gcgatggcag cttgcatctt acatgttgat caagcgctgc ggcacgtaaa
     3421 tctgatcgtc cggctccagc accaggtctt cttcggtgtg gccctcctcg atgacgcgtt
     3481 taagatcgac agtgaaatcg cggccgttct tgcgcgtgac tttcaccttg cgcttgttcg
     3541 cgaaatcgcc aaaaccaccg gcgcggatta cagctttgct gacggtgaaa acttcgttcg
     3601 gcggcagctc ctccgggcct tgtgccttga ccgagccgta gatgtaaacc cggccgcgcg
     3661 atttttcgct gatccggtcc accgcaagga tgacggttgc atggtaataa tattcttttt
     3721 ctagcgcgcc cttaatgcga taggccaact ccttgcaggt tttcccctcg gccggcacca
     3781 gaccgacgta tggcacctcg agatcgccgc tatcatttac gcgcagtcgc tgtgagtcgt
     3841 tatcgcgatc ttcgacgacg cgaaaactga cgtaatcatt gcggccgagc ctcttcttgt
     3901 cgtccagcac atccatcgag ttggtgtgca tgacggcgtt cgttgccggt ggcgccgatt
     3961 ccgtgaccgg cgcgagtggc gcgatcgtcg gaactggact gtaggaatcc tgcgcgcgtc
     4021 ctggggggtt gaccaaaacg atggcagtga aacttaagac atacaggcgc aatcgcatta
     4081 acattcccgt ttcattccct tagaaactgt aagtgccatc aagtgaaacg cggttctgcg
     4141 tataatcgcg cagtgccacg tccgagtctt tgctcgtgcc ctgataacgc agacccagag
     4201 tgacatgcgg cgtgagctgg taaccgaggg tgatcgcgcc gccgacccgt tggaaatgct
     4261 cgtcaatgaa accgcctgac tcatccgcat cctcaaaaaa gaactcagtg gagagaaggg
     4321 tgcggcggat gaggttccag gtggtggtgt agcgcacgta attcaatgtg atgtagtttg
     4381 aattgatgcc gagctggctt tcatggccgg cgctcaagct gtgcgaaaac accacgttca
     4441 gccgatgacc gataaggatg ttggcgtaaa aatcgtctag atctttttga tccggcgcca
     4501 gcacgccgaa gaaatcgacc gcgttgcgat cgaaattgat ccgctggtaa ccgccggcgg
     4561 cgcgcacctt aagattatta gtcagttggc tctcgacgaa tgcgcccacg ctgtaagtgt
     4621 cggagtcgtt cagcacgtgc tggtcgtaat acgtaaagac ggcattgccc tcaatcccga
     4681 cgcccgtggt ggaactgacc aagacttgta ttgcagccga gagctcctcg gcgtttcggt
     4741 tcaaatagtc gaagtcactg gtcgtcgaca cataggtgta atggtcgtag ccgagtgtca
     4801 gcaatgcttt gttcagatcc cacaacgcac cgaccccgac cgtattttca aaccgcccat
     4861 agtccgtcac gttgctcaag gccggctgct gtatcggatc ctgctgcagc tgcatgcgat
     4921 catgaacatt gatgcggaaa tcgccgacga aaatatccag cgcaatctgc gacttgggcg
     4981 cgatcaaaat tccgttcgtg tcgttttcgg aatggtcgag ataaaaggcg tagcccagcc
     5041 ccaggtccaa acgaagcgtg ttgagttggg ttagcggcca aatcgcgtcg atatcgatgt
     5101 ttgggcgaac aatcacgtca tcctcggtgt ccttctcggc aaaattgatg ttgtcgttgt
     5161 actcgactcc gaccgtcgcg ctcaggcgaa agcgcaccgg gcccatcatc aagttgtacg
     5221 ggatgcggtc gacgtcctgg cggcgcgctt cggccgcagc ctcacccgct aacgatggac
     5281 gaactgcgtc ttgggcgcgg agaatcgatg gtgcgcaaag cacgccaacc agaagcgtcc
     5341 cggcgaaaaa ttttttgctg cgacgcgcgc gtacgcaaga aggagagtga tcgctcatga
     5401 ggattgaggc ccgttaggtt agcagttgtg gtaccatggc gtttgtcgtt gaaccggctc
     5461 tccggcggcg cgccgccgat ctgacgccgt tggcccttcg gcaaaatcgc tgatcccgtt
     5521 ttctcttttt aagtgggcga cttcttacac caaaattctt cgtccgagca agtgaaatca
     5581 gcgaaatctc ttcccgagcg actggcagcc gcgcctacac tggcccgatt taagctccag
     5641 gctgggcctg atgaatgcgt cacgtttttt cagcggatct cgcttctgcc gcgacgcgtt
     5701 cctgggccta tttgttctcg ccagctgtgt tctgcttagt tcagtttcac ttcgtgctta
     5761 cgccgatgac gcgagctcga ttagtgacaa aattgagtgc atcactcccg atggccgcgt
     5821 gaccgcgatt tcgcacttca acgccgcatc aactgacatt ccggcatcgg tccacgcgcg
     5881 cgacacgatc agctgcgagc tccaagaagg ccaaaccatt ttcatcatcc cttttccgaa
     5941 aggcgcgctc gtggaccggt tcacttttat taatcaaaac gctgccgcat gtggtgaatt
     6001 gaacatcgcc gtatcggaat gccatctcgc cgccgacagc ccgcggtggg tcggcgttga
     6061 aggaattgtc ccattcgcgc acaagcgcct gttcaatttg tcgatggtcg gaattaatgc
     6121 gagatatgtg aagctgtcct ttcgcgtcga gaacacttcc gccgtaagcc gcgctgatca
     6181 tcattccaag cagcaattcg tcgctccacc cactatcgcc ggggccgaag ctgcgccgcc
     6241 tgcgactgcg ccagccgaac cgtaatttcg ccgcgctacg ccgaagtttt gatgtgtgac
     6301 ctcatacacg agccggatcg cttgctctcg tgcaatcact tgtcacctga cctagcgccg
     6361 attttgtgca caggcataga actagctcct tctacgccgg ctcttcactg tcgtgacgca
     6421 tgcatttggc tacgagggac gatcagatca aaccagtagc agcatcatcc gaacaccatg
     6481 aagccgaacc cgcctatgac aagcaaacga acgatcgccg agtactggaa gatcattcgt
     6541 cgcaaagcgg cgatcatctt cagatccttg cccgggctac gaacgatgca gttcgggatt
     6601 gggatgtaaa gaccggccgc ctctgctggc cgcagggtct cgataccctg ctcggttatt
     6661 gtcgatcttc cgttgtcgaa gagatcggtt tctggcatca aagcgtccat ccggaagatc
     6721 tccgtcgcat tgtcccacgc attgctgatg cgctaaacgg caatagcgag cgttggagcg
     6781 acgaataccg gttccgtcgc gccgacggaa actatatcgt cttgctcgag cgcgcgctca
     6841 tcatccgcga cgcttccggt cgcgccgtgc gattcgtcgc ttccctcatg gacgtgacgg
     6901 cgcggaaaca attgcaagac cagatttgcc gctcccagcg gatggaagcg ttcggccagc
     6961 tcgccgccgg cgtcgctcac gatttcaata atttcctgac caccattctc ggttacagcg
     7021 atctcatcct cgcggaggcc aatcgcaaaa gcgctgtcgc cgatcacatc aaagaaattc
     7081 ggggcgccgc cggccgcgct tctgcgttga ccagtcagct cctcgccttt agtcgcaaac
     7141 aagcgctcga atcgcgcgtc gttgacatca attcgcttat caccaatctc gagcgctcgc
     7201 tgctgcgact gctcggcgat aacattcaca tcgtctgcca tctgcacgat gaaagcgacg
     7261 cggcccacat caaggtcgat cccggccaat ttaatcaagt catcgtgaac gtggcggtga
     7321 acgcgcgtga cgcgatgccg cagggcggcc aattgaccat caggaccagt cgcctggatg
     7381 ttgctccgca aagtgaacac gcttctgtct acagcgaatt caaacccggt gaatacgtgc
     7441 tggtcagcgt cagcgatact ggcgaaggca tgaccgacga agtgaaggcg cacttgttcg
     7501 agccattttt cacgaccaaa gatactggca gcggtcttgg tctcgccacc gcctacggca
     7561 tcgttcggca aagtggcggc cagattcagg ttcagagcga accgggaaag ggcacaaccg
     7621 tgcagattta cttcccgcgc gttccggcgc tgcctccacc gccctataaa aaaccgagcg
     7681 ccgcgaaact gcccaccggc accgaaacga ttctcgtgct ggaagacgac atcagcgtcc
     7741 ggcacgtttc cattcgcatc ctgcgcagcc ttggctacaa cgtgattgaa gccgctaacg
     7801 gcgacgatgc ccagaggtta attagtcaga gcgccggcga gcacatcgat cttttgctta
     7861 ccgatgtcgt catgccgcaa atgagtggcc gtttctttgc ggattggctc cgcaaactgc
     7921 gaccgcgcac caaagtcatt ttcgtctccg gttatctttc cgaatcgctg aatgccggcg
     7981 ataagatcga tcgcggcacc tactttttgc cgaagccctt cgatcccgaa cagctcgctc
     8041 aaaccatccg ccgcgttctc gacggatagc gtgtcctgct ggtgctggcg atcatgctcg
     8101 tgcgcgatta gcaattgtag ccggcacgcg gaaacttgaa ccgcgattcg cgtctcgtag
     8161 tctgtccggc tatgcagtcg cgcgaacacg agatcgacac tcgcccgtcc ggctcggact
     8221 tcgcgcaacg ccacatcggt ccaagcgaac ccgaagtcgc agaaatgctg cgcgatatcg
     8281 gcttcagcga tctcgacgca ctgatcgacg cgacgattcc gaaaaatata cgcttagatc
     8341 gagcactgaa tctgccggaa gggaaatcgg aggccgaagc gctcgccgaa ctgcgcgcga
     8401 tttccaagca aaacaagctc gcgaaatcgt tcatcggcgc cggctattcc ggttgc
//
LOCUS       DASTDG010000089         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_727631, whole genome shotgun sequence.
ACCESSION   DASTDG010000089 DASTDG010000000
VERSION     DASTDG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727631"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFO30_02760"
     CDS             complement(<1..414)
                     /locus_tag="VFO30_02760"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9418235.1"
                     /translation="MAYELPKLPYPYDALEPHIDAQTMEIHHTKHHQTYITNVNNAIK
                     GKPDLEKKSVEDLIRDLNSVPEDIRTVVRNNGGGHANHSFFWKIMGPNAGGEAKGKLA
                     DDIKATFGSFNDFKEKFAAAGGARFGSGWVWLIRNK"
     gene            complement(616..2610)
                     /gene="shc"
                     /locus_tag="VFO30_02765"
     CDS             complement(616..2610)
                     /gene="shc"
                     /locus_tag="VFO30_02765"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET9418236.1"
                     /translation="MSRSTDVIALPSAGPDAAHAYSATDAEIWQAMHRAQENLLRQQR
                     PDGHWCGELIVDSTLCSDFVLFMHWFGEVDEQLQQRCVRHILKRQLPDGGWNIYFAGP
                     SEINASVKAYFALKLAGHSPDAPFMQEARANILRLGGVPRLNTFSKFYLALLGQFPWK
                     YLPAIPVEMILLPSWAPFNIYQMSSWSRAMLVPLAIINHFKPVRELPGLKQLHELYPL
                     GTEHKDFTLRRDARFFTWRNFFLRSDRVLKFVDRLPVHPLRRVALEEAERWMIERIGE
                     GSDGLAAVYPAMLNSLIALRALGYPKTHPIYEKARKDFAALFVDDQEDFRIQPCLSPV
                     WDTAINMIALTESGVSAESPTLQRAAKWLVDKEVGIRGDWAINNPHPEASGWAFEYNN
                     IYYPDTDDTAMVLMALRLVRPQDRQSIEQLFARALGWLLSFQCRDGGWAAFDKDVTKH
                     WLEDMPFADHNAILDPTCSDLTARMLELLGYIGFDPDEQAVRDAIQYLIDTQEDDGSW
                     YGRWGVNYIYGTWQVLRGLRAIGQDMTQDWILRGRDWLESCQNEDGGWGETCASYETR
                     ELKGIGESTASQTAWAVMGICACGDLDRPSIQRGLRFLLSRQQPDGSWEEPQITGTGF
                     PRVFYLKYDMYRQNFPLLALATYVNYRSGRGDRPSFYRCE"
     gene            complement(2650..>3193)
                     /locus_tag="VFO30_02770"
     CDS             complement(2650..>3193)
                     /locus_tag="VFO30_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HET9418237.1"
                     /translation="AAKKDMIKRLSLRPENFFLTRDMTVQKFQKIDEWMNRYTFFGTP
                     IYFEFLAGKRDLTCSAWAIPTRNIRGWKGPCYLMTDAHYSSYTELLEKVQWDRYGVVD
                     GVARDSRCENCMVHCGYEPTATLGLQAQRGDTWKNIKFNFGPRPKAKGNGSEVVVFNG
                     ASCGNGHLTGKNASASAQAS"
BASE COUNT          657 a          939 c          919 g          678 t
ORIGIN      
        1 cttattgcgg atcagccaaa cccagccgct gccgaagcgc gcaccgcccg ctgctgcgaa
       61 cttctctttg aaatcgttga agctgccgaa ggtcgccttg atgtcgtcgg cgagttttcc
      121 ttttgcctcg ccgcccgcat tcgggcccat gattttccag aagaaggaat ggttggcgtg
      181 gccgccgccg ttgttgcgga cgacggtgcg aatgtcttcc ggaacggagt tgaggtcgcg
      241 gatcaaatcc tcgactgatt ttttctcgag gtcgggtttg cctttgatcg cgttgttgac
      301 gttggtgatg tacgtctggt gatgcttggt gtgatggatc tccatcgttt gcgcgtcgat
      361 gtgcggctcc aaagcatcgt acggataagg taatttcggt aattcgtaag ccatacccag
      421 atgctaggcc gcgaagtttc gcggtcaatc gacgaccaaa gaacttttcg cacataaatg
      481 gcaaactcga cggcttaaag ccgtcgagcc taacaaatat cgcgccggct cggccgggac
      541 cgcgatgggc gaagtcgatc ttacacgtgg catcggcatc ctgccgatga gacatgggct
      601 ggaagcccat gcaacttact cgcagcgata gaagctgggg cgatcgccgc gaccgctgcg
      661 gtagttcaca taagtggcaa gcgcaagcag cgggaaattc tgccggtaca tgtcgtactt
      721 gagatagaag acgcgcggga aaccggtgcc ggtaatttgc ggttcctccc acgaaccgtc
      781 gggttgctga cggctgagga ggaagcgcag acctcgttga atgcttggcc ggtcgagatc
      841 gccgcaggcg caaatcccca ttaccgccca ggccgtctgg gaagcggtgc tttcgcctat
      901 gcctttgagc tcgcgcgttt cgtaagacgc gcacgtttcc ccccaaccgc cgtcttcatt
      961 ctggcagctc tccaaccaat cgcggccgcg cagaatccag tcctgcgtca tgtcctgccc
     1021 aatcgcgcgc aatccgcgca agacctgcca cgtgccgtaa atataattca caccccagcg
     1081 gccataccac gagccgtcat cctcctgcgt gtcgatcaag tattgaatgg cgtcgcgcac
     1141 agcctgctcg tccggatcga aaccaatgta gcctaacaac tcgagcattc gcgcggtcag
     1201 atcgctgcag gtcgggtcga gaatggcatt gtggtccgca aacggcatgt cttcgagcca
     1261 atgcttcgtc acgtccttgt cgaacgcggc ccagccgcca tcgcgacact ggaagctcaa
     1321 cagccagcct agtgcgcgcg caaacaattg ctcgatcgat tgtcgatcct gcggacgcac
     1381 aagccgcagc gccatcaaca ccatcgccgt gtcgtccgtg tccggataat agatgttgtt
     1441 gtattcaaac gcccagccgc tcgcttctgg gtgtgggttg tttattgccc aatcgccgcg
     1501 gatgcccact tctttgtcga ccagccattt cgccgctcgc tgtaaggtgg gcgactccgc
     1561 gctcacgccc gattcggtaa gcgcaatcat gttaatggcc gtgtcccaga ccggcgatag
     1621 gcagggctgg atccggaaat cttcctgatc atcaacaaac agcgcggcga aatccttgcg
     1681 cgctttttcg taaatcgggt gcgtcttggg atagccaagg gcgcgcagtg cgatgagaga
     1741 gttgagcatc gccggataca ccgcggccaa gccgtcgctg ccttccccaa tgcgctcgat
     1801 catccagcgt tcggcttctt ccaaggcaac gcgccggagc ggatgcaccg gtagccggtc
     1861 aacgaacttc aaaacacgat cagagcgaag gaagaagttc cgccacgtga aaaaacgcgc
     1921 gtcgcggcgg agcgtgaaat ccttatgctc ggtgccgagc ggataaagct cgtgtaattg
     1981 tttcagcccc gggagctcac ggaccggttt gaaatgatta ataatcgcga gcggcacgag
     2041 catggcccgg ctccacgagg acatctggta aatattgaac ggcgcccatg agggtagaag
     2101 gatcatctcg accggaatgg ccgggagata tttccacgga aactggccca gcagcgcgag
     2161 gtagaatttg ctgaacgtgt tcaggcgcgg aacgccaccc aggcgcaaga tgttcgcgcg
     2221 cgcttcctgc atgaacggcg cgtcggggga atgtcctgcg agttttagcg cgaaataggc
     2281 tttcaccgac gcgttgatct cgctcggccc cgcaaagtaa atgttccagc cgccgtcggg
     2341 cagttggcgc ttcagaatgt gacggacgca gcgttgctga agttgctcat ccacttcgcc
     2401 gaaccagtgc atgaagagca cgaagtcgga gcaaagcgtg ctgtcgacga tcaactcgcc
     2461 gcaccagtgc ccgtccggcc gctgttgccg aagcagattc tcctgcgcgc gatgcatggc
     2521 ctgccagatc tcagcgtcgg tcgcggagta ggcgtgcgcg gcatccggcc ctgcgctggg
     2581 caatgcaatc acgtcagtcg aacggctcat caattctcga tatacctaac gaacgagcga
     2641 ttgtcgatct tacgatgcct gcgcggatgc cgatgcgttt ttgcccgtga gatgaccgtt
     2701 tccgcagctg gcgccgttaa acaccacgac ttcgctgcca ttgccctttg ctttcggtcg
     2761 aggaccaaag ttaaacttga tgttcttcca agtatcaccg cgttgcgcct gcaatcccag
     2821 ggtcgcggtc ggttcgtagc cgcaatggac catgcagttt tcacaacggc tgtcgcgtgc
     2881 gacgccgtcc acgacgccat accggtccca ctgcaccttc tcgagcaact cggtgtagct
     2941 ggagtaatgc gcgtccgtca tcaaatagca cgggcctttc cagccgcgaa tgttgcgcgt
     3001 cgggatggcc caggccgagc aggtcaggtc ccgtttgccg gcaaggaact caaagtaaat
     3061 cggcgtaccg aaaaacgtgt agcgattcat ccactcgtcg attttttgga acttctgcac
     3121 ggtcatgtcg cgggtgagaa agaaattttc cggccgcaag ctcagccgct taatcatgtc
     3181 cttcttggcg gcg
//
LOCUS       DASTDG010000090         9195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1096237, whole genome shotgun sequence.
ACCESSION   DASTDG010000090 DASTDG010000000
VERSION     DASTDG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096237"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1347
                     /locus_tag="VFO30_02775"
     CDS             259..1347
                     /locus_tag="VFO30_02775"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET9418238.1"
                     /translation="MAVQLPLDHLPEVWPKEFRAARIGALLHPASVSSKLEHTSRILE
                     RLNGDLFRLVAFFGPQHGYLGQTQDNMVEWKSYEHPRLRIPVHSLYGEHREPAPEMLR
                     GIDVLLVDLQDVGARYYTFIWTLYLCMRACETHGVAVVVLDRPNPLNGLTTEGPVLDP
                     AYQSFVGMHPIPVRHGRTIGELGQQFRDEAFPKCRLSILPMRNWDRAMWFDQTGLPWV
                     MPSPNMPTLDTATVYPGMCLLEGTNISEGRGTTRPFEIFGAPFIDAQTLCRELNHLKL
                     PGVFFRENYFQPTFNKFAGELCGGAQLHVLDRDAFRPFQTGVEIIRTIRKLYPESFAW
                     KQPPYEYERKKLPIEILLGAPLRNFFTD"
     gene            1344..2072
                     /locus_tag="VFO30_02780"
     CDS             1344..2072
                     /locus_tag="VFO30_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418239.1"
                     /translation="MRPQPGGVVATALWAVGRRRATGTWLQRLSIGIITVSILPNVRA
                     DIPWPEVVQRLAYENEKLARRPQGHNGEYFVVCTLYYTPMESGFTFERGFDATPVTKP
                     GLRGRTYARDFLRSVKKEGFGRIRAPVNGRYYIRYNGGDSFAFASKPTGGGGVLIARF
                     SAAAKSSQTALHRGIVIDTAAPEVRRVFGSTRWKIMDTGGGLRRWQIDCYYGEDEPLG
                     PGKFMARPRGTTFEYAYSQAKVVR"
     gene            2074..2817
                     /locus_tag="VFO30_02785"
     CDS             2074..2817
                     /locus_tag="VFO30_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HET9418240.1"
                     /translation="MRPFPVRLTFHGDLDFFLRSKSSRSVERALSERTSIKDVIESCG
                     VPHPEVDLILIDGKPEHFNCVLESEANVEVYAVSVDRRTFFPEKRLQVTEIKKFVADG
                     HLGKLARDLRLLGLDVLYDPAAEDRQLLDTAKIENRALLTRDRRLLMHAVVQHGYCVR
                     SQQPLEQTAEVLRRFDLAGVLAPFTRCLRCNARLESVKKAVVVEQLEPLTKIYYDEFR
                     RCAACGQIYWRGSHYAKLQARVAQLTQRL"
     gene            2831..3973
                     /locus_tag="VFO30_02790"
     CDS             2831..3973
                     /locus_tag="VFO30_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET9418241.1"
                     /translation="MNRLRALVFVSLGFCTRSALAYGPTGHEIVGGISDKLLANTLAG
                     AQVSSLLDGMTLEKAAVIADEIKAWDKDGADDANAFPHYPDHPKIDKQLREFWRANQP
                     TPDRASQVPSHHWFHYTDVPVLNAQKYADGRTGRSQWDIVHMIPYCVDVVRGEVSENN
                     PRKITKSVALILLAHYVADIHQPLHVGAAYFDQTGRAVDPDRSKTALEDEGGNTIMLR
                     LSHGTAQELAKRGLKLHGFWDNEAVLANLGPISPAVPKEAKFQAVDKPKRALIDQLMH
                     QQPAGWQLPSNFPLAHYAEAWADEILPLAREAHERLRFINVHPQLDEDRTVAAGTALE
                     KTARDRASYADWAAAVVRQELHKAGWRLADLLSQALTSTSLEPSRR"
     gene            complement(4113..4442)
                     /locus_tag="VFO30_02795"
     CDS             complement(4113..4442)
                     /locus_tag="VFO30_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418242.1"
                     /translation="MIKIIGTAVLAAAMLISSPAFAGGKAGCCMKNAANEQKSTSCMN
                     LTVADLNLTADQKTKLEKWQHECCQAGCTKGSHAKFLKKAKGILSADQYAKLQAECDK
                     SAKKTEA"
     gene            complement(4497..5825)
                     /locus_tag="VFO30_02800"
     CDS             complement(4497..5825)
                     /locus_tag="VFO30_02800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HET9418243.1"
                     /translation="MILVAFALPGCGRSSEKAHAAQVAKRVRSEFLHAWSNYERYAWG
                     HDALRPLSKTAHDWYGQSLLMTPVDALDTLILMKLDAEATKAQALVVNNLSFDRDIYV
                     KNFEITIRLLGGLLSSYQLTGDKRLLSLAEDLGNRLLPVFNSATGLPYVYVDLRTAAV
                     RDPITNPAETGTLLLEFGTLSKLTGKPVFYDKAKRALVETFKRRSPIGLVGQGINVET
                     GTWTDPDSHVSGGIDSYYEYLWKCWRLFGDEDCRDMWNASIPAVHKYLADEVDGELWY
                     GHADMRTGKQTETTYGALDTFFPALLALSSDIARARRLQASSFKMWNLYGIEPEALDY
                     KTKRIVSGSYALRPEIVESTYYLYHRTRDPKYRRMGEKMFDDFVRYCRAEGGYAALAD
                     VVTKQPRDEMESFVLAETFKYFYLLFAPAGTLDFDKVTFNTEAHPLRRTW"
     gene            complement(5863..6780)
                     /locus_tag="VFO30_02805"
     CDS             complement(5863..6780)
                     /locus_tag="VFO30_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET9418244.1"
                     /translation="MILTCNEGEREFSSVAALYERRINSSDGHRPPLQQSYISTVVLG
                     FLKERRRNELKTKPFPSEWLDIIRRRVAFFARLSKADQAELLGHIQVFLAEKRFEGCA
                     GFEIIDEIRVTIAAQACLLLLHRKTDYFPRLLTILVYTSTYLVDEQRHIEGPIWEEGK
                     MSRLGETGRTMRSMVLAWDAVQSGADDPSDGQNIVLHEFAHQLDYENDAADGAPALAT
                     HEQQLSWREVMTTEFASLRAADDTGIPTLLNTYGATNPAEFFAVAVEAFFEQPAALRS
                     THPRLYKELRRYFNQNPIEYSAERRIWRD"
     gene            6803..7924
                     /locus_tag="VFO30_02810"
     CDS             6803..7924
                     /locus_tag="VFO30_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9418245.1"
                     /translation="MNTYSISRRQFAHLLGVGAAAAIVRPRITIATESSTLPAAGKAG
                     VVRLSANENPYGPSGKAHEAMKDAHNFCNRYPDEANDVLIDKIAKINSVNRKQIALGD
                     GSSEILKMCAEAFTGPTQGKLIVADPTFEAILEYAKANGAEVVKVPLTSRFAHDLPKM
                     AAAARRGLIYVCNPNNPTASITPKDDLRDFIDKTPQETMILVDEAYFHYADSPDYESM
                     IPLVKDHPNLIVARTFSKIYGMAGLRCGYCVAQRSTIERIHAFQMWDSVNTMALAAAS
                     ASLEDVDQVNDGRRLNTEAKSFTVSELEKLGYKSIPSQANFIMFDCKRPVVPIITAMK
                     ERNVAVGRLFPALPTHMRLTIGKKSEMESFLSAFKQVIA"
     gene            complement(8011..8388)
                     /locus_tag="VFO30_02815"
     CDS             complement(8011..8388)
                     /locus_tag="VFO30_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418246.1"
                     /translation="MRVRFNTKIRFNGREYASADEMPPSVREAYTRAVGETAVLRSGA
                     RLADKLNAKVIVNDKEFSSPGELPVGERRLLQDALAALLPNNLALSANEARRIRQKKM
                     IRALVVAIGAAGFVYLWLHGFFG"
     gene            complement(8475..>9195)
                     /locus_tag="VFO30_02820"
     CDS             complement(8475..>9195)
                     /locus_tag="VFO30_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9418247.1"
                     /translation="ALAEFLFDDENAMIRIDMSEYMEKHTVARLIGAPPGYVGYEEGG
                     QLSEAVRRKPYSVVLFDEIEKAQQDVFNVLLQVLDDGRITDGRGRTVDFKNAVIIMTS
                     NIGSQFITEEESREARSRLVMDALRAHFRPEFLNRVDEIIIFDRLTEDDLKKIVEIQL
                     RRLSKRLEQQKITLDLSDSAKQLLAREGYDPVYGARPLRRTIQKEILDPLSIDILEGK
                     VREGQTVHVEARDGALAFNAR"
BASE COUNT         1995 a         2629 c         2585 g         1986 t
ORIGIN      
        1 tattgacttt catgttggga caaacaccga gtcgggttgt atgcaaaaaa agccataatt
       61 gcgaggtttt tcttcaggcg tccggagaat ctcacaggca tcccaacacg gccctgacta
      121 agattaggcg agcgttgcag cgattgtgga tcggccgcgc cgcaggcgat gctaaataag
      181 tgcggcttcg ccgcgcaagt taccatcgag cgccggagcg ctcgatccac ctcggtccat
      241 gtgttaaaaa cgtgcgacgt ggctgtccaa cttccactcg atcacttacc cgaggtttgg
      301 ccaaaagaat tccgtgccgc ccgcattggt gcgctgctgc atccggcgtc tgtgtcatcc
      361 aagctcgagc acacctcgcg aattctcgag agactgaacg gcgatttgtt ccggctggtc
      421 gcgttcttcg gcccgcagca tgggtatctc gggcaaacgc aagacaacat ggtcgaatgg
      481 aaaagctatg agcacccgcg attgcggatt ccggtgcaca gcttgtacgg tgagcatcgg
      541 gagccggcgc cggagatgct gcgcggcatc gatgttttac ttgtcgatct tcaggatgtc
      601 ggcgcgcgct actacacgtt tatctggacg ctctatctct gcatgcgcgc gtgcgaaacg
      661 catggagtcg ctgtcgtcgt gctcgatcgg ccaaatccac tcaatggcct aacgactgaa
      721 gggccggtgc tcgatccagc ttaccagtcg tttgtcggca tgcatccgat cccggtgcgc
      781 cacggccgta ccatcggcga gctcgggcag cagtttcgtg acgaagcatt tccgaagtgt
      841 cgattgtcga tcttgccaat gagaaattgg gaccgcgcaa tgtggttcga ccagaccggc
      901 cttccgtggg tgatgccgtc gccgaacatg ccgacgctcg acaccgcaac agtttatcct
      961 ggcatgtgtt tactcgaggg cacaaacatc tccgagggac gtggtacgac gcgcccgttc
     1021 gagattttcg gcgcgccgtt catcgatgcc cagacgcttt gccgcgagct caatcaccta
     1081 aagctgccgg gcgttttctt ccgcgagaat tacttccagc cgaccttcaa caaattcgcc
     1141 ggcgagcttt gtggcggcgc gcaactgcac gtgctcgatc gcgacgcgtt tcgcccgttt
     1201 caaaccggcg ttgaaatcat ccgcacgatt cgaaagttgt atcccgagtc attcgcgtgg
     1261 aaacagccgc cttacgagta cgagcgcaaa aaactgccga tcgaaattct actcggggcg
     1321 ccgctccgga atttcttcac cgattgaggc cacaacccgg aggagttgta gccaccgccc
     1381 tgtgggcggt cggacgcaga cgtgccacag gcacgtggct acagcgctta agcattggaa
     1441 tcatcaccgt gtcgatcttg ccgaatgttc gagccgacat tccgtggccg gaagtggtgc
     1501 aacggctcgc ctatgaaaac gaaaagctcg cgcggcgacc gcagggtcac aacggcgaat
     1561 acttcgtcgt ctgcacctta tattacacac cgatggaatc tggcttcaca ttcgagcgcg
     1621 ggtttgacgc gacgccagtc acgaagccgg gtctgcgtgg ccgcacctat gcgcgcgatt
     1681 ttctgcgctc cgtcaaaaag gaaggctttg gtcggatcag agcaccggtg aacggtcgct
     1741 actacattcg ctacaacggc ggcgattcgt ttgcctttgc ttcaaaaccg accggcggcg
     1801 gcggcgttct catcgcgcgg ttctcggccg cggcgaagtc cagccagact gcattgcacc
     1861 gcggtattgt catcgatacg gcggcgccag aagtgcggcg cgtcttcggc agtacccggt
     1921 ggaagattat ggataccggc ggagggctgc gacgttggca gattgattgt tactacggcg
     1981 aagacgagcc cctggggcca ggcaaattta tggcgcggcc gcgcggcacc accttcgagt
     2041 acgcctattc ccaggcaaaa gttgtgcgat aacgtgaggc cattccctgt tcggctcacg
     2101 tttcacggcg acttagattt tttccttcgc tcgaaatcaa gtcgatccgt ggagcgggca
     2161 ttgtcggagc gcacgtcgat caaagacgtg atcgagtcgt gcggcgtacc gcatccggag
     2221 gtcgatctaa tcttgatcga cggtaagccg gaacatttca actgcgtcct tgagagcgaa
     2281 gcgaacgtcg aggtatatgc agtcagtgtg gatcggcgca cgttttttcc agaaaagcga
     2341 ttgcaagtca cagagatcaa aaagtttgtg gccgacggac atcttggaaa gcttgctcgc
     2401 gatttgcggc tacttggtct cgatgttctc tacgatcctg cggcggaaga tcgacaattg
     2461 ctcgacacgg cgaagatcga gaatcgcgcc ctgctcacgc gcgatcggcg tctgctcatg
     2521 cacgcggtcg tgcaacacgg ctactgtgtc cgctcgcaac agccgctgga gcaaacagcg
     2581 gaagtgctgc ggcgttttga tctcgctggc gtgctggcgc cattcactcg ctgcttgcgt
     2641 tgcaacgcgc gactcgagtc ggtcaaaaaa gcggtggtag tcgagcaact cgagccgctc
     2701 acaaagattt actatgacga gttccggcgc tgtgccgcat gcggccaaat ttattggcgc
     2761 ggctcgcatt acgcgaaact gcaggcacgc gttgcgcagc tgacgcaacg gttgtagttc
     2821 tcccgcgatt ttgaaccggt tgcgtgcgct cgtattcgtc tccctcggct tttgcacccg
     2881 ctctgcgttg gcctacggac cgaccggaca tgaaatcgtc ggtggcattt ccgacaaact
     2941 gctggccaac acgctcgccg gtgcacaggt gagttcgctg ttggacggta tgacgctgga
     3001 aaaggcggcg gtgatcgccg acgagatcaa ggcgtgggac aaagatggag ctgatgatgc
     3061 aaacgcattt ccccattatc ccgaccatcc gaagatcgac aaacaattgc gggagttctg
     3121 gcgcgcaaac cagccgacgc ctgatcgcgc atcgcaagtg ccgtcgcatc attggttcca
     3181 ttacaccgac gttccggtgc tgaacgcgca gaagtatgcg gatggcagaa ccggtcgcag
     3241 tcaatgggac atcgtccaca tgattccata ctgcgtggac gttgtgcgcg gcgaggtttc
     3301 cgagaacaac ccgcggaaaa tcacaaaatc cgtcgccttg attttgctcg cgcactacgt
     3361 cgcggacatt caccagccgc tgcatgttgg cgcggcgtac ttcgaccaaa ccgggcgggc
     3421 ggtggatcca gacagaagca aaacggcgct cgaagatgaa ggcggcaata cgatcatgct
     3481 ccggctcagc cacggcacag cacaggaact ggcaaagcgc gggttaaaac tacacggctt
     3541 ctgggataac gaagccgtct tggcaaacct gggaccgatc tctccggccg tgccgaagga
     3601 agcgaagttt caggccgtag acaaacccaa gcgcgcgtta atcgaccaac tgatgcatca
     3661 gcagccggcg ggctggcagt tgccgtcaaa tttcccgctg gcacattatg ccgaggcttg
     3721 ggccgacgaa attttgccgc tcgcgcgtga ggcacacgaa cggctccggt tcatcaatgt
     3781 gcatccgcag ctcgacgagg accgcaccgt cgcagccggc acagcactgg agaaaactgc
     3841 tcgcgatcga gcgagctacg cggactgggc cgcggctgtt gtacgtcagg aattgcacaa
     3901 agcgggttgg cgattggccg atcttctcag tcaggctctg acgtcaacgt cactcgagcc
     3961 gagtcggcgc tgatcagctt agcgaatgcc gcttccgatt tacccgcggc ggaagcaaat
     4021 cgtggtcgcc atgccgagcg cgatttgcaa gattgacaag tgtagccacg gccatgtcgg
     4081 ctgtccctgc gcaatgtcgg cggctacgtt gattacgcct ctgtcttctt cgccgatttg
     4141 tcgcactccg cctgtagttt ggcgtactgg tcggcagaga gaattccttt cgctttcttc
     4201 aggaatttag cgtggctccc tttcgtgcag cccgcctggc aacactcgtg ttgccatttc
     4261 tcgagcttcg ttttctggtc ggccgtcaaa ttcaagtcgg cgacggttag gttcatgcac
     4321 gaagtcgatt tctgttcgtt tgccgcattt ttcatgcagc aaccggcctt gccgccggca
     4381 aacgcgggcg agctgatcag catcgctgcc gcgagaactg ctgttccgat aattttaatc
     4441 atggtgttca ttagatacgc gaggagcctt tggcgttcaa attgccgtcg gcgctctcac
     4501 catgtgcgtc gcagcggatg ggcttcggtg ttgaacgtca ctttgtcaaa atcaagcgtc
     4561 cccgccggcg cgaagagcag gtagaaatac ttgaacgtct cggccaggac aaaactctcc
     4621 atttcatcgc gcggttgttt cgtgaccacg tcagccaggg ccgcatagcc gccctcagca
     4681 cggcaatacc taacaaaatc atcaaacatt ttctcaccca ttcgccgata tttcggatcg
     4741 cgcgtgcggt gatacaggta ataagtcgac tccacgatct cgggacgcag agcgtaactg
     4801 ccagaaacga tgcgcttggt tttgtaatct aatgcttcgg gttcaatccc gtagaggttc
     4861 cacattttga atgaggaagc ttgcagccga cgtgcgcgcg cgatgtcgct agaaagtgcc
     4921 agcaacgcgg ggaaaaatgt atcaagcgcg ccgtaagttg tttcagtttg cttgccggtc
     4981 cgcatgtcgg cgtggccgta ccagagttcg ccgtcaactt cgtcggccag atatttatga
     5041 accgccggaa tactcgcatt ccacatgtcg cgacaatctt cgtcgccgaa caagcgccag
     5101 cacttccaga gatattcgta ataagaatcg atgccgccgc tgacgtgact gtctgggtcg
     5161 gtccatgtgc cggtctcgac gttgatgcct tgaccgacga gtccaatcgg cgagcggcgt
     5221 ttgaacgttt cgaccaaagc gcgctttgct ttgtcgtaga aaactggctt gccagtcagc
     5281 ttgctcagcg tgccgaattc cagaagcagc gtgccggtct cggccggatt cgtgatcgga
     5341 tcgcgcaccg cggccgtgcg aagatcgaca tatacgtaag gcaaaccggt cgccgaattg
     5401 aaaaccggaa ggagtctgtt ccccaaatct tcggccaaac tgagaagccg cttatccccg
     5461 gtgagctgat aactggagag caatccgccg agcagacgaa tcgtgatctc gaagttcttc
     5521 acataaatgt cgcgatcgaa ggagagattg ttcacgacga gcgcctgcgc ctttgtcgct
     5581 tcggcatcga gcttcatcag gatgagcgta tcgagtgcat cgaccggggt catgagcagt
     5641 gattgtccgt accaatcatg tgccgttttg ctcaacggcc gcagagcgtc atgtccccag
     5701 gcgtaacgct cgtaattgct ccaagcgtgc agaaattcgc tgcgcacgcg cttggcaact
     5761 tgcgccgcgt gcgccttctc agatgagcgg ccgcatcccg gcaacgcaaa cgcgaccaag
     5821 atcacgacgc acaatccaac ctcgcgtaga gaaagggtca catcagtcgc gccagatcct
     5881 gcgctcggcc gagtactcga tcggattttg attaaaatag cgccgcagct ccttgtagag
     5941 tcgcggatgc gtagagcgca gcgcggctgg ttgttcaaag aaagcctcga ctgctacggc
     6001 aaaaaactcc gccggatttg tcgcgccata cgtattgagc aaggtcggaa ttcccgtatc
     6061 atcagccgca cgaagtgagg caaactccgt cgtcatcact tcgcgccagg acaattgctg
     6121 ctcatgtgtc gccaatgctg gcgcgccgtc cgcggcgtca ttctcataat cgagttggtg
     6181 agcaaattca tgcaggacaa tattctgacc gtcggaagga tcgtctgcgc cggattgcac
     6241 ggcatcccac gcgagcacca ttgatctcat cgtgcggccg gtctcgccga gtcgagacat
     6301 ttttccttct tcccaaatcg gaccttcgat gtggcgctgt tcgtcgacca aatatgtcga
     6361 tgtgtagacg agaatcgtaa gcagtcgcgg gaagtaatca gtcttgcgat gcaacaacag
     6421 caaacaagcc tgggctgcga tcgttacccg gatttcgtca atgatttcaa agccggcaca
     6481 gccttcgaaa cgcttttcgg ccaaaaatac ctggatgtga ccgagaagtt ccgcctggtc
     6541 agccttcgaa agccgggcaa aaaacgcaac cctgcgcctg atgatatcca gccactcgct
     6601 cggaaacggc ttcgtcttta gctcgtttcg gcgtcgttct ttcagaaagc caagcaccac
     6661 cgtggaaatg taactttgct gtagcggcgg tctatgaccg tcggacgaat taatgcgacg
     6721 ctcgtagagc gccgctacag aagaaaattc acgctcgcct tcattgcagg tgaggatcac
     6781 aatttccgag gaggagcctc ttatgaacac atattcaatc tctcgtcgtc agtttgctca
     6841 tcttttaggt gttggcgcag ctgctgcgat cgtgcgaccg cgaatcacaa tcgcaacgga
     6901 atcttccacc cttcctgctg cgggcaaagc cggcgttgtt cgactcagcg cgaacgaaaa
     6961 tccgtacgga ccgtcaggca aggcccatga agcgatgaaa gacgcgcaca acttttgtaa
     7021 ccgctatcct gatgaagcga acgatgtctt gatcgacaag atcgcgaaga tcaacagcgt
     7081 gaatcggaag cagatcgcac tgggcgatgg ctcgagcgaa attttgaaaa tgtgtgccga
     7141 ggcgttcact ggcccaacgc aaggcaagct gattgtcgct gacccaacct tcgaagccat
     7201 tcttgaatat gcaaaagcga acggcgccga agtcgtcaag gtcccgctaa cgagcagatt
     7261 tgcgcatgat ctgccgaaga tggctgctgc cgcgcgacgc gggttgattt acgtctgcaa
     7321 tccgaacaac ccgaccgcga gcattacgcc gaaggacgat ttacgcgact tcatcgacaa
     7381 aacgccacag gagacaatga ttcttgtcga cgaagcttac tttcattacg cggatagccc
     7441 cgattacgaa agcatgattc cgctcgtcaa agatcatcca aacctgatcg tcgcgcgaac
     7501 gttttcgaaa atctacggca tggctgggct gcgctgtggt tactgcgtag cgcaaagatc
     7561 gacgattgaa cggatacatg cgtttcaaat gtgggacagc gtcaacacga tggcactggc
     7621 cgcggcaagc gcgagcctcg aggatgttga tcaagtaaac gatggtcgac gattaaacac
     7681 tgaagcgaag agtttcacgg tcagcgaatt ggagaagctc ggctacaaat ccattccatc
     7741 acaggccaac ttcatcatgt tcgactgcaa acggccggtc gtgccaatca tcacggccat
     7801 gaaagagcgt aacgtcgccg tcggccgcct cttcccagcc ctgccgactc acatgcgatt
     7861 gacgatcggc aaaaaatccg agatggaatc gttcctgagc gcgttcaagc aagtgatcgc
     7921 gtaggtgcaa cgcgatctcc cagcgcgttg ccaacatagt gcggccctcg gcgcacggat
     7981 taacatcgcg ctcggagagc tcgatccacc ttagccgaag aatccgtgga gccagagata
     8041 aacaaatccg gcggcgccaa tagcgacaac cagcgctcga atcattttct tttgcctgat
     8101 tcttcgcgcc tcgtttgcag acagggcgag attgttaggc aaaagcgcgg cgagcgcgtc
     8161 ctgcaacaat cggcgttcac cgacgggcag ttcccccggg ctactgaact ccttgtcgtt
     8221 cacaatgact ttcgcgttca gtttgtcagc cagtctcgcc ccggaacgca gcaccgcagt
     8281 ctcgcctacg gcgcgcgtgt aagcctcgcg cacgctcggt ggcatctcgt ccgcgctcgc
     8341 atactcgcgg ccgttgaaac gaatcttcgt attgaaccta accctcacgt cacagtcctg
     8401 ggcagcaatt tgcctaccaa cctagctcag cctccgcggt ttagaggcag aactcccgct
     8461 gacgcgccca gttgctagcg ggcgttgaaa gccagcgcgc cgtctctggc ttcgacgtgg
     8521 acggtttggc cttcacggac tttcccttcg aggatgtcga tactaagcgg gtcgagaatt
     8581 tctttttgga tggtgcggcg gagcggacgc gcgccgtaaa cgggatcgta accttcgcgg
     8641 gcgaggagtt gtttggctga atcagaaaga tcgagcgtga tcttttgttg ctcgaggcgt
     8701 ttcgacaaac ggcgcagttg aatttcgacg atcttcttca agtcatcctc ggtgagtcgg
     8761 tcgaagatga tgatctcatc gacccggttc agaaattcgg gccggaaatg cgcgcgcaac
     8821 gcgtccatta ccaggcgcga gcgcgcttcg cgcgattctt cctcggtgat gaattgcgag
     8881 ccgatattcg aggtcatgat gataacggcg ttcttgaaat cgaccgttct cccccgcccg
     8941 tccgtgatcc ggccgtcatc gagcacttgc aggagcacgt tgaatacatc ctgctgcgct
     9001 ttttcgatct cgtcgaagag cacgaccgag tagggcttgc gccggacggc ttcggaaagt
     9061 tgaccgcctt cctcgtaacc gacgtaaccc ggcggcgcgc cgatcagtcg cgcgaccgtg
     9121 tgcttctcca tgtactcgct catgtcgatc cggatcatgg cgttctcgtc gtcgaacaaa
     9181 aattccgcga gcgcg
//
LOCUS       DASTDG010000091        18538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_724359, whole genome shotgun sequence.
ACCESSION   DASTDG010000091 DASTDG010000000
VERSION     DASTDG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 18538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18538
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724359"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFO30_02825"
     CDS             <1..807
                     /locus_tag="VFO30_02825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9418248.1"
                     /translation="LPEGEWLERVAQPLRQDRQLLAQLTERFWPGPFTVVLPKRQVIP
                     DLVTAGLNTVAVRISAHPIFAEVVRTFGKPLAAPSANRFGRLSPTTAQHVHDELNGRI
                     PLIIDGGPTEHGIESTIVIVRDGKIDILRRGSISYEQLAEFAEVNVVSTTGKISVPGQ
                     LKSHYAPNTPLCLVDNAANFASENLRVGLLAWNPVTDQNRFAATRLLSERRDYREAAA
                     NLFRYLRELDALDLDLIVAERVPAQRIGAAILDRLERAAHDSGQTRDRLE"
     gene            complement(701..2377)
                     /locus_tag="VFO30_02830"
     CDS             complement(701..2377)
                     /locus_tag="VFO30_02830"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9418249.1"
                     /translation="MKYIFITGGVVSSLGKGLAAASLGTLLESRGLRVVLQKFDPYLN
                     VDPGTMNPFQHGEVYVLNDGAETDLDLGHYERFTNCVLSRHNNLTSGQVYESVILKER
                     RGDYLGKTVQVIPHVTDVIKDRIRELSDESKYDVVITEIGGTTGDIEGLPFLEAIRQF
                     ILEVGTQNVVNMHVTLVPYIKAAAELKTKPTQQSVAKLREIGLQPQVLICRTEKPLER
                     ELRQKLSMFCSVSEKAVIEARDVQHTIYEVPLMLHSEGLDELVCNLLQLQTPAPELAS
                     WRKFVECVINPHERVKIAVVGKYIHLQDAYKSIYESLTHAAASQDCGIDLKLVDAESL
                     QDRVGTQLKDVAGVLIPGGFGDRGAEGKIKAARYAREHQIPYLGLCLGMQVATIEFVR
                     DVCGIAKANSTEFDPNTPEPVISLLEEQREKIRSKGGSMRLGTWPTKIVPGTLAERIY
                     GEREVMERHRHRYEFNMKYRDRMTEKGFVISGTSPDDTLAELIELRDHPYFVGCQYHP
                     EFQSKPNKPHPLFKGFIQACLAFDLNRAPRVPVDRESRPRSSEPELVLQR"
     gene            complement(2374..3123)
                     /gene="kdsB"
                     /locus_tag="VFO30_02835"
     CDS             complement(2374..3123)
                     /gene="kdsB"
                     /locus_tag="VFO30_02835"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET9418250.1"
                     /translation="MSKAVGIVPARWRSTRFPGKPLHLIADKPLLRHVWERAKEAKNL
                     GAVMIATDDMRIAEAAFSWGAEVALTSPRHQSGTERVAEVAAKARQFALIINIQGDEP
                     LIDPKLIDSIVQKLRSDRKIDIVTAAHPFEDPVEAVSPHQVKVVVDLASRALYFSRFP
                     IPFVRGKSTRILRHQGIYGFRRPALLRFVKWKPTSLERAESLEQLRALENGVRVYVLV
                     TKHGSPGVDTPADAKALERKLARAARTAVSR"
     gene            complement(3204..4211)
                     /locus_tag="VFO30_02840"
     CDS             complement(3204..4211)
                     /locus_tag="VFO30_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9418251.1"
                     /translation="MKLRRLEHILDRAPAKRLLVIGDLMLDEFVWGKVGRISPEAPVP
                     VVQVTDESFVPGGAANVARNLREFTKRVSVAGMIGHDDSGDRLRELLAGEMIKTLNST
                     VNKNFRTIVKTRIIARHQQVVRVDREQIAGPSREHLKKIVADVAKQLANIDAVIFEDY
                     GKGFLSDELVTQVTHAARAQGKIVTADPNATRLLKWRGMTAVKPNRTEAFLAAGIPYQ
                     DPSESLERDVELARVGKILLRRWDAQNVLITLGEQGMMLFQPGQPPHHIPTRARQVFD
                     VSGAGDTAIALFTLALTCEATPLEAAEIANHASAVVVGKLGTATVTPDELIASFRNDA
                     D"
     gene            4370..5167
                     /locus_tag="VFO30_02845"
     CDS             4370..5167
                     /locus_tag="VFO30_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418252.1"
                     /translation="MIPVSKFLKVWAFATMGSALCAHGAPPPQHIDVKQLSRKVEDVV
                     VPLPNEVFGALNKLGSVNWKEYVRTNKGSNFTERPRIALLLGTVIADGFIAVQAEDAP
                     AVRDIGQRVQTLARGIGVERSITPHAKAITEAADKRNWEGVRQELDRTQNSVQQAMNE
                     VHDEKLSQLVSLGGWLRGTQVLTAVVTKHFSAEGAELLHQPDLVVYFQTRLEAMPEFN
                     LPIVREIQGALVQVKPLIDVGNARISPASVKKINEITTRLDKGIVTR"
     gene            5190..5627
                     /locus_tag="VFO30_02850"
     CDS             5190..5627
                     /locus_tag="VFO30_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418253.1"
                     /translation="MKFALRGSLFAAVLLGMHTALCGSVDDAQSFALQAAEPYVKEGF
                     QVREDYWGGDLGSGEKKAVRQQLFKGNEYWFWLGTDVGRARISVHIYDSDGKLAEESD
                     GWQKGHFAAAHIIPKATGSYFIIVAVEESPEERTHWALVYGFR"
     gene            5691..6665
                     /locus_tag="VFO30_02855"
     CDS             5691..6665
                     /locus_tag="VFO30_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9418254.1"
                     /translation="MTETRTLQFESARALQSLYANDLKLLKNLEESLGVKVTTREGWV
                     KLEGEPDKIDKAQQVFDQLEQARQKGVPIQKHEFNYALNSVSEAREENLGDLAATKIL
                     TSPRKLPIMARSAGQRDYVEAIQKHDIVFGIGPAGTGKTYLAVAMAVAALKKEQVARI
                     ILTRPAVEAGEALGFLPGELEEKIMPYLRPLYDALRDMLETEEVERLVARQTIEIAPL
                     AYMRGRTLNNAFVILDEAQNTTTEQMFMLLTRIGPNSKCVITGDITQIDLPTNKHSGV
                     LEALQALKYVPGIAIIHFSERDVVRHELVRAIINAYQQHRAPLDSAQD"
     gene            6787..8307
                     /locus_tag="VFO30_02860"
     CDS             6787..8307
                     /locus_tag="VFO30_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9418255.1"
                     /translation="MSIFAAFITGLAFLIFSGQQPEPTKNFVIAILFLAIAITQLWIN
                     QPNTFAQNSRVLLVFGTIFVQLALTKLLYVLCAKQTFQFLTPEVAGLIAPYALAPLAL
                     SVLLGRNHGLYAAVSVSLWSSVLFRKIDAPLLVTSLISGFTAVYLTLQVRRRSRLVRA
                     GVGVGVAIWLLSLTFGLIGPINVFSPMANDWGMIGVQSALAIGNGIVTAMIVGGALPM
                     LEHLFQVTTDISWLEASDLNHPLLRRMTIEAPGTYHHSLVVANLAEAAAEAIGANATL
                     CRVCSYFHDVGKLVKPQYFTENMNFERNPHDDLAPTMSALIILSHVKEGVDLALKHKL
                     TQRIIDIIQEHHGTSLISYFYQRGLQQQADARAGGKIMNIRAEDIPEVREESFRYPGP
                     KPQTKESAIVSLADIVESASRSLEKPTPQKIEQLVHELIEERIADGQLDECDLTLGDL
                     RTISDRFRFTLMTMLHTRIAYPKQEPRIAGPRDETLRPDVMAATRRPPSAPPVSAA"
     gene            complement(8466..9041)
                     /locus_tag="VFO30_02865"
     CDS             complement(8466..9041)
                     /locus_tag="VFO30_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9418256.1"
                     /translation="MTGQELLASLGKFLGAKIKDQSEFRGETTYTIAPEDLREIAKSC
                     RDDLSFDYLIDITSIDNFGEDPRWEIVYELYSMTLAAHLRLKLRVSEDVGAVDTVSDI
                     WPTANWHEREIYDMMGVKFNGHPDLRRILMWDGYPFFPLRKEFPLEGLPSEMPDVAFT
                     KSAPLEGGPFVTRPSTATAKDREPRARPPNL"
     gene            complement(9163..9699)
                     /gene="nuoB"
                     /locus_tag="VFO30_02870"
     CDS             complement(9163..9699)
                     /gene="nuoB"
                     /locus_tag="VFO30_02870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9418257.1"
                     /translation="MAEERSTAYDSKIEGNVIFTRFDAALNWVRKNSLWPMPMGLACC
                     AIELMATAASRFDISRFGAEVMRFSPRQCDVMIVAGTVTYKMALAVKRIYEQMPEPKW
                     VIAMGACASTGGMYRSYAVLQGIDQLLPVDVYISGCPPRPEALLAGLMKLQEKISGER
                     SLRAQKPALTKKAEEALA"
     gene            9879..10076
                     /locus_tag="VFO30_02875"
     CDS             9879..10076
                     /locus_tag="VFO30_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418258.1"
                     /translation="MNSAIRTHLDELEKQSIFIFHEAYRTFKNIAMPSSIGKDSNVLI
                     WLARKAFFGGVPFPVQHIDTT"
     gene            10294..12066
                     /locus_tag="VFO30_02880"
     CDS             10294..12066
                     /locus_tag="VFO30_02880"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HET9418259.1"
                     /translation="MNIDSARFQGHLIAPHGGELVDLKLDPENAAEIKARSRDFPSWD
                     LTSRQIRDLELLLSGGFSPLRGFMNRDDYERVCQEMKLSNDLLWPIPVTLDVPEAFAK
                     SLKPGASKIALRDSEGVMVAVLYVEDVWQPDRKAEAQAVLGTTSTVHPGVDYLLNRGH
                     GWYVGGRVEGLQLPTHYDFRTLRLTPSDLRAEFVRRGWRRIVAFQTRNPMHRAHVELT
                     FRAARQVEASLLIHPSVGVTRPGDVDYFTRVRCYQLLVSKYPRGTAKLALLPLAMRMG
                     GPREAIWHAIIRKNHGCSHFIVGRDHAGPGNDADGKPFYGPYEAQELFRKHQADIGVE
                     MVPFQMMVYVEDRDKYFPENEVPPGARVLNLSGTELRRRLNEGRDIPVWFTFPEIARE
                     LRRSFPPRHKQGLTVFFSGLSGAGKSTIANVLMIKFLEMGGRPVTLLDGDLVRKHLSS
                     ELGFSKEHRDINIRRIGYVASEITKNGGIAICAPIAPYDSTRKDVRALIEPFGGFVLV
                     HLATPIEVCEQRDRKGLYAKARAGVLKEFTGISDPYEVPTDAEVTVDTAELSPEEAAQ
                     EIILHLEQEGFIGINGTAPDPNST"
     gene            12182..12631
                     /locus_tag="VFO30_02885"
     CDS             12182..12631
                     /locus_tag="VFO30_02885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418260.1"
                     /translation="MPNERVLDLTIRAARTSDAAALARLMCELGYETTTAEMASRLET
                     ITGDPRYRTLVAVAKGKVEGMIGTFARPSFEHNDLSGRILALIVASTARRRGIGQRLI
                     AAAENDFARRNIRRVAVDTRFEREQAHEFYETLGYRRNGFRFVKTLD"
     gene            complement(12663..15788)
                     /locus_tag="VFO30_02890"
     CDS             complement(12663..15788)
                     /locus_tag="VFO30_02890"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET9418261.1"
                     /translation="MRHAEPSPVSTTTDKPKRVGFFRRCGEWLFSQRHFHFKLLSGTA
                     AGVTVIVLLAGIFLYVTIRNHQQENVRAHTIEVIRLSSLIENDIAALETGHRGFLLTG
                     KNDYTLSFDRRREAIKRRIENLNALIFDNPKQRKRIVKVQTIVQQWIDTVAVPEIKAR
                     QATSGSPDTSSPDSQPVALGNSLLDQARAILQSVQDDEQIVLNQRMLEQEWATQSTQI
                     LDFLPKLERSVLEMQKEKRGYLLTGEASFLENYKRAVSDFYTYNGYLSILVANSPSQA
                     ELLAEVRANVERWVSTCATPEIDARRDGKEINTLMISDVGEPLMADIRQMLGTLEQNE
                     VSVYEMRNSAASRERVIKTSILGCLAFLAVALLVVSNSYSFALVRRQLAKLEAVETRI
                     RSIIENILDGMVTLDEEGVICSMNPAAEKMFGYSEQEMVGYKLTKLVPKVYGDEPDAK
                     PVACAWEQIAKRTGSTILALGRTRKLVSFPLEMSLSQMQVGDNKLFVAMVRDVTERRR
                     FEKEIAAEKESLAVTLRSIGDGVITTDVQGRIIILNNEAERLTGWTSQEAIGQPLKTV
                     FNVAIDLAAQARVPRSAYRNEVQSILLGLAENATLTSRDGTERIIEQSASPIRDNKNE
                     VAGVVLVFRDITQRQRDEAERRKAETLEQLGLLAGGIAHDFNNLLTAIIGNISLASLL
                     LPPDAEMATRLVDAKNASIRARDLAQQLLTFARGGAPIKKTTSIGKLIQDTVGFSLRG
                     SHSRSEFQFGADLWPAEIDPGQISQVIANLAVNADQAMPNGGTLHVICENFRYTSATT
                     PVIPDLPPGDYIRISIRDEGVGIPEQYVKRIFDPYFTTKPKGNGLGLATAYSIIKNHN
                     GLITVESQVHFGSTFTVYLPAALHQELPIDAPVTMSPAMTGTGRVLVVDDEEAIRTLV
                     EFTLTRLGYEVKQADTALDGVNIYRERFERGERFDAVILDLTLPGGMGGKEALKKLIE
                     IDPTVNAIVSSGYATDATMSRYQDFGFRGVIAKPYEAAELGKIIHDVIASSHVNYGEH
                     IEGGRLATRVA"
     gene            complement(15869..16612)
                     /gene="truA"
                     /locus_tag="VFO30_02895"
     CDS             complement(15869..16612)
                     /gene="truA"
                     /locus_tag="VFO30_02895"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9418262.1"
                     /translation="MSQRLKLILAYDGMHFAGWQSQTHRNTIQDQLERAFDRISGQRV
                     RVQGAGRTDTGVHALGQCAHVDLAEPKLAAGDWVQALNAVLQPTIRVLRCSFVSTHFH
                     ARLSANGKVYRYRIWSGPVMPPHEYGRAWHLAAKLDFDRLERSAQEFVGMHDFAAFAA
                     NRGHTEANTTRTIRSVRSLHRGSRVNIEFDGDGFLYKMVRLMVGSIVQCALGKQTVAE
                     IANQLSRRQSAGRRFVAPAAGLFLVRVRY"
     gene            complement(16634..17053)
                     /gene="ruvX"
                     /locus_tag="VFO30_02900"
     CDS             complement(16634..17053)
                     /gene="ruvX"
                     /locus_tag="VFO30_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9418263.1"
                     /translation="MNPILGIDLGKVRIGLAISDELQLLAHPLETIPATGNAPARIAE
                     IVRQRKIDSVVVGLPRRMSGEVGDAAKSALGFADKLRAMLPCEVVTWDERLTTAAANR
                     ALRSAGKSTRRSREYLDQVAAQMILQGYLDSRRQSAM"
     gene            complement(17069..18148)
                     /locus_tag="VFO30_02905"
     CDS             complement(17069..18148)
                     /locus_tag="VFO30_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9418264.1"
                     /translation="MAISNLIYALILAGGSGQRFWPLSRRARPKQLLQLVTKRALLEE
                     TVARLDGLVPRERILVLTNVEQADAVRELLSDFPKENILAEPAKRDTAAAVALGAGWV
                     AARDHGATMLVLPADHLIANRAAFHETLSLAAEAAAQMGELVVIGVKPTWPCPGYGYI
                     EHGEAVRFQNGADKGAVHRVVRFREKPNVDLAESFLRKGNFRWNAGMFVWSVPTVLSE
                     FNRHAPELADFISQLRAPGRFEKVLGERFAKLPRISFDYAIMEKADRVLVVEASFDWD
                     DIGSWQAVANYFKRDIDGNAANCPLVALESTHNIIFAEDETTVALLGVHDFIVVRTGD
                     AVLVCHRHDVEKIKNLVTKLPPELQ"
     gene            18248..18529
                     /locus_tag="VFO30_02910"
     CDS             18248..18529
                     /locus_tag="VFO30_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418265.1"
                     /translation="MLKSSDQLPLDALLTLTRTAFGLGIGMLMADKIKRPVRQAAGIT
                     LLSIGALAAVPFVLKLALERINRPESERGSQARLRSIRDAGYTSETDIF"
BASE COUNT         3838 a         5343 c         5423 g         3934 t
ORIGIN      
        1 ttgccagagg gcgagtggct cgagcgcgtc gcccaaccgc tgcggcaaga tcgacaattg
       61 ctcgcgcaac taacggagag attctggccc gggccgttca cggttgtttt gccaaagcgt
      121 caagtaatcc cggatcttgt cacggctggg ctgaacaccg tcgcagtccg gatcagcgcg
      181 catccgatct ttgccgaggt tgtgcgcacg tttggaaagc cgctcgccgc gccaagcgcc
      241 aaccggttcg gccgcctcag cccgaccacc gcgcagcacg tgcacgatga gctcaacggc
      301 cgcattccac tgatcatcga cggcggcccc acggagcacg gaatcgaatc gaccattgtc
      361 atagttcgcg acggaaaaat cgacatcttg cggcgcggat cgatctcata cgagcagctt
      421 gccgagttcg ccgaggtgaa cgtggtcagc acgacgggca aaatctctgt gccaggacag
      481 ctgaaatcac attacgcgcc gaacactccg ctgtgtttgg tggacaacgc ggcaaacttc
      541 gcgtctgaaa atctgcgtgt cggtttactc gcgtggaatc cagtgacgga tcagaaccga
      601 ttcgctgcga ctcgacttct gagtgagcga cgagattacc gcgaggcggc ggcgaatttg
      661 tttcgctact tgcgcgagct cgacgcgctt gatctcgatc ttatcgttgc agaacgagtt
      721 ccggctcaga ggatcggggc cgcgattctc gatcgactgg aacgcgcggc gcacgattca
      781 ggtcaaacgc gagacaggct tgaatgaacc ctttgaagag cggatgtggt ttgttgggtt
      841 tgctctggaa ttcggggtga tactgacagc caacgaaata cgggtggtca cggagctcga
      901 tcagctcggc cagtgtgtca tcgggcgatg tccctgagat cacgaagccc ttttccgtca
      961 tccgatcgcg atatttcatg ttgaactcat agcgatgccg gtggcgttcc atcacttcgc
     1021 gttcgccata gattctttcg gccaaagtgc cgggcacgat tttggtcggc catgttccga
     1081 gccgcatgct gccgcctttg ctgcggattt tttcacgctg ctcctctaac aacgagatca
     1141 ccggctcggg ggtatttgga tcgaactcag tgctgttcgc tttcgcgatt ccgcaaacgt
     1201 ctcgcacgaa ctcaatggtt gccacctgca tgccgagaca cagcccaagg tagggaatct
     1261 ggtgctcgcg cgcgtaacgc gccgctttga ttttaccttc ggcgccgcga tcgccgaacc
     1321 cacccgggat caagactccg gcgacatctt ttaactgagt gccgacgcgg tcctgaagtg
     1381 attccgcatc gaccagtttc agatcaatcc cgcaatcctg gctcgccgcg gcgtgggtga
     1441 gcgattcgta gatgctcttg taagcgtcct gcaggtgaat gtacttgccg acgaccgcga
     1501 tttttacacg ctcgtgcgga ttgatcacgc actcgacaaa cttccgccag ctcgcgagct
     1561 ccggcgctgg agtttgcagc tgcaagagat tacaaacgag ttcgtccaac ccttctgagt
     1621 gcagcatgag cggcacttcg tagatcgtgt gctgaacgtc acgcgcttcg atcaccgcct
     1681 tttccgacac actgcaaaac atcgacaatt tttggcgcag ttcgcgctcg aggggttttt
     1741 ccgtgcggca aatcaacacc tgcggttgca aaccaatctc gcgcaatttc gcgacgcttt
     1801 gctgtgtcgg tttggtcttt agttcggccg cggccttgat gtacgggacc agcgttacgt
     1861 gcatgttgac cacgttctgc gtgccaactt cgaggatgaa ttgccggatc gcttccagga
     1921 acggcagacc ttcaatgtcg ccggtcgtgc cgccgatctc ggtaatgacg acgtcgtact
     1981 tactttcatc cgagagctcg cggatccggt ctttgataac gtcggtgacg tgcgggatca
     2041 cctgcacggt tttgccgagg taatcgccgc gccgctcttt gaggatgacc gactcgtata
     2101 cctggccact ggtcagattg ttgtgacggg aaagaacgca gttggtgaaa cgctcgtaat
     2161 ggcccaaatc gagatcggtc tcagcaccat cgttcaacac gtatacttcg ccgtgctgga
     2221 atgggttcat cgtcccggga tcgacgttca agtagggatc gaacttctgc aggacgacgc
     2281 ggaggccgcg cgactcgagc aacgtgccaa gcgacgccgc cgcgaggccc ttgcctaacg
     2341 aactaaccac gccgccggtg ataaaaatgt atttcatcgc gagacagctg tccgtgctgc
     2401 gcgagcgagt tttcgctcga gcgcttttgc gtcagccggc gtgtcgacac cgggcgaacc
     2461 atgtttcgtg acgagcacat aaacccttac tccattttca agggcgcgca attgctcgag
     2521 cgactcggca cgctcgagcg acgtcggctt ccacttcacg aatcgcaaaa gcgccggacg
     2581 gcgaaaccca taaatccctt ggtgacgcaa gatccgggtt gatttgcccc gcacaaacgg
     2641 aatcggaaag cgggaaaaat acagagcgcg gctggcaaga tcgacaacta ctttgacttg
     2701 gtgcggtgaa acggcttcca ctgggtcctc aaacggatgc gcggcggtaa cgatgtcgat
     2761 cttgcgatca gaccgcagct tttgcacgat ggagtcgata agcttggggt caatcagcgg
     2821 ctcgtcgccc tggatgttaa tgatcagcgc gaactgcctc gcttttgccg cgacttcggc
     2881 gacgcgctcg gtgccgcttt gatgtcgggg tgacgtgaga gcgacctctg cgccccagct
     2941 gaatgccgcc tcggcgatcc gcatgtcgtc cgtcgcaatc ataaccgcgc cgaggttttt
     3001 agcctctttg gcgcgctccc acacgtgacg gagaagcggc ttgtctgcaa taagatgcaa
     3061 tggtttcccc ggaaaacgcg tcgatcgcca gcgcgccgga acgattccaa cggctttact
     3121 cattcgtaag gatatcgtga gggttagact gaattcggcc ggtcaaaaac gtcacggtcg
     3181 acaaacacgc tgcccattcg ccgttagtcg gcatcatttc gaaagctcgc gatcaactcg
     3241 tcaggagtga cggtcgcggt gccgagcttg cccacgacaa cggcactggc gtggttcgcg
     3301 atctccgccg cttccagcgg cgtcgcctca caggtgagag ccagcgtgaa gagcgcgatt
     3361 gccgtatcgc ctgcaccgga aacatcgaac acctggcgtg ctcgcgtcgg gatgtgatgc
     3421 ggcggttgcc ccggctgaaa gagcatcatg ccttgttcgc cgagagtgat gagcacgttt
     3481 tgggcgtccc atcgtcgcag caaaattttc cccacgcgtg caagctcgac atctcgctcg
     3541 agggactcgc tcggatcttg gtacggaatt cctgcagcca gaaaagcttc ggtccggttc
     3601 ggcttcactg ccgtcatacc gcgccacttg agcaaacgcg tcgcgtttgg atcggcggtg
     3661 acgattttgc cctgcgcgcg cgcagcatgc gtgacttgcg tgacaagttc atcgctcagg
     3721 aagcctttgc cgtaatcctc gaagatcacg gcatcgatgt tcgcgagttg ctttgcgaca
     3781 tctgcgacaa ttttcttcag gtgttcacgt gaagggcccg cgatttgctc acggtcgacg
     3841 cgcaccactt gttgatgtcg cgcaataatt cgcgttttca caatggtgcg gaagttcttg
     3901 ttcacagtcg aattcaacgt cttgatcatt tccccggcca gcagttcccg cagtcgatcg
     3961 ccgctgtcat cgtgcccgat cattcccgcg acgctgacgc gcttcgtaaa ttcacgaaga
     4021 ttgcgcgcga cgttagccgc gccgcccggg acaaatgatt cgtcggtaac ttggacgacc
     4081 ggcacgggcg cttccggaga aatccggccg actttgcccc agacgaattc gtccagcatc
     4141 aaatcgccaa tcacgagcag gcgtttcgca ggcgcgcgat ccagaatgtg ctcgagtcgg
     4201 cgcagcttca ttataaaaag agggcgtgat gccgccgatg ctgaagatca atgcgattca
     4261 ctggcgtcga gatccggtgg tggtgatttg agtggagggc aggttgttgt tagactctgc
     4321 cgcaatttaa tcctgaatac gaaaacgaaa tcaacagtcg aagctcgcga tgattccagt
     4381 ctctaaattt ctgaaggtat gggcatttgc caccatgggc tcggcgttgt gcgcgcacgg
     4441 cgcgccgccg ccgcagcata tcgacgtcaa acagttgtcg cgcaaagtcg aagacgtggt
     4501 ggtgccattg cccaacgaag tattcggcgc gctgaataaa ctcggctcgg ttaactggaa
     4561 agagtacgtg cgcacgaata agggtagcaa ttttactgag cgaccgcgca ttgcgctgtt
     4621 gctcgggaca gtcatcgccg acggcttcat cgctgtccaa gccgaggacg ctccggctgt
     4681 gcgcgatatc ggccagcgtg tccaaactct cgcccgcggc atcggggtcg aacgctcgat
     4741 tacaccgcac gccaaagcga tcaccgaagc ggcggacaaa cggaattggg aaggtgtgcg
     4801 ccaggagctg gaccggacgc agaacagcgt gcagcaggca atgaacgaag tgcacgacga
     4861 gaaattgtcc caactcgtca gcctcggcgg gtggttgcgc ggcacgcaag tattaacggc
     4921 ggtcgtcacg aaacattttt cggcggaagg tgccgagctg ctccatcagc cggacctggt
     4981 ggtttatttc cagacgcgct tggaagcgat gccggagttc aatttgccaa tcgtacggga
     5041 gatccagggc gcgctggtgc aggtgaaacc gctgattgac gtcggcaatg cgcgcatctc
     5101 gcccgcgtcg gtgaagaaaa tcaacgagat cacgacccgg ctcgacaaag gcatcgtgac
     5161 gagatgatga agactcgatg acggcgcacg tgaaatttgc tttgcgcggc agtttgttcg
     5221 ccgcggtgct gctcgggatg cacaccgcgt tgtgcggctc ggtcgatgac gcgcagtcgt
     5281 tcgcactgca agcggccgag ccatatgtga aggaaggttt ccaagtgcgc gaagattatt
     5341 ggggcggcga tctcggctcc ggcgaaaaga aggcggtccg gcaacagttg ttcaaaggaa
     5401 atgaatactg gttctggctg ggcacggatg tgggacgggc gcgcatttcc gtgcacattt
     5461 acgactcaga tgggaaattg gcggaagaat cggacgggtg gcaaaagggc catttcgccg
     5521 ccgctcatat tatcccgaag gcgaccggca gctatttcat catcgtcgcg gtggaggaat
     5581 cacccgagga gcgcacccat tgggcgctgg tctacggatt ccgctaacga gtagatgcgc
     5641 agccatggtg cagtgttggc cgaggccgat cttacctcgt ccgctagccg atgactgaaa
     5701 cgcgcacctt gcagtttgaa agcgcgcggg cactgcagtc gctctatgcg aatgatctca
     5761 agctgctgaa aaatcttgag gaatcgctgg gcgtgaaagt cacgacacgc gaaggttggg
     5821 tgaaactcga aggtgaaccg gacaaaatcg acaaagcgca gcaggtgttc gatcaactcg
     5881 aacaagcgcg gcaaaaaggt gtgccgatcc aaaagcacga atttaactac gcattgaact
     5941 cggtcagcga agcgcgggaa gagaatctcg gcgatcttgc cgcgacaaag attctgactt
     6001 cgccgcgcaa actcccgatc atggcgcgca gtgcaggaca acgcgattac gtcgaggcaa
     6061 tccagaaaca cgacatcgtt ttcggcatcg gaccggcggg gacaggcaag acttatctgg
     6121 ccgttgccat ggctgtcgca gccttgaaaa aggaacaggt cgcgcgaatt attttgacgc
     6181 gccctgcggt cgaggcgggc gaagcactcg gatttttgcc gggtgaactg gaagagaaga
     6241 tcatgccgta tctgcgaccg ctctatgacg cattgcgcga catgcttgaa accgaggaag
     6301 tggagcggct cgttgcgcgt cagaccatcg agatcgcgcc gttggcttat atgcgcggcc
     6361 gtacgctgaa caacgccttc gtcattctcg acgaagcgca gaacacgacg acggagcaga
     6421 tgtttatgct gttgacgcgc atcggtccaa actcaaaatg cgtcatcacg ggcgacatca
     6481 cgcagatcga tctgccgacg aacaagcact caggtgtgct cgaagcatta caagccctga
     6541 aatacgtgcc gggcatcgcg atcattcact tcagcgagcg cgacgtggta cgccacgagc
     6601 ttgtccgtgc catcatcaac gcgtatcaac aacaccgtgc tccgctggat tctgcgcagg
     6661 actagcatgt tcgatttcgt taaacgcaat cggctcgtaa ggcgtggcct cgcttcgcgc
     6721 aagatgcgcc gccggcgccc gcgccatgag tgggccgcga cacttgaatc gtcgggcctg
     6781 gttaagatgt cgatcttcgc ggcgttcatc accggcctcg ccttcctcat cttctccggg
     6841 caacagccgg agccgacgaa aaacttcgtg atcgcgattt tgtttctggc catcgcgatc
     6901 acgcagcttt ggatcaacca gccgaacacg ttcgcgcaaa actcgcgcgt gttgctcgtg
     6961 ttcggaacga tcttcgtgca gctcgcgctg accaagctcc tctacgtgct ctgtgccaag
     7021 cagacgttcc agtttctcac gccagaggtg gcgggactaa tcgcgcccta cgcgttggcg
     7081 ccgctggcgc tcagtgtttt gctcggacgc aaccacggac tttatgcggc ggtttcagtg
     7141 agtctttgga gcagcgtatt gttccgcaaa atcgatgcgc cgcttctcgt caccagcttg
     7201 atcagcggtt tcaccgccgt ttatctcacg ttgcaagtgc ggcgacgcag tcgtctcgtc
     7261 cgcgccggtg tcggcgtggg tgtggcgatc tggttgctct cgctcacgtt tggtttaatc
     7321 gggccgatca atgtgttctc accgatggcg aacgactggg gcatgattgg ggtgcagagc
     7381 gcgctcgcga tcggcaacgg aattgtcacc gcaatgattg tcggcggcgc attgcccatg
     7441 cttgagcatc tgtttcaagt cacgaccgac atttcgtggc tggaagcgtc cgatcttaat
     7501 caccccttgc tacggcggat gacaatcgaa gcaccgggca cctatcatca cagtctcgtc
     7561 gtggcaaatc tcgcggaggc tgccgccgaa gcgatcggcg cgaacgcgac gctctgccgc
     7621 gtgtgctcct attttcacga cgttggcaag ctagtgaagc cgcaatactt caccgaaaac
     7681 atgaacttcg agcgcaaccc gcacgatgat ctcgcgccca cgatgagcgc gctgattatc
     7741 ctttcgcacg ttaaggaagg tgtcgatctt gcgctgaaac ataaactcac ccagcgtatc
     7801 atcgacataa tccaggagca ccacggcacc tcgctcatct cctatttcta tcagcgcggc
     7861 ttgcaacagc aggcggacgc gcgcgccggt ggcaagatca tgaacattcg cgcggaagat
     7921 attcccgaag tgcgcgagga aagcttccgt tatcccggcc caaaaccgca gacgaaagaa
     7981 agcgccatcg tcagtctcgc cgacatcgtc gaaagcgcct cgcgcagttt ggaaaaaccg
     8041 accccgcaaa aaatcgagca actcgtccac gagttgatcg aggaacggat cgccgatggc
     8101 cagctcgacg aatgcgattt aacgctcggg gatttgagaa ccatcagcga ccgcttccgc
     8161 ttcacgctga tgaccatgct gcatacgcgc atcgcgtatc caaaacagga gccgcggatc
     8221 gccggaccgc gcgacgaaac gctccgcccc gatgtcatgg ccgccacgcg ccgcccgccc
     8281 agcgccccgc cggtttctgc agcgtgacct taggctgccg gcggttcgac gtctttgtca
     8341 tgtcgagcga agtcgagaca tctcttgcta ttgatttagc atcgagtcga cagagaaggg
     8401 agatgttatc tcctgaaccc tacttccaga ttcgtaagat cccgtgctcg tccggcggac
     8461 aggacttaaa ggttcggcgg tctggcgcgg ggttcgcgat ctttggcggt ggcggtgctg
     8521 gggcgggtga cgaaggggcc gccttcgagc ggggcggatt tggtgaaggc gacgtcgggc
     8581 atttcgctgg gcagaccttc gagcgggaat tctttacgca gcgggaagaa cggatagccg
     8641 tcccacatca ggatgcggcg gagatcgggg tgcccgttga atttgactcc catcatgtcg
     8701 taaatctcgc gctcgtgcca gttggccgtc ggccagatgt cgctcacggt gtcgaccgcg
     8761 ccgacatctt ccgagacgcg cagtttcagg cgcagatgcg cggccagcgt cattgagtaa
     8821 agctcgtaaa cgatttccca ccgcgggtct tcgccgaagt tgtcgatgct ggtgatgtcg
     8881 atcaaataat cgaacgagag atcgtcccgg cacgatttcg caatctcgcg caggtcttcc
     8941 ggcgcgatcg tgtaggtggt ctctccgcgg aattcactct gatctttgat tttcgcgcca
     9001 aggaatttgc ctaacgaagc gagaagctct tggccggtca cgggaagaaa ggatgaagga
     9061 ttaaggataa aggacgaaag ttgtagggca ggcgctccgc ctgccagtaa tcaaacttca
     9121 cggcaagcga tgcgcttgcc ctacaatttc tgttggaagc gttcacgcca gcgcttcctc
     9181 agcttttttc gtaagcgcag gtttctgcgc acgcagcgaa cgctcgccgg aaattttctc
     9241 ctgcaatttc atcagcccgg cgagcaacgc ttcgggacgc ggggggcagc cggagatgta
     9301 aacatcgacc ggcaggagct gatcgattcc ctgaagcaca gcgtaggagc gatacattcc
     9361 gccggtggat gcgcatgcgc ccatggcgat gacccatttc ggctcgggca tctgctcata
     9421 aatgcgtttc accgccagcg ccattttgta tgtgacagtc ccggcgacga tcatcacgtc
     9481 gcattggcgc ggcgagaaac gcatgacttc agcgccgaag cgcgagatgt cgaagcgcga
     9541 ggcggcggtc gccatcaact cgatcgcgca gcaggcgagc cccattggca tcggccagag
     9601 cgaatttttg cgcacccaat tcaacgccgc gtcgaagcgg gtgaaaatga cgttgccttc
     9661 gatctttgaa tcgtaggcgg ttgaacgttc ttcggccatc gcgcaacagt aacgtcgcac
     9721 cccacgcatt caagatcgac aatcggtgga ttggccgctc ggcgcgtgtt gctaaatgat
     9781 tgcggctgcg ccgcgagtca acatcgaacg ccggagcacc cgatccacct ttgcagcgtt
     9841 accttgtcga ttgtatagaa cgactcccga aaaccgcgat gaactcggcg atacgcactc
     9901 atctcgacga gctagagaag caaagcattt tcatctttca cgaggcgtat cgcacgttca
     9961 agaacatcgc catgccatcg tcgataggaa aagattcgaa cgtgttgatc tggctggcgc
    10021 gcaaagcgtt cttcgggggc gtcccgttcc ccgtgcagca tatcgacacg acttagcacg
    10081 atgagagtga gacacgttga aggactaagt gggttttgag cggcagcaaa tttcgacaag
    10141 tgccgagctc gactgggagc agcgacgcat tatgactatt gccaaaaact ccggtttgtg
    10201 ggaggaattg aactgcttct tgcctgaggc ggcgccgcat tttcgcacga aaaccggcaa
    10261 aacgacccaa ccaagcaaga aacccttatc attatgaata ttgatagcgc aagatttcag
    10321 ggccatctca ttgcgccgca tggcggcgaa ctggtcgatc ttaaactcga tccggaaaat
    10381 gcagccgaga tcaaagcgcg ctccagagat tttccgtcct gggacttaac gagccggcaa
    10441 atccgcgacc tcgagcttct gctgagcggc ggcttctcac cgctacgtgg cttcatgaat
    10501 cgcgacgatt acgagcgcgt gtgccaggag atgaaattga gcaatgacct gctctggcca
    10561 atcccggtca cgctcgatgt gccggaggcg tttgccaaat cgctcaagcc cggagccagc
    10621 aaaatcgcgt tgcgtgattc cgaaggcgta atggtagcgg tcttgtatgt cgaggatgtc
    10681 tggcagcctg atcgaaaggc cgaagcgcaa gctgttctgg gaacgacgag cacagtacat
    10741 cctggtgtcg attatcttct caaccgggga cacggctggt acgtgggcgg gcgcgtggaa
    10801 ggtttgcaac tgcccaccca ttacgatttc cgaacccttc ggctcacacc gtcagatttg
    10861 cgggcggagt tcgtgcgccg cggctggcgc cggatcgtcg cgttccagac gcgcaacccg
    10921 atgcaccgcg cgcacgtgga attgacgttt cgcgccgcga gacaggtcga agccagtctt
    10981 ctgattcatc cttccgtcgg cgtgacccgg cctggcgacg tggactactt cacgcgcgtc
    11041 cgttgttacc agctgcttgt ctccaaatat ccgcgcggca cagccaagct tgccttattg
    11101 ccgcttgcga tgcggatggg cggaccgcgg gaagcgatct ggcacgcgat cattcggaaa
    11161 aaccatggct gctcgcattt tattgtcgga cgcgatcacg ccgggccggg caacgatgcc
    11221 gatggcaagc cattctacgg gccatacgaa gcgcaggagt tgttccgcaa acaccaagcc
    11281 gacatcggtg ttgaaatggt gcctttccaa atgatggtct atgtcgagga ccgagataaa
    11341 tatttcccag aaaacgaagt gccgcccggg gcgcgcgtgc tcaatctctc gggcacagaa
    11401 ttgcggcgcc ggctaaacga aggccgcgac attccggtct ggttcacgtt tcccgaaata
    11461 gcgcgcgaac tgcgacgcag cttcccacca cggcataagc aaggtctgac cgtcttcttt
    11521 agcggactct ccggcgccgg gaaatcgacc atcgccaacg tgctcatgat taagttcctc
    11581 gaaatgggcg gccggccagt gacactgctc gacggggatc tggtgcgcaa acatctctct
    11641 tcggagcttg gtttttcgaa ggaacatcgc gacatcaaca ttcgccgcat tggctacgtg
    11701 gcctccgaga tcacgaaaaa cggcggtatt gctatctgcg cgccgatcgc gccgtatgac
    11761 tccacgcgca aggacgtccg ggcactgatt gagccatttg gcggctttgt tcttgtgcac
    11821 cttgccacgc cgatagaagt ttgcgagcag cgtgaccgaa agggcctcta cgcgaaagct
    11881 cgtgctgggg tcttgaagga atttaccgga atatcggatc cctacgaagt gccgactgac
    11941 gcggaagtga cggtggacac agcagagctg tcgccggagg aagccgccca ggaaatcatt
    12001 ttgcatttgg aacaggaagg cttcatcgga atcaacggca cggcgcctga tccaaactcc
    12061 acgtagccgc aggcgccccg caccggatcg aaggtaggtc gcgttctccg gacgcgacgt
    12121 ttagttgtcc cgcggacacg agaggcttta ttagcaacgc gcccggaggt cgcgttccac
    12181 cttgcctaac gagcgagttc tcgatcttac gattcgtgcc gcgcgcacga gcgacgcagc
    12241 cgcgctcgcg cggctgatgt gtgagctcgg ctacgagacc accacggcgg agatggcatc
    12301 tcgattagaa accatcactg gcgatccgcg ctatcgaaca ttggtcgcgg tcgcaaaagg
    12361 aaaggtagag ggaatgatcg ggacgtttgc tcgccccagc tttgagcaca acgacctcag
    12421 cggtcgcatt ctcgcgctga tcgtcgcctc aaccgcgcgg cgccgtggca ttggccagcg
    12481 attgattgcg gcggcagaaa acgatttcgc ccgaagaaat attcggcgcg tcgcggtaga
    12541 cacgcgattt gagcgggaac aagcacatga gttttacgaa acgctgggct accgccggaa
    12601 tgggttccgt tttgtgaaaa cgttagatta gtggcggcga ggccgacggc ttgcgcttca
    12661 ccttaggcga cgcgagtggc gaggcggccg ccttcaatgt gctcgccgta gttgacgtgg
    12721 ctcgacgcga tgacgtcgtg gatgattttg cccagctcag ccgcttcgta tggcttcgcg
    12781 ataacgccgc gaaacccgaa atcctgatac cggctcatcg tcgcatcggt ggcgtaaccg
    12841 ctggagacga tcgcattcac ggttggatcg atctcgatga gctttttcaa agcttccttg
    12901 ccgcccatcc cgcccggcag cgtgaggtcg agaatgacag catcgaagcg ttcaccgcgc
    12961 tcaaatcttt ctcggtaaat gttgacgccg tcgagtgcgg tgtcggcctg cttcacttcg
    13021 taaccgagtc gggtgagcgt aaactcgacc agcgtgcgaa tggcttcctc atcgtcgaca
    13081 accagcacac gaccggtgcc agtcattgcg ggtgacatgg tcactggagc gtcaatcggc
    13141 agttcctgat ggagcgcggc cgggagatag accgtaaacg tagaaccgaa gtgcacctgc
    13201 gattcgacgg tgataagacc gttgtggttt ttgatgatcg agtaagcggt ggccagcccg
    13261 aggccgttcc ctttaggttt cgtggtgaaa taaggatcga agatgcgttt gacgtattgc
    13321 tcgggaatgc cgacgccttc gtcccgaatg ctgatgcgga tgtaatcgcc tggcggcaga
    13381 tcgggaatca ccggcgtagt tgcgctcgtg tagcggaagt tttcgcaaat cacatgtaac
    13441 gtgccgccgt tcggcatcgc ttgatcggca ttcaccgcga gattcgcgat aacctggcta
    13501 atttgccccg gatcgatctc ggccggccac agatcggccc cgaattggaa ctcgctgcgg
    13561 ctatggctgc cgcgcaacga gaagccgacc gtatcctgaa tgagtttgcc aatcgacgtt
    13621 gttttcttaa tcggcgcacc gccgcgcgcg aaggtgagaa gctgttgcgc gagatcgcgg
    13681 gcgcgtatgg aagcattttt tgcgtcaacc agacgcgtgg ccatctcggc atcgggtgga
    13741 agaagtagtg aagcgagcga gatgttgccg ataatcgcgg tgagcaggtt gttgaagtcg
    13801 tgcgcgatgc cgcctgcgag caagccaagt tgctcgagtg tttccgcttt gcggcgctca
    13861 gcttcgtcgc gctggcgctg ggtgatatcg cggaacacca gaacaacacc ggcgacttca
    13921 tttttgttgt cgcgaatcgg cgacgcgctc tgctcgataa tgcgctcggt gccatcgcgc
    13981 gaagtaagcg tcgcgttttc tgctagcccg agcaggatcg attgcacttc gttgcgataa
    14041 gcactgcgcg ggacgcgcgc ctgcgcggca aggtcgatcg ccacattgaa taccgtcttg
    14101 agcggctggc cgatggcttc ctgtgatgtc cacccggtca aacgttcagc ttcgttattc
    14161 aaaatgatga tgcggccctg cacgtccgtc gtaatgacac cgtcaccgat ggaacggagc
    14221 gtcacggcca ggctttcttt ttccgccgcg atttccttct cgaatcgccg gcgctcggtt
    14281 acatcgcgca ccatcgccac gaacagtttg ttgtcgccca cctgcatctg gctgagcgac
    14341 atctcgagcg ggaagctgac gagctttcgg gttcgtccca gcgcgagaat ggtgctgccg
    14401 gtgcgcttcg cgatttgttc ccacgcacag gcgaccggtt tggcgtctgg ctcatctccg
    14461 taaaccttcg gcacgagttt ggtcaatttg tagccgacca tttcctgctc gctgtagccg
    14521 aacatttttt cggccgccgg gttcatcgag caaatcaccc cttcttcgtc gagcgtaacc
    14581 atgccgtcga gaatgttttc gataatcgag cgaatgcgtg tttcgacggc ttcgagtttg
    14641 gcgagctgcc ttcgcacgag agcgaagctg tagctgtttg ataccaccag taatgcgacg
    14701 gcaagaaagg cgaggcatcc gaggatggat gttttgatga cgcgctcgcg gctcgccgcg
    14761 gagtttcgca tctcgtagac cgacacctcg ttttgctcga gcgtgccgag catctggcgg
    14821 atatcggcca tcaggggttc gccaacgtcg ctaatcatga gcgtgttgat ttctttgccg
    14881 tcacgccggg cgtcgatttc cggtgtcgcg caggtgctaa cccaccgctc gacgttcgcg
    14941 cgcacctctg ccagcaactc ggcctgactg ggactgttcg ccaccaagat cgacaggtag
    15001 ccgttgtatg tataaaagtc gctgactgcg cgtttgtaat tttcgaggaa actggcctcg
    15061 ccggtgagaa gatagccgcg cttctccttc tgcatctcca ataccgaccg ctccagtttc
    15121 gggaggaaat caagaatctg cgtggattgc gtcgcccatt cctgttcaag catgcgctgg
    15181 ttgagcacaa tctgctcgtc gtcctggacg gactggagaa tggcgcgtgc ctgatccagg
    15241 agtgagttgc cgagggcgac tggttgagaa tcggggctgc tcgtgtccgg gctgccagac
    15301 gtggcctgcc gcgctttgat ctccggcacg gcgaccgtat caatccattg ctgcacgatc
    15361 gtctgcacct tgacgatgcg tttacgttgc ttcgggttgt caaaaatcaa cgcgttcagg
    15421 ttttcgatcc gccgcttgat cgcttcgcga cgccggtcaa atgacaacgt gtaatcgttt
    15481 ttgccggtca gcagaaagcc gcggtgaccg gtttcgagcg cggcgatatc attttcaatc
    15541 aaactgctca ggcggatcac ttcgatggtg tgcgcacgca cattttcctg ctggtgattg
    15601 cggattgtga cgtaaaggaa aatcccagcc agcagcacga ttaccgtcac cccggcggcg
    15661 gtgccggaga gaagcttgaa atgaaaatgg cgctggctaa aaagccattc tccgcatcgg
    15721 cgaaagaagc cgacgcgttt cggtttatcg gtcgtggtgc tgactggcga tggctcggcg
    15781 tggcgcatct ataattttta taaaagcagg tattctgcca ctccgttcct ctgccgctat
    15841 cagcgcgccg tcgtctgcta aagcaatctc agtaccggac gcggacgaga aataatccgg
    15901 cagcgggtgc aacaaaccgg cggccggcac tctggcggcg gctcaactga tttgcgattt
    15961 ccgcgactgt ttgtttgccg agcgcgcatt gcacgatcga accgaccatt agtcgcacca
    16021 ttttgtagag gaaaccgtcg ccgtcgaact cgatgttaac gcgcgaaccg cggtgcagac
    16081 ttcgcaccga acgaatcgtt cgagtggtgt ttgcttcagt atgaccgcga tttgcagcga
    16141 acgcggcaaa atcatgcatg ccgacgaact cctgcgcaga cctctccagc ctgtcgaagt
    16201 cgagcttcgc cgccaaatgc catgctcgtc cgtattcatg tggtggcatc accggcccgg
    16261 accaaatgcg gtaacgataa actttcccgt tcgcagaaag ccgcgcgtga aaatgggttg
    16321 agacaaagga gcatcgcaac acgcgaatag tcggttgcag gacggcattt aacgcctgca
    16381 cccagtcgcc agcggccaac ttcggttccg caagatcgac atgtgcgcat tgaccgagcg
    16441 cgtgaacgcc agtatcggtc cgacccgcgc cctgtacacg cacgcgctgg ccactgatac
    16501 gatcaaacgc gcgctccagc tggtcctgga tcgtgtttcg gtgtgtctga ctctgccagc
    16561 cggcgaaatg catgccgtca taggcgagga tcaacttcag tcgctgcgac atcgcgttac
    16621 cgcgcaatct ctttcacatc gctgattgcc tgcgcgagtc gagatagccc tgcaagatca
    16681 tctgcgctgc gacttgatcg agatactcgc ggctccggcg cgtgcttttg cctgcgctcc
    16741 gcagcgcgcg gttcgcggct gcggtcgtga gccgctcgtc ccatgtcacc acctcacacg
    16801 gcagcatcgc gcgcaacttg tccgcgaaac ccaaagctga tttggccgcg tctccaactt
    16861 caccgctcat gcggcgcggc aagccgacca ccacgctgtc gatcttgcgt tgccgaacaa
    16921 tttcggcgat gcgtgctggc gcgtttcccg tcgcaggaat tgtctcgagt ggatgcgcga
    16981 gcaattggag ctcatccgaa atcgccaaac cgatgcggac ttttccgaga tcgatcccaa
    17041 gaatcgggtt catttggccg cgggcggttc actgcagctc gggcggaagt ttggtgacca
    17101 ggttcttgat cttctccacg tcgtggcgat gacagaccag gacggcatcg cctgtgcgta
    17161 ccacgatgaa atcgtgcaca ccgagcaatg cgacagtcgt ctcgtcttcc gcgaaaataa
    17221 tgttgtgagt cgactcaagc gcgacaagcg ggcaattcgc cgcgttgccg tcgatgtcgc
    17281 gcttgaagta attggcgact gcctgccaac tgccgatgtc gtcccaatca aagctcgcct
    17341 cgacgaccag gacgcgatcg gccttctcca tgattgcgta gtcgaaggaa atccgcggga
    17401 gcttggcgaa acgttcgccc agcacttttt cgaatcttcc gggcgcgcgc agctgcgaaa
    17461 tgaagtcggc cagctcgggc gcgtggcggt taaactcgct cagcacggtc ggcaccgacc
    17521 agacgaacat cccggcgttc caacgaaaat tgcccttgcg caagaatgat tccgcaagat
    17581 cgacattggg cttttcgcga aagcggacga ccctgtgcac cgcgcctttg tcggcgccgt
    17641 tttggaatcg aaccgcttcg ccatgctcga tataaccata gcccggacaa ggccacgtcg
    17701 gcttgacacc gatcacgacc agttcgccca tttgcgcggc ggcctcagcc gctaagctca
    17761 atgtctcgtg aaacgccgcg cgattcgcaa tcaggtggtc ggcgggaagg acgagcatgg
    17821 tcgcgccgtg atcgcgcgcc gcgacccagc cggccccgag cgcaaccgcc gcggcggtat
    17881 cgcgttttgc tggctcggcc aaaatatttt ctttcgggaa atcgctgagc aattcgcgca
    17941 cagcatctgc ttgttcgaca ttcgtgagaa cgaggatgcg ctcccgtgga acgaggccgt
    18001 cgagccgcgc aaccgtttcc tcgagcagcg cgcgcttcgt caccaattgg agcaactgct
    18061 ttggacgtgc gcggcgactg agtggccaga atcgttggcc actcccgcca gcgagaatga
    18121 gcgcgtaaat cagattcgat attgccatgc aaaaatctgc gccggctggc tatccgcagc
    18181 gcgaagattc ccgtttcctt ttaccaaacc ggacgtataa gggtatgcga tgtcgctccc
    18241 gttatccatg ttaaaaagct ccgaccagct gccactcgat gcgttactta ccctgacgcg
    18301 taccgcgttc ggccttggaa ttgggatgct catggccgac aagatcaagc gccccgttcg
    18361 acaagcggcc ggcatcacat tgctctcgat cggcgcgctc gctgctgttc cgtttgtgct
    18421 caagctcgcg ctggaacgaa ttaaccggcc ggaatccgag cgcggcagtc aggcgcggct
    18481 gcgatcgatt cgagacgcgg gttacacctc cgagaccgac atcttctaag cggctcgc
//
LOCUS       DASTDG010000092         6408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1562035, whole genome shotgun sequence.
ACCESSION   DASTDG010000092 DASTDG010000000
VERSION     DASTDG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562035"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFO30_02915"
     CDS             complement(<1..345)
                     /locus_tag="VFO30_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418266.1"
                     /translation="MAKPKPLRIANVSIGGKRPAFILGPCVIESEKFVWRMARQIAEI
                     CATEDVDLIFKASYDKANRTSVRAFRGIGVREGCEILSAIGQELKIPVTTDVHSPGEA
                     EIAGEKIDMLQIP"
     gene            complement(377..1117)
                     /locus_tag="VFO30_02920"
     CDS             complement(377..1117)
                     /locus_tag="VFO30_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET9418267.1"
                     /translation="MDSAPEFYPYRLPHLTVICLTIALPFALAALVRSSKSHRVERMI
                     VSTISVILLLDYVTYLALIRRTAVVAWQQLLPLQLCDWGMVVVIVAMWTGRQRWFEVA
                     YFWGIGGTVQAVLTPNLHYGFPDFRFFSFFISHCGIIVGVVFLMLTRHYRPLPMSIVR
                     VFLWSELYFLVTFIADAVTGYNYGFLLHKPEAFSILNFLSDSRPLYLLQMHGLALLFF
                     IVLYAPFAIVDLARGKSPRNAGKQEASI"
     gene            complement(1145..1963)
                     /locus_tag="VFO30_02925"
     CDS             complement(1145..1963)
                     /locus_tag="VFO30_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9418268.1"
                     /translation="MAAAPPYKNLAVASTFSPRFEAVLAEAKRVRDRLGSTLSLIHVG
                     QRSEESARKFSDAFARLELPTGSTIHYREGDPAEAILEFLAHHPIDLVLAGALEKELV
                     LHPFLGNVARRLVREAACSVFLFTNPLREPKPLRRIVFLITDSEDAQRAFAHTLQLAR
                     AEQCERLYVIRVVTTFDQARAAMPAAKNGRRSRSFEDEEVALEQFVLKAGETDVPIEV
                     RCLRANTGFAAADFVQSTAADLLVVPVRTAPGRELPKNIAWLTDIIPCNLWLIR"
     gene            2083..2562
                     /gene="coaD"
                     /locus_tag="VFO30_02930"
     CDS             2083..2562
                     /gene="coaD"
                     /locus_tag="VFO30_02930"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9418269.1"
                     /translation="MGRAIYPGSFDPVTNGHLDVIERGRKLFDEVVVAVAYNEEKNAL
                     FSLEERLQLLEQSIGQINNVRIAQFDGLLVDFAVAQQANAVIRGLRAVSDFEFEFQMA
                     LMNRKLETAVETIFLMPKEEYTYLSSRIIKEIARLGGDVSSFVPPAVVKALRRKFGK"
     gene            2559..3002
                     /locus_tag="VFO30_02935"
     CDS             2559..3002
                     /locus_tag="VFO30_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418270.1"
                     /translation="MKVHLKQVPADGLHLEGEEDCPITEFEAEEIRGVGPLSYNIDIG
                     VAGGALWATGSLRQKAELRCVACLEKFVYELSVPAFAMRTELHGPETVDLTPFMREDL
                     LLNLPAHPRCDRDGGRVCKAAQSQPATQNAKGRLDWSELDKLRLK"
     gene            3112..3294
                     /gene="rpmF"
                     /locus_tag="VFO30_02940"
     CDS             3112..3294
                     /gene="rpmF"
                     /locus_tag="VFO30_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9418271.1"
                     /translation="MGVPKRKTSKMRLRSRKAANRWHAPRLSKCPQCGSTVPGHTVCP
                     SCGYYRGRQVLTLEGK"
     gene            3432..4463
                     /gene="plsX"
                     /locus_tag="VFO30_02945"
     CDS             3432..4463
                     /gene="plsX"
                     /locus_tag="VFO30_02945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9418272.1"
                     /translation="MKIALDAMGGDFGPPNLVAGAVMALQEYRRIDRLFLVGDAPKIE
                     TELRKHGCSDQRVEIVHSTQVVDMSDRAWSAVRRKRDSSISRAVDLVKHGQADAAVSA
                     GHTGAAVAASMVKLRTLEGIDRPGIAAVLPTQYNVFVLIDAGANIDARPEHLLQYAIM
                     GSVYSSHVLGYKRPSVGLISLGEEDVKGNELTKEAFKMVKQSSLNFRGNIEGRHLFEE
                     PVEVVVCDGFVGNVILKTSESIAVAIFKWLKDELMQSRRRMAGAYLAKDAFHNIKKKT
                     NYEEYGGMPLLGVNGICIIAHGASTPLAIKNALRVAAESIEQQVNPHIVEEIRRYNEA
                     TAPLEAAVH"
     gene            4426..5445
                     /locus_tag="VFO30_02950"
     CDS             4426..5445
                     /locus_tag="VFO30_02950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9418273.1"
                     /translation="MKRQPRSRPRSTKRRSVSIIGTGSYVPEKILTNDDLTRIVDTSD
                     EWITTRTGIKERRVAAKDEHTSDMATKAALQAIDQAKISPTDINLILVATATPDMLFP
                     ATACFVQKKIGATNAACLDVSAACAGFLFALEIGQQFITSHTYDIVLVIGADKLTSVT
                     NWTDRNTCVLFGDGAGAAILAHRGGSHGVISTHIGSDGRFSDILFMPGGGSRCPITRE
                     NAHLNLATIHMTGKEVYKQAVTAMLSAAKKALDDAGLSIQDIACVIPHQANLRIIEAI
                     ADRLGIPLEKFMVNLDRYGNTSAAAVAIALDEANRSGRIKTGDYVLMVVFGGGLTWAG
                     TVLEW"
     gene            5527..6393
                     /locus_tag="VFO30_02955"
     CDS             5527..6393
                     /locus_tag="VFO30_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9418274.1"
                     /translation="MLIETHAHLDYPDFADDFEDVLRRATEAGVTRIITIGTSVESSR
                     RAVDLAEKYPNIFAVIGVHPTYAAEAEDDVMTPLRELAKNPRVVGLGEAGLDYHHLPS
                     VEAAKEKKVQVFARALQGETEEEIEASIQDGAYKSRQASLFQQQLDLAVELGFNVVIH
                     QRDAWADTLAIMQPYTDKLRGVFHCFGGTLEQANEVLALDHLVSFTGIVTFKNGAAVR
                     EVAARVPLWKFMVETDCPYLAPVPFRGKRCEPAHTRLVAEAIAAARGVSLQEVAEATT
                     ETAEKFFRFNRA"
BASE COUNT         1439 a         1722 c         1963 g         1284 t
ORIGIN      
        1 cgggatctgc aacatgtcga tcttctctcc ggcaatctcc gcttcccccg gtgaatggac
       61 gtccgtcgtg accggaattt tcaactcctg gccgatcgct gaaagaatct cacagccttc
      121 gcgcacgcca attccgcgaa acgcgcgcac ggaagtccgg ttcgccttat cgtaagatgc
      181 cttaaaaata agatcgacat cttcggtcgc acaaatctcc gcaatctgtc gcgccatgcg
      241 ccagacaaac ttctccgatt caatcacgca cgggccgaga atgaacgcgg ggcgcttccc
      301 gccaatcgaa acatttgcga tgcggagagg tttcggtttc gccatgagat ctaaggaaag
      361 caggaacgca ggaattttaa atgcttgctt cctgctttcc tgcattccta ggggattttc
      421 ctctggcaag atcgacgatc gcaaatggcg catagagcac gatgaagaag agcaacgcga
      481 ggccgtgcat ttgcagcagg tacagcgggc gcgagtcgga caggaaattt agaatcgaaa
      541 acgcctccgg tttgtgcaga agaaacccgt aattgtagcc ggtcaccgcg tcggcgatga
      601 aggtaaccag gaaatacaat tccgaccaga ggaacacgcg cacgatcgac atcggtagag
      661 gacgatagtg gcgggtgagc atgagaaata cgacgccgac aattatcccg cagtgcgaaa
      721 tgaaaaagct gaagaagcga aagtcgggga agccatagtg tagattgggc gtgagcacgg
      781 cctgcacggt gccgccgatt ccccaaaaat acgcaacctc aaaccagcgc tgcctgccgg
      841 tccacatcgc aacgatgacg acgaccatgc cccagtcgca gagttgcagg ggaagcagtt
      901 gctgccatgc aaccactgcg gtgcgccgaa ttaaggcgag gtaggtgacg taatcgagaa
      961 gcaggatcac ggaaattgta ctgacgatca tgcgctccac ccgatgcgac ttgctgctcc
     1021 gcacgagcgc ggccagggca aacggcagcg caatcgtcag acaaatgacc gtgagatgcg
     1081 gaagacgata tggataaaac tcaggcgcgg aatccatcgc aagatcgata gtgtgcgcca
     1141 ctcatcagcg gatgagccag agattgcacg gaattatgtc agtgagccag gcgatgttct
     1201 tcggaagctc acgcccgggt gcagtcctca ccggaacaac gagcagatcg gcagccgttg
     1261 attggacgaa atcagccgcg gcgaagccgg tgttggctcg caaacagcgc acttcgatcg
     1321 gcacgtccgt ttcacccgct tttaacacga attgttcgag ggcgacttct tcgtcctcaa
     1381 aactgcgcga acgccgcccg ttcttcgcgg ccggcatggc ggctcgcgct tggtcgaaag
     1441 ttgtgaccac acgaatgacg taaagccgct cgcactgttc ggcgcgggcg agttgaagcg
     1501 tatgcgcaaa tgcacgctgc gcatcttcgg aatcagtgat cagaaaaacg atgcgccgca
     1561 gtggcttcgg ctcgcggagc gggtttgtga aaaggaaaac tgaacaagcg gcctcgcgta
     1621 ccagccgccg ggcgacgttg ccgaggaagg ggtgcagcac gagctctttt tccaatgcac
     1681 cggcgagaac aagatcgatt ggatgatggg cgaggaactc taagatcgcc tcggccgggt
     1741 cgccttcgcg gtagtgaatg gtcgagccgg tcggcagctc taatcgagcg aacgcgtcgc
     1801 tgaatttccg tgcgctctcc tcgctccgct ggccgacatg gatcaggctc aaggtcgagc
     1861 cgagccggtc gcgcacccgt ttggcttcag cgagcacggc ttcgaagcgc ggcgaaaatg
     1921 tgctcgcgac ggcgaggttt ttgtaaggcg gagcggctgc cattgaaggg gcgatcaatc
     1981 tccggcgagc ggcggcttac gcaacgaggg aaatgttcgc cgcaggacga acccgtccgg
     2041 tgcggatttg atgtttgatc tttcaggttt gatgttccgc cgatggggcg ggcgatttac
     2101 ccggggagct ttgatccggt gacgaatggc catctcgatg tgatcgagcg cgggcgaaag
     2161 cttttcgacg aagtagtcgt ggccgtcgcg tacaatgagg agaaaaacgc gcttttctcg
     2221 ctcgaagaac gcttgcagct gctcgagcaa tcgattggcc agatcaacaa tgtgcggatc
     2281 gcgcaatttg atggattgct cgtcgacttt gccgtcgcgc aacaggcgaa cgcggtcatt
     2341 cgcggcctcc gcgccgtgtc tgatttcgaa ttcgaatttc aaatggcgtt gatgaatcga
     2401 aaactggaga cggcggtgga aacgattttt ttaatgccga aggaggaata cacctacttg
     2461 agctcgcgga ttatcaagga gatcgcgcgc ttgggcggtg acgtatcaag tttcgtcccg
     2521 ccggcagtgg taaaggcttt gcgccggaaa tttggcaaat gaaggttcat ctgaaacaag
     2581 ttccagcgga cgggctgcat ctcgaaggcg aagaggattg cccaatcacc gagtttgaag
     2641 cggaggaaat tcgcggcgtc ggtccgcttt catacaacat cgacatcggc gtggccggtg
     2701 gagcgttgtg ggcgaccggt tcgcttcggc agaaagcgga attgcgctgc gtggcctgtc
     2761 tggaaaaatt cgtgtatgaa ctgagcgtgc cggccttcgc gatgcgcacc gaattgcatg
     2821 gtccggaaac tgtggatctt acgccgttca tgcgagagga tctgttgctg aatctgccgg
     2881 cgcatccgcg ttgcgatcgg gacggcggac gcgtttgcaa agcggcgcag tcgcaaccgg
     2941 ccactcagaa cgcgaaagga aggttggact ggagcgagct tgataagttg aggttgaagt
     3001 gatgaagcgt taaacagttg aatcggataa ctcagaccgc tgtaattctt gatcgccgta
     3061 acgcttcaac cattgttttt ctgtggggat atgcttcggt gaacgccggc catgggtgtc
     3121 ccaaaacgta aaacgtcgaa gatgcgattg cgctcgcgga aagcggcgaa ccgttggcac
     3181 gcgccgcgtc tgagcaaatg tccgcagtgc ggcagcacag tgccagggca cacggtttgt
     3241 ccgtcctgcg ggtattatcg cggccgtcag gtgctgacgc ttgaaggcaa ataactccgg
     3301 ttgagcgttg aagcgataaa gcgttgaagc gttaccgcgg cgttgctcgg cttcaactct
     3361 tcaacgcttg aaccttttag cgcatttttc gttttgcatt cacgaggcaa agttcgcaaa
     3421 gtttctgccc gatgaaaatc gccttggatg cgatgggggg cgattttggt cccccgaacc
     3481 tcgtggcggg cgccgtgatg gcgttgcagg aatatcggcg gatcgatcgc ctttttcttg
     3541 tcggtgacgc gccaaaaatc gaaaccgagc ttcgcaagca tgggtgcagc gaccagcggg
     3601 tcgagatcgt gcactcgacc caggtggtcg acatgagcga tcgggcctgg tcggcggtgc
     3661 gacggaaacg agattcctcg atcagccgcg cggtcgatct tgtgaaacac ggccaggcgg
     3721 atgcagccgt cagtgccggt cacactgggg cggcggtggc ggcgagcatg gtcaaattgc
     3781 gcacgctgga agggatcgat cgcccgggga ttgcggcggt tttgccaacg caatacaacg
     3841 tttttgtcct gatcgatgcc ggcgccaaca tcgacgcgcg gccggagcat ttgttgcagt
     3901 acgccatcat gggctcggtt tattcgagtc acgtcctcgg ttacaaacgg ccctcggttg
     3961 ggctcatctc actcggcgag gaagacgtca aaggcaacga gctgacgaaa gaggcgttca
     4021 agatggtgaa gcaaagctcg ctgaattttc gtggcaacat cgaagggcga cacctattcg
     4081 aagagccggt ggaggtcgtc gtgtgcgacg gctttgtcgg caatgtgatc ttgaaaacgt
     4141 ccgaatcgat cgcggttgcg attttcaaat ggttgaaaga cgaactgatg caatcgcgcc
     4201 ggcgcatggc cggcgcctat ctcgccaagg acgcgtttca caacatcaag aaaaagacca
     4261 attacgagga atacggcggg atgccgttgc tgggcgtaaa tgggatttgc atcatcgcgc
     4321 acggggcgag cacgccgctc gccatcaaga acgcgttgcg ggtggcggcc gagtcgattg
     4381 aacagcaggt gaacccgcat atcgtcgagg agatccgccg ttacaatgaa gcgacagccc
     4441 cgctcgaggc cgcggtccac taaacgccgc tccgtttcca tcatcggcac cggcagttac
     4501 gtgccggaga aaatcctcac caacgacgat ctgacgcgca tcgttgatac gtcggacgaa
     4561 tggatcacca cgcgcacagg tatcaaggag cgccgcgtcg cggccaagga cgagcacacg
     4621 agcgatatgg cgacaaaggc agcgctccaa gcaattgacc aggccaagat ctcgcccacg
     4681 gacatcaacc tcattctcgt cgcgacggcc acccccgaca tgctgtttcc ggcgacggcg
     4741 tgctttgtgc aaaagaagat cggcgctacg aatgccgctt gtctcgatgt ttccgcggcg
     4801 tgcgcggggt ttctgtttgc cctcgaaatc ggacagcaat tcatcacttc gcacacttac
     4861 gacatcgtgc tcgtcatcgg cgccgataaa ctgacctcgg tcacgaactg gaccgaccga
     4921 aacacctgtg tgcttttcgg cgatggtgcg ggcgcggcga ttttagcgca ccgtggcggc
     4981 agtcatggcg taatcagcac gcacatcggc agcgacgggc gcttcagcga tatcctgttc
     5041 atgccgggag gcggatcgcg ttgcccgatc acgcgtgaaa acgcgcactt gaatctggcg
     5101 accatccaca tgaccggcaa ggaagtttac aaacaagcgg tcacagcgat gctgagcgcc
     5161 gcgaaaaaag ctctcgatga tgccggcttg tcgatccagg acattgcctg tgtcattccg
     5221 caccaggcaa acttgcgtat catcgaagcg atcgcggatc gtctggggat tccgctcgag
     5281 aaattcatgg tgaatctcga tcgctacgga aatacttcgg cggccgcagt tgcgatcgcg
     5341 ctcgatgaag cgaatcgcag cggacgcatc aaaaccggtg attacgtcct catggtggta
     5401 ttcggtggcg ggctgacctg ggccggcacg gtgctggagt ggtgaagccc gtacgtaatt
     5461 cgtgattggt gaatcgagaa tcggagccga tgtaacgatt cacgattcaa cgatgtaacg
     5521 attctcatgc tcatcgagac tcacgcacac ctcgactacc ccgatttcgc ggatgatttt
     5581 gaggacgtgc tgcgtcgtgc gaccgaagca ggtgtgaccc gtatcatcac cattggcacg
     5641 tcagttgaaa gcagccggcg cgctgtcgat cttgcggaga agtacccgaa tattttcgcg
     5701 gtaatcggcg tgcatccgac ctacgcggcg gaagcggagg atgacgtgat gacgccgttg
     5761 cgcgagctgg ccaaaaatcc gcgggtcgtc ggcctgggcg aagccggcct cgattaccat
     5821 catctgccga gcgtcgaagc ggcgaaggaa aagaaggtgc aagttttcgc gcgcgccttg
     5881 cagggggaaa cggaagagga aatcgaggcg agcattcagg atggcgctta caaatcgcga
     5941 caggcgagtt tgtttcagca acagctcgat ttggccgtgg aactcgggtt caacgtcgta
     6001 attcaccagc gcgatgcttg ggcggacacg ctcgcgatta tgcagccgta caccgacaag
     6061 ttgcgcggcg tgtttcactg ttttggcggc acgctcgaac aggcgaacga agtactcgcc
     6121 ctcgaccatc tggtttcgtt cactggcatc gtgaccttca agaatggtgc ggccgtgcgc
     6181 gaggtcgcgg cacgcgttcc gctgtggaaa ttcatggtgg aaactgattg cccttatctc
     6241 gcaccggtgc cgtttcgcgg aaagcgctgc gaaccggcgc acacgcggct ggtcgcggaa
     6301 gcgattgctg cggcgcgcgg cgtttccttg caagaagtcg ccgaggccac gaccgagacg
     6361 gcagaaaaat tcttccgttt caatcgcgca tgaagatcga cattcaat
//
LOCUS       DASTDG010000093         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_640085, whole genome shotgun sequence.
ACCESSION   DASTDG010000093 DASTDG010000000
VERSION     DASTDG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640085"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFO30_02960"
     CDS             <1..213
                     /locus_tag="VFO30_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418275.1"
                     /translation="EKTKKEMQVIRQQFIRTGETRGDFVTVTNGLKAGQVVVSTGVFK
                     LRNGMPVVINNELAPKPQLAPSPAQT"
     gene            293..>2899
                     /locus_tag="VFO30_02965"
     CDS             293..>2899
                     /locus_tag="VFO30_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418276.1"
                     /translation="MKSFTDIFVRRPVLAMVVSFVIVIAGLQAIKTLNVRQYPRSENA
                     SITVRTVYVGASADLVRGFITSPLERAIAAADGIDYLQSTSQQGLSTITARLRLNYDA
                     NKALPQISAQVESVRNFLPVEAEVPTIKIEQADSQYASAYLSFTSDILEPNQVTDYLV
                     RIVQPRLSAIPGVQAADILGGRTFAMRIWLKPDRMAALNISPAQVRQALSANNYLAAV
                     GQTKGSLVKVNLTANTDLHTAEDFKRLVIRQENGRLIRLSDIADVVLGAEDYDTEVRY
                     SQQKAVFMGIRVLPNANSVDVIKRARTELEQIKRELPTGMEASVAYDATAYINDAMRE
                     VFKTLIETLGIVMLVIFLFLGSWRTVLAPIVAIPVSLIGAVFLMQVFGFTLNLLTLLA
                     IVLSVGLVVDDAIVVVENVERHLREGMRGVEAALRGARELVGPIIAMTLTLAAVYTPI
                     GLQGGLTGSLFREFALTLAGAVLISGIVALTLSPMICSKLLHRAGCDKGFSGVVNRSF
                     DRFRNAYGRMLDRTLQMRPAVYVVWIGLALLTYPMYKMSTHELAPTEDQGVIFGFIDS
                     PANATLDQLQPFADAAGKVFHSLPETNFSFQITSPDESFGGMVLKPWGVRKKPTKDFV
                     GPVMMGLMQIPGIEMFPLMPPALPGGGFMPVEFYICSTAEPERILELAEELKMRAIKA
                     GIFRFPPPIDTKIDQPESQIIIDHDKVASLGLDMKQIGADLSAMVGGNFVNWFNISGR
                     SYKVIPQVQRVDRLNADQLANMYVTGPSNQLISLSTIATIENRTVPRSLNRMQQLNAV
                     RLMGVPTRGLDQALRFLEDEASKMLPKGYHIDYTGESRQLRTEGNKFLPAFTLAIVMI
                     FLVLAAQFNSFRD"
BASE COUNT          596 a          848 c          832 g          623 t
ORIGIN      
        1 gagaagacga agaaggagat gcaagtgatt cgtcagcagt tcattcgcac cggtgaaacg
       61 cgcggcgatt tcgtgaccgt gaccaatggg ctcaaagccg gtcaagtcgt ggtgagcaca
      121 ggtgtcttta aacttcgcaa cggaatgccc gtagtaatca ataacgagct cgcgcctaag
      181 ccgcagcttg ctccgagccc ggcccaaacc tgatgcaaga tcgacaatcc acaatgtggc
      241 atgcgcttcc tgcgcatgac ttcatcggca ggatgccgat gccacgacgg agatgaaatc
      301 gttcaccgat atttttgtgc ggcggccggt gctggcgatg gtggtcagct ttgtcatcgt
      361 cattgctgga ctccaggcga tcaaaacgct gaacgtgcgc caatatccgc gcagcgaaaa
      421 cgcctcgatc acggtccgga ctgtttacgt cggcgccagt gcggatttgg tgcgcggctt
      481 catcacgtcc ccgctcgaac gcgccatcgc cgccgccgac gggatcgatt acttgcaatc
      541 gacaagccag caagggcttt ccacgatcac ggcgcgcttg cggttgaatt acgatgcgaa
      601 caaagccctg ccgcaaatca gcgcacaggt cgaatcagtc cgtaattttc tccctgtcga
      661 ggcggaagtt cccacgatca aaattgaaca ggctgattca cagtacgcct cggcctacct
      721 cagtttcacc tccgatattt tggagcccaa tcaggtcacc gactatctcg ttcggatcgt
      781 gcagccgcgt ctttccgcga tcccgggggt gcaggcggcg gacattcttg gcggccgcac
      841 ctttgccatg cgcatctggc tcaagccgga ccgcatggcg gcattgaaca tcagccccgc
      901 gcaggtccgg caggcgttat cggcgaacaa ttatctcgcg gccgttggcc aaaccaaagg
      961 ctcgctcgtg aaggtcaacc tgaccgccaa cactgatctg cacacggcgg aggatttcaa
     1021 acggcttgtc atccggcagg aaaacggaag gctcatccgg cttagcgaca tcgccgatgt
     1081 ggtgctcggc gcggaggatt acgataccga agtgcggtac tcgcagcaaa aagcggtgtt
     1141 catgggcatc cgcgtgctgc cgaacgcgaa ctccgttgat gtgatcaagc gtgctcgcac
     1201 cgagctggag cagattaaac gcgaattgcc cactggaatg gaggccagcg tcgcctacga
     1261 cgcgaccgct tacatcaacg acgccatgcg cgaagtgttc aagacattga tcgagacact
     1321 cggcatcgtg atgctggtca tctttttgtt cctcgggtcg tggcgcacgg tgctcgcgcc
     1381 gattgtcgcg attccggttt cactgattgg cgctgtcttt ctcatgcagg tgttcggatt
     1441 cacactcaac ctgctcacgt tgctcgccat cgtgctctcg gtcgggttgg tcgttgatga
     1501 cgcgattgtg gtcgtggaaa acgtcgagcg gcacctgcgc gaaggcatgc gcggagtgga
     1561 ggcggcgttg cgcggggcac gcgagctggt tgggccgatc atcgccatga ctctcaccct
     1621 cgccgccgtt tacaccccga tcggtttgca aggtggacta actggatcgc tattccgcga
     1681 attcgctctc acgctcgcgg gcgcggttct catctccggc atcgtggcgc tgactttgtc
     1741 gccaatgatc tgctcgaagc tgctccaccg cgccggctgc gacaaaggat ttagcggagt
     1801 ggtcaacagg agctttgatc ggtttcgcaa tgcctatggc aggatgctcg atcgcacctt
     1861 gcagatgcgt ccggctgttt acgtcgtctg gatcggtctc gcgttgctca cctatccaat
     1921 gtacaaaatg tccacgcacg agttggcgcc gacagaagat cagggcgtga tctttggttt
     1981 catcgattca ccggccaatg cgacgctcga tcagctgcaa cccttcgccg acgcggcggg
     2041 caaggttttc cacagtcttc cggagaccaa cttctcgttc cagatcacgt cgccggacga
     2101 gagcttcggc ggaatggtcc tgaaaccatg gggcgttcga aagaagccga ccaaagactt
     2161 tgtcgggccg gtgatgatgg ggctgatgca gatcccgggc attgaaatgt ttccacttat
     2221 gccgccggca ctgccgggtg ggggcttcat gccggtggag ttttacatct gttccaccgc
     2281 cgaacctgag cggattctcg aactggcgga ggaattgaaa atgcgcgcga taaaggccgg
     2341 cattttccgg tttccaccgc caatcgacac caagatcgac cagcccgaat cgcagatcat
     2401 tatcgatcac gacaaggtgg cttcgctcgg tctcgatatg aagcagatcg gggccgatct
     2461 ctccgccatg gtcggcggca attttgtcaa ctggttcaat atctccggtc gcagttacaa
     2521 ggtgattccg caggtgcagc gtgtcgatcg gttgaacgcc gaccagcttg ctaatatgta
     2581 cgtgactggc ccaagcaatc agctcatctc gctgagcacg atcgcgacga ttgaaaaccg
     2641 cacggtgcca cgctcgttaa accgcatgca gcagctcaat gccgtccgcc tgatgggcgt
     2701 gccgacgcgg ggtctcgatc aagcgctccg gtttctcgag gacgaagcca gcaaaatgct
     2761 gccaaaaggt taccacatcg attacaccgg cgaatcgcgc cagctccgga ccgaaggcaa
     2821 caaatttctg ccggccttta ctctcgccat cgtgatgatc tttttggtgc tcgccgcgca
     2881 gttcaacagt ttccgcgat
//
LOCUS       DASTDG010000094         9001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1442483, whole genome shotgun sequence.
ACCESSION   DASTDG010000094 DASTDG010000000
VERSION     DASTDG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442483"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /gene="rpsU"
                     /locus_tag="VFO30_02970"
     CDS             <1..247
                     /gene="rpsU"
                     /locus_tag="VFO30_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9418277.1"
                     /translation="PARLGSRVAGDEELIYPMPEVIVRKGEPVDRALKRLKSKLEAEG
                     ILEEVRRLRAFETPSQKSRRKAKANAKRARTRFRFNP"
     gene            complement(327..1931)
                     /locus_tag="VFO30_02975"
     CDS             complement(327..1931)
                     /locus_tag="VFO30_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9418278.1"
                     /translation="MPKCDISFPIPLPDDPHKWEGWSNYRSANFYERLCLDPGSNPSN
                     ELIEERCRELLRWWQRKLPLKNQPSNPIAQLLRAGLDESSRYLTEARVELLDPERRRK
                     LDEEIAEQEREEALLEFEKYVAFTVADGTLTADEEKNLIRFGMEHGLSQDVIMAEIDA
                     KLLETGAQRVQAPPPVAPSGALQAQSPGSAAPQEEFLRMLRLSGLDSFSLSDDQRDTF
                     IDIAENLGLDPGEAEDLVDLYIEEADAKSQPLPKPPPIRIQPKTPEPKEATTTRAPAK
                     PAIEINPAVERKRYINFKNSLGVEMLLVPSGEFVMGSDAFDASPHERPLTRVTVSRFY
                     MSRYLVTNAIYEQFDPSHARKRMRGAGDRYPVVYVSSLDAIKFCQWLSTRERKRYRLP
                     TEAEWEYAARGTDGRRYPWGNYEGRGDLANFADRNTVFAWSDRDIDDGYAETSPVGAY
                     PLGASAFGMEDMAGNVWEWCSDYFASYTTASKVNPTGPAAGTRRVYRGGSWKSRFNSL
                     RATVRNSNVPSFSCNDLGFRVVCECD"
     gene            complement(1932..3494)
                     /locus_tag="VFO30_02980"
     CDS             complement(1932..3494)
                     /locus_tag="VFO30_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9418279.1"
                     /translation="MTHRKAVGIDLGTTFSAVAHIDAYGKPQIIPNAESERITPSVIL
                     FDGSNVVVGTSAKNSAVAEPEKIVDFIKREMGKPKEQFHREFNGVIYSAEELSALIVK
                     KLIVDSERYLKEPVTDAVITVPAYFNDAERTATITAGQLAGLNVLQIINEPTAAALAY
                     GLDKLEEDQTVFVFDLGGGTFDVTIMRIEKQQLHIVATNGDHRLGGKDWDDVIVNYVA
                     EEFDRGHGENPLLDLQSYQDLQTRAVAAKLQLSSRKRTTIVHNHHGKSVKVELTRQDF
                     EKRTRHLIEKCKAICEIVMQEAKMTWDKIDKTLLVGGMTRMPMVREMIAQLSTAPLIS
                     DVNPDEAVAIGAAIQATLSMLHEEDVTGERVLGEGTRQQFSCRDGRLIQVNDITSHTL
                     GVVLWDEANLEEYVFPMIRKRTAIPATAKNSFGTASANMQHALVKVVEGESTMPADCT
                     PLGLCDVELPPFLPKGSPVELTYRYNANQVLEVSVEACGNQSKVKIERNTGLAPGEMD
                     KAIAGLGKLNVE"
     gene            complement(3643..4380)
                     /gene="grpE"
                     /locus_tag="VFO30_02985"
     CDS             complement(3643..4380)
                     /gene="grpE"
                     /locus_tag="VFO30_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9418280.1"
                     /translation="MSNVVEATDEFATAMQQLSAEAEKSLPLGQPEAAAQLPSASDQN
                     LLRVLQELSALSGRMQNTESALAERIEQLAKTLSPAEQVNIMVSRFEKIDEQLASIRS
                     TESVNQKLFDSLHAELRAYRDNFLHDSLQKPFIHDLVLLFDHLTSLHEQLRTAAQEKG
                     KRSPVCQWRDHLENAIASLVEVLHRLEVREIEPKETVDRALHRVVSFEPADYPEEDGR
                     IVMRVRRGFLWRGKLIRPEEVIAKRFN"
     gene            complement(4377..5924)
                     /gene="cobA"
                     /locus_tag="VFO30_02990"
     CDS             complement(4377..5924)
                     /gene="cobA"
                     /locus_tag="VFO30_02990"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET9418281.1"
                     /translation="MSRSTSKSTTKAGKVYLVGAGPGDLGLVTLRAKHCIELADVIIY
                     DHLANPEMLGWAREDAEIVYAGKRAGEHALTQQQINALLVENARAGKQVARLKGGDPF
                     VFGRGAEEAQAVVEAGFEFEIVPGITSAIAGPAYAGIPVTHRAQNSHVTFFTGHEDPA
                     KTTSAIDYGALARLGGTQVMLMGVERIESIAREMLKQGVREDLPVALVRWATTGRQET
                     LTGTLSNIAQRVLECGFEAPAVAIFGDVVALRQNLKWYENRPLSGKRIVVTRTRKQAS
                     ELSERLRALGADVIELPTIRIEPPSDLREFAELVQDAHAYDWIVFTSPNGVEAFFNLF
                     FKIYDDAREIGGAKIAAIGPATAQRVKDFHLHVDLQPEEFVAEAVVREFQKQGDIENL
                     RILVARAEKARDVLPKQLANLGAIVDEAFAYRTVAETRDSTGARRRLLEEGADLITFT
                     SSSTVENFLALGLPWPKRMQVATIGPITSKTARERGLTIAVEARRHDVPGLVEAIQKF
                     YGTGGGE"
     gene            complement(6036..6956)
                     /gene="hemC"
                     /locus_tag="VFO30_02995"
     CDS             complement(6036..6956)
                     /gene="hemC"
                     /locus_tag="VFO30_02995"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9418282.1"
                     /translation="MKIVIGTRGSELARTQAQMVSDVLRGRWSQLTIETKVIRTSGDE
                     RKIDMETSRAGRKGLFTAEIERALLAEDVDLAVHSAKDLPSELSAGTDIAAVLPRAPT
                     DDVLAAIEPWNMESLPGSAIVATGSVRRKHQLRWKRPDLDIVDLRGNVPTRLRKLATN
                     QWHAIVLARAGLDRLGHNIASGHIYFEGSEFFTEVLPEEIFLPAGGQGVIGIQIRSKD
                     ERAQLLLEPLNDFDTRLCLRAEREFLRLLHGDCNQPVGVLATINATVMKMRAQVFDLE
                     ATTPREAVVEGPSKDAERLAAQLLREINVQ"
     gene            complement(7034..8098)
                     /gene="hemA"
                     /locus_tag="VFO30_03000"
     CDS             complement(7034..8098)
                     /gene="hemA"
                     /locus_tag="VFO30_03000"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9418283.1"
                     /translation="MKLICIGLSHHTADVQTREQFVGSSVAESALRACGCAEALLLTT
                     CNRVEVYGSAGRPVKTAEVVRCLTRKIDIKCDASRFYRYEDADCAQHLFRVASGLDSM
                     VVGETEILGQAKNAYESARASGSAGPYLHRLFQRAFRVAKQVRTHTEITRGAVSVASV
                     AVDLAGKIFGDLRSCQVLVLGAGETSERTARALTSRGSTDVRVSNRSLDRAEELAHAV
                     GGRAVPFEEWPEQCREVDILMTSTSSEGPLVTREVLAPIVRERPDRPLFIIDIAVPRN
                     VDPAVNELGGVYLYDIDSLRSVAEQSLARRRQQIAAAEEIIAEHVAEFGAQIARGLNW
                     QLRTADHPSVRDGSLRTSEL"
     gene            complement(8095..8886)
                     /gene="ccsA"
                     /locus_tag="VFO30_03005"
     CDS             complement(8095..8886)
                     /gene="ccsA"
                     /locus_tag="VFO30_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9418284.1"
                     /translation="MDRNLLIVATLCFLAAVVRTGLELRSGVFRPMRFNFFAIGLGFI
                     FQTAFLSVRGHGLGRCPLTNLFEVLIFLCWSIALIYLLIGPAYRLSLMGAFTAPLIFT
                     IQTFALVAKIDNPHVARVPANAWLEFHASMSLVAYGAFALACIAGVMYLVQERQLKTH
                     HLHSIFYHLPPLTNLFGAMVRLLWVGFVLYTIGLASGFFVGQPLPWGQIVCAIGVWVL
                     YAAILHGRHLRRLAPKRVAALCIIGFSAAITLLWGISFSAEVHPP"
BASE COUNT         1780 a         2773 c         2567 g         1881 t
ORIGIN      
        1 cccggctaga ctcggctctc gggtcgcagg agatgaggag ctgatttatc ccatgccaga
       61 agtgatcgtt cgcaaaggtg aaccggtgga tcgcgctttg aaacggctca agagcaagct
      121 cgaagcagaa gggattctcg aagaggtgcg gcgcttgcgc gcgttcgaga ccccgtcgca
      181 aaagagccgc cgcaaagcca aggcgaacgc caaacgcgcg cggacgcgct ttcggtttaa
      241 tccgtaaaat cgatacccgt tgaagcgatg aagcgttgaa gtggatcgat gctttaaggt
      301 tcaagcttca acgcattcac gtcgcatcaa tcgcactcgc agacgacgcg gaagccgagg
      361 tcgttgcagg agaagctagg cacatttgag ttgcgcacgg tcgcgcgcag gctgttgaag
      421 cgcgatttcc agctgccacc gcggtagacg cggcgcgttc cggcggcggg gccggtggga
      481 ttcactttgg acgcggtggt gtagctggca aagtaatccg agcaccattc ccacacgttg
      541 ccggccatat cttccatgcc aaatgcgctc gcgccgagcg gatacgcacc gacaggcgat
      601 gtttctgcat aaccgtcgtc gatgtcgcga tcgctccagg caaacacggt gttacggtcg
      661 gcgaaattgg cgaggtcgcc gcggccttca taattgcccc agggataccg gcgcccgtcg
      721 gtgccgcgcg cggcgtattc ccattccgct tccgtcggca gccgatagcg cttgcgctcg
      781 cgcgtgctca gccattggca gaatttgatt gcgtcgaggc tgctgacgta gacgacagga
      841 tagcgatcac ccgctccgcg catgcgtttg cgcgcgtgtg agggatcgaa ctgctcgtaa
      901 atggcgttcg tcacaagata ccgcgacatg taaaagcgac tgacagttac gcgcgtgagt
      961 ggccgctcat gtggtgaggc atcgaacgca tcgctgccca taacaaactc tccggagggg
     1021 acgaggagca tttccacccc gagtgagttt ttgaaattga tgtagcgctt gcgttcaacc
     1081 gctggattga tttcgattgc cggctttgcc ggagctcgcg tcgttgtggc ctcttttggc
     1141 tccggtgtct tcggttgaat ccgaatcggt ggcggcttcg gcagaggctg ggacttggca
     1201 tccgcctctt caatgtacag atcgacaagg tcttccgctt caccggggtc gaggccgagg
     1261 ttttcggcga tgtcgatgaa cgtgtcgcgt tgatcgtcac tcaggctaaa gctgtcgagg
     1321 ccgctgagac gcagcatgcg cagaaattct tcctgtggag cggccgatcc gggcgattgc
     1381 gcctggagcg cgccggacgg cgcgaccggg ggcggcgcct ggacgcgctg cgcgccggtc
     1441 tcgagcaact tcgcgtcgat ttccgccatg atcacgtctt ggctaaggcc gtgttccatg
     1501 ccaaaacgga tcaggttttt ttcttcgtcc gcagtcaatg ttccgtcggc tacagtaaag
     1561 gcgacatact tttcgaactc gagcagcgct tcttcgcgct cctgttccgc gatctcctcg
     1621 tcgagtttac ggcgccgctc cgggtcaagc agctcgacgc gcgcttcggt caggtagcgc
     1681 gacgactcgt cgagcccagc gcggagcaat tgtgcgatcg gattgctcgg ttggttctta
     1741 agcgggagtt tccgttgcca ccagcgcaac agctcgcggc agcgctcctc gatcagttca
     1801 ttgctcggat tgctccccgg atcaagacag agccgctcgt aaaagttagc cgagcggtag
     1861 ttgctccaac cttcccattt gtggggatcg tcggggagcg gaatgggaaa gctgatgtcg
     1921 catttaggca tttactcgac gttcaactta ccgagaccag cgatggcttt gtccatttcg
     1981 ccgggcgcga gcccagtgtt ccgttcgatc ttcaccttcg actgattgcc gcaagcctcc
     2041 actgagacct cgagcacctg attggcattg tatcgatatg tcagctcaac cggcgaacct
     2101 ttgggcaaaa acggtggcaa ctcgacatcg caaaggccga gcggagtgca atcggccggc
     2161 attgtacttt cgccctcgac gactttgacc agcgcgtgtt gcatgttcgc gctcgctgtg
     2221 ccgaatgaat ttttcgccgt cgccggaatg gccgtgcgct tcctgatcat cgggaaaacg
     2281 tattcctcga ggttcgcttc atcccacaag accacgccga gcgtgtgcga ggtgatatcg
     2341 ttgacctgaa tcagccgtcc gtcgcgacag gagaattgct ggcgcgtgcc ttcgccgagc
     2401 acgcgctcgc cggtgacatc ttcttcgtgc aacatcgaca atgtcgcttg aatggccgca
     2461 ccgattgcga ccgcctcgtc cgggttcacg tcgctgatca gcggcgcggt cgacagctgc
     2521 gcgatcattt cacgcaccat cggcatgcgc gtcatgccgc cgacgagcaa tgtcttgtcg
     2581 atcttgtccc acgtcatctt cgcctcttgc atgacaattt cgcagatcgc cttgcatttc
     2641 tcgatcaggt ggcgcgtccg cttctcgaaa tcctggcgcg tcaactcgac cttaacgctt
     2701 ttgccatggt gattgtggac gatggttgtg cgtttgcggc tggagagctg aagtttcgcc
     2761 gccaccgcgc gcgtctgcag gtcctgataa ctttgcaggt cgagcagcgg gttttcgcca
     2821 tgcccgcggt cgaactcctc agccacgtaa ttgacgatga cgtcgtccca gtccttgccg
     2881 ccgaggcggt ggtcgccgtt tgtcgcgacg atgtgcagct gttgcttctc gatccgcatg
     2941 atcgtgacgt cgaacgtgcc gccgccgaga tcgaacacga aaactgtctg gtcctcctcc
     3001 aatttgtcca gaccgtaggc cagagccgct gccgtcggtt cgttgatgat ttgtaaaaca
     3061 ttcaagcctg cgagctggcc ggcagtaatc gtggcagtac gctccgcgtc gttaaagtac
     3121 gccgggacgg tgatgaccgc gtcggtcacg ggctccttca gatagcgctc cgaatcgaca
     3181 atgagttttt tcacgatcag cgcggagagt tcttcggccg agtagatgac gccattgaat
     3241 tcgcgatgga attgctcttt tggcttgccc atctcgcgct tgataaaatc gacaatcttc
     3301 tccggctcag cgaccgcgct gtttttggcg ctcgtgccga cgaccacgtt cgaaccgtcg
     3361 aagagaatga ccgaaggcgt gattcgttcg ctctcggcgt tcgggatgat ttgaggttta
     3421 ccgtatgcgt cgatgtgcgc gaccgctgag aaggtcgttc ccaagtcaat gccaactgct
     3481 ttgcgatgag tcatatctcg aatttagaaa tccggaactg attgaggacg ttccgcgggc
     3541 aggactattg cagttttcat gcccggcacg gattagaaat gcagggtcaa gagttgcgcg
     3601 actgtccatc cgcacaagat cgacattggc cgccgcgcga cctcagttga accgcttcgc
     3661 gatgacttct tcgggccgaa tgagtttccc gcgccagagg aagccgcgcc gcacgcgcat
     3721 gacgatgcgt ccatcttcct ccggataatc ggcgggctcg aagctgacga cgcgatgcag
     3781 cgcgcgatcg acggtttcct tcggctcgat ttccctgacc tcgagccgat gaagaacctc
     3841 aaccagcgag gcgatcgcgt tctcgaggtg atctcgccat tggcagaccg ggctgcgctt
     3901 gcctttctcc tgtgcggccg tgcgcaactg ctcgtgcaag ctggtgaggt gatcgaacag
     3961 taacaccagg tcgtggatga acggtttttg gagcgaatcg tgcaggaaat tgtcgcgata
     4021 cgctcgcagc tcagcatgca gcgagtcgaa tagcttctgg ttcacgcttt cggtgctgcg
     4081 gatcgacgca agttgctcgt cgattttctc gaagcgcgaa accatgatgt tgacctgttc
     4141 ggccggcgaa agggtcttcg cgagctgctc gatacgctcg gccaacgcgc tttccgtgtt
     4201 ctgcatccgc ccgctcagcg cagaaagctc ctgcaatacg cgcaataagt tttgatcgct
     4261 cgccgatggc aattgcgcgg ccgcttcagg ttgtccaagc ggcagcgatt tttccgcttc
     4321 tgcgctcaac tgctgcatcg cggtggcgaa ttcgtcggtc gcttccacaa cgttgctcat
     4381 tcgccgcctc ctgttccata aaatttctgg atcgcctcca cgagacccgg cacgtcatga
     4441 cggcgcgctt cgacagcgat ggtcaatccc cgttcgcgcg ctgttttcga tgtaatcggt
     4501 ccgatggttg cgacctgcat ccgcttcggc cacggcaaac caagcgcgag aaaattttcc
     4561 acggttgagg agctggtgaa cgtgatcagg tcggcgccct cctccaaaag acgtcggcgc
     4621 gcaccggtgc tgtcgcgcgt ttcggccacc gtccgatatg cgaaggcttc gtccacaatt
     4681 gctccgagat tggcaagctg cttcggaagc acatcccgcg ccttctccgc ccgcgcgacc
     4741 aggattcgca ggttctcgat atcgccttgc ttctggaatt cgcgcaccac cgcctcggcc
     4801 acgaactcct cgggctgaag atcgacatgc aggtgaaaat ctttcacccg ctgcgcggtg
     4861 gctggcccga tcgccgcgat tttcgcgccc ccgatctcgc gcgcgtcgtc atagattttg
     4921 aaaaataaat tgaagaacgc ctcgacccca ttcgggctgg taaaaacgat ccagtcgtac
     4981 gcgtgcgcgt cctgcacgag ctcagcaaac tcgcgcaagt cgctcggcgg ctcgattcgg
     5041 atagtcggca actcgatcac atccgcgccg agcgcgcgca gccgctcgct caactcgctt
     5101 gcctgcttgc gtgtgcgcgt gactacgatc cgtttacctg acaatggccg attttcgtac
     5161 cacttgagat tttgccgcag cgcgacgacg tcgccgaaga tcgcaaccgc cggcgcttcg
     5221 aagccgcact cgagcactcg ttgcgcgata ttcgagagcg tgccggtaag ggtctcctgg
     5281 cgtccggttg tcgcccaacg caccagcgcc acgggcaaat cttcccgtac gccctgcttc
     5341 agcatctccc gcgcgatcga ttcgatccgc tcgacaccca tcaacatgac ttgcgtgccg
     5401 ccgagcctag ccagcgcgcc ataatcgatc gcgcttgttg ttttcgccgg atcttcgtgt
     5461 ccggtgaaaa acgtcacgtg cgagttttgc gcgcgatgcg tcactggaat gcctgcgtag
     5521 gctgggccgg caatggccga agtgatcccc ggcacgatct caaactcgaa gcctgcttcg
     5581 acaaccgcct gcgcttcttc cgcgccccgg ccgaagacaa atggatcgcc gcctttcaag
     5641 cgggcgacct gcttgcctgc acgggcgttt tcgacgagca acgcattgat ttgttgctga
     5701 gtgagtgcgt gctcaccggc gcgtttgccg gcgtaaacga tctcagcatc ttcccgcgcc
     5761 cagccgagca tttccgggtt ggccaaatgg tcgtaaataa tgacgtcagc cagctcaatg
     5821 cagtgcttcg cgcgcaaggt caccaggcca agatcccctg gccccgcacc gacgagataa
     5881 accttgcccg ctttagtcgt acttttgctc gtgctcctgc tcatgccccg agtaacgacg
     5941 tgccctcacc tcaatcctct ccctcaggga gaggcggaac agcctccgca gagctacggc
     6001 tcggcgagcg cgaagcgcca ggtgaggctt gcttttcatt gaacatttat ctcccgtagt
     6061 aattgcgccg ccagccgctc agcgtctttg cttggccctt cgacgaccgc ttcgcgcggc
     6121 gtcgttgctt ccagatcaaa aacctgcgcg cgcatcttca taactgtcgc attgattgtc
     6181 gccaagacgc caactggctg attgcagtca ccatgcagta agcgcagaaa ttcgcgctcc
     6241 gcccgcaaac aaagccgcgt gtcgaaatcg ttgagcggct cgaggagcag ctgcgcccgc
     6301 tcgtctttgc tgcgaatctg gatcccaatc accccctgcc ctcccgccgg taagaaaatc
     6361 tcctctggaa gaacctccgt gaaaaactca cttccctcaa aataaatgtg tcccgaagcg
     6421 atattgtgac cgaggcggtc cagcccggcg cgcgcgagaa cgatggcatg ccactggttg
     6481 gtcgcgagct tgcgcagccg cgtcggcacg ttgccgcgaa gatcgacgat atccaaatcc
     6541 ggccgcttcc aacgcagctg gtgtttgcgc ctcacgctgc cagtcgcgac gatcgcactg
     6601 ccgggtagcg actccatatt ccacggttca atcgccgcga gaacatcatc cgtcggcgcc
     6661 cgcggcagaa cagcggcgat atccgtgccg gcgcttagct cgctcggcaa atccttggca
     6721 ctgtgcaccg caagatcgac atcttcggcc aatagcgccc gttcaatctc ggctgtgaac
     6781 aatcctttgc gtccggcgcg gcttgtttcc atgtcgatct tgcgttcgtc gccagaagtc
     6841 cgaatcactt tcgtttcaat cgtcaattgc gaccagcgcc cacgcagaac gtcgctcacc
     6901 atctgcgctt gggttcgcgc caattcactc ccgcgcgtcc cgatcacaat cttcatccga
     6961 gacggagcat ccatcgttgt ttctgtagcg gcggtctctg accgtcgcaa atagtgacgc
     7021 gcagggagcg ccgctacagc tcggaagtgc gcaacgagcc gtccctaact gatggatgat
     7081 cagctgtccg caactgccag tttaacccgc gcgcgatttg cgcaccaaat tcggcgacgt
     7141 gctcagcaat gatctcttct gcggcggcaa tttgctgtcg ccgccgcgcg agcgactgtt
     7201 ctgcaaccga ccgcagcgag tcgatgtcgt agagatacac tcccccgagc tcgttcacgg
     7261 ctggatcgac attacgcggc acggcgatgt cgatgatgaa aagcggtcgg tccggtcgtt
     7321 cgcgcacaat cggcgcaagc acttcgcgcg tcaccaacgg cccctcagat gaagttgaag
     7381 tcatcaggat gtcgacctcg cggcattgct ccggccattc ctcgaaagga acggcgcgac
     7441 caccaacggc gtgggcgagt tcctccgcgc ggtcgagcga gcggttgctt acgcgcacgt
     7501 ccgtcgaacc gcgcgaagtc agagcgcgtg ctgttcgttc gctcgtttct ccggcaccga
     7561 gcactagaac ttggcagctt cttaggtcgc cgaaaatttt tcccgcgaga tcgaccgcca
     7621 cggatgcgac cgagaccgcg ccgcgcgtaa tctcagtgtg ggtgcgcacc tgtttggcca
     7681 cgcgaaacgc gcgctgaaac agacggtgca gatacggtcc tgcggaaccg gacgcgcgcg
     7741 ccgactcgta cgcattcttc gcctggccca gaatctctgt ctcaccgaca accattgaat
     7801 caagacccga cgcgacacga aatagatgct gcgcgcagtc ggcgtcttca taacgataaa
     7861 atctcgaggc gtcgcacttg atatcgatct tgcgcgtgag gcaccggacg acctctgcgg
     7921 ttttgaccgg tcgcccggcc gagccgtaga cttcgacacg attacaggtt gtcagcagca
     7981 atgcttcggc gcagccacac gcgcgcagcg cgctttcggc cacactactc ccgacgaact
     8041 gctcgcgggt ttgcacatcg gccgtgtgat gactcagccc aatgcaaatc agtttcatgg
     8101 cggatgcacc tccgcgctga aactgattcc ccatagtagc gtaatcgccg cgctgaagcc
     8161 gataatacaa agcgcggcca cgcgcttcgg cgccagccgc cgcaaatgtc ggccgtgcaa
     8221 gatcgcagcg taaagaaccc agacgccgat cgcgcaaaca atttgccccc acggcaacgg
     8281 ttgaccgacg aagaatccgc tcgccaggcc aatcgtgtac aaaacgaagc cgacccaaag
     8341 caatcgaacc atcgcgccaa agagattcgt cagcggtggc aagtgataga agatcgagtg
     8401 caagtggtgc gtcttcagct ggcgctcctg cacgaggtac atcacccccg cgatgcaagc
     8461 cagcgcgaac gcgccgtagg ccacgagcga cattgaagcg tgaaattcca gccacgcgtt
     8521 cgccggcacc cgggcgacgt gcggattgtc aatcttcgct actaacgcaa acgtctggat
     8581 tgtgaagata agcggggctg tgaacgcgcc cataagcgag agccggtagg cagggccgat
     8641 gagcaaatag atcagcgcga tcgaccagca gaggaagatc agcacctcga acagattcgt
     8701 cagtggacag cggccaaggc catgcccgcg aaccgataga aatgccgtct gaaaaataaa
     8761 ccccagtcca atcgcgaaga aattgaatcg catcgggcga aaaaccccgc tgcgcagctc
     8821 gaggccagtc cgcaccactg ccgctagaaa acacagcgtc gcaacaatca aaagatttcg
     8881 atccacggcg cgatgcagaa ggtaacccta ctcccgcagc ctatcgagca cgaatactcg
     8941 ggctaagtgg gactatccag ccgacgagca gccccacggc cagagctggg acgcaataaa
     9001 a
//
LOCUS       DASTDG010000095         7889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1449312, whole genome shotgun sequence.
ACCESSION   DASTDG010000095 DASTDG010000000
VERSION     DASTDG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449312"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..318
                     /gene="infA"
                     /locus_tag="VFO30_03010"
     CDS             100..318
                     /gene="infA"
                     /locus_tag="VFO30_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9418285.1"
                     /translation="MPKEEQIVTEGTVTEVLPGTMFRVDLGGQRNVLAHISGKMRKHF
                     IRIVPGDKVSVELSPYDLTKARIIHREQ"
     gene            525..833
                     /locus_tag="VFO30_03015"
     CDS             525..833
                     /locus_tag="VFO30_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418286.1"
                     /translation="MAEQNEQGTSDARSKLESSKTHARKAAEDLRAAAGSIAGEYRGK
                     AEHALDEARGRAEQAWGDARNRARTLQEDAEEFVRENPTKAVFTALGIGFILGLIFRR
                     "
     gene            847..1278
                     /locus_tag="VFO30_03020"
     CDS             847..1278
                     /locus_tag="VFO30_03020"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9418287.1"
                     /translation="MAEEAARSQNPAGRAGLLDTVLALASAVAGFFENRFALIARDSK
                     VALVQLLVLVAFFGAAVLLCAFGYVFLLLSAIFGVAHLAGVSWLWTALAAAILHFIIA
                     LVCVLLARERLRRPLFRATASELNKDREWLKNLGRTTRPNS"
     gene            1236..1634
                     /locus_tag="VFO30_03025"
     CDS             1236..1634
                     /locus_tag="VFO30_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418288.1"
                     /translation="MAQEPRQNNSPEQLSAEIARTREAVTRNLTGLRYELDFPRKIRR
                     SFRQETMLWIGAAVAVGILIAVVPARTKKVYVEPKIGKKSKHRLLEAGFVLGALKIAA
                     SLLRPVIVDFVRARLIHVPPPRPLPAAKIW"
     gene            1736..2788
                     /locus_tag="VFO30_03030"
     CDS             1736..2788
                     /locus_tag="VFO30_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9418289.1"
                     /translation="MDEYLEVGQKAGASDVHLTVNAPPLWRLHGKLQPMWPDAPRLTA
                     QDTAALVAGFIPETNRIELAERGDTDFAYANRAARYRTSVVRQRLGTDIVFRVINTHV
                     RTMDELGLPQHLKLLTRYQNGLILATGSVGTGKSTTLAAMVEQVNMERREHIITLEDP
                     IEYIIESKNCHVTQREVFTHTKSFAAALRAALREDPDVIMVGEMRDLETISLAITAAE
                     TGHLVMATLHTSNASRTLDRLLDVFPSDQQEQVRVMVSESLRGIMSHQLIPRKDGTGR
                     VLALEILTNTPAVANVIREAKTYMLPGIIQTGKKQGMQLMDDTLLDLYQRGLISAEEA
                     YARVDQKDSMRQHLKG"
     gene            2795..3952
                     /locus_tag="VFO30_03035"
     CDS             2795..3952
                     /locus_tag="VFO30_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9418290.1"
                     /translation="MPYLDQYLNIVVRQGASDLHIGEGQPPKIRKHGDIMPIREQPIT
                     REEAMRMLSEVTGPRNWEIFDQRGDLDFAYEMDAASRFRCNYLKQANGYGAVFRLIPT
                     KIASLEELGIPVTAKQFAELRGGLVLVTGPTGSGKTTTQAALLDYINQNFSKHIVTIE
                     EPIEFVHEHKRCIITQREVPTHSATFPDALKASLREDVDALLLGEMRDLETISLALTA
                     AETGLLVFGTLHTNNARKTVDRMVDVFPAERQAQARAMLANSLRGVLAQLLLKRCDRP
                     GRIAVNEILLANPAVAAIIREGATQKLQDVIVSGKAQGMQFMDDAIWSLLEQGIVSPH
                     EAFMKAIDKNRFKPFLPAGEEHLADAAGSVPDDERRSPGNFVKRISRGARR"
     gene            4139..4750
                     /locus_tag="VFO30_03040"
     CDS             4139..4750
                     /locus_tag="VFO30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9418291.1"
                     /translation="MHSLLNSQVLVLNRLWQAVNICTARRAFALVYAGHAHVVSSDEA
                     NNFLTHDFDSWRDVSAAAPDNELVTTISWKIRVPRVIVLLFFDRLPKKEVKFTRHNVF
                     ERDKNTCQYCGHLFDRNDLNLDHVVPRDRGGLTTWENVVCSCIPCNTRKGNRLPQEAH
                     MTLIRKPKRPKWRPFVHVTFAATQHESWRHFVDLAYWNVELSD"
     gene            complement(4759..6213)
                     /locus_tag="VFO30_03045"
     CDS             complement(4759..6213)
                     /locus_tag="VFO30_03045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9418292.1"
                     /translation="MIPAPALEIAVLLFGMAILMLEAFAARLEKRTLAYIAVGSLTAV
                     LIATWFVNPAPPPESSTGFWSFYTADALSIFFKRFALITSIVVLLMMLDYAPIVQDEA
                     SGRPVLAEFFALPLFTCAGLMYLVSAIDFVMIFVSLELVTISFYILVSFRRRHAATLE
                     AGAKYLVLSALSTAFLVYGVAWVFGATGETNLQRITAALATPGASQGAALFGAVLVLV
                     ALGFKIAAVPFQIWVPDVYQGAPTPVTAFLSVGSKGAGFVVLLRVLRPFLMLPHMQRL
                     LILLALLTMIYGNLAALPQNNFKRLLAYSSIAHAGYLLIGVACFDGNSVVFYLTAYLL
                     MTLLSFAVLIIVERQSGDAIADFDGLSRRSPFLAFALLLGMLALAGLPFTAGFFGKFF
                     IFYAAVEQHQTALVAVGIVTVACGFYYYLKVVRAMYWQAPATNSALPISALSRATMTA
                     LIAGTIFFGVYPQPILRAIKPESEAIAVRSTINR"
     gene            complement(6210..7688)
                     /locus_tag="VFO30_03050"
     CDS             complement(6210..7688)
                     /locus_tag="VFO30_03050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9418293.1"
                     /translation="MLLLVLFAPIVAATLIFAGAPARKTALSASAFSLVATLAVFATF
                     PSAPRGFQNVVSLPISAEWGLSFTIGIDGLSLVMILLASIVTVAAVWFAGQIDRYENV
                     FYGCLLLISAGAIGAFASLDLFFFYAFHELALIPTFLLIGIWGTGNRVAAAWKITIYL
                     AFGSFILLIGLIMLYQSVPIGSRSFDIRALQGAAAAGQISADAQRHIYPLLLVGFGIL
                     ISLFPFHTWAPEAYAAAPAPAAMLHAGVLKKFGLYGLLRLALPLLPEGARYWTNLLIV
                     LLLGNIVYIGLVTIAQKRLDWMLGYSSVMHMGYIFLGIASANVLGTTGAAILIFAHGL
                     AIALLFALAGELRQRTASLEFTDLGGLAKVMPFAGLAFGLAAFAAVGLPGFANFAAEV
                     MVFFGAFRTGWEVQRFHIFQIATVLALWGVVISTVYMLRAYRRIFMGTLPERWKEVVD
                     LHRSLRVPVALLVAALLWFGFFPQTFVRLLAPALQLYVSATR"
     gene            complement(7688..>7889)
                     /locus_tag="VFO30_03055"
     CDS             complement(7688..>7889)
                     /locus_tag="VFO30_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9418294.1"
                     /translation="STQELLARVSAFTDRWLIDAAAVRGASSGTWGLGTLLRLFQVGN
                     LQAYSFLFGLGIVALIYFAVFR"
BASE COUNT         1851 a         2233 c         2359 g         1446 t
ORIGIN      
        1 cgatcactgt agctacgtcg gtgtgcgcgg tcgacggccc acggagccgt ggctacagcg
       61 cggttgagac acatctccgc tgcggtatct tttcatgcga tgccgaaaga ggaacaaatt
      121 gtgaccgaag gcacggtgac agaagtgctt ccggggacga tgttccgcgt tgacctcggt
      181 gggcagcgca atgtcctcgc ccatatctcg ggcaagatgc gcaagcattt catccgcatc
      241 gttcccggcg acaaagtgtc ggtggaatta tcgccttacg atctgacgaa ggcgcgcatc
      301 atccatcgtg agcagtgatc accggctggg ctgtccagcc gggcaagtat tggcagtatc
      361 gcggggacat atgtgagcag caagtacgtc cgacacttcc cagcggctgg agtctggctg
      421 cgccctgaag ctggacgtat cgcttatcgg aaagattcga ctgcgcgttt gcccgaaagg
      481 caaaccgcgc gcaatgttaa cggaaattca actggagacg accaatggct gaacaaaacg
      541 aacaaggaac gagtgacgca cgtagcaagc tggagagtag caagacacac gcacgcaagg
      601 cggcggagga tttgcgggcc gcggcaggtt cgatcgcggg cgaatatcgc gggaaagctg
      661 agcacgcgct ggacgaagca cgtggccggg ccgagcaggc gtggggtgac gcgagaaatc
      721 gcgcgcgcac tttgcaggaa gacgcagagg aattcgtccg agaaaatccg actaaggccg
      781 tgttcacagc gctgggaatc ggatttattc tcgggctgat tttccgtcgc taatccggtg
      841 cgctgaatgg ctgaggaggc ggctcggtcg caaaacccgg ccggccgcgc cgggttgctt
      901 gacactgtgc ttgcgctggc gagcgcggtc gccggatttt tcgagaatcg gttcgcgctg
      961 atcgcgcgcg attcgaaagt agcgctggtc cagctgctcg tgctggtggc gttttttggg
     1021 gcagcggtgc tgttatgcgc gttcggctac gtctttttac tgcttagcgc gatattcggc
     1081 gtcgcccatt tggcaggggt ctcatggttg tggacggctt tggccgcggc gattttgcac
     1141 ttcatcattg cgctggtctg tgttttgctg gctagagagc gcctgcgccg gccgctcttt
     1201 cgcgcaacgg catctgaact aaacaaggac cgcgaatggc tcaagaacct cggcagaaca
     1261 actcgcccga acagttgagc gcggagatcg cgcgcacgcg ggaggcggtt acgcgcaatc
     1321 tgaccggctt gcgctatgaa ctagactttc cgcgcaagat tcggcgttcg tttcggcagg
     1381 aaacaatgct ttggatcggc gcggccgtag ccgtgggcat tctgatcgcc gttgtgccag
     1441 cgcggacgaa gaaagtttac gtggaaccga agattggaaa gaaatcaaag catcgtctgc
     1501 tagaggccgg cttcgttctg ggcgcgctga aaatcgccgc gagtttgctt cgtccggtca
     1561 ttgtcgattt tgtccgcgcc cggctaattc acgtgccgcc cccgcgcccg ctgccggctg
     1621 caaagatctg gtgaaccggt tttcacgcgt tcgcggcggc ggacaattat cccggtagcg
     1681 gatcatgctc gcaaaggtcc agacaatatc gactcggccg cttccgcttc ggcatgtgga
     1741 tgaatatctg gaggtcggcc aaaaagccgg tgcctcggat gtgcatctca ccgtcaacgc
     1801 gccgccgctc tggcgtctgc atggaaagtt acagccgatg tggccggacg caccgcgtct
     1861 gacggcgcag gacaccgctg ctttggtggc cggtttcatt ccggaaacaa atcgaatcga
     1921 gctggctgag cgtggcgaca ccgatttcgc atacgcgaat cgcgccgcgc gctatcgcac
     1981 tagtgtcgtg cggcaacggc tcggcaccga catcgtgttt cgcgtcatca atacgcacgt
     2041 gcggacgatg gacgagctcg ggttgccgca gcatctcaag ctactgacgc gctatcaaaa
     2101 tgggttaatt ctcgccaccg gttcggtcgg caccggcaaa tcgacgaccc tcgcggcgat
     2161 ggttgaacaa gtgaacatgg aacggcgcga acacatcatc acgctggagg acccgattga
     2221 gtacatcatt gagtctaaaa attgccacgt cacgcagcgt gaagtcttca cgcatacgaa
     2281 atcgttcgcc gcggcattgc gcgctgcgct gcgggaagac ccggacgtga tcatggttgg
     2341 cgagatgcgc gatttggaaa cgatttcgct tgccattacc gcagcagaga ccgggcactt
     2401 ggtgatggcg acgttgcata ccagtaatgc ctcacgcacg ctcgatcgct tgctcgacgt
     2461 ctttccatcc gaccaacagg agcaggtccg ggtgatggta agtgagtcgc tgcgcggaat
     2521 tatgagtcac cagctcattc cccgaaaaga cgggaccggc cgtgtgctgg cattggaaat
     2581 cctgacgaac acgccggccg tggcgaacgt gatccgcgaa gcaaagacct atatgctgcc
     2641 cggcatcatc cagactggca agaagcaagg catgcaactg atggacgata cacttctcga
     2701 tctttaccaa cgcggcctaa tcagcgccga ggaggcctac gcgcgtgtcg accaaaaaga
     2761 ctccatgcgc cagcatctca agggatgaac gtcaatgccg taccttgatc aatacctcaa
     2821 catcgtcgtc cgccaaggcg cgtccgatct gcacatcggc gaagggcagc cgcccaaaat
     2881 ccgcaagcac ggcgatatta tgccgattcg cgagcagccg atcacgcgag aggaagcaat
     2941 gaggatgttg agtgaagtca ctggcccgcg gaactgggaa attttcgacc aacgcggcga
     3001 tctcgacttc gcgtacgaaa tggatgcggc ttcgcgcttc cgctgcaatt acctgaagca
     3061 ggccaacggt tacggcgcgg tctttcgttt gatcccgacc aaaatcgcgt ctctcgagga
     3121 actgggcatt ccagtaactg cgaagcagtt cgcggagctg cgtggcggtc ttgtcttagt
     3181 caccggcccg actggttcgg gcaaaacgac aacgcaggca gcgctgctcg attacatcaa
     3241 ccaaaatttt tcgaaacaca tcgtcacgat cgaggagccg atcgagttcg tgcacgagca
     3301 taagcgctgc atcatcaccc aacgcgaggt gccgacccac tcggctacgt ttcccgacgc
     3361 gttgaaggcg tcgttgcgcg aagacgtgga cgcgctcctc ctcggggaaa tgcgcgacct
     3421 ggaaacgatt tcccttgcgc tgaccgccgc tgagaccggc ttacttgtat tcggcacgct
     3481 gcataccaac aacgcgcgta agacggtcga ccgaatggtc gacgtgtttc cggccgagcg
     3541 tcaggcgcag gcgcgagcga tgctggcgaa ctcgttgcgg ggagttctcg ctcaactact
     3601 gctgaagcgg tgcgaccgcc ccggtcggat cgcggttaat gaaatcctcc tcgcgaatcc
     3661 cgctgtggcc gcgatcattc gcgagggcgc gacccagaaa cttcaggacg tgattgtttc
     3721 cggcaaggcc cagggtatgc aattcatgga cgacgccatt tggtcgctgt tggaacaagg
     3781 catcgtctcg ccgcacgaag ctttcatgaa agcgatcgat aaaaaccggt tcaaaccatt
     3841 tctgccagcg ggcgaggaac atctggccga cgctgccggc tccgttccgg acgacgagcg
     3901 gcggtcgccc ggcaattttg tgaaacgcat ttcacgcggc gcacggcgct gaactatttc
     3961 ccgtcgaaaa tctcgccgtt tagctcgatc gattccacgc ctaaatttac ctctgtaacc
     4021 tcacagcgtc atgccaaagc cgccgtccgt tgacactgaa atagccgagt ggtaccgtcg
     4081 cggcactttg cggaattgac ttcatgagag ggcgttaaga tcgaaaatcg caagtgaaat
     4141 gcactcgttg ttgaacagtc aggttttggt gctcaaccgc ctctggcagg cggtcaacat
     4201 ctgcacggcg cgccgcgctt ttgccctggt ttacgctggc cacgcgcacg ttgtttcctc
     4261 cgacgaagcg aacaacttcc tgacacacga tttcgacagt tggcgcgacg tttcagccgc
     4321 cgcgccggac aacgaactgg tcacaaccat ttcgtggaag atccgagtgc cgcgggttat
     4381 cgtgctgctc ttttttgatc gcctgccgaa gaaggaggta aagtttacga ggcataacgt
     4441 gttcgagcgc gacaaaaaca cgtgccagta ctgcggtcac ctgtttgatc gcaatgacct
     4501 gaatctcgac catgtcgtgc cgcgagatcg cggtggccta acaacttggg aaaacgtcgt
     4561 ttgctcctgc attccatgta acacacgtaa aggcaaccgc ctcccgcagg aagcgcacat
     4621 gacgctcatt cgaaagccaa agaggccgaa atggcgcccg tttgtgcacg tcacgttcgc
     4681 ggcgacgcag cacgaaagct ggcgacactt tgtcgatctt gcctattgga atgtcgagct
     4741 gagcgactag gcaatcgctt accgattaat tgtcgatcgt acggcgatag cttccgattc
     4801 aggtttgatc gcgcgcagga tcggttgtgg ataaacgccg aagaaaatcg tgcctgcaat
     4861 cagcgcagtc atcgtggcac gcgagagcgc actaatcgga agagcggaat tggtcgcggg
     4921 tgcctgccag tacatcgccc gaaccacttt caaatagtaa taaaatccgc atgctacggt
     4981 gacaatcccc acggccacga gcgcggtttg atgttgttca acggcggcgt agaaaatgaa
     5041 aaacttgccg aagaagcccg ccgtaaatgg aagtcccgcg agcgcgagca tgccgagtag
     5101 aagcgcgaag gcaaggaagg gcgagcgcct cgacagaccg tcaaaatcgg cgatggcgtc
     5161 gccgctttgc cgctcgacga tgataagcac ggcaaaactg agcagcgtca tcaacagata
     5221 cgccgtcaaa taaaacacga ccgaattgcc gtcgaaacac gccacgccga tgagcagata
     5281 acccgcatgc gcgatgctgg agtaagcgag cagacgtttg aaattgtttt gtggcagcgc
     5341 ggcgaggttg ccataaatca tagtgagcag cgccaggaga ataaggagac gctgcatgtg
     5401 cggcagcatc aggaacggcc gaagcacgcg cagcaggacc acgaaaccgg caccctttga
     5461 tcccactgat aaaaatgctg tgaccggcgt cggtgcgcct tgatatacat caggtaccca
     5521 gatttgaaat ggcacggccg cgattttgaa cccgagcgcg accaggacga gcactgcgcc
     5581 gaagagtgcg gcgccttggc ttgcaccggg ggttgctagc gcggcggtaa ttcgctgcag
     5641 gttcgtttcg ccggtcgcgc cgaacaccca cgcaacaccg tagaccagaa acgccgtcga
     5701 tagcgcgctg agcacgagat atttcgcgcc cgcttccaaa gttgccgcat ggcgtcgccg
     5761 aaaactgacg aggatgtaaa aggaaatcgt aacgagctcg agcgagacaa agatcattac
     5821 gaagtcgatg gccgagacga gatacatgag cccggcgcaa gtgaagagcg ggagcgcgaa
     5881 aaattccgcg agcaccggtc gaccgcttgc ttcatcctgc acaattgggg cgtagtcgag
     5941 catcataagc aggacgacga tgctcgtaat cagcgcgaac cgtttaaaga agatcgacaa
     6001 tgcatccgcg gtatagaaac tccaaaaccc ggtcgagctt tctgggggcg gtgctgggtt
     6061 gacaaaccac gtcgcaatca acacagccgt gagcgaaccg acggcgatgt aagcgagtgt
     6121 gcgtttttcg agccgcgcag caaaggcttc caacatcaga atcgccattc cgaaaaggag
     6181 cacggcgatt tcgagggcag gcgcgggaat catcgggtcg cgctgacgta gagttgcaat
     6241 gccggcgcga gcaaccgaac aaacgtttgc gggaagaagc cgaaccagag cagtgccgcc
     6301 accagcaaag ccaccggcac gcgcaggctg cggtgaagat cgacaacttc cttccagcgc
     6361 tccggcagcg tgcccatgaa aattcgtcgg taagcgcgca gcatatagac ggtcgaaatg
     6421 accacgcccc acagggcaag gacggtcgcg atttggaaga tgtgaaaccg ctgcacttcc
     6481 caaccggttc ggaacgcgcc gaagaagacc atcacttccg cggcaaaatt cgcgaagccg
     6541 ggcagcccga ccgcggcgaa tgcggcaaga ccaaacgcaa gtccggcaaa gggcatcacc
     6601 ttcgccagac cgccgaggtc ggtgaactca agactcgcag tgcgctggcg cagttcaccg
     6661 gccaaagcaa acagcagcgc aatcgcgagt ccgtgcgcga aaatcaaaat cgccgcgccg
     6721 gtcgtgccga gaacgtttgc gctggcgatc ccgagaaaga tgtagcccat gtgcatcacg
     6781 ctcgagtagc cgagcatcca atcgagccgc ttctgcgcga ttgtgaccag cccgatgtaa
     6841 acgatgtttc cgagcaggag gacgatgagc aggttcgtcc aataacgagc gccttcgggc
     6901 aggagcggca gggccaatcg caacaaacca tagagcccga attttttgag gacaccagcg
     6961 tgcaacatcg ccgccggtgc aggcgcggcg gcatatgctt ccggcgccca ggtgtgaaat
     7021 ggaaagagcg aaatgagaat tccgaaaccg accaacagca gcggataaat gtgccgctgt
     7081 gcatcagccg aaatctggcc agcggcggcg gcgccttgga gcgcgcgaat gtcgaagctg
     7141 cgcgagccga tcgggacgct ctggtaaagc atgatcaaac cgatgagcaa aatgaagctg
     7201 ccaaatgcca gataaatcgt gatcttccat gcggcagcga cgcgattgcc cgtgccccag
     7261 atcccgatca acaagaatgt ggggatcagc gcgagctcgt gaaaggcgta gaagaaaaat
     7321 aaatctagtg acgcgaacgc gccgattgcg cccgccgaaa tgagcaagag gcagccgtaa
     7381 aacacatttt catacctgtc gatctggccg gcgaaccaaa cggctgccac agtgacgatc
     7441 gaggcgagca aaatcatgac cagactcaac ccatcgatac cgatcgtgaa gctcagaccc
     7501 cattctgcgg aaatcgggag gcttacaacg ttttgaaaac cgcgcggcgc gctggggaac
     7561 gtcgcgaata ccgccagcgt tgccacgagg ctgaacgctg atgcgcttaa cgcggtctta
     7621 cgtgccggcg cacccgcaaa gatgagcgta gcggcgacga tcggagcaaa aagaaccaag
     7681 agcagcatca gcggaaaacg gcgaagtaga tgagggcgac aatgccgagg ccgaaaagaa
     7741 acgaataagc ctgcaagttg cccacctgaa acaggcgaag cagcgtgccg agtccccagg
     7801 tgccgctgct cgcgccacgc actgctgcgg catcgatgag ccagcggtca gtgaaagccg
     7861 acacgcgcgc gagaagttcc tgcgtcgag
//
LOCUS       DASTDG010000096         5484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_482056, whole genome shotgun sequence.
ACCESSION   DASTDG010000096 DASTDG010000000
VERSION     DASTDG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482056"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VFO30_03060"
     CDS             <1..2260
                     /locus_tag="VFO30_03060"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET9418295.1"
                     /translation="AVQNVFVNLYNKGLIYRGRRMINWDPAAQTALSDEEVINKPQKG
                     SLYYVRYEIVEERSASAPLATQEAQAGGLRAFIEVATTRPETIMADTGVAVHPRDNRY
                     ADLVGKHAWRPLARDKIPIIADDAIDPEFGTGALKVTPAHDKLDFEIGQRHNLPVVDV
                     LTADGRINCLAEPELHGLDRFAARERAAELLQDRHLLAKSEPYENNVGFSDRSDVPIE
                     PSISEQWFLHYPRTNEALAIVRDHLITFFPAYWEKVYGQWLENIQDWCISRQVWWGHR
                     IPAWYWRDKSEIRNPKSEIYVGLEPPANPEKWRQDEDTLDTWFSSWLWAYETMDEETR
                     RKFYPTSVLVTGPDIIFFWVARMIIAGLEFKPGKSTRDEDNIPFRHVYFTGIIRDKLG
                     RKMSKSLGNSPDPLELIDKYGADGLRFGLMRIAPSGQDIWFDEKQIEQGRNFATKLWN
                     VARFRQMHGATLISPIEKLDGFKPSSFGSMSIYAIEVLSRLNETIEAVEQGYREYAFN
                     SVAQRLYDFVWSDYCDWFVEAAKTGIFGADEAKKNSALSVMDYVLSAVLRLLHPFMPH
                     ITEELWSLFGFGDGSIQFAEPPKPIALDELDVATARTRVSAIYSAVQAGRNLRAEAKI
                     PSNKKARFVLRATDGDVSRESGTLERLLNAEQLTLDRQYQGVPGEPVAVTPLGEIFLT
                     VGESDKEAERERLDREIERLETELRAAEEKLANASFVERAPATVVDEHRRRQKDISEQ
                     VAKLKQARLRLQ"
     gene            complement(2302..3048)
                     /locus_tag="VFO30_03065"
     CDS             complement(2302..3048)
                     /locus_tag="VFO30_03065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9418296.1"
                     /translation="MRFSQKMAKLANGRAFSSAGNRIRRFLVNWRLPVKLEPQAIIDR
                     IDRRKFEEIYARYVVDDPGENWPKYLDLPRWIDININRIRAVGLDIPARKRVLDLGCG
                     AGYFAYVAQLLGHDMVGLDIDQLPMFAESTRMLGVRRVIWRVRPFVPLPELGEKFDVV
                     TAFMICFNNHKMPDLWGVPEWEFFLDDLAHHLKPRSRVWLEFNLEWDGTFYTPELRDY
                     FESRGAKTDNQKVIFTSGVLAPLSASPVAR"
     gene            3110..5155
                     /locus_tag="VFO30_03070"
     CDS             3110..5155
                     /locus_tag="VFO30_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET9418297.1"
                     /translation="MSWEKKLLWVAITLAGAGALAVLALSRGEQVSALWIIVAGLCAT
                     AISYRFYSKWLATKVLVLNDDRATPALVKNDGKDFVPTNRWMVFGHHFAAIAGPGPLV
                     GPVLAAQFGFLPGTLWILIGATLGGAVHDMIVLFASVRRGGKTLGQMVKEEIGPVVGV
                     LALVSVLAIMIILLAVLALVVVQALAQSPWGVFTIGMTIPIALIMGAGLRSGKATVAA
                     VTIFGVGGLLLAVWGGQFLAHFPAVETWFRHDAKWLAWAIMLYGLAASILPVWMLLTP
                     RDYLSTFMKLGTVGALAAAVILIHPTLQMPALTRFIDGSGLVFAGPVFPFVCITIACG
                     AVSGFHSLIASGTTPKMLGRESRIRDIGYGAMVTEMMVALMAIIAASVLNPGEYFAIN
                     MKGTPSQVVATVSAAGFPVTENEMSILAHNLGETTMFNRAGGAPTFAVGMAHMFARVS
                     ASPTAMALWYHFAIMFEALFILTTIDAGTRVGRFLLQDLLGNVWRPLGNTRSWTANLV
                     ASTLLVAAWGWFLYQGVIDPLGGVNTLWPLFGLANQLLSVIALCLGTTVLIKMHKAKY
                     LFVTLVPLCFMCAVTFSAGYLKIFSADPKLGFLSGAGSLVQQAGGLANPHKASELIRQ
                     AGVWRFDALVACTFLALVFLIVLGSAIHWLQLVRGTKAIKLNEAEFVPIASVQVANI"
     gene            complement(5240..>5484)
                     /locus_tag="VFO30_03075"
     CDS             complement(5240..>5484)
                     /locus_tag="VFO30_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418298.1"
                     /translation="DSIAFTFVSDEFNGVTRDHNGNVRPYAPRSFTSLSQAEEENGQS
                     RIYLGIHWSFDKTQGIAQGRRVADYVFDHAFTPLDH"
BASE COUNT         1117 a         1510 c         1719 g         1138 t
ORIGIN      
        1 cgccgtccag aacgtatttg ttaatctgta caacaaaggc ctgatctacc gcggccggcg
       61 gatgatcaac tgggacccgg ctgcgcaaac cgcgctctcg gacgaggagg tgatcaacaa
      121 accgcagaaa ggaagtctct actatgtccg gtacgaaatc gtcgaggaaa gaagcgcaag
      181 cgccccgctt gcgactcaag aagcgcaagc cggaggctta cgcgccttta tcgaagtcgc
      241 gacgacgcgg ccggagacga tcatggcgga taccggtgtc gctgttcatc cacgcgacaa
      301 ccgatatgcc gatctcgtcg gcaaacacgc ttggcgaccg ctggcgcgcg ataaaatccc
      361 gatcatcgcg gatgacgcga tcgatccgga gttcggtact ggagcgctaa aagtgacgcc
      421 tgcgcacgac aaactcgatt tcgagattgg tcagcgccat aacctgccgg tcgtcgacgt
      481 tttgaccgcc gatgggcgaa tcaattgcct ggccgagccc gaactgcacg gcttggaccg
      541 atttgcagcg cgggaacgcg ccgccgagct actgcaagac cgacatctgc tcgccaaaag
      601 cgagccgtac gagaacaacg tcggcttcag cgaccgcagc gacgtgccca tcgagccaag
      661 catcagtgaa caatggtttt tgcattaccc aagaactaac gaggcgctcg ccatcgtgcg
      721 cgatcatctc atcacgttct tccctgcgta ctgggagaaa gtttacggtc aatggctcga
      781 gaacattcag gactggtgca tcagccggca ggtttggtgg ggacatcgaa taccggcgtg
      841 gtactggagg gacaaatccg aaatccgaaa tccgaaatcc gaaatttatg tcggcctcga
      901 gccgccggct aacccggaga aatggcgaca ggatgaggac acacttgata cgtggttttc
      961 gtcctggctg tgggcgtacg agacgatgga tgaggagacg cggcggaaat tttatccgac
     1021 cagcgtgctc gtgaccggac cggacataat tttcttctgg gtggcgcgca tgatcattgc
     1081 cgggctggag ttcaagccgg gtaaatcgac gcgcgatgaa gacaatattc cgttccggca
     1141 tgtttatttc accgggatta ttcgcgacaa actcgggcgc aagatgtcga agtcgctagg
     1201 caactcgcct gatcctctcg agttgatcga caagtacggc gcggacggat tgcggttcgg
     1261 gctcatgcgg attgcgccga gtggtcagga catctggttt gacgaaaaac agatcgaaca
     1321 aggccgcaac ttcgcgacca agctttggaa cgtggcgcgc ttccgccaaa tgcacggcgc
     1381 aacactaatt tcgcccatcg aaaaacttga cggctttaag ccgtcaagtt ttggaagcat
     1441 gtcgatctat gcgatcgagg ttttgtcgcg cttgaacgaa acgatcgaag cggtcgagca
     1501 gggataccgc gagtacgcgt tcaactcggt cgcgcagcgc ctctacgatt tcgtctggag
     1561 cgactattgc gattggttcg tcgaagcggc aaagaccggc attttcggag cggatgaggc
     1621 aaaaaagaac tccgcgctct cggtgatgga ttacgtgttg tcggccgtgt tacggttact
     1681 gcatccgttc atgccgcaca tcacggaaga gctgtggtcg ttgttcgggt ttggtgacgg
     1741 ctcgatccaa ttcgccgagc cgccgaaacc aatcgcgctg gacgagctcg atgttgcaac
     1801 cgcacgaaca cgcgtgtcgg cgatctactc ggctgtgcag gccgggcgga acctccgtgc
     1861 ggaagcgaag atcccgtcga acaaaaaggc gcggttcgtt ttgcgcgcga ctgatggtga
     1921 cgtgagtcgc gaatcgggca cgctcgagcg gctgctcaat gccgagcagc taacgttaga
     1981 tcgacaatac cagggcgtgc cgggcgagcc ggtggcggtg acgccgttgg gcgagatctt
     2041 tctcaccgtc ggcgagtccg acaaagaagc tgagcgcgag cggctagatc gtgagatcga
     2101 gcgactcgaa accgagctgc gtgcagcaga ggaaaagctc gcgaacgcgt cctttgtcga
     2161 acgcgcgccg gcgaccgttg tagacgaaca tcggcgacgg caaaaagaca tcagcgagca
     2221 agtggcaaag ctgaagcagg cgcgcctacg cctgcaatga aagttgccac aagaagcaaa
     2281 gaagccctgc gacgcgagct gttaccgagc aactggcgaa gctgaaagag gcgcgagaac
     2341 gcctgaagta aagatcacct tctgattgtc ggtcttggcg ccgcgactct caaaataatc
     2401 gcgcagctcg ggcgtgtaga aagtgccgtc ccattccaga ttgaactcga gccaaacgcg
     2461 gctgcgcggc ttgaggtggt gcgcgaggtc gtccagaaag aattcccatt ccggcacgcc
     2521 ccagagatcg ggcattttgt ggttattgaa acagatcatg aacgccgtca cgacatcgaa
     2581 tttttcgccg agctcgggca gcggaacgaa cggacgcaca cgccagatga ctcggcgcac
     2641 gcctaacatg cgggtgctct ctgcgaacat cgggagctgg tcgatatcga gcccgaccat
     2701 gtcgtggcct aacaactgcg cgacgtaagc gaagtagccg gcgccgcacc cgagatcgag
     2761 cacgcgtttg cgcgcgggga tgtcgagacc gacggcgcga atgcggttaa tgttaatgtc
     2821 gatccagcgg ggcagatcga gatacttcgg ccagttctcg cccgggtcgt caacgacgta
     2881 acgcgcgtaa atctcttcga attttcggcg gtcgatcctg tcgatgatcg cctgaggctc
     2941 aagtttcacc ggtaatcgcc agtttacgag gaaacgacga atgcggtttc cggcgctgct
     3001 gaacgcgcgg ccgttcgcca gttttgccat tttctgcgag aagcgcattt gatgtgacga
     3061 tactttgcag cggtggcgtg ccgcggcaat ttcaattaag ctcaaaccga tgagctggga
     3121 aaaaaagctg ctctgggttg cgattaccct tgctggagca ggtgcgctcg cggtgctggc
     3181 gctttcgcgc ggtgaacaag tcagcgcgct ttggatcatc gtcgctggtc tgtgcgcgac
     3241 cgcgatcagc taccggttct acagcaaatg gctggcgaca aaggtgctgg tgttgaatga
     3301 tgaccgggca acgccggcgc tagtcaaaaa cgacggcaaa gatttcgtgc cgaccaatcg
     3361 ctggatggtg ttcggccatc attttgcggc gattgcaggt ccggggcctt tggtcgggcc
     3421 ggtgctcgcg gcgcaattcg gatttttgcc gggaacgctc tggattttga tcggcgcaac
     3481 tcttggcggc gccgtgcacg acatgatcgt gctgttcgcg tcggtgcgtc gcggcggcaa
     3541 aacgcttggg caaatggtga aagaagaaat cggtcccgtg gttggcgtgc tggcgttggt
     3601 gagcgtgctc gcgatcatga tcattctgct cgccgtcctc gcgctcgtcg tggtccaggc
     3661 gctggcgcaa agtccttggg gcgtgttcac gattggaatg accattccaa ttgcgctgat
     3721 catgggcgca gggctgcgca gcgggaaagc gaccgtcgcc gcagtgacga tcttcggtgt
     3781 aggcggtctg ttgctcgccg tctggggtgg acaatttctg gcgcatttcc ccgcggttga
     3841 aacttggttc cggcacgatg cgaaatggct ggcgtgggcg atcatgcttt acggattggc
     3901 cgcgtcgatt ttgccggtct ggatgctgct gacgccgcgc gattatctga gcacattcat
     3961 gaaactcgga actgtcgggg cgctcgcggc cgcggtgatc ctgattcacc cgacgctgca
     4021 aatgccggcg ttgacgcggt tcatcgatgg cagcggtctg gtgtttgccg ggccggtttt
     4081 cccctttgtt tgcatcacga tcgcttgcgg cgcggtgtcc ggctttcact cgctcatcgc
     4141 gtccggcacc acgccgaaaa tgctcggccg tgaatcacgt atccgggata tcggctacgg
     4201 cgcgatggtg acggagatga tggtggcgct gatggcgatc attgccgcgt cggttctgaa
     4261 tccaggcgaa tacttcgcga tcaatatgaa aggcacgccg agccaggtcg tagcgacggt
     4321 gtcagcggcg ggatttccgg tgaccgaaaa tgagatgtcg atcctggcgc acaatttggg
     4381 tgagaccacg atgttcaatc gcgccggcgg cgcgccgacg ttcgctgtcg gcatggccca
     4441 catgttcgcg cgcgtctcgg ccagcccgac agcgatggcg ctctggtatc acttcgccat
     4501 catgttcgag gcgctgttta tcctgacgac gattgatgcc ggcacacgtg tcgggcgatt
     4561 tctcctgcag gacttgttag gcaacgtgtg gcgccccctc ggcaatacgc gctcatggac
     4621 agcaaacctt gtcgcgagca cgctgctcgt cgcggcgtgg ggctggtttt tgtatcaagg
     4681 cgtgatcgat ccgctcggcg gcgtaaacac gttgtggccg ctcttcggct tggcgaacca
     4741 gcttctctct gtcatcgcgc tctgcctcgg caccaccgtg ctcatcaaga tgcacaaagc
     4801 caaatacctg ttcgtgacgc tggtaccgct ctgtttcatg tgcgcggtga cgttctcggc
     4861 tggttacctg aaaatttttt cggccgatcc aaagctcggt tttctcagcg gtgcgggttc
     4921 actcgtgcaa caagcgggcg gcctggctaa cccgcacaaa gcgagcgagc tcatccggca
     4981 ggccggcgta tggcgcttcg acgcgctcgt cgcatgtacc ttcctcgcgc tcgtgtttct
     5041 gatcgtgctc ggctcggcga ttcactggtt gcagctcgtg cgcgggacaa aggcaatcaa
     5101 actgaacgag gcggagtttg ttccgatcgc gagtgtgcag gtggcgaaca tctagcggtg
     5161 agcgatggca ggcgggccgc gttcgctgcg gctacgtcgc gccaggaagc gcttgcctac
     5221 aaattcagca accgccgcgt cagtgatcca gcggcgtgaa ggcgtggtcg aagacgtaat
     5281 cggcgacgcg gcggccttgg gcgatgcctt gggttttgtc gaatgaccaa tggatgccga
     5341 gataaatgcg gctttgaccg ttttcctctt cagcttgcga taacgaggtg aagcttcgcg
     5401 gcgcgtacgg ccgaacattc ccgttgtggt cgcgagtcac cccgttgaat tcgtcggaga
     5461 cgaaggtgaa cgcgatcgaa tcgg
//
LOCUS       DASTDG010000097         6887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_287889, whole genome shotgun sequence.
ACCESSION   DASTDG010000097 DASTDG010000000
VERSION     DASTDG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287889"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFO30_03080"
     CDS             complement(<1..430)
                     /locus_tag="VFO30_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418299.1"
                     /translation="MKPAVWRILFATTTTAVVLRAQEAAPPNMDVAPRNTARPMPSPT
                     ASVAPTVPPNLPEVSQLDEVFKQSSLGGKADEFRLHAEWRRLRNQIANAPDVVAAKRA
                     ADAARTDLERRQLLRRYYEICYAHMQALAREPDVKAGLEEM"
     gene            complement(450..523)
                     /locus_tag="VFO30_03085"
     tRNA            complement(450..523)
                     /locus_tag="VFO30_03085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Pro,seq:tgg)
     gene            complement(543..4292)
                     /gene="metH"
                     /locus_tag="VFO30_03090"
     CDS             complement(543..4292)
                     /gene="metH"
                     /locus_tag="VFO30_03090"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9418300.1"
                     /translation="MSPKVHPLELLIRERVVVLDGAMGTMIQRRKLSEADYRGERFRD
                     WKGKDLKGSLELLLLTKPDVVEQIHQQYLEAGADIIETNTFSATTIGLHDFLFQNGPV
                     SGRKDAEFFQRVVDDVDLRALVHEMNLAGATIARRAADRVAHDTGTNRFVAGSLGPLP
                     VTGSISPDVNDPAFRAVTFDQLRQTYFDQARALLEGNVDLLVVETVFDTLNAKAALFA
                     IVQVFEQTGRKVPLILSGTVADRSGRTLSGQTVEAFLISIAHAQPLVVGLNCALGPDE
                     MSAYIEELAHISPYYLSAYPNAGLPDPLSETGFPETPESFAPKVVNWARNGWLNIVGG
                     CCGTTPAHIRLIANAVRDCPPRQTFSMRRSASLQLSGLEPLNITPEMGFVVIGERTNI
                     TGSPKFSKLILADDFDGGLSVARQQVQGGANMLDMNMDEGMIDSEPAMARFLRLVGSE
                     PEIARVPIMIDSSKWSVIEAGLKCVQGKAVVNSISLKNGEEEFLRQGRLVRQYGAAVI
                     VMAFDEQGQADNFQRKIDICARAYGLLTERAGFPASDIIFDPNVLTVATGLEEHRNYA
                     VDFIEATGWIKQNLSGARVSGGISNISFSFRGNNAVREAMHAAFLYHAIRAGLDMGIV
                     NAGQLAVYEEIEPELRERVEDVLLNRRADATERLVEFAERVKTKGKVQIKDEVWRSQP
                     VEERLKHALIHGIVDHIDIDTEEARQKSKRPLDVIEGPLMAGMSVVGDLFGAGKMFLP
                     QVVKSARVMKKAVAYLMPFMEAEKLAAAKPQGRIVMATVKGDVHDIGKNIVGVVLQCN
                     NYDVIDLGVMVPAPKILETAREKNADAIGLSGLITPSLDEMVHVAQEMEREKFEVPLL
                     IGGATTSRAHTAVKIAPQYHGSTVHVLDASRAVGVVSSLLNVEARAHFDSKTRDDYAR
                     LREQHAKKSSEKKLLTLAQARENRTAIDWSDYAPPQPEFTGARVVCPPIVDLREFIDW
                     SPFFHVWELRGRYPAIFDDPSVGKQARELFADAQGILDQITADNSLRARGVFGFWPAN
                     SNRDDVDLFTDETRLAKLGTFYFLRQQMQKPAGQFNHSLADYIAPNGQDYIGGFAVGI
                     HCADELAKKFAAAHDDYSAIMAKALADRLAEAFAEYLHKQARIAWGFGRNENLSNEDL
                     IREKYRGIRPAPGYPACPDHVEKGMLFDLLNAEHKTNIHLTESFAMHPAASVSGYYFS
                     HPKAKYFGVGQIRRDQVEDYARRRGETTAAVEKRLAANLGYEPNP"
     gene            4527..5327
                     /locus_tag="VFO30_03095"
     CDS             4527..5327
                     /locus_tag="VFO30_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9418301.1"
                     /translation="MNRNGRSGVNLRERRRTNAHLSSGDYGGTAIRSPSKKDNVRSKS
                     GLAVSRVGIVVVDDHRFMRELIAAMLRRHDSRYEVVAEVADAKSAIDACQRLSPDLVV
                     LDINLPDASGIDIVPQIKKVSPHTRVLLCTAYVTDDRIVDALTSGAEGFAEKTNTWRD
                     FAEAIDRVLGGEHYFCARPSAAFANAAPAIGKGLAPASTAGLSPREKEILALIAEGAT
                     SKEIASKLGISVATVETHRRRLMAKLHIRNVAGLVVFAFRAGLIRLQR"
     gene            5595..>6887
                     /locus_tag="VFO30_03100"
     CDS             5595..>6887
                     /locus_tag="VFO30_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9418302.1"
                     /translation="MSKARMICWCGKYHKAFVTVFAALASLAIPTLIDASSSKQVAQL
                     APLVVPRTGHVSTVLADGRVLITGGRDNGGNIVAVSEIFDPTTETSTASAVLTIPRAE
                     HTATLLPDGRVLVAGGTGTTGSLASAEVFDPTKAAAGFRLLSATMSAARSGHTATLLN
                     NGTVLIAGGDADGTAEIFDPATEVFTSTLWNLAVPRTGHTATLFSDDSVLLAGGNTDS
                     MELFTAADQKFTLESRKMSAVRTGHWALELSDTRLLLFEGDTGHTIDAFNPSADTLTL
                     KDSLDTPASSATLLANGKILVLGAVVAGLYDPNAVAPTPAFTAFDEVSIPDSTLLLRT
                     GETATELSRDKKVFIAGGVDAQKLFGVPALFNPAKIWTDKDDYLPDEPVLLYGSGWKA
                     NEDVYLYAVDSETEQWTYGSTAHADTNGAFAVEPYFIVQ"
BASE COUNT         1387 a         2083 c         1935 g         1482 t
ORIGIN      
        1 tcatctcttc caagccagct ttcacgtccg gctcacgagc gagcgcctgc atgtgcgcgt
       61 agcaaatctc gtaatagcgc cgcagcaatt gccgcctctc caaatcggtt cgcgccgcat
      121 ccgctgccct ttttgccgcc accacgtccg gtgcgttcgc aatttgatta cgaagtcgtc
      181 gccattcggc gtgcaagcgg aattcatcgg cttttccgcc taacgaactc tgcttgaaga
      241 cttcgtccaa ctgcgatact tccggcaggt tcggcggaac ggtcggcgcc acacttgccg
      301 tcgggctggg catcggtcgt gccgtgttcc gcggcgcaac atccatgttt ggtggcgctg
      361 cctcctgcgc gcgcaatacg acggctgtcg ttgtcgtggc aaaaagaatt cgccaaaccg
      421 ccggtttcat gcgcaatgct gaactaaagt cggggcgaga ggatttgaac ctccgacctc
      481 ctggtcccaa accaggcgct ctaccaagct aagctacgcc ccgcgattgc gcgtgatgct
      541 tatcatggat tcggctcata gccaaggttc gccgcgagcc gcttttcgac cgcggccgtt
      601 gtttcaccgc ggcgccgcgc atagtcttca acttggtcgc gcctgatttg ccccacgccg
      661 aagtattttg cctttgggtg tgaaaaataa tatccactca cgctcgccgc gggatgcatc
      721 gcgaaggact cggtcaggtg gatgttggtc ttgtgttcgg cattcagcaa atcaaacagc
      781 atgccttttt ccacgtgatc gggacacgcc ggataacccg gcgccggacg aatgccgcgg
      841 tatttttcac gaatgagatc ctcattcgac aaattctcgt tgcgtccaaa tccccacgcg
      901 atcctcgcct gcttatgcaa gtactcagcg aatgcttcgg caagacggtc ggccaatgct
      961 ttagccataa tcgcgctgta atcgtcgtgc gctgcggcaa atttcttcgc cagctcgtcg
     1021 gcgcaatgaa tgcccacggc gaaaccgccg atgtaatcct gcccattcgg agcgatgtaa
     1081 tcggccagac tatgattgaa ttgccccgcc ggtttttgca tctgctgccg cagaaagtaa
     1141 aaggtaccaa gtttggcgag ccgcgtttca tcggtgaaaa gatcgacatc atccctgttc
     1201 gagttcgccg gccaaaatcc aaacactccg cgcgcgcgga gcgaattatc ggccgtgatc
     1261 tgatcgagaa tgccctgcgc atccgcaaat agttcccgcg cctgtttgcc gacgctgggg
     1321 tcgtcaaaaa ttgccggata acggccgcgc aattcccaaa catggaagaa tggcgaccaa
     1381 tcgatgaatt cccgaagatc gacaattggc gggcatacaa cgcgcgcgcc ggtaaattcg
     1441 ggttgcggcg gcgcgtaatc gctccagtcg atcgccgtcc gattttcgcg agcctgggcg
     1501 agcgtcagca attttttttc gctgctcttc ttggcgtgct gctcccgcaa acgcgcgtaa
     1561 tcgtcgcgcg ttttcgagtc aaagtgggcc cgcgcctcga cgttcaatag cgagctcacc
     1621 acgcccactg cgcgcgaggc gtcgagcacg tggacggtgc tgccatgata ctgcggcgcg
     1681 attttcaccg ccgtatgcgc acggcttgtc gtggcgcccc caattaacaa cggcacctcg
     1741 aatttctccc gctccatttc ctgcgcgacg tgcaccatct cgtcgagtga gggcgtaatc
     1801 agcccgctca gtccgattgc gtccgcgttt ttttctcgcg cggtctccag aattttcggt
     1861 gccggaacca tcacaccgag gtcgatgacg tcgtaattgt tacattgcag cacgacgccg
     1921 acgatgttct tgccgatatc gtgcacgtca ccttttaccg tcgccatgac gatgcggcct
     1981 tgcggttttg cggctgccag tttctcggct tccatgaacg gcatcagata ggcgacggct
     2041 tttttcatca cccgtgcgct cttcacgacc tgcggcaaaa acatcttgcc cgcgccaaac
     2101 aaatcgccga ccacgctcat gccggccatg agcggacctt caatcacatc gagcggccgc
     2161 ttcgatttct gccgcgcctc ttccgtatcg atgtcgatgt gatcgacgat tccgtggatg
     2221 agtgcgtgct tgagccgctc ttcgactggc tggctgcgcc agacttcatc tttgatttga
     2281 accttgcctt ttgttttgac gcgctcggca aattcgacta gccgctcggt cgcgtccgcg
     2341 cgacggttga gcagcacgtc ctcgacccgc tcgcgcaatt ccggttcgat ctcctcatag
     2401 accgcgagct gtcccgcgtt gacgattccc atgtcgagac cggcgcggat cgcgtgatac
     2461 agaaacgcgg cgtgcatcgc ctcgcgcacc gcgttgttgc cacgaaatga gaaggaaatg
     2521 ttgctgatac cgccgctgac ccgcgcgccg gacaagttct gtttgatcca gcccgtcgct
     2581 tcgatgaaat cgacggcgta gttgcgatgt tcttcgagac cggtggcgac ggtcagcacg
     2641 ttcgggtcga agatgatgtc gctcgccgga aaaccggcac gctcggtcag taatccgtaa
     2701 gcgcgcgcgc agatgtcgat cttccgctgg aaattatcgg cctgcccctg ttcatcgaac
     2761 gccatgacga tgacggcggc gccgtattgc ctaacgagtc ggccctggcg cagaaattcc
     2821 tcttcgccat tttttaagct gatcgaattg acgaccgctt ttccctggac gcatttcagc
     2881 ccggcttcga tgacgctcca cttcgagctg tcgatcatga tcggcacgcg cgcgatctcc
     2941 ggctcgcttc cgacgagtcg caggaagcgc gccattgcgg gttccgagtc gatcattcct
     3001 tcgtccatgt tcatgtcgag catgttcgcg ccgccctgca cctgctggcg cgcaacggac
     3061 aggccgccgt cgaaatcatc cgccaggatc agcttcgaga atttcggcga accggtgatg
     3121 ttcgttcgct cgccaataac tacaaaaccc atttcgggcg tgatgttcag cggctcgagc
     3181 ccggagagtt gcagggacgc actccgtcgc atcgaaaacg tctggcgcgg cggacaatcg
     3241 cgcactgcgt ttgcgatcaa ccggatgtgc gccggcgttg tgccgcaaca gccaccgacg
     3301 atattgagcc agccattgcg cgcccaattc accacttttg gcgcgaacga ttccggcgtt
     3361 tccgggaacc cggtttcaga cagcgggtcc ggcagacctg cgttcgggta cgcactcaga
     3421 tagtagggag aaatgtgcgc gagctcctcg atataagccg acatctcgtc cggtccgagc
     3481 gcgcaattca agcccacgac gagtggctga gcgtgcgcga ttgaaatgag aaacgcttcg
     3541 acagtttggc cgctgagcgt ccgtccagaa cggtcagcaa ccgtgcccga gaggatgagt
     3601 gggactttgc gtccggtctg ctcgaacacc tgcacaatcg cgaagagcgc cgccttggcg
     3661 ttcaatgtgt cgaaaaccgt ttcgacaact agaagatcga catttccttc cagcagtgcc
     3721 ctcgcctggt cgaagtaggt ttggcgcaac tggtcgaaag tgacggcccg aaatgccggg
     3781 tcatttacat cgggcgaaat tgaacctgtg acgggaagcg gcccgagcga tcccgcaaca
     3841 aatcggttcg tgccggtgtc gtgcgcgacg cggtctgccg cgcgccgggc aattgtcgcg
     3901 ccggcaaggt tcatttcgtg gacaagcgcg cgcagatcga cgtcatcgac aacgcgctgg
     3961 aaaaattccg cgtcttttcg tccgctcacc ggcccgtttt ggaagagaaa atcgtgcaga
     4021 ccgatggtcg tcgcgctgaa ggtgttcgtt tcgataatgt cggctccagc ttcgagatac
     4081 tgctgatgaa tctgctcgac cacgtcgggt ttggtcagga gaagaagctc gagactgcct
     4141 ttgaggtctt tgcctttcca atcgcggaag cgctcgccgc ggtaatccgc ttcgctcagc
     4201 ttgcggcgct ggatcatcgt gcccattgcg ccgtcgagta cgacaacgcg ctcgcgtata
     4261 agcaactcga gtggatggac cttaggcgac attatggctg ggaactaagc ggtgttcgtg
     4321 ccgctgatca agaaacaaat taacgcatca tgatacgtag atgtgttgtc atctccaaat
     4381 ttgcatgaag tggcctccgt gcccccaacg gcctatagga ctttaaccga cgttgcggca
     4441 atgcatcgtc ctgataacaa ctgattgcac gaggacaacc acttttgtta gactgggact
     4501 tgtcgcccaa gggagaaatg gctcatatga accgtaacgg caggtcgggc gtcaacttgc
     4561 gcgagcggcg gcgtacaaat gcgcatttaa gctccggcga ctacggcgga acggcgattc
     4621 gctcgccgag caaaaaggac aacgtgcgaa gcaaaagcgg gctcgccgtg tcacgtgtag
     4681 gcatcgtcgt tgtcgatgat catcgcttca tgcgtgaact gatcgctgcg atgttgcgcc
     4741 gccatgacag ccgctacgag gttgtggccg aagtcgccga cgccaaaagc gccatcgatg
     4801 cctgccaaag actttccccg gatttggtcg tgctcgatat caatctgcca gatgcgagcg
     4861 gcatcgacat cgtcccgcag attaaaaagg tctcgccgca cacacgcgtg ctgttgtgca
     4921 ccgcctatgt gacagatgac cggatcgtgg atgcactgac cagcggcgcg gaaggatttg
     4981 ccgagaagac aaacacctgg cgggacttcg ccgaagcgat tgatcgcgtc ctcggcggtg
     5041 agcattattt ctgcgcccgc cccagcgccg cattcgcgaa cgcggcgcca gctatcggca
     5101 aaggtctcgc gcctgcgagc acggcaggtc tcagcccgcg tgaaaaggaa atcctggcgc
     5161 taatcgcgga aggggcgacg agcaaagaaa tcgcgtccaa gctcggcatt agcgttgcga
     5221 ccgtagaaac tcaccgccga cgtctgatgg cgaagctgca cattcgaaat gtcgcagggc
     5281 tggtggtgtt cgcgttccga gcggggctga tcaggctgca gcgctagagg gacagccagg
     5341 cggacgtgca cttattcgcg tccgtcgcgg cccgtcgcgt ctcaaaagac catgagacct
     5401 gaacccctac aggttttcca tgattactaa aatacagggt tttgagatct accgttatcg
     5461 tctacgcgcc gctacacttg tcggcccgtg atctccgcaa agaagccctt cgcgcgattg
     5521 tctgcacggc agcgctcgca ctgtccagcg gcagaacgcc ttccacaagt cgccaatcag
     5581 ttagcgacta ctaaatgagc aaagcgcgaa tgatctgctg gtgcggaaaa taccacaaag
     5641 cattcgttac ggttttcgcc gccctcgcaa gcttggcgat accgacattg atcgacgcct
     5701 caagttctaa gcaggtcgcg cagctcgctc cgcttgttgt gccgcgcaca ggacatgtct
     5761 ccaccgtgct cgccgatggc cgcgtcctga tcaccggtgg acgcgataac ggcgggaaca
     5821 ttgtcgcagt ctcagaaatc tttgatccta cgactgaaac gtcgaccgcg agcgccgtac
     5881 tcactattcc ccgcgccgag cacactgcaa cgctcctgcc cgacggccga gtactcgttg
     5941 ccggcggtac cggcacgacc ggatctctgg cgtccgcaga agtctttgac ccgacaaagg
     6001 cagccgctgg gttccgtttg ctgtctgcaa ctatgagcgc cgcgcgcagc gggcacaccg
     6061 ccaccctgct taataatggc acggtactca tcgccggcgg tgatgctgac ggcacggctg
     6121 aaatattcga cccggcgaca gaagtgttta cctctacgct ttggaatttg gccgtgccgc
     6181 ggaccggtca taccgccact ttgttttccg atgacagcgt tttgctcgcg ggtggcaaca
     6241 cggattcgat ggaactattc acggccgcag atcagaaatt tacgctcgag tcgcgcaaga
     6301 tgtcggcggt acgcaccggc cattgggcgc tcgagctcag tgacacgcgt ctgctcttgt
     6361 tcgagggaga cacaggacac acgattgacg cgttcaatcc gtcggccgac acgctcaccc
     6421 tcaaagacag cttggacacg cctgcatcga gcgcgacgct tctggccaac ggaaaaattc
     6481 tcgtgctcgg ggcggtggtg gctggtcttt atgatccgaa cgccgtcgct ccgaccccgg
     6541 ccttcaccgc gttcgatgaa gtatcgattc ctgacagtac tctactgcta cgtaccggcg
     6601 agaccgccac agagctttcg cgcgacaaga aagttttcat cgccggcggc gtggacgcgc
     6661 aaaagctttt cggcgtccct gccttgttta acccggcgaa aatttggacg gacaaggatg
     6721 attacctgcc ggatgagccc gtcctactct atggctcagg ctggaaagca aacgaagacg
     6781 tttatctcta cgccgtagat tcagaaacgg agcagtggac ctatggcagc accgctcatg
     6841 ctgacacgaa cggcgcattc gccgttgagc cctatttcat cgttcag
//
LOCUS       DASTDG010000098         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1365920, whole genome shotgun sequence.
ACCESSION   DASTDG010000098 DASTDG010000000
VERSION     DASTDG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365920"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1215
                     /locus_tag="VFO30_03105"
     CDS             229..1215
                     /locus_tag="VFO30_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET9418303.1"
                     /translation="MPLVVTRAGFLTSVQDLGRGGLRQFGVSLSGALDPHALRVANLL
                     VGNDESAAGLEVTFGDLRLRFTGERIAVWCGGDFEARVESTAIPAGHPFLLRTGEELI
                     IDRPRIGCRAWLAISGGMDVPIVLGSRSTDLRGNFGGTDGRALRDGDEIPLARNSERA
                     DILIERLRNQKVANWKPPHAWSSPVQREPLLRFIRGADLHRFNALTLQRFNEEPFIVS
                     PDSDRMGVRFDAPELQREDHVDLVSEAVAPGTVQVPPSGKPILLLGDCQTIGGYPKIG
                     HVITVDLPIAAQLRAGDQVRFREVSLAEAHQLWLDRERDLAQFRQGLEAHFT"
     gene            1212..1970
                     /locus_tag="VFO30_03110"
     CDS             1212..1970
                     /locus_tag="VFO30_03110"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET9418304.1"
                     /translation="MKMIVDLNADLGEGAGHDDELLAIVSSASIACGFHAGDAGTMRR
                     AIETARDYSVAVGAHPSLFDRQNFGRKELAVAPDEVFDAVAYQLGIFQAIAEAAGVRP
                     NHVKPHGALYNMAVRDEALADAIGRATAKMDNALILFAPDGSALARMGEANGLQIARE
                     LFADRNYLDTGWLVPRSRPDALLHDPIEAAERVIRMLREGKVKSVDGVDVDVRGETIC
                     LHGDTPGAVEFARGLRSRLEQEGVTIRAPKSNRL"
     gene            2021..>2542
                     /locus_tag="VFO30_03115"
     CDS             2021..>2542
                     /locus_tag="VFO30_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9418305.1"
                     /translation="MNDKIDNQQPSGLVRALGPIDATMIVIGSMIGSGIFITSAESAR
                     LNGAPGWLLLAWAVAGLLTITGALCCSELATMMPRAGGVYVFFREAYGPTLGFLYGWT
                     LFLVIQTGTIAAVAIAFARFLGVFVDAISPNNYLIAPVQLGNAYAISLSTQQLIAIAL
                     IALLTWTNTRGLKL"
BASE COUNT          488 a          769 c          757 g          528 t
ORIGIN      
        1 gctggaacat catcggtcgc accccgctgc gattgttcga tccgcaaaaa gaccctccgg
       61 cgctactgcg tgccggtaat cgcgtgcgtt tccgcgccat tacgcgcgaa gaattcgatc
      121 gcttaaaatg aggctaccgc cgctggggcg agactctgtc gaggcggcga aacccgagcc
      181 aattcgatgg ctccgcagag cgttacccag cgagccagca ctgaaatcat gccactagtg
      241 gtcacgcgcg ccggtttcct gaccagcgtt caagacttgg gccgcggcgg tcttcgacaa
      301 tttggtgtct cactcagcgg cgctctcgat ccgcacgcct tgcgtgtggc caatttgctc
      361 gtcggtaatg atgaatcggc tgccggactt gaagtgacgt tcggcgattt gcgcttgcgt
      421 ttcactggtg agcgcatcgc cgtttggtgc ggcggcgact ttgaagcccg cgtcgagtcc
      481 accgcgattc ccgccggtca cccttttctg ctccgcaccg gcgaagaatt aattattgac
      541 cggccgcgaa tcggatgccg cgcctggctc gcaatctccg gcgggatgga tgtgccgatc
      601 gttctcggca gtcgctcaac cgatttgcga ggaaactttg gtggtaccga tggacgagcg
      661 ctccgcgatg gcgacgaaat cccgcttgcc cgcaattctg aacgtgcaga cattttgatt
      721 gagcgacttc gtaaccaaaa ggtcgcgaac tggaaaccgc cccacgcttg gtccagcccg
      781 gtgcaacgcg aaccgctcct gcgcttcatc cgcggcgccg acttgcaccg cttcaatgct
      841 ttaacgcttc aacgcttcaa cgaggaaccc tttatcgttt caccggactc ggaccggatg
      901 ggtgttcgtt tcgatgctcc tgaattacag cgggaggatc atgtcgatct ggtctcggaa
      961 gcggtggcgc cgggcacggt gcaggtgccg ccgagcggca aaccgatcct gctgcttgga
     1021 gattgccaaa cgatcggcgg ctatccaaaa atcggacacg tgatcacggt cgatctgcca
     1081 atcgcggcgc aactgcgcgc cggtgatcag gtgcgattcc gcgaggtatc gctcgctgaa
     1141 gcccatcagc tctggctcga tcgcgaacgc gacctcgccc aattccgaca aggactggaa
     1201 gcacatttca catgaaaatg attgtcgatc ttaatgccga tcttggcgaa ggcgcagggc
     1261 acgacgacga gctgctcgcc attgtcagct cggcgagtat cgcgtgcggt tttcacgccg
     1321 gagatgccgg gacgatgcgc cgcgccattg aaacagcacg cgattatagc gtggcagtcg
     1381 gcgcgcatcc gagcttgttc gatcgccaaa atttcggccg caaagaactg gccgtcgcac
     1441 cggacgaagt tttcgacgcg gtcgcctacc agctcgggat cttccaagcc atcgcggagg
     1501 cggccggcgt tcgtccgaat cacgtcaaac cacacggcgc gctttacaac atggctgtgc
     1561 gcgatgaagc actcgccgac gcgatcggcc gcgccacagc gaagatggac aatgcgttga
     1621 tcctgttcgc gccggacggt agcgcgctcg cacggatggg tgaagcgaat ggcttgcaaa
     1681 tcgcgcgcga acttttcgcc gatcgtaatt atctcgacac cggctggctc gtgccccggt
     1741 cgcgaccgga cgcgttgttg cacgatccga tcgaagctgc cgaacgggtc attcgaatgt
     1801 tgcgcgaagg caaagtcaaa tcggtcgatg gagtagatgt cgatgttcgc ggcgaaacca
     1861 tttgtctgca cggcgacacg cccggagcag tcgaattcgc gcgcggtttg agatcgcggc
     1921 tcgagcagga aggcgtcacg atccgcgcac cgaaatcgaa tcgtctgtag ggcacgctga
     1981 cagcatcccg cgaacccagc ggcttcccta caggcgcgag atgaacgaca agatcgacaa
     2041 tcaacagccc agcggccttg ttcgagcgct tggcccgatc gacgccacga tgatcgtcat
     2101 cggatcgatg atcggctctg ggattttcat tacctcggcc gagtcggcgc ggttgaacgg
     2161 cgcaccgggc tggttgctgc tggcgtgggc cgtcgccggg ttgctcacca tcactggcgc
     2221 gctttgttgc tcggagctgg cgacgatgat gccgcgtgct ggcggggtct atgttttctt
     2281 ccgcgaagcg tacgggccga cactaggttt tctctacggc tggacgctgt tcctcgtcat
     2341 tcaaaccggc accatcgcgg ccgtagcaat cgcgtttgcc agatttctcg gcgtgtttgt
     2401 agacgcgatt tcgcctaaca attatctgat tgcgccggtg cagttaggca acgcctacgc
     2461 aatcagcctc tcaacccagc aattgatcgc gattgctttg atcgcgcttc tcacctggac
     2521 gaatacgcgc ggccttaaac tc
//
LOCUS       DASTDG010000099        11769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_522302, whole genome shotgun sequence.
ACCESSION   DASTDG010000099 DASTDG010000000
VERSION     DASTDG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522302"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..4753)
                     /locus_tag="VFO30_03120"
     CDS             complement(398..4753)
                     /locus_tag="VFO30_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418306.1"
                     /translation="MAIEQTILFTIIPRGIHANGATMPVSVFVSPRLNGADNLGAFGD
                     WVDWTRKLRDNGLSLGIQCGGNPPRDFPIDQAPLRPELWEQLFNEHTYVRSHDTFDDY
                     TDRAVISFSVRRTFSVLKTIYQQAGVALALPDQPGRDRSSNRRLLGELVNGLQVHWDG
                     RLANRLRETVRLTVQSPGFPGTQGIDAQLDSEGAIIGSIDPRASVTPFAVFHHMPALP
                     DKTYEPPKLPDPNKLLDFHQALSSLNSYPDLLRALGIIFDLDLPGDFVLDTTNTPFGT
                     LSVRGATPGWHWTTQPHTQPLATAYVKIRSGQKTLFFTAPRAVSGQLAPPLVLGLLDL
                     DPQHFGLAQVDVDGAMHKAIMLAESLNPSGAQNAAPGAQPEAAPHPEVFDPEATLPSL
                     RSGGFSIFADNRGLQLLASIQGTKAFNNALKSGPQPRPFFAEDLVRGYRLDVWDSRTA
                     QWHSLHRRNAKYQVGDVPFTPAAEPEEGFVQLAAAQPPPGAQPETNDLYLHEAIARWA
                     GWSLSAPRPGKHLSRYANAADAIPRDNEPDKFAEDQPETPFKVTAQYQVAKKSLPELR
                     FGRRYRFRARVVDLAGNSLALDDPLADLLADIFALPRDTEGFAYLRFEPVPAPLAVIR
                     DPKAVTDPGSAVERLVIRTFNNSIEKDHEPADTTAADRHIIPPRASIEVGEHLGVFDD
                     AGGRLIGDAATWKLIADRDAGELHTQSIAVASGPVKAYPVEPGGSIDPLPYFPDPLSR
                     GGAIRDLPGTPSGAIGRAVPDTGAAAAVAYDFLSDPNPRPGSATLISFGESGDWQKTR
                     GFRLTLAEPAKNQTDLRPHWDPIERVLTVYLPKGQTAVVPLTSYASVDDLKIMGVWQW
                     LRQLVEQITITQAHPQFLELDADEIAHVLQRAVEGGHWMLTPPRLLTLVHAVQQPLGR
                     PAFTALNIVRQNDAADSNALQTAPDRGRRDPTELASITAWRRLGATEAYLLGALKIHG
                     ASTARVDLLAAWDDPLDDLSQQKPSVVHHTAPVDELPMPSIAEGTLIAPGKDRRAVGY
                     YDPEHDQIAFTRSGDKSALDGNPSTSNALTFADAAPRHLLNDTKRHIVSYQAISTSRF
                     REYFDPKLDFTRTSKPIAVDVPASARPLAPDVVYVVPTFGWERQTDTNLKRSVRFGGG
                     LRVYLRRPWFSSGEGELLGVTLWSYANGSLNPVTRDKFKPFFTQWGMDPIWQTDNLSG
                     APDESNFAGVAEKDRDLSLEERSAADSAGNPGRVSVVGFEPQFDETRGLWFADVALNV
                     WTETYMPFVRLALVRYQPHALADAKISRVVLADFAQLTPDRSVMVTADPHHSRTIRVV
                     VSGIAPRGPQASVHTEPPPRNISATPTQIRVRVQQRDSAVASDLGWNDVPDATAKVAT
                     GFAGPDPSDDNLALWIGTVTFAERPAPDQFRLVIEEHEFISARFVVAENGAPAQPSRL
                     IFAEIFMLDNAIVGE"
     gene            complement(4761..8021)
                     /locus_tag="VFO30_03125"
     CDS             complement(4761..8021)
                     /locus_tag="VFO30_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418307.1"
                     /translation="MSSTTTVRTRLLRPDDLLHLQVEGLNMRLDSRQGSPVLVVKNPD
                     EPAYLRVVFSSQTIAETAVFEVSDFSGPGDVPVEPLFGPGLPGKDAKTVAQMAHPSRL
                     VFKVPPQTEIPFSTEGLLDWTKLELSVNGIAAIGDNPTSDQIAKAPAIAEPTETETAI
                     ELPYRLIISPTAEVAWLHRTSPFTSRGQTELWHTRLALKLKTQNNVDEQLLELSADQP
                     APLRAIWSPDFDSQELPLPRPDPDLGRTAMGNNDRHQIVVLTSAFHGYVADYTIEFPF
                     VSPFALGPRISTIRDFGTISLVRTGPFVPQPFHTTELMLSTLGGWLRSRGHWDPLPRP
                     APPPIFKPPDFGEIFKGLANLGESRPVERIAPDIAIGPVIIPGLPQPSQSLDLSEWVH
                     IAAQGRDHYVRIVHEGELWPFRNRAALIQVTERKFEEKNGIVVAYLMQRMFIVVREPE
                     KTFATQDFGNPFKRVRLTTLVTPDIAQPELIGGTNNTFWVKVFVGAGSTDFFRFHAVG
                     NDVEANTVDFTVPMLFVSVSDLTSSKPLDEYNAPVNAPADGRFRACIVPGQKVAFAPA
                     SKTQANENTYLATETLNFVMDDARVQPTLLKATVRIPQVRDLLGADLPTTIRLFPDFV
                     RSAQGLDDPANKTGVFAQVVDDNAYTAIDPNNVTAALKKLQVDIHADQAGGFATPSLG
                     VATLSRALGPLAGDVSNAVADTFNPAQFFPKDGTQLFGAFDLLALLGATSASENAPKM
                     HTAPEGSDLVTRFEWNPKIGPVEISGIAAFKPDDEKGLTVNGTIRKPINPPAGSSGDV
                     TAEFTGTLKKFHVSVLDSVLINFVEFSFTKKSKEKPDVTVQLDPGTPVEFTGDLKFIE
                     EIRDAIPPGLFGDGPSLDLLPTGIRAGFAFALPPLTVGVFALKDVKLDAGLTLPFIEG
                     KPVFDFNISERPHPFLLTVAIFGGGGFFHLQLDTAGMKELEVALEFGAAAALDIGVAS
                     GEVHVMAGIYFSLQRKENTTQLAAVLSGYLRMGGSLNVLGLVKISVEFNLSFTYDSGR
                     DKAYGRATLTVQVEVLFFSTSVELTVERAFGGSGDPHFIDFFPKPEPWEEYALAFA"
     gene            complement(8404..9120)
                     /locus_tag="VFO30_03130"
     CDS             complement(8404..9120)
                     /locus_tag="VFO30_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9418308.1"
                     /translation="MDEYISDDPIKEVRWGQHLAESSPVPQAMVARVDFLDPHLEERL
                     DIYHSLPNVTAVREHLGWDVTNPKRRFATRPDLLTDPFWQRGLGALRRYNFKCGLEIF
                     SPQIPDLLKVVRLYPDLGFTIALLGWPLDLSTAGYETWLQDLKELSQCENVCAAISAI
                     ECIFGLDWRADFVGPWILSLIEMFGPKRCMFGSHLPISKLSFGFDRLYDAYQHIVGEF
                     SQDEKDHLFRKVAADWFAIP"
     gene            10233..11123
                     /locus_tag="VFO30_03135"
     CDS             10233..11123
                     /locus_tag="VFO30_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9418309.1"
                     /translation="MARALVSSRTHAIGYIVDNLSWSFFSELAEGIQKAAESFHYSSY
                     IHSSLKHPENERQGIESLLSRGVEGILISPTEAKENLEIIQDLQAQKFPLVLLSDIEE
                     LEVDKVVVDNYAGAYRAMEHLHSLGHRKIMYIGPAENGTIKTQRLRAYHSYIERHQLA
                     ESKGLLFCEENDPLYGYRVVRQVMKTKQRPTAIFAHNDTLALGVYRGLYEMGFKIPDD
                     ISVVGYDDLPSCEFVHPPLASVGTPLAELAAIAVSLLMERIEQFNSEGFKTGTAAVRQ
                     KIALNPKLVVRQSTGKPPAA"
     gene            11120..>11769
                     /locus_tag="VFO30_03140"
     CDS             11120..>11769
                     /locus_tag="VFO30_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9418310.1"
                     /translation="MTNNKNATAGMYRIVNNPIKLGIIGLHNHYHIYPMADYLAKGIP
                     GLEMVSVYDERTELADRFAKQYSVPEVHDSRESLLKRSDIDAVLIMSDTASHRQDLIA
                     CAKHKKHVLLDKPIALNVKEATEMIQAAEEAGIIFQMAYLIRYLPSYRKARQLIRDGV
                     IGKPLTMKIGIRCPLKFIRDNPDATEPGWYSDPKRGGGGGFMDHGIHYSDTLRFLLG"
BASE COUNT         2530 a         3381 c         3466 g         2392 t
ORIGIN      
        1 ctgaaacggc atctgcgacg atgcagtcaa cgaccctgga aagcacctgc tgacctcacg
       61 gtgtaccagt acttccaaaa gctcggactg atctatctgt gaatgctccg tgcaatacgc
      121 tcacgcgaaa gccgtatgcg gcaaatccgc acgtacggtt tgacgaggga gagggctcct
      181 cactggaccc tctctactct acttcaaaac ctctttgttc ttctcaacga gaaatggcct
      241 tcgaacagct tgaagattgt agcagaaacc gacttaacct tcggctgcaa gccgtacgaa
      301 acagcgagca ggcacgaatc gtgacaaccg agtcggctcc cgcctctgat acctgaagag
      361 gccggcatct gctcatgcag cttggtgcgc ggaccagcta ctcaccaacg attgcattgt
      421 cgagcatgaa gatctcggcg aaaatcaacc ggctcggctg tgccggtgcg ccgttttcag
      481 ccaccacgaa tcgcgcggag atgaattcgt gctcttcgat gacgagccgg aactgatctg
      541 gcgcaggcct ttccgcaaaa gtgaccgtgc ctatccacag cgccaaattg tcgtcagatg
      601 gatcgggacc ggcgaatccg gtggcgactt tcgcggtcgc atcgggtaca tcgttccacc
      661 caaggtcgct cgcgaccgcc gaatcccgct gttgcacgcg cacgcggatt tgggtcggcg
      721 tagcactgat gtttctcggc ggcggctcgg tatgcacgct tgcctgtggc ccgcgcggag
      781 caatgccgct aacgacgacg cggatggtgc gcgaatgatg cggatcggcg gtgaccatca
      841 cagagcggtc gggcgtgagt tgggcgaaat cagcgagcac gacccggctg attttggcat
      901 cagccagtgc gtgcggttgg tagcggacga gagcgagccg cacaaatggc atgtaggttt
      961 ctgtccatac gttcaacgcg acatcggcga accataagcc gcgcgtttcg tcgaattggg
     1021 gctcgaaacc cacaacggaa acgcgtccgg gattgcccgc ggaatcggcg gccgaccttt
     1081 cctcgagcga taaatcgcga tccttttcgg caactccggc gaaattgctt tcgtcaggtg
     1141 cgccgctcag gttgtcggtt tgccagatgg ggtccattcc ccattgggtg aaaaacggtt
     1201 tgaacttgtc gcgtgtgacc ggattgagcg agccgttcgc atagctccaa agcgtaacgc
     1261 cgagcagttc accctcgccg gacgaaaacc acggccggcg caaatagaca cgcaatccgc
     1321 cgccgaagcg tacactgcgt ttcaggttcg tgtcggtctg ccgctcccag ccgaaagtcg
     1381 ggacgacgta aacgacgtcc ggtgccagtg gtctcgcgga agccggaaca tcgacggcga
     1441 tcggtttgct cgtacgcgtg aagtcgagct ttggatcgaa atactcgcgg aagcgcgacg
     1501 tggaaatcgc ctggtagctg acgatatgcc gtttggtgtc gtttaaaaga tggcgcggcg
     1561 cagcatcggc gaaagtaagc gcattgctcg tcgatgggtt gccgtcgagc gcggacttgt
     1621 cgccgctgcg ggtgaaagca atctggtcgt gctccggatc gtaatagccg actgcgcgcc
     1681 tatcctttcc gggtgcaatc agagttccct ctgcgatcga gggcatcggc agctcgtcca
     1741 ctggcgccgt gtgatgaaca acggacggtt tttgctgcga tagatcatcg agcggatcgt
     1801 cccaggccgc gagcagatcg acgcgggcag tgctcgcgcc gtggattttg agcgctccaa
     1861 ggagatatgc ctcggtcgcg ccaaggcgcc gccacgccgt gatcgacgcg agctcggtcg
     1921 ggtcgcgcct tccacggtcg ggcgcggtct gaagcgcatt cgaatcggcg gcgtcattct
     1981 gccgcacaat gtttagtgcc gtgaaggcag ggcggccgag tggctgctgc acggcatgca
     2041 ccagtgtcag caagcgcggc ggggtcagca tccagtggcc gccctcgacg gcgcgctgga
     2101 gcacgtgcgc tatctcgtcg gcatcgagct cgaggaattg cgggtgtgcc tgcgtgatgg
     2161 tgatttgctc gacgagctgg cgcagccatt gccaaacgcc cattattttc aggtcgtcaa
     2221 cgctcgcgta actcgtcagc ggaacgaccg cggtctgacc tttgggcaaa tacacggtca
     2281 atacgcgctc gatcgggtcc caatgcggcc gcaggtccgt ctgatttttt gccggctcgg
     2341 cgagcgtcag acgaaacccg cgcgttttct gccagtcgcc gctttcgccg aagctgatga
     2401 gagtcgcgga gccaggacgc ggatttgggt cgctcagaaa atcataggcg accgccgcgg
     2461 cagcaccggt atccgggacg gctcgtccga tcgcgccgga gggcgtgccg ggcaggtcgc
     2521 gaatcgcccc accacgtgag agcggatcag gaaaataggg gagcggatcg atgctgccgc
     2581 ctggctcgac cggataagct ttcaccgggc cgctggcgac agcgatcgac tgggtgtgga
     2641 gctcacccgc gtcgcggtcg gcgatcaatt tccatgtcgc cgcgtcgccg atgagcctgc
     2701 cgccggcgtc gtcgaatacg ccgaggtgct caccgacttc aatgctcgcc cgaggcggaa
     2761 tgatgtgccg atccgcagcc gtcgtgtctg cgggttcgtg atccttttct atcgagttat
     2821 tgaacgtccg aatcaccaga cgttcgaccg ccgagccggg atcggtgact gccttcggat
     2881 cgcggatgac ggctaacggg gccggcaccg gctcgaagcg cagataagcg aatccctccg
     2941 tgtcgcgcgg cagcgcgaaa atatccgcga gcagatcggc gagcggatca tccaaggcaa
     3001 ggctgtttcc ggcgaggtca accacacgtg cgcgaaaccg ataccggcgc ccgaagcgta
     3061 attccggtag cgattttttt gcaacctgat attgcgccgt gaccttgaaa ggcgtctccg
     3121 gctggtcctc ggcgaatttg tccggctcgt tgtcgcgtgg gatcgcgtcg gccgcgttgg
     3181 cgtagcggct gagatgtttg cccggacgcg gtgcactcag gctccagccg gcccatcgcg
     3241 cgatcgcttc gtgcaggtag agatcgttcg tctccggctg cgcaccgggc ggtggttggg
     3301 cggcggcaag ctgcacgaag ccttcttctg gttccgccgc tggcgtgaaa ggaacatctc
     3361 cgacttgata ttttgcgttg cggcggtgca gcgaatgcca ttgcgcggtg cgcgagtccc
     3421 agacgtcgag tcgatagccg cgcaccaggt cttccgcgaa aaacgggcgc ggctggggac
     3481 cgctcttcaa cgcattgttg aatgccttcg tgccctgaat gctcgcgagt agttgcagcc
     3541 cgcgattgtc ggcgaagatc gaaaaaccgc ctgaccgcag cgaagggagt gtcgcctcgg
     3601 gatcgaaaac ctcagggtgc ggcgcggctt ccggctgcgc gcccggcgcg gcattctgcg
     3661 ctcccgaggg attcagcgat tcggcgagca taatcgcctt gtgcatcgcg ccatcgacat
     3721 cgacttgcgc caatccgaag tgctgcggat cgagatcgag caacccgagt accaacggcg
     3781 gcgccagttg gccgctcaca gcgcgtggcg ctgtaaaaaa gagcgtcttc tgtccggatc
     3841 gaattttgac gtaagccgtc gccagcggct gggtatgcgg ctgcgtagtc cagtgccagc
     3901 cgggagtcgc gccacggacg gagagtgttc caaacggtgt gttagtcgtg tccagcacaa
     3961 aatcacccgg tagatcgagg tcgaaaatga tgccgagcgc gcgcagcaag tcggggtaag
     4021 agttcagcga actgagcgcc tggtgaaagt cgagcagctt gttcggatcg ggcagcttcg
     4081 gcggctcata tgttttgtcc ggcaacgcgg gcatatgatg aaagacggcg aacggcgtca
     4141 cggaggcgcg cgggtcgatg ctgccgatga tcgcgccctc gctgtccagt tgcgcgtcga
     4201 taccctgcgt ccctggaaaa ccgggcgact gcaccgtcag acgcacggtt tcgcgtagcc
     4261 ggttcgcaag tcttccgtcc caatgcacct gcaagccgtt caccagctcg cccaatagcc
     4321 ggcgattgga acttcgatca cgcccgggtt ggtccggcag agccagtgct actcctgcct
     4381 gctggtagat agtcttgagt acactgaaag tccgtcgcac cgaaaacgaa ataaccgcgc
     4441 gatccgtgta gtcgtcgaac gtatcatgcg agcgcacgta ggtgtgctcg ttgaaaagct
     4501 gctcccacaa ctcgggccgc aacggcgctt gatcgatcgg gaaatcacgc ggcggatttc
     4561 caccgcattg aatgccgagg ctcagaccgt tgtcccgcag cttccgcgtc cagtctaccc
     4621 agtcaccgaa agcgccgaga ttgtccgcgc cgttgagccg cggggaaacg aacacggaga
     4681 ctggcatcgt tgcaccattc gcgtggatac cgcgcggtat gatggtaaaa aggatcgttt
     4741 gctcgatagc catgtttttt tcaggcgaac gccagcgcgt attcctccca cggttccggt
     4801 tttggaaaaa aatcgatgaa atgcgggtcg ccgctcccgc cgaatgcgcg ctcgacggtc
     4861 agctcaactg aagtgctgaa gaagagcact tcgacctgta cggtgagtgt tgcgcgccca
     4921 taagctttgt cgcgcccgct gtcgtaggta aaactcaggt taaactcgac agagattttg
     4981 accagtccga gcacgttgag cgagccgccc atgcgcaaat aaccggatag caccgcagcg
     5041 agctgggtcg tattttcctt gcgctggagc gaaaaataga tgccggccat tacgtgcacc
     5101 tcgccactcg cgacgccgat gtcgagcgcc gcggccgccc cgaattcgag cgcgacttcg
     5161 agttccttca tgcccgcggt gtcgagttgg aggtggaaaa aaccgccgcc gccgaagatc
     5221 gcgaccgtga gtaaaaaagg atgcgggcgc tcggaaatat taaaatcaaa aacgggcttg
     5281 ccctcgatga acggcagcgt gagcccggcg tccagcttta cgtccttcaa cgcaaagacg
     5341 ccgacggtca gcggtggcag ggcgaaggcg aaacccgcac ggatgccggt tgggagaagg
     5401 tcaagacttg gcccgtcacc aaacaagccg ggtggaatcg cgtcgcggat ttcctcgatg
     5461 aatttgagat cgcctgtgaa ttcaaccggg gtccccgggt cgagttgcac cgtcacgtcc
     5521 ggcttttcct tcgacttttt tgtgaagctg aactcgacga agttaatcag caccgaatcg
     5581 agtacgctca catggaattt cttgagcgtg ccggtgaact ctgcagtcac atcgcccgac
     5641 gagccggcgg gcgggttgat cggcttgcgg attgtgccat tgacggtcag gcctttttca
     5701 tcgtcgggct tgaacgccgc gatccccgag atctccacgg ggccgatctt cgggttccac
     5761 tcgaagcggg ttaccaggtc tgagccttcg ggcgccgtgt gcatcttcgg cgcgttttcg
     5821 ctggcgctgg tggcccccag cagggcgagc agatcgaacg cgccaaaaag ctgggttcca
     5881 tctttaggga aaaactgcgc cggattgaaa gtgtccgcaa cggcgttgct cacgtcgccg
     5941 gccagaggtc cgagcgctct agacagggtg gcgacgccaa gactcggcgt cgcaaatccg
     6001 ccggcctggt cggcgtgaat atcgacttga agtttcttaa gcgcggcggt gacgttgttc
     6061 gggtcgatcg cggtgtaggc gttatcgtcc acgacctgcg cgaacacccc cgtcttgttc
     6121 gcaggatcat caaggccttg cgcgctacgc acgaagtccg gaaaaagccg gatcgttgtc
     6181 ggtaaatccg caccgagcag atcacgcact tgcgggatgc gcaccgtcgc cttaagcagc
     6241 gttggttgga cgcgcgcgtc gtccatcaca aagttcagcg tttcggttgc gagatacgta
     6301 ttctcgttcg cctgtgtttt gctggccggc gcaaacgcga ccttctgccc cggcacgatg
     6361 cacgcacgga acctgccgtc cgccggtgcg ttcaccggcg cgttgtattc gtccagtggt
     6421 ttggacgagg tcaagtccga aaccgagaca aacagcatcg gcaccgtgaa atcgaccgtg
     6481 ttggcctcga cgtcgttgcc aacggcgtga aagcggaaaa aatccgtcga tccggccccg
     6541 acgaaaacct tcacccagaa cgtattgttg gttccaccga tcaattccgg ctgagcgata
     6601 tccggcgtca ccagggttgt aagccgcacg cgcttgaacg gattgccgaa gtcctgcgtg
     6661 gcaaacgttt tctcgggctc gcgcacaacg atgaacatgc gctgcatcaa ataggcgacg
     6721 acgatcccgt ttttctcctc gaactttcgc tcggtcactt ggatcagcgc cgcccgattg
     6781 cggaagggcc agagctctcc ttcgtgaacg atccgcacat aatgatcgcg cccctgtgct
     6841 gcgatgtgca cccattccga cagatccagc gactggctcg gctgcggcaa cccgggaatg
     6901 atcaccggac cgatcgcgat gtccggcgcg attcgttcga ccggacgcga ttctccgaga
     6961 ttcgccagcc ctttgaaaat ttcgccgaaa tccggcggct tgaaaatcgg cggtggcgcc
     7021 ggtctcggca gtggatccca atgcccgcgc gatcgcagcc agccgccgag ggtgctaagc
     7081 atcaattcgg ttgtgtgaaa aggctgcggc acaaacggcc ccgttcgcac cagcgagatc
     7141 gttccgaaat cgcggatcgt gctgattctg ggacccagcg caaacggcga cacgaacggg
     7201 aactcgatcg tgtagtcggc cacataccca tgaaacgccg aggtgagcac aacgatctgg
     7261 tgccggtcgt tgttgcccat tgccgtgcgg ccgaggtcgg gatcgggccg tggcaggggt
     7321 aattcttgcg aatcgaaatc gggcgaccat atcgcacgca acggcgcggg ctgatcggcg
     7381 ctcaattcga gtagttgttc atctacattg ttttgcgtct taagcttcaa tgccagccgc
     7441 gtgtgccaca gctcggtctg gccacgggaa gtaaatggtg acgtccgatg cagccaagct
     7501 acttccgccg tcggcgaaat gatcagccgg taaggcagct cgattgccgt ctcggtctcg
     7561 gttggctcgg cgatcgcggg tgccttggcg atttggtccg atgttggatt gtcgccgatg
     7621 gccgcaatcc cattgacgct tagctcgagc ttggtccagt cgagcaatcc ctcggtcgaa
     7681 aacggaatct ccgtttgggg cggcactttg aaaaccagcc ggctcggatg agccatttgc
     7741 gcgacggtct tcgcatcctt gcccggtaat ccgggcccga acagcggctc gaccggcacg
     7801 tcacccggcc cgctgaaatc gctcacttcg aaaaccgccg tctccgcgat tgtctgcgag
     7861 gaaaaaacca cgcgaaggta agcgggttca tccgggttct tgactacgag aaccggggag
     7921 ccctgccgtg aatcgagccg catattcagc ccttcgactt gcaaatggag caagtcatcg
     7981 gggcgcagga gcctggtgcg aacagtggtt gtcgaggaca tgttcggctt caacgggtga
     8041 tcacacgtat cgccatcgtc ctcaatgtta aggctcagag agaatctgta tatacgtcag
     8101 atgacgtacg cgccgcgttg gcccgaaccc acgaggggtc aatcagccaa caaacaaaag
     8161 tttggcgcga tcgtgttcgc aaactctctt ggtcaaggtg cctacctgca tcgcgattcg
     8221 taaggtggct ctccgcccca gcctacctcc gtcggaggca cgagcctatg taaacgcgag
     8281 gacgagacga gtatccgata tagtagccag gacgagcaac ctcggacaca attaccggac
     8341 gtcgagtcgc ccagcactgc tcgctcgtac aggagcgcgc tgaacaagcc gcggctcctc
     8401 tatttatgga atggcaaacc agtcggccgc caccttgcga aacaagtggt ctttctcatc
     8461 ctgagaaaac tcgccgacaa tgtgctggta ggcgtcataa agccgatcga agccgaacga
     8521 cagcttggag atgggcaggt gactcccgaa catgcaacgc tttgggccaa acatctcaat
     8581 gagcgacaga atccatgggc caacgaaatc agcgcgccaa tctaatccga aaatgcactc
     8641 aattgccgag atagcggcgc acacgttttc gcattggcta agttctttga gatcctgaag
     8701 ccatgtttcg taacccgctg tgctcaaatc tagaggccat cccagaagcg cgatggtaaa
     8761 gccgagatct ggatagagcc gaactacctt aagaaggtcg gggatctgcg gcgaaaagat
     8821 ttccaggccg cacttgaaat tgtagcggcg tagggcgcca agcccgcgct gccaaaacgg
     8881 gtcagtgagc agatcggggc gcgtagcgaa tcgccttttt ggattcgtca catcccagcc
     8941 taggtgttca cgtacggccg tcacattcgg cagcgaatgg tagatgtcga gtcgctcttc
     9001 aagatgtgga tcgagaaagt cgacgcgggc caccatggcc tgagggactg gcgaactctc
     9061 cgccaggtgc tgcccccatc ggacttcctt gatcggatcg tcagaaatgt attcgtccaa
     9121 acgatgccgt ccacttggca cgagctgcta tcctttagat agccgtcaag caaataactc
     9181 ctgggaagag aggagtagtc gcccaccagc gcctcgtaga cttcatcttt ctgattgagg
     9241 aagccatgat tgttggcgtt cccatcgtat agatgcacgt gggcgtcgac aatgggcagc
     9301 ctatagcctg aaatcgatga ttcgttactc atcaccgtaa ggtctatcga ttaggcgata
     9361 caacggcaag gaagcgcctc gaacttcgtg ttcccatgct tccaggcgat tccgagcgga
     9421 cgatcgaagg ccaaggttac gaaaatgcac cctcagagtt tcgaccgcga accggcgggc
     9481 gtgatccagt gtcggggtcg atggagggtc gtaggggtcg agtctaaaaa gaatcaagaa
     9541 tctggaaccg atggctgcag aactgcgctc tgggtgacag gaaccaagaa atatgatttt
     9601 gcgatatcag agtccttgaa tcttttagac gcgacctcat taacccgacc cttcatgctc
     9661 aggcacgtcc gtacccgctc ctcgtccaaa aatggaatcg tagctttctt cgacctcgca
     9721 ttctcatttt cattcagtgt gggccgagaa tgaggttggt tcgagccgat cccagaggag
     9781 aagcgcaaat ggtgtacgat ccgacttcgc ttccatcaca caagctgacg cgctggcctg
     9841 gagctaggta ggacaaacta ttcgctgcca gcactacacg ggattttcgg cgtttatcgc
     9901 aaaaagaaaa ttgacaaaaa taaataaatc gccagactgt gcgaacgtta gcacaaaatc
     9961 acgcgataac ccctaattta atcttgtttc cttgattgat aaagcttgtg cgaacgttag
    10021 cacaattgaa tccccgtttt atcgttttta gtgccgaacc tccaaccgtc cctctaaccc
    10081 ccgtgaaggt taccatcacc gatatcgcca ctgtcgtgaa cgtttcgccg actaccgtat
    10141 cgcgtgcgct caaaaacgat tcgcggatca caccggaggt gcgcacaaaa gtcaaaaacg
    10201 ctgctcaaaa gctgggttat cggccgaacc tgatggcccg cgcgctggtc agcagccgta
    10261 cccatgcgat cgggtacatc gtcgataacc tttcctggtc attctttagt gaactggctg
    10321 aaggtattca aaaggccgcc gagtcgttcc actactcctc ctatatccat agcagtctca
    10381 aacaccctga gaacgaacgt caaggcattg aaagcctcct gtcccgtgga gtcgaaggta
    10441 ttttgatttc gccgaccgaa gcgaaagaga acctggaaat tatccaggat ctgcaggcgc
    10501 aaaaattccc attggttctc ttgagcgaca ttgaagagct cgaggtagac aaagttgtcg
    10561 tcgataacta cgccggcgct tatcgggcaa tggaacactt gcacagtcta gggcaccgaa
    10621 aaatcatgta tatcggaccc gcggaaaacg ggaccatcaa gactcagcga cttcgcgctt
    10681 atcattcgta catcgaacgg caccagctgg ccgagagcaa agggctgctg ttctgcgaag
    10741 aaaacgatcc gctgtacggt tatcgcgtcg tacggcaggt catgaaaaca aagcagcgtc
    10801 cgactgcgat attcgcgcac aacgatacgc ttgcgctcgg tgtttatcgt ggcctctatg
    10861 aaatggggtt caagattccg gacgatatct cggtcgttgg ttacgacgat cttccttcct
    10921 gcgaattcgt gcatccgcca ctggccagcg tcgggactcc gcttgccgag ctcgcggcga
    10981 tcgcggtcag cctcctgatg gaaagaattg agcaattcaa ttcggaagga ttcaaaactg
    11041 gaaccgcggc ggtacgccag aaaatagctc tgaacccaaa gttagtcgtt cggcaatcca
    11101 ccggcaaacc accggcggcg tgactaacaa caaaaacgca acggccggca tgtaccgtat
    11161 cgtgaataac ccaattaaac taggtatcat cgggcttcat aaccactatc acatctaccc
    11221 aatggccgac tatctggcca aagggattcc cggactagag atggtctccg tttatgacga
    11281 aaggacggaa ctggcagacc ggtttgctaa acagtactcg gttccagaag tccatgactc
    11341 tcgggaatcg ctcctgaagc gatccgatat cgatgccgtg ctcatcatga gtgacacggc
    11401 cagtcaccgg caagacctga tcgcttgcgc gaaacataag aagcacgtgc tgctggacaa
    11461 gccaatcgcg ctgaatgtga aagaggcgac cgaaatgatt caggctgcag aagaagccgg
    11521 gattatcttc cagatggctt acttgattcg gtatctgccg tcttaccgga aagccagaca
    11581 actgatccgc gacggtgtca tcggcaaacc gctcaccatg aagatcggaa tccggtgccc
    11641 gctgaagttc attcgcgaca atccagatgc taccgaaccg ggttggtact cggatcccaa
    11701 acgtgggggt ggaggcggat tcatggatca cggcattcac tattccgata cgctgagatt
    11761 cctgttggg
//
LOCUS       DASTDG010000100        14898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_455348, whole genome shotgun sequence.
ACCESSION   DASTDG010000100 DASTDG010000000
VERSION     DASTDG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455348"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFO30_03145"
     CDS             <1..483
                     /locus_tag="VFO30_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9418311.1"
                     /translation="ESAAGLAAAPLTKARLQIKEIRGVQFIDDSYNANPDSMKAALRT
                     LVELDTAGKRIAVLGEMRELGEESERAHREIGETAADLRVNQLIAIGEAAATIVNGAR
                     EAGLRNVATAKSTTEAAQLLADAVAPGDLVLVKGSRAARTELVIEEFAKRQPTEGVPP
                     "
     gene            480..1580
                     /gene="mraY"
                     /locus_tag="VFO30_03150"
     CDS             480..1580
                     /gene="mraY"
                     /locus_tag="VFO30_03150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9418312.1"
                     /translation="MMYYLHRFSSQFGGFNVFLYVTFRAVAAAVTAFLISILFGNFVI
                     RKLTALKLGQPIRLAREVHRLAELHGGKQGTPTMGGVLIIGSVFISSVLWARLGNRFV
                     ALALFAMLYLGALGFVDDYLKVTKKKSAGVSGRWKLLFQIVLAVVITTVFLSSPLLEV
                     QARSLYVPFVKAPVVDNMGWFTFLFFTLVIVGASNAVNLTDGLDGLAIGCTVTVALAY
                     AVLSYVAGNFRVAEYLQVPFYPFTAELAVVCAALTGAGFGFLWFNCHPAKVFMGDTGS
                     LAIGGLIGVVAICCKQELLLVVVGGVFVIEAVSVILQVISFKTTGRRIFLMSPLHHHF
                     ELAGWKENTVIVRFWILSGICALLGLATLKLR"
     gene            1585..2916
                     /gene="murD"
                     /locus_tag="VFO30_03155"
     CDS             1585..2916
                     /gene="murD"
                     /locus_tag="VFO30_03155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9418313.1"
                     /translation="MRYKNQHVAVLGAGLSGTAAALLLRSEGAEVAVLDNAEEKNLLK
                     STLENLRAQGVRVICGPAANADQTKYDFAVLSPGIDPLSPLALNFSSRKIDITGELEL
                     GWRSCGLPVIAVTGTNGKTTTTELLAQMLNACGQRTIACGNIGKPLSEVAREKEPFDV
                     LTVEVSSFQLETIKTFRPSIALWLNFAPDHLDRYRSVADYRAAKLRIFENQTPDDVAI
                     VNVLDNLRDLRARIISFSAYSDRADFRLAGGAIVYENKPVLRLSQTKLRGSHNIENLM
                     ATLAAGMARGLSFEQMAEPLSAYEPRPHRCEFVRQIGGIDYINDSKATNLDAVEKALL
                     AQSKPVVLIAGGKDKGFDFQPLRRVVKEKVRSTVLIGEMAERIARDWSGAVTSEIAGS
                     LADALERARAVAKPGDVILFSPGTSSFDMFKSYADRGDQFRALVQALPQQP"
     gene            2913..3428
                     /locus_tag="VFO30_03160"
     CDS             2913..3428
                     /locus_tag="VFO30_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9418314.1"
                     /translation="MKLSRILKKLRATTIRRPLRTSTQANYDDMSEPNMKLSRALLVV
                     LLLHVLAVSGIIAFNAIKTRQTAAAASTASPSATPATKPSQVAAASTSATAGKTEHAK
                     KGESVKLAADSHKVYVVAKGDNPVTIAKKFKVSYDDLIALNHIDDPRKLRVGQKLLIP
                     TRTTKAKKTAD"
     gene            3442..4581
                     /gene="ftsW"
                     /locus_tag="VFO30_03165"
     CDS             3442..4581
                     /gene="ftsW"
                     /locus_tag="VFO30_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9418315.1"
                     /translation="MHRKSAYILFVAVLAMLVIGIVMLFSTSAFARDSHGNMYFFIRR
                     QAIWLGIGLTLCAAAALIDYHFWQRTWWLWFSLAAIALALCFVPHVGMRINGSRRWIA
                     IAQLGLQPSEIAKIAAVFFLAAWFSRHENASGTIVYGFVLPLTITGLLLALILFEVDL
                     GTTILLGAATLVVMFVAGVNPILLGSVSLAGLGAILLLATHISERMSRLSAFLDPQRF
                     KQDAGLQQMQALIAWGSGGVEGLGLGNGRQKMLYLPYAHTDFIFPIIGEELGLRVSLA
                     VIFLFVLIIICGMMIALHAKDRFGLLLGCGIVSLLILQAAVNIGVTTSLLPNKGLPLP
                     FISYGGSNLVACLFGVGLLLNIYRQGVLEPKQKGTALQARTTPRI"
     gene            4723..5817
                     /gene="murG"
                     /locus_tag="VFO30_03170"
     CDS             4723..5817
                     /gene="murG"
                     /locus_tag="VFO30_03170"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9418316.1"
                     /translation="MNAVIACGGTGGHLFPGIAVAEVLDQRGHEVMLFVSEKEIDSLA
                     LSTRSQFRFEKLPVVGLPSLYSPAIFGFIRRFSESLSRCRAIYEKFQPQAVLGMGGFT
                     STAPILAGRLRGASTFIHESNAVPGKANRMTARWARAVLLGFKECAPFFPKARTEVTG
                     TPIRTELRRLDRRVARQKLGLRDDLPTLLVMGGSQGASGINQALIKSLASLREMALQV
                     IHLSGARDERLVADNYRRENIPAFVAAFHQGMEEVYSAADLAVARAGAASLAELACFA
                     LPGILIPFPYATDDHQTRNAEIFARAGAAILLKESEIQGDSLARKIKELIADATKLRR
                     MSEICSRLAPKDAAERVVATIEKYTNDGRN"
     gene            5801..8098
                     /gene="murC"
                     /locus_tag="VFO30_03175"
     CDS             5801..8098
                     /gene="murC"
                     /locus_tag="VFO30_03175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9418317.1"
                     /translation="MTAAIDLDLATCLTRERHRVHLVGVAGSGMSGLAGLLLQLGHEV
                     RGSDKVTTLETERLQRLGLQFHEKQHADEAHDAQLMIFSSAIKFDNAILANARAAGIP
                     IVRRAEALAAIMSCKRGILIVGMHGKTTTSAMAAHVLREGGLHPSHYVGAEIPILGTN
                     AHWDARGEYFVAEGDESDGTLKYFRPEHTLVLNIEAEHLDFYPDLGAIEKAFVELIGQ
                     TSGKIFYSADDPIATRICRSCTGAIGFGLSLDAEYRAANIRLQDFVSTFYIYRRSERL
                     GEAVLNVPGEHNVHNALGVIALASELDVPFAKIASALRKFQHARRRFEIKYASDRFLL
                     VDDYGHHPTEIRATLKTARAIRRKRVLTMFQPHRYSRTKALAKEFGCAFDDADRIVVT
                     DVYPASEAPMAGISGQTIVDEIVAHGHRGVTYQPRLDSVHRDIGNMLDSGDLVLSLGA
                     GDIHEQLSILAADLVVAEKVKEIVGDEGDVRLYEPLSRHTTLRVGGPAQFWVEPRTEI
                     AFAELIRFARRENLPLFVIGRGSNLLVRDGGIRGLVVHPSGGEFDKIDINGQEITAGV
                     GVKMKEVAYAARSAGLGGLEWMEGIPGAVGGGLRMNAGAMGAQLCENLVRLRYLDAEG
                     NRHVQDRHELDLRYRDFPLLHNNYAISATFRGVPAPREQIEARLQESQEKRRTTQPIA
                     KSAGCIFKNPDSSPAGKLVEELGLKNSRIGNARVSEVHGNFIVNDGGATAAEMLELID
                     RIKTAALTKGGIQLETEVQIVGEMA"
     gene            8098..9024
                     /locus_tag="VFO30_03180"
     CDS             8098..9024
                     /locus_tag="VFO30_03180"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9418318.1"
                     /translation="MEVPKKFAVLMGGPGSERDVSLATGRGVAKALRSLGVEVLELDV
                     RDGNFTLPSDVDLAFLTIHGTFGEDGQLQRILEERGVAYTGDGVEPTATAIDKIRSKR
                     KFDEHGVATPRWEVIQPNQRPSISLPIVVKPPREGSTVGVIIVKNERELESAVREAAN
                     YDRELLVEKFVPGRELTIGILGDQALPILEIIPKGGFYDFKNKYPFLNPQAGGGAEHV
                     CPAKIDKDKTRQIQELALRAFRALGLQVYGRIDVILSDRGEPFVLEVNTIPGMTEASL
                     LPEAAAAAGISYIDLCLRIIELSRARMEKKAR"
     gene            9099..10085
                     /locus_tag="VFO30_03185"
     CDS             9099..10085
                     /locus_tag="VFO30_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9418319.1"
                     /translation="MKVRSRTATQHRTRRVVVLVSKITLALGLCVGIYFGVREGARRL
                     FFENADYQLRAIEVQTDGTLARDQILQIAGLREGENIFSINLAKVHDDLQQLPQVDDV
                     QVVRKLPAEIDIKVTERKPVAWITEEKELSDPFNADSAFLVDGRGVLMKQKKLLPEYL
                     GLPLIVGCIGESFEAGNVVESPEAKAALELLRLSTRSFMQTRFQVREIDISKGYCLVV
                     TDKNHTRVTFAFDKLEEQLQRLEQFLVYSDDTKRELATVNLLVQRNIPVTFAKPAAEV
                     INDTIYPEATPRILKAIPVRPARAGESVPARSKSTSTPSVRRAIPVAQEKKN"
     gene            10088..11293
                     /gene="ftsA"
                     /locus_tag="VFO30_03190"
     CDS             10088..11293
                     /gene="ftsA"
                     /locus_tag="VFO30_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9418320.1"
                     /translation="MASGDLMVGLEIGTSKVCVVVGEGRPDGTLKILGVGQAPSRGIR
                     KGEIVDFETAMKCVHEAVVDAEQKSDVMIRSVYVAVAGAHIQSFNNRGCVVLPEDRDE
                     MDEQDIEDVKVNAREVSIPAQNAFLHSIIQHYHVDGQDGVLNPVGMLGEKLEADFHII
                     HGVRTRIQNTIRCVKELPLEVEDVVFSALASTQVVLTQHQKDLGALVMDIGGGTTDYI
                     LYVDSAVKQSGVLAVGGDHITNDISMGLRIPMARAEKLKIDEGSVTLGNCLPGETILL
                     KDESGFAGKEIERETLNTIIHLRLRETFELLKRLLEEEPYINYVGAGIFITGGCSLLD
                     GIEHLASEVFEMPAHVAHAQTISGLTSAFENPQFSTAIGLLRYAQAVQADRPRRGIRH
                     VIGKLFSRM"
     gene            11417..12814
                     /gene="ftsZ"
                     /locus_tag="VFO30_03195"
     CDS             11417..12814
                     /gene="ftsZ"
                     /locus_tag="VFO30_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9418321.1"
                     /translation="MIQLSKNYSLPEREEEVIPIKIIGVGGAGSNALDRIVLDGMDKA
                     DLIAINTDVQSLASSVAANKVQLGRSVTRGLGAGGDPDVGYQAAIESADEIRQALTDA
                     RMIFICAGLGGGTGSGAAPVVAQLAREAGSLVVALATLPFSFEGKRRGAQAQAALRQL
                     REITHAVICFENDRMGDMVAPKAGIHQAFATADITISQSVRSILNLLQRPGLIRIGFD
                     DLLSALRSENGRCLFGYGESDSDNRAHDALRQALKNPLMDKGKMLADAAHVLVQVAGG
                     PAMTLSEVEILMQEFGRYVSERTQIIFGTAVDSRMGNRLSVTIISSLAGEGENLSQQN
                     APAVAAAVVPAPPAWEAPSAETVDIVPEAPVAEPSEVMENLIPFEDTPPAAVAEPEIK
                     SAASAPRLIVPKQKPATLPSEPKAGKQKHAQAKQEVLQFEPVTRGRFERSEPTIVEGQ
                     DLDVPTFLRKNVRVK"
     gene            complement(12970..14346)
                     /locus_tag="VFO30_03200"
     CDS             complement(12970..14346)
                     /locus_tag="VFO30_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9418322.1"
                     /translation="MKLSSLSIWSIACRAVAVGVVGSILSAASLFAQQTPQSRADAEL
                     PSLLMIYKDLHIHPELSTHEERSAAIVAKELKAAGCEVTERVGKYDQPDKPCYGVVGV
                     MKNGTGPTVLVRSDLDALPVHEETGLPYASTVTTKNDEGKDVPVMHACGHDIHMSTLI
                     GTARALGKLKDKWHGTIVFIGQPAEETVGGAKAMLKDGLYTRWPKPDYALALHDNAEL
                     ETGKIGVTEGYALANVDSVDVTVHGIGGHGAYPHKTKDPIVLSAEIINAWQTIASREN
                     NPLDPVVITVGSIHGGTKHNIIPDEVKMQLTVRTYKKEVREKVLAAIERIAKQCGTTA
                     GLPQDKLPEVHVRQDEFTRATYNNPDLVKRVTPALKAAIGENNVVHKDPTMGGEDFCE
                     FSLPDNSLPAFMFVVGAVDPAKVAESNRTGTPLPSLHSSKFAPVPEPTIRTGIIGMTS
                     AVIELMKK"
     gene            14467..>14898
                     /locus_tag="VFO30_03205"
     CDS             14467..>14898
                     /locus_tag="VFO30_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418323.1"
                     /translation="MQFEKNTLLFGTDPTPRIVAVELGESGTVKVYWREKDGSIRVDV
                     EPFHPFVWADSDVVDLGLENAEKLAGDLKYNWLVTVDSWKELIALRNALKNSGRDFFA
                     FTDPVQHYLTATGRTLFKDLLFEELKRMQLEVLRFSEGEADH"
BASE COUNT         3078 a         4301 c         4437 g         3082 t
ORIGIN      
        1 gagagtgctg ccggtttggc tgccgcgccg ctcacgaaag cgcgtttgca gatcaaggaa
       61 atccgcggcg tgcaatttat cgacgacagc tacaacgcaa atcccgattc aatgaaagcg
      121 gcgctgcgca cgctggtcga gctggatacg gctggcaaac gtatcgcggt gctcggtgaa
      181 atgcgggagc tcggggagga atctgagcgc gcccatcgcg aaatcggtga gacggcggct
      241 gatctccgcg tcaatcagtt gatcgcgatc ggcgaagccg ccgccaccat cgtgaacgga
      301 gcgcgcgagg ctggcttgcg aaatgtcgcc actgcaaaat cgacaactga agccgcgcag
      361 ttgcttgcgg acgctgtggc gccgggcgac ctcgtgctgg tcaaaggaag tcgcgccgcg
      421 cggaccgaac tggtgatcga ggagtttgcg aagcgccagc cgacggaagg cgtaccgcca
      481 tgatgtatta cctgcatcgc ttcagcagtc agttcggcgg atttaatgtt ttcctttacg
      541 tcacgttccg tgccgttgcc gccgccgtga ccgcgttttt gatttcaatc ttgttcggca
      601 acttcgtcat tcgcaaactg acggcgctga agttgggcca gccgattcgg ctcgcaagag
      661 aagtgcatcg cctggctgaa ttgcatggcg gcaaacaagg cacgcccacg atgggtggcg
      721 tgctcattat cggttcggtt ttcatctcga gcgtactctg ggcgcgcctg ggcaatcgct
      781 tcgtcgcgct ggcgctcttc gcgatgcttt acctcggcgc cctcggcttt gtcgacgatt
      841 acttgaaggt caccaagaaa aaatccgctg gcgtgagcgg ccgctggaaa ttgctctttc
      901 aaatcgttct cgcggtcgtc attacaactg ttttcctgag cagccccttg ctcgaagtgc
      961 aggcgcgttc gctctacgtg cccttcgtga aagcgccggt agtcgacaat atgggctggt
     1021 tcacgtttct gttttttacg ctcgtcatcg ttggtgcatc gaacgccgtg aatttgaccg
     1081 acggactcga cggtctcgcc attggctgca ccgtcacggt ggcgctcgct tacgccgtcc
     1141 tttcctatgt cgcgggaaat tttcgcgtgg cagaatattt gcaggtcccg ttttaccctt
     1201 tcactgccga gctggccgtc gtttgtgcgg cgctcaccgg cgccggcttc ggctttctct
     1261 ggtttaactg ccatccggcg aaagtcttca tgggcgacac cggttcgctc gcgatcggtg
     1321 gattgattgg agtcgtcgcg atttgttgca agcaggaact gctgctcgtt gtcgtcggtg
     1381 gcgtgttcgt gatcgaagcg gtctcagtga ttctgcaggt gatcagcttt aaaacgaccg
     1441 gccgccgcat ctttctcatg tcgccactcc atcatcattt cgagctggcc ggttggaagg
     1501 aaaacacggt gatcgtgcgt ttttggatct tgtcgggaat atgcgcgctc ctcgggctcg
     1561 cgaccctgaa gttgaggtaa aagtttgcgc tacaaaaatc aacacgtcgc ggtcctcggc
     1621 gccggtttga gcgggacagc tgcggcgctt ctactcagat cggagggggc ggaagtcgcc
     1681 gtgctcgaca acgcagaaga aaagaatctt ctcaaatcca cactggagaa tctgcgcgcc
     1741 caaggggtgc gcgtgatctg tggaccggcg gcgaatgccg atcaaacgaa gtacgacttc
     1801 gcggtcctga gtcctggtat cgacccgctt tcgccgctcg cgctcaattt cagttcccgc
     1861 aagatcgaca ttaccggcga actcgagctc ggctggcgct cgtgcggact tccggtcatc
     1921 gccgtgaccg gcacgaacgg caagaccacg acgacagagt tgctcgcgca aatgctgaac
     1981 gcatgcggcc agcgcacgat cgcgtgcggc aatatcggca aaccgctttc cgaagtcgcg
     2041 cgcgagaaag agccgttcga tgtgctcacc gtggaagtga gctcttttca gctggaaacg
     2101 atcaaaactt ttcggccgtc catcgcgctc tggctaaatt tcgctcccga tcatctcgat
     2161 cgctaccgct cggtcgctga ctatcgcgcg gccaagctgc gcattttcga aaatcagacc
     2221 ccggatgacg tcgcgatcgt aaacgtgctc gataatttgc gggacttgcg cgcgcgcatc
     2281 atcagcttca gcgcctacag cgaccgcgcc gatttccgct tggccggcgg cgcaatcgtt
     2341 tacgaaaaca aaccggtcct gcggttgtcc caaaccaagc tgcgcgggtc gcacaacatc
     2401 gagaacctga tggcgacact tgccgcgggt atggcgcgcg gtctgtcgtt cgagcaaatg
     2461 gccgagccgc tctcggctta cgagccccgg ccgcaccggt gtgaattcgt taggcaaatt
     2521 gggggcatcg attacatcaa cgactcaaag gcgacgaatc tcgatgcagt cgagaaggca
     2581 ttgctcgcgc aaagcaaacc ggtcgtgctc atcgccggag gaaaagacaa aggattcgat
     2641 tttcagccgc tccgccgcgt tgtgaaagaa aaggtaagat cgaccgtgtt gatcggcgag
     2701 atggcggaac ggatcgcgcg tgattggagc ggggcggtta cgtcggagat agccggctca
     2761 cttgccgacg cgctcgagcg tgcgcgtgct gtcgcgaagc ccggggacgt catcctgttt
     2821 tctccgggta cgtcgtcctt cgacatgttc aagagctacg ccgatcgcgg cgaccaattt
     2881 cgcgcgctcg tgcaggcgct gccgcaacag ccatgaaact ctccagaatc ctgaagaaac
     2941 tccgcgccac gaccattcgc cgcccactgc gaacctccac gcaggcgaat tacgacgaca
     3001 tgtccgagcc gaacatgaag ctctcgcgcg ccttgctcgt cgttctgctt ctgcacgtcc
     3061 tcgccgtctc cggcatcatc gcgttcaacg caatcaagac gcggcagaca gccgctgctg
     3121 cttcgaccgc gtcgccgagc gcgacgccag cgacaaaacc atcgcaagtc gcggccgcgt
     3181 caacttccgc caccgccggt aaaactgagc acgcgaaaaa aggcgaaagc gtcaaattag
     3241 ccgccgattc gcacaaggtc tacgtcgtag ccaaaggcga taacccggtc accatcgcga
     3301 aaaagttcaa ggtctcctac gacgatctca tcgcgctcaa ccatatcgat gacccgcgca
     3361 agttgcgcgt cggccagaag cttttgattc caaccagaac gaccaaggcg aagaagaccg
     3421 ccgactagct gtcgctccca catgcatcgc aaaagcgctt acatcctttt cgtggccgtg
     3481 ctcgccatgc tcgtcattgg catcgtcatg ctttttagca caagtgcctt cgcccgcgac
     3541 agccacggca acatgtattt cttcattcgg cgccaggcga tctggctcgg gataggatta
     3601 acgctctgcg cggccgccgc tctgattgac tatcatttct ggcaacggac atggtggctg
     3661 tggtttagcc tcgccgccat cgcgcttgcg ctctgctttg tgccgcacgt cggaatgcgc
     3721 atcaatgggt cgcgccgctg gatcgctatc gcccagttgg ggttgcaacc ctccgaaatc
     3781 gcgaagatcg cggcggtgtt ttttctggcg gcgtggtttt cgcgtcacga gaacgcgagc
     3841 ggcacgattg tgtatggctt tgttttgcca ctgacaatca ctggcttgct tctcgcgttg
     3901 attctgtttg aagtcgacct cggcacgact atcctgctcg gcgccgcgac gctcgtcgtc
     3961 atgttcgtcg ccggagtaaa cccgatcctg ctcgggtcgg tctctctcgc cgggctcggc
     4021 gcaattcttt tgctcgcgac gcacatctcc gaacgaatgt cacggctttc tgcgtttctg
     4081 gatccgcagc ggttcaaaca agatgcgggc ttgcagcaaa tgcaggcgtt aatcgcgtgg
     4141 ggtagcggcg gcgtggaagg actcggcctc ggcaacggcc gccagaagat gctgtacctg
     4201 ccctacgcac acaccgactt catcttcccg attatcggcg aggagctcgg gctccgcgtg
     4261 agccttgccg ttatcttcct tttcgttctg atcatcattt gcggcatgat gatcgcgctg
     4321 cacgccaagg accgcttcgg tctgctcctc ggctgcggca tcgtttcgct cctgatcttg
     4381 caggcggcgg tcaacatcgg cgttacgaca tcgcttttgc cgaacaaagg cttgccgctg
     4441 ccattcatta gttacggcgg ctcgaatctg gtcgcctgcc tgttcggcgt aggtttgctt
     4501 ttgaacattt accggcaagg tgtgctcgag ccgaagcaga aaggtaccgc gctacaggcg
     4561 cgaacgacgc cgcgcatata accaagatcg acaatcgact ttcatgaacg cagtcatcga
     4621 aggtggatcg agctgtccga gcacgatgct acgaagctgc ggcgaagccg cgttatttaa
     4681 catcgcctgc ggagcggccg ttccaccttc gcgtcagttc atatgaatgc agtcatcgca
     4741 tgtggtggaa cgggaggaca tctcttccca ggcattgcgg ttgccgaggt gctggatcag
     4801 cgcggacatg aagtgatgct gttcgtctcg gaaaaggaga tcgacagcct cgcgctgtcg
     4861 acgcggtcgc agtttcgatt cgaaaaactg ccggttgtcg ggctgccatc gctttattcg
     4921 cccgcgatct ttggattcat ccgccgcttc agtgaaagcc tgtcacgctg tcgagcgatc
     4981 tacgaaaagt ttcaaccgca ggccgtgctc gggatgggcg gcttcacttc cacggcgcca
     5041 attctggctg gccgcctacg cggcgcctcc actttcatcc acgaatcaaa tgctgtcccc
     5101 ggcaaggcca atcgcatgac cgcgcggtgg gcgcgcgccg tcctgctcgg cttcaaagaa
     5161 tgcgctccat tctttccgaa agcgcgcacc gaagtcaccg gcacacccat ccgcactgaa
     5221 ttgcgacgct tggatcgacg tgttgcccgg caaaagctcg ggctgcgcga cgacctgccg
     5281 actttgctcg taatgggcgg gagccagggc gcgagcggga taaatcaggc gctgatcaaa
     5341 tcgctcgctt cgctgcgcga gatggcgcta caagtcattc atctctccgg cgcacgcgac
     5401 gaacgtcttg tcgccgacaa ttaccggcgc gaaaatattc ccgcttttgt cgcggcgttt
     5461 caccaaggga tggaggaagt ttacagtgcc gccgatcttg ccgtcgcgcg cgccggcgcg
     5521 gcgagtctgg ccgagctggc gtgttttgcg ctccccggca ttctcattcc gtttccctac
     5581 gcgactgacg atcatcagac ccgcaacgcc gagatcttcg cccgggccgg cgccgcgatc
     5641 ctgctcaagg agtcggagat tcaaggtgac agtctcgcgc gcaaaattaa ggaactaatc
     5701 gccgatgcga cgaagttgcg gcggatgtcc gagatttgtt cgcggcttgc gccgaaagat
     5761 gcggctgagc gcgtcgtggc cacgattgaa aaatatacca atgacggccg caattgatct
     5821 tgatctcgca acgtgcctca cgcgcgagcg gcatcgcgtg catctcgtcg gtgttgcggg
     5881 cagcggaatg agcggactgg ccggactgtt gctgcaactg ggtcatgaag tccgcgggtc
     5941 cgataaagtg acgacgctgg agacggagcg cttgcagcgg ctcggcttgc aatttcacga
     6001 gaaacagcac gccgacgaag cgcacgacgc tcagctcatg attttttcct ctgccatcaa
     6061 gttcgacaac gcgattctgg ctaatgcgcg cgccgcggga ataccaatcg tccgccgggc
     6121 tgaagcactt gccgccatca tgtcatgcaa acgcgggatt ctcatcgtcg gtatgcatgg
     6181 gaaaacgacg acctcggcga tggccgcgca cgtcttgcgc gaaggcggtt tacatccgtc
     6241 gcattacgtc ggcgcggaaa tcccaatcct gggcacgaat gcgcattggg acgcgcgcgg
     6301 cgagtacttc gtggccgagg gcgatgaaag cgacgggaca ctaaagtatt tccggccgga
     6361 gcacacgctc gtacttaata tagaggccga gcacctcgac ttttaccccg accttggcgc
     6421 aatcgagaaa gcgttcgtcg aactgatcgg ccagaccagc ggcaaaatct tctacagcgc
     6481 cgacgacccg attgcgaccc gaatctgtcg atcttgcacc ggagcgattg gcttcgggtt
     6541 gtctctggat gccgagtacc gcgcggcaaa catcaggctg caggattttg tctccacgtt
     6601 ctacatttac cgtcgaagcg agcgcttggg ggaagccgtg ctaaatgttc cgggcgagca
     6661 caatgtgcac aacgcgctcg gggtgattgc gctcgcaagc gaactcgatg tgccattcgc
     6721 gaaaatcgcg agcgcgctcc gcaagtttca gcacgcgcgc cgccgtttcg aaatcaaata
     6781 cgccagcgac cggttcctgc tcgtcgacga ttacggccat catccaaccg aaattcgcgc
     6841 cacgttaaaa acggcacgcg caatccgacg caaacgagtg ctgacgatgt tccagccgca
     6901 ccgttactcg cggacgaaag cgctcgccaa agaatttggc tgcgcgttcg atgatgctga
     6961 tcgcatcgtc gtaacggacg tttaccccgc gagcgaagcg ccaatggcgg gaatcagcgg
     7021 tcaaaccata gtggatgaaa tcgtggcgca cgggcatcgc ggcgtgactt accagccgcg
     7081 gctcgactcg gtccatcgcg acatcgggaa catgctcgat tctggcgacc tcgtgctgag
     7141 tctcggcgcg ggcgacattc acgagcaatt gtcgatctta gccgccgacc tggtggtcgc
     7201 ggagaaagtc aaagagatag tcggcgacga aggtgacgtg cggctttacg agccgctgtc
     7261 acgacacacg accttgcgcg tcggcgggcc ggcgcaattc tgggtcgaac cacgcaccga
     7321 aatagctttc gccgagctaa tccggttcgc tcggagggaa aatcttccgc tgttcgtcat
     7381 cggtcgcggg tcgaacctgc tcgtgcgcga cggcggaatt cgcggcctgg tcgttcatcc
     7441 cagtggcggc gaattcgaca agatcgacat caatgggcag gagatcacgg ctggcgtcgg
     7501 tgtgaaaatg aaagaagtcg cttacgcggc acggtctgcg ggacttgggg gactcgaatg
     7561 gatggaaggc attccgggcg cggtcggcgg cggactgcgt atgaatgctg gcgcgatggg
     7621 cgcgcaattg tgcgagaacc tggtgcgcct ccgctatctc gatgccgagg gaaatcggca
     7681 cgtgcaagat cgacatgagc tcgatctgcg ctaccgcgat tttccgttgc tgcacaacaa
     7741 ttacgcgatc tcggcgacat tccgcggcgt tccggccccg cgcgagcaga tcgaggcgcg
     7801 tctgcaggaa tcccaagaga aacgacgcac gactcagcca atcgcgaaaa gcgccggttg
     7861 cattttcaaa aacccggaca gcagtcccgc gggaaaattg gtggaggaac tcggcttgaa
     7921 gaactcacgt attggcaatg cgcgcgtgtc ggaggtgcac gggaatttca tcgtcaacga
     7981 cggcggcgcg acggcggcgg agatgctgga attgatcgac cgaatcaaaa cggccgcgct
     8041 aacgaaaggc ggcattcagt tggaaacaga agtgcaaatc gtcggagaaa tggcttgatg
     8101 gaagtgccga aaaaatttgc ggtattgatg ggcggccccg gatctgagcg cgacgtgtcg
     8161 cttgcgaccg gacgcggtgt ggccaaggcg ttgcgttcgt taggcgtgga agtcctcgaa
     8221 ctcgacgtgc gcgacggaaa cttcaccctg ccgtcagatg tcgatcttgc ctttcttacc
     8281 atccacggaa cttttggcga agacggtcaa ttgcagcgga ttctggaaga gcgcggcgtc
     8341 gcctacacag gcgacggcgt agaaccaact gcaactgcga tcgacaagat ccggtcaaag
     8401 cgaaagtttg acgagcacgg cgttgcaacc ccgcgatggg aagtgattca accaaaccag
     8461 cggccatcca tttcgctacc catcgtggta aaaccgccgc gggaaggctc taccgtcggg
     8521 gtgatcatcg ttaagaacga acgcgagcta gaatcggccg tccgcgaggc ggcaaattac
     8581 gaccgagaat tgctggtcga aaaatttgtg cccggtcgag aattgacgat tggtatcctc
     8641 ggtgatcaag ccctgccgat ccttgaaata attccaaagg gcgggtttta cgatttcaaa
     8701 aacaagtacc cgtttctgaa tccgcaggcc ggcggaggcg ccgagcatgt ttgtcccgcg
     8761 aagatcgaca aagacaaaac gcgccagatt caggagctgg cgttgcgcgc attccgtgca
     8821 ctggggcttc aagtttatgg acggattgat gtgattttgt cagaccgcgg cgaaccgttc
     8881 gttctcgagg tcaacacgat ccccggaatg actgaggcca gcttgttgcc ggaagcagcc
     8941 gcggctgcgg ggatcagcta tatcgatctt tgcctgcgca tcatcgagct ttccagggca
     9001 agaatggaga agaaggcgcg atgaggaacg ggcgcgcccg gccgcgcaat cgacgagtct
     9061 cgaactcgcg tcaacgccgg cggcaacatt tgctcgacgt gaaggtacgc tcgcgcacgg
     9121 cgacgcagca ccgcacgcgg cgggtggtcg tgcttgtttc gaagatcacg ctcgcccttg
     9181 ggctgtgcgt cgggatttat ttcggcgtcc gcgaaggcgc gcggcggctt tttttcgaga
     9241 acgccgatta ccagctgagg gcgattgagg tgcagacgga cggcacgttg gcgcgcgacc
     9301 agatcctgca gatcgccggg ctacgcgagg gcgaaaatat tttctcgata aatttggcga
     9361 aggtgcatga cgacttgcaa cagctcccgc aggtcgacga tgtgcaggtc gtgcgcaagc
     9421 tgccggccga gattgatatc aaggtcaccg aacgtaaacc ggtcgcatgg atcaccgagg
     9481 aaaaggaact ctccgacccg ttcaatgctg acagcgcctt tcttgttgat gggcgcggcg
     9541 ttttgatgaa gcaaaagaaa ctcttgccgg agtatctcgg cctgcctctc atcgtgggct
     9601 gcatcggcga atcgtttgag gctggcaacg tggtcgagtc acctgaagcc aaggccgccc
     9661 tcgaactgct gcggctgagc acgcgcagtt tcatgcaaac gcgctttcag gtgcgcgaga
     9721 tcgacatttc aaagggctac tgccttgtcg tgacagataa gaaccacacc cgcgtcacgt
     9781 tcgctttcga caaactcgag gagcagttgc agcgactgga gcagtttctg gtttactccg
     9841 atgacacgaa acgcgagctc gcgacggtga acctgcttgt gcagcgaaac attccagtca
     9901 cattcgcgaa accggcggcc gaagtgatca acgatacgat ttacccagaa gcgacaccgc
     9961 gcattttgaa agcgataccg gtccgccccg ctcgcgccgg tgagtccgtc ccggcacggt
    10021 caaaatcgac atccacaccg tcggtgcggc gcgcaattcc ggttgcgcag gaaaagaaga
    10081 attaaatatg gcaagcggcg atttaatggt cggactcgag attggcacgt cgaaggtttg
    10141 tgtcgtggtt ggcgaaggtc gtcctgacgg cacgctcaag attctgggcg ttggccaggc
    10201 gccgtcccgc ggaatccgca aaggcgagat tgtcgatttt gaaacggcga tgaaatgcgt
    10261 gcacgaagcg gtggtcgatg ccgaacagaa gagcgatgtg atgattcgca gcgtctacgt
    10321 cgctgtggcc ggtgcgcaca ttcaaagttt caacaaccgc gggtgcgtcg ttctgccgga
    10381 ggaccgcgat gagatggatg agcaggatat cgaagatgtg aaagtgaacg cgcgcgaagt
    10441 cagcatacct gcgcaaaacg cctttctcca ttcgatcatt cagcattacc acgtcgatgg
    10501 tcaggatggc gtgctcaacc cggtcggcat gctcggcgaa aaactcgagg ccgattttca
    10561 catcatccac ggcgtgcgca cgcggattca aaacacgatc cgctgcgtaa aggagttacc
    10621 gctcgaagtc gaagacgtgg tgttcagcgc gctcgcttcc acgcaggtcg tgctcacgca
    10681 gcaccagaaa gatctcggcg cgctcgtcat ggacatcggt ggcggcacga ccgattacat
    10741 tttatatgtc gacagcgcgg tgaaacagag cggcgtcctt gcggtcggcg gcgatcatat
    10801 caccaatgac atctccatgg gactgcgcat cccgatggcc cgcgccgaga aattaaaaat
    10861 cgacgaggga agcgtcacgc tcggcaattg tctgcccggc gagacgatcc tgttgaagga
    10921 cgagtccggt ttcgcgggca aggagatcga gcgcgagacc ttgaacacaa tcattcacct
    10981 gcgcctgcgc gagaccttcg agctgctcaa acgcctgctc gaggaggagc cgtacatcaa
    11041 ttacgttggc gccggcattt tcattaccgg cggctgcagt ttgctcgatg gaatcgagca
    11101 tctcgcatcg gaagttttcg agatgccggc gcatgtcgcg cacgcgcaga ccatttccgg
    11161 gcttacgtcg gcgttcgaaa acccgcagtt ttccaccgcg atcggtttgc ttcggtacgc
    11221 gcaagccgtg caagccgatc ggccgcgacg cggcattcgg catgtgatcg ggaaactttt
    11281 cagcagaatg tgatgaatca ccaaatgcct ccgcaattgt catgtcgagc ggagtcaaaa
    11341 catctcttac agttgatgag agatccctcg acttccctcg ggatgacaga tcactgcatg
    11401 tgaaggaaag atcgacatga tccaactcag caagaactac agtttgccag aacgggaaga
    11461 ggaagttatc ccgatcaaaa tcatcggcgt cggcggcgcc gggtcgaacg ccctcgaccg
    11521 gatcgtgctt gatggcatgg acaaagccga cctgatcgcc atcaacaccg atgtgcaatc
    11581 gctcgccagc tcggtcgccg ctaacaaggt gcagcttggt cgcagcgtca ctcgtgggct
    11641 cggcgcgggc ggcgatcccg acgtcggcta tcaagccgcg atcgaatcgg ccgacgaaat
    11701 ccggcaggcg ttaacggacg cgcggatgat cttcatctgc gccggccttg gcggcggcac
    11761 gggttccggc gcggcgcccg tcgtagcgca gctcgcgcgc gaagccggct cactcgtcgt
    11821 cgctttggcg acgttgccgt tttcattcga aggcaagcgg cgcggcgcgc aagcacaggc
    11881 tgcgctgcgt caactccgcg aaattacaca cgcagtcatc tgtttcgaga acgaccggat
    11941 gggggacatg gtcgcgccga aagcggggat tcatcaagcg tttgccaccg cggatatcac
    12001 catcagccaa agtgtccggt cgattctgaa cctgcttcaa cggccaggcc tcattcgcat
    12061 cggtttcgac gatctgctta gcgcgttgcg cagcgaaaac gggcggtgtc ttttcggtta
    12121 cggcgaatct gattcggaca accgcgcaca cgatgcgctg cggcaggcgc tgaaaaaccc
    12181 gctcatggat aaaggaaaaa tgctggccga cgcggcgcat gtgctggtgc aagtcgcagg
    12241 cggtccggcc atgactttgt cggaagtcga aatcctgatg caggaattcg gtcgctacgt
    12301 gagcgagcgc acccagatta ttttcggaac ggcagttgac agccgcatgg gcaaccgttt
    12361 gagcgtgacg atcattagtt cgctcgcggg agaaggggaa aacctgtcgc aacaaaacgc
    12421 accggctgtg gcagcagccg ttgtgccggc tccgcccgct tgggaagcac cgtcggccga
    12481 aacggtcgac atcgttccag aagcgccggt cgccgagcca tcggaagtca tggaaaatct
    12541 catcccgttt gaggacacac ctccagcggc cgttgctgag ccagagatca aatccgctgc
    12601 ctcagcacca cggctgatcg tgccaaaaca aaaaccggcg acactcccaa gtgagccgaa
    12661 agccggaaaa caaaaacacg cgcaggcgaa acaggaagta ttgcagttcg aaccggtgac
    12721 gcgggggcgc ttcgaaagaa gcgagccgac cattgtggaa gggcaggact tagatgtgcc
    12781 gaccttccta cgtaagaacg tccgcgtgaa ataacatcgc gcggcttgtg ccacgctgtc
    12841 atcccgaacg aatgtgaggg acctcccaca aacatcctga tcacacgaat tcttcggagt
    12901 gtgatccatc agccaatgtg ggatccctca gcgtctgcgc gcttcgggat gaccacgttg
    12961 tgtggcgcgt tatttcttca ttaactcgat cacggcgctt gtcatgccga taataccggt
    13021 gcgaatcgtc ggctctggca cgggcgcgaa cttgctcgaa tgcaaactgg gcagcggcgt
    13081 tccggttctg ttcgattcgg cgactttggc cggatcgact gcgccaacga cgaacatgaa
    13141 cgccggaagc gaattgtccg gcaggctgaa ttcgcaaaaa tcttcgcccc ccatggtcgg
    13201 atctttgtgg acgacattgt tttcgccgat ggcggctttg agcgcgggcg tgacgcgttt
    13261 cacgaggtca ggattgttgt aggttgcccg cgtgaactcg tcctgccgga cgtgcacctc
    13321 aggcaatttg tcctgtggca agcccgccgt ggttccgcat tgcttggcga tgcgttcgat
    13381 tgccgcgagc actttctcgc gcacctcctt tttgtatgtg cgcacggtca actgcatttt
    13441 cacttcgtcg ggaatgatgt tgtgcttggt accgccgtga attgatccga cggtgataac
    13501 caccgggtcg agcgggttgt tttcgcgcga ggcgatcgtt tgccacgcat tgatgatctc
    13561 ggccgaaaga acaattgggt ctttggtctt gtgcggatac gcgccgtgtc cgccgatgcc
    13621 atgcaccgtc acgtccactg aatcgacatt ggcgagcgca taaccttcgg ttacgccgat
    13681 cttccccgtc tcgagctcgg cattgtcatg cagggcgagc gcataatccg gttttggcca
    13741 gcgcgtgtaa agcccgtcct tcagcatcgc cttcgcgccg ccgactgtct cctccgccgg
    13801 ttggccgata aacacgatcg tgccgtgcca tttgtccttc aatttgccga gcgcgcgcgc
    13861 cgtcccaatg agcgtggaca tgtgaatgtc gtgcccgcag gcgtgcatca ccggcacgtc
    13921 tttgccttca tcgtttttcg ttgtgacggt actggcatag ggcagcccgg tttcttcgtg
    13981 taccggcaac gcgtcgaggt cgctgcgcac cagcacggtc ggcccggtgc cattcttcat
    14041 tactccgacg acgccgtagc atggtttgtc gggctggtcg tatttaccca cgcgttccgt
    14101 cacttcgcag ccggcagctt tcaattcttt cgcgacgatc gcggcggaac gttcctcgtg
    14161 cgtggacaat tcgggatgga tgtgcaggtc cttataaatc atgagcagtg atggcagctc
    14221 cgcgtcggcg cgcgactgcg gcgtttgctg cgcgaaaagc gaggcggcag acaagatcga
    14281 cccgactacg ccgacggcta cggcgcggca ggcaatcgac cagatcgaca atgaagagag
    14341 tttcattccg cagttataac aggccgacgt tgtagggcaa ccgctgcggt tgccgactta
    14401 aaaacggcag gcgacgcgcc taccctacaa tttgtcattc cgagcgaagt cgaggaatct
    14461 ctcactgtgc aattcgagaa aaacaccttg ctcttcggca cggatccgac gccgcgaatc
    14521 gtcgcagtcg agctcggcga gagcggcaca gtcaaggttt attggcgtga aaaggacggc
    14581 tccataaggg tggacgttga gccgtttcat ccattcgtct gggccgacag cgatgttgtc
    14641 gatcttggat tggaaaacgc agagaagctt gcgggtgatc tcaaatacaa ctggctagtc
    14701 acggtcgata gctggaagga actgatcgcg ctgcgcaacg ctttgaaaaa ttccggacgc
    14761 gatttcttcg ctttcaccga tccggtacag cactacctca ctgcgactgg gcggacgttg
    14821 ttcaaggatc tgctgtttga agagctcaaa cgcatgcagc tcgaagtgct gcgtttcagc
    14881 gaaggcgagg ccgatcac
//
LOCUS       DASTDG010000101         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_190765, whole genome shotgun sequence.
ACCESSION   DASTDG010000101 DASTDG010000000
VERSION     DASTDG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190765"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VFO30_03210"
     CDS             complement(<1..1715)
                     /locus_tag="VFO30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418324.1"
                     /translation="MTETELSSTEETVAIPKRGTFIERMIEGSTRNKFLVFIFTLFAV
                     AAGIYGLMHTPLDAIPDLSDVQVIVYTDWEGRSPDLVEDQVTYPISTLFIAAPKVKFV
                     RGESMFGKSFIYVIFEDGTDIYWARSRVIEYLNSVRGSLPEGVNPVIGPDATGVGWVY
                     EYALVDDSGKHDLAELRSIQDWNLRYALSSVKGVSEVAPVGGFVKQYQVDLDPNALVA
                     YHVPLSDVVNAIKMSNADVGGKIFEVATTEYYIRGRGYIKKIEDIENIVLKVENGTPV
                     YLKSVGNIHLGGDIRRGVAELDGRGEVVGGIIVMRYGENALNVIDGVKKKLEEIKGSL
                     PEGVHVVTTYDRSDLIERSIATLREKLIEESVVVALVCLVFLWHVRSALVAIVTLPIA
                     IILSFVPMWWMHVTSNIMSLGGIAIAIGAMVDSAIIMIENAHKALENRVTRASGLRSG
                     EDASGTLALPFAERTRIIVDAAKGVGRSLFFALLVITVSFVPVFSLTAQEGRLFRPLA
                     FTKTFSMFFASFLGVTLVPVLMTLLIRGKITPEEKNPVNRFLIRAYQPFADFVLRYRW
                     FTLGCA"
     gene            complement(1712..3070)
                     /locus_tag="VFO30_03215"
     CDS             complement(1712..3070)
                     /locus_tag="VFO30_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9418325.1"
                     /translation="MNIRLIVTALVLLTSLGAASCSKAPSAKKDSNIDYWTCTMHPSV
                     HSKDPGKCPICSMDLVPVMKKGGAEAQTSQTQGEQMKGGGGETKGMPGMPGMKAGEMK
                     GGEKPGEFVVPVERQQQIGVRYAKVERKPLRHTIRAVGLIVPDRSRNWQFVSRVDGYV
                     QRLNVTSPGELVEKDAPLMSIYSPDLLTSEREFVELLRMRDEAKTKDARETPQRLIES
                     AKRRLQLWNVTSEQVAELERTRKASDTLTLLSPFRGVVQSVPVEQGKSVKLGDMLVEV
                     ADLSVVWMWAEFYENELSMLQVGQKLSITAKSYPGQNFDGTLALINPFLDETKRTAKV
                     RIDIPNPDFKLRPGMYVNAELEMDMGAALTIPVSAVMPTGMRSVVFVDKGEGKLEPRI
                     IQLGAKYGDIYEVQSGLQENEPVVASANFLIDAESKVQGALKEFDEGEKAQETAAPGK
                     KP"
     gene            complement(3072..>4313)
                     /locus_tag="VFO30_03220"
     CDS             complement(3072..>4313)
                     /locus_tag="VFO30_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9418326.1"
                     /translation="SLGEVTAAVLANNPAIKESLRKWKAARARVPQAAAWDDPRLGGD
                     SRVHRFVDVPPNAFMDQSISIEQIVPVTGKNLLRARAALADALSAYEDVRRQQLDVIA
                     AARGSFFRLANAYEQLEINRKNIESLKQIADISRAKYEAGTQTAADVLVAETDESKLE
                     EARRDLERQLSDEQAQLNTLMNRDAFAPLGQPATASVQMPELSPTALRPLVLTQRPEI
                     KMAEAKIDMEKSKLQLARREWIPDPALMAKAQRYNDAGQAVSELDAGISFTVPWINFR
                     KYAAGVREAGENVGAAEQALDRSQKEALRLLRDQLEKIETFHHHVELFRDKLVPQAQQ
                     AFEANRLAYESGKVSFLDWITAQRVLREIEATARMHLTDYQVAVAELEAIIGAQVYPL
                     PNTEVETSNGGIGKSAKQRGR"
BASE COUNT          847 a         1270 c         1200 g          996 t
ORIGIN      
        1 gcacagccga gcgtgaacca gcgatagcgg agaacgaaat cggcgaaggg ctggtaagca
       61 cggatcagga aacggttcac cggatttttc tcctccggcg tgattttgcc gcggataagc
      121 agcgtcatca gcaccggcac gagtgtcacg ccgagaaacg acgcgaagaa catcgagaat
      181 gtttttgtga aggcgagcgg ccggaacaac cggccctcct gcgccgtgag agagaaaacc
      241 ggcacgaagc ttaccgtgat cacgagcagc gcgaaaaaga gcgagcgccc gacgcctttt
      301 gccgcgtcca cgatgatgcg agtgcgttct gcaaaaggta gcgcaagcgt cccgcttgca
      361 tcttctccag atcgcaagcc ggaggctcgc gttactctat tttcgagcgc cttgtgcgca
      421 ttctcgatca tgatgatcgc cgaatcgacc attgcgccga tggcgatcgc gattccgcct
      481 aacgacatga tgttcgaggt gacgtgcatc caccacatcg gcacaaacga gagaatgatc
      541 gcgatcggca gtgtgacgat cgcgacgagc gcggagcgga cgtgccagag aaagacgagg
      601 caaacgagcg cgacgacaac gctttcttcg atcaacttct cgcgcagcgt cgcgatcgag
      661 cgttcgatca aatcgctgcg atcgtaggtt gtgactacat ggacgccctc gggcagcgac
      721 cccttgatct cttccagctt cttttttacg ccgtcgatga cattgagcgc gttctcgcca
      781 taacgcatga cgatgatgcc gccgacgact tcgccgcgac catcgagttc ggctacgccg
      841 cggcggatgt caccaccgag atgtatgttt ccgacacttt ttaagtaaac cggcgtgccg
      901 ttttcgactt tgagaacaat gttctcgatg tcctcgatct ttttaatgta gccgcggcca
      961 cgaatgtaat actcagttgt agcgacctcg aaaatttttc cgcccacgtc ggcgttgctc
     1021 atctttatcg cgttgacgac gtcgctgagc gggacgtggt aggcgacgag cgcgtttgga
     1081 tcgagatcga cctggtattg cttcacgaag ccgccaaccg gcgcgacttc ggacacgccc
     1141 ttcaccgagg agagggcgta acgcagattc caatcttgga tcgagcgaag ctctgcgaga
     1201 tcatgtttcc cgctatcgtc aacgagcgcg tattcataaa cccagcccac cccggtcgcg
     1261 tccgggccga tgacgggatt gacgccttcc ggcagcgagc cgcgcacgga gttgaggtat
     1321 tcaattacgc gcgaacgtgc ccagtaaata tccgttccat cctcgaagat gacgtagatg
     1381 aacgatttgc cgaacatcga ttcgccgcga acgaatttca ctttgggcgc ggcgatgaag
     1441 agagtggaga tcggataggt aacctgatct tctaccagat cgggcgagcg accttcccag
     1501 tccgtgtaaa cgatgacctg cacgtcgctg agatccggaa ttgcgtcgag tggggtgtgc
     1561 atcagtccgt aaattccagc cgcgactgca aaaagcgtga aaatgaaaac gaggaatttg
     1621 ttcctcgtgc tcccttcgat catccgttcg atgaacgtgc cgcgctttgg aatcgcgacg
     1681 gtttcttccg tcgagctcag ttccgtttcg gtcatggctt ctttccaggg gccgcggttt
     1741 cctgagcttt ttcaccttcg tcaaactctt tgagcgcgcc ttgcactttt gactcggcgt
     1801 cgatcaagaa gttcgcgcta gcgactacgg gttcattttc ctgcaaaccg ctttgcactt
     1861 cgtagatgtc accgtatttc gcaccgagct gaataatgcg cggctcgagt tttccttcgc
     1921 ctttatccac gaacacaaca ctgcgcatgc cggtcggcat cactgcgctc accggtatcg
     1981 tgagcgccgc gcccatgtcc atttccagct cggcattgac gtacatcccg ggccgcagct
     2041 tgaaatcggg attcggaata tcgatgcgca ccttggccgt gcgttttgtt tcatcgagaa
     2101 acggattgat cagggctaga gtgccgtcga agttctggcc aggatacgac ttcgcggtga
     2161 tggagagctt ttggcccact tgaagcatcg acaactcgtt ctcgtaaaat tccgcccaca
     2221 tccagacgac gctgagatcg gcaacttcga cgagcatgtc acctagtttc acgctctttc
     2281 cctgttcaac gggcacggat tgcacgacgc cgcgaaacgg agagagcaaa gtgagcgtgt
     2341 cggaggcttt gcgtgttctt tccagctcgg caacttgttc tgacgtcaca ttccaaagct
     2401 ggagccggcg tttggctgat tcgatcaggc gctggggcgt ttcgcgcgcg tctttcgtct
     2461 tcgcttcgtc gcgcattcgc aatagctcga cgaactcgcg ctcgcttgtg agcaggtctg
     2521 ggctgtagat cgacatgagc ggcgcgtctt tttcaacgag ctcgcccggc gacgtgacgt
     2581 tcaacctttg cacgtaacca tcgacgcggg agacgaactg ccagttgcgg ctcctatcgg
     2641 gcacgatcaa gccgactgcg cggatagtgt gacggagcgg tttgcgctcg acctttgcgt
     2701 agcgcacacc gatctgctgt tgacgctcaa cgggcacgac aaactcgccc ggtttctcgc
     2761 cgcctttcat ttcgcccgct ttcatccccg gcattccagg cattcccttc gtctcgccac
     2821 caccgccctt catttgttct ccctgagtct gcgatgtctg ggcttcggcc ccgcctttct
     2881 tcatcaccgg cacgagatcc atcgaacaga tggggcattt gccgggatcc tttgaatgca
     2941 ccgacgggtg cattgtgcag gtccagtagt cgatgttgct atccttcttc gccgacggcg
     3001 ctttcgagca cgaagcagcc cctaacgaag tcagcagcac caaagcggtc actattagtc
     3061 gtatgttcat ttcatctgcc tctctgtttc gccgatttgc cgattccgcc attcgacgtt
     3121 tcaacctctg tatttgggag cgggtaaact tgcgcgccga tgatcgcctc cagttccgcg
     3181 acggcgacct ggtaatccgt caggtgcatg cgggcggttg cttcgatctc gcgcagaact
     3241 cgttgcgcag tgatccaatc gaggaacgac accttgcccg attcgtaggc gagccgattg
     3301 gcttcgaagg cttgctgcgc ttgtggcaca agcttatcgc ggaacagctc gacgtgatga
     3361 tgaaacgttt cgatcttctc gagttgatcg cgcaaaagcc gcagcgcttc tttctgtgaa
     3421 cgatcgagcg cttgttcggc ggcgccgacg ttttcaccgg cttcgcggac tccggcggcg
     3481 tattttcgaa agttgatcca cggaacggta aaggagatac cggcgtctaa ttcgcttacc
     3541 gcttgcccgg catcattgta acgctgcgct ttggccatca aggccggatc gggaatccac
     3601 tcgcgacgcg cgagctgaag cttcgatttt tccatgtcga tcttcgcttc cgccattttg
     3661 atttccggtc tctgcgttag gaccagtggt cgaagcgcag ttggcgagag ttccggcatt
     3721 tgaacgctcg ctgtcgccgg ttgaccgagc ggcgcgaacg cgtcgcgatt catcagcgtg
     3781 ttaagctgcg cctgctcgtc cgaaagctgg cgctcgaggt cgcgccgcgc ttcctccagc
     3841 ttgctctcat cggtttccgc gacgagcaca tcggccgcgg tctgtgtgcc cgcctcgtat
     3901 ttggcgcggc tgatatcggc aatctgcttc agggactcaa tgttcttgcg attgatttca
     3961 agctgctcgt aagcattcgc gagccggaaa aacgatccgc gcgctgcggc gatcacatcc
     4021 aattgctgcc gccgaacatc ttcgtacgcc gaaagcgcat cggccaaagc cgcccgcgcg
     4081 cgtaagagat ttttgccagt aaccgggacg atctgctcga tgcttatcga ttggtccatg
     4141 aacgcattcg gcggcacatc gacaaagcgg tgcacccgtg aatcgcctcc taatcgcggg
     4201 tcatcccatg ccgccgcctg cggaacgcgc gcgcgcgcgg ccttccattt gcgcagcgat
     4261 tctttgatcg ctggattgtt cgcgagcaca gcagcagtca cttcgccgag cga
//
LOCUS       DASTDG010000102         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_926102, whole genome shotgun sequence.
ACCESSION   DASTDG010000102 DASTDG010000000
VERSION     DASTDG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926102"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFO30_03225"
     CDS             complement(<1..587)
                     /locus_tag="VFO30_03225"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HET9418327.1"
                     /translation="MMKSSLPWFENRFPLYLAPMAGVSDKIFRQLCKEYGADVLTTEF
                     VSAEGIFRRNQRTREYLDFDEIERPIGVQLFGGDAEHMAEAARQVVDLVRPDFVDLNF
                     GCPVNKVVCKNGGSALLKDYGTLSRVADAVVRAIAPLPVTAKIRIGWDADSINAVQVA
                     QILKNCGIAALTVHGRTRAQGYSGSADWDVIGEVAA"
     gene            complement(709..1212)
                     /locus_tag="VFO30_03230"
     CDS             complement(709..1212)
                     /locus_tag="VFO30_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418328.1"
                     /translation="MNPWPNEWAIKHRADRCAVTQRPFTPGEYFYTLLFHDADGYRRE
                     DLSEDAWTNRNENIQPFSFWKTRYEPLPEPPPEPLAKETAEQLFRRLIASPNPPANTC
                     YVLAAMLERKRTLKQIKSEARDSGRVLIYEHGATGDVFIVPDPQLRLDELEDVQNEIA
                     QLLRSAA"
     gene            complement(1209..2348)
                     /locus_tag="VFO30_03235"
     CDS             complement(1209..2348)
                     /locus_tag="VFO30_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9418329.1"
                     /translation="MRLLDRYVIRNFVQAYFYCIAGFISIWLIFDISDNISTLLDERV
                     GLGLAVQYYLTQVPQVFIILLPVSLLLALLFSLGRMSRANEIVSMLTAGISLPRILLP
                     LVIIGLLTVGATMALNYSLAPHAELVRKNFFSEARGRPEALVEGQIFRNRTDARTWFI
                     QNFRNGSNEFNNVQVLQQDSNDDIVTNYLASRAVYHAESQSWELSNAKVVRYDASGNV
                     IDEQFYPSVIVQHWSETPFRLGSANARAEYLSLPELRDYLHFNADFPATLLAPFRTHF
                     QYRLALPWTCFVVVCIAAPLGIGYSRRGVLASVASAVILVFSMNFLTHLFLALGEGDR
                     ISPLLAAWTPNVLFTVIGLYLFYLRASNREALGFRLFQPRRIVAR"
     gene            2371..>3996
                     /locus_tag="VFO30_03240"
     CDS             2371..>3996
                     /locus_tag="VFO30_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9418330.1"
                     /translation="MIALKDSLGTEFVERVRAIFSENGLLSKARNFEFRLQQQEMAAA
                     VAHALEEDRHLVVEAGTGVGKSLAYLVPAILFALEQHKKAIVSTHTINLQEQLVHKDI
                     PILKKVLPVEFEAALMKGRQNYICGRRLERALQSANELFTGPEQTELQRLAQWAATTR
                     DGSLSDLSIEPDPKVWMQVCSEAHICTQKTCGQNPRCFYQQARKRLLAADVIVLNHTL
                     LFMLLGSPEMQEERESGFLFPNDFLIFDEAHTVEQVASRQIGIGVSQYGLRSTIQRLY
                     NARTRKGLFTVTRDAAGVRLAAELVDDVNKFFASVDSKSDFRKGREFRVRDVGLVPDT
                     ITGRLTALQARIIDVAKRADDELLKAELQEFGRRIRDARDGIAIFLEQSAREHVYWVE
                     RTGKTERYLSLNAAPVDLAPVLRRMLFRENACCIMTSATLAVGRPDLAYFRERIGATD
                     AEPLQLGSPFDFQKQTKLFVVQKMPDPRDADYHRALERWIAHFVEKTDGRAFVLFTSY
                     RDLQQVAGEMQKFFVGKGMNLLVQGGGAPRGKLL"
BASE COUNT          861 a         1173 c         1190 g          772 t
ORIGIN      
        1 gcggcgactt cgccgatcac atcccaatcg gcgctgccgg agtaaccttg cgcgcgggtg
       61 cgcccgtgca cagtgagcgc cgcgataccg cagttcttca gaatttgcgc gacctgtacc
      121 gcattgattg agtcggcgtc ccaaccaatg cggatttttg ccgtgaccgg caacggtgca
      181 atcgcgcgga caactgcatc cgcaacgcga ctcaaagtcc cgtaatcctt cagcaaagcc
      241 gagccgccgt ttttgcacac gactttgttg accgggcagc cgaagttaag atcgacaaag
      301 tccgggcgca ccaagtcgac gacttgccgc gccgcttctg ccatgtgctc cgcgtccccg
      361 ccgaaaagct gcacgccgat cgggcgttcg atctcgtcaa agtccagata ctcgcgcgtg
      421 cgctggttgc gccgaaagat tccttcggcc gagacgaact ccgttgtcag aacatccgcg
      481 ccatattcct tacagagctg gcggaagatt ttgtccgaca cgccggccat cggcgcgaga
      541 taaagcggga atctgttctc gaaccaaggc aacgaagatt tcatcattcc attctctcac
      601 agaatcgttt gcgccgcagg tggatcgagc tctccgagct cgatgttaat ccaacgcagc
      661 cgcaggctgc ccattattta gcatcgcccg cggagcggcc gattcacctt acgcggcgct
      721 gcgcagcaat tgcgcgattt cgttttgcac gtcttccagc tcgtcgagcc gcagttgcgg
      781 gtcgggcacg ataaagacgt cgcccgtcgc accgtgctcg taaatcaaga cgcgcccgct
      841 gtctctcgct tcactcttga tctgtttcaa agttcgtttc cgctcgagca tcgccgccag
      901 cacgtaacag gtattggccg gcgggttcgg cgacgcaatt aatcgccgaa aaagttgctc
      961 ggcggtttcc ttcgctaagg gctcgggtgg cggttccggc agcggttcgt agcgcgtctt
     1021 ccaaaacgaa aacggctgaa tattttcgtt ccggtttgtc cacgcgtctt cgctcaaatc
     1081 ttcgcggcga tagccgtcgg cgtcgtggaa gagcaaagta taaaagtact caccgggcgt
     1141 gaacggccgt tgggtcacag cgcatcggtc ggcacgatgt ttgatcgccc attcgttcgg
     1201 ccacgggttc atcgggcaac tatacgccgc ggctgaaaga gtcgaaagcc cagcgcttcg
     1261 cggttcgagg cccggaggta gaacaggtaa aggccgatga cggtgaaaag aacattcggc
     1321 gtccatgccg cgagcaatgg agagatgcgg tcgccttctc cgagcgctag aaacaggtgg
     1381 gtgagaaaat tcatcgagaa cacaaggatg accgcgctgg cgacactcgc cagaacgccg
     1441 cgacgtgaat acccgatgcc gagcggcgcc gcgatgcaga cgacgacaaa acaagtccac
     1501 ggcagcgcga gacgatactg aaaatgagtg cggaacggcg cgagcaaggt cgcgggaaaa
     1561 tccgcgttga agtgcagata atcgcgcagc tccggcaggc tgagatattc cgcgcgcgca
     1621 ttcgcgctgc cgagccggaa aggcgtttcg ctccagtgct ggacaatgac ggaaggataa
     1681 aactgctcgt cgatcacgtt tccggacgcg tcgtagcgga ccaccttcgc gttgctcagc
     1741 tcccagctct gcgactcggc gtggtagacg gcgcggctgg cgagatagtt cgttacgata
     1801 tcgtcgttcg agtcctgctg cagcacctgg acattgttga attcgttgct gccgttgcgg
     1861 aaattctgga tgaaccaggt ccgcgcatcg gtgcgattgc gaaaaatctg gccttcaacc
     1921 agtgcctcgg gccggccacg cgcttcggag aaaaaattct tccgcacgag ttcggcgtgc
     1981 ggcgcgagcg aatagttcag cgccatggtc gcaccgacgg tgagcaaccc gatgatgaca
     2041 agcggcagga gaatgcgcgg cagactgatg ccggcggtca gcatcgagac gatctcgttc
     2101 gcgcgcgaca ttcgcccgag tgaaaagagc agcgcgagca gcagcgacac cggcagcagg
     2161 atgatgaaaa cctgcggcac ctgcgtgagg taatattgca ccgccagtcc taggccgacc
     2221 cgttcgtcga gcagtgtcga aatgttgtcg ctgatatcga agatgagcca gatagagata
     2281 aacccggcga tacaataaaa gtacgcctga acaaaattgc gaatgacgta gcggtcgagc
     2341 aggcgcacga gttaaggtaa gcaaatcgcg atgatcgcgc tgaaagattc tctcgggacc
     2401 gagtttgtcg agcgggtgcg cgcgatcttt tcggaaaatg ggctgctgtc gaaggcgagg
     2461 aacttcgagt ttcggctgca acagcaggaa atggccgcag cggtagcgca cgcgctggag
     2521 gaagatcgcc atcttgttgt cgaagccggc actggcgtcg gcaaatcact cgcctatctc
     2581 gtcccggcga ttctcttcgc gctcgaacag cacaaaaaag cgatcgtctc cacgcacacg
     2641 atcaatctcc aggagcaact cgttcataaa gacatcccga tcctgaagaa ggtgctgccg
     2701 gttgagttcg aagcggcgct gatgaaaggc cggcaaaatt atatctgcgg ccgcagactc
     2761 gaacgcgcgc tgcaaagcgc gaatgagctt ttcaccgggc cggagcagac cgaattacag
     2821 cggcttgcgc aatgggccgc gaccacacgc gacggttcgc tcagcgattt gtctatcgaa
     2881 cctgacccga aagtttggat gcaggtttgc agcgaagcgc acatctgcac gcagaagacc
     2941 tgtggccaaa acccacgctg tttttatcag caggcgcgca aacgcttgct cgccgccgat
     3001 gttatcgtcc tcaatcacac gctgctgttc atgctgctcg gcagtccgga aatgcaggag
     3061 gagcgcgaga gcggttttct cttcccgaac gatttcctta ttttcgatga ggcgcacaca
     3121 gtcgagcagg tggcatctcg ccagatcggg atcggcgtct cgcaatacgg gctaagatcg
     3181 acgattcaac ggctctacaa cgcgcgcacg cgaaaggggc tgttcaccgt gacgcgcgac
     3241 gcggccggtg tgcgactcgc cgcggaactg gtggacgacg tgaacaagtt ctttgcctcg
     3301 gtcgactcca aatccgactt ccgtaaaggc cgcgagttcc gcgttcgcga tgtcggtctt
     3361 gtgccggaca cgatcactgg ccgtctaacg gcattgcagg cgcgcatcat tgacgtcgcg
     3421 aagcgtgcgg acgacgaact gttgaaagcc gagctccaag agttcggccg ccgcatccgc
     3481 gatgcgcgcg acggtatcgc catttttctc gagcaaagcg cgcgcgagca cgtctattgg
     3541 gtcgagcgca ccggcaaaac cgagcgctac ctttcgctta acgccgcacc tgtcgatctt
     3601 gcgccggtgc tgcgccggat gctgtttcgc gagaacgctt gctgcatcat gacgagcgcg
     3661 accctcgccg tcggccggcc ggacctcgcg tacttccgcg aacggattgg cgccaccgac
     3721 gccgagccgt tgcagctcgg cagcccgttc gattttcaaa aacagacaaa actgttcgtg
     3781 gtgcaaaaaa tgcccgaccc gcgcgatgcg gattaccacc gggcgctcga gcgctggatt
     3841 gcgcattttg tcgagaagac cgacggccgc gcgtttgtcc tgttcaccag ctaccgcgac
     3901 ctgcaacagg tcgccggtga gatgcagaaa ttttttgttg ggaaaggaat gaacctgctc
     3961 gtccaaggcg gcggcgcgcc gcgcggcaaa ttgctc
//
LOCUS       DASTDG010000103         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1370214, whole genome shotgun sequence.
ACCESSION   DASTDG010000103 DASTDG010000000
VERSION     DASTDG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370214"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFO30_03245"
     CDS             complement(<1..486)
                     /locus_tag="VFO30_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418331.1"
                     /translation="MQDKQPANQNVSITVAWDDIVRFVRQLSHDLRNDLNAAELQAAY
                     LDEIGTDSEIKTEIRRLREMLARMGGHLQKLSARVAQPQPHMIEYRASEFLQDVRAKI
                     ERELGKDGAAVEWDIQPSDAVLNVDPQLFQEALLELFTNAFRHERDTAEPVIATGKIE
                     NG"
     gene            complement(498..2072)
                     /locus_tag="VFO30_03250"
     CDS             complement(498..2072)
                     /locus_tag="VFO30_03250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9418332.1"
                     /translation="MTTTSLWLRFQKYFLRYQHLGFSLDISRMNFPDDFFSRMQRKID
                     NAFAAMSELEAGAIANPDEQRMVGHYWLRNTKIAPSEELRREIEQTKERIKQFAAEVH
                     SGNITPERGGKFEHILLIGIGGSALGPQFVSDALGSERDPMDIYFLDNTDPDGFDRVF
                     RKTHNRFAQTLVVVISKSGGTKETRNDMLETEARFNKRGLSFNKHAMAVTGVGSDLDK
                     QAEKNSWLARFPMWDWVGGRTSVMSAVGLVPMALQGLKIDNFLRGAAAMDERTRVTQL
                     SRNAAMLLALMWFYAGNGRGEKDMVILPYKDRLLLFSRYLQQLVMESLGKEKDLDGNI
                     VHQGIAVYGNKASTDQHAYVQQLRDGVLNFFITFIEVRKDRHGERFEVEPGVTSGDYL
                     QGFLQGTRAALYESGRQSITVSMPDVDAFNVGALIALYKRAVGFYGSLVNINAYHQPG
                     VEAGKRAATKLLQLESKVRGQLSASPNTAEQIAQEIDADAEDVFHVLEHLASNNPDIV
                     ATAGEHPGADTFAIRN"
     gene            2207..>2748
                     /locus_tag="VFO30_03255"
     CDS             2207..>2748
                     /locus_tag="VFO30_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET9418333.1"
                     /translation="MQPVRGNGLPHLCAMLLASGVTLLILLAGRVLAVHREAKNIAAV
                     APELFPLKNQGLAFQRLAARTDGVLPLYGSSELLGPAGARASDFFRTAPTGFQVSPVG
                     KAGATTLITLEKLGAIGRQFRGRRVVISISSAYFASGVTSYWYEGNFSPFAVSELTFD
                     NEFDLALKRDIAARLLEFPHT"
BASE COUNT          547 a          822 c          747 g          632 t
ORIGIN      
        1 gccgttttcg attttgccgg tcgcaataac cggctcagct gtgtcacgct cgtggcgaaa
       61 agcgttggtg aaaagttcca gcaacgcctc ctgaaacagt tgtggatcga cattgagcac
      121 ggcgtcactg ggctggatgt cccactccac cgccgcgccg tctttgccga gctcgcgctc
      181 gattttcgcg cgcacgtctt ggaggaattc cgacgcgcga tactcaatca tgtgcggctg
      241 cggctgagcg acacgcgcgg acagcttttg taaatgtcct cccatgcgcg ccagcatctc
      301 gcgcagacgc cgaatctccg ttttgatctc ggaatccgtg ccaatctcat cgaggtaggc
      361 cgcctgcagt tcggccgcat tgagatcgtt gcgcaaatcg tgactgagtt ggcgaacgaa
      421 gcggacgatg tcgtcccacg ccacggtgat cgatacattt tgattcgcag gttgtttgtc
      481 ttgcatctga agatcggcta gttgcggatt gcgaacgtgt cggcacccgg gtgctcgccc
      541 gcagtggcga cgatgtcagg gttattgctc gccaaatgct caagcacatg aaatacatcc
      601 tcggcgtcgg cgtcgatctc ctgcgcaatt tgctctgccg tgtttggact cgccgaaagt
      661 tgtccgcgaa ctttgctttc gagttgcaac agcttcgtcg ccgctctttt tccggcttcg
      721 acgccaggct ggtgatacgc gttaatatta acgagcgagc catagaaccc aacggcgcgt
      781 ttgtagagcg caatcaaagc gccgacgtta aacgcgtcca cgtccggcat gctcacagtg
      841 attgattgcc gtccactttc gtaaagcgcc gcacgtgtcc cctgcaagaa cccttgcaaa
      901 taatcgccgc tcgtcacccc cggctcgacc tcaaaccgct caccatgtcg atctttccgc
      961 acttctataa aggtgatgaa gaagtttagc acgccgtcgc gcagctgctg cacataggca
     1021 tgctgatcag tcgaagcctt gttcccgtaa accgcaatgc cctgatgaac gatgttaccg
     1081 tccaagtcct tctccttgcc taacgactcc atcaccagtt gctgcaaata gcggctgaac
     1141 agcagcaagc gatccttgta aggcaggatc accatgtctt tttcgccgcg tccgtttccg
     1201 gcgtaaaacc acatcaaggc aagcaacatg gccgcgtttc gcgacaattg tgtgacgcgc
     1261 gtccgctcgt ccattgccgc cgccccgcga aggaaattgt cgatcttcaa cccctgcagc
     1321 gccatcggca caaggccaac cgcgctcatg accgacgtgc ggccgcccac ccagtcccac
     1381 atcggaaaac gggccaacca actgttcttc tccgcctgtt tgtccagatc gcttccgaca
     1441 ccggtgactg ccatcgcatg tttgttgaac gaaagaccgc gtttattgaa ccgcgcttct
     1501 gtctcgagca tgtcgtttcg ggtttccttt gtgccgccgg acttcgagat cacaacgacg
     1561 agcgtctgcg caaaacgatt gtgggtcttg cggaacacgc ggtcgaatcc atcgggatcg
     1621 gtgttgtcca gaaaatagat gtccatcggg tcacgttccg atccgagcgc gtcggacacg
     1681 aattgtggcc cgagcgcgga accgccgatc ccgattagca agatatgctc gaatttgccg
     1741 ccacgctccg gcgtgatgtt gccgctatgc acctccgccg cgaattgctt tatccgctcc
     1801 tttgtttgtt cgatctcgcg acggagctcc tcgctcggcg cgatcttcgt attccgcagc
     1861 caatagtgtc cgaccatccg ttgctcatcc ggattggcga tggccccagc ttccagctca
     1921 ctcatcgcgg caaacgcgtt gtcgattttg cgttgcattc gagaaaagaa atcatccggg
     1981 aaattcatcc ggctgatgtc caaggagaag ccgaggtgtt gataccgaag aaagtacttc
     2041 tggaaacgca gccagaggga agtcgtcgtc atgcgctcgc cgatgcgcgg cttcgaatat
     2101 taacggcacg ctgtcgcgaa tttgcaatcg ctgcggcggt gaaagagcaa aaaaccccgg
     2161 gctccgactt gcgaacggat cagccgcggg tataagcggt tcgcctatgc agccagtgcg
     2221 cggtaacggc ctgccgcatc tctgtgcaat gttgctggcg tctggagtga cgctgctcat
     2281 tttgctcgca ggccgggtat tggcggtgca tcgcgaggcg aagaacattg cggctgtcgc
     2341 gccggaactt tttcccctca aaaaccaagg cctggcgttt cagcgccttg ccgcacgcac
     2401 cgatggtgta ttaccactct atggtagttc ggagttgctc ggaccagctg gcgcgagagc
     2461 gagcgacttc tttcgcactg cgccgactgg cttccaagta tcgccggtgg gcaaagcggg
     2521 agcgactacg ctcattacct tagaaaagct gggcgcgatt ggccgccagt tccgtggaag
     2581 aagggtcgtc atctcgattt cttcggcata tttcgcatcc ggcgtcacct cttactggta
     2641 cgaagggaac ttctcgccct ttgcagtcag tgagctgacg ttcgataatg agttcgatct
     2701 tgcgctcaag cgcgacatcg ctgcacggct gttggagttt ccccacac
//
LOCUS       DASTDG010000104        13456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_253799, whole genome shotgun sequence.
ACCESSION   DASTDG010000104 DASTDG010000000
VERSION     DASTDG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253799"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2776
                     /gene="dnaE"
                     /locus_tag="VFO30_03260"
     CDS             <1..2776
                     /gene="dnaE"
                     /locus_tag="VFO30_03260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9418334.1"
                     /translation="DFPEAITNALEIAERCNLEIELGKSKYPEYPLPEGVMREQYLRE
                     LCANGLQWRYGDRAKIDNQLRERLEYELGVLEKTGFVSYILIVWDFIRFAKEKGIPVG
                     PGRGSAAGSLVAYVLGITDIDPLQFGLLFERFLNPDRVSPPDIDVDFCEARRGEVLEY
                     VRQKYGERRVAQIITFGKLKAKSVVRDVGRVMGLSYRDADRIARMIPNEINVTLESAA
                     EKNPELKRAIESEPETKQLFEYGKILEGLSRNAGVHAAGVVIADRDLTDYIPLCRDVK
                     GNDVISQYPMGPLNDLGLLKMDFLGLKTLTALEDSVTLIRKREPNFSLKDIPLDDAAA
                     FALCNRGETVGIFQMESGGMTNLAKQFDIQKLDDIIALIALYRPGPMELIPEYVRAKK
                     GITPIKYLHPLLEEICADTYGVMIYQEQVMAAASKLAGYSLAQADLLRRAMGKKDKPK
                     MAKERKNFIDGCARANKIAEKKANAIFDLLEKFAGYGFNKSHSAAYGIISYQTAYLKA
                     HYPVEFMAGLLSNEINNTEKISVLVAECKRLGVPILPPDINKSGLKFSPEKCGTGILP
                     VKPEGHRQDADATLAIRYGLAAIKNVGESAMQVAISERERAGEFKSMEDFCGRMGTRV
                     ANRKMLESLIKAGAFDFTGCDRAELFDCIDEALGLAAAAHRDRIAGQVSLFDDDAHSG
                     TRRKRTVTAWSEHEKLSYEKELLGFYVSGHPLDRYVDLFAAKNYQSINSLGELDDRAS
                     FSTAGAILHVERKFTRREGKPFAVVWIEDLSGSLEVVVWNDVYLKVSDALTSGRVVEI
                     RATIDTRGDSLRATAQNVKVLSPHANGLGNGNHRSTNTAGEPAVLLQFSSATTSEELR
                     QVREILATSPGQRRVQLILDRPSGGLVCVDAGADLRVELTRDLEQKLERWLVTSKAQW
                     RNPTADPATS"
     gene            complement(2751..4703)
                     /locus_tag="VFO30_03265"
     CDS             complement(2751..4703)
                     /locus_tag="VFO30_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418335.1"
                     /translation="MRARNRLLLIALLALALLLFSIASAPFTVANAVRVWLWWNARQQ
                     GLAVSTDYINAPLFHPVVVRGLRVTSSRNAVARLDANVAEATVGLNVRGFLLHRKMRA
                     IRAITLDGFHLSVHQGTESGQLVNQAGWRALHNLLPDAFNIQHGEVRVEDRDTVVLLR
                     NVSFSGSEIEAGTFQADQVMIASPWLRQTFGQLRGGTKWQENRLTIAGLSLTTGVDLA
                     SVTFDLSELGDRAIGIHFDAEVFGGEIRADISHDWRSAGAISNVAGSASEISLAQLAA
                     AIGFTDHIRGLLHACKFSLRGNLLDPLHATGWMWTELTHLQWHDRALDLAMFGAAFNH
                     RQVAVEQLYLKQSGNELTLTGEATLPPRLQDWMKLNLRADIAASIRNLANLALLCGAK
                     PTAFGGELSVAGSLKAHDRKFDGQLHVEGSGLTISNAAVDLLRARVNLSGTQLGLEQL
                     ELARGGDFLHAEGKIDISPEHAAQGSLVFSVANLTDYFPHGLPASGVSAQLSFQGHAA
                     AIDSLTLRSGSARVDFTGTTNFADLRKIGATITPEQPLFESGALATAECISGLQLLPL
                     GTGAQSPPQIQTIALQGSIFTGDWQLILKKDAGPDEFTRICRESMGRRLEVMVGSEKN
                     REFGANVLQAFRTGDRKPLSLSLDQQ"
     gene            complement(4711..5364)
                     /locus_tag="VFO30_03270"
     CDS             complement(4711..5364)
                     /locus_tag="VFO30_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET9418336.1"
                     /translation="MSWPLGLAVIALIAAVLIGFIFYRTETWPMRTTHQGLEESERVA
                     RDMKNAFVSMAHLQPRITINNRVYVEQTTPTSELALVSRQIEVEHEFVHTWIGSSKRV
                     KLHGTFNVKAGFDLRQNLAVDVRPNDIVIHLPHAQILDVTQQRADVLAFENGFWNRIS
                     AADVQNELSILPQLARQKAAEGQLPAEAERALQQQLEQRVHTAQPLKLVFAEPRPID"
     gene            5613..6161
                     /locus_tag="VFO30_03275"
     CDS             5613..6161
                     /locus_tag="VFO30_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9418337.1"
                     /translation="MRNAQGGKRQSLTGSLLIAHPNMLDPNFRRTVLFISVHDPEDGA
                     VGIIINRPLDKQLADVVTEPPPAGLADVPVFLGGPVGKNQLMIAALEWQKGEGLKLNQ
                     KVALEEPNDDGATVCAFVGYAGWTAGQLETELEQKAWLVRKPSRSLVRLERLPQLWFD
                     IMRGLGPWYKMLAAAPDDPSLN"
     gene            6244..7341
                     /gene="ald"
                     /locus_tag="VFO30_03280"
     CDS             6244..7341
                     /gene="ald"
                     /locus_tag="VFO30_03280"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9418338.1"
                     /translation="MIIGVPKEIKEQEQRVALLPSGAQQLVRRGHSVLAEKNAGIGSG
                     YADDEYVRAGAEIVDFAADVFRRAELIVKVKEPLKAEFPLLRKGQILFTYLHLAASRS
                     LTEALLASGVIAIAYETIQVGNRLPLLEPMSEIAGRMSVVMGANFLAKYNGGSGVLLG
                     GVPGVLPGRVVVLGGGTSGVNAARMAGGLGADVAILDVDLERLRYLDLAMGHTNTLFS
                     NEANLSDLMPECDLLIGAVLLPGAKAPKLITRAMLRQMKRGSVLVDISIDQGGCAETS
                     RPTTHIDPVYVEEGVTHYCVANMPAAYARTATQALTNVTYRYVELLADKGLKGACEKQ
                     PALIGGINTREGHLTCRAVADAHGLKYEPPF"
     gene            complement(7372..7923)
                     /locus_tag="VFO30_03285"
     CDS             complement(7372..7923)
                     /locus_tag="VFO30_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418339.1"
                     /translation="MSASYGEGEELVVLVVVSLLLSLEGEELVLMVVLDSVLLEVAGD
                     GFTTVVLLSFFSPPGGLVTVVSFCSHATSRAAPASMQMYFFIFEFDPWSYGEGEVAGD
                     SVVVVVVVVPVVASGLIAAVASGEVVVVGLPDGVTVSVFCSQAASRAALARMQMYFFI
                     VVVVRRIFGQLWIRSKPAFRPWI"
     gene            8029..8391
                     /gene="rnpA"
                     /locus_tag="VFO30_03290"
     CDS             8029..8391
                     /gene="rnpA"
                     /locus_tag="VFO30_03290"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9418340.1"
                     /translation="MPQRSLKFRKTQRLTRESEFRQVRQRGRIARGNLFTLGLLQNES
                     AAPVRAGIITSRKLGRAVVRNRVRRRLREIIRRHQYEVAAGTWMVIIARPSAARVSYQ
                     RLEEEWLRLAKRASILSA"
     gene            8391..8648
                     /gene="yidD"
                     /locus_tag="VFO30_03295"
     CDS             8391..8648
                     /gene="yidD"
                     /locus_tag="VFO30_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9418341.1"
                     /translation="MVRAIRLLIRIYEYTLSPLLDLLSGPGSGCRFQPTCSQYFLQAV
                     EGHGVFRGGWLGLQRIARCHPWGDCGHDPVPPRICVQFVSE"
     gene            8666..10387
                     /gene="yidC"
                     /locus_tag="VFO30_03300"
     CDS             8666..10387
                     /gene="yidC"
                     /locus_tag="VFO30_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9418342.1"
                     /translation="MDRTAWIVITLCVIGLILWEIYVAKQTRPNPAMVTAPSPAPTAP
                     SALASSTPAPVASPVAAASAIPATTIPSFQEKIDTLRSSDVELRLTNRGGGIAEAVLL
                     NHKAEGDQRVVLNSPQRTPIGALVDNPAAPLLPEFKMTRQGNAMQLDYTTPEQVLVRK
                     TLFFQTSAAHKDEFVAELDVDLTNQGTQPYEKPGYFVALGSAVPIHPKDYPSYTRLAW
                     CVDGRARGIDVGWFGGGGGLLGIGQRSAQSFYQQSVGGAEWVAVCNQFFTTLIAPLSA
                     KAINTWAQRFEIAQWPGQKAFGIEGALGMPGFQLPPGQSYRAKFELYLGPKIYHRLAQ
                     LEHNEAEIMDFGIFKLICQFLLNFMNLLHSWFKDYGLAILGLTTIIKLVLWPIQNRAN
                     RSMRRMAALSPKMQELREKYKDDPTRMNQEVMKLYKDYGINPVGGCLPMLIQIPIFFG
                     LFKMLGQAVELRNAKFLWVRDLSQPDTVAHLPVLGWPVNIIPLCMAATQIWLMAMTPK
                     TGDATQRRVMMFTPLIFLFICYNFAAALALYYTTQNLFSILQFYQNKRQPVPTLQKVA
                     PAAKRKR"
     gene            10384..10869
                     /locus_tag="VFO30_03305"
     CDS             10384..10869
                     /locus_tag="VFO30_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET9418343.1"
                     /translation="MMTPKELLDTMLGYLGFVVQIEESQNDAGNLVLQIYTEESGRLI
                     GREGETLEAITFLLNRLLQAKDKDAPKVVVDCEHYRSMRDDRIVQRVRELAERVRITG
                     RSLQLEPMNSYERRIVHNAFKDDPDVATWSPSDSARIKQITLLKRQPKREPAAQPAPQ
                     K"
     gene            complement(10944..12116)
                     /locus_tag="VFO30_03310"
     CDS             complement(10944..12116)
                     /locus_tag="VFO30_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9418344.1"
                     /translation="MKVIVVGAGINGVTAAIELKKRGHEVTLIDSGPLPHPLAASTDI
                     SKAVRASYGPDEDYTELVERAVPLWRKWNEAFGTKLYHETGFLFMRRREMKPGDFEFE
                     SFKILERRGHSVERIGPAKLRERFPAWSAELFPDGFFEATAGYAESGRVVSVLVERAT
                     SLGVRLRAAQFSELLESAGVVKGIALEDRQQILGDAVVMAVGAWTPFLLPFTQKFFRA
                     TGHPVFHLKPRNPESFLADRFPVFGADISMTGYYGFPIGREGVMKIGSHGAGREMSPE
                     SSQRAVTAEEEKRMREFLSWAFPVLADAPIVYSRICLYCDTHDGNFWIAPDPERGGLS
                     IATGDSGHGFKFAPVLGELIADAVEGRTNAWSHKFRWRPEVQPGIHKEAARFQTQL"
     gene            complement(12187..13176)
                     /locus_tag="VFO30_03315"
     CDS             complement(12187..13176)
                     /locus_tag="VFO30_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-enoyl thioester reductase domain-containing
                     protein"
                     /protein_id="HET9418345.1"
                     /translation="MRESIQTVVYERHGNPAEVLRVESQPWPRPAADEAAVEMRAAPI
                     NPADLNAIEGKYPARPQLPATPGFEGAGVVVDVGAEVTGLTTGARVILPHNLGTWREA
                     VAVKASELVAVPPEIEPVHAAMLKINPMTAWRLLHDYVDLARGDWLIQNAANSAAGRA
                     VIEIARELGLKTVNVVRRAELIEELRDEGGDIVLVDSENLRDEVKNATSGAPIRLGLN
                     AVGGGSALRLANCLAPSATLVTFGAMSLQPLKIPNGLLIFKDLRFRGIWINKWYDNAT
                     IAERMDAFRLLFDMARRGLLRTKVEKAYPIGEAKAAVAHAARSQRSGKIIFEF"
     gene            13179..>13456
                     /locus_tag="VFO30_03320"
     CDS             13179..>13456
                     /locus_tag="VFO30_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9418346.1"
                     /translation="MNSAEQTEAAEKNLQTHQATTLSVWETGARVALLGLVINTVLAS
                     AKIAAGILGNAYVLIADGIESALDIGGSLVIWGGLKLAARPPDATHPYG"
BASE COUNT         2942 a         3954 c         3836 g         2724 t
ORIGIN      
        1 agattttccg gaagcgatca caaacgcgct cgaaatcgcg gagcgctgta acctcgagat
       61 tgagctcggc aaatcgaagt atcccgagta tccgctgccg gaaggtgtca tgcgcgagca
      121 atacttgcgc gaattgtgcg cgaacggtct gcagtggcgt tacggcgacc gcgctaagat
      181 cgacaatcaa ctgcgcgagc gtctcgaata cgagctgggc gtgctcgaga aaactggctt
      241 cgtcagttac atcctcatcg tttgggattt catccggttc gcgaaagaga aggggattcc
      301 cgtcgggcca ggtcgcgggt cggctgccgg gtcgctggtc gcctatgtcc tcgggattac
      361 ggacatcgat ccgctgcagt tcggactgtt gttcgagcgc ttcctcaatc ccgatcgcgt
      421 ctcgccgccg gatatcgatg tcgatttttg tgaagcccgg cgcggcgaag tcctcgaata
      481 cgtacgacaa aaatacggcg agcggcgcgt ggcgcagatc atcacctttg ggaagttgaa
      541 ggcgaaaagc gtcgttcgcg atgtcggccg ggtgatgggg cttagctatc gcgacgctga
      601 ccgcatcgcg cggatgatcc ccaacgagat caacgtcacg ctcgagtcgg ccgcggaaaa
      661 gaaccctgaa ctcaaacgcg cgatcgaatc ggagccggag acgaagcagc ttttcgagta
      721 cggaaaaatt ctcgagggcc tttcccgcaa cgccggcgtg cacgccgccg gggtcgtaat
      781 cgccgatcgc gatttgactg attacattcc actctgccgc gacgtgaaag gcaacgacgt
      841 catcagccag tacccaatgg gaccgctgaa cgaccttggc ctccttaaga tggatttcct
      901 cggattgaag acgctgaccg cgctcgagga cagcgtgacg ctgattcgca aacgcgagcc
      961 gaacttctca ctcaaagaca ttccgctgga cgacgcggcg gcattcgcac tgtgcaaccg
     1021 cggcgagacg gtcggcattt ttcaaatgga gtctggcgga atgaccaacc tggcgaagca
     1081 attcgacatt caaaagctcg acgacatcat cgcattgatc gcgctctatc gccccgggcc
     1141 gatggaattg attccagagt acgtcagggc aaaaaagggg atcacgccga tcaaatatct
     1201 gcatccgtta ctggaagaga tctgcgccga cacttacggc gtgatgattt accaggaaca
     1261 ggtgatggca gcggcgagca aattggccgg ctattcactt gcgcaagccg acctgttgcg
     1321 ccgcgcgatg gggaaaaagg acaagccgaa gatggcgaag gagcgcaaga atttcatcga
     1381 tggttgcgcg cgcgccaaca aaatcgcgga gaagaaggcg aacgcgatct tcgatttgct
     1441 cgagaaattc gccggttacg gattcaacaa gagtcacagc gcggcctacg ggattatcag
     1501 ttatcagacc gcatatttga aggcgcacta cccggtcgag ttcatggccg gcctgctcag
     1561 taacgaaatc aacaacacgg aaaaaatctc cgtcctcgtc gcggaatgta agcgcctcgg
     1621 cgtcccgatt ctgccgccgg acattaacaa aagcggcctc aaattcagtc cggaaaaatg
     1681 tggcacgggc attctgcccg tgaaaccaga aggtcatcgg caagatgccg atgccacgct
     1741 ggcaatccgt tatggacttg ccgcgatcaa gaacgttggt gagtccgcga tgcaggtggc
     1801 catctcggag cgcgagcggg caggggaatt caagtcaatg gaagattttt gcggccgaat
     1861 ggggacacgc gttgccaacc gtaaaatgtt ggaaagcctg atcaaagccg gtgcgtttga
     1921 tttcaccggc tgcgaccgcg cggagctatt cgactgcatt gacgaagcac tcggcctcgc
     1981 cgccgcggcc caccgcgatc ggatcgccgg ccaggtatct ctcttcgatg acgacgcgca
     2041 ctcgggaacg cggcgcaaac gaaccgttac ggcgtggagc gaacatgaaa aactgtcgta
     2101 tgaaaaagag ctgctcggtt tttacgtgag cggtcatccg ttggatcgat acgtcgatct
     2161 tttcgcggcc aaaaattacc agtcgatcaa ttcgttaggc gagctggacg accgcgcttc
     2221 cttcagcacg gccggggcga ttctgcacgt cgagagaaag ttcacgcgca gggaaggcaa
     2281 accgttcgcc gttgtgtgga ttgaggacct ctccggctcg ctcgaggttg ttgtttggaa
     2341 cgacgtttat ctgaaggtct ccgatgcgct cacatcgggg cgcgtcgtcg aaattcgagc
     2401 caccatcgac acgcggggcg attcgctgcg cgcgaccgcc caaaacgtga aggtattgtc
     2461 gccacatgca aacggtctcg gaaacggcaa tcacaggtcg acaaacacgg ccggcgaacc
     2521 ggcggtgctg ttgcaattct cgagtgcaac aacgagtgag gagctccgtc aggtgcgcga
     2581 gattctggcg acttcacccg gacaacgtcg ggtgcagtta atcttggatc ggccgagtgg
     2641 aggtttggtc tgtgtcgatg ccggtgccga cttgcgtgtt gagcttaccc gcgatctcga
     2701 acaaaaactc gagcggtggc tggtcacgag caaagcccag tggcgaaatc ctactgctga
     2761 tccagcgaca agctgagcgg ttttcggtcg ccggtacgaa aagcctgcaa gacgttcgcg
     2821 ccaaactcgc ggtttttctc gctgccaacc atcacctcta gtcttcgtcc cattgactcg
     2881 cggcagatcc gggtgaattc atcgggcccg gcatctttct tcaagatcag ctgccaatcg
     2941 cctgtaaaga tgctgccctg caaagcgata gtctgaatct gcggcgggga ttgtgcacct
     3001 gtcccgagcg gcagcaactg caagccgctg atgcattcgg ccgttgccag tgcgccactt
     3061 tcgaacagcg gctgctcggg cgtaatcgtg gcgccgattt ttcgtagatc ggcaaaattc
     3121 gttgttccgg tgaaatccac gcgcgctgag ccggagcgaa gcgtcaacga atcgatcgca
     3181 gctgcatggc cttgaaaact caattgcgcc gacacaccgg acgcaggtag gccatgggga
     3241 aagtaatcag tcagattggc gaccgagaac acgagtgaac cttgcgccgc gtgttccggc
     3301 gatatgtcga tcttgccttc tgcatgcagg aaatcgccgc cgcgggcaag ctccagctgt
     3361 tccaggccaa gctgagtccc gctcaaattg acgcgcgcgc gcagcagatc aacggccgcg
     3421 ttcgagatcg tcagtccaga gccctcgacg tgtagttgcc cgtcaaattt tcgatcgtgc
     3481 gccttgaggg atccggctac tgagagctct ccaccgaacg cggtgggctt tgcaccgcat
     3541 aacagagcca ggttcgcaag attgcgaata gacgccgcga tgtctgcgcg aaggttcagc
     3601 ttcatccagt cttgcagcct cggtggcagc gtggcctcgc cggtaagcgt cagttcgttg
     3661 ccgctctgct tcagataaag ctgctcaacg gcgacttgtc gatggttgaa ggctgcgccg
     3721 aacatggcca gatccagcgc acgatcgtgc cactgcaagt gagtcagctc ggtccacatc
     3781 cagccggtgg cgtgcagcgg atcgagcaag ttgccgcgca aactgaattt gcatgcgtgc
     3841 agcaacccgc ggatgtggtc cgtgaacccg atggcggcgg ctaattgcgc gagcgaaatt
     3901 tcactcgccg aaccggcgac gttcgagatt gcaccagccg agcgccagtc atgcgaaata
     3961 tccgcgcgaa tttctccacc gaaaacttcc gcatcgaaat gaatgccgat agcacgatcg
     4021 ccaagctcgg ataaatcgaa ggtgacggag gcgagatcga ccccggttgt aagactcagg
     4081 cccgcgatcg tcagacgatt ctcttgccat ttcgtgccgc cgcggagttg accaaaagtc
     4141 tgccgcaacc atggcgaagc aatcatcacc tggtcagctt ggaaagtgcc ggcctcgatt
     4201 tcactgcccg aaaacgacac attccgaagc aacacgacgg tatctcgatc ttccactcgc
     4261 acttcgccgt gctgaatgtt gaaagcgtcc ggcaacagat tgtgcaaagc gcgccagccg
     4321 gcttggttca ccaattgccc ggattcggtt ccttggtgga cgctgagatg aaaaccgtcc
     4381 agcgtgattg cgcggatagc gcgcattttg cgatgcaaga gaaaccctct gacattcagg
     4441 cccaccgtcg cctccgcgac atttgcatcg aggcgggcta cggcgttgcg cgaacttgtc
     4501 acccgcaatc cccggacgac aaccgggtgg aaaaggggtg cgttgatata gtcggtgctc
     4561 actgcaagcc cctgctgccg cgcgttccac cacaaccaga cccgtacggc gttggcgacc
     4621 gtgaatggtg ccgaggcgat gctaaagagc agcagagcca gcgcgagcag cgcgatcagc
     4681 agaagccgat ttcgagcgcg cattgccagg tcaatcgatt ggccgcggct cagcaaaaac
     4741 caatttcaac ggctgcgccg tgtggacacg ctgctcgagt tgctgttgga gtgcgcgctc
     4801 ggcttcagcg ggcaactggc cttccgccgc tttctgccgc gccaattgcg gcaagatcga
     4861 caattcattt tgcacatcgg cggccgagat gcggttccaa aaaccatttt cgaacgcgag
     4921 cacgtccgcg cgctgctgtg taacatcgag aatttgcgcg tgtggcaggt gaatgacgat
     4981 gtcgtttggt cgaacgtcga cagccagatt ctggcgcaaa tcgaagcccg ctttgacatt
     5041 gaaagtgccg tgaagtttta cacgcttgga gctgccaatc cacgtatgaa caaactcgtg
     5101 ctcgacctcg atttgtcgtg agaccagcgc cagctcactg gtcggcgtag tctgctccac
     5161 gtagacgcgg ttgttgatgg tgatgcgcgg ttgcagatgc gccatgctga caaaggcgtt
     5221 cttcatgtcg cgcgcgacac gttcgctctc ctcgaggcct tgatgcgttg tgcgcatcgg
     5281 ccatgtctcg gtgcgataaa agatgaagcc gatcaacacc gccgcgatca gcgcaataac
     5341 ggccaagccg agcggccatg acattccact cgtcgagttt ggaggtggcg agcgttttct
     5401 tttcacgcca aaaaagtacc gccccccacg cccgaggggc gtgaagggcg gctgttcccc
     5461 ccagaacact cttggaaaac tggcttcgta gtaaccgagc ttaccggccg tggcaacaaa
     5521 attctcatgg ttcagctcgg cgtcaccggc cggtattttt gggcggtgcg ccagccagtt
     5581 gcgctaagcc atcgcgcgcg ttctaatttt gtatgcgaaa tgcgcagggt ggaaaacgtc
     5641 agagtctcac tggctcgctc ctgatcgcgc atccgaacat gctcgacccg aattttcgcc
     5701 ggacggtact gttcatctcg gtgcatgatc cggaggatgg cgcggtcgga ataatcatca
     5761 accggccact cgataagcag ctggcggacg tggtgactga gccgccgccg gctggcttgg
     5821 ccgatgtgcc ggtgtttctc ggcgggccgg tgggcaaaaa ccagctaatg atcgcggcgc
     5881 ttgaatggca aaaaggcgaa ggactcaagc tcaatcaaaa agtcgcgctc gaagaaccga
     5941 acgatgacgg cgctacggtt tgcgcttttg ttggctacgc tggctggact gcggggcaac
     6001 tcgagacgga gctcgaacag aaggcctggc tggtgcgtaa gccaagccgc tcgctagtaa
     6061 gactcgagcg tttgccacag ctttggttcg acattatgcg cgggctcggg ccatggtaca
     6121 aaatgctcgc cgccgcgccg gacgatccgt ccttgaattg aagcgattgg tttacgcgag
     6181 gtgagaagta attggtcttg attccaactt ctcactcatc actaatcact tcgcactcgg
     6241 ctcatgataa tcggcgttcc gaaggagatt aaggaacagg aacaacgtgt cgcgctccta
     6301 ccttccggcg cgcaacaact ggtccggcgc ggccattccg tcctcgcgga aaaaaatgcc
     6361 gggattggtt ccggctacgc cgacgacgaa tacgtccgtg ctggcgcgga gattgtcgat
     6421 tttgcagcgg acgtttttag gcgcgccgag ctgatcgtga aggtgaagga gccattgaag
     6481 gcggaattcc cacttttgcg caaaggccag attctcttca cgtatttgca tctcgccgcc
     6541 agcagatcgc taaccgaagc gttgctcgcg tctggcgtta tcgccatcgc ctacgaaacg
     6601 attcaggttg gcaatcggct tccgctgctg gagccgatga gcgagatcgc cggccgcatg
     6661 tcggttgtta tgggtgccaa ttttctggcc aaatacaacg gcggcagtgg cgtgttgctc
     6721 ggcggcgttc cgggcgtttt gccgggtcgc gtggttgtgc tcggcggcgg cacctccggc
     6781 gtgaacgcgg cgcgcatggc cggcgggctt ggcgccgacg tcgccattct cgatgtcgat
     6841 cttgaacggc tgcgctacct agatctggca atgggtcaca cgaacacgct tttttcgaac
     6901 gaagcaaatt tgagcgacct gatgccagaa tgcgatctgc tcatcggcgc ggtgctgttg
     6961 ccaggcgcaa aagcgcccaa actcattacg cgcgcgatgt tgcgccagat gaagcgcggc
     7021 agcgtgctcg tcgacatttc gatcgaccag gggggttgcg cggagacctc gcgaccaacg
     7081 acccacatcg atccggtcta tgtcgaagaa ggcgtgaccc attattgcgt ggcaaatatg
     7141 ccggcggctt atgcgcgcac cgcgacccag gcgttaacga acgtgacgta tcgctatgtc
     7201 gaactgctcg cggataaagg cctcaaaggc gcatgcgaga aacagcccgc gttgattggt
     7261 gggatcaaca cgcgcgaagg ccacctgacg tgccgggcgg tggctgacgc acacggtttg
     7321 aagtacgagc caccattttg atcaaaccag agtagctgcg caaaaacctg ctcaaatcca
     7381 aggccgaaaa gcgggcttgc tgcgaatcca cagttgtccg aatatgcgtc tcacaaccac
     7441 cactatgaag aaatacatct gcatcctggc aagcgccgct ctgctcgcgg cttgtgaaca
     7501 gaaaaccgaa acggtgacgc catctggcag tcccaccaca accacctcac ccgaagcgac
     7561 tgcagcgatg agcccagagg cgacaaccgg cacgacaacc acaaccacga ccaccgaatc
     7621 tcccgctact tcgccttcgc cgtaactcca cgggtcaaat tcaaatatga aaaaatacat
     7681 ctgcatgctt gctggtgcag ctctgctcgt cgcgtgcgaa caaaaagaaa cgaccgtgac
     7741 caatccaccg ggcggtgaga aaaaagagag caacaccacg gtcgtgaatc cgtctccggc
     7801 aacttcgagc aagacggaat ccaacacgac catcaatacg agttcttcgc cttcgagcga
     7861 gagcaagagc gagacgacta ccagcacgac cagctcctcg ccctcgccgt agctcgcaga
     7921 caagtcatcc cgagaagtct tcctccgggc gcggctctgg gggaagattt ctgctgtaca
     7981 gccgtcgatg cttgacgctt tacgcgcttt gccagatcga caatcgcgat gccacagcgc
     8041 tcattgaagt ttcgcaagac acagcgtttg acgcgtgagt cggaattcag acaggtgcgc
     8101 caacgagggc ggatcgcgcg gggcaatctg tttacgctcg gcctgctgca gaatgaaagc
     8161 gccgcgccgg tgcgtgccgg catcatcact tcgcggaaac ttggtcgggc tgtcgtgcgc
     8221 aatcgcgtcc gccgtcgctt gcgcgagatt atacgccggc accaatatga agtcgctgcg
     8281 ggaacttgga tggtgatcat cgcgcggccg agcgccgcgc gtgtgagcta tcagcggttg
     8341 gaggaggaat ggttgcgcct cgcgaagcgc gcttctatcc taagtgcctg atggtccgcg
     8401 cgattcgtct cctcattcgc atctacgaat acacgctttc accgcttctc gatctgctga
     8461 gcggccccgg cagcggttgt cggtttcaac caacgtgctc gcagtacttt ttacaagcag
     8521 tcgaggggca cggcgtcttt cgcggcggtt ggctcggttt gcaacgcatc gcacgctgcc
     8581 atccttgggg tgattgcgga cacgatcctg taccgccgcg aatatgtgtt cagttcgtca
     8641 gtgaataagc gcagctgcag gagttatgga ccggaccgcg tggatcgtca ttacactttg
     8701 cgtaatcggc ctgatccttt gggaaatcta cgtcgccaaa cagacgcgcc cgaacccggc
     8761 gatggtgacg gctccgagtc cggcgccaac cgcgccctcg gcgctcgcga gtagcacgcc
     8821 tgcgccggtt gcttcacctg tagccgcagc gagtgcaatt ccagcgacca cgattccgtc
     8881 gtttcaggaa aagatcgaca cgcttcgcag ttccgatgtt gaattgcgcc taacgaatcg
     8941 cggcggaggc atagccgaag cagttctact gaaccataaa gcagaaggtg accagcgcgt
     9001 cgttctaaac tcgccacagc ggacgccaat cggcgcgctt gtcgataacc ccgctgcgcc
     9061 cttgctaccg gaatttaaaa tgacgcgcca aggcaacgcg atgcagctgg attacaccac
     9121 acccgagcag gtgttggttc gcaaaacgct ttttttccaa acatctgccg cgcacaaaga
     9181 cgagtttgtc gccgaattag atgtcgatct tacgaaccaa ggcacgcagc cttacgaaaa
     9241 gccaggctac tttgtggcgc ttggctcggc cgtgccgata cacccgaaag attatccctc
     9301 atacacacgg ctggcttggt gcgtcgacgg ccgcgcaagg ggtatcgacg tcggctggtt
     9361 cggtggtggc ggcggccttt taggaatcgg ccaaaggtca gcccagtcgt tctaccagca
     9421 aagtgtcggg ggcgcggaat gggtggcggt ctgcaatcaa tttttcacga cgctgatcgc
     9481 gccgctcagc gcaaaagcaa tcaacacttg ggcgcaacgc ttcgagatcg cccagtggcc
     9541 cggacagaaa gcgtttggca ttgaaggcgc gttgggaatg ccgggattcc agctgccacc
     9601 cggacaaagt tatcgcgcga aatttgagct ctatctcggg ccgaaaattt accaccgcct
     9661 cgcccagctc gagcataacg aagccgagat tatggatttc ggcatcttca aactgatctg
     9721 ccaattcctc ctcaatttca tgaacctgct gcacagctgg ttcaaagatt acggcctcgc
     9781 catcctcggg ctgacgacga tcatcaagct cgtgctctgg ccgatccaaa acagagcgaa
     9841 ccgctccatg cgccggatgg cggcgctctc cccgaaaatg caggagctcc gcgagaagta
     9901 caaggatgat ccgacgcgga tgaatcagga ggtgatgaag ctttacaagg attacggcat
     9961 caatcccgtg ggcggctgtt tgcccatgtt gattcaaatc ccgatcttct tcggtctgtt
    10021 caaaatgctc ggccaagccg tggaattgcg aaacgccaaa ttcctttggg tgcgcgatct
    10081 ttcccagccc gacacggtcg cgcatctgcc ggtactcggc tggccggtca acattattcc
    10141 actgtgcatg gccgcgacac agatttggtt gatggcgatg acgccgaaaa ccggggacgc
    10201 cacgcagcgt cgcgttatga tgttcacgcc gctgatcttt ttatttatct gctacaattt
    10261 tgccgctgct ctggcattgt attacacaac ccagaacctg tttagcatcc tgcaatttta
    10321 ccagaacaaa cgacaaccgg taccaactct gcaaaaagta gcgccagccg cgaaacgaaa
    10381 acgatgatga cgccgaaaga gttgctcgac acgatgctcg gctaccttgg gttcgtggtg
    10441 cagattgagg agtcgcaaaa cgatgccggc aatctcgttc tgcaaatcta tacggaggag
    10501 agcggacggc tcatcggccg cgagggcgaa actctcgagg cgatcacatt tctgctcaat
    10561 cgcctgctcc aggcgaaaga caaagatgca ccaaaagtcg ttgtcgattg cgagcactat
    10621 cgttccatgc gcgacgatcg gattgtgcag cgtgttcgcg agctggccga acgggtccgc
    10681 attaccggac gctccttgca gctcgagccg atgaattcct acgagcggcg gatcgtgcac
    10741 aacgccttca aagatgaccc cgacgtcgcg acttggagcc catcggactc ggcacggatc
    10801 aaacagatca cgctcctaaa acgtcagccg aagagagaac ccgctgcgca gccggcaccg
    10861 caaaagtgat tgtcgatgtt tctgtgtaga ggcgcttgtc ctcacgtgtg aacctaaggt
    10921 cctgcgccta acgaaagccg ccgctacagc tgcgtttgaa accgcgcggc ctctttgtga
    10981 attcccggct gcacttccgg tcgccagcga aatttgtgcg accacgcatt tgttctgcct
    11041 tccaccgcgt cggcgatgag ctcgccgagt acaggggcga acttgaatcc gtgtccactg
    11101 tcgccggttg cgatactcaa gccgccacgc tcggggtccg gcgcgatcca gaaattccca
    11161 tcatgcgtat cgcagtagag gcagatgcgc gagtaaacaa tcggcgcgtc cgctagcacc
    11221 gggaatgccc acgacaagaa ctcgcgcatt ctcttttctt cctcagccgt gactgcgcgc
    11281 tgcgacgact cgggcgacat ctcgcggccc gcaccgtgac ttccaatctt catcacaccc
    11341 tcgcgtccga ttggaaaccc atagtagccg gtcatcgaaa tgtcggcgcc aaacacggga
    11401 aaccggtcgg ccaaaaatga ttcgggattg cgcggcttga gatgaaaaac cggatggccg
    11461 gttgcgcgaa agaatttctg cgtgaacggc agcagaaacg gtgtccacgc gccaacggcc
    11521 atgaccactg cgtcacccaa aatctgttgt cgatcttcca atgcaatgcc tttgactacg
    11581 ccagcacttt ccaggagctc ggaaaactgc gcggcgcgca atctgacccc gagcgacgtc
    11641 gcgcgctcaa ccagcacact cacgacacga ccactttccg cgtatcccgc cgttgcctca
    11701 aaaaaaccgt ccggaaaaag ttccgcgctc cacgctggaa aacgctcgcg caattttgct
    11761 gggccgattc gttccacaga atgtccgcgg cgctcgagta ttttaaagct ctcaaactcg
    11821 aagtcgcctg gcttcatttc gcgtcgacgc atgaaaagaa agccggtctc gtgatagagc
    11881 ttcgtcccga acgcctcgtt ccattttcgc cacaacggaa cggctcgttc gaccagctcg
    11941 gtgtagtcct catccggtcc ataactggca cgcacggcct tgctgatgtc ggtcgaagcc
    12001 gcgaggggat gcggcaacgg accggaatca atcagcgtca cctcgtggcc gcgtttcttc
    12061 agctcaatcg cagcggtaac gccgttgatg ccggccccaa cgacgatgac tttcacgagc
    12121 caaattgtaa cgtaagcgtc tcgcttgcga accaatgaag aaagcaagct ggaagcttgc
    12181 cctactctaa aattcgaaaa tgattttgcc gctgcgttgc gatcgcgccg catgcgcgac
    12241 ggcggctttc gcttcaccaa tcgggtaagc tttctccact ttggttcgca acagtccgcg
    12301 ccgcgccatg tcgaaaagca gccgaaatgc atccatccgc tccgctatcg tcgcattgtc
    12361 gtaccatttg ttgatccaga ttccgcggaa gcgcagatct ttgaaaatca gcaaaccgtt
    12421 tggaatcttc agcggttgca aactcatcgc gccgaatgtg accagcgtcg cgcttggtgc
    12481 gaggcagttc gcgagacgta acgcactccc accgccgacg gcatttaatc caagccggat
    12541 cggcgcgccg ctcgtcgcat tcttcacctc atcgcgcagg ttttcgctgt cgacgagcac
    12601 gatgtcgcca ccttcgtcgc gcaattcctc gatcaactcc gcgcgcctga cgacgttcac
    12661 ggttttcaaa ccgagctcgc gcgcgatttc gatgactgcg cggccggccg ccgagttagc
    12721 ggcgttttgg attaaccaat cgccgcgcgc aagatcgaca taatcgtgca gcaaacgcca
    12781 tgcggtcatc ggattaattt ttaacatcgc cgcgtgcacc ggttcgattt ccggcggcac
    12841 cgcaaccagt tcgctcgctt tcaccgccac ggcttcgcgc cacgtcccaa ggttgtgcgg
    12901 cagaatcacg cgcgcgcctg tcgttaggcc ggttacttct gcaccaacat cgacaaccac
    12961 accagccccc tcgaatcccg gcgtcgccgg cagctgggga cgcgccggat atttaccttc
    13021 gatcgcgttc aagtccgcag gattaatcgg cgccgcgcgc atttccacgg cagcttcatc
    13081 ggcggcaggt ctcggccacg gctgactttc cacgcgcaat acctcagccg gatttccgtg
    13141 cctttcgtaa actacggtct gtatactctc cctcatcgat gaactcagcc gagcaaactg
    13201 aggctgctga gaagaatctg caaacacacc aggctacgac cttgtccgta tgggaaactg
    13261 gtgcgcgcgt cgctttgctc ggtctggtga tcaatacggt gctcgcatcg gcaaaaatcg
    13321 cggccggaat tctcggcaac gcctacgtcc tgatcgcgga cgggattgaa tcggcactgg
    13381 atatcggcgg ctcgctcgtg atttggggcg gactcaagct ggcagcgcgt ccgccggacg
    13441 caacgcaccc ttacgg
//
LOCUS       DASTDG010000105         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_438116, whole genome shotgun sequence.
ACCESSION   DASTDG010000105 DASTDG010000000
VERSION     DASTDG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438116"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFO30_03325"
     CDS             <1..372
                     /locus_tag="VFO30_03325"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9418347.1"
                     /translation="LATASPSVGGQEEEEEIPKCRKCGALMRPGVVWFSEMLDPRKID
                     MVESYVGHAPCELVLVIGTTALFAYIVDWAIRAARDGGELIEINPAETQLSNAATQTI
                     REPAAVALPKLVDALTCRGRC"
     gene            461..1861
                     /locus_tag="VFO30_03330"
     CDS             461..1861
                     /locus_tag="VFO30_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9418348.1"
                     /translation="MNRPDRSRIALSREEMRSFGYRVVDLLAEHFAGMERGPVGAKVD
                     PARIQKLLAAFPPEDGRQPRELLAQLEREVFPNNLHVDHPRFFAFVPGPNNFVSTMAD
                     ALAAGFNIFNGTWLGGSAAAALERGVVGWFCRMCGLPETAGGLFVSGGSMANLTALVA
                     ARHALLQDRVERAIVYFSDQTHASVERALRVIGFSPEQMRKLPSDDKFRLSVAGLRTT
                     IAEDRGKGLRPFCVIANAGTTNTGAVDPLPELADLAAAEKMWLHVDGAFGAASVLSPR
                     GKNLLAGLERADSISLDPHKWFFQSFECGCVLVRDMAQLKSAFQVRPDYLRDVHREAA
                     EFNPCDYGVQLTRSFRALKVWMSFETFGAAAFRQAITRGFELAELAERELRARRGWQI
                     VSPAQMATVCFRFGETDTVQTKLVDAMLRDGYAFLTSTTLKGVTCLRLCTINPRTTEA
                     DVIGTVKRLDELARQL"
     gene            complement(1955..2623)
                     /locus_tag="VFO30_03335"
     CDS             complement(1955..2623)
                     /locus_tag="VFO30_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418349.1"
                     /translation="MADHRNRVEDEAPQLLGVGTEPEFAIGQRALLLQSPDGNLLWDC
                     ITLVDDQTAAELNARGGVRAIAISHPHYYSAMVEWADRFDAQILLHAQDRQWVTRKSP
                     RIQFWEETTLSLWDGLTLINCGGHFEGGTVLHWPGGGNREGALLSGDIIQVVQDRRYV
                     SFMRSYPNLIPLGPTAIQRILAAIEPFAFEQIYGAWWKANVLSNAKDAVIRSAERYLR
                     AIND"
     gene            complement(2953..3603)
                     /locus_tag="VFO30_03340"
     CDS             complement(2953..3603)
                     /locus_tag="VFO30_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HET9418350.1"
                     /translation="MKQFFLSTAAAAAFALASCDWEGIGGNGHIVNDHRTVEDFAELH
                     TSGGAFDIEWRSGAPALTITTDENLLPYIETRKIDNRLELRTRRRIHSRRGIKILVSS
                     STRSGAKLSGASDLKVPALTGDKFALQSSGAAEVKLDGAVDKLLVDMTGASALKAKSL
                     QARSVGISTTGAGSAQVTATESLRVAITGAGDVTYFGNPKTVEKHVTGAGSIHHKD"
BASE COUNT          704 a         1070 c         1149 g          821 t
ORIGIN      
        1 ctcgccaccg cgagtccgtc cgttggcgga caggaggagg aggaggagat tccaaagtgc
       61 cgcaagtgcg gcgcactcat gcgacccggc gtggtctggt ttagcgagat gctcgacccg
      121 cgcaagatcg acatggtgga aagttatgtc ggtcacgcgc cgtgcgaact tgtcctggtg
      181 atcgggacga ccgcgctttt tgcctacatt gttgattggg caattcgcgc cgcgcgtgac
      241 ggtggcgagc tcatcgagat caatccggct gaaacgcaat tgtccaacgc ggcgacgcaa
      301 acaattcgcg aaccagccgc cgtcgcgttg ccgaaattgg tcgacgcctt gacttgtagg
      361 ggacgctgtt agcgtcccgt aattgctttc tcctacagaa tttttcgcgc agcattagcg
      421 acggctccca agatcggcta cgagtcagtc gagcacaggt atgaatcgac ctgatcggtc
      481 ccggattgcg ctttcgcgcg aagagatgcg cagcttcggc tatcgcgtgg tcgatctgct
      541 ggccgaacat ttcgccggga tggagcgcgg gccggtgggg gcgaaagtcg atccggcccg
      601 gatccaaaaa ctgttggctg catttccgcc ggaggacggc cggcagccgc gcgaactgct
      661 tgcccaactc gagcgcgaag ttttcccgaa caatctgcat gtcgatcacc cgcgtttctt
      721 cgccttcgtg cccgggccta acaatttcgt cagcaccatg gccgatgcgc tcgcggccgg
      781 gttcaatatt tttaacggca cgtggctcgg cggatcggcg gcggccgcgc tcgaacgcgg
      841 agtagtcggt tggttttgcc ggatgtgcgg cctgccggaa acggcgggcg gtcttttcgt
      901 cagcggcggc tcgatggcca atctcaccgc gctcgtcgcc gcgcgtcacg cactgctgca
      961 agatcgggtc gagcgcgcga tcgtctattt ctcggatcaa acgcacgctt cagtcgagcg
     1021 cgcgctgcgc gtcatcggct tctcgcccga gcagatgcgg aaacttcctt cggacgacaa
     1081 gttcagatta tcagtcgccg gcttgcgcac caccattgcg gaagatcgcg gaaaaggttt
     1141 acggccgttt tgtgtaatcg cgaatgcggg cacgacgaac actggcgcag tggatccatt
     1201 gccagagctg gcggatctcg ccgcggccga aaaaatgtgg ctgcacgtcg atggcgcatt
     1261 cggcgcggct tcggtcttat cgccgcgtgg gaagaatctg ttggccggtt tggagcgcgc
     1321 cgattcgatc tcgctcgatc cgcacaaatg gttcttccaa tccttcgagt gcggctgtgt
     1381 tttggttcgt gacatggcgc agttgaaatc ggcctttcaa gtccggccgg attatctgcg
     1441 cgacgtccac cgcgaagccg cggagtttaa tccatgcgat tatggagttc agctgacgcg
     1501 gagttttcgc gcgctgaaag tctggatgtc gttcgagacg ttcggcgccg ccgcattccg
     1561 acaagcgatc acgcgcggat tcgagcttgc cgagctggcc gagcgcgagc tgcgcgcgcg
     1621 tcggggttgg caaatcgtct cgccggcgca aatggccact gtgtgttttc ggttcggcga
     1681 gactgacacg gtacaaacga aattagtcga tgcgatgttg cgcgacggct acgcgtttct
     1741 gacctcaacc acgctcaaag gcgtcacctg cctgcgcctt tgcacgataa acccgcgcac
     1801 aaccgaagct gatgtcatcg ggacggttaa gcgcctcgat gaactcgcgc ggcaattgta
     1861 ggggcccagc tagtatctgt gacagcgaca cgtacattcc ggcggaggcg agcgcgaacc
     1921 acatgaggat tcggatcgca aacggttcct tcacttagtc gttgatcgcg cggagatagc
     1981 gttcggccga gcgtatgacg gcatctttcg catttgaaag gacgttggct ttccaccagg
     2041 cgccgtaaat ttgttcgaat gcaaacggtt cgattgcagc gaggatgcgc tgaatcgccg
     2101 tgggcccaag cggaatcaga ttcggatagc tgcgcatgaa actgacgtag cggcggtcct
     2161 gcactacttg aatgatatcg ccgctgagca gtgccccttc gcggtttccg ccgcccggcc
     2221 aatgcagcac ggtgccacct tcgaaatggc cgccgcagtt gatcagggtg agcccatccc
     2281 atagcgacag cgttgtttcc tcccaaaatt gaatacgggg acttttgcgc gtcacccatt
     2341 gtcgatcttg ggcgtgcaaa agtatttgcg cgtcgaaccg atcggcccat tccaccatcg
     2401 cggaataata atgcgggtgc gaaatcgcga tcgcgcgaac gccgccgcgc gcgttcagtt
     2461 cggcggccgt ttggtcgtcc accaatgtga tgcagtccca gaggagattt ccgtcgggcg
     2521 actgcaataa caatgcgcgc tggccgatgg caaactccgg ttcggttccg acaccgagaa
     2581 gctgcggcgc ttcgtcttcg acacggttgc gatggtccgc cactaatcgt tctagcgtgg
     2641 tccactcctg cccgccgtgc ctgacgaatt gcctttcgtc ttcacaaatc ggacagcata
     2701 gcgggggcgc cgttgtctcc gcaaactgcg tgccgcattg gacgcagata aaattttcca
     2761 tcgcgcgctg agcctaacac atcgcggcag cacactgtag gggacgctgt tagcgtccca
     2821 tttcgggaag caaactgccc attcgacctt cgctcagggc aagactttcc cctacagttt
     2881 tatgctaacg caggtcaatg cgccgctgcg ttaaggatcg cgatccaaaa aataagcagg
     2941 actgacatag ttttagtcct tgtggtgaat ggatccggcg ccggtgacgt gtttctcgac
     3001 ggttttcggg ttaccgaagt aagtgacgtc gcctgcgccg gtaatggcga cgcgtagcga
     3061 ttcggtggcg gtgacctgcg cgctgcccgc gccagtcgtg gaaattccaa cactgcgcgc
     3121 ctgcagcgac ttcgccttta aggcgctcgc gccagtcatg tcgacgagca gtttgtcgac
     3181 tgcgccatcg agcttcactt cggcggcgcc gctggattgg agagcaaatt tgtcgccggt
     3241 cagcgccggc actttcagat cgctcgcgcc gctgagtttt gcgcctgaac gggtggaact
     3301 ggagacgagt attttgatgc cccgtcgcga gtgaatccgt cggcgggtgc gcagttcaag
     3361 tcgattgtcg atcttccgcg tctcgatgta cgggaggaga ttttcgtcgg tcgtgatggt
     3421 gagcgcaggt gctccgctgc gccattcgat gtcgaaggcc ccgccactgg tgtggagttc
     3481 ggcgaaatcc tccactgtgc gatgatcgtt gacgatgtgg ccattcccgc caattccctc
     3541 ccaatcacaa ctggcgaggg cgaatgcggc ggctgcagcc gtagagagga agaactgttt
     3601 catgacgggt gctttaggtg attcgatgcg gcggcagtct gttttggatt cgggttttta
     3661 atgtggcgcg agttcccaac tcgcgaatcc atagagcgca acttggaagg ttgcgcgaca
     3721 ttcacctcgt gatcctcagt ttga
//
LOCUS       DASTDG010000106         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1588215, whole genome shotgun sequence.
ACCESSION   DASTDG010000106 DASTDG010000000
VERSION     DASTDG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588215"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="pilM"
                     /locus_tag="VFO30_03345"
     CDS             complement(<1..1152)
                     /gene="pilM"
                     /locus_tag="VFO30_03345"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9418351.1"
                     /translation="MPTSRQILSLNIGSQTIGLAKFRMQPHGGLVLIDYRLRELLPDA
                     SGDSHAKLPGVLREMMAELHLRGGTVNYAAAGQSVFARFVKLPAVEEEKIERIIAFEA
                     QQNVPFPIDEVVWDYQLVGGGADEQIQVVLVAIKADLLDEINRAVEETGLRTAIVDVA
                     PMALYNAFRYNYSELSGCSLLVDIGARTTNLLFVEPGKIFSRGVPIGGTTITAAIAKD
                     FAESFAASEERKKRNVFVSLGGAYAEPEDVDLARASKIVRSTMTRLHAEIMRSISHYR
                     AQQQGSRPERVFLCGGSTSTRYMREFFQEKLQLPVEFFNALRNVAVSESATADATARS
                     AYLLGELVGLALRAVTTCPMELNLRPASVLRRQQLEKRRPFLAAAAACVI"
     gene            complement(1163..1744)
                     /locus_tag="VFO30_03350"
     CDS             complement(1163..1744)
                     /locus_tag="VFO30_03350"
                     /inference="COORDINATES: protein motif:HMM:NF042424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1102 family pilus-like protein"
                     /protein_id="HET9418352.1"
                     /translation="MRLQFRFGLLSVGLFLPGLLVGQTRSAREFQLTRITRNFISSPQ
                     FTYAGAEAFPVDTRQRWLQVEAEFTAAPPVTDELTLKYFVLFNATVFTAEVTHTNVLA
                     GRERRSVIYMPPRTVARFTPANRPLGPNSVQNIAVQIVRQGTVKDELSLNRAAVQWYA
                     SMPQVSGLLLNKSETPFAPLYWDRYEQIKSSGR"
     gene            complement(1777..2739)
                     /locus_tag="VFO30_03355"
     CDS             complement(1777..2739)
                     /locus_tag="VFO30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET9418353.1"
                     /translation="MPARLQPVSVVTGGAGFLGSHLTDRLLAEGHRVIAVDNLITGNT
                     ANIAHLAGNENYKFIKHNVSNFIFVPEEKIDYVFHFASPASPIDYLELPIPTLKVGAL
                     GTHNALGLAKDKRASFILASTSECYGDPLEHPQREDYWGNVNPIGPRGVYDEAKRFAE
                     AMTMAYHRYHRIDTKIVRIFNTYGPRMRLRDGRVVPAFIGQALSGKPLTVFGDGSQTR
                     SFCYVSDLIDGIYKLAMSDFHEPVNLGNPREMTIKQFANEIIRITGTTSTIECKPLPI
                     DDPKVRQPDITRARKVLGWEPRIEFEEGIRKTVEYFRQSLKSSA"
     gene            2994..>3752
                     /gene="trpS"
                     /locus_tag="VFO30_03360"
     CDS             2994..>3752
                     /gene="trpS"
                     /locus_tag="VFO30_03360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9418354.1"
                     /translation="MTARKLTVRILSGIQPSGVLHIGNYFGMMRPAIDLQREGETFYF
                     IADYHALTSVRDPKALRENSRRVALDFLACGLDPDRSVLFRQSDVPQVTELAWILSTV
                     APMGLLERAHSYKDKLARGLAPTVGLFSYPVLMAADILIYDSDAVPVGKDQKQHIEMT
                     RDLAVKINETYGEVFKLPEPRINPQTQVVPGLDGQKMSKSYGNNIDIFGDENETRKRV
                     MSIVTDSTPVEAPKDPARSTIFQLYSLFASKEELA"
BASE COUNT          801 a         1105 c         1053 g          793 t
ORIGIN      
        1 aatcacgcac gccgccgccg ccgctaagaa cgggcggcgc ttttccaatt gctgtctgcg
       61 aagaacgctc gctggccgca aattcagttc catcgggcag gtagtcactg cgcgcagcgc
      121 cagcccgacc agttcgccta acaaataggc ggaacgcgca gttgcatcag ctgtcgccga
      181 ttcgctcacc gcgacgttgc gcagtgcatt gaagaattcg accggcaatt gcaatttctc
      241 ctgaaaaaac tcgcgcatgt agcgcgtgct cgtgctgccg ccgcagagaa agacgcgctc
      301 gggccggctc ccctgctgct gcgcccggta atggctgatc gagcgcatga tctcagcgtg
      361 cagccgagtc atcgtgctgc gcacgatctt tgacgcccgc gcaagatcga catcctccgg
      421 ctccgcatac gcgccgccca gactgacaaa cacgttacgc ttcttccgct cctcggacgc
      481 ggcaaaggac tccgcgaagt ccttggcgat ggcggcggtg atcgttgttc cgccaatcgg
      541 cacgccccgc gaaaagattt tacccggctc gacgaataac aggttagtcg tgcgcgcgcc
      601 gatgtcgacg agcagcgaac aaccgcttag ctcgctgtaa ttgtagcgaa acgcattgta
      661 gagcgccatc ggcgcaacat cgacgatcgc cgtgcgcaga cctgtttcct ccaccgcgcg
      721 attgatctcg tccagcaaat cggccttgat cgccaccagg accacctgga tctgttcgtc
      781 cgcgccgccg ccgactaact ggtaatccca aaccacttcg tcgatcggga acggcacgtt
      841 ctgctgcgcc tcgaaagcaa tgattcgctc aatcttctcc tcctcgaccg ccggcaattt
      901 gacgaagcgc gcgaagaccg attggccggc ggccgcgtaa ttgaccgtgc cgccgcggag
      961 gtgcagttcg gccatcattt cgcgcaaaac accgggcagc ttggcatggc tgtctccaga
     1021 ggcatcaggc aaaagttcgc ggaggcggta atcgatcagc acgagcccgc cgtgcggttg
     1081 catgcggaat ttcgccaacc caatggtttg cgacccgatg ttgaggctga ggatttgtct
     1141 gctggtcggc atcgggaaaa cgtcagcgcc cggagctttt gatctgctcg taccggtccc
     1201 agtagagcgg cgcaaagggc gtttcgcttt tgttcaacag aagaccggaa acctgtggca
     1261 tgctcgcata ccattgcacc gcagcgcggt tgaggctgag ctcatccttg accgtgccct
     1321 gtcgcacgat ttgcacggcg atgttttgca ccgaattcgg gccgagcggc cgattggccg
     1381 gggtaaatct cgccacggta cgcggcggca tgtagatgac cgatcgtcgc tcgcgaccgg
     1441 cgagcacatt tgtgtgcgtg acttcagcgg taaacactgt ggcgttgaat aagacgaaat
     1501 acttcagcgt tagctcgtcc gtcaccggcg gtgccgctgt aaactctgct tccacttgca
     1561 gccaccgctg ccgtgtatca accggaaaag cttcggcgcc ggcatacgtg aactgcggcg
     1621 atgagatgaa gttgcgggtg atgcgggtga gctgaaattc cctcgccgag cgagtctgtc
     1681 cgacgagaag accgggcaaa aacaaaccca cgctgagcaa gccgaagcga aactgcagcc
     1741 tcatgagtag atgtcgatct tgcaaccctt ctgtgctcac gcgctgcttt tcaaggactg
     1801 gcggaaatac tcgaccgttt tccgaattcc ctcctcgaat tcaatccgcg gctcccaacc
     1861 gagcactttt cttgctcgcg tgatatcggg ctgccgcacc ttcggatcgt cgattggcaa
     1921 cggcttacat tcaattgtcg atgttgtccc ggtgatccga atgatttcgt tggcgaattg
     1981 tttgatcgtc atttcgcgcg ggttgcccaa gttcaccggc tcatggaaat cgctcatcgc
     2041 aagcttgtat atcccatcga tcaaatccga aacataacaa aacgagcggg tctgtgagcc
     2101 gtcgccaaaa accgtgagcg gtttgccgct aagggcctgg ccgatgaagg ccggcacgac
     2161 gcggccatcg cgcaaccgca tgcggggacc gtaggtgttg aaaatgcgga cgattttcgt
     2221 atcgatccga tgataccggt gataagccat tgtcatcgct tcggcgaacc gtttcgcctc
     2281 gtcgtaaacg ccgcgcggcc cgatcgggtt gacgttgccc cagtaatctt ctctctgtgg
     2341 atgctcgagc ggatcaccat agcattcgga ggtagacgcg agaatgaagg aggcgcgttt
     2401 gtctttggcc aatccgagcg cgttatgtgt gccgagcgcg cccactttca aggtcgggat
     2461 cggcaactca aggtagtcga tcgggctggc tggcgaggcg aagtgaaaca cgtagtcgat
     2521 cttctcctcc gggacgaaaa tgaagttgga aacgttatgc ttgatgaact tgtagttctc
     2581 attcccggcg agatgcgcga tgttcgccgt gttaccggtg atgagattgt cgaccgcgat
     2641 cacgcgatga ccttccgcca gcagccggtc ggtcaaatgc gagcctaaaa aacccgcgcc
     2701 gccagtcacg actgaaaccg gttgcaagcg ggcgggcatt tcgagtttat ctagcggcag
     2761 caggggaatt tagcgagcag aaatggtcaa ttgcagattg cagacttgaa cttgcagatc
     2821 tggtgtgacg attcgccttc cgtctgaaat ttgaaatctt caatttggaa attatccatg
     2881 tggattatcc ggcatccaac ccaccagcgt gctgctcatc ggttgtcatt tcgagccaag
     2941 tcgagaaatc tcttgctgtt caaacaaagt gagatccttc gacttcgctc aggatgacag
     3001 ctagaaaact aaccgtgcgc attctttccg gcattcagcc cagcggcgtt ctgcacatcg
     3061 gcaattattt cggcatgatg cggccggcga tcgacctgca gcgcgagggc gagaccttct
     3121 actttatcgc cgactaccac gcgctcacca gcgtccgcga cccaaaggcg ctgcgcgaga
     3181 attcccgacg cgtcgccctc gattttctcg catgcggtct ggatccagac cgttccgtgc
     3241 tgtttcgcca gtcggatgtg ccccaggtga cggagctcgc gtggatttta tcaaccgtcg
     3301 cgccgatggg gctgctcgag cgcgcccatt cctacaaaga caaactcgcc cgcggtttgg
     3361 cgccgaccgt cggactcttc agttatccgg tgctgatggc cgccgacatt ttgatttacg
     3421 acagcgacgc ggtgccagtc ggcaaggatc agaaacaaca catcgagatg acgcgcgacc
     3481 tcgccgtgaa gattaatgag acttacggcg aggttttcaa gttacccgag ccgcgcatca
     3541 acccacaaac ccaagttgtt cccggtctcg atggtcagaa gatgagcaag agctacggca
     3601 acaacatcga catctttggc gacgaaaatg aaacgcgaaa acgtgtcatg agcatcgtca
     3661 ccgattcgac gccggtggaa gcaccgaagg acccggcaag atcgacaatc ttccagctct
     3721 attcgctctt cgcgtcaaag gaggaactcg cc
//
LOCUS       DASTDG010000107         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1276769, whole genome shotgun sequence.
ACCESSION   DASTDG010000107 DASTDG010000000
VERSION     DASTDG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276769"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFO30_03365"
     CDS             <1..373
                     /locus_tag="VFO30_03365"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9418355.1"
                     /translation="DDQIKIIGERGGVIGVNAILVSPKPEESTIDRYVDHIEHIVDLI
                     GIDGVGLGFDFCEYLFQQLPASVHEELAAKLTTPHFISNLTNHTQTHALTRKLIERGF
                     TDEAIEKILFQNWMRILRQLL"
     gene            complement(383..736)
                     /locus_tag="VFO30_03370"
     CDS             complement(383..736)
                     /locus_tag="VFO30_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418356.1"
                     /translation="MPNARQDGNHESHLEQDICIHIFTVSSAMVGVCLTVIGLIRVVI
                     TLGKADTLADDFLALDALLFLTACLLAYWALRARSVCRRHRIERIADGIFIVAMIGMV
                     AICALITYSISLPPR"
     gene            complement(846..>2192)
                     /locus_tag="VFO30_03375"
     CDS             complement(846..>2192)
                     /locus_tag="VFO30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HET9418357.1"
                     /translation="SPGRTDRQLTVDELADRRDREVPEWLRLFYRRESLLNAWSQRAR
                     ENARRARDDLFGDANNRKRYDGYIAALLDPDIWRSIQAREQKLRDTISHDPKLKSAIA
                     AYDRIKKAQTQIVKVAPRYDYLEQERPITVGYRSPRAFYGTLFKYARLLTRAIDERSK
                     PNGERIPEFRDSAKESLELELFSTEPVYDDYEILRLTDSMTDFAEKFGADDPLVKQVL
                     AGKSPHARAIELVKGTKLKDVDVRKNLYAKDAAGLQAAHDPMLDLARLIDGPARELRK
                     LHDAQEEIKKQAYADIAKARFAIEGASNYPDATFTLRLSYGVVRGYEEDGKQIPPFTN
                     LAGLYQRASEHDNKPPFDLPQRWIDKKSALNLNTPFDFVSTADIIGGNSGSPVVNKKN
                     EFVGIIFDGNIQSLALDCIYTDKQARAVSVDSAAITEALRKVYEANTLADELEGGR"
BASE COUNT          455 a          636 c          616 g          485 t
ORIGIN      
        1 cgacgaccaa atcaaaatca ttggagaacg cggcggcgtc attggcgtaa acgccatcct
       61 cgtcagtcca aaaccggaag agagcacgat cgatcgttac gtcgatcaca tcgaacacat
      121 tgtcgatctt atcggaatcg atggtgtcgg cctcggcttc gacttttgcg agtacctctt
      181 tcagcagtta ccggcgagcg tgcacgagga gcttgcggcc aaattgacca cgccgcattt
      241 tattagcaac ctaacgaatc acacccagac gcacgctctc acacgcaagc ttatcgaacg
      301 cggcttcacc gacgaagcaa tcgagaaaat actgttccaa aattggatgc ggatcctgcg
      361 gcagctcctc tgacttggcg cctcagcgcg gcggcagaga aatgctgtag gtaatgagcg
      421 cgcaaatcgc caccatgccg atcatcgcca cgatgaatat cccgtcggcg attcgctcaa
      481 tgcggtgcct ccggcagacg ctacgagcgc gcagcgccca gtaggcaaga aggcacgcgg
      541 tcagaaataa caatgcatcc agcgccagaa aatcatcggc gagcgtgtct gccttaccta
      601 atgtaatgac gacgcgaatc agtccgatta cggtcagaca gacaccaacc atcgcagacg
      661 aaacggtaaa aatgtgaatg cagatgtcct gctcgagatg actctcgtga ttaccatctt
      721 gccgcgcgtt cggcatgggg tattagaccc ggtcctcaac ggcttcgttg gataaaactt
      781 agacgtcaat gagcggcaac acgcacgcaa ggcgcaaatc tccaagaacc gcctagcgct
      841 cagtgctacc tgccgccttc cagttcgtcc gccagcgtgt tcgcctcgta aactttgcgc
      901 agcgcctcgg tgatggcggc ggaatcaacg ctcacggcgc gggcttgttt gtccgtgtaa
      961 atgcagtcga gcgcgagcga ttgaatgttc ccgtcgaaaa tgatgccgac gaattcattt
     1021 ttcttgttca cgaccggact gccgctgttg ccgccgatga tgtcggcggt ggacacgaag
     1081 tcgaaaggtg tgtttagatt gagcgccgat tttttgtcga tccagcgctg tggaagatcg
     1141 aatggcggct tgttgtcgtg ctcggaggcg cgctgataca ggccggccag atttgtgaag
     1201 ggcgggattt gtttgccatc ttcttcgtag ccgcgaacaa ctccatatga cagccgcaag
     1261 gtgaaggtcg cgtccgggta attgctcgcg ccttcgatgg cgaagcgcgc cttcgcgata
     1321 tccgcatacg cttgcttttt aatttcctcc tgcgcgtcgt gcaatttgcg gagctcgcgc
     1381 gcggggccgt cgattagccg cgcgagatcg agcatcggat cgtgcgcagc ttgcaatcca
     1441 gccgcatctt ttgcgtacag gttctttcga acatcgacat ctttcaattt tgtgcctttt
     1501 accagctcga tcgcccgcgc gtgcggcgat ttgccggcga gcacctgttt cacaagcgga
     1561 tcgtccgcgc cgaatttctc ggcgaaatcg gtcatcgaat ccgtcagccg cagaatttcg
     1621 taatcgtcgt aaaccggttc ggtcgaaaac agctcaagct cgagcgactc cttcgcgctg
     1681 tcgcgaaact cgggaatccg ttcgccgttt ggtttactgc gctcgtcgat cgcgcgtgtg
     1741 agcagccgcg cgtatttgaa caatgtgcca taaaacgcgc ggggcgagcg gtaaccgacc
     1801 gtgatcggcc gctcctgctc gaggtaatca tagcgcggtg ccaccttcac gatctgcgtc
     1861 tgcgccttct tgatccggtc gtaggcagcg atcgccgatt tcaacttcgg gtcgtgtgaa
     1921 atggtgtcgc gcaatttttg ttcgcgcgcc tggatcgacc tccagatgtc cgggtccaac
     1981 agcgcagcga tgtagccgtc gtaacgtttg cgattgttcg catcgccgaa aagatcgtcc
     2041 cgcgcacggc gggcgttttc gcgcgcgcgc tggctccacg cgttcagcaa actctcgcgg
     2101 cggtaaaata ggcgcagcca ttccggcact tcccggtcgc gtcggtcagc cagttcatcg
     2161 acagtgagct gccggtcggt gcgccctggg ct
//
LOCUS       DASTDG010000108         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1200902, whole genome shotgun sequence.
ACCESSION   DASTDG010000108 DASTDG010000000
VERSION     DASTDG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200902"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFO30_03380"
     CDS             <1..527
                     /locus_tag="VFO30_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET9418358.1"
                     /translation="DTAGLRSSAEGVERAGIARTEKSLQTADLVLHVADRNAPKPPDF
                     ETRNENGNELALLNKSDLREHSDWHTTTALRISCLTGEGLAALEDEILARITKQNLRP
                     ESAVAINRRHRDCLRRALEACERAALALSNETPPEYVVVDLKEGLNAVGEVIGAVGVE
                     QILDSVFSQFCIGK"
     gene            524..1534
                     /locus_tag="VFO30_03385"
     CDS             524..1534
                     /locus_tag="VFO30_03385"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET9418359.1"
                     /translation="MSNGYERFVRPILFSLDPETTHDLTLAGLRAASHIDLALGALKR
                     FAPARNPKTVFGLTFPNPIGLAAGFDKNGVALPALAALGFGFIEIGTVTAQGQSGNSK
                     PRIFRLSEELALINRLGFNNDGADAIARRLAKLRAKRCWPAVPIGINIGKSRDTPLAQ
                     AAQDYLYCFHKLHPFADYIALNISSPNTPGLRSLQDRQLLAPLLRAFQQANAHARKPI
                     LIKIAPDLSEPQLVELIAVCAENEIAGIIATNTTIDHSSIPPTADEEGGLSGAPLREK
                     STALVRAIRARSTIPVIGCGGITDAASASEKFTAGAQLVQVYTGYVYRGPGLLREIVH
                     TL"
     gene            1647..1817
                     /locus_tag="VFO30_03390"
     CDS             1647..1817
                     /locus_tag="VFO30_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET9418360.1"
                     /translation="MLRWAFIFLIIALVAALFGFTGIASAAAGIAKFLFFLFLVICLV
                     FFILGISVAKKM"
     gene            complement(1824..2237)
                     /locus_tag="VFO30_03395"
     CDS             complement(1824..2237)
                     /locus_tag="VFO30_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418361.1"
                     /translation="MNRALKWKLLGGFVLVFIAGGTTGVFLGAAHARHSFFALHQNIM
                     AERMRHRLRAELQLSDEQMAKIAPIIDKTAARLQEIRHTTGQQVHETILEAHREIAPN
                     LTEDQRAKLQALEARPHHPRWFNHGRRPSPPGPGP"
     gene            complement(2234..2563)
                     /locus_tag="VFO30_03400"
     CDS             complement(2234..2563)
                     /locus_tag="VFO30_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418362.1"
                     /translation="MNRRSQSSGAEGGGSLDRLLRAAASTNEDAPEMPYGFDTRVVAL
                     WQAHGEREPNGIARLIRHVAVFAAVILIASAAGVYREVIRSDVSSEPFANQFAIADSA
                     IADEVSP"
     gene            complement(2560..3141)
                     /locus_tag="VFO30_03405"
     CDS             complement(2560..3141)
                     /locus_tag="VFO30_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9418363.1"
                     /translation="MEQPEARALVAAALRHDDDAARELVRRLHPLVAKLVRAHRPRRT
                     SEEDLAQMIFIKVFQKLWQFSGEVPLEHWVSRIAVNTCLNQIAAEKARPELRRSDLSE
                     EEEAVVENLAVSSDELAPDKRLAARQLVEHLLDLLKPAERLVMDLLYLQRRSVAEVQK
                     ITGWSASRIKVRAFRARQKMKKQLAKISAKENP"
     gene            3425..3817
                     /locus_tag="VFO30_03410"
     CDS             3425..3817
                     /locus_tag="VFO30_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9418364.1"
                     /translation="MKRNLLLLTAVVVLVLGGLAIAFAEAGPGGGPTGRAGWHSHGFG
                     LEHVTKALNLTPEQQAKVQPIVDQTRPQIVAIHRDAMEKAHAVVDQAMSQIRPLLTSE
                     QQKKLDDLKKAHDDMRKAHQELQEAMRE"
     gene            complement(4023..4226)
                     /locus_tag="VFO30_03415"
     CDS             complement(4023..4226)
                     /locus_tag="VFO30_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418365.1"
                     /translation="MSAEARKAVSLILICLAVLFFLLGMAARMKMNFRGRGEGSRIGL
                     TAAGVFAGAGIAMLAFSRSTRRE"
     gene            complement(4342..>4720)
                     /locus_tag="VFO30_03420"
     CDS             complement(4342..>4720)
                     /locus_tag="VFO30_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418366.1"
                     /translation="MLQPSFFSNKTEAGRRFAWQTRAEAAQQDYARAWIAALAPFGPT
                     QQSWICPSIQGFLGNPDLTKPQNLRVDYIATPFDDKPTTPHQSPRQPWFLESGDVHGH
                     GNLIIFTDGSVTDLKTVIKSSQP"
BASE COUNT          975 a         1366 c         1396 g          983 t
ORIGIN      
        1 tcgacacggc ggggttgcgc agctcggccg agggagtcga gcgcgccggc atcgcgcgca
       61 ccgagaaatc attgcaaacg gccgatttgg ttttgcatgt ggccgatcgc aacgcaccca
      121 agccgccgga tttcgaaacg cgcaatgaaa acggcaacga actcgctttg ctcaacaaga
      181 gcgacttgcg cgagcacagc gactggcaca caacgaccgc gctgcggatt tcctgcctga
      241 ccggcgaagg tctagccgcg ctagaggacg aaatcttagc gcgtatcacg aagcagaact
      301 tacggcccga aagcgccgtc gccatcaaca ggcggcatcg cgattgtctg cggcgcgccc
      361 tcgaagcttg cgagcgcgcg gcattggcgc tgagcaacga aacaccgccg gaatacgtgg
      421 ttgtggatct gaaagagggg ctgaatgcgg tcggcgaggt catcggggcg gtcggggttg
      481 aacagattct cgattccgtt ttcagccagt tttgcatcgg caaatgagca atggctacga
      541 acgattcgtc aggccaatcc ttttttcgct cgacccggaa acgacgcacg acctcaccct
      601 tgcgggcttg cgggcggcgt cacacatcga ccttgcgctc ggtgcgctga aaaggttcgc
      661 gccggcccga aatcccaaaa cagttttcgg tctaacgttc ccgaatccga tcggccttgc
      721 cgctgggttc gacaaaaatg gagtcgcgct gcccgccctc gccgcgctcg gcttcggctt
      781 tatcgagatc ggtaccgtta ctgcgcaagg gcagtccgga aactcgaagc cgcgcatttt
      841 tcgcttgtct gaagagctcg cgctgatcaa ccgccttgga ttcaacaacg acggcgccga
      901 tgcgattgcg cgccggctgg caaagttgcg cgcgaagaga tgttggcccg ccgtgccgat
      961 cggtatcaac atcggcaagt cgcgagacac gccgcttgcg caggcggcgc aagattatct
     1021 ttattgcttt cacaagttgc atccattcgc cgattacatc gcgctcaaca tcagctcgcc
     1081 gaacacgccg ggactgcgtt cgttgcaaga tcgacaattg ctggcgccgc tgctgcgcgc
     1141 gttccagcag gcgaacgcgc atgcgcgaaa gccaatcctg atcaaaatcg cgccggatct
     1201 atccgagccg cagcttgtgg aattgatcgc ggtttgcgcg gaaaacgaga tcgccgggat
     1261 catcgcgacg aacacgacga ttgatcactc gtcaattccg ccgacagcag atgaagaggg
     1321 cggcttgagt ggagcaccgt tgcgcgagaa atcgaccgcg ctggtgcgcg cgatccgcgc
     1381 aagatcgaca attcccgtca ttgggtgcgg cggcatcacg gacgctgcct ctgcgagcga
     1441 gaagttcacc gccggcgcgc agctggtgca ggtttacact ggctacgttt atcgggggcc
     1501 gggtttattg cgcgaaattg tgcacacgct gtaggcgttt gtgtcaaacg cttcgggttt
     1561 tacagcgttt cgcagaaacg ccctacaaat cttctgtgcc cgcaagcccg tgttgacgtg
     1621 cgatttaact ggcaggaggt gatcacatgt tacgctgggc ttttattttt ctgattatcg
     1681 cgctggtcgc ggcgttgttc gggtttacgg gcattgcttc ggcagcggcc ggaattgcga
     1741 agtttctctt ttttctcttc ctcgtaatct gcctcgtctt tttcatcctc ggcatttcgg
     1801 ttgcgaagaa gatgtagcct tgtctacggc ccggggccgg gcggcgaagg acgccgcccg
     1861 tgattaaacc agcgcgggtg atgtgggcgc gcttcgagtg cctgtaattt ggctcgttgg
     1921 tcttcggtaa ggttcggtgc gatttcgcgg tgcgcttcga ggatggtttc atgcacctgc
     1981 tggcccgtgg tgtgccgaat ctcctggagt cgcgcggcgg ttttgtcgat gatcggtgcg
     2041 atcttggcca tttgctcgtc gcttaactgg agctcggcgc gcaagcggtg ccgcatccgt
     2101 tcggccatga tgttttggtg caaggcgaaa aacgaatgac gcgcgtgcgc cgcgccgaga
     2161 aagacgccgg tcgttccgcc ggcgatgaaa accaggacga agccgccgag caatttccat
     2221 tttaacgcgc ggttcatggc gagacctcgt ccgcaatcgc cgaatcggca atggcgaact
     2281 gattagcgaa gggttcactg ctgacatcgc tgcggatgac ttcgcggtat acgccggcgg
     2341 cgctcgcgat caggattaca gctgcgaaca cggcaacgtg ccggatcagc cgcgcaattc
     2401 cgtttggttc gcgctcgccg tgtgcctgcc acagcgcgac cacgcgtgtg tcgaagccgt
     2461 acggcatctc cggcgcgtct tcgttcgtgg acgcagccgc tcgcaacaga cgatcaagac
     2521 tgccgcctcc ttctgcaccc gagctttgcg aacgccggtt catggatttt ccttcgcaga
     2581 aattttcgcg agctgctttt tcattttctg ccgggcgcga aacgcgcgca ctttaattcg
     2641 gctggcactc cagccggtta tcttttgcac ttcggccacg gagcgccgct gcaaataaag
     2701 caagtccatg acgagacgct cggcgggttt gagcagatcg agaaggtgct cgaccaattg
     2761 ccgcgccgcg agccgcttgt cgggcgcgag ctcgtcgctc gatacggcca gattttccac
     2821 aactgcctct tcttcttcgc ttagatcgga gcgtcgcagt tcgggacgcg ctttttcggc
     2881 ggcaatttga ttcaggcagg tgttaacggc gatgcgcgag acccaatgtt cgagcggcac
     2941 ttcaccggaa aattgccaca gcttctgaaa aactttgatg aaaatcattt gggcgaggtc
     3001 ctcttcactt gtccggcgcg gccgatgtgc gcgcacgagt ttagcgacga gcggatgaag
     3061 ccggcggacc agctcgcggg ccgcgtcgtc gtcgtgccgg agcgctgctg ccacgagcgc
     3121 gcgtgcttca ggttgctcca tgtcgttgct gccgcatcca cactaacaag tatgaccgcg
     3181 ccgcgcgcat cccggttacg gaattatcgc atccaagatc gacaaaatca gttgtagagg
     3241 cgcttgtgcc aagcgcctgt tttgaagctc ggcgcttgac acaagcgccg ctacaacacg
     3301 cgcgtcaccg attagccgtt agcatacgat aaactgtaac cactccgagg actggttggt
     3361 catcacctag caaagagcgc ggtaatgcgc gccgaaaaca aacagttaac aacgggaata
     3421 aacaatgaaa cgaaacttac ttctactcac agccgtcgtc gtcctcgtgc ttggcggttt
     3481 ggcaattgcc ttcgctgagg ctgggcccgg tggaggcccc accggtcgcg ctggctggca
     3541 cagccacggc ttcggactcg agcacgtgac caaggcgttg aacctgacgc cggagcaaca
     3601 ggcgaaggtc cagccgattg tcgatcaaac acgcccgcaa atcgtggcga ttcatcgcga
     3661 cgcgatggag aaggcacatg cggtggttga tcaggcgatg tcgcagattc gcccgctgct
     3721 cacgtctgaa cagcagaaga aactcgatga cctgaaaaaa gcgcatgacg acatgcgcaa
     3781 agcccatcag gaacttcaag aggcgatgcg cgaataagct cgctcgcctg ttgaattgat
     3841 gatgcggcgg cgggtcggca acgatccgcc gcctcttgtt gtagccaccg gcctgtggcc
     3901 agtcagacaa aaactggacg gctcatagag ccgtggctac agcaaactcg tgagcgcgcg
     3961 gttgtagtca ccacgctgcg ggcggtcatc cgaaacgcgc cagaggcgcg tggctacagc
     4021 atttactctc gtcgcgtgct gcgcgagaac gcgagcatcg caatacccgc gccagcgaaa
     4081 acgccggcag cggtgaggcc gatccgcgaa ccttcgccgc ggccgcgaaa gttcatcttc
     4141 atccgcgccg ccatgccgag cagaaaaaac aggacggcga gacaaattaa aatgagacta
     4201 acggctttac gcgcctcggc ggacattttc attccatgat acttgaacca cgaaggacac
     4261 gaagagcacg aagagatcac aagctcaaat gtttaatcct gcgttgcatt cagttcacct
     4321 aagtccttga cgcgatcagc atcacggctg cgacgattta attactgttt tgagatcggt
     4381 aacgctgccg tcggtaaaaa tgatcaggtt gccatgtccg tggacatctc cggactcgag
     4441 aaaccatggc tgccgaggcg actggtgcgg tgttgtcggc ttatcgtcga atggagtagc
     4501 gatataatcg acacgcaagt tctgcggctt cgtcagatca ggatttccga gaaaaccttg
     4561 gatactcgga caaatccagc tctgctgagt cggtccgaat ggtgcgagcg ccgcaatcca
     4621 cgcccgcgcg taatcctgct gagccgcttc cgctctcgtt tgccatgcga atctacggcc
     4681 agcttccgtt ttgttggaga aaaagctcgg ctgcaacata
//
LOCUS       DASTDG010000109         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1242946, whole genome shotgun sequence.
ACCESSION   DASTDG010000109 DASTDG010000000
VERSION     DASTDG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242946"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="VFO30_03425"
     CDS             complement(<1..2252)
                     /locus_tag="VFO30_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418367.1"
                     /translation="MNISEPFIRRPVGTSLLAAGVLLLGAVAYYFLPVAPLPKVDFPT
                     ISVSAQEPGVDPQTAASSLAAPLERRFAQIAGVSEITSVSSLGGSSITIQFDLNRDIN
                     GAARDVQSAINAASGELPSGLPQPPSYRKVNPSDAPIMVLAMTSDALPLSQVYNLADQ
                     IIGQRISQVDGVSQVTIGGGANSAVRVQINPVALASMGLSMEDIRTTLSQANVLSPKG
                     AFDGPEQSFVIASNDQLTQADQYQPIIVAHHNNAAVPLRDVGTAIDAQANRDQAGLFN
                     NKRAVLLVIFKQPDANVVRTTDQIHAILPQLRTWLPPSVHLDVMSDRTTTIRASVRDV
                     QLTLVITMALVVMVMFLFLRRFWPTFISGITMPLALAGTFGVMWLCSYSLDNLSLMAL
                     TVSTGFVVDDAIVVIENIVRFIEKGEPPLQAALKGARQIGFTVISISLSLVAVFIPLL
                     FMSGLIGRLFHEFAVTLSAAILVSGVVSLTLTPMLCGRFLKSEERHRQRGVIERLSER
                     GFNAMHDFYERGLKWVLKHEYTMLIVTGLVIIATVYLYFVVPKGFFPQQDTGQMMGTT
                     EAAQDISFEAMKEKQEQVVKTVMADPAVQAIGSFFGGGSGSALNNGRMFISLKPKGSG
                     KGERKDDAGTVIARLRQKLSKIPGAQLFLTANQDIRVGGRSSKAQYQYALSDQNIEEL
                     NSWAPKLVNKLHEYPQIKDATIDQQFRGLQETVVIDRDAAARLGIQPQAVDSTLYSAF
                     GQRQVSIIYT"
     gene            complement(2255..>4485)
                     /locus_tag="VFO30_03430"
     CDS             complement(2255..>4485)
                     /locus_tag="VFO30_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418368.1"
                     /translation="RTETIRASVRDVQFTLLLTVALVVMVMFLFLRKLWATVITSVAL
                     PLSLIGTFGIMYLAGFSLDNLSLMALTISTGFVVDDAIVMIENIVRYIEAGDGPLEAA
                     LKGAKQIGFTVVSLSISLVAVFIPLLFMTGIVGRLFREFAITMSVAVGVSAFVSLTLT
                     PMMCAKLLRSEKEEEREHQGEFYRRTEEYWTRFSNLYERGLRWVFAHQRFVLNIAIIT
                     LFATIFLYIVVPKGLLPQQDTGVIVGVTDAAENISFKAMLQRVHAVSDIVRRDPDVAS
                     VSASVGAGTVNATINTARLYIVLKPHNQRANAEKIIERLRNATRDVEGVSLFMQAAQD
                     LQIDARVSRTQFQYILQDADPAELAEWTPKLVQKLGQLPQLSDVASDQQVNGLQLNID
                     VDREKASRLNVLTQAIDDTLYDAFGQRQVSIIFTQLNQFRVILEVEPNFRESPDLLDK
                     IYVKSSSGQPVPLSAFATMHVSSTPLSIPHQGQFPAATISFNLRSGSSLSDAIPAIQK
                     TEREIGLPDTIETTFTGAAAEFRSSLTSEPLLILAAVVVIYIVLGVLYESYIHPITIL
                     STLPSAGVGALLALLICRIDFSLVALIGIILLIGIVKKNAIMMIDFALEAERKEGMSP
                     EESIYQACLLRFRPIMMTTAAALLGALPLALESGTGSELRRPLGISIVGGLLLSQFLT
                     LYTTPVIYLYLDRFGRWMKERRRRGGVAQAQLPLTTSTDLEHEPEPQFAGVNGEDNRL
                     GF"
BASE COUNT         1011 a         1252 c         1269 g          953 t
ORIGIN      
        1 tgcgtgtaaa tgatcgagac ctgccgctga ccgaatgcgc tgtaaagcgt gctgtcgact
       61 gcttgcggtt gaatgcccag gcgcgcggcc gcatcgcgat cgattaccac ggtttcctgc
      121 aatccgcgaa attgctgatc gatcgtggcg tccttgatct gtggatattc gtgaagcttg
      181 ttcacgagct tgggcgccca tgaattgagc tcctcaatgt tttgatcgga caacgcatat
      241 tggtattgcg cttttgacga tcttccgcca acacgaatat cttgatttgc tgtgagaaaa
      301 agctgcgcgc cgggaatttt cgagagcttt tgcctgagac gcgcgataac tgtgcccgcg
      361 tcatctttgc gttctccctt gcccgaacct ttcggtttca aactgatgaa catgcggccg
      421 ttattcaacg ctgagccgct cccgccgcca aagaatgagc cgatggcctg gaccgcggga
      481 tcggccatga cggttttcac gacctgctct tgcttttctt tcatcgcttc aaaggaaatg
      541 tcctgcgcgg cttcggtcgt gcccatcatt tgtcccgtgt cctgttgcgg gaaaaatccc
      601 ttcggcacaa cgaagtaaag gtaaacggtc gcgatgatca ccagcccggt gacaatgagc
      661 atggtgtatt cgtgtttgag gacccacttc aagccgcgtt cgtaaaaatc gtgcattgcg
      721 ttgaaaccgc gctcgctaag tcgttcgatc acgccgcgct gtcgatgtcg ctcctcgctt
      781 tttaaaaaac ggccgcagag catgggcgtg agcgttaacg aaacgacgcc cgaaacgaga
      841 atcgccgcgc tcaacgtcac cgcgaattca tgaaacaagc gcccgatcag gccgctcatg
      901 aacaacagcg gaatgaaaac cgcgacgagc gagagactaa tcgaaatgac ggtgaaacca
      961 atctgacgcg cgcctttcaa cgcggcttga agcggtggct cgcccttctc aatgaagcgc
     1021 acgatgtttt cgatgacgac aatcgcatca tcgacaacga atccggtcga aaccgtcaac
     1081 gccataagcg agaggttatc gaggctgtaa ctgcacagcc acatcacgcc gaaggtgccc
     1141 gcgagcgcga gcggcatcgt aataccggag atgaacgtcg gccagaaccg gcgcaggaag
     1201 agaaacatta ccatcacgac gagcgccatc gtaatgacga gcgtcagttg tacgtcccgc
     1261 actgaagcac ggatggtcgt cgtgcgatcg ctcattacat cgagatgcac gctcggcggc
     1321 agccaggtgc gcagttgcgg aaggatcgcg tgaatctggt cggtggtgcg aacgacgttc
     1381 gcgtccggct gcttgaaaat gactaataac acagcgcgct tgttgttgaa cagaccggct
     1441 tggtcgcgat ttgcctgtgc atcgatcgcc gtgccgacat cgcgtaaggg cacggccgcg
     1501 ttattgtggt gcgcgacgat gatcggctga tattggtcgg cctgcgttag ttggtcgttg
     1561 ctcgcgatga cgaaactctg ctccggaccg tcaaatgcac cctttggtga aaggacattc
     1621 gcttgtgaaa gcgtcgtgcg aatgtcttcc atgcttaggc ccatcgaggc gagcgcaacc
     1681 ggatttatct gcacgcgcac ggcggagttc gcgccgccgc cgatggtcac ctggctgacg
     1741 ccatccacct ggctgatgcg ctggccgata atttgatcgg cgagattgta aacttgtgac
     1801 aagggcaacg cgtccgacgt catcgcgagc accataatcg gcgcatcgga tggattgact
     1861 ttgcgatagg acggcggttg cggcagtccg ctgggcaatt ccccagacgc ggcattgatt
     1921 gcgctctgca cgtcgcgcgc cgcgccgttg atgtcgcgat tcagatcgaa ttggatcgtg
     1981 atggacgaac cgcctaacga actgacggaa gtgatctcag atacgccggc gatttgcgca
     2041 aagcggcgct cgagcggcgc ggcgagcgat gacgctgcgg tctgcggatc gactcccggt
     2101 tcctgcgcgc tgacagaaat ggtggggaaa tccactttcg gcaacggcgc caccggcaaa
     2161 aaataatagg ccacagcgcc cagcaacagc actcccgctg ccaacaggga tgtgccgact
     2221 ggtcgacgaa tgaatggctc ggaaatgttc atgcctagaa tccaagtcga ttatcttcac
     2281 cgttcactcc agcgaattgc ggctcgggtt cgtgttccag atctgtcgac gttgttaacg
     2341 gtagctgggc ttgagcaacc ccaccgcggc gacggcgttc cttcatccaa cgtccaaacc
     2401 gatcgagata aaggtaaatc accggcgtcg tgtacagcgt gagaaattgg gagagcaaca
     2461 atcctccgac gatggaaatt ccgagcgggc gccgcagttc actgccagtt ccactctcga
     2521 gggcaagtgg caatgcgccc aacaaggccg ccgccgttgt catcataatg ggccgaaacc
     2581 gcagcaaaca tgcttgataa atcgattcct cgggcgacat cccttccttg cgttcggctt
     2641 cgagcgcaaa atcgatcatc atgatcgcgt tctttttcac gatgccgatg aggaggatga
     2701 tgccgataag cgcgaccaaa gaaaaatcga tgcggcaaat gagcagcgcg agcaacgcgc
     2761 cgacgccagc agatggcagt gtcgacagaa tcgtgatcgg atgaatgtaa ctttcgtaca
     2821 gaacgcctag cacgatgtaa atgaccacaa cggccgccaa aatcagcagt ggctcgctcg
     2881 ttaacgatga gcgaaattcc gccgccgcgc cggtgaatgt cgtttcgatt gtatcaggca
     2941 aaccgatttc gcgttctgtt ttttgaatcg ccggaatagc gtcactgagc gaactgccgc
     3001 tgcgcagatt aaacgaaatg gtcgccgccg gaaattgccc ttgatgcggg atggagagcg
     3061 gcgtgctgct cacgtgcatc gtcgcgaacg cgctcagcgg caccggctgg ccgctgcttg
     3121 atttgacgta aattttgtcg agcaaatcgg gcgattcgcg aaagtttggt tccacttcga
     3181 gaatgacgcg aaattgattg agctgcgtga aaatgatcga gacctgtcgc tgcccgaacg
     3241 cgtcgtaaag cgtgtcgtcg attgcttggg tgagcacgtt gaggcgcgat gctttctcgc
     3301 ggtcaacatc aatgttcaac tgcaagccgt tcacctgttg gtcgcttgcc acgtcgctga
     3361 gttgcggcag ttgtccaagt ttttgcacga gttttggcgt ccactctgcg agctcggccg
     3421 gatccgcgtc ctgcaaaatg tattggaatt gcgtgcggct gacgcgcgca tcgatctgca
     3481 aatcctgcgc cgcttgcata aagagcgaga cgccttcgac atcgcgcgtg gcattgcgca
     3541 accgttcgat gattttttcg gcattcgcgc gctgattgtg cggcttgagc acgatgtaaa
     3601 gccgggcggt attgatggtc gcgttcactg ttcccgcgcc gaccgaagcc gacacgctcg
     3661 cgacatccgg gtcgcgcctg acgatgtcgg aaacggcgtg cacgcgttgc aacatcgcct
     3721 tgaaggaaat gttctccgcg gcatcggtca cgccgacgat caccccagtg tcctgttgcg
     3781 gcagcagccc tttcggcacg acgatgtaga gaaagatggt cgcgaacagc gttatgattg
     3841 cgatatttaa gacgaatcgc tgatgcgcga acacccagcg caacccgcgt tcatacaaat
     3901 tgctaaaccg cgtccagtat tcttcggtcc ggcgatagaa ctcaccctgg tgttctcttt
     3961 cttcttcttt ttcggagcgc aacagcttcg cgcacatcat aggcgtaagc gtaagtgaaa
     4021 cgaacgccga aacaccaacc gccacactca tcgtgatcgc gaattcgcga aacaagcggc
     4081 cgacgattcc agtcatgaac aaaagcggaa tgaaaaccgc gacgagcgag atactcaacg
     4141 acaccaccgt gaaaccaatt tgcttcgccc ctttcagcgc tgcttccagc ggaccatcgc
     4201 cggcttcgat gtaacgcaca atgttctcga tcatgacgat cgcgtcatcg acgacgaagc
     4261 cggtcgagat ggtgagcgcc atcaacgata gattgtcgag gctgaatccg gccagataca
     4321 tgatgccgaa cgtcccgatg agcgagagcg gaagcgccac actcgtgatc accgtcgccc
     4381 agagttttcg caggaagagg aacatcacca ttacgacgag cgcgacggtc agtagcaatg
     4441 tgaattgcac gtcgcgcact gacgcgcgga tcgtctcggt acgat
//
LOCUS       DASTDG010000110         5811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_104574, whole genome shotgun sequence.
ACCESSION   DASTDG010000110 DASTDG010000000
VERSION     DASTDG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104574"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /gene="rplU"
                     /locus_tag="VFO30_03435"
     CDS             complement(<1..303)
                     /gene="rplU"
                     /locus_tag="VFO30_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9418369.1"
                     /translation="MPYAIIKTGGRQYRVAEGDTLDVDLLDVAPGKQATFEALLHVDG
                     DKVTFGSGKVTAEVIEQRKDKKVIAFKYKRRKGYHRTVGHRRKLTRVKIKSIGGGKK"
     gene            complement(441..1166)
                     /gene="rsmI"
                     /locus_tag="VFO30_03440"
     CDS             complement(441..1166)
                     /gene="rsmI"
                     /locus_tag="VFO30_03440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9418370.1"
                     /translation="MDGKRGFIFNPMLYVVATPIGNLGDITLRALEVLKNVDLIAAED
                     TRHSGLFLKHYKIDKPLISYHQHNEAMRTAQLVERLASGKNIALITDAGTPGLSDPGA
                     RLVRACIECQLPFTVVPGPSSILAALIGSGFSTEKFFFGGFLPNKSGGRERELRAATA
                     REETTIFFESPHRLLKTLTAATDILQDRQLCVARELTKKFEEFRRGTAAELRAHFEAH
                     PPKGEIVLLVSQKGHVPSPPSSR"
     gene            1192..1560
                     /locus_tag="VFO30_03445"
     CDS             1192..1560
                     /locus_tag="VFO30_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418371.1"
                     /translation="MRIHRYLFAFLPALLAGCAYEAPAPPVTTTTTVTREVTTTGPAT
                     TTEVIAPVAPPAVRTEVQTVAPGPNYVWQSGYWRWTGTDYVWASGGWVVRPRPMAVWV
                     AGHWVRRGTGWVWRPGHWQY"
     gene            complement(1612..4671)
                     /gene="pruA"
                     /locus_tag="VFO30_03450"
     CDS             complement(1612..4671)
                     /gene="pruA"
                     /locus_tag="VFO30_03450"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET9418372.1"
                     /translation="MASLQEQTEQRGREIFRLVDHHPQPLFSKAGFYQRLMALSMRDE
                     HFKAQMFRFVDVLPCLRRSGDIVQHLEEYFADGRNGYAPLVKAGIRAANIAPWLSAAI
                     LRRNIAGMARQFIAGKNPPNVLKTLRKRREQKIGFTVDLLGEAVLSETEADEYGARCI
                     ELLEYLARETRGWTDPLGENAELFPIVNLSVKISALYSQIDPADPADGIAHLAPKVRP
                     ILRRARELETFVNFDMESYAHKNVTLELFRTLFSEAEFRDWPHAGIVIQAYLRESQKD
                     LVDLISWGRQRGTRFAVRLVKGAYWDFETIKSQQNGWATPVFLQKPESDANFETLSRV
                     LLENESIVTPAFGSHNVRSIAAAQALAHQLGVDHSRFEFQLLYGMAGPIKRALVELGY
                     RVREYCPVGELLPGMAYLVRRLLENTSNEGFLRAKFSENVSEQELLRDPREVLVENRN
                     SALSRLNPTTTMATTLMQDRQVTRGISREIPPGDTYENAPLTNFALRENQEDMRAALR
                     DMRARLGQEYPLVINGEKISTGRTIASINPTNPEEIVGAVTEAGIPEAELAIKAARNA
                     FAKWRWTPFEERAQLLERAAGIMKRRRFELSALEVYEVGKSWTEADGDVREAIDFCLF
                     YAQQMRLIGRPRLTQYVPGEESYYHYWPRGVAFVIAPWNFPIAILCGMTTAALVTGNA
                     VIMKPAEQSAVCGAMLMEIFEEAGVPPGVLNYLPGKGSQMGAHIVDHKDVDLIAFTGS
                     REVGLRIWQSAGVTHPGQRELKRVICEMGGKNAVIIDSDADLDETIVDCISSAFGYQG
                     QKCSALSRLILLEDNYDRVMERLLSAAASVRVGNPEEPGVMVGPIIDKAAYERILDYI
                     EIGKSEATLAYQRKDVPEKGFFIPPTIFTNVKPTARIAVEEIFGPVVSVIKVRDLDEA
                     IDVANSTDYALTAGFFSRSPANIERAKAQIEAGNVYINRPCTGAVVGRHPFGGFKMSG
                     GGTKAGGGNYLLHFLVPRVVTENVTRHGFAPEETPQYRDEFLWPPRH"
     gene            4803..4934
                     /locus_tag="VFO30_03455"
     CDS             4803..4934
                     /locus_tag="VFO30_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418373.1"
                     /translation="MKGKRKIAQAGATGYILLWLLGIPIPIVADFHSAGVHVKLGSK"
     gene            5050..>5811
                     /locus_tag="VFO30_03460"
     CDS             5050..>5811
                     /locus_tag="VFO30_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9418374.1"
                     /translation="MNRRGARADFASDNTAGICPESWAALQEANTGGAESYGDDSWTA
                     RARELVQELFEIDCAVYFVLNGTAANALALAQICQSHHSVICHRYAHIQTDECGAPEF
                     FTRGAKLLLIGGANGKIDKQEAAAAIERQAQLHAQKPRALSITQATELGALYMPDEIH
                     GLAEFARSRSLLLHMDGARFANAVAAIACAPKEISWQAGVDVLCFGGTKNGTPAGELV
                     IFFRKELAHEFDYRLKQSGQLVSKMRFLAAPWIGLL"
BASE COUNT         1169 a         1617 c         1779 g         1246 t
ORIGIN      
        1 tttcttcccg ccgccgatcg atttgatctt cacccgcgtg agcttgcggc ggtggccgac
       61 ggtgcggtga taacctttgc gcctcttgta tttaaacgcg atgacctttt tgtctttgcg
      121 ctgctcgata acttccgcgg ttactttgcc gctgccgaag gtgaccttgt ccccgtcgac
      181 gtgcagcagt gcctcgaacg tcgcctgctt gccaggagcg acatcgagaa gatcgacatc
      241 cagcgtgtcg ccttccgcga cacgatactg ccgaccgccc gtcttgatga ttgcgtaagg
      301 cataccgaaa agttgtagct gccagccggt cctgccggca ggcgggaaaa gccgcgcaaa
      361 atcccataga agagtcgttc tgacaagcgg aaagcttgcc tcgttttgcc ctcgccgggc
      421 gctggcgcgt cctcctctcc tcagcgagag gatggcggtg agggcacatg tcctttctgc
      481 gaaacgagga gcacgatttc gccttttgga gggtgcgctt cgaagtgggc gcgcagctcc
      541 gcggctgtgc cgcgacggaa ctcctcgaac ttcttcgtga gctcgcgcgc gacgcacaat
      601 tgtcgatctt gcaaaatatc agtggcggcg gtgagcgtct tgaggaggcg atgaggggac
      661 tcgaagaaaa tggttgtctc ctcgcgcgca gtcgcagcgc gcaattcgcg ttcgcggccg
      721 ccgcttttgt tcggaagaaa tccgccgaag aagaatttct cggttgaaaa tccagagccg
      781 attaatgccg cgaggattga cgatggtccg ggaacgacgg tgaacggcaa ctggcactcg
      841 atgcatgcgc gcaccagtcg cgcgccggga tcggagaggc ccggcgtgcc ggcatcggtg
      901 atgagcgcga tgtttttacc gctcgcgagg cgctcgacca attgggccgt gcgcatggct
      961 tcgttgtgct ggtgatagct gatgaggggc ttgtcgatct tgtaatgctt caagaacaaa
     1021 ccggagtgtc gcgtgtcttc ggctgcgatc aggtcgacat ttttcagcac ctcgagcgcg
     1081 cgtagcgtga tatcgccgag atttccgata ggtgtcgcga cgacgtagag cattgggttg
     1141 aaaataaagc cgcgttttcc gtccaatccg aatgaaaaga gaggaaccgc tatgagaata
     1201 catcgttatc tgtttgcttt tttaccggcg ttgctggccg gctgtgcgta tgaagcgccg
     1261 gcaccgccag tgaccaccac gacaaccgta acgcgtgaag tgacgacaac tgggccagca
     1321 acgaccacag aagtgatcgc cccggtagcg ccgcccgccg tgcgcaccga agtccaaacg
     1381 gtcgcgcccg gcccaaacta tgtctggcag tccggctact ggcgctggac aggaacggat
     1441 tacgtgtggg catcaggcgg atgggtcgtt cgcccccggc caatggctgt ttgggtggcg
     1501 ggacattggg tccgtcgcgg gaccggctgg gtctggaggc ccggacattg gcagtactaa
     1561 acgtactctc cagtcagtga cgtgggatgc cgcggtgcgg cgtcccaact tttagtgccg
     1621 cggcggccag agaaattcgt cccgatactg cggcgtctct tccggcgcga aaccgtgtcg
     1681 cgtgacgttt tcggtcacga cacgcggcac gagaaagtgg agcagataat tgccgccgcc
     1741 ggcttttgtg ccgccgccac tcattttaaa tccaccgaag ggatggcggc cgacgactgc
     1801 gccggtgcac ggccgattga tgtacacgtt gccggcttcg atctgtgctt tggcacgctc
     1861 gatgttcgcc ggactccgcg aaaagaatcc agcggtgagc gcgtaatcgg tgctgttcgc
     1921 tacgtcgatt gcttcatcca agtcacgcac tttaataaca ctcacgaccg gcccgaagat
     1981 ttcctcgacc gcgatgcgtg cggtcggttt cacatttgtg aaaatagtcg gcggaatgaa
     2041 gaagcctttc tcgggcacgt cttttcgttg ataggcgagt gtggcttcgg atttgccgat
     2101 ctcgatgtaa tcgagaatgc gctcgtaagc ggccttgtcg atgattgggc cgaccatgac
     2161 gccgggttct tccggattgc cgacacgtac actcgcggcg gcgctgagca accgttccat
     2221 gacgcggtcg taattgtctt cgagcaaaat caagcgcgat agcgcagaac acttttgccc
     2281 ttgataaccg aaagcggagg agatgcagtc aacgatcgtt tcgtcgaggt cagcgtcgga
     2341 atcgatgatg acagcgtttt tgcctcccat ttcgcaaatg acgcgcttga gctcgcgctg
     2401 accgggatgc gtcaccccgg cgctttgcca gatgcgcagc ccgacttcgc gcgaaccggt
     2461 gaaagcgatg agatcgacat ctttatgatc gacaatatgc gcgcccatct gtgagccctt
     2521 gccgggcaga taatttagga cgcccggcgg cacgccggct tcttcgaaaa tctccatcag
     2581 catcgcgccg caaacggccg attgctcggc cggcttcatg atgacggcat taccggtcac
     2641 gagcgcggcg gtggtcatgc cacaaaggat cgcgatagga aaattccaag gcgcgatgac
     2701 aaaagcgacg ccgcgcggcc agtaatgata atagctctct tcgcccggga cgtattgcgt
     2761 cagccgcggc cgcccgatga gccgcatctg ctgcgcgtaa aagagacaaa agtcgatcgc
     2821 ctcgcggaca tcgccgtcgg cttcggtcca cgatttgccg acctcgtaaa cctcgagggc
     2881 ggacagctcg aagcggcggc gcttcatgat cccggcggcg cgttcgagca actgcgcccg
     2941 ctcttcaaac ggtgtccacc gccactttgc gaacgcgtta cgcgcagctt tgatcgcaag
     3001 ttctgcttcg ggaatgccgg cttcagtgac tgcaccgacg atctcctcgg gattcgtcgg
     3061 gttgattgag gcaatggttc tacctgtcga aattttttca ccattgatga cgagcgggta
     3121 ttcctggccg agtcgcgcgc gcatgtcgcg caatgcggcg cgcatgtcct cctggttttc
     3181 gcgtaaggcg aagttggtta gcggtgcatt ttcgtaagtg tcgccaggtg gtatttcgcg
     3241 ggaaatgccg cgtgttacct gtcgatcttg catcagtgtt gtcgccatag tcgtggtggg
     3301 gtttaagcgc gatagagcgc tgtttcggtt ctcaactaaa acttcgcgtg gatcgcgcag
     3361 caattcctgc tcggaaacat tctccgaaaa ttttgcgcgc aaaaatcctt cattcgatgt
     3421 gttctcgagc aagcggcgaa cgaggtaggc catgcccggt aacagctcgc caaccggaca
     3481 atattcacgg acgcggtagc cgagctcgac gagcgcgcgc ttgattgggc cggccatgcc
     3541 gtagagcaac tggaactcga accggctgtg gtcgacgccg agttggtggg cgagcgcctg
     3601 cgccgctgcg atgctgcgga cgttgtgcga accgaatgcc ggagtaacga tcgattcatt
     3661 ttccagaagt acgcggctga gggtctcgaa gttcgcgtcg ctctcgggct tttgcagaaa
     3721 caccggcgtc gcccagccgt tttgttgcga tttgatcgtt tcgaagtccc aatacgcgcc
     3781 tttgaccagg cgcacggcaa aacgcgtccc gcgctgccgg ccccagctaa taagatcgac
     3841 aagatccttt tgcgattcgc gcaagtatgc ctggatcacg atgccagcat gcggccaatc
     3901 gcgaaactcc gcctcgctga atagcgttcg gaaaagctcg agcgtaacgt ttttgtgcgc
     3961 gtaactttcc atgtcgaaat tgacgaaggt ctcgagctcg cgcgcgcggc gtaaaatcgg
     4021 ccgcactttc ggcgcgagat gggcgattcc atcggcggga tcggccggat ctatttgcga
     4081 atagagcgcg gagattttga cggagagatt cacaatggga aacagctccg cgttttcgcc
     4141 gagcgggtcg gtccagccgc gcgtttcgcg cgcaagatat tcgagtaact cgatgcagcg
     4201 ggcgccgtat tcgtccgctt ctgtttcgct caacactgct tcgccgagca gatcgacggt
     4261 gaagccgatt ttttgttcgc gccgtttgcg caatgttttc aggacattcg gcgggttttt
     4321 tccggcgatg aactggcgtg ccatgccggc aatgttgcgg cggaggatgg ccgcgctgag
     4381 ccacggcgca atattggcgg cgcggatgcc agctttcaca agcggcgcat agccattgcg
     4441 cccgtcagca aaatattcct ccagatgctg gacgatgtcg ccactgcggc gcaggcaagg
     4501 cagaacatca acaaagcgaa acatttgcgc cttgaaatgt tcgtcccgca tcgagagggc
     4561 catgagccgc tggtaaaagc ccgctttgct gaagagcggt tgcgggtggt gatcaaccag
     4621 gcggaaaatc tcgcggccgc gttgctccgt ttgctcctgg agagaggcca ttgaatgagc
     4681 atactgctgc ggagtgggtc ggcaaaggcg attgcggtcg gcacaaagtt tttacgtttc
     4741 gcacacactc aaccgccacc cctattccga aagtagggat gaaccgcaga aaaggacaag
     4801 caatgaaagg gaaaaggaaa atcgcgcagg ccggggcgac tggttacatc ctactttggc
     4861 tactcggtat tccgattccg attgttgctg attttcattc tgcgggggtg cacgtgaagt
     4921 tggggagcaa gtagaggtga tttgcctggc ggacaccgga ggcccagggc tcgggtctgt
     4981 caggcaatct ctacgtcggg gagctgttgc tgttgcttag caggagcaat tcgcgtagca
     5041 acaggcgaga tgaaccgccg cggggcgcgt gctgattttg ccagcgataa tacggcggga
     5101 atttgcccgg aatcttgggc cgcgctgcag gaagcgaaca cgggcggggc agaatcgtac
     5161 ggcgacgaca gctggacggc gcgtgcccgt gaactcgtgc aggagttatt tgaaatcgat
     5221 tgcgccgttt acttcgtgct caacggcacg gcagccaacg cgcttgctct ggcgcagatt
     5281 tgtcagtcac atcacagcgt catctgccac cgctatgctc acatccaaac cgacgaatgt
     5341 ggcgcgccgg agttttttac cagaggggca aagctccttc tgatcggcgg ggcgaatggt
     5401 aagatcgaca agcaggaagc cgcggcggca atcgagcggc aagcccaatt gcatgcgcaa
     5461 aaaccccgcg cactcagcat cacccaggcg acggaactcg gcgcccttta catgcctgac
     5521 gagattcacg gacttgccga gttcgcgcga agccgatcac tgcttctgca catggacggc
     5581 gcgcgctttg cgaacgctgt cgcagcgatc gcgtgtgcgc cgaaggaaat ctcatggcaa
     5641 gccggggttg atgttctttg cttcggcgga acaaagaatg gaaccccggc aggcgagctt
     5701 gtgatttttt ttcgcaaaga actcgcgcac gagttcgatt accggttgaa acaaagcggc
     5761 caactcgtgt caaaaatgag gttcctggct gcgccgtgga tcggtttgtt g
//
LOCUS       DASTDG010000111         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1211098, whole genome shotgun sequence.
ACCESSION   DASTDG010000111 DASTDG010000000
VERSION     DASTDG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211098"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFO30_03465"
     CDS             <1..278
                     /locus_tag="VFO30_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9418375.1"
                     /translation="LADLPGMRVLGPSGQRGAIVGFVMDSVHPHDLTTFADQRGLALR
                     GGHHCNQPLMRKFNLPGTARASFYFYNTKEEVDRMIDILRDAVRFFS"
     gene            275..757
                     /locus_tag="VFO30_03470"
     CDS             275..757
                     /locus_tag="VFO30_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9418376.1"
                     /translation="MSIELEELYQEVILDHSRRPRNFGELPDAAVVVHGDNPACGDEI
                     HLAVRFGDDGSLQDIKFTGHGCAISQASASMMTTKLKGKSRDETMSILHKFRDLVTGD
                     GETPPEARKLGDLQLMQGVRKFPQRVKCAMLAWRAVEQALQQTVGEATVSTEVDDPLT
                     "
     gene            complement(760..>2359)
                     /locus_tag="VFO30_03475"
     CDS             complement(760..>2359)
                     /locus_tag="VFO30_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET9418377.1"
                     /translation="RPATFLRLFLSPHFCEARRAMLAKIYSAALYGVDAYEVEVEVNG
                     AHGDPKIFIVGLPDTAVKESLHRVTTAVNNSGYRWPRGRTTVNLAPADIKKEGPSFDL
                     PIALGMISVTEELDSSPFENFSFVGELALDGTVRAVKGVLPVALEARRRGKRGLFVPQ
                     ANALEAAMVEKIDIYGVQNLRQTFDFLRGEIALLPTRADLTKFFATHQDYDVDFSDVK
                     GQGHVKRAIEVAVAGGHNILMIGPPGSGKSMLAKRIPTIIPPMTLAEAIETTKIHSIA
                     GLLNGARSFVATRPFRSPHHTISDIGLLGGGTFPNPGEVSLAHNGVLFLDELPEFKRS
                     ALEVMRQPLEDGKVTVSRAAGSVTFPSELMLIAAMNPCPCGYFGDLKRECRCGPLQVQ
                     RYRQRISGPLLDRIDLHIEVPPVEYRDVSSQRIEESSSAIRERVGRARNIQQQRFAGV
                     DGKTNCNARMTTRHLKQFCKLSQESQDLIRVAMNELNLSARAYDRILKVARTIADLDG
                     KIDVLPEHVSEAIQYRTFDRTLWV"
BASE COUNT          474 a          679 c          721 g          485 t
ORIGIN      
        1 aattggccga cttgcccggc atgcgcgtgc ttggaccgtc tggccagcgc ggagcgatcg
       61 tcggcttcgt catggattcg gtgcatccgc acgacctcac aacatttgcc gatcagcgcg
      121 ggctcgcctt gcgcggcggc catcattgca accagccgtt gatgcgaaag ttcaatctgc
      181 cgggcacggc acgcgcgagt ttctatttct ataacacgaa agaagaagtc gatcgcatga
      241 tcgacatcct gcgcgacgcg gtgcgtttct tctcatgagc atcgaactcg aagaactgta
      301 tcaggaagtc atcctcgatc attcccggcg gccgcggaat ttcggcgaac tgcccgatgc
      361 cgcggttgtc gtccacggcg ataatccagc gtgcggggat gaaattcatc tggcggttcg
      421 cttcggggac gacggctcac tgcaagacat caaatttacc ggccacggct gcgccatcag
      481 ccaagcgtcg gcatcaatga tgaccacgaa gctcaaggga aaaagccgag atgagacgat
      541 gtcgatcttg cacaagtttc gcgatctggt gaccggcgac ggcgagacac cacccgaagc
      601 gcgtaaactc ggcgatcttc agttgatgca aggtgtccgc aaattcccgc agcgcgtgaa
      661 atgcgccatg ctcgcctggc gcgccgttga gcaggcgctg caacaaaccg tcggcgaagc
      721 caccgtttcg accgaagtgg acgatccgct cacctgagct cagacccaga gcgtgcgatc
      781 gaaggtgcga tactggatcg cttcactgac gtgctcgggc aggacgtcga tcttgccgtc
      841 gaggtcggcg atcgtgcgcg cgactttcag gatgcggtcg taagcgcgcg cgctgaggtt
      901 gagctcgttc atggccacac gaattagatc ctgcgattcc tggctgagtt tacaaaactg
      961 tttcaggtga cgtgtagtca tccgggcgtt gcagttcgtt ttgccgtcaa cgccggcgaa
     1021 acgctgctgt tggatgttgc gcgcacggcc gacgcgctcg cgaatggccg aggaagactc
     1081 ttcgatacgt tggctcgaga cgtcacgata ttcgactggc ggcacttcga tgtggagatc
     1141 gattcggtcg agcagcggcc cggaaatgcg ttgccggtag cgctgaactt gcagcggtcc
     1201 gcagcggcac tcgcgtttca ggtcgccgaa gtagccgcac gggcacgggt tcatcgccgc
     1261 gatgagcatc aattcactcg ggaacgtaac gctgccagct gcgcgtgaaa ccgttacttt
     1321 gccgtcttcc agcggctggc gcataacttc caaggcggag cgtttgaatt cgggaagctc
     1381 atcgagaaaa aggacgccgt tgtgcgcgag gctcacttcg cccggattcg gaaacgtgcc
     1441 gccgccgagc agaccgatgt cgctgatggt gtgatgcggt gaacgaaacg gacgcgtcgc
     1501 gacgaatgac cgcgcgccat tcagtaaccc ggcgatgcta tgaatcttgg tcgtctcgat
     1561 cgcttcagcc agcgtcatgg gaggaatgat ggtcgggatt cgtttcgcca gcatcgattt
     1621 acctgacccg ggcggaccga tcatgaggat gttgtgaccg ccagcaacgg cgacttcgat
     1681 ggcgcgtttg acatgtccct gtcctttcac atcgctgaag tcgacgtcgt aatcctggtg
     1741 ggtggcgaag aatttggtga gatcggcacg agtgggcagc agcgcgattt cgcctcgcag
     1801 aaaatcgaac gtctggcgga ggttttgcac cccgtagatg tcgatcttct cgaccatcgc
     1861 tgcctcgagc gcgttggcct gaggaacgaa taaccctcgc tttccacggc ggcgcgcttc
     1921 gagtgccacg ggcaacaccc ctttcacagc gcggactgtc ccgtcgagcg cgagctcgcc
     1981 aacgaagctg aagttttcga atggggaact gtcgagttcc tcggttacgg agatcatacc
     2041 gagcgcgatc ggtaagtcga agctcggtcc ttcttttttg atgtccgcag gtgcgagatt
     2101 gacggtcgtg cgaccgcgtg gccagcgata gccgctgttg ttcacggcgg tcgtgacacg
     2161 gtgcagactt tccttcaccg ccgtgtcggg caaacctacg atgaaaattt tcggatcacc
     2221 gtgcgcgccg ttcacttcca cttcgacttc gtaagcatcg acgccgtaaa gcgcggccga
     2281 gtagatcttc gccagcatcg cccgccgagc ttcgcaaaaa tgcggcgaga ggaaaaggcg
     2341 caaaaacgtc gccggcctg
//
LOCUS       DASTDG010000112         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_401727, whole genome shotgun sequence.
ACCESSION   DASTDG010000112 DASTDG010000000
VERSION     DASTDG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401727"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..536)
                     /locus_tag="VFO30_03480"
     CDS             complement(111..536)
                     /locus_tag="VFO30_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418378.1"
                     /translation="MMIRNRTSAFVTTAALAAICGVVAAKEKTGGGKTSAGMAEYQRG
                     IQLFDNKQYDQAIVEFSKAILANEKEPAFYENRGFTYLAMQMPGEAAADFSKVIELAP
                     KEEPAYVGRAQALIQQKIYDQALGDLEQAFRLKPDDPLA"
     gene            619..1596
                     /locus_tag="VFO30_03485"
     CDS             619..1596
                     /locus_tag="VFO30_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9418379.1"
                     /translation="MKQPAAVGGIVDLRGRANLSRGYAALFATVVIWSMPSLFQYYLI
                     RYYDPWAQNFYRYLAACLAVVPFVFLHLRRPATAGKIDVHAFAICLIPCLPNVIHQIT
                     QVVALYYIGPGVYAIFTRSSVIFTALLALIFFPEERYVIRQWQFQTGTLLGLLGAFGV
                     VWFQTGWQTGPIPFRGLVIAFTATFCWALYSILVKRPSAQLGSIRSFGVISLITSTLL
                     LPLTLLFGNIDTPLRVGANVNVILVVSAVSCITLAHVLYYVAIQEIGVALAQSLQLLC
                     PAGALALSAWIFGERLTYPQLWSAAILLLGAFLAMQTKPGATTETAENL"
     gene            complement(1734..3398)
                     /locus_tag="VFO30_03490"
     CDS             complement(1734..3398)
                     /locus_tag="VFO30_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HET9418380.1"
                     /translation="MKTTATTTSFPNELSLSHRRIVSQLLGLPVTLLLVFLSVNTSVA
                     GSATWNLNPIRGDWNTAANWTPMTVPNGTFPFGPFDIATFARSNITSVSLSAEVNVNS
                     IVFVPNASAFTINTQSQLLRLEGAGILNSSGTTQNIVAGRPFGLIGFFNNSSAGTNVT
                     YTVEPDPTNGQGFGGAIIFGDSSTAGDATFIFPENTLSNGDGGFVLFEESATAGTAEF
                     IVSGGVGSGAHGARVQFFNTSNADQATFTINGGKGSDFLGGGELRFSQASGAGTATLI
                     ANGGSFAPGGLIIFEDHAHGGTAQVKVFGNGTLDISAQKDGSVTIGSIEGDGDVFLGG
                     AQLVVGRNDLNAVFSGAIQNGGSNGGVGGSLVKIGGGKLVLAGANTYSGGTRVMHGQL
                     FVNNPRRSGTGSGTVLVDGGKLGGNGFIGAGVRIGTGSGTGATLAPGGAVGSTGTLEI
                     KRALTFNSDGIYQVQVNSSNVTADEVVANGVTLNAGAQFAFADLGNGTLPLGTAFTII
                     GNTAATPIAGRFSNLADGLVFTANGNTYRVDYKGGDGNDLTLTVVP"
     gene            3920..4801
                     /locus_tag="VFO30_03495"
     CDS             3920..4801
                     /locus_tag="VFO30_03495"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET9418381.1"
                     /translation="MDLKSYLASRQKLIDRALDRYLPNEKTRPTTIHKAMRYSLFAGG
                     KRLRPILCLAAAETCSGKNDHALPLACALECIHTYSLVHDDLPSMDNDDFRRGRPTCH
                     KVFGDGIAVLAGDALLTVAFEIVAHAKTTPRYDISILLREIAVAAGSERLIAGQVADL
                     EAEGKNVNRAQLRYIHENKTAAILTTSVRLGAMSANANRIRLNAMTKFGRALGLAFQI
                     IDDILDVTQTTEKLGKSAGKDVAAQKATYPAVIGLEASRNEARRLTGAAHDALSIFGQ
                     KAEVLHALANYLLEREY"
     gene            complement(4850..5830)
                     /gene="tsaD"
                     /locus_tag="VFO30_03500"
     CDS             complement(4850..5830)
                     /gene="tsaD"
                     /locus_tag="VFO30_03500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9418382.1"
                     /translation="MTILALETSCDETALAILHGNKLLASEVASQIAEHEQYGGVVPE
                     VASRNHLVQAPRLLERATRAAKIDISQVDAFAATSGPGLASSLMIGASIAKGLAIGFG
                     KRYLAINHLEGHLLSPFFPEAEIRPNVSLIVSGGHTMSIMVRGVADYQVIGHTVDDAA
                     GEAFDKVAKLLGLGYPGGPEIEKHARSGNPTGFDLPRSMPDSENFSFSGLKTAVRYLL
                     PKLRGDYLADLCASFQQAIVDVLVEKTMAAAKKHEVDLVTVSGGVSCNKELRRQLQEA
                     CKRDGFELKSAQPWLCTDNAAMIAFAASLRLQNGFESSVTEEIDPNLKVA"
BASE COUNT         1196 a         1821 c         1584 g         1324 t
ORIGIN      
        1 cgccggtcgt agttttgcag gtcatttgga cttttttcta tcgcggtcgt gaaatctgcg
       61 accgccttgt cccattgcgt cagcccaacc tcagcaaagc cgcgaaactt ctacgcgagc
      121 gggtcatccg gtttcagtcg aaaggcctgc tcgagatcac caagcgcctg atcgtagatc
      181 ttctgctgaa tcaaagcttg cgcgcgtccg acataggcag gctcctcctt tggtgccagt
      241 tcgatcacct tcgaaaaatc ggctgctgct tcgcctggca tctgcatggc caaataagtg
      301 aagccccggt tctcataaaa tgccggctcc ttttcattgg cgaggatcgc tttgctgaac
      361 tccacgatcg cctggtcgta ctgcttgttg tcaaaaagtt gaatgccgcg ttggtattcg
      421 gccataccgg cgctcgtctt gccgccgccg gtcttttctt tggctgcgac gacgccgcag
      481 atcgccgcaa gtgccgcggt ggtaacaaat gcactcgttc gatttctgat catcatattc
      541 gtctcctttg cttttatttt ttgagcgatt aatcgggccg aagcgagcaa attaacgcgc
      601 cccgttctga atcacctgat gaaacaaccc gctgcagttg gtggaattgt cgatctgcgc
      661 ggacgcgcca atctctcgcg cggttacgcc gcgctcttcg ccaccgttgt catctggtca
      721 atgccgtcgc tctttcagta ctatctcatc cgctactacg atccttgggc gcaaaatttt
      781 taccgttacc tcgccgcttg cctcgctgtc gtgcccttcg tgttcctgca tctgcgccgt
      841 cccgcgactg cgggaaagat cgacgtgcac gcgttcgcca tttgcctcat cccgtgcctg
      901 ccgaatgtca tccaccagat cactcaggtc gtcgcgctct actacattgg gccgggcgtc
      961 tacgccatct tcacccgctc atcggtcatt ttcaccgcgc tgctcgcgct gatcttcttt
     1021 cccgaagaac gctatgtcat tcgccagtgg cagttccaaa ccggcacgtt gcttggcctg
     1081 ctcggcgcgt tcggcgtcgt ttggttccaa actggctggc aaaccgggcc gatcccgttc
     1141 cgcggattgg ttatcgcatt cactgccaca ttctgctggg cgctttacag tatcctcgtg
     1201 aaacgcccgt ccgcacagct cggctccatc cgcagttttg gcgtaatcag cctcatcact
     1261 tccaccttgc tgctgccgct cactcttctc ttcggcaaca tcgacactcc gctgcgcgtc
     1321 ggcgccaacg tgaatgtcat tctcgtcgta tccgccgtga gctgcatcac gctggcgcac
     1381 gtcttgtatt acgtcgccat ccaggagatc ggtgttgcgc tcgcgcagtc cctccagctg
     1441 ctttgtccag ccggtgccct cgccctgtcg gcgtggatct ttggcgaacg cctcacctat
     1501 ccgcaacttt ggagtgccgc catcctactc ctcggcgcct ttctcgccat gcaaaccaaa
     1561 ccaggagcga ccaccgaaac agccgagaat ctgtaggttg ccaagcgtcg tcttaccttc
     1621 cagtgccaat caggaaagca ggaacgaagc ggaagtgaca ctgctctagg tctctctgct
     1681 acttttctaa tttggaaagc tattccccgt tttctggctt ccaaattcag cactcagggc
     1741 acgactgtaa gcgtaaggtc gttgccatcg ccgcctttgt agtccacccg gtaagtgttg
     1801 ccgttggccg tgaagaccag accatcagca agattgctga accttcccgc gataggcgtt
     1861 gccgcggtgt tgccgatgat cgtgaaagcg gtgccaagcg gaagcgtgcc gttcccgaga
     1921 tcggcaaagg caaattgcgc gccagcattg agggttacgc cgttggctac tacttcatcg
     1981 gccgtaacat tgctgctatt cacctgcact tggtaaatac cgtcggagtt gaatgtaagc
     2041 gccctcttta tctcaagcgt cccggtacta ccaactgctc cacccggcgc cagagtcgcg
     2101 ccagtgccac tccccgtgcc gatccgtacg ccggcgccaa tgaacccatt gccgccaagt
     2161 ttgccaccgt cgactagaac ggtgccagag ccggtgccgg accgacgggg gttgttaacg
     2221 aaaagctgac catgcatgac ccgcgttccc ccagagtatg tgttcgcacc ggcgagaacc
     2281 agcttgcccc caccaatctt cacgagcgag ccgccgacac cgccatttga tccgccattc
     2341 tggatcgcgc cggagaagac tgcgtttagg tcattgcggc ccacgaccaa ttgagcgccg
     2401 cccaggaaaa catccccgtc tccctcaatc gacccaatcg tcacgctccc gtctttctgt
     2461 gcgctaatgt ccagagtgcc gttgccgaac acctttacct gcgccgtgcc gccgtgggca
     2521 tgatcctcga aaatgattaa accgccgggg gcgaatgagc ctccattcgc gatcaatgtc
     2581 gcagtgcctg cccccgaagc ctgggagaag cgaagctcgc cgccccccaa gaagtcgctg
     2641 ccctttccac cattgatcgt aaaggtcgct tggtcggcgt tcgatgtatt aaagaattgg
     2701 acccttgctc catgcgcccc gctgcccaca ccgccgctaa caatgaactc cgcagtgccc
     2761 gccgtggcag attcttcgaa cagaacaaat ccaccatcgc cattgctcag cgtgttctcg
     2821 gggaagatga aggtggcatc tccggcagtg gatgaatcac cgaatatgat ggcgccgccg
     2881 aagccctgcc cattggttgg atctggctca accgtgtagg tcacattggt gcccgcgctt
     2941 gagttgttga agaagccgat cagaccaaag ggcctcccgg cgacaatgtt ttgcgtagtg
     3001 cctgaactgt taagtatgcc ggcgccttca agtcgcaaca gctgcgactg agtgttgatg
     3061 gtgaatgcgc tggcgttcgg aacgaacacg atgctgttca cattcacttc ggcggaaagg
     3121 gacactgacg tgatgttgga ccgcgcaaac gtcgcgatgt cgaacggccc gaacggaaac
     3181 gtcccgttgg gtactgtcat tggcgtccag ttcgccgccg tgttccagtc acctctgatc
     3241 gggttgagat tccatgttgc gctgccggct acgctggtgt tcaccgataa gaacacgagc
     3301 aacagagtga ccggcagacc aagcaactgc gaaacaatgc gccgatgcga caaggatagc
     3361 tcgttaggga aggaggtggt cgttgcggta gttttcatat tttgctctcg attgaatgac
     3421 cgcagccatt ccggattgtg cgcgctcatc tggtatttgt aatgagatgc gcgtgacccg
     3481 atgcaggact gcggcccgtt tcgtggagcc tgctcttaag caaatgtgat tagcaagaaa
     3541 aaactagagc cattagagcc gaatcgctcc tcttgttcga ggcggcgacg ccgaccgccg
     3601 gaagtaaagc tcgctccgga gcaggatgcg ttcgggcaac cactgttgct ttcaccgccc
     3661 ctccggtttg atacaagttg gcaacgtatg cagccgctcg cgcctggagt cggaacggtc
     3721 gcgctcgttt cattctcttt tttttgcacc tcggatgggc cgtatacgac tgagacgccg
     3781 attcattcgg tccggacccg ggctcggcct cgagagcaag aacaatagtc ccgcggcaaa
     3841 tagttttgca gcttggcgct taactccaaa acctttcgcg agttgacaca agcaccgcta
     3901 caatgcgcca tttcggatcg tggacctgaa gagttatctc gcatcacgcc agaagcttat
     3961 cgatcgcgcg ttagatcgct atctgcctaa cgagaagacg cgccctacga ccattcacaa
     4021 ggcgatgcgc tatagcctgt ttgccggcgg aaaacggttg cggccgatct tatgtctcgc
     4081 tgcggcggaa acctgcagcg gcaagaacga ccatgcattg cccctcgcgt gcgccctcga
     4141 gtgcattcac acttattccc tcgtccacga tgatctgccg agcatggaca atgacgattt
     4201 ccgccgcggg cgcccgactt gccacaaagt ctttggcgac ggcatcgccg tactcgccgg
     4261 tgatgcactg cttacggttg cgtttgaaat cgtggcgcac gccaaaacga cgccgcgtta
     4321 tgacatctcg atcctattgc gcgaaattgc cgttgccgcc ggcagcgaga gactaatcgc
     4381 tggacaggtg gccgatctcg aggccgaagg caaaaacgtg aaccgagcgc agcttcgcta
     4441 catccacgaa aacaaaaccg ccgccatcct gaccacgtct gtccggctcg gagcgatgag
     4501 cgcaaacgcc aatcgtatcc gactgaatgc gatgacgaaa ttcggacgcg cgctcgggct
     4561 cgcattccaa atcatcgacg acattctcga cgtcacacaa acaaccgaga aactgggcaa
     4621 aagcgcgggc aaagatgtcg ccgcgcagaa ggccacttat ccggccgtga tcggtctcga
     4681 ggcttcgcgg aatgaagcgc gacggctcac cggcgcagcg cacgatgcgt tatcgatctt
     4741 cggccaaaaa gccgaggttc tgcacgcgct cgcgaactac ctgttggagc gcgagtatta
     4801 gaatagcgcg accatcccgg ttgcgatctt caccgcgcaa gcgaaacgct tacgccactt
     4861 tcaggttcgg gtcgatctct tccgttacgc tcgattcgaa gccgttctgt aagcgcagcg
     4921 aagcggcaaa cgcgatcatg gcagcgttat ccgtgcaaag ccacggctgg gcgctcttga
     4981 gctcgaatcc gtcgcgcttg cacgcctctt gcaattgacg tcgtaactct ttgttgcaac
     5041 tcacgccccc gctgacggtc acaagatcga cctcgtgctt tttggccgcg gccatcgtct
     5101 tctcaaccag aacatcaacg atcgcctgct gaaacgacgc gcaaagatca gctaaataat
     5161 cgccgcgcag ttttggcagc aggtagcgca cggcggtttt taatccgctg aaactgaaat
     5221 tctccgaatc cggcatgctg cgcggcaaat cgaaacctgt cgggttgccg ctgcgggcgt
     5281 gcttttcgat ttcgggtccg ccggggtagc cgagaccgag cagcttggcc actttgtcga
     5341 acgcctcgcc ggcggcatcg tcgaccgtgt ggccaataac ttgataatcg gcgacaccgc
     5401 gcaccatgat cgacatcgtg tgaccgccgc tgacgatgag ggagacgttc ggtctgattt
     5461 ccgcctcggg aaaaaatggc gagagcaaat ggccttcgag atgattgatc gcgagataac
     5521 gtttgccgaa accgatcgcg agacctttgg caatcgacgc gccgatcatc aacgaactgg
     5581 ccaagccggg tccgctcgtc gccgcgaacg cgtcgacttg ggagatgtcg atcttagcgg
     5641 cgcgcgtggc acgctcgagc agccgcggcg cttgcacgag atgattgcgt gacgccactt
     5701 ccggcacaac cccgccgtat tgttcgtgct cggcgatctg cgaagcaact tcactggcca
     5761 gcagtttgtt gccgtgcaga atcgcgagtg ccgtttcgtc gcatgaagtc tccagcgcca
     5821 gaattgtcat cgcgcattgt agcggcgctc atatcaagcg ccgaagctaa ttagacaggc
     5881 gcttggcaca agcgcctcta caattatttt tttaccgact ccggc
//
LOCUS       DASTDG010000113         9246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1543435, whole genome shotgun sequence.
ACCESSION   DASTDG010000113 DASTDG010000000
VERSION     DASTDG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543435"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFO30_03505"
     CDS             <1..1278
                     /locus_tag="VFO30_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9418383.1"
                     /translation="EITGAINCLYDITERKYAEQQQEALYQFVQRQSLAASVRDIYDA
                     ALEAIVLTMRCDRASILLFDHHDVMRFVAWRGLSKAYRKAVEGHSPWKRDVEDPHPVC
                     IEHVDLARLPKPLKTTIKAEGITAAAFIPLVANGKLFGKFMAYYDQPHVFTEKEMTLA
                     VSIARQLTLGLERKRAEEELRKSKLLLEQRVQQRTEALRAANRELQREIERRKGLEGE
                     ILEVSDREQQRLGQELHDGLCQQLAAIGFMARATALRVKDHRVVQVEDLEKIAQLVNA
                     SVSDARNIARDLHKEEVDAAGFVEALRSLTQREMWKTRCRFELKTDVHLEDDRTASQL
                     YRILREALINAHKHAQASQIVLEVRQMKTNLVFSVTDNGVGFSRKTKTGRGLGFHIMQ
                     YRARSVGARLKLESPAKRGARVVCYLPHPNDAR"
     gene            1305..2009
                     /locus_tag="VFO30_03510"
     CDS             1305..2009
                     /locus_tag="VFO30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9418384.1"
                     /translation="MKLAASNLEAPEAAAPAKKKRIIIVDDHPLFRKGLEQLINSDGG
                     LTVCGEAGDASAAMTVIREKRPDLVIVDLSLPGANGIELIKNIRAEFHKVPVLTLSMH
                     DESLYALRALRAGSQGYVMKQEALENVITAIREVFAGRPYLSPAMSATVIAKFAQRSS
                     GDVSANLVDRLSDRELEILELIGKGRDVREIAKMLHLSPKTVETHRAHIKEKLSLANA
                     RQVARFAVQWVGQKGV"
     gene            2224..2430
                     /locus_tag="VFO30_03515"
     CDS             2224..2430
                     /locus_tag="VFO30_03515"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET9418385.1"
                     /translation="MAKIVKVIEVLSESPTSWEEATQNVVTEAAKTLRNIRSVYIKEL
                     TAAVENDKVTSYRVNAKVTFEMER"
     gene            complement(2503..2736)
                     /locus_tag="VFO30_03520"
     CDS             complement(2503..2736)
                     /locus_tag="VFO30_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418386.1"
                     /translation="MAKCELCGNDYDKTFELRKDGKTHIFDSFECAIAVMAPECEHCG
                     CKIVGHGVEAGGKIFCCAHCARESGENKLRDRG"
     gene            complement(2864..4321)
                     /gene="lysS"
                     /locus_tag="VFO30_03525"
     CDS             complement(2864..4321)
                     /gene="lysS"
                     /locus_tag="VFO30_03525"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9418387.1"
                     /translation="MIDEENELIVLRRQKLDALRARGIEPFGNAFEISGDIAEIRAKF
                     TEGETVRVAGRVTAHRDMGQSHFVDLRDATGRIQIYIQTKEVGPEIVELFPLIDVGDF
                     IGVEGTCFVTKTGEPTIRLGRLAVLAKSLRPLPEKWHGLQDIEARYRQRYLDLLTNER
                     SREIFLKRIAMVRETRHFLENRGFLEVETPILQTVAGGAAAQPFQTHHKALGLDLYLR
                     IAPELFLKQLLVGGFNKVFELNRNFRNEGISRKHNPEFTMLEIYWAYADFEKIANLVE
                     ELICHVAEKVCGSVQLEHRDADGRIVRTINLQRPWRRARYVDLVGDVAGEDWFDLSPD
                     ERRARATREFKLEVLPQLVDYEVTQHVFEKLVEEKTFDPLFVTHCPKELVPLARQNRE
                     DQSLVDVFELIINGAEIAPGYSELNDPQVQRRRLLEQAGEETQKIDEDFLLALEHGMP
                     PAGGVGIGIDRLTMLLTGAESIRDVILFPLLKPKK"
     gene            4584..5054
                     /locus_tag="VFO30_03530"
     CDS             4584..5054
                     /locus_tag="VFO30_03530"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418388.1"
                     /translation="MPLDLPETTSRLGGPLVRQFSVFLPNKVGALLDILKLLNTHNTH
                     VVAMSVSESTDSAIARVVVSDPEQVEELFRAHDVAFGVSEVLVVEMREVATQLASLFA
                     ALLKAEVNVHFSYAFLTRPRGLAALALHVDDVECASSVITGDGFKLLSQDDISR"
     gene            5151..6221
                     /locus_tag="VFO30_03535"
     CDS             5151..6221
                     /locus_tag="VFO30_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418389.1"
                     /translation="MRTFSISSPVTADRSFIVALGFVAFFAAAEICAVAYHYVGPVRI
                     GAATAPASVAVTRTPAPAPAATAAPAAASSAAPLSPSDGAIKAAIALRDRGDTANALA
                     KLQTASEQDPSNPNILEELARTYEAVQNVELANVTWRKIQELGPAAGSSFALADQRLK
                     AGLATPAASGAGLPGPARLDTASSEPAAPPSTDTSVLTISDVQITETPDPDADTNLTV
                     RVGIQKRTNTVIDHTKVKIQVFFYDTVDDKDIKLTDADVTYEWLTPNHDWADVKPEVL
                     AVNYVRPKNKVLPSEAALSAAAASVNPGKKTRPNKPGSPEGQRKYLGYVVRVYYQDQL
                     QAVRADPNSLLKKFPPPSTASP"
     gene            6221..6928
                     /locus_tag="VFO30_03540"
     CDS             6221..6928
                     /locus_tag="VFO30_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9418390.1"
                     /translation="MQLVVVHRDAELGGQLVQMVKDYTPHDCDLVASETAAMQWARDH
                     SHCAFLLTQLDSMIDGLSLGASFSEMFPGVQTAFLPGYPRSEQRLELEETKVFPEPIN
                     GEMLLALIDRIANASPSAPDLFHVVDVLQMCCLSGLDGALQAVTANQSGIVYLRGGEI
                     VHAERERVRGKPALLEIIGWRFVEFGYDRTICPSMETIACPWQTALVEAVAVSKEQSA
                     ASRLPQTLWAKLPSRAS"
     gene            6932..7837
                     /locus_tag="VFO30_03545"
     CDS             6932..7837
                     /locus_tag="VFO30_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418391.1"
                     /translation="MSPSAWFSRRIKFAFASACLLCAAAQARGQLQVELNLKRLQYIA
                     YEPILATVGITNLAGRDVDLHDGDGQAWFGFEVSGKEGEPIAPVTPNYTQPPLKIPAG
                     KKVTQRINLTPIYSVHDFGTYHLRAHIYFADLNKFFYSQAKVFEVTDARPIWQRTVGV
                     AEGTPASGKTRTYSLLSNRFPDHTALYVRVEDKDSGAVYATYSLGRSIEFGEPQAEVD
                     RSNQLHVLHCAAPRVWQYSRVGLDGQLLAHSSFMETKTRPRLVHLDDGKVAVRGGMIE
                     TPATGTARDSLPKLSDRPAEMPKEN"
     gene            7838..8716
                     /locus_tag="VFO30_03550"
     CDS             7838..8716
                     /locus_tag="VFO30_03550"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9418392.1"
                     /translation="MLQHGWRVRFVGAALCLATSTFALSSSAANSSSASTVVIDAGHG
                     GFDRGGIPGQRVAEKMMTLDVAQRLKSILAGNGYRVVMTRDEDVFVPLGTRVAIANSY
                     PNGIFVCIHFNSARRIGANGIETYFYSRQSLPLASAIHYYVAGGAPSMNRGVRRRSYY
                     VLRYSAVPSVLVECGFLTNPTEAQYAQTVSYRQQLAEEIARGIRASSLVATSSASGRL
                     ATAEAVPLQPFIDQTKVRDPEISASKRSKRGSRSRAKHKKASQVSRLENGAMTNKSDD
                     SNSTRSKETSRQHLED"
     gene            8747..>9246
                     /locus_tag="VFO30_03555"
     CDS             8747..>9246
                     /locus_tag="VFO30_03555"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HET9418393.1"
                     /translation="MRAAEKAAFGRGVGVETLMDCAGAGVARAVRKFFPQPGKCLVYA
                     GKGHNGGDALVAAEHLARSGWNIDIHLVFPESDCSELTRKKLNSAREAAPGGGGSLAP
                     TPSLVVLDGLLGLGASRLLRQPVRAAAHEINRHRRDQNGFVFAVDVPTGLDSDSGEVD
                     PEDCVTA"
BASE COUNT         1975 a         2732 c         2668 g         1871 t
ORIGIN      
        1 gagatcaccg gcgcgatcaa ctgtctatat gacatcacag agcgcaagta cgcggaacaa
       61 cagcaggaag cactctatca gttcgtccag cgccagtcgc tcgccgcttc cgtgcgcgac
      121 atttacgacg cggcgcttga agcgattgtc ttgaccatgc gctgcgatcg cgcttccatt
      181 ctcctgttcg atcatcacga cgtgatgcga ttcgtcgcct ggcgaggctt gtccaaagcc
      241 taccgtaaag cagttgaggg acattcgcct tggaaacgcg acgtagaaga tccgcacccg
      301 gtttgcatcg aacatgtcga tcttgccagg cttcctaagc cgttgaagac gactatcaaa
      361 gccgaaggaa tcaccgcggc ggcgttcatt ccactggtcg cgaacgggaa attgttcggc
      421 aaattcatgg cttattacga tcagccgcac gttttcactg agaaggagat gactctcgcg
      481 gtgagcatcg cgcggcagct caccctcggg ctggaacgca aacgcgcgga ggaagaattg
      541 cgcaagtcaa agctgttgtt ggagcagcgg gtgcaacagc ggacggaagc attgcgcgcc
      601 gcgaaccgcg aactgcaacg tgaaatcgag cgccgcaaag gactcgaagg cgagatcctc
      661 gaggtgagcg accgcgagca gcagcggctt gggcaggagt tacacgatgg tctgtgtcag
      721 caacttgcgg cgatcggttt catggcgcgc gcaaccgcgt tgcgtgtcaa agaccatcgc
      781 gtcgttcagg tcgaggacct cgagaagatt gcgcagttgg tcaacgcctc ggtcagtgac
      841 gcgcgcaata tcgcgcgtga cctgcacaag gaggaagtgg acgctgccgg gttcgtcgag
      901 gcgctgcgca gcctgaccca acgtgagatg tggaaaacgc gctgccgctt tgagttgaaa
      961 accgacgtgc acctcgaaga cgacaggacg gcgtcgcagc tctaccggat tttgcgcgag
     1021 gcgctgatca atgcgcacaa gcatgcccaa gccagtcaga ttgtcctcga agtgcgccaa
     1081 atgaaaacca atctcgtctt cagcgttacg gacaatggcg ttggatttag cagaaaaacg
     1141 aaaaccggtc gcggcctggg ttttcacatc atgcagtacc gggcgcgctc agttggtgcg
     1201 cgcttgaaac tcgaatcgcc agccaaacgc ggcgcccggg tcgtctgtta tttgccgcac
     1261 ccaaatgacg ctcgctaaat tccgctgcac caccgcaacc acgaatgaaa ctcgccgcat
     1321 caaacttgga agctccagaa gccgctgcgc cggcaaagaa aaaacgcatc atcatcgtcg
     1381 atgatcatcc cctgttccga aaagggctcg agcaattgat caactccgat gggggactca
     1441 ccgtctgcgg agaagcgggc gacgcgagcg cagccatgac cgtgatccgc gaaaagcgac
     1501 ccgatcttgt aattgtcgat cttagtctgc ccggcgcgaa cgggattgag ttgatcaaga
     1561 acatccgggc cgagtttcac aaggtgccgg tgctaactct ctcgatgcac gacgagtccc
     1621 tttacgcgtt gcgtgctctc cgggccgggt cgcagggtta cgtcatgaaa caggaagcgc
     1681 tcgagaatgt gattaccgcg attcgggaag tgttcgcggg ccggccgtac ctcagccccg
     1741 cgatgtcagc gacggtcatc gcgaagtttg cgcaacgctc gagcggtgac gtgagcgcca
     1801 accttgtcga caggttgagc gaccgtgaac tggagattct cgagttgatc ggcaaggggc
     1861 gcgacgtgcg cgagatcgcg aagatgttgc atttgagtcc gaaaacggtc gagacgcacc
     1921 gcgcccacat taaagaaaaa ctgagtctcg cgaacgcgcg gcaggtcgcg cgcttcgccg
     1981 tgcagtgggt cgggcaaaaa ggcgtttaac gcaagaattc ggctcccata cctgcggtcg
     2041 tatggtcacc tcaaggcggc cacgccaagc gcggcacaca acgtacccag cgcgacgatc
     2101 gctacgattg cgagattgct cgtttcgctc ggactgagat cggtttagct cagttcagta
     2161 gcaacctccg gcgaaccgcc aacgcattca tctgcgatga actagtgaga ggagacgaga
     2221 tccatggcta aaattgtcaa agtcatcgaa gtgctgtcgg aatcgccgac gagttgggag
     2281 gaggcaacgc agaacgttgt gaccgaagcg gccaaaacgc tgcgcaacat tcgttccgtt
     2341 tacatcaagg agctcaccgc tgcggtcgag aacgacaaag tcaccagcta ccgagtaaac
     2401 gcgaaggtca cgttcgagat ggagcgctga gcgcgctcaa acgcacctgt agccacggcc
     2461 ctgcggccgt ctaacgcgcc acaggcgcgt ggctacaagc atttacccgc gatcgcgcag
     2521 cttgttctcg ccgctttcgc gcgcgcaatg tgcgcagcag aagattttgc cgccggcttc
     2581 cacgccgtga ccgacgattt tgcagccgca gtgctcgcac tccggcgcca tcaccgcgat
     2641 cgcgcactcg aagctgtcga atatatgcgt cttgccgtcc tttctcagct caaacgtttt
     2701 atcgtagtcg tttccgcaga gttcacattt agccatcgat gttctggtta aaggccttcc
     2761 tcctctaaaa agtaacgtga cggaccattg cgacgcgtgc gtttttcgtg actcagctcc
     2821 ggcggtcacc ggccggtgga agacgtctcg tagcgcgatc cacctacttc ttcggcttga
     2881 gcagtggaaa aagaatgaca tcgcggatgg attccgcgcc tgtcagtaac attgtcaatc
     2941 ggtcgatgcc tatgccgacg ccaccggccg gcggcatccc gtgctcgagc gcgagcagaa
     3001 aatcttcgtc gatcttttgg gtctcttcgc ctgcttgctc cagtaagcgt cggcgctgca
     3061 cctgcggatc attcaactcg ctgtaacccg gcgcgatctc cgcaccgtta atgatcaatt
     3121 cgaacacatc gaccagcgat tggtcctcgc ggttctgccg agctaagggc accagctctt
     3181 tcggacaatg ggtgacaaag agcggatcga acgttttttc ctcgaccagc ttttcgaaaa
     3241 cgtgctgggt cacttcgtaa tcgacgagct gcggtagaac ctccagcttg aactcacgcg
     3301 tcgcgcgggc gcggcgttcg tcgggagaaa ggtcgaacca atcctcgccc gcaacgtcgc
     3361 ccacaagatc gacatatcgc gcccggcgcc acgggcgttg caagttgatt gtgcgaacaa
     3421 tccttccatc cgcgtcacga tgctcaagtt gcaccgagcc gcagactttc tcggccacgt
     3481 ggcaaatcag ttcctccacc agattggcga ttttctcgaa gtccgcgtac gcccaataga
     3541 tttcgagcat cgtgaactcg ggattgtgtt tccgggaaat gccttcgttg cgaaagttcc
     3601 ggttcaattc aaaaactttg ttgaacccac cgacgagcaa ctgcttgaga aacagctctg
     3661 gagcaatgcg caggtaaaga tcgagcccaa gcgctttgtg atgcgtttgg aaaggctggg
     3721 ccgccgcccc gcctgccacg gtctggagaa tcggtgtttc gacctcgaga aatccgcggt
     3781 tctcaagaaa gtgccgcgtc tcgcgcacca tcgcgatgcg ctttaaaaaa atctcacgcg
     3841 agcgctcgtt cgtgagaaga tcgaggtagc gctggcgata acgcgcttcg atgtcctgga
     3901 gcccgtgcca tttttccggt agtgggcgca gtgacttcgc gaggaccgcc aaccgcccaa
     3961 gcctgattgt cggctcaccg gttttggtga cgaaacaggt accctcgacg ccaatgaaat
     4021 cgccgacatc gatgagcggg aaaagctcga cgatttccgg cccaacttcc ttagtctgaa
     4081 tataaatctg gatgcggccg gtcgcatcgc gaagatcgac aaagtggctt tggcccatgt
     4141 cgcgatgcgc cgtgacgcgc ccggccacac gcaccgtttc accctctgta aatttcgccc
     4201 ggatttcggc gatatcgccg ctgatttcga aagcgttgcc gaacggctcg atcccacgcg
     4261 cgcgcaaagc gtcaagtttt tggcgtcgca acacaatcag ctcgttctct tcgtcgatca
     4321 tcggtctgcg aatctaaagc agggtgggca aataaatagc ggcaaggtga atcgtgctct
     4381 ccgagtgcga tgttagttca ggcagcggag ccgcggtaat tagcatcggc cgcggagcgg
     4441 ccgatccacc caatattcgt ttgccaatga aagaccgaca atgagatatt cgcgcgctgg
     4501 atcggcttct ccgaagacga tgctaaacat ggcggcgcgg ccgcattatt taaacaacgc
     4561 gttcggagaa cgcgttccac cttatgcctc tcgatctacc tgagacaact tctaggctcg
     4621 gtggaccgct ggtgcggcaa ttttcggttt ttttgcctaa caaagtcgga gcgttgctcg
     4681 acatcctgaa gttgctgaac acccataaca cgcacgtcgt ggcgatgagt gtgtcggagt
     4741 cgaccgactc ggccatcgcg cgtgtcgtcg tgagcgatcc ggagcaagtc gaagagttgt
     4801 ttcgcgcaca tgacgtcgcg tttggtgttt ccgaagttct cgtcgttgag atgcgcgaag
     4861 tggccacgca actggcgagc ctgttcgccg ctttgctcaa agcggaggtg aacgtgcatt
     4921 ttagttatgc atttttgacc cggccgcgcg gccttgctgc actcgccctt cacgtcgatg
     4981 acgttgaatg cgcctcctcg gtcatcaccg gtgacggttt caagctcctg agtcaggacg
     5041 acatttcgcg ctgatggcgc tcgccgggtt tagtccacac gttgtcgcgt caatctcgtc
     5101 agtgccggcg gttgacaatt tttcgaaacc gcgtagtgcc gtcgctcgac atgcgaacgt
     5161 ttagcatttc gtcgcccgtc acggcggacc gcagtttcat agttgctctc ggtttcgtcg
     5221 cgtttttcgc cgcggccgag atttgtgctg tggcatatca ttacgtcgga ccggtgcgaa
     5281 tcggggccgc gaccgcaccg gccagcgttg ctgtgactcg aactccagcg ccggcgccag
     5341 cggccactgc tgcgcctgca gccgcgtcca gcgcagcgcc actttccccg agcgatgggg
     5401 caataaaagc ggcaatcgcg ctgcgcgacc ggggcgacac cgccaacgca ctcgcgaaat
     5461 tgcaaacggc gtcagaacaa gatccgagca acccgaacat tctcgaggaa ctcgcccgga
     5521 cttacgaagc ggtgcaaaat gtcgaacttg caaacgtgac ctggcgcaaa atccaggagc
     5581 tcgggcctgc ggcggggtcg tcgtttgcgt tggccgacca gcggctgaaa gccggtttgg
     5641 ccacaccagc ggcgagcggc gccggtttac ccggccctgc tcggcttgac actgcgagct
     5701 cagagccagc cgcaccgcca tctacggata catccgttct cacgatcagc gatgtgcaga
     5761 taactgaaac gccggatcca gatgcggaca caaacctgac ggtgcgcgtc ggaattcaaa
     5821 agcgcacgaa cacggtaatc gaccacacca aggtgaaaat tcaggtgttc ttttatgaca
     5881 cggttgacga caaggatatc aagctcaccg acgccgacgt tacatacgag tggctgaccc
     5941 caaatcatga ttgggcggac gtcaaacccg aagtgctggc ggtgaattat gtgcgcccga
     6001 aaaacaaagt gctgccgtcc gaagcggcgc tttctgccgc tgcggcgtcg gttaatccgg
     6061 ggaaaaagac acggccgaac aaaccgggta gtcccgaagg tcagcgcaaa tatctcgggt
     6121 acgtcgtgcg cgtctattat caggaccagc ttcaggccgt gcgcgccgac ccgaacagcc
     6181 tgctcaagaa attcccgccg ccttcgactg cgtcgccctg atgcaattgg tcgttgtgca
     6241 ccgcgacgcc gagttgggcg gccagttggt gcagatggtg aaggattata cgccgcacga
     6301 ctgcgatttg gtcgcaagcg agaccgccgc catgcaatgg gcgcgcgacc actcgcattg
     6361 cgcgttcctt ctaacgcagc tcgacagcat gattgacgga ctcagtttgg gggcctcctt
     6421 tagcgaaatg ttccccggtg tgcaaacggc gtttctcccc ggctatccgc ggtcggagca
     6481 acggcttgaa ctggaggaga caaaggtttt tcccgaaccg atcaacggcg aaatgttgct
     6541 cgcgctaatt gatcgcatcg caaatgccag ccccagcgcg cccgatcttt ttcacgttgt
     6601 cgatgttttg caaatgtgtt gcctgagcgg actggacggt gcactgcaag cagtgacggc
     6661 caaccagagc gggatcgtct acttgcgagg cggcgagatt gtccacgccg agcgcgagcg
     6721 cgttcgcggt aagccagctt tgctcgagat tatcggctgg cgctttgtcg aattcggcta
     6781 tgaccgcaca atttgccctt caatggaaac aatcgcttgc ccttggcaga cggcgctcgt
     6841 cgaagctgta gccgtatcga aggagcagag cgcggccagc cggctccctc agactctctg
     6901 ggcaaaactg cccagccgcg ctagctgatt tatgtcgcca tccgcgtggt tctcccggcg
     6961 gattaaattc gccttcgcat ccgcgtgcct cctttgcgca gcagcgcagg cgcggggaca
     7021 actgcaagtt gagctcaatt taaagcgact gcagtacatc gcctacgaac cgatcctggc
     7081 gacggttggc atcactaacc tcgccggccg cgatgtcgat cttcacgatg gcgatgggca
     7141 ggcgtggttc ggttttgaag tcagtggtaa agagggcgag ccgattgcgc cggtcacgcc
     7201 gaactacacc cagccgccgc tgaaaattcc agccggtaaa aaggtcacgc aacggatcaa
     7261 cctcacgccg atctattcgg tgcacgattt cggcacctac cacctgcgtg ctcacattta
     7321 tttcgccgat ctgaacaaat tcttttactc gcaggcaaag gtcttcgaag tgactgatgc
     7381 gcgcccgatc tggcaacgga ccgtaggcgt cgcggagggt acgcccgcgt ccggcaaaac
     7441 acgcacctat tcgctcctct cgaaccgctt tcctgatcac actgcgcttt acgtgcgggt
     7501 cgaagacaaa gacagcggcg cggtctacgc gacttattcg ttaggccgat cgatcgaatt
     7561 cggcgaaccg caggccgaag tcgatcgctc aaatcaactc cacgtcctgc attgcgcggc
     7621 gccgcgcgtc tggcagtact cgcgcgttgg actcgacggg cagctcctcg cccattcgtc
     7681 attcatggaa acaaagacgc gaccgcgcct cgttcatctt gacgatggca aggtagcggt
     7741 gcgcggcgga atgattgaga cgccggcaac gggaacggca cgcgattcgc tcccgaaatt
     7801 gtccgatcgc ccggcggaga tgccgaagga gaactaaatg ctgcaacacg gttggcgcgt
     7861 caggtttgtc ggcgccgcgc tctgtcttgc caccagcacc ttcgcactct cctcttcagc
     7921 cgcgaacagc tcgtccgcat cgactgtcgt gatcgatgcg ggccacggcg gttttgatcg
     7981 cggcggcatt cccgggcaac gcgtcgcaga gaaaatgatg acattggacg tcgcccagcg
     8041 tctcaagtcg attctcgccg gcaatggata tcgtgtggta atgacgcgcg atgaggatgt
     8101 cttcgtcccg cttgggacgc gcgtcgccat tgccaactct tacccaaacg ggattttcgt
     8161 ttgcatccat tttaattctg cgcgtcgcat aggcgctaac ggtatcgaaa cttacttcta
     8221 cagccggcaa agcttgccgc tagcctcggc aattcactat tacgtcgcgg gtggagcccc
     8281 atcgatgaat cgcggcgtcc ggcgccgtag ctattatgtg ctccgctata gcgcagttcc
     8341 ctctgtcctt gtcgaatgcg gtttcctgac caaccccaca gaagcgcaat acgcgcagac
     8401 cgtctcttat cgccagcaac tggccgagga aatcgcgcgc ggcattcgcg cgtcctcgtt
     8461 agtagcgacc agctcggctt caggacgcct cgctaccgcc gaagctgtcc cgctgcagcc
     8521 gttcatcgat caaacgaagg tgcgtgatcc cgaaatctcg gcgtcgaaac gctcgaaacg
     8581 cggctcacga tcacgcgcga agcacaaaaa ggcatcgcag gtctctcgtc tcgaaaacgg
     8641 agcaatgaca aacaaatcgg acgacagcaa tagcacccga agtaaggaga catcgcgcca
     8701 gcacctggag gactgattgg actcgccggt cttaagctcg gcgcaaatgc gggccgcgga
     8761 aaaagcggcc ttcgggcgcg gtgtcggtgt tgaaacgctg atggactgcg cgggcgcggg
     8821 cgttgcacgc gccgtgcgca agttttttcc gcagccgggt aagtgcctgg tctacgccgg
     8881 gaaaggtcac aatggaggcg atgcattggt cgccgcagaa catcttgcgc gcagcggctg
     8941 gaacatcgat atccacttgg tctttccgga aagcgactgc agcgagctga cacggaaaaa
     9001 actcaactcg gctagagaag ccgctccggg cggcggggga tcgctcgcac caactccatc
     9061 tttggtggtg ctcgacggct tactcggcct cggcgccagc agattgttgc gccagccagt
     9121 gcgcgctgcc gcgcacgaga tcaatcggca tcgccgcgat caaaacggat tcgtttttgc
     9181 cgtggatgtt cccaccggac tcgacagcga ttccggcgaa gtcgatccag aagattgcgt
     9241 cactgc
//
LOCUS       DASTDG010000114         9954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_570898, whole genome shotgun sequence.
ACCESSION   DASTDG010000114 DASTDG010000000
VERSION     DASTDG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570898"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VFO30_03560"
     CDS             complement(<1..1408)
                     /locus_tag="VFO30_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9418394.1"
                     /translation="MYNANEKVDPVNVAEEVSRSFLDYSMSVIISRALPDARDGLKPS
                     QRRILYAMHDLSLFPGRQHRKCAKICGDTSGNYHPHGEAVIYPTLVHMAQPWAMREPL
                     VDGQGNFGSVEGDPPAAMRYTEARMTHLGAALMVDMDKDTVDFVPNYDETRTEPTVFP
                     AAFPNLLVNGGTGIAVGMATNIPPHNLREVIDGVCAQIDNPDIVLDELMEHVRGPDFP
                     TGCVICGVGGIRQYFETGRGSVKVRGRAGVEELKGGKEQIVITEIPYNVNRATLVERV
                     AELVNEKVLSEITAVRDESDENTRVVIELKRDANPKVVINNLYRHTAMESSFAVILLA
                     IDHGRPKLLSLKEANAAYIEHRREVVLRRTRFELRKAEERAETLEGYLIALANLDEFI
                     RIIRGSANRDEARVKLLAFEFTKRQVEQIGILIRNRARLTDGRYAFSEHQANQILDLR
                     LYQLTGLERDKIDNEYKEL"
     gene            complement(1401..2201)
                     /locus_tag="VFO30_03565"
     CDS             complement(1401..2201)
                     /locus_tag="VFO30_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HET9418395.1"
                     /translation="MSSFARLLPHWRRCYMLCAVARSGSNLLADGLVQVRRAGRPGQY
                     FLPQNEVRTALDHGIDPNASFASYIRALVSVAASANGVFGFKIMGWYLEKFVTRMRET
                     GAFGGAELPDVETLRSAFPRLQFVQILRRNKVRQAISKARALQTGLWKIQEGKTPVRQ
                     AQFDPQLLARCISDIRREEEIWADFFARSGVTPFRVEYEELCRDYEPTIRGVLDFLKV
                     RLPRRIKISSPVTVSQTDETSREWEERFLAMSSRAPQPQRQVLTTAHV"
     gene            complement(2221..3060)
                     /locus_tag="VFO30_03570"
     CDS             complement(2221..3060)
                     /locus_tag="VFO30_03570"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HET9418396.1"
                     /translation="MMRALLHKLRYPQRCYVICAVPRCGSNLLSDGLTAMQKAGRPKQ
                     YFLPEFEERYGEKYGLDPAKDYAGYVRGIVRATSARNNVFGFKLMGSYLQKFLVQLRA
                     TGAFGGAGTSDLELLNEAFPRLRFVRIFRGDKLRQAISKARAMQTGVWKIAGGKQTAG
                     HARFDAQLITRCLRDGERQEAIWSRFFTAGRLSPFTVEYETLCRAYSDTLQQVLDFLG
                     ISSVPNTATGAPTTVRQADDVSEQWYMRYRDSLANGGAWSVVTFVLQFWLDAIDLTAL
                     LPC"
     gene            complement(3060..5651)
                     /gene="gyrB"
                     /locus_tag="VFO30_03575"
     CDS             complement(3060..5651)
                     /gene="gyrB"
                     /locus_tag="VFO30_03575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9418397.1"
                     /translation="MPSRAVALVDSDDANSDEQNRHREFSGVSKLQKYDAAQIDKLEG
                     LEAVRKRPGMYIGDPDERGLHHMVYEVVDNSIDEHLAGFCDQIEVTVHVDGSVSVRDN
                     GRGIPVDIHPKWKMPAVELVLTNLHAGGKFGQGAYKYSGGLHGVGAKCTNALSEWFKV
                     EVSRDGKVYHMAFAKGKTTQKLEIIGEVKNKKTTGTLITFLPDPIIFTITTEFKFERL
                     ATRLRELAFLNPGLEINLTDERDESPKKETFLYKHGIEEFVKQLGENKQVLHPKPIVI
                     ARQREEVFVDVVLQYNDSYNDQILPFANSIANPDGGTHLTGFRTALTKAVNQYAKQNS
                     LLKDKDPAISGEDVREGLIAVLSVKLPNPRFESQTKVKLVNTEIDGIVNSAVYEGLMT
                     YFDKNPPVARRIFDKVLTAARAREAARKARETIRKGALTGGGLPGKLADCSERDPELT
                     ELYIVEGDSAGGSAKQGRDRRYQAILPIRGKLINVEKAREDQFLKNTEIQSMITAIGT
                     GIGKPKEDGNGKDEGRFDMSKLRYGRIIIMTDADVDGSHIRTLLLTFFYRQMTELVRA
                     GRIYIAQPPLYQIKRKKREEYVDDDVQLNKILIALGAEDVRLKNLADNKEITAAQLKD
                     ILESLEKLAKLSEAVRRHGGDFESYLAKRNPRTGKLPAYVVKVRAGNDETVHYFADEK
                     AVRKFHQENLDLNLFDTELGQELLPLGDVPATRPNGRRRGKLVELHESAAIEKIIAEL
                     ARKGLQVSHYAASDRPIFELIEGEGEKAISHPLFSIPEILHKILEIGRRGVQIKRFKG
                     LGEMNAKELFETTMNPEKRKLLKVDLNDDNAVDADKMFTILMGDVVEPRRQFIEDNAL
                     NVRNLDV"
     gene            5842..6477
                     /locus_tag="VFO30_03580"
     CDS             5842..6477
                     /locus_tag="VFO30_03580"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET9418398.1"
                     /translation="MRFQTLLEARWIFAALAFLTVVSSVIFPPLAFGFLLLILYTFFF
                     FRDPDRAAPEDPGAIVAAADGTVAEIAEVYELQILKTKTQRVAIFLSIFDVHTNRAPI
                     EGRIFYRQHREGLCLDARSTDCSAKNEAMTWAFENPQTTIVVRQLTGAIARRIVAWSD
                     VGDYVEKGERFGMIRFGSRTEVYLPLTATVLVKVGDHVSAGSTIIARLPEP"
     gene            6474..7298
                     /gene="pssA"
                     /locus_tag="VFO30_03585"
     CDS             6474..7298
                     /gene="pssA"
                     /locus_tag="VFO30_03585"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET9418399.1"
                     /translation="MSEHNSKIYLLPNLMTAGNLFCGFTATLKILEGALLQGSNPEAA
                     ADLFHAAVWFILGAFVFDFLDGRLARLGGNETPFGREFDSLADIVSFGLAPALMVYRI
                     VLQEFPRACWIIAFIYLACGALRLARFNTAAINRTGGDGTTFTGFPIPAAAGLIASIT
                     LFLLWLAEGEHRLGRWKFVLPPLLLFVSLMMFSRFQYPSFKAINWKTQKSIPRFLAII
                     LILILTVLNPEWMPAVLFLAYLLYGFLRPWLSHEWRREIEEEIGEEPSEQAAEQMP"
     gene            7394..9133
                     /locus_tag="VFO30_03590"
     CDS             7394..9133
                     /locus_tag="VFO30_03590"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET9418400.1"
                     /translation="MRAPAQPPAPRNPDVSNKALITKTRPRIDATRSTSADIALMRKM
                     ADDYYAWRNENYPVASSDRGLHTWDDRLTDYSPAQVAERARHVRSLLDKVRALKIDKW
                     PKDDRVDAILFRAQLESVEFGDRVLKFERTNPQVYVAECVNGIFSLLKKDYDTPRKRA
                     LAATARLKAMPPMLRQGLSNLQKPVKLYAQLAIQSARAMDPLLNQSLMALDVDLPPNE
                     HDELVKSRDAALAAAHGFADELEKRLNKMVDFAPMGETNYNYYLKHVLLLPLSAAEVE
                     MIGRAELARYRALEAMLPDPKLADPDPKRSGNIPPDQEAFLMAYESRETEMINFLKEH
                     NLVTLPDYLGPFQIRQLPEAFKPTSPGGFMNPPGVYDKDPTGFYFLPSYNPESKNFYI
                     RAAIEDPRPILGHEGIPGHFLQLSIANHLTDEIRRQHEDGVFVEGWALYGEEMLMRTG
                     LYPNNSPAQGQILRLSRYRAARIGVDVNLHTGRWSFEQAVTYFMEAGGLDREAAEGEA
                     AGAASNPHQKISYIIGKWQIMNLLGRYKDRQGDNFRLGQFHDDLIKNGSLPVSVIEWV
                     LLDDPASVEKALR"
     gene            9196..9513
                     /locus_tag="VFO30_03595"
     CDS             9196..9513
                     /locus_tag="VFO30_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418401.1"
                     /translation="MIVDGCVNHPRFAAPPRFLELYSEARVATLHFPRGSYAFDSEDS
                     RGYYYRAPSGVLEHTAAGPRRREGGIFVSKRNRDRLRGYVIMPYGLTHVGNLSRTEHE
                     FRD"
     gene            9749..>9954
                     /locus_tag="VFO30_03600"
     CDS             9749..>9954
                     /locus_tag="VFO30_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418402.1"
                     /translation="MLPYSNALDRAFHSFPETTFSFQITSPTEMFGGMVLKSWGVRKS
                     PTAIAEAGADAVTSGAHDKRRHGLR"
BASE COUNT         2013 a         2946 c         2778 g         2217 t
ORIGIN      
        1 gcagctcctt gtactcgttg tcgatcttat cgcgctcgag accggtcaat tgatacagcc
       61 gaagatcgag aatctggttg gcctgatgtt cgctgaacgc gtagcgccca tccgtcaggc
      121 gcgcccgatt gcggatgaga atgccgattt gttcgacctg gcgtttagta aattcgaacg
      181 cgagcagttt cacccgcgct tcatcgcgat tggctgaacc gcggatgatg cggatgaatt
      241 cgtccaggtt ggcgagcgca atgagataac cttcgagcgt ctccgcacgc tcttctgcct
      301 tgcgcaattc gaagcgtgtc cggcgcagca cgacctcgcg gcgatgctcg atataagcgg
      361 cgttcgcttc cttgagcgag agcagcttcg gccggccatg gtcgatcgcg agcaggatga
      421 cggcgaagga gctttccatc gcggtatgcc tgtaaaggtt gttgatgacg acctttgggt
      481 tagcgtcgcg cttcagctcg atgaccacgc gggtgttttc gtccgattcg tcacgcactg
      541 ccgttatctc gcttagcact ttctcgttca cgagttccgc aacccgctcg acaagcgtgg
      601 cgcggttcac gttgtaagga atttcggtga tgacgatctg ttccttaccg cccttaagct
      661 cctcgacgcc ggcgcgaccg cgcactttca cgctgccgcg accagtctca aaatattgcc
      721 ggattccacc gacaccgcag ataacgcaac cggtcgggaa atccggtcct ctcacgtgct
      781 ccatcagctc gtccaggacg atgtcgggat tgtcgatctg cgcgcaaaca ccgtcgatga
      841 cttccctcaa attgtgcggc ggaatgttgg tcgccatccc cacggcgatc ccggtaccgc
      901 cgttgacgag caggttcggg aacgccgccg ggaacaccgt cggctcggtg cgcgtctcgt
      961 cgtaattcgg gacgaaatcg acggtgtcct tgtccatgtc gaccatgagc gccgcgccga
     1021 gatgggtcat gcgcgcctcg gtgtaacgca tcgccgccgg cggatcgcct tcgaccgaac
     1081 caaagttccc ttgcccgtcg acgagcggct cgcgcatcgc ccacggctgc gccatgtgca
     1141 cgagcgtcgg ataaatcacc gcttcgccat gtgggtgata attaccgctc gtgtcgccgc
     1201 agatcttcgc gcatttccgg tgctgccgtc ccgggaaaag ggacaaatcg tgcatggcgt
     1261 agagaattct gcgctgcgat ggcttgaggc cgtcacgcgc gtccggcagc gcgcgcgaga
     1321 tgatgaccga catcgagtag tcgaggaacg acctcgacac ctcttcggcg acgttcaccg
     1381 gatcgacctt ttcgttggcg ttatacatga gctgtcgtga gtacttgccg ttgcggctgc
     1441 ggcgcccgcg aactcatcgc caaaaaccgt tcttcccatt cccgcgatgt ttcgtctgtc
     1501 tggctgacgg tgaccggtga gctgatttta attcgccgcg gtaacctgac tttcaaaaaa
     1561 tcgagaacac cgcgaatcgt cggctcgtag tcgcggcaga gttcttcgta ctcgactcga
     1621 aacggcgtca cgccactgcg cgcgaaaaaa tctgcccata tttcttcctc gcggcggatg
     1681 tcactgatgc agcgcgcgag caattgcgga tcaaactgcg cttgccgaac cggcgttttg
     1741 ccttcctgga ttttccacag cccggtctgc aacgcgcgcg ctttggagat cgcctgacgc
     1801 actttatttc ggcgcaagat ctggacgaat tggagccgcg ggaaagcgct gcgcaacgtt
     1861 tccacgtcgg gcagttccgc acccccaaac gcgccggttt cacgcatccg cgtgacaaat
     1921 ttttccagat accaacccat gatcttaaaa ccgaagacgc cgtttgcgct cgccgctacg
     1981 gaaacgagcg cgcgaatgta agacgcgaag ctcgcgttag ggtcgatgcc atgatcgagc
     2041 gcggtgcgca cctcgttctg cggcaaaaaa tattgtcccg ggcgaccggc gcgccgtacc
     2101 tgcacgagtc catcggccag aagattgctg ccggaacgcg caaccgcaca gagcatgtaa
     2161 caacgccgcc agtgcggcaa aagacgcgca aaactagaca tcactgtaag atcgacatca
     2221 tcaacagggc aggagcgcag tcagatcgat cgcgtccagc cagaactgca acacaaatgt
     2281 aacaacgctc cacgcgccgc cgttggctaa actatcgcga taacgcatat accattgctc
     2341 cgaaacgtcg tccgcctgcc ggactgttgt tggcgcgccg gtcgcagtgt ttggaactga
     2401 cgaaatacca agaaaatcca acacctgttg caaagtatcg ctgtaggcac ggcacaatgt
     2461 ttcgtactcg accgtgaacg gcgataaccg gccggcagtg aagaaacgag accagatcgc
     2521 ttcttgacgc tccccatcgc gcaaacaccg cgtgatcaat tgcgcatcga aacgcgcgtg
     2581 ccccgcggtc tgctttcctc ctgcgatttt ccagaccccg gtttgcattg cgcgggcttt
     2641 cgagatcgcc tggcgcagct tgtcaccgcg gaaaatgcgg acgaaccgca gccggggaaa
     2701 cgcttcattg agcaactcaa ggtcactggt tcctgcaccg ccaaatgcgc cggtcgcgcg
     2761 cagttgcact aaaaacttct gcaaatacga gcccatcaat ttgaatccga agacgttgtt
     2821 gcgagccgat gtcgcccgca cgatcccgcg cacgtaaccc gcgtaatcct tcgctggatc
     2881 caacccgtac ttctcgccat acctttcctc gaactcgggc agaaagtatt gtttcggccg
     2941 ccccgctttt tgcatagccg ttaaaccatc actcaggaga ttgctgccac agcgcggcac
     3001 cgcacaaatc acataacatc gctgcgggta tcgcagcttg tgcagcaacg cacgcatcat
     3061 cagacgtcca ggttgcggac gttgagcgca ttgtcctcga tgaattgccg ccgcggctcg
     3121 accacgtcgc ccatcaaaat cgtgaacatc ttgtcggcat cgacagcatt gtcgtcgttc
     3181 aggtcgactt tcaagagctt gcgcttctcc gggttcatcg tcgtctcgaa caattctttc
     3241 gcgttcattt cgccgagacc tttgaagcgt ttgatctgca caccacgccg cccgatctcc
     3301 aaaatcttat ggagaatctc gggaatcgag aaaagcggat gcgagatggc tttctcccct
     3361 tcgccttcaa tcagctcgaa gatcgggcga tcgctcgccg cgtaatgact gacctgaaga
     3421 cctttgcgtg cgagctcggc aatgatcttc tcgattgccg ccgactcatg cagttcgacc
     3481 agtttgcccc ggcggcggcc gttggggcgc gtggctggga catcgccgag cggcaaaagt
     3541 tcctgtccga gctcggtgtc gaacaaattc aaatccagat tctcctgatg aaattttcgt
     3601 actgccttct cgtcggcgaa gtagtggacc gtttcgtcgt tgcccgcgcg cacctttacc
     3661 acataagccg gcagcttgcc cgttctcgga tttcgcttcg cgagatagct ttcgaaatcg
     3721 ccgccgtgac gccgcaccgc ttcgctcaac ttcgcgagct tctcaagcga ctcgagaatg
     3781 tccttcaact gcgcggcagt gatctcctta ttgtcggcga gattcttcag ccgcacatcc
     3841 tcagcaccga gcgcaatcag gattttgttt aactgaacat cgtcatccac atactcctcg
     3901 cgtttcttgc gcttgatctg ataaagcggc ggttgcgcga tgtagatgcg gccagcgcgg
     3961 acgagctcgg tcatttgccg atagaaaaaa gtgagcaaca gcgtgcggat gtgcgaaccg
     4021 tcaacgtcgg cgtcggtcat gatgatgatg cgaccataac gcaacttcga catgtcgaag
     4081 cgaccttcat ccttgccgtt gccgtcctct ttcggcttgc cgatcccggt gccgatcgca
     4141 gtgatcatcg actggatctc ggtgttcttg aggaactgat cctcgcgcgc tttctcgacg
     4201 ttgatcaact ttccgcggat cgggagaatg gcctggtagc ggcggtcgcg tccctgcttc
     4261 gccgagccgc cggcggaatc gccttcgacg atgtagagtt ccgtcagctc cggatcgcgc
     4321 tcggaacaat cggctagttt gcccggcaac ccgccgccag tcagcgcgcc tttgcgaatc
     4381 gtctcccgcg ctttgcgcgc cgcttcacgg gcgcgggctg cagtcaacac cttgtcgaaa
     4441 attcgtcgcg ccaccggcgg gtttttgtcg aaatatgtca tcagcccctc gtagacagcg
     4501 gagttgacga tgccgtcgat ctcggtgttg acgagcttga ccttggtttg cgactcgaat
     4561 cgcgggttcg gcagcttcac gctcagcacc gcaatcaaac cttcgcgcac atcctctccg
     4621 gaaatggcag gatccttgtc tttgagcaga ctgttctgct tcgcgtactg attcacagct
     4681 ttcgtcaggg cagtgcggaa tccagtcagg tgcgtgccgc cgtcggggtt cgcgattgaa
     4741 ttcgcgaagg gcaaaatctg gtcgttgtag ctgtcgttgt attgcagcac aacatcgaca
     4801 aatacttcct cgcgctgccg cgcaatgacg attggcttgg ggtggagcac ctgcttgttt
     4861 tcgccgagct gtttgacgaa ctcctcgatg ccgtgcttgt agagaaacgt ctccttcttc
     4921 ggcgactcat cgcgttcatc ggtcaggttg atctcaagac ccggattcag aaaggccagc
     4981 tcgcgcaaac gcgtcgcgag ccgctcgaac ttaaactccg ttgtgatggt gaagatgatc
     5041 gggtcaggca aaaatgtgat cagcgtgccg gtggtctttt tgttcttaac ttcgccgatg
     5101 atttcgagct tctgcgtcgt tttacccttc gcgaaagcca tgtggtaaac cttgccgtcg
     5161 cgcgagacct ctaccttgaa ccattccgag agcgcgttcg tgcactttgc tccgacgccg
     5221 tgcaggccgc cggaatattt gtaagcgccc tggccgaatt ttccgccggc gtgcagattc
     5281 gtcagcacca gctcgactgc cggcattttc catttcggat gaatgtcgac tggaatgccg
     5341 cggccgttgt cgcgcactga aacgctgccg tccacgtgca ccgtcacttc gatttgatcg
     5401 caaaaacctg cgagatgttc gtcaattgaa ttatcgacga cctcatagac catgtggtgc
     5461 aaaccgcgct cgtccggatc gccgatgtac atgcccggac gtttccggac cgcttcgaga
     5521 ccttcgagct tgtcaatctg cgccgcgtcg tatttttgca gtttactgac gccggaaaac
     5581 tcgcggtgcc ggttttgttc gtctgaattt gcgtcgtccg aatcgacgag agcaacagct
     5641 cggcttggca ttgcaagaag gtatcgaaaa cggctcaaaa aacaaataat aatcttgtgg
     5701 ttctgaggca cttgtttgca ctaaatgtgg gatgaagcgg cactgaaaag cgcacgatct
     5761 tgtggttctc acgtctaccg cgcgagtggg attgcaagcg cttgaccgcc ctcttcgcgg
     5821 cctcgaaaat gtggctgcgc gatgcgcttc caaacacttc ttgaagcccg ctggatcttc
     5881 gcggctctcg cgttcttaac tgtcgttagc tcggtgattt ttccgccact ggcttttggt
     5941 tttttgctgt tgattctcta cacatttttc tttttccgcg acccagatcg cgccgcgccg
     6001 gaggatcccg gcgccattgt cgcggctgct gacggaacag tggccgagat cgccgaagtt
     6061 tacgaattgc agattttgaa gacgaagaca cagcgtgtcg caatcttctt gtcgatcttc
     6121 gatgtccaca ccaatcgagc accgattgaa gggcgcattt tttatcgtca acatcgtgaa
     6181 gggctctgcc tcgatgcacg cagcaccgat tgctcggcaa aaaatgaagc gatgacgtgg
     6241 gcatttgaga acccgcagac tacaattgtc gtcagacaac tgactggtgc gatcgcccgg
     6301 cgcattgtgg catggtctga tgtgggcgat tatgtcgaga agggtgagcg cttcggaatg
     6361 attcgcttcg gttcgcgcac cgaagtatat ttgccgctca ccgccaccgt gcttgtgaaa
     6421 gtgggcgatc acgtttccgc aggatcgaca atcattgcgc gattgcccga gccatgagcg
     6481 agcataattc gaaaatttac cttttgccga acctgatgac ggctgggaat ttattctgcg
     6541 ggttcactgc cacgctcaaa atcctcgaag gtgcgctgct gcaaggcagc aatccggaag
     6601 ccgcggccga cctgttccat gctgcggttt ggtttattct cggcgcgttt gtgttcgatt
     6661 ttctcgacgg acgcttggcg cggctgggcg ggaatgaaac cccgtttggt cgcgaattcg
     6721 attccctcgc tgacatcgta tcgttcgggc tggcgccggc attaatggtt tatcgaatcg
     6781 tgctgcaaga gtttccgcgc gcgtgctgga tcatcgcgtt catttatctc gcctgcggcg
     6841 ccttgcgcct cgcgcgattc aacactgccg ccatcaaccg caccgggggc gatggcacaa
     6901 ccttcaccgg tttcccgatt cccgcggccg ccggtctcat cgcgtcgatc acgctgttcc
     6961 tcctctggct ggctgagggc gaacatcggc ttggccggtg gaaattcgtg ctgccgccgc
     7021 tgctgctctt cgtttccttg atgatgttca gccgttttca gtatccgagc ttcaaggcga
     7081 tcaattggaa gacgcagaag tcgatcccgc gtttcctcgc gatcattttg attttgattc
     7141 tcaccgtcct gaatcccgaa tggatgccag cggtgctttt cctcgcctac ttgctctacg
     7201 gctttttgcg accatggctc tcacacgagt ggcgtcgcga gattgaagaa gaaatcggcg
     7261 aagaaccgtc ggaacaagcc gccgagcaaa tgccgtaaaa tcaagttttc cggctgcgcc
     7321 actcgccgct aagatcgaca tcgtgaaatc gagcgcgatg atatggctgt tggcccttag
     7381 tttggtggcc actatgcgtg cccctgcgca accgccggca ccgcgcaatc cggatgtgtc
     7441 caacaaggcg ttgatcacga agacgaggcc aagaatcgac gcgacccgca gcacgagcgc
     7501 agacatcgcc ctgatgcgca agatggcgga cgattactat gcctggcgca atgaaaatta
     7561 cccagtggca agtagcgacc gcggcctgca tacgtgggac gatcggctca cggactattc
     7621 gccagcacaa gtcgccgagc gcgcgcggca tgtccgctcg ctcctggaca aagtccgcgc
     7681 actcaagatc gacaagtggc cgaaggatga tcgtgtcgac gcgattttgt tccgggcgca
     7741 actcgagagc gtcgagttcg gagatcgggt cctgaagttc gagcggacca atccgcaggt
     7801 ttatgtcgcc gagtgcgtga acggcatttt ttcgctgctg aaaaaagatt acgacacacc
     7861 gcgcaaacga gcgctggcag cgacggcgcg cctgaaagcg atgccgccaa tgctaaggca
     7921 gggcctgagc aatttgcaaa agccggtgaa actctacgcg cagcttgcca tccagtccgc
     7981 ccgcgctatg gacccgctgc tcaatcagag cttgatggcg ttagatgtcg atcttccgcc
     8041 taacgagcac gacgagctgg tgaaatcgcg cgacgccgcg ctcgcggcag cgcacgggtt
     8101 cgcggacgaa ctcgagaaac gcctcaacaa aatggtcgac ttcgcgccga tgggcgaaac
     8161 gaactacaac tattatctca agcacgtcct gcttctgcca ttgagcgccg cggaagtgga
     8221 gatgatcggg cgcgccgagc tggcgcgtta ccgcgctctc gaagcgatgc ttccggatcc
     8281 gaaactggcc gatccagacc cgaaacgcag cggcaatatt ccgccggacc aggaagcgtt
     8341 tcttatggcg tacgagagcc gcgaaaccga gatgatcaat ttcctgaaag aacataacct
     8401 cgtcacgttg ccggattatc tcgggccatt ccaaatccgt cagctgcccg aagcatttaa
     8461 gccgaccagt ccgggggggt tcatgaatcc gcccggcgtt tacgataagg atccgacggg
     8521 gttctacttc ctcccgagct acaacccgga gtcgaagaac ttttatatac gcgccgcaat
     8581 cgaagatccg cggccaatac tcgggcatga aggaatcccg ggacatttcc ttcaactgtc
     8641 gatcgcgaac catctcactg acgaaatccg ccgccagcat gaggatggcg tgtttgtcga
     8701 aggctgggcg ctttacggtg aagaaatgtt aatgcgcacc ggcctttacc cgaataactc
     8761 accggcacag gggcaaatct tgcgactatc gcgctaccgt gcggcgcgca tcggcgtgga
     8821 cgtgaacctg cacaccggca ggtggagctt cgagcaggcg gtcacttatt tcatggaagc
     8881 cggcgggctc gatcgcgaag cggccgaggg cgaagccgcc ggcgccgctt ccaatccgca
     8941 tcagaaaatc agctatatca tcggcaaatg gcagattatg aatttgttag gccgctacaa
     9001 agatcggcaa ggagataatt tccggctcgg ccaatttcac gacgacctga tcaagaacgg
     9061 gagcctgccg gtctcggtca tcgaatgggt tttgctggac gacccggctt cggtcgaaaa
     9121 agcattgcgc tgagacaggc gacggcaatg cggaatagcc gcttgtggat gtcaatgttc
     9181 gctgccgccg tcgcgatgat tgtcgacggg tgcgtcaacc atccacgttt cgctgcgccg
     9241 ccgcgcttcc tggagcttta ctccgaagca cgcgttgcga cgctgcattt tccgcgtggg
     9301 tcgtatgcgt tcgattcgga ggacagccgt ggttactatt accgcgcgcc aagcggtgtg
     9361 ctcgagcaca ccgcggcagg accgcggcgg cgtgagggcg gtattttcgt gagcaaacgc
     9421 aatcgcgacc gactgcgcgg ctacgtcatc atgccgtacg gtctcacgca tgtgggcaat
     9481 ctctctcgca ccgagcacga gttccgggat tgattcctag tcgcgtttga ccaaatggat
     9541 gccctagaac ctccagcgtg cgagggaaag gctttgaccc gagcttgcga ccggatgcgc
     9601 ctaatccgct gacctggttc tcaatgatgc gagctgctct acgatctcca gtcgcctgtt
     9661 ctcatcggca gtacggtagt cagccaaagc cgcggccaat ccatcttcgg cgtcgtcgac
     9721 acgccggacc aacgcgagag tcgattaatt gctgccctat tcgaacgcgc tcgacagagc
     9781 cttccacagt tttccggaga cgacgttctc gtttcagatc acgtcgccga ctgaaatgtt
     9841 cggcgggatg gtgctcaaat cttggggtgt gaggaaatcg cccaccgcga tcgcggaagc
     9901 gggcgcggac gcggttacgt ccggtgctca tgacaagcgt cgccacggtc tgcg
//
LOCUS       DASTDG010000115         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_74140, whole genome shotgun sequence.
ACCESSION   DASTDG010000115 DASTDG010000000
VERSION     DASTDG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74140"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFO30_03605"
     CDS             <1..920
                     /locus_tag="VFO30_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET9418403.1"
                     /translation="PGPALANDESRPVITVTGARPGPVLFVNAGVHGGEYPAIEAVIR
                     LSKMLDAQTIAGTVILMPVLNLPAFRNRTPFVCPVDNVNPNRVFPGDPNGSYSEQMTH
                     ALINQFVVHADAYVDLHGGDIPEALVPFCICRGGNEPIDAKSKELALAFGLPYVLTVM
                     KPVQAAKGLSSYAAAAAKGVPAILAEAGGVGQLHEDAIELLTNGVKRVMRHMKMLANE
                     QALDTSASTTPTVLTSFEWVYSKHAGMWYPRVAAGDLVEKGQEIGIVGDLFGDTLETI
                     IAPVSGHVLFLTINPSVQANGLLMGIACN"
     gene            1162..2523
                     /gene="gdhA"
                     /locus_tag="VFO30_03610"
     CDS             1162..2523
                     /gene="gdhA"
                     /locus_tag="VFO30_03610"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET9418404.1"
                     /translation="MENYVSNVMAGVKAKNPGELEFQEAVAEVVKSVTLVLDRHPQYR
                     KAKILERMIEPERAIMFRVAWQDDAGELQVNRGFRVQMNSAIGPYKGGLRFHPTVNLS
                     ILKFLAFEQVFKNALTTLPMGGGKGGSDFDPHRKSDAEVMRFCQAFMCELFRHIGPET
                     DVPAGDIGVGAREIGFLFGMYKKLKNEFTGVMTGKGLTWGGSVIRPEATGYGAVYFAE
                     EMLKTRKEDLKGKTALVSGSGNVAQYTVEKLISLGAKPITLSDSTGYIFDEAGITRDK
                     LAFVMDLKNVRRGRISEYADKFKGAVFTPVNSKLDHNPLWDHKAQCAFPSATQNEING
                     KDAANLLKNGVYVVCEGANMPSTIDAINQFLDAKILFGPGKAANAGGVATSGLEMAQN
                     SMRIRWTREEVDARLFNIMKTIHEVCQRTAEKYGTPGNYVNGANIAGFVKVADAMLDQ
                     GLV"
     gene            3109..3687
                     /locus_tag="VFO30_03615"
     CDS             3109..3687
                     /locus_tag="VFO30_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9418405.1"
                     /translation="MGEETATAQQIADREMIARIGRGDQSAFSALYDRLSGPLYSLAL
                     RMLRDSADAQEALQDVFLQIWARAGTYDPEQSSVFSWTVLLTRSRVIDRLRARDRRLR
                     LAVVSTDDKESRADASTIESAADTADRNDEAARVRFVLNNLPSEQREAIELAFFEHLT
                     HQEIAAQLGQPLGTIKARIRRGLLKLRQRLRI"
     gene            3690..4406
                     /locus_tag="VFO30_03620"
     CDS             3690..4406
                     /locus_tag="VFO30_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9418406.1"
                     /translation="MISEEQQDQAAVYVLGLLDSAEAGAFESALAADPALRDFVRELR
                     DAAGDLALSAPAHTPPASLKQRVMREIAIKAKPSASVSIQRTALTWLPWAIAAGLIVF
                     CGVLALDRFRLQRELTDVRATDPLMQTIFFTLSPSPPAPADAKVTVAWQPSRQSGVIR
                     ITNLPAPQPGRDYQLWAVDAAHKDPISAGVIRVDENGVAQLRFRPVDRAQQVKAFAIS
                     LEREGGVPKKEGPILFAGTA"
     gene            4528..>5617
                     /locus_tag="VFO30_03625"
     CDS             4528..>5617
                     /locus_tag="VFO30_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418407.1"
                     /translation="MKTGKQAANERAEKHADKLLVSIPLLLLVACLLFVSYWSKREIQ
                     ASKTGAPVTVSLDPLALVLTPQTGNSRTDKEICRLQQQIRDGRNPELALEQLGWAFVA
                     KARESFDAGFYKLAEQCARCIEKRNPHSHEAMLLRGHVLHNLHRFKESETLARQLVEQ
                     RGLNFDYGLLGDALMEQGKLNEAVAAYQQMMNLKPDLHAYARAAHMRWLKGDLAGAIE
                     VMQLAVDAASPLDAESAAWVNTHLASYQFQAGRFAEAEQRCAVALSFQSNYPPALLLR
                     GKMLLAQDKFEAATDDLQTAAKLNRLPEYQWALADALRAAGQINEASSSEAELRRLGA
                     TSDPRTLAIFLSTRHESPEVALRLAKAEF"
BASE COUNT         1219 a         1573 c         1693 g         1132 t
ORIGIN      
        1 tgcccggtcc ggcgctggca aatgatgagt cgcggccggt gataacagtc acgggcgcaa
       61 ggccgggtcc ggtcttgttc gtgaacgcgg gggtgcatgg cggtgagtat ccggcgatcg
      121 aggcggtgat ccggttatca aaaatgctcg atgcgcagac gatcgcgggc acggtcattc
      181 tcatgccggt gttgaatctg ccggcgtttc ggaaccggac gccgttcgtt tgcccggttg
      241 ataacgtgaa cccgaaccgc gttttcccgg gcgacccgaa tggcagctac agcgagcaga
      301 tgacacacgc gctgatcaac cagttcgtcg tccacgccga tgcatatgtc gatcttcacg
      361 gcggcgatat tcccgaagcg ctcgtgccgt tctgcatttg tcgcggcggc aacgaaccga
      421 tcgacgcgaa atcgaaggag ctcgcgctcg cgttcgggtt gccgtacgtg ctcacggtca
      481 tgaaaccggt gcaggcggcg aaaggtttat cgagctacgc cgcggccgct gccaaaggcg
      541 ttccggcaat tctcgccgaa gccggtggtg tcgggcagtt gcacgaagat gcgatcgagc
      601 tgctgacgaa tggagtgaag cgcgtgatgc ggcatatgaa gatgctcgca aacgagcagg
      661 cgcttgacac aagcgcctct acaacgccga cagttctcac ctcattcgaa tgggtctatt
      721 cgaaacatgc aggcatgtgg tatccgcgcg tggccgcggg cgatctcgtc gagaaaggac
      781 aagagattgg catcgttggc gatcttttcg gcgacacact ggaaacaatc atcgcgccag
      841 tcagcggcca cgtgctgttt ctcaccatca atccgtccgt gcaggcgaac ggattgctca
      901 tgggcatcgc gtgcaactga atctggaaac catgaacccg acgatcctct ggagcgatct
      961 gcggttcctt cctactttcc tggttcctga ttaacctgtg caaatctgtg ttgatctgtg
     1021 gctgatggaa acactgaatg atccgcagat ttcgcggatt aacgcagatt tctttttttg
     1081 ttgcttcctg attagccgct ccggtcttgt atccgctaca acatacgtct aagttgcgcg
     1141 tctcttcgcg ctgaggctcc aatggaaaat tacgtgtcga atgtgatggc cggggtaaag
     1201 gccaagaacc cgggggaact ggagtttcag gaagcggtcg cggaagtcgt caagtcggtc
     1261 accctggtgt tagatcgaca tccgcagtat cggaaagcga aaatcctcga gcggatgatc
     1321 gagcccgagc gggcgatcat gttccgcgtc gcctggcagg atgatgccgg cgaactgcag
     1381 gtgaaccgcg ggttccgcgt gcagatgaac agcgcgatcg gcccgtacaa aggcgggctg
     1441 cggtttcatc cgacggtgaa cctcagcatt ctgaagttcc tcgcgttcga gcaggtcttt
     1501 aaaaacgccc taacgacttt gccgatgggc ggcggcaaag gcggttctga tttcgatccg
     1561 cacagaaaaa gcgacgcgga agtgatgcgg ttttgtcagg cgttcatgtg tgagctgttt
     1621 cggcacatcg gaccagagac ggacgtgccg gcgggcgaca tcggcgttgg cgcgcgcgag
     1681 atcggttttc ttttcggcat gtacaagaag ctcaagaacg agttcaccgg tgttatgacg
     1741 ggaaaaggtc tgacctgggg cggctcggtg attcggccgg aggcgaccgg ctacggcgcg
     1801 gtttatttcg cggaggaaat gttgaagacg cgcaaggaag acctgaaagg caagaccgcg
     1861 ctggtctcag gcagcggcaa cgtcgctcaa tacacggtcg agaagctcat ttcgttaggc
     1921 gcgaagccga tcacattgtc ggattcgacc ggctatatct ttgatgaagc cggcatcact
     1981 cgggacaaac tcgccttcgt gatggacctg aagaacgtcc gacgcggccg catctccgaa
     2041 tacgccgata aattcaaggg cgcggtgttc acgccggtca actcgaagct cgaccacaac
     2101 ccgctctggg atcacaaggc gcagtgcgcg ttcccgagcg cgacgcagaa cgagatcaac
     2161 ggcaaagacg cggcgaatct tttgaagaat ggcgtttacg tggtctgcga aggcgcgaac
     2221 atgccaagca cgattgatgc gatcaatcag ttcctcgacg cgaagattct cttcggtccc
     2281 ggcaaagccg cgaacgcagg cggcgttgcc acctcgggac tggagatggc gcagaacagc
     2341 atgcgcatcc gttggacgcg cgaggaagtc gatgcccggc tgttcaacat catgaaaacg
     2401 attcacgaag tctgtcagcg cacggccgag aagtacggta cgcccggcaa ctacgtgaac
     2461 ggcgccaaca ttgccggctt cgtaaaagtg gccgacgcaa tgctggacca ggggctggtc
     2521 tgataattcc gacaatttat cttgccatcc tgagcggagc ccaatcgact cgcctcgctc
     2581 gctcagggta aactctgcgg agtcgaagga cctgacgaaa tgactcgaga ccatcagtat
     2641 ttagtttaca tgatgagcaa caaaacacgc tcggtgctgt acacgggcct gactaatgac
     2701 ctgcgggcgc gagtagcaca acatcgcacg gcggaatacg gggttgcacc cgcgattatc
     2761 gttgcatagc gctcgtgtat tgctcccgtt ttggcgacat tcaccaagca atcgcgcggg
     2821 agaagcagtt tgcgctggcg acggtcgaag aagaacgcgc tcatcgagtg tgacaacgcg
     2881 aactggaagt ttattttcaa actggcagtc ggctgcggcg aacgccccga tgtgcgcgtg
     2941 gactgactgt ctgcgcggcc tttacagatc gccgcaaatg cagttcgcgg cgtgaatcta
     3001 gaacatgcag cgacaaaatc gttgcatcca gacgcgacga tttgtcggat gcctcgaaag
     3061 gtgttttagc caccgtgaga aacaaactaa cctcgacttg atctgagcat gggggaggaa
     3121 acagccacag cgcagcagat tgcagatcgt gaaatgatcg cgcggatcgg ccgtggagat
     3181 caaagtgcgt tcagtgcgct gtatgatcgg cttagcggtc cgctttattc tttggctttg
     3241 cgcatgctgc gggactccgc cgatgcgcag gaggcgctac aggatgtttt tctgcaaata
     3301 tgggcccgcg ctggaactta cgatcctgaa caaagcagcg tctttagctg gacggttctg
     3361 ttgacgcgaa gcagggtgat cgatcgattg cgagcgcgcg accggcggtt gcggctggcg
     3421 gtggtttcga ctgacgacaa agagagtcgc gccgatgcat ccaccatcga gagcgcagcg
     3481 gataccgcag acaggaatga tgaggcggcg cgtgtgagat ttgtattgaa caatttgccc
     3541 tccgagcaac gagaggcgat tgaactggcc tttttcgagc atctcaccca ccaggaaatc
     3601 gccgcgcaac tcgggcaacc cctcggtacg atcaaagccc gaatccgccg cggccttctc
     3661 aaactacggc aacgacttcg catctgatta tgatttcgga ggaacaacag gatcaggcag
     3721 cggtctacgt gctcggcctg ctcgactcgg ctgaagctgg tgcgttcgaa tcggcgctcg
     3781 ccgcggaccc tgcattgcgc gatttcgttc gcgaactgcg cgacgcagcg ggcgacctcg
     3841 cactgagcgc gccagcgcat acgccgcctg cctcattgaa gcaacgcgtg atgcgcgaga
     3901 ttgcgatcaa agcaaaaccg tccgcttcgg tgtcgatcca acgtactgcg ttgacctggc
     3961 taccgtgggc aatcgccgcg ggtttgatcg tcttttgcgg cgtgctcgcg ctcgatcgct
     4021 tccgcctgca gcgcgaactg actgatgtgc gcgccacgga tccgctaatg cagacaatct
     4081 ttttcactct cagtccttct ccgcctgcgc cagcggacgc aaaggtgacg gtcgcgtggc
     4141 aaccgagccg gcaaagcggc gtgatccgaa ttacgaacct gcccgcgccg cagccaggca
     4201 gggattatca actctgggcg gttgatgccg cgcacaagga tccgattagt gcaggcgtta
     4261 ttcgcgtgga cgagaacggt gtggcgcaac ttcgcttcag gccggttgac cgggcgcagc
     4321 aggtaaaagc ttttgcgatc agcttggagc gcgagggtgg cgtccccaag aaagaaggac
     4381 ccatcttgtt cgccggcact gcctgaatat tcctcgagcg aggatcagcc ggattcgctc
     4441 gctcgaaaac ttgcagcgct cagctgtctt tagcgttttg gatgcatccg ctcgtgatgt
     4501 ttcagcgtaa cgcttgtcca aacaataatg aaaacgggca aacaagccgc caacgagcgc
     4561 gcggagaagc acgccgataa actcttggtt tcaattccgc tgcttctgct cgtcgcctgt
     4621 ttgctgtttg tttcctactg gtcgaagcgc gaaattcagg cgagcaagac cggcgcgcca
     4681 gtcacagttt cgcttgatcc gctggcattg gtcttaacac cgcagacagg taacagccgc
     4741 accgacaagg aaatttgccg gctccaacag caaatccgcg acggcaggaa ccctgaactc
     4801 gcgctcgaac agctcggctg ggcgttcgta gcaaaagcgc gcgaaagctt cgacgcgggt
     4861 ttttacaagc tcgccgagca atgcgcgcgg tgcattgaga aacgcaatcc gcacagccac
     4921 gaggcgatgc tgttgcgcgg acacgtcctg cacaacctgc atcgcttcaa agagtctgaa
     4981 acgctggcgc gacagttggt cgagcaacga gggcttaact ttgattacgg tcttctcggc
     5041 gatgctttga tggagcaagg caagctgaac gaggcggtcg cggcttatca gcagatgatg
     5101 aacttgaagc ccgaccttca cgcctacgcg cgcgccgcgc atatgcgctg gctcaaaggc
     5161 gatctcgccg gcgcgatcga ggtgatgcag ttggccgtgg acgcggccag cccgctcgat
     5221 gccgagtctg ctgcgtgggt gaatacgcat ctggcttctt accagtttca agccggccga
     5281 tttgcagaag cagagcaacg gtgcgctgtc gccttatcgt tccagagcaa ttaccccccc
     5341 gcgctgttgc tgcgcggcaa gatgttgctt gcccaagaca aattcgaagc ggccaccgac
     5401 gatctacaaa cagcggcgaa gttaaatcgg ctccccgaat atcaatgggc gctcgccgac
     5461 gccctgcgtg cggcgggcca gataaacgaa gcgtcctcga gcgaagctga actccgacga
     5521 ctaggcgcca cgagcgatcc gcgcacatta gcgatattcc tctcgactcg gcacgaatct
     5581 cccgaggtcg cacttcgact ggccaaggcc gaattcg
//
LOCUS       DASTDG010000116        10446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1242010, whole genome shotgun sequence.
ACCESSION   DASTDG010000116 DASTDG010000000
VERSION     DASTDG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242010"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFO30_03630"
     CDS             complement(<1..1356)
                     /locus_tag="VFO30_03630"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9418408.1"
                     /translation="MQQKVTAQIGATQVSIETGKIAKLADGAVIVSCGDTMVLASAVS
                     AATIKEGQDYFPLTVDYREKAAAAGKFPGGYFKREGRPTEKETLTSRMIDRPLRPLFP
                     PGYFYDTQVISLLLSADGENDPDILAINGASAALCISDIPFAGAIGAVRVGRVNGQFV
                     ANPTHSERAQSDLDLVYVGTHEEIIMIEGAANQVAEADFIDALEFAHAHAGEMIRIQK
                     DLAAQFGKPKREMPLLQINHELLDIAYQIAGDRIEDALYTQGKVARGKAVEALREEVK
                     MSILEKHPQADTFAIAQAFDHVQKKAVRRSILEKQKRMDGRGYADLRAISCEVSVLPR
                     AHGSAIFQRGETQAVALATLAPIEEAQMLDAYGGGEQSKRFILHYNFPPFSVGETGRT
                     GGASRREIGHGALAERSLEPVVPSEHDFRYAIRISSEIMESNGSTSMASVCGGMLALM
                     DA"
     gene            complement(1531..1794)
                     /gene="rpsO"
                     /locus_tag="VFO30_03635"
     CDS             complement(1531..1794)
                     /gene="rpsO"
                     /locus_tag="VFO30_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9418409.1"
                     /translation="MAEKMETLDLKEFQLHEKDTGSADVQVALLTRRIEHLTEHLKTN
                     AKDRSSRRGLLRMVAQRRSLLDYLSKTKADRYKNLIDKLNLRK"
     gene            complement(1919..2176)
                     /locus_tag="VFO30_03640"
     CDS             complement(1919..2176)
                     /locus_tag="VFO30_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9418410.1"
                     /translation="MLHIIWSIIVGFIVGLIARAIMPGVQHLGFIATTLLGIGGSIIG
                     GLIARIFSPPAPGSGFHPAGFILSIIGAVILLFIWARLNPA"
     gene            complement(2333..2674)
                     /locus_tag="VFO30_03645"
     CDS             complement(2333..2674)
                     /locus_tag="VFO30_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9418411.1"
                     /translation="MKNENPWIEICPGIRRQTVAHGKTMYQMIAELEAGSVMPAHQHP
                     QEQIVHLLSGRMKLVVDGTPHELRAGDSFYLASNVPHGVETIEQTRVLDTFSPPRDEY
                     LALDEEARKEL"
     gene            complement(3377..4114)
                     /locus_tag="VFO30_03650"
     CDS             complement(3377..4114)
                     /locus_tag="VFO30_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418412.1"
                     /translation="MLSFIVPAYNEEIELPATIESIRAAAHNIDPLRGGFAVANNYEI
                     IVVDDASTDATAEIARKAGAEVVAIDRRQIAAARNAGARAARGDILFFVDADTRIRRE
                     HVTGAIEAVNGAYAGGSARVVTDGTIPLWGRLLMKTFCAVYFANNLGAGAFLFTTREN
                     FDKIGGFNEDFYIGEEVYFSLALRKLGRFKILRTPVITSGRKLRMYSARQILVRSFGI
                     LLHGKRAALSRDRLDLWYEGKRETKST"
     gene            complement(4173..6719)
                     /locus_tag="VFO30_03655"
     CDS             complement(4173..6719)
                     /locus_tag="VFO30_03655"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9418413.1"
                     /translation="MGRIRPLGSILLAGLLLLSASSLPAERKPVLSQIHLPHSYYYRE
                     MYLPQLTTGPSALTFSPDGKELVYSMAGSLWRQTIGSDQAHELTHSAGYDYQPDWSHD
                     GRHIIFTRYLNDALELWQLDLHTGKEVALTSHGDVNVEPRYSPDDRHIAFVSTAGTGH
                     FNLYVAELDGDRLIGSRRLLPEHISTVYRYYYAPTDHSINPCWTPDGQRLLLVSNREV
                     AYGTGDIWSVAVNAPEDAKKILSQETTWRAQPQVAPDGRRVLFSSYHGRQWQQLWLTT
                     VEGAAPLPLTFGEFDRTQARFSPDGSRVGYISNEEGNTSLWVQEIVGGARTRVNANHR
                     QYLHEVGRLQLTVRDEHGNSVPARVSVIAADGRAYAPDNAWMHADDGFDRDVQPFENH
                     YFHCPGECTLTLSTGDARLQVFHGMDYRIAEQNVTIRTGENNTTIRLEPLRLPTQYGH
                     FASADLHVHMNYGGHYRNDPDHLIEQARAEHLDAVYNLIVNKEERVPDIAQFTPRPLR
                     KEGVLLMQGQEFHSSYWGHLDLLDLDDHFLTPDFSAYRESAFSSPYPHNGVVARLTHA
                     QHGLVGCAHPFDEPVNPEKDLHLTNTFPVEIALGGADYYEVVGFSDHRATADIWYRLL
                     NLGFRVPAGAGTDAMANYASLRGPVGVNRVFIAISGEPTPEKLHSGLKQGRTFATNGP
                     LLGLEINSRHPGDEITLDKPTTVPYHAALRSIVPIDHFELVFNGKVVATPRLEGARTQ
                     TDVSANVEIPGSGWLVLRAWNDQADPKIQDIYPYASTSPIYVTVNRQPPRSPEDATYF
                     VAWVDRVIANASARSDYNSEQEKRDTLDYLNTARTAFSTRTETARSPVSR"
     gene            complement(6719..8023)
                     /locus_tag="VFO30_03660"
     CDS             complement(6719..8023)
                     /locus_tag="VFO30_03660"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET9418414.1"
                     /translation="MRTELPGPKARALLARDAEVVSPSYCREYPFVMSHGRGVEVWDV
                     DGNRFLDFAAGIAVCSTGHSHPQVVRAIKDCADQFLHISSDFVHERQVALGEKINALS
                     PMGEPVLSFFCQSGTEAVEGALKLARYVSGRSRFIGFLGGFHGRTMGSLAFTASKCTQ
                     QQGFATMPGVTHVPFPNPYRPLFAGDDQGQAVLNYIENVLFVSNVPASEVAAILVEPI
                     QCESGYVVPPDGFLGGLRQLCDRHGILLILDEVQSGVGRSGKMFACQHWNVQPDIMTL
                     AKGLGSGLPIGLVVAKKNIMQQWKPGAHANTYGGNPLCCAAALVTLDLIEREYVHNAE
                     KVGVHFMTRLHELQSRHPVIGEVRGKGLMLGMELVSDRASRAPCKGSCDAVIKRAFHR
                     GLLLLSCGQSTVRFMPPLTVSVAQVDEAITLLDAALLEALSQ"
     gene            complement(8050..8841)
                     /locus_tag="VFO30_03665"
     CDS             complement(8050..8841)
                     /locus_tag="VFO30_03665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9418415.1"
                     /translation="MMTIAAARLIPPGCVCFVGIGIPSAAANLARLTHAPDIVLVYES
                     GSIGTEPKVLPLSIGDGELAETAAAVVSLPEIFSYWLQAGRMDLGFLSAAQIDRFGNL
                     NTTVIGDYRRPKTRLPGAGGAPEIAQHAKQIFIVLKQSPRSFVANLDFCTSAGFLTGR
                     GERARREISGAGPQVVITDLGILKPASGSEELTLVASYDGISVGRICAATGWPLAVAD
                     TVEVVPPPTLGELCVLRDLQKRTQVAHAGSVLAQREMHRLLQTQV"
     gene            complement(8883..9728)
                     /locus_tag="VFO30_03670"
     CDS             complement(8883..9728)
                     /locus_tag="VFO30_03670"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9418416.1"
                     /translation="MQLGTLQEVIDEYVDDGQSVALEGFTHLIPFAAGHEILRQKKRE
                     LHLIRMTPDLIYDQLIGAGCARELTFSWGGNPGVGSLHRLRDAVENDWPQPLKINEHS
                     HAGMAAAYTAGAARLPFGMLRGYVGTDLSQVNGQIREVHCPYTDAAVAAVRAINPDVT
                     VLHAQAADRQGNVLIRGIVGAQKEAALAARTLIVTVEEVVESLSAPMNAIVLPHWVVT
                     AIAVVPMGAYPSYAHGYYARDNAFYRRWDEIARDRDTFTNWITRHVVRSRDHAEFLDS
                     LAEAA"
     gene            complement(9732..>10446)
                     /locus_tag="VFO30_03675"
     CDS             complement(9732..>10446)
                     /locus_tag="VFO30_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9418417.1"
                     /translation="EMGGKNACIVTVNADLDRAAAGIVRSAYGLSGQKCSALSRIYVD
                     EKIADPLIDKLTEKIAGITIGDPTRQENWLGPVTTANALHKYERYSKQLAENGARILC
                     GGRRLTERELAHGYFVAPTLAEAPANHPLFAEEMFVPIAMLCRVRDREEAIRLANDTP
                     YGLTAGCYGNDDDATYFFDNIEAGVTYVNRPQGATTGAWPGYQPFGGWKGSGSSGKGI
                     ASFYYLAQYQREQSQTKVE"
BASE COUNT         2175 a         3069 c         3008 g         2194 t
ORIGIN      
        1 cgcgtccatc aacgcaagca ttccgccgca gacgctcgcc attgaggtcg agccgttcga
       61 ttccatgatc tcactcgaga tacggatggc gtagcgaaaa tcatgttcgc tcggcacgac
      121 cggctcgagc gaacgttctg ccaatgcgcc gtggccaatc tcacgccggc tcgctccgcc
      181 ggtgcgaccg gtttcgccca cgctgaaggg aggaaaattg taatgcagaa tgaaacgctt
      241 cgattgttcg ccgcctccgt aggcgtcgag catctgcgct tcttcaattg gggccagtgt
      301 ggcaagcgcg accgcctggg tttcgccgcg ctggaaaatg gccgagccgt gcgcccgcgg
      361 cagcacgctc acttcacaac tgatcgcgcg caggtcggcg tagccgcggc cgtccatgcg
      421 tttctgtttt tccagaatgc tgcgacgcac cgctttcttc tgcacgtggt cgaaggcctg
      481 cgcgatcgcg aaagtgtcgg cctgcggatg cttctccaag atcgacatct tcacttcttc
      541 gcgcagcgct tcgacggctt tgccccgcgc gactttgcct tgcgtgtaaa gcgcgtcctc
      601 gatccgatcg ccggcgatct gataggcgat gtcgagcaac tcatgattga tctgtagcag
      661 cggcatttcg cgttttggtt tgccgaactg cgctgccaaa tctttctgga tccgaatcat
      721 ctcgcccgca tgagcatgcg cgaactcgag cgcgtcgatg aaatctgctt ccgcgacttg
      781 gttcgccgcg ccttcgatca ttatgatctc ctcgtgcgtt ccgacgtaaa ccaaatcgag
      841 atcgctctgc gcgcgctccg aatgcgtcgg attcgcgacg aattggccgt tcacgcggcc
      901 gacgcgcaca gcgccgatcg cgccggcaaa cggaatgtcg gaaatgcaaa gcgctgccga
      961 cgcgccgtta atcgcgagaa tgtcaggatc attttcgcca tcggcgctca agaggaggct
     1021 gatgacttgc gtgtcgtaaa agtaacccgg cggaaaaagc ggccgcagcg gccgatcgat
     1081 catgcgcgaa gtgagtgttt ccttttccgt gggtcgacct tcgcgtttga agtaaccgcc
     1141 cggaaatttt ccggcggcgg ccgctttctc gcggtaatcg acagtgagcg ggaagtaatc
     1201 ctgaccttcc ttgattgttg cggctgagac cgcgctggcc agcaccatcg tgtcgccgca
     1261 cgacacgata accgcgccat cggcgagctt ggcgattttg ccggtctcaa tggagacctg
     1321 agtggccccg atttgggccg tgaccttttg ttgcatatat cctcctggat gtgagctcca
     1381 ggccgggtca ccttgactgt aggttttaat cccgcaggcg ttcgagccta aaacctaaat
     1441 ccaagatggc tacggcggcc tggactctgt tgttgtttgt ttttcgcttc ggcgcgaaac
     1501 agaaacgtcg cgcgactcaa tcgagccgcc ctatttccgc aggttcaact tgtcgatcaa
     1561 gtttttgtaa cgatcggctt ttgtcttgct caagtaatcg agcaagctgc ggcgttgcgc
     1621 gaccatccgg agaaggccgc ggcgcgagga acggtccttc gcgtttgtct tcaggtgctc
     1681 ggtcaggtgc tcgatgcggc gcgtcaaaag tgcaacctgc acatcggcgc tgccggtatc
     1741 tttctcgtgc aactggaact ccttcagatc caacgtttcc attttctctg ccataaacga
     1801 agcccgtatt gtagatgaag acggcgcttt ggcaagcggt ggctctttag ccgcgcacct
     1861 atggcgcgtc ggggtcccgc gactcatgga cggccctcgg ggccgtggct acactgcatt
     1921 acgcgggatt cagtctggcc caaataaaca gaagaatgac tgcgccaatg atcgaaagaa
     1981 taaagccggc cgggtgaaag ccggatcccg gcgcaggtgg cgaaaagatc cgggcgatca
     2041 gtccgccgat gatggagcca ccgatgccaa gcagcgtggt tgcgataaag cccagatgtt
     2101 gtacgccggg catgatggcg cgggcgatta aaccgacaat gaaaccgaca atgattgacc
     2161 agatgatgtg taacatagtg tgtttccgta gttaggcgcg caggctatca ctgacacgcg
     2221 cgcaaaagcc aatactttcc gccgcgcgca agatcgacat catgtagcca cggcgctacg
     2281 cgccgttcga gttttcgttc gctccatcat ccgaccggcc tcacgccggt ggctacagct
     2341 ccttccgtgc ttcttcgtcg agcgcgaggt attcgtcccg cggtggactg aacgtatcca
     2401 agacccgcgt ttgctcgatc gtctccacgc catgcggaac attgctcgcc agataaaatg
     2461 aatcgcccgc tctcaattcg tgcggtgttc cgtcaaccac caacttcatc cggccactca
     2521 gcagatgcac gatctgctcc tgcggatgtt ggtgcgcggg catgacgctg ccagcttcca
     2581 gctcggctat catctgatac atcgttttac catgcgcaac cgtctgacgc ctgatgccag
     2641 gacaaatctc gatccaagga ttttcatttt tcatacgatt ggaatctact gtagcggcag
     2701 ctgtcctcag tcgcacattc tgcaattcct gaggagagtg aggacacgcc cctatatgtg
     2761 ccggactcgc gtcgagcgcg aaattctgta gtcgcggcgc tccgtcgtca tgcaacgcca
     2821 gtccggctcg gaccgcctcg ctacgctgga ggcaatggtc cgaccagatt atgagtgccg
     2881 atagaagact atcccgcaca gcacgcgcag gccgaacacg accgcgcaga caagcgtgcg
     2941 gagctgatcc ggccacgtga ggctccggtg ccgaagaaca accaggaccg agccgacaaa
     3001 caatagatat gtagtgatct gcgccggcca aacccaattg ccagtctttt ctcttggcag
     3061 cagcctacgc gtatctgctg tccgagacgc ggcgtcagtt ggatgctgga agaacctttc
     3121 aaaaagcggt gacatcggga gtcctgcgat ggctgcggcg atcacggcag cgacgatgat
     3181 cgcaaacacc ctaagggcag acatgcgcat ttcacggtca atcacgtgac atgtaaccgg
     3241 cgaagctcgc gtctcagaag ttccttgtcg cattaggagc gcggatttct atagccgtat
     3301 tttgtatcca aataacgagc gtcctctcac aaacgcatca gcgtaaagtc ggtgtcgagc
     3361 gcgcttttgt cgcctttcat gtcgactttg tttcgcgttt gccttcgtac caaaggtcaa
     3421 gccggtcacg tgacaacgcc gcgcgtttac cgtgaaggag gatgccgaat gagcggacga
     3481 gaatctggcg cgcggaatac atgcgcagtt tgcgcccgga agtgatgacc ggcgtgcgca
     3541 ggatcttgaa ccggccgagc tttcgcagcg ccagactgaa ataaacttct tcgccgatgt
     3601 agaagtcttc gttgaagccg ccgatcttgt cgaagttttc gcgcgtcgtg aaaaggaacg
     3661 cacccgcgcc caaattgttc gcgaagtaaa cggcgcaaaa tgttttcatc aatagccggc
     3721 cccagagcgg aattgtgccg tcggtcacaa cccgcgcgct gccgccggca taagcgccat
     3781 tcaccgcctc aatcgcgcca gtcacgtgct cgcgcctgat tcgtgtatcg gcatcgacaa
     3841 agaacagaat gtcaccgcgc gcggcgcgcg cacccgcgtt acgggcagca gcaatctggc
     3901 ggcgatcgat cgcgaccact tccgcgccag cctttcgcgc aatctctgcc gtcgcgtcgg
     3961 tcgacgcgtc gtccacgacg atgatttcgt agttgttcgc cacggcgaat ccgcccctta
     4021 gcggatcgat gttgtgcgcg gctgcgcgga tcgattcaat cgtagccggc agctcgatct
     4081 cttcgttgta cgccggaact atgaaggaca gcacaaagta gctgtagaag cgcgtggcaa
     4141 cgtgcgcaag atgtcagctt caccagcgcc gatcagcgag agacaggtga acgcgccgtt
     4201 tctgttcgcg ttgaaaacgc agtgcgcgct gtattcaggt aatcaagtgt gtcccgcttt
     4261 tcctgctcgc tgttatagtc gctgcgtgcg ctagcgttag cgatgactcg atcaacccag
     4321 gcgacaaaat aggtcgcgtc ctctggcgag cggggtggct gtcgattaac cgtgacgtag
     4381 atggggctgg tgctggcgta agggtagatg tcttgaatct tcgggtcggc ttgatcgttc
     4441 caggcgcgca acaccagcca accgctgccg ggaatttcca cattggcact cacatcggtc
     4501 tgcgtgcgcg cgccctcgag acgaggagtg gcgaccacct ttccgttgaa gaccaactcg
     4561 aagtgatcga tcggcacaat ggaacggaga gctgcgtggt agggcactgt tgtcggctta
     4621 tcaagcgtga tctcatcacc cggatgcctg ctatttatct ccaggcctag caacgggcca
     4681 ttcgtggcga aggtccgccc ctgcttcagg cccgagtgca gtttctccgg cgtgggctcg
     4741 ccggaaatgg cgatgaatac ccgattcacg cccaccgggc cgcgcagtga agcgtagttc
     4801 gccatcgcgt cggtacctgc gccggctggc acgcgaaaac caagattgag caaccgatac
     4861 cagatgtccg cggtagcgcg gtggtcggag aaacccacca cttcgtagta gtcagcgccg
     4921 ccgagcgcga tctcaaccgg aaaagtattg gtgaggtgga ggtccttctc cgggttgacc
     4981 ggctcatcaa acggatgcgc gcagccgacc aagccatgtt gcgcgtgtgt cagccgggcg
     5041 acgacgccat tgtgtggata ggggctgctg aaggcacttt cacgatacgc cgaaaaatcg
     5101 ggcgtcagga aatgatcatc cagatccagc aggtcgagat gaccccagta gctactgtga
     5161 aactcctgcc cttgcatcag cagtacgccc tctttacgca gcgggcgggg agtgaactgc
     5221 gcgatatcgg ggacgcgctc ttccttattg acgatcaggt tgtagacggc atcgaggtgt
     5281 tcggcacgtg cttgctcgat caaatggtcc ggatcgttgc gatagtgccc accgtagttc
     5341 atgtgcacgt gcagatcggc actggcgaag tggccgtatt gagttggcaa acgcaatggc
     5401 tcgagcctga tcgtcgtgtt attttctcct gtcctgatag tgacgttctg ctctgcaatc
     5461 cggtaatcca tcccatgaaa cacctgcagc cgcgcgtcac cggtcgatag cgtgagcgtg
     5521 cattcgccag gacaatggaa atagtggttc tcgaatggct gaacatcgcg atcaaagcca
     5581 tcgtcggcat gcatccaggc attgtccggc gcataggcac gtccgtcggc agcaatcacg
     5641 ctgactcgcg ccggcacgct gttcccatgc tcgtcgcgca cagtcagctg caatcgcccg
     5701 acctcatgta ggtactgtcg gtggttggcg ttgacgcggg ttcgagcgcc gccaactatc
     5761 tcctgaaccc agagcgaggt attgccctct tcattgctaa tatagccgac gcggctgcca
     5821 tcgggcgaaa atcgagcctg ggtacgatca aactcgccga acgtcagcgg caaaggcgcc
     5881 gcaccttcca cagtcgttaa ccagagctgc tgccattgac gaccgtgata actgctgaac
     5941 aaaactcgcc ggccatcagg tgcgacctgt ggctgtgcgc gccaagtggt ctcttgactg
     6001 aggatctttt tcgcatcctc gggcgcgttg actgccactg accagatatc gcccgtgccg
     6061 taggcgactt ccctgttcga caccaacagc agacgctgcc catcgggcgt ccagcaagga
     6121 ttaatagagt gatcagtggg cgcgtagtag tagcgataga ccgtgctgat gtgctcaggt
     6181 aacaaacgcc ttgagccgat caaacgatcg ccgtccaact ctgcgacata gaggttgaag
     6241 tgaccggtcc cagcagtgga aacgaaggcg atgtgacggt catccggtga atagcggggc
     6301 tcaacattga catcgccatg cgatgttagg gcaacctcct tgccagtgtg cagatcaagc
     6361 tgccacagct cgagcgcgtc gttcaggtaa cgggtgaaga taatgtggcg accgtcatgc
     6421 gaccagtctg gctgatagtc gtaaccggct gaatgggtga gttcatgcgc ctgatcgctc
     6481 ccgatggtct gacgccacag cgacccagcc atgctataaa ccaactcctt gccatcaggc
     6541 gaaaatgtca gtgctgacgg accggttgtt agctgcggca gatacatttc acggtagtaa
     6601 taactgtgcg gtaaatggat ttgcgacagt accggcttac gttcggccgg gagtgatgaa
     6661 gcagaaagta acaagaggcc ggccagcagg atgctgccta gaggcctgat cctccccatt
     6721 attgagacag cgcttcgagg agggcggcgt cgagcaaagt gatcgcctca tcgacctgtg
     6781 ctactgacac ggtcagtggc ggcatgaagc gcactgtgct ctgtccgcag gagagcaaga
     6841 gcaggccccg atgaaatgcg cgtttgatca cggcatcaca cgaccctttg cacggtgcgc
     6901 ggctggctcg atcgctgacc agctccatgc cgagcatcag tcctttgcca cggacctcgc
     6961 cgatgaccgg gtgccgcgac tgcaattcgt gcaggcgtgt catgaaatgg acgccgactt
     7021 tctcggcatt gtgcacgtac tcgcgttcga tcaggtcgag tgtcaccagc gccgccgcgc
     7081 aacatagcgg attgccgccg taagtgttgg catgggcgcc aggcttccac tgttgcatga
     7141 tgtttttttt agccacgacc aggccgatcg gcaggccgga gcccaggcct ttggccaacg
     7201 tcatgatgtc cggctgcaca ttccagtgct gacaggcgaa catcttgccg ctgcggccca
     7261 cgccggactg cacttcatcg agaatcaaca gaatgccgtg gcgatcgcac agctggcgca
     7321 agccgcccag aaacccatcg ggcggcacga cgtagccgct ctcgcattga atcggttcaa
     7381 ccaggatggc ggcgacttca ctggccggca cattgctgac gaataagacg ttctcaatgt
     7441 aattaagtac ggcctgacct tgatcgtcgc ccgcgaacag cggccggtaa ggattcggga
     7501 acggcacgtg ggtgacaccc ggcatggtcg cgaagccttg ctgctgcgtg cacttgctcg
     7561 cagtgaaagc cagcgaaccc atcgtgcgtc cgtggaaacc accgagaaag ccgatgaagc
     7621 gcgagcgtcc actgacataa cgcgccagtt ttagtgcgcc ttccaccgct tcggtgccgg
     7681 actgacagaa gaaactgagc actggctccc ccatgggcga cagcgcattg attttttcgc
     7741 cgagtgcgac ttgcctttca tgcacaaagt cagacgaaat gtgcaggaac tgatctgcac
     7801 aatccttgat cgcccgaact acctgcgggt gtgaatggcc ggtgctgcag acagcaatgc
     7861 cagcagcaaa atccaggaag cgattgccgt ctacgtccca aacttcaacg ccgcggccgt
     7921 gtgacattac gaacgggtac tcgcgacaat aagagggcga gaccacttcg gcgtcgcgcg
     7981 caagaagcgc acgcgccttc gggccgggca gttcggttcg caaggctcgg cggtcagaat
     8041 tgcgcatagt caaacctggg tttgcaacaa gcgatgcatt tcccgttgag caagtacgct
     8101 accagcgtgt gcgacttgcg ttcgcttttg cagatcacgg agaacgcaca gttcgcccaa
     8161 agttggcggt ggaacgacct ctactgtgtc agcaactgcc agaggccaac ccgtcgcggc
     8221 gcagattctg ccaacactga tgccgtcgta gctggcaaca agtgtgagct cctccgagcc
     8281 agaagccggc ttcagtatgc cgagatcggt gattactacc tgcggcccgg caccggaaat
     8341 ctcacgccgg gctcgttccc cacgtccggt taggaagcct gcgctggtgc aaaaatctag
     8401 gttcgcgaca aagctgcgcg gcgactgctt cagcacgatg aaaatctgct tggcatgctg
     8461 cgcaatttcg ggcgcgccac cggcacctgg cagccgcgtc ttcggtctac gataatcgcc
     8521 gatgaccgtc gtgttaaggt tgccaaagcg atcaatctgc gccgcgctga gaaagcccag
     8581 gtccatgcga cctgcctgca gccaatagga aaaaatttcc ggcaatgaca ccactgcggc
     8641 cgcagtttca gcaagctcgc catcgccgat cgatagaggc agtactttgg gctcggtgcc
     8701 aatgctgcca gattcgtaaa ctagcacgat gtccggagca tgagttaggc gcgcaagatt
     8761 cgctgccgcg gaaggtatcc cgatgcccac aaagcacaca cagcctggcg ggataagacg
     8821 cgccgccgcg atcgtcatca tctcatcggg agtgtatgaa ctacctgacg ttgaatgcgc
     8881 gctcatgcag cctccgccaa gctgtccaga aattccgcgt ggtcccgact acgaaccacg
     8941 tgtcgcgtga tccagtttgt gaacgtgtcg cggtcccggg cgatctcgtc ccatcgcctg
     9001 tagaaagcgt tatcccgtgc gtagtaaccg tgcgcgtaag atggataagc acccatcggg
     9061 accaccgcga ttgccgtgac tacccaatgc ggcaaaacga ttgcattcat cggcgcagac
     9121 agagactcga ccacttcttc aaccgtcacg atgagcgtgc gcgccgccaa ggccgcttct
     9181 ttctgcgcac cgacgatgcc gcggatgaga acgttgccct gacgatcagc ggcttgggcg
     9241 tgaagtaccg tgacatctgg attgatcgcc cgaacagcgg caaccgccgc gtctgtgtac
     9301 ggacaatgta cttctcgaat ttgaccgtta acctgtgaca gatcagttcc gacgtagccc
     9361 cgcaacattc cgaatggcaa ccgagcagcg ccggcggtgt aagccgccgc catccctgca
     9421 tgtgaatgtt cattgatctt cagtggctgc ggccagtcat tctcgaccgc atcgcgaagc
     9481 cggtgcagtg agccaacgcc gggattgcca ccccaggaaa acgtcagttc acgggcgcag
     9541 ccggcgccga tcagttgatc atagatcagg tccggagtca ttcggatcag atgcagctcg
     9601 cgttttttct gacgcaggat ctcgtgtccg gcggcgaatg gaatcaggtg agtgaaacct
     9661 tccagtgcga cactctgccc gtcgtcgacg tattcatcga tgacttcttg taacgtgccg
     9721 agctgcattg cctactctac tttggtttgc gattgttctc gttggtattg cgcaaggtag
     9781 tagaacgagg cgatgccctt ccccgaggag ccggatccct tccacccgcc gaagggctga
     9841 tatcctggcc aggcaccggt cgtggcaccc tgcggacgat taacataagt gacgcctgct
     9901 tctatgttgt cgaagaagta ggtggcatcg tcgtcgttcc cgtaacagcc ggccgttagg
     9961 ccgtagggcg tatcattggc caggcggatc gcttcctcgc gatcacgcac ccgacagagc
    10021 attgcgatag gcacgaacat ttcctccgca aagagtggat ggttagccgg cgcttccgct
    10081 aacgtgggcg cgacaaaata gccgtgtgcc agttcacgct ccgttagacg tcgaccgcca
    10141 cagaggatgc gagcgccatt ttcggcgagt tgtttactgt agcgctcgta cttatgcagc
    10201 gcatttgcgg tagttaccgg cccaagccag ttttcctgtc gcgtgggatc gcctatggtg
    10261 ataccagcaa ttttttctgt gagcttgtcg attaacgggt cggcgatttt ttcgtcaacg
    10321 tagatccgtg acagcgcgga acacttttgc ccgctcaagc catacgccga acgcacgatg
    10381 ccggcggcag cacggtccaa atcagcattg acagtcacaa tgcaggcgtt tttgccgccc
    10441 atctcg
//
LOCUS       DASTDG010000117        16202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_673919, whole genome shotgun sequence.
ACCESSION   DASTDG010000117 DASTDG010000000
VERSION     DASTDG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673919"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..479
                     /locus_tag="VFO30_03680"
     CDS             81..479
                     /locus_tag="VFO30_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418418.1"
                     /translation="MTTNATYLMQVLASAFLAILFLQSGIDKVVDRRGNLEWLRAHFA
                     KTPLAGFVPLLFAALTVLEIAAGALSAIGCLLLLLARNSTIAFYGALVSALSILALFF
                     GQRIARDYAGAAILVPYFLLALAAIYLLAR"
     gene            565..1305
                     /locus_tag="VFO30_03685"
     CDS             565..1305
                     /locus_tag="VFO30_03685"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9418419.1"
                     /translation="MFGRVIAFLYGLICYIIFLIAFLYAIGFVGNVVVPKNIDSGRGG
                     PLGPALIIDTVLLSLFALQHSIMARQWFKRAWTKIVPEPVERSTYVLLASLILLLLFW
                     QWRPMKAIVWDVHGPNGRAVLHGLYWLGWGLVFASTYLIDHFGLFGMKQVYNYLLGKR
                     DEPPPFKTPTLYKIVRHPLYLGFIIAFWSTPRMTAGHLFFALMTTAYILVAIQFEERD
                     LIRFYGDSYRKYRGQVSMLFPLRLRKRP"
     gene            complement(1385..2530)
                     /locus_tag="VFO30_03690"
     CDS             complement(1385..2530)
                     /locus_tag="VFO30_03690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9418420.1"
                     /translation="MFLSYLECTACGLRHDWTRLQNLCTACGKPLFAVVDLKAAGCAL
                     RRDKLATREKTLWRYRELLPLPDEAEPVSLGEGGTPLLRAKRFAQAVDLWIKDESLNP
                     TQSFKARGMSVAVSMAKYLGATKLAVPSAGNAGGALAAYAAAAGLEAHIFMPRDTPRA
                     NIVECRELGAHVDLVDGLITDCGAEIARRKEKEGWFDLSTLKEPYRIEGKKTLGYELA
                     EQLNWKLSDVILYPTGGGTGLIGMWKAFDEMEALGWIDNRRPRMFSVQASGCAPIVRA
                     FEAGEKFAAEFPNAQTIASGLRVPKAIGDFLILQILRASNGGVVAIDDQEIIRTVREV
                     GAKEGLFVAPEAAACFAALKKLCASAKIDTGERVMIFNTGSGIKYLD"
     gene            2677..4014
                     /locus_tag="VFO30_03695"
     CDS             2677..4014
                     /locus_tag="VFO30_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HET9418421.1"
                     /translation="MKEQTRPWYRPSLTIQIFIGLVIGGFIGWLRPDWGNAVYFLRDI
                     FINLIKSIIAPLVFSTIVVGIAGAGALKKVGRMGLKALVYFEIVTTAALFIGLAVVNF
                     AKPGAGVTLAASNTDVIKTIGQAHPKTFVETMVHAFPSSIVDAMVRGDVLQIVAFSVL
                     FALAISAVGETAKPIVRAMESLSQVMFKFTNYVMLFAPVGVGAAMAHTIGTQGPSVLV
                     NLGKLILSLYVALAIFVVLVFGLVIWITRVPLRQFVRAVREPAVLAFATTSSESALPK
                     AMQAMERFGVPRHIVGFVMPTGYSFNLDGSTLYLAVASVFVAQAAETTMGWHMSLAQQ
                     VTMMLSLMLTSKGVAGVPRASLVILLGTLNSFVPAGLGPIGVAIIFGVDELMDMARTC
                     VNVIGNCLATVVVARWERDLDDNRARMFGTTKEAELDLKSGDVAFADAAAHGD"
     gene            4033..4626
                     /locus_tag="VFO30_03700"
     CDS             4033..4626
                     /locus_tag="VFO30_03700"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET9418422.1"
                     /translation="MRTFFLFAPGAGAASSHPWMRRWKEQLSTQGEVKTFDYDYMREG
                     RKRPDPLPRLIERHRQALAEARAKTSAPIVLIGKSMGARVGCHLALQESVSGIICLGY
                     PLCGSGDQSKLRDKVLLDLRTPILFVQGTRDPLCPLDLLDAVRANMSVPNFLHVVEGG
                     DHSLLVRKKDLATETQEDVDQRILEAISHFASASNSS"
     gene            4700..6268
                     /locus_tag="VFO30_03705"
     CDS             4700..6268
                     /locus_tag="VFO30_03705"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET9418423.1"
                     /translation="METPLSPLEFARRTRTLYSERDAVVDGDLHLNYEKFFNRCDRWS
                     AALQTLGVKKGDRVAYIAPNTHAQLESFYAIPQIGAVLVPINYRLTADDFVYLITHSG
                     ARMVCADAGYLDAVDSIRSQIPDVLSFVALEGEKDGWLNYEALVAEAPRNFRRPTIKE
                     TDLLTINYTSGTTSRPKGVMITHRNAYINVIGTLLHHPITVADRYLWTLPMFHANGWT
                     FVWTVTAAGAAHICLRKVEAAAVFGKIKQESVTMLCAAPTVLISIAHAPEKLRREAPA
                     GLRIVTAGAPPAAATIERIEGDLGWTITQAYGLTETSPFITICESRPEHERLSCAERS
                     VIKAYQGVELLTSGELRVVNEEGKDVPRDGETIGEIVVRGNAVMKGYYNDPEATTEVI
                     RDGWFHTGDAAVLHPNGYAEIRDRLKDVIISGGENISSVEVEGVLLRHPAVQEVAIVG
                     LPHERWGEAPHAFVVLKAGASATESELRDFARNHLAHFKAPHTIRFLPELPKTATGKV
                     QKYILRGRPAISKQ"
     gene            complement(6377..8392)
                     /gene="speA"
                     /locus_tag="VFO30_03710"
     CDS             complement(6377..8392)
                     /gene="speA"
                     /locus_tag="VFO30_03710"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET9418424.1"
                     /translation="MFGALNHYAVRASPPGTRSTMKTEWDVESAIATYNVDRWGADYF
                     TINANGNVIARPLQKNGGSIDILEVVNEARSRGLGFPLVIRFQDLLRHRVESINRAFQ
                     NAITEFGYHNEYRGVFPIKVNQLREVIEEIIDAGRQFHFGLEAGSKPELVAALAMHQD
                     PESLVICNGYKDPAFVRIALLGRKLGKPVVIVVEKLEELEQTIRAAKEVGVDPHIGIR
                     LRLHSKGSGKWSPSGGENAKFGLDTTNLVAASHMLKEAGFAHCLKLVHFHVGSQVPDI
                     STIKRAVREAARYYAKLCKLGHELGYLDVGGGLGVDYDGSRSDFDSSANYSLQEYAND
                     VVWNIADVCDSESVAHPVIVSEGGRAIVAHHSVLVMEAFSSIEKTAPKLKVDATDKDH
                     RLVRDILDVKQRLKRGNRMESLHDIQQIKEEARQMFELGLLDLESKAKVETVYWQLAQ
                     QIVNMHRGLRYVPDEIKQLETSLGDQYICNFSVFQSLLDHWALGQLFPIMPIHRLTTQ
                     PDRNGMIVDITCDSDGKVSRFTDLQDVRDTLPLHRITPGEMYYLGVFMVGAYQDIMGD
                     LHNLFGRVTEVHVFLDPDEESGWYIEEVIEGSTIGEVLAMTQWDKVELLRLLKTQVDA
                     AIKGDRLKPNDAMKLLSDYERLLQEYTYLSLNGTKASAQPGNWLPLS"
     gene            8485..8955
                     /locus_tag="VFO30_03715"
     CDS             8485..8955
                     /locus_tag="VFO30_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9418425.1"
                     /translation="MNKTVIEVQVRAVLPTSGGCAVFLGNNDKVFIIYVDQTVGSAIT
                     MFMREISKERPLTHDLLGHLMTALGAKVERVIINDLKNATYYARMIIAAQNELQQRKI
                     IELDGRPSDCIAMATQQKAPIYVSQEVWDEVDDMSDVLRKMEEEGLKPDPEAEE"
     gene            complement(9012..10778)
                     /gene="ptsP"
                     /locus_tag="VFO30_03720"
     CDS             complement(9012..10778)
                     /gene="ptsP"
                     /locus_tag="VFO30_03720"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9418426.1"
                     /translation="MNGDARQEKRFHGAGVSAGVARGTIHVVRDDLDDVVRYKIEPSQ
                     IAHEIGRFEAALIQTRVQILEMQQKIAESIGAKDAGIFDAHLLVVEDRTLIDEVLRKL
                     QSELCNVEWVFQEVATNYIDTLSKIDDPYLRERALDIQDVMKRVIHNLQGKAPRPLFA
                     MTEPHILVAHNLTPSDTATMHKDWVLGIATDLGSRTSHTAILARSLNIPAVVGLHDVT
                     EKLETGQEVLVDGTNGLVIVDPTAETLAYYEQLESRRIKVVAKLRELRETKSTTRDGR
                     HIVLSANIELPDDVDAVAANGAEGIGLYRTEFLYLNRRTLPTEDEQYETYRKVADRVA
                     PNPLIIRTLDLGGDKLAPGTVEISDELNPFLGWRAIRLCLEHTDIFKTQLRAILRASV
                     IGNVKLMFPMISGLDELRRAMSILHECRDELRRAGVAFSEAIECGAMIEIPSAAISAD
                     ILAHEVDFFSIGTNDLIQYAIAVDRVNERIAHLYEPTHPAVVRLLKMIADAAHTNDIW
                     VGVCGEMAGDVALVPLLLGLGMDELSAAAILVPRVKRAVQSLTTTECRELVAQALQLQ
                     TASEILGRCLELANKRYGDLLG"
     gene            complement(10804..11115)
                     /locus_tag="VFO30_03725"
     CDS             complement(10804..11115)
                     /locus_tag="VFO30_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9418427.1"
                     /translation="MGLLSRRAGAGVRGEKVEKEITIVNRLGLHARPAAMFVRIASRY
                     RAEVWVSKEGEEVNGKSIMGLMMLAAGQGSKLRIRCEGPDAAKALEELEALINSRFNE
                     D"
     gene            complement(11135..12136)
                     /gene="hprK"
                     /locus_tag="VFO30_03730"
     CDS             complement(11135..12136)
                     /gene="hprK"
                     /locus_tag="VFO30_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET9418428.1"
                     /translation="MAERTGTQMITSTSDQKKSVPIVTVESFYTRHAEKLQMKLEGPR
                     VGFHRKIREPTINRPGLALSGFYTYFAEKRVQVLGAAEHSYLKSLSAKIRQQRFAELC
                     ARKIPCLVMSRGFHLASDLLEVTMKAKIAVFRTPMITMKFINAATIALEMDFSPTVIE
                     FGSMVDILGVGVLIRGASGIGKSECVLGLIERSYSLVADDVTRITSLEGRELMATAPE
                     LTRNHMEVRGIGIINVAAVFGIGSIRIEKRLDLVVTLQEWQDVEAVDRIGLDQEFYEI
                     LGLKVPHVTIPVRPGRDIARLIEVAAMDQKLKGLGQNSAVEFNTKLLNLMDTSSARA"
     gene            complement(12178..13359)
                     /locus_tag="VFO30_03735"
     CDS             complement(12178..13359)
                     /locus_tag="VFO30_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9418429.1"
                     /translation="MALALSASLISIWAGISVALVEILVGVIAGNFLGIHATTEWINF
                     LALLGSGVLTFLAGVEIDPQSLKANLRVSVFIGVLSFGIPFAVVWMFTQFVLGWPLHQ
                     AQIAGIALSTTSVAVVYAVMIEGRLSDTAMGKMILAACFITDFGTVLALGTLFANFNV
                     WLLVFLVTTSLVLWFMARWTQFIIDKLGATRVSEPEVKFIFFTLFFLGGLATRAKSEA
                     VLPAYLLGLVVAGVFARDKTLVHRMRSIAFATFTPFYFIKAGLYVSLPALWTALGLIA
                     VFLFLKIVTKVAGIFPLARLHYMNAREASYTTLLMATGLTFGTISALFGLQNKIIDQR
                     QYTILVTTIILSAFVPTIVAQKFFQPSIEAMHAWGRLYQRKISAFRVMSVEDVNDEEN
                     G"
     regulatory      complement(13413..13485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(13532..14863)
                     /gene="gltX"
                     /locus_tag="VFO30_03740"
     CDS             complement(13532..14863)
                     /gene="gltX"
                     /locus_tag="VFO30_03740"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9418430.1"
                     /translation="MASEIRVRFAPSPTGYLHIGGARTALFNWLFARHHGGKFVLRIE
                     DTDIKRNTEEAAAAIYEGLEWLGLNWDEGPHVGGDFGPYFQSQRTEIYERYLKKLQDG
                     GHIFEDQGALRFRSPREHVVVDDLVCGKIDFDLTNPGTHPDMTIRRPDGSWIFHFVNV
                     IDEIEMKISHVIRGEDHLSNTPKHIEIFRALGATPPHYAHIPLILNRDGSKMSKRDEG
                     ARVATYIEQGYAPEAVRNYLCLLGWSPKDNREKIDIEEVVRLFELEKLNRRNAAFDLD
                     KCFWLNGQYVAQMSLDRFVELARPFMEKANIDISDEKYLREVLAIVKEKIKLFKDVPE
                     WTAYFFTETYEFDPAAIEKVFGQPEAATRLSALRDEFGKIDKWNVGEIESRLKTLAQR
                     LGCKTGDLVHPARVAVSGRSVGPSLYHMLEVMGKERVLARFERMISQLGVE"
     gene            complement(14938..15972)
                     /gene="tilS"
                     /locus_tag="VFO30_03745"
     CDS             complement(14938..15972)
                     /gene="tilS"
                     /locus_tag="VFO30_03745"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9418431.1"
                     /translation="MNRIQFSNGILRRFPARERYLVGVSGGCDSVALLHLLIDFGYKN
                     LVVCHLNHRLRGRSADADARFVQKLVAFYSSKIVGQAHRPPNRKDGKRSARPTTPTAH
                     VDLEMGTVDVRALAAKRKMSTETAAREARYAFFARAARRRRCQTIFLAHHADDLVETF
                     LLNLFRGAGMTGLAAMRELSVRQISDIELTIVRPLLSVWRTQIDDYARKHRLKFREDA
                     SNKDLALARNRIRHRVVPYLERTLGREIRQSIWRAANIAAHEEAWIDRRMPESSEADL
                     AVAKLRELPVALQRRAIVNWLRSRDIKNVGFDVVEAVRLLLEPDVRVAKINLPQDRHV
                     RRRAGKIFVE"
BASE COUNT         3398 a         4686 c         4595 g         3523 t
ORIGIN      
        1 gccagaaatt tttgcgctgc aagacaagat cgacaatcgc gcggcgcttt gaataaattt
       61 tgatgccaca cgcctgcgca atgacgacaa acgcaactta cctgatgcag gtcctagcgt
      121 cggcttttct ggccatcctt tttctgcaat ccggcatcga caaagttgtc gaccgccgcg
      181 ggaacctcga gtggttgcga gcgcattttg ccaaaactcc gcttgccgga tttgtgccat
      241 tactgtttgc cgcactgacg gtgctcgaaa tcgccgctgg agcgctaagc gcgatcggct
      301 gcctgctcct gcttttggcg cgcaattcaa cgatcgcgtt ttacggcgcg cttgtctccg
      361 cattgtcgat cttagcgctg ttcttcggcc agcgaatagc cagggactac gctggcgcgg
      421 cgatcctcgt cccgtatttt cttctcgcgc ttgctgcgat ctaccttttg gcgcgttgaa
      481 acagttttcg acttgccagt aactttaagg ttgtcactat gcttttggcg ccgcaatact
      541 gacagagaac aacaagcgac tcatatgttc ggtcgagtca tcgcgttcct ttacgggctg
      601 atttgctaca tcatcttcct cattgcgttt ttgtatgcca tcggattcgt tggcaacgtt
      661 gtcgttccga aaaatattga ttcggggcga ggcggcccgc tcgggccggc actcatcatt
      721 gacacggtgt tgctgagctt gttcgctctt cagcacagca tcatggcgcg ccaatggttc
      781 aaacgcgcgt ggacgaaaat cgtgccggag ccggttgaac ggagcaccta tgtcttattg
      841 gccagcctca ttctcttgct gctcttttgg cagtggcggc cgatgaaagc gattgtctgg
      901 gacgtccatg gtccgaatgg ccgcgccgtg ttgcacggtt tgtattggct cgggtggggc
      961 ctggtttttg catccactta tctcatcgac cacttcggcc ttttcgggat gaagcaagtt
     1021 tacaactatt tgcttggcaa gcgcgatgaa ccgcccccct tcaagacccc gacgctgtac
     1081 aaaatcgtgc ggcatccact ttatttggga ttcatcattg cgttttggag cacgccgcgc
     1141 atgacggcgg gacatttgtt cttcgcccta atgactactg cttacatcct cgtcgccatc
     1201 cagttcgagg aacgcgatct gattcgtttc tacggcgata gctaccgaaa gtatcgcggg
     1261 caggtgtcga tgttgttccc gctgcgcctc cgcaaacggc cctaagctcg cgacttgcca
     1321 ggcccagcac taagacggcc gcgaaggacg aggcgaacgg ccaccgccta acgagtcgtc
     1381 gaattcagtc gagatactta atccccgagc ccgtgttgaa gatcatcacc cgctcgcccg
     1441 tgtcgatctt ggcggatgca caaagcttct tcaacgcggc aaagcatgcc gcggcttcgg
     1501 gagcgacgaa cagcccttct ttggcgccga cctcccggac cgtgcggatg atttcctggt
     1561 catcgattgc aacgacaccg ccgttcgatg cgcggagaat ctgcagaatg aggaaatcgc
     1621 cgatcgcttt gggcacgcgc agaccggagg cgatcgtttg cgcattggga aactcggctg
     1681 cgaatttctc gccggcttca aatgcgcgga cgatcggcgc gcagccgctc gcctgcaccg
     1741 aaaacatgcg cggccggcga ttgtcgatcc aaccgagcgc ttccatttca tcgaacgctt
     1801 tccacattcc gatgagaccg gtgccgccgc cggttggata aagaatcaca tccgataact
     1861 tccagttgag ttgctcggcc agctcgtagc cgagcgtttt cttgccttcg atccgatacg
     1921 gctccttcag cgtcgacagg tcaaaccagc cttccttctc tttgcgccgc gcgatctccg
     1981 cgccgcaatc ggtgatcagc ccatcgacaa gatcgacatg ggcgccgagc tcacggcact
     2041 cgacgatgtt cgcgcgcggc gtgtcgcgcg gcataaaaat gtgcgcttcg agtccagcag
     2101 ccgcagcata agcggccagt gcaccgccgg catttccggc gcttggcacg gcgagtttag
     2161 tcgcgccgag atattttgcc atcgaaaccg cgacggacat gccgcgcgct ttaaaacttt
     2221 gcgtcggatt gagcgactcg tctttgatcc aaagatcgac agcttgcgcg aaacgtttcg
     2281 cgcgcagcag cggggtgccg ccttcgccta acgacaccgg ctccgcctcg tccggcagcg
     2341 gtagtagttc ccggtaacgc cacaacgttt tctcgcgcgt cgccaatttg tcgcggcgaa
     2401 gggcgcaacc ggcggcctta agatcgacaa ctgcaaacag tggtttgccg caggcggtgc
     2461 aaagattttg cagacgcgtc caatcgtgcc gcaggccgca cgctgtgcac tcgaggtaac
     2521 tcagaaacac tccgcaattg aacctctgcg cgcgaaattg gaaaggtgga tcgcgccttc
     2581 cgccgcgcga tgctaaaaag atgcggctta tccgcctaat tgacatcgag cgcggagcgc
     2641 tcgatccacc tcgctcccta tgctaatcac gacagagtga aggaacaaac gcgaccgtgg
     2701 taccgaccgt cgctcacaat acaaattttc atcggcctcg tcatcggcgg gttcatcggc
     2761 tggctgcggc ctgactgggg caacgccgtt tattttctgc gcgatatctt tattaacctg
     2821 atcaaatcga tcatcgcgcc gctcgttttc tcgaccatcg tggtgggcat cgccggcgca
     2881 ggcgcgttga agaaggtcgg gcgcatgggc ctgaaggcgc ttgtctactt cgagatcgtc
     2941 acgacggcgg cgctcttcat cggtttagcg gtagtgaatt tcgccaaacc cggagcgggc
     3001 gtgacccttg cggccagcaa caccgatgta attaagacca tcggccaggc gcacccgaag
     3061 acattcgtcg aaacaatggt gcatgcattc ccatcgagca tcgtcgacgc gatggtgcgc
     3121 ggcgatgtgt tgcaaatcgt cgccttctcg gtgctgttcg cgctcgctat ttcggccgtc
     3181 ggcgaaaccg ccaaaccgat tgtgcgcgcg atggagagtc tgtcgcaggt gatgttcaag
     3241 ttcaccaatt acgtgatgct cttcgcgccc gtcggcgtcg gggccgcgat ggcgcacacg
     3301 atcggcacgc aaggaccaag cgtgctcgtc aatctcggta agctgatcct ctcgctctac
     3361 gtcgcacttg ccatttttgt tgtgctggta ttcggactcg tgatttggat aacgcgagtt
     3421 cctttgcggc aattcgtgcg cgcggtgcgc gagccggcgg tgctcgcatt tgccacgacg
     3481 agcagcgaat cggcgttacc aaaagcgatg caggcgatgg aacgcttcgg ggtgccgcga
     3541 cacatcgtcg gcttcgtcat gccaaccggc tactcgttca acctcgatgg ctcgacgctc
     3601 tatcttgccg tggcttcggt cttcgtcgcg caggcggccg agacgaccat gggctggcac
     3661 atgtcgttag cccagcaggt cacgatgatg ctgagcttaa tgctaacatc aaaaggcgta
     3721 gctggggtgc cgcgcgcttc gctcgtgatt ctgcttggca ccttaaattc gtttgtgccg
     3781 gcaggactag ggccaatcgg cgttgcgatc atttttggcg tggacgagtt aatggacatg
     3841 gcgcgcactt gcgtaaacgt gattggcaac tgcctggcga ccgtggtcgt cgcgcgctgg
     3901 gaacgcgact tggacgataa ccgtgcacgc atgttcggta cgacgaagga agcggagctc
     3961 gatttgaaat ctggtgacgt cgccttcgcg gacgcggcag cacacggcga ttaagatcca
     4021 cattaggcaa tcgtgcggac gttctttctc tttgcgccgg gtgcgggcgc ggcatcatct
     4081 catccgtgga tgcgtcgctg gaaagagcag ctgagcacgc agggagaagt taagacgttc
     4141 gattacgact acatgcgcga ggggcggaaa cgccccgatc ctctcccgcg actgattgaa
     4201 agacatcgtc aagctctagc cgaggcgcgg gcgaaaacgt ccgcgccaat cgttctcatc
     4261 gggaagagca tgggcgcgcg agtcgggtgt catctcgcgc tgcaggaatc ggtaagtgga
     4321 atcatctgcc tcggttaccc gctttgcggc agcggcgatc aatcaaagct gcgcgacaag
     4381 gttttgctcg atctgcgaac gccgattctg tttgtccagg gcacacgcga cccgctttgt
     4441 ccgctcgatt tgctcgatgc ggttcgcgca aacatgagcg tgccaaattt ccttcacgtc
     4501 gttgaaggcg gcgatcattc gctactcgtc agaaaaaaag atctcgcaac cgaaacgcaa
     4561 gaggatgtcg atcaaagaat ccttgaagca ataagccact ttgcttcggc atcgaacagt
     4621 tcgtaatgat ctgcgaaaat cgaattgaac cccgttttgg gacggatgca cactgaacgc
     4681 aacgggaggt cgaagtgtta tggaaactcc gttgagtccc ctcgaattcg cgcgtcgcac
     4741 tcgtacgctc tactcggagc gagacgccgt ggtggatggc gatttgcacc tgaattatga
     4801 gaaatttttc aaccgatgtg atcgctggtc agcggcattg cagacactgg gcgtgaaaaa
     4861 aggtgaccgc gtcgcttaca tcgcacccaa cactcacgcg caattggaat cgttttacgc
     4921 catcccgcag atcggtgcag tgctcgtacc gatcaactat cggctaacgg cagacgattt
     4981 cgtttatctg attactcaca gcggcgcgcg gatggtgtgc gcagacgcgg gttatctgga
     5041 cgcggtcgac agtattcgat cacaaatccc tgatgttttg tcctttgtcg cgttggaagg
     5101 tgagaaggac ggctggttga attatgaggc gctcgtggcc gaagcgccgc ggaacttccg
     5161 gcgaccgacg atcaaggaaa ccgatctgct cacgatcaat tataccagcg gcactacttc
     5221 aaggccgaag ggagtgatga tcacgcatcg aaacgcttac atcaatgtga tagggacgct
     5281 gcttcatcat ccgataacgg tggcggatcg ttatctctgg actttgccga tgtttcacgc
     5341 caacggctgg accttcgtct ggacggtaac ggcagcgggc gcggcgcata tttgtttacg
     5401 aaaagtcgaa gccgccgctg tgttcgggaa aattaaacaa gagtccgtca ccatgctttg
     5461 cgcggcgccg accgtgctta tcagcattgc tcatgcgccg gagaaactca gacgtgaagc
     5521 gcccgccggc cttcgcatcg tcacggccgg tgcgccgcca gcggcggcca ccattgagcg
     5581 tatcgaggga gacctcggct ggacgattac tcaagcctat ggcctgactg agacctcccc
     5641 ctttatcacg atttgcgaat cgcgacctga acacgagcgg ctttcgtgtg cagagcggtc
     5701 cgtaatcaag gcgtatcagg gtgtcgagct acttacgtca ggagaacttc gtgtggtcaa
     5761 cgaagaagga aaggacgttc cgcgcgatgg tgaaacgatc ggcgaaattg ttgtccgcgg
     5821 gaatgcggtt atgaaaggct attacaatga tcccgaggcc acgactgaag taatacgcga
     5881 cggttggttt cataccggag atgccgccgt cctccatccc aacggctacg ctgagattcg
     5941 agatcgccta aaagatgtga tcatcagcgg cggcgaaaat atctcatccg tagaagtgga
     6001 aggcgtattg ctgcgacacc cggccgtcca agaggtcgca attgtcgggc ttccacacga
     6061 gcgctgggga gaagcgccgc atgcgtttgt ggtgttgaaa gcaggagcta gcgccactga
     6121 aagtgaactg cgcgattttg cacgcaacca tctcgcgcat ttcaaggctc cacataccat
     6181 ccgatttctc ccggaacttc cgaaaacggc gactggcaaa gttcaaaagt atatactccg
     6241 cggcagaccg gcgatttcca aacagtaatg gtgctgtggg aacgccctct agtctgttgg
     6301 gagacaacag tacgcggcgc gccagctgtg tacaatcagc gattttcatt tcgcaagatc
     6361 gacaatcgcc ggtcgatcaa ctcaacggca gccagtttcc cggttgcgcc gacgctttcg
     6421 tgccgttgag ggagagatag gtgtattcct ggagcaggcg ctcgtaatcg gagagaagtt
     6481 tcatcgcgtc attcggtttc aggcggtcgc ccttgatcgc ggcgtcgacc tgggttttga
     6541 gcaagcgcag cagctcgact ttgtcccact gcgtcatggc caggacttcg ccaattgtgc
     6601 tcccttcgat gacttcctcg atgtaccagc cggattcttc atcgggatcg agaaagacgt
     6661 gcacctcggt gacgcggccg aacaagttgt gcaggtcgcc catgatgtcc tgataagcgc
     6721 cgaccataaa cactccgagg tagtacattt cgccgggcgt aatccgatgc agcggaagcg
     6781 tgtcgcgcac gtcctgtaga tcggtaaatc tggagacttt accgtcggaa tcgcaggtga
     6841 tgtcgacgat cataccgttg cggtccggct gagtcgttag gcgatggatc ggcatgatcg
     6901 gaaacagttg gccgagcgcc caatgatcga gcagcgattg aaagacggag aagttgcaaa
     6961 tgtattggtc gcctaacgaa gtctcgagct gtttgatttc atcgggcaca tagcgcaggc
     7021 cgcggtgcat gttaacgatc tgttgcgcga gttgccagta gacggtttcg acctttgcct
     7081 tcgattcgag gtcgagcaag ccgagctcga acatctgccg cgcttcctcc ttgatctgct
     7141 ggatatcgtg caagctctcc atgcgattgc cacgtttgag ccgctgtttc acgtcgagaa
     7201 tgtcccgcac aagcctgtga tctttgtcgg tggcatccac tttcaatttc ggcgcggttt
     7261 tctcgatcga actgaatgct tccatcacca gcacggaatg gtgcgccacg atcgcgcggc
     7321 cgccttcgct cacaatcacc gggtgcgcga cactttcgga gtcgcacaca tccgcgatgt
     7381 tccagacgac gtcattcgcg tattcctgaa gcgagtagtt agccgagcta tcgaaatcgc
     7441 tgcgcgaccc gtcataatcg acacccagcc cgccgccaac gtcgagataa ccgagctcgt
     7501 ggccaagttt gcagagcttg gcgtagtagc gcgcggcttc gcgcacggcg cgcttgatcg
     7561 tggaaatgtc gggcacctgc gagccgacat ggaaatggac gagcttcaaa cagtgcgcga
     7621 agcccgcttc cttcaacatg tgactggccg cgaccagatt ggtcgtatcc agcccgaact
     7681 tcgcgttctc accaccgctc ggtgaccatt tacccgagcc tttgctgtgc agccgcaggc
     7741 gaataccgat gtgcggatca acgccgactt ccttcgccgc gcgaattgtt tgctccagct
     7801 cctcgagttt ttcaacaacg atgacgaccg gtttgccgag cttgcgcccg aggagcgcga
     7861 tccgaacaaa tgcgggatcc ttatagccat tgcaaatgac gagactctcc gggtcctgat
     7921 gcatggcgag cgccgccacc aattccggtt tgctgccggc ctccagaccg aaatgaaact
     7981 gccggccggc atcgatgatt tcctcgatca cttcccgcag ctgattgact ttgatcggga
     8041 aaacgccgcg atactcgttg tgataaccga attcggtgat cgcgttttga aaggcgcggt
     8101 tgatcgattc gacccggtgt cggagcaagt cctgaaagcg gatgacaaga ggaaagccga
     8161 ggccgcgcga ccgcgcttcg ttcaccactt ctaaaatgtc gatcgagccg ccgtttttct
     8221 ggagcggacg cgcgatgacg ttgccgttcg cgttgatcgt gaaatagtcc gcaccccagc
     8281 ggtcgacgtt gtaagtggcg atggctgact cgacgtccca ctcagtcttc attgtcgatc
     8341 ttgtgcccgg cggtgaagcg cgaaccgcat aatgattcag tgcgccgaac atgcaaggct
     8401 tgtgccaaaa aattcgcgcg cagttattca caggcgggcg gtccgcttgt ttttcggcgt
     8461 cgcgcagata acgttgcggc cgggatgaat aaaacggtga tcgaggtgca agtgcgcgcc
     8521 gtcctgccaa cgagtggcgg atgcgccgtt tttctgggta acaacgacaa agtcttcatt
     8581 atctatgtcg atcaaacggt gggcagcgcg atcaccatgt tcatgcgcga gatcagcaag
     8641 gagcgcccgc ttacgcacga tctgctcggc cacctgatga cagcgctcgg cgcaaaagtg
     8701 gaacgtgtga tcatcaacga cctgaaaaac gcaacctact acgcgcggat gatcatcgcc
     8761 gcccaaaacg aattgcagca gcgcaaaatc atcgagctcg atggccgccc gagcgattgc
     8821 atcgccatgg cgacgcagca aaaagcgccg atctacgtca gccaggaagt ctgggacgaa
     8881 gtcgacgaca tgagcgacgt gctgcgcaag atggaagagg aaggcctcaa gcctgatccc
     8941 gaagcggaag agtagcgcaa gtctccggct tgcgatgtaa agtaagtcgc aagcgagacg
     9001 cttgcgctac tttagcctaa cagatcgccg taacgcttat ttgccagctc caggcagcgg
     9061 cccaggatct cggaggcggt ttggagctgc aacgcttgag cgacaagttc gcggcattcg
     9121 gtggtggtga ggctttgcac agcgcgtttg actcgcggta cgaggatggc ggccgcgctg
     9181 agctcatcca tgccaaggcc gagcaagagc ggaacaagcg cgacatcacc tgccatttcg
     9241 ccgcacacgc ccacccagat gtcgttcgtg tgcgcagcgt cggcgatcat cttgagcaaa
     9301 cgcaccacgg cgggatgcgt cggctcataa agatgggcga tccgctcgtt cacgcggtcg
     9361 accgcgatcg cgtattgaat caggtcgttc gtcccgatgc tgaagaaatc gacttcgtgg
     9421 gcgaggatgt cggcggaaat ggcggcgctc gggatttcga tcattgcgcc gcattcaatc
     9481 gcttcgctga aagcgactcc ggctcggcgc agctcatcgc gacattcatg caagatcgac
     9541 atcgcgcggc gcaattcgtc caagcctgag atcattggga acatgagctt gacgtttccg
     9601 atgacgctgg cgcgaagaat ggcgcgcaat tgtgttttga agatgtccgt gtgctcgagg
     9661 caaagtcgaa tcgcgcgcca gccaagaaaa gggttcagct cgtcgctgat ctcgactgtg
     9721 ccgggcgcga gtttatcgcc gccaagatcc aatgtccgga tgatgagcgg gttcggcgcc
     9781 acgcgatcgg ccaccttgcg ataggtctcg tactgctcgt cttcagtcgg caacgttctg
     9841 cggttcaaat aaaggaattc ggtgcgataa aggccgatgc cttcagcgcc attggccgcc
     9901 accgcgtcga catcgtcggg caattcgatg tttgccgaga gaacaatgtg gcgcccatcg
     9961 cgcgtcgtgg attttgtctc gcgaagttca cgcagtttcg cgacaacttt gatgcggcgc
    10021 gactcgagct gctcgtaata cgcgagcgtc tccgcggttg gatcgacaat caccaaaccg
    10081 ttggtgccgt cgaccagcac ctcctggccg gtctcgagct tttccgtcac gtcgtgcaaa
    10141 ccgactacgg ctggaatgtt cagcgagcgc gcgagaattg ccgtgtgcga agtgcggctg
    10201 cccaagtcgg tggcgatgcc gagaacccaa tctttgtgca tggttgcggt gtccgagggc
    10261 gtgagattgt gcgcgaccaa aatgtgcggc tcggtcatgg caaacagcgg tcggggcgct
    10321 ttgccctgca aattgtggat gacgcgcttc atcacgtcct gaatgtccag ggcgcgctcg
    10381 cgcagatagg ggtcgtcaat tttgctgagg gtgtcgatgt aattggtcgc gacttcctgg
    10441 aagacccatt ccacgttgca gagctcgctc tgcagtttgc gcagcacctc atcgatcagg
    10501 gtgcggtctt cgaccacgag caggtgcgcg tcgaaaatac cggcgtcctt cgcgccgatc
    10561 gattcggcga tcttttgctg catctcgagg atttgcaccc gcgtctggat gagcgcggcc
    10621 tcaaaacgac cgatctcgtg ggcgatttgc gacggctcga tcttatagcg gacaacatcg
    10681 tcgagatcgt cacgcaccac gtgaattgtc ccgcgggcga cgccggctga gacacctgca
    10741 ccgtgaaatc gcttttcctg ccgcgcgtca ccgttcatcc cgaccgcgag agtagccccg
    10801 aaatcaatct tcgttaaagc gcgaattgat cagtgcctcg agctcctcca atgccttcgc
    10861 cgcgtccggt ccctcgcagc gaatccgcag cttcgagcct tgtccggcgg ccagcatcat
    10921 caggcccatg atgcttttgc cgttgacctc ttcgccctct ttcgacaccc acacttccgc
    10981 gcgataacgg ctggcaatgc gcacgaacat cgccgccggt cgggcatgaa gcccgaggcg
    11041 attcacaatc gtaatttcct tctcaacttt ttctccgcgc acgccggcgc cggctcggcg
    11101 actcaacaat cccatgttgt cgatcttgtc tgagtcatgc tcgtgccgaa cttgtgtcca
    11161 ttaaattgag cagcttggtg ttgaactcga ccgcgctgtt ctggcccaag cccttcaatt
    11221 tttggtccat cgccgcaact tcgatcaacc gcgcaatatc gcggcccggg cgaaccggga
    11281 ttgtgacgtg cggtactttc agcccaagaa tttcgtaaaa ttcctggtcg agcccgatgc
    11341 ggtcaactgc ttcgacgtcc tgccattcct gcaaggtcac caccagatcg aggcgtttct
    11401 cgatgcgaat gctcccgatg ccgaaaaccg cggccacatt aatgatcccg atcccgcgca
    11461 cttccatgtg attgcgcgtc aactccggcg cggtcgccat taattcacgg ccttccagtg
    11521 acgtgatgcg cgtcacgtca tcggccacga ggctgtagct gcgctcgatc agaccgagca
    11581 cgcattcgct cttgccaatg ccgctcgcgc cgcgaatcaa aactccgact ccaagaatat
    11641 cgaccatgct gccgaattcg atcaccgtcg gcgagaaatc catctccagc gcgattgtcg
    11701 ccgcgttgat gaacttcatt gtgatcatcg gcgtacgaaa caccgcgatc ttcgccttca
    11761 tggtcacttc cagcagatcg gacgcgagat gaaacccgcg cgacatgacc agacacggaa
    11821 tttttcttgc gcagagctcg gcaaagcgct gctgccgaat ttttgccgac aaactcttca
    11881 agtaggaatg ctccgccgcg ccgagcacct gcactctttt ctcggcgaaa taagtgtaga
    11941 aacccgacag cgccaggccg ggtcgattga tcgtgggctc gcgaattttg cgatgaaaac
    12001 cgacgcgcgg cccttccagt ttcatctgca gcttttcggc gtgacgcgtg tagaagctct
    12061 ccaccgtcac gatcggcacg ctcttctttt gatcgcttgt cgatgtgatc atttgcgtgc
    12121 cggtgcgctc cgccatggcg cctaactgtt agcgcgaatg ctccgcgatt tccaatctca
    12181 gccgttctcc tcgtcgttta cgtcctccac gctcatgacc cgaaaggcag aaatcttccg
    12241 ctggtaaagg cgtccccacg cgtgcatcgc ttcgatgctc ggctggaaaa acttttgcgc
    12301 gacgattgtc ggcacgaatg cgctcaggat tatggtcgtc accaggatcg tgtattggcg
    12361 ctggtcgatg atcttgttct ggagaccaaa caacgctgag attgtgccga acgtaaggcc
    12421 ggtcgccatc aacaatgtcg tgtagctggc ctcgcgcgcg ttcatgtaat gcaggcgcgc
    12481 caatggaaag atacccgcca ccttcgtcac tattttgaga aataagaaca cggcgatgag
    12541 gccgagagcc gtccacaacg ccggcaatga cacatacagc cccgctttga tgaaataaaa
    12601 aggcgtgaac gtggcgaacg cgatgctgcg catgcgatgg accaacgttt tatcgcgggc
    12661 aaatacaccg gcgactacga gtccgagcaa atacgcaggc agaaccgctt cgctctttgc
    12721 tcttgtagcg agaccgccaa ggaaaaatag cgtgaagaag atgaacttca cctcgggctc
    12781 gctcacccgc gtcgcgccca gtttgtcgat gatgaactgc gtccagcggg ccatgaacca
    12841 gagcacaagc gacgtcgtca caaggaacac gagcagccag acattgaaat tggcaaacag
    12901 agtgccgagc gcgagcaccg ttccgaaatc ggtaatgaag cacgcagcca aaatcatttt
    12961 gcccatggcc gtgtcgctca gcctgccctc gatcatgacc gcgtagacga ccgccaccga
    13021 cgtggtcgaa agcgcgatgc cagcgatctg cgcctgatgc aagggccagc caaggacgaa
    13081 ctgtgtgaac atccacacga ccgcgaaagg aattccgaac gacaaaacgc cgatgaatac
    13141 gctcacccgc aggttcgctt ttaacgactg cggatcgatc tccacacccg cgagaaaggt
    13201 gagcacgccg ctgccgagca gcgcgagaaa attgatccac tctgtcgtcg cgtggatgcc
    13261 gagaaaattg ccggcgatca cgccgacgag tatttcaacc agcgcgacgg aaataccggc
    13321 ccagatcgag atcaggctcg ccgacaaagc gagtgccatc caaattgcag caacaaacca
    13381 gacgttttgc gtcataaact cctacctttc cgctgatggt aggagtcatt agctcacgca
    13441 gtttccctcg cgctgaggga ggcaaactcc attgcctgct gaaagctaac ggtgcgtctg
    13501 gttcgatcaa caaaaagttg gtcgaacgat tctattccac gcctaattgc gaaatcatcc
    13561 gctcgaaacg cgcgagcacg cgttctttgc ccatcacttc gagcatgtgg taaaggctcg
    13621 ggccgacgct gcggccgctc accgctacgc gcgcgggatg aacgaggtcg ccggttttgc
    13681 aaccgagcct ttgcgcgagt gtctttaaac gcgattcgat ctcaccgacg ttccacttgt
    13741 cgatcttacc aaattcatca cgcagcgcgc tcaagcgtgt tgccgcttca ggttggccaa
    13801 acactttttc gattgccgcg ggatcgaact cgtatgtctc ggtaaaaaaa taagccgtcc
    13861 attccgggac atctttgaaa agcttgatct tctccttcac gatcgccagc acctcgcgca
    13921 ggtatttttc gtcggagatg tcgatgttcg ctttctccat gaacggccgc gccagttcca
    13981 caaagcgatc gagcgacatc tgcgcgacgt actggccgtt gagccaaaaa catttgtcga
    14041 gatcgaacgc ggcgttgcgg cggttaagct tctccagctc gaatagcctg acgacttctt
    14101 cgatgtcgat cttttcccgg ttgtccttgg gcgaccaacc gagcaggcaa agataattgc
    14161 gcactgcctc cggcgcgtag ccttgttcga tgtaagtggc gacacgcgcg ccctcgtcgc
    14221 gcttgctcat tttcgatccg tcgcggttca gaatcagtgg gatgtgcgcg taatgcggcg
    14281 gcgttgcgcc gagtgcgcgg aaaatctcga tgtgcttggg cgtattcgag agatggtctt
    14341 cgccgcggat gacgtgcgag atcttcattt cgatctcgtc gatcacgttg acgaaatgaa
    14401 aaatccacga gccgtccggg cggcggatgg tcatgtccgg atgcgtgccg ggattcgtca
    14461 ggtcaaagtc gatcttgccg cagacgagat catccaccac gacgtgctcg cgcggcgaac
    14521 ggaaccgcag cgcgccctgg tcctcgaaga tgtgaccgcc gtcctgaagt ttcttgaggt
    14581 agcgctcgta aatctcggtg cgctgacttt gaaagtaggg gccaaaatcg ccgccgacgt
    14641 gcgggccttc gtcccagttg agcccgagcc attccaagcc ttcgtaaatc gcagccgccg
    14701 cttcttcggt gttccgtttg atgtcggtat cttcgatccg cagaacgaac ttgccgccgt
    14761 gatgccgcgc gaatagccag ttgaatagcg ctgtgcgcgc cccgccaatg tgcaggtagc
    14821 cagtcggtga gggcgcaaat cggacgcgaa tttcagaggc cattgggaag cactgtagcg
    14881 gcgctctatg agcgtcgcat tagttttgcg acggtcatag accgccgcta cagaagatta
    14941 ctccacaaaa atcttccctg cgcggcggcg cacatgtcga tcttgcggga ggttgatttt
    15001 cgcgacgcgc acgtccggct cgagaagcaa gcgcacggct tcaacgacgt cgaaaccgac
    15061 atttttgatg tcgcgcgagc gcaaccagtt cacaatcgca cggcgttgca gcgcaacggg
    15121 caattcgcgt agttttgcaa ccgcaagatc ggcctcactg gactcgggca ttcgccgatc
    15181 gatccaagct tcctcgtgcg cggcaatatt cgccgcgcgc cagatgcttt gccgaatctc
    15241 gcgaccgagg gttctttcca agtacggaac gacgcgatgg cgaatccggt tccgcgccaa
    15301 cgcgagatct ttgttcgatg catcttcacg gaatttgagc cggtgcttgc gcgcgtagtc
    15361 gtcaatctga gttcgccaaa cgctcaataa cggccgcacg atggtaagct cgatgtcact
    15421 gatttggcgc accgacagtt cgcgcatggc ggcgagtccg gtcatgccgg cgccgcggaa
    15481 aagattgagc agaaaagttt cgaccagatc gtcggcatga tgggccagaa aaattgtttg
    15541 gcagcggcga cgtcttgcgg cccgagcaaa gaatgcgtag cgcgcctcgc gcgcggccgt
    15601 ttccgtggac atcttccttt ttgcggccag cgcgcgaacg tcgactgttc ccatctcaag
    15661 atcgacatgc gccgtcggag ttgtagggcg agcgcttcgc ttgccgtctt tgcggttcgg
    15721 cgggcgatgc gcctgcccta caattttgct gctgtaaaaa gcgacgagtt tttggacgaa
    15781 acgcgcatcc gcgtcggcgg agcggccgcg gagtcgatga ttcaagtggc agaccaccag
    15841 atttttgtaa ccaaaatcga tcagcagatg cagcaacgcg accgagtcgc atccgccgga
    15901 aacgccgacc aagtagcgct cgcgcgcggg aaaccggcgc aaaattccgt tgctaaactg
    15961 aatccgattc atcgggtcgg cgcatcgaat gtctgatgcc aacgatcaac gttccatgcc
    16021 gcgtcgcctt agacaaaggc ggagcgcggc ggcgtcttca caaagaaagg acattcatga
    16081 acaaccgaac aacttactca ttgcttctcc gctccgagga aaagagccgg gacatgctgg
    16141 aaacgacggt ctacgcgcta tgcgtcctga gcgcagtctt tgccatctgg caatttgcgc
    16201 aa
//
LOCUS       DASTDG010000118         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1311713, whole genome shotgun sequence.
ACCESSION   DASTDG010000118 DASTDG010000000
VERSION     DASTDG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311713"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFO30_03750"
     CDS             <1..1096
                     /locus_tag="VFO30_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9418432.1"
                     /translation="EPLTQTTLKNLQRATTLLPKTAPSAADVQQTLIAFDARRDSSKP
                     PGEYDFALRPNESFRPVPMLSVSDLERMVSSPAIIWMKHYLGVEAPDDDANPWSAATG
                     KWVHDWLASIAETPAGKIFAPFPSSTKIDKCIRLAADERRAALQRLCELLGKVVPDWW
                     NSGWLNARYLARHLGSKIATATGWDWMAAEFPAGRGDAVKIADGVELQMRGQIDLVLA
                     QNDAASFAGQKIWIVDYKTGSTKELKASDLHDNLVKGTTLQLGLYSLAMCELGAAEVS
                     ASILSLAVKNVAPQLCVVDLAPHTDVFADLAEMQRTGIFGMKGEIRPAFSYSAPYPLA
                     TLPIDNDILEDKWALTHPALVLEKEEWETW"
     gene            1090..>3273
                     /locus_tag="VFO30_03755"
     CDS             1090..>3273
                     /locus_tag="VFO30_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9418433.1"
                     /translation="MVTLRDESVRARFASELDRNFSVVASAGSGKTTAITQRVLSIAR
                     LAKAEEILPRLIVVTFTNRAADEMQQRTRQALLQENFRPEVQTAFNRAFFGTIHSFCM
                     KLLTDFGHYLGLPAPLELASEDDDNLWQEFAQNQTRIGRSLGEKDRAILLRFVQARDL
                     MELARRAGSAVLQMPQLSPCPTFDFADVHAQPDRGNDNISKSQAELREWEKRYAGDWE
                     YLRWPVCFTAANARFTQLWQGKLAPLRKWVCDAATCVAAEVQRDYLDFRLDRGLVTYG
                     DQIALAKDLLQHPVAARRIREENLRVILDEAQDTEPAQFSVLLEATRPPEATGIWIED
                     RSTAHPPSQSFGAASRAVAAERDAPPRPGHFCMVGDFQQSIYWERADLNYYRAVHEAL
                     IADPHGASLEFGVTFRLDQKQLDFVNETFREILNEQHGQVPFVELQPRPNILPGKVIR
                     VPLAAKELLPEGKKLKDYQKARIEAEYLARWIKDAGLKELSADSWREVAILCPRKAWL
                     QTIAAALRRVGLPVAIQSERDVKGDSPAYAWLTALLTIMTDPFNAYEIVGVLREIFGV
                     SDHDLAVFSEAQKWRFRIDEVLSAAGKISSHLRALAEIRQHVEGLALFDAVALIVDQT
                     QLRERLLILPVTEFGDLARELDVLLAQAAEAEARGMILAEFAEHLRDDFTTPRAVRFA
                     ADDNALQLITSHKAKGSEWQAVIVPYLARDLRPHSRRYPHFVKSPV"
BASE COUNT          692 a          922 c          982 g          677 t
ORIGIN      
        1 cgaaccgctc acgcaaacga ccctgaaaaa tttgcaacgc gcgaccacgc ttttgccgaa
       61 gactgcaccg tcagcggcgg atgtgcagca aacgctgatc gcatttgacg cccggcgcga
      121 ttcttccaag cctcccggcg aatacgattt cgccttgcga cctaacgaat cctttcgccc
      181 ggtgccgatg ctaagtgtga gcgatctcga acgaatggtt tcgtcgcccg caattatctg
      241 gatgaaacat tatcttggcg tggaagcgcc ggatgacgac gcgaacccgt ggtcagcggc
      301 gaccggcaaa tgggtgcatg attggctggc gagcattgcc gagacaccgg ctggaaaaat
      361 tttcgcgccg tttccatcat caacaaagat cgacaagtgt attcggcttg ccgcggacga
      421 acgccgtgcg gcgctgcaac gtctgtgcga gttgttgggc aaggtcgtgc ctgattggtg
      481 gaactcgggc tggttgaacg cgcgttatct ggcgcggcat ctcggcagca aaatcgcaac
      541 cgccaccggc tgggattgga tggcggctga gtttcccgct ggtcgcggag acgcggtgaa
      601 aatagccgac ggcgttgagc tgcagatgcg cggccaaatc gatttggtgc tcgcgcaaaa
      661 tgatgccgcc agtttcgccg ggcaaaaaat ctggatcgtc gattacaaaa ccggttcgac
      721 caaggagctg aaagcttccg atctgcacga caatctcgtg aaaggcacaa cattgcagct
      781 tggcctctac tcgttagcca tgtgcgagct cggcgcggct gaagtaagtg cgagcattct
      841 ctcgctcgcg gtaaaaaacg tcgcgccgca attgtgcgtt gtcgatcttg cgccgcatac
      901 cgacgtcttc gccgatctcg cggaaatgca acgcaccgga attttcggaa tgaaggggga
      961 aattcggcca gcgtttagtt acagcgcgcc gtatccgctt gcgactttgc ccatcgacaa
     1021 tgacatcctg gaagataaat gggcgctgac gcatcccgcg ctcgtactcg aaaaagagga
     1081 gtgggaaaca tggtgacgct gcgcgacgaa tccgtccgtg cgcgttttgc gagcgagctg
     1141 gaccgcaatt tttctgtggt cgcatccgcc ggttcgggca agacgacggc gatcacgcaa
     1201 cgcgttttgt cgatcgcgcg cttggccaaa gccgaggaaa ttctgccgcg tctaatcgta
     1261 gtcaccttca ccaatcgcgc cgccgacgaa atgcagcagc gtacgcgaca agctttgctt
     1321 caggaaaatt ttcggccgga agtgcagacg gcattcaatc gcgcgttctt cggcacgata
     1381 cattcgttct gcatgaaact cctgactgat ttcggtcact accttggctt accggcaccg
     1441 ctcgaattgg cgagcgaaga cgacgacaat ctctggcagg aattcgcgca gaaccagact
     1501 cgcattgggc gttcgttagg cgaaaaagat cgcgccattc ttttgcgttt cgtgcaggcg
     1561 cgcgatttga tggagctggc acggcgggcg ggctccgccg tattgcaaat gccgcaactg
     1621 tcgccctgtc cgacatttga tttcgcggat gtccacgcgc aaccagacag gggcaacgac
     1681 aacatcagca agtcacaggc ggaattgcgc gaatgggaaa agcgttacgc cggcgattgg
     1741 gaatatctgc gctggccggt ttgttttacg gcggcaaacg cacggttcac acaactttgg
     1801 caggggaaac ttgcgccgct gcgcaaatgg gtgtgcgacg cagcgacgtg cgtggcggca
     1861 gaagtgcaac gtgattatct ggatttccga ctcgaccgcg gactcgtcac gtacggcgat
     1921 caaatcgcgc tcgcgaagga cttgctgcaa catccggtgg cggcacggcg gattcgcgaa
     1981 gaaaatttgc gcgttattct cgatgaggca caggataccg agccggcgca attttctgtg
     2041 ctgctggagg cgacgcgccc gccggaagcg acggggattt ggatagaaga tcggtcaacg
     2101 gcccacccgc cttcgcaaag cttcggcgcg gcaagcaggg ccgtggctgc agaacgagac
     2161 gcgccgccgc ggcctggcca tttctgcatg gtcggcgatt ttcagcaatc gatttactgg
     2221 gaacgcgccg acctgaatta ttatcgcgcg gttcacgaag cgctcatcgc agatccgcat
     2281 ggcgccagtc tcgaatttgg ggtcacgttt cggctcgacc agaagcaact cgatttcgtt
     2341 aacgaaactt ttcgcgaaat tttgaatgag cagcacggac aggtgccctt cgtcgagttg
     2401 cagccgcggc cgaacattct tcccggaaaa gtcattcgtg tgccgctcgc cgcaaaggag
     2461 ctgctgcccg aaggaaagaa gctgaaagat taccagaagg cgcgcatcga agcggaatat
     2521 cttgcgcgct ggatcaaaga cgccgggcta aaagaacttt cggccgattc gtggcgcgaa
     2581 gtcgcgattc tttgtccgcg caaagcctgg ctgcaaacaa tcgcggcggc gctgcgacgc
     2641 gttggtttgc cggtggcgat ccaatccgag cgggatgtta agggcgacag cccggcctac
     2701 gcgtggctga ccgcgctgct cacgatcatg accgatccgt tcaacgctta cgagatcgtc
     2761 ggcgtgctcc gggaaatttt cggtgtctcg gatcacgacc tcgccgtttt ttccgaagcg
     2821 caaaaatgga gatttcggat cgatgaagtt ctatcggctg ccggtaaaat ttcctcgcac
     2881 ctgcgcgcgc tggcggagat ccgccagcat gtggaaggtc tggccttatt cgacgcggtc
     2941 gcgttgattg tcgatcaaac gcaactccgc gaacgtttgc tcatcttgcc cgtgacggag
     3001 ttcggcgatc tcgcgcgcga gctcgatgtc ttgctcgcgc aagcggccga agcggaggcg
     3061 cgtggaatga ttctggcgga attcgctgaa catttacgcg acgatttcac gacgccgcgc
     3121 gctgtccgtt ttgccgctga cgacaacgcg ctccagctca tcacttcgca taaggcgaaa
     3181 ggctcggagt ggcaggcggt aatcgtcccg tacctcgcgc gcgacttgcg gccgcactcg
     3241 cggcgttatc cacatttcgt aaaatcgccc gtg
//
LOCUS       DASTDG010000119         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1073673, whole genome shotgun sequence.
ACCESSION   DASTDG010000119 DASTDG010000000
VERSION     DASTDG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073673"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFO30_03760"
     CDS             complement(<1..422)
                     /locus_tag="VFO30_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9418434.1"
                     /translation="MKGCKRMRSQDNVFYERARACLIESAEVKRLVVENCLDSIVSAA
                     ELIADAFRSGGKVLLCGNGGSAADCQHVAAEFVSILRRDFERPGLPAIALTTDTSFLT
                     AYANDFGYEGLFARQVEALGKAGDLLVGISTSGNSVNV"
     gene            complement(409..969)
                     /locus_tag="VFO30_03765"
     CDS             complement(409..969)
                     /locus_tag="VFO30_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9418435.1"
                     /translation="MMGRRFVVLDRDGTVIVKRHYLSDPDQVELLSGAATGLRRLREL
                     DLGLVLVTNQSAIGRGFFDQSRLELVHRRLRELLEAERVRLDGIYFCPHKPDDECVCR
                     KPQPGMVETAAEELDFDPRLCFVIGDNDCDVALGRRVGATTILVRTGYGAQVVAEGKA
                     HPDYVVDNLAEAAQLIERLISVDERV"
     gene            1362..2435
                     /locus_tag="VFO30_03770"
     CDS             1362..2435
                     /locus_tag="VFO30_03770"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418436.1"
                     /translation="MIISRTPFRISFFGGGTDYPTWYREHGGAVLATTIDKYCYLTCR
                     YLPPFFEHRIRLIYSKIENVQQPAELAHPSARETLRFMKIRRGVEIHHDADLPARSGM
                     GSSSAFTVGLLHALHAMKAKMPGREQLAREAIHVEQNMIRESVGSQDQVLAAYGGFNH
                     ITFSTDGHFHVRPMILPQARMRELGAHLMLFYTGIKRTASDIAATFITTINDKRRHLL
                     SMRALVDEAVKLLNSNRPIAEFGKLMDETWQLKCELSDKISNPQVEGLYNEAISAGAL
                     GGKLTGAGGGGFLLLFVPRAKQPAVRAKLRRLIHVPFRFEFFGSQIIFYDVEQDYAAV
                     EKERASRPIESFQELEEIEALSR"
     gene            2506..3213
                     /locus_tag="VFO30_03775"
     CDS             2506..3213
                     /locus_tag="VFO30_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9418437.1"
                     /translation="MISNASALDAIVLAGGMGTRLREVVADRPKVLADVAGRPFLTRL
                     LDQLIGTQVASVVLSTGYMAEMIEATIGTQYRGLPVRYSRENEPLGTGGALRLALDKT
                     QSDPLLALNGDSFCDANIGELCAFHRKKKARATLLLTRVEDASRFGSVESEANGAVTR
                     FEEKGRARAPGWINAGIYCLAREVISGIPAGRAVSMEHEIFPRLIGAGLCGFRVRGAF
                     IDIGTPHSYAEAERFFG"
BASE COUNT          823 a         1146 c         1084 g          808 t
ORIGIN      
        1 atcacgttca ctgagttccc gctggtactg atcccaacga gcaggtctcc ggccttaccc
       61 aacgcctcca cctgtcgcgc gaaaaggccc tcgtaaccga agtcgttcgc ataagcagtg
      121 aggaaggacg tgtcggtcgt cagagcgatc gccggcagcc ctggacgctc aaagtctctt
      181 ctgagaatgc tgacgaactc ggcggccaca tgctgacagt ccgccgcgct cccgccgttg
      241 ccgcatagta agaccttccc acccgaacgg aaggcgtccg cgatcagttc cgctgctgac
      301 acaatcgagt cgaggcagtt ctctacgacg aggcgtttaa cttccgcgct ctcgatgagg
      361 caggcccgag ctctctcgta aaacacgtta tcctgtgagc gcatccgctt acaccctttc
      421 atccacgctg atgaggcgct cgatcagctg cgccgcctcg gctaaattgt caacaacgta
      481 atcagggtgc gccttgcctt cagcaaccac ttgggcgccg taccccgtgc gcaccaggat
      541 tgtcgtggcg ccgacgcgcc gtcccagcgc tacgtcgcag tcattgtctc cgatgacgaa
      601 gcagaggcgc gggtcgaaat ccagctcctc agccgcggtc tcaaccattc cgggttgcgg
      661 cttgcgacac acgcactcat cgtccggctt gtgcgggcag aaatagatgc cgtccaagcg
      721 gacgcgctcg gcctccagca gctcacgcaa acgcctgtgc accaattcga gacgcgattg
      781 gtcgaagaag ccgcgcccga tggcagactg gttcgtcacg agcaccaggc ccaggtccag
      841 ctcgcgcaac cgccttaagc ctgtcgcggc accgctgagc agctccacct gatctggatc
      901 tgaaagataa tggcgcttga cgatcaccgt cccgtcacgg tcaagcacca caaagcgtcg
      961 tcccatcatg ctgcgctaac gcggcgaaga agtcctccgc cggcgtctga tttggtcatt
     1021 cgatgcagga accgcaccac ggctgtcaac tctcccgcaa gctgtggctg acttctcgac
     1081 atcgactgct aggcattctt gcgacatgag aatcgaaaat cggcctccgg cggatgtatg
     1141 cccagctgaa aattctgtag ctcatacggc gagcatgttt ttgagaaaaa gtgcgacagc
     1201 taagatcgac aactgcgaat gcgcggacga aacgctcgca atcgctgctc tgtgcatcag
     1261 ttccgaccac aaaacgtctt tataacacga acggtgagag aacacagtgg cggcgaaatt
     1321 gattgcctga agcaaggcaa ctgtgctagt tctttctatg catgatcatc tcccggacgc
     1381 cgttccgcat ctcgtttttc ggcggtggca cagattatcc gacatggtac cgcgagcacg
     1441 gcggcgcggt gctggcgact actattgaca aatactgcta cctgacgtgc cgttatcttc
     1501 cgccgttttt cgagcatcgg atcaggctga tttattcgaa aatcgaaaac gtccagcagc
     1561 ctgcggagtt agcgcatccc tccgcccgcg agacacttcg atttatgaaa atccgccgcg
     1621 gcgtcgagat tcatcacgac gcggatttgc cggcacgtag cggcatggga tcgagttcag
     1681 cgtttacggt cggtttgttg cacgcgttac acgcgatgaa ggccaagatg ccaggacgcg
     1741 aacagctggc acgtgaagcc attcacgttg agcagaatat gatccgcgag tcggtcggct
     1801 cgcaggacca ggtcctcgcg gcctacggtg ggttcaacca cataacgttt tcgacggacg
     1861 gccattttca tgtgcgcccg atgattctgc cgcaggcacg gatgcgtgag ttaggcgcgc
     1921 acttgatgct tttttatact gggatcaaac gcaccgcctc cgatatcgcg gccacattca
     1981 taacgacgat caacgacaaa agacggcacc tcctctcgat gcgtgcgctt gtcgatgaag
     2041 cggtgaagct actgaatagc aatcgaccga tcgctgagtt cggcaagctt atggatgaaa
     2101 cctggcagct caaatgcgag ctgagcgaca agatttccaa ccctcaagtg gaagggcttt
     2161 acaacgaagc cattagtgcg ggcgcgcttg gtgggaagct caccggagct ggcgggggtg
     2221 gttttctgct tctctttgtg ccgcgtgcaa aacagcccgc cgtgcgcgcc aagctccgcc
     2281 ggctcattca tgttccgttc cgattcgaat tttttggtag tcagattatt ttttacgacg
     2341 tcgagcagga ctacgcagcc gtggaaaagg aacgcgccag ccgaccgatc gaatcgtttc
     2401 aagagctaga agagatcgaa gcgctaagtc gatgacagag ttcgagctgc ggaaaacaca
     2461 accgatgctc gtgcgattcg ccatctaaaa gcgtgtgacg atgcggtgat ctccaacgcg
     2521 agcgcgctcg atgcgatcgt cctcgctggc ggcatgggca cgcgcctacg ggaagtggtg
     2581 gcagacaggc cgaaggtgct cgccgatgta gcgggcaggc catttctcac ccgactactt
     2641 gaccagctta taggcaccca ggtcgcgtcc gtcgttctct caaccgggta catggcggag
     2701 atgatcgagg cgacaatcgg cacgcaatat cgggggttgc cagttcgcta ctcccgcgaa
     2761 aacgagccgc tcggcaccgg aggcgcgctg cggctggcgc tggacaaaac gcagtccgat
     2821 ccgctgctcg cgctcaacgg cgattcattt tgcgatgcga acatcggcga gctatgtgct
     2881 tttcatcgca aaaaaaaggc gcgcgccacg ttgctgctca cgcgtgtcga agacgcgagc
     2941 cgtttcggga gcgtcgaatc cgaagcgaat ggcgcagtca cccgtttcga agaaaaaggc
     3001 cgagccaggg cgccgggctg gatcaatgcc ggcatctatt gcctggcgcg cgaagtcatt
     3061 tcgggcattc ctgctggccg cgcggtgtcc atggagcacg agatttttcc gagactcatc
     3121 ggcgcaggct tatgcggatt tcgcgttcgc ggcgcgttca ttgatattgg caccccgcac
     3181 tcctacgcgg aagccgagcg gttttttgga tgatagcggc atggccgctg tcgcggccaa
     3241 gcgcgttgac actaagtgag tttcctttgt aggtgccatg tcggcaaagc tatacaaaat
     3301 tggcgggtgg gtcaggcgac tacttatgcc gcgcacagct tctttgcgcg ccgaccggat
     3361 aacgaagctt cgttgagttc gatacatcgg gtagacttcg atgaagcgcc ggcagaatgc
     3421 acctacacgt atggtgcaac gaggctcatt ctggcgttcc tccatctgct gccaaccgaa
     3481 gccacttatg agcttgaact tcatcttgcg tcatacctaa ccctcgctcg taacaaatac
     3541 cgaggttgtt tcgagcaatg acatgccctt attctgcagc cttctcgtac acctcactct
     3601 cgaccgaaaa gatgtcgcct ggtaagtgaa tgcctcatac gcttgctgcg gacttgcgtc
     3661 cgcagaatcc gcgagccgga agaggactga ttcagtgatt tgccactaac cgccgactgc
     3721 cgcacgcaag cacgcacgca aagatcgaga gcttcattca atgtagccga ggcctttgag
     3781 tcgttcttcg acgatagcgg cttcttcgtc ggagtactgg gcccctcgtt ccgcatctga
     3841 gcccgcgctg ccgccgacct g
//
LOCUS       DASTDG010000120        15451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_67082, whole genome shotgun sequence.
ACCESSION   DASTDG010000120 DASTDG010000000
VERSION     DASTDG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15451
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67082"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VFO30_03780"
     CDS             complement(<1..1101)
                     /locus_tag="VFO30_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9418438.1"
                     /translation="MHNLNIESHLKEKRVFEPPHEFAKQARISSMKQYRRMYRESLNR
                     PEKFWAREASELVWHKRWKKLLEWKPPFAKWFVGGQLNLSENCLDRHLATARRNKAAI
                     LWEGEPGDKRTLTYQQLHHEVCRFANVLKRNQICKGDRVIIYLPTIPEAAIAMLACAR
                     IGAVHSVVFGGFSADAIRDRIGDSGAIALITADGGYRRGGIVPLKKNVDDALRQRSTI
                     RRVIVFQRAHNEIHMAEGRDVWWHRELEYVDANCPAVPLDSEHPLYILYTSGSTGKPK
                     GILHTTGGYLVGIYTTTKYVFDIRDTDIFWCTADVGWVTGHSYVVYGPLANGATTLMY
                     EGAPNWPEPDRFWKIIEEYRVNILYSAPTAIRA"
     gene            1213..1422
                     /locus_tag="VFO30_03785"
     CDS             1213..1422
                     /locus_tag="VFO30_03785"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HET9418439.1"
                     /translation="MANHIYKKIELVGSSPEGFEQAVQNALARADKTVRNMRWFEVTE
                     TRGQIEDGKVNHWQVTLKVGFTLEA"
     gene            complement(1493..1774)
                     /locus_tag="VFO30_03790"
     CDS             complement(1493..1774)
                     /locus_tag="VFO30_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418440.1"
                     /translation="MDESFAGTSEDFAQRGLRYTKEQFESVLTETENYVRARPAQALL
                     YAVVAGYLLNRLPVGRIATAMIRLLYVAMKPAVLVYGASKLYEAAQREE"
     gene            complement(1822..2733)
                     /locus_tag="VFO30_03795"
     CDS             complement(1822..2733)
                     /locus_tag="VFO30_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9418441.1"
                     /translation="MKSEWIDATLLRAFEAQGTDAHRLCTIPDGWVERFGSDALISFK
                     NVATRDRLLCELNCWGTAVGFALGRIFGRLLPKRNEEREKPRLMFGDRQGNLQTTATE
                     RHLKFGIDFGAGYSVGLFVDQRENRSYIRRVKPKRLLNCFAYTCSFSVAATSVGASTV
                     NVDLSKKSLTRGRENFALNRLATSGHHFIVDDVMSVLPRLARRGEKFNAIILDPPTFS
                     RNSRGDSFHVEQDFERLLNLALEVAVQDARILLSTNCSALGHHALEGMARHSLKLCRR
                     AGKFARPARLPDFPPGAGAKSVWLLLC"
     gene            2866..3768
                     /gene="thrB"
                     /locus_tag="VFO30_03800"
     CDS             2866..3768
                     /gene="thrB"
                     /locus_tag="VFO30_03800"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET9418442.1"
                     /translation="MQQVTVRVPASTSNLGPGFDCLGVALRIYKDVTVTPGSTLPLPT
                     IASDAAKLFWQRARCRSLPFSVSMAGDIPRARGLGSSAAVRTGILFGLNQLCGSPLDQ
                     LSIFRLCAQIEGHPDNAAPAVFGGFNVIRASVVQHFPVSPRLKFVLLIPEVELKTSTA
                     RKILPARVTRGAAVENCANACAITAAFVSRQYEQLRGAFVDKLHQPFRAKLLTFLPRV
                     IAAAEKAGALGAFLSGSGSTICAVTLADAERIAAAMQRKISPPQNGFAVANNYAARVV
                     ITEADNRGARVRSIRNPPSASRTL"
     gene            3768..4958
                     /gene="lpxK"
                     /locus_tag="VFO30_03805"
     CDS             3768..4958
                     /gene="lpxK"
                     /locus_tag="VFO30_03805"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9418443.1"
                     /translation="MSRWRENLEQFAIDVILERRHGTRAGILRGFLYVLSLIYERLVQ
                     LRLYFYRKRIFRERALGCLVISIGNLTVGGTGKTPIVEKFARALQSGGRRVAILSRGY
                     KSVPRKRTLPDRLLKRDFHPPRIVSDGKSLLLDSLTAGDEPYMLAHNLQDVIVLVDKD
                     RVKSGRFAIDKWKVDTLVLDDGLQYLHLKHRLDMVLVDRQAPFGNEFLLPRGTLREPP
                     RNLRRASYIFITKSTGESNQPLVEKIRRCNRTAEIIECAHKPLYLQNVFTGERQPLEH
                     LRGTFIGSLCAIAAPESFEDGLKKLGAEVNLAKRYIDHHYYTEAELLSFIKRCIRRDL
                     AMIVTTEKDAVRMPRLPESELKVPIYFLRVEIEILSGHESWDHCVARICKPQPMLPPE
                     RFFA"
     gene            5012..5929
                     /gene="ligD"
                     /locus_tag="VFO30_03810"
     CDS             5012..5929
                     /gene="ligD"
                     /locus_tag="VFO30_03810"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9418444.1"
                     /translation="MSEKAQLVVEGKRLAVSNLNKVLYPKVGFTKGQVIDYYIRIAPV
                     LLPHLRDRPLTMKRYPNGVDAQFFYEKNCPSHRPSWVRTAKVWSEGNQRIMDYCLAND
                     LPTLVWAANLADLELHTSLSRKHDVARPTMMVFDLDPGPPADMVQCCQVGLWLRDLLG
                     EMKLKSWAKTSGSKGLQVYVPLNTSVTYDDTKGRARSLAEHLEREHFDLVVSKMLKSL
                     RSGKVLVDWSQNDEHKTTVCVYSLRAKDEPTVSTPVTWDEVESCLKKKKAALLKFRSD
                     QVLARVEKIGDIFAPIEKLKQKLPKKREL"
     gene            5938..6192
                     /locus_tag="VFO30_03815"
     CDS             5938..6192
                     /locus_tag="VFO30_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418445.1"
                     /translation="MNNLIWFDAIGWIGAVALLLAYAMVSFNKVAGDSPAYQLLNIAG
                     SILLAANTIAYRAYPSTFVNLIWIGIAGFTLATRKRRHCN"
     gene            6341..7390
                     /locus_tag="VFO30_03820"
     CDS             6341..7390
                     /locus_tag="VFO30_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET9418446.1"
                     /translation="MTSSDAPSTRRRVRVWGQLGIWTAALCVVAFIIWQAVAVHGAPD
                     PLKPHTSATVAILDIAVLVFREGLECILVLAAITASMTGAKRVLRRPVAVGAGGAFLA
                     TLITWWIAVQIMGQLSENVRALDLQAATGLLAVVVLLVIMNWFFHKIYWGGWIRAHNR
                     RRKALLANGAGAEISRAHLRWGLILLGFTSLYREGFEVVLFLQSYRLRLGSGVVLQGA
                     LVGVALAAVVAILTFFVQQRLPYRKMLITTGVLLGVVLLVMVGEQAQEMQLAHWISTT
                     PVPWLIGLVPQWMNLWFSIFPTYETLLAQLFAAALVIGSYYAARHFGGGPPAEPEPIA
                     KVRVEATAAIAEQVV"
     gene            7504..8187
                     /locus_tag="VFO30_03825"
     CDS             7504..8187
                     /locus_tag="VFO30_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418447.1"
                     /translation="MRHFTFLYEAPTSPPGSFELENWLTWARTSEPAHADELAFRHEL
                     EIGVTNHFQAGIYFADWAYTHANGGSGFNYSDAALELIYNFSNPVIDPLGLSIYQEFK
                     AGDRLLQWESKIIAQKNLGPLILAYNMTLEAVWEGDDLRKTEGELQQAVGASYELSPR
                     LAAGIEFLHEFVFPHWRDAQAISNIFVGPNVSYRRSNWFVTVTALAQATRTEEEPDFQ
                     LRTIFGIGL"
     gene            8188..8478
                     /locus_tag="VFO30_03830"
     CDS             8188..8478
                     /locus_tag="VFO30_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9418448.1"
                     /translation="MRFSILLPGIALLTSCQWTRYYTPPPIAQRTVDLALVQKGRRLF
                     AQRCIECHTLPPVWRYQAEDWPQIVNSMAHRAALTSGEREAIIAYILAARAE"
     gene            8589..11831
                     /locus_tag="VFO30_03835"
     CDS             8589..11831
                     /locus_tag="VFO30_03835"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418449.1"
                     /translation="MIMPILRSRSLLQLISAVFVCSISFAANPTPPRGGGPGVQPASA
                     TSPTTSPSPPRPGTIEERLFNGMQWRQIGPFRGGRALAIEGVVGEPDTWYFGAVAGGV
                     WKTVDGGQTWTPLFDKQDISSIGAIAVAQSDHNVVYAGTGEGAIRGNTTYGLGVYKSI
                     DAGKTWNNVGLRDTQQIGALIVDPRNADVVIVAAVGHAFGPNSERGIFRTSDGGKTWT
                     KVLSKDENTGGIDVVFDPHNPNIVLASLWQARRQPWFFSSGGPGSGLYRSEDNGITWK
                     QLQGNGLPEGILGKIGISVSGADPNRIYALIEAKEGGIYRSDDAGQHWTRVNDDGRFR
                     QRAWYFTKIYADPKSADTVYVLNTGAFRSVDGGKTFELLPARHGDHHGLWIDPQNPNR
                     IGNANDGGVSISTDGGKTWTTQNNQPTAQFYHVAVDNAFPYHIYGAQQDNSNVGIASR
                     SDSGVIGREDWFQAGGGECGFVVPDPRDWHIIYSNNEGYAVRYDKTRENSQDISPVPL
                     DNSGHGAVNLAHRFQWVSPLMLSPHDPDTLYTAGEVVWKSTDHGNSWTQISGDLTRND
                     KSKQQPSGGPLTNDITSVEYYDTIFALTESPVKRGTLWAGTDDGLVHVTTDDGQHWTK
                     VTPKMPEWSTVDLIEASPHDANTAYVAVDRHKLDDFKPYVFRTTDLGKTWSSIVNGIP
                     DRAYVHAVREDPKKRGLLYAGTELGVFVSFDDGAHWQPLQLNLPPSPIHDLVVKDDDL
                     VVATHGRSFWVLDDLTPLRQVTAQSAGADTVLYQPQTALRLHYPEEIDKKQPVGDNPP
                     PGAIINYYLKSAPKDEVTLDILDASGKLVRHLSNKEKKDAAQPPEWPDRVERVKTIPA
                     HEGMNRFAWDLRYDDPIQIPGAFYSGSGPKGPLALPGDYQVKLTVAGKNQTAPLHLVI
                     DPRTKGAEAAVQKQFEFAAKVNDRISQLHQAVNEIREIRSQIDSLHTRFGEEERTKPA
                     LAAADGLNRKMSEVEQQLIQVNMKGSEANLAFPNMLNERFDSFSHIIEVGDTAPTKSQ
                     LDVFQLLSGQLDQQLNRWGQIKNDDLPKVSAAIKQLDLPALIVTPPAKSL"
     gene            complement(11898..12302)
                     /locus_tag="VFO30_03840"
     CDS             complement(11898..12302)
                     /locus_tag="VFO30_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418450.1"
                     /translation="MAALQFLGKYRETGLLLIRVSVGLIFIILLGPVLWNPGSWDRIG
                     SAMRYLDFHSHYKFWGIVGALLGCIGGVLMVLGLLFRIGVLFALIVTLVHLVALWSAR
                     GGLYARLPALEMSILLLSLLLVGPGKYSVDKN"
     gene            12449..13150
                     /locus_tag="VFO30_03845"
     CDS             12449..13150
                     /locus_tag="VFO30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9418451.1"
                     /translation="MVQIDKAVLLAAGRGTRMRELTAKVPKPMIEVRGKPVLEHIVDG
                     LRNAGVQRFLVVVGYRADAVQNFFGDSVEYATQEVQDGTGRVVDLARDFVSASPFVLG
                     YGDILVDPANYRSIVDLPDEVEAIVSVTRAEDVSKGGAVAVNERMEVVDIREKSPPGE
                     APSPWYNAGLYAFRPTIFEFTAKLKPSPRGEYELTDAIRELAQSGKRVKALELVGEWA
                     DVRDPETLARLNRTL"
     gene            13243..13530
                     /locus_tag="VFO30_03850"
     CDS             13243..13530
                     /locus_tag="VFO30_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET9418452.1"
                     /translation="MPAYVIVEIDIVDPLGYDEYKKLAGATVEKYGGKYIVRGGKSEV
                     LEGDWHPKRIVVLQFDSIERAKQWLNSEEYRGPRKMRHATAKTNMLVVEGF"
     gene            13593..14189
                     /locus_tag="VFO30_03855"
     CDS             13593..14189
                     /locus_tag="VFO30_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418453.1"
                     /translation="MLVGGNAVNLYAYRRTTFDVHLLVRENDAQRWLTFFEQRGYRVF
                     HRTDNFIRMHFSTDPTKALPVDLMLADDETFRKIRAEGRRYDVGAGLELAIPSPLHLI
                     AMKLHALKSQVPVVSRPGSQRHAPNSSPTSADKQTHNHPGLPDLPQFDSLPATAGLNN
                     VEAFRLSIQHALALLPTILATADSFTAVASHPERFSLR"
     gene            complement(14252..>15451)
                     /locus_tag="VFO30_03860"
     CDS             complement(14252..>15451)
                     /locus_tag="VFO30_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418454.1"
                     /translation="HGKPEHGEGAFYVWTKKQIDKALGDAAELFNFHYGVQPHGNAPE
                     GSDPQDEFRGKNILIERHTIAETAQRFGRTEEEVQQSLARSREKLTSIRNKRPRPHRD
                     DKIIAAWNGLMISAYARAAQVLDDTRYLGPAARAAQVIRQRLYDASAKILYRTYREGR
                     SDVAGFADDYAFVIQGLVDLYEASFDSEWLKFAIELQETLDRLFFDAEHGGYFSTSEK
                     DSGVPLRLKDDNDSAEPAASSVAALNLLRLAQFRNDQILADRGRKTIRAFAATLSHFA
                     SAMPQMLVALDFSLAKPRQIVIAGAPPRVRPVAEKVDPPQDGFAVANNARTQLLLKEV
                     HQHFLPNKILALADGDEGQNYLGENNEAIRAMSLIKGKPAAYVCENFTCKAPVTEPEQ
                     LRELLTL"
BASE COUNT         3258 a         4505 c         4482 g         3206 t
ORIGIN      
        1 cgcgcgaatg gccgttggag cgctgtagag aatgttaacc cgatattcct cgataatctt
       61 ccagaacctg tctggttcgg gccagttcgg cgcaccttca tacatcaacg tggtagcgcc
      121 gttcgccagg ggaccataaa cgacataact atggcccgtc acccagccga cgtcagccgt
      181 gcaccagaag atgtctgtgt cgcggatatc gaacacgtac ttggtcgtcg tataaatccc
      241 gacaagatac ccgccggtcg tgtgcagaat gcccttcggc tttccggtcg aaccgctggt
      301 gtaaagaata tataggggat gctcactatc gagcgggacc gcagggcagt tggcatccac
      361 atattccagc tcccggtgcc accagacgtc gcggccctcg gccatgtgga tctcattgtg
      421 agcgcgctga aacacgatga cgcggcgaat tgtcgatctt tgacgcaacg cgtcatcgac
      481 attcttcttc aacggcacga tcccaccgcg acgataaccg ccgtctgctg tgatgagagc
      541 gatcgcgcca ctgtcgccga tgcggtcgcg aatggcgtcg gcgctgaatc cgccgaagac
      601 gacagaatgc accgcgccaa tgcgtgcgca agccagcatc gctatcgctg cctctggaat
      661 agttggcaaa tagatgatca cgcgatcgcc tttgcagatt tggttgcgtt tcagcacgtt
      721 tgcgaatcgg caaacttcgt gatgcagctg ctgataagta agagtgcgtt tgtcacccgg
      781 ctccccctcc cacaggatcg ccgctttgtt gcgacgcgca gttgcaagat gtcgatccag
      841 gcagttttcg gatagattga gttggccacc gacgaaccat tttgcgaagg gcggtttcca
      901 ctcgagcagt tttttccatc gcttgtgcca aaccagctcg ctggcctcgc gcgcccagaa
      961 tttctccggc cgattaagcg actcgcggta catccgccgg tattgcttca tcgagctgat
     1021 gcgcgcctgc ttggcgaatt cgtgcggcgg ttcaaagacg cgtttctcct ttagatgcga
     1081 ctcgatgttg agattatgca tgcgcagctt ctaattaggc tgaagcgaat ttcagggcaa
     1141 gcgctgcctg gcgcgccgta gcggagcgaa gcgcctgctt gccgacgctc tgttcgtcgc
     1201 cgaaatcaga gaatggctaa tcacatttac aaaaagatcg aactcgtcgg atcatctccc
     1261 gagggtttcg agcaggcggt gcagaacgcg ctcgcgcgag ccgacaaaac tgttcgcaat
     1321 atgcgctggt tcgaagtgac cgagacgcgt ggacagattg aggacggcaa agtaaatcac
     1381 tggcaggtca cgctcaaagt cggctttacc ctggaagcgt gaggtgtagc cgcggctatc
     1441 ggcgactgtc cccgccacga tctactgcgc gtgaggacac gcgcctctag agttattctt
     1501 ctcgttgcgc ggcttcgtaa agcttgcttg cgccgtaaac gagaacagcc ggcttcatcg
     1561 ccacatacag cagccgtatc atggccgtgg cgattcgccc cacaggcagc cggttaagaa
     1621 gatatccggc cacaactgca tagagaagcg cctgtgccgg tctcgctcgc acataatttt
     1681 cggtctcggt gagaactgac tcgaattgct cttttgtgta gcgcagaccg cgctgggcaa
     1741 aatcctctga tgtgccggca aatgattcat ccatacgttt aaaattcgca tcgcgatgcg
     1801 cgtctgcgcg cacgcatccg ttcagcacaa taataaccag accgactttg cgcctgctcc
     1861 aggcgggaaa tccggcaatc gcgctggccg cgcgaatttg ccggcgcgac ggcaaagttt
     1921 gagggagtgg cgtgccatcc cctcaagggc gtggtggccg agcgcactgc aattggtcga
     1981 gagcaggatg cgcgcgtcct gcacggcaac ctccaacgcc agattcagca atctttcgaa
     2041 atcttgctcg acatgaaacg agtcgccgcg cgagttcctg gaaaacgtcg gcgggtcgag
     2101 aataatcgca ttaaactttt caccgcggcg cgcgaggcgc gggagaacag acatcacgtc
     2161 gtccacgatg aagtgatggc cactggtcgc caggcggttt agtgcaaagt tctcccgccc
     2221 gcgcgtgagc gacttcttgg aaaggtcgac gttcaccgtg ctcgcgccga ccgatgttgc
     2281 cgcgaccgag aaggagcacg tataggcgaa gcaattcaac aaccgcttcg gcttaactcg
     2341 gcggatgtag cttcggtttt ccctttgatc aacgaacagt ccaaccgagt aaccggcccc
     2401 gaaatcgatt ccaaatttaa ggtgccgttc tgtcgccgtg gtttgcaggt tcccctggcg
     2461 gtcaccgaac atgaggcgcg gcttctcgcg ttcctcattt cgtttcggca acaatcgccc
     2521 gaaaatccgg cccaaggcga agcccacggc tgttccccag cagttcagtt cgcaaaggag
     2581 acgatcgcgc gtggcgacat tcttaaacga gatgagcgcg tcggacccga atcgttctac
     2641 ccagccgtcg gggatcgtgc ataaccgatg tgcgtctgtc ccttgcgctt cgaaagcgcg
     2701 cagcagggtt gcgtcgatcc attcggactt cactgtccga tcggcgtgac ggttgaccgc
     2761 agaaaccggc aaggcagatc gacccatcac cgcggttgct ggtgtagtta ggcgcattag
     2821 aatcgggcgt tattcagcgc cctcgatccg ggcgaggttt tcctcatgca acaagtcacc
     2881 gtgcgcgttc ctgcgagtac ttcaaatctt ggcccaggct tcgattgtct cggcgttgcc
     2941 cttcgcattt acaaggatgt aacggtcacg cccggatcga ccttgccgct ccccaccatt
     3001 gcctctgacg cggcgaaatt attttggcag cgcgcgcggt gccgatcact cccattctcc
     3061 gtctcaatgg caggagacat tccgcgggcg cgcggcttag gcagcagcgc agcggtccgt
     3121 actggaattc tcttcggatt gaatcagctt tgcggcagcc cgctcgatca attgtcgatc
     3181 ttccgtttat gcgcgcaaat cgagggccac ccggataatg ctgcgccggc tgtattcggt
     3241 gggttcaacg tcattcgggc ctcggtcgtg caacattttc ctgtgtcgcc gcggctgaaa
     3301 ttcgtgctgt tgatcccaga agtggaattg aaaacctcga ccgcgcgaaa aattttgccc
     3361 gcgcgagtga cacgcggcgc tgccgtggaa aattgcgcaa atgcctgcgc tatcactgcg
     3421 gcgttcgtct ctcgccagta cgagcagctg cgcggcgcgt tcgtcgacaa actgcaccag
     3481 cccttccgcg cgaagttgct tacttttctg ccgcgcgtca tcgctgcggc ggaaaaggcc
     3541 ggcgcgctcg gcgcattcct cagtggcagc ggatcgacaa tctgcgcagt aacgctggcc
     3601 gatgcagaga gaattgctgc cgcaatgcaa cgcaagatca gtccgccgca gaacggattc
     3661 gccgtggcga acaactacgc agcgcgagtc gtgatcaccg aggccgataa tcgaggggcg
     3721 cgcgttcggt cgatccgcaa tccgccatcg gcgagccgca ctctgtaatg tcccgctggc
     3781 gcgaaaatct cgagcagttc gcaatcgatg tcattctcga acgccgtcac ggcacacgcg
     3841 ccggcatatt gcgcggtttt ctttacgtgc tgtcgctgat ttacgagcgg ctggttcagt
     3901 tgcgcttgta tttttatcgg aagcgaatct ttcgggaacg cgcgcttggc tgtctcgtca
     3961 tcagcatcgg caatttgacc gtcggcggta ccggcaaaac gccgatcgtg gaaaagtttg
     4021 cgcgcgcgct gcaatccggc ggccggcgag tggccatctt gagccggggc tacaaaagtg
     4081 ttccgcgcaa acgcactctg cccgaccggc tattaaaacg agacttccat ccgccacgca
     4141 tcgtctccga cgggaaatcg ctcctgctcg attcgctcac ggccggcgac gagccctaca
     4201 tgctcgctca caacttgcaa gacgtgatcg tgctggtgga caaagatcgt gtgaaaagcg
     4261 gccggttcgc gatcgacaaa tggaaagtcg acacactcgt gctcgatgac gggttgcaat
     4321 acctccacct caagcatcgc ctcgacatgg tgctggttga tcgacaagca ccattcggaa
     4381 acgaattcct cctgccgcgc ggaacgttgc gcgagccgcc gcgcaacctg cgtcgcgcca
     4441 gctacatctt catcacaaaa agcactggtg aatcgaatca gccgcttgtc gagaagattc
     4501 gtcgctgcaa ccggaccgcg gagatcatcg agtgcgcgca caaaccgctt tatctgcaga
     4561 acgtgttcac cggcgaacgc cagcccctag agcatttgcg cggcacgttc atcggctcgc
     4621 tctgcgccat cgccgcgccg gaaagcttcg aagatggcct caagaaactc ggtgcagaag
     4681 tcaatctggc caagcgctac atcgaccacc actactacac ggaggcggaa ttactcagct
     4741 tcatcaaacg ttgcatccgg cgcgatttgg cgatgatcgt cacgaccgag aaagacgctg
     4801 tgcgcatgcc acggttgcct gagagcgaat taaaagtgcc gatttacttt ctgcgtgtcg
     4861 aaatcgagat cctgagcggc catgaaagct gggaccattg cgtcgcgcga atctgcaagc
     4921 cgcagccgat gctcccgccc gagcgcttct tcgcctgatt gtctgaattg gagattggac
     4981 gttcggcgtt cggcgttcga cgtttgccga aatgtctgaa aaggcgcagc tcgttgtcga
     5041 aggaaaaaga ctcgccgtct caaacctgaa caaagtgcta tacccaaagg tggggttcac
     5101 caaagggcag gtgatcgact attacatccg gatcgcgccg gtcttgttgc cgcatctgcg
     5161 cgaccggccg ctgacgatga aacggtatcc gaatggcgtc gacgcccagt ttttctacga
     5221 aaagaactgc ccgtcgcacc ggccgagttg ggtcaggacg gcgaaggtct ggagcgaagg
     5281 aaatcagcgg atcatggatt actgcctggc gaacgatctg ccgacgttgg tttgggcggc
     5341 gaatctggcc gatctcgaat tgcacacttc gctctcgcgc aagcacgacg tggcgcgccc
     5401 gaccatgatg gtcttcgacc tcgatccggg gccgccggcc gacatggtcc aatgctgtca
     5461 ggtcggactc tggctgcgcg atttgttagg cgaaatgaag ctgaagagct gggccaaaac
     5521 gagcggctca aaaggccttc aggtttacgt gccgctcaat acgtcggtga cttacgacga
     5581 taccaagggg cgcgcgcgct cgctggccga gcacctcgag cgcgaacatt tcgatcttgt
     5641 cgtgtcgaaa atgttaaaat cgctgcgctc gggcaaggtg ctggtcgatt ggagccagaa
     5701 cgacgaacac aaaacgaccg tatgtgtcta ttcgctgcga gccaaagacg agccgaccgt
     5761 ttccacgccc gtgacgtggg acgaagtcga gagctgcctg aaaaagaaaa aagccgctct
     5821 actcaaattc cgctccgacc aggtcctcgc ccgcgtggaa aagatcggcg atatcttcgc
     5881 gccgatcgag aaactgaaac aaaagctgcc gaagaagcgg gagctgtgat gcaaaacatg
     5941 aacaatctca tctggtttga tgcaatcggt tggataggag ccgtggctct cctgctcgcc
     6001 tacgcaatgg tgtcgttcaa caaagtcgcg ggcgactcac cagcttatca actgctcaac
     6061 atagctggca gtattcttct ggcagcgaac acgatcgcgt accgagctta cccgtcgact
     6121 tttgtaaacc tgatctggat cggaatcgct ggctttacat tggcgacccg aaaacggcgt
     6181 cactgcaact gagccggtag atccgttggc gcgtttggtt tgatcgctgg cgtcagcgaa
     6241 tcgtggatcg tttcgctttt gctttatggc tggcaagcag gcgctaaaaa atgacaactg
     6301 accgacagcg tgcggtttgc ccgtgtgata cggttctgat gtgactagct ccgatgctcc
     6361 ttcgacgcgc cgacgggtac gagtctgggg tcagctcggg atttggacgg ccgcgctctg
     6421 cgtggtcgca tttatcatct ggcaagcggt agctgtgcat ggcgcgcccg atccgctcaa
     6481 gccgcacacg agtgcgacgg tggcgatcct cgacattgcg gtgctggtat ttcgcgaagg
     6541 attggaatgc atccttgtac tggcggcgat caccgccagc atgaccggcg cgaagcgcgt
     6601 gttgcgtcgc ccggtcgcag tcggtgccgg tggcgcattc ctcgcgacgc tcatcacgtg
     6661 gtggatcgcc gtgcagatca tgggtcaact cagcgaaaac gtgcgcgcgc tcgatctcca
     6721 agccgccacc gggctgctcg ccgttgttgt tttgctcgtc atcatgaatt ggttcttcca
     6781 caaaatttat tgggggggct ggatccgcgc acataaccgg cgccgcaaag cgctcctcgc
     6841 caacggcgcg ggcgcggaaa tttcgcgcgc gcatttgcgc tggggattga tcttgctcgg
     6901 attcacgtcg ctttatcgcg aaggcttcga agttgtgctg ttcctgcaaa gttaccggct
     6961 gcggctcggc agcggcgttg ttttgcaagg cgcgctcgta ggcgtggctc tcgccgcggt
     7021 cgtcgccatt ctcaccttct tcgtgcaaca gcgtttgccc taccgaaaga tgctgatcac
     7081 gacaggtgtg ctgcttggag tggtgttgct ggtgatggta ggcgaacagg cgcaagagat
     7141 gcagcttgct cactggatat caacgacgcc ggtgccgtgg ctcatcggtc ttgttccgca
     7201 gtggatgaat ctctggttct cgatttttcc cacctatgaa acgctcctcg cacaactgtt
     7261 cgccgcggcg cttgtaatcg gctcctacta cgcggcgcgg catttcggcg gcgggccgcc
     7321 cgccgaaccg gagcccattg caaaagtgcg ggtggaagca accgcagcca tcgccgagca
     7381 agtcgtgtag cggcgtttgt gtcaaacgcc gagctgaaac gcaggcactc ggcgcggcga
     7441 gcgcctctac aattgtcgtt ggtgtgcgcc tcgatctttt gcgcggcatc tgcattcgcc
     7501 ggcgtgcggc atttcacatt cctttacgaa gcgcccactt cgccgcccgg tagctttgaa
     7561 ttggaaaatt ggttgacgtg ggcccgaacg agcgaaccgg cacacgcgga tgagctcgcg
     7621 tttcgtcacg agctcgagat cggtgtaacg aaccattttc aagctggcat ctatttcgcc
     7681 gattgggctt acacgcacgc gaacggcggc tccggtttca actattccga cgccgcgctt
     7741 gagctcatct acaatttttc caatccggtg atcgacccgc ttgggctgtc gatctaccag
     7801 gaattcaaag cgggcgaccg attgctccag tgggaatcga aaattatcgc gcaaaaaaat
     7861 ctcgggccac tgatcctcgc ctacaacatg acactcgaag ccgtctggga aggcgacgat
     7921 ttgcgtaaaa ctgaaggcga actgcagcag gcggtgggcg ccagttacga gctatcccca
     7981 cggctcgccg cgggcatcga gttcctgcac gagtttgtct ttccgcattg gcgggacgcg
     8041 caagcaatta gcaacatttt tgtcggacca aatgtttctt accgtcgcag caattggttc
     8101 gtcactgtta ccgcgctcgc gcaagcgact cggacagagg aggagccgga ctttcagttg
     8161 cggacaatat tcgggatcgg tctttaaatg agattctcga ttttgctgcc gggcattgcg
     8221 ctgctcacct cttgccagtg gacgcgctat tacacgccgc cgcccattgc gcagcgaact
     8281 gtcgatcttg cgctcgtgca aaaaggacgt cgactgtttg cccagcgctg catcgagtgc
     8341 cacaccctgc cgccggtctg gcgttatcag gctgaagatt ggccgcagat cgttaattcg
     8401 atggcacacc gcgcggcgct cacatccggc gaacgtgagg cgatcattgc gtacatcctc
     8461 gccgcccgcg ccgagtaggc ccgcgcctat ggcgcgtcgt ctaacgcaat gattacgctg
     8521 acggcccaca cggccgtggc tacaggattc cagttgaacg agcggccaag ctttcccaca
     8581 ctacgcggat gattatgccg atcttgcgtt cgcgttcgct tcttcaactc atttccgcgg
     8641 ttttcgtttg ctcgatttca ttcgcggcca atccgacccc gccgcgcggc ggcgggccgg
     8701 gcgtgcagcc ggcgagtgct acttcaccga ccacgtcgcc ctcgccacct cgccccggca
     8761 caatcgagga aagactgttc aatggcatgc aatggcggca gatcggcccg ttccgtggcg
     8821 gacgcgcgct cgccattgaa ggggtcgtgg gcgaaccgga cacatggtat tttggcgcgg
     8881 tcgcgggcgg cgtttggaaa acggttgatg gcggccaaac ttggacgccg ctcttcgata
     8941 agcaggatat ctcgtcgatc ggcgcgattg cggtcgcgca atctgatcac aacgttgttt
     9001 atgcgggaac aggcgaggga gcaattcgcg gcaacaccac ctacggcctc ggtgtttaca
     9061 aatcgatcga tgccgggaag acgtggaata atgttgggct acgagacacg cagcagatcg
     9121 gcgcgttgat tgtcgaccca agaaacgctg acgtggtcat cgtcgccgca gtcggccacg
     9181 ccttcgggcc gaacagcgag cgcggaattt ttcgcacgag cgacggcggc aagacgtgga
     9241 cgaaagtatt gagtaaagac gagaacaccg gcgggatcga cgtggttttc gacccacata
     9301 atccgaacat cgtcctcgcc tctctttggc aggcgcggcg gcagccgtgg tttttctcca
     9361 gcggcgggcc gggcagcggg ttgtatcgct cggaagacaa cggcatcact tggaaacagc
     9421 tccaaggcaa cggattgccc gagggcattc tcggcaagat cggcatctcg gtttccggcg
     9481 ccgatcccaa tcgcatctat gcgctcatcg aggcaaagga aggcggaatt tatcgctcgg
     9541 atgacgcagg gcagcattgg acacgcgtga acgatgacgg ccgtttccgc cagcgcgcgt
     9601 ggtatttcac taaaatttat gccgacccaa aatcggccga cacggtttat gttttgaaca
     9661 ccggcgcgtt tcgatcggtc gatggcggca aaacttttga gcttctgccc gcgcgccacg
     9721 gggatcatca cggcctctgg atcgatccgc aaaatcccaa tcggatcggt aacgcgaacg
     9781 acgggggcgt cagcatttcc accgatggcg gcaaaacctg gacgacgcaa aataatcaac
     9841 caacggcgca gttctatcac gtggccgtgg acaacgcctt tccgtatcac atctacggtg
     9901 cgcagcagga taactccaat gtcggcattg ccagccgcag tgactcgggc gtgatcgggc
     9961 gtgaagattg gttccaggcg ggcggcggcg aatgcgggtt cgtcgtgcct gatccgcgcg
    10021 actggcacat catttactca aataacgagg gctacgccgt tcgttacgat aaaactcgcg
    10081 agaactccca ggatatcagc ccggtgccac tcgataactc cggccacggc gcggtcaatc
    10141 ttgcccatcg cttccagtgg gtttcgccgc tcatgctctc tccgcacgat cccgacacgc
    10201 tttacaccgc cggtgaagtg gtctggaaat cgaccgatca cggcaacagc tggacgcaaa
    10261 tcagcggtga cctgacccgc aacgacaaga gcaaacagca gccaagcggg gggccgctga
    10321 ccaacgacat cacctcggtt gaatattacg acacaatctt tgccttaaca gagtcgccgg
    10381 tgaagagagg gacgctttgg gcggggacgg atgatggact cgtgcacgtc acgacagacg
    10441 acggccagca ttggacgaaa gtcacgccga agatgccgga atggagcacg gtggatctga
    10501 tcgaggcatc gccccacgat gcgaacactg cgtatgtagc ggtagatcga cataaactgg
    10561 acgatttcaa gccctacgtt ttcaggacca ctgacctcgg caaaacctgg tcgtcgattg
    10621 tgaacggaat tccggacaga gcgtacgtgc acgccgtgcg cgaagatccg aagaagcgcg
    10681 ggctgcttta cgccggcacg gagcttggcg tgttcgtgtc gtttgacgat ggcgcgcatt
    10741 ggcaaccgtt gcagttgaac ctgccgcctt cgccgattca cgatctggtg gtgaaagacg
    10801 acgatttggt cgtggcgacg cacggccgct ccttctgggt gctcgacgat ttgacgccgt
    10861 tgcgacaggt caccgcgcaa tcggccggtg ccgacacggt cttatatcaa ccgcagacgg
    10921 cactgcgttt gcactatccg gaggagatcg acaagaaaca gccggtcggc gataacccgc
    10981 ctccgggcgc gatcatcaat tattatttaa agagcgcgcc gaaagacgag gtgacactcg
    11041 atattcttga tgcatccggc aaactcgtta ggcatctctc gaacaaggag aagaaggacg
    11101 ccgcccagcc gccggaatgg cccgaccgcg tcgagagggt gaagacgatt ccggcacatg
    11161 aagggatgaa ccgtttcgcg tgggacctgc gctatgatga tcccatccag attccaggcg
    11221 ccttttattc tggcagcggt ccgaagggac cgctcgcgct gcccggcgat taccaggtga
    11281 aactaacggt ggccggaaaa aaccagaccg cgccgttgca tttggtcatc gatccgcgaa
    11341 cgaaaggcgc ggaggcggcg gtgcagaagc agttcgagtt cgcggcaaag gtgaacgatc
    11401 gcatttcgca gctgcaccag gcggttaatg agatccgcga aatccggtcg caaatcgatt
    11461 cgctccatac gcgtttcggc gaggaagagc gaacaaaacc ggcgcttgcc gctgccgacg
    11521 gactcaaccg caaaatgtcc gaggtcgagc agcaactgat tcaggtcaac atgaaaggct
    11581 cggaagcgaa tctcgctttc ccgaacatgt tgaacgagcg gttcgacagt ttcagtcaca
    11641 tcatcgaagt gggcgacacc gcgccgacca aatcccagct ggacgtcttt cagctgctca
    11701 gtgggcagct cgatcagcaa ctcaaccgct gggggcagat caagaatgac gatctgccga
    11761 aagtgagcgc cgcgatcaag caactggatt tgccagcgtt aatcgtgaca ccgccggcga
    11821 agtcgctgtg agaaatgaga agtgaaaagc ggagagggga cttagagagg tctcacttct
    11881 cacccatcac ttctgactta atttttatct acgctgtatt ttcccgggcc gacaagcagc
    11941 aggctcaaca gcaagatcga catctcgagc gccggtaagc gcgcatagag accgccgcgc
    12001 gcgctccaaa gcgcgaccag gtgcacgagc gtgacaatca gtgcgaacaa aacgccgatg
    12061 cgaaacaaca agccgagcac catcagcacg ccgccgatgc atccgagcag cgcgccgacg
    12121 attccccaga atttgtagtg cgaatgaaaa tcgagatagc gcatggccga gccgattcgg
    12181 tcccagcttc ccggattcca caacaccggc ccgagaagaa tgatgaaaat caacccgacg
    12241 ctcacccgaa tcagtagcag acccgtctcg cggtacttcc cgagaaactg caacgcagcc
    12301 atgcccgcga ctctgattga gcgcgatgaa gatttcaatg caaaggtgga tcggccgctc
    12361 cgcgggcgat gctaaataat tgcggcttcg ccgcctcatt ttaacatcga gcgacggagc
    12421 ggctcgatcc accagctcaa tttcgtttat ggttcagatt gataaagccg tgctgctcgc
    12481 ggcagggcgg gggacgcgaa tgcgcgagct gacggcaaag gtgccgaaac cgatgatcga
    12541 agtacgcggg aagccggtgt tggagcatat cgtggacgga ttgcgtaatg caggcgtgca
    12601 gcggttcctc gttgtcgtcg gttaccgagc tgatgcggtg cagaatttct tcggcgacag
    12661 cgtcgagtac gcaacccagg aagttcagga tggcaccggc cgcgtggtcg atcttgcgcg
    12721 cgatttcgtc agcgcatcgc cgtttgtcct gggctacggc gacattctgg tcgacccggc
    12781 gaactatcga tcgattgtcg atcttccgga tgaagtcgaa gcgatcgtca gcgtaacccg
    12841 cgccgaagat gtcagcaaag gcggcgccgt tgcggtgaac gaacgtatgg aagttgtcga
    12901 tattcgggaa aaatcgccgc cgggcgaagc gcccagcccc tggtacaacg ccggtcttta
    12961 cgcatttcgc ccgactattt ttgagttcac tgccaaattg aaaccatcgc cgcgcggcga
    13021 gtacgagttg acggatgcca ttcgcgagct cgcgcaatca ggcaaaagag tgaaagcgct
    13081 cgagctcgtc ggcgaatggg cggatgtgcg cgatccggaa actttggcgc ggctgaatcg
    13141 cacgctgtag cagcatgcct gcctgacgcg ccgtagttgt gcgatggcga ctggaccgtc
    13201 tgcttggacg gcgcacagcg ccgtggctac agtaaaaccg agatgcccgc ttacgtcatt
    13261 gtcgagatcg atatcgtcga tccactcggt tacgacgaat acaagaaact tgccggcgcg
    13321 acagttgaga aatacggcgg caaatacatc gtgcgcggtg gcaaatccga agtactcgaa
    13381 ggcgactggc atccgaagcg catcgtggtt ctccagttcg actccattga acgcgcgaag
    13441 caatggctga attcagagga ataccgcggg ccgcgcaaga tgcgtcatgc gaccgccaag
    13501 accaacatgc tcgtagtcga aggcttttag ccgcggctgc gcccgatagt atcgcgctcg
    13561 ccgcggcgac tgagagcgaa gggctcgaat ccgtgctcgt cggtggaaac gcagtcaatc
    13621 tttacgccta tcgccggacc acgttcgatg tccatcttct cgtgcgcgaa aatgacgcac
    13681 aacgatggct cactttcttt gagcagcgtg gctatcgcgt ctttcatcgc accgacaatt
    13741 tcatccgcat gcatttctcg accgacccga cgaaggcatt gccggtggat ttaatgctcg
    13801 ctgatgacga aaccttccgg aaaatccgcg ctgaaggccg ccgctacgat gtgggcgccg
    13861 gactcgaact tgcgattcca agtccgcttc atctgatcgc gatgaaactt catgccctca
    13921 aaagccaggt gccggtggta tcacgtcctg gaagtcagcg ccacgctcca aactcctcgc
    13981 cgacctcagc ggataagcag actcacaacc atcccggtct accagaccta ccgcaattcg
    14041 attctcttcc ggccaccgct ggccttaaca atgtcgaggc gttccggcta agcattcaac
    14101 acgcgctggc gcttctcccg acaatcctcg caacagcaga cagcttcacc gccgttgcga
    14161 gtcatcctga acggttctcg cttcgataac gcgcgagcgc ctatcgtgat ggagatgcac
    14221 ctgcgaaaat ccaagtttga cataaacgcc tctacaacgt cagcagttct cgcaactgct
    14281 cgggctcggt cacgggtgct ttgcaggtga agttttcgca gacgtaggcg gcgggtttac
    14341 ccttgatcaa cgacatagcg cgaatggctt cattgttttc gccgagatag ttttgtcctt
    14401 cgtcgccatc ggccagtgcg agaattttgt taggcaaaaa atgctgatgc acttctttga
    14461 gcagcagctg cgtgcgcgca ttgttcgcca cggcgaatcc gtcctgcggc ggatcgacct
    14521 tttccgccac gggacggact cgcggtggcg cgccggcaat cacgatttgg cgcggcttgg
    14581 ctaaactgaa gtcgagcgcg accagcatct gcggcatcgc gctcgcgaaa tgcgagagcg
    14641 tcgcggcaaa tgcgcggatt gtttttcgtc cgcggtcggc cagtatctgg tcgtttcgga
    14701 attgcgcgag gcgcaaaaga ttcagcgcgg cgacggagct cgccgccggt tcggcgctgt
    14761 cgttgtcgtc tttcaaccgc agcgggacgc ctgaatcctt ctcgcttgtg ctgaaatagc
    14821 cgccgtgctc tgcgtcgaag aacaagcggt ccagtgtctc ctgcagctcg atggcgaact
    14881 ttagccattc gctgtcgaac gacgcctcgt aaagatcgac aagtccttga atcacgaacg
    14941 cgtaatcgtc cgcaaaccct gcgacatcgc tgcgaccctc gcggtaagtg cgatatagaa
    15001 ttttcgccga ggcatcgtag aggcgttggc gtatgacctg cgccgctcgc gccgcagggc
    15061 cgaggtatcg cgtgtcatcg aggacctgcg cagcgcgcgc ataggccgaa atcatcaacc
    15121 cattccacgc ggcgatgatc ttgtcatccc gatgtggccg cggtcgctta ttgcggatcg
    15181 acgttaactt ttcgcgacta cgcgcgagcg attgctgaac ctcttcctcc gttctcccga
    15241 agcgctgcgc cgtctcagcg attgtgtgac gctcgatcaa aatatttttg ccgcggaact
    15301 cgtcctgcgg gtcacttcct tccggcgcgt tgccgtgtgg ctgcacgccg tagtggaaat
    15361 tgaaaagctc agccgcatca ccgagcgctt tgtcgatttg cttttttgtc cacacgtaga
    15421 acgcgccttc gccgtgctcg ggctttccgt g
//
LOCUS       DASTDG010000121         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_296962, whole genome shotgun sequence.
ACCESSION   DASTDG010000121 DASTDG010000000
VERSION     DASTDG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296962"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VFO30_03865"
     CDS             <1..1026
                     /locus_tag="VFO30_03865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9418455.1"
                     /translation="GFTTVVCMPNTSPAADNPATIAWIKDRAVESACVKVLPSGAISK
                     NLAGEELAPIGSLSKAGVVAITDDGHCVQNHELMRRAVEYARMFDLPLLDHCQDYNLV
                     GNGVVHEGYWSTLLGLQGWPSAGEVAIVMRNILLAELCHHPIHCQHVSSAGSVRLIRE
                     ARKRGVKISGEVCPHHIALTDEAIQNFNTNYKMNPPLRSQADIDALLEGIADGTLSIL
                     CSDHAPHAEFEKEVEFDQAPFGIVGLETELGLFIDLLVHKHRKIDIVRLIEMYTLEPA
                     KLLKLDAGTLSVGAAADVTLIDPNLEWTVRVDQFESLSRNSPFDGWKLKGRTVRTIVG
                     GKTVWQL"
     gene            complement(1084..1539)
                     /locus_tag="VFO30_03870"
     CDS             complement(1084..1539)
                     /locus_tag="VFO30_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9418456.1"
                     /translation="MDVSGHIGAILNQKSGDVYSISPKSTVFEAIQMMADRNVGALLV
                     TEGEQLVGIISERDYTRKVFLRGKSSKETRVEEIMSTNLSVTHPHEGVEECLRRMTDK
                     RIRHLPVIDGDKVVGVISIGDLVKHVISCQSATIAHLENYISGGYTTAL"
     gene            complement(1609..>2502)
                     /gene="sufD"
                     /locus_tag="VFO30_03875"
     CDS             complement(1609..>2502)
                     /gene="sufD"
                     /locus_tag="VFO30_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET9418457.1"
                     /translation="LGSAKFAALHKALVSSGTFLFVPRGVEIELPIEIFHWLRGDNMS
                     LFPHLLLVTDELAKVTVIEHFRSCDRNAPGFACGVNDLIAGPGAKVTYVCAQNWADNV
                     IALQMNTTTVDHDASAMSLNLHLGGRYSRFESLSRLIGEGGRSDLLAVAVATGEQEFD
                     ARTLQDHISPHTASDLLYKNALDDDARCTFGGLIRVEPHAHFADAYQKVRNLLLSDDA
                     EANSMPGLEILADNVRCTHGATSGQIDKDELFYLRSRGIPVPVAQRLVVTGFLNEVIE
                     RLRHPAIAEHLRSLIDAKFAR"
BASE COUNT          548 a          687 c          753 g          514 t
ORIGIN      
        1 ggtttcacga cggtggtttg catgccgaat acctcaccgg ccgcggacaa tccagcgacg
       61 atcgcctgga tcaaagatcg cgccgtggag agcgcgtgtg taaaggtttt gccgagcggc
      121 gcgatctcga agaatctcgc cggcgaagag ctggcgccga tcggttcgtt gagcaaggcc
      181 ggcgtagtcg cgattacgga tgacggccac tgtgtgcaga accacgagtt aatgcgccgc
      241 gccgtcgagt acgcgcgcat gttcgatctc ccgctgctcg accattgtca ggactacaat
      301 ctggtcggca acggtgtcgt gcacgaaggc tactggagca cattgctcgg tttgcagggc
      361 tggccgagcg ccggcgaggt cgcgatcgtg atgcgcaata tcctgctggc ggagctttgc
      421 catcatccca tccattgtca gcacgtaagt tcggccggca gtgtacggct catccgcgaa
      481 gcacggaagc gcggtgtcaa gatcagcggc gaagtttgcc cgcaccacat cgcgttaacc
      541 gacgaggcaa tccaaaactt caacacgaat tacaaaatga atccgccgct gcggtcccaa
      601 gcggatatcg atgcgctgct cgagggaatc gcggatggca cattgtcgat cttgtgctcg
      661 gatcacgcgc cgcacgccga gttcgaaaaa gaagtcgagt tcgatcaggc gccgtttgga
      721 atcgtcggac tcgaaaccga gttggggttg ttcatcgacc tgctcgtcca taaacatcgc
      781 aagatcgaca ttgtccggtt gatcgagatg tatacgctcg agccggcaaa gctgctcaaa
      841 ctggatgccg ggacgttatc ggttggcgca gctgcggatg tgacgctgat cgatccaaat
      901 ctagaatgga ctgttagagt cgatcagttc gaatcgctct cgcgcaattc acccttcgac
      961 ggctggaaac taaagggacg gacagtgcga acgattgtgg gcggaaaaac agtctggcaa
     1021 ttgtagcgac gtttgtgtca aacgccgcat taggggatca ggcgctcgct gcggcgagcg
     1081 cctctacaat gccgtggtat agccgccgga gatgtagttt tccaaatgcg caatggtcgc
     1141 gctctggcag gaaatgacgt gtttgaccag atcgccgatg gaaatcacgc cgacgacttt
     1201 atcgccatcg atgacgggca gatggcgaat gcgtttgtcg gtcatacggc gcaaacattc
     1261 ctccacacct tcgtgtgggt gggtcaccga taggtttgta gacatgatct cctccacgcg
     1321 cgtttccttc gacgacttgc cccgcaggaa aacttttctc gtgtaatcac gctcggaaat
     1381 aatgccaacc agctgctcgc cttccgtgac cagcaatgcg cccacattcc tgtcggccat
     1441 catttgaatc gcctcaaaca ccgtgctctt cggcgaaatc gagtaaacgt caccgctttt
     1501 ctgattcagt atggcgccga tatgcccgct gacatccatg ggaaagaaat tgcaaaatac
     1561 accagggcaa acggccgtgg caaaagaatt ctgtcaggcg cgcagaaatc agcgggcaaa
     1621 tttcgcgtcg attaagctgc gcaggtgttc cgcaatggcg gggtgtcgca gccgctcaat
     1681 gacctcgttc aaaaaacctg tgacgacgag acgttgcgcg accgggactg gaatgccgcg
     1741 ggagcgcaga tagaagagct cgtctttgtc aatctggccg gaggttgcgc cgtgggtaca
     1801 gcggacgtta tcggccagga tttcgagtcc gggcattgag tttgcctctg cgtcgtcgct
     1861 gagcaggaga ttgcgaactt tttggtaggc atccgcgaaa tgcgcgtgcg gttcgacccg
     1921 gataaggccg ccgaaggtgc agcgagcgtc gtcatcgagc gcgtttttgt aaagaagatc
     1981 gctcgccgtg tgcggcgata tgtgatcctg caaggtgcgg gcgtcgaact cctgttcgcc
     2041 cgtggcgacc gcgacagcga ggagatcact gcgaccgcct tcgccgatca accggctcag
     2101 gctctcgaaa cgggagtacc gcccgccgag gtgcagattg agactcatgg cggaggcgtc
     2161 gtgatcgaca gtcgtcgtgt tcatttgcaa cgcgatcaca ttgtccgccc agttttgcgc
     2221 gcaaacgtaa gtcaccttcg caccgggccc ggcgatgaga tcgttcacgc cgcaggcaaa
     2281 tcccggcgcg ttccggtcgc acgagcggaa atgctcgatc accgtaactt tggcgagctc
     2341 gtctgtgacc agaagcaaat gcggaaagag cgacatgtta tcgccccgca accagtggaa
     2401 gatttcaatc gggagttcga tttcgacgcc gcgaggcaca aacagaaatg tgccggacga
     2461 aacgagcgct ttgtgcagtg cggcgaattt ggcggacccg ag
//
LOCUS       DASTDG010000122         8845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_262069, whole genome shotgun sequence.
ACCESSION   DASTDG010000122 DASTDG010000000
VERSION     DASTDG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262069"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFO30_03880"
     CDS             <1..239
                     /locus_tag="VFO30_03880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET9418458.1"
                     /translation="FAVHLDHGDEEPCYDCIASGFYSSVMIDASHEPFKENGAITKRV
                     VDKAHGKGISVEAELGMLGGVEEDIVRKSRLLSK"
     gene            236..1252
                     /gene="lpxD"
                     /locus_tag="VFO30_03885"
     CDS             236..1252
                     /gene="lpxD"
                     /locus_tag="VFO30_03885"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET9418459.1"
                     /translation="MTFTLQELATLSGGELIGDPAQKITGAASLAEATDSEISFFGNP
                     RYIAQLRKTRAAAVFVPSDFSEQTPAAQIRVSNPAKAFEQIMLKLAPKPIAFSPGVHQ
                     TAIVDQSARLGVGVSIQPYAVVEAGVSIGDGTLIGAGVYIGHETVIGKNCTIYPRVTI
                     RERTRIGDGVVIHSGAVIAADGFGFESSQGRHEKVPQLGIVQIDDDVEIGANTTIDRA
                     RFGRTWIQTGAKIDNLVQVAHNVVIGKNTIIAAQTGIAGSVRIGNNVLIGGQAGIIGH
                     IEIGDNTAIGAQSGVSKNISGGAWWMTPATPLPEVKEQMAWVRRLGKLFERVTAIEKK
                     LGLR"
     gene            1332..1763
                     /locus_tag="VFO30_03890"
     CDS             1332..1763
                     /locus_tag="VFO30_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418460.1"
                     /translation="MPPFRDSYQFLIIDDDPAITRFLVTYLRQKGHSCQALTEGFQTA
                     TWLAHNNCEVAIVDLRMPKVDGISLISYIREINPAIPIVVFTGIGYDEEKMHAALRAG
                     ANGYVSKNLPIEQLYCVLARVLATSREQMNRAAYAVAAGAA"
     gene            complement(1774..2193)
                     /locus_tag="VFO30_03895"
     CDS             complement(1774..2193)
                     /locus_tag="VFO30_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9418461.1"
                     /translation="MQQRSHYLIVDGHSVIFAWPELRKLHERRSALAREALIKQLRDY
                     QDWSGVRVVVVFDGRGSRVSVSAERGDIQIFYSRQGQTADAIVERLAGKYAGRLDVVV
                     ATADAKEQATVSSFGASCVSTDGLRDLLRQARSRELR"
     gene            2548..3354
                     /locus_tag="VFO30_03900"
     CDS             2548..3354
                     /locus_tag="VFO30_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418462.1"
                     /translation="MSTLLLVLAAFFGVLNTFRVSALQTNLARTAAARDAAETRRAAL
                     EQPRGTRGATAPAATPRTADERNAAKAEADLAQVLKEKADLQTKLKATEAELASVKNQ
                     PQETGAESAGVNPGAASTAELQAQLDDTRRQLENAERENALLSQKLGGAPTIAASEAT
                     ATTSASSAVAAPQTKVKRREPSPAKARLRGTVMAVNQAYNFVVLNLGERQGLRSNEDM
                     LVLREGVLIGKLRISSVEPATAIGDIVTNSLARGVQVQPGDVVIYEDTNR"
     gene            complement(3588..4883)
                     /locus_tag="VFO30_03905"
     CDS             complement(3588..4883)
                     /locus_tag="VFO30_03905"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET9418463.1"
                     /translation="MAPLSLPPGTTQRTLAIIMGGGAGTRLFPLTKDRAKPAVPLGGK
                     YRLVDIPISNCLNSGLRSIYVLTQFNTMSLHRHIQASYKFDNFSRSFIDILAAQQTPE
                     RAQWYQGTADAVRQNMRYFLERPFDYYLILSGDQLYRMDFRVLLHQHIQSDTDITLAT
                     KPVRREDVSEFGIMQSGADRRITRFVEKPQDETVLHEMRMSRELLATTAASPDEELFQ
                     ASMGIYIFNRDVLVKCLANELVDFGKDVIPQAIKDRHVSAFIFNGYWEDIGTVRAFYK
                     ANLDLTDLVPEYSFFESETPIYTHPRFLPGSKVNGATLRQAIISDGCIISDAHLERSV
                     IGIRSIIQSGATIRNSVVMGADYFEQDRHEKAGHIPIGIGRNCVIDRAIIDKNARIAD
                     GVVITPEGKLPNLDTENYYIRDGIVIVPKNAVIPPGFWV"
     gene            5032..6891
                     /gene="glmS"
                     /locus_tag="VFO30_03910"
     CDS             5032..6891
                     /gene="glmS"
                     /locus_tag="VFO30_03910"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9418464.1"
                     /translation="MCGIVGYVGRAEAAPILLEGLRRLEYRGYDSAGIAVVNGHNQFE
                     TRKCAGRIASLAKLIARDPASGRVGISHTRWATHGRVNDENAHPHFDATGKIALVHNG
                     VIENYQALKEQLIQNGDTNFRSDTDTEVLAHLIGRIYQQLDGKDSKARLVNAVRAALK
                     QVIGTYGIAVVHADVPEFIVGARRGSPLVLGIGREENFLASDVSAIVAYTRDAVYLSD
                     FEIVALDPERFEITSLDGATEQHTVSRVEFTAEDIKKGDYPHYMLKEIFEQPSSVRDA
                     MRGRLSFEDCTAKLGGLNTIPAELRKICRIVLTGCGTAFHAAMVGEYLIEQLASIPTE
                     VEYASEFRHRNTPMTPDTLVFAISQSGETADTLGALRESRQKGYRTLGICNNVASTIA
                     RESDGGVYMHAGPEIGVAATKSFTSQLVILTLIGLLLGRMRNLSTAQGARIIDALENL
                     PDKVQETLKLSDQIKALARKYVDVSGVLFFGRQFSFPIAMEGALKLKEITYLYAEGHP
                     SAELKHGIIALIRPDLPSIFIAPDDAVFSKNLNNIEQVKARKGPIIALTSGNGASQLK
                     GIADDIVTLPSVPDCLMPILTVIPLQLLAYHLAVELGRDVDKPRNLAKSVTVE"
     gene            complement(7009..>8845)
                     /locus_tag="VFO30_03915"
     CDS             complement(7009..>8845)
                     /locus_tag="VFO30_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9418465.1"
                     /translation="AASNDANGVFDFVGSDGTPNTFFSSGGSLARFNGFRYLKYKALL
                     STTNSSVTPVVNDVTVCFNNSPGGPTPTPTPTPTATPTPTPTITPTPTPTPKQDQTIT
                     FPPISTKTYGDADFDPGATASSGLAVTYTTSGQCTIVSNKVHITAAGSCIVTANQSGN
                     GQYNPARPVSQSFSILKAGTTTTSSLLANSPNPTPQFSDTAKLTATVKANTTVTGNGV
                     FSGTVSFTFNGVAVGSAITVSNSSPTAQLSLKLDQTRIPLGAGNYNINATFTPSASSN
                     YGASTSGAHAVAVRHEGQKADYTGDGSARLDYTGTQYALLGSAPTLSAMLRQSLAPDV
                     EPEFVDFSKVNTYVVFKIVPAACGTSCSDTPVWQSANIKINNRADWSTTGIGTAQVTA
                     PTTLGERSYLVLVNVVTNGYILAERAAATLDVFSTTGRFITGGGSVTTDSTSNAGTRR
                     GYFGFDVKGTTTVSGSSIYLYRLRMNVSTSTATNIVTCSTMGNGCTDVDIILRSNAIS
                     SLNTGTSLTYPLTGFTAGHAAAQFVNAYSGGHYTQFEFANGFFRLDILDNSSGGSTDK
                     YGFTAYRGNGSIFHQAYIGPIAQTGILAKTNEITIGGGNTTVHPK"
BASE COUNT         1886 a         2506 c         2617 g         1836 t
ORIGIN      
        1 tttttgccgt gcacctcgac cacggagacg aggagccttg ctacgattgt atcgcatcag
       61 gcttttacag ctcggtcatg atcgatgcca gccacgagcc atttaaggaa aacggcgcca
      121 tcaccaagcg tgtcgtcgac aaggcgcacg gcaaagggat cagcgtcgaa gccgagttgg
      181 gaatgctcgg cggagtggag gaagacattg tccggaaatc tcgcctgctt tccaagtgac
      241 gttcacgctt caggaactcg cgactttgtc tggcggcgag ttgatcggcg atcccgcaca
      301 aaaaatcact ggcgcggcct cgctcgcgga agcgactgac agcgagatct cctttttcgg
      361 aaatccccga tacatcgcgc aactacggaa gacgcgcgcc gccgccgtgt ttgtgccgtc
      421 cgatttctcg gaacaaactc cggccgcgca aatccgtgtc tcgaatccgg caaaagcgtt
      481 cgagcaaatc atgctgaagc tcgctccgaa accgatcgcg ttttccccgg gcgttcatca
      541 aactgcgatt gtcgatcaaa gcgccaggct cggcgtcggc gtgtccattc aaccgtacgc
      601 ggtcgtcgag gctggcgtga gcattggcga cggcactctg atcggcgcgg gcgtttatat
      661 cggtcacgaa accgttatcg gaaagaattg cacgatttat ccgcgggtca cgattcgcga
      721 gcgcaccaga attggtgatg gggtcgtaat tcacagcggc gcagtgatcg cagccgacgg
      781 cttcggcttt gaatcttcgc aaggccgcca cgaaaaagtt ccgcaactcg ggattgtcca
      841 gatcgatgat gatgtcgaga tcggtgccaa caccacgatt gaccgggcac gtttcgggcg
      901 gacatggatt cagactggcg cgaagatcga caatctcgtg caggtggcac acaatgtagt
      961 gattggcaaa aacacaatta tcgctgcgca gaccgggata gccggcagcg tgcgcatagg
     1021 caataacgtg ttgatcggcg gtcaggccgg gatcattggc cacatcgaaa tcggcgacaa
     1081 taccgcgatc ggcgcgcaga gcggcgtctc aaaaaacatt agcggcggcg cttggtggat
     1141 gacgccagcc acgcccctgc cagaggtgaa agagcaaatg gcctgggtgc ggcgcttggg
     1201 caaacttttc gagcgggtga cggcgatcga aaagaagctc ggactgcgat agagaaacgc
     1261 ctgcgctcgg cgccgcggac tcgtggttgg caagcttagt gctatacagc tgttctagta
     1321 attatagcgc catgccgccc tttcgcgatt cttatcaatt cctcatcatt gatgatgacc
     1381 ccgccatcac ccggttcctc gttacctacc tgcggcaaaa aggccacagc tgccaagcgt
     1441 tgaccgaagg gtttcaaacc gccacctggc tcgcgcacaa caattgcgaa gtggccattg
     1501 tcgatcttcg catgccgaaa gtggacggca tttcgctcat ctcgtacatc cgcgaaatca
     1561 atccggcgat ccccatcgtg gtctttaccg gcattggcta tgacgaggag aagatgcatg
     1621 cggcgttgcg tgccggcgcc aatggctatg tcagcaagaa tttgccgatt gagcaactct
     1681 actgtgtgct ggcacgcgtg ctggcgactt cccgtgagca gatgaatcgc gccgcctacg
     1741 ctgttgctgc gggagcagct taagcgtttg ggctcaacgc agctcacgcg agcgcgcttg
     1801 tcgcagcagg tcgcgcaaac catccgtcga aacgcagctc gcaccgaagg aactgacggt
     1861 ggcctgttcc ttcgcgtcgg ccgttgccac taccacatcg aggcggcccg cgtacttgcc
     1921 tgcgaggcgc tcgacaattg cgtcggcggt ttggccctgg cgggaataga atatctggat
     1981 gtcgccgcgt tccgcactca cgctcacccg cgacccgcgg ccatcgaaaa caaccactac
     2041 tctcactccg ctccaatcct gataatcgcg cagttgcttg atcagcgcct cgcgcgcgag
     2101 cgccgaacgg cgttcatgca atttacgcaa ctccggccaa gcgaagatga cactatgacc
     2161 gtcgacgatg agatagtgtg atcgctgttg cattgctcgc cgctctcggc cagcgaacca
     2221 ccgacgccga aaatcctatt ctggtggcgc gcttggctgc cttttgttct ttgcccgtag
     2281 cggttcgcgc cgcgccgctt tgagcgcgtc ctttttgcgg cggagatatg cctgtcgccg
     2341 cttgcgcttc gcgcgaaccc ggtgctgctg gcccatgccc ggttataaat ttgaacttcg
     2401 cctggcgcaa cgtgaatccc tgtcgatttc gcgacaggcg gtaaacgaaa tcgagcattg
     2461 cattgtccaa tcgtggtcga ttacaagata ccggccttgg cccccacgtt catgactttc
     2521 acctccactc cgaagactgt gctcagcatg tcaactttgc tgctggtgct ggccgcattt
     2581 ttcggcgtgc tcaacacctt tcgtgtcagc gcgctgcaga caaatcttgc gcggacagca
     2641 gcggcacgcg acgcagccga gacgcggcgc gccgcgctcg agcaaccacg cggaacccga
     2701 ggcgcgacgg cgccagccgc cactcccaga acggccgacg aacgaaacgc cgccaaagcc
     2761 gaggctgatc tcgcccaagt gttgaaagaa aaggcagacc tgcagacgaa acttaaagca
     2821 accgaggccg aactcgcttc agtgaaaaat cagccgcagg aaacaggcgc tgaaagcgct
     2881 ggcgtcaatc caggcgcggc atcgactgcc gagttgcagg cgcaattaga cgacacgcgc
     2941 cggcagctag agaacgctga gcgggaaaac gcgctgctgt cgcaaaagct cggcggcgca
     3001 ccgacgatcg cggcgtcgga ggcaactgcg acaaccagcg cctcttctgc cgttgcggct
     3061 ccccagacaa aagtgaagcg gcgcgaacct tcaccggcca aagcccgcct gcgcggtaca
     3121 gtcatggcag tcaatcaggc gtacaatttt gtcgtgttga atctgggcga gcgacagggt
     3181 ctgcgcagca atgaagatat gctcgttctg cgcgagggcg ttttgatcgg caagcttcgg
     3241 atttcctcgg ttgaaccggc caccgcgatt ggggatattg tgacgaacag cttggcgcgg
     3301 ggcgtccaag tgcaaccggg agacgtcgtg atttatgagg acactaatcg ctaaactgcg
     3361 cagacatgtc tgcctcgccc tactggtcac gagcacagct ggctttgtct cctgtgcgtc
     3421 acaacgcgat caggctcggc tcgtcgaaga tccggatgct aaacactacg actctgccat
     3481 tccctggaac cgacaggaga aatgggagat gggcgcgcaa ctgtcaggaa tgatcgaccc
     3541 gcgcagtcag tgacgaagca accgtcgctt ccagcaaaaa gcgacgccta aacccaaaag
     3601 cccggcggaa tgacggcgtt ttttggcacg atgacgatgc cgtcccggat gtagtaattt
     3661 tccgtgtcga gattcggcag ttttccttct ggcgtgatca cgacgccatc ggcgatgcgc
     3721 gcatttttgt cgataatcgc gcgatcgatc acacaattac gaccgatgcc gatggggatg
     3781 tgccccgcct tttcatgccg atcttgctca aaataatccg cgcccatcac aacgctgttg
     3841 cgaatcgtgg cgccgctttg aatgatgctg cggatgccga tgacgcttcg ctcgaggtgc
     3901 gcatcggaaa tgatgcaacc gtccgaaatg atggcctggc gcagcgtcgc gccgttgact
     3961 ttgctccccg gtaaaaaacg gggatgcgtg taaatcggcg tctccgactc gaaaaaactg
     4021 tactccggca cgagatcggt caggtcgaga ttggctttgt aaaacgcgcg caccgtcccg
     4081 atgtcttccc aatagccgtt aaaaatgaat gcgctgacat gtcgatcttt gatcgcctgc
     4141 gggatgacgt ccttgccaaa atcgacgagc tcatttgcca ggcatttcac cagcacgtcc
     4201 cggttaaaaa tgtaaattcc catcgaggcc tgaaacaatt cctcgtcggg gcttgcggcc
     4261 gtggtggcga gcagttcgcg gctcattcgc atttcgtgga gcacggtttc atcttgcggc
     4321 ttctcaacga atcgcgtgat gcgccggtca gcgccggatt gcatgatgcc aaattcgcta
     4381 acgtcctcgc ggcgcaccgg cttggtcgcc aacgttatgt cagtgtcgga ctggatgtgt
     4441 tgatgcagca gcacgcggaa atccatccga tacagttggt cgccgctcag gatgagataa
     4501 taatcgaacg gccgctcgag gaagtagcgc atgttctgtc gcacggcgtc agcggtgccc
     4561 tgataccatt gcgcacgctc gggggtttgt tgcgcggcga gaatgtcgat gaagctgcgc
     4621 gagaaattgt cgaacttgta gctggcttga atatgccgat gtaacgacat cgtgttgaat
     4681 tgcgtcagca cgtagatcga gcgcagaccc gagttcagac agttgctaat cggaatgtcg
     4741 acgagacgat attttccgcc gagcgggaca gccggtttcg cacggtcttt ggtcaatgga
     4801 aacaagcgcg tccccgcgcc gccgcccatg atgatggcca gcgtgcgctg agttgtgccc
     4861 ggtgggagcg agaggggcgc cacggctggg ttcgccgctg ctgaatcgcg ccgcatcgct
     4921 tctcctgtta tcacaggcgc tactctatcg cagcggtgtg atgattccgg caactttttt
     4981 gcgcacggcg cgcatcttgc ggctgaggag agccgcccct acaatttcga tatgtgcgga
     5041 atcgtcggtt atgtcgggcg cgccgaagcg gcgccgatcc tgctcgaggg attgcgtcga
     5101 ctcgaatacc gcggctacga ctctgccgga atcgctgtcg tcaatggcca caatcagttt
     5161 gaaacacgca aatgcgccgg acgcatcgcg tcgctggcga agttgatcgc ccgcgacccg
     5221 gcctcgggtc gcgtcggcat cagccacacg cgttgggcca cgcatggcag ggtaaacgac
     5281 gaaaacgccc atccgcattt cgatgctacc ggcaagatcg cgctcgttca caacggggtg
     5341 atcgagaatt accaagcgct taaggagcaa ctgatccaaa acggtgatac caactttcgc
     5401 tcggacaccg acaccgaagt tttggcgcat ttgattgggc gcatttacca gcaactcgac
     5461 ggcaaagatt cgaaggcgcg gctggtgaat gcggtgcgcg cggcgttgaa gcaagtcatc
     5521 ggcacttacg gcatcgcggt tgtgcatgct gacgtgcctg agtttatcgt cggtgcacgc
     5581 cgtggcagcc cgctggttct gggaatcggg cgcgaggaga attttctagc gagcgacgtg
     5641 agcgcgatcg tcgcctacac gcgggacgcc gtttatctca gtgacttcga aatcgtcgcg
     5701 ctggacccgg agagattcga aattacctcg ctcgatggcg cgacggaaca gcacacggtc
     5761 agtcgcgtgg agtttaccgc ggaagacatc aaaaagggcg actacccgca ttacatgctc
     5821 aaggaaattt ttgagcagcc ctcttccgtc cgcgatgcga tgcgcggtcg gcttagcttt
     5881 gaagattgca cggcgaagct cggcggcttg aacacgattc cggccgaact gcgcaagatc
     5941 tgccggatcg tcctcaccgg ctgcggcacc gcctttcacg ccgcgatggt cggcgaatac
     6001 ctgattgaac agttggccag catccccacg gaagtggaat acgccagcga gtttcgccac
     6061 cgcaatacgc cgatgacacc ggacacgctg gtgtttgcga tcagtcaaag tggcgaaacg
     6121 gccgacacac tgggcgcgct ccgcgaaagc cgacagaaag gttatcgcac ccttggaatt
     6181 tgtaataacg tggcgagcac gatcgcgcgg gaaagcgatg gaggcgttta catgcacgca
     6241 ggtccggaga tcggcgtggc ggcgaccaag tctttcacat cgcagctggt catcctcaca
     6301 ttaatcggcc tgcttcttgg gcgaatgcgc aacctttcca ccgcgcaagg cgcgcggatc
     6361 attgatgctc tcgagaatct gcccgacaaa gtccaggaga cgctcaagct cagcgaccaa
     6421 atcaaagcgc tcgccaggaa atatgtcgat gttagcggag tgcttttctt cggccggcaa
     6481 ttcagtttcc cgatcgcgat ggaaggcgcg ctcaagttga aagaaatcac ctatctttat
     6541 gccgaaggac atccgagcgc cgaactcaaa cacggcatca tcgcgctgat acgccccgat
     6601 ctgccatcga tcttcatcgc tccggacgac gcggtcttct caaagaacct caacaacatc
     6661 gagcaagtaa aagcgcgcaa aggtccgatc attgcgctga cgagtggcaa cggcgcaagc
     6721 cagctaaaag gcatcgccga tgacatcgtc acgcttccca gcgtccccga ttgcctgatg
     6781 ccgatcctca ctgtaattcc gttgcaactt cttgcctatc atctggccgt cgagctcggt
     6841 cgcgacgtcg ataagccgcg caacctggcg aaaagcgtca cggtggaata ggttttccaa
     6901 acggcggcgg ctaatcggct cggtggcggt cgcagatcgg cgcgacaaaa agaaggccgg
     6961 cgatgtcatc gcccggctcg ggtaggttcc tctctctcag gttgtggttt acttggggtg
     7021 cacggtggta ttcccgccgc cgatcgtgat ttcattcgtt ttcgccaata tcccggtttg
     7081 cgcgatgggg ccgatgtacg cctggtggaa gatcgacccg ttgccgcggt aggcagtgaa
     7141 accatacttg tcggtcgaac cgccggagga gttgtccagg atgtccaacc gaaagaatcc
     7201 attggcgaat tcgaattgcg tgtaatggcc gccgctgtac gcattcacaa actgagcggc
     7261 ggcgtggccg gcggtaaacc cggtcaacgg gtaagttagg cttgtgccgg tattgagcga
     7321 actaatggcg ttactacgca aaatgatgtc gacgtccgtg caaccattac ccatcgtgct
     7381 gcacgtgacg atgttggtgg ccgtgctcgt gctcacgttc atacgcagcc ggtagagata
     7441 aatcgaactg ccactgaccg tggtcgttcc tttcacgtcg aacccgaaat acccgcgcct
     7501 cgtgcccgcg ttggaagtcg aatcagtcgt gacggagccg ccgccagtga tgaatctgcc
     7561 ggtagtcgaa aagacatcga gggtcgcggc tgcacgctcg gcaaggatgt agccgtttgt
     7621 cactacattc acgagcacga gataacttct ctcgcccaat gttgttggag ccgtgacctg
     7681 agcggtgcca attccggtcg tgctccagtc ggcgcgattg ttgattttta tgttagccga
     7741 ctgccagacc ggtgtgtcgg aacagctcgt gccacacgct gccggaacaa ttttgaaaac
     7801 gacgtaggtg ttaacttttg aaaaatcgac aaactccggt tcaacatcag gcgcaaggct
     7861 ttgccgcaac atggcagaga gagttggcgc ggaaccgaga agcgcgtact gcgtgccagt
     7921 atagtcgagt cgcgcggagc cgtcgccggt atagtccgcc ttttgacctt catgcctaac
     7981 ggctacggca tgcgcaccgc tcgtgctcgc gccataatta ctgctcgcgg agggcgtgaa
     8041 cgttgcgttg atgttgtagt tgccggcgcc gaggggaatt cttgtttgat caagtttgag
     8101 cgaaagctgt gctgtcggcg aggaattgct gacggtgatc gcgctaccaa cagcgacacc
     8161 gttgaaggta aagctaactg ttccggaaaa cacgccattg ccggtaacag tggtattagc
     8221 cttgactgtg gcggtgagct tggccgtatc gctgaactgc ggcgtgggat tcggcgaatt
     8281 agccagcaac gaagacgtgg tggttgtgcc tgccttgagg atgctgaacg actgtgacac
     8341 gggcctggct gggttgtact gaccgttgcc cgactgattc gcggtcacaa tgcatgagcc
     8401 ggcggcagta atgtggacct tattcgaaac aatcgtgcat tggcccgagg tggtataggt
     8461 caccgctaag cccgagctcg ccgtcgcacc ggggtcaaaa tcagcgtcgc cgtatgtctt
     8521 ggtggaaatt ggcgggaacg taatcgtctg gtcctgcttg ggagttggag taggtgtcgg
     8581 ggttattgtg ggagtaggag ttggtgtagc tgtgggagtg ggtgttggag tcggtgtcgg
     8641 cccacccggt gagttattaa aacaaaccgt gacgtcgttc acgaccggag tgaccgaact
     8701 gttggtcgtg ctcaataatg ctttgtactt gagataccgg aaaccgttga agcgcgcgag
     8761 cgagccgccg ctgctgaaaa atgtgttcgg cgtgccatcg gagccaacga agtcgaacac
     8821 gccgttagca tcgttgctcg ccgcc
//
LOCUS       DASTDG010000123        10452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_385857, whole genome shotgun sequence.
ACCESSION   DASTDG010000123 DASTDG010000000
VERSION     DASTDG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385857"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2854)
                     /locus_tag="VFO30_03920"
     CDS             complement(212..2854)
                     /locus_tag="VFO30_03920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9418466.1"
                     /translation="MRRWLVLAPILFLFVGKMAAEKPFDFATTPGKLPKQVVPSEYAV
                     RVEPNLEKLTFTGSETIKLEVHAPVRELVLNAADIAIRSATLDGKTVPANALKIDTQN
                     ELLRIALASELAPGKHELRLSFSGKVNQQGRGLYYMPYQEEGTGAKKTALGTQFEATD
                     ARRFFPCWDEPSFRARFQLTAVIPENWTAVSNMPVELERKIGNNRKEVRFALTPSMSS
                     YLNVFVAGELDMIATKAGTELRVVTTKGKAGTGRYALESTAQILDYYNDYFGVPYPLP
                     KLDQIAIPGGFGGAMENWGGITYFETALLFDPQKSSESTRQIIYEVIAHEVAHQWFGD
                     LVTMGWWDNLWLNEGFASWMGSKCTARFNPQWEVWLQKVTPRDPTRRTGISKELEMEG
                     DARSTTHPIQQPITNEAEANSAFDDITYRKGQAFLVMLESFLGEDAFRDGIRRYIAAH
                     KYSNSTTADLWKALREASGQPVEEIAAGWTEQPGFPVLKVKRDGEKVSLSQERFTIHY
                     PDAPPLEWKIPLTYFVVGDTKSQSRLMTGKIDNLPNIAADGVLKLNADGAGNYRVEYD
                     ARSWKLLLNALPKLGVPDRVNLLGDAWAFAQADRAPISQYLELVGKLPANIELAEREQ
                     IINTFDYINRLLIGDPVREKFQSYAHSILRPSFDQLGWSPKKDEPPRDAILRGSLIEA
                     LGALGDETTIAGCRERFQKYLSEPASLTPDLRWPVFWVVGHYGGENEWNTLHELGLKT
                     TSSEEQENYYDALTPVRDPKLIARTLQIALTDELPTSRAVFLVGKVARYAERPDLAWD
                     FAKANMKALLAKIDALGANSYAPSLFTFFSDPARATELREYAKTNLPVTAAKDVAKAV
                     DEIEFRAEFKKRLAAQLANSLQKR"
     gene            complement(2889..3332)
                     /locus_tag="VFO30_03925"
     CDS             complement(2889..3332)
                     /locus_tag="VFO30_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418467.1"
                     /translation="MQTRRLWFVTLFALVMSAAGSLHAAHTVWSGLVIAQNVAQPAPI
                     PPALMRIEETLKELFGYNQFQVIGQSTKTLKTGEEDWLASSKYFGLHVDARGETSAGY
                     LVDLKLYKEEDLLLETETKLSKRSPLVIKGPQVGNGQLLLILVVQ"
     gene            complement(3339..3851)
                     /locus_tag="VFO30_03930"
     CDS             complement(3339..3851)
                     /locus_tag="VFO30_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418468.1"
                     /translation="MKTFEEKWTAWLDGELTGKELEEFEASLPDRAAAEAEKRAAHKL
                     GVLLKQELGTEVLNNEEFFSHQIRERLARDACAQDRHRTRSTWWTVPRLALSGAVSLA
                     LFLVCAVFVMHDKNPAEQSQYLTQILNARVDAAVSPNATISIFETKEDKVTVLWVEGL
                     QSLPADYAAK"
     gene            complement(3838..4449)
                     /locus_tag="VFO30_03935"
     CDS             complement(3838..4449)
                     /locus_tag="VFO30_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9418469.1"
                     /translation="MQTEATLAQADVSELELVKRCQAGRTDAFDELVVRYRTRVFAMI
                     YNMVHNEQDAWDLAQESFVKAWKSIKRFRRHSSFYTWIYRIVMNVTIDWLRKKQVKGT
                     GAEFDDSIQLKEVDPASRTLPKADPLPHERMQRGEIREKIDNAIKQLSPEHRAVILMK
                     EIEGMQYHEIAETLGCSIGTVMSRLFYARKRLQNLLRDVYENI"
     gene            complement(4488..5147)
                     /locus_tag="VFO30_03940"
     CDS             complement(4488..5147)
                     /locus_tag="VFO30_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418470.1"
                     /translation="MAEYDPESDKIWDEYDWERFLQQQDQKTEKYMELLEKYLDDPQR
                     DEIIAREMGWSQFAEDNDWSEEVDALAKEDWCSGADLADEDANAIRLESSENHSLYRA
                     AFALTVWIDQLFDAEAALQNEPAAIKLATHSALASAKLAAALNDDDLDEIGMTIAYLK
                     RALKAITTSFEAAAQLLKGRHITVDQHSILQKRLFEVRDGIISLMGEYRGEWRRRFGS
                     R"
     gene            complement(5213..6304)
                     /gene="rlmN"
                     /locus_tag="VFO30_03945"
     CDS             complement(5213..6304)
                     /gene="rlmN"
                     /locus_tag="VFO30_03945"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9418471.1"
                     /translation="MPEPAKIDLKSLGIEELQQRLCASGEPAYRVGQIADWLYKKRAQ
                     SFQEMTDLPRNLRERLEREFGFARIEIVRVLGSSDTTRKFLFQLADGNLIESVLIPAS
                     PALYGTHADRRTICLSTQVGCAYGCKFCASGLEGFSRNLRANEIVDQIIAVERATAEK
                     IDNIVFMGMGEPLANLENVMRAIRIMNAPWGLGIGARHITVSTSGLVPQIRKLAEEPL
                     QIRLAISLHGATDEVRNQIMPINRRYNIDMLLAACHYYAARKKQRLTFEYILIAGIND
                     ALEQARLLVQHARRVSAKVNLIPYNTVVGLSWSRPSRAWQEKFLSILRSAGVAATLRR
                     EKGHDIEAACGQLRLQTKKVEQASGLLLQ"
     gene            6588..7349
                     /locus_tag="VFO30_03950"
     CDS             6588..7349
                     /locus_tag="VFO30_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418472.1"
                     /translation="MQKSRNPLALGWEAARANAAPALVIQLMMLALLVAYYTSPAAAS
                     ALSRVAEFKRAHGIVFVVSASIAAGALIPEIFLILFFQHGRPSSRNLRNLLFTVPVWG
                     LDGSLVDLLYRSEAHWFGDVATLPVVGAKICVDQFGYNPFFAAPFGVLTYEWKNSGIS
                     WRPVRDLFTWPHYRDKIVPTLCATWAMWIPLMAMIYSLPLVLQFPLFGLALSFWVLLL
                     TYMTNRFAAKIEADAPLALAVTEATERAELTSGAR"
     gene            complement(7330..8112)
                     /locus_tag="VFO30_03955"
     CDS             complement(7330..8112)
                     /locus_tag="VFO30_03955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9418473.1"
                     /translation="MSIRLLSIDFDGTLVSRVSEPVLDAACIDLIRELQTSGVQFAIN
                     TGRSVDLLESGLTDFSIPLRPDFILTSERHVFRPGQNGDKWEAFGDWNERCARDHTEL
                     FRSADSVLAEIVDFVNQKTKARLIYQSDGLEGLVASNDEEMNRIVKFIERAREQQPNF
                     HYQRNTLWLRFCHANYHKGAALAELARLIDVPRENIFAAGDHHNDVSMLDGKIAGMPA
                     CPANAIVEVKLAVQSAGGYVANKEHGAGVHEALEFFLNGHRK"
     gene            8378..8665
                     /locus_tag="VFO30_03960"
     CDS             8378..8665
                     /locus_tag="VFO30_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418474.1"
                     /translation="MKSAYELAMERLEKNQPSVALTAEQKQQIAEIDSSYKAKIAEKE
                     LLLRDEIRKAQTAGNFEEAEKIEQQLASEVKRLRDDCDAKKEKLRASFVTK"
     gene            complement(8698..9663)
                     /locus_tag="VFO30_03965"
     CDS             complement(8698..9663)
                     /locus_tag="VFO30_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9418475.1"
                     /translation="MNTDVIRVERPTTNSQLFAHTRWDAVPALAGLFHLGYFFALFWL
                     YPRTPLWLMLILGLVYSLMINANINGVGHNFIHNPFFRSNILNRLFGVTQSIACCFSQ
                     TYYDLVHMQHHKGNADRPDEKGETVDWISIYRHGDHGEPENPWSYVFLSFFRDDVGLI
                     RKELRKRNNGDLTWGNIELAAFAATLLILFCFNWRYVIFYFLPFWYLGHCFSFLNGYY
                     RHYGGNPDNPVAWGVSSYGKLYNWLFFYNGYHAEHHFRPKVHWTKIEAFRRQIADLQK
                     QEGTRVIKRAHMLGFLDPDLPPRGKGQSAAPRAMSEQMAASPRNL"
     gene            9764..>10452
                     /locus_tag="VFO30_03970"
     CDS             9764..>10452
                     /locus_tag="VFO30_03970"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9418476.1"
                     /translation="MFALQPEMKIFSGSANRGLAERICRYIGVPLGQATLSSFPDGET
                     YVKIEENVRGRDVFLIQPTSPPTNEHLMELLIMVDAARRASADRITAVIPFFGYARQD
                     RKDKPRVPITAKLIANLLHAAGVNRVLTMDLHAQQVQGFFDIPVDHLYSLPVLIRYLR
                     KRLTGKTVVVSPDIGGLKMASAYAEALGAGLAIVAKERKSATETEALNLMGEVEGHDV
                     LLVDDLTETAGT"
BASE COUNT         2153 a         2949 c         3054 g         2296 t
ORIGIN      
        1 cgctgctgac cgaccgcccg gaaaacatgg cacaagagtt ttttcggaaa cacggattcg
       61 tcgagtcctc gatgatcccg atgcggttat ggatcacgcc gcagccggac caagcgcaga
      121 ccgagcttta agctctgtag cgacgggcgt gcgtcccgtc cttcgttaag attgcaaaag
      181 ctcgacggct catagagccg tggctacagc ttcagcgttt ttggagcgag ttggctaatt
      241 gcgcggccaa acgtttcttg aactcggcgc ggaactcgat ttcatcgacg gctttagcga
      301 cgtccttcgc ggcggtcacg ggcaaattcg tcttcgcgta ttccctcagc tcagtcgccc
      361 gtgcgggatc agaaaaaaac gtgaacagac taggcgcgta actgttcgcg ccgagcgcat
      421 cgattttcgc caggagcgct ttcatgtttg ctttggcgaa gtcccacgcg aggtcagggc
      481 gctccgcata gcgtgcgact ttgccgacga gaaacacggc gcggctggtc ggcaactcgt
      541 ccgtcaacgc aatctgcaat gtcctagcga tgagcttcgg atcacgcacc ggcgtcagcg
      601 catcgtaata attttcctgt tcctcgctgc tcgtggtttt cagaccgagc tcatgcaagg
      661 tattccattc gttctcgccc ccgtaatggc cgaccaccca gaagaccggc caccgtaaat
      721 ctggcgtgag cgatgcaggc tcgctgagat atttctgaaa acgctctcga cacccagcga
      781 tcgttgtctc gtcaccgagc gcgccgagcg cctcgatcaa gcttccgcgc aaaatggcgt
      841 cgcgcggcgg ctcgtctttt ttaggtgacc aaccgagctg gtcgaagctc gggcgcaaaa
      901 tcgaatgagc gtagctttgg aacttctcac gaacaggatc gccgatgagc agccgattga
      961 tgtaatcaaa cgtgttgatg atctgctcgc gctctgccag ctcgatattt gccggaagct
     1021 ttccgacgag ctccagatac tggctgatcg gcgcgcggtc agcctgggcg aacgcccagg
     1081 cgtcacctaa caagttgacg cggtcgggca caccgagctt cggcagcgca ttgagcagca
     1141 acttccatga acgggcgtcg tactccacgc ggtaattgcc agcgccgtct gcgttcagtt
     1201 tcaatacgcc gtccgcggca atgttcggca gattgtcgat cttaccagtc atcaaccggc
     1261 tttgcgattt cgtatcgccg actacgaaat aggtgagggg aatcttccat tcgagcggcg
     1321 gcgcgtcggg gtaatgaatc gtgaaccgtt cctgactcag cgaaactttt tcgccgtccc
     1381 gttttacttt caggaccgga aagccgggtt gttccgtcca tcctgcagca atttcctcga
     1441 ccggctggcc ggacgcttcg cgcagtgcct tccacaaatc cgcggttgtc gaattggaat
     1501 atttgtgcgc ggcgatgtaa cgccggatgc catcacgaaa cgcgtcttcg ccgagaaaac
     1561 tctcgagcat gactaaaaac gcctggcctt tgcgataggt gatgtcgtca aacgcgctgt
     1621 tggcttcagc ttcattcgtg atcggctgct ggattgggtg agttgtcgat cttgcatcgc
     1681 cttccatctc cagctctttc gagattccgg tgcgccgtgt aggatcgcgc ggggtcactt
     1741 tttgcaacca gacttcccac tgtgggttga aacgcgcggt gcacttgctg cccatccagg
     1801 aggcgaagcc ttcgttcagc cagagattgt cccaccagcc catcgtcacg agatcgccga
     1861 accactggtg cgccacttcg tgcgcgatga cttcgtagat gatctggcgc gtcgactcag
     1921 acgatttctg cggatcgaac agcagcgcgg tttcgaagta agtgatgccg ccccagtttt
     1981 ccatcgcgcc gccgaacccg ccgggaatcg caatctggtc gagcttgggc aacggatacg
     2041 gcacgccgaa gtaatcgttg tagtaatcga gtatttgcgc ggtactctcg agtgcgtaac
     2101 ggccggttcc cgctttgcct ttggtcgtga caacgcggag ctcggtgcca gctttcgtcg
     2161 cgatcatgtc gagttcgccc gcgacgaaga cgttgaggta gctcgacatc gaaggcgtca
     2221 atgcgaaccg gacttctttg cgattgttgc cgatcttgcg ctcgagctcg accggcatgt
     2281 ttgacacagc cgtccagttt tccggaatca ccgcggttaa ttgaaaacgt gcgcggaagc
     2341 tcggttcatc ccagcaggga aaaaatcgcc gcgcatcggt cgcttcgaac tgtgtgccga
     2401 gcgccgtttt cttcgctccg gtaccttcct cctgataggg catgtagtag agcccgcggc
     2461 cttgctgatt gaccttgccc gaaaaactca gccggagctc atgtttgccg ggtgctagct
     2521 cggaggcgag cgcgatcctg agcagctcgt tctgggtgtc gatcttgaga gcgtttgccg
     2581 gaacagtttt gccgtcgagg gtcgcgctcc taatcgcgat atcggcggca ttgagcacaa
     2641 gctcgcgcac cggcgcgtgc acctcgagct ttatcgtttc ggaaccggta aaggtcagct
     2701 tctcgaggtt cggctcaacg cgaacggcgt attcgcttgg cacgacctgc ttcggcagtt
     2761 tgcctggtgt cgtagcgaaa tcgaatggtt tctctgccgc catttttccg acaaagagaa
     2821 agagaatggg cgcgaggaca agccagcgac gcatgcccca ctaaacagca cgcggcaaaa
     2881 tcgacaatct attgtacgac gaggatgagc aataactggc cattgccgac ctgcggtcct
     2941 ttaatgacga gcgggctgcg cttgctcagc ttcgtttccg tttcgagcag caggtcctct
     3001 tccttgtata gctttagatc gacaaggtag cccgcagatg tttcgccgcg cgcatcgacg
     3061 tgcaagccga agtatttgct cgacgccagc caatcttcct cgccagtttt cagcgtcttg
     3121 gtcgattggc caatgacctg gaattgatta tagccaaata attccttgag cgtttcctcg
     3181 atgcgcatca gcgccggcgg gatcggggcc ggttgggcga cgttttgggc aatgaccagc
     3241 ccactccaaa ccgtatgcgc ggcgtgcagc gacccggccg cgctcatcac cagcgcgaac
     3301 agagtgacga accacagccg acgagtttgc atcgcgcatt atttcgctgc gtaatcggcc
     3361 ggtaacgact gcagcccttc gacccacagc accgtgacct tgtcttcctt ggtttcgaaa
     3421 atcgagatgg tggcatttgg gctgacggcc gcatcgacgc gcgcgttgag gatttgggtg
     3481 agatactgcg attgctcggc cggattcttg tcgtgcatga caaacaccgc gcagaccagg
     3541 aacaacgcga gtgaaactgc accacttaac gccaggcgcg gcactgtcca ccatgtcgat
     3601 cttgtgcgat gccgatcttg cgcgcacgcg tcacgagcga gccgctcccg aatctgatga
     3661 ctaaaaaatt cctcattgtt cagcacctcg gtcccgagct cctgcttcaa caaaacacca
     3721 agcttgtgcg cggcgcgctt ctccgcttcg gcggcggcgc ggtcgggcaa cgacgcttcg
     3781 aactcctcca gctctttgcc ggtgagttcg ccatcgagcc aggcggtcca cttttcttca
     3841 aatgttttca tagacatccc tcaacaaatt ctgcaggcgc ttgcgcgcgt aaaagagccg
     3901 cgacatcacc gtgccgatcg agcagccgag cgtctcggca atttcatgat attgcatgcc
     3961 ttcgatctcc ttcatcagaa tgacggcgcg atgctccggc gaaagttgct tgatcgcgtt
     4021 gtcgattttt tcgcggatct cgccgcgctg catccgttca tgcggcagcg ggtcggcctt
     4081 cggcaaagtg cgcgaagcag gatcgacctc ctttaactga atggaatcat caaactccgc
     4141 gccggtgcct ttgacctgtt ttttgcgcag ccaatcgatc gtgacgttca tgacgatacg
     4201 gtaaatccaa gtgtaaaagg acgagtgccg gcggaagcgc ttgatcgact tccacgcttt
     4261 gacgaagctc tcctgcgcca ggtcccaggc gtcctgctcg ttatggacca tattataaat
     4321 catggcgaaa actcgggttc ggtagcggac caccagctcg tcgaaggcat cggtccgacc
     4381 agcctgacat cgtttgacca gttccagttc cgacacatcg gcttgagcca gagtcgcttc
     4441 cgtttgcatg tttggacgcg accggcgcct atttcattca agcgccgtta gcggctgccg
     4501 aagcggcgac gccattcgcc gcggtactcg cccatcagac tgatgatgcc atcgcggact
     4561 tcgaagaggc gtttctgcag gatggaatgc tggtccaccg tgatgtggcg gcctttgagg
     4621 agttgggcgg cggcctcgaa ggaggtggtg atcgctttca atgcgcgttt caaataggcg
     4681 atcgtcattc caatttcatc gagatcgtcg tcattcaacg cggcggcgag cttcgcgctc
     4741 gcgagcgcgg aatgcgttgc cagttttatc gcggccggtt cgttctggag cgccgcttcg
     4801 gcgtcgaaga gctgatcgat ccagacggtg agcgcgaagg ccgctcggta caagctgtgg
     4861 ttttcggaac tttccaaccg gattgcattg gcatcttcgt ccgcgaggtc ggcgccggag
     4921 caccagtctt ctttcgcgag cgcgtcgacc tcttcgctcc aatcgttatc ctcggcgaat
     4981 tgcgaccagc ccatctcgcg cgcgatgatt tcgtcccgct gtggatcgtc cagatatttt
     5041 tccagcagct ccatgtattt ctccgtcttt tggtcctgct gctggaggaa ccgttcccag
     5101 tcgtactcgt cccaaatttt gtccgactcg ggatcgtact cggccatacg tcgactaaac
     5161 gccagcttgc taactcgcgc aaaaagaatg tagcgcgagc gtctcgcttg ccctattgaa
     5221 gaagcaagcc ggaggcttgc tctacctttt ttgtctgcaa acgcaattgg ccgcacgccg
     5281 cttcgatgtc gtggcctttc tcgcggcgca gtgtcgcggc gactccggcg gagcgcagga
     5341 tcgacaagaa cttttcctgc cacgcgcgcg acggccgcga ccacgataat ccgacgacgg
     5401 tgttgtacgg aatgagattc actttcgccg agacacgccg cgcatgctgg acgagcaatc
     5461 gtgcctgctc gagcgcgtcg tttatgccgg cgatgaggat gtattcgaag gtgagccgtt
     5521 gtttcttacg cgcggcgtaa taatggcaag ccgccagcaa catgtcgatg ttgtaacgac
     5581 gattgattgg catgatttgg ttgcgcactt cgtccgttgc gccgtggagc gaaatggcaa
     5641 gacggatttg cagcggctcc tcggcgagtt tgcggatttg tggcacgagt ccactggttg
     5701 aaacggtgat gtggcgtgcg ccgatgccga ggccccacgg cgcgttcatg atgcgaatcg
     5761 cgcgcatgac gttctcgagg ttcgccagcg gctcgcccat ccccataaac acaatgttgt
     5821 cgatcttctc ggcggtggcg cgttcgaccg caatgatttg atcgacaatt tcgttggcgc
     5881 gaaggtttcg cgagaaacct tcgaggccgc ttgcgcaaaa tttgcagccg taagcgcagc
     5941 cgacctgtgt ggataagcag atcgtccggc gatccgcgtg cgtgccgtag agcgcgggcg
     6001 acgccggaat gagaacggac tcgatcagat tgccatcagc cagttgaaac aaaaacttgc
     6061 gtgttgtgtc gctcgaaccg agcacgcgca cgatctcgat cctggcgaag ccaaattccc
     6121 gctcgagccg ctcgcgcaga ttgcgcggca aatcggtcat ttcctggaac gactgagcgc
     6181 gttttttgta aagccaatcc gcgatttgcc cgacgcgata agcaggttcg ccagaagcgc
     6241 aaagccgttg ttgcagctcc tcgatgccta aagatttgag gtcgattttc gccggctctg
     6301 gcatgtacgc aatctagcgg gcgcgccgcc gaccagcaat gaacaggatg tattgtccag
     6361 acgttgcgct gcagttaact tcacgccatg aaattgttga tcgcgcgttt gtccgtgctt
     6421 gcgatcgcgt ggctggccag cgcttgtgcg tcgcagagcg aactgagtag cacgacaccg
     6481 catgcggcgg gagagggggc tgtgccgggc gaaaaagtat ccgatgagac gcagcttgcg
     6541 ccgggaacag gcgctaaccc caacgcaagc gtgcgctggt agcggttgtg cagaagtcgc
     6601 gcaatccgct cgcgctcggc tgggaagctg cacgggcgaa tgccgcgccg gcgctggtca
     6661 tccagctaat gatgctggcg ttgctcgttg cgtattacac cagtcctgca gcggcatcgg
     6721 cgctctcgcg cgtggccgaa ttcaagcgcg cccacggcat tgtatttgtc gtttccgcgt
     6781 cgatcgccgc aggggcgcta attcccgaaa tcttcctgat cctgtttttc cagcacgggc
     6841 ggccgagctc acgcaacctg cgcaatttgc tgttcacggt gccggtgtgg ggcttggacg
     6901 gatcactggt tgatcttttg tatcgcagcg aagcgcattg gttcggcgac gtggccacgc
     6961 tacccgtcgt cggcgcgaaa atctgtgtcg atcagttcgg ctacaatccg tttttcgcgg
     7021 cgccatttgg cgtgttgacc tacgaatgga aaaacagcgg aatctcgtgg cgaccggtgc
     7081 gcgatctttt cacctggccg cattaccgcg ataaaattgt gccgacgttg tgcgcgacat
     7141 gggcgatgtg gatcccactg atggcgatga tctattcgtt gccattggta ttgcagttcc
     7201 cactgttcgg gctggcgctc tctttctggg tgttgctgct gacgtatatg acaaatcgat
     7261 ttgccgcgaa aatcgaagcc gatgcgccgc tggcgttggc tgtcacagaa gcgacggagc
     7321 gcgccgagct tacttccggt gcccgttgag aaaaaactcc agcgcttcgt ggacgccggc
     7381 tccgtgctct ttgttggcga cgtaaccacc ggccgattgc accgccagct tcacttcaac
     7441 aattgcattt gccggacacg ccggcatgcc agcgatcttg ccgtcgagca tcgacacatc
     7501 gttgtgatga tcgccggcgg cgaaaatgtt ctctcgcggc acgtcgatta aacgggcgag
     7561 ttcagctagc gccgcgccct tgtgataatt ggcatggcaa aagcggagcc agagcgtgtt
     7621 gcgttggtag tggaagttcg gttgctgttc acgcgctctc tcgatgaatt tcacgatgcg
     7681 attcatctct tcgtcattcg aggcgactag tccttccaaa ccatcgctct ggtaaatcag
     7741 acgcgctttc gtcttctggt tcacaaaatc gacaatctcg gccagcaccg agtcggcgga
     7801 acggaaaagc tctgtgtggt cgcgggcgca gcgttcgttc caatcgccga atgcttccca
     7861 tttgtcgccg ttttggcccg gacggaagac gtgacgctcg cttgtgagaa tgaaatcggg
     7921 ccgcaacgga atagaaaaat cggtgagacc ggactcgagc agatccacgg accgaccggt
     7981 gttaatcgca aactgcacgc ctgacgtctg caattcccgg atcaagtcga tgcacgccgc
     8041 atcgagcacg ggttcgctca cgcgcgagac gagtgtgccg tcgaaatcga tgctgagaag
     8101 acggatgctc atggttcatc ttaccaattg cattcgatga ggagccggag caagggccac
     8161 gccgagcaaa tccgctactg cgggcgcgtg ccggcggccg cagcgattcg ccctggtgtc
     8221 ggagcatgcg gctgttttga cggctgcgtg ccgacgcctg acaatctgtc attgcgcaag
     8281 tcgagcgagc gttgcgcgag tcgcagccaa aaccgaacat cgcgctgagg aacggttgac
     8341 ggcgctgaac gcgaggggaa cataaccaag atcgacaatg aaatcggcct acgaactagc
     8401 gatggaacgg ctggaaaaaa accagccgag tgttgcctta actgccgaac agaagcaaca
     8461 gatcgcggag attgactcca gctacaaagc caaaattgcc gagaaagagt tgttgctgcg
     8521 cgacgaaatc cgcaaagcgc agacagccgg caatttcgag gaagcggaga agatcgagca
     8581 acagctcgcg agcgaggtca agcggttgcg cgacgattgc gacgcaaaga aagaaaaatt
     8641 gcgcgcgagt ttcgtgacca aatgattcta gggcaagcgc tccgcctgcc gccgatgcta
     8701 caaattgcgc ggcgaagccg ccatttgttc tgacatcgcc cgcggagcgg ccgattgacc
     8761 tttcccgcgc ggcggcaggt cggggtcaag gaaaccgagc atgtgggcgc gcttgatcac
     8821 acgcgtgcct tcctgttttt gcaagtcggc aatttgccgc cggaatgctt ctattttcgt
     8881 ccagtgcact tttggccgga aatgatgctc ggcgtgatag ccattgtaga agaagagcca
     8941 gttgtagagc ttgccgtaac tgcttacgcc ccaggctaca ggattatccg ggttgccgcc
     9001 gtagtggcga tagtacccgt tcaagaaact gaaacagtga ccgagatacc agaacggcag
     9061 aaagtagaag atcacgtaac gccaattgaa gcagaacagg atgagcagcg tggccgcgaa
     9121 tgccgccagc tcgatgttgc cccaggtaag gtcgccgtta ttgcgtttgc gtaattcttt
     9181 gcgaatcagg ccgacatcgt cgcgaaagaa actgagaaag acgtaactcc acggattctc
     9241 cggctcgcca tggtcgccgt gccggtaaat ggaaatccaa tcgacggtct cgcctttctc
     9301 atccggccga tcggcgttac ctttgtggtg ctgcatgtgg acgagatcgt aataggtctg
     9361 agagaaacaa caggcgatcg attgcgtgac gccgaaaaga cgattcagga tgttcgagcg
     9421 gaaaaacggg ttgtgaatga aattgtgacc aacgccgttg atgttggcgt tgatcatgag
     9481 cgagtaaacc aggccgagga taagcatcag ccaaagcggc gtccgcgggt aaagccagaa
     9541 cagcgcaaag aaatagccga gatgaaaaag acccgcgagc gcaggcacgg cgtcccagcg
     9601 cgtgtgcgcg aagagttgcg agttcgtggt gggccgctcc acgcgaatga cgtcagtgtt
     9661 catccaaaac tgaggacgcg aaattaatcg cggaagaaag tggcgctgac aagttttgaa
     9721 attgccattg tcgatcctgc gcgcctagtg tagcgggctt cagatgttcg cgctccagcc
     9781 ggagatgaag attttcagcg ggtcggctaa ccgcggtttg gccgagcgca tctgcagata
     9841 cattggcgtg ccgctcggtc aggcgacgct cagctcgttc ccagacggcg agacctacgt
     9901 caaaatcgaa gagaacgtcc gcggccgcga cgtgtttctc attcagccga cttcgccgcc
     9961 taccaatgag cacttaatgg aattgctcat catggtggac gcggcacggc gggcgagcgc
    10021 tgatcgtatc accgcggtta ttccattttt cggatatgcg cggcaggata ggaaagacaa
    10081 accgcgcgtg ccgatcaccg cgaagttaat agcaaattta ttgcacgctg ctggcgtgaa
    10141 ccgcgtgctg acgatggacc tgcacgcaca acaggtgcag gggttcttcg acattcccgt
    10201 cgatcatctt tactcactgc cagtgctaat cagatatctg cgaaagcggc tgaccggtaa
    10261 aaccgtcgtc gtgtcgccgg acatcggcgg tttgaaaatg gcatccgctt acgccgaagc
    10321 gctcggcgcc gggttggcga ttgtggctaa ggaacgcaag agtgcgaccg aaacggaagc
    10381 gctaaatttg atgggcgaag tcgaaggtca cgacgttttg ctggtggacg atctcaccga
    10441 aacggcgggg ac
//
LOCUS       DASTDG010000124         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1477164, whole genome shotgun sequence.
ACCESSION   DASTDG010000124 DASTDG010000000
VERSION     DASTDG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477164"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="uvrA"
                     /locus_tag="VFO30_03975"
     CDS             <1..1755
                     /gene="uvrA"
                     /locus_tag="VFO30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9418477.1"
                     /translation="QSEFTRNRIRSFMTRVPCKTCGGARLKPEILAVTIKSKWSRDAL
                     VPDEPARGRAGSSELNIHQFSELSVEAAARYIDKLELSAQQEAIVADVVHEIRSRLEF
                     LVEVGLGYLTLNRESGTLSSGEAQRIRLATQIGSGLAGVLYVLDEPSIGLHQRDNARL
                     LGTLRKLRDLGNSVIVVEHDEETIRAADHIVDLGPGAGPRGGEIVAEGTLENILRAQT
                     SLTADYLSGRLRIPVPKQRTRPRSIRSADVKNAEGWLVLEGAAENNLKRIEVVFPLGC
                     LICVTGVSGSGKSTLVDDILRRALFRRFYNSKDKPGAFRALRGVEQIDKAIVIDQSAI
                     GRTPRSNPVTYTGAFGPIRELFAQLPAARVRGYDAGRFSFNVKGGRCENCEGGGLIKI
                     EMHFLPDVYVQCEVCRGQRYNRETLEITYKGKNIADVLNLTVDEAARFFRNIPGVADR
                     LNALLDVGLGYLQLGQSGTTLSGGEAQRVKLAAELAKKATGRTVYILDEPTTGLHFAD
                     IEKLLQVLMKIRDAGNTLIVIEHNLEIVKCADWIIDLGPEGGERGGEVVAAGPPEAIV
                     DVAKSYTAQYLRPLLQKR"
     gene            1752..>3762
                     /locus_tag="VFO30_03980"
     CDS             1752..>3762
                     /locus_tag="VFO30_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418478.1"
                     /translation="MRTPALVLSALLSILLGRATRADISAQLAAASAPLVEGIPEVAV
                     VRLRALLTQNLKVEDWRTVAEKLAEALVATKQPQEALTVLSDPRLRNVTAAVFWRAQA
                     LASLQRWAEALSLYAQVARDQESPLGAEAIFGQAQALRALDRNDEALQALNNLFANKQ
                     WATQARLRAAELYVAKNDAANARRVLDELQPTNAAERKQRRLLRGRLELLLKKPDRAL
                     DAFEGLLKKPENVSHTILIAALSGIADAHLQLNTPESGDDVLENFIERHPQDVDLALV
                     FAKLDELYRAERKISRTELERWARDPSQPRRGLAQWYLARLDLRAGRRDRAMESFAAV
                     RRSETKFAELVPALLEYAQLTLEEGDLSSALGILNDARALQPNPTLRDRINLLAADVQ
                     YRMKRFESATAIFEQVAHSNSPWSKLALFNASIGWLRVGNYARFMADYANFEQQGGDA
                     DSGTRLRLEEGLMQAMKSDPKAAESLANFVREFPRDPHVSEAWVGLAELAFHSTPPRL
                     DEAKKDLAQAAAANLTPAATERADYLMIWIEDASSGNEARVIELANRFLQQHADSRYA
                     ADVRMKLAETYYRWHDLPNAQTQFELIAQQNPGSPLAEKALFFAAESAVASMGAHSLD
                     RALVLLDQVVRLNGELKWSARSEEAVVERKLGKPQDASLLYDEVLK"
BASE COUNT          803 a         1053 c         1211 g          695 t
ORIGIN      
        1 caaagtgaat tcacgcgcaa tcgcattcgc tcattcatga cgcgggtgcc gtgcaaaacc
       61 tgcggcggcg cgcggctgaa gccggagatc ctcgcggtga cgatcaaaag caaatggagc
      121 cgcgacgccc ttgtcccgga cgaaccggcg cgagggcgcg ccggctccag cgagctgaac
      181 attcatcaat tcagtgagct tagcgtcgag gcagcggcgc gttacatcga caaactcgag
      241 ttgagcgcgc agcaggaggc gatcgtggcg gacgtggtgc atgagatccg gtcgcgcttg
      301 gagtttctcg tcgaggtcgg tctgggttat ctcacgctca atcgcgagag cgggactttg
      361 agcagcggcg aagcgcaacg gatccggctg gcgacgcaga tcggctcggg tctcgccggc
      421 gtgctctacg ttctcgatga gccgagcatc ggcctacatc agcgcgataa cgcgcggcta
      481 ctcggcacgc tgagaaaact gcgcgacctc ggcaactcag tcattgtggt cgagcacgac
      541 gaggaaacga ttcgcgccgc cgatcacatt gtcgatcttg gacctggggc gggaccgcgc
      601 ggcggcgaga ttgtggccga ggggacactc gaaaatattt tgcgcgcgca aacttctctg
      661 acggccgatt atctctccgg acgtttgcgc attcctgttc cgaagcagcg gacaagaccg
      721 cggtcgattc gcagcgcgga cgtgaagaat gcggaaggct ggctggtgct cgaaggcgcg
      781 gcggaaaata accttaaaag gatcgaggtc gtatttccgc tcggttgctt gatctgcgtg
      841 acaggtgttt ccggcagcgg caaatccacg ttggtcgatg acatcctgcg ccgggcgctg
      901 tttcgccggt tctacaattc gaaagacaaa ccgggcgctt tccgcgcact gcgaggggtc
      961 gagcaaatcg acaaggcgat cgtgatcgac caaagcgcga tcggccgcac gccccggtca
     1021 aatccggtga cctacacggg cgcgttcggg ccgatccgcg aattgttcgc gcaattgccg
     1081 gcggcgcgtg tgcgcggcta cgacgccggg cggttcagtt tcaacgtgaa gggcggccgt
     1141 tgcgagaatt gcgaaggcgg cgggctgatc aagatcgaga tgcatttttt gccggacgtt
     1201 tatgtccaat gcgaagtgtg ccgcggccag cgctacaatc gcgagacgct cgagatcacc
     1261 tacaaaggca aaaacatcgc cgatgtgctc aatctcaccg tcgacgaggc ggcgcggttt
     1321 ttccgaaata ttcctggtgt ggctgataga ttgaacgcgc tgctggacgt cgggcttggt
     1381 tatctgcagc tcgggcagtc aggaactact ctctcgggag gcgaagcgca gcgcgtgaag
     1441 ctggcggcgg agctggcaaa aaaagcgact ggccggacag tctacatttt agacgagccc
     1501 acgacggggc tgcatttcgc cgatatcgag aagttgctgc aagtgctgat gaaaatccgc
     1561 gacgccggaa atacgcttat cgtcatcgaa cacaacctgg agattgttaa atgcgcggac
     1621 tggatcatcg atctcggtcc cgagggaggc gagcgcggcg gcgaagtggt cgcagccggt
     1681 ccgccggagg cgattgtcga cgtggcaaag agttacaccg cgcaatatct gcggccgctg
     1741 ttgcaaaagc gatgagaact ccggcactgg ttttgtctgc attgttgtcg atcttgttgg
     1801 ggcgcgccac gcgcgcggac atttctgctc aacttgctgc ggcgagcgca ccgctggtcg
     1861 aggggattcc cgaggtcgcg gtggttcggc tgcgtgcttt gctgacccag aatctgaagg
     1921 tggaagactg gcgcaccgtc gcggaaaaac tggccgaggc actggtggcc accaaacaac
     1981 cgcaggaggc actcacggtt ttaagcgatc cacggctgcg caacgtcacc gcagcagtat
     2041 tttggcgagc acaggcactc gccagcttgc aacgatgggc ggaagcattg tccctgtatg
     2101 cacaggtcgc gcgcgatcaa gaatcgccgc taggggctga ggcaatcttc gggcaagcgc
     2161 aagcactgcg tgccttggat cgcaacgatg aagcactcca agcgctgaac aacttattcg
     2221 ccaacaaaca atgggcgacg caggcgcggt tgcgcgcggc agaattgtac gtcgcgaaga
     2281 acgatgcggc caatgcgcgt cgagtactgg acgaactgca accgacaaat gccgcagagc
     2341 gaaaacagcg ccgccttctc cgcggccgac tcgagctgtt gctgaagaaa ccagatcgcg
     2401 cgctcgatgc attcgaaggg ctgctgaaaa aaccggaaaa cgtcagtcac acgattctca
     2461 tcgccgctct ctctggaatc gcggacgcac atctgcaatt gaatacgccg gaatcgggcg
     2521 acgacgtgct ggagaatttc atcgagcgcc atccgcagga tgtcgatctt gcgttggttt
     2581 ttgccaagct cgacgagctg tatcgggcgg aacggaagat ttcacggacg gaactggagc
     2641 gctgggcacg tgacccaagc caaccgcggc gcggtttggc gcaatggtat ctcgcgcgcc
     2701 tcgacctgcg cgccgggcgg cgggaccggg cgatggaatc attcgcggca gtccgccgca
     2761 gcgaaaccaa attcgccgag ctcgttcccg cgctgttgga gtacgcgcag ctcacgctgg
     2821 aggagggcga tctcagcagt gcattaggca ttctcaatga cgcgcgcgct ctgcaaccca
     2881 acccgacgct ccgcgaccgg atcaaccttc tcgcagcaga cgtgcagtac cggatgaagc
     2941 gtttcgaatc agcaacggca atcttcgagc aagtcgccca ttctaactcg ccgtggtcaa
     3001 agctggcgct cttcaatgcg tccattggct ggttgcgagt gggcaattac gcccggttca
     3061 tggccgacta cgcgaatttc gaacagcaag gaggtgacgc cgattcggga acgcggttgc
     3121 gactggagga aggtttgatg caggcgatga aaagcgatcc gaaagcggcc gaatcgctgg
     3181 cgaatttcgt gcgcgaattt ccgcgcgacc cgcatgtttc agaggcatgg gtcgggctgg
     3241 cggaactagc gtttcattct acgccgccgc ggcttgatga ggcgaaaaag gacctagccc
     3301 aggcggcggc ggcaaatctc actccagcag cgacggagcg cgcggattac ctgatgattt
     3361 ggatcgaaga cgcgagcagc ggcaacgagg cgagagtgat cgagctggca aaccgttttc
     3421 tccaacagca tgccgactcg cgctacgcgg ccgacgtgcg catgaagtta gccgagacct
     3481 actatcggtg gcacgatttg cccaacgcgc agacgcaatt cgaattgatc gcgcaacaaa
     3541 atccggggag tccgttggcg gaaaaggcct tatttttcgc ggctgaatca gccgttgcca
     3601 gcatgggcgc gcactcgctc gatcgcgccc ttgttctgct cgatcaagta gtgcggctga
     3661 atggggagct gaagtggagc gcgcgcagtg aagaggcggt ggtggagcgc aagctcggca
     3721 aaccgcagga tgcgtcattg ctttacgacg aagtgctgaa ag
//
LOCUS       DASTDG010000125         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_492088, whole genome shotgun sequence.
ACCESSION   DASTDG010000125 DASTDG010000000
VERSION     DASTDG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492088"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFO30_03985"
     CDS             <1..711
                     /locus_tag="VFO30_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418479.1"
                     /translation="IADADPANVVLLEIEPEKQKTRVDFAATQTLLGIRPVCVTDVKQ
                     RGRELFYRRDDNETRITRIYNRVIFDELSRRHDIDLAFRFQDELDVTWVGHPNWYFRV
                     SKHSLPFLKTEHTSPAFFADQFPDDEKIDDYVLKPLYSFAGLGVDMEPSCEKLAGLTN
                     PHDWILQKKVEYAPFVPTVDGHRSKAEIRMMFVWPEEDREPTLVNNLVRMSQGKMMGV
                     DFNKDKTWVGSSIALHES"
     gene            complement(763..1203)
                     /locus_tag="VFO30_03990"
     CDS             complement(763..1203)
                     /locus_tag="VFO30_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418480.1"
                     /translation="MAPPVIDLQSRERLGRVGGMTKYFSFRMFMGAVVMSALAACATH
                     PMLLRPPLIGTWTNSLGTVWMIKADGTFDVDLNHHGQRDAWGRWSVTGDTVTLVRVGG
                     IRPKGCDGEGVYHFMRSGDTLRFRLVSDACKLRRKNVLLAWHRR"
     gene            1240..2283
                     /locus_tag="VFO30_03995"
     CDS             1240..2283
                     /locus_tag="VFO30_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9418481.1"
                     /translation="MSAISEDQIKEALKSVKYPGFTRDIVSFGLIRSVQIGDGEVKVQ
                     LALATNDPNVPATIKTDAERTLRSLPGVQSAKVLIDIHAPPGGAAAGAVAAHIPGVKH
                     VIAVASGKGGVGKSTVAANLAVALQQTGALVAICDCDIYGPSISLMFGTRERPMATDE
                     NKIVPVEQYNLKLMSMGLLLDDTSPAILRGPMVTRYTQQFLRQVDWGELDYLVLDLPP
                     GTGDIQLTIVQTVALSGAIIVTTPQEVALIDARKAATMFEKVNVPVLGLVENMSYFVS
                     PSDGKRYDIFGSGGGEREAKRLRVPLLGQIPIDIPTREAGDRGVPVTAEDRQSPVAAE
                     FARIARRIRETLL"
     gene            2392..2616
                     /locus_tag="VFO30_04000"
     CDS             2392..2616
                     /locus_tag="VFO30_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418482.1"
                     /translation="MPAHEDRAEFVKNSVLYKEFLAEREEILKHKWIESEKAGKDIGF
                     ERALLDWIVKHRSNWREKRIKEARTAKATS"
     gene            2664..3224
                     /gene="pyrR"
                     /locus_tag="VFO30_04005"
     CDS             2664..3224
                     /gene="pyrR"
                     /locus_tag="VFO30_04005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9418483.1"
                     /translation="MNRKTQDPSAAVQILDADAIQRAVRRIAHEIVENNRELKSIVLA
                     GIPSRGVELASRIAGAIRSIAKIDIDSGVIDVAMHRDDIGRRADLPVVRASKLPTPLE
                     GRTVIIVDDVLYTGRTIRAAMDAMNSFGRPARIQLAVLIDRGHRELPIRADYVGKNLP
                     TAPSDKIRVQLEQTDGELNAVWLVKQ"
     gene            3400..>3730
                     /locus_tag="VFO30_04010"
     CDS             3400..>3730
                     /locus_tag="VFO30_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HET9418484.1"
                     /translation="MTTHWTRKDLLGLRELSADEINVVLESADAFKGVGTREIKKVPA
                     LRGKTLVNLFVEPSTRTRTSFEFAAYRLSADVINISASTSSLTKGETLKDTARNLEAL
                     HADILVLR"
BASE COUNT          822 a         1081 c         1100 g          727 t
ORIGIN      
        1 atcgccgacg ccgatccggc aaacgtagtt ctgcttgaga tcgagccgga aaaacaaaag
       61 acgcgcgtcg attttgccgc gacgcagacg ttgctcggga ttaggcccgt gtgtgtgaca
      121 gatgtaaagc aacgcggtcg cgaattattc tatcggcgcg acgacaacga gacacgaatc
      181 acgcgcatct acaatcgcgt cattttcgac gaactatcac ggcggcacga catcgatctg
      241 gcgtttcggt ttcaggatga gctcgatgtc acctgggtcg gccacccgaa ttggtatttc
      301 cgcgtcagca aacattcctt gccgttcttg aagaccgagc atacgtcgcc agcgtttttc
      361 gccgaccaat ttccggatga cgaaaagatc gacgattacg tgttgaaacc gctctattcc
      421 ttcgctggtc tcggcgtcga catggagccg agctgcgaaa aacttgctgg cctaacaaat
      481 ccgcacgatt ggattctgca aaagaaagtg gagtacgcgc cgttcgtgcc gaccgtcgac
      541 ggccaccggt ccaaagccga gatccggatg atgtttgtct ggccggaaga agaccgcgag
      601 ccaacgctgg tgaacaatct cgtccgaatg agtcagggga aaatgatggg cgtggacttc
      661 aacaaagaca agacttgggt cggctcaagc atcgcgctgc acgagagctg acccgtccgc
      721 cccggcttta ggtaacgacg acactcgtcg cgcggcgcga acttagcggc ggtgccaggc
      781 aaggagcacg tttttgcggc gcaatttgca cgcatcgctg acgagcctga accggagcgt
      841 gtccccgctg cgcataaaat gataaacgcc ttccccgtcg cagccttttg gtctgatgcc
      901 gccgacccgc acgagcgtga cggtgtcgcc ggtaacgctc cacctgcccc aggcatcgcg
      961 ctggccgtgg tgattaagat cgacatcgaa ggttccgtcg gccttgatca tccagacggt
     1021 gccgagggaa ttcgtccatg tgccgatgag cggcggtctt agcaacatcg gatgggtcgc
     1081 gcaggcagca agggccgaca tcacaaccgc gcccatgaac attcggaatg agaaatattt
     1141 tgtcatgccg ccgactctgc ctaaccgctc tcgcgactgc aagtcgatca ccggcggcgc
     1201 cataacttta ctccacccct gcgcttcgta ctttaaggtg tgtcggccat ctccgaggac
     1261 caaatcaaag aagcgctaaa gtctgtcaaa tatccgggct tcacgcgcga catcgtatcg
     1321 ttcggcctga tccgctcggt ccagatcggg gacggcgaag tcaaggtgca gctcgcgctt
     1381 gccacgaatg atccaaacgt gccggcaacg attaaaactg atgccgagcg aaccttacgc
     1441 agtctgcccg gtgtgcagag cgcgaaagtg ttgatcgaca tccatgctcc gcctggtggc
     1501 gccgccgccg gcgctgtggc agcgcacatt cccggtgtca agcacgttat cgctgtcgcg
     1561 agtgggaagg gtggtgtcgg caagtcgacg gtcgcggcaa atctcgcggt cgcactccag
     1621 caaacgggtg cgctggttgc gatttgtgac tgcgacatct acggcccgag tatttcgctc
     1681 atgtttggca cgcgcgagcg gccgatggcc acagacgaga acaagattgt cccggtcgag
     1741 cagtacaacc tcaagctcat gtcgatggga ctcctcctcg atgacacgtc gccagcgatt
     1801 ttgcgcggac ccatggtgac ccgttataca caacaatttc tccggcaggt cgactggggt
     1861 gagctcgatt atctggtgct cgatctgcca cccggcaccg gggatatcca gctcaccatc
     1921 gtgcagacgg tcgccctttc cggcgccatt atcgtcacga ccccgcagga ggtggcgcta
     1981 atcgatgcgc gcaaggccgc gaccatgttc gaaaaagtca acgtgcctgt gcttgggctg
     2041 gtcgagaaca tgagctactt cgtttcgcca tccgatggta agcgctacga catcttcggc
     2101 agcggcgggg gcgagcggga agcgaaacgg ctgcgcgttc cattgttagg ccaaatcccg
     2161 atcgatatcc caacccgcga agccggtgat cgcggcgtac cggtgacagc ggaagatcga
     2221 caatcaccgg tggcggcgga gttcgcccgg atcgcgcgga gaatccgcga aacactgctg
     2281 taacagaacg gtcacttgat taaataggta tggctgatgc tacgtcagac tttgatcgga
     2341 aacataatca gttattgacc gattctgagt tttatggaaa acaagccggg tatgcctgca
     2401 catgaggatc gggccgagtt cgtcaagaac tccgtcctct ataaggaatt cctggccgag
     2461 cgagaagaga ttctcaagca caagtggatc gagagcgaga aagccggcaa agacatcggc
     2521 ttcgaacgcg cgctgctcga ctggatcgtc aaacatcgct cgaactggcg tgagaaacga
     2581 ataaaggagg ctcgcaccgc gaaggcgacc tcctgaaatt cgctctcctt taacgcgatc
     2641 ccgcgaggtc gccaaggaat gcgatgaacc ggaagacaca ggatccttct gccgccgtcc
     2701 agatcctgga cgcggatgcg atccaacgcg cggtgcggcg catcgcgcac gagatcgttg
     2761 agaacaatcg cgagctgaag tcgatcgtgc tcgcggggat cccttcccgc ggggtcgagc
     2821 ttgcatcgcg aatcgccggc gcaatacgct cgatcgcgaa gatcgacatt gattccggag
     2881 tcatcgatgt cgcgatgcac cgcgacgata tcgggaggcg cgccgactta ccggttgtgc
     2941 gtgcgtcgaa gctgccgacc ccgctggaag ggcgcaccgt catcatagtc gatgacgtgc
     3001 tttacaccgg tcgcacaatc cgcgcggcga tggacgcgat gaattccttc ggacggccag
     3061 cgcggattca actcgcggtc cttattgatc gcggccatcg cgaactgccg attcgcgccg
     3121 attacgtcgg caagaatttg ccaacggcgc cgagtgacaa aatccgcgtt caactcgagc
     3181 aaaccgatgg cgaactgaac gctgtgtggt tggtgaagca atgagtgtga acgcgcggtt
     3241 tctgggtagc gcacgcgtct cgcgtgctgg cgagcgcgtc ctcgcgctcg cgaacttttc
     3301 ttttgatttc gctgcgaacg agattggcac acgcaagaaa agattgtttc ggcgagacgc
     3361 cgaaaccagc aagcgagacg cgtgccctac ccagacaaca tgaccacgca ttggacgcgc
     3421 aaggatctcc tcggtttgcg cgagctttcg gccgacgaga tcaacgtcgt gctggaaagc
     3481 gccgacgcct tcaagggagt cggtacccgc gagatcaaaa aggtcccagc gctgcgcggc
     3541 aaaacgctgg tgaacctttt tgtggagccg agcacgcgca cccgcacctc gttcgagttc
     3601 gctgcctatc gattgagcgc tgacgtcatt aacatttcag cgagcacgtc gagcctgacg
     3661 aaaggcgaga cgttgaagga cacggcgcgc aatctcgaag ctttgcacgc cgatatcctg
     3721 gtgctgcgtc
//
LOCUS       DASTDG010000126         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1107195, whole genome shotgun sequence.
ACCESSION   DASTDG010000126 DASTDG010000000
VERSION     DASTDG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107195"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VFO30_04015"
     CDS             complement(<1..1306)
                     /locus_tag="VFO30_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET9418485.1"
                     /translation="MRTLLHHTLLTLLACLGLPLLTQASDPEGILPNGNTAEGFAVLA
                     HLTSGGANSGLGFQSLFNDTAGNGNSGVGFQSLFNDKGGSFNTAMGYRSLFNDTSGIM
                     NAAFGFEPLFTNTTGSRNTAIGNGALFHNNAGTNTAVGWASLFSNTTGSACTAVGYRA
                     LVSNTVEIGNTAVGSEAAAASTIGEEISAFGFRALFSNTAGHNSAFGYKALISNTTGD
                     GNTGVGAEALELNISGGNNTALGFNALINSGSGNANIAIGANAGIAVTTASDVIAIGL
                     PGANVSGTCFIDSIRGVTTQNANAVNVVIDSAGQLGTVSSSRRYKDNIQRMEKASEAL
                     LALRPVTFNYKSDKTATSQFGLIAEDVAEVNPDLVVRDGKGEIYSVRYDAVNAMLLNE
                     FLKEHRKVQEQQATIAELKKDVGALVTRIKEQDRKIQKVSDQV"
     gene            complement(1525..2562)
                     /locus_tag="VFO30_04020"
     CDS             complement(1525..2562)
                     /locus_tag="VFO30_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418486.1"
                     /translation="MRQDPDDFRLVVLSPGGRDPEQHFPDGAGGLEQAHPPVNFHGFA
                     ACTRGYFHRDVQRAAAEGLPVLLLLRGDFHASERALAELQQDGRKVVVSLKETGLDQI
                     AQQLANPRMLSRFLRLVRQADGCIGTTPEAAEIYRRVRGGEDDGRVAFIPTPYPLEDR
                     QWDFSVIPDKQTGIFVGTREWDVPSRNHFAAVLLARQLCETTDDPVTIVNLEGRKGRH
                     LLDELKFPEGKLRLIEKRNAYPDFLREVARHKIVLQLDRSRVPGQVAGDALLCRAACV
                     GGDGAIERIAFPRTCGHGRNAAEIESIAQQLLGNANSRGALVMESQWRALERVSFSAV
                     RTQLANFFVRL"
     gene            complement(2580..5180)
                     /locus_tag="VFO30_04025"
     CDS             complement(2580..5180)
                     /locus_tag="VFO30_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET9418487.1"
                     /translation="MIPTSRYEYRRPWYATPQVYIPVVLVALFVIAVAIYAVIVTAQL
                     QARAATFDLSKLEQMESASVILDRNGKIFGQIYVENRETIPYEQLPRDLINAVVAVED
                     AKFYRHHGYDLFGIVRATLKNVVSGRVRQGASTITQQLARNSFALKEKTFRRKLLEIF
                     LARRIEDQFGKQKIMELYLNRIYFGAGLYGAEAAARGYFGKHARDMSLAECATLAGLV
                     KSPNRLSPWSDRAASRDARNYALGRMRDLGLIDNERFTRAQAEDLVIGNRQNAQGQNY
                     AVDYIRQQVINAVGWDRAMNEGFRIRTTIDGDLQKVAEDSLRKSLEDTERHPDYHHQT
                     YAEYAANFRKAKSSSALANQPAPEYLQGAVIGLDNETGGILVLVGGRDFEHNQYDRAL
                     QAKRPAGTAMLPFVYAAAFENGSYPGSVVDDSPLDNRAVMIGGTTGILGEWGPESADN
                     RYEGAITARQALANSKNGATVRIGMDAGLDNVLQLCRNAGVRSALRPYPATFLGSSEI
                     TLAELALAYTIFPNGGWRPNTPHILERIEEKDGSVVWDANQERGRQNVIKPESTYEVH
                     SCLVDALESGTGKAARTQFGLKKFPAAGKTGTAYDFTDALFAGYDSNFTCAVWAGFDK
                     PQKIYRGAFGRELALPVWVDVMNAAAEHYPPREIKRPPTLREVEICSRSGLLATDRCY
                     DAIKTATGDEVHRRTTYTEIASPAQIPTEPCNVHGDPNARIAREFASNESDLPRAELA
                     VDLSNIRPIIVKGPTLLVDKDPYNSIRATTKPQPQSEEQTAEQAKIDNAPPLPHAVAV
                     KPPKAEPVETPPKAEPVETPSVTAPIRKALPVKPEEEETPGEIRKAQPVGPLDEEQEQ
                     PLLKAETPPP"
     gene            5341..6447
                     /locus_tag="VFO30_04030"
     CDS             5341..6447
                     /locus_tag="VFO30_04030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9418488.1"
                     /translation="MNTELVQFIHETICARGPQPFAWFMQQALYHPQHGYYASGRAAI
                     GRHGDYFTNVSVSPLFGQLLTIQFAEIWEHLGNINKFTIVEQGAHEGAFARDVLRCAR
                     ECFPKFFAALRYRIIEPFPALQDRQSQTLAAFRDKVEWAKTLTPFVGVHFSNELLDAL
                     PINLRGKRVGLAGDEFVFVQCADELETKPNQAALDWVDNVTANLERGVVIAVDYGFTR
                     SVFHESLQVRAQHRRLASPFEQIGRADITMHVNWTDIAERLKAKGLHIAGFTDQHHFL
                     TGILSADAEILHASCQLADGKKSVNDLLDLTALSRQVRRERDEKISRALQTLLHPEML
                     GRAFQVLALSKDVDVVGRLCGFKFARDARVELEL"
     gene            complement(6444..7880)
                     /locus_tag="VFO30_04035"
     CDS             complement(6444..7880)
                     /locus_tag="VFO30_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9418489.1"
                     /translation="MAEQWDAVVIGSGPNGLAAAITLAQAGCSVLVLEANATIGGGAR
                     SAELTLPGFLHDLCSAVHPLAAGSPFFKSLPLERHGLHWIQPEIPLAHPLDDGSAACL
                     HRDVDLTIDSLGRDGARYGRLMKPLVRNWEKLAPEFLQPMLHLPRHPFALARFGIPAM
                     CPTTLLVKTLFKSEPARALFGGIAAHSFLPLEAPVSAAFGLVLGMAGHAVGWPIPRGG
                     SQSIANALAAYLRELGGRIDVNCRVGNLAQLPPSCAVLFDTSVWNFARLVGSRLPDRY
                     GRRLENFRHAPGVFKIDYALSDPVPWKAAVCKRAGTVHLAGTFAEITWSERQVARGEI
                     PERPFVLVSQQSLFDSTRAPEGQHTLWAYCHVPFDCNIDMSQRIEAQIERFAPGFRDC
                     VMARHKMSAVDLAEKNLNLIGGDINGGAANFWQLIARPVLSATPYRTPLRGIYLCSSS
                     TPPGGGVHGMCGYHAARAALGDLFGNSH"
     gene            7960..8499
                     /locus_tag="VFO30_04040"
     CDS             7960..8499
                     /locus_tag="VFO30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HET9418490.1"
                     /translation="MSAPRPRNRRRNADFSVEKYGERTTEKEECEEQLLAEYAVLLAF
                     YLTTVAVLTGAAVERGRLPRKFGLLDLALLGVATHKLSRIVAKDRITGIVRAPFVKYI
                     RSSGAGEVEEEPRGRGIQRGIGRLISCPYCMAPWCGTALGFGFVFAPRVTRFFAGIFA
                     SVAVSDFLQRAYSAAKEKW"
     gene            complement(8596..>9072)
                     /locus_tag="VFO30_04045"
     CDS             complement(8596..>9072)
                     /locus_tag="VFO30_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9418491.1"
                     /translation="TGVPLALTGGTLALWLRGLPFSISAGVGFIALSGVAVLNGVVMV
                     SFIARLRESGHSLEDAIRVGSLTRLRPVLMTALVASLGFLPMALAHGRGAEVQRPLAT
                     VVIGGIISSTALTLIVLPVLYRIFHRKELGSYRGQLPGRAIGEEQEEDLPAVSTIS"
BASE COUNT         1835 a         2704 c         2628 g         1905 t
ORIGIN      
        1 cgacttggtc actgaccttc tgaatcttcc tatcctgctc tttaatccgc gtaacgagcg
       61 cgccgacatc cttctttagc tccgcaatgg ttgcttgctg ttcctgcact ttgcggtgct
      121 ctttcaggaa ctcgtttagt aacatcgcgt tcaccgcgtc atagcggacg ctgtagatct
      181 cgcccttacc gtcgcgtacc acgaggtccg gattaacttc ggcaacgtcc tcagcaatca
      241 gcccgaactg tgatgtggcg gttttgtcgc tcttgtaatt gaaagtaacc ggccgcagcg
      301 ccaggagcgc ctcgctcgct ttctccatac gttggatgtt gtctttgtac cggcgggaag
      361 agctgacagt gccgagttgt ccagccgagt cgatgacgac gttaaccgcg ttcgcatttt
      421 gcgtcgttac tccgcgaatg ctgtcgatga aacaggtgcc actcacgttc gcgccgggga
      481 gtccaatggc gatgacgtcg ctggccgtcg taacagctat gcccgcgttc gccccgatag
      541 cgatattcgc gttgccgctg ccgctgttga taagagcatt gaacccgaga gctgtgttat
      601 taccgccgct gatattgagc tcgagtgcct cggcgccgac gccagtgtta ccgtcgccgg
      661 ttgtgttcga tataagcgcc ttatatccga acgcgctgtt gtggccggct gtgttgctaa
      721 acagagctcg aaagccaaag gcacttatct cctcgccaat cgtgctcgca gctgccgcct
      781 cacttccgac ggcggtattg ccgatctcaa cggtgttgct gacaagcgcg cgataaccca
      841 ccgctgtgca cgcgctgccg gtcgtattgc tgaacaaaga cgcccacccg acggcggtat
      901 tagtcccagc gttgttgtgg aataacgcac cgttaccgat tgctgtgttg cggctgccgg
      961 tggtgttggt gaacagtggc tcaaatccaa aggccgcgtt cattatgccg ctggtgtcat
     1021 taaacagaga gcggtaaccc atggccgtgt taaagctgcc gcccttatcg ttaaacaggc
     1081 tttggaatcc aacgccgctg ttcccattgc cggcggtatc gttaaacagg ctttggaacc
     1141 ctaagccgct gttcgcaccg ccgctggtca gatgagcaag caccgcgaaa ccctcggccg
     1201 tgttgccatt cggcagaatg ccctcggggt cgctagcttg cgtaagaagc ggcaggccaa
     1261 gacaggccag cagcgttaaa agagtgtgat gaagtaaagt gcgcattcga ttgatccctt
     1321 cgtcccgctt gttcaacatt gttgtagcgc gcaatcagag aaaagaggcc gcgggcaaac
     1381 tccccagctc cgaccgtacg cccattgatt tgcgtgcaac ccttacgaaa aagtccggct
     1441 cgtcaacatt aaaccgcagg actcagttcg gatccggcag agaaggttgt tcgatcgcca
     1501 agccgcgttc gcggagcgaa gcagctacag tcgcacgaag aaattcgcga gctgcgtgcg
     1561 cacggcggaa aacgaaacgc gttcgagagc gcgccactgc gattccatca caagcgcacc
     1621 gcgcgagttg gcattgccga gcaattgctg cgcgatcgat tcgatttccg cggcattgcg
     1681 gccatgaccg catgtgcgcg ggaatgcgat gcgttcaatt gcaccgtcac cgccgacgca
     1741 tgcagcgcga caaagcaatg cgtcacctgc gacttgtccc ggaacgcggc tgcggtccag
     1801 ctgcaaaacg attttgtgcc gcgcaacttc gcgcagaaaa tcagggtagg cgttgcgctt
     1861 ctcgatcaaa cgcagttttc cctcgggaaa tttcaattcg tcgagcaagt gacggccttt
     1921 gcgtccttcg aggttaacga tggtgaccgg atcgtctgtg gtttcgcaaa gttgtcgcgc
     1981 caggagcacg gccgcgaaat gattacgcga aggaacatcc cactcgcggg tcccgacaaa
     2041 gatgccggtc tgtttgtccg gtatcaccga gaaatcccac tgtcgatctt ccagaggata
     2101 tggcgtcgga atgaatgcga ctctgccgtc atcttctccg ccacggacgc gtcgataaat
     2161 ttcggcggct tccggcgtcg tcccgatgca accgtcagcc tgcctgacga gtcgcaaaaa
     2221 gcgtgacagc atccgcggat tcgccagctg ttgggcaatt tgatccagac cggtctcctt
     2281 gagcgacacg acaaccttgc gtccatcctg ctgcagctcg gctaatgcac gctcggaagc
     2341 atgaaaatcg ccgcgcagca ataagagcac cggcaggccc tcggcggccg cgcgttgcac
     2401 gtcgcgatga aaatagccgc gtgtgcatgc ggcgaagcca tggaagttta ccggcgggtg
     2461 cgcctgctca agtccacctg cgccgtcagg gaaatgctgc tcggggtcgc gcccgccggg
     2521 actgagaacg acgaggcgaa agtcatctgg gtcttgcctc atgttaatgc cgcgtcgggt
     2581 cagggcggcg gtgtttcggc tttcaacagc ggctgttcct gctcttcatc caaggggccg
     2641 accggctggg ctttgcgaat ctcgcccggc gtttcctctt cctcgggttt aaccggaagc
     2701 gctttccgaa tcggcgcggt caccgacggt gtctcgactg gctcggcttt aggcggggtt
     2761 tccactggtt cagccttggg cggttttacc gctacggcgt gcggcagggg cggcgcattg
     2821 tcgatcttag cctgctcggc cgtttgttct tccgattgcg gctgcggctt ggtcgtggcc
     2881 ctgatggaat tgtacgggtc tttgtcgact aacaaggtgg gccctttgac aatgatgggc
     2941 ctgatgttgc taagatcgac agccaactcg gcacgcggta aatcgctctc atttgacgca
     3001 aactcccgcg caatgcgggc gtttgggtcc ccgtgaacat tgcacggttc ggtcggtatc
     3061 tgcgcgggcg aggcgatttc cgtgtaggta gtgcgcctgt gcacctcgtc accggtcgcg
     3121 gttttgatcg cgtcgtaaca ccgatccgtg gcgagcaggc ccgaacgtga acaaatttct
     3181 acctcgcgca aggttggggg gcgtttgatc tcgcgcggcg gataatgttc cgccgctgca
     3241 ttcatgacgt cgacccatac gggcagcgcg agctcgcgcc cgaatgcgcc gcggtaaatt
     3301 ttttgcggct tatcaaagcc ggcccagacc gcgcacgtga aattgctgtc gtaaccggcg
     3361 aaaagcgcgt cggtaaaatc gtaggccgtg ccggtttttc ctgcggccgg aaatttcttc
     3421 aggccgaatt gcgtgcgcgc cgctttgccg gttccggatt ccagcgcatc gacgaggcag
     3481 gagtgaactt catacgtact ctctggcttg atcacatttt gtcgcccgcg ctcctggttc
     3541 gcgtcccaaa caacgctgcc gtccttctcc tcgatgcgtt cgaggatatg cggagtgttt
     3601 gggcgccagc cgccgtttgg aaaaatcgtg taggcgagcg cgagctcagc gagcgtgatt
     3661 tcgctgctac caagaaacgt cgcgggatac ggccgcaacg ccgaacgaac gccggcgtta
     3721 cgacacagtt gcagcacgtt gtcgaggccc gcgtccatgc cgattcggac ggttgcgccg
     3781 ttcttcgaat tggccagcgc ctgccgcgcc gttattgcgc cttcgtaacg attgtccgcg
     3841 ctttccggtc cccattcgcc aagaattccg gttgtgccgc cgatcatcac ggcgcgattg
     3901 tccagcggcg aatcgtccac caccgatccc ggatacgagc cattttcgaa cgcggcggca
     3961 tacacgaagg gcagcatcgc tgtgccggcc gggcgcttcg cctgcagcgc gcgatcgtac
     4021 tggttgtgct cgaaatcgcg tccgccaacg agcacgagaa tgccgccggt ttcgttatcc
     4081 agaccgatga cggcgccttg caaatattcc ggcgccggct ggttcgcgag cgcgctgctc
     4141 gacttcgctt tccggaaatt ggccgcgtac tcggcgtaag tctgatgatg gtaatcggga
     4201 tggcgttccg tatcttccaa gcttttccgc aacgaatcct ccgccacctt ttgcaggtcg
     4261 ccgtcgattg tcgtgcgaat ccgaaaaccc tcgttcatcg cccggtccca gccgacggcg
     4321 ttgatcacct gctgccgaat gtaatcgacc gcgtaatttt gcccctgtgc attttgccgg
     4381 ttaccaatca cgagatcttc ggcctgcgcg cgcgtgaacc gttcattgtc gatcaaaccc
     4441 aggtcgcgca tccggccgag cgcataattg cgcgcgtcgc gagacgcggc gcggtcactc
     4501 cacggcgaca accgattcgg actcttcacc aacccggcga gcgtcgcgca ttcggccagg
     4561 gacatgtcac gagcgtgctt tccgaaatag ccgcgcgccg ccgcttccgc gccgtaaagt
     4621 cccgccccga aataaatccg gttcagatac agttccatga tcttttgttt gccgaactga
     4681 tcttcaattc ggcgggccag aaaaatctcg agcagtttgc gccggaacgt tttttccttg
     4741 agcgcaaaac tgttgcgcgc cagttgctga gtgatcgtgc tcgcgccctg gcgcacgcga
     4801 ccggacacga cgttcttcag agttgcgcgc acgatgccga acaggtcata gccgtggtgc
     4861 cggtaaaact tcgcgtcctc gaccgcaacc acggcgttga tcaggtcacg cggcaactgc
     4921 tcgtagggaa tggtctcgcg gttctcgacg tagatttgtc cgaaaatttt gccgttgcga
     4981 tccagaatca cgctggccga ttccatctgt tcgagcttgg agaggtcgaa cgtcgcggcg
     5041 cgcgcttgca attgcgcagt cacgatcacc gcgtaaatcg ccaccgcgat gacaaacaag
     5101 gcgaccagga ccaccggaat gtaaacttgt ggtgtcgcgt accacggccg gcgatattca
     5161 tatcgcgacg tcggaatcaa aaatggcggc gaagtcctca atcttttccc cgggcgatca
     5221 gtcgcgcaat ctagaagaaa acgccgaacg ctcaacgctc aacgccgaat ctccaatgcc
     5281 gaggatacct ctgcaattct cccattggac gttggatgtt cggcgttcga cgttttctgc
     5341 ttgaacacag aattggtcca attcatccac gagacgatct gtgcacgagg tccgcagccc
     5401 tttgcctggt tcatgcaaca ggcgctttat catccgcagc acggttatta cgccagcggc
     5461 cgcgccgcga ttggacgcca cggtgattac ttcactaacg tcagcgtcag cccgcttttc
     5521 ggacaactgc ttaccattca gttcgccgag atttgggagc accttggcaa catcaacaag
     5581 ttcaccattg tcgaacaagg cgcgcatgag ggcgccttcg cgcgcgatgt gctgaggtgc
     5641 gcgcgcgaat gcttcccgaa gtttttcgcc gcgcttcgat accggattat tgagccattt
     5701 ccggccttgc aagatcgaca atcccaaacg ctggcagctt ttcgcgacaa agtggagtgg
     5761 gcgaaaacgc ttactccctt tgttggcgtt catttttcga atgagctgct cgacgcgctg
     5821 ccaattaatc tccgcggaaa gcgggtcggc ctcgccggcg acgaattcgt ttttgtccaa
     5881 tgcgccgacg aactagaaac aaagccgaat caagccgctc tcgattgggt cgataatgtc
     5941 accgcgaact tggagcgcgg cgttgtcatc gcggtcgact acggcttcac ccgcagcgtt
     6001 ttccacgaat cattgcaagt gcgcgcgcaa caccgacgtc tggcgtcacc attcgagcaa
     6061 atcggccgtg ccgacatcac aatgcacgtc aactggactg atatcgccga acgcctcaaa
     6121 gccaagggtc tgcacattgc cggcttcacc gatcaacatc atttcctgac cggcattctc
     6181 tcggccgacg cggagatcct ccacgcttcc tgccagctag ccgacggcaa aaagtcggtc
     6241 aacgacttgt tagacttgac ggctttaagc cgtcaagttc ggcgcgagcg cgatgaaaaa
     6301 atatcgcgcg cattacaaac cttgctccat cccgaaatgc tcggtcgtgc gtttcaggtc
     6361 ctcgcattga gcaaagatgt cgatgttgtg ggcagacttt gcggcttcaa gttcgcgcgc
     6421 gatgcccgcg tcgagctcga gctctaatgg gaattcccga acagatcccc taatgccgcg
     6481 cgagccgcgt gatagccgca catcccgtga acgccgccac caggcggagt tgaggacgaa
     6541 cataaataga tgccgcgcag aggcgtgcgg tacggagtcg cactgagaac aggtcgcgcg
     6601 atcaactgcc aaaagtttgc cgccccgcca ttaatgtcgc cgccgatcaa gttgagattc
     6661 ttttctgcca gatctaccgc gctcattttg tgtcgcgcca tgacgcaatc gcggaacccg
     6721 ggcgcgaagc gctcaatttg cgcttcgatt cgttgcgaca tgtcgatgtt gcagtcgaac
     6781 ggcacatggc agtaggccca caaagtatgc tgcccttcag gtgcgcgcgt cgaatcaaac
     6841 agactttgtt gtgacacgag gacaaacggc cgctcgggaa tctcaccgcg cgcaacctgc
     6901 cgttcgctcc atgttatctc agcgaatgtg ccggcaagat gaacggtacc ggcgcgcttg
     6961 cataccgccg ctttccacgg aaccggatcg gacagcgcgt agtcgatttt gaaaacgccg
     7021 ggcgcatgcc ggaaattctc gagtcggcgg ccataacgat caggcaatcg gcttccgacg
     7081 agccgggcga aattccaaac cgaggtgtcg aaaagaacgg cgcacgaggg cggcagttgc
     7141 gcgaggttgc cgacgcggca gttgacgtcg atcctgccgc cgagctcgcg cagataagcg
     7201 gcgagggcat tcgcaatcga ctgcgacccg ccgcgcggaa tcggccagcc gaccgcgtgc
     7261 cctgccatcc caagcacaag cccaaaggcc gcggagaccg gtgcctccag aggcagaaac
     7321 gagtgcgcgg cgatcccgcc aaagagcgcc ctggccggct cagatttgaa aagcgttttg
     7381 acgagcagag tcgtcgggca catcgccgga atcccaaacc gcgccaaagc aaacggatga
     7441 cggggaaggt ggagcatcgg ttgcagaaat tcgggcgcga gcttttccca attccgcacg
     7501 agcggtttca tgagacggcc gtacctcgcc ccgtctcggc ctaacgaatc aatggtaaga
     7561 tcgacatctc gatgaaggca tgccgcactg ccatcatcga gcggatgggc cagcgggatt
     7621 tccggttgaa tccaatgaag cccgtgtcgt tcaagcggaa gcgatttgaa aaaaggcgaa
     7681 ccggcggcga gtgggtgcac ggccgaacag agatcgtgca aaaatcccgg caacgtcagt
     7741 tcggccgagc gcgcaccgcc gccgatcgtg gcgtttgctt ccaggacaag aacggaacaa
     7801 ccggcctgtg ccagcgtgat cgctgcggcc agaccattcg ggcccgagcc gattacgacc
     7861 gcatcccatt gctccgccat ttatcttccg agtagaagat ggccgagtca gtttccaagc
     7921 taaaacgcgc agaccagcga aagcttgcga attaattgta tgagcgcgcc acgcccccgc
     7981 aatcgccgac gcaatgccga tttttcagta gaaaaatacg gtgagcgaac gacagaaaaa
     8041 gaggaatgcg aggagcagtt gctcgccgaa tacgctgtgc tgctcgcgtt ttatttgacg
     8101 actgtggcgg ttttaaccgg tgcggcggtt gaacgtggtc ggctcccgcg aaagttcggt
     8161 ctgctcgatc tggccctgct cggcgtcgcg acccacaagc tgagtcgcat cgttgccaaa
     8221 gacagaatca ccgggatcgt tcgcgcccca ttcgtgaagt atattcgcag ctccggagcg
     8281 ggtgaggtcg aggaggaacc gcgcggtcgt ggtattcagc gtggcatcgg tcgtctaatt
     8341 tcctgcccgt attgcatggc cccatggtgc gggaccgcgc tcgggtttgg ttttgttttt
     8401 gcaccgcggg tcacgcggtt ctttgccgga attttcgcaa gcgtagctgt gtcggatttt
     8461 cttcagcgcg cttattcagc ggcgaaagag aagtggtagc cgcttagtgc ccagccgcgg
     8521 cggcagcgtg ctgctttagt ttccgcgagc tgcgtcgtcg gttcgacgac tcagacttgg
     8581 cccagtggcg attggttaac tgatggtgga aacagccggt aaatcctcct cctgctcttc
     8641 tccgattgcc cggcccggca attgcccgcg gtacgaaccc agttccttgc gatggaatat
     8701 tcggtaaagc accggcaaaa cgatcagcgt aagagcggtc gacgagatga tgccgccaat
     8761 gacgacggta gcaagtggcc gttgcacctc ggcgccgcgg ccatgcgcca gcgccatcgg
     8821 caagaagccg agggaggcaa caagggctgt catcaaaacg ggccggaggc gcgtgagcga
     8881 accgacacgg atcgcatcct ccagtgaatg gccgctttcg cgcagtcgtg caatgaagga
     8941 aaccatgacg acaccgttta gcaccgccac gccggagagc gcgatgaaac ccactcccgc
     9001 cgaaatggaa aacggcagcc cacgcaacca gagcgcgagg gttcctccgg ttaaggcaag
     9061 tggcacgccg gt
//
LOCUS       DASTDG010000127         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_402421, whole genome shotgun sequence.
ACCESSION   DASTDG010000127 DASTDG010000000
VERSION     DASTDG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402421"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1015
                     /locus_tag="VFO30_04050"
     CDS             215..1015
                     /locus_tag="VFO30_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9418492.1"
                     /translation="MKLRLGRGLLRPRSENAIHLIAALLLIAGSARAAEVIPPKPDRY
                     FNDYAGVVSKSAELRFNEQLAQFERETSDQVVVAVFPKMQSDSDIADYTQRVAQAWGV
                     GQKERRNGVVLFVFIQDRKMFIQVGYGLEGALPDATAFDITEYRIKPHFRSGDYEAGL
                     AVGIDSILQAIRGEYKGTGKTIAEQRRTGRAPGFVFFLFFIIFLIAISRVMRRLSGYG
                     YSSRRGGPVFVPMIGGGGGGWSSGGGGGFSGFSGGGGSFGGGGAGSSW"
     gene            1018..1452
                     /locus_tag="VFO30_04055"
     CDS             1018..1452
                     /locus_tag="VFO30_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9418493.1"
                     /translation="MRTREFLSKLEHDRIVQAIREAEAKTSGEIRVFIQRGKLSDDPL
                     IAAQKKFQRLGMHRTADHNAVLIFVAPRAHKFAVVGDKAIHEKCGEQLWQRLVDRMQV
                     HFRDEKFSDALVEAIEETGKALAAHFPKRSTSNELPDDLLEH"
     gene            1760..2791
                     /locus_tag="VFO30_04060"
     CDS             1760..2791
                     /locus_tag="VFO30_04060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9418494.1"
                     /translation="MPKTLVKDENTSTETYAKFTAEPFEAGYGHTVGNSLRRVLLSSL
                     EGAAITAVKITGAQHEFTTLPGVVEDVTDIVLNLKKVKFKVDGQDHEPKKLSLSVNKE
                     GEVTAGDIQAIQGFEVLNPKQVICTLDKKHKFDAELEVRVGRGFATGDENKRADQPIA
                     LIPIDSIFSPVTRVKYAVENTRVGQRTDYDKLILEIWTDRRLTPDDALLQASAILRHH
                     LDVFVNFNEDVIEFDETPAGVSEENIKLKKLLNMSVNEIELSVRAANCLNNANITTVG
                     QLAQKTEAEMLKYRNFGKKSLNEIKDKLQQLGLSLGMKFEAGLVETPAGTDGQTANGA
                     EATTAAERK"
     gene            2816..3391
                     /gene="rplQ"
                     /locus_tag="VFO30_04065"
     CDS             2816..3391
                     /gene="rplQ"
                     /locus_tag="VFO30_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9418495.1"
                     /translation="MRHQKKTVKLGRTAEHRKALLANQVCSLIVHQRIKTTLAKAKAV
                     RPLADKMVTLGKNGSLHARRNALSILRQSGAVKKLFDDIAPRSTSRNGGYTRIVKLGQ
                     RKSDSARMAFIEWVDAAVAVEEKPAEEKKAKRKEPKPEAKQKEPKREEPKREEPTRAE
                     APAKESEQAAPVPAPEKQKKRRWFGRKSEEK"
     gene            complement(3484..>3718)
                     /locus_tag="VFO30_04070"
     CDS             complement(3484..>3718)
                     /locus_tag="VFO30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9418496.1"
                     /translation="TRDRFKFRLQHIPPARPPTAVDVLLHGHTHVPRNEKRGGVLFLN
                     PGCVTRPNRGAAPSVAWLQIGHGKINWRLVPLR"
BASE COUNT          872 a         1078 c         1086 g          682 t
ORIGIN      
        1 aacctacaac actgcagtac gcagcttccc gaccaacctg attgccggaa tgttcggctt
       61 ttcgccgcgg ccattctttg ccgcccagcc aggcgcggaa cgaccgcccc cggtgcagtt
      121 taacttcggt tcgccggcgc ccgcgatctc accggtagcc acgccctaag taaatgtcga
      181 cctggggtgg atcgcgctct ccgagcgcga tgttatgaag ttgcggctgg gccgcggttt
      241 gttacgaccg cgttcggaga acgcgatcca ccttattgcc gcactcctgc tgattgcggg
      301 gagcgcgcgt gctgctgaag tcattccgcc aaaaccggac cgctatttca acgattacgc
      361 cggcgttgtt tcgaaatcgg cggagctgcg ttttaacgaa caactcgcgc aattcgaacg
      421 ggaaacgtcg gaccaggtcg tagtcgcagt ctttccaaaa atgcaaagcg actcggacat
      481 tgctgattac acccagcgtg tcgcacaggc gtggggcgtc gggcaaaagg aacgtcgcaa
      541 cggcgtcgtg ctgttcgtct ttattcagga ccgcaaaatg ttcatccaag tcggctacgg
      601 cttagaaggt gcgctgcccg acgccaccgc gttcgacatc accgaatacc gcatcaagcc
      661 gcactttcgc agtggcgact acgaggcagg acttgcagtg gggatcgatt ccatcctgca
      721 ggcgattcgc ggcgagtaca agggaacagg caaaacgatc gcagaacaac gccgcaccgg
      781 tcgcgccccg ggcttcgtat tctttttatt ttttatcatt tttctcatcg cgatttcacg
      841 agtaatgcgg cgactgagcg gctacggcta ttcatcgcgg cgcggtggtc cggttttcgt
      901 tccgatgatc ggcggcggtg gcggcggctg gtcctctggc ggcggcggcg gcttcagcgg
      961 gttcagtggt ggcggaggga gttttggcgg cggcggcgcc ggttccagct ggtaacggtg
     1021 agaaccagag aattcctgag taagctcgag cacgaccgta tcgtgcaggc gatccgcgag
     1081 gcggaagcga aaacttccgg cgagatccgc gtttttattc aacgcggcaa attgagcgac
     1141 gacccgctga tcgccgcgca gaagaagttt cagcgactgg gcatgcatcg aacggccgat
     1201 cacaatgccg tgctcatctt cgttgcaccg cgcgcacaca aattcgccgt cgtcggcgat
     1261 aaagccattc atgagaagtg cggcgagcaa ttgtggcagc ggctcgtcga ccgcatgcag
     1321 gtgcactttc gagacgagaa attcagtgac gcgctggttg aagcgattga agaaaccggc
     1381 aaagccctag ctgcgcattt cccgaaaaga tcgacatcaa acgaattgcc agacgacctg
     1441 ctcgagcatt gaggtgggat gcgcttccgg cgcatgtctc atcggcaaga tgccgatgcc
     1501 accaaactcc gcttgcaaaa ccggcgatct gctctttatt tagtgttccg ctgcgagttt
     1561 tgtaattacg ccgttggtcc ctgggtttcc gtcgctggac gcgaggccgc actcgccagg
     1621 gggaacccgg cgcgccgaaa ggatgaaggt tgaaggagga agggtgaaaa agccacaggg
     1681 ctgttttgtc ctttcataat tcatccctca tccttcatcc ctgaaatcac atatgccagt
     1741 tcgttacggt cgttttgaaa tgccgaagac tctcgtcaag gacgagaaca cctccacaga
     1801 gacatacgcc aagttcaccg ccgaaccgtt cgaagccggt tacgggcaca ccgtcggaaa
     1861 ttccctgcgg cgcgttctgc tttcctcgct cgaaggcgcg gccatcaccg cggtgaagat
     1921 cactggggcc cagcacgagt tcaccacgtt gcccggtgtg gtcgaggacg tcaccgacat
     1981 cgtgctcaac ctgaagaagg tgaaattcaa agtcgacggc caagaccacg agccgaagaa
     2041 gctttcgctc agcgtgaata aagagggcga agtcaccgcc ggcgacatcc aggcgattca
     2101 agggttcgaa gtgcttaacc cgaaacaggt tatctgcacg ctcgacaaaa aacacaagtt
     2161 cgacgccgag ctcgaagtgc gtgttggccg agggttcgcg accggcgacg aaaataagcg
     2221 cgcggaccag ccgatcgcgc ttatcccgat cgattccatc ttttcgcccg tcactcgcgt
     2281 caaatatgca gtcgagaata cccgtgtcgg tcagcgcacc gattatgaca agctcatcct
     2341 cgagatctgg acggatcgcc gcctgacgcc ggacgacgcg ctgctccaag cctccgccat
     2401 tctgcgtcat cacctcgacg tgttcgtcaa cttcaacgaa gacgtgatcg aattcgacga
     2461 gacgccggcc ggcgtgagcg aggagaacat caagctcaag aagctgctca atatgagcgt
     2521 gaacgagatc gagctcagcg tccgcgccgc gaattgcttg aacaatgcga acattaccac
     2581 cgttggtcag ctcgcgcaga aaaccgaggc ggagatgctc aagtaccgaa acttcggcaa
     2641 aaaatcgctc aacgagatca aggacaagct ccagcagctt ggcctcagtc ttggaatgaa
     2701 attcgaggcc ggactcgtcg aaacccccgc gggaaccgac ggccagaccg cgaatggtgc
     2761 cgaggcgaca acggccgcgg aacgcaagta acaactcgtc aggcgaactt cggagatgag
     2821 gcaccagaag aaaaccgtaa agctcggacg aacggctgag catcggaagg cgctactcgc
     2881 aaatcaggtt tgcagtctca ttgtccatca gcggatcaag actaccctcg ccaaagccaa
     2941 agccgttcgc ccgctcgccg acaaaatggt gacgctcggg aagaacggct cgctgcacgc
     3001 ccggcgcaac gcattgtcga tcttgcggca gagtggcgca gttaaaaagc tgttcgacga
     3061 catcgcgccg cggtcgacca gccgcaacgg cggttacacc cgcatcgtga agctcggcca
     3121 gcgcaaaagc gattccgcac gcatggcatt catcgaatgg gtcgatgccg ccgtcgccgt
     3181 tgaagaaaaa ccggcggaag aaaagaaggc caaacgcaaa gaaccgaagc cggaggcgaa
     3241 acagaaagag ccgaagcgtg aagaaccaaa acgcgaagag ccgacgcgtg cagaagcgcc
     3301 cgcgaaagaa tcggagcagg ctgcgcctgt tccggcgccg gagaagcaaa aaaagcgccg
     3361 ttggttcgga agaaaatccg aagagaagta acggttcagg tggatcgcgt tctcccaacg
     3421 cgatgtcgaa tcatggcggc aaagccgcat agcgttgaat caagctcgga gagcccggtt
     3481 catttagcgc agcggcacca gtcgccaatt tattttgcca tgcccaatct gcagccaggc
     3541 cacgctcggc gccgcgccgc ggttcgggcg agtgacgcag ccgggattca aaaacagaac
     3601 accgccacgt ttttcattcc gcggcacgtg cgtgtgacca tgcaagagaa catccacggc
     3661 cgtcggcggg cgcgccggtg gaatatgttg caagcggaac ttgaatcgat cgcgcgta
//
LOCUS       DASTDG010000128        26308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_690586, whole genome shotgun sequence.
ACCESSION   DASTDG010000128 DASTDG010000000
VERSION     DASTDG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 26308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26308
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690586"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3455)
                     /locus_tag="VFO30_04075"
     CDS             complement(<1..3455)
                     /locus_tag="VFO30_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9418497.1"
                     /translation="MTLLLRDYTFRTPLFLPAILLSTWYGGTGPGLLAVLLSTLSINF
                     FFLEPRFAFSFSFRDAVHLAVFLFSALLISSWSAARSRVEQALTRARDELEAKVKERT
                     AGLSRSNEQLRLENAERARAEGVLREKASLLNLTHDTVFVRDMNDVITYWNRGAEERY
                     GWSSEEAVGRVTHELTQTVFPAPLPEINAELLKTGRWEGELIHSRRDGTLVTVASRWA
                     LQRDESGNPVAILETNNDISERKQAEENLRHSEAFLAEGQRISHTGSWSWKVSNGNVT
                     WSDEHFRIFGFDPEKAEPSFQLFLETVHPEDRSFIERSLDEAVNEKKGFDLEFRIALA
                     DGSIKYVHGVGRPVLGKSGEVDRYNGTTVDISERKRGEALFAGEKRLLEMIATGVALE
                     EILNSLCLIIEDYRSGTLASVLLLRSDGTHLHSVAGPNLPKRWRQEMEKLPIGPCAGS
                     CGTAAYRGSAVMVSDIATDPLWELPEHRTAALSHGLRASWSNPILSSEGKVLGTFCIY
                     SHQPRSPSADDLGLIEKATYLARVAIERDRAEADLRTSEEKYRDLINASPDAICVIDA
                     DGKCLLVNPAAVALAGRSEDELIGSSIADTYLPEERHLLERRIDKLKAEGSFRFERQF
                     LRENGEIIPVEVSLSALRDRYYQSIIRDISQRKRREALLAGENRVLEMLAKGDSLSDI
                     LDNLCLLVEEQSSGVLASILLMDSNGKQLRHGAAPHLPKTYTEAIDGAFIGRAVGSCG
                     TAAYRAEQVIVSDITADPLWADFRDLALRHSLRACWSTPIFSSEGKVIGTFAMYYREP
                     RTPTAREQETIKHITHLAGVAIQRKLAETARRESEAYLAEAQRLSHTGSWAWTPATGE
                     IRYWSEETYRVLGFDPDAGPPRFEKFFGRLHPEDQDRVRELFGKAIAENADFETDYRV
                     VHPSGVVKDIHVVGHPVCDEAGHLVEFVGTVIDITESKRSEAALRASEQVARGQVEAL
                     AHSLDVLATAPDPQKFIGQMLSTIGRLLSAQSVSLWLFDEATDSLILRSATSVSGRST
                     ATHPEHPFMKNPLFWKENAVVQELLFTAGPLICDDIDTDPRVSGEWKEYLKRNGTRRF
                     LGVPLLVGGQVRGFVSIRHVDRASYRPEEVELTQALAHQVMLALQLNVFAEQGQRAAV
                     FEERNR"
     gene            complement(3552..4229)
                     /locus_tag="VFO30_04080"
     CDS             complement(3552..4229)
                     /locus_tag="VFO30_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET9418498.1"
                     /translation="MIPTNDDPQSREKDNQPMRLANVTLGQHRHVCAFFHGVEEEYRV
                     LLPFVKEGLGRGEKAFHIVDPTLRDEHVERLSSGGVDVTSVEKNGQFELHDWHEVYLR
                     DGHFDRDRMLACMQDVLEQSERDGFPLARVMGHAEWASGDWPGVDDFLEYECRLNDII
                     PKYKDAVICLYNLTKCGGNLIIDVIRTHPMILIGGTVQENPFFVPPAEFLRELRERRT
                     PQGKAAA"
     gene            4524..5033
                     /locus_tag="VFO30_04085"
     CDS             4524..5033
                     /locus_tag="VFO30_04085"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9418499.1"
                     /translation="MAGAARFPNMATRGPTPNEPMPDKRTRPRAMAAAEGLIVVNAPV
                     SDVYERWLAFEDYPKFITVIKRVRKLDANHFVASLRFHGKQYETTLEMMLRVQDRRLA
                     WRTFSDGRTPAHLATGVVSFVSDRDRMTRITLKLTSSFGGAVGRRIDRYLRNFKRLIE
                     EQIRESEDV"
     gene            5176..5859
                     /locus_tag="VFO30_04090"
     CDS             5176..5859
                     /locus_tag="VFO30_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9418500.1"
                     /translation="MKTESNGVKGTRLLLAVSNRLIRAGFRALLERIPWIEVVAEAGN
                     GREALALIATHRLDVLLVGMALRGLNGLEVSARVSKDFPKVKTVILSLYANKECVAEA
                     LRAGASGYLIKDAATVELELAIKTALNGAVYLSRGISKRAVDNYLKRAGNHVGPFALL
                     TPRQREVLQLIAEGNNTKAIAFALGISVKTVEAHRKQLMDRLSIHDVAGLVRYAMRFG
                     LVLSEIGSS"
     gene            6014..6703
                     /locus_tag="VFO30_04095"
     CDS             6014..6703
                     /locus_tag="VFO30_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9418501.1"
                     /translation="MRIRGLTQSTKEIRILIAGNHRLMRAGFRMLLETIPRIKIVGEA
                     GDGCEALELIRTRRPHVVIVGLGLSRLNGFEVIARLSKEFPEVKAILASLHAGKEYVA
                     QALLAGASGYLAKDTAPAELKLAIDAAMDGETYLSRDVSRRAVNAYVKNIGRKGAPLM
                     GLTPRQREILQLIAEGKNTKEIAFLLRLSVKTVEAHRKQLMNRLDIHDVVGLVRYAMR
                     FGLVPLETQRG"
     gene            6832..7683
                     /locus_tag="VFO30_04100"
     CDS             6832..7683
                     /locus_tag="VFO30_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9418502.1"
                     /translation="MEKFEPRPVGEVAQRITMNEALSRLQGHHELILNAVSDGILALD
                     VDESIMFANPRATEMLGWDAHELVGKPVHRALHHHPGEDTLYGTSSAIHNGMRDRASR
                     RVSNDVFWRKDGSSFHVDYTCAPMMDEHERVIGSIVIFKDVTDEVIGNSRLKLQAQQY
                     RLLFETNPSPMWVFDTKSLQILAVNEAALAEYGYSREQFLTLTVRDLRPAEDVSDFAR
                     EGMGPCVPSPFSGQFRHMKKDGSIILVEIYSAGIVWEGTSARIVTVINASERERTKQI
                     IKKRGGK"
     gene            8151..8528
                     /locus_tag="VFO30_04105"
     CDS             8151..8528
                     /locus_tag="VFO30_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9418503.1"
                     /translation="MQTTTEMDKRNNMRPTVEITEANFEVEVLKSSQPVLVEFATGWS
                     LASMMRDGALDEIATEFVDQLKVARVRLDSSPNLGLWYGIRCIPTMLCFVDGEERVGI
                     FGTTSKEAILSQLKPIMNSFAPV"
     gene            8555..8725
                     /locus_tag="VFO30_04110"
     CDS             8555..8725
                     /locus_tag="VFO30_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9418504.1"
                     /translation="MIRTISYLGLGVMGSGMASNLLKAGYELTVWNRNAEKCKPFARK
                     GARVAPNVPERL"
     gene            8770..8851
                     /locus_tag="VFO30_04115"
     tRNA            8770..8851
                     /locus_tag="VFO30_04115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8804..8806,aa:Leu,seq:tag)
     gene            complement(8927..9613)
                     /locus_tag="VFO30_04120"
     CDS             complement(8927..9613)
                     /locus_tag="VFO30_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9418505.1"
                     /translation="MLQKILVVDIGGTHVKLMIEGAEQRQFDSGSQMQPKQFVAELAD
                     QSGDWGFDALSIGFPSPVRDGKVVREPKHLGTGWVGFDFDAALNKPTRIINDAAMQAL
                     GSYHGGRMLFLGLGTGLGSALALEGMLFPLELGDLPYRDRDIIENFLGIPGISRLGED
                     EWKREVLFAITHLKLAFVADYVVLGGGLIHRFNDELPDGIERGKNENAFLGGLRLWEI
                     ENGRPKWQIF"
     gene            complement(9642..11939)
                     /locus_tag="VFO30_04125"
     CDS             complement(9642..11939)
                     /locus_tag="VFO30_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418506.1"
                     /translation="MTANDAPPKEGRVMVLEPEEQVASAILSAVHEAAPAAQVDMAHS
                     LQEAQQLVVGTKPDLFVLDIDATQDLGQDFLYDLRTSHPNARAIILTATHLPAQREQT
                     AGLGAIHFLEKPFPHRDFVDLAEALLRAPDRAAEKFRGTLSDLHIADVIQLKCMSGAT
                     SVVEFTGPRGEKARVFFEKGQVRHATAPGTQGIAAFNEIVSWKGGTISEVSGAAPAPR
                     TIDQDWQFLLMEAARKSDEHAAAGESKKKPRAESVKRKVLVIDDSLMLLSFVEEVLAE
                     ANYEVRTASAARTGLDACKAERPDLVLLDYILPDMKGDEVSRRLLDDPTTADVPVVYM
                     SGYGADLKREGANPRNVIGFLNKPFTSDLLVETVEEHTSQSRGEVEPAEAAGPDTELL
                     SAAKTDLPTNTDSAAPAVEELAEPETTDLFQEQAEEPPRTPEATPAEVTSSQASPPTP
                     IETDMAPLPSEDLWAEIPPPDSTAPTVIEPMPRPEPTPTAAAEVAVADESVTGGPFFC
                     GDTRFFSLNRALQTIRKQKLTGWLRAYWPKAPVELLTRNGEIVLVTTRDAQLYCPDEP
                     ITLVNVDPERIVAARSDQGLSGCPLFLTLAEQELILREPAVQLVQHYGQKLFAQLWTA
                     PHVRFLFERSSDYPEYATDLPSEADLDHWALASLRFLQFQDLGDHGNIEVACIPAYTK
                     DGFERAQNLKLTVAEAQFASQFNGSRSVQQIARNLRLDLKFARLTLFRFLALEIVECW
                     PATAAVKPEKTGIFQRLTRSIGMGE"
     gene            complement(12065..13399)
                     /locus_tag="VFO30_04130"
     CDS             complement(12065..13399)
                     /locus_tag="VFO30_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HET9418507.1"
                     /translation="MRWDDPLLAAWEETLARKRDAPAIFNTRGEVVRTFRQIEERARA
                     DEAKFPTVKPHNVNAIQIGNHENWPSIFLACLRTQRVVLPIDESVGKEQADTAVSIAA
                     KSGIVDWGDKPPSLFKLTSGTTARPRLVRFRSGQLLADCDQICETMGIEDVDLNFGVI
                     PISHSYGFSNLLTPLIARGVPLVLSRDRTPRALLADIARTNATVFPGTPAFYQALAEI
                     DDAPVLPRLRLCISAGAPLSVSVARKFQQKFSLPIHSFYGASECGGICYDREAANDID
                     GFVGQPMKHVDVAMIDDNAQVSQVRVRSATVGDGYFPDANEEKLGNGVFVPDDLLTKT
                     AQGFRIVGRVSDVINVAGKKVHPAEIEAQLRRFAGVREAIAFGRESTLRNEEVAACVV
                     GNTTVTEDALLEFCRQHLSSWQVPRRIFIVDELPVNERGKISRRELARQFVS"
     gene            13451..13678
                     /gene="rpmE"
                     /locus_tag="VFO30_04135"
     CDS             13451..13678
                     /gene="rpmE"
                     /locus_tag="VFO30_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9418508.1"
                     /translation="MKAGIHPEYYETDIVCACGAVYHTRSTRRDIKIGICARCHPFFT
                     GEQKFVDTAGRVEKFARRYGSTKAARGGKKA"
     gene            13746..14822
                     /gene="prfA"
                     /locus_tag="VFO30_04140"
     CDS             13746..14822
                     /gene="prfA"
                     /locus_tag="VFO30_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9418509.1"
                     /translation="MDFDSLIQRKREQFEELEREIADPALFSNRKRAGEVMREHASIK
                     QVLAKWNELEQARKELDDTRELALSNDVAMAAMAEDEIVELETRVLDLERELQIALVP
                     RDEHEERDAIVEIRAGTGGNEAAIFAADLYRMYNRFAEANGLKTEDLESSPSELGGLK
                     EVIFRVSGESVFQKLRYESGVHRVQRVPATEAQGRIHTSTATVAVLPEAEDVDLELKP
                     EDLRIEVSRAGGPGGQGVNTTDSAVQVLHIPTGTMVRCQDGRSQIKNKERALSILRAR
                     LLERKQREEAEKYSAQRRGQIGTGGREEKIRTYNFPQNRVTDHRIGLTLYNLDRIMDG
                     DLNELTTALQTADMGERLKDSSVA"
     gene            14819..15655
                     /gene="prmC"
                     /locus_tag="VFO30_04145"
     CDS             14819..15655
                     /gene="prmC"
                     /locus_tag="VFO30_04145"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9418510.1"
                     /translation="MTLLEVLQKSTDFLAKRKVENARLNAEHLLGHVLGRTRMELYLE
                     FERELNEHELAPLRDLLRRRGEREPLQYLLGTVEFCGETFLCDKRAMVPRPETEELVE
                     FLRSEIRNPKSEILDLGTGSGVIALSLAKQFPEAEVVAADMSDEALALARENAERLGL
                     SERVQFLKSDLLANVFGTYELIVANLPYVATQDRQSLSREVLHDPEIALFGGERGDNL
                     IRQLIDQAPLHLKPGGLLALEIGIGHAASLSQVLAEKKYHDIAAKNDYSGVTRFLLAR
                     YG"
     gene            15648..16907
                     /gene="murA"
                     /locus_tag="VFO30_04150"
     CDS             15648..16907
                     /gene="murA"
                     /locus_tag="VFO30_04150"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9418511.1"
                     /translation="MDKIRIQGGQALSGSIKISGSKNSALPILAATLLTREQCIIHRV
                     PDLSDTHYMLQILTHLGAQVERASGIVCVTAEKVNSVAPYDVVRKMRASICVLGPLLG
                     RCNEATVSMPGGCVIGDRPIDLHLKGFEALGAAVRVEGGNIKVFAPKLRGAVINMVGR
                     FGPSVLGTDNAMMAAVLAEGTTVIDGAAQEPEVVDLANFLIQMGARIEGAGTRRLIIE
                     GVPELHGAEYEIIPDRIEAGTFLVAGAISGKRVTVKRVEPEHVRAVTDALRRCGFAIE
                     TADDAISICPNGAAQPLEFSTEPYPGFPTDMQAQMCALLSTTDGRSVVRENIFPQRYM
                     HVAELKRMGAQMELDGATALINGVDHLYGAPVMASDLRASAALVLAGLKADGVTEVSR
                     VYHIDRGYEHLDEKLRELGADIERVKG"
     gene            16911..17285
                     /locus_tag="VFO30_04155"
     CDS             16911..17285
                     /locus_tag="VFO30_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9418512.1"
                     /translation="MNETPPPLPATSASSVRTWCVLAHALALIGFFVPWAGHIIGPLI
                     VWLAKRGDSPEIEAHARESLNFQLSMLIYNAVAALLCLVLIGLLILPILHIINVILVI
                     VASIRASEGKLYHYPLTIRLIS"
     gene            complement(17282..18181)
                     /locus_tag="VFO30_04160"
     CDS             complement(17282..18181)
                     /locus_tag="VFO30_04160"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9418513.1"
                     /translation="MRDVSTSLDMTKQILLGAHMSIAGGVHMAIERACSIQCTAMQMF
                     VKNNMQWFARPLTREEIRAFCNHVQRGELLSIFAHANYLINLAATNPQFHANSLRALA
                     EELTRSNQLELPFLVLHPGAHLGAGEEAGLEKIIASIDAAFRVLPKVKTKIALETTAG
                     QGSCLGHTFEQIAYVIDNVRQPERLCVCLDTAHVFAAGYDISSEPAVRKMFREFDRTI
                     GLNRLAAIHVNDSKAPRGSRVDRHEHIGKGKIGLNAFRAILRDCRFAKIPKVLETPKE
                     KDLRQDIENLKTLRGLIEIKPPR"
     gene            complement(18228..20621)
                     /locus_tag="VFO30_04165"
     CDS             complement(18228..20621)
                     /locus_tag="VFO30_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET9418514.1"
                     /translation="MPAVLETPPEVTRRRPQDRQPSLARRFVRSAVRLAVVLALSAFI
                     TGGWYLSKKGFGREWRSRIAEELHKHGVEAHIRRLTLDPFRGLIAKDVRIFDYKRREN
                     TLARISEIALDINYAALLHHQPFLNALDIRNAQITLPIKTSDTGSAQAQLTNFRAHIY
                     FPPEQIYVSQAEGIFCGLRISATGQLIKRTSYQPSSALTEEEWRMRLSMLQRVAIELQ
                     KFNFPRARPSLQVKFSGDLEQLEDAHLEATLQGEELRRGAYTISELFASAEWTDQVFN
                     ISRCQWRDIVGTFAAKASWGRRTNEANFQIRSDLALKDFLDAFGLGQPLRDAAFTAPP
                     ALELSGVASFGGERPQIKVIGRASVGAFSYKGVPFADLRGQFSWDGERTLLRDVRLRH
                     QTGDLSAQLFDAPNDFRLKIDSHLSPAILRPLVSPELSQFLSEWEWPRPPDVHLVIHS
                     QDRQPENWKGEGTIALERARFRGAWMNSATANVRFGDGAILYDNLRVTRDEGIGTGSF
                     AYDFKNHEVRINKIKTSLRPAEAILWVDPKLWKTVVPYKFRRPPNVTTNGVYQFRGGK
                     NTHIDIGVEAPGGMDYGFLGKTLPFDRVVSRLLFTNDRLQIVDLKAGLFSGSVHGTAD
                     ISLAKNDPHYHAKIAVAEINFPQLTDLYYNYKTAQGALSGTYDFTGLGTAARAMQGSG
                     TLEVTNGDVFAIPVFGPLSAILNSIIPGAGYSIAHRADARFTIKDGIIHTDDFDATGR
                     LFRLLGHGDIHFLDDKLDFYLRIGGKGPSILLTPVYKLFEYVGEGSLKHPDWHPKRF"
     gene            20702..21139
                     /locus_tag="VFO30_04170"
     CDS             20702..21139
                     /locus_tag="VFO30_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET9418515.1"
                     /translation="MISISDLPAINASLNLLSTIFISAGWFWIRRNIWQRHVPCMIAA
                     IITSTCFLVSYVVYHAHAGEKSSGYTGWLAAIYLPILASHVLLAFITLPLVVVTLVPV
                     FQRRWDRHRRLGRWTMPIWLYVSVTGVLVYLILYHWFPPANLK"
     gene            21233..21718
                     /locus_tag="VFO30_04175"
     CDS             21233..21718
                     /locus_tag="VFO30_04175"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET9418516.1"
                     /translation="MISSAPHEANGTMVTIATFNEPAKARQIKQRFQQAGVRADIHNE
                     APLQQVGFMSRPQANAKVMVDDDEFERAQTLMIEWEANDAEIAAAIVRCPQCGSPQIE
                     YPQMTRKFITPAMVGLLCALRIIPKEFYCQDCHFTWSNEEERTIGRLWHRFFPGAEQT
                     S"
     gene            21792..22628
                     /locus_tag="VFO30_04180"
     CDS             21792..22628
                     /locus_tag="VFO30_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9418517.1"
                     /translation="MKWLIALLLALLIFGGAWIGYNVVFHEDIQVRKEERGEVAPAPT
                     PDISLPEFQAAAKLRQDGKLIEARDALSAFILKYPSGLHTEEAKDLLGEINVDILLSR
                     IPSPEKEEYIVKPGDVLAKISHKLKTTPELIMRMNNMSSTMLHIGERLLISHPDLSMV
                     IERKGKLVVLLNHGAFFKQYHVLEEKLSPRQPPKVNTKVAEIMAWRDGRRVGFGTKEF
                     IGSTRWIRLGTPAYTIYSVPDSAHPNVGQPPPAQGIGLAATDVEELSGLVNNRTPVTI
                     TD"
     gene            22720..23706
                     /locus_tag="VFO30_04185"
     CDS             22720..23706
                     /locus_tag="VFO30_04185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET9418518.1"
                     /translation="MDLQPLDFEKPLFELERRLRDLKTHSDEHDVDLDEAVKAIETKI
                     TETRGQIYGKLTAWQRVQIARHVQRPFALDYVERCFTNWVELHGDRRFADDKAMPCGM
                     AMFGTQRCVVLTHQKGRNTKENVMRNFGCAHPEGYRKALRLMRLAEKFGMPVISLIDT
                     PGAFPGIGSEERHISEAIAVNLREMMNLRVPIIAAVIGEGGSGGALGIGVADRVLIFE
                     NAYYSVISPEACSAILWRDRRHAAEAAEALKLTAHDLLNLGVVDEIVPEPEGGAHHDY
                     NVAAANLGAALRKNLEGVRALVIDELIEKRYDKFRELGNFEEAPSAATAARA"
     gene            23734..24510
                     /locus_tag="VFO30_04190"
     CDS             23734..24510
                     /locus_tag="VFO30_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418519.1"
                     /translation="MIAPLVLLVVAHLPNADAAPDKFSYARDTFAFANATVFKYYRGI
                     AFLRSPSESDRKLYTRRCFVMTRGAVQFYKFARFDPNGQPLDDRELARRIRAVSRRAP
                     WHAPLPDSQRVVFPGYANLNDMSKARTKVVQRNIGLGWPTYMRLGNFRMLFQHDPRYQ
                     EKTHTNLNAALDRGDLFIGYLSTFPSLAINHSILVYARKPERPDHDVEQHYLAYDPNH
                     PDGPRVLTWSARNRAFAYQKDSDFIGGFVRVYQIYGKLLQ"
     gene            complement(24671..25480)
                     /locus_tag="VFO30_04195"
     CDS             complement(24671..25480)
                     /locus_tag="VFO30_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9418520.1"
                     /translation="MAEHTHTGNIKRHRGFASRILGNRRDVLVYLPPGYRRFSRTRYP
                     VLYLQDGQNVFDAATAFGGVEWRVDETAERLIRKNLIEPLIIVAVANMGEDRVHEYAP
                     TRGVLSEKAARKKRSKGLGRKYGEFLIGELKPYIDRKYRTRRDAEFTGIGGSSLGGLL
                     ALSLGLWFPNIFTKLIAMSPSVWWDNEVIVQMVDDLDRKLPHKIWLDTGTQEEAWERV
                     RHLRDALVEKGWILHRDLQYSERRGGEHSEAAWAERVDPALRFLYPPLRRR"
     gene            25577..26287
                     /locus_tag="VFO30_04200"
     CDS             25577..26287
                     /locus_tag="VFO30_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9418521.1"
                     /translation="MHERYIKWYTQWLSRDFEMLIFGHGGGLPLILFPTSFGRYYQTK
                     DFGLVDSVAPFVDSGRVTVYCPDAIDLESFYNKSIHPADRMRTHNAYERVIVNDVFEF
                     ARRECSAHRVAMSGASLGAYHAANIAFRYPDLVGALISLSGSFDIRSFFDGYHDDNIY
                     FNSPYEYLPNMPDPWKYNHMRIIIGTGEWDNTRHESLRLAEILNSKGIPHWLDDRKWC
                     GHEWSYWRDMLPYYLSTL"
BASE COUNT         5642 a         7634 c         7488 g         5544 t
ORIGIN      
        1 cgattgcgtt cttcgaagac cgcggcccgc tgcccttgct cggcgaacac gttgagttga
       61 agcgcaagca tgacttgatg ggccagtgcc tgggtcaact cgacttcctc cggccgataa
      121 gacgcacggt cgacatggcg aatgctgaca aaacctctga cttgtccgcc gaccaaaagc
      181 ggcactccga gaaagcgtcg ggtcccattt cgtttcaaat actccttcca ctcgccactc
      241 acgcgcggat cagtatcgat gtcgtcgcaa atcagcggac cggcggtgaa aagcagttcc
      301 tgaacgactg cattctcttt ccagaataac gggttcttca tgaatggatg ctccggatgg
      361 gtcgcggtcg accttccgct aactgacgtc gcggagcgca aaatgagcga gtcggtcgct
      421 tcgtcgaaca accacaagct tacgctttgc gcgctcaaga ggcggccaat ggtactcagc
      481 atctggccaa taaacttctg gggatcaggc gcagtcgcta aaacatccag actgtgtgcg
      541 agcgcctcga cctggccgcg cgctacctgc tcggaagcgc gcagcgccgc ttcggagcgt
      601 ttgctttcag tgatatctat caccgtgccc acgaattcca cgagatgccc ggcctcgtcg
      661 caaaccggat gaccgaccac gtggatgtcc ttaacgacgc cgctcgggtg aacaactcgg
      721 tagtccgttt caaagtccgc gttctcggcg attgcttttc cgaacagttc tctgaccctg
      781 tcctgatcct cagggtgaag tcgcccaaag aatttttcga atcgcggcgg cccggcgtcc
      841 ggatcgaagc caagcacgcg gtaggtttcc tccgaccagt atcggatctc gcccgtagcg
      901 ggcgtccacg cccaactccc ggtatgactg agtcgctgcg cttccgccag atacgcttcg
      961 ctctcgcgcc gggccgtttc cgccagctta cgttggatgg cgacacccgc gagatgagta
     1021 atgtgtttaa tcgtttcctg ctcacgcgcg gtcggggtgc gtggctcgcg atagtacatg
     1081 gcaaacgttc cgatcacttt gccctcggag gagaagatcg gtgtggacca acaagcgcga
     1141 agcgaatgtc ttaaagcgag gtcgcgaaaa tcggcccaca gaggatccgc agtgatgtcg
     1201 gacacgatta cctgttcggc gcggtaggca gccgtaccac atgaaccgac cgcacgccca
     1261 atgaaagctc cgtcgatcgc ttcggtgtaa gttttcggaa gatgtggtgc cgcgccatgc
     1321 cgcaactgtt ttccattcga atccattaac aagatggagg ccagaacacc ggaagattgc
     1381 tcttccacca gcagacataa gttatcgagg atatcggaaa gcgaatcgcc tttggccaac
     1441 atctcaagaa ctcgattttc cccggccagg agcgcttcgc gccgcttacg ctggctgatg
     1501 tctctgatga tcgattggta atatcggtcg cgcagcgctg acaaagaaac ctccaccgga
     1561 attatctcgc cattttcccg caagaattgt cgctcgaacc gaaaagagcc ttccgctttc
     1621 aatttgtcga tgcggcgctc caagagatga cgctcttctg ggagatacgt gtctgcgatt
     1681 gaactgccaa tcaattcgtc ttcggaacgc cctgccagtg caacggccgc tggattcact
     1741 aacaaacatt tgccgtccgc atcgatgacg cagattgcgt ccggcgaagc gttgattaaa
     1801 tcccgatact tttcctcgct cgtgcgcaga tccgcttctg cccgatcgcg ttcaatcgca
     1861 actcgcgcta ggtaggttgc cttctcgatc agtccgagat catccgcgct cgggcttcgc
     1921 ggctggtggc tgtaaatgca gaaggttccc agcacttttc cttccgaaga aagtatcgga
     1981 ttcgaccagg aggctcgcag cccgtgactt aatgcggccg tgcgatgctc cggtaattcc
     2041 caaagcggat cagtcgcaat gtcggagacc atcaccgccg atccgcgata tgccgccgtt
     2101 ccacacgaac cggcgcacgg tccaatcggc agcttttcca tctcctgtcg ccatcttttc
     2161 ggaaggttcg ggccggccac cgaatgcaga tgagtgccat cggagcgaag caacaagaca
     2221 gacgcaagcg tcccgctacg ataatcctcg ataatcaggc agagggaatt caggatctcc
     2281 tcgagcgcga caccggtagc gatcatttcg aggagacgct tttcgccagc gaacaacgct
     2341 tctccgcgct tgcgttcgct gatgtctaca gtggtgccgt tataacggtc gacttcgcca
     2401 gacttcccaa ggacgggtcg gcccacaccg tggacgtact taatcgatcc gtccgcgagt
     2461 gcgattcgaa attcgaggtc gaatcccttt ttctcgttaa ctgcttcatc gagacttctc
     2521 tcgataaacg atcgatcttc cggatgaact gtttccagaa ataactggaa ggatggctcg
     2581 gccttctccg ggtcgaaacc aaagatgcgg aaatgctcgt ccgaccaggt gacgtttcca
     2641 ttcgaaacct tccagctcca gcttccggtg tggctgattc gctgtccttc ggccaaaaat
     2701 gcttcgctgt gccggaggtt ttcttcggct tgtttgcgct cgctgatgtc attgttggtc
     2761 tcaagaatcg cgacaggatt gccggattcg tcccgttgca gtgcccatcg actcgccacc
     2821 gtgaccagcg ttccatcgcg gcgactgtga atcaattcgc cctcccagcg gccagtcttc
     2881 agtaattcgg cattgatttc cgggagcggc gcggggaaca cggtctgggt gagttcgtgc
     2941 gtgacgcggc cgacagcctc ttcgctcgac cagccgtatc gttcctcggc gccgcggttc
     3001 cagtaggtaa tgacgtcgtt catgtcgcgg acaaagacgg tgtcgtgagt gagattgagc
     3061 agacttgcct tttcgcgcag gacaccctcg gcccgggcgc gctcggcgtt ctccaaccgc
     3121 aattgctcgt tgctccgact aaggccggcc gttcgctctt tgactttggc ctccagctca
     3181 tcgcgtgctc gcgttaaggc ctgctcaacg cggctacgcg cggcgctcca cgagctaatc
     3241 agtaaggccg aaaaaagaaa aacggcgaga tgaaccgcat cgcgaaaact aaatgaaaag
     3301 gcgaatctcg gttcgagaaa gaaaaaatta atcgataacg ttgagagtaa cacggcgagc
     3361 aagcccggtc ccgtgccccc gtaccatgta cttaacagga tcgcgggcag aaagagcggc
     3421 gtgcggaacg tgtaatctcg caggagaagg gtcacgctcg ttgagagcgc gaccaataaa
     3481 acagagagac cgtaccgcca aacggccgga gcaggtcgtc gcaaaccaag atctgacttc
     3541 gacatttagc ttcaggccgc tgctttaccc tggggcgtgc ggcgttcgcg cagttcccgc
     3601 aaaaactcag ccggcgggac aaagaacggg ttctcctgga cagtaccgcc gatcaggatc
     3661 atcgggtgag ttcggatgac gtcgatgatt agattgccgc cacacttcgt cagattataa
     3721 agacagatta cggcatcctt atatttcggg atgatgtcgt tcaatcggca ctcgtattcg
     3781 aggaaatcgt ccaccccggg ccagtcgccg gaggcccact cggcgtggcc catgacgcgg
     3841 gcgaggggaa atccgtcgcg ctcgctctgt tccaatacgt cctgcatgca cgcaagcatt
     3901 cggtcccggt cgaaatgacc gtcgcgcaaa taaacttcgt gccaatcgtg cagctcgaac
     3961 tgtccattct tctcgaccga agtaacgtcg acaccgcctg aggaaagtcg ctctacgtgc
     4021 tcgtcgcgca gtgttggatc gacaatgtga aacgccttct cgccgcgccc gagcccttcc
     4081 ttaacgaacg gaagtagcac ccgatactct tcctccacgc cgtggaaaaa cgcgcagaca
     4141 tggcgatgtt ggccgagcgt cacattggca agccgcatcg gttggttgtc cttttcgcga
     4201 gattgagggt cgtcgttagt cggaatcata gggatttcct ttcctcgttg agccgcgtcc
     4261 tgacttgaac aaccgagtac tctaacaggc gtccacgctc tccgccagac taacgtcctc
     4321 cctcgaaaga ggcactctaa aatcccgcca ctgcaaggtg gtgacaggcc gcccgcctat
     4381 tagcattaga gagacaaaaa tcaccatgaa agctaaacca cgtgaaaaca atttcagaaa
     4441 ccgaatacgc aacctttttc ggcgacgcgc gcccaggcat gaccccttac tcgtcgtcaa
     4501 catgtcgatc tttaatttta ccgatggctg gagccgcacg ttttccaaac atggctacgc
     4561 gcggtcctac gcctaacgag ccgatgccgg ataaacggac gagaccgcgc gcgatggctg
     4621 cggcggaggg attaatcgtg gtcaacgcgc ccgtttccga tgtctacgaa cgctggctgg
     4681 cgtttgagga ttacccaaaa ttcatcaccg tgatcaaacg cgtccgaaaa ttggacgcga
     4741 accattttgt cgcatctctc cgtttccacg gaaaacaata tgagacgacg ctcgagatga
     4801 tgctgcgtgt tcaggatcga agactggcct ggcgaacttt ttccgacggt cggacacccg
     4861 cccaccttgc taccggggtc gtttcgtttg tttccgatcg tgatcgaatg acacggatca
     4921 cgctcaagct aacctcaagc ttcggcggcg cggtggggcg gcgaatcgat agatatctgc
     4981 gcaatttcaa acggttaatt gaagagcaaa tccgcgaatc ggaagacgtc tgagtacttg
     5041 cggacgctga ttgaacttct gacagtcgcc cgcgtctctt cgccagtttt acgccctccc
     5101 tcgaaggagg tagcgacaaa tcctgcgact gctgcttggt cacggccggg gatacagttg
     5161 atattgtatt gcataatgaa aaccgaatcg aatggcgtaa aaggaacgcg gcttttgctg
     5221 gcggttagta atcgtcttat ccgtgcaggt tttcgggcgc tgctggaacg aattccgtgg
     5281 atcgaagtgg tcgctgaagc cggcaatggg cgggaggctc tagccttaat cgccacccat
     5341 cgattagacg tcttgctcgt gggaatggcg ctgcgaggac ttaacggtct ggaagtgtcg
     5401 gcccgtgtaa gcaaagattt tccaaaagta aaaacggtca ttctctcgct ctacgcgaat
     5461 aaagaatgcg tggccgaagc gctccgcgcc ggcgcttctg gctacctgat caaagacgcc
     5521 gcgacggtgg aattagaatt ggccatcaag actgcgttga atggcgcggt ttatctcagc
     5581 cgaggaatct caaaacgtgc ggtcgataat tatctcaagc gcgcaggcaa tcacgtcggg
     5641 ccgtttgcgc tgttaacacc gcggcagcgc gaagttcttc aactcattgc cgaaggcaac
     5701 aacacaaagg caatcgcatt cgctctcggg atcagcgtca aaaccgtgga agcacatcgc
     5761 aaacagttaa tggaccggct cagcattcat gatgtcgctg ggttggtgcg ttacgcaatg
     5821 cgtttcggcc ttgtgctatc cgaaatcgga tcaagctgag agttttcgac acgtagctct
     5881 gagcttcgtc agtaaggttt tccctaatac ccgccaccac ggctcgcgtt gtactaaaca
     5941 cgatgccgat ggaaaccgat ccccaaaaaa ccgtttgcga caaacaaacg ccctcagccc
     6001 tgctcgacag ccagtgagga ttcggggact tacgcagtcg acaaaagaaa tccgcatctt
     6061 gatcgcgggc aatcatcgtc tgatgcgggc cggttttcgg atgttgctcg agacaattcc
     6121 ccgaataaaa attgtgggag aagcgggtga cggctgcgaa gcgctggaac ttatccggac
     6181 gcgccgtccg catgtggtaa tcgtcggcct tggcttaagt agactcaacg gtttcgaggt
     6241 cattgcgcgc ctgagcaagg aattcccaga ggtgaaggcg attcttgcgt cgttgcacgc
     6301 cggaaaggaa tacgtcgcgc aggcccttct cgcgggtgct tctggctatc ttgccaaaga
     6361 tacggcgccc gctgaattaa agcttgccat cgacgcggca atggatggtg aaacctatct
     6421 cagccgcgat gtttcccgac gcgccgtcaa tgcctacgtc aagaacatcg gccgcaaggg
     6481 agctcctctg atgggactca cgccgcggca gcgcgaaatt ctgcaactga ttgccgaggg
     6541 caaaaacacg aaagagatcg ccttccttct tcgcttgagc gttaagaccg tggaggcaca
     6601 ccgtaaacag ttaatgaacc gactggacat tcacgatgtt gtcggtttgg tgcgttacgc
     6661 gatgcgtttc ggtctcgttc cgctggaaac tcaacgcggt tgaccgggtg ttatagcggc
     6721 ggttgtgagc cgacccgccg agcctctaag gttttccctt atggcgaccg tctcgttagg
     6781 cggtaggttt gcgcaccagg caaacgcgaa tccgttaact tgggcagcga catggagaaa
     6841 ttcgaaccac ggcctgtggg cgaagtggct cagcgcatta ccatgaacga agctttgtct
     6901 cgattgcagg gccaccacga gttgatcctc aacgccgtca gcgatggcat tctggctctg
     6961 gatgttgacg aaagtattat gttcgccaat cccagagcga ctgaaatgtt gggatgggac
     7021 gcccatgagc tggttggcaa accggttcac cgcgcgcttc atcaccaccc cggcgaggac
     7081 actctttacg gcacgtcttc cgcgattcat aacggcatgc gcgatcgggc gtctcgccgc
     7141 gtcagcaatg acgtcttctg gcgaaaagat ggttcatcct ttcacgtcga ttatacgtgc
     7201 gctccgatga tggacgaaca tgagcgggta atcggaagca ttgtcatatt caaggacgta
     7261 accgacgagg ttatcggcaa ttcgcgcctg aaattacaag cgcagcagta ccgcttgtta
     7321 tttgaaacaa acccgagccc gatgtgggtt tttgacacaa agagtctgca aattctggcc
     7381 gtgaatgaag ccgccctcgc cgaatacggc tattcccgag agcaattcct gacgttaaca
     7441 gtgcgcgatt tgcggccagc cgaagacgta tccgatttcg cccgagaagg gatgggaccg
     7501 tgcgtgcctt ctccttttag tggtcaattt cggcacatga aaaaagatgg atcgatcatt
     7561 ttggtggaaa tctattccgc aggaattgta tgggaaggta cttcagcacg gatcgttacg
     7621 gtgatcaatg cctcagagcg cgagcgcaca aagcaaatca tcaagaagcg cggcggtaag
     7681 tgaagaacag caagtttcat gagcaaagcg tgcgaatctt gatcgctgac gatcatgaat
     7741 tactgcgggg agatgcccgc cagagtgatc gaaccgaaac gcgttaggac aaaaaaagtt
     7801 cgcgtcctcg ccacgaatct gaagaggtct gatcactggc tttgaggatg aagactccct
     7861 cgaacgaggg agcgccgacc accccggtcg aggcgactca aaatcccact cacgcgcagt
     7921 ggccgcggcg taatcaccaa cggtacgttg gccaatgccc aacgaaattc attcttaccg
     7981 cgacctgcag cagcagatcc accaagattt gcgggctcag catccggaat gggttcaacc
     8041 taacggtgac tgccctatct gcgactctta cgagacgcgt ctggcggagc tactcggcct
     8101 cactcagcgc tcgaatgcgg ttggcatgca accgtcactg tctttcagtc atgcagacga
     8161 ccaccgaaat ggacaagcgc aataatatga gaccgactgt tgaaattact gaagctaact
     8221 tcgaggtcga agttctgaaa tccagtcaac cggtgctggt cgagtttgcc accggatgga
     8281 gccttgcttc catgatgagg gacggagctc tggacgaaat tgccactgag tttgtcgatc
     8341 agctgaaggt agccagggtc agactcgata gcagccctaa tctcgggctg tggtacggta
     8401 ttcgatgcat tccgacgatg ttgtgcttcg tcgatggcga agaacgcgtc ggaatttttg
     8461 ggacgacgag caaagaagca attctttctc agttgaaacc gatcatgaac agcttcgcgc
     8521 ccgtgtaaat gaacaatctc cgcgacattc aggtatgata aggacaattt cttacctcgg
     8581 tcttggcgtc atgggcagcg ggatggcgtc caaccttctc aaagccggct atgaacttac
     8641 cgtctggaat cgcaatgctg agaaatgcaa accattcgcc aggaaaggcg cgcgggtcgc
     8701 tcctaatgtt ccggagcgat tgtgaactcg gatttgtgct aggtcgacaa tccttcataa
     8761 tcaaatggcg ccagggtggc gaaattggca gacgcgccag acttaggatc tggtggagca
     8821 atccttaggg gttcgagtcc cctccctggc aattaacagg aagaaaacgc tcaatgtcga
     8881 attcagacgg cgcgagttcg gcgttggagg ttcggcgttt gctgcttcag aaaatctgcc
     8941 atttgggccg gccgttctct atttcccaca gccgtaagcc gccaagaaaa gcattctcat
     9001 ttttcccgcg ctcgattccg tcgggtaact catcattaaa gcgatggata agcccgccgc
     9061 ccagcacgac atagtcagca acgaacgcga gcttgagatg cgtaatggcg aagagcacct
     9121 cgcgtttcca ttcgtcttca ccgagccggc tgatgccagg gatgccgagg aaattctcga
     9181 tgatgtcgcg gtcgcggtaa ggtaaatccc cgagctcgag cggaaagagc attccttcca
     9241 gcgcgagagc cgagcccagt ccggtgccga gcccgagaaa caacatgcgt ccgccgtgat
     9301 aactgccgag tgcctgcatg gcggcgtcat tgatgatgcg cgtcggtttg tttagcgctg
     9361 cgtcgaaatc gaagccgacc cagccggtgc ccagatgttt cggttccctg acaaccttgc
     9421 cgtcgcgcac cggtgatgga aatccgatcg agagcgcgtc gaatccccaa tcgccgcttt
     9481 gatcggcgag ttcagcgaca aattgcttcg gttgcatctg cgaacccgaa tcgaattggc
     9541 gctgctccgc gccttcaatc atcagcttga cgtgcgtgcc gccgatatct accacgagaa
     9601 ttttctggag catgaagcga tcgctaagat cgataatgat cttattcgcc catgccgatg
     9661 gaacgcgtca ggcgctgaaa gatgccggtt ttctccggct tcacggcggc ggttgctggc
     9721 caacactcga cgatttcgag cgcgagaaaa cggaaaaggg tgagtcgcgc aaattttagg
     9781 tcgagccgca gatttctcgc gatctgctgg acggaacggc tgccgttaaa ctgcgaggca
     9841 aactgcgctt ctgcgacggt gagcttgagg ttctgggcac gctcaaatcc atctttcgtg
     9901 taggccggaa tgcacgccac ctcaatattt ccgtggtcac ccaagtcctg gaactgaagg
     9961 aaacgcaaac tggccagcgc ccagtggtcg agatccgcct cgcttggcag gtcggttgcg
    10021 tattccggat aatccgagct gcgttcgaac aggaagcgca cgtgcggcgc ggtccacagc
    10081 tgcgcgaaca gtttttgccc ataatgctgc accaattgca cggccggctc gcgcaggatc
    10141 aactcctgtt ccgccagcgt caggaaaagt ggacaaccgc tcaggccctg atcggaccgc
    10201 gcggcaacga tccgctccgg atcgacgttc accagtgtaa tgggctcatc ggggcaataa
    10261 agttgcgcgt cgcgtgtcgt gacaaggacg atctcgccgt tgcgcgtgag cagctcgacc
    10321 ggtgccttgg gccagtacgc tcgcaaccag ccggtcaact tctgcttccg aatcgtttgc
    10381 agcgcgcgat taagcgaaaa aaacctcgtg tcgccgcaga aaaacggacc accggtcact
    10441 gattcgtcgg cgacggcaac ttccgctgct gcagtcggtg ttggctcggg gcgcggcatc
    10501 ggctcgatga cggtcggcgc ggttgaatcc ggcggcggaa tttccgccca caaatcctct
    10561 gaggggagtg gcgccatgtc cgtttcgatc ggcgtcggtg gcgaggcttg acttgaagta
    10621 acctcagcgg gtgtcgcttc tggagttcgc ggcggctctt cggcttgctc ctgaaacaaa
    10681 tccgtcgttt ccggctcggc caattcttcc accgccggag ccgctgagtc ggtgttcgtg
    10741 ggcagatcag ttttcgccgc tgacaggagt tcggtgtccg gtccggcggc ttcggccggc
    10801 tcgacttcgc cgcgagattg ggacgtatgc tcctccactg tctcaacaag caaatccgat
    10861 gtgaatggtt tgttcaagaa tccaatcacg ttgcgcgggt tcgcgccctc gcgtttgaga
    10921 tcggcaccgt agcccgacat ataaaccacc gggacatccg ccgtcgtcgg gtcgtccagg
    10981 agtcgacgac taacctcgtc gcccttcatg tcaggcaaga tgtaatcgag cagaaccaga
    11041 tccggccgct cagccttgca cgcatccagc cctgtccgcg cggctgacgc ggtacgcacc
    11101 tcgtagttcg cctcggccaa aacttcctcg acaaaactga gcagcatcaa gctgtcgtcg
    11161 atcacgagca cttttcgctt taccgattcg gctcgcggtt ttttcttcga ttcaccggcg
    11221 gccgcgtgct catcgctctt ccgcgccgct tccatgagca gaaattgcca gtcctgatcg
    11281 atggttcgtg gcgcgggcgc ggcgccggaa acttccgaga ttgtgccgcc cttccacgac
    11341 acaatttcgt tgaaagcggc aatgccttgc gtacctggcg cggtggcgtg gcgtacctga
    11401 cctttttcga aaaagacgcg cgccttttcg ccccgcggcc cggtgaactc cacgaccgac
    11461 gtggcgccgc tcatgcactt gagctggatg acgtcggcga tgtgcaggtc gctgagcgtg
    11521 ccgcgaaatt tctcggcggc tctgtccggc gcgcgcagca acgcctcggc aagatcgaca
    11581 aaatcacgat gcggaaacgg tttctcgaga aaatgaatcg cgccgagacc ggcggtctgc
    11641 tcgcgttgtg ccggcagatg ggttgcggtg agaatgatgg cacgcgcgtt cgggtgactc
    11701 gtgcgcaagt cgtagagaaa atcctgcccc agatcctgag tcgcgtcgat atcgaggacg
    11761 aaaagatccg gtttggtgcc gacgacgagc tgctgcgctt cctgcaagct gtgggccatg
    11821 tccacttgcg ccgccggcgc cgcttcatgc accgcgctca aaatcgccga ggcgacttgc
    11881 tcctccggtt ccagaaccat gacccggcct tcttttggcg gggcgtcatt tgcagtcatt
    11941 cggttttctc ctcgtgattg ggacagtggt taggatagcg gggcgatagc gctgactctc
    12001 ctttaggcaa tgcccgcttc tcggcaaatg caaagcattt ttcctacagg cggactgtcg
    12061 atgtctaaga tacaaattgc cgcgccaatt ctcgccggct gattttcccc cgctcgttca
    12121 ccggcagttc gtccacaata aagatccggc gcggcacctg ccaggaactc aggtgctggc
    12181 gacaaaattc caggagcgcg tcttcggtga ccgtcgtgtt gcccacgacg cacgctgcga
    12241 cctcctcatt ccgcagcgtc gactcgcggc cgaacgcgat cgcctcccgc acaccggcaa
    12301 agcgccgcaa ctgcgcttcg atttctgccg gatggacttt tttcccagcg acgttgatca
    12361 cgtccgagac ccgcccgacg atgcgaaagc cttgtgcagt ttttgtcagc aaatcgtccg
    12421 gaacaaagac gccgttgcca agtttctcct cattcgcatc ggggaaataa ccgtcgccca
    12481 ccgtggcact gcggacgcgc acctggctga cctgtgcgtt atcatcgatc attgcaacat
    12541 cgacgtgttt catcggctga ccgacaaacc cgtcgatgtc gttggccgct tcgcgatcgt
    12601 agcaaatgcc gccgcattcc gatgcgccgt aaaatgagtg gatcggcaga ctgaatttct
    12661 gctggaactt gcgcgccacc gaaaccgaca acggcgcgcc ggccgaaatg cacagccgca
    12721 gccgtggcaa caccggcgcg tcatcgattt ccgccagcgc ttgatagaac gctggtgtgc
    12781 cggggaatac cgtcgcgttc gttcgcgcaa tgtcggcgag aagcgcccgc ggtgttcgat
    12841 cccgactcag caccagcggg acgccgcgcg cgatcagcgg cgtgagcaaa ttgctgaacc
    12901 cgtaggaatg cgagattgga atcacgccga agttaagatc gacatcttcg atccccatcg
    12961 tctcgcagat ttggtcgcaa tcggccagga gctgcccgct gcggaagcgg acaaggcgcg
    13021 gcctggctgt tgtgcccgag gttaacttga aaagcgacgg cggcttatcg ccccaatcga
    13081 caatgccgct cttcgccgcg attgaaaccg cggtatcagc ttgttctttg ccgacagatt
    13141 cgtcgatcgg aagaacaaca cgctgtgtcc gcaaacacgc cagaaaaatt gacggccaat
    13201 tctcgtgatt tccgatctga attgcattta cgttgtgcgg cttaaccgtc ggaaacttcg
    13261 cttcgtcggc gcgggcgcgt tcctcgattt ggcgaaaggt tcgcacgact tcgccgcgcg
    13321 tgttgaaaat tgctggcgcg tcgcgtttac gcgcgagagt ttcctcccat gcagcaagca
    13381 gcggatcgtc ccatctcatc ggttgcgaat ctgccctaat gagctaggtt tcgcgctccc
    13441 tttttgatac atgaaagcgg gcattcatcc ggaatattac gagaccgata tcgtctgcgc
    13501 ctgcggtgcc gtgtaccaca cgcgctcgac gcgtcgcgat attaagatcg gcatctgtgc
    13561 tcgttgtcat ccttttttca ccggcgaaca gaagttcgtc gacaccgctg ggcgcgtgga
    13621 gaaattcgcc cggcgttacg gcagcaccaa agccgcgcgc ggcggcaaaa aggcttagct
    13681 gacttcttgt agaggcgctt gtgccaagcg cctgggcggc tgatacagcc gcccctacaa
    13741 tgctcatgga ctttgattct ctcattcagc gcaaacgcga gcagttcgaa gaactggagc
    13801 gcgaaatcgc cgatccggcc ctgttttcga atcgcaaacg cgccggcgaa gtgatgcgcg
    13861 agcacgcgag catcaaacag gtgctcgcga aatggaatga gctcgagcag gcgcgcaaag
    13921 agctcgacga cacccgcgag ctggcgttgt cgaacgacgt cgcgatggca gcaatggccg
    13981 aagacgaaat cgtcgagttg gaaacgcggg ttctcgatct tgaacgcgaa ctgcagatcg
    14041 cgttggtccc gcgcgatgaa cacgaagagc gagacgccat cgtcgagatc cgcgccggca
    14101 ccggtggcaa tgaagcggcc attttcgccg ccgatcttta ccgaatgtac aaccgcttcg
    14161 cggaagcgaa cggattgaaa accgaggacc ttgaatcgag cccatccgag ctcggcggac
    14221 tcaaggaagt gatcttcaga gtctcgggcg aatcggtgtt tcaaaagctc cgttacgaaa
    14281 gcggcgtcca ccgcgtgcaa cgtgtgcccg ccacggaggc ccagggccgc attcacacct
    14341 ccaccgctac cgtcgccgtc ctgccggaag cagaagatgt cgatcttgaa ctgaaaccgg
    14401 aagatctgcg gatcgaagtt tcgcgtgccg gcggccctgg cggacaaggc gtgaacacaa
    14461 ccgactccgc cgtgcaggtc ctccatatcc cgaccggcac gatggtgcgg tgtcaggacg
    14521 gccgcagcca gatcaaaaac aaggaacgcg cattgtcgat cctgcgcgcg cgtctactcg
    14581 aacgaaaaca gcgggaggaa gccgagaaat acagcgcgca acgccgcggc cagatcggca
    14641 ccggcggccg cgaagaaaag atccgcacct acaactttcc ccagaaccgc gtcaccgacc
    14701 atcgcatcgg tctcaccctt tacaatctcg accgcattat ggacggcgac ctgaacgagc
    14761 taaccactgc gctccagacc gccgacatgg gcgaacggtt gaaagactcg tcggtggcat
    14821 gaccctgctg gaggtgctgc aaaagagcac cgattttttg gcgaagcgca aggtagagaa
    14881 tgcgcgactc aacgcggagc acttgctcgg gcatgtgctc ggacggacgc ggatggagct
    14941 ttatctcgag ttcgagcgcg agctcaatga acacgagtta gcgccgcttc gggatttact
    15001 acgtcggcgt ggcgaacgcg aaccgctgca gtatttgtta ggcacggtcg agttttgcgg
    15061 agagacgttt ctatgcgaca agcgcgcgat ggtgccacgg ccggagacag aggaactcgt
    15121 cgagttcctc aggtccgaaa tccgaaatcc gaaatccgaa atacttgacc tcggaacagg
    15181 cagtggagtt atcgcgctga gtctcgcgaa acaatttccg gaagcggagg tcgtcgcggc
    15241 agacatgtcc gatgaagcgc tcgcactggc gcgggagaac gccgagcgac ttggactcag
    15301 cgagcgtgtg cagtttctga aaagcgattt gctggcaaat gtcttcggca catatgagct
    15361 catcgtggcg aatcttccat atgtcgccac gcaagatcga caatcactct cgcgcgaggt
    15421 gctgcacgat cccgagatcg cgctcttcgg cggcgagcgg ggggacaact tgattcggca
    15481 gctgatcgat caggcgccgc tgcacctgaa accgggaggg ttactcgccc ttgagatcgg
    15541 catcggtcat gctgcatcgc tttcccaggt tctggcggaa aaaaaatacc acgacattgc
    15601 ggccaaaaac gattattcag gtgtgacccg ttttctcctc gcgaggtatg gataaaatcc
    15661 gcatccaagg cggtcaggcc ctctccgggt cgatcaagat cagcggatca aaaaattctg
    15721 cgctgccgat tttggcagcg acgttactga cgcgcgaaca gtgcatcatc catcgcgtgc
    15781 cagacctgag cgacacgcat tacatgctgc aaatcctgac ccatctcggc gcacaggtgg
    15841 agcgcgcgag cggcattgtt tgcgtcacgg cggaaaaggt gaactccgtc gcgccctacg
    15901 acgttgtgcg caaaatgcgc gcttctatat gcgtgctcgg gccgctgctt ggccggtgca
    15961 acgaagccac tgtctcgatg ccaggcggct gcgtgattgg ggaccggccg atcgatttgc
    16021 atctgaaggg attcgaagcg ctcggcgcgg ccgtgcgcgt ggagggcggc aacatcaagg
    16081 tgtttgcgcc gaagttgcgc ggtgccgtca ttaacatggt cggtcggttt ggtccgagtg
    16141 tgctcggaac cgataacgcc atgatggcgg cagtgcttgc ggaaggcacc acggtgatcg
    16201 acggcgcggc gcaggaaccg gaggttgtcg atcttgcaaa ttttctgatc cagatgggcg
    16261 ccagaattga aggggcgggc acacgccgcc tgatcatcga gggtgtaccg gagttgcacg
    16321 gcgccgagta cgagatcatt cccgaccgaa tcgaagcagg cacatttctg gttgccggcg
    16381 caatctccgg caagcgcgtg acagtcaaac gtgtcgagcc ggagcatgtc cgcgcagtca
    16441 ccgacgcatt gcgtcgctgt ggtttcgcca ttgaaacggc cgacgatgcg atatcgattt
    16501 gtccaaacgg cgcggcccaa ccactcgagt tcagcacgga gccttacccg ggctttccca
    16561 ccgatatgca ggcgcagatg tgtgcgttat tgtcgacaac cgacggccga agcgtggttc
    16621 gggaaaatat ttttccgcag cgctacatgc atgtggcgga gctcaagcgc atgggggcgc
    16681 agatggaact cgatggtgcg acggccttga ttaacggtgt cgatcatctc tacggtgcac
    16741 cggtgatggc gagcgacttg cgcgcatcgg ccgcgctcgt cctcgccgga ttgaaagccg
    16801 atggtgtgac agaagtcagc cgcgtttatc acatcgaccg gggctacgaa catctcgatg
    16861 aaaaactgcg cgagctcggc gcggacatcg agcgagtgaa agggtaagcg atgaacgaaa
    16921 cgccgccgcc gcttcctgca acatcggcat catccgtgcg cacgtggtgc gtgctggcac
    16981 atgccctcgc gctcatcggc ttctttgtcc catgggctgg ccatattatc ggcccgctca
    17041 tcgtctggtt ggccaaacgc ggcgattcac cggagatcga ggcgcacgcg agagagtctc
    17101 tgaactttca actctcgatg ttgatctaca atgcggtcgc ggcgctgtta tgcctggtgc
    17161 tcatcggttt gctcatcctg ccgatcctgc acatcatcaa tgtcatcctg gtgatcgtcg
    17221 cgtcgattcg cgccagcgaa ggtaaactct accattatcc gctcacgatc cggctgatca
    17281 gctaacgtgg cggttttatt tcaattagtc cgcgcaaggt cttcaagttc tcaatgtctt
    17341 ggcgcagatc cttttccttt ggggtctcga gcactttcgg aattttagcg aatcggcagt
    17401 cgcggaggat cgcgcgaaag gcgttgaggc cgattttgcc tttgccgatg tgttcatgtc
    17461 gatcgacgcg cgaaccgcgt ggtgccttgg aatcgttgac gtgaatggcg gcgagccgat
    17521 taagtccgat cgtgcggtcg aattcgcgaa acatttttcg tactgccggc tcacttgaaa
    17581 tgtcgtaacc ggccgcgaac acgtgcgccg tgtcgagaca aacgcaaagc cgctccggct
    17641 ggcggacatt gtcgatgacg taggcgattt gctcgaaagt gtgaccaaga caggagccct
    17701 ggccggcggt cgtttccaat gcaatttttg ttttgacctt cggcaggaca cggaacgcgg
    17761 cgtcaatcga cgcgatgatt ttctcgagtc cggcttcttc gccggcgcca aggtgtgcgc
    17821 ctggatgcaa aacaagaaaa ggcaactcga gttggttcga gcgcgtcagc tcttcggcca
    17881 aagcgcgaag ggaattcgca tgaaactgcg gattcgtcgc cgcgagatta atcagataat
    17941 tggcgtgcgc gaagatcgac aacagttcgc cgcgctgcac gtgattgcag aaagcgcgga
    18001 tctcctcgcg cgtgagtggg cgggcgaacc attgcatgtt gttcttcacg aacatctgca
    18061 tcgccgtgca ctggatggag caggcccgct cgatcgccat gtgcacaccg ccagcgatcg
    18121 acatgtgagc accaaggaga atttgttttg tcatgtcgag cgaagtcgag acatctctca
    18181 tccttaaaca ttaagagatt cctcgacttc gctcggaatg acacaggcta aaatctcttg
    18241 gggtgccagt ccgggtgctt gagactgcct tcgccgacgt attcgaaaag tttgtagacc
    18301 ggcgtgagca aaatactcgg gcccttgccg ccgatacgaa ggtaaaaatc gagtttgtcg
    18361 tcgaggaagt gaatgtcgcc atggccgagt aagcggaaca atcgcccggt cgcatcgaag
    18421 tcatcggtgt gaatgattcc gtctttaatg gtgaatctgg cgtcagcgcg gtgcgcgatg
    18481 ctgtacccag ccccgggaat gatgctgttg aggatggctg agagcgggcc aaagacggga
    18541 atggcaaaaa catcgccgtt cgtgacctcg agtgtaccgc tgccttgcat cgcccgcgca
    18601 gccgtgccga ggccggtgaa atcgtaggtc ccgctgagcg cgccctgcgc ggtcttgtag
    18661 ttgtagtaga gatcggtcag ctgcgggaaa ttgatttcag ccaccgcaat cttggcgtgg
    18721 taatgcggat catttttcgc aagcgagatg tcggccgtgc cgtggacgct gccggagaac
    18781 aacccggcct taagatcgac aatttgcagg cggtcattcg tgaacaggag gcgactgacg
    18841 acgcggtcga acggcaacgt tttgccaaga aagccgtagt ccattccgcc aggtgcttcg
    18901 acgccgatgt cgatgtgcgt gttcttgccg ccgcggaact gatacacccc atttgtcgtg
    18961 acgtttggcg ggcggcggaa cttatatggg acgacggttt tccagagttt cgggtcaacc
    19021 caaagaatcg cttcagccgg acgcagcgac gttttgattt tgttgatgcg gacttcgtga
    19081 tttttgaaat cataagcgaa gcttccggtg ccgatgcctt cgtcgcgcgt cacccgcaga
    19141 ttgtcgtaca gaatcgcacc gtcgccgaag cgcacattcg ccgtggccga gttcatccac
    19201 gcgccgcgga atcgcgcccg ctcgagcgcg attgtgccct cgcctttcca gttttccggc
    19261 tgtcgatcct gcgagtggat cacgagatga acatcgggcg gtcgcggcca ttcccattcg
    19321 ctcagaaatt gactcaactc cggcgagacg agcggacgta aaattgccgg actcagatga
    19381 ctgtcgattt tcagccggaa atcgttcggc gcatcgaaca actgcgcgct cagatcacca
    19441 gtttgatgtc gcaggcgcac atcgcgcaac aacgtgcgct cgccatccca ggagaattgc
    19501 ccgcgtaagt cggcaaatgg cacgcctttg tagctgaaag cgccgacact tgcgcgaccg
    19561 atcactttga tctgcggacg ctcgccgcca aaacttgcta cgcctgagag ctcgagcgcc
    19621 gggggcgccg taaatgcagc atcacgcagc ggctgaccaa ggccgaatgc atccagaaaa
    19681 tctttcagag cgagatcgct ccggatttga aaattcgctt cattcgtccg gcggccccag
    19741 ctagccttgg cagcgaacgt tccgacaata tcccgccact ggcagcggct gatgttgaac
    19801 acctgatccg tccattccgc actcgcgaat agctcgctga ttgtgtaagc gccccggcgc
    19861 agctcctcac cctggagcgt cgcttcgagg tgcgcgtctt cgagttgttc caggtccccg
    19921 ctaaacttca cctgcagcga cggacgagca cgcgggaaat taaacttctg cagctcaatc
    19981 gcgacgcgtt gcaacatcga caatcgcatg cgccattctt cctccgtcag tgccgaggac
    20041 ggctgataac tcgtgcgctt gatcaattgc cccgtggctg agatgcggag cccgcaaaat
    20101 attccttcag cctgactgac ataaatttgc tcgggcggaa aatagatatg cgcgcgaaag
    20161 ttcgtcagct gcgcctgcgc ggaacccgtg tcgctggtct tgatgggaag cgtgatttgc
    20221 gcgttgcgaa tgtcgagtgc gttgagaaac ggttggtgat gcaaaagggc cgcgtaattg
    20281 atgtcgagcg caatctcgct gatacgcgcg agcgtgtttt cgcgccgctt gtaatcaaaa
    20341 atgcgcacgt ccttcgcaat cagcccgcgg aacgggtcga gtgtgagccg gcggatgtgg
    20401 gcttcgacgc cgtgtttgtg cagttcttca gcgatgcgag aacgccattc gcgaccgaac
    20461 ccctttttgg aaaggtacca acctccggtg ataaaggcgg agagcgccag cacaacggcg
    20521 agccgaacgg cgctgcggac gaagcgacgc gccaagctcg gttgccgatc ttgcggccgg
    20581 cgtcgcgtaa cttccggcgg ggtttcgaga acggcgggca tggtgattcg ttgaaacgtt
    20641 gaagcgttaa agggttacat tgggcaaccg gttcaacgct tcaacccttc aacgctacaa
    20701 cgtgatttca atctccgatc tgccggcaat caacgcttcg ctcaatctgc tgagcacgat
    20761 ctttatctcg gcgggctggt tctggattcg acgaaacatc tggcagcgac atgtcccatg
    20821 tatgattgcg gcaattatta catcgacatg tttcctcgtc agctacgtcg tgtaccacgc
    20881 ccacgctggc gagaaatcgt cgggttacac cggctggctg gcggcgattt atttgcccat
    20941 cctcgcctcg cacgtattgc tcgcgttcat cactctgccg ctggtcgtcg tgacgctggt
    21001 gccggtgttt caacgtcgct gggaccgcca ccggcggctc ggtcgctgga cgatgccgat
    21061 ctggctgtat gtgtcggtca ccggtgtgct ggtttatctc atcctctacc actggttccc
    21121 cccggccaat ctgaaatagc ggtcgcgcac cgcgacagag ccctggtccg gcgacggagg
    21181 acggcagcca ctaccgtccc gccaaatctt gcgctcgctc gctaattgat gtttgatcag
    21241 ttccgcgcca catgaggcaa acggcaccat ggtgacgatc gcgacattca acgagccggc
    21301 caaggccagg cagatcaagc agcgttttca gcaggctggt gtgcgcgccg acatccataa
    21361 tgaagcgccg ttgcagcagg tcgggttcat gtcgcggccg caggcaaacg ccaaggtgat
    21421 ggtggatgac gacgagttcg agcgtgcgca gactctcatg atcgagtggg aggcgaacga
    21481 tgcggaaatc gccgccgcga ttgttcgctg cccgcagtgc gggtcgcccc agatcgagta
    21541 tccgcagatg acgcgcaagt tcatcacgcc ggcaatggtc ggcctgcttt gtgcgctcag
    21601 gattatcccg aaggaatttt actgtcagga ttgccatttc acctggagca acgaagaaga
    21661 gcgcaccatc gggcggctgt ggcaccgatt ctttcccggc gccgagcaaa caagctgatt
    21721 cgcggccgca caccgcggct ggaaaagcgc gccgggacga atgatacatt tagcattcgt
    21781 ttcgcgcgct catgaagtgg ctcatcgctc tgctcctcgc gttgcttatc ttcggcggcg
    21841 catggatcgg gtacaatgtc gtgttccacg aagacattca ggtgcgcaaa gaagagcgcg
    21901 gcgaggtcgc gcctgccccg acacccgaca tcagtctgcc ggaatttcag gcggccgcga
    21961 aattgcgtca ggatggcaaa ctcattgaag cgcgcgacgc gctgagcgcg ttcattctca
    22021 aatacccgag cggcctgcac accgaagagg cgaaggatct tctcggcgaa atcaatgtcg
    22081 acatcctgct ctcgcgcatc ccgtctccag aaaaggaaga atacatcgtt aaaccgggcg
    22141 acgtgctggc gaaaatctca cacaaactga agaccacgcc tgagttgatc atgcgcatga
    22201 acaacatgag cagcacgatg ctgcacattg gcgagcggct gctcatttcg catccggatt
    22261 tgagcatggt cattgaacgc aaaggcaaac tggtggtgct gctcaatcac ggcgcgtttt
    22321 tcaagcagta ccatgtgctc gaggagaaat tgtcacccag gcagccgccg aaggtgaata
    22381 caaaggtcgc cgagatcatg gcctggcgtg acggcaggcg cgtaggtttc ggcacgaaag
    22441 aattcatcgg cagcacgcgc tggatccggc tcggaacacc ggcgtatacc atctattcgg
    22501 tgccggactc ggcccatcca aatgtcgggc aacccccgcc ggctcaaggc atcggtctgg
    22561 ccgcgacgga tgtggaggag ctgagcggac ttgtaaacaa ccgcacccct gtcacgatta
    22621 cggattaagc ttgtaattcc tggaaacgtg aaacgtgaat cggccaccac ttgactcgtc
    22681 cgcgatgtaa cgatttaacg aatcacgatt cacgcttaca tggacctcca gccgctcgac
    22741 tttgaaaaac cgctctttga gctggaacgc cggttgcgcg atttgaaaac gcattccgac
    22801 gagcacgatg tcgatcttga tgaagcggta aaagcgatcg agacaaagat caccgaaacg
    22861 cgcgggcaaa tttatggaaa gctgacggct tggcagcgtg tccagatcgc acgacacgtg
    22921 caacggccct tcgccctgga ttacgttgag cgctgtttca cgaactgggt ggagcttcac
    22981 ggcgaccgcc gtttcgccga tgacaaagcg atgccgtgcg gaatggccat gttcggcacg
    23041 cagcgctgtg tcgtgctcac gcaccagaaa ggccgcaata cgaaagaaaa cgtcatgcgc
    23101 aatttcggct gcgcgcaccc ggaaggctac cgcaaagcgc tgcgcctgat gcggctggcg
    23161 gaaaaattcg gcatgccggt gatctcattg atcgacacac cgggcgcgtt tcctggcatc
    23221 ggctcggagg aacgccacat ctcggaggcg atcgcggtga atttgcgcga gatgatgaac
    23281 ctgcgtgtcc ccatcatcgc ggccgtgatt ggcgaaggcg gctcaggtgg cgcgcttggc
    23341 atcggcgtgg ctgatcgcgt cttgattttc gagaacgcgt attattccgt catcagcccg
    23401 gaggcgtgtt cggccattct ctggcgggac cggcgccacg ccgctgaggc cgcagaagcg
    23461 ctcaaactca ccgctcacga cttgctcaac ctcggagttg tcgatgaaat cgttcccgag
    23521 ccggaaggcg gtgcgcatca cgattacaac gtggcggccg ccaatctcgg cgcagcctta
    23581 cggaaaaacc tcgaaggcgt ccgcgcgctg gtgatcgacg aactgatcga aaagcgctac
    23641 gacaaatttc gcgagctcgg caacttcgaa gaagcaccaa gcgcggcgac agctgcgcgc
    23701 gcctgaatgg tgctcggcgg caattgcgag cgcgtgatcg ctccgctcgt gctccttgtc
    23761 gtggcgcatt tgccgaatgc cgacgccgcg ccagacaaat tcagttacgc gcgcgacacc
    23821 ttcgccttcg ccaacgcgac ggtgtttaag tactatcgcg ggattgcgtt tttgcggtcg
    23881 ccgtcagaaa gcgaccgcaa gctctacacc cggcgctgtt tcgtcatgac gcgcggcgcc
    23941 gtgcaatttt acaagtttgc gcggttcgat ccaaatggcc agccgctcga cgatcgcgaa
    24001 ctggccagac gcattcgcgc cgtgtcgcgg cgagcgccat ggcacgcgcc actgccagat
    24061 agtcagcgcg tagtttttcc cggttacgca aacctcaacg acatgagcaa agcccgaacg
    24121 aaggtggtcc agcgaaacat cggcctcggc tggcctactt atatgcgcct cggcaatttc
    24181 cgcatgcttt tccagcacga tccgcgctat caggaaaaga cgcacaccaa cctcaacgcc
    24241 gcgcttgacc ggggcgattt gttcatcggc tatctgtcca cgtttccgag tctcgcgatc
    24301 aaccacagca tcctcgttta cgcgcgaaaa ccagagcggc ccgaccacga cgttgaacaa
    24361 cattatctcg cttacgaccc gaatcacccg gacggcccgc gcgtgctaac gtggtcggcc
    24421 aggaaccgcg cctttgctta ccaaaaggat tccgatttca tcggcggttt cgtccgcgta
    24481 taccaaatct acggtaagct gctgcaataa tgccgaaaaa accgccacct tccgagccac
    24541 cgcccgcccc cgcaccgccg cccgagccgt tgccgccgaa cccgccgccc attccgccgg
    24601 ttgcgcgctc aatggcacgg gcgtcctgcc cgtgatttct gcaattcatc ggctggaagc
    24661 cgatgccaca ttatcttcgc cggagcggcg gatataaaaa gcgcagcgct ggatcgacgc
    24721 gctcggccca ggccgcctca ctgtgctcac cgcctctgcg ctcgctgtat tgcagatcac
    24781 ggtgcaaaat ccagcctttc tccaccaaag catcgcgcaa gtgccgcacg cgttcccaag
    24841 cttcttcctg cgtgccggta tccagccaga ttttatgcgg caatttgcga tcgaggtcgt
    24901 ccaccatttg cacgatcacc tcgttgtccc accaaaccga gggggacatc gcgatcagtt
    24961 tcgtgaaaat gttcggaaac cacaggccta acgaaagtgc gagcaaaccg cctaaggaag
    25021 agccgccgat cccggtaaac tcagcgtcgc gtctggtgcg gtacttccgg tcgatgtacg
    25081 gcttcaactc gccaatcagg aactcgccat attttcggcc gagacctttg ctgcgtttct
    25141 tgcgcgccgc tttttcgctg agcaccccgc gggtcggcgc gtactcgtga acgcgatcct
    25201 cgcccatgtt cgcgacggcc acaatgatta gcggctcgat cagatttttg cggataaggc
    25261 gctcggccgt ttcgtcgacc ctccattcca ctccaccgaa cgccgtcgca gcgtcgaata
    25321 cgttttgccc atcttgcagg tacaagactg gataacgcgt acgtgaaaaa cgccggtagc
    25381 ccggcggcaa gtacacgagc acatcgcgcc gattgcccag aatccgcgag gcgaatccgc
    25441 gatggcgttt gatgtttccg gtgtgtgtgt gttcggccat gaggaaggac gcatcagtga
    25501 agatgtcgat cttgtccacg gggaatcgcc tgattgaccc tgtttaggcg ctctcgttat
    25561 tgtcgatctt cccacgatgc acgaacgcta catcaaatgg tacacgcaat ggctcagccg
    25621 cgactttgag atgctcatct tcggccacgg cggcggactg cctctcattt tgttcccgac
    25681 ctctttcggc cgctattatc agaccaaaga cttcggcctg gtcgattcgg tcgcgccctt
    25741 cgtcgattca ggtcgcgtga ccgtttactg cccggacgca attgatctcg agagcttcta
    25801 caacaaatca attcatcccg cggatcgcat gcgcacgcac aacgcctacg agcgcgtgat
    25861 tgttaacgac gttttcgagt ttgcgcgacg cgaatgttcg gcacaccgcg tagccatgag
    25921 cggcgcgagt ctcggcgcat accacgcggc caatatcgca tttcgttatc ccgacctcgt
    25981 cggcgcgctc atcagtctga gtggttcgtt cgacatcagg tcgtttttcg acggctacca
    26041 cgacgacaac atctacttca atagccccta cgaatatctg ccgaacatgc ccgacccgtg
    26101 gaaatacaac cacatgcgca tcatcatcgg cacgggcgaa tgggacaaca cccggcacga
    26161 atcgctacgg ctcgccgaga ttttgaattc caaaggcatc ccccactggc tcgacgaccg
    26221 caaatggtgc ggccacgagt ggagctactg gcgcgacatg ctgccctact atttgtcgac
    26281 gctgtgaact cttcctgtca tgtcgagc
//
LOCUS       DASTDG010000129         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_87955, whole genome shotgun sequence.
ACCESSION   DASTDG010000129 DASTDG010000000
VERSION     DASTDG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87955"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFO30_04205"
     CDS             complement(<1..1005)
                     /locus_tag="VFO30_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418522.1"
                     /translation="MPTALLIDDDVESRKSTAGLFVRRGWEVIEADQGRRGIELAFEH
                     QPTLIVCDLLLPKLSGLQVCRAIRERLDSAKAIITAGRHYDVDREAVLEAGVDDYFVK
                     PLTWQRIARVLKRRARRRRKLPPKHSPAFKFEPPRTRLKFWGVRGSIPVPGPSTIGYG
                     GNTTCVELRTDSEIIVLDAGSGIRELGLTLDQEFGTAPIKLTLLLTHTHWDHIQGLPF
                     FRPAYNRKNSIRVLGYEGARAGLASILAAQMEIPFFPVSWRDLPSTIQVEELKTMEFS
                     IGKVRVRAKILNHPGVCAGYRLFTREGSIAFLPDNEPFEPLKLKLAARDGIPPEKARA
                     Q"
     gene            complement(1118..1522)
                     /locus_tag="VFO30_04210"
     CDS             complement(1118..1522)
                     /locus_tag="VFO30_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418523.1"
                     /translation="MAIDPSKPAGDFEIVASERSGETRSLAKPDPAATPFKVVIDPKV
                     RQALRRALIDLIEFHDEALAEHFAEGKLALDSYKEYIELFARSLKETMESREGVAYLL
                     RSAGFDVPADEINLQPDLRWKKGPAPFGSTLI"
     gene            complement(1609..2148)
                     /locus_tag="VFO30_04215"
     CDS             complement(1609..2148)
                     /locus_tag="VFO30_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9418524.1"
                     /translation="MARAAFELKNTPSFCRLIDVRRNNRLYPRRPPRLGWLFQNVQPF
                     YFVTFNTHNRLPMLATPEVHEAFELFCRRAQERDIFVGRYVLMPDHVHLFVSLPSQGT
                     TLAQWVQALRSILGKTLLGLGVAKPHWQDGFFDHVLRNSESYAQKWEYVRMNPVRAGL
                     GKTPDEWPYQGEIARLSFD"
     gene            complement(2227..>2617)
                     /locus_tag="VFO30_04220"
     CDS             complement(2227..>2617)
                     /locus_tag="VFO30_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET9418525.1"
                     /translation="VPEYGTCVDIVTKAALREMIVPALLPLIFVITVAVIKPLGPVVL
                     GGLLVGTIVTGLFVAIAMTSGGGAWDNAKKFIEEGNLGGKGSFAHAAAVTGDTVGDPY
                     KDTAGPAINPMIKVVNIVAILIIPIFF"
BASE COUNT          552 a          781 c          739 g          545 t
ORIGIN      
        1 ctgcgcgcgg gccttctcgg ggggtatgcc atctcgcgcc gccaatttca gcttcaacgg
       61 ctcgaaaggt tcattgtccg gcaaaaacgc gatcgaacct tctctcgtga aaaggcgata
      121 gccagcgcag acgccggggt gattcagaat ctttgcccga acccgcactt tcccgatgga
      181 aaattccatc gtcttgagct cttcaacttg gatggtgctt ggcagatcgc gccagctgac
      241 cgggaaaaat gggatctcca tttgcgcggc cagaatgctg gcaagcccgg cgcgcgcgcc
      301 ctcatatccc agcacgcgga tggaattttt gcgattgtag gccggccgga aaaacggcag
      361 cccctggata tggtcccaat gggtgtgggt aagtagcaac gtcagtttga tcggcgctgt
      421 gccgaattct tgatccaagg tgagtccgag ctcgcgaatg ccagagccag cgtccagcac
      481 aataatctca ctgtcggtgc gcagttcgac gcaggtggtg tttccgccgt agccaattgt
      541 cgatggccca gggacgggaa tcgatccgcg caccccccaa aatttcaaac gcgttctcgg
      601 cggctcaaat ttgaaggccg gcgaatgttt cggcggcaat tttcggcgtc ggcgtgcgcg
      661 gcgtttcaac accctggcga ttctttgcca ggttaacggc ttgacgaagt aatcgtccac
      721 gcccgcttcc aacaccgctt cgcgatcgac gtcgtaatgt cgtcccgcgg tgatgatggc
      781 cttggcggaa tcgagtctct ctcgaatcgc ccggcagact tggagtccgc ttagcttcgg
      841 cagcaagaga tcgcaaacga taagcgtggg ctgatgctca aacgccagtt cgatcccgcg
      901 cctgccctga tcggcttcga tcacctccca gcctcggcgg acgaacagcc ccgccgtgct
      961 cttgcggctt tccacatcgt catcgatgag caatgcggtc ggcatggtgc ggatttttgc
     1021 acgtcaggtg caacgaaacc aaccgttttt gaaagcagca ccgtcgccac ggcgccattg
     1081 ccgtgcagcg ggcttgggct aatgccgtgt cgctgcgcta aatcagagtc gacccaaacg
     1141 gggccggacc ttttttccag cgcaggtccg gctgcaagtt aatctcatcg gcaggcacat
     1201 cgaacccagc cgaccgcagc agataagcaa cgccttcacg actctccatc gtctccttca
     1261 agctgcgcgc gaagagctcg atgtattctt tgtaggaatc gagcgcgagt ttgccttcgg
     1321 cgaaatgttc cgccagcgct tcatcgtgaa attcaatcaa atcaatcagc gcgcgccgca
     1381 gcgcctggcg cactttggga tcaatgacaa ctttgaacgg cgtcgcggcc ggatcgggtt
     1441 tggccaggct acgcgtctcg ccgctcctct cggaagcgac aatctcgaaa tcaccggccg
     1501 gtttggatgg atcgattgcc atcaattgtc gatcttacac gcacgagcgc atctttgcgc
     1561 gacaaaattt ttgtagccac ggcgctctgt cgccgtgcat tgcagcagtt aatcgaagga
     1621 cagacgcgca atttcaccct gataaggcca ctcgtcgggc gttttgccca acccggcgcg
     1681 gaccggattc attcgaacgt actcccattt ttgcgcgtaa ctctcgctat tgcgcagaac
     1741 atgatcgaag aaaccgtcct gccagtgcgg ctttgccacg cccagcccaa gcagtgtttt
     1801 gccgagaatc gatcgcaacg cctgaaccca ctgcgcaagc gtcgtgccct gtgaagggag
     1861 cgagacaaac agatgaacgt ggtctggcat cagcacgtaa cggcccacga aaatgtcgcg
     1921 ctcctgcgcg cggcgacaaa atagctcaaa tgcctcatgg acctccggcg tggcgagcat
     1981 cggcaggcga ttgtgcgtgt tgaatgtgac gaaataaaac ggctgcacat tttgaaacag
     2041 ccatcccaga cgcggtggtc gtcgcggata cagccggttg ttcctgcgca catcaatgag
     2101 tctgcagaaa cttggcgtgt tcttcaattc gaatgcggct ctagccacga cgcggcgcct
     2161 gcactgagcg aagtcgaacg tgtcgctacg aatggcaacg cctcggcaga gcgaggcggc
     2221 tacaattcag aagaatatcg ggatgatcag gatcgcgacg atgttcacta ccttgatcat
     2281 cggattaatt gccggcccgg ccgtgtcttt gtaagggtcg ccgaccgtgt cgccagtcac
     2341 cgcggcggcg tgcgcgaatg agcctttgcc gccgaggttg ccttcctcga tgaatttctt
     2401 ggcgttgtcc cacgctccgc cgcccgaggt catcgcgatc gcgacaaaca gtccagtcac
     2461 aattgtcccg acaagcagac cgccgagcac gaccgggcca agcggtttga tgacggcgac
     2521 ggtaatgaca aaaatgagcg gcagcaatgc cgggacaatc atctcgcgca acgccgcttt
     2581 cgttacgata tcgacgcacg tgccgtattc gggcacg
//
LOCUS       DASTDG010000130         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_244907, whole genome shotgun sequence.
ACCESSION   DASTDG010000130 DASTDG010000000
VERSION     DASTDG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244907"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFO30_04225"
     CDS             complement(<1..336)
                     /locus_tag="VFO30_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979677.1"
                     /note="catalyzes the formation of biotin from
                     dethiobiotin; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HET9418526.1"
                     /translation="MKFAELSSLYNQPLFDLISRSRGVHLEYWRGEAMQRCSLLSIKT
                     GGCSEDCAYCAQSAHYSTGIAREELLSIQQVVDVAMRARANGATRFCMGAAWRGVRDG
                     TEKFERVLDI"
     gene            complement(488..1195)
                     /locus_tag="VFO30_04230"
     CDS             complement(488..1195)
                     /locus_tag="VFO30_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418527.1"
                     /translation="MKRVLLICALSIFTVAAHATAGPTPGEWIIVVGGPSLKQWEQYK
                     VYPHDHWWANFVHAARLRTEQLRANLGPDAPITWLVYKQGYVDRGKQEQQDLISHIES
                     VRDKFNLRLVWFKNGSEVIDYLNTSQDRRTFKVAGFEYFGHSNKACFMFDYSNNLDSA
                     SKSWLHESELWKINRQIFAHGAYVQSWGCHTGESMSKKWYNATGTKMVGAIGKTQFMM
                     EELPVLTSPGGRWVTQN"
     gene            complement(1335..2708)
                     /locus_tag="VFO30_04235"
     CDS             complement(1335..2708)
                     /locus_tag="VFO30_04235"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9418528.1"
                     /translation="MHAFRYREGKLYCEDVDLARVATEFGTPVYVYSAGTILDHYTRL
                     DAALAPIDHLICYAVKANSNRAVLKLLADAGAGFDIVSGGELFRVLNAGGAPDKCTFA
                     GVGKSREEIDYALEQRVYSFNVESETELEYIDKIAADKNVRAPVALRANPDVDPHTHE
                     YIATGSHENKFGIPLDQLGAVYERAARMRKIDIVGVQMHIGSQITEAEPFAAAIRKLA
                     PLVGELKSKYAIQFFSIGGGLGIVYESSFESGAKEWWDERRRPSSQCSPSGRGGREAP
                     GGGADASRTHALSIKEYVDAILPTLRELGVRILLEPGRLLVGNAGVLLTRVRYIKQTG
                     RKKFAIVDAGMNDLIRPALYHSYHEIVPVEEPITSTSMSKSTKEMIDIVGPVCESGDF
                     FALDRAMPPLREGDLLAIMSAGAYGFVMASNYNSRPLPAEALVDGDKFKLIRKRQRWE
                     DLVHGEA"
     gene            complement(2755..3555)
                     /locus_tag="VFO30_04240"
     CDS             complement(2755..3555)
                     /locus_tag="VFO30_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9418529.1"
                     /translation="MLWPIAACVLLPWLLVYLGLHVVQRGIIFIDIAMAQMASLGICL
                     AVLLHIDLESWTTFSIALAFTLLGAAIFSVTGKRTSKIPQEAVIGIAYVVAAAAAVLL
                     LSRAAEGDEQIKNMLIGNILLVTPREVWERFALFALVGAFHFAQRKNFLLVSFDRDGA
                     YAKGLRVRWWDFLFYAVFGLAVTSFVRLAGVLLVFSYLIVPAVCGINLADRTGNRLLI
                     GWIVALIGGIGGLFLSYWWDLPSGAAIVCTFGVLLVLIAFIGLWRRRR"
     gene            complement(3622..>4015)
                     /locus_tag="VFO30_04245"
     CDS             complement(3622..>4015)
                     /locus_tag="VFO30_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET9418530.1"
                     /translation="KKFEATINAKLQEWGAAMLPFKGQHLAAYHDSWPYFGHRFGLNI
                     DIFLEPKPGIPPSPSHLTEVIAQMKAQQVKAVIVEPYHDRRIAEKVASATGAKVVDFC
                     QFPGGLPGTDTYVKLIDVLVSRLAAALK"
BASE COUNT          839 a         1164 c         1180 g          832 t
ORIGIN      
        1 gatgtcgagc acgcgctcga acttctctgt cccgtcgcgt acgccgcgcc aggccgcgcc
       61 catacagaag cgcgtcgcgc cgttcgcgcg cgcccgcatt gccacatcga caacttgctg
      121 gatcgacaat aactcttcgc gtgcgattcc ggtcgagtaa tgcgcgctct gcgcgcagta
      181 ggcgcaatct tcgctgcacc caccggtttt gatgctgagc aaactgcagc gctgcatcgc
      241 ttcaccgcgc cagtactcga gatgaacgcc acggcttcgc gagatcaggt caaacagcgg
      301 ctgattataa agcgaggata attcggcaaa cttcatgacg cgcggacgaa ctgacgtgat
      361 ccgaaatgac ggtgagagat tcctcgactt cgctcgggat gacaagagcg gcgatgctca
      421 atcgcaaatc ggcaactccg acggtagtgg cgagcacgcg aacggtttcg gtgtcggcaa
      481 tcgaaaatta attctgtgtc acccagcgcc caccgggtga ggtcaggacg ggcaattctt
      541 ccatcatgaa ttgggtcttg ccgatggcgc cgaccatttt cgtgccggtg gcgttgtacc
      601 acttcttgga catgctctcg ccggtgtgac aaccccagct ctgcacataa gcgccgtgcg
      661 cgaagatctg gcgattgatt ttccagagct cgctttcgtg cagccatgat ttggatgcgc
      721 tgtcgagatt gttgctgtag tcgaacatga aacaggcctt gtttgagtgg ccgaagtatt
      781 cgaacccggc gactttgaag gtgcggcggt cttggctggt gttgaggtaa tcgatcacct
      841 cggagccgtt tttgaaccag acgaggcgga gattaaattt gtcgcgcacc gattcgatgt
      901 ggctgatgag gtcctgctgc tcctgcttcc cgcgatcgac gtagccctgc ttgtaaacca
      961 gccaggtgat cggcgcgtcg gggccgaggt tagcgcgcaa ctgttcggtg cggagccgcg
     1021 ccgcgtggac gaaattggcc caccagtggt cgtgcggata aaccttgtat tgctcccatt
     1081 gtttcaacga cgggccgccc accacaatta tccattcgcc cggcgtcgga cccgccgttg
     1141 cgtgcgccgc caccgtgaag atcgacaatg cgcaaatgag gagaaccctt ttcatgccca
     1201 gctgggggag agattattta tgcacgcggc gaaactgcgc aagtgggcgg atcggcttct
     1261 tcgaaagccc atgttattcg accgccgcgc agcggcgaat gattttacca tcgcgctcgg
     1321 agatcgcgat ccacctaggc ctcgccgtgc accaaatctt cccacctctg ccgtttgcga
     1381 atgagcttga acttgtcgcc atctacgagc gcttcggcgg gtaaaggacg cgaattgtaa
     1441 ttcgacgcca tgacgaatcc gtaggcgccg gcgctcatga tggcgagcag atcgccttca
     1501 cgcagaggcg gcatcgcgcg atcgagtgcg aaaaaatcac ccgactcgca cactggcccg
     1561 acgatgtcga tcatctcttt cgtgctcttg ctcatactcg tgctcgtaat cggttcctcc
     1621 accggcacga tctcgtggta gctgtggtac agcgccggcc gaatcaggtc gttcatgccg
     1681 gcatcgacga tggcgaattt tttgcggccg gtttgtttga tgtaacggac gcgtgtgagc
     1741 aacacgccgg cgttgccgac gagcaatcgt cccggctcaa gtagaatccg cacgccgagt
     1801 tcgcgcagcg tcggcagaat cgcatccacg tattctttga tcgacaatgc gtgggttcgg
     1861 ctggcgtctg cacccccacc tggcgcttcg cgtcctcctc tccctgaggg agagcattga
     1921 gatgagggcc gtctcctctc gtcccaccat tctttcgcgc cgctttcgaa tgacgattcg
     1981 taaacaatgc cgagcccgcc gccgatgctg aagaactgga tcgcgtattt cgatttcaat
     2041 tcgccgacca gcggcgcgag cttgcgaatc gcggctgcaa atggctccgc ttcggtgatc
     2101 tgggagccga tatgcatttg cacacccacg atgtcgatct tgcgcatcct cgccgcgcgc
     2161 tcatagacgg cgccgagctg gtcgagcggg atgccgaact tgttctcgtg cgagcctgtc
     2221 gcgatgtatt cgtgcgtgtg cggatcgaca tcgggattgg cgcgcaatgc cacgggcgcg
     2281 cgaacgtttt tatcggccgc gattttgtcg atgtattcca gctcggtttc gctttcgaca
     2341 ttgaagctgt agacgcgctg ctcgagcgcg taatcgattt cctcgcgcga tttcccgacg
     2401 cccgcgaacg tgcatttgtc aggcgcgccg ccggcgttca ggacgcgaaa tagttcgccg
     2461 ccggagacga tgtcgaatcc ggcgccggca tcggccagta acttcaggac cgcgcgattc
     2521 gagttcgcct tgaccgcata gcaaatcagg tggtcgatcg gtgcgagcgc ggcgtcgagc
     2581 cgggtgtagt gatcgagaat ggttccggca ctatatacat aaacaggggt gccgaattct
     2641 gtggccacgc gcgcaagatc gacatcttcg cagtaaagct tgccttcgcg ataacggaac
     2701 gcgtgcatgc gctataacat agcaggcgtt cgccgtcgcg aatgcctctc gcactcatct
     2761 ccgccggcgc caaagcccga tgaacgcaat caaaacgagt agcacgccga aagtgcaaac
     2821 aatcgccgcg cccgaaggca ggtcccacca gtaggaaaga aacaagccgc cgatgccgcc
     2881 gatgagcgca acgatccagc cgatgagcag acgattgccg gtgcgatctg ccaggttaat
     2941 tccacaaacc gctggcacaa tcaggtaact aaacaccagg aggacgccgg cgagccggac
     3001 aaaactcgtc acggccaaac cgaagacggc atagaagaga aaatcccacc agcgcacgcg
     3061 cagtcctttg gcgtaggcac catcgcggtc gaaagaaacg agcaagaaat ttttgcgctg
     3121 cgcaaagtgg aacgcgccga cgagagcaaa cagcgcgaag cgctcccaaa cttcgcgcgg
     3181 cgtgacgaga agaatgttgc cgatgagcat atttttgatc tgctcatcgc cttcggccgc
     3241 gcggctgagc aagaggactg cggcagccgc ggcgacaacg taagcgattc caatgactgc
     3301 ttcctgcggg atcttgctgg tgcgttttcc ggttacggaa aaaatcgccg cgccaagcag
     3361 cgtaaacgcg agcgcgatgc tgaaagttgt ccagctttcc agatcgatat gaaggagcac
     3421 ggcgagacaa atcccgagcg atgccatttg ggccatggcg atgtcgatga aaatgatgcc
     3481 gcgttggacg acatgcagcc cgaggtaaac cagcaaccac ggcaaaagaa cgcaggccgc
     3541 gatcggccac agcatcactt cccacatgtc cttacaatcc gtgagtcgtg attcgcgatt
     3601 cgtgaatcgt tgtttggcgg ctcatttgag cgcggcggcg agacgggaga cgagcacgtc
     3661 gattagtttc acgtaagtat cggtgccggg aagaccgccg gggaactggc agaagtccac
     3721 cactttcgcg ccggttgcgc tcgcgacttt ctcggcgatg cggcggtcgt ggtatggctc
     3781 aacgatgact gctttcacct gctgcgcttt catttgcgcg atcacttcgg tcaaatgcga
     3841 gggcgacggt ggaatgcctg gtttcggctc caaaaagatg tcgatgttga ggccaaagcg
     3901 gtgcccgaag tacggccacg agtcgtgata cgccgcgaga tgctggcctt tgaagggcaa
     3961 catggccgca ccccattcct gtagtttcgc gttaatcgtg gcttcgaatt ttttg
//
LOCUS       DASTDG010000131         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1450416, whole genome shotgun sequence.
ACCESSION   DASTDG010000131 DASTDG010000000
VERSION     DASTDG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450416"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFO30_04250"
     CDS             complement(<1..315)
                     /locus_tag="VFO30_04250"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HET9418531.1"
                     /translation="MAGQTRVIRPDYPTSFKIHLTRFYGCQQIEQRKSIVEPRACVLN
                     RSRLLGAGMTEKATFGAGCFWGVEETFRNLKGVVNTSVGYAGGKKENPTYEDVCTDET
                     GHA"
     gene            580..1788
                     /locus_tag="VFO30_04255"
     CDS             580..1788
                     /locus_tag="VFO30_04255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9418532.1"
                     /translation="MRVAQPPRVEVRPRDPGLADWLKHYPAWLFSEQLYQSIELMERY
                     SIELAADLLRRLHLIDALGEWRSSEELCRALSFQPRFHFALSWILERLVETDCIKTQS
                     TGAKHRYRLRYAPWPPELERLREATLEIHPSNAATLDLLDCAASLYPAVARGEQSAEQ
                     NLFSAERIPLWLNYFNNDNLTYAVNNWVTAVLIANRVQDRSNIRILEVGAGAGSASEI
                     LLRWFEESGLLPRIQRYLITEPNAFFRRRAQRELAKRYVDLPLEWGALDLNAAWATQG
                     VAPGEFDFVFGVNVLHIAKDLLFSLSQARSALASDGWLVIGECLRPYPNQPIYPELMF
                     QILDSFADVDLEPEIRPNPGFLTPEQWRSAFARAGFENVNAEPEIDRIREVYPHFFTG
                     GICGQKIPGQ"
     gene            1785..3191
                     /locus_tag="VFO30_04260"
     CDS             1785..3191
                     /locus_tag="VFO30_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418533.1"
                     /translation="MTPQLSGAKPLDAYRWIKLPERGTPTSLRLSGWVALHIGRWAAR
                     LLLLPATLYFLISAHDARRASYEYLQRMRDGRRAPWWHVLRHFYRFGATILDRVYLLR
                     GEFGHFALTLHGRDILHRQIDSGKGCILLGAHLGSFEILRTLGVTQQEFPLKVLMDTA
                     HNENITRFLDALNPKIAHTVIAAEGADKLLRVKESLDAGFLIGMLGDRVATGDKTTQC
                     QFLGAPAIFPAGPILLAAIMHCPIILFFGLYRGGNRYEIFFEQFADDIALPREQRSEE
                     TQIWVQRYVARVEHYARLAPYNWFNFYPFWDQPRFREIASEIATMRGNNQEIDKTEIR
                     ALIPHSGSMCLLERVIEWDDRSIFCISNTHRDSHNPLRRDGRLAAVHAFEYGGQAAAV
                     HGGLRARAAGTTAAPGYVAALRDAHLHVSHLDEIDSPLNIRAVRLFGDEANTVYACQI
                     SAGDILLAEGRITIMPRA"
     gene            complement(3206..3949)
                     /gene="fabG"
                     /locus_tag="VFO30_04265"
     CDS             complement(3206..3949)
                     /gene="fabG"
                     /locus_tag="VFO30_04265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9418534.1"
                     /translation="MRFENQVAIVTGAGRGIGHALALRFASEGARVACVSRTEENAKR
                     TADELNEIRGGCAKAYAVDVADHGAVQNVGAEILDEFGKIDILVNNAGVARDDLVMRI
                     SVEDWDSVIETNLRGAFSFTQAVVRAMTRQRSGRIINIASVIGLIGNAGQTNYAASKA
                     GLIGFTKSLARELASRNITVNAVAPGFITTDMTSGLSDEIKTTIQAQIPLGRTGTPED
                     VANVVAFLASAEASYITGQTLCVDGGIVM"
BASE COUNT          810 a         1217 c         1158 g          928 t
ORIGIN      
        1 ggcgtggccg gtctcgtccg tgcaaacgtc ttcataggtc gggttttctt ttttgccccc
       61 cgcatagccg acactcgtgt tcacgacacc cttcagattt cggaacgttt cttccactcc
      121 ccaaaagcag cccgcgccaa atgtcgcctt ttcagtcata cctgcaccaa gtaaccggga
      181 acgattcagc acgcaagcgc gcggctcgac aattgatttt ctctgctcga tttgctggca
      241 tccataaaac ctcgtcagat gaattttgaa gctcgtcgga taatcaggtc tgatcacccg
      301 agtttgcccg gccatttccc cggcgcgccg atcgtgccag cggtagtgat tctcgatgaa
      361 gtgatggcgg ctgtaactga atggcgtgca gattcccgcc tcatcggcat acccgcagtg
      421 aaatttctgg cgccattgcg gcctgcccag ccatttacga tctgtttatc ttcgaccaac
      481 gaggcggaga gcgaaatcgg tttccgttgt catgtcgaag atcgtgtaat tgttgagggc
      541 cggctctgga tccggcgcgg ctcaagatag ctcgtgaaca tgcgtgtagc tcagccacct
      601 cgtgttgaag ttcggccgcg cgacccgggg ctcgcagact ggctgaaaca ttaccccgcg
      661 tggctgttca gcgaacaatt gtatcagagc atcgaactga tggagcgcta cagcatcgag
      721 ctcgctgccg atctgttgcg ccggcttcat ttgatcgacg cgctcggcga gtggcggtca
      781 tcggaggaac tttgccgcgc gctgtccttt caaccgcgtt tccattttgc cttgagctgg
      841 attttggagc gcttggtcga gacggactgc attaagacgc aaagcaccgg cgcaaagcac
      901 cggtatcggc tgcgctacgc gccttggccg cccgagctcg agcgcctacg tgaagcgacc
      961 ctggagatcc atcccagcaa tgcggcgact ttggatttgt tagactgcgc tgccagcctg
     1021 tatccagccg ttgcgcgggg cgagcagagc gctgagcaaa acctgtttag cgcagaacga
     1081 ataccgttgt ggctgaatta ttttaacaat gacaacttaa cttatgcggt gaacaactgg
     1141 gtcactgcgg tgctgattgc caatcgtgtc caagatcgat cgaacatccg catcttggaa
     1201 gtcggcgcgg gtgccggcag cgcgagcgaa attctattgc gctggtttga agaaagcggg
     1261 cttttgccac gcatccagcg ctatctcatc acggaaccga atgcgttctt ccggcgtcgc
     1321 gctcaacgtg agcttgcaaa acgatatgtc gatcttccat tggaatgggg tgcgctcgac
     1381 ctcaatgcgg catgggccac tcaaggcgtc gcaccgggcg agttcgattt cgtgttcgga
     1441 gttaacgtat tgcacatcgc gaaggatctt ctgtttagtc tgagccaagc gcgctcagcg
     1501 ctggcgagcg acggctggct ggttattggc gagtgcctgc ggccttatcc gaaccagcca
     1561 atttatccgg aactgatgtt ccagattttg gacagctttg cagatgtcga tctagagccg
     1621 gagattcgcc ccaatcccgg ttttctgact ccagagcaat ggcggagcgc atttgcgcgc
     1681 gccggctttg aaaacgtcaa cgccgagccg gagatcgaca ggatccgcga ggtctatcca
     1741 cattttttta ccggcggcat ctgcggccaa aagattcccg gacaatgact ccgcagcttt
     1801 caggcgccaa acctttggac gcgtatcgct ggatcaaatt gcccgagcgc ggcacaccga
     1861 catcgctccg cctcagcgga tgggtcgcat tgcacatcgg ccgctgggcg gcgcgccttc
     1921 tcctccttcc cgcaacgctg tattttctga tcagcgcaca tgacgcgcgg cgggcatcgt
     1981 acgaatactt acaacgcatg cgcgacggtc gccgcgcgcc ttggtggcac gttctccgcc
     2041 atttttatcg tttcggcgcg acgattttgg atcgcgttta cctgctgcga ggcgaattcg
     2101 ggcacttcgc cttgacgctt catggccgag acattctcca ccggcaaatc gacagcggca
     2161 aaggctgcat tcttttaggg gcgcacctcg gcagtttcga aatcctgcgc acgctcggcg
     2221 tcacgcagca ggagtttccg ctcaaggtgt tgatggacac cgcgcataac gaaaacatca
     2281 cccgcttcct tgacgcgctg aatccaaaga ttgcgcacac ggtcattgcg gccgagggcg
     2341 ctgataagtt gctgcgagtg aaagaaagcc tcgatgccgg tttcctcatc ggcatgttag
     2401 gcgaccgcgt cgcaaccggg gacaagacca cgcaatgtca attcctcggc gcgcctgcaa
     2461 tttttccggc cggccccatc ctgctcgctg cgatcatgca ttgtccgatc atcctgtttt
     2521 ttgggctcta ccgcggcggc aatcgttacg aaatcttttt cgagcagttc gctgatgaca
     2581 tcgctttgcc gcgcgagcag cgcagcgagg agactcagat ttgggtgcaa cgctatgtcg
     2641 cgcgcgtgga gcattacgcg cgtcttgcgc cttacaactg gttcaacttc tacccgttct
     2701 gggatcaacc gcgctttcgc gagatagcga gcgagatagc gacaatgcgc ggaaacaacc
     2761 aagagatcga caaaactgaa atccgcgcgc ttatccccca ctcgggttca atgtgcctgt
     2821 tggagagggt gatcgaatgg gatgatcggt cgatcttctg catcagcaat actcatcgtg
     2881 attcgcacaa tcctttacgg cgcgacggac gattagcggc cgtgcatgct ttcgaatacg
     2941 gcggccaagc cgccgcagtg cacggcggat tacgcgcccg cgcggcaggc acaaccgcgg
     3001 caccgggcta tgtagctgca ctgcgcgatg cccatttgca cgtgagccat cttgatgaaa
     3061 tcgattcgcc gctcaacatc cgcgcggtcc gcttgtttgg cgatgaagct aacaccgttt
     3121 atgcatgcca gatctcagcc ggcgacattc ttctggccga ggggcgcatc acaatcatgc
     3181 cacgcgccta gcggcaatga atgagctaca ttacgatccc gccgtcgacg cagagcgtct
     3241 ggccggtgat gtagctcgct tcggccgacg cgaggaaggc gacgacattc gcgacatcct
     3301 ccggcgttcc ggtcctgccg aggggaattt gcgcttggat tgtggtttta atttcatccg
     3361 acaatcccga ggtcatgtca gtcgtgatga agccaggcgc gaccgcgttt acggtgatgt
     3421 tgcgactggc cagctcgcgc gcgagcgatt tcgtaaatcc gatgaggcca gctttgctcg
     3481 cggcgtagtt cgtttggccg gcgttgccga ttaatccgat caccgaagcg atgttgatga
     3541 tgcggccgct gcgctgccgc gtcatcgcgc gcaccactgc ttgggtaaag ctgaatgcgc
     3601 cacgcagatt tgtctcgatg acgctgtccc aatcctcgac ggagatccgc atgacaaggt
     3661 cgtcgcgagc cacgcccgcg ttgttcacga ggatgtcgat cttgccgaac tcatccagaa
     3721 tttctgcacc gacgttttgc accgcgccgt gatcggcgac atcaacggcg taagcttttg
     3781 cgcaaccgcc gcggatttcg tttaactcgt cagccgtccg ctttgcgttt tcttcggtcc
     3841 ggctgacgca ggcgacccgc gctccttcag acgcgaatcg cagcgcgagc gcatggccga
     3901 tacctcgacc cgcgccagtc acaatggcga cctgattctc aaatctcatc aacacgcgcg
     3961 tttaggatgc aaagatccgg agaaaggcaa cctcactcgc gagcgcttcc tggatattgc
     4021 ggcccagatt atcgcgtaag tcctccagcc agcgaatccg gcgcagcact tcggcgctcg
     4081 taaagcgctt tgccgcctcg gtagtttgat gtt
//
LOCUS       DASTDG010000132         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1250322, whole genome shotgun sequence.
ACCESSION   DASTDG010000132 DASTDG010000000
VERSION     DASTDG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250322"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFO30_04270"
     CDS             complement(<1..902)
                     /locus_tag="VFO30_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET9418535.1"
                     /translation="MHPEDRPVSAEAPGRVEILGNHTDYNEGVVLAAAIDRAVRVCGN
                     VQNDGCISLRSTSYPPTEIGISQLRPQQGELRWANYPLGVASELLTAGISIRGFSAVI
                     ESDLPAGIGLGSSAALAVATAFFLLKLNKCELPPLELAKICQRSEHQFSGVQAGLLDQ
                     VTSIFAKAGELIFFDCRTEEVHTISFPNDLSLVVADSGIKRQLARSEYNRRREQTRAA
                     AQALGVRALRDIFPAQLNAHVDLAPLFRRRAAHIIGENERVWRAVELLRRGEGVSLGD
                     LMNASHESSRTNFENSTPELDHLV"
     gene            992..1558
                     /locus_tag="VFO30_04275"
     CDS             992..1558
                     /locus_tag="VFO30_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418536.1"
                     /translation="MRKTPFCVPPIVVIVGSFLSASGVHAARLGSTHSQFTFRDGNGK
                     AETVPVESNYYPKKITSPMAKLDRRLDPRLLRAVTVAEERAHAHSREQCWHYVKDALL
                     AAGAVSSRPKTLLAKEAGDELARNYGFTRLSVNDPYAAPVGAVLVYDAKRAAGHVEIR
                     TRDGFVSDFRSKTPSPRRLLAVYAKLSS"
     gene            1733..2326
                     /locus_tag="VFO30_04280"
     CDS             1733..2326
                     /locus_tag="VFO30_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET9418537.1"
                     /translation="MKAKSTSFAQLLSVAIAAGAFCTVGSICFAQGRDTTTSTATNPH
                     LQPQTATTAQTKVSPKDNTFLVNASTSNAVEVEDGKIGQRAGSAEVKKVAGRMVAEHS
                     KANKELIDLAKRKGVALNTSGITAEIKMAPGPEFDRQFLAMLETNHKKAIADFEKEAK
                     SGDDSDLRAYANRQLPTLREHLTMVEQAIRKTGSVSR"
     gene            2393..3130
                     /locus_tag="VFO30_04285"
     CDS             2393..3130
                     /locus_tag="VFO30_04285"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418538.1"
                     /translation="MSGQSVRIAMWSGPRNVSTAMMRAWGNRADTVVVDEPFYAYYLK
                     ATGKKHPDAEEVIAQGETDWRKIVACLTKEPIPGGKSVFFQKHMTHHLLPEVDREWIV
                     DLANCFLIRDPRDVILSYLKKNPDPALDDLGFVQQREIFEFVRERTASIPLVVEAKDV
                     LDNPERILRLICDALGVEFDSTMLSWPPGLRETDGIWGKHWYTEVARSTSFHRYEPRE
                     GAVPDRLRKIHERCRDCYEALYQHRLH"
     gene            3244..>3908
                     /locus_tag="VFO30_04290"
     CDS             3244..>3908
                     /locus_tag="VFO30_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9418539.1"
                     /translation="MLQKLHERNRDLIVNINGQLMHRDQAGVSPFDSAVQGGDAVWEG
                     LRLYNGRIFKLHEHLDRLERSARALSFVSLPPREKIIDELKRTLAANKMRDGVHIRLT
                     LTRGVKITSGMDPRLNQSGQTLIVVAEHKPPVYSKDGLKLITATVRRPPPEVLDARIH
                     HANLLNSILAKIEANSAGADDALMLDTNGFVAETNATNVFIVRKGDLATSRAVACPEG
                     ITRA"
BASE COUNT          852 a         1154 c         1117 g          785 t
ORIGIN      
        1 tcgacaagat gatcgagctc gggtgtgctg ttctcgaaat tcgtgcgcga actttcgtgc
       61 gatgcattca tcaaatcgcc tagactgacg ccctcgccgc gtctcagcag ctcgacggca
      121 cgccagacgc gttcgttttc gccaataatg tgcgcagcgc gccgccggaa gagcggtgca
      181 agatcgacat gcgcatttag ctgcgcgggg aaaatgtccc gcaacgcgcg gacgcctagt
      241 gcttgcgcgg ccgcgcgcgt ttgttcgcgc cgccggttgt attcactgcg ggcgagctga
      301 cgttttattc ccgagtcagc cacgaccagc gagagatcgt ttggaaatga gatcgtgtgt
      361 acctcttccg tgcggcagtc gaagaagatg agctcacccg ctttcgcaaa gatcgatgtg
      421 acctgatcga gcaagcccgc ctgaacgccg ctgaactgat gctcgctgcg ctgacaaatc
      481 ttcgcgagct ccagcggcgg cagttcgcac ttgtttagtt tgaggaggaa gaacgcggtt
      541 gcgacggcga gcgccgcgga gctgccgagg ccgatgccag cgggcaggtc gctttcgatc
      601 acagcggaaa atccgcggat tgaaatgccc gctgtaagaa gttcgctcgc gacgcccagg
      661 ggataattgg cccagcgcaa ctccccttgt tgcggtcgca gttgtgaaat gccgatttca
      721 gtgggcgggt acgatgtcga tcttaatgag atacagccat cattctgcac attgccgcaa
      781 acgcgtaccg cgcgatcgat cgccgcggcg agtactaccc cttcattgta gtccgtgtgg
      841 ttcccgagga tctcgacgcg gcccggtgct tcagcggaaa ctggccggtc ttcagggtgc
      901 atacggcaat ttcgcaggcc gagaaaattt cacttttgtc gaaaaaagcg gttgcggcgg
      961 cagtaatttt gctcgatagg acgcgctcgc tatgcggaaa acccccttct gcgtcccacc
     1021 cattgtcgtc atcgtcggtt cattcctcag tgcgagcggt gttcatgccg ctcggcttgg
     1081 ttcgactcat tcgcagttca cgtttcgcga tggtaacgga aaagccgaaa cggttccggt
     1141 cgaatcaaac tattacccaa agaagattac ttcgccgatg gcgaaattgg atcgccggct
     1201 cgatccgcga ctgctgcgcg ctgtcaccgt ggccgaggaa cgcgcgcatg cgcattcgcg
     1261 cgagcaatgc tggcattatg tcaaggacgc gcttctcgca gccggcgcgg tgagctcccg
     1321 gccaaagacg ctcctcgcga aagaggccgg cgatgagctg gcgcggaact acggctttac
     1381 tcgcctatcg gtcaacgacc cgtatgcggc gccggttggt gcggtgctcg tttacgatgc
     1441 gaaacgcgcc gctgggcacg tcgagatccg cacccgggac ggattcgtca gcgactttcg
     1501 ttcaaaaacg ccttcgccgc ggcggttgct tgccgtttac gctaagctgt cgtcctaaca
     1561 tccaatcccg accgaccagc gccgtgacct ccgcgcctgt gccgcgacgc gtgtaggcgg
     1621 tgttgcacgg cgccagcacg ccagcaatca gtctcgcacg tccaattgcc ggtgaatgcg
     1681 cgaactcctt ttgctgaccg ttttcggtca ggacgtaaac tggactaatt ctatgaaagc
     1741 aaaatcgaca tcgttcgcac agttgctgag cgtggcaatt gcggctggcg cattctgcac
     1801 ggtcggctcc atctgtttcg ctcaggggcg ggacaccacg acctcgaccg ccactaatcc
     1861 gcatttgcag ccacagaccg ctaccactgc ccagaccaag gtcagtccca aggacaacac
     1921 ctttctggtc aacgcttcca cgagcaatgc cgtcgaagtc gaggatggaa agatcggcca
     1981 gagagccggg agcgccgagg tgaagaaggt ggcaggacgg atggtggctg agcattcgaa
     2041 agccaacaag gaactgatcg atctggccaa gagaaaaggc gtcgcactca acaccagtgg
     2101 tattacggcc gaaatcaaga tggcgcctgg accagagttc gatcggcagt ttctagccat
     2161 gctcgaaacc aaccacaaaa aggccatcgc cgattttgaa aaggaagcaa agagcggcga
     2221 tgactcagac ctgcgtgctt atgcaaatcg gcaattgccg accctacgcg aacacctcac
     2281 aatggtggag caggcaatcc gtaagaccgg ctccgtgagt cgataaagcc ggcgccgcct
     2341 gccgttaggt tcacgctgac aagctgccgg cgctgatgca atctcagcgc caatgtcggg
     2401 tcagagtgtt cggattgcca tgtggtcggg cccgcgcaac gtctctaccg cgatgatgcg
     2461 cgcctggggc aatcgggccg acaccgttgt cgtcgacgag ccgttttacg catattatct
     2521 caaagcgacc ggcaaaaaac atcccgacgc cgaagaagtc atcgcgcaag gcgagaccga
     2581 ttggcgaaag atcgtcgcgt gcctaacgaa ggagccgatt cccggtggca agagcgtctt
     2641 tttccaaaag catatgaccc atcacctgct accagaggtc gatcgagaat ggattgtcga
     2701 tcttgccaac tgtttcctca ttcgcgaccc gcgcgacgtt atcctctctt acctcaaaaa
     2761 gaatcccgac ccggcgctgg acgacttggg tttcgtccag caacgcgaaa tcttcgagtt
     2821 cgtgcgtgag cgaaccgcgt ccattccgct ggtggttgaa gccaaggatg tgctggataa
     2881 tcccgagcgc atattgcggc tgatatgcga cgcgctgggt gtcgagttcg attccacgat
     2941 gctctcatgg ccgccggggt tacgcgagac cgacgggatt tggggcaaac attggtacac
     3001 cgaggtcgca agatcgacat cattccaccg ctatgaaccg cgtgaaggtg ccgtgcccga
     3061 ccggctccgc aagattcacg agcgctgccg cgactgttac gaggcgcttt accaacaccg
     3121 gctgcactga tgatcatccg cagattttgc agatttacac agattgaagg tttcaacgac
     3181 agctcataca aattcagctc tgaataatct gcgagaatct gtgtaatctg tggatcaata
     3241 aagatgctgc agaaattgca tgagcggaac cgcgatctga tcgttaacat taacggtcag
     3301 ctgatgcacc gcgatcaggc tggcgtcagt cctttcgatt cggcggtcca gggaggggac
     3361 gcagtctggg agggtctacg gctctacaac ggtcgcatct ttaaattgca cgagcatctc
     3421 gaccggctcg agcgctcggc gcgcgctctt tcgttcgtta gtcttccgcc acgcgaaaaa
     3481 atcatcgacg aactcaagcg cacactcgcc gcgaacaaaa tgcgcgacgg tgttcacatt
     3541 cggctcaccc tcacccgcgg ggtgaagatc acctccggca tggacccgcg cctcaatcaa
     3601 agcgggcaga cgttgatcgt tgtcgccgaa cacaaaccac cggtttactc gaaagacggt
     3661 ttgaaactca tcaccgccac cgtgcgccgg ccgccgccgg aagttctcga tgcccgaatt
     3721 catcacgcca atttgttgaa ctcgattctg gccaaaatcg aagcgaacag cgccggcgcg
     3781 gacgacgcgc tcatgctcga caccaacggc ttcgtggctg aaacaaacgc gaccaacgtt
     3841 tttatcgtgc gcaaaggcga cctcgccacg agccgcgctg tcgcctgccc ggagggaatc
     3901 acccgcgc
//
LOCUS       DASTDG010000133         5833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1590945, whole genome shotgun sequence.
ACCESSION   DASTDG010000133 DASTDG010000000
VERSION     DASTDG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590945"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFO30_04295"
     CDS             <1..582
                     /locus_tag="VFO30_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9418540.1"
                     /translation="VLWDKETSKIVNNCEDDICRMFNNIFDAFARDKDVDLFPKEIEA
                     EHNKLNDFLYDNVNNGVYKAGFATRQRPYEISCRKLFEALDQLEQRLTEIRYLFGNRI
                     VEADWRLFCTLVRFDVVYYGHFKCNLRRIIDYPSLQGYLMDLYQQPRIAETVNFEHIK
                     RHYYMTHEEINPTRIVPLGPAIDLSKPHRREIL"
     gene            complement(593..1642)
                     /locus_tag="VFO30_04300"
     CDS             complement(593..1642)
                     /locus_tag="VFO30_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HET9418541.1"
                     /translation="MKIRTSIARICSTLVLCLATLPACHRTQPEVKDLPGLPPVAETE
                     DKIVSEIWQFRLKTRSLYNASKFDELETLASQLRSEPARFGNGSWKIYQFYEALRCRS
                     DEPESMWQLHSTIHENWDAAKPRSITSYVAHADFFAEYAWHARGSGYANKVTKEGWRL
                     FGERLAKAKELLDASGGFGTKCPVWWVAYMRVALGQGWSWDDHEKLFREAKAFEPEFW
                     PYDIAKAKYLLPRWHGQPGEWEYALGSETDRSKPQTIEAYARVVDALQNYYKNIFRES
                     HASWPLTCDGFALMRARYPQSLEILSDYCWLACLAEDRVLARQLFKELGGRMLKSIWH
                     DQKNFRRYRNWAYSQ"
     gene            complement(1642..2451)
                     /locus_tag="VFO30_04305"
     CDS             complement(1642..2451)
                     /locus_tag="VFO30_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9418542.1"
                     /translation="MKLDNCSALITGASAGIGREFARQLAARANLLVLVARRGQRLEQ
                     LREELSARNPRLKVHTRILDLRDASQIDALVQWLDREKIDIDFLINNAGLGDLGPFAS
                     SDPARNDAMLLVNVVALTTLTRKLLPQMLARKRGAILNVSSSAGFLPIPECAVYAATK
                     AYVNSFSEALRADLRGTGVSVCALCPGPVHTEFQEVASRPGRKVKPGPGPEFVQVAVE
                     QVARDALTAVEADRPLVIPGLVMKLGMFFVRLTPMSILRRVSGRRGQGPNG"
     gene            complement(2438..3142)
                     /locus_tag="VFO30_04310"
     CDS             complement(2438..3142)
                     /locus_tag="VFO30_04310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9418543.1"
                     /translation="MRLNRYLAAAGLGSRRHCDELIATGRVTINGQPCTDFSAQPSAR
                     DHVKVDGKLVHVDLPITVALHKPAGFVSTKSDPHARDTIFDLLPPKFQRLFNIGRLDA
                     QSEGLILLTNDGELAQRLTHPRYKVDKEYEVTLDRPWKPALAPKLLRGVVLDGQRAQL
                     AQLHPIRPARLRVVLRQGINRQIRRMFHALGYNVKRLVRVRVGNLRLGDLPRGHWRVL
                     TKPELRSLQTPDNETG"
     gene            complement(3226..3381)
                     /locus_tag="VFO30_04315"
     CDS             complement(3226..3381)
                     /locus_tag="VFO30_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HET9418544.1"
                     /translation="MSQHRSLKGASTIAAKRNVLKRFERVELLKKRGQWKEGRKVVGL
                     PKTKPDV"
     gene            complement(3450..5243)
                     /locus_tag="VFO30_04320"
     CDS             complement(3450..5243)
                     /locus_tag="VFO30_04320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9418545.1"
                     /translation="MHVGANTLRIEETLLEPSAINPPPVRHALLVCLLALTVLLHVAT
                     IGWGDLYSQTDGQYAGAAREMIAEHHLLLPTNDHVPRLQKPPLLYWLLIGSFKLFGVT
                     AAAARLPVALATVATVALTFLIGERLAGYWRGFLAGLIYLCSCGVFLLGRILMPEPVF
                     SALLAGAIYCGLRGYQSRGRRAGWFIGVWLCCALATLAKSPLGLIYVAAVFLLLSIFF
                     REARMRFRALLRWEYLSIFCLLVVPWHMWAERHFPGYLRQLLGAEWIGHVRGLSDATH
                     DYEGVPPLQFLTLHFVWWFPWSIALLPALFLANRRIFRPREIHFGDALPLCWMAVAFV
                     PLLLVGQRQDYYSMSMWSAFALWAASAWERAPDKLRATGVVAVTLAGLLVIALALFLP
                     LLAREMHGGWGSMDMRWTAWKALRAMPTATWLSLRSMLFISGFSLVLFSGLGFYLLHK
                     KHDRFAVIALAAAMVPTGLAMMDGVVRIAPFFSLADAARLLNSRVATDHEVLFEGPLE
                     DSSSLVFYLNRSFSLVNQNPAKEAPFGGGAGETFVDEAGVFERWGDAEPVYLIVEQSR
                     AAHWQALLIQRFHIFHQVATCGTYAVLSNQL"
     gene            complement(5250..5711)
                     /locus_tag="VFO30_04325"
     CDS             complement(5250..5711)
                     /locus_tag="VFO30_04325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9418546.1"
                     /translation="MMTSERPSIRTEVQVMFFDTDCGGVVSNIAYLRFIEIARTLLAE
                     ELGLRLAEMATSQQYPVVVRTEIDYRRAAKLADRLVIEGWLDKLDRARFWCAFRILRP
                     QDGALIAECRQMLVLIKMPTATLLRLPESWEKYRANHSPQSAAAQHVALNA"
BASE COUNT         1295 a         1716 c         1674 g         1148 t
ORIGIN      
        1 gtgctttggg acaaggagac aagcaagatc gtgaacaact gcgaggacga catctgccgg
       61 atgttcaaca acatcttcga cgcgtttgcc cgagacaaag atgtcgatct tttcccgaag
      121 gagatcgaag ccgagcataa caagctaaac gatttcctct acgacaacgt gaacaatggc
      181 gtctacaagg ccgggttcgc gacgcgccag cgcccgtatg aaatctcctg ccgcaaatta
      241 ttcgaggcgc tcgaccaact cgagcagcgc ctaacggaga ttcgctacct gttcggcaat
      301 cgtattgtcg aagccgattg gcggctcttt tgcacgctcg tgcggttcga cgtcgtttat
      361 tacggccatt tcaaatgcaa ccttcgccgg attatcgatt acccgagcct ccagggctac
      421 ctgatggatt tgtatcagca gccgcgcatc gccgagacgg taaacttcga gcacatcaag
      481 cgtcattatt acatgacgca cgaggaaatt aatccaacgc gcatcgtccc gctcggtccg
      541 gcaatcgatt taagcaaacc gcaccgtcgc gagattcttt aagcgccgga atttactggc
      601 tgtacgccca gttacggtaa cgtcgaaagt ttttctggtc gtgccagatg cttttgagca
      661 ttcgtccgcc gagttctttg aagagttgtc gcgccagaac gcggtcctcg gccagacaag
      721 ccaaccagca gtagtcgctc aaaatttcca aggactgcgg atatctagct cgcatcagcg
      781 caaagccgtc gcaagtcagg ggccaggaag catgcgactc ccggaatata tttttgtaat
      841 aattctgcaa cgcatcgaca acgcgcgcgt atgcttcgat agtctggggc tttgaccgat
      901 cggtttccga gccaagagcg tattcccatt ctcccggttg tccgtgccat cggggcaaga
      961 gatatttcgc cttggctatg tcatagggcc aaaactcggg ctcgaaagct ttggcctccc
     1021 ggaagagctt ttcgtggtcg tcccagctcc aaccctgacc caacgccacg cgcatgtagg
     1081 caacccacca gactgggcat tttgttccga aaccgccgga cgcatcgaga agctctttcg
     1141 ctttggccag ccgttcgccg aacagacgcc agccctcttt tgtcacctta ttggcgtacc
     1201 cgctgccgcg agcatgccag gcgtactccg cgaaaaaatc agcatgagct acgtacgagg
     1261 taatcgagcg cggcttggct gcgtcccaat tttcatgaat tgtcgaatga agctgccaca
     1321 tgctctccgg ctcgtccgat cgacaacgca gcgcttcgta gaactgatat attttccacg
     1381 agccgttacc gaagcgcgct ggctcggagc gtagctggct cgctaaggtt tcaagctcat
     1441 cgaacttgct cgcgttgtag agacttcgcg ttttcagacg gaattgccag atctcgctga
     1501 cgatcttgtc ctctgtctct gcgaccggcg gcaagccggg taaatccttg acctctggtt
     1561 gcgtgcggtg gcacgccggc agtgtcgcta ggcaaagcac gagcgtggag cagatacgag
     1621 caatggaggt tcgtatcttc atcaaccatt cggtccttga ccgcgtctgc cgctgactct
     1681 gcgcaagatc gacatcggcg tgagccgcac gaaaaacatg cctaacttca tcaccagacc
     1741 gggaatgacg agcggccggt cggcttcgac cgcggtgagg gcatcgcgcg cgacctgttc
     1801 gaccgcgacc tgaacaaatt cagggccggg gccaggtttc actttacggc cagggcgact
     1861 cgcgacttcc tggaattcgg tgtggaccgg cccggggcac aacgcgcaca cgctcacccc
     1921 cgtgccgcgc aaatcggcgc gtaacgcttc gctgaaactg ttgacgtatg cttttgttgc
     1981 ggcgtagacc gcgcactcgg gaatcggcag aaatccggcg gatgagctca cgttcaagat
     2041 tgcaccgcgc ttccgcgcca gcatttgcgg cagcaacttt cgcgtcaacg tggtgagcgc
     2101 aaccacgttg acgagcaaca ttgcgtcatt acgtgccggg tcggaagaag cgaacgggcc
     2161 gagatcgccc aggccggcgt tattgatcaa aaaatctatg tcgatctttt cccgatcgag
     2221 ccattgcacc aaagcgtcga tttgtgatgc gtcgcgaaga tcgagaatcc gcgtgtgcac
     2281 tttcagccgc ggattgcgcg cgcttaactc ctcgcgcaat tgttcgagcc gttgaccgcg
     2341 acgtgcaact agcacaagca ggttcgcacg cgccgcgagt tgccgagcaa attcgcgacc
     2401 aatcccggcc gaagcgccgg tgatcagcgc gctgcagtta tccagtttca ttgtcgggcg
     2461 tttgcaatga ccggagttct ggcttcgtca gcacccgcca gtggccgcgc ggcaaatcgc
     2521 cgagccgaag atttccgacg cgcacgcgaa cgaggcgttt cacgttatag ccgagcgcgt
     2581 ggaacatgcg gcgaatctgg cggttgattc cctgtcgcag aacgacgcgc agacgagccg
     2641 ggcggatggg atgcagctgc gcgagttgcg cgcgttggcc atcgagaaca actccgcgca
     2701 gcaatttggg cgcgagcgcc ggcttccatg ggcgatcgag agtgacttcg tattccttgt
     2761 cgaccttata gcgcggatga gtgagccgtt gcgccaactc gccgtcgttt gtgagcaaaa
     2821 tcaacccttc actctgcgcg tcgagtcgac cgatgttgaa gaggcgttga aatttcggcg
     2881 gcaagagatc gaaaatcgtg tcacgcgcgt gcgggtcgct cttggtggaa acgaaaccgg
     2941 ctggtttgtg caacgcgacc gtgattggaa gatcgacatg caccagtttg ccatcgactt
     3001 ttacgtggtc gcgcgcgctc ggttgcgcgc tgaagtcggt gcacggctgt ccgttgatag
     3061 tgacgcggcc ggtggcgatg agttcgtcgc agtgtcgccg cgagccgagt cccgcggcgg
     3121 cgaggtaacg gttcaggcgc atgggaatcg ttaaaccgtg aattcgctga atcggattcg
     3181 tccagccgat ttaacgatgt aacgctttaa cgattcacgt ttccgctaca cgtccggctt
     3241 ggtcttggga aggccgacca ctttgcggcc ttccttccat tggccgcgct ttttcaacag
     3301 ctcgacgcgc tcgaaacgtt tcaggacgtt tcgcttcgcg gcaatggtgc tggctccttt
     3361 caagctgcga tgttgcgaca tagcgagact gccaatctgc agtgaacgcc gaccttaacg
     3421 caccgtgtcc gtttttcaaa tcactcgcgc tacaactgat tgctgagcac ggcataggtg
     3481 ccgcaggtcg cgacctgatg aaaaatgtgg aagcgctgga tgagcaatgc ttgccaatgt
     3541 gccgcgcgcg attgttcgac gatcagatac accggctcgg cgtcgcccca gcgctcgaag
     3601 acaccggctt cgtcgacaaa tgtctcgccg gcaccgccgc cgaacggcgc ttctttggca
     3661 ggattttggt tcacgagtga aaagctgcgg ttcaaataaa agacgagact gctactgtcc
     3721 tcgagcggtc cctcgaagag gacctcgtgg tcggtcgcga cgcgcgagtt gaggagtcga
     3781 gctgcatcag ccagtgagaa gaacggcgcg atgcgcacca ctccgtccat catggccaat
     3841 cctgtcggaa ccatcgccgc cgcgagggcg atgacggcaa accgatcgtg ctttttgtgc
     3901 agcagataga atccgagccc ggaaaagagc accagggaga agccgctgat gaacaacatc
     3961 gaacgaagac tcagccacgt cgccgttggc atcgcgcgca gtgctttcca tgctgtccac
     4021 cgcatatcca tgctgcccca ccccccgtgc atttcgcgtg caaggagtgg cagaaacaga
     4081 gcgagcgcta tgaccagcag cccggcgagg gtcacggcaa cgactccggt ggcgcgcagt
     4141 ttgtcaggcg cccgttccca cgcgctggcg gcccacagcg cgaacgcgct ccacatgctc
     4201 atcgagtaat aatcctggcg ttgaccgacg agcagcagtg gcacgaaggc aactgccatc
     4261 cagcaaagtg gcagcgcgtc gccaaagtgg atttcgcgcg gccgaaaaat gcgtcggttc
     4321 gcgaggaaca acgctgggag cagagcaatt gaccatggaa accaccagac gaaatgaagg
     4381 gtgagaaatt gcagcggcgg gacgccctca taatcgtgag tggcatccga taagccacga
     4441 acatggccga tccattccgc gcctaacaac tgccgcagat atccagggaa gtgccgctcg
     4501 gcccacatgt gccacggcac gacaagcaaa caaaagattg ataagtactc ccagcgcagc
     4561 aaggcgcgaa aacgcatgcg cgcttctcgg aagaagatgg aaagcagaag aaaaaccgcg
     4621 gccacgtaaa tcaatccgag cggacttttg gccagcgtcg ctagtgcgca gcagagccag
     4681 acgccgataa accagcccgc gcgccgacca cgcgactgat aaccgcgcaa gccgcaatag
     4741 atcgcgccgg caaggagcgc gctaaatacc ggttccggca tcagaattcg gccaagcaaa
     4801 aatacgccgc acgagcagag ataaatcagc cccgccagga agccgcgcca gtagccggcc
     4861 agtcgttcgc caatcaaaaa cgtgagtgca acagtcgcta ccgtcgcgag ggcgaccggc
     4921 aggcgcgcgg cggcggcggt gacaccgaag agcttgaacg atccgatgag tagccagtag
     4981 agcaacggcg gtttttgcaa tcggggcacg tggtcgttcg tcggcagaag caggtgatgc
     5041 tcagcgatca tctcacgcgc agcgccggcg tattggccgt cggtttggct gtagagatcg
     5101 ccccatccga tggtcgcgac gtgcaacaat accgtcagcg ccagcaaaca gaccaacagc
     5161 gcgtggcgaa ccggcggagg attgatcgcc gacggctcga gcagcgtttc ctcgatcctc
     5221 agcgtgttcg cgccgacgtg cattgctcgt taggcattta gcgcaacatg ctgcgccgct
     5281 gcgctctggg gtgaatgatt ggcgcgatat ttctcccaac tctccggcaa acgcagcagt
     5341 gtagccgtcg gcatcttgat gagcacgagc atctgccgac actcggcgat cagcgcgccg
     5401 tcttgcggcc gaagaatccg aaacgcgcac cagaaccgcg cgcgatcgag tttgtcgagc
     5461 caaccttcga tgacgaggcg atcggctaat tttgccgccc ggcgatagtc aatctcggtg
     5521 cgaacaacga ccggatattg ctggctcgtt gccatctcgg cgaggcgcaa gcccagttct
     5581 tccgctagca gtgtccgcgc aatttcaatg aaccggagat acgcaatatt ggagacgacc
     5641 ccgccgcaat cggtatcgaa aaacatcacc tgcacctcgg ttcgaatcga cggccgctct
     5701 gaagtcatca tttggaagtt atcacgaact cttctgcaag atcgacaata cagcatcggc
     5761 cacttcccgg gggttcacag ctttcataca ccgaaaatca atcggacatt cgcgtaagaa
     5821 acacgggctg cat
//
LOCUS       DASTDG010000134         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1359451, whole genome shotgun sequence.
ACCESSION   DASTDG010000134 DASTDG010000000
VERSION     DASTDG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359451"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="VFO30_04330"
     CDS             complement(<1..1749)
                     /locus_tag="VFO30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9418547.1"
                     /translation="MPAQVIKISGARQHNLKNLHVELPREKLVVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDQMEKPDVDFIEGLSPAIAIEQRSAGANPRSTIAT
                     TTEIYDYLRVLFSAVGQPHDPQTGEPIYRQTPQQIVGQILAYGPDIKVIVLAPLIRDQ
                     AGEFRDVLEKLKREGFVRARVDGEIVELDRPQPIRLKKNARHTIEAVVDRLVIRDGVR
                     SRLTDSVETALKWGGNHVVVLEQTPNPPSQSSGAAGVQRSTSNAKTSESWIEHRHSTD
                     YGNAETGFTLGELTPKHFSFNSHLGACPACHGLGTQLVIDPELMISDQTKTIAEGAIV
                     PWRRGTKRMQAYYRQWQGALVKHFQVDEDARFADLPNEFKNALYFGTNGRPIETNFGG
                     NGEKKVARPFEGLVPQMQRLYETTQSEFTRNRIRSFMTRVPCKTCGGARLKPEILAVT
                     IRGACVSLADRNRDGCATIKLNIHQFSELSVEAAARYIDKLELSAQQETIVADVVQEI
                     RSRLEFLVEVGLGYLTLNRESGTLSGGEAQRIRLATQIGSGLAGVLYVLDEPSIGLHQ
                     RDNARLLGTLRKLRDLG"
     gene            2190..2537
                     /locus_tag="VFO30_04335"
     CDS             2190..2537
                     /locus_tag="VFO30_04335"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9418548.1"
                     /translation="MLFKTLCDPTRLRLLNLLACGETCVCELTDTLRVVQPKVSRHLR
                     HLERAGLVEARRNGKWIHYRWARHGDPLVQHVLIGLRDWMKQDARMRHERVRREKVCC
                     RVNRRKPKEVKTK"
     gene            2534..3010
                     /locus_tag="VFO30_04340"
     CDS             2534..3010
                     /locus_tag="VFO30_04340"
                     /inference="COORDINATES: protein motif:HMM:NF040413.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HET9418549.1"
                     /translation="MTTEEFISGLRRTPDQPLLFVDLAGHVVHRGYHLTEIKAASFDT
                     VDCGGQVNHWRETIVQLWVPEHAEATYMTAGKFLKIFERVSGMVPLDTEAELRIEYGD
                     ENFFPSIYHVRAVTYDGDTTRVLLEPPSMTCKARDRRVASASEPCCETTAASCCAA"
BASE COUNT          612 a          923 c          867 g          661 t
ORIGIN      
        1 gccgaggtcg cgcagtttcc gcagcgtccc gagcagccgc gcgttgtcgc gctgatgtag
       61 gccgatgctc ggctcatcaa gaacgtaaag cacgccggca agacccgagc cgatttgcgt
      121 cgccaggcgg atacgttgcg cttcgccgcc gctcaacgtc ccactctcgc gattgagcgt
      181 gagataaccg agcccgacct cgacgagaaa ctccaagcgc gaccggatct cctgcaccac
      241 gtccgccacg atggtctcct gctgcgcgct caactcgagt ttgtcgatgt aacgcgccgc
      301 tgcctcgacg ctaagctcac tgaattgatg aatattgagc tttattgtgg cgcaaccgtc
      361 tcggttgcga tctgcaagcg agacgcatgc gccacgaatc gtcaccgcga ggatctccgg
      421 cttcagccgc gcgccgccgc aggttttgca cggcacccgc gtcatgaatg agcgaatgcg
      481 attgcgcgtg aattcacttt gcgttgtctc atagagccgc tgcatctgtg gcaccaagcc
      541 ttcgaatggc cgcgccactt tcttttcgcc gttgccgccg aagttcgttt caatcggcct
      601 gccgttcgtt ccgaaataaa gcgcgttttt gaactcgtta ggcaaatcgg cgaagcgcgc
      661 atcttcgtcc acttggaaat gtttcaccag cgcgccctgc cattgccggt aatacgcctg
      721 catccgcttc gtcccgcgcc gccacggcac aatcgcgccc tccgcgatcg tttttgtctg
      781 gtctgaaatc atcaactcag ggtcgatcac cagctgtgtg cccaacccat gacacgccgg
      841 acacgcgccg agatggctgt tgaacgaaaa atgtttcggc gtcagctcgc ccagcgtgaa
      901 tcccgtctcc gcgtttccgt aatcagtgga gtggcgatgc tcaatccagc tttctgatgt
      961 tttagcgttg gacgttgagc gttgaacgcc tgccgcgccg gagctttgcg aaggcgggtt
     1021 gggcgtttgc tcgagcacaa caacgtgatt gccgccccac ttgagcgccg tctctaccga
     1081 gtccgtcaac cggctgcgaa caccgtcacg gataactaaa cgatccacga ccgcttcgat
     1141 cgtgtgtcgc gcgttcttct ttagccgaat gggctgcggc cgatccagct cgacgatctc
     1201 gccatcgacc cgcgcccgga caaatccttc gcgcttgagt ttctccagca catcgcgaaa
     1261 ctcgccggct tgatcgcgga tcaagggggc gaggacaatg acctttatgt cgggtccgta
     1321 agccaggatt tggccgacaa tttgctgcgg cgtttgccgg taaatcggct caccggtttg
     1381 cggatcgtgc ggctggccga cggcggagaa aagcacgcgc aggtaatcgt aaatctccgt
     1441 cgtcgtcgcg attgtcgatc ttgggtttgc gccggccgag cgctgttcga tggcaatcgc
     1501 cggcgataag ccttcgatga aatcgacgtc cggcttttcc atctggtcga ggaattggcg
     1561 ggcgtaggcc gagaggctct cgacgtagcg ccgctgcccc tccgcataca gcgtgtcgaa
     1621 ggcgagcgac gattttcccg agccgctcaa cccggtgatg acgaccagtt tctcccgcgg
     1681 cagctcgacg tgaagattct tcaggttgtg ctggcgcgca ccgctgattt tgatcacctg
     1741 cgcaggcatt cgaccgataa atctcgaacc tttcagtcaa gcacaacccg cactcttctc
     1801 cagcgaaatt gcccgtgcga gacgagcggc catactctcc ccgctaatgt cagccggagc
     1861 agcccgacgc ggtccctcgt ctgcgaatgt gacaatcaaa atggttccca ggcaccccga
     1921 aaacttgact gcttaaagca gccaagcttt caaatcggag gatcggtttt gccggtggcg
     1981 agatcgtgca gttgcgagat cgtgtcggcg gttcgtccgc cacgcacctg cccgccccga
     2041 gagggatcgc acgatcacga ttcccaaatg catccggttg acgcgcggct tcttcccagc
     2101 cgctgttcgc tgcttctgct cttgacacat atacgctaat ccatatatga tatttgatga
     2161 tggatcgaac tcaacccgcc gaaccagcga tgttgttcaa gacactctgc gatccgacgc
     2221 ggctgcgatt gcttaacctg cttgcctgcg gcgagacatg cgtttgtgaa ttgaccgaca
     2281 cgttgcgcgt ggtgcagccg aaagtatcgc gacatctccg gcaccttgaa cgcgccgggc
     2341 tggtggaagc gcggcgaaac ggcaaatgga ttcactatcg ctgggcgcga cacggcgatc
     2401 cgcttgtcca gcatgtgtta atcggtttgc gcgattggat gaaacaagac gcccggatgc
     2461 gacacgagcg ggtgcgccga gaaaaagttt gctgccgagt caaccgaaga aaaccaaagg
     2521 aagtgaaaac gaaatgacga ccgaagaatt tatctccgga ttgcgtcgta cgccggacca
     2581 gccgctgtta tttgtcgatc ttgcgggaca cgttgtgcac cgcggatatc atctcaccga
     2641 gattaaggcc gcatcttttg atacggtcga ttgcggcgga caagtcaatc actggcggga
     2701 gacaattgtg cagctctggg tgccagaaca tgccgaagcg acctacatga ccgctgggaa
     2761 gttcctcaaa atcttcgaga gagtttccgg catggtgccg ctcgacactg aggctgagct
     2821 tcggatcgag tacggtgacg aaaacttttt cccgtcgatt tatcacgtgc gcgcggtcac
     2881 gtatgacggc gatacaacgc gcgtgttgct cgagccaccc tcaatgacct gtaaagcgcg
     2941 cgaccgtcga gttgccagcg cgagcgagcc gtgctgcgaa acaaccgcgg cgtcttgttg
     3001 cgcggcctag cgcgttttaa gatcgacatc tttcgccggc gtttcttcaa tcgcggcgat
     3061 gaa
//
LOCUS       DASTDG010000135         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_101713, whole genome shotgun sequence.
ACCESSION   DASTDG010000135 DASTDG010000000
VERSION     DASTDG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101713"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFO30_04345"
     CDS             complement(<1..225)
                     /locus_tag="VFO30_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET9418550.1"
                     /translation="MIVVICGVAGAGKTTVGQLLAHELGWNFYDGDDFHAAANIDKMR
                     RGIPLTDEDRQSWLTRLRQLIERCLDAGENV"
     gene            282..1655
                     /locus_tag="VFO30_04350"
     CDS             282..1655
                     /locus_tag="VFO30_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET9418551.1"
                     /translation="MHKPEDRSFIGHPRGLGYIAFTEAWERFSYYGMQSLLVLYMVHR
                     LLHRGHIERIAGFVPFRHALETVYRGHLDVQPLASAIFGLYTGLVYLTPIAGGFVADR
                     LLGRTRTITIGALLMAAGHFLMAFDVTFLLALTCLLTGVGCFKGNLASQVGALYSTGD
                     NRRADAFQIYYIFINAGVIISPLIAGTLGEVYGWHWGFGAAGVGMLIGLTIYLSGRKH
                     LPPDSAVVEKSASAAKPRLTRREKLAIVALLVLLPVLTMAIIGNQQIFNAYLVWAERS
                     ADLIFFGKKMPTTWLITLDSIVSVSFLASVIVFWRLWAKKFPEPAEITKIGIGSLVAV
                     TGFISLALGATLAASSGTKVSMGWLVTFHVLNSIGFANIFPVSLALYARVAPAALSAT
                     IIGVYYLAFFAANNLVGTIGGLLEKMPATHFWLLHSALCGIAGIIFLLAGRLFGHLLA
                     PADNESG"
     gene            1731..2258
                     /locus_tag="VFO30_04355"
     CDS             1731..2258
                     /locus_tag="VFO30_04355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9418552.1"
                     /translation="MPKHLVISTSGNPDSNSRRIGRVAFAHLEKRKVDCEWIDIREMD
                     LPLCDADKCYGLPGAKNLSAAIESADGILVAAPVYNYDVAAAAKNMIELSGSAWENKI
                     VGFLCAAGGSASYMSVMSYANSLMLDFRCVIIPRFVFATGEAFQGDEVTDKKVMQRIE
                     QVADELIRFTKALRG"
BASE COUNT          442 a          720 c          620 g          562 t
ORIGIN      
        1 gacgttctcg ccggcatcaa ggcatcgctc gattaactgg cgcagcctcg tcagccacga
       61 ttgtcgatct tcgtccgtca gcggaattcc gcgccgcatc ttgtcgatat tggcggcggc
      121 gtgaaaatcg tcaccgtcgt aaaaattcca cccaagctcg tgtgccagta attgaccgac
      181 ggtcgttttg cccgcgccgg ctacgccgca aatgaccaca atcatctctt cgctgatttg
      241 tctttcctag tttctgcttc ctaccttcta ctttgcagct gatgcacaag cctgaagatc
      301 gcagttttat cgggcacccg cgcggcctcg ggtatatcgc gttcaccgag gcgtgggaac
      361 gcttctctta ttacggcatg caatcgctgc tcgtgctcta catggtgcat cggctgcttc
      421 atcgcgggca cattgagcgc atcgccggct tcgtcccctt tcggcatgcg cttgagaccg
      481 tttatcgcgg tcacctcgat gtccagccgc tcgcctcggc cattttcggt ctctacacag
      541 gccttgttta tctcactcct atcgccggcg gatttgtggc cgatcgtctg ctcgggcgca
      601 cccgcaccat cacgatcggc gcgctgctca tggcggcggg ccattttctc atggcattcg
      661 acgtcacatt cctgctggcg ctcacctgtc tgcttaccgg cgttggttgt tttaaaggaa
      721 acctcgccag tcaggtgggc gctctttact caaccggcga caaccggcgc gccgatgctt
      781 ttcaaatcta ttatatcttc atcaacgccg gcgtgatcat ttcgccgctc attgccggca
      841 cgctcggcga agtttacggc tggcactggg gcttcggcgc cgccggggtc ggcatgctca
      901 ttggcctaac gatttatctc tccggccgca agcatttgcc acccgattcg gcagttgtgg
      961 agaaaagcgc gagtgcggca aaacctcgcc tcacgcgccg cgagaaactg gcaatcgttg
     1021 cattgctcgt tctcttgccc gtgctcacga tggcgatcat cgggaaccag caaatcttca
     1081 acgcctatct cgtatgggcg gagcgcagcg ccgatttaat tttcttcggc aaaaaaatgc
     1141 ccaccacctg gctgatcacg ctcgactcga ttgtgtcggt cagcttcctc gccagtgtga
     1201 ttgtgttctg gcgactgtgg gcgaaaaaat ttcccgaacc ggcagagatc accaagatcg
     1261 gtatcggcag tctcgtcgcc gtgaccggat tcatctcgct cgcgctcggg gcgacgctcg
     1321 ccgcgagttc gggcacgaaa gtgtcaatgg gttggctcgt cacgtttcac gtgctcaaca
     1381 gcatcgggtt cgccaatatc tttcccgtgt cactcgcgct ttatgcgcgc gtcgcgccgg
     1441 ccgcgctgtc tgccaccatc atcggcgtct attatctcgc gttctttgcc gcgaacaatc
     1501 tcgtcggcac aattggcggt cttctcgaga aaatgccggc cacacatttc tggttgctcc
     1561 attccgcgct atgcggcatt gccggaatca ttttcctcct cgccggtcgt ctctttggcc
     1621 atcttctcgc gccggccgac aatgaaagcg gctgattact gagaggtttc ggatcctgac
     1681 ttgattcctg ttttcctgct ttcctaagcg attaaagtct tgcgccagca atgccgaaac
     1741 atcttgtcat cagcacgagt ggtaatcctg acagcaacag tcgccgcatc ggtcgcgtcg
     1801 ctttcgcgca tctcgaaaag cgcaaggtcg actgcgaatg gattgacatc cgtgagatgg
     1861 acctgccact ttgcgacgcc gacaaatgct acggcttgcc cggcgcgaaa aacctctcag
     1921 ccgcgatcga atcggcggat ggaattctcg tggccgcgcc ggtctacaat tacgacgtcg
     1981 ccgcagcggc caagaacatg atcgaactca gcggcagcgc atgggaaaac aaaatcgtcg
     2041 gttttctctg cgcggccggc ggcagcgcca gctatatgtc ggtcatgtcg tacgcgaaca
     2101 gcctgatgct cgattttcgt tgcgtcatca tcccgcggtt cgtctttgcg acgggcgagg
     2161 cgttccaagg agacgaagta accgacaaga aagtcatgca gcgcatcgaa caggtcgcgg
     2221 acgaactgat tcgcttcacc aaggcgctgc gcggctaagc tctccaacct gctcacatga
     2281 aggacggcaa cgacaacatc caacttagtg cagttgaagg tcacgatgga atcttaacca
     2341 ttcg
//
LOCUS       DASTDG010000136         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_534427, whole genome shotgun sequence.
ACCESSION   DASTDG010000136 DASTDG010000000
VERSION     DASTDG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534427"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..733)
                     /gene="pxpB"
                     /locus_tag="VFO30_04360"
     CDS             complement(11..733)
                     /gene="pxpB"
                     /locus_tag="VFO30_04360"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HET9418553.1"
                     /translation="MQITPLGDSALILRVRENFEGAPEETLNAVLRTQRCLEATQLPG
                     VIELAPAYTTVAVFYDPTSAVEAGAPHDDVFGWYERRIRDALKSVARVRDRGPAQPRR
                     ERPVEIPVCYDTEFAPDLNDVARHAGRSTQEVVDLHSGAQYRVHCVGFTPGFPFLGGL
                     DPRVATPRRSTPRKEIAAGSVGIGGAQTGIYPVKSPGGWNIIGRTPLRLFDPQKDPPA
                     LLRAGNRVRFRAITREEFDRLK"
     gene            complement(751..984)
                     /locus_tag="VFO30_04365"
     CDS             complement(751..984)
                     /locus_tag="VFO30_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418554.1"
                     /translation="MFVLTWVGLACWCVCFWWMHRISSRQDTLLKELHEMTARIEQLS
                     KAEHELIREVHPQVGEIKEHVEDVREAVSPDKS"
     gene            complement(992..1570)
                     /locus_tag="VFO30_04370"
     CDS             complement(992..1570)
                     /locus_tag="VFO30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9418555.1"
                     /translation="MRLHILGIAVAIVVLNSVARANETYKFDQARSTIGFAVHQFLGT
                     THGKFTKFDGKIDIDREQPENSSVTARIDVRSLDTGIRRRDDHLCSPEFFNVEKYPDI
                     KFQSRSVKRTGAQSGDIRGDLTMHGVMRPVMLHVELLNSPDDTKATRWAVTTEPIKRR
                     DYNLMFSSGAEAVSGISQTVSVKIEIEAARVQ"
     gene            complement(1567..2142)
                     /gene="recO"
                     /locus_tag="VFO30_04375"
     CDS             complement(1567..2142)
                     /gene="recO"
                     /locus_tag="VFO30_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9418556.1"
                     /translation="MQSTAAILLRKRKFSDTSLIVSWCTESLGCVQTIAKGARRPKTL
                     FAGKLDLFYEAEISIVPSRRSTLHTLAEVMLKNTFVGIRQSYVRTQTAAYFVELIELC
                     TERDHHEPELFSLLRRAFTFLDKNAPTPRAVSHFETELARVAGVHDVTAAKANPAFAL
                     GNLFGRLPLSRTPLLKKLESEARSGGKKILL"
     gene            complement(2272..2592)
                     /locus_tag="VFO30_04380"
     CDS             complement(2272..2592)
                     /locus_tag="VFO30_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418557.1"
                     /translation="MSFALQRTFRRARPSRRWLSPSSLARTSQIALRLFARPHGSLAA
                     FWRRQFHAGAARFRQSDRDRLLWRTRAVFALANVFHFFAHEFTGLRRWRLAFAFVFVR
                     PFDG"
     gene            complement(2789..>3158)
                     /locus_tag="VFO30_04385"
     CDS             complement(2789..>3158)
                     /locus_tag="VFO30_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418558.1"
                     /translation="REIAASGLVDCLAHLDLIKIHGHRPSAEIGSIVVETLELIRARN
                     LAIELSTAGWRKPVNELYPSDQIIQLANVKGIPFTTASDAHSHAQLGDGFDRLAEKMS
                     ISGVREVSVYVKHRRETHSL"
BASE COUNT          656 a          908 c          952 g          642 t
ORIGIN      
        1 ggcggtagcc tcattttaag cgatcgaatt cttcgcgcgt aatggcgcgg aaacgcacgc
       61 gattaccggc acgcagtagc gccggagggt ctttttgcgg atcgaacaat cgcagcgggg
      121 tgcgaccgat gatgttccag cctcccggcg attttaccgg gtaaattcca gtttgcgcgc
      181 cgccgattcc gacggatccg gcggcgattt ctttgcgggg agttgaacgg cgcggtgtgg
      241 ccactctcgg atcgagaccc ccgaggaacg gaaatccggg agtgaacccc acgcagtgga
      301 cgcgatactg agcgccggag tgaagatcga caacttcttg cgttgagcgg ccggcgtgtc
      361 gcgccacgtc gttgagatcg ggcgcaaatt cagtgtcata gcaaaccgga atttcgactg
      421 gacgctcgcg tctaggttga gcaggaccgc ggtcacggac tcgggctaca gatttcaatg
      481 cgtcgcgaat tcgtctttca taccagccaa acacatcgtc gtgtggcgcg ccggcttcga
      541 cggctgaagt gggatcgtaa aagacagcga cggtagtgta agccggcgcc agctcgatca
      601 cacccggcag ctgcgtcgct tccaaacaac gctgggtcct caacaccgcg ttcaaagttt
      661 cttccggcgc accctcgaag ttctcgcgca cacgcaggat gagcgccgaa tctccaagtg
      721 gcgtaatctg cattcggcgg cgcgaagcac tcagcttttg tctggcgaaa ccgcctcgcg
      781 gacgtcttcg acatgctcct tgatttcgcc aacttgcgga tggacttcgc ggatcagctc
      841 gtgttccgct ttggagagtt gttcgatacg ggcggtcatt tcatgtagct ctttgagcag
      901 cgtgtcctgt ctggaagaga tgcgatgcat ccaccaaaaa cagacgcacc agcaggccag
      961 gccgacccac gttaagacaa acacgaccat gtcactgcac ccgcgcggct tcgatttcga
     1021 tctttacgga aacggtttgg ctgataccgg acacggcttc ggcccctgaa ctaaacatta
     1081 gattgtaatc gcggcgcttg atcggctcag tcgtcacggc ccagcgcgtc gccttggtat
     1141 cgtcaggcga attgagcagc tcaacgtgca gcataacggg ccgcatcacg ccatgcatcg
     1201 tgagatcccc gcggatatca ccgctttgcg cgccggtccg ttttacgctg cgactctgga
     1261 acttgatgtc gggatatttc tcgacgttaa aaaactccgg gctgcacagg tggtcgtcgc
     1321 gtctgcggat cccggtgtcg aggctgcgca cgtcaattcg cgcggtgacg gaagaatttt
     1381 ccggctgctc acgatcaatg tcgatcttgc catcgaattt ggtgaatttg ccgtgggtgg
     1441 tgccgaggaa ttgatggacg gcgaagccga tagtcgaacg tgcctggtcg aacttgtaag
     1501 tctcattcgc tctggcgact gagttgagga cgacgatcgc cacggcaatt cccaagatgt
     1561 gcagcctcat agtaatattt tctttccgcc gcttctcgcc tcgctctcga gttttttcaa
     1621 cagcggcgtt cgagacagcg gcaatcttcc gaataggttg ccgagggcga aagcgggatt
     1681 tgccttcgcc gcagttacat catgcacgcc agcgacgcga gccagctcgg tctcgaaatg
     1741 cgaaacggcg cgcggcgtcg gcgcattttt gtcgagaaag gtaaacgcgc ggcgcaagag
     1801 ggagaacaat tccggctcat gatgatcgcg ctccgtgcaa agctcgatta attcgacgaa
     1861 gtaagccgcc gtttgcgttc gtacatagct ttggcggatg ccgacaaacg tgttttttaa
     1921 catcacttcg gccaacgtgt gcagcgtcga tcttcgactg ggcacgatcg aaatctcggc
     1981 ttcataaaaa agatcaagct tgccggcaaa cagcgtttta ggccggcgtg cgcccttggc
     2041 aatcgtctgg acgcagccta acgactcggt acaccaggag acgatcaggc tggtatcgct
     2101 gaatttgcgc ttgcgcagga gaatggcagc agtgctttgc acgccggaac ttagcatatc
     2161 tgctgtagcg gccgctgtcc tcagcggctc tataaatgtc gatcttgcgc agggtggcgg
     2221 ttacatctgg cgccacggtt tggcgcgaac tcaagttgta tgccagagga atcagccatc
     2281 gaacgggcgc acgaagacga acgcgaaggc aaggcgccat cgacgcaagc cggtgaattc
     2341 gtgcgcgaag aaatggaaca cattcgcgag ggcgaacacg gcgcgcgttc gccacagcag
     2401 gcgatcgcga tcggattgtc gaaagcgcgc cgcgccggcg tgaaactgcc gccgccaaaa
     2461 cgcggccaag ctcccgtgcg gacgcgcaaa caagcgcagc gcgatctgcg acgtgcgcgc
     2521 aagcgaactc ggcgaaagcc atcgccgcga cggtcgcgcg cgacgaaacg tgcgctgcag
     2581 cgcgaaggac actccgccgc atcacgcaaa gctctgtccc ggcaggcgcg ttctgcagcg
     2641 cgccggcgct ctacccgttc gcgttctgct gccgctcaaa aagcggcggg cacgaggaag
     2701 cggcgtcgct aagcgtgttg tgtagccaac ggcctatggc cggtcaggga aaatgaagcc
     2761 gcaaactcga cggctcacag agccgtcgct acaggctgtg cgtctcgcgc ctgtgtttca
     2821 cgtaaacgct gacctcgcgc acacccgaga tcgacatctt ctcggcgagc cggtcaaagc
     2881 cgtcgccaag ctgcgcgtgt gagtgcgcat cggacgcagt tgtgaatgga atgcctttta
     2941 cgtttgccaa ctgaatgatc tgatcgctcg ggtaaagttc gttcaccggt ttgcgccagc
     3001 cggcagtcga aagttcgatc gcgagattgc gtgcgcggat gagctcgagt gtttccacca
     3061 caatcgagcc aatctccgcg ctgggtcggt gcccgtggat tttgataaga tcgaggtggg
     3121 cgagacaatc gacgaggccg ctcgccgcga tctcgcga
//
LOCUS       DASTDG010000137         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1012591, whole genome shotgun sequence.
ACCESSION   DASTDG010000137 DASTDG010000000
VERSION     DASTDG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012591"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFO30_04390"
     CDS             complement(<1..357)
                     /locus_tag="VFO30_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET9418559.1"
                     /translation="MTLQQTNLRTGSTVSDRAAAVAAAVELLRAGEVVALPTETVYGL
                     AADALNPIAVAKIFEAKERPRFDPLIVHLPEGEWLERVAQPLRQDRQLLAQLTERFWP
                     GPFTVVLPKRQVIPDLV"
     gene            complement(467..964)
                     /gene="purE"
                     /locus_tag="VFO30_04395"
     CDS             complement(467..964)
                     /gene="purE"
                     /locus_tag="VFO30_04395"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9418560.1"
                     /translation="MAETKTNSLVAILMGSTSDWETMSACVKTLDELGIANEAHVLSA
                     HRTPDATADFARKAAGRGIRVIIAAAGGAAHLAGVIAAHTWLPVLGVPMKSALNGLDS
                     LLSIAQMPGGIPVGTLAIGSGGAKNAALLAAAILATSDNTIRKTLEAFRAKQTETVLA
                     AKLPP"
     gene            1026..1559
                     /locus_tag="VFO30_04400"
     CDS             1026..1559
                     /locus_tag="VFO30_04400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9418561.1"
                     /translation="MDQPGKSIVLIGMMGAGKSSVGRCLQRRTGADLFDTDDAVTKKL
                     GLSIVEIFKRFGEEKFRHAETEVLRELAPTKPTIFVTGGGAILREENVNLLKRLGFVV
                     WLDADEGTLCERATRRGHRPLLQTENPRARLAALVAERNPLYARAADLRIDTSTMDHD
                     EVADIVLSKVQELFATR"
     gene            1556..>2513
                     /gene="queG"
                     /locus_tag="VFO30_04405"
     CDS             1556..>2513
                     /gene="queG"
                     /locus_tag="VFO30_04405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9418562.1"
                     /translation="MNATADSALRSGSEDVAPASSSATIKARLVAFAREVGFDLCRVV
                     RCESPLHAREFRQWLRDGAHGDMSYMQRGEERRCDPQLVLPGVKSVVVLALNYFPGDP
                     PQSGATTGRVARYAWGDDYHELIAAKLDKIDNFLREFGGRQKCYVDTGPVLERDFAAQ
                     AGIGWHGKSTMLINERLGTWFFLAEILTTLELPPDEPARDRCGSCHRCITACPTGAIT
                     APYRLDARRCISYLTIELKGAIPLELRLLIGDRIFGCDDCLAACPWNRFAQVARESAF
                     TARRSTIDFALRDYLALDEPRFRSIFRNSPIKRIKRRGFLRNV"
BASE COUNT          504 a          713 c          774 g          522 t
ORIGIN      
        1 gacaagatcc gggattactt gacgctttgg caaaacaacc gtgaacggcc cgggccagaa
       61 tctctccgtt agttgcgcga gcaattgtcg atcttgccgc agcggttggg cgacgcgctc
      121 gagccactcg ccctctggca agtgcacaat gagcgggtca aagcgcggcc gctccttcgc
      181 ctcaaaaatc ttggcgaccg cgattggatt cagcgcgtcg gccgcgagtc cataaaccgt
      241 ttcggttgga agcgcgacga cttcaccggc acgcagcaat tcaacagccg cggcaaccgc
      301 tgctgcgcga tcacttaccg ttgagcctgt acggagatta gtttgctgca gtgtcattcc
      361 gagcgcagcg aggaacctcc catcaacggg ggaagatcac gctgtttggc ttgtgtgatc
      421 aaggtctcat aggtgagatc cttcgctccg ctcaggatga ccacgctcag ggcggcaact
      481 tcgctgcgag cactgtctcc gtctgcttcg cgcgaaatgc ttcgagcgtt ttgcgaattg
      541 tgttatccga cgtcgcgaga attgccgcgg cgagaagagc ggcgtttttc gcaccgcctg
      601 aaccgatggc aagcgttccc accggaattc cgcccggcat ctgcgcgatt gacaaaagcg
      661 aatccaaacc attgagcgcg gatttcatcg gcacgccgag cactggcagc cacgtatgcg
      721 ctgcgatgac cccggcgaga tgcgcagcac cgcccgccgc agcgatgatg acgcgaatgc
      781 cgcgaccagc agctttgcgc gcgaaatcgg cggtcgcatc cggtgtgcga tgagccgaga
      841 gcacgtgtgc ttcgtttgca atgccgagct cgtccagagt tttcacgcag gcggacatcg
      901 tttcccaatc cgaagtgctg cccatgagga tcgcgactaa cgagttagtt ttcgtctcgg
      961 ccatggctgg agtgtaacca cgatgtcgat cttttcattg tcgatcttac gccgatattg
     1021 gcggcgtgga tcagccaggc aaatcgattg tcctgattgg aatgatgggc gccgggaaat
     1081 cgtccgtcgg gcgatgtctt cagcgccgta ctggtgctga tctattcgac accgacgatg
     1141 cggtgacgaa aaaacttggc ctgagcattg tcgaaatctt caaacgattc ggggaagaga
     1201 aatttcgcca cgccgaaacg gaagtgcttc gcgagcttgc accgacgaag ccgacgatct
     1261 ttgtaacggg cggcggagcc attttgcgcg aggaaaacgt gaatcttttg aagcggctcg
     1321 ggttcgtcgt gtggctggat gccgatgaag ggacgctttg cgaacgtgcc acccgccgtg
     1381 gacatcggcc gttattgcag acggaaaatc cacgagcgcg gctcgcggca ctcgtggccg
     1441 agcgcaatcc gctctacgcg cgcgcggccg acctgcggat cgacacttcc acgatggacc
     1501 acgacgaagt cgccgacatc gttctcagta aagttcagga actattcgcc acgcgatgaa
     1561 tgcaactgcg gactccgcgc tgcgcagcgg cagtgaagat gtggcgccgg ccagctcgtc
     1621 cgccacgatt aaggcgcggc tagttgcctt cgcgcgcgaa gttggatttg atctgtgtcg
     1681 cgtggtgcga tgcgaatccc cactgcacgc gcgcgagttt cgccaatggc tgcgtgatgg
     1741 tgcgcacggc gacatgagtt acatgcaacg cggcgaagaa aggcgttgcg atccgcagtt
     1801 ggtgctgccc ggcgtgaaat cggtcgtcgt actcgcgcta aattactttc caggtgatcc
     1861 gccgcagagc ggcgcgacta caggaagggt cgcgcggtat gcgtggggcg acgattacca
     1921 tgaactgatt gccgcgaagt tggacaagat cgacaatttc ctgcgcgaat tcggcggtcg
     1981 ccaaaaatgt tatgtcgaca ccggcccggt gttagagcgt gattttgccg cgcaagcggg
     2041 aatcggctgg catggcaaga gcacgatgct gatcaacgag cgactcggaa cgtggttttt
     2101 tcttgcggag attctaacga cgcttgagct gccgccggat gagccggcgc gcgatcgttg
     2161 cgggagctgt caccgctgta tcactgcttg cccgaccggc gcgatcacgg cgccatacag
     2221 attggacgca cggcgctgca tctcgtacct gacgattgaa ctcaaaggcg cgattccgct
     2281 cgagcttcgc ctgctaatcg gagaccgcat ttttggttgc gatgactgtc tcgcggcgtg
     2341 tccatggaat cggtttgcac aggtggcccg ggagagcgcg ttcaccgcgc gaagatcgac
     2401 gatcgatttc gcgctgcgcg attacctggc gctcgacgag ccgcggtttc gcagcatttt
     2461 tcgtaattcg ccgatcaaac gaattaagcg ccgcggattt ttgcggaacg tgt
//
LOCUS       DASTDG010000138         5477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1493319, whole genome shotgun sequence.
ACCESSION   DASTDG010000138 DASTDG010000000
VERSION     DASTDG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493319"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFO30_04410"
     CDS             <1..988
                     /locus_tag="VFO30_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9418563.1"
                     /translation="GATMMVYDGAPDFPGPDRVWKLCADHKVTALGISPTLIRALRRH
                     GDDMVHRHDLSSLRKFASTGEPWNPDPWMWLFQNVGRGQLPIINYSGGTEISGGIVMG
                     NVLTSMKPCAFSGPLPGMAADVVDENGNSVRGRVGELVIREPWIGMTRGFWKDRQRYI
                     DTYWSRFPDVWVHGDWAAVDDDGLWYILGRSDDTIKIAGKRLGPAEVESVLVAHPQVS
                     EAAAVGVPDSIKGEALVCFCVLKKDAHGDVDSSTSLRTNLASELKQRVADSLGKALAP
                     KEVLFVGDIPKTRNAKVMRRIVRAAYLGEKPGDVSALENPNAVAEITRAAKK"
     gene            complement(1041..1466)
                     /locus_tag="VFO30_04415"
     CDS             complement(1041..1466)
                     /locus_tag="VFO30_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418564.1"
                     /translation="MSDTPATFFITKSLRPKKPVLDDSARETIVSALGFAVEHKRIFL
                     RAFVVMPDALVALRDLWTLPKLTHHLMSYVGARTPTLLQSHKTCWQDGYYDTRVKTAK
                     QFEFVAYYIEQNPVVKGLVESSDQWDASSAARRHLLTDP"
     gene            complement(1541..3379)
                     /locus_tag="VFO30_04420"
     CDS             complement(1541..3379)
                     /locus_tag="VFO30_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9418565.1"
                     /translation="MSKLGAVVESLEKHQQFVANQVKRARSDEKFARELTERWNKLKA
                     ETPTTRTPTGLNLPRLALPEIDEPGEIARYLFGDGLPGEFPYLNAAYREMYLEPLLTN
                     GAIETGSYGNKPSPERSTSGNDAKKNGQRPLEEPTRLFSGLMLAEDTNKRFHFLSAHQ
                     RSKRLSTAFDGPTLYGIDSDSDGVFGKIGEGGVAVDTVEDMMRLYDGFDLGSPDFSAS
                     MTISGPAPMIMAMYIAAAKRRFGPKVVPNLRGTIQADIFKEVQAQNETIFPVEASLRF
                     LCDMIEWTSTRMPRWYPISISGYHIAEAGSTPVQQAAYTLSNGFAYVEMLTARGVPVD
                     RFGPRLSFFLDCGLDVEYVALARVSRKIWAIGMRDVFGAGPKAQLFKLHTQTSGRSLI
                     AAEFKNNLTRTAAELMLAYINCTNSCHSNSADEPFTTPSEEWIRLAAHGQAILLEESG
                     IFKHTMNMLSGAPGMKALEHVLEDAILDEFREIERLGGVLAAVENRYQRSQIQNAAHR
                     YEEQIYDGTRPIIALNRYRNGSDEMPELELVRTPRSKQQQQVNRLKKFKQRNAAKAKK
                     ALDKLAAVVETDQNCFPALLEAAEVCSLGQITGRLQEVVGRFRPMV"
     gene            complement(3321..4541)
                     /locus_tag="VFO30_04425"
     CDS             complement(3321..4541)
                     /locus_tag="VFO30_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9418566.1"
                     /translation="MPSPVRILTAVPICDGHDSAINTINLEFIRHGLEVVYLGFHRYV
                     SDIVRAAIQEDVAAVGLSSYNGGHIEFFAEVVAQLRKKGAADIAVFGGGGGTITHDDA
                     RVMKRRGVDQIFFAGTPLSDMVAHVKKHYGKARNRRALSNPDLVLARKLTEIEEARGK
                     TRNRQSATRNSKRARVIGFTGPGGAGKTTLIDELVLRFLTRDRSAAGRNRRGNPSRIA
                     ILSHDPSVMGDGALLGDRATMINSQDDRVFMRSMATRGQAGGLSPATESCLQLLARAG
                     FDYVIIETVGTGQEAMPFRNQLVDQTVLVMNPDYGARLQLQKIVMLDVADIVVVNKTD
                     LYRAKAALAEIEQRLTQNKRDQQMVATVAKRHRDAGVDELFALITQRNRKRGHDEQTR
                     SSRRITRKAPAVRR"
     gene            4593..5348
                     /locus_tag="VFO30_04430"
     CDS             4593..5348
                     /locus_tag="VFO30_04430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9418567.1"
                     /translation="MSDPLDFSGKVVLVTGSSRGIGAEMIKAFGKRGAACVVNYVADE
                     SGQNETDATKVAKELNDPLVIECDVAEPARVEVMMKKIQDRHGDLDILVNNSGIIADR
                     SIKKMSLAQFESVLRVNLVGTFNVTQKAAAILRPGGRIVNLSSVSGQMGLFGQANYSS
                     SKAAIIALTKVSAREFARQNITVNAIAPGFIDIGMSKGMPDKVTENFIKQIPLGRLGN
                     VREIVDAALFLASPMASYITGHVLNVNGGFYMG"
BASE COUNT         1104 a         1555 c         1632 g         1186 t
ORIGIN      
        1 cggtgccacc atgatggtct acgacggtgc gcccgatttt cccggaccgg atcgcgtctg
       61 gaaactttgc gcggatcaca aagtgaccgc actcggcatc tccccgacgc tgattcgcgc
      121 gctgcgacgt cacggcgacg atatggtgca tcgccatgac ctctcgtcat tgcgcaagtt
      181 cgcttcgacg ggcgagccgt ggaatccaga tccgtggatg tggctgtttc aaaatgtcgg
      241 tcgcggccaa ttgccgatca tcaattactc cggcgggacc gaaatctccg gcggcatcgt
      301 gatgggcaac gtgctgacgt cgatgaagcc atgcgcgttc tccggcccgc tgccgggaat
      361 ggcggcggac gttgtggacg aaaacggaaa ttcagtgcgc ggtcgggtcg gcgagctggt
      421 gatccgcgag ccgtggatcg gtatgacacg cggtttctgg aaagatcgac aacgctacat
      481 cgacacttac tggtcgcgtt ttcccgacgt ctgggtgcac ggcgactggg cggcggttga
      541 tgacgatggc ctctggtaca ttctcggccg cagcgacgat acgatcaaaa tcgccggcaa
      601 acgcttgggt ccggccgagg ttgaatccgt tctagtcgcg cacccacaag tgagtgaggc
      661 cgcggccgtt ggtgtgccgg attcgatcaa aggcgaagcg ctcgtctgct tttgcgtatt
      721 gaaaaaagac gcgcatggag atgtcgattc ttcgacctcg ctcaggacaa atcttgcgag
      781 cgagttgaag cagcgagttg ccgattcgtt aggcaaagcg cttgcaccaa aagaagttct
      841 cttcgtcggc gacatcccaa aaacgcgcaa cgccaaagtc atgcggcgga tcgtgcgcgc
      901 ggcatatctc ggcgaaaaac ccggtgacgt atccgcgctc gaaaacccga atgccgtcgc
      961 agagatcaca cgcgcagcga agaaataatg tgggaggggc ctgagtgccc cgactgatta
     1021 aatcacgcgt acagccaagg tcatggatcg gtgagcaggt gacggcgggc tgcactgctc
     1081 gcatcccact gatcagatga ttcaacgagt ccttttacca ctggattttg ctcgatgtaa
     1141 taggcgacga attcgaactg cttggctgtt tttacccgcg tatcgtagta gccgtcctgc
     1201 cagcaagttt tgtgagattg aagaagcgtt ggtgttcttg ccccgacata actcattagg
     1261 tgatgcgtga gttttggtag tgtccataag tcgcgcaatg caaccagtgc gtctggcatc
     1321 actacaaagg ctcgaagaaa gattcgcttg tgttcaacgg caaaaccaag tgccgagaca
     1381 atcgtttcgc gcgcactgtc gtcgaggacc ggcttctttg gccgaagtga tttggtgata
     1441 aaaaatgtcg ccggtgtatc gctcaaccgg tgcaaccgca ggttgcgcga atgagcctgc
     1501 gacctcatga ggagtcgggg cactgaggcg cctcccacat tcacaccatc ggccggaaac
     1561 gccccaccac ttcctgcaaa cggccggtga tttggcctaa cgaacagact tccgccgctt
     1621 cgagcagcgc cgggaaacag ttctggtcgg tttcaacgac ggcggcgagt ttgtcgagcg
     1681 ccttcttggc tttggccgcg ttgcgctgtt tgaatttctt caagcggttt acctgctgct
     1741 gctgcttcga tcgcggtgtg cgcaccagct cgagctcggg catttcatcg gaaccattac
     1801 ggtaacggtt gagcgcgatg atcgggcgcg tgccatcgta gatctgctcc tcatagcgat
     1861 gcgcggcatt ctggatctgg ctgcgttgat agcggttctc cacggcggcc aacaccccgc
     1921 cgagtcgctc gatttcgcgg aattcatcga ggatcgcgtc ctcaagcacg tgttcgagcg
     1981 ctttcattcc cggcgcgccg ctaagcatgt tcatggtgtg cttaaagatg cccgactctt
     2041 cgagcagaat ggcctggccg tgcgccgcga ggcggatcca ttcttcactc ggagtggtga
     2101 acggttcgtc ggcgctgttc gagtggcatg agttggtgca gttgatgtag gcgagcatca
     2161 gctcggccgc ggtgcgggtg agattgtttt tgaactcggc cgcgatgagc gatcgtccgc
     2221 tcgtctgggt gtgcagcttg aacaactgtg ctttcggccc ggcgccgaaa acatcgcgca
     2281 tcccgatcgc ccaaatcttt cgcgaaacgc gcgcgagcgc gacgtactcg acatcgagcc
     2341 cgcaatcgag aaagaacgaa agtcgcggac cgaatcgatc gactggcacg ccgcgcgcgg
     2401 tgagcatctc gacgtaagcg aagccgttcg aaagcgtata agcggcctgc tgcacgggcg
     2461 tagagccggc ttcggcgatg tgataaccgc tgatcgagat cggataccag cgcggcatgc
     2521 gcgtgctcgt ccattcgatc atgtcgcaaa ggaaacgcag cgacgcttcg actggaaaaa
     2581 tcgtttcgtt ctgcgcctga acttccttga agatgtcggc ttggatggtg ccgcgtaggt
     2641 tcggcacgac tttgggcccg aaccgccgct tggccgcggc gatgtacatc gccataatca
     2701 tcggtgccgg accgctgatc gtcatcgaag cggaaaaatc cggcgacccc agatcgaacc
     2761 cgtcgtaaag acgcatcatg tcctcgacgg tgtcgaccgc caccccgccc tcaccgattt
     2821 ttccgaacac gccatcggaa tcggaatcga tgccgtaaag cgtcggccca tcgaaggcgg
     2881 tgctgaggcg tttcgaccgc tggtgcgcgc tcaggaaatg gaaccgtttg ttcgtgtcct
     2941 cggcgagcat gagcccggag aacaggcgcg tgggttcttc cagtggtcgc tggccgtttt
     3001 tctttgcgtc gttgccggat gtcgatcttt caggtgatgg cttgttccca tagctgcccg
     3061 tttcaatcgc gccgttggtc agcaacggct caaggtacat ctcacgatag gcagcattga
     3121 gatatggaaa ctcgcccggc aacccatcgc caaagaggta gcgcgcgatc tcgccgggct
     3181 catcgatctc cggcagggcg agccgcggca ggtttaagcc cgtcggcgtc cgcgtcgtcg
     3241 gcgtttctgc tttgagcttg ttccagcgct cggtcagctc gcgcgcaaat ttctcgtcac
     3301 tccgggcgcg tttaacctga ttagcgacga actgctggtg cttttcgagt gattctacga
     3361 ctgctccgag tttgctcatc gtgtcctcgc tttctgtttc gttgagtaat taaggcgaac
     3421 aattcatcca cgccagcatc gcggtgtcgc ttggccacgg tcgcgaccat ttgctgatcg
     3481 cgcttgttct gggtgagccg ctgctcgatt tcggccaggg ccgctttcgc gcgatagaga
     3541 tcggttttgt taacaacaac gatatcggcg acatcgagca tgacgatttt ctgcaattgc
     3601 aagcgcgcgc cgtaatccgg gttcatcacc agcaccgttt gatcgacaag ctgatttcgg
     3661 aacggcatcg cttcctgccc ggtgccgacg gtttcgatga tcacgtagtc gaacccggca
     3721 cgcgcgagca gttgcagaca actttccgtt gccggggaca aaccgccggc ctgtccgcgc
     3781 gtcgccatgc tgcgcataaa tacgcggtcg tcctgcgaat tgatcatggt cgcgcggtcg
     3841 cccagcaaag cgccgtcacc catcacgctt ggatcgtgcg aaagaatggc gatgcgagac
     3901 gggttgcccc gtcgattccg tccggcggcg gatcggtctc gtgtcagaaa gcggagcacg
     3961 agctcgtcaa ttaacgtcgt cttacccgct ccaccgggac cggtgaatcc gatcacgcgc
     4021 gcgcgtttcg aattgcgcgt ggccgattgg cgattgcggg tttttcctct ggcctcttcg
     4081 atctcagtca atttgcgcgc cagcacgaga tcaggatttg agagcgcgcg ccggtttcgc
     4141 gctttcccgt aatgtttctt cacatgcgcg accatgtcgc tgagcggggt gccggcgaag
     4201 aagatttgat cgacgcctcg gcgcttcatc acacgcgcgt catcgtgcgt gatggtgccg
     4261 ccgccgccgc cgaacacggc aatgtcagcc gcgccttttt tgcgaagctg cgcaacgact
     4321 tcggcgaaaa actcgatgtg gccgccgttg taactcgaaa ggccgactgc cgcgacgtct
     4381 tcctggatgg cggcgcgcac gatgtcgctg acgtagcggt gaaagccgag atacaccacc
     4441 tcgagcccgt gccgaatgaa ttcgagatta atcgtattga tcgcgctgtc gtgcccgtca
     4501 caaatcggca cagcggtgag gatgcggacc ggtgaaggca tcagggaaga ttgtcgttga
     4561 atcgttaaat cggtaaatcg ttaaatcgtc aaatgtcgga cccgttagat ttcagtggca
     4621 aagtcgtgct cgttacgggc agctcgcgcg ggatcggcgc ggaaatgata aaggcgtttg
     4681 gcaaacgcgg cgcagcctgc gtcgtcaatt atgtggcaga cgaatcgggc caaaatgaga
     4741 cggatgcgac gaaggtggcg aaggaactca acgatcccct tgtcatcgag tgtgatgtcg
     4801 cagagccagc gcgagtcgag gtcatgatga aaaaaataca agatcgacat ggggacctgg
     4861 acattctcgt caataactcc ggcattattg ccgaccgctc gatcaagaag atgtcgcttg
     4921 cgcagttcga gagtgtcctg cgcgtaaacc tggttggcac gttcaacgtg acccaaaaag
     4981 ccgccgccat tctgcgcccc ggcggacgga tcgtgaatct ttcctcggtc agcggccaaa
     5041 tgggattgtt cggccaggcg aattattcct cgagcaaggc ggccatcatc gcgctgacga
     5101 aagtttcggc ccgcgagttt gcgcgtcaaa acattaccgt gaacgcgatc gcaccaggat
     5161 tcatcgacat cggcatgagc aaaggcatgc ccgataaagt gacggaaaat ttcatcaaac
     5221 aaatcccgct cggccgactt ggtaacgttc gcgagatcgt tgacgcggcg ctcttcctcg
     5281 cctcgcccat ggcctcatat atcaccggcc atgtcctaaa cgtgaacggc ggcttttaca
     5341 tgggctgagc tgcggctgga tgattcgcgt gctcgatacg cgccaacttg gacgcgccgg
     5401 tattgttgcg gcgacagcgc ttgagaccga cgacggaatc gcattgttcg acaccggacc
     5461 ggaatcaact ttcacca
//
LOCUS       DASTDG010000139         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_173142, whole genome shotgun sequence.
ACCESSION   DASTDG010000139 DASTDG010000000
VERSION     DASTDG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173142"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFO30_04435"
     CDS             <1..389
                     /locus_tag="VFO30_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418568.1"
                     /translation="ADIFLREARFALILRKLLAQQGISHVHATSSRTLVCALLLQQLT
                     NLSVSAAIEAHPQLSRNWLRSALQRCVGGRIADRKLLAQTSDSFLPEARTRFGLTGRA
                     KFWQQWAELVERWSCANRRSTIESRK"
     gene            386..1279
                     /gene="rfbA"
                     /locus_tag="VFO30_04440"
     CDS             386..1279
                     /gene="rfbA"
                     /locus_tag="VFO30_04440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET9418569.1"
                     /translation="MKKAILLAGGAGTRLFPLTKIVCKQLLPIYDKPMICYPLATLML
                     GGLREVLIISTPKDVPMLRNYLGDGAQLGIRIEYAEQPKPEGIAQAFLIGAKFIDDSG
                     ASLILGDNIFYGKLDFYREALALERGACIFAYQVRDPQRYGVVEFGMDGKAVSLEEKP
                     KQPKSQFAVPGLYVYDAQVVDLCRTLRPSARDELEITDLNLLYLERTQLHVKLLSRGV
                     AWLDTGTTTTLLEASNYIATVEHRQGLKIACLEEIALRMGFITQSDLTRIIDGLPKNE
                     YRDYLRMVCEEGPSRRPEPVG"
     gene            complement(1336..1986)
                     /locus_tag="VFO30_04445"
     CDS             complement(1336..1986)
                     /locus_tag="VFO30_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9418570.1"
                     /translation="MNATTVAESGATWRATAWKVTLLAMPLLTLVILLWLRDVEVKAL
                     RQRTVSTYGTVPPFQLTNQNGQPFGSADLQGKIWIADFIYTTCPGPCPMISSRMSELQ
                     QPLEKTGVHLVSFTVDPVKDTPEVLRGYAAKLHAQPGRWDFLTGPQSTIYDISRNGFK
                     LAALDRSEATGLPVHSTRMILVDRHGAIRGYYDATEPDAMTKLLADTNHLLREQPQ"
     gene            complement(1983..2879)
                     /gene="cyoE"
                     /locus_tag="VFO30_04450"
     CDS             complement(1983..2879)
                     /gene="cyoE"
                     /locus_tag="VFO30_04450"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9418571.1"
                     /translation="MKTPAMTPTAALARSRLLADFAELVKARLTLLVLLTTAVGYYLG
                     APSPIDYTGLFHVVFGTAAAAAGAAALNEWWERRADALMQRTRLRPVPAGRMRAADAL
                     VLGTGLSIFGVAYLVIFCNGLSAVLAAITIAIYIFAYTPLKRISTGNTALGAVPGALP
                     PVIGWAAARGTVDAGACSLFVIVFLWQLPHFFAIAWMCREDYARAGFRMISSEDQSGA
                     RSASQSVFFCILLLVMAGLPAFVEVTRAVYLPIELALSGGYLAAAMRFLRTQRVADAR
                     RLFIASIVYLPLLLGTLVLTKA"
     gene            complement(2876..>3456)
                     /locus_tag="VFO30_04455"
     CDS             complement(2876..>3456)
                     /locus_tag="VFO30_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9418572.1"
                     /translation="TKLWRSVSNTVVDRASTKRLQTLAMAITVAIYVQLALGAIMRHQ
                     HRDLAILDFPTANGAWIPDTSPAALAKINPWRDMHGFSDVSAFQIWLQMAHRFLAVLI
                     AIGVIAFCLRVWRDTRDCLVLQRLSMWWVIGIFVQLTLGAWTIWSNKAADIATAHVAV
                     GAVMLCFGVSLWAICWRLCQPSRRTEPQRVLV"
BASE COUNT          761 a         1047 c          976 g          672 t
ORIGIN      
        1 ctgccgacat tttcctccgc gaagcgcgct tcgcccttat tctgcgaaag ctgctcgccc
       61 agcaaggcat ctcacatgtt cacgccacaa gctcgcgcac cctcgtctgc gcgctgctct
      121 tgcagcaact taccaatctt agcgtcagcg cggcgatcga agcgcacccg caactctcgc
      181 gtaattggct ccgaagcgca ctgcagcgat gcgtcggcgg ccgtatcgct gatcgtaagt
      241 tgctcgcgca aacgagtgac tcgtttctgc ctgaagcaag aacgcggttc ggtttgaccg
      301 gacgcgcgaa attttggcaa caatgggcgg aattagttga gcgttggagc tgcgctaatc
      361 gaagatcgac aatcgaaagt cgaaaatgaa aaaagccatt cttctcgccg gcggcgccgg
      421 gacgcgtctc tttccgctga caaagattgt ctgcaaacaa ctgctgccga tttacgacaa
      481 gccgatgatc tgttacccgc tcgccacact catgctcggc ggcttgcgcg aggtgctcat
      541 catctccacg cccaaggacg tgccgatgct ccgcaattat ctcggagatg gcgcgcagct
      601 cggaattcgc attgaatatg ccgagcaacc aaaacctgaa ggcatcgcgc aagccttttt
      661 gatcggcgcg aagtttatcg atgactccgg cgcgtcgctc attttgggag acaatatttt
      721 ttacggtaaa ctcgatttct atcgcgaggc gctcgcgctc gaacgcggcg catgcatctt
      781 cgcttatcag gtccgcgacc cgcagcgcta tggcgtggtg gaattcggca tggacggcaa
      841 agcagtgagt ctcgaagaaa aaccgaagca gccgaagagc cagttcgccg tgccgggtct
      901 ttacgtttat gacgctcagg tggtcgatct ctgccgcacg ttaaggccat cggcgcgcga
      961 cgagctcgag atcaccgatt tgaatttact ttatctggag cggactcagc tccacgtgaa
     1021 attgctcagc cgcggcgtcg cctggcttga taccggcaca accacgacgc tgcttgaagc
     1081 gagcaattac atcgcgacgg tcgagcaccg gcaggggctc aaaattgcat gcctcgaaga
     1141 aatcgctctg cgcatgggct tcatcacgca atcagacctg acgcggatca ttgacggtct
     1201 gccgaagaac gaatatcgcg attatctgcg catggtctgc gaagaaggcc cgtcgagacg
     1261 ccccgagcca gtcggctgat tttgtagccg cttcgctacg cgaagcgctc gtcgcgcaca
     1321 gcgcgaccac tacagctact gcggttgttc gcgcagcaaa tgattggtgt ccgcgagcag
     1381 ctttgtcatc gcgtccggct cggtcgcgtc gtagtagccg cggatcgcac catgtcgatc
     1441 taccaaaatc atccgcgtgc tgtgcaccgg cagtcccgtt gcttcgctgc ggtcgagcgc
     1501 ggccagctta aagccgtttc gtgatatgtc gtaaatggtc gattgtggtc cagtgagaaa
     1561 atcccagcgc cccggttgcg cgtggagttt tgcggcgtaa ccacgcagca cttcgggcgt
     1621 gtctttcacc ggatcgaccg tgaacgaaac gaggtggaca ccggttttct cgagcggttg
     1681 ctgcaattca ctcattcgcg agctaatcat cgggcacggc ccggggcatg tcgtgtaaat
     1741 gaaatctgcg atccagattt tgccctgcaa atcggccgac ccgaacggct ggccgttttg
     1801 attggtgagt tgaaacggcg gcactgttcc ataggtagaa acggtgcgtt ggcgcaacgc
     1861 cttcacctcc acgtcgcgca gccacagcaa tatcactaaa gtaagcagcg gcatcgcgag
     1921 tagcgtcacc ttccatgctg ttgcgcgcca agtcgcgcca ctctcggcta ctgtcgttgc
     1981 attcatgctt tcgtcagcac gagggtgcca agcagcagcg gcaaataaac aattgacgcg
     2041 atgaacaaac gtcgcgcgtc ggcgacccgc tgcgtccgga gaaagcgcat tgctgctgcc
     2101 agatatccac cgctgagtgc aagctcgatc ggcagataca ctgcgcgcgt cacttcaacg
     2161 aaggccggca gtcccgccat aaccagcagc aaaatgcaaa agaagacgct ttggctcgca
     2221 ctgcgagcgc cgctttggtc ctcgctcgag atcatgcgaa acccggcacg cgcgtaatcc
     2281 tcccggcaca tccaggcgat ggcgaagaag tgcggcagtt gccacaggaa cacgatcaca
     2341 aacaggctgc acgcgccggc gtcgaccgtg ccgcgcgccg ccgcccagcc gatcaccggc
     2401 ggaagcgctc ccggcacagc accgagcgca gtgttacccg tgctgatgcg tttcagcggc
     2461 gtgtaagcaa agatgtaaat ggcgatcgtg atcgcggcga gcaccgcgct cagcccgttg
     2521 cagaaaataa cgagataagc gacgccgaag atcgacaatc cagttccgag aaccagcgca
     2581 tcggccgcgc gcattcggcc cgcaggcacc gggcgcagtc tggtgcgctg cattagcgca
     2641 tcggcacggc gttcccacca ctcgttcaac gctgctgcac ctgccgcggc ggccgcggta
     2701 ccaaatacaa catgaaagag tccagtataa tcaattggac ttggcgcgcc tagatagtag
     2761 ccgacagcag tggttaaaag cacaagcagc gtcagccgcg ctttaaccag ctcggcgaaa
     2821 tctgcgagga gtcggctgcg cgccagagcc gcggtaggag tcattgccgg cgttttcata
     2881 cgagcacgcg ctgcggctca gtccgtcggc tcggctggca tagccgccag caaattgccc
     2941 agagactcac accgaaacat aacatgaccg cgcccaccgc cacatgcgcc gtcgctatgt
     3001 ccgcggcttt gttactccaa atcgtccacg cgcccagcgt gagttgcacg aaaatcccga
     3061 tgacccacca catcgacaat cgctggagca cgagacaatc ccgtgtgtct cgccacaccc
     3121 gcaaacaaaa agcgatcacg ccgattgcga tcagcacagc cagaaaccga tgcgccattt
     3181 gcagccaaat ctgaaacgcg ctcacatcgg aaaacccatg catgtcgcgc cagggattga
     3241 tctttgccag agcggcaggg ctcgtgtcgg gaatccacgc gccgttcgcg gtcggaaaat
     3301 cgaggattgc caggtcgcga tgctggtgtc gcatgattgc gccgagcgcg agctgcacgt
     3361 aaatggcaac tgtaattgcc attgccagag tttgaaggcg tttggtgctt gcacgatcga
     3421 caacggtatt tgaaaccgag cgccaaagct tggttg
//
LOCUS       DASTDG010000140         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1569128, whole genome shotgun sequence.
ACCESSION   DASTDG010000140 DASTDG010000000
VERSION     DASTDG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569128"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFO30_04460"
     CDS             complement(<1..187)
                     /locus_tag="VFO30_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418573.1"
                     /translation="MARKLALALALLPLIAGLPAVAQMYQGRELVQATLVADTTAVVP
                     GKAFSIGLLLRMAPHWHT"
     gene            complement(322..960)
                     /locus_tag="VFO30_04465"
     CDS             complement(322..960)
                     /locus_tag="VFO30_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418574.1"
                     /translation="MEHPKLLRNGGFNRAYRNIDNPLMNKLRHLASLSVFVLVCSVAS
                     ATTVIPPSFAQLVSQAELIFQGTVTGLKSSWVGQGAERHIETSVTFRVEDAIKGNPGA
                     SYSISLLGGTVDGHTLEVTDTPKFKPGDRDILFVEHNGEQFVPLVGIGFGRFHVQHDA
                     RSGRDFMSTSEGEPLVDVTKLGHAGQIANTATALSPEQFKSAIKAQLGGPGN"
     gene            complement(939..2264)
                     /locus_tag="VFO30_04470"
     CDS             complement(939..2264)
                     /locus_tag="VFO30_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9418575.1"
                     /translation="MTAARGFIGEANAYVLEGPKWGSNPVFQLELGSPGYTLSDGNTS
                     WNTAAAPALSMWNQVIGRIQLGQVVGSTIPVSSGDGYNSMSFATTVFGKSFGSNTLAV
                     TYYTYSSSSMDEADILFNRAQSWNSYRGPLRFNANGAAIAEIQRVALHELGHAIGLNH
                     PDDAGQTVDAIMNSKISDRYTLSSDDIAGAQALYGAPSSQSVPSSNILWQDSITGDRQ
                     IWVMKGTVHTGSVGIGRISTAWNIAAYADFNGDGKRDILWQNSVTGQCTVWLLNGTSV
                     LGGMGFPTVPPPWRIATASDFNGDGKPDVVLQNLVTGQRVIWLMNRTTFVSSVNLGVV
                     PASFTIVGSGDFNGDGKSDILWQNNVTGWRSMWLMNGTTHVSSLLFATVPTSWSIVGT
                     GDFNGDGKRDILWQNQTTGLRVIWLMNRTTPASGVSLGAVPLEWNIRNY"
     gene            complement(2584..3054)
                     /locus_tag="VFO30_04475"
     CDS             complement(2584..3054)
                     /locus_tag="VFO30_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418576.1"
                     /translation="MKATAVILASAAALVVGCRATRDVVETSYHVATAPVRLVHRAIA
                     GGDEPPPPVTSDVVTNPGRPVPPSSPTPSRQWVAESRTSTRQTEAPKTKAPTPSPRTA
                     AAGQQQFPTAKPVPGKPGLVYNPYDPNGAYIDVTGYAPGSKVKDPDSQKIFVVP"
     gene            complement(3116..>4066)
                     /locus_tag="VFO30_04480"
     CDS             complement(3116..>4066)
                     /locus_tag="VFO30_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HET9418577.1"
                     /translation="GGAESYGDDSWTARARELVRELFEIDCAVYFVLNGTAANALALA
                     QICQSHHSVICHRYAHIQTDECGAPEFFTKGAKLLLVGGTNGKIDKQEAEAAIAQQVE
                     LHAQKPRALSITQATELGALYTPDEIHGLAEFAQSRSLLLHMDGARFANAVAAIGCAP
                     KEISWQAGVDVLCFGGTKNGTPAGELVIFFRKELAHEFDYRLKQSGQLVSKMRFLAAP
                     WIGLLTDDVWLRNAQHANKMAAHLATRLAAEGKIDIVFPVEANAVFARMSQQLSTALQ
                     ERGWRFYNFIEPEIYRLMCSWGSTEQKIAEFVADIVALRR"
BASE COUNT          842 a         1194 c         1164 g          866 t
ORIGIN      
        1 atgtgtgcca atgcggcgcc atgcgcagga gcaaaccgat gctgaatgct tttccgggaa
       61 cgacagccgt cgtgtcggca acgagcgtgg cctggacgag ctcgcggcct tgatacatct
      121 gcgcgacagc cggtaatccc gcgatgagcg ggagcaacgc gagagcgaga gcaagtttgc
      181 gagccacaaa gatcactacc accggctaac gcgcgcagca acgcacatca aagccgcgtt
      241 gtgggcagca attggcggtt gctcgccgac gctacagcat cgagcccgct tcccgccgga
      301 cttgcgtcag tcgtgctgca attagttgcc cggcccgccg agctgcgctt tgatcgccga
      361 tttaaattgc tcgggcgaga gcgcggtagc ggtgttggcg atttggccgg cgtggccgag
      421 ctttgtcaca tccacgagtg gttcaccttc actcgtgctc atgaagtcgc gaccgcttct
      481 ggcgtcatgc tgaacgtgga agcggccgaa accgataccg accaacggca cgaattgctc
      541 gccgttgtgt tcgacaaaaa gaatgtcgcg atcgccaggc ttgaacttgg gtgtgtcggt
      601 cacctctagc gtgtggccgt cgaccgtacc gcctagcagc gaaatgctat agctcgcgcc
      661 gggatttcct ttgatcgcgt cctcaactct aaaggtgacg gaagtttcaa tgtgacgctc
      721 ggcaccttgc ccgacccagc tcgatttcag acccgtgact gtgccttgaa agatcaactc
      781 cgcctggcta accagctgtg caaaactcgg tggaatcacc gtcgttgcgc tcgcgacgct
      841 gcagacgagg acgaacacac tgagagaagc gagatggcgg agcttgttca tgagtggatt
      901 gtcgatgttg cggtaagcgc ggttgaagcc gccatttctc agtagtttcg gatgttccat
      961 tccaggggga cagcacccag gctgacaccg ctcgccggag tagtgcggtt cattagccaa
     1021 atgacccgaa gtccagtggt ttgattttgc cataagatat cgcgtttgcc gtcgccgtta
     1081 aaatcgccgg tacctacgat actccagctc gtcggcacag tagcgaatag aaggctgctg
     1141 acatgcgtag tgccgttcat aagccacatc gaccgccaac ccgttacgtt gttctgccag
     1201 agaatatcgg atttcccatc gccgttaaaa tcgccgctgc cgacgatcgt aaagctggca
     1261 ggaacgacgc caaggttcac cgagctgacg aaagtcgttc ggttcataag ccagatcacg
     1321 cgctggccgg tgaccaggtt ctgcaataca acgtccggct tgccgtcgcc gttgaaatca
     1381 gatgcagtgg cgatccgcca cggaggcgga actgttggga agcccatgcc gccaagcacg
     1441 ctggtcccgt tcaacaacca gacagtgcac tgaccagtca ctgaattttg ccagaggata
     1501 tcacgtttgc cgtcgccatt gaaatccgca taggcagcaa tgttccaggc ggttgagatc
     1561 cgaccgatgc ccacgctgcc ggtgtgaact gttcccttca tcacccaaat ctgccggtcg
     1621 cccgtgatgc tgtcctgcca aagaatgttc gacgacggta cggattggga gcttggagcg
     1681 ccgtagagtg cctgtgcgcc agcgatgtca tcgctcgaga gcgtgtagcg gtcgcttatt
     1741 ttcgaattca tgatcgcatc gacggtctgg ccggcgtcgt ctggatgatt caagccgatg
     1801 gcgtggccga gctcatgcaa ggcgacacgc tgaatctcgg cgatggccgc gccgtttgcg
     1861 ttgaatcgca atggcccgcg gtaagaattc catgactgcg cccgattaaa caagatatcg
     1921 gcttcgtcca tcgaggagct cgagtaggtg taatacgtca cggccagcgt gtttgagccg
     1981 aaacttttgc cgaagacggt ggtggcaaac gacatcgagt tgtagccatc gccggagctg
     2041 accgggattg tcgaaccaac aacttgtccg agctgaatgc gcccgattac ctggttccac
     2101 atgcttagcg caggcgcggc ggcggtgttc cacgacgtgt ttccgtccga caacgtgtaa
     2161 ccggggctgc ccagttccaa ctgaaacaca ggattacttc cccacttcgg cccttccagg
     2221 acgtaggcgt tcgcctcgcc aatgaagccg cgagctgcag tcaaaaccac gaaggcaaaa
     2281 actacgccca tgctccgaca ggggattcga tggagtttgg gtcgactcac agacgtagaa
     2341 gcaagcatgg acgcggagta tgcggacggc agcattactc gtgcgcgaca ggcagacatt
     2401 tccgttttcg ccggaaattc tgtcgacgcg caatgaaagc ggaaaagcaa agtgatgatc
     2461 gttccaggag gttagggcag gcggtgcaca aacatacacc tgaaccgcgc aatctgtggt
     2521 ttagttgcgc cctttcgtca aatcaaacgc tgcagtacgc ggagatcccg agcggtgctc
     2581 gtactacggt acgacaaaaa tcttctgtga atctggatct ttaactttgc tgcccggtgc
     2641 gtacccggtc acgtcgatgt atgcgccatt cggatcgtat ggattgtaaa caaggcccgg
     2701 ttttccaggt actggctttg cggtgggaaa ttgctgctgc ccagcagccg cagtccgcgg
     2761 cgagggcgtg ggagctttcg tttttggcgc ctcggtttgg cgcgtgctcg tccgtgattc
     2821 cgccacccac tgccgcgacg gagttgggga acttggcggc actggacgtc cgggattcgt
     2881 caccacgtct gaggtcaccg gcggcggcgg ttcgtcgcca cccgcaatcg ctctgtggac
     2941 gagccgcact ggtgcggtcg cgacatgata tgacgtctca accacgtccc gcgttgcgcg
     3001 gcagccaact acgagcgcag ctgcgctggc taaaattacc gccgtcgcct tcatcccttt
     3061 tcgctctata caaccgtcct cagctagcgc aactgcttga tcatcgaagc cgatctcatc
     3121 gccgcagtgc gacaatgtcc gccacaaact ctgcgatctt ctgctccgtc gacccccacg
     3181 aacacattaa ccgatagatt tccggctcga tgaaattata gaaccgccag ccgcgctcct
     3241 gcaaagcagt ggatagctgc tggctcattc gcgcgaagac cgcatttgcc tcgacgggaa
     3301 acacgatgtc gatcttcccc tcagccgcga gccgcgtggc caggtgcgcc gccattttgt
     3361 tcgcgtgttg tgcgttccgc agccaaacgt cgtccgtcaa caaaccgatc cacggcgcag
     3421 ccagaaacct catttttgac acgagttggc cgctttgttt caaccggtaa tcgaactcgt
     3481 gcgcgagttc tttgcgaaaa aaaatcacaa gctcacctgc cggcgttcca ttctttgttc
     3541 cgccgaagca aagaacatcg accccggctt gccatgagat ttccttcggc gcacacccga
     3601 tcgctgcgac agcgttcgcg aagcgcgcgc cgtccatgtg cagaagcagt gatcggcttt
     3661 gcgcgaactc ggcaagtccg tgaatctcgt caggcgtgta aagggcgccg agttccgtcg
     3721 cctgggtaat gctgagtgcg cggggttttt gcgcatgcaa ttcgacttgc tgcgcgattg
     3781 ccgcctcggc ttcctgcttg tcgatcttac cgtttgttcc gccgaccaga agcagctttg
     3841 cccctttggt aaaaaactcc ggcgcgccac attcgtcggt ttggatgtga gcatagcggt
     3901 ggcagatgac gctgtgatgt gactgacaaa tctgcgccag agcaagcgcg ttggccgccg
     3961 tgccgttgag cacgaagtaa acggcgcaat cgatttcaaa taactcccgc acgagttcac
     4021 gcgcacgcgc cgtccagctg tcgtcgccgt acgattctgc cccgcc
//
LOCUS       DASTDG010000141         9702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_956198, whole genome shotgun sequence.
ACCESSION   DASTDG010000141 DASTDG010000000
VERSION     DASTDG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956198"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VFO30_04485"
     CDS             <1..1829
                     /locus_tag="VFO30_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418578.1"
                     /translation="PEGIKRTDEVEPHESIPRSTSRKLDFAIIAVLLVVVAVFAFNTF
                     RHRKRPELANMPAKSIAVLPFENLSEEKANAYFADGIQDEILTRLSKLADLKVISRTS
                     TRHYQSAPENLSEIAKQLGVAHILEGSVQKSGNAVRVNVQLIKAANDSHLWADTFDRK
                     LTDVFAVESEIAKAIADQLETKMSGSERAAIERPPTSDLEAFELYEHAKALWADVTDP
                     LHAKEKLPEAEELLKAAVTRDPNFMLAWCELAKVHVTLYWLGIDHTAARLDLASAALQ
                     TALRLQSDAGEPHLASGIYQYEAFRNYRKAREELEIARRTLPNNPDVFTYIGFIDRRE
                     GRWIEATRNLERALELDPRNLFILQQLATHTYQPQRRYEDVTRTLERALAIFPGAPLI
                     RINRAEVALDARADIKPYQTTLAALMTENPSVASDVDHPDYSLCERTTEAAVRLLKDY
                     PIGGLSSGYGVNCPHAYWEGVVARWQGDSAKAAAAFSRAREELAPVAERQPEFAAAIS
                     LLGVVDAGLGHKEDALREGRRACELLPVAKDAVSGVALAVNLALIYAWTGEKDLALQQ
                     IAMIERIPNQLSFGMLKLHPYWDSLRGDPRFEQISASLAPQS"
     gene            complement(1801..2739)
                     /locus_tag="VFO30_04490"
     CDS             complement(1801..2739)
                     /locus_tag="VFO30_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HET9418579.1"
                     /translation="MKQGLLLRKGSPEAKDDERIAHAWGFWLPEASLHFWTKNAPYVR
                     DESAAARRIKDALLVAPQPKIFKSVKGGDSFRLPSQNFPNSEFVRVLLARKTHRHFRK
                     QSLSLESVSRLLSLVWGVTGYIHSPRFGKLLRKTSPSGGGRHPGEVYLMALRVKGLRR
                     GLYYYHPSHHRLDRISTPATPTRAWQYCARQQFVKNAAALFLMTAVFPRTIWKYRHPR
                     AYRVVLLETGHLCQTFCLVATWLGLAPFCTAALNDLLIEKDLGIDGIHESVLYVAGVG
                     YPNTAEHPAHSVNRALGHEHHHRRNLAFRIGERVTR"
     gene            3308..5422
                     /locus_tag="VFO30_04495"
     CDS             3308..5422
                     /locus_tag="VFO30_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418580.1"
                     /translation="MPIERRQFFNMSILRVSYRVDARVSNRRGYRLRMTGFFGELKRR
                     KVYRVAIAYVVASWALAQGLAQVLPVFDISNSVIRAVIALLLIGFPVALIFAWIFDIT
                     PSGIERTPEAAPQPMARAQRQRSLILLAALGVLVAAAAGFFLLPRAAAHKIDKSIAIL
                     PFENLSDDKENAYFADGIQDDVLTNLSKIGDLKVISRTSVMGYRGKTGNAREIGKTLG
                     VANILEGSVRRAGNKVRVNVQLIDATNDKHIWAEDYDGDLTDVFKIQTDLAREIAAAL
                     QAKLSPSEKEHLERKPTQNSEAYLAFVRAHDLWSTAVEDLAKLKFSEQTYQHAIDLDP
                     NFALAYARYAQLESWIIHSFENTAERRAKARSLAQRAIELQPQLPEAHLALGFVRYYV
                     DNDFDAAAQEFEIAQRGLPNESEVYLALGAIQRRQGKWDQSNASLEKAVSLNPKDIWT
                     LQNLAINYEMQRNFDAANKTIDRALAIDANAVGPLELKGKIAIEEKGDLSLPEQILAR
                     MDSVPKTPEIEAKLGAIRVTVFLFQRKFESAARAAESLPDAKLTTVPGLLCSKYTAIG
                     VAKKALRDEAGAREVLAKAKALAEADISQNPDSAASHARLAEALAWLGEKDAALAEIE
                     RAQQLLPLSKDAFEGPEMVAKAAEIHAILGDPAGAVSMLDDLLQRPSSVTLGMLKVNP
                     VWDPIRKDPQFQRLIDKYSGKA"
     gene            5409..6170
                     /locus_tag="VFO30_04500"
     CDS             5409..6170
                     /locus_tag="VFO30_04500"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9418581.1"
                     /translation="MAKRNSAAILLVVIATAARAEWVAITSETEPGFLVGLEHRHVVF
                     ENADTNARAIVDIAIFSAKSFVPRLIDNPNRQTDLADAMASTNCVTGVNGGYFDEDFA
                     PVGLLIIDARTIARLQHARLLTGVLLGTTRGMQIVRTREFPAQPATHSAVQAGPFLVD
                     LGKPVHGLEATHEARRTFAASGGRRVALGYCSEISLVELAAILSGGLNDFKIQRALNL
                     DGGSSSAFWFKRRNGSVFSIPEQKPVRDFIGIAPR"
     gene            6174..6941
                     /locus_tag="VFO30_04505"
     CDS             6174..6941
                     /locus_tag="VFO30_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9418582.1"
                     /translation="MMRTFGAFRSAGEFFYGEVVADEVRVLTQPYWLGIESTGEVRKL
                     ADADVDLAVAPSKLIAVGLNYAEHIVEMKRTPLGTPLIWFKAPSSLLPHNGTIEIAFA
                     EHQTDFEIELAVVIGRRAKNISEARASDFIFGYTVGLDISDRDLQKSEKQFGRCKSFD
                     TYTPVGPFVYADVNVADVGIELRQNGQIRQRARTSQMIYSPAQIVAFASEATMLLPGD
                     VILSGTPSGVGPLKAGDELEAKIDNWPPLRNRVVNAR"
     gene            complement(7178..7543)
                     /locus_tag="VFO30_04510"
     CDS             complement(7178..7543)
                     /locus_tag="VFO30_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418583.1"
                     /translation="MRIGHMIGPPLRKLSIRKFPLGTRAPQTPLTAGVSPAAGLNVAV
                     TDAPGAAGAAGDIPGAPAGDIPGAPAGGGIPGAPAGGGIPGWAGDIALPGGGTGAAGF
                     AGAPGEAAGGALGGGAGCV"
     gene            7527..8126
                     /locus_tag="VFO30_04515"
     CDS             7527..8126
                     /locus_tag="VFO30_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9418584.1"
                     /translation="MWPILIVSIVGLTVVIERIFWWAGRWFRRDPKRIEKVFTAIEVG
                     DLAEASRLCRGSRDPVLRMMWNGLNHQHASLQGALQVAAGIEIKRAGRFLVVMDTLVT
                     LAPLLGLLGTVTGLIRSFSFLGNEELAVQAVTGGIAEALIATACGLGIAIFSLVPFNF
                     FTSRVSNLEFELQTAATNLEVMLEANQRARREAEMSTTR"
     gene            8189..8647
                     /locus_tag="VFO30_04520"
     CDS             8189..8647
                     /locus_tag="VFO30_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9418585.1"
                     /translation="MRIGTPIPHKKARIEIIPLIDIMFFLLASFMMVSLTQIHMKGIK
                     VSLPAGQSGETQSKRDYISVSVDANGYSYFDKELMDYDKLRAVLLKVYQENPEAKVFV
                     RGDANTAHVNVIRVLEILRSVGYYKIAFEIKSEAAKGVPAPGAAGQKPGT"
     gene            8650..9255
                     /locus_tag="VFO30_04525"
     CDS             8650..9255
                     /locus_tag="VFO30_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9418586.1"
                     /translation="MAEPVKPLLYRPPPKWHLWSALGGALLIHLGAVLAAMKREEPPV
                     DLGKPPEQVVEATIQEEPQPTPPPEEIIAPPPPAPPPEVKPEYVEERTPPPRPPKPIA
                     KVAPVRQLPMSSAKALAVYAPRPPYPYEARAHKIMGSGVCVVDVDPASGQVTSASMTQ
                     SIGSPILDNATVTAFRQWRFRQGSVSKVRIPITYTLTGSSY"
     gene            9263..>9702
                     /locus_tag="VFO30_04530"
     CDS             9263..>9702
                     /locus_tag="VFO30_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9418587.1"
                     /translation="MKLSSPIPHKRARIEIIPLIDIMFFLLASFMMVSLSQTHMKGIR
                     VNLPAYYAPPSTEPKDYVSLKVRQGNVVLFDDQYVPADDVLPRLYQLHQANPNIKVSI
                     SADGLAMHGEVITLLDEVRRVGITKIGYQIRTAAGPGGGQPGPPP"
BASE COUNT         2139 a         2815 c         2776 g         1972 t
ORIGIN      
        1 caccggaggg gattaagcgg accgatgaag tcgaaccgca cgaatcgatc ccgcgcagca
       61 cgagccgcaa actcgatttc gccatcatcg ccgtcctgct cgtcgtagtc gcggtgtttg
      121 ccttcaatac atttcggcat cggaaaagac cagagctcgc aaatatgcca gcaaaaagca
      181 tcgctgtgtt gccattcgag aatctgagcg aggaaaaggc caatgcctac tttgccgatg
      241 gcattcagga tgagattctg acgcgcttat cgaaactcgc cgacctgaag gttatctcgc
      301 gcacttcgac gcggcattat caaagcgcgc cggaaaacct gtctgaaatc gccaagcaac
      361 tcggtgtcgc tcacattttg gaaggcagcg tgcaaaagag tggcaacgcc gtgcgcgtga
      421 acgtgcagtt gatcaaagca gcaaatgatt cgcatctctg ggccgatact ttcgatcgca
      481 aactgactga cgtctttgcg gtcgaaagtg aaatcgcgaa agcgatcgcc gaccagctcg
      541 agacgaagat gtctgggagc gagcgcgcgg cgatcgagcg gccaccaacc tctgacttgg
      601 aggcgttcga actctacgag cacgccaaag ctctttgggc tgacgttacc gatccgctgc
      661 acgcaaagga gaagctgcca gaagcggagg agctcctgaa agcagccgtc acccgcgacc
      721 cgaatttcat gctcgcctgg tgtgagctcg caaaagtgca cgtcacgttg tactggctcg
      781 gaatcgatca caccgcagcg cgtcttgatt tggctagtgc ggcgctacaa acggcgttgc
      841 gtcttcaatc ggatgcgggc gagccgcatc tcgcttccgg gatctaccag tacgaagcgt
      901 ttcggaatta tcggaaagcg cgcgaggaat tggagatcgc ccgccgcaca ctaccgaaca
      961 acccggacgt ttttacctac atcggcttta ttgatcggcg cgaaggcaga tggatagagg
     1021 cgacacgcaa cttagaacgc gcgttggaat tggatccgcg caacttgttt atccttcagc
     1081 aactcgccac tcacacctat cagcctcaac gccggtacga agatgtcacc cgaacattgg
     1141 agcgtgcatt ggcgattttc cccggcgctc cattgatccg aatcaatcgg gcggaagtgg
     1201 cgttggacgc gcgtgcggat atcaaaccgt atcaaaccac attggccgcg ctcatgaccg
     1261 agaatcccag cgtcgcgtcg gacgtggatc acccggatta ctcgctatgc gaacgaacga
     1321 cggaagcagc cgttcgcctg ctgaaagatt acccgatcgg cggcctctcg agcggttacg
     1381 gcgtcaactg tccgcatgct tattgggaag gtgttgttgc tcgctggcag ggcgattcgg
     1441 cgaaagccgc ggccgcgttt agccgtgcac gagaagaact ggcgccagtc gcggagaggc
     1501 agccagagtt tgcggcggcc atcagtttgt tgggcgtagt cgatgccgga ctgggccaca
     1561 aagaggacgc gctgcgcgag ggcaggcgag cttgtgagtt gctgcccgtc gccaaggatg
     1621 cagtgagtgg tgtcgcgctg gccgttaacc ttgcactaat ctacgcctgg actggcgaga
     1681 aagacctagc gctccaacag attgcgatga tagagcgcat tccaaatcag ctgagctttg
     1741 gtatgctcaa actgcatccc tattgggact ccttgcgcgg tgacccgcgg ttcgaacaga
     1801 ttagcgcgtc actcgctccc caatcctgaa cgccaggttc cttctgtgat gatgttcgtg
     1861 gccaagagca cgattgacgc tgtgtgctgg atgctctgct gtatttggat agcccacgcc
     1921 ggcgacgtaa agcaccgact cgtggatacc gtcaattcca aggtcctttt caatcaatag
     1981 atcgttgagt gctgccgtgc aaaacggcgc caagccaagc catgtcgcca ccaagcaaaa
     2041 cgtctggcac aaatgacccg tctccaaaag caccacgcga tacgcgcgtg ggtgacgata
     2101 cttccagatc gtccgaggga aaacggctgt cattaaaaac agcgccgcgg cgttttttac
     2161 aaactgttgc cgggcgcaat attgccatgc tctagtgggc gtggccggcg tgctaattct
     2221 gtcgagccga tgatgcgatg ggtgataata atacagcccc cgtcgcagac ctttcacgcg
     2281 taatgccatt agatacactt cgcccggatg ccgcccgcca cccgaggggc tcgttttgcg
     2341 caacaacttc ccgaatcgag gcgaatggat gtagcccgtc acaccccaaa ccagcgagag
     2401 aaggcgtgat acactctcga gactcagact ttgcttccgg aaatggcggt gggtttttcg
     2461 cgccagcagg acgcgcacga actcagaatt aggaaaattt tgcgacggga gccgaaagct
     2521 gtctccacct ttgaccgact tgaaaatctt tggctgaggc gccacgagta acgcgtcttt
     2581 gatgcgacga gccgcagccg actcgtctcg aacgtacggc gcgttttttg tccaaaagtg
     2641 caaacttgct tcaggcagcc aaaagcccca cgcgtgggcg atccgctcat cgtccttcgc
     2701 ttctggtgaa cctttccgta gcagcaggcc ctgcttcaac aactgcgaaa ctgcagaccg
     2761 cacacttctc ggcgtgaaat tagagaaatg gtggacggct tgccgcgaag ttcgccacgt
     2821 gccgaaaaaa tcgagaactt cgcaggcaag cggtcctgct gcgattgtca gccgactagc
     2881 gaaattgcta aaacaaagcc gtccgtcttt ccagtaagcc aacaagttgc tggcgcgtcg
     2941 gaactgaatc cgctctcgca tttgtcacgg cgtgatgccg tttactggca gggctgtggt
     3001 tgttttgcgc gagtgcaaca gtctgctctg agatactcta gcaaattggc ccggctatgc
     3061 cttatgcggt atcatcatca accgggcttt cactttgtcc aaatcgaggg acattctttg
     3121 atgggctctc ttgagttttt gcacatccgc aatcgccttt gtaacatgcg gatggcgctt
     3181 tctcgcaggt tttcgctttg gcatggttgc tcctttctga attcgaacgg cgagcgtaat
     3241 cagatcggca gaggcgtgtc aataacttga gcagtgggtc gttaggttcg gggcgaatcg
     3301 tcgccgcatg ccgatcgaga gacgccagtt tttcaacatg tcgatcttgc gggtgtctta
     3361 tagagtggat gctcgcgttt ccaaccgccg cggctaccgt ctccgcatga ccggcttctt
     3421 cggagaactc aagcgccgaa aagtctaccg cgtggcgatc gcgtatgtgg tggcgagttg
     3481 ggctctcgcg caaggtctcg cccaagtcct tccggttttt gacatttcga actcggtcat
     3541 ccgcgcagtt atcgcgttgc tgctgatcgg tttcccggtg gcgctgattt tcgcctggat
     3601 attcgacatt accccgagcg ggatcgaacg tacgccggaa gctgcgcccc agccgatggc
     3661 gcgagcgcaa cggcagcgca gtttaatatt actcgccgcc ttgggggtcc tcgtcgcggc
     3721 agctgccggg tttttcctgc tcccgcgcgc cgctgctcac aaaatcgaca aatcgatcgc
     3781 catcctcccg ttcgagaacc tgagcgacga caaagagaac gcttatttcg ccgatgggat
     3841 ccaggatgac gtcctcacca atttgtccaa aatcggagac ctgaaagtga tttcccgcac
     3901 atcggtaatg ggttaccgcg gaaaaaccgg aaatgcacgc gagatcggga agacgttggg
     3961 cgtggcaaat attctcgaag gcagcgttcg ccgcgccggc aacaaagtgc gggtgaacgt
     4021 gcagttgatc gatgcgacga acgataaaca catctgggcg gaagattacg acggcgattt
     4081 gaccgacgtt ttcaagatcc aaaccgatct cgcccgtgaa atcgccgccg ctctgcaggc
     4141 gaaactttca ccgagcgaga aagaacacct cgagcgcaaa ccgactcaaa acagcgaggc
     4201 ttatcttgcc tttgtccgag cgcacgatct ctggagcacc gccgtcgaag acctggccaa
     4261 attgaaattc agcgagcaga cataccagca cgcgatcgac ctcgatccca atttcgcgct
     4321 cgcgtacgcc cgttacgcgc agctggagag ctggattatt cacagcttcg agaacaccgc
     4381 cgaacgccgg gccaaagcgc gctcgctcgc gcaacgcgcg attgaattgc agccgcagct
     4441 gccggaggcg catcttgctt tggggttcgt tcgctattac gtcgacaacg atttcgacgc
     4501 cgccgcacag gaatttgaga tcgcgcagcg cggtttgcct aacgaatcgg aagtttacct
     4561 cgccctcggc gcgattcagc ggcgccaggg caaatgggac caatccaacg ccagtttgga
     4621 aaaagcggtc agcctcaatc cgaaagacat ttggacgctg cagaatctgg caatcaatta
     4681 cgaaatgcag cgcaatttcg acgcggcgaa caaaacgatc gaccgcgcgc tcgcgattga
     4741 cgcgaatgca gtggggccgc tcgagctcaa aggcaagatc gccatcgaag aaaagggcga
     4801 cctcagcctt cccgaacaga ttctggcgcg aatggattcg gttccaaaaa caccggaaat
     4861 cgaagcgaaa ttgggcgcga tccgcgtcac ggtgtttttg tttcagcgga aatttgagtc
     4921 cgcggcacgc gctgccgaga gcctgccgga cgcgaagctg acgactgttc ccgggttgtt
     4981 gtgtagcaaa tacaccgcca tcggcgttgc caaaaaagca ctgcgtgatg aggccggcgc
     5041 gcgtgaagtt ctcgccaaag cgaaagcatt agccgaagcc gacatcagcc aaaacccgga
     5101 cagcgcggcc agtcatgcgc gtttagccga agcgctcgcc tggctgggcg aaaaagacgc
     5161 ggcactggcc gaaatcgagc gcgcgcagca attacttccg ctgagcaaag acgcgtttga
     5221 gggaccggag atggtggcga aagcggcgga gatccacgcc attctcggtg acccggcggg
     5281 cgcagtgtca atgctcgacg atctgttgca gcggccaagc tcggtcacac tggggatgct
     5341 gaaagtcaac ccggtctggg atccgatccg aaaggacccg cagtttcagc gcttgatcga
     5401 caaatatagt ggcaaagcgt aattccgcgg cgattctgct cgtcgtcatt gcgacggccg
     5461 cgcgcgcgga gtgggtcgca attacttccg aaacggaacc aggcttcctc gtcggactgg
     5521 aacatcgaca tgtggttttt gagaacgcgg acacgaatgc acgcgcgatt gtcgatattg
     5581 cgattttctc ggccaagtcg tttgtcccgc gattgattga taatccaaac aggcaaaccg
     5641 atctcgccga tgcgatggcg agtacaaact gtgtcaccgg agtgaacggc ggctatttcg
     5701 acgaggattt cgcgccagtg ggccttttga ttatcgatgc caggacaatt gctcgattgc
     5761 aacacgcgcg cttgttgacg ggcgtgttgc tcggcacaac ccgaggcatg caaatcgtgc
     5821 gaacccgcga gtttcccgct cagccagcca ctcactcggc ggtacaggcc ggaccgttcc
     5881 ttgtcgacct tggaaaaccg gtgcacggcc tcgaggcgac tcacgaagca cgccggacct
     5941 ttgcggccag tggtggcagg cgtgtcgcgc ttggctactg ctcagagatt tcgctcgtcg
     6001 agttagctgc gattctttct ggtggtctga acgatttcaa gattcagcgc gccctcaatt
     6061 tagatggcgg ctcctccagc gcgttctggt tcaaacgccg caacggcagc gtgttttcca
     6121 ttccggaaca aaaaccagtg cgcgatttca tcggcatcgc gccacgttaa tggatgatgc
     6181 gtacctttgg cgcctttaga tccgccggtg aattcttcta cggcgaagtc gttgcggatg
     6241 aagtgcgcgt gctgacgcag ccgtactggc tcggcatcga atcgaccggc gaagtgcgga
     6301 agctggccga tgcagatgtc gatcttgcag tggccccgtc gaagctgatc gcagtcggtc
     6361 tcaattatgc cgagcacatt gtggagatga agcgcacccc gctgggcacg ccgctcatct
     6421 ggtttaaggc accgagctca ttattgccgc acaatggcac gatcgagatc gcgttcgcgg
     6481 aacaccaaac cgatttcgaa attgaactcg ccgtcgtgat tggccggagg gcgaaaaaca
     6541 tttcggaagc ccgcgcgtcc gattttattt ttggctacac ggtcggcctc gacatcagcg
     6601 atcgcgattt gcagaagagc gaaaaacaat tcggtcgctg caaatctttc gacacctaca
     6661 caccggtggg gccgttcgtc tacgccgatg tcaatgttgc cgatgtcggc atcgagctgc
     6721 gccagaacgg ccaaatcaga caacgcgcgc gcacgagtca gatgatttat tcaccggcgc
     6781 agatcgtcgc gtttgcgagc gaggcgacga tgttgctgcc cggcgacgtc attttgagcg
     6841 gaacgccatc gggcgtcggc ccgctcaagg ccggcgatga actggaagca aagatcgaca
     6901 attggccgcc gttgcggaac cgcgttgtca acgcgcgata gctcttcgcg caccgcttac
     6961 atcccaacgg cgcagttgtt tcgaaataaa agatagcaac agcttcggct caccaacagt
     7021 taaatcgaca aacgtgaaaa gttttgctcg actcggccta aactcgtttg cgataaagac
     7081 atacctcaaa attgaccagc cgttaaatgc aatcgtctga acctatgaaa acaatcatca
     7141 ctctctgcct cagtctcgtc gcgctgactg cgttcgctca aacgcaaccc gcgccaccgc
     7201 ccaacgcgcc gcctgccgcc tcaccgggcg cccctgcaaa tccagccgcg ccggttccgc
     7261 cgccgggaag cgcgatgtcg ccggcccaac cgggaatgcc gccgccggcc ggtgcaccgg
     7321 gaatgccgcc tcccgccggt gcgccaggaa tgtctccggc cggcgcgcca ggtatgtctc
     7381 cggcggcgcc agctgcgccg ggcgcatcag ttaccgcgac attgagtcca gccgctggtg
     7441 aaactccagc cgtcagcgga gtttggggag cacgagtgcc gagcgggaat ttcctgatcg
     7501 acaatttccg caagggcggc ccgatcatgt ggccgattct catcgtgtcg atcgttggct
     7561 taactgtcgt gatcgagcgt atcttttggt gggcgggtcg atggttccgg agggacccga
     7621 agcgcatcga aaaagttttc accgcaattg aagtcggtga cctggccgaa gcatcgcggc
     7681 tgtgccgcgg ctcgcgtgac ccggtgctac gcatgatgtg gaacggcctg aatcaccagc
     7741 acgcatcgtt gcagggcgca ctgcaagtgg ccgccggcat tgagatcaag cgcgccggcc
     7801 gcttccttgt tgtgatggac acgctggtca cactggcgcc gctgctcggt ttgttgggaa
     7861 ccgtcaccgg actcattcgg tcgttcagct tcctcggcaa cgaggagctg gccgtgcaag
     7921 cagtgaccgg cggtatcgcc gaggcgctga tcgctacggc gtgcggtctc ggcatcgcca
     7981 tcttttcact cgtgccgttt aactttttca cctcgcgcgt gtctaactta gagttcgagc
     8041 tgcaaaccgc agccacgaac ctggaggtga tgctggaagc gaaccaacga gcgcggcgcg
     8101 aagcggaaat gtccaccacc aggtaaaatg ctcgagctgg aaagaacgtc atgaacgccg
     8161 aggcaatggc aatagcaaac gcaaacgcat gagaatcggc actcccattc ctcacaagaa
     8221 agcgcggatc gagatcattc cgttgatcga catcatgttc tttcttttgg ccagtttcat
     8281 gatggtgagc ttgacccaga tacacatgaa aggcatcaag gtgagtttgc ccgccggcca
     8341 atcgggcgaa acgcagtcga aacgcgatta catcagcgta tcggtcgatg cgaatggcta
     8401 ttcttacttc gacaaggagc tgatggatta tgacaagctc agggcggtgc tgctgaaggt
     8461 gtatcaggaa aatcccgagg cgaaggtctt cgtgcgcggt gacgcaaaca cggcccacgt
     8521 caacgtgatc cgcgtgctcg agattctcag atcggtcggc tactacaaaa tcgcttttga
     8581 aatcaagagc gaggctgcca aaggcgttcc cgctccggga gcggccggtc aaaaacccgg
     8641 gacttgatta tggccgaacc cgtcaaaccg ctgctttatc gcccaccgcc caagtggcat
     8701 ctctggtcag ctcttggcgg tgcgctgctc atccacctcg gtgccgtgtt ggcggccatg
     8761 aagcgggaag agccgccagt ggatctcggt aagcctcccg aacaagtagt ggaggcgact
     8821 attcaggagg aaccacaacc cacaccaccg cccgaggaga ttatcgctcc gcctccgccc
     8881 gccccgccgc cggaggtcaa gccggagtac gtcgaggaac ggacgccacc gccgcggccg
     8941 cccaagccga tcgcgaaagt ggctcccgtc agacaactgc cgatgtccag cgccaaggcg
     9001 ctcgccgtct atgcgccgcg gccgccgtat ccgtatgaag cccgtgcgca caagatcatg
     9061 ggcagcggag tttgtgtcgt cgacgtggat ccagccagcg gtcaggtgac cagcgccagc
     9121 atgacccaaa gtattggcag ccccatcctc gacaacgcca ctgtgactgc attcaggcaa
     9181 tggcgatttc gacaaggctc ggtgtccaag gtaagaatcc ccattaccta tacattgacc
     9241 ggctcctcct attagttacg caatgaaact ttcctcgccc attccacaca aacgagcccg
     9301 gatcgagatc atcccgttga tcgacatcat gttcttcctc ctcgccagtt tcatgatggt
     9361 gagcttgagc cagacgcaca tgaaaggcat ccgggtcaat ctgccggcct actatgcgcc
     9421 gccgtcgacc gagccgaaag attacgtctc cctcaaggtg cgccagggca atgtggtttt
     9481 gtttgatgac cagtatgtac cggctgatga tgttttgccg cgcctttacc aactgcacca
     9541 ggccaatcca aacatcaaag tctccatcag cgccgacggg ttggccatgc acggtgaggt
     9601 gattacgctg ctggatgaag tgcggcgggt cggaatcacc aagatcggat atcagatccg
     9661 aacggcagct gggcccggcg gcgggcagcc cggtccgcct cc
//
LOCUS       DASTDG010000142         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_229532, whole genome shotgun sequence.
ACCESSION   DASTDG010000142 DASTDG010000000
VERSION     DASTDG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229532"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1736
                     /locus_tag="VFO30_04535"
     CDS             246..1736
                     /locus_tag="VFO30_04535"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9418588.1"
                     /translation="MRKEILILFGAVALSTSHAIAQAAACDHEHEQQGVKYTCVMHPE
                     VVMDHPGTCPKCGMKLVPVRQSKRPMPNSERAARSHEMPMEQMSMQSSINLADPMNRE
                     SSGTAWVPDSTPMYGRMFMLGDDMLMLHGAIFPRYTNVSTRRGDDRIDAPNWIMAMYS
                     HPFSETTQLGGRLMMSLDPLTEGGRGYPLLLQSGESWHDQPLHDRQHPHDLFDELSLS
                     LSHKVDVDLSGYFYFGYPGEPALGPPTFMHRLSAMDNPDAPLGHHWQDSTHVTFGVAT
                     AGIQWRNVKVEGSSFTGREPDEDRYDFDQPRFDSFSGRVSWNPTQNLALQFSHGYIRS
                     PEALEPDVNRHRTTASAIYNLPLGHDTNWSNSFVWGRNNDAGEKTNSVLLETNYQRHR
                     DTIYARWEWVEKSGHELVLKDVDLAKVFPINAVTVGYVRDLSHGNGIDVGLGGQFTLD
                     FWPNDLDRYYGGGPSYGFEVFLRIRPSLHSHAERVHSEDIAGMQEM"
     gene            1842..2909
                     /gene="glpX"
                     /locus_tag="VFO30_04540"
     CDS             1842..2909
                     /gene="glpX"
                     /locus_tag="VFO30_04540"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET9418589.1"
                     /translation="MTHRLPKQDYHDAERIIEFDFVRATEAAALNSLRWLGRGDKDAA
                     DAAACDAMRGMFDLMNICGEVVIGEGIKDEAPGIFKGEQLGTWYPGSPPFDIAVDPID
                     GTTNISKGAPNSISCIAAASPEEGVKVALRDILSFYMSKLAYGPRVVEYMKKRGDTLQ
                     IDMPIAEILAIVARALDKRVQDVAVMMLDRPRHKEIVEQIRASGASLRMIGDGDIAAA
                     MAPSLPESDIDLYMGIGGSPEAVLAAAGIKCLGGEMQCKMWPRDDKERQKLIGEGCEQ
                     ELERVFTADDLAVGENIIFCATGISDSALLRGVKSQGTTAITSSILMRAKSKTVRFIR
                     ARHNLQNKTIHLRSTNREHLI"
     gene            3078..3482
                     /locus_tag="VFO30_04545"
     CDS             3078..3482
                     /locus_tag="VFO30_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HET9418590.1"
                     /translation="MRRRQVPKTELSKENGCVVDTKWRLGSLSYHASAIGKEEIKPSS
                     EIVRLPELVIYDHICFGGAYARTNLSFHYIGDFWDDRISSVIVVSGIWRFYRDEYYKG
                     PHWDLGPGYYESFFTEKGPDDVVSSFQCIALT"
     gene            complement(3578..4567)
                     /locus_tag="VFO30_04550"
     CDS             complement(3578..4567)
                     /locus_tag="VFO30_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9418591.1"
                     /translation="MAKFVAIIGAGVSGLTCGVLFAERGFRTTIFAKQIGLQTTSGAA
                     AALWFPYDCEPRHKVIPWALETYRVLVGLADVPRSGVSMIELRQYSRTGNLQIPHWAI
                     PLGASFIPSSPFVMSSGVETSLTVVPAAFKAGFAMHVALMDTTIYLSHLATRFVAAGG
                     SINGGVRFETLEDVDHKFDLVINCAGIGARELVQDVDLEPHRGQVAIVPKIDNLSCAI
                     VCDDEPLMYAIPRVQDCVFGGTNELSDNLAVDPKSTARIVAECAGVLNIEKPRVVAER
                     VGLRPYRKSGVRLERDKLRDGRTIIHNYGHGGSGFTLSWGCAEEVFKIADPSG"
BASE COUNT         1059 a         1359 c         1417 g          912 t
ORIGIN      
        1 gatgtcgatc ttcccgtcga tgcgacggcg gtctcgagag ctgcgcatgt cactttcgtg
       61 cccgagacag tcggcaccgg tccaccgggc acgacttcat tcgttgaatt gatcgggagc
      121 gttcaagcac acgctccgcc ttcacgcgcc taactggcgc cttgaaacgt agggcaaccg
      181 ctccggttgc catcctttgc tggcagggcg aagcgcctgc cgtcctacaa aaccgctgca
      241 aaaaaatgag aaaagagatt ctgattctgt tcggtgcggt cgcgctttca acgagccacg
      301 cgatcgcgca agcggccgcc tgcgatcacg agcacgagca acagggagtg aaatacacct
      361 gcgtcatgca tcccgaagtc gtgatggacc atccgggcac ctgcccgaaa tgcgggatga
      421 agctcgtccc ggtcagacaa agcaaacgcc caatgcccaa ttcagaacgc gcagcgcgct
      481 cgcacgaaat gccgatggag cagatgtcga tgcaatcctc gatcaacctt gccgacccga
      541 tgaatcggga gagctccggc acggcgtggg tcccggattc aacgcccatg tacggccgca
      601 tgttcatgct cggcgacgac atgctcatgc tgcacggagc gatttttccg cgttacacca
      661 acgtgagcac gcgacgtggc gacgatcgca tcgatgcacc gaactggatc atggccatgt
      721 attcgcatcc attcagcgag acgacccaac tcggggggcg attgatgatg agtctcgatc
      781 cgctgaccga aggcgggcgg ggttacccgt tgctcttgca aagtggtgaa tcctggcacg
      841 atcaaccgct gcacgaccgg cagcatccgc atgacttgtt cgacgaactc tccctcagtc
      901 tttcacacaa ggtcgatgtc gatcttagcg gctattttta tttcggttat ccgggcgaac
      961 cggcactcgg gccaccaacg ttcatgcatc ggttgtcagc gatggataat cccgacgccc
     1021 cgctgggtca tcattggcag gactcgaccc atgtgacttt tggcgtcgcg accgcgggga
     1081 tccagtggcg gaacgtaaaa gtcgagggca gctcattcac gggtcgcgag ccggatgaag
     1141 atcgttacga cttcgatcag ccgcgattcg attcattcag cggccgcgtt tcctggaatc
     1201 cgacgcaaaa cctggccctg caattttcgc acggttacat ccgaagccca gaagcactcg
     1261 agccggatgt gaaccgccat cgcacgacgg cgtccgcgat ttacaatcta ccgctcggcc
     1321 acgacactaa ttggtcgaac agttttgtct ggggcagaaa caacgatgcg ggcgagaaga
     1381 cgaattcggt tctccttgag acaaattatc agcgccatcg cgacaccatt tacgcgcgct
     1441 gggaatgggt ggagaaatcc ggacacgagc tcgtgctgaa agatgtcgat cttgcaaaag
     1501 tatttccgat caacgcggtg acagtcggtt acgtgcggga tctctcccac ggcaatggga
     1561 tcgatgtcgg tctgggcggg cagttcacac tcgatttttg gcctaacgac ctcgaccgct
     1621 attacggcgg cggtccgagt tacggcttcg aggttttcct gcgcattcgg ccgtcgctcc
     1681 actctcatgc cgaacgcgta cattccgaag atatcgccgg aatgcaggaa atgtagtggc
     1741 aggcgtgccg cctgcagaca acagaaatgc agccgacacg gctgcctcta caacatcagc
     1801 gttcttgtca caacgactta gcgcgtgaat cttatcggcc gatgacgcac cggctgccga
     1861 agcaggatta ccacgacgcc gagcgcatca tcgaattcga ttttgtgcgc gcgacggaag
     1921 cggcggcgct gaattcgttg cgctggctcg gccgcgggga caaggacgcg gccgacgccg
     1981 cggcctgcga cgcgatgcgc gggatgttcg atctgatgaa catctgcggc gaggtcgtca
     2041 tcggcgaagg catcaaggac gaagcgccgg gtattttcaa gggcgaacaa ctcgggacct
     2101 ggtatcccgg ttcgccgccg ttcgatatcg cagtcgatcc gatcgacggc acgacgaaca
     2161 tttccaaagg cgcgccaaat tcgattagtt gcatcgcggc ggcgagcccg gaggaaggag
     2221 tgaaggtggc gttgcgcgac attctgtctt tttacatgtc gaagctggct tacggtccgc
     2281 gcgtcgtcga gtacatgaaa aaacggggcg acacgctcca aatcgatatg ccgatcgcgg
     2341 agattctggc gatcgtcgcg cgcgccctgg ataaacgggt gcaggacgtc gcggtcatga
     2401 tgctcgaccg cccgcggcac aaggaaatcg tcgaacagat ccgtgcaagc ggtgcgtcgt
     2461 tgcgcatgat cggcgacggc gacattgccg cggccatggc gccatcgttg ccggaaagcg
     2521 acatcgatct ctacatgggc atcggcggtt cgccggaagc ggtgctcgcc gcggcaggca
     2581 tcaaatgcct cggcggcgaa atgcaatgca aaatgtggcc gcgcgacgat aaagagcggc
     2641 agaagttaat cggcgaaggt tgcgaacaag aactcgagcg cgtgtttacg gccgacgatc
     2701 tcgcagttgg cgaaaacatc atcttctgcg caaccggcat cagcgacagt gcgctcttgc
     2761 gcggcgtgaa atcccaaggc acgaccgcca tcaccagttc gattctgatg cgggcgaaaa
     2821 gcaaaacggt ccggttcatt cgcgcccggc acaatttgca gaacaaaacc atccatctgc
     2881 gttcgaccaa tcgggagcat ttgatctagg aacatagacc tgcggtctgt gcggccagcg
     2941 gagttgtact ccgttgactg ccgccgaaag aaactcgcag gcgaggacgc ccgcgagccg
     3001 cacaggctag atgcctatgt tccggctcga cagcgccgtt tggcgtgtac aagatcgaca
     3061 tcgaaccttt gcggcttatg agaagacgac aagtacccaa aactgaactt tccaaggaga
     3121 acggctgcgt cgtggatacg aaatggagac tgggctcgtt gagttatcac gcgtcggcca
     3181 tcggtaaaga agagatcaaa ccatcgagcg agatcgtgcg tctgcccgag ctcgtcattt
     3241 acgatcacat ctgtttcggc ggcgcgtatg cccgcacaaa cctgagcttc cactacatcg
     3301 gcgatttctg ggacgaccgc atcagctcgg tgattgtcgt cagcggcatc tggcgctttt
     3361 atcgcgacga atattacaag ggcccgcact gggacctcgg ccccggctac tacgaaagct
     3421 tctttaccga aaaaggtccc gacgacgtcg tcagctcctt tcagtgcatc gcgctgacat
     3481 aaaatcgatt cggtttaaca aattgcgtcc gcacgatcac gatatgtctg ggtccgcaat
     3541 ggcgaggacg ccatcgtcag cacgcgagac gcgtgcacta cccagaagga tccgcgatct
     3601 taaagacttc ttcagcgcag ccccaggaaa gggtgaagcc agagccgccg tggccatagt
     3661 tgtggatgat ggtgcggccg tcgcgcagtt tatcgcgttc aaggcggacg cctgatttgc
     3721 gatacgggcg cagaccgacg cgctcggcga cgacgcgcgg cttctcgatg ttcaacacgc
     3781 ccgcgcactc ggcgacgatg cgcgcggtcg attttggatc gacggcgaga ttatcgctga
     3841 gctcgttggt gccgccgaag acgcaatcct gcacgcgggg aatggcgtac atgagcggtt
     3901 cgtcgtcgca cacgatcgcg caggagagat tgtcgatctt cggtacgatg gcgacttggc
     3961 cgcgatgcgg ctcaagatcg acatcttgga cgagttcgcg cgcaccgatc ccggcacagt
     4021 taatgaccag atcgaacttg tgatcgacgt cttcgagcgt ttcgaaacgc acgccgccgt
     4081 tgatggaacc gcctgcggcg acgaatcggg tcgcgagatg gctgagataa atcgttgtat
     4141 ccatcagcgc gacatgcata gcgaatcccg ctttgaaggc cgccggaaca acagtaagag
     4201 atgtctcgac tccgctcgac atgacaaaag gggaactcgg gatgaaagaa gcgccgaggg
     4261 gaattgccca atggggaatt tgcaggttgc cggttcgcga atattggcgc agctcgatca
     4321 tcgagacgcc gctgcgcgga acgtctgcaa gaccgacaag tactcgatag gtttcgagcg
     4381 cccaagggat gaccttgtgg cgcggctcgc agtcgtacgg aaaccaaagc gcggcggccg
     4441 ctccggatgt ggtttgtagc ccaatttgct tggcaaagat agtggtgcga aatccgcgct
     4501 cggcaaaaag cacgccgcag gtcaatccgg aaacgccggc accgatgatt gccacgaatt
     4561 ttgccacgcg acaacgttgt agggcaggcg ctccgcctgc caactaaaaa gcggcgagcg
     4621 gtgcgcttgc cctacaaatt cgcgtgtgaa catgcgtgcg aaaagagcca cgatcattta
     4681 ggctcggacc gagatggttg cgattaaccg agaggcgctg acggaaaaag tcctcgcggg
     4741 cgaacgg
//
LOCUS       DASTDG010000143         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1191163, whole genome shotgun sequence.
ACCESSION   DASTDG010000143 DASTDG010000000
VERSION     DASTDG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191163"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..538)
                     /gene="nrdR"
                     /locus_tag="VFO30_04555"
     CDS             complement(26..538)
                     /gene="nrdR"
                     /locus_tag="VFO30_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9418592.1"
                     /translation="MRCPKCGSRDDKVVDSRQSRDASSIRRRRECLSCGYRFTTYEEI
                     ERSDLRVIKRDRTHEAFDRRKLAGSIAKAFEKRSTSLLALEQLVDAIEHELQTGGREV
                     QSTDIGAKVLEKLRGIDEVAYLRYASVHRQFKEVDQFVDAIQSLGRRVKPDRAQPELF
                     PPDQLNHGRI"
     gene            complement(605..3076)
                     /locus_tag="VFO30_04560"
     CDS             complement(605..3076)
                     /locus_tag="VFO30_04560"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HET9418593.1"
                     /translation="MAKALLIAEKPSVASDLARALGGFKKEGDHYESERYLISSALGH
                     LVELALPTELDVKRGKWSFANLPIIPEEFQLKPIEKTKARFNMLKRLIQRKDVDLLIN
                     ACDAGREGELIFRYLMKLSGVKKPVRRLWLQSMTLDSIREAFQHLRSDEEMIPLANAA
                     VCRSESDWLVGINGTRAMTAFNNRTGGFQLTPVGRVQTPTLAILAEREDKIHDFKPRT
                     YFEVFGDFEVNAGAYRGRWFREDFTKDGDEDARAERIWDKATAEEIKQRCLGKTGVAT
                     EERKATTQAPPLLYDLTSLQRDANARGFSAKRTLQIAQQLYERHKVITYPRTDSRFLP
                     EDYLPTVKATLSKIDNPHARTALGRHWVKLSKRIFNDAKISDHFAIIPTGTTPHGLDE
                     VQQKIYDMIVRRFISVFFPAAQFEVTTRITRVEKDAFKTEGKIIKDPGWLAVYGREAV
                     SGESGEESLVAITPNEQAKVRTIDVTQNETKPPARFTEATLLSAMEGAGKLVEDEELR
                     EAMRQKGLGTPATRSAIIEGLIHDGYVTRQGRELITTAKGLALITLLRGIGVTELCSP
                     EMTGEWEYKLKRMEKGAMKRKEFMAHIKEFTREIVEKARNFEGDNVTGNFEPLEVKCP
                     KCGGGPFDEEYRTFKCRSCGLLIWKTMAGRLFERNEIAKLLTEGRVGPLEGFRSKMGR
                     KFTAEVKLGEDFKQEFDFGEGAHDQPIKIDIHKHERLGLCPVCQKGQVYVLERAYACE
                     NAVTSPKTCSFRVSKSILHRDIPKEQVQKLMTTGKTDLLPRFVSKKGRPFSAHLKLEN
                     GKVGFEFAAKPPKAKKPTARKAVAA"
     gene            complement(3161..>3865)
                     /gene="dprA"
                     /locus_tag="VFO30_04565"
     CDS             complement(3161..>3865)
                     /gene="dprA"
                     /locus_tag="VFO30_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9418594.1"
                     /translation="KLAYQLAYAGLTIISGLARGIDTAAHQGALAAKGRTIAVIGSGL
                     AKLYPPENQALAEKIHAGNGAIVSEFSMEIEPDRHTFPMRNRIISGWSHGILVVEAGL
                     NSGALITAAQALEQGRSVYAVPGHINAPSAMGSNRLIQQGAKLVMDASDILEDLQILL
                     PEAKPLPEAAVRLLPPLSDEERRVYEAIDAAETPIDQISAKCKLPSGAVSSTLLSLEL
                     KRLVKQLPGKYFVKLG"
BASE COUNT          723 a         1150 c         1067 g          925 t
ORIGIN      
        1 cgcacgcagg tagggaaatt cttctttaaa tgcgaccatg gttcagttga tccggaggaa
       61 acagttcggg ttgcgctcga tctggtttca cgcggcggcc gagcgactgg atggcatcga
      121 caaattgatc gacctccttg aactgccggt gcaccgacgc gtaacgcagg tacgccactt
      181 catcgatgcc gcgcaatttc tcgagcacct tcgcgccgat gtccgtcgat tgaacctcgc
      241 gtccaccggt ttgtaactcg tgctcgattg catcgacgag ctgctcgagc gcaagcaagc
      301 ttgtcgatct tttctcgaac gccttcgcga tgctgccggc gagcttgcgc cggtcgaacg
      361 cctcgtgcgt tcggtcacgc ttgatcaccc gcaggtccga acgctcgatc tcctcgtacg
      421 tcgtaaatcg ataaccgcag cttagacatt cgcgccggcg gcggatactt gaagcatcgc
      481 gcgactgccg ggaatcgacg accttgtcgt cgcgcgagcc acacttgggg caacgcatag
      541 tgtcgacacc ctgaatacca caccaaatgt tgtagcgtca acggaacttt accgtctcgc
      601 gaatttaagc cgccaccgct ttgcgcgctg ttggcttctt cgctttgggc ggtttcgcgg
      661 cgaattcgaa accgactttg ccattctcca gttttaagtg cgcggagaac gggcggcctt
      721 ttttcgagac aaacctgggt agcagatcgg tttttcccgt cgtcatcagt ttttgcacct
      781 gctcttttgg aatgtcgcga tgcagaatgc ttttgcttac gcggaagctg catgtcttcg
      841 gcgacgtgac cgcgttttcg catgcgtagg cacgctcgag cacgtaaacc tgtcccttct
      901 ggcaaactgg acagagaccc aacctctcat gtttgtggat gtcgatcttg atcggttgat
      961 cgtgcgcgcc ttcaccgaaa tcgaattcct gtttgaagtc ttcaccgagt ttcacttcgg
     1021 cagtgaactt gcggcccatt ttgctgcgaa aaccttccag cggaccgaca cgaccttcgg
     1081 tcaaaagttt tgcgatctca ttgcgctcga acaagcgacc cgccatcgtc ttccagatca
     1141 gcaatccgca agatcgacat ttaaacgtgc gatactcctc gtcgaacggc ccgccgccgc
     1201 acttcggaca tttcacctcg agcggctcga aattgccggt tacgttgtcg ccttcaaagt
     1261 tcctcgcttt ctcaacaatt tcgcgcgtga actccttaat gtgcgccatg aattccttgc
     1321 gtttcatcgc gcctttttcc atgcgcttga gcttgtattc ccattcaccg gtcatttcgg
     1381 gcgagcagag ctcggtcacg ccgatgccgc gcaagagtgt gatcagcgcg agtccctttg
     1441 ccgtcgtaat cagctcgcgt ccctgccgcg tgacgtagcc gtcgtgaatt aatccttcga
     1501 tgatggccga gcgcgttgcg ggcgtgccga gccctttctg gcgcatcgct tcgcgcaact
     1561 cttcgtcctc gaccagttta cccgcgcctt ccatcgccga aagcagcgtc gcttccgtaa
     1621 aacgagccgg cggtttcgtt tcgttttgcg ttacgtcgat agtgcgaact ttggcctgtt
     1681 cgttaggcgt gatcgcaacc agcgattcct cgccggattc acccgaaact gcttccctgc
     1741 cgtagaccgc caaccacccc ggatctttga tgatcttgcc ttcggttttg aacgcatcct
     1801 tctccacgcg cgtaattcgt gtggtcactt caaattgcgc tgccgggaag aacacactga
     1861 tgaaccggcg cacgatcatg tcgtaaatct tctgctgcac ttcatccaac ccgtgtggag
     1921 tcgtgccggt cgggataatc gcgaagtgat cggaaatctt cgcgtcattg aaaatccgct
     1981 tggaaagctt cacccaatgt cgtcccaggg cggtgcgcgc gtgcggattg tcgatcttgc
     2041 tcaacgtcgc tttgaccgtc ggcagataat cctcgggcaa aaagcgcgag tcagtgcgcg
     2101 gataggtaat gactttgtgt cgctcataaa gttgctgcgc gatttgcagc gtccttttcg
     2161 cgctgaagcc gcgcgcgttt gcgtcacgct gcaagctcgt caagtcgtac aagagcggcg
     2221 gcgcctgtgt cgttgccttg cgctcttcgg tcgcgacgcc ggttttgccg agacaacgct
     2281 gcttgatctc ctcggcagtt gctttgtccc aaattctctc ggctcgcgcg tcttcgtcgc
     2341 cgtctttggt aaaatcttcg cggaaccagc ggccacgata cgcgccggcg tttacttcga
     2401 aatcgccaaa gacttcgaaa taggtccgcg gcttgaaatc atggatcttg tcctcgcgct
     2461 cagcgagaat cgccagcgtc ggtgtctgca cacgaccgac cggggtgagc tggaaaccgc
     2521 cggtgcgatt gttaaaagca gtcatcgcac gtgtgccgtt gatcccgacg agccagtcgc
     2581 tctcggaccg acagacggcg gcgttggcca ggggaatcat ctcctcatcc gaacgcaaat
     2641 gctgaaacgc ttcgcggatt gaatctagtg tcatcgattg cagccaaagc cggcgcaccg
     2701 gttttttgac gccgctaagt ttcatgaggt agcggaagat caattcccct tctcgtccgg
     2761 cgtcgcaggc gttgatcaga agatcgacat ctttgcgttg tatgagccgc tttagcatgt
     2821 tgaagcgcgc cttcgttttc tcgatgggct tcagctggaa ctcttcggga atgattggca
     2881 ggttggcgaa gctccacttg ccgcgcttta catcgagctc ggttggcagc gccagctcaa
     2941 ccagatgccc aagcgccgag gagatgaggt agcgttcgct ttcgtagtga tcgccctcct
     3001 ttttgaagcc gccgagcgcg cgggcaaggt cgctcgccac gcttggtttc tcagcaataa
     3061 gcagcgcttt agccatggaa ggcggaaatc gaacgccaga tgtcgatgtt ttgcaagtgc
     3121 gagcacttcc cgcgcctaac gagcgagctc gtgccggtgt tcagccgagt ttgacaaaat
     3181 acttgccggg cagctgctta accaatcgct tcagctcgag gctcagcagc gtcgaagaga
     3241 cggcgccgct cggcagcttg cacttagctg agatctgatc gatcggcgtc tcggcagcat
     3301 cgatcgcctc gtagacacgg cgctcctcat ccgacagcgg cggtaacagc cgaacggccg
     3361 cttctggtaa cggctttgcc tccggcaaaa ggatttgtag gtcctcaagg atatcgctcg
     3421 cgtccatgac cagcttcgcg ccttgttgaa tcagccggtt cgagcccatc gcgctcggcg
     3481 cgttgatatg accaggcacc gcgtacactg aacgtccttg ttccagcgcc tgcgccgcgg
     3541 tgatgagcgc gccgctgttt aagcccgcct cgacgacgag aatgccatga ctccacccgg
     3601 agatgatccg gttgcgcatt ggaaatgtgt gccggtctgg ctcgatttcc atcgagaact
     3661 ccgaaacaat agcgccattg ccggcgtgga ttttttcggc gagcgcctgg ttctcaggcg
     3721 ggtacaactt cgctaacccg gagccgatca ctgcgatggt gcggcctttc gcggcaagcg
     3781 cgccctggtg tgcggcagtg tcaatgccgc gcgcgagtcc gctgatgatc gtcaggccgg
     3841 cgtacgctag ttgataggcg agctt
//
LOCUS       DASTDG010000144         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1408733, whole genome shotgun sequence.
ACCESSION   DASTDG010000144 DASTDG010000000
VERSION     DASTDG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408733"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VFO30_04570"
     CDS             complement(<1..1287)
                     /locus_tag="VFO30_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET9418595.1"
                     /translation="MDRIVGIETEYGCLLREEEAHSSSEAWPARVKNYLFRKARAGTI
                     DLHYRDYEEPPGNGGFLLNGGRLYLDMGHIEYASPECLHLRDLVTYDLAGDDLLQSSL
                     IALNAAEHVSFIKNNVDHHTGATFGCHENYLMKREAQFTPPILGTLLTFLATRQIFTG
                     AGRVGQANPLAFDFEPAPQEGQVNFQISQRADHIVNDIYQWVQFNRAIINARDEPLAD
                     YRKYRRLHLLIGDSNMSPFAAALKIGTTACVLSLLEEGRLPRNLVLADAVQSTRDISR
                     DPTRRWIVDLENGKTIGALDVQWQFYELAQRQLRASSAETDWLLESWSFVLEALEQNP
                     DSLIGGVDWITKKWLLEILVEAENLSWDDPWLQSVDLEYHNIDPARGLFFGVTPGKRI
                     GEWNNSVRRIDATHVPPANTRASGRARAVAGFQERDH"
     gene            1416..1871
                     /locus_tag="VFO30_04575"
     CDS             1416..1871
                     /locus_tag="VFO30_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HET9418596.1"
                     /translation="MRELGVRSPYVTVGGIVYFGRMLDKIRAHAKGELPVEYQANLGR
                     GFDASCVKFLQINYEHLVERVKQGGTDEEILQWCFAMGRTPTENDIFVWNEFMRKRGW
                     NDELSDILKRRKKEADMAERSEIQTMFSFIDADEGRAVIGNDKLVPTQH"
     gene            complement(1987..2130)
                     /locus_tag="VFO30_04580"
     CDS             complement(1987..2130)
                     /locus_tag="VFO30_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418597.1"
                     /translation="MTSESIRAWVATVVVILVVIAVWKFIDWRMQPPPPPHPIVAPTP
                     PVR"
     gene            complement(2143..2427)
                     /locus_tag="VFO30_04585"
     CDS             complement(2143..2427)
                     /locus_tag="VFO30_04585"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9418598.1"
                     /translation="MADLIKTGDLKERMKKIPEWELEKKHIERTFEFDDFAEAIDFVN
                     AVAEVAEEEEHHPDIDIRYNKVRLVLSTHSKGGLTDLDFALAERIDTLAE"
     gene            complement(2623..>3811)
                     /locus_tag="VFO30_04590"
     CDS             complement(2623..>3811)
                     /locus_tag="VFO30_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418599.1"
                     /translation="FFLGCGFAAKYLEGGGNFSVPLQWMLGLRRLKLDAVWVELLPAT
                     KNPRADRAKIDNFQRQLRAHGLGRRYCLLYQHPADDGHELDQIRCIGISKRELLDRLA
                     GPNVLLNLSYSIHPPLLLEFERRIFCDLDPSEIFYWMTKLEMGQSYHDEFWTIGLNVH
                     GRDCRLPKTPLVAASLRDARTRPTGPRLQWKTFYPLVDTKLYRQQPRPAIPKFTTIGQ
                     WYWGGTVEVDGEFPDLSKKFAFEQYVDLPSRVTEARFELAMNMSAADPERRRLQKHGW
                     RVVDPHRVVRTPSQYRRYVASALAEFTAIKGVDVAWRTGWLSDRAAAFLASGRPVITE
                     DTGAAKYLPRESGFHFVTSADEAIAAAKSVLADWPRHSKQARRCAVEVFDAPKNLRRL
                     LDL"
BASE COUNT          771 a         1087 c         1154 g          799 t
ORIGIN      
        1 atggtcgcgc tcctggaatc cggcgacggc gcgggcgcgg ccagacgcac gcgtgttcgc
       61 gggcgggacg tgcgtggcgt caatgcgccg gacgctgttg ttccattcgc caatgcgttt
      121 tccgggcgtg acgccgaaaa acaggccgcg cgccggatcg atgttgtgat actcaagatc
      181 gacactttgc agccacgggt cgtcccagct caggttctcc gcttcaacca ggatttcgag
      241 taaccatttc ttcgtgatcc aatcgacccc gccgatgagt gagtcgggat tttgctcgag
      301 cgcttcgagg acaaagctcc agctttccaa gagccagtcg gtttccgcgc tgctagcacg
      361 caactggcgt tgcgccaatt cgtagaactg ccactgcaca tcgagtgcgc cgatggtttt
      421 gccattctca agatcgacaa tccagcggcg tgttgggtcg cgtgaaatat cgcgcgtgct
      481 ttgcacagca tcagcgagga ccaggttgcg cggcaatcgt ccttcctcga gcagcgagag
      541 cacgcacgcc gtggtgccga tcttgagcgc ggccgcgaac ggactcatgt tcgagtcgcc
      601 gatgagcaaa tgcagccgcc ggtatttgcg gtagtcggcc agcggctcat cgcgtgcgtt
      661 gatgatggcg cggttgaact gcacccattg gtagatgtcg ttgacgatgt gatcggcgcg
      721 ctgactgatt tggaagttca cctgcccttc ctgcggcgct ggctcgaaat cgaacgcgag
      781 cgggttcgcc tgccccacgc gcccggcgcc ggtgaagatt tggcgcgtgg cgagaaatgt
      841 gagcaatgtg cccagaatcg gcggagtaaa ttgcgcctcg cgtttcatca ggtaattctc
      901 gtggcagccg aacgtggcgc cggtgtgatg atcgacatta ttcttgatga acgagacgtg
      961 ctcggcggcg ttcagcgcga tgagtgacga ttgcagcaaa tcgtcgccgg cgaggtcgta
     1021 agtgacgagg tcgcgcaggt gcaaacactc gggcgaggcg tactcgatgt ggcccatatc
     1081 gagatagagc cgcccaccgt taagaagaaa acctccgttg ccgggtggtt cttcgtagtc
     1141 gcggtaatgc agatcgatcg tcccggcgcg cgctttgcga aagaggtaat tttttacccg
     1201 cgcgggccaa gcttccgagc tgctgtgcgc ttcttcctcg cgcagcaggc agccgtattc
     1261 agtctctatg ccgacaatcc ggtccattga agccaggtta acgggttact aagcagcgat
     1321 ttgaaagcca cttaaccact gaatcgggca aatctctaag gctatttcgc gctgcggttg
     1381 aaagcgcggc gaaaaagacg cagaatccgt gcgtgttgag agagttgggg gtgcgcagcc
     1441 cgtacgtaac ggtcggaggc atcgtctatt tcggacgcat gctggataaa atccgcgcgc
     1501 acgcgaaagg cgagttgccg gtcgaatatc aggcaaacct cggccgcggt ttcgacgcaa
     1561 gctgcgtgaa gtttttgcag atcaattacg agcacctcgt ggagcgcgtg aaacagggcg
     1621 gcaccgatga ggagattttg cagtggtgtt tcgcgatggg tcggacacca accgaaaacg
     1681 acatctttgt ctggaacgaa ttcatgcgga aacgcgggtg gaacgatgag ctatcggaca
     1741 ttctcaagcg ccggaaaaag gaagccgaca tggccgagcg ctctgagatt cagaccatgt
     1801 tcagtttcat cgatgctgac gaaggtcgag ccgtgatcgg caatgacaaa ctcgtgccga
     1861 cgcagcacta aacgggtaag ggtcagcacg gtgcgctgac cgcggatatc gggtgtgagg
     1921 tggcgatgtt gttagtgtga tcatgccgtc atttaccagc gcgctcggag atcgcgatcc
     1981 acctattcac ctcaccggcg gtgtcggcgc gacgatcgga tgcggcggtg gcggcggttg
     2041 catccgccaa tcgatgaatt tccagacggc gatcaccaca aggatgacga ccacggtcgc
     2101 gacccaggcg cgaatcgatt cgctcgtcac ttgtccctgt cgctactcgg caagggtgtc
     2161 gatccgctcg gcgagcgcaa aatcaaggtc agtgaggccg cctttgctat gggtcgaaag
     2221 caccaggcgc accttgttgt aacggatgtc gatatccgga tggtgttcct cctcctcggc
     2281 gacttcagcg acggcgttca cgaaatcgat cgcctcggcg aaatcatcga actcgaaggt
     2341 gcgttcgatg tgcttcttct ccaactccca ttccgggatc tttttcatcc gctccttgag
     2401 atcgcctgtc ttgatcaggt cagccatagc ctcgaacgac taacacccga aaatagtttt
     2461 gcaatggcaa atcgagggtt cttctccgcc ggtagcaaca acggccgcaa ggtggatcgg
     2521 gcgctccgcg cgcgacgctg tgagtgcgcc tctggcgcca gatttttacc atcgagcaag
     2581 gggctgctcg atccacctgt ctacttcgaa cacgggttga aattaaagat cgagaagccg
     2641 gcggaggttt tttggcgcat caaacacctc aacggcgcag cgccgtgctt gttttgagtg
     2701 ccgaggccaa tcggcgagca cgcttttggc ggcggcgatt gcttcgtctg cgcttgtcac
     2761 aaaatgaaat ccgctctcgc gcgggagata tttcgccgcg ccggtgtcct cggtgatcac
     2821 cggtcggccc gatgcgagga acgctgccgc ccggtcgctc aaccaaccgg tgcgccaggc
     2881 cacgtcgacg cctttgatcg cggtgaactc cgcgagcgcg ctcgcaacgt agcgccgata
     2941 ttgggatggt gtgcgcacga cgcgatgcgg atcgacaacg cgccagccgt gtttttgcag
     3001 gcgccgtcgc tccgggtcgg cggcgctcat gttcattgca agctcaaacc gcgcttcggt
     3061 cacgcgcgac ggaagatcga catattgttc aaacgcgaac ttcttcgata gatccgggaa
     3121 ctctccatca acctcgaccg tgccgcccca ataccattgg ccaatcgtgg tgaactttgg
     3181 aatcgctggt cgcggctgct gccgatacag cttcgtgtcc accagcggat aaaatgtttt
     3241 ccactgtagc cgcggccctg tgggccgcgt gcgcgcgtcg cgcagcgacg cggctacaag
     3301 tggtgttttt ggcagccggc aatcgcgtcc gtgcacgttg agcccgatgg tccaaaactc
     3361 gtcatgatac gattggccca tctccagttt ggtcatccag tagaagatct cgctcggatc
     3421 gagatcgcaa aaaatgcgcc gttcaaactc gagcagcagc ggcggatgaa ttgagtagct
     3481 gagattcagc agcacgttcg ggcccgccag ccgatccagc agctcgcgtt tcgagattcc
     3541 gatgcagcga atttggtcga gctcgtggcc atcatccgcc gggtgctggt aaagcaggca
     3601 atagcgcctg ccgagtccat gcgcgcgcaa ctgtcgctgg aaattgtcga tcttagcgcg
     3661 atcagcgcgt gggtttttcg tcgccggtaa taactccacc cagactgcat cgagctttaa
     3721 tcttcgcagg cccagcatcc attgcagcgg gacggaaaaa ttgccgccgc cctcgagata
     3781 cttcgccgca aacccgcagc cgaggaagaa c
//
LOCUS       DASTDG010000145        12862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1494286, whole genome shotgun sequence.
ACCESSION   DASTDG010000145 DASTDG010000000
VERSION     DASTDG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494286"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFO30_04595"
     CDS             <1..392
                     /locus_tag="VFO30_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET9418600.1"
                     /translation="LGRSMIVGKPLALLLMQKAKGADATVTVVHSRSRKLTEITRSAD
                     MLIAAIGRANFVKAEHVRDGAVVIDVGINRVEDAATERGYRLVGDVDFESVAPKAAAI
                     TPVPGGVGPMTIAMLMCNTVKAARQLP"
     gene            complement(427..1512)
                     /locus_tag="VFO30_04600"
     CDS             complement(427..1512)
                     /locus_tag="VFO30_04600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9418601.1"
                     /translation="MEVVDVAIVGAGPAGSSCAAFCAAAGLRTIVLEREKFPREKVCG
                     DCLNPACWPVLRRLDLFEPVRALPHGRLDCVEFIAISGAKVHVDLPRGEESEIAVKRS
                     LFDELLLTRARELGAAIREQTIVTAIEKTRWGDWRIDIVQDAVRAHALVAADGRNSTV
                     ARLRNLLPPPQRERVALQAHIPLPADFGNRVVLQFLPEGYSGQAPVNENELNICLVGR
                     PDSIRRLKAWAEKTFDLAGENNWRTITPLTRAPIQPAYDNLFFIGDAARVVEPFTGEG
                     IYYALRSGELAANAIIGLLRRQDRQSVVREFTRAHAKMYRGRLWINRLARAAVLSPRI
                     ASAFVHAARIQPALLRLLARKIVATAL"
     gene            1632..2714
                     /locus_tag="VFO30_04605"
     CDS             1632..2714
                     /locus_tag="VFO30_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9418602.1"
                     /translation="MSWSVPILRVAGIQLRIHITFLLLIGWLAIGYYAEGGSAAAAGR
                     VAFILLLFLCVVLHEFGHALAAKSFGINTPDITLLPIGGVARLERMPEEPKQELIIAL
                     AGPAVNVVIALGLMIAIDWHRAAASSVVEASALPAKLLAINVMLLLFNLLPAFPMDGG
                     RVLRALLATRLTYARATQIAASIGQGCAFVFGFIGLLWNPFLLFIALFVYIGASQEAA
                     LAQMKDVSRRFPVASAMVREFRSLPITATLQEAVDALLATSQHDFPVVDEKGGVAGVL
                     TRHDLISALRKDDPAVRVGDVMRRDIPTVTTGTRFEEAFRIMQECNCPALPVLDSMKR
                     LVGLLTPENVSELMMVQSALPRRRAV"
     gene            2747..4381
                     /locus_tag="VFO30_04610"
     CDS             2747..4381
                     /locus_tag="VFO30_04610"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9418603.1"
                     /translation="MPYNSRSAIVDLPGMVVSKKEKPDLRKKVHEVPHKPGVYLMRDR
                     FNRVIYVGKARDLRKRVSSYFLPSKLAVADIKTRAMLDATWDFETHVVRSEAESILLE
                     GKLIKEYRPRYNVSFRDDKKFLLVKVDLSEEWPRFRLTRFKKDDGARYFGPYAHAGAL
                     RQTLNFMRKKFGVLTFGRGAPSERELKSATYQVPTRLSETTAEQYRERVLRAAEFLEG
                     HSREMIATLEEEMRKAADRMDFEKAAELRNMAEDLRATTRPIRRFTRGSLPSAIDPMA
                     DVQALADALQLPRLPRVMECFDISNISTTHVVASMVCFRDGLPDKNCYRRYRVRTVVG
                     QDDFASMAEVVRRRYSRILLAARQANIDAANFSQENPNDALARLQCGATFEAANQSNA
                     QVGNLRHIRLPDLIIVDGGKGQLSAACRELQRLGLHDLPIIGLAKEHEEIYRLGRALP
                     LRLPPNSGALHLLQRIRDEAHRFANAYHQLLLKKRVEESILDDCPGVSQNRKNLLLRK
                     FGSVNRLRRATVEEIAATEGIGRKLAEEVHRFLRQH"
     gene            4401..6287
                     /gene="mrdA"
                     /locus_tag="VFO30_04615"
     CDS             4401..6287
                     /gene="mrdA"
                     /locus_tag="VFO30_04615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9418604.1"
                     /translation="MVRGVIGICWLSILAMPCALFAQTASPSASASPEEETIIPTFET
                     QKLARTYILDVPAPRGQITDRYGTPLAQNKLSYNLAINFPTPLDFSDAQAVAYAREKI
                     DKAQKLIGRTNKISDDAILRHYRNRGIMPFEIAQNLSPSESKGAKDDLPSGMVLRPVY
                     VRVYPNGKLAGQIVGYTGRTGRNPDGIVDNHETLWPETEGREGLEQTFNEMLSGKHGE
                     FKLTFDKDGRKTSEKLVTSPIPGDTVVTTLDLRLQELAEKTLEAKAKRGAIVIVDPNS
                     GDVLALASWPTYDPNTFVPSISPEKFKALQDDPNIPLLPRAFRSAYPPGSTFKVAVGI
                     AALETGAVQPDDRYQCVPVLQIGNVTFHNWKKGDRGALDFVQALTESCDTWFYQVGIK
                     TGAEPIVDWARKLGFGAKCGIPLRGETEGRVADDDYMKATHGRKFLNGDIANLSIGQG
                     DTLVTPLQMAQAMALIANGGTLYQTRLVQQVQTIDNQIVTAYQVRAKRELEISQTTLD
                     ELRTAMVAAVNGANGTAHAASLDNVDVAGKTGTAQWGPKNKERTAAWFAGFLPAEQPQ
                     YAFAAVYEGEVGSKIHGGTTAAPMIAQVFKEMYKGQRLVGNRAREPEPQVRRALPVEE
                     DDSD"
     gene            6351..7412
                     /locus_tag="VFO30_04620"
     CDS             6351..7412
                     /locus_tag="VFO30_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9418605.1"
                     /translation="MSLRKALIVIALGWLALITLLHAAINLHFFGSRRVSATEDKKFR
                     IGFLPVTCHLTCPVTDFINRQMTGDSIFEPVRFNGWPELKEAYLSGYTPATFILAPMA
                     MALREQGVPIKIVYLGHRDGSAVMVHKDSQIYRMQDLRGKKIAVPNRFSNQRLLIFRA
                     LKNAGMSPQDVDLVEMPPPDMPAALYAKAVDAISSGEPFMAQSELDGYGRVLWLTKDV
                     WPNFISCVLAVHEDMIRNRRETVQQLVNGIARSGKWIDRSMEHRMETAQFVSRFYYNQ
                     DPRLLSFVLSKPPDRVKYTNLALRRPDFEEIEQLGRAAGILNGTAHFEDYTDASFVSD
                     ESAIQPYAWEPNQHESRRR"
     gene            7510..8241
                     /locus_tag="VFO30_04625"
     CDS             7510..8241
                     /locus_tag="VFO30_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9418606.1"
                     /translation="MAALFLTGWGLAVRWSHSDLFPTPGNVARGIVDLMQKGLLFKYI
                     VASLFRVSWGFTLAVIIGVPLGLLLGWYARAFQAFNPLIQVFRPISPIAWIPVAILWF
                     GVSDAAPIFLIFLASVFPITVATVAAVQNMQLVYLRAAQNFGLSTGELFRQVIFRACL
                     PQIITGIRIALGVAWLVVVAAEMIAVNSGLGYLIIDARNAGKRYDLVVAGMVMIGVIG
                     LILDVLVRQLEKFDEVRWGYAGRRK"
     gene            8238..9014
                     /locus_tag="VFO30_04630"
     CDS             8238..9014
                     /locus_tag="VFO30_04630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9418607.1"
                     /translation="MRDSAVAAYNLSMRFPGKRRGEEIEVLENVSIDVAKGEFVCIVG
                     PSGCGKSTLLNIVAGFLEPTSGSVIVEGEPVHGPDPRRIFVFQENGVFPWLNVRENVG
                     FGLRKQKQTERDATIARYIDMVGLGGFENAYPHELSGGMRQRVEIARALAARPDIIYM
                     DEPFGALDFLTRLKMRADLIRIWQSEKKTILFVTHDIEEAVQLSDRVFVMTPRPATIQ
                     EIVEVDLPRPRHLDSPDYLAKRDRIFSVMGMSLRVGETAA"
     gene            complement(9053..10309)
                     /locus_tag="VFO30_04635"
     CDS             complement(9053..10309)
                     /locus_tag="VFO30_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9418608.1"
                     /translation="MANWTFTAAPPTEPDQPGEAFVASPVVSNGVVYIGANTGIFYAL
                     DESSGLELWHRSLGYSPHKTCPGRGISSTATVGRDASRGGERTVYVAGGNGYLYALRA
                     SDGVKVWRSFVVNTGTTENTGYNWASPTVINGHVYMGISSDCDDPLIRGGIKDFDQAT
                     GALLHTYWSVPKGSLGASVWTTPASDGKKVWAVLGNGDSGDSFAIVRLDAATFTREDS
                     WVVPDTTGTDLDWGSSPTLFHANVNGTLTKMVGAASKNGKYYAFKAFDLASGPVWGRE
                     LGVPGDPGALKTTGLLLAAAIWDLQGKRLFVGSNETTIAGVTTAGSMRRLDRATGNVI
                     WETPVSGGPIMGSPSMSAGGVIAAATYNTENPSLNQVYLLNASDGSVLNAIQQNAPIF
                     AQPVFADTHLFVATTSATLTAYSTTP"
     gene            complement(10639..10711)
                     /locus_tag="VFO30_04640"
     tRNA            complement(10639..10711)
                     /locus_tag="VFO30_04640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10676..10678),aa:Thr,seq:tgt)
     gene            complement(10811..11509)
                     /locus_tag="VFO30_04645"
     CDS             complement(10811..11509)
                     /locus_tag="VFO30_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418609.1"
                     /translation="MMPQENGQPFIIRSYRASDREAVRKLCCETGFLGVPIDPVYEDR
                     QLFADFLTTYYTDHEPESCFVLEVNSELRGYLLGSRRPLQNQLYSFSQNIILFFRALL
                     RYPRYHSRSRRFIRWLLMYGWREVPAAPRRVPHFHVNLLPDARGVSTTRALMSAYLTY
                     LYRRGDKRVYGQIVTFESRRGEKMFERYGFKVLNRAEITKYKAFYPQSVYLSTVIKNL
                     ETAKPLTVTTRSRS"
     gene            complement(11518..12684)
                     /locus_tag="VFO30_04650"
     CDS             complement(11518..12684)
                     /locus_tag="VFO30_04650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418610.1"
                     /translation="MKICDVTQFYSPLSGGVKRYVHEKAAYIDRYAAEHELVLIVPGA
                     QTNVRSVGRTRIYSLHSPLISRRSHYRALLNLRAIDEIIARERPDVIESSDPYQVAWK
                     AIRAGRVLGIPVIGFYHSHFPEAYVRAATKRFGERISRAALSFSKRYVRDLYNQFVVT
                     IVASEGLANVLREWGVKNVRVVQLGVDTDIFCRRTEGRAVARASLGVATDQKLLLYVG
                     RLSKEKNAALLFDTFELLHRRSPGDFHLIVIGDGSARARLTEAQKRTGGEITWIQYCA
                     ESSELAQYYRAADLLVHPGVQETFGLVALESQACGTPVVGIRGSRMDAVILHDQNSWA
                     GENTPEALADAIETMSARDLPILGRAAARLANERHTWRQVFAQLFCIYREVCAE"
     gene            complement(12671..>12862)
                     /locus_tag="VFO30_04655"
     CDS             complement(12671..>12862)
                     /locus_tag="VFO30_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418611.1"
                     /translation="VEEEADESAFWLELIVEGGLLQEKQVQSLLNEAGELVAITASSK
                     KTAGRTRSQIANRKSREDL"
BASE COUNT         2705 a         3786 c         3745 g         2626 t
ORIGIN      
        1 ttctcggccg cagcatgatc gttggaaaac cgctcgcgct tctgctcatg caaaaggcca
       61 aaggcgccga cgcaacggtt accgtcgttc attcgcgcag tcgcaaactc accgagatca
      121 cacgctcggc tgacatgctg atcgcggcga tcggccgcgc taatttcgtc aaagccgagc
      181 acgtccgcga tggcgccgtc gtcatcgatg tcggcatcaa tcgtgtcgaa gacgccgcaa
      241 ctgagcgcgg ttatcgcctc gtcggcgatg tcgattttga aagtgtcgcg ccgaaagctg
      301 cggccattac gcccgtgccg ggcggcgtcg ggccgatgac aattgcgatg ttgatgtgca
      361 acaccgtcaa ggccgcgcga cagctaccgt agccacgcgc atgtggcgcg cctcgggtca
      421 cacggctcat agagccgtgg ctacaatctt tcgcgcgagc agtcgcagga gagccggttg
      481 aattcgcgcc gcgtgcacga acgccgatgc aattcgcggt gacaagaccg ccgcgcgcgc
      541 aagccggtta atccacagtc ggccgcgata catcttcgcg tgggcgcgcg tgaattcgcg
      601 cacaaccgat tgccgatctt gccgacgcag aagaccaatg atcgcgttcg cggccagctc
      661 gccggagcgc agcgcgtaat aaatcccctc gcccgtgaat ggctcgacca cgcgcgccgc
      721 gtcgccaatg aaaaacagat tgtcgtaagc aggttgaatc ggtgcgcgcg tcagcggcgt
      781 gatcgtgcgc cagttgtttt cgccagcaag gtcgaacgtt ttttctgccc aagcttttaa
      841 ccgccggatc gagtctggtc ggccgacgag gcaaatgttc aactcgtttt catttaccgg
      901 cgcctggccg gaataacctt ccggcaaaaa ctgcaagacg actcgattgc cgaagtcggc
      961 cggcagcggg atgtgagctt gcaaggcgac acgctcgcgc tgtggcggcg gcaggagatt
     1021 acgcaatcgc gccacggtcg agtttcgtcc gtcggcggcg acgagcgcgt gcgcgcgcac
     1081 cgcgtcctgc acgatatcaa tcctccaatc accccagcgc gtcttttcga ttgcggtgac
     1141 aatcgtttgc tcgcgaatag cggcgccgag ttcgcgagcc cgcgtcagca acagctcgtc
     1201 gaacaaactg cgcttgactg cgatctcgct ttcctcgccg cggggaagat cgacatgcac
     1261 tttcgcgccg ctgatggcga tgaactcgac gcagtcgagc ctgccatgcg gaagggcgcg
     1321 caccggctcg aacaaatcga gtcgccgcaa caccggccag cacgccgggt tcaggcaatc
     1381 gccgcagact ttttcgcgcg gaaatttctc tctctcaagc acaatggtgc gcaatccggc
     1441 tgccgcacaa aaggcggcgc agctcgaccc agccggtccg gcaccgacaa ttgcaacgtc
     1501 gaccacttcc atcggagagt tggaataatg gagtagtgca gtaatacaaa ctttccaaca
     1561 ctccacgact ccattactcc cgtgccggat accgctggcg cgcgttctta tttcagacga
     1621 atctcatcgg tatgagctgg tcggtcccga ttttgcgcgt cgccggaata cagctcagga
     1681 ttcacatcac gtttttgctg ctgatcggct ggctggcgat cggctattac gccgaaggcg
     1741 ggtccgcagc cgcagccggc cgggttgcgt tcattcttct gctgtttctg tgcgtggtgt
     1801 tgcatgagtt cggccatgcg ctcgcggcaa aatcgttcgg gatcaatacg cccgacatta
     1861 cgctgttgcc gatcggcgga gtggcgcggc tggagcgaat gccggaggag ccgaagcagg
     1921 aattgatcat cgcccttgcc ggaccggcgg tgaacgtcgt gatcgcgctt gggttaatga
     1981 ttgcgattga ttggcatcgt gccgcagcaa gctcagtcgt cgaagcgtca gcgttgccgg
     2041 caaagctctt ggccatcaac gtcatgctct tgctgttcaa tctgctgccg gcattcccga
     2101 tggatggagg ccgggtcttg cgcgcgctgc tcgcgacacg tttaacctac gcgcgcgcca
     2161 cgcaaatcgc cgccagcatc ggccagggtt gcgcctttgt gttcggattc atcgggctgc
     2221 tctggaatcc attcctgctt ttcattgcac tgttcgtgta catcggcgca tcgcaggaag
     2281 ccgcgctcgc gcaaatgaaa gatgtgtcgc gtcgttttcc ggtcgcgagt gcgatggtac
     2341 gcgagtttcg atcgttgccg attaccgcga ccttgcagga agcagtggat gcgttgctcg
     2401 caacttcgca gcacgatttc cccgtcgtcg atgaaaaggg cggcgtcgcg ggcgtgctga
     2461 cgcgacacga tctgatcagc gcgttgcgca aggatgatcc ggcggtccga gttggtgacg
     2521 tcatgcggcg cgacattccc accgtgacca ccggcacgcg gttcgaggaa gcttttcgca
     2581 tcatgcagga gtgcaactgt ccagccctgc cggtgctcga cagcatgaaa cgactcgtcg
     2641 gtttgctcac gccggaaaac gtgagtgaat tgatgatggt ccaatcggcg ctgccgcggc
     2701 ggcgagcggt ttagcttacc gcaattttag atggcaagcg ggacgcttgc cctacaattc
     2761 tcgcagcgcg attgtcgatc ttcctggcat ggtcgtatcg aaaaaagaga agccggattt
     2821 gcgcaagaag gtgcacgaag tgccgcataa gcccggggtg tatctgatgc gcgaccgttt
     2881 caatcgcgtg atttacgttg gcaaagcgcg cgacctgcgg aaacgcgtga gctcgtattt
     2941 tctgccatcg aagctcgcgg tcgctgacat caagacgcgc gcgatgctgg atgcgacgtg
     3001 ggattttgag acgcacgtcg tccgcagcga agcggagtcg attttgctcg aaggcaagct
     3061 gattaaggaa taccggccgc gctacaacgt ttcttttcgc gacgacaaaa agtttctgct
     3121 cgtcaaggtc gatttgtcgg aggaatggcc gcgtttccgg cttacgcggt tcaaaaaaga
     3181 tgacggggcg cgctatttcg gtccctacgc gcacgcgggc gcgctgcgac aaacattgaa
     3241 tttcatgcgc aagaaattcg gcgtcctcac gttcggccgg ggcgcgccaa gcgaacgcga
     3301 attgaaatca gccacgtatc aggtcccgac gcggctcagc gaaacgaccg cggaacaata
     3361 tcgcgagcgc gttttgcgag cggccgagtt tctcgagggc cattcgcgcg agatgatcgc
     3421 cacgctcgag gaggaaatgc gcaaagcggc cgatcgaatg gatttcgaaa aggcggccga
     3481 gctgcgcaac atggcggaag acctgcgcgc gacgacccgc cccatccggc gattcacgcg
     3541 cggcagtctg cccagtgcaa ttgatccaat ggcggacgtg caggcgcttg ctgacgcgtt
     3601 gcagctaccg cgtttacccc gcgtgatgga atgcttcgat atctccaaca tctccacgac
     3661 gcacgtggtc gcctcgatgg tctgtttccg tgatggcctg ccggacaaaa attgctaccg
     3721 gcgttatcgc gttcgcactg tcgtgggcca ggacgatttt gcgagcatgg ccgaggtcgt
     3781 taggcggaga tactcgcgga tcttgctcgc cgcgcgtcag gcgaatattg atgccgcgaa
     3841 tttctcgcag gagaatccaa atgatgcgct tgcccggttg caatgtggcg caactttcga
     3901 agctgcgaac caatcgaacg cgcaagttgg aaacttgcgc cacattcgtt tgcctgacct
     3961 gatcatcgtt gatggcggca agggacagct ctccgccgcg tgccgcgagc tgcagaggct
     4021 gggactacat gatttaccga tcatcggttt ggccaaggaa catgaggaaa tttatcggct
     4081 gggccgggcg ttgccgttac gcttgccgcc gaattcgggc gcgctgcatt tgctccagcg
     4141 gattcgcgat gaagcgcacc gtttcgctaa cgcctatcac cagctccttt tgaaaaagcg
     4201 ggtcgaggaa agcattctcg atgattgtcc aggtgtgagc cagaatcgaa aaaatctcct
     4261 tctgcgaaaa ttcggatcgg tgaatcgact gcgtagggcg accgtggagg agattgcggc
     4321 gacagaaggg atcggtcgca aactcgcgga ggaagtgcac cggttcttgc ggcaacacta
     4381 gtaatccgca cattcggcag atggttcggg gcgtcatcgg aatttgctgg ttgtcgatct
     4441 tagcaatgcc atgcgcgctg tttgcgcaga ccgcgtcccc gagcgcgagc gcttcgccgg
     4501 aagaagaaac aataatcccg acgttcgaaa cgcagaagct tgcgcgcacg tacattctcg
     4561 acgtgccggc gccgcgcggc cagatcactg accgctacgg cactccgctc gcgcagaaca
     4621 agctcagtta caacctcgcc attaattttc cgacgccact cgatttttct gacgcgcagg
     4681 cggtcgcgta tgcccgggaa aagatcgaca aagcgcaaaa gttaatcggg cggacgaata
     4741 aaatttcgga tgacgcgatc ctgcggcatt accggaatcg cgggatcatg ccgttcgaga
     4801 tcgcgcaaaa tctttcacct tccgaatcca aaggagcaaa agatgatttg ccgtccggga
     4861 tggttttgcg gcccgtttat gtgcgcgttt atccgaatgg caagttagcc gggcaaatcg
     4921 tcggctacac cggccggacg ggtcgcaacc cggatggcat cgttgacaat cacgagacgc
     4981 tttggccgga gacagaaggc cgtgaaggac ttgagcagac gttcaatgaa atgctcagcg
     5041 gcaaacatgg cgagtttaaa ctcaccttcg acaaagatgg gcggaagacg tcggaaaaac
     5101 tggtgacatc tccgataccg ggggacacgg tcgttacgac gctggatctt cgcctgcagg
     5161 agctggctga gaaaacactc gaggcgaagg cgaaacgcgg cgcgatcgtg attgtcgatc
     5221 caaactcggg cgacgttctc gctctggcct cctggccgac gtacgatcca aatacgttcg
     5281 tcccctcgat ttcgccggaa aaattcaagg cactacagga tgatccaaac atcccactgt
     5341 tgccgcgcgc atttcgctcg gcgtacccac cgggctcgac attcaaagtg gcggttggga
     5401 tcgccgcgct ggaaacgggc gcggtccagc cggacgacag atatcaatgc gtgccggtat
     5461 tgcagatcgg gaacgtcacc ttccacaatt ggaaaaaggg ggaccgcggc gcgctcgatt
     5521 tcgtacaggc gctgacggaa tcgtgcgaca cctggtttta ccaggtcggg atcaagaccg
     5581 gcgcggagcc gatcgttgac tgggcgcgca aactcggctt cggcgcaaaa tgcggcatcc
     5641 cgttgcgcgg cgaaaccgaa ggccgcgtag cggacgatga ttacatgaaa gcgacgcacg
     5701 ggcgcaaatt cctgaacggc gacatcgcga acctatcgat cggccagggc gacaccctcg
     5761 tgaccccatt gcaaatggcg caggccatgg cgttgatcgc aaacggcggc acgctttatc
     5821 agacgcgcct cgtgcagcag gtccagacca tcgacaatca gattgtcacc gcctatcaag
     5881 tgcgagcaaa acgggaactc gaaatttcgc agaccacact cgacgaattg cgcaccgcca
     5941 tggtcgccgc agtcaacggc gcaaacggca ccgcgcatgc cgccagcctg gataatgtcg
     6001 atgtggcagg caaaaccggc acggcgcagt gggggcccaa gaacaaggaa cgcacggctg
     6061 cgtggttcgc cggtttttta ccagctgagc aaccgcaata cgcgttcgcc gcagtttacg
     6121 agggcgaggt cggcagcaaa atccacggcg gcaccacagc cgcgccgatg atcgcacagg
     6181 tgttcaagga gatgtacaag ggccaacgcc tcgtgggcaa tcgagcgcgc gagcccgagc
     6241 cgcaggttcg ccgcgcgctg ccggtcgaag aagacgactc cgattagata cgcaccaagg
     6301 ccgaagattg ttgacgcgac gcgcgcacgc tcaatactcg ccttccggca atgtcgctcc
     6361 gcaaagctct gattgtcatc gcgctcggct ggctggcgct cattacgttg cttcacgcag
     6421 ccattaatct tcactttttc ggctcgagac gtgtgagcgc gactgaggac aaaaaattcc
     6481 gcatcggttt tctcccggtc acctgtcatc tcacctgtcc ggtcacggat ttcatcaaca
     6541 gacagatgac cggcgacagc attttcgagc cggtgcggtt caacggctgg ccggaactta
     6601 aggaagcgta tctctccggt tacaccccgg cgacgttcat tctcgcgccg atggcgatgg
     6661 cgttgcgcga gcagggcgta ccgatcaaaa tcgtttattt gggccatcgc gacggttcgg
     6721 ccgtgatggt gcacaaagat tcgcagattt accggatgca ggatttgcgc ggcaaaaaaa
     6781 tcgcggtgcc gaatcgattc tcgaaccagc ggttgctaat tttccgggcg ctaaaaaacg
     6841 ccgggatgtc gccgcaagat gtcgaccttg tcgagatgcc gccgcccgac atgccggccg
     6901 cgctctacgc aaaagcggtc gatgcaattt cgagcggcga gcccttcatg gcccagagcg
     6961 agctggacgg ttatggccgc gtcctttggc tgaccaagga tgtatggcca aacttcatct
     7021 cgtgcgtgct ggccgtgcat gaagacatga tccgcaacag gcgcgagacg gtgcagcaac
     7081 tcgtcaacgg catcgccagg agcggaaaat ggattgaccg aagcatggag caccgcatgg
     7141 agacggcgca attcgtcagc cggttttatt acaaccaaga cccgcggtta ctttccttcg
     7201 tcttgagcaa accacccgat cgcgtgaagt atacgaacct cgccttgcgc agaccggatt
     7261 tcgaggagat cgagcagctc ggtcgtgccg ccggcattct caacggcacc gcgcatttcg
     7321 aggattacac cgacgcgtca tttgtgtcgg acgaaagtgc catccagccc tacgcgtggg
     7381 agccgaacca acatgaatca cgccgccgct gaggtcgcgc ttgatagagg atcgacatct
     7441 agcgccaggc cgaggcgcag cttcggagcc gcggattcgc cgctttggcg aatcgttagg
     7501 ccggtcggga tggcagcgct gttcctcacc ggatggggct tggccgtgcg ctggtcccac
     7561 agcgatttgt ttccgacgcc gggtaacgtc gcgcgcggca tcgtcgacct gatgcaaaaa
     7621 ggattgctct tcaagtacat cgtcgcgtcg ctatttcgcg tttcctgggg atttacgctc
     7681 gccgtcatca ttggcgtgcc gcttgggcta ttgctcggct ggtacgcgcg cgcgtttcag
     7741 gctttcaatc cgctcatcca agttttccga ccgatctcgc ccatcgcatg gatccccgta
     7801 gcgatcttat ggttcggcgt aagcgatgcc gcaccgattt ttctcatttt tctcgccagt
     7861 gtgttcccca tcaccgtcgc gactgtcgcc gccgtgcaga acatgcaact cgtttacttg
     7921 cgcgcggcgc aaaacttcgg cctcagcacg ggcgaacttt tccggcaggt gatcttccgc
     7981 gcctgtttac cgcagatcat caccggcatt cgcatcgcgc tcggcgtcgc ctggctcgtc
     8041 gttgtcgcgg cggaaatgat cgccgtgaac agcggccttg gttatcttat tatcgacgcg
     8101 cgtaacgctg gtaaacgcta cgatcttgtc gtcgccggaa tggtcatgat tggtgtgatt
     8161 ggactcatcc tcgacgtact cgttaggcag ctggagaagt tcgacgaggt tcgctgggga
     8221 tacgccggcc gcagaaaatg agagactcgg ctgtcgcggc ttataatctg tcgatgcgtt
     8281 ttcccggtaa acgccgaggc gaagagatcg aggtcttgga aaacgtgagc atcgatgtcg
     8341 cgaaaggcga attcgtctgc attgtcggtc cgagcggctg cggcaaatca acgctgctca
     8401 acattgttgc cggttttctc gagccaactt ccggctctgt cattgtcgag ggcgagcccg
     8461 tacacggtcc agacccgcgg cgcatattcg tttttcaaga gaacggcgtg tttccgtggt
     8521 taaacgtgcg tgagaacgtc ggcttcggtt tgcgcaaaca aaaacaaacc gagcgcgatg
     8581 cgacaatcgc gcgctacatc gacatggtgg ggctcggcgg attcgaaaat gcttacccgc
     8641 acgagctgtc cgggggcatg cggcagcgcg tcgagatcgc gcgtgcgctc gccgccaggc
     8701 ccgacattat ttacatggat gaaccgttcg gtgcgcttga tttcctcacc cggctcaaaa
     8761 tgcgcgccga cctcatccgg atctggcaga gcgaaaagaa aaccattctc ttcgtcacgc
     8821 acgacatcga agaagccgtg cagctatccg accgcgtgtt cgtcatgacg ccccgccccg
     8881 cgacaattca agagatagtc gaggtcgatc tgccgcggcc acgccatctc gattcgcccg
     8941 attacctggc gaaacgcgat cgaatcttca gcgtgatggg catgagcctt cgcgtcggcg
     9001 aaacggctgc ctaacaaatc ttctacagcg catgagtctc gcgccgcgaa ggctagggcg
     9061 tggtgctata agccgtcaat gtagcagatg tggttgcgac gaagagatga gtgtctgcga
     9121 agacaggttg cgcgaaaatc ggggcgtttt gctggatcgc gttaagcact gatccatcag
     9181 aggcgttcag gagataaacc tggttcagcg aggggttttc cgtattgtaa gtcgcggccg
     9241 caatcacccc gccagcactc atgctcggac tgcccatgat ggggccgccc gaaactggtg
     9301 tttcccagat tacgtttccc gtcgcacgat ccagccgccg catcgagccc gcagtcgtca
     9361 cacctgcgat cgtcgtttcg ttgctgccca caaatagccg tttgccttgc aagtcccaaa
     9421 ttgcggcagc cagtaataag cctgtcgtct taagcgcacc tgggtcgccc ggcactccaa
     9481 gttcgcggcc ccacaccgga cccgaggcga ggtcgaacgc tttgaacgcg tagtattttc
     9541 cgttcttact cgcggcgcca accattttcg tcagcgttcc attcacattc gcgtggaaca
     9601 acgtcggcga tgaaccccaa tccaaatctg ttccagtggt atcgggcaca acccagctat
     9661 cttctcgcgt gaatgtagcg gcatcgagcc gcacgatcgc gaaggaatcg ccactgtctc
     9721 cattgccaag aactgcccac accttcttcc catccgatgc cggcgtcgtc cagacgcttg
     9781 cgcccagcga gccctttgga accgaccagt aggtgtgcag cagtgcgcca gttgcctgat
     9841 caaagtcctt gattccgccc cggatcagcg ggtcgtcaca atccgacgag atccccatgt
     9901 aaacatgacc gttgatcaca gtgggcgatg cccaattgta gcccgtgttc tccgtcgtgc
     9961 cggtattgac cacgaaagag cgccacacct ttacgccgtc gctggcgcgc aaggcataaa
    10021 gatatccatt gccgccggca acataaaccg tgcgttcgcc gccgcggctt gcatcgcgac
    10081 cgaccgtagc agtggacgaa atacctcggc cgggacaggt cttgtgcggc gagtagccca
    10141 acgagcgatg ccacaattcc agacctgagc tttcgtcgag cgcatagaag atcccggtgt
    10201 tcgccccgat gtagacgacc ccgttcgaaa caacggggct cgcaacaaat gcctctcccg
    10261 gctgatctgg ttcggtgggc ggcgccgcgg taaacgtcca attcgccacg accgatccgc
    10321 tcgcggttgg cgtgaacgtc gtcgcgccgt cgttaaaaga agaatgccgc gaagcgtgga
    10381 ggtaacaagg ccaatcgtcc gaagcagcca ggggtaccct cgcagagagg ctgatgaacg
    10441 cgacagtagt gatgactact gcggttctgc gggcccgatc tggcctaagc aatcgctgca
    10501 tgttaacagc gaccgttatt aacagttatt cacaccgcgc gcaagagtta tttccggccg
    10561 ccgcttattt acttcctaaa cggcaattcc gtgtaatctc tgcgcgggta aattgtactt
    10621 cggcgctcaa tgcgagagag ccggcagtcg gattcgaacc gacgacctgc tgattacaaa
    10681 tcagctgctc taccagctga gctatgccgg cgaaaatgcg cgcgcacagt agcgcacagt
    10741 tgtcgatcta acaagcgccc gtggcatggg cttccagccc atgttttatc ggcaagatgc
    10801 cgatgctacg ttacgagcgg gagcgcgtcg taacagtgag gggcttggct gtttcgagat
    10861 tcttgatgac cgtgctgaga tagacagatt gtggataaaa cgctttgtat ttcgtgatct
    10921 cggcccggtt tagaactttg aaaccgtagc gctcgaacat cttttcgccc cgccgactct
    10981 cgaacgtgac gatctggccg taaacgcgtt tgtcgccgcg acgatacaaa taagtcaggt
    11041 atgcgctcat taacgcgcgc gtggtcgaca cgcctcgcgc atccggcagc aagttgacat
    11101 ggaaatgcgg gacgcggcgc ggcgcggcgg gaacctcacg ccagccatac atcaacagcc
    11161 agcgaatgaa acggcgtgag cgcgaatggt agcgtgggta gcgcagtagc gcgcgaaaga
    11221 acaaaataat attctgcgag aaggagtaga gttgattctg tagcgggcgc cgcgagccaa
    11281 gaagatagcc gcggagttcg ctattcactt ctaaaacgaa acaggactcc ggttcgtgat
    11341 cggtgtaata cgtagtcagg aaatcggcaa acaattgtcg atcttcgtaa accggatcga
    11401 ttggcacacc gagaaaaccg gtttcgcagc acaacttgcg aacggcctcg cggtcggatg
    11461 cacgatagct gcggatgatg aacggctgcc cgttctcttg cggcatcatg gacggcgcta
    11521 ttctgcgcag acctcgcggt aaatgcaaaa cagttgtgca aacacttgcc gccaggtgtg
    11581 ccgctcattg gcgagccgcg ccgcggcgcg ccccaagatc ggcaaatcgc gcgcgctcat
    11641 cgtttcgatt gcatcggcca gcgcttccgg agtgttctca cccgcccatg aattctggtc
    11701 gtgtaaaatc accgcatcca tccggctgcc ccggattccc acaaccggtg tcccgcacgc
    11761 ctgactttcc aatgcgacca ggccaaaagt ttcttgcaca ccaggatgca cgagcaggtc
    11821 ggcggcgcga taatactgcg caagctcgct cgattccgcg cagtactgga tccacgtgat
    11881 ctcgccaccc gtccgttttt gcgcctcggt caatcgcgcc cgcgcggatc catcgccgat
    11941 cacgatcaaa tggaaatcgc ccgggctccg gcgatgcagc agctcgaatg tgtcgaacag
    12001 gagtgcggcg tttttttcct tggacaggcg gccgacgtag agcaacagct tctgatcggt
    12061 cgcaacgcct aacgacgcgc gcgccaccgc gcggccctca gttcggcgac aaaatatatc
    12121 cgtatccact ccaagctgga cgacgcgcac gtttttaacc ccccattcgc gcagtacgtt
    12181 cgccagccct tcggacgcga cgattgtgac gacgaattgg ttgtaaagat cgcggacgta
    12241 gcgcttcgaa aaactcaggg cggcacgaga gatgcgctcg ccgaaacgtt tcgtcgcggc
    12301 gcgaacatat gcttccggaa agtgcgagtg ataaaagcca atcaccggaa ttccgagaac
    12361 gcgtccggcc cgaatcgctt tccacgcaac ctgatatgga tcgctcgatt cgataacatc
    12421 cggccgttcc cgagcgataa tctcgtcgat ggcacgcaag ttcaggagtg cccggtagtg
    12481 tgaccggcgc gaaataagcg gcgagtgtag cgagtaaatg cgcgttctcc cgaccgaccg
    12541 cacatttgtt tgcgctcccg gcacgatcag cacgagctcg tgttccgccg catatctatc
    12601 gatgtaagcc gccttttcgt gcacgtaccg cttcaccccg ccactcagtg gggagtaaaa
    12661 ctgcgtcacg tcacagatct tcacgagatt tgcgatttgc aatttgcgat ctcgtcctgc
    12721 cagcagtttt ctttgatgac gctgtgatcg cgactaattc accagcttcg tttaaaagcg
    12781 actgaacctg cttctcctgc agtaagcctc cctcgacgat caactcgagc cagaacgcgc
    12841 tttcatcggc ttcttcctcg ac
//
LOCUS       DASTDG010000146         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_723615, whole genome shotgun sequence.
ACCESSION   DASTDG010000146 DASTDG010000000
VERSION     DASTDG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723615"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFO30_04660"
     CDS             complement(<1..204)
                     /locus_tag="VFO30_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418612.1"
                     /translation="MKTHASPVILDFRPARSLTIAELYGTIGTSLMSEPDLRARIKET
                     LVKNLMLQTTPDEIGDDLPLFGPN"
     gene            complement(251..571)
                     /gene="tatA"
                     /locus_tag="VFO30_04665"
     CDS             complement(251..571)
                     /gene="tatA"
                     /locus_tag="VFO30_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9418613.1"
                     /translation="MILLATFMNLAGPDLIIILLIILVLFGAKKLPELARGMGQAVKE
                     FQKAKDEFSDELHKAGKSDTEAAKPEVKPADATVPRTETAAGTPARPDPNQTITPGDK
                     VDQV"
     gene            684..1562
                     /locus_tag="VFO30_04670"
     CDS             684..1562
                     /locus_tag="VFO30_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9418614.1"
                     /translation="MWKTTHPRDMRPIWKGSISFGLVYIPVAVYPATREEKLSFRQLR
                     STDLSPIRYKKVAEADAKEVAADQIVKGFEYERGRYVVLKDEDFEKVRIESTHSIDIT
                     DFVQLDQVDPKFFYKPYFLEAQKGGEKAYALLHKALSGTGKIGVAKVVIANREYLAAV
                     KPDGLFLILELMHFATEVLTPDELKRPATELSEKELKMAQALIDSMSSDWQPEKYRDQ
                     YRDAVMEIIEQKAKNKELPPPVPAAPRTTNVVDLVKVLQDSLNRTQSLRQRRTGVRSS
                     ARSTTALVKQKRRTAA"
     gene            complement(1585..>2689)
                     /gene="glnA"
                     /locus_tag="VFO30_04675"
     CDS             complement(1585..>2689)
                     /gene="glnA"
                     /locus_tag="VFO30_04675"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9418615.1"
                     /translation="RDPRGVAEKAEAYLQSTGIADTAYFGPEAEFFIFDDVRFSYSGN
                     ASFHRVDSMEGHWNSAREEFPNLGYKIRPKEGYFPVAPMDSLQDIRNEMCLELERAGI
                     PIERQHHEVATAGQAEIDIRFAPLKRMGDNLQYYKYILRNVAKKHNKTVTFMPKPLYA
                     DNGSGMHTHMSLWKDEKPLFAGNGYAGLSDTALFFIGGILRHAPALTCLTNPTTNSYK
                     RLVPGFEAPVNLAYSARNRSAAIRIPTYSAEPKTKRIEFRTPDAAANPYLAFSAMLLA
                     GLDGIQNRMDPGDPLDKNLYELPPEELAEIASVPDSLRGAIDALEADHTFLLKGDVFN
                     ADFIANWIDMKQKEYDALRLRPHPYEFSMYYDV"
BASE COUNT          569 a          707 c          818 g          595 t
ORIGIN      
        1 gttcggccca aagagcggca gatcgtcgcc gatctcgtcg ggcgtggttt ggagcatcag
       61 gtttttcacc agggtttcct tgatgcgggc gcgcagatcg ggttctgaca taagagaagt
      121 gccgattgtg ccgtaaagct ccgcgatggt caaactgcgc gctggccgga aatcgagaat
      181 cactggagag gcgtgcgtct tcacgcgcgc cgtttacgtg cgctgtaaat gcgcgtgccg
      241 agagctggcg ctacacttgg tcgactttgt cgccgggcgt aatcgtttgg tttggatcgg
      301 ggcgcgctgg ggtgccggct gcagtttccg tgcgcggcac cgttgcgtca gctggtttga
      361 cctctggctt cgctgcctcg gtatcagatt tgccggcctt gtgcaattcg tcactgaact
      421 cgtctttcgc cttctggaat tccttcactg cctgacccat tccacgcgcg agctccggca
      481 atttcttcgc gccaaagagc accagaatga tcagcagaat aatgatcagg tccggcccag
      541 ctagattcat gaatgtggcg aggagaatca ttttagctaa tgatcagacc ctttggtgaa
      601 gttgcaaggt aattcgccag atagctagcc aagccgtttc tttgcagcat gcaacctgct
      661 gagcagttga cacatccacc ttgatgtgga aaacaactca cccgcgcgat atgcgtccga
      721 tctggaaagg cagcatcagc ttcggcctgg tttacattcc ggtcgcggtt tatccggcga
      781 cccgggaaga aaagcttagc ttccggcaat tgcgctcaac cgatctgagc ccgatccgat
      841 acaagaaggt ggcggaggcg gacgcaaaag aagtcgcagc cgaccagatc gtcaaaggct
      901 tcgaatacga gcgcggccgt tatgtggtgt tgaaggacga ggacttcgaa aaggtgagga
      961 ttgaatcgac gcactcgatc gacatcaccg attttgtcca actcgatcaa gtcgacccga
     1021 agttctttta caagccgtat tttttggaag ctcagaaggg cggcgaaaaa gcatatgcgc
     1081 ttttgcacaa ggcgctcagt ggcaccggca aaatcggcgt ggccaaagta gtgatcgcga
     1141 atcgggaata cctcgcggct gtaaagccgg acggattgtt tctgattctt gagttgatgc
     1201 atttcgcgac ggaagtgcta acgcctgacg aattgaagag gccggcgacc gagttgagcg
     1261 aaaaggaact gaagatggct caggcattga tcgacagcat gtcgtcggat tggcagccgg
     1321 aaaaatatcg cgaccaatat cgcgacgcag tgatggaaat aatcgagcag aaggcgaaga
     1381 acaaagaact gccgccgcct gttccagccg cgccgcggac gaccaacgtg gtcgatcttg
     1441 taaaagtcct tcaggacagt ttgaaccgga cgcaatcact ccggcaaaga cgcaccggtg
     1501 tgcgatcgag cgctcgctcg accaccgcgc tcgtgaagca gaagcgccgg acggccgcat
     1561 agcgggactc cgccgcacga ccgtttacac gtcgtagtac atcgagaact cgtacgggtg
     1621 ggggcgcagg cgcaatgcgt cgtattcttt ttgtttcatg tcgatccaat tcgcgatgaa
     1681 gtcggcattg aagacgtcgc ctttgagcaa aaacgtgtga tcggcttcga gcgcgtcgat
     1741 cgccccgcgc aatgaatctg gtacgctagc aatttcggcg agctcttcgg gcgggagttc
     1801 gtacaaattc ttgtcgagcg gatcgccggg gtccattcga ttttgaattc catccaggcc
     1861 ggcgagcaac atcgcactga aagcgaggta cggattcgct gcagcgtcgg gcgtgcgaaa
     1921 ttcaattcgc ttcgttttcg gttcggccga ataggtcggg atgcgaattg cggccgagcg
     1981 attgcgcgcg gagtaggcga gattgactgg cgcttcgaat ccagggacga gccgtttgta
     2041 gctattcgtg gtcgggtttg tcaggcaggt gagcgccggc gcgtgacgca aaattccacc
     2101 gatgaaaaag agcgcggtat cgctcaagcc ggcgtacccg ttgccggcga agagcggctt
     2161 ttcgtctttc cacagcgaca tgtgcgtgtg catacccgag ccgttgtcgg cgtagagcgg
     2221 cttcggcata aaggtgacgg ttttgttgtg cttcttggcg acgttgcgca ggatgtactt
     2281 gtagtattgg aggttgtcgc ccatccgctt caacggcgcg aaacggatat cgatctcggc
     2341 ctgacccgcg gtggcgactt catgatgctg gcgttcgatc ggaatcccag cacgctccag
     2401 ctccaggcac atttcgttgc ggatgtcctg caacgaatcc atgggggcga ccggaaaata
     2461 gccttccttg ggccgtattt tgtaaccgag gtttgggaat tcctcgcgcg cgctgttcca
     2521 gtggccctcc atgctgtcga cgcggtgaaa cgacgcgttg ccgctgtagc tgaaccgcac
     2581 atcatcgaag atgaagaact ccgcttcggg cccgaagtag gcggtgtcgg cgatgccggt
     2641 gctttgcagg taagcttcgg ctttttccgc aacgccgcgc gggtcgcga
//
LOCUS       DASTDG010000147        25141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1075036, whole genome shotgun sequence.
ACCESSION   DASTDG010000147 DASTDG010000000
VERSION     DASTDG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 25141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25141
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075036"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFO30_04680"
     CDS             <1..396
                     /locus_tag="VFO30_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418616.1"
                     /translation="AFAYRNLKNFPAAIADYTNVLNQNPNDEQTLAKRGYTYSLMQDY
                     EKAIADYQQALKLKPEDNDTFSRLQYAQSMLAARNAPPPTPTPSPVVKPSVFTPLNIG
                     IALAVLIVIAIIWRLVTRGREEPTSHRIR"
     gene            547..622
                     /locus_tag="VFO30_04685"
     tRNA            547..622
                     /locus_tag="VFO30_04685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Thr,seq:ggt)
     gene            694..1878
                     /gene="tuf"
                     /locus_tag="VFO30_04690"
     CDS             694..1878
                     /gene="tuf"
                     /locus_tag="VFO30_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9418617.1"
                     /translation="MAKEAFKRDKPHVNVGTIGHVDHGKTTLTAAITSVLSKKGMAEI
                     RAYDSIDNAPEEKERGITINTAHVEYQSDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKVDMVDDPELLDLVEMEVRDLLTQ
                     YEFPGDKIPIVKGSALKALNGDAEAEKQVLALVEAIDDYIPVPERPIDQPFLMPIEDV
                     FNIEGRGTVATGRVERGVLKKMEEVELVGIRPTTKTVATDIEMFRKLLDEARAGDNVG
                     VLLRGIKKEDVERGQVIAKPGSITPHKKFKAEVYVLSKEEGGRHTPFFTNYRPQFYFR
                     TTDVTGTVKLPEGVEMVMPGDNINIEVELITPIAMEKTIRFAIREGGKTVGAGRVTDI
                     LD"
     gene            1895..1967
                     /locus_tag="VFO30_04695"
     tRNA            1895..1967
                     /locus_tag="VFO30_04695"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1928..1930,aa:Trp,seq:cca)
     gene            2066..2284
                     /gene="secE"
                     /locus_tag="VFO30_04700"
     CDS             2066..2284
                     /gene="secE"
                     /locus_tag="VFO30_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9418618.1"
                     /translation="MITRTKNFFNEVKVELQKASWPWDPKEKGFRKYKELFDSTGVVL
                     ISMLLLGGFVAFFDFVLVNFVHFFTRLH"
     gene            2288..2857
                     /gene="nusG"
                     /locus_tag="VFO30_04705"
     CDS             2288..2857
                     /gene="nusG"
                     /locus_tag="VFO30_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9418619.1"
                     /translation="MAQTLAGKDQWYVVHVLSGQENKVKENIEKRIKTEEMGDLIYEV
                     LVPTERVSEIKKGKKSETTRKFFPGYLIVNMHLLDENNELVGRTWYFIRDTPGVIGFA
                     GTKDKPIPMRHSEVESMLAQMKEREEKVKPKVSFEVGESVKVADGPFQNQTGIVEEID
                     PERGKLRVSVTIFGRATPVELEYWQVERG"
     gene            2952..3377
                     /gene="rplK"
                     /locus_tag="VFO30_04710"
     CDS             2952..3377
                     /gene="rplK"
                     /locus_tag="VFO30_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9418620.1"
                     /translation="MAKEVVAHIKLQIPAGQANPAPPVGTALGPRGVNIMAFCKEFNA
                     ATQKQAGDILPVVITVFKDKSFTFITKSPPAAVLLKKAANIAAGSKEPNKTKVGKVTR
                     KQVMDIVNTKRKDLNARNDQAAFNIIAGTARQMGLEIVD"
     gene            complement(3426..3797)
                     /locus_tag="VFO30_04715"
     CDS             complement(3426..3797)
                     /locus_tag="VFO30_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9418621.1"
                     /translation="MLREYLPILLQIIVAIVFAASALVVSALIGKTGRRTPAKDTPYE
                     CGMVPGGEGQPRFSVKFYLIAMLFILFDVEIVFMYPWAVVYKDMIAHSAVIFWSMLSF
                     ISILMVGYVYALKKGALDFRK"
     gene            complement(3831..4145)
                     /locus_tag="VFO30_04720"
     CDS             complement(3831..4145)
                     /locus_tag="VFO30_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418622.1"
                     /translation="MAILKKNAFSRRIPDLVTEELVAVLSRRASYEFKELFDIVHENL
                     RARNAASGGEEMLRLRAYEKLQNLVARGAVKKNGKKYKGLPSALASAIAPPNGHMPAA
                     TR"
     gene            complement(4257..5816)
                     /locus_tag="VFO30_04725"
     CDS             complement(4257..5816)
                     /locus_tag="VFO30_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418623.1"
                     /translation="MRDQWHQSDLYQLYREPAVQDFLRKPLDTAPKADAARQAFKDIE
                     QLDPKDAFAALTSIENNKPRLLAGFRFRGRQAAVEQIIGKWRANLLHRNRNATREKTT
                     HGRHEIEAVSAGGMTLATTYAQQWFFAANDVSELEALLDRADAVKQDRQSALQSDEAY
                     RGAVGHMPSEYALLVYLQPKAFAEKLDALRATFGQQISADKPTLIEQIRNICGAIRFE
                     RGKMHDVFFAAMPRQTDAKLTRSTEALGTADTFFYLATLLNPQNLGATNQAAAIAPLG
                     GWLQKFIGVTQKSGITANDWKAAFDLELSALADWPPNARWPSFIATLPVRDSPRAQKI
                     VAAMTAALDEDAAWIKTEKEGVTYFTIEASRAVFAISPTLGLSDRLFVAGLDPSAVTA
                     AIERSQKSNAPLSNSPTYKTAARILPEPTESFAYVDVALLYSRLDAAVRPLLMLGAAF
                     LPAIANDVDLAKLPPQHVITKHLSPIVSSQRYERDGYIAESIGPVTANEAVLVIGLAV
                     VYRGFAGHHAD"
     gene            complement(6012..6902)
                     /locus_tag="VFO30_04730"
     CDS             complement(6012..6902)
                     /locus_tag="VFO30_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HET9418624.1"
                     /translation="MEQALDAFIRFLAVERGLSNNYQLSTQRSLTEFAQWCKAKHRID
                     NPRQVTLAILSDYLAERKRNGLAAASIKLIVVALKIFFRFLAARGEIADNPAEALALP
                     RIERYLPDTINELQVEQLLDSIDTKVPLGLRDHAIVELLYASGLRISELTAARLENFN
                     AQERILRVIGKGNKTRLVPVGRKAAEALAAYLSLERPKLMKRATGSEIFLSARGTRLT
                     TGRVWQIVKQIAQRSGLETNVYPHLLRHSFATHLLGNGADLRIIQEMLGHADISTTQV
                     YTHVDQQRLKAVHRQFHPRA"
     gene            7029..7784
                     /locus_tag="VFO30_04735"
     CDS             7029..7784
                     /locus_tag="VFO30_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET9418625.1"
                     /translation="MSLDLNSLLRDWPHEPGAIKVRKIAGLDGREKLQLRIDLGVLQM
                     EMSGRPDGARPHGFESLLAYHQDRAARAESTGEEYELSAEQCTELQQEGIQYYHRYLS
                     LFQINDFGGVVRDTQRNLDLFDFVIAHSDREELAWGLQQFRPYVLMMNTRAKASIFLG
                     AGKFPEAIREIERGRDAILDFFQRSNFPELAAKSSEIAFLEEWLEEVRAKRPLSKLEL
                     MQREMETAIARELYERAAEIRDAIKLLKTQQSV"
     gene            7993..8883
                     /locus_tag="VFO30_04740"
     CDS             7993..8883
                     /locus_tag="VFO30_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418626.1"
                     /translation="MLERALSDEEHNQILQTIEMFEAITQTQPDDYQSLEILKEAYQK
                     VGRQEDFVRVSRRLAECYFSAGAHSNAMRECEAILLNEPNAPDILAMLGEIETRLQSS
                     GEAIVGAPGNNGALDDTDGSLIDIGSRAGKVGKLANLHERGDDHLAKFLIVQQLFAEE
                     EVSSALETVTLLNKELSGQALAASLLDQLCKADNARLEAVLSALIDRTKFAYVPLEYY
                     DIDRQIAPMLPDYLTVGRLFVPFDLVSRTVMVACCNPFDSTGREAFQQSVDYTVAWYL
                     ARPSAIVKNLQSIYRLDARV"
     gene            8896..10617
                     /locus_tag="VFO30_04745"
     CDS             8896..10617
                     /locus_tag="VFO30_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9418627.1"
                     /translation="MRSKSFAERIADVLIEDGLLLPNQLEEAVNIQKTEGGRLLKILT
                     DRQFVTEQDMAFSMGRCLNTPPVNLSKLRVPEEVMSLVPREMAKANKLVPIARLDGKL
                     FVAMADPTNVLAVDDLKRRVQLEIVPMIATEQAVNDVLSGIHTSSANMSQVLKKVAEE
                     AAQTSEVEVHAAKHEEIDLDRLAADSEDAPVIKIVNLVLVQAMKEKASDIHIEPFQNS
                     LKLRYRIDGELVLAESPPKALQLAITSRIKILAGLNIAERRLPQDGRFRIRVMGKEVD
                     LRISMLPTAHGEKIVIRILDKASLTGGIDQMGIDADTLSKFRKAIDAPHGMILVTGPT
                     GSGKTTTLYSVLHELNNPAYNIVTVEDPIEYELMGINQVAVRSDIGLDFSSALRSILR
                     QDPDIVMVGEIRDNETADIAVKAALTGHQVLSTLHTNDAAGAITRLDDMGIEPFLISS
                     SILMACAQRLVRRICTNCREEFVPELEIFERLGIEQTKGTVFYHGTGCDRCKGRGYLG
                     RVAIVEALPVSEAVRRLIIKRASAAVIKNQAMSEGMKTLRMVGVEKAVEGVTTLEEIW
                     RVTSEDH"
     gene            10619..12589
                     /locus_tag="VFO30_04750"
     CDS             10619..12589
                     /locus_tag="VFO30_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418628.1"
                     /translation="MKIPFSGLYEKLRSRVSAPSAASQRPVVSQPVVKSGTSLSKTVL
                     PNTTRKVAEPDPLQTAAGVLAKAASQSAVRIEAVPFTASTKPRETSERSAVKLEAKTE
                     RAISLCLDDILDRLPPGVVKARESFDPKRHILLKASELEKGMASGKPAVALTNIYQQV
                     PEVFLHAPAATDSTTVYLPFEKVLAQFMSLQVRSDQEHEQVVPHVETPILQVTLEDKD
                     RFGTPIEPLQSSSAPSARAASPTTSSIPAAEAKTTAVATPKPPPIRAPIPLVAKLPLA
                     PATRSQPGEAAAPSVELPPNGTGVPASERVPASSGPPAPITPSSPPPPPIPFKVPPPA
                     SDIRPRFIRVPGVEPSEGASSTQKLTPSRSPDPTIALTLLPILEELPPFQLSGSPSVV
                     PDDVRIRLPLSLIEPQLATGCVTVPAKVLQKAMPAEYRSLIKSDTAETPISLSLSEVL
                     KHVPVALLRRREDQEQPPAVENVETPISIAADEDAKRLAESRQTRSDKRAEGVPIKFE
                     RDTVTAEAKNTEAAEERLEQPEQPLDARTVVARANALPGVAVCAVTFADGLNLAGNLP
                     AELGADGLCALAASLLQKIDNHLHEPKIGPLVGMTLHAAKSITFLRRDNIVLAALHSG
                     DDLPTGTREKLATLVEKLSRTYAESEPAHVHH"
     gene            12576..13247
                     /locus_tag="VFO30_04755"
     CDS             12576..13247
                     /locus_tag="VFO30_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HET9418629.1"
                     /translation="MSIINYASREIQFKVVYYGPALCGKTTNLNYIHQRITPQNRGDL
                     ISLATAADRTLFFDFLPLNAVVIKGFVTKFQLYTVPGQVIYNATRQLVLRSVDGVVFV
                     ADSQWEKMEENVESFKNLADNLAQQNVSIDEVPCVLQYNKRDLPNIAPINYMEYVLNN
                     GKKRLQSFEVISSTGQNVFASLNAVSQTLLHKFSKQDDAPQRAPAPVATPIESPLGRP
                     HSAPV"
     gene            13244..13705
                     /locus_tag="VFO30_04760"
     CDS             13244..13705
                     /locus_tag="VFO30_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9418630.1"
                     /translation="MNAIPVLTIEDVAAVDGVLEDFLRKTEADLTVVIDRGGNVISQF
                     GDMDVTDVTVVAALAAGSFAATRELARRIGELEFNALYHQGNGTHIFMNSVDEDTIMT
                     TVFGRGTTVGLVRFYSAAAAQRLAEILKSLRHKSPTLKLAPVSSATVFADR"
     gene            complement(13772..15022)
                     /gene="fabF"
                     /locus_tag="VFO30_04765"
     CDS             complement(13772..15022)
                     /gene="fabF"
                     /locus_tag="VFO30_04765"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9418631.1"
                     /translation="MNTDRRVVITGLGAVTPLGNDVETFWSNLKNGVSGIRHIEAFDT
                     SEHDCKIGGEVRGFEPKRYFKNAKDVRRTDRFAQLALAAAKMALQDAGIDLERTNRER
                     FGVIVSSGIGGLKTLEDQYRILLTKGPSRTSPFTIPMLISNMASGLISMEYALQGPNL
                     CIVTACATSNNAIGESWRTIKFGDADIFLAGGSEASIVPIGLAGFSAMKALSLRNDEP
                     ERASRPFDRDRDGFVLGEGAGVVVVEELEHARARGAKIYCELSGYGVSADAYHMTAPP
                     PEGEGAARAMQLALEHAGVSPDEVDYINAHATSTGLGDISETQAIKKVFGKKAYDVSI
                     SATKSMTGHLLGGAGAVETVVCALAIRDSVIPPTINLENPDEQCDLNYTPNVAQERTV
                     RVALNNSFGFGGHNAALVTTAFES"
     gene            complement(15109..16281)
                     /locus_tag="VFO30_04770"
     CDS             complement(15109..16281)
                     /locus_tag="VFO30_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET9418632.1"
                     /translation="MWIGRNRKARVTYGFDEIALVPGRVTINPNEVDITFRLPRKDGA
                     PLELKIPILASAMDGVVDVRFAIAMGKLGGLAVLNLEGVQTRYKNPQEVLQKIVEANR
                     SQITGLLQKIYQEPVQEELIAARIRQIKDGGALAAVSCIPQRAEQFGKIAQEAGADVF
                     VVQSTVSTVRHISSEYKSLDLPKFCAEMRIPVIVGNTVGYDVTLDIMECGPAAVLVGV
                     GPGAACTSRGVLGLGVPQVTATVDCAAARDAYFKKTARYVPIITDGGMSKGGDVCKAL
                     ASGADAVMVGSAFAKAQEAPGCGYHWGMATPHVNLPRGTRIKVGTTGPLKQILFGPAE
                     VDDGSQNLVGAITTCMGNVGARDLSEFQQTEIIIAPSIRTEGKLFQAVQSVGMGTK"
     gene            complement(16527..17324)
                     /locus_tag="VFO30_04775"
     CDS             complement(16527..17324)
                     /locus_tag="VFO30_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418633.1"
                     /translation="MNAAAADEFPRLIFDWDPPHRRDFTMLGFVTASFLGHAFCFYLF
                     QIVYPPTIAILPPPARVSVISPSSEEGRNLLYRIEAEDPALAFATQRPPESRLRALPK
                     VEHVPSYLANEPAIQQLPPLIMDLHVPSAQPPGPVSIIHPKPPPRGREFSTTVLFSDE
                     LAAIGAATCSHTKFTVTTHEPPQAVQFRVAVNARGEIQHCFTINSSGDAALDEQARQH
                     LVLCRFPVRSTAGKTDEGSVTWGLATVEWGTDIDRPRANSGAATSAQ"
     gene            complement(17321..17725)
                     /locus_tag="VFO30_04780"
     CDS             complement(17321..17725)
                     /locus_tag="VFO30_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9418634.1"
                     /translation="MKLTRTKEYNFGWLLVFPLLDVLFIIVFFLLLGSNFLLQPGIAV
                     SLPFSRFTLGPQPNQQIVSITGGAVAAIYFRDQKVTVEQLGPLFDAAKRDGTSLVIRA
                     DRLTPYETVAEVANAALEHGIASVALATAPPP"
     gene            complement(17795..18472)
                     /locus_tag="VFO30_04785"
     CDS             complement(17795..18472)
                     /locus_tag="VFO30_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9418635.1"
                     /translation="MIEYIQKGGLLMWPILVCSIISIAVFAERLFYFHRASINVGEFL
                     KGLTNLIQRRNFAEALHESAGTPGPIARVIHAAIIRHDAPRSELREIVQEAAQLEVPK
                     LERYLGVLATLAFLAPLLGLLGTVTGMIDAFGTISSHGGYATVTELSSGIYKSLLTTA
                     AGLVVATPTFVAYSYLSSRVNTMLHEMERAGIEIMHLLTETRPIGGIISFEQPEQRSE
                     AEVRLDR"
     gene            complement(18523..19032)
                     /locus_tag="VFO30_04790"
     CDS             complement(18523..19032)
                     /locus_tag="VFO30_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418636.1"
                     /translation="MSPFARSTIACAAFLAARTIIFGQAGEDSNTPVRLTMSVNQDGS
                     RTVYKFNDAKHTATATATDADGKLREKIHYQLDDSGRFASALVFAPDGKLRFKSRYKY
                     DSAGKLEEETQFSEGDAVLHKIVYNYDGGGKPTGYSVFDADGKLISRVAPLAPATPTS
                     VKPRLKNGR"
     gene            19168..19623
                     /locus_tag="VFO30_04795"
     CDS             19168..19623
                     /locus_tag="VFO30_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418637.1"
                     /translation="MPSPSPTQSFYAGEFRHFIDEKNRITIPSRWRRREGEEFILLPE
                     ASHQFLLVMSPDEFARMSSAAENTSNVSARDRRVFLRQLHSRAQHAAADRQGRLVLPE
                     ELCKQTGLKGEVALVGGRGRFEIWNLQRWKRAHEEETPTYQHVAGVIGL"
     gene            19659..20660
                     /gene="rsmH"
                     /locus_tag="VFO30_04800"
     CDS             19659..20660
                     /gene="rsmH"
                     /locus_tag="VFO30_04800"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9418638.1"
                     /translation="MNALLFERPVWFATGPLVLEEEAQDMEDFTYHRPVLAREVIELL
                     APKPGSLVIDATCGGGGHTEAILAAGADVLALDQDPDAIEYAQKELARFGGRVTLHQA
                     NFRHADKILDELGIAKVGGALVDLGVSSRQLENAERGFSMMRNGPLDMRMDPRTELTA
                     ATIVNNYSEEELTRLFRELGEEPAARRIASIIVKERKTTPFRETLQLARAIEKVVGRH
                     GRRHPATQVFQALRMQVNDELGALPDGLRVMTERLEPGARIAVIAFHSLEDRIVKNFF
                     RDHSRDWLDRPEWPEPRRNPEYDLKIITPKPIEPGEEEQRANPRSRSAKLRVAEKLA"
     gene            20657..21022
                     /locus_tag="VFO30_04805"
     CDS             20657..21022
                     /locus_tag="VFO30_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418639.1"
                     /translation="MNRSRRNWNTVDAASLVRWLVLTAFLALTGLSYVYLTLQLYHLG
                     DRKKALENELVSLRSQNDVVSAQIAALTSRIALQRRLKEGYLKMVPITERNIVRLTVP
                     ARSADEDRVQPVANQTVHR"
     gene            21019..22782
                     /locus_tag="VFO30_04810"
     CDS             21019..22782
                     /locus_tag="VFO30_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9418640.1"
                     /translation="MKWNSRVRCALVGLAFTALFSAFSFRLVYLQIIKHEEYAGLAAE
                     KHGDKQIIRAERGEIFDVNNEVLAHNVPVETVVADGTHLNDRDATVDLVSAELNLPRH
                     EVREKLAGDRRYVVIKRDVPRAVAESLRSQLRARNRRGIYFEHDASRIYPNGSMLCHA
                     IGFTDFEHRGIQGVEASMEEYLRGQDGYRFIEHNCAGQEIVLYRGQERAPRNGYDVHL
                     TVDLHLQDIVENEIDAAVREYHPAKATIILMRPRTGEILAMANRPNFDLNQRSQAAPE
                     QMKNRAIIDLMEPGSTFKIVAAAAALNERTVNPDTEIFCEHGLWNFGGSALHDHRSFA
                     DLSVKDVLIKSSNIGAAKLALGVGEQKFYEYIRRFGFGERTGIELPGEISGMIRSPQS
                     WSKISITRIPMGHEIGVTPLQMTAAMATIANDGKLVAPRIVKSITTPDGKTISSFSPT
                     VVRQVISPQTARQIGDALRGVVSDRGTAAAAAIPGFTIAGKTGTAQKVDPKGGYETGK
                     YVVSFSGYLPAEHPEFVGFVVLDDAHTSKPELNYGGLVAGPIFARIAEKAARYLDLQP
                     REEIRRALPVETVAISKAVAR"
     gene            22834..24315
                     /locus_tag="VFO30_04815"
     CDS             22834..24315
                     /locus_tag="VFO30_04815"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9418641.1"
                     /translation="MQLEKLLRATAVRQVIGPPDRDVESVAYDSRRVQTNSMFVALRG
                     EKTDGHQFIGQAIDHGASVIVAEREEKHPRATCIVVENTRIALADIAVAFYRRPAERL
                     KLAGITGTNGKTTTTFLLKHICEEAGLRCGLIGTVRYEVAERILPATRTTPESLDLQE
                     LLAAIVNAGCRAATMEVSSHALAQERTRGIEWDVAVFTNLSQDHLDFHGTMESYFEAK
                     ARLFTELSAQQKKREPVAVVNIDDPYGEQLLRKIDNTVSVITYGSGVRANFRASSYRA
                     RFGSTSYQLEARGRSYLVRLPLIGRFNVANSMAALAAATALGINLRQAVLSLAKSPQV
                     PGRLEMVPAKRKFQVFVDYAHTPDALLNVIKTLRELEPRRLIVVFGCGGNRDRQKRPL
                     MAEVVDRNADYAIITSDNPRKEDPAAILSEVEKGFRSNHFEKIVDRTQAINRAVKLVQ
                     PRDIVLIAGKGHETYQEFADRTVPFDDIQVARRAIENHPVEFQ"
     gene            24312..>25141
                     /gene="murF"
                     /locus_tag="VFO30_04820"
     CDS             24312..>25141
                     /gene="murF"
                     /locus_tag="VFO30_04820"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9418642.1"
                     /translation="MNRLSILQIAEFADGKLSGDAGSTLVEKISTDSRTLKPGELFVA
                     LHGEKFDGHKFVEAAANAGAAGAIVDVAWTGNAPAKFPLIRVQDTLQAYQNLAAKYRG
                     SLSLRVIAITGSNGKTSTKDFTAAVLARKFRVTKTEGNFNNHVGLPRTILQATSQDEV
                     AVWEIGMNHPGEIAALAKLAAPDVAIITNIGLAHIEFMGSCEAIAREKGALAEAVGAS
                     GSVILNADDPFSKSIAQRTNANVIFAGTSGGTIRANDITQSADGSEFTISEGAHRCRA
                     "
BASE COUNT         5461 a         7287 c         7336 g         5057 t
ORIGIN      
        1 gccttcgctt atcgcaacct gaaaaacttt cccgcggcga tcgccgacta tacaaatgtt
       61 ctgaatcaaa acccgaacga cgagcaaacg ctggcgaaac gcggctacac ttattcgctc
      121 atgcaggatt atgagaaggc gatcgctgat taccagcagg cgctgaagct caaacctgag
      181 gacaacgaca cgttctcacg gttgcagtac gcgcaaagca tgctggctgc gcgcaatgcc
      241 ccgccgccca cccccacacc gtcgccagtg gtgaagccca gtgtttttac gccgctgaac
      301 atcggcatcg cgttagcggt tttgattgtg atcgcgatca tttggcggct cgtgacgagg
      361 ggcagggagg agccgactag ccaccggatt cggtagcttt ttgactccag aaatttcttt
      421 gacacccccg gtcgcgctgc tagcgtttcg gacgcttttt gcgagaacga ggcagtccca
      481 tggtcgttca aattgcggtt tgacgcggct ttgggatttt tagttctacc cgcggggagc
      541 agaccggccc atgtagctca gttggcagag cacgtccttg gtaaggacga ggtcaccggt
      601 tcaatcccgg tcatgggctc cacgacggag ttgcagccgg agtgacagtt aaaacgatag
      661 acgcagttta gttaacaacg acaggagaac aaaatggcta aggaagcatt taaacgcgac
      721 aagccgcacg taaacgtcgg cacgattggt cacgtggacc atggcaagac gacgctcacg
      781 gccgcgatca ccagtgtgct atcgaagaag ggtatggctg aaatacgcgc ctacgattcg
      841 atcgacaatg caccggaaga aaaggagcgc ggcatcacga tcaacaccgc gcacgtggaa
      901 tatcaaagcg acaaacgcca ttacgcgcac gtcgattgcc ccgggcacgc cgattacgtg
      961 aagaacatga tcactggcgc ggcgcaaatg gacggcgcca ttctggtggt ctcggccgcg
     1021 gacggcccga tgccgcagac gcgcgagcac atcctgctcg cccgccaggt tggcgtcccg
     1081 tcgattgtcg tgttcctgaa caaggttgac atggtcgatg accccgaact gctcgacctt
     1141 gttgaaatgg aagtgcgcga tctgctcacg caatacgaat ttccggggga caaaattccg
     1201 atcgtcaaag gcagcgcgct caaagcgctc aatggcgacg ctgaggcgga gaagcaggtg
     1261 ctcgcgcttg tcgaggcgat tgatgattac atccccgttc cggagaggcc gatcgatcaa
     1321 ccgttcctca tgccgatcga agacgtgttc aacatcgaag gccgcggcac cgtcgcgacc
     1381 ggtcgagtcg agcgcggtgt gctcaagaaa atggaggaag tcgagctcgt cggcattcgc
     1441 ccgacgacca aaacagttgc gaccgatatc gaaatgttcc gcaagttgct cgatgaggcg
     1501 cgcgctggtg ataatgtcgg cgtgttgctg cgcggcatca aaaaggaaga tgtggagcgc
     1561 ggccaggtca tcgccaagcc cggctcaatc acgccgcaca agaagttcaa ggcggaagtc
     1621 tacgtgctga gcaaagagga aggcggccgg cacacgccgt tcttcaccaa ctatcggccg
     1681 cagttttact tccgtacaac cgacgtgacc ggcacggtca agctgcccga gggggtagaa
     1741 atggtcatgc ccggcgacaa catcaatatc gaggtggagc tgatcacgcc catcgcaatg
     1801 gagaagacca tccggttcgc cattcgcgaa ggcggcaaaa ccgtcggtgc cggtcgcgta
     1861 accgatattc tggattaaat ttaaccggac gcatacgcca gtagctcaat tggtagagta
     1921 gcggtctcca aaaccgtcgg ttgggggttc gagtccctcc tggcgtgcga taaaaggctg
     1981 caaacgccga acgccaaagg ctgaatttcg aatccagagg gagccaaatg agcgtcgggc
     2041 gttggatgtt ttcttaaaga cttagatgat cacaaggaca aaaaacttct tcaacgaagt
     2101 gaaagttgag ctgcaaaaag cttcctggcc gtgggatccg aaggaaaaag gcttccgcaa
     2161 atacaaggag ctgttcgatt cgaccggcgt cgtcttgatc tcgatgttgt tgcttggcgg
     2221 ctttgtcgcg tttttcgatt tcgtgctcgt gaacttcgtc cacttcttca cccggctgca
     2281 ctgatttatg gcgcaaacac tcgcaggcaa agatcaatgg tacgtcgtgc acgtgctttc
     2341 cggccaggag aataaggtca aggaaaacat cgagaagcgc atcaagactg aagagatggg
     2401 cgatctcatc tatgaggtgc tcgtcccgac ggagcgcgtg tcggagatca agaagggcaa
     2461 gaaaagcgag accacgcgca aattttttcc cggttatctg atcgtcaaca tgcatctgct
     2521 cgatgagaac aacgagctcg tcggacgcac gtggtatttc atccgcgaca cacccggtgt
     2581 gatcggtttt gccggcacaa aggacaaacc aatcccgatg cgccactcgg aggtcgaaag
     2641 catgctcgcg cagatgaaag agcgcgaaga aaaagtaaaa ccaaaagtca gctttgaagt
     2701 cggcgagagc gtaaaggtcg ccgacggccc gttccaaaac cagaccggca ttgtcgaaga
     2761 aatcgatccc gagcgcggca aactccgtgt ctccgtcact atcttcggtc gcgccacgcc
     2821 ggttgaactc gagtactggc aggtcgagcg cggatagagt tttctgacag aattaacaaa
     2881 atgttcagaa tgatttcaga aattttgcag acaacttatt ttgtagattc tgttcattct
     2941 gtctaaatcc aatggccaaa gaagtagtcg ctcacatcaa gttgcagatc cctgccgggc
     3001 aggcgaatcc ggcgccgccc gtcggcaccg cgctcggccc acgcggcgtc aacatcatgg
     3061 ccttttgcaa ggaatttaac gccgctacgc agaaacaagc cggcgacatt cttccggtcg
     3121 tgatcactgt gttcaaagac aagtcgttca cgttcatcac caaaagcccg cccgctgccg
     3181 tcctcttgaa gaaagccgca aacattgccg ccggctctaa agagccgaac aaaaccaagg
     3241 tcggcaaagt gacgcgcaaa caagtcatgg atatcgtcaa caccaagcgc aaagacctga
     3301 acgcgcgcaa cgaccaagct gctttcaaca tcatcgccgg cacggcccgc caaatgggcc
     3361 tcgaaatcgt cgattagtcg cgcggctact ggtggaccgc acaccgcact gcgttcagtg
     3421 gcgttttact tgcggaaatc aagcgcgcct tttttgaggg cgtagacgta accgaccatg
     3481 aggatggaga tgaagctgag catgctccag aagatgaccg cgctgtgggc aatcatgtct
     3541 ttgtagacga ccgcccacgg atacatgaag acgatctcga catcgaaaag gatgaagagc
     3601 atcgcgatga ggtagaattt cacactgaag cgcggctggc cctcgccccc gggtaccatg
     3661 ccgcattcat acggtgtgtc tttggccggg gtgcgcctgc cggtcttgcc gataagcgcg
     3721 ctgacgacta aagcagaagc ggcaaaaacg atcgcgacga ttatctgtag gagaatcggc
     3781 agatattcgc ggagcatcgc ggttcgtccg ctgtcgatct agcggaggaa ttagcgcgtg
     3841 gcggcgggca tgtggccgtt gggcggcgcg atggcgctgg ccagtgcgga cggtaggccc
     3901 ttgtatttct tgccattctt tttcactgca ccgcgcgcca ccagattctg caatttttcg
     3961 taggcgcgca gccggagcat ttcttcgccg ccgctggctg catttcgggc acgaagattt
     4021 tcgtgcacga tgtcaaagag ctctttgaat tcgtaggagg cacgccgaga gagcacggcc
     4081 actagttcct ccgttaccag atcagggatg cggcgggaaa atgcgttttt cttcaggata
     4141 gccatagggc gcgaaacata acacaaaact gctcggctga cagcagaatt ttcgctactg
     4201 gccggcactc tgcgagcgtg ccgaaatggc gcacctcaaa cagggataac cagctgctaa
     4261 tcagcgtgat gtccggcgaa gccccggtag acgacagcca aaccaatcac taaaacggct
     4321 tcgttcgctg tgaccggtcc aatcgattcg gcgatgtagc catcgcgttc gtagcgttgc
     4381 gacgacacga tggggctgag gtgttttgtg attacgtgct gcggcggcaa tttcgcaaga
     4441 tcaacgtcat tcgcgatcgc cggcagaaat gccgcgccca acatgagcag cggccgaact
     4501 gccgcgtcca gccgcgaata caagagcgcc acatcaacgt aggcgaacga ttccgttggc
     4561 tccggcagga tgcgcgctgc tgttttgtat gtcggcgaat tcgacagcgg cgcgttcgac
     4621 ttttggctgc gttctatcgc cgctgtaact gcggacggat cgaggccggc gacaaagagc
     4681 cggtcggaca aaccaagggt ggggctgatc gcgaaaacgg cgcgcgaagc ttcgatcgta
     4741 aaataggtta cgccctcctt ttccgttttg atccaggcgg catcctcatc gagtgccgct
     4801 gtcatggctg ccacgatctt ttgcgcgcgc ggggaatctc tgactgggag agttgcaatg
     4861 aacgatggcc agcgcgcgtt tggcggccaa tcggcgagag cactcaactc caaatcgaac
     4921 gcagctttcc aatcgttcgc tgtgatgccg cttttttgcg ttacgccaat aaacttctgc
     4981 agccagccgc cgagcggagc gatggctgca gcttggttcg tggcgccgag attctgtgga
     5041 ttcagaagcg tcgccaggta gaagaacgtg tcggcggtgc cgagggcctc ggtcgagcgc
     5101 gtcagttttg cgtccgtttg ccgcggcatg gccgcaaaga agacatcgtg catcttgccg
     5161 cgctcaaatc ggatcgctcc gcagatgttg cggatctgct cgatgagcgt gggtttgtcg
     5221 gcggagattt gctgaccgaa tgtggcgcga agcgcgtcga gtttttcggc aaacgccttt
     5281 ggctgcaaat aaacgagcag ggcgtattca ctgggcatgt gaccgaccgc gcctcgatat
     5341 gcctcgtcag attggagcgc ggattgtcga tcttgcttca cggcatccgc tcggtccagc
     5401 agagcttcaa gctcggacac atcgtttgcg gcgaagaacc attgttgtgc gtaagttgtc
     5461 gccaaagtca taccgcctgc agagacagct tcgatctcgt gtcgtccgtg cgtcgttttc
     5521 tcgcgcgtgg cgttgcggtt tcggtgcagc aagttagcgc gccattttcc gatgatttgc
     5581 tccactgcgg cttgccggcc acggaaccgg aaacctgcga gcaaccgcgg cttgttgttc
     5641 tcgatcgagg tgagcgccgc gaaagcgtcc ttcggatcga gctgttcgat atctttgaac
     5701 gcttgcctag cggcatccgc ctttggcgcg gtgtccagcg gtttacgcaa gaaatcctga
     5761 acagccggct cgcgatagag ctggtaaagg tcggattgat gccattgatc ccgcatccga
     5821 gcgaaatccg gggcatgcgc aaaaaaaatc gtttcgcggg gcaatagtgt ggcgaccgac
     5881 cgtgcagccg ccgtagttct ggagatgtaa agccaaccgg cgattgccaa aacggcgagc
     5941 gcgagcgcga acaggaggaa tcgtcgcatg gaaaggcaac tgtaggggcc gctctcctcg
     6001 gcagcaaacg ctcaggcgcg cggatgaaat tgtcgatgga cggctttcag ccgctgttga
     6061 tcgacgtggg tataaacctg ggttgtggag atgtccgcgt ggcccagcat ttcctgaatg
     6121 atgcgcaggt ccgcgccgtt gccgagcaga tgggtggcga aactatgccg gagcagatgt
     6181 ggatagacgt tcgtctcgag tccagagcgc tgcgcaattt gtttcacgat ctgccaaacg
     6241 cgcccggtcg taagtctggt accacgcgca gacagaaaga tttcgctgcc ggttgcacgt
     6301 ttcatcagtt tcggccgctc gagcgacaag tacgccgcga gtgcttctgc tgctttgcga
     6361 cccacgggta cgaggcgcgt cttgttgcct ttgcctatga cgcgcagaat tcgttcttgg
     6421 gcattgaaat tctcgagccg cgccgcggtc aattccgaga tgcgcagacc gctggcgtag
     6481 agcaattcga caatcgcatg gtcgcgcaag ccaagtggca ccttagtatc gatactgtcg
     6541 agtagttgct cgacctgtaa ttcgttgatc gtatcgggca aataacgttc gatccgtggc
     6601 aatgcgagcg cctcggccgg gttgtcagcg atctcaccgc gagcggcgag aaaacggaag
     6661 aagattttga gcgcaacgac gatcaatttg atggacgcgg ccgcgagtcc atttcgtttc
     6721 cgctccgcca gataatcgga caagatagcc aatgtaactt ggcgtgggtt gtcgatcctg
     6781 tgtttcgcct tgcaccattg cgcgaattcg gtgagcgagc gctgggttga gagctggtag
     6841 ttgtttgaga gcccgcgctc gacggcgaga aaccggatga aggcatccag tgcctgctcc
     6901 attgcggaca agatcaactg ttcggccgta ttggcaaata tcgggagttt tccggttgct
     6961 ggggaacatt cgcggcgatt gcgaggttat tgacaagcga aggttaaatg ttcgatgctt
     7021 taacggggat gagcttggat ctgaatagcc tgcttcggga ttggccgcat gagccgggcg
     7081 cgatcaaggt acgcaaaatt gccgggttgg atgggcgcga gaagttgcaa ctgcgcattg
     7141 atcttggggt gctgcaaatg gaaatgagcg ggcgacccga cggtgcgcgg ccgcacggtt
     7201 ttgaatcgct cctggcgtat catcaagacc gtgctgcgcg cgcggaaagc acgggagaag
     7261 aatacgagct cagcgccgag cagtgcaccg agcttcagca ggagggcatc cagtattacc
     7321 atcgttatct cagcttgttt caaatcaacg atttcggcgg cgtggtgcgc gacacgcagc
     7381 ggaatctcga tcttttcgat ttcgtgattg cgcacagtga tcgcgaggag ctggcgtggg
     7441 gtttgcagca gttccggccc tacgtgctga tgatgaatac gcgcgccaag gcgtccatct
     7501 ttctcggcgc ggggaagttt ccggaagcga tccgcgaaat cgagcgcggc cgggacgcga
     7561 tcctcgactt tttccagcgc tcgaattttc cggaactagc ggcgaagagc tcggaaattg
     7621 cgtttttgga ggaatggctc gaggaagtgc gagcgaaacg gccgctctcc aagctcgagc
     7681 tgatgcagcg agagatggaa acagcgatcg caagggagct ctacgagcgg gcagccgaaa
     7741 ttcgggacgc gatcaagctg ctaaagacac agcagtcggt ctagccatgt gagcttattg
     7801 cgaccggctc gttgctggcc ggtttagggc gctaaccctc cgcaaactgt tttgccacga
     7861 cgaaggcgtg cggtgccgga tcaaacctgc agttcgacgc attctgcggt tcgtccaaat
     7921 tggcgacatt cgcgcggaaa aatggccgtt tcgctcatac tagaggcatg tttctagctt
     7981 ttagagccgg ccgtgctgga acgcgctctg tcggacgaag aacataacca gatcctccaa
     8041 acgatcgaga tgtttgaggc catcactcaa acacaacccg acgattatca gtcactggag
     8101 attctgaagg aagcctacca aaaggttggg cggcaggagg attttgtccg cgtctcgcgc
     8161 cggctggccg aatgctattt cagcgccggt gctcacagca acgccatgcg cgaatgcgag
     8221 gcgattctgc tcaacgaacc gaacgcgcca gacatcttgg ccatgctggg cgaaatcgag
     8281 acccgcctgc aaagctccgg cgaagcgatt gtcggcgcac caggcaataa tggcgcgctg
     8341 gacgacactg acggttccct catcgacatt ggcagtcgcg ccggcaaagt cggcaagctc
     8401 gcgaatctgc acgagcgcgg cgacgatcat ctcgcgaaat ttttgatcgt ccagcagctc
     8461 tttgccgagg aagaggtgag ctcggcgctc gagaccgtga cgctgctgaa taaggagctg
     8521 agtggacaag ccttagccgc atcgctgttg gatcagcttt gcaaggcgga caatgcgcgg
     8581 ctggaagcgg tgctctccgc tttgatcgat cggacgaagt tcgcctatgt gccgctcgaa
     8641 tactacgaca tcgaccggca gatcgcgccg atgctgccgg attatctcac ggttggccgg
     8701 ttatttgtcc cgttcgatct ggtcagccgc acggtgatgg tggcgtgctg caatccgttc
     8761 gattccacgg gtcgggaagc attccagcag tcggtcgact acacggtggc gtggtacttg
     8821 gcacggccat ccgcaatcgt caaaaacctg cagtctatct atcgcctcga tgcgcgggtg
     8881 taagcgttca cggtcatgag aagtaaatct ttcgccgaac gtattgccga cgtgctcatt
     8941 gaggacgggc tgctgctccc caatcagctt gaggaagccg tcaacattca aaaaactgaa
     9001 ggcggccggt tgctgaaaat cctgaccgat cgacaatttg tcaccgagca agacatggcc
     9061 ttcagcatgg gccggtgtct gaacacgcct ccggtcaatc tgagcaagct tcgcgtgccc
     9121 gaagaagtga tgtcgctcgt gccgcgagaa atggccaagg caaacaagct ggttccgatc
     9181 gcgcgactcg atggcaagct gttcgtggcc atggccgacc cgacgaacgt gctcgcagtc
     9241 gatgatctga aacggcgggt gcagctcgag atcgtgccga tgatcgcgac cgagcaggcg
     9301 gtgaatgacg tgctgagcgg catccacact tccagcgcca acatgagcca ggtgctgaaa
     9361 aaggtcgcag aagaagctgc gcagaccagc gaagtcgaag tccatgcggc caagcacgag
     9421 gagatcgatc tcgaccggtt ggcggccgac agcgaggacg cgccggtcat caaaatcgta
     9481 aacctggtcc tcgtccaggc tatgaaggaa aaggcgtccg acattcacat cgagccgttt
     9541 cagaattcgc tcaagctgcg ttaccgcatc gacggcgaat tggtcctcgc cgagtcgccg
     9601 ccaaaagcgc tccaactcgc gatcacctcg cgcattaaaa ttctcgccgg cctgaatatt
     9661 gccgagcgcc gtctgccgca ggacggtcgc ttccggatca gggtgatggg gaaggaagtc
     9721 gatctgcgaa tctcgatgtt gccgaccgcg cacggcgaaa aaattgtgat ccgcattttg
     9781 gacaaagcgt cgctcacggg gggcatcgac cagatgggca tcgacgccga cacgctgtcg
     9841 aaattccgca aagcgatcga cgcaccgcac gggatgattc tcgtaactgg ccccaccggc
     9901 tcgggtaaaa cgacgaccct ttattcagtc ctgcacgagc tgaacaatcc ggcttacaac
     9961 atcgtgacgg ttgaagaccc gattgagtac gagctgatgg gcatcaacca agtcgccgtg
    10021 cgcagcgata tcggcctgga tttctcgtcc gcactgcggt cgatcctccg ccaagacccc
    10081 gacatcgtga tggtcggcga aattcgcgac aacgaaacgg ccgacatcgc ggtgaaagcc
    10141 gcccttaccg gtcaccaggt tttgagcact ttgcatacga acgacgccgc gggcgccatc
    10201 acccgcctcg atgacatggg tatcgagccc ttcctcattt ccagctccat cctcatggcc
    10261 tgcgcgcaac gtttggtccg gcgcatctgc acgaactgcc gcgaagaatt tgtcccggag
    10321 ctggagattt ttgagcgact cggaatagag caaaccaagg ggaccgtgtt ctatcacggc
    10381 accggctgcg accgctgcaa agggcgcggt tatcttggac gcgtggcgat tgttgaagcg
    10441 cttccggtga gcgaagcggt tcgccggttg atcatcaaac gcgcctccgc cgcagtcatt
    10501 aaaaaccagg cgatgagcga agggatgaag acgctgcgca tggtgggcgt ggagaaagcg
    10561 gtcgagggtg tgacaacgct ggaggagatc tggcgtgtca cttccgagga tcattaggat
    10621 gaaaattcca ttctctggtc tatacgaaaa actgcgctct cgcgtctcag cgccatccgc
    10681 tgcgtcgcag cggccggtcg tcagtcaacc ggtggtgaaa agtggaacga gcttgagcaa
    10741 aacggttctg ccgaacacga cgcgcaaagt agctgaacct gacccgctgc aaaccgcggc
    10801 aggagtttta gccaaagcag cgtcacaatc agccgtccgc atcgaggcgg tgccgtttac
    10861 tgcgtcgaca aagccgcggg aaacctcaga acgctccgcg gtaaagcttg aggcgaaaac
    10921 cgagcgcgcg atttcattat gcctggacga catcctcgac cggctgccgc cgggcgtcgt
    10981 gaaagcgcgc gagagtttcg atccaaagcg ccacattttg ctcaaggcgt cagaactgga
    11041 aaaggggatg gcaagtggca aacctgcggt cgcgctgacc aatatttacc agcaagtgcc
    11101 agaggtgttt ttgcacgccc ccgcggcgac tgactccacc acggtgtatc tgccgttcga
    11161 gaaagtgctc gcgcagttca tgagcttaca ggtgcgatcg gatcaggagc acgagcaggt
    11221 cgttccgcac gtggaaacgc cgatcctgca agtcacgctc gaagataagg accgcttcgg
    11281 cacaccgatc gagccgctgc aaagttcgtc ggcgccctcg gcgcgcgccg cgtctcctac
    11341 taccagtagc atccctgctg cggaagcaaa aacgacagca gttgcaactc cgaaaccgcc
    11401 ccccattcgg gcgccaattc ctctagtcgc caaactgccg ctcgctcctg ccactcggtc
    11461 gcagccaggc gaagcggcgg cgccttcagt agagcttcca ccgaacggga cgggtgtgcc
    11521 cgcctctgag agagttccag cctcaagcgg gccgcccgcc ccaattacgc caagctcgcc
    11581 accaccaccg ccgattccgt tcaaagttcc gccgccagcg agtgacattc ggccaagatt
    11641 catccgcgtg ccgggagtcg agcccagcga gggagccagc tccacgcaaa agctaacgcc
    11701 ttcgcgatcg cctgacccga ccatcgcctt gacgctgtta cccattcttg aagaattgcc
    11761 gccttttcaa ttgagtggta gtccgtctgt cgtcccggac gatgtgcgca tccgcttgcc
    11821 actttcgctc attgagcctc aactggcgac cggctgcgta acagttccgg caaaggtgtt
    11881 gcaaaaggcg atgcctgcgg agtaccgcag cctcatcaaa agcgatacgg cggagacgcc
    11941 gatttcgttg tcgctatccg aggtattgaa gcatgtgcct gtggctcttc tgcgcagacg
    12001 tgaggatcag gagcaaccgc cggccgtgga aaacgtcgag acgccgatct caatcgctgc
    12061 cgacgaagat gccaaacgtt tagctgaatc gaggcagacc cgctctgata aacgggccga
    12121 gggagtgccg attaagttcg agagggatac agtcactgcg gaggcgaaaa acacggaagc
    12181 tgcagaggag cgcctcgagc agccggagca gccactcgac gcgcggactg ttgtggcacg
    12241 ggcaaacgct ttgccgggcg tggcggtatg cgcagtcact tttgccgacg gcctgaatct
    12301 tgccggcaat ctgcccgccg agctcggcgc ggacggactc tgcgccttgg ccgcttcgct
    12361 cctgcaaaag atcgacaatc acctgcacga gcctaaaatc ggcccgctcg tgggcatgac
    12421 cctgcacgcg gcgaaatcga ttacgtttct ccggcgcgac aacatcgtgc tcgccgcgtt
    12481 gcatagcgga gacgatttgc cgactggaac gcgcgagaag ctcgcaacgc tcgttgagaa
    12541 gctttcccgc acttacgccg aatcggaacc tgcccatgtc catcattaat tacgccagcc
    12601 gcgagataca gtttaaggtt gtctattacg gcccggcgct ctgcggaaaa acgactaatc
    12661 ttaattacat ccatcagcgt atcactccgc aaaaccgcgg cgatctcatc tcgctcgcca
    12721 ctgctgccga tcgcaccctg ttcttcgatt ttcttccgct caacgccgtg gtcatcaaag
    12781 gcttcgtcac caagttccag ctctataccg tgccgggcca ggtgatctac aacgccaccc
    12841 gtcagctcgt tctgcgcagc gttgatggcg tcgttttcgt tgccgattcg cagtgggaaa
    12901 agatggagga gaacgtcgag agctttaaaa acctggccga taatctggcg cagcagaatg
    12961 tgtcgatcga cgaagtgccc tgcgtgctcc agtacaacaa gcgcgatctg ccgaatatcg
    13021 cgccgatcaa ttacatggag tacgtgctga acaacggcaa gaagcggttg cagagcttcg
    13081 aagtgatttc cagcactggc cagaacgtct tcgccagcct caatgctgtt tcgcagactc
    13141 tgctgcacaa attcagcaag caggatgacg cgccgcagcg tgcgccggcg cccgtggcaa
    13201 cccccatcga atcaccgctc ggccggccac acagcgcgcc ggtatgaacg cgattccggt
    13261 cctgacgatt gaagatgtcg ccgcggtcga cggcgtgctc gaagattttt tgcgtaaaac
    13321 cgaagcggac ctgacggtgg tcatcgatcg cggcggtaac gtcatctcgc aatttggcga
    13381 tatggacgtc accgatgtga ccgtagttgc cgccctcgcc gcaggctcat tcgcagcaac
    13441 acgcgaactg gcacgcagaa tcggcgagct cgagtttaac gcgctctatc atcaaggcaa
    13501 cggcacgcac atcttcatga actcggtcga cgaggacaca atcatgacga ccgtgttcgg
    13561 ccgaggcacc accgtagggc tcgtcaggtt ctattcggcg gcggccgcgc aacggctcgc
    13621 cgagatcctc aaatcgctcc ggcacaaatc gccgacgctc aagctggcgc ccgtctcgag
    13681 cgcgaccgtg ttcgctgatc gttaggatcc accgtttcgc taacggtgcg gcggcaggcc
    13741 gtacggtcga caatcagtcg tccacgctgc gctatgactc gaaggcggtt gtcacaagcg
    13801 cggcgttgtg ccccccgaag ccgaacgaat tgttcaacgc gactcggacc gttcgttcct
    13861 gcgccacatt gggcgtgtaa ttgagatcgc actgttcgtc gggattttcg aggttgatcg
    13921 tcggtggaat aactgaatcg cggatggcca acgcgcacac gaccgtttca accgcgcccg
    13981 ccccgcctaa caaatggccg gtcatcgatt tggtggcact gatcgatacg tcgtaagcct
    14041 tcttgccaaa gactttctta atggcttgcg tttcgctgat gtcgccgagc cctgtcgagg
    14101 tggcatgggc attgatgtag tcaacctcat cgggtgaaac gccggcgtgc tccagcgcca
    14161 gttgcatcgc acgcgccgcg ccctcgcctt cgggcggcgg tgcggtcatg tggtaggcgt
    14221 cagccgatac gccgtagccg gacagctcgc agtagatttt cgcgccgcgc gcccgggcgt
    14281 gttcgagctc ttccaccacg accacacccg cgccctcgcc caagacaaaa ccgtcgcggt
    14341 cacggtcgaa cggtcgcgag gcgcgttcgg gttcgtcatt gcgcagactg agcgctttca
    14401 ttgccgaaaa tccggcgagc ccgatgggaa ctatagaggc ctcagagccg ccggccagga
    14461 agatgtcggc atcgccaaac ttgatcgtgc gccacgactc tccgatcgcg ttgttcgacg
    14521 tggcacaggc ggtgacgatg cagaggttcg gcccctgcag tgcgtactcc atcgagataa
    14581 ggccgctggc catgttgctg atcaacattg gaatcgtgaa tggcgacgtc cgtgacggac
    14641 ctttggtgag caggattcgg tattgatctt ctaacgtttt caggccgcca atgccgctgc
    14701 tcacgatcac gccaaagcgc tcgcgattcg ttctttctag atcgatcccg gcatcctgca
    14761 gcgccatttt cgccgccgcc agggcgagct gcgcgaagcg gtccgtacgc cgcacgtctt
    14821 tggcattttt gaaataccgt tttggttcaa agccgcggac ttcccctcct attttgcaat
    14881 cgtgctcact cgtgtcgaaa gcttcgatat ggcgaatccc gctgacgccg ttcttcaggt
    14941 ttgaccagaa ggtctcgacg tcgttgccga gcggagtgac cgcacccaaa cccgtaatga
    15001 cgactctgcg atcggtattc atcaagtgac gagtgattag tgacgggtga cgagaaaaga
    15061 gcgcgcggtg acaaatagct cgtcactcat cactcgtcac ctgtcacctc actttgtgcc
    15121 catcccgaca ctttgcacgg cttgaaaaag tttgccttcg gtgcggatcg atggtgcgat
    15181 gatgatctcc gtctgctgaa attccgacaa atcgcgcgcg cccacgttcc ccatgcaggt
    15241 cgtgattgcg ccgacgagat tctggctgcc gtcgtccact tccgccgggc cgaacaaaat
    15301 ttgcttcaga ggcccagttg ttcccacttt aatgcgcgtg ccgcgtggaa ggttcacatg
    15361 cggcgtcgcc atcccccaat ggtagccgca gccgggcgcc tcctgcgctt tggcgaaggc
    15421 gcttccgacc atcacggcgt ccgcgccgct ggcgagcgct ttgcacacgt cgcccccttt
    15481 gctcatcccc ccgtcggtga tgatcggcac gtaacgcgcc gtttttttga aataggcatc
    15541 gcgggctgcg gcgcaatcca ccgtggcggt gacttgcggc acgcccagcc caaggacacc
    15601 ccgcgacgtg cacgccgctc ccggaccaac accgaccaag accgcagccg gaccacactc
    15661 catgatgtcc agcgtcacgt catagccaac cgtgttgccg acgatcaccg gtatgcgcat
    15721 ctcggcgcaa aattttggca gatccagcga tttatattcc gaggaaatgt gccgcaccgt
    15781 cgaaactgtg ctttgcacga cgaaaacatc cgcgcccgct tcctgtgcaa ttttcccgaa
    15841 ttgctcggcg cgctgcggaa tgcagctcac cgccgcgagt gcgccgccgt ctttgatctg
    15901 gcggatgcgt gcggcaatca gctcttcctg caccggctcc tggtaaattt tctgcaataa
    15961 gccggtgatc tgcgacctgt tcgcttcgac gattttttgc aggacctctt gcggattttt
    16021 atagcgcgtt tgcactcctt cgaggttgag cacggctaag ccgccgagct tgcccatggc
    16081 aatggcaaag cgaacatcga caactccatc catcgcgctt gccaggatcg ggattttgag
    16141 ctcgagcggt gcaccgtcct tgcgcggcag gcgaaacgtg atgtcgacct cgtttggatt
    16201 gattgtcacc cggcccggca caagcgcaat ttcatcgaag ccgtaggtca cccgcgcttt
    16261 acggttacgg ccgatccaca ttcccatttg ctagatgcgc tccagtttaa atcgttcgtt
    16321 taagctcccg cagcgcgggc accgcggaag caacgtgtcg gtacggtctt cgaactcgaa
    16381 cgcgcacatc gcgcaacgca cgcgatgctg taaagctcgg cgttcgcgtc gcccgcgctt
    16441 ccactctccc actatccaaa ggagaaaaac cgccgcaaga aaaagcagca ggtaaatcga
    16501 gaccagcgcg gcgaggctga cgcggatcac tgtgcgctgg tcgcggctcc tgagtttgcc
    16561 cgcggtcggt cgatgtccgt tccccactca acagtcgcga ggccccacgt gacgctgcct
    16621 tcgtctgtct tgccggctgt cgatcttacg gggaatcggc agagcacgag atgctgccgc
    16681 gcctgctcgt cgagggctgc atcgccggat gagtttatcg taaaacagtg ctgaatctca
    16741 ccgcgcgcgt tcactgccac ccgaaattgc acggcctgcg gcggttcatg tgtagtcacg
    16801 gtaaactttg tgtgggaaca cgttgcagca ccgattgcgg ccagttcgtc cgaaaacaaa
    16861 accgtcgtgg aaaattcgcg acctcgcggc ggaggtttgg gatggataat ggagaccgga
    16921 ccgggtggct gtgcactcgg gacgtgtaag tccattatta gtggcggcag ttgttgaatc
    16981 gccggctcgt tcgccaagta ggaaggcacg tgctccacct tcggcagcgc gcgtagacgc
    17041 gattctggcg ggcgttgcgt ggcaaaggcg agcgcaggat cttccgcctc aatccggtac
    17101 agcagattgc ggccctcttc cgagctcggc gaaatcacac tcacccgcgc cggcggaggc
    17161 aagattgcaa tcgtcggtgg gtaaacaatc tgaaacagat aaaaacaaaa tgcgtggccg
    17221 aggaatgatg ctgtgacaaa accgagcatc gtgaaatcac ggcggtgcgg cgggtcccaa
    17281 tcgaagatca agcgtgggaa ttcgtcggca gcggcagcgt tcacggtggc ggagctgtcg
    17341 ccaaggcgac agaagcgatt ccgtgctcca gcgcggcgtt cgccacttca gcgacggttt
    17401 cgtacggcgt cagccgatcg gcccggatca cgagtgatgt gccgtcgcgc ttggccgcat
    17461 cgaacaatgg tccaagttgc tcaacggtta ctttttgatc gcgaaagtaa attgccgcta
    17521 cagcgcctcc cgtgatgctc acgatctgct ggtttggctg cggcccgagc gtgaagcgtg
    17581 agaacggcag tgatacggca atcccaggtt gtaaaaggaa gttcgaccct agcaacaaaa
    17641 agaacacaat aatgaaaagc acgtccagca acggaaatac aagcagccag ccgaagttgt
    17701 attcttttgt gcgggtgagc ttcattgcac ggttgtcggt tctcggccta acgaattgtt
    17761 gcccaccacg aatccgtcga gcaacaagtt gatgtcaacg atccagccgc acctcggctt
    17821 cgcttctctg ctccggctgc tcaaagctga tgatcccgcc aatcggacgc gtttcagtca
    17881 gcagatgcat gatctcaatg ccggcacgtt ccatttcgtg cagcatcgtg ttgacgcgtg
    17941 aggaaagata gctgtaggca acaaacgtgg gcgtcgcgac caccagaccc gctgccgtcg
    18001 tgagcaagct cttgtaaatc ccgctcgaaa gctcagtcac ggtggcgtag ccaccgtggg
    18061 acgaaattgt gccgaaggca tcgatcattc cagtcactgt gccgagcaaa cccagcagcg
    18121 gcgccaaaaa agcgagcgtg gcgagcacgc cgagatagcg ttcgagcttc ggcacttcga
    18181 gttgcgcggc ttcctgcacg atctcccgca actctgagcg cggcgcgtca tggcggatga
    18241 tcgcggcgtg aatcacccgc gcgattggcc ccggtgtacc ggccgattcg tgtagcgcct
    18301 cggcgaaatt gcggcgctgg atcaaattcg taagtccctt caagaactcg ccgacattga
    18361 tgctggcacg gtgaaagtag aacagccgct ctgcgaatac cgcgattgag attatgctgc
    18421 agaccagaat cggccacatc agcaggccgc ccttctggat gtactcaatc atcggattcg
    18481 ttgatgtaga cttttttacc cgccgtggca aggagctcgc gtttagcgtc cgtttttgag
    18541 acgcggcttc actgaagtcg gcgtcgctgg cgcgagcggg gccacacgac tgattagttt
    18601 accgtcggca tcgaaaaccg agtagcccgt cggctttccg cccccatcat agttgtaaac
    18661 aattttgtgc aacacagcgt cgccctcact gaattgcgtt tcctcctcaa gttttcctgc
    18721 gctgtcgtac ttgtagcggc tcttgaaacg cagctttcca tccggggcga agaccagcgc
    18781 gctcgcgaag cgcccgctat cgtccaactg gtagtgaatc ttctcccgca gtttgccatc
    18841 cgcatctgtc gctgtcgccg tcgccgtatg cttggcgtcg ttgaatttgt aaactgtgcg
    18901 cgagccatcc tgattaactg acatggtcag gcgaacgggg gtattgctgt cctcgccggc
    18961 ctggccgaaa ataattgtcc gcgcggcgag aaacgccgcg catgcgattg tcgatcttgc
    19021 aaagggcgac atgggcgagg ggaagagata gtaaaacgcg ggcaggcagc tgcaaacagg
    19081 gggcattaat ttttattgac gcttggcacg cgaggtggtt taaagtgggg gaaagtgggt
    19141 caggatggat cgtaactcca ttcccgcatg ccgtcacctt cgccaactca atctttctac
    19201 gccggtgaat ttcgtcactt catcgacgag aaaaatcgca tcaccattcc atcacgctgg
    19261 cgccgccgtg agggtgaaga gttcattctt ctccccgaag cgagtcatca attcctgctc
    19321 gttatgtcac cggacgagtt cgcgcgcatg agctcggctg cggaaaatac atcgaacgtt
    19381 tccgcgcgcg atcgccgcgt tttcttgcgg caattgcatt cgcgcgcgca acacgccgcg
    19441 gccgaccgac agggtcggct cgttttgccg gaagaacttt gcaagcaaac cggcctcaaa
    19501 ggcgaggtcg ctctcgttgg tggccgcggc cggtttgaga tttggaattt gcagcgctgg
    19561 aaacgcgcac acgaggagga aacgcccacg tatcagcacg tcgccggtgt gatcggcctc
    19621 taaacaatgc caccgtccaa actagaacta aatcaaatat gaatgcattg ctctttgaac
    19681 gaccagtctg gtttgcgacc ggtccgctcg tgctggaaga ggaggcgcag gacatggagg
    19741 attttaccta tcaccgtccg gtactagccc gcgaagttat cgagttgctc gcgccgaaac
    19801 ccggctcact cgtgatcgac gccacgtgcg gcggcggggg acacaccgaa gcgatcctcg
    19861 ccgctggcgc ggatgtgctt gcactcgacc aggatcccga cgcgatcgag tacgcgcaga
    19921 aggaactcgc tcgctttggc ggtcgcgtca cactgcacca ggccaacttc cgtcacgccg
    19981 acaaaattct cgacgagctt ggaattgcga aagtcggcgg cgcacttgtc gatcttggcg
    20041 tgtcgtcgcg ccagctcgaa aacgccgagc gcgggttcag catgatgcgc aatggccccc
    20101 tcgacatgcg catggacccg cgcacggagc taaccgccgc aacgatcgtc aacaactaca
    20161 gtgaagaaga gctcacccga ttgtttcgcg agctcggtga agaaccggcc gcgcgtcgca
    20221 ttgccagtat tatcgtcaaa gaacgcaaaa ccaccccatt tcgggaaacg cttcagctcg
    20281 cgcgggcgat cgaaaaagtc gtgggccgcc atggccgccg acatcccgcc acgcaggtgt
    20341 ttcaagcatt gcggatgcag gtgaacgacg agctcggcgc gcttcctgac ggtttgcgcg
    20401 tgatgaccga gcggcttgag ccgggcgcac gcatcgcggt cattgctttc cattcgctcg
    20461 aggatcgaat cgtcaaaaac ttcttccgcg accacagccg cgactggctc gaccggcccg
    20521 aatggcccga gccacggcgc aatccggagt acgatctcaa aataatcacg cccaagccga
    20581 ttgaacccgg cgaggaagaa caacgcgcca atccccgctc gcgcagtgcg aagttgcgcg
    20641 ttgcggaaaa acttgcatga accgctcgcg gcgaaactgg aacacggtcg atgcggcatc
    20701 gttggttcgc tggcttgtac tcacagcctt tctggcgctg accggcctca gctacgtgta
    20761 tctgacgttg caactttacc atctcggcga ccgcaagaag gcgctcgaaa acgaactcgt
    20821 cagtttgcgc agccagaatg atgtggtcag tgcacagatt gccgcgttga catcacgcat
    20881 cgcgctgcaa agacggctca aggaaggtta cttgaagatg gtgccaatta ctgagcgcaa
    20941 tattgtgcgc ctgacggtcc cggcgcgttc tgccgatgaa gatcgtgtcc agccggtcgc
    21001 aaaccaaacg gtgcaccgat gaaatggaat agccgcgtcc gctgcgcact cgtcggcctg
    21061 gcgttcactg cgctgttcag cgctttttca tttcgcttgg tctatctgca aatcatcaaa
    21121 cacgaggagt acgccggtct cgccgccgag aagcacggcg acaaacagat catccgcgca
    21181 gagcgtggcg agattttcga cgtgaacaat gaagtgctcg cgcacaacgt gccggtggaa
    21241 acggtcgtcg ccgacggcac gcacctgaat gatcgcgatg ccactgtcga tcttgtcagc
    21301 gccgaattga acctgcctcg gcacgaagtc cgggaaaaac tggcgggcga tcgccgttac
    21361 gtcgtgatca agcgggatgt gccaagagcc gtggcggagt cactacgcag ccaactgcgg
    21421 gcgcggaatc gccgcggcat ctacttcgaa catgacgcca gccgcattta tccgaacggc
    21481 tcgatgctct gtcacgctat cggttttact gatttcgaac atcgcggcat tcaaggcgtg
    21541 gaagcttcga tggaggaata cttgcgtggc caggacggct accgcttcat cgagcacaat
    21601 tgcgccggcc aggagattgt tctctatcgc gggcaggaac gtgcgccgcg caacggctac
    21661 gacgttcatc tcacggtcga tcttcatctg caggacattg tcgaaaacga aatcgacgcc
    21721 gcagtgcgcg aataccaccc ggccaaggcc acgatcatcc tgatgcgccc gcgaaccggc
    21781 gagattcttg cgatggcaaa tcgcccgaac ttcgatctca accagcgctc ccaagccgcg
    21841 ccggagcaga tgaaaaaccg cgccataatc gatctgatgg agccgggctc caccttcaag
    21901 atcgtcgccg cagctgcggc gctgaatgag cgcacagtca atcccgacac agagattttc
    21961 tgcgagcacg gcctctggaa cttcggcggc agcgcactgc acgatcatcg gagtttcgcc
    22021 gatttgagtg tcaaagacgt tctgatcaaa tcgagcaaca tcggcgcggc gaagcttgcc
    22081 ctcggcgtcg gcgagcagaa attctacgaa tacattcgcc gctttggttt tggcgaacgc
    22141 accggcatcg agctgcccgg tgagatcagc ggcatgattc gttcgccgca atcgtggagt
    22201 aaaatttcga tcacgcgcat cccgatgggt cacgagatcg gcgtcactcc actgcagatg
    22261 acggccgcga tggcaacaat tgctaacgat ggaaaactcg tcgccccgcg cattgtgaag
    22321 tcgatcacca ctccagacgg caaaaccatc agctcgtttt caccaacggt ggtgcggcag
    22381 gtaatttctc cccagacggc cagacagata ggtgatgcgc tgcgcggtgt cgtgagcgat
    22441 cgcggcaccg ccgcagcggc cgccattccc ggattcacca tcgccggcaa aaccggcacg
    22501 gcgcaaaagg tcgatccgaa gggcggctac gaaactggta aatacgttgt ctccttcagc
    22561 ggctaccttc cggccgaaca tccggagttt gtcggcttcg tcgtgttgga tgacgcgcac
    22621 acgagcaagc cggagctaaa ctacggcggc cttgtcgccg gcccgatctt tgcgcgaatc
    22681 gccgagaaag cggcgcgcta tctcgacctg caacccaggg aggaaattcg cagggcgctt
    22741 ccagtggaga cggtcgctat cagcaaggcg gtggcgcgtt gagttcgaat ggtttcgcga
    22801 gttgaacgcc tggcgcggac atgttgaacg cgcatgcagc tcgagaaact tctccgcgcc
    22861 accgccgtcc gccaggtcat cgggccgcct gatcgcgacg tggaaagcgt cgcgtatgac
    22921 tcgcggcgag tgcaaaccaa ttcgatgttc gtcgccctcc gcggcgagaa aacggacggc
    22981 caccaattca tcggccaagc catcgaccac ggcgcgtcgg tgatcgtcgc tgagcgcgaa
    23041 gaaaaacatc cgcgcgccac ctgcatcgtt gtcgagaaca cgcgcatcgc gttggccgac
    23101 atcgcggtcg cgttttatcg gcggccggcc gagcgactga agctcgccgg gattaccggg
    23161 accaatggca agaccacgac cacgtttctc ctcaaacaca tttgcgaaga ggcgggactg
    23221 cgctgcggtt taatcggcac agtccgctat gaagttgccg aacgcatttt gccggcgact
    23281 cgcaccacac ccgagtcact cgatttgcag gaactgctcg ctgcaatcgt caatgccggt
    23341 tgtcgcgccg ccacgatgga agtttcgtcg cacgcgctgg cgcaggaacg cacgcgcggc
    23401 atcgaatggg acgtggccgt tttcacgaac ctctcccagg accatctcga ttttcacggc
    23461 acgatggaaa gctatttcga agcgaaagcg cggcttttca cggagctgtc ggcgcagcag
    23521 aagaagcgcg aacctgtcgc ggtcgtgaac atcgacgatc cttatggcga acagttgctc
    23581 cgcaagatcg acaataccgt ttcggtcatc acttacggca gcggggttcg cgcgaatttt
    23641 cgcgcttcga gttaccgcgc gaggtttggc agcacgtcat atcaactcga agcgcgcggc
    23701 cgcagttatc ttgttcggct gccgctcatt ggtcggttca atgtcgccaa ttcgatggcc
    23761 gcgctcgcag ccgcaaccgc gctcgggatc aatttgcgtc aggcggttct gagcctggcc
    23821 aaatcgccgc aggtgccggg ccggctggaa atggttccgg cgaaacgaaa gttccaggtg
    23881 tttgtcgatt atgcgcacac gcctgacgct cttctcaacg tgatcaaaac tttgcgcgag
    23941 ctcgagccgc ggcgcttgat tgtcgttttc ggttgcggtg gaaaccgcga ccggcaaaag
    24001 cgtccgctca tggccgaagt cgtcgatcgc aatgcggatt atgcgatcat cacctccgat
    24061 aatccgcgca aagaagatcc ggcggccatc ctctccgaag tcgaaaaagg ttttcgatcc
    24121 aatcacttcg agaaaattgt cgaccgcacg caagcgatca atcgcgctgt caagctcgtc
    24181 cagccgcgcg acattgtgtt gatcgccggt aagggccacg aaacctatca ggagttcgcc
    24241 gatcggacgg ttccattcga tgatattcaa gtcgcccgcc gcgcgatcga aaaccatccg
    24301 gtggaatttc aatgaatcga ttgtcgatct tgcaaattgc cgaattcgcg gacggcaagc
    24361 tctcgggcga cgccggtagc actctcgtcg agaaaatcag taccgattcg cgcactttga
    24421 aacccggcga attgttcgtc gcgctgcacg gcgaaaaatt cgacggccac aaattcgtcg
    24481 aggcggcggc gaacgccggc gccgccggtg ctattgtcga tgttgcgtgg accggaaatg
    24541 ctccggctaa gttcccgctg attcgcgtcc aagatacgct gcaggcgtac cagaatctcg
    24601 ccgcaaaata tcgcggctcg ctctcgctgc gcgtgatcgc cattaccggc agcaacggca
    24661 aaacgagcac gaaggatttc accgccgcgg tgctcgcgcg aaaatttcgc gtcacgaaaa
    24721 cggaagggaa ttttaacaat cacgtcggtc tgccgcgcac gatcttacag gctacgtccc
    24781 aggacgaagt cgccgtttgg gaaattggga tgaatcatcc cggtgaaatc gccgccttgg
    24841 cgaaactggc tgcgcccgac gtggcgatta ttacgaacat tggcctcgcg cacatcgagt
    24901 tcatgggttc gtgcgaagca atcgcgcggg aaaaaggcgc gctcgccgaa gcagtaggcg
    24961 cgagtggctc cgtgatcttg aatgcggatg atccgttcag caaaagcatc gctcaacgca
    25021 caaacgccaa cgtaattttc gccggcacga gtggtgggac gattcgcgcg aatgacatca
    25081 cgcagagcgc tgatggttcg gaattcacca tttctgaagg cgcgcaccgc tgccgcgcgc
    25141 a
//
LOCUS       DASTDG010000148         6840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_878808, whole genome shotgun sequence.
ACCESSION   DASTDG010000148 DASTDG010000000
VERSION     DASTDG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878808"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFO30_04825"
     CDS             complement(<1..1124)
                     /locus_tag="VFO30_04825"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HET9418643.1"
                     /translation="MTTEPMAERYAQMRDVARVRERALFPLQRVPPELELQARWFAGD
                     FGRHFVATTGDKIDIVQFGIWNREAGPDFSDAAIRVNGSEPTRGSIEFDLTDINWETH
                     GHAANAAFENTVLHVFVNESAQKFFTRTLSNREVLQVRVDLNVLPETFSANIPLARPG
                     RCQAPLKDLPEEQVRSVLDAAAQFRLQRKAARLRNKIDNHGRDEALFQEIASALGYKQ
                     NKLPFTLVAQRLPLKLLRENMGDCEALLFGLAGFLESPDLDVYARSTKEYVRQLWDKW
                     WPHRNALQRLKLSPETWRLSSTRPLNHPQRRVAALAMLACEWPGLSRSLGKRSATATA
                     EFFAGLDHPFWNFHYTLTAGPASKHMALIGESRVADILANV"
     gene            1260..2522
                     /gene="serS"
                     /locus_tag="VFO30_04830"
     CDS             1260..2522
                     /gene="serS"
                     /locus_tag="VFO30_04830"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9418644.1"
                     /translation="MLDIRLVREKPDVVRERLATRGGGDEAKIDNLLQIDVERRKAQT
                     ALQQLNAERNRLSKEVGAKKSRGEATGEIETQVRKIGEQITDLSQRTSTLDEEEKNLL
                     LQIPNLPHATVPFGKDPSANRLVRSWGEKPKLEQALDHVALAEKLKLLDLARAAKLSG
                     SGFICFTGAGARLERALINFMLDLHTREHGYVEISPPFVVRRDCMIGTTQLPKFEPDM
                     YGLENGELFLVPTAEVPVTNFHREEVIGDLPKRFVAYTPCFRREAGSAGRETRGIIRV
                     HQFDKVELVKITPVEKSYPELETLLTDAERVLQLLELHYRVVELCTGDLVFGSAKTYD
                     IEVWSPGQDNYLEVSSCSNYEDFQARRMQLRYKGGDGKNTFCHTLNGSGLALPRLFAA
                     LIEHNQQPDGSIRIPDKLQPYFGASEIR"
     gene            complement(2581..3456)
                     /locus_tag="VFO30_04835"
     CDS             complement(2581..3456)
                     /locus_tag="VFO30_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9418645.1"
                     /translation="MNLTGQQKIRIGTCAWSFAEWRESFYPAELPESQWLEFYARHFS
                     AVEIDSTFYHPPAEDTVLRWVEATPANFRFACKLPRNITHVCRLRDCSAELNGFLRAI
                     EPLTPKLQAILIQLPPSFTPKDGKPALRNFLKQLPRDLRFAIEFRHPGWHRPQIIGLL
                     EKFRICWVWADTSQLNERNLAPFEFLPRTTDFLYLRLLGDYATKYDKDGLFIHRYSKL
                     LWKREAALESWSLKIERHLPEVRSVCVFASNHFEGFAPETAQRLAQRLGFELPLPAEV
                     EKIAAEQERSQLELL"
     gene            3584..4864
                     /gene="bamD"
                     /locus_tag="VFO30_04840"
     CDS             3584..4864
                     /gene="bamD"
                     /locus_tag="VFO30_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9418646.1"
                     /translation="MDLPARSLVFLIGFVGLSIPQLGTAAVVFKPGENAKLVPPGILA
                     PGEEEMNGNAQELFQKGQAAERAGNLGSAIRAYRALVRRYPRDALAAGALFKMAELQE
                     QSHQYLKAAVTYRALVERYPSSDRFNEAIEAQFRIGEMYLAGKKQRLLGIPIGSALER
                     AVEIFASVVRTAPYGKYTARAQFDIGLVREKQGAKDAALEAYQAVADKFPNEPVAADA
                     QYQVGYIWFTAARQGARDIAATHNAKTAFEDFLFRHPNSEKAAQARANLQLLEHKQTN
                     SSYEVAKFYDKQKKYRAAAIYYNEVIRQQPGSAESSQAQKRIDQLRAKYGDAALQPRA
                     GDIGAEQKKTSERDSGVAKNASGEPPGASSVKSNAPLPPSDADTALPPPASLLPDTTT
                     APATLDTDSTTTSSPSRGASPAPASPDASASPSP"
     gene            4861..5364
                     /locus_tag="VFO30_04845"
     CDS             4861..5364
                     /locus_tag="VFO30_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET9418647.1"
                     /translation="MKSLIAVALTASCLSGCLGYHIGPVKPYYLRDIHSIAVPTFTNR
                     TLLPRVEVLVTDTVIKQLQQDGTFEVAGEDKADAVLKGEITHVERAPARPVRGNVLAT
                     AEFALTVYLKYSVVNKSGSQLAVNETYGSTTFFVGTDVITDERQALPLATAQLATNLV
                     AQLTEGW"
     gene            5371..6252
                     /locus_tag="VFO30_04850"
     CDS             5371..6252
                     /locus_tag="VFO30_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9418648.1"
                     /translation="MKGEQRLWSILNDKLDTLRASNRLPEAIRVAESALDLAKRAFSE
                     EDGALAISYEKLGQLLEQQGDRAASKAYLLKAHAILENGKPVDERALFSSARRLAFLC
                     DNLGQSEEAIRFYEKAIAAGSKLDDLPYSDLGTMLNNVALIFRKSGRQKAAEPYYLHA
                     LQIYEKQLGPEHPDVASVLNNLAVFYTNERRYAEAEKIHRRALAIREKVHPPTHPDIA
                     QSKCNLAVVYHSRGDYGRAAELYRASLEAWEQTVETPPEDYEIVASNYADLLRSLGKV
                     RKAQQLEARVRKRRRAS"
     gene            6419..>6840
                     /locus_tag="VFO30_04855"
     CDS             6419..>6840
                     /locus_tag="VFO30_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9418649.1"
                     /translation="MPFQLESNYKPRGDQGQAIAKLIKSVAAGNRHQTLLGVTGSGKT
                     FTIANVVRALDRPTLVISHNKTLAAQLYSEFKQFFPKNAVEYFVSYFDYYQPEAYIPR
                     SDTYIEKDSSINEEIERLRLAATSALLSRRDTIVVASVS"
BASE COUNT         1505 a         1955 c         2028 g         1352 t
ORIGIN      
        1 acgttggcga ggatgtccgc gacacgcgac tcgccgatca acgccatatg tttcgatgcc
       61 ggaccggccg tgagcgtgta gtgaaaattc cagaatgggt ggtcgagtcc ggcaaagaac
      121 tcggctgtcg cggtcgcgct ccgtttgcct aacgaacgtg acaaccccgg ccactcgcac
      181 gccagcatcg ccagcgcggc gacgcggcgc tgcggatggt tgagcgggcg cgtgctgctc
      241 agccgccacg tttcgggcga gagcttgaga cgctgaagtg cgttgcgatg cggccaccac
      301 ttgtcccaga gctggcgcac atattctttt gtcgatcttg cataaacatc caggtccggc
      361 gattcgagga aaccggcgag accgaacagg agcgcttcgc aatcgcccat attttcgcgc
      421 agcaacttca gcggcagccg ttgcgcgacc agggtaaacg gcagtttgtt ttgcttgtag
      481 ccgagtgcgc tcgcgatttc ctggaagagc gcttcgtcgc gcccgtgatt gtcgatcttg
      541 ttgcgcagtc gcgctgcttt tctttgtaac cgaaactgcg cggcagcatc gagcacgctc
      601 cgaacctgtt cttcgggcaa atccttcagc ggcgcctggc aacggcccgg ccgtgcgagc
      661 ggaatattgg cgctaaaggt ttcggggagc acgttgagat cgacgcgcac ctgcaacacc
      721 tcacggttcg atagcgtgcg ggtaaaaaat ttctgcgcgc tttcgttcac gaagacgtgg
      781 agcacggtat tttcaaaagc ggcattggcg gcatgaccgt gggtttccca atttatatcg
      841 gtgagatcaa attcgatgct gccgcgggtc ggctcgcttc catttactcg gatggcggcg
      901 tcgctgaaat ctggtcccgc ttcgcggttc caaatgccaa actgcacgat gtcgatcttg
      961 tcgccggtgg tggcgacgaa atgccggcca aaatctccgg cgaaccagcg cgcctgcagc
     1021 tcaagctcag gcgggacgcg ttggagggga aacagcgcgc gttcgcgcac ccgtgcgaca
     1081 tcgcgcattt gcgcatagcg ctccgccatc ggttctgttg tcatgtcgag cgaagtcgag
     1141 acatctctaa ttgttaacag caagagattc ctcgactccg ctcggaatga cagaaagaga
     1201 gctgaatgtc acgtgtgctt tgcgtttgaa gttgcgagcg gcggttgcta attgcatcca
     1261 tgcttgacat tcgtctcgtg cgcgaaaagc cggacgttgt gcgcgagcgg ctggcgacgc
     1321 gcggcggcgg tgatgaagcg aagatcgaca atctgctgca gatcgatgtc gagcggcgta
     1381 aggcgcagac ggcgctgcaa cagctaaacg ccgagcgtaa ccggttgagc aaggaagtcg
     1441 gcgcgaaaaa gtcgcgcggc gaagcaactg gtgagatcga gacgcaagta cgcaaaattg
     1501 gcgagcaaat caccgatctg agtcagcgca cgagcacact ggatgaggag gaaaagaatt
     1561 tgctcctgca aattcccaac ctgccgcatg cgactgtgcc gtttggaaaa gatcccagcg
     1621 caaatcgtct cgtgcgaagc tggggcgaga aaccgaagct tgaacaagct ctcgatcacg
     1681 tcgcgctggc ggagaagttg aaactcctcg acctcgcgcg cgcggcgaaa ctgagcggca
     1741 gcggattcat atgttttacc ggcgcgggcg cgcggctcga gcgcgcactg attaatttca
     1801 tgctcgattt gcacacgcgc gagcacggtt acgtcgaaat cagtccgccc ttcgttgtcc
     1861 ggcgcgattg catgatcggc acgactcagc tgccgaagtt cgaaccggac atgtatggcc
     1921 tggagaatgg agagctcttc ctggtcccga cggcggaggt gccggtgaca aattttcatc
     1981 gcgaagaagt gattggcgat cttccaaaaa ggttcgtggc ctacacgcct tgtttccgtc
     2041 gcgaagcggg atcagccggg cgcgagaccc gcgggatcat tcgggtgcat cagttcgaca
     2101 aagtggagct ggtgaagatc acaccggtcg agaaatctta cccggagctg gaaacgctgc
     2161 tgaccgacgc ggaacgggtg ttgcaactgc tagaattgca ttaccgcgtt gtcgaacttt
     2221 gcacgggcga tcttgtattc ggttccgcca agacttacga catcgaggtc tggtcgcccg
     2281 gtcaggataa ttatctcgaa gtgtcgagct gctcgaatta cgaagatttt caggcgcgcc
     2341 ggatgcagct tcgttacaaa ggcggcgacg gcaaaaacac gttttgccat acgcttaacg
     2401 gctccggtct ggcgctgccg cggttgtttg ccgcgctaat cgagcacaat cagcagcctg
     2461 acggctccat ccggattcct gacaagttac aaccgtattt cggcgcaagc gaaattcggt
     2521 gaattgtaga ggcgcttgtg ccgagccctg tcactggttc ggcgtttgat acaaacgccg
     2581 ctacaacagt tcgagctggg agcgttcctg ctcggcggca atcttttcaa cttcggccgg
     2641 caacggcaat tcgaagccga gccgttgcgc gaggcgctgc gccgtctcgg gtgcaaaacc
     2701 ttcgaagtga ttgctcgcaa aaacacagac gctgcgcact tccggaagat ggcgctcgat
     2761 tttaagcgac caactctcga gagcggcttc tcgtttccag agcagtttgc tgtagcgatg
     2821 gatgaagagt ccgtccttgt cgtacttggt cgcgtagtcg ccgagcagcc gcaggtaaag
     2881 aaaatcggtg gtgcgcggga ggaactcgaa cggcgcaagg ttgcgctcat tgagctggct
     2941 ggtgtcggcc cagacccagc aaatccggaa cttctcgagc agaccgatga tttgcggccg
     3001 gtgccagccg gggtggcgaa attcaatggc gaagcgaaga tcgcgcggca gttgtttcaa
     3061 aaaattgcgc agtgcgggct tgccgtcttt tggggtgaat gaaggcggca gctggatgag
     3121 aatggcctga agcttcggag tcagcggttc gatcgcacgc agaaatccgt ttagctcggc
     3181 ggaacaatcg cgcagccggc aaacgtgggt gatattgcgc ggcagtttgc aggcgaagcg
     3241 gaaattggca ggtgtcgcct cgacccagcg caagacggta tcttccgcgg gcggatggta
     3301 aaaggtggag tcgatttcga ccgcggaaaa atggcgcgcg taaaattcga gccattgtga
     3361 ttcgggcagc tcagcgggat aaaagctttc ccgccattcg gcgaaactcc aggcgcatgt
     3421 gccgatgcgg attttttgct ggcctgtcag gttcacaaaa gctgaagcaa tggagcgtgg
     3481 gaatcggcaa gtaatgattg gttagcaaac tactcgctga ctccactact ccgatgtctg
     3541 aaacttgacg caaacgcgaa gttcactacc ctgccagacc ccaatggatc tgccagcgcg
     3601 cagtcttgtg ttcctcatcg gtttcgtcgg gttgagcatt ccccagctcg gcacggccgc
     3661 ggtcgtgttc aagcccggcg aaaacgcaaa attggttcca ccgggaattc ttgcgccagg
     3721 cgaggaggag atgaacggca atgcgcagga actctttcag aaaggacagg ccgccgaacg
     3781 agcggggaat ttgggtagcg ccatccgggc ttacagggct ctggtgcggc ggtacccgcg
     3841 cgatgcgctc gcggccggtg cacttttcaa aatggcagag ttgcaggagc aatcgcacca
     3901 atatctcaaa gcggccgtga cgtaccgcgc actggtggaa agatatccga gcagcgaccg
     3961 atttaacgaa gcgatcgaag cgcagtttcg catcggcgag atgtacctgg ccggcaaaaa
     4021 gcagagacta ctgggaatcc cgatcgggtc cgcgttggag cgtgccgtgg agattttcgc
     4081 ctccgttgtc cgaactgcgc cttatggcaa gtacactgcg cgcgcccaat tcgacatcgg
     4141 attggtgcga gagaaacaag gcgccaagga cgcagcgctc gaagcgtacc aggcggtggc
     4201 cgacaagttt ccgaacgagc cggtcgcagc cgacgcgcag tatcaggtcg gttacatctg
     4261 gttcacggcg gcgcgccagg gcgccagaga cattgccgcg acacacaatg cgaaaactgc
     4321 ttttgaagat ttcctcttcc gccatccgaa cagcgaaaaa gccgcccaag ctcgcgcaaa
     4381 tctgcagctg ctggaacaca agcaaacaaa ttcgtcctat gaagtggcga agttttacga
     4441 caaacagaag aaatatcgcg cggctgcgat ctactacaac gaagtgattc gccagcagcc
     4501 tggctcagcc gaaagcagcc aagcgcagaa acgaatcgat caattgcgcg ccaagtacgg
     4561 cgacgcggcg ttgcagccgc gcgccggcga catcggagcc gagcagaaaa aaaccagcga
     4621 gcgcgacagt ggggtggcaa agaacgcgag cggcgagccg ccaggcgcaa gcagcgtcaa
     4681 gagcaatgcg cccttgccgc caagtgacgc cgatacggct ctgcccccgc ccgcctcgct
     4741 ccttcccgac acgaccactg cgccagcaac gctggacacc gacagcacga cgacatcgtc
     4801 gccgtctcgt ggtgcctcgc cggcccccgc ttcccccgac gcttctgctt cgcccagccc
     4861 gtgaaatcgc tcatcgcggt ggcgctgacg gcgtcatgtc tgagtggttg tctcgggtat
     4921 cacatcgggc cggtaaagcc gtactacctg cgcgatattc actcgattgc tgtccccacg
     4981 tttaccaaca ggacgcttct cccgcgtgtt gaggttttgg tgaccgacac ggtgatcaaa
     5041 caattgcagc aggacggcac tttcgaagtc gcgggtgaag acaaagcgga tgcggtgttg
     5101 aagggtgaga tcacgcacgt agaacgtgcg ccggcgcggc cggtccgcgg taacgttctc
     5161 gcgaccgccg aattcgctct tactgtttac ctgaaataca gcgtggtaaa caaaagcggg
     5221 tcgcaactcg ccgtcaacga gacgtatggc agcacgactt tttttgtcgg caccgacgtg
     5281 atcaccgacg agcgtcaggc gctgccattg gcgaccgcgc aattggcgac caaccttgtc
     5341 gcacagctaa ccgagggttg gtgatgcaag atgaaaggcg aacaaagact ttggtcgatt
     5401 ctcaacgaca agctcgatac tttgcgtgcc agcaatcgcc tgcccgaggc gatccgcgta
     5461 gctgaaagcg cgctcgatct ggccaagcgc gccttcagtg aagaagatgg cgcgctcgcc
     5521 atcagctatg aaaagctcgg ccagcttctc gagcaacaag gcgaccgcgc tgcgtccaag
     5581 gcatacctgc tcaaagcgca cgctattttg gaaaacggaa aaccggtcga tgaacgcgcc
     5641 ctctttagct cggcgcgacg gctcgccttt ctctgcgaca atctcggcca atccgaggaa
     5701 gcaattcgat tttatgaaaa ggcgatcgcg gccggttcca aattggacga tctcccctac
     5761 tcggacctcg gcacgatgct caacaacgtt gcactcatct ttcgcaagtc cggtcggcag
     5821 aaagcggcgg agccgtatta cctgcacgcc ctgcaaatct acgagaaaca gctcgggcca
     5881 gagcatcctg atgttgcttc ggtcctaaat aatctggctg tgttttacac gaatgaacgc
     5941 cgctacgccg aagctgagaa aatccatcgg cgcgcgcttg ccattcgtga gaaggtgcac
     6001 ccgccgacgc accccgacat cgcgcaatcg aaatgcaatc tggcggtcgt ctatcattcg
     6061 cgcggcgatt atgggagagc cgccgaactc taccgcgcct cacttgaagc ctgggaacaa
     6121 accgtcgaaa caccgcctga agactacgaa attgtagcgt cgaactacgc ggatttgttg
     6181 cgttcgctcg gcaaagttcg caaggcgcag cagctcgagg cgcgcgtccg caagcgccgg
     6241 cgcgccagct aattgccact gcaccatcga catgttcgtc gcggcctgcg cacctgatgc
     6301 aactcgactg ctttaagcag tcgagttttg ccgccgcggt atttgctctc ctccgcctgg
     6361 cacagctccg tcgcgcgcgc tctctttgga ctgctggcgg gtgatgctag attgcctgat
     6421 gccgtttcag ctcgaatcaa actacaaacc gcgcggcgat caggggcagg caatcgcgaa
     6481 attgattaaa tcggtcgcag ccggtaatcg acaccaaaca ttgcttggtg tcaccggttc
     6541 gggcaaaacg ttcaccatcg cgaatgtcgt tcgcgcgctc gatcgtccga cgctggtgat
     6601 ctcacacaac aagaccctcg ccgcgcagct ttactccgaa ttcaagcaat tctttccgaa
     6661 gaacgcggtc gagtattttg tgagttattt cgattactac cagccggaag cctacatccc
     6721 gcgcagtgac acttacatcg agaaagattc gtcgatcaac gaagagatcg agcgcttacg
     6781 cctcgccgcg accagcgccc tgctctcacg ccgcgatacc attgtcgtcg cgagcgtctc
//
LOCUS       DASTDG010000149         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_469671, whole genome shotgun sequence.
ACCESSION   DASTDG010000149 DASTDG010000000
VERSION     DASTDG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469671"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFO30_04860"
     CDS             <1..582
                     /locus_tag="VFO30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9418650.1"
                     /translation="HIVDLTIERMPLSIARTGYTGEDGVEVFFRAEDAANVWNLVLET
                     GERFGIKPCGLGARDTLRLEMCYPLNGSDLSPEHNAIEAGLGVFVDLAKPNFIGRDVL
                     LQTKENGPREKLVPFRMKEKGPPPRPHYSVFENGERIGEVTSGTLSPSLQRGIGMAYI
                     DAPRAKVGAKIDIEIRRQKFPAVIEKKPLYKKT"
     gene            579..959
                     /gene="gcvH"
                     /locus_tag="VFO30_04865"
     CDS             579..959
                     /gene="gcvH"
                     /locus_tag="VFO30_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9418651.1"
                     /translation="MNIPDDLLYTETHEWIRREGDNIRVGITDHAQSELTDVVYVELP
                     KLDREANAKEAVAVVESVKAASDIYAPVKGKVVEANKALEADPGLINREPYGQGWIFA
                     LKIENADDLKQLKDAAAYGGQIAG"
     gene            complement(963..1343)
                     /locus_tag="VFO30_04870"
     CDS             complement(963..1343)
                     /locus_tag="VFO30_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418652.1"
                     /translation="MHSPKKVLLIDREPGITRVVRQALETAGKCLVNEQRDKFSMDAA
                     RAFEPDLILFDMPANSITGQTLERWIQTDTILRDTPLVHLSSLKSETQIMSGGILSGY
                     SFFAGPIGIDDIIRGVEQLLFGTD"
     gene            complement(1398..2072)
                     /locus_tag="VFO30_04875"
     CDS             complement(1398..2072)
                     /locus_tag="VFO30_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HET9418653.1"
                     /translation="MTRLAALISRQDARHRVLIALAVAVAVSFLLRGRVHFATEMIAT
                     WDAFAFCVLLLAWFTILTTPIRRLRERAKEQDLSRLLIFFFVVVAACAALFAVAFLIS
                     INKAELQGHVTTHLLLALGTVIFSWSLVHTVFGLRYAHTFYGESDDPAQDRQAGGLIF
                     PEGHVPDYFDFAYFSFVVGMTFQVSDVQITSRRMRRLTLLHSILSFGFNTVILALLIN
                     TVSSLL"
     gene            2174..2485
                     /gene="xseB"
                     /locus_tag="VFO30_04880"
     CDS             2174..2485
                     /gene="xseB"
                     /locus_tag="VFO30_04880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9418654.1"
                     /translation="MSSKPKPAGAELNFESAMDRLEAIVGKMESGKLPLEELIVRYEE
                     GMNLVKICQERLASAEQKIEIIGRDNAGKPVVKDFEPAAEAKIANHSREEPKNDDVSL
                     F"
     gene            2463..4391
                     /gene="dxs"
                     /locus_tag="VFO30_04885"
     CDS             2463..4391
                     /gene="dxs"
                     /locus_tag="VFO30_04885"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9418655.1"
                     /translation="MTTLVSSETSSARKRLLDGIRSPADIKALREEDLLQLAQEVREE
                     LIRVLSQTGGHLGPNLGVVELTIALHRVFETPRDRFVMDVSHQGYVHKMFTGRLDRFS
                     TMRQYGGLNGFLLRSESEHDCYGAGHAGTALSAALGMAVGRDLNDTNENIVVIAGDAA
                     FTCGISYEALNNVKTSTKRFIVVLNDNEWSIAKNVGAIANYLNRIVTSPMFAHLHEKA
                     RDFVRAIAGKSVVHFAHKVEESVKGLIVPSVIFEELGLRYYGPIDGHDIPLLIRTFEF
                     LKTQDEPVLLHILTKKGKGYEPAIKQPDKFHGLGKYKIESGETAPTPTPTYSEVIGKT
                     LAKFADTNQKLVAITAAMPSGTGLSHFAKAHVDRYFDVGIAEEHAALFACGLATQGLT
                     PFLTIYSTFFQRAYDMAIHDMAIQKLNVRLCMDRAGLSGDDGPTHHGLFDIGYLRHVP
                     NWVHMQPRNEDEFVDMLWTMAHYNSGPIAIRYPRGSGPGAQIKPEPRLLEIGKAEVVQ
                     HGRDVAIFGLGNMFEMAEEAARKLEEKGISVALINPRWIKPLDTGTLEFFARSVEVVC
                     TIEDHVLHNGFGCAVMEHLHSQMINTPVVRIGWPDEFIEHGTIPILRKKHGVTADALV
                     EKVLPLLRRKPKAQPSVA"
     gene            4814..5830
                     /locus_tag="VFO30_04890"
     CDS             4814..5830
                     /locus_tag="VFO30_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9418656.1"
                     /translation="MSAIETRELTKVYRTYRKERGLWGSIKGLARRRYDETRAADRVS
                     FEIDHGEFVGFLGPNGAGKTTVLKMLSGLLNPTSGDARVLGFVPWERRDEMKRQFSLL
                     MGQKNALWWDLPAQESLELNRAIYGIDRARFNQVVGGLSELLEVQDKMNVMVRELSLG
                     ERMKMELIAALIHEPRVLFLDEPTIGLDVVSQKRVREFLRIYNEEHRIVTLLTSHYMQ
                     DIEELCHRVLVIDHGKIFFDGPLDDIIDRFSGHKILSLTFDKPVKRDFSRFGEVIEQT
                     PMSVQLKVPRAQVTETCRQLLDCCDVSDINVQEVPVEEVIRQLFGERKESQRALVAAD
                     ASAV"
BASE COUNT         1381 a         1583 c         1784 g         1162 t
ORIGIN      
        1 cacattgttg atctcacaat tgaacggatg ccactctcga tcgcacgcac cggttacacg
       61 ggcgaagatg gcgtggaagt tttctttcgc gcggaggacg cggcgaacgt ttggaatctc
      121 gtgctggaaa caggagaacg attcggaatt aaaccgtgcg gcctgggcgc gcgtgacact
      181 ttgcgactgg aaatgtgtta cccgctgaac ggctccgatt tgtcgccgga acataacgcg
      241 atcgaggcag gccttggcgt tttcgtcgac cttgcgaagc caaacttcat cggccgtgac
      301 gttctgttgc agacgaaaga gaacggcccg cgcgaaaagc tcgtgccgtt tcgtatgaaa
      361 gagaaaggtc ctccgccgcg gccgcattat agcgtgttcg aaaacggcga gcgtatcggc
      421 gaagttacga gcggcacgct ctcgccaagt ttgcagcgcg ggatcggaat ggcttacatc
      481 gatgcgccgc gtgcaaaagt gggcgcgaag atcgatatcg aaatacgccg gcaaaaattc
      541 ccggcggtga tcgagaaaaa accgctctac aaaaagacgt gaacattccg gacgatttgc
      601 tttacaccga aacgcacgaa tggattcgtc gcgagggcga caacatccgc gtcggtatca
      661 ccgatcacgc gcaatcggag ctcacggacg tagtttacgt tgagctgccg aagctggacc
      721 gtgaggcgaa cgctaaagag gcggttgcgg tggtggagtc agtcaaggcg gcgagcgata
      781 tttacgcgcc ggtgaaaggc aaagtggtcg aagcaaacaa ggcgctcgaa gccgatcccg
      841 gcctaatcaa tcgcgagccg tacggccagg gctggatttt cgcgctcaag atcgagaatg
      901 cggatgatct gaagcagtta aaagacgcgg ccgcttacgg cgggcaaatc gccggctaac
      961 cttcagtcgg tgccgaaaag caactgttcg acgccgcgga tgatgtcgtc gatgccgatt
     1021 gggccggcga agaaactgta gccgctgaga atgccgccgg acatgatttg cgtctccgat
     1081 ttcaggctgc tcaggtgaac cagcggcgtg tcgcggagga tggtatcggt ttggatccag
     1141 cgctcgagcg tttgaccggt gatgctattg gcgggcatgt caaagaggat caagtcgggt
     1201 tcgaaggcac gcgccgcgtc catggagaac ttatcacgct gctcgttcac cagacacttg
     1261 cccgctgttt cgagcgcttg cctaacgacc cgagtgatgc ccggctcgcg atcgatgagc
     1321 aatacttttt tgggcgagtg catggaagtc atcgcaagca tcggttgtgc cagagcgaag
     1381 cggcaccggg ccgcgcctca gagcaaactg gagacggtat taattagcag cgcgagaatg
     1441 accgtgttga atccaaacga gagaatgctg tgtagcagcg tgaggcggcg cattcggcgc
     1501 gatgtgatct gcacgtcgga cacctgaaag gtcatgccga cgacaaaaga aaaatacgca
     1561 aagtcgaagt agtccgggac gtggccctcg ggaaagatga gtccgccggc ctgtcgatct
     1621 tgcgcagggt catcgctctc gccgtaaaag gtgtgggcat atcgcagtcc gaacaccgtg
     1681 tgcaccagcg accatgaaaa aatcacggtg ccgagcgcga gcagaagatg cgtagtcacg
     1741 tgtccctgca attcggcttt gttgatgctg attaaaaagg cgacggcaaa aagcgcggcg
     1801 cacgctgcga ccaccacaaa aaagaaaatc agtaggcggc tgaggtcttg ttctttggcg
     1861 cgttcgcgga gtctacgaat aggcgtagtg agaatggtga accacgcgag gaggaggacg
     1921 caaaacgcga acgcgtccca tgtcgcgatc atttcagtgg cgaagtggac gcggccacgg
     1981 agcaaaaagg agacggccac ggcgacggcg agcgcgatga gcacgcgatg gcgtgcgtcc
     2041 tgtcgcgata tcaacgccgc gagcctggtc atagcggtcg cgagtaaata aacatccacg
     2101 cgagcaggca agccgcattc gcgagctgcc gcttttcatc gcgagtagcg tgtgcttaat
     2161 tgcgcgtttg atcatgagca gcaagccgaa gccggccggc gccgagctga actttgagag
     2221 cgcgatggat cggctggagg caatcgtggg aaaaatggag tcgggcaaac tgccgctgga
     2281 agagttgatt gtccgctacg aagaaggaat gaatctggtg aagatttgcc aggaacggct
     2341 cgccagcgcc gaacagaaga tcgagatcat cggccgcgac aacgccggca agccagtggt
     2401 taaagatttc gagccggcgg ctgaagcgaa gatcgccaat cactcaagag aggaaccgaa
     2461 gaatgacgac gttagtctct tctgagacca gcagcgcgag gaaacggctg ctggatggaa
     2521 tccgttcgcc ggctgatatc aaagctttac gcgaagaaga cctcttgcag cttgctcagg
     2581 aagtacgcga ggaactgatt cgcgtgcttt cgcagaccgg cggtcatctt gggccgaatc
     2641 ttggcgtggt tgaactcacg atcgcgttgc accgcgtatt cgagacgccg cgcgaccgtt
     2701 ttgtcatgga cgtgagccac caaggctatg tccacaaaat gttcactggc cgactcgacc
     2761 gcttctcgac aatgcgacag tacggcggac tcaatgggtt ccttctgcgc agcgaaagcg
     2821 agcacgattg ttacggcgcg ggtcacgccg gtaccgcgct atccgcggcg ctgggaatgg
     2881 cggtcggccg cgatctgaat gacacgaacg aaaacatcgt cgtcatcgcg ggggatgccg
     2941 cgttcacctg cggcatcagt tacgaagcgc tcaacaatgt taagacaagc acgaagcgtt
     3001 ttatcgtcgt gttgaatgat aacgagtggt cgattgcgaa aaatgtcggc gcgattgcga
     3061 actacctgaa tcggatcgtg acgagtccaa tgtttgcgca tctgcacgag aaggcgcgtg
     3121 atttcgtgcg agcgattgcc ggtaaatcag tcgttcattt cgcgcacaag gttgaggaaa
     3181 gtgtcaaagg tctcatcgtg ccgagtgtca tattcgaaga gctcgggttg cgctactacg
     3241 gcccgatcga cggacacgat atcccgctgc tcatccgaac gtttgaattc ctcaaaacgc
     3301 aggatgaacc ggtgctgctg cacatcctga cgaaaaaggg caaaggctac gaaccggcca
     3361 ttaagcagcc ggacaaattc cacggcctcg gcaaatacaa gatcgagagc ggcgagaccg
     3421 cgccgacccc gaccccgacg tactcagagg tcatcggtaa aacgctggcg aaattcgccg
     3481 acacgaatca aaaactggtg gcgatcacgg ccgcgatgcc cagcggcacc ggtctctcac
     3541 attttgcgaa agcgcatgtg gaccgctact tcgacgtcgg catcgccgag gaacacgccg
     3601 cgctgttcgc gtgtgggctg gcgacgcagg gactcacgcc atttctcacg atctactcta
     3661 cctttttcca gcgggcttac gacatggcca ttcacgatat ggcaatccag aaacttaacg
     3721 tccgcctctg catggaccgc gcgggtttga gtggcgacga tggcccgacg catcacgggt
     3781 tgttcgatat cggttacctt cggcatgtgc ccaactgggt ccacatgcag ccgagaaacg
     3841 aagacgagtt cgtcgatatg ctgtggacga tggcgcatta caactccggg ccgatcgcca
     3901 ttcgttatcc gcgcggatcc ggtcctggcg cgcaaatcaa gcccgagcca cgcctgctcg
     3961 agattggtaa ggcggaagtc gtgcaacacg ggcgcgacgt ggcgatcttt ggcctcggca
     4021 acatgttcga gatggccgaa gaagcagcgc gcaaattgga ggagaaaggc atctccgttg
     4081 cattgatcaa cccccgttgg atcaagccgc tcgataccgg cacgcttgag ttcttcgccc
     4141 gcagtgtcga ggtcgtctgc accatcgaag atcacgtctt gcataatgga tttggttgcg
     4201 ccgtgatgga gcatcttcat tcacagatga tcaacacgcc ggtcgtgcgc atcggttggc
     4261 ccgacgaatt catcgagcac ggcacaattc ccattctgcg caaaaagcat ggggtcaccg
     4321 ccgacgcgct ggttgagaaa gttctgccac tgctgcgcag aaagccgaag gcgcaaccat
     4381 cggtggcctg aacaaaggat gaaggatgaa ggatgaggga tgagcgatga aagggacgat
     4441 ttgcgacagc gaactaagaa attcgcgctg cggatcctta aactctacga gacattgcct
     4501 cgcgctggct ccgcacaagt actaggtcat caacttctca aatccggaac ttcggtcgga
     4561 gcaaacttcc gcgaggcaca tcgcgcgcgc tccgatgcag agttcgtctc caaagttgga
     4621 gattctctga aagaactcga agaaaccaac tattggctgg agctgcttgt cgaatcggga
     4681 gcagtcagcc acaagaatct ggcgtcttta attgacgaga cgtcgcaact gactgcgatt
     4741 ctaacctgca tcgcgaaaaa gattcgcgcg cggctttagt ttcatccttc atccctcatc
     4801 cttcatcctt tccatgtctg cgattgagac tcgcgaactg acaaaggtct atcgaaccta
     4861 ccggaaggag cgtgggctct gggggtcgat caagggtttg gcgcgtcgac gctatgatga
     4921 aacacgcgcg gccgaccgcg tttcgttcga gatcgaccac ggcgagtttg tcggctttct
     4981 cgggccgaat ggcgcgggca aaacgaccgt gctgaagatg ctttctggtt tgctcaaccc
     5041 aacctccggc gacgcgcgcg tccttgggtt tgtgccgtgg gagcgccgcg atgagatgaa
     5101 gcggcagttc agtttgctca tgggccagaa gaatgcgctt tggtgggatt tgccggcgca
     5161 ggaatcgctc gagctcaatc gcgcgatcta cgggatcgac cgcgcccgct tcaatcaggt
     5221 ggtcggcggc ttgtccgagc tgctcgaggt gcaggacaaa atgaacgtga tggtgcgcga
     5281 gctttcgtta ggcgaacgca tgaagatgga gctgatcgcg gcactcattc acgagccgcg
     5341 cgtcttgttt ctcgacgagc cgacgattgg gctggacgtg gtcagccaaa aacgggtgcg
     5401 cgaatttttg cgcatctaca acgaagagca tcgcatcgtc acgctgctca ccagccatta
     5461 catgcaggac atcgaggagt tgtgccatcg tgttctggtt attgatcatg gcaaaatttt
     5521 ctttgacgga ccgctggacg acatcatcga ccgcttctcc ggccacaaaa tcctcagcct
     5581 cacattcgat aaaccggtga agcgcgactt ctcgcgtttc ggcgaagtga ttgaacaaac
     5641 accgatgtcg gtgcaactca aagtgccgcg cgcacaagtc actgagacct gccggcaact
     5701 gctcgactgc tgcgatgtga gtgacatcaa cgtccaggaa gtgccggtgg aagaagtgat
     5761 ccggcagctc tttggcgaac gaaaagagag ccagcgcgct ctggtcgcag ccgacgcttc
     5821 ggccgtttag taacgccggc tcagagcttc gcgcaattcg tgtgcgtaag ccggattcac
     5881 ctcgaacgcc gtcactggca tctcggcgag
//
LOCUS       DASTDG010000150        12561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_188411, whole genome shotgun sequence.
ACCESSION   DASTDG010000150 DASTDG010000000
VERSION     DASTDG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188411"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFO30_04895"
     CDS             <1..200
                     /locus_tag="VFO30_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418657.1"
                     /translation="QVTSTVRWLDCVERLVELGCEQFVELGPGGVLGGLLKRTRQDVD
                     VISVADAESVRECSERISASR"
     gene            310..2007
                     /locus_tag="VFO30_04900"
     CDS             310..2007
                     /locus_tag="VFO30_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9418658.1"
                     /translation="MSGPAELVMAALLGGLLLALNAINILHRRFDSDEMGHLHIVWSW
                     THGFVQYRDVFDNHMPLFHLLFAPVLALIGDRATILYWMRLILLPMYFVTAWCTYAIA
                     TRLFSRRAGAWSVLALGFYASYHLVAFEFRTDNLWTPLWLLCVTSLLTRAITVRRSLV
                     AGVLLGFCFAVSMKSTIFLLSLAISFPLAIALVRTRNAGQERIYFARCAATFLAGSIL
                     VPGTIAVFFAVKGVWRDFRYGVLDFNFLADQIYKNQLLYNRHPVLALVVLVSTLAAAI
                     YIGQRIVSACGDRDVAFRRTFVFFICVFYIAFFKILWPPISRSYSGFYPLASALLVGG
                     IFAASRRLDAYGWKFKPILRPATLLPCIVLAEMVFLLSLHPIRKNGTQEETRLLRNVL
                     ALTEPTDYVFDCKGETVFRPRCFRPILEAITIRAIRRGIMVDDAPERCIGTHTCVVAT
                     MLGKRFSSSTRQFVLHNYLPVAKDLRVAGMVLHASPQDPHRLNFQVVVPADYEIMEAD
                     RTVSGTLDGSPYQGARFLSAGPHSFESESGSDDLILLWAKAIDRHFPCLATRSSPHG"
     gene            2105..2557
                     /locus_tag="VFO30_04905"
     CDS             2105..2557
                     /locus_tag="VFO30_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9418659.1"
                     /translation="MALAHQIDKDLQDAMRAKDAPRLSVLRMLKAALKNAAIEKSGAD
                     AQLSEADAIQVIRKQVRQRQDSIESFEKGGRAELAAKEREELSILQAYLPQALSADEL
                     AKVVRETIAEVGATSKAQMGAVMKALQAKVAGRADGKTLSAEVQRQLS"
     gene            2642..3325
                     /gene="ispD"
                     /locus_tag="VFO30_04910"
     CDS             2642..3325
                     /gene="ispD"
                     /locus_tag="VFO30_04910"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9418660.1"
                     /translation="MLSAIIVAAGASERMGFDKLFAPLADKPVVAHTIDAFERTSCVD
                     EIILVGRKDSLEELRKVLGANTKVRHLVPGGTLRQDSVRAGLQQVDARADYVAVHDAA
                     RPLITPEKIARVFGVAREHGAAALAEPISDTLKRVDVDLAVSESVDRDRLFAMQTPQV
                     FARQLLIDAYDALAAENISVTDEVSAVQHLGRKVVLVPNQDFNFKITYPRDFLLAEFI
                     FRHRTREAG"
     gene            complement(3322..4293)
                     /locus_tag="VFO30_04915"
     CDS             complement(3322..4293)
                     /locus_tag="VFO30_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9418661.1"
                     /translation="MIIKPSSLGDVVHTLPAVALVRDAHSDAEITWVINPEFAPLLRG
                     SPHVDHVHIFPRGEFRGLGAPSSLGRWIKTTGKLRPDLALDFQGLLRSALIARISGAK
                     EIYGMSDAREGARWFYHRRAKVNRHEHAIERYLKLAECAGATVTDQLRCPLPSGDVLP
                     RFEQYPPFILLHPFARGSGKSLSNRVIEEFCRALSPTRVVVVGKSNRKIDITDNCVEL
                     TNQTSLLQLIWLIRAASFIITVDSGPMHIAAAITDRVLSIHTWTDPRRVGPYNPDAWV
                     WKNEKLLRVSELESSQGLRRSRKFKAKHVPAVVELVRPLVPIDPMFV"
     gene            complement(4341..5063)
                     /locus_tag="VFO30_04920"
     CDS             complement(4341..5063)
                     /locus_tag="VFO30_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9418662.1"
                     /translation="MRFAVFSDIHSNLEALEAVLDDAGQQSCTDYVCLGDVVGYNANP
                     HECVERVRELDCPIVKGNHDEQASLPESSRDFNPLAEHAMQWTRDNLTEDDKDWLREL
                     RFERQVRDFTIVHVTLDTPSQWGYVFNILDAAASFTYQHTTVCFFGHTHVPMAFVRDG
                     GVSRLKTDRLSIDATKKYFINTGSVGQPRDADWRAAYCVYDTDRNLVEQRRVSYKLAL
                     AQQKIIKSGLSPLLAERLALGR"
     gene            complement(5147..5914)
                     /locus_tag="VFO30_04925"
     CDS             complement(5147..5914)
                     /locus_tag="VFO30_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418663.1"
                     /translation="MTRLVITSCALLLFGAIKLPIERDLAAQHRQERLRGVEFNLDLR
                     EKLGQLGFVAALSGFRAAVADVLFIQAHVAWEQTEWGRVLLLFRQVTTLQPRSILFWD
                     MAAWHMAWNASVAAMKDPTQPRLALRVKAQREYFALGKDFLERGIANNPDKPQLYEVL
                     GRLYKDKYNDHEHAAEFFAKAAGLPRAPSYDKRFAAYELSYCEGREQEAYARLRQLYD
                     MGPQERLPTLISRLKLLEDKLNIPPDQRIPDQNRLKR"
     gene            complement(5911..6798)
                     /locus_tag="VFO30_04930"
     CDS             complement(5911..6798)
                     /locus_tag="VFO30_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9418664.1"
                     /translation="MHAIRHRAFSFRRVYAITTNTLTELTRLRVFYVLLIFALLLIGS
                     SIFVARLTFQQEFQILKDVSLGAMSIFTSLLAIVATARLIPQDVEDRTIYTILAKPVP
                     RIEYILGKIAGVLLLLLISTIVMSAMFLAVLYAREQVLLHETARQMSAAPHEQLEDAL
                     RGVRASAWNVNLVPGITVIFLKACLLAGLTLFVSTFATTSIFTIVVMVFVYFIGHLQA
                     TAREYWLQEHAATWTTRIFLALVALIFPDLQSFNLVDDVVAGAAIPAALFAKTAALGV
                     FYTTIYTFLAAFVFYGKEL"
     gene            complement(6801..7703)
                     /locus_tag="VFO30_04935"
     CDS             complement(6801..7703)
                     /locus_tag="VFO30_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9418665.1"
                     /translation="MTDLPRRSGAEAGAAVAVRGLTKVFPVPLHRQSVVAVCDLNLRV
                     EPGEVYGLLGPNGSGKSTTLKIILGLVSPTRGRTEIFGRDSTLVDSRAAVGFLPENPY
                     FYKYLSGEETLRFFGKLCGLRAAALQKRVDELLELVGLTNARNRRLGTYSKGMLQRIG
                     LAQALINEPKLVVLDEPTAGVDPAGSRDIRNLIVGLRGRGITVLLSSHLLAQAQEICD
                     RVGILANGLLVREGRLDELLAVENQTELVLENASDRLIDEITAVAAKSNARVLAHRTS
                     TTTLERLFLDATQGNQTGSTSVEI"
     gene            complement(7696..7971)
                     /locus_tag="VFO30_04940"
     CDS             complement(7696..7971)
                     /locus_tag="VFO30_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET9418666.1"
                     /translation="MTSLQVFFEGNVQGVGFRWTVRNIAKGFAVTGWVRNLLDGRVEM
                     RVSGEDAEVRAFLDAVMDSELRSHIRKHTEQKLEQLVAARGFEICHD"
     gene            complement(7975..8409)
                     /locus_tag="VFO30_04945"
     CDS             complement(7975..8409)
                     /locus_tag="VFO30_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418667.1"
                     /translation="MKLRLSILLLSILLACGSPGWAQSDREVDARKNALELAGAFTND
                     GFKMRDGHWCGLLRPHDMALIAVNLYAGNQYWFSVGAVEPAKKITVNLYDEGGKQVPA
                     EKYDSGAKAAAGFSPTGSGQYFVAVDLLEGAESNYCLVYSYK"
     gene            complement(8406..9218)
                     /locus_tag="VFO30_04950"
     CDS             complement(8406..9218)
                     /locus_tag="VFO30_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418668.1"
                     /translation="MKRLMRAVAIATLIACYCVIGAEPARLSPDRLARAIKAEEISIP
                     TPGELFAALEKPGKPNWSGQYREPIPMTYRNRAQIALNLGGLIADGFVAVEAKDSQQV
                     KNIGSDIIKLSKALGVSENLLRRGNSLNDFAENDEWDALQEELEATQNEVKSSMQTHS
                     DQDLIILVSVGGWLRGTQVVTSAIMENYDERAAKVLRQPALVSFIHSKVNGISPELRD
                     EPLVKHVDDQLVSIEKLVSFAPGKAPTPDEVRKVNEAVGRTIESIQGKPDAR"
     gene            9317..10600
                     /gene="eno"
                     /locus_tag="VFO30_04955"
     CDS             9317..10600
                     /gene="eno"
                     /locus_tag="VFO30_04955"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9418669.1"
                     /translation="MGKTTIAEVRAREILDSRGNPTVEVDVRLESGGLGRAAVPSGAS
                     TGEHEAWELRDGDKRRYGGKGVSHAVASVNEKIAREIRGLDARDQAKIDNRLIELDGT
                     PNKNNLGANALLGVSLAVAHAAAAAENLPLFRYLGGGEARVLPVPMMNILNGGVHSDA
                     PIDFQEFMIVPRGAPTFAEALRYGAEVFHTLKRVLQDRHLSTAIGDEGGFAPQLNSAE
                     DALETIAAAVEKAGYSLGNQVFIALDPASSEFFDAERGCYVFKKSGGAEKSVEELIQY
                     YVDLCRRFPIISIEDGCAENDWDGWKKLTARLGHQIQLVGDDLFVTNVKFLRRGIEEH
                     VANSILIKVNQIGTLTETLATIELAKKNNYTAVISHRSGETEDTTIADLAVATNAGQI
                     KTGSLCRSDRVAKYNQLLRIEEELGDKAVYGGKLR"
     gene            10602..10961
                     /locus_tag="VFO30_04960"
     CDS             10602..10961
                     /locus_tag="VFO30_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9418670.1"
                     /translation="MEQSYGDFRARREATVWQRLNRVLAVLLVLAVWLVIISLFVPPY
                     KRLMQSRAEIDNLQTRVDEQKKLLGRRSHEVTLLQTDATYLETIARDRLDLMKEGETI
                     FRLEPTQPGKGRPPARP"
     gene            11174..12217
                     /gene="prfB"
                     /locus_tag="VFO30_04965"
     CDS             11174..12217
                     /gene="prfB"
                     /locus_tag="VFO30_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9418671.1"
                     /translation="MPRLQQNLTALEARMSEKNFWDNRERAQADVEELSRLRSLINPF
                     HELEREISDFEALQQLATEEQDESHRVQAERELAAEHERLLHKLNEFELRQFLSGEND
                     RANAFLTIHSGAGGTESCDWADMLLRMYQRWIERNGFKSQVIDVQTGEEVGIKSVTLL
                     VTGDYAYGYLQTERGVHRLVRISPFDANKRRHTSFASVDVVPEIADTAPIEINLADLE
                     IDTFRSGGKGGQNVNKVETAVRIVHKPTGIVVACQAERSQGRNRDLALKMLKAKLYQI
                     EEDKKRAEIDRQYGEKGEIAWGNQIRSYVFQPYQMVKDHRTGAETSNVQEVMDGNIGM
                     FIQAKLRGEKGKE"
     gene            complement(12267..>12561)
                     /locus_tag="VFO30_04970"
     CDS             complement(12267..>12561)
                     /locus_tag="VFO30_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418672.1"
                     /translation="LAHLLAQFRAGRRRILPHGPEWRDACVDLGIADEARCHNLPFTA
                     RTFPTRFIYRCPNCRREFPRVRRIRRTVACLRCCRAYNHGEFDPRFRLQLVKT"
BASE COUNT         2695 a         3550 c         3669 g         2647 t
ORIGIN      
        1 aacaagtcac gagcaccgtc cgctggctcg attgtgtcga gcgactggtc gagcttggct
       61 gcgagcaatt tgtcgagctc ggaccgggcg gcgttctcgg cggtttactc aaacgtacgc
      121 gccaagatgt cgatgtaatc tccgtcgcgg acgcggagtc ggtgcgcgaa tgtagcgaaa
      181 gaatttccgc atcgagatag gcagaagcag gtttcaatca gcatgttttg gggctttgga
      241 aaagcacgag ctcctttggc aatgccgaaa cggatatctc tcgcgcaacg tgccgcggat
      301 tatttcgaaa tgtcgggacc ggctgagctg gtgatggccg cgcttctggg cgggcttctt
      361 ctcgctctaa acgcgatcaa catcttgcat cgccggttcg acagcgacga gatggggcat
      421 ctgcacatcg tttggagctg gacgcacggc tttgtccagt atcgcgacgt gttcgacaat
      481 cacatgccgc tttttcatct gctctttgcg ccagtcctcg ccctgatcgg cgaccgcgca
      541 accattcttt actggatgcg tctcattctt ctgccgatgt atttcgttac ggcatggtgc
      601 acttacgcga tcgcgacgcg gcttttctct cgccgcgccg gcgcgtggtc agtgctcgcg
      661 cttggctttt acgccagcta tcacttggtt gcgtttgaat ttcggacgga caatctctgg
      721 acacccctct ggctgttgtg cgtcaccagt ttgttaactc gcgcgattac agttcgccgc
      781 tcgttagttg ccggtgtgct gctcgggttc tgtttcgctg tctcgatgaa gtcgacgata
      841 ttcttgcttt cattggcgat cagctttccg ctggcgatcg cgctggtgcg cacgcgcaac
      901 gccggtcagg agcgaatata cttcgctcga tgcgccgcga cctttttggc cgggagcatt
      961 ctcgtgccgg ggacgatcgc tgttttcttc gcggtgaaag gcgtttggcg agattttcgt
     1021 tacggcgtgt tggattttaa ctttttagcc gaccaaattt acaaaaacca gctgctgtac
     1081 aacaggcatc cagtattggc gctggtggta ctggtaagca cactggccgc tgcgatttac
     1141 atcgggcaac gaatcgtctc tgcttgcggc gatcgcgatg tcgcttttcg ccgcactttc
     1201 gtctttttca tctgcgtctt ttatattgcg ttttttaaga tcttatggcc gccgatatct
     1261 cgcagttatt caggatttta cccgctggca tctgcgttgt tagtgggcgg catttttgca
     1321 gcctctcgtc ggttggatgc gtacggctgg aagttcaaac caattctgcg tccggccact
     1381 ttgctgccgt gtattgtcct ggctgaaatg gtatttttgc tgtcgttaca tccaattcga
     1441 aaaaacggga cccaagagga aacacgtttg cttcgcaacg tgctcgccct caccgaaccg
     1501 acggactatg tcttcgactg caaaggcgaa acggtgtttc gaccacgttg ttttcgaccg
     1561 attttggagg cgatcacgat acgggcgatc cgcagaggaa taatggtgga cgacgcgcct
     1621 gagcgatgca tcggaacaca cacctgcgtt gtcgcgacga tgttaggcaa acgattctcc
     1681 tcctcgacac gccaattcgt gctgcacaac tatctccctg tcgcgaagga cttgcgcgtc
     1741 gcgggaatgg tcttgcacgc gtcacctcaa gatccgcacc gcttgaattt tcaagtcgtc
     1801 gtccccgcgg attacgaaat tatggaggcc gaccggaccg tgtccggcac gcttgacggc
     1861 agcccgtatc agggcgcacg attcctttca gccggcccgc acagcttcga atctgagtca
     1921 ggttcagacg atttaatttt gttgtgggcg aaagccatag atcggcattt cccgtgtctg
     1981 gccacgcgca gctcaccgca tggttaactt cgcgctgcgg catgctcact tgcacatccg
     2041 caacgttacg cgctaactgc ttttagaact ttgcgcggta aaagcgttag cttagcctga
     2101 tccggtggcg cttgcccatc aaattgacaa agatttgcaa gacgcgatgc gcgcaaaaga
     2161 tgcgccgcga ttgagcgttt tgcggatgct aaaagccgcg ctgaaaaatg ccgcaatcga
     2221 aaaatcaggc gcggatgcac aactgagcga ggctgacgcg attcaagtga tccggaaaca
     2281 ggtgagacag cggcaggatt cgatcgagag ttttgaaaag ggcgggcgcg cggaactcgc
     2341 ggcgaaggag cgcgaggagt tgtcgatttt gcaagcgtat ttgccgcagg cgctgagcgc
     2401 ggacgagctg gcgaaagttg tgcgcgaaac gattgccgaa gtcggcgcca cgtcgaaagc
     2461 gcaaatgggc gcggtaatga aagctctgca ggcaaaagtc gccgggcgcg cggatgggaa
     2521 gacgttgagt gcggaagtgc agcggcagtt gtcgtgactc gttgagggat gaagcgttta
     2581 agcgttaaag cggcttgcct gacttcccgc ttcaaccctt caacgcttta acgcttcaac
     2641 gatgctcagc gccatcatcg ttgcggccgg cgccagtgag cggatggggt tcgacaaatt
     2701 attcgcgccg ctcgccgaca aaccggtcgt tgcgcacacg atcgatgctt tcgagcgaac
     2761 aagctgcgtc gacgagatca ttttggtggg acgcaaagac agcttggagg agctacggaa
     2821 ggttcttggt gcgaatacca aagtgcggca tctcgtccct gggggaacgc tgcgccagga
     2881 ttctgtgcga gcgggcttgc aacaggtcga tgcgcgcgcc gattacgtcg ccgtgcatga
     2941 tgcggcgcgt ccgctgatta cgccggaaaa gatcgcgcgc gtgttcggag ttgcgcgcga
     3001 acatggcgcg gccgcgctgg ccgaaccgat cagcgacaca ttgaaacgcg tggatgtcga
     3061 tcttgccgtc agcgaatcgg tcgaccggga tcggcttttt gcaatgcaaa ccccgcaagt
     3121 gtttgcgcgc caactgctga ttgacgcgta cgatgcgctc gcggcggaaa acatttccgt
     3181 aaccgacgaa gtctccgccg tacagcatct cggccggaaa gtcgtcctcg tcccgaatca
     3241 ggatttcaat ttcaagatta cctacccgcg cgattttctg ctcgctgaat tcatttttcg
     3301 ccatagaacg cgcgaagcgg gctagacaaa cattggatca atcgggacga gtggacgcac
     3361 gagttccaca acggcgggaa catgtttggc cttaaatttg cgggaacggc gcaggccctg
     3421 tgaactttcc agttcgctaa cgcgcaatag tttttcattt ttccagaccc aggcgtcggg
     3481 attgtaaggc ccaacgcggc gcgggtcggt ccaggtgtgg atcgaaagca cccgatcggt
     3541 gatcgcggcc gcgatgtgca tcggaccgct gtcgaccgtg atgataaaac tggcggcgcg
     3601 aatcagccag atcagttgca ggagcgaagt ctggttggtc agctcgacgc agttatccgt
     3661 gatgtcgatc ttgcgatttg attttccgac aacgacgacg cgggtcggac tgagcgcgcg
     3721 acaaaactcc tcgattactc gattggagag tgatttaccg ctgcctcggg cgaaggggtg
     3781 gagaagaatg aacggcgggt attgttcgaa gcgcggtagc acgtcgccgc tgggaagtgg
     3841 gcagcgcaac tgatcggtca cggtcgcgcc ggcgcactcc gcgagtttca aatagcgctc
     3901 gatcgcgtgc tcatgccggt tgactttagc ccggcgatga tagaaccagc gtgccccttc
     3961 acgcgcgtcg ctcatgccgt agatttcctt cgcgccgctg attctcgcga tcaacgcgct
     4021 gcggaggagc ccttggaaat cgagcgccag gtcaggtcga agttttcccg tcgtcttaat
     4081 ccagcggccg agactgctcg gcgcacccaa gccgcggaat tcaccgcgcg gaaaaatgtg
     4141 cacatgatcg acatgtggac ttccccggag tagcggcgcg aactcgggat tgatgaccca
     4201 ggtaatttcc gcgtccgaat gcgcatcacg caccaaagcg actgcgggaa gcgtgtggac
     4261 gacatcaccg agcgaactcg gtttaataat caaaatcgat ttcaggtttt cagcgtctca
     4321 actttttcag cgttttgccc tcagcgtccc aacgcgaggc gttcggcgag cagcggggac
     4381 aaacccgact tgatgatttt ttgctgggcg agcgcgagct tgtaactgac gcggcgttgc
     4441 tcgacgaggt ttctgtcggt atcgtaaacg cagtaggcgg cacgccagtc cgcatcgcgc
     4501 ggctggccga cactgccggt gttgatgaaa tatttcttag ttgcatcgat gctgagtcgg
     4561 tcggttttga ggcggctaac tccgccatcg cgaacaaatg ccatcggcac atgtgtgtgg
     4621 ccgaagaaac aaacggtggt gtgttggtag gtgaagctcg ccgccgcgtc gaggatgttg
     4681 aagacgtaac cccattgcga gggcgtgtcg agagtcacat ggacaatcgt gaaatcgcgc
     4741 acctggcgct cgaaacgcaa ttcgcgcagc caatccttgt cgtcttcggt caggttgtca
     4801 cgcgtccatt gcatcgcgtg ctcggccagc gggttgaaat cgcgtgatga ctcgggcagc
     4861 gatgcttgtt cgtcgtgatt acctttgacg atcgggcaat cgagctcgcg cacgcgctcg
     4921 acgcactcgt gcgggttggc gttgtagccg actacatcgc ccaaacaaac gtaatccgtg
     4981 cagctctgtt ggccggcgtc gtccagaacc gcttcgagcg cttcgaggtt ggagtgaatg
     5041 tcgctgaaga cggcgaagcg catgctgttc gtggagtggt taaagcgttg cagcgttaaa
     5101 gcggcggggc aacggcgcac atttaagcat tgaacgcttc aactcttcaa cgcttcaacc
     5161 gattctgatc gggaattcgc tggtccggag gaatgttgag tttgtcttcg agcaacttca
     5221 gccgcgagat caatgtcggc aacctttcct gcggacccat atcgtacagt tggcgcaacc
     5281 gcgcgtaagc ttcttgctcg cggccttcgc aatacgaaag ctcgtaagcc gcaaaacgtt
     5341 tgtcgtagct cggagcgcga ggtaatcccg ccgccttcgc gaaaaactcc gccgcgtgct
     5401 catggtcgtt gtatttgtct ttgtagagac ggccgagcac ttcatagagc tgcggtttgt
     5461 cagggttgtt cgcgattccc cgttcgagga aatctttgcc gagcgcgaaa tattcacgtt
     5521 gcgcctttac gcgcagcgcc agtcggggct gcgttgggtc cttcattgct gccacgctcg
     5581 cattccaagc catgtgccag gcagccatgt cccaaaacaa gatcgagcgc ggctgcaacg
     5641 tcgtgacttg acgaaacagc agaaggaccc ggccccattc cgtctgttcc cacgcaacgt
     5701 gcgcctgaat gaacagcacg tccgcgacgg cggcacgaaa accgctcagc gcggcaacaa
     5761 acccgagctg accgagtttt tcgcgcagat cgagattaaa ttcgacgcca cgcaaccgct
     5821 cttgtcgatg ctgcgccgcg agatcgcgct cgatcggcag tttgattgcc ccgaaaagaa
     5881 gcagcgcaca tgacgtaatg acaaggcgcg tcacaattct ttcccgtaaa acacgaacgc
     5941 ggcgaggaag gtgtagatgg tcgtgtagaa aactccgagc gcagcggttt ttgcaaacag
     6001 cgccgctgga atggccgcgc ctgcgacgac gtcatccacc agattaaacg actgtaagtc
     6061 tggaaaaatc agcgctacaa gcgcgagaaa tatgcgtgtt gtccaggtcg ccgcgtgttc
     6121 ctgcagccag tactcgcgcg cggtcgcctg cagatgccca atgaaataga cgaacaccat
     6181 caccacgatt gtgaagatgc ttgtcgtggc gaatgtcgaa acgaaaaggg tcaacccggc
     6241 gagcaagcac gccttcaaaa aaatcactgt aatccctggc acaagattga cattccaggc
     6301 tgaggcgcgg acaccgcgca aagcgtcctc cagctgttcg tgtggtgccg cgctcatttg
     6361 ccgcgctgtc tcgtgcaaca acacctgttc gcgcgcataa agcaccgcaa gaaacatcgc
     6421 gctcattacg attgtgctga tgaggagcag cagcagcacg ccggcaattt tgccgaggat
     6481 gtattcgata cgcggcaccg gcttggccag gatcgtatag atcgttctgt cctcgacgtc
     6541 ctgcggaata agccgcgccg tcgcgacgat cgccagcaac gaggtgaaga tcgacattgc
     6601 tccgagggag acatccttca ggatttggaa ctcctgttga aacgtcaggc gcgccacgaa
     6661 tattgagctg ccgatgagga gcagcgcgaa aatcagcagc acgtaaaaaa cccgcagccg
     6721 cgtcagttcg gtcagcgtat ttgttgtaat ggcgtaaacc cggcgaaaag aaaacgcgcg
     6781 gtggcgaatc gcgtgcatcg ttagatctcc accgatgtgc tgcctgtctg atttccttga
     6841 gtcgcgtcga gaaacaggcg ctcgagtgtc gttgtcgagg tccgatgggc gagaactctc
     6901 gcgttcgact tggcagccac ggccgtgatc tcgtcgatca gtcgatctga agcgttctca
     6961 agcacgagct cggtttggtt ttcaaccgcg agcagttcat cgagccggcc ttcgcgaacg
     7021 agcaggccgt tcgccagaat gccgacgcga tcgcaaatct cctgcgcctg cgcgagcaga
     7081 tgcgacgaga gcaggacggt gatgccgcgt ccgcgcagac cgacaatcaa attgcgaatg
     7141 tcgcgcgagc cggcgggatc aaccccggcc gtcggctcgt cgagcacgac cagcttcggc
     7201 tcgttgatca gcgcctgcgc cagtccaatc cgttgcaaca tgcctttgga atatgtcccg
     7261 aggcggcggt tccgcgcgtt tgttaagcca accagctcga gcaattcgtc gacacgtttc
     7321 tgcagcgccg cggcgcgtag cccgcaaagc ttcccaaaaa aacgcaaggt ctcttcgccg
     7381 ctgagatatt tgtagaaata cgggttctcc gggagaaaac cgaccgcggc gcgactatcg
     7441 accagggtgc tgtcgcggcc gaaaatctcg gtgcggccgc gagtgggaga aactaagccg
     7501 aggatgatct tcagcgtcgt gcttttgcca gagccgttcg gtcccagtaa accgtaaact
     7561 tcacccggct cgacgcgcag attgaggtcg cacacggcga cgactgattg tcgatgcagc
     7621 gggaccggaa aaactttggt caacccgcga acggcgaccg ccgcacccgc ctctgcgccg
     7681 ctacggcgtg gcaggtcagt catggcagat ttcgaagccg cgcgccgcga ccagctgctc
     7741 caacttttgt tcggtgtgtt tgcggatgtg actccgcaac tcactatcca ttacggcatc
     7801 gaggaatgcg cgcacctccg cgtcttcacc gctcacccgc atctcgacac ggccgtcaag
     7861 cagattgcgc acccagccgg tgacggcgaa gcctttcgcg atgttgcgca ctgtccaccg
     7921 gaacccgacg ccctgcacgt ttccttcaaa gaaaacctga agcgacgtca tcgcttactt
     7981 gtaagaataa acgagacagt aattgctctc ggcgccttca agcagatcga cggcgacaaa
     8041 gtactggccg ctgccggtcg gggaaaaacc ggctgccgct ttcgcgccgg agtcatactt
     8101 ttcggccggc acctgcttgc caccttcatc atagagattg accgtgatct tctttgccgg
     8161 ttcaaccgcg ccgactgaaa accaatactg attcccggcg tagagattga ccgcgatgag
     8221 cgccatgtca tgcggcctga gcaagccgca ccaatggccg tcacgcatct tgaagccatc
     8281 attcgtaaac gcgcctgcca attcgagcgc atttttgcgc gcgtcaactt cgcgatcact
     8341 ttgcgcccaa cccggcgagc cgcacgcaag caagatcgac agtaacaaga tcgacagccg
     8401 caatttcatc tcgcgtcggg tttgccttga atgctttcaa tggtccggcc gaccgcttcg
     8461 ttgactttcc ggacctcatc gggagttggt gctttgccgg gcgcaaatga aacgagcttt
     8521 tcgatactaa ctaattggtc gtcgacgtgc ttcactaacg gttcgtcgcg cagctccggc
     8581 gaaatgccgt tcactttcga atggatgaaa ctcacgagtg ccggctgccg cagcactttc
     8641 gccgcgcgct catcgtaatt ttccatgatg gccgacgtaa ccacctgcgt accgcgcagc
     8701 cagccgccaa cgctgacgag aataatcagg tcctgatcgc tgtgcgtttg catcgaggat
     8761 ttgacttcgt tttgcgtcgc ttcgagttct tcctgaagcg cgtcccattc gtcgttctcg
     8821 gcaaaatcgt tcaggctatt gccgcgccgg agcaggttct cgctaacgcc gagtgctttc
     8881 gagagcttga tgatgtcgga accaatgttc ttgacctgct ggctgtcttt cgcttccacg
     8941 gcgacgaaac cgtcggcgat taacccaccg aggttgagcg caatctgcgc gcggttgcga
     9001 taggtcatcg gaatcggctc acggtattga cctgaccagt tcggcttgcc cggcttttcc
     9061 agcgcggcga aaagttcgcc cggtgtcgga atgctgatct cctcggcttt aatcgctctg
     9121 gcgagtctat cgggcgaaag acgggctggc tcggcgccga tgacgcagta acaagcgatc
     9181 agcgttgcaa ttgcaacagc ccgcatgagc cgtttcaccc gccaccctag ccgccgacac
     9241 gcggaaatgc cagcgcgaat cttcagtgca gaagttgatt gtcaccgccg cggaactgca
     9301 ctaatcgagt cgctgcatgg gaaaaacgac catcgctgaa gttcgcgctc gcgagattct
     9361 cgattcccgc ggcaatccca ccgtcgaagt cgatgtgaga ctggagagtg gcgggcttgg
     9421 tcgcgcagca gttccgagcg gcgcgagcac gggcgaacac gaagcatggg agctgcgcga
     9481 tggcgacaaa cgacgttatg gcggcaaagg cgtgtcacac gcggtcgcga gcgtgaatga
     9541 aaaaatcgcg cgtgagatca gaggtttgga cgcgcgtgac caggcgaaga tcgacaatcg
     9601 cttgattgag ctggacggta cgcctaacaa gaacaacctc ggggcaaacg cgctgctcgg
     9661 cgtctcactt gccgtggcgc acgctgcagc ggccgcggaa aatctaccac tgttccgata
     9721 tttaggcggt ggtgaggcac gagttctacc agtgccgatg atgaacattt tgaacggcgg
     9781 ggtgcattcc gatgcgccaa tcgattttca ggaattcatg atcgtgccgc gcggcgcgcc
     9841 gacttttgcc gaagcgctcc gttacggcgc ggaggtgttc cacaccctta aacgcgtgct
     9901 gcaggatcga catctctcca ccgcgattgg agacgaaggt ggttttgccc cgcaattgaa
     9961 ctcggccgaa gacgcgctcg agacaatcgc agcggcggtc gagaaggccg gttattcgtt
    10021 aggcaaccaa gtcttcattg cgctcgaccc ggcgtcgtcg gagttcttcg acgccgagcg
    10081 cgggtgttac gtgttcaaaa aatcgggcgg cgcggaaaaa tctgtcgaag aactgattca
    10141 gtactatgtc gatctttgcc gccggttccc gatcatatcg atcgaggatg gctgcgcgga
    10201 gaacgattgg gacgggtgga aaaaactaac ggcgcgactc ggccaccaga tccagctcgt
    10261 cggcgacgat cttttcgtca ccaatgtgaa attccttcgc agaggaatcg aagagcacgt
    10321 cgcgaattcg attctgatca aagtaaatca gatcggcacg ctgactgaga cattagcgac
    10381 gatcgaactc gcgaaaaaga acaattacac cgccgtgatc agccaccgct ctggcgaaac
    10441 cgaagacacg accattgccg atcttgccgt ggcgacaaat gccggccaaa tcaaaaccgg
    10501 ttcgctttgc cgcagtgatc gggtcgcgaa gtacaatcag ttactccgca tcgaggaaga
    10561 acttggtgac aaggccgttt acggcggtaa gctgcgctga ggtggagcag agttacggcg
    10621 atttccgagc gcgtcgcgaa gccaccgttt ggcagcgcct taatcgcgtc cttgcggtcc
    10681 tgctcgttct cgccgtctgg ctcgttatca tcagtctgtt tgtcccgcct tacaaaaggc
    10741 tgatgcagag ccgcgcggag attgataatc tgcaaacccg cgttgatgag cagaaaaaac
    10801 tgctcggccg ccgaagccac gaagtcactc tgctacaaac cgatgcgacg tacctggaaa
    10861 caatcgcgcg cgatcgcctc gacctgatga aggaagggga aaccattttc cggctcgaac
    10921 cgacgcagcc tgggaaaggc agacctcccg cgcggcctta gcttttgtcc aacagcgcgc
    10981 cgctagcctg ctgtcattcc gagcgcagcc gaggaatctc ttactgttaa agcaacaaag
    11041 cgatgtctcg gcttggctcg acatgacaag ttcgcgcgcc acgatatctt aggtgccatg
    11101 tctaacgagc ttgcgacagt tgatgccgaa gacttaaagt ctcgcctcgg cgagttgcgg
    11161 aggtttcttt gacctgccca ggctccagca gaatctaacc gcgctcgagg cgcggatgag
    11221 cgagaaaaat ttctgggaca atcgcgagcg cgcacaggct gatgtcgagg aactctcgcg
    11281 tctgcgctcg ctcattaatc catttcacga gctcgagcgc gagatcagcg atttcgaggc
    11341 gctgcagcaa ctcgcgaccg aggagcagga cgaatcgcat cgcgtccaag ccgagcgcga
    11401 gcttgcggcc gagcacgaac gtttgctgca taagctcaat gaattcgagc tgcgccagtt
    11461 tctctccggc gaaaacgatc gggcgaacgc gtttctcacc atccactccg gcgcgggcgg
    11521 caccgagtcg tgcgattggg ccgacatgtt gttgcgcatg tatcaacggt ggatcgagcg
    11581 caacggtttc aaatcacagg ttatcgatgt tcaaaccggc gaagaagtgg gtatcaaatc
    11641 ggtgacgctg cttgtcaccg gcgactacgc ttacggctac ctccaaaccg agcgcggcgt
    11701 gcaccggctc gtacgcatct cgcccttcga cgcgaacaag cggcgccaca cttcatttgc
    11761 cagtgtcgat gttgttcctg aaatcgcgga cacggcgccg atcgaaatca atctggccga
    11821 tctcgagatc gacaccttcc ggagcggcgg caaaggcggc cagaacgtga acaaagttga
    11881 aacggctgtg cgcatcgtgc acaaaccgac aggtatcgtg gtcgcctgcc aggccgagcg
    11941 cagccaggga cgcaatcgcg acctcgcgct gaaaatgttg aaggcgaaac tttaccagat
    12001 cgaggaggac aaaaagcgcg ccgaaataga tcgacaatat ggcgagaaag gcgagatcgc
    12061 gtggggcaat cagatccgct cgtacgtttt ccagccctat caaatggtga aagaccaccg
    12121 caccggcgcc gagaccagca acgtgcagga ggtaatggac ggcaacatcg ggatgtttat
    12181 ccaggcgaaa ttgcgcggcg agaaaggcaa agagtagcgc aagcctttgg cttgcgaacg
    12241 tgagaatcgc aagcgggacg cttacgctac gttttcacga gctgcagtcg aaaccgagga
    12301 tcgaactcgc cgtgattgta ggcgcgacag catcgcaggc aggcgaccgt gcgacgaatg
    12361 cgccgcaccc gcggaaactc acggcggcag ttcggacagc gatagatgaa ccgcgtcgga
    12421 aaagtgcgcg cggtaaacgg caggttgtga caacgtgctt catcggcaat gcccaaatcg
    12481 acacacgcgt cgcgccactc gggcccgtgc ggcagaatcc gacggcgccc ggcgcgaaac
    12541 tgggcgagca aatgcgccag c
//
LOCUS       DASTDG010000151         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_480649, whole genome shotgun sequence.
ACCESSION   DASTDG010000151 DASTDG010000000
VERSION     DASTDG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480649"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFO30_04975"
     CDS             complement(<1..664)
                     /locus_tag="VFO30_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418673.1"
                     /translation="MAISESGRAARYEQRRLARLLVSTLILLGACSGGIKQPIEQASD
                     VSYVVDPSVNFSVTSQHGTICIYGSDSPELRVHTVKKAYSVARLEKLDQKIVVRADAV
                     SIRTEFPPPRTWGWADRSGTVDYSIVLPQTATITRLELENGEVMVEGIREGAVHARLG
                     SGLMFVRNCFSDTDVAVTTGNLTLSYDWWEQIAFTATATVRKGNVWMFIPGEAAFHLS
                     AQA"
     gene            complement(651..947)
                     /locus_tag="VFO30_04980"
     CDS             complement(651..947)
                     /locus_tag="VFO30_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418674.1"
                     /translation="MKPIPSFSDYHAGMARRSRRFPSIDCHYQTPPHVDLGGGHCAPH
                     SSFRSISRDYFRDEAPYHFAAEAALFAAMIVTSGIAVAIGTVAAIDFLRALGYI"
     gene            1109..2347
                     /locus_tag="VFO30_04985"
     CDS             1109..2347
                     /locus_tag="VFO30_04985"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9418675.1"
                     /translation="MHKVARQRPDWETIREDFPVLRQNVHGEQLIYFDNAATTQKPRP
                     VLERLRHYYEHDNANVHRGLHELSSRATAAYEAARQRVADYIGAATAEEIVFTRGTTE
                     AINLVAQAWGGKFIRPGAVILLTEMEHHSNLVPWQLLAERSGARLRFVPVNKDGTLAL
                     DQVASLLTREVKLFAFTHVSNSLGTINPVAELCERAHAAGALTLIDGAQSAGHMPVDV
                     QEFGCDFYAFSGHKMCAPTGIGALYARAEILDALPPWHGGGEMIVSVALEKSVFKKAP
                     HRFEAGTPNIAGAIGLAAAIDYIDNIGRVAIFEHDAELATHAVEQLADLPGMRVLGPS
                     GQRGAIVGFVMDSVHPHDLTTFADQRGLALRGGHHCNQPLMRKFNLPGTARASFYFYN
                     TKEEVDRMIDILRDAVRFFS"
     gene            2344..>2663
                     /locus_tag="VFO30_04990"
     CDS             2344..>2663
                     /locus_tag="VFO30_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9418676.1"
                     /translation="MSIELEELYQEIILDHSRRPRNFGELPNAAVVVHGDNPACGDEI
                     HLAVRFGDDGSLQDIKFTGHGCAISQASASMMTTKLKGKSRDETMSILHKFRDLVTGD
                     GETPP"
BASE COUNT          573 a          817 c          769 g          504 t
ORIGIN      
        1 gcgcctgagc agaaaggtgg aaggcggctt cgccgggaat aaacatccag acatttcctt
       61 tgcgcacagt cgcagtggcc gtgaacgcaa tctgttccca ccagtcatag ctcaatgtca
      121 gattaccggt cgtcaccgcg acatccgtgt cgctgaagca attgcgcacg aacatgagcc
      181 cgctgcccag ccgcgcatgc accgcgccct ctcgaattcc ttcaaccatc acttcaccgt
      241 tctcgagctc gagacgcgtg atcgtcgccg tctgcggcag aacgatcgag taatcgactg
      301 tgccagaccg atcagcccag ccccacgttc gcggtggcgg aaattcagtc cttatcgata
      361 ctgcatcggc tcggaccaca attttctgat caagtttctc cagccgcgcc accgaatacg
      421 ctttcttgac cgtgtgcact cgtagctccg gcgagtccga gccataaatg caaatcgttc
      481 cgtgctggct cgtgacgctg aagttcaccg agggatcgac gacgtaggaa acatcgctcg
      541 cctgttcgat cggctgctta atccctccac tgcaagcgcc gagcagaatc agcgtcgaga
      601 caagcagccg tgccagccgt cgttgctcat accgagcggc gcgcccgctc tcagatatag
      661 ccaagagccc ggagaaaatc gatggcggcg acggtgccga tcgcgacggc aatgcccgaa
      721 gtgacaatca tcgcggcaaa aagagccgcc tcggcggcga agtggtaagg cgcttcgtcc
      781 cgaaaatagt cgcggctgat gctccgaaac gaggaatgcg gcgcgcaatg gccgccacca
      841 aggtcgacat gtggcggcgt ctgataatga caatcgatag acgggaaacg ccgcgagcgt
      901 cgagccatgc cggcgtggta atcgctaaag ctggggatcg gtttcataag gtctttgtct
      961 ctcgccagtg ggatgcgttc aatcccgtcg ccggattcaa agcttcatcg gaaatccact
     1021 ttgcacacca gcaactccta tgcaagctgc ccgccgcctt gggatgaaaa gagtggcgcg
     1081 ttacgccacg cagcgcgaag tttgacacgt gcacaaagtc gcgcggcagc gcccagattg
     1141 ggagacgatc cgcgaggatt ttcccgttct tcgccagaac gtccatggcg agcaattgat
     1201 ttatttcgac aacgccgcga cgacgcaaaa gccgcgaccc gtcctcgaga gactgcggca
     1261 ctattacgag cacgacaacg ccaatgttca tcgtggcctc cacgagttga gctcgcgcgc
     1321 gacggccgct tacgaagcgg cgcggcaacg ggtcgctgat tacatcggcg cagccactgc
     1381 cgaggaaatc gtcttcaccc gcggtaccac cgaggccatt aacctggtgg cgcaagcctg
     1441 gggcggaaag ttcattcggc cgggcgccgt gatcctgctc actgaaatgg agcaccacag
     1501 caacctcgtg ccgtggcaat tgctggccga gcgcagtggc gcgcgcttgc gttttgttcc
     1561 ggtgaacaaa gacggcacgc tcgcgctcga tcaggttgcg tcactactca cgcgagaagt
     1621 gaagctgttc gccttcacgc atgtttcaaa ttcgttaggc acgatcaatc ccgtagctga
     1681 gctttgcgag cgagcccatg ccgccggcgc cttgacgctg attgatggcg cgcagagcgc
     1741 cggccatatg cccgtcgacg tccaggaatt cggctgcgac ttctatgcat tttccggtca
     1801 caaaatgtgc gcgcccaccg gcattggggc gctctacgcc cgcgccgaaa tcctggatgc
     1861 gcttccgccg tggcacggcg gcggcgaaat gatcgtgagc gtggcgctgg aaaagagcgt
     1921 cttcaaaaaa gcgccacatc ggtttgaagc cggcacgccg aacattgctg gcgcgatcgg
     1981 cctggcggcg gcgatcgatt acatcgacaa catcggccgg gtcgccattt tcgagcacga
     2041 tgcggaactc gcgacccatg ctgttgagca attggccgac ttgcccggca tgcgcgtgct
     2101 tggaccgtct ggccagcgcg gagcgatcgt cggcttcgtc atggattccg tgcatccgca
     2161 cgacctcaca acatttgccg atcagcgcgg gctcgccttg cgcggcggcc atcattgcaa
     2221 ccagccgttg atgcgaaagt tcaatctgcc gggcaccgca cgcgcgagtt tctatttcta
     2281 taacacgaaa gaagaagtcg atcgcatgat cgacatcctg cgcgacgcgg tgcgtttctt
     2341 ctcatgagca tcgaactcga agagctgtat caggaaatca tcctcgatca ttcccgacga
     2401 ccgcggaatt tcggcgaact accgaatgcg gcggtggtcg tgcacggcga caacccggcg
     2461 tgcggcgacg aaattcatct ggcggttcgc ttcggggacg acggctcact gcaagacatc
     2521 aaatttaccg gccacggctg cgccatcagc caagcatcgg catcaatgat gaccacgaag
     2581 ctcaagggaa aaagccgaga tgagacgatg tcgatcttgc acaagtttcg cgatctggtg
     2641 accggcgacg gcgagacacc acc
//
LOCUS       DASTDG010000152         9663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1224642, whole genome shotgun sequence.
ACCESSION   DASTDG010000152 DASTDG010000000
VERSION     DASTDG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224642"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /gene="recR"
                     /locus_tag="VFO30_04995"
     CDS             <1..505
                     /gene="recR"
                     /locus_tag="VFO30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET9418677.1"
                     /translation="IQARADAPEQIARAINETRRAIQPCKRCGYFTSDEICDICADAS
                     RAADILCVVEQPTDILPLEKTGAFGGRYHSLGGRISPLDHVGPEDLRIKELLDRIDAE
                     KFSEIIFALAADVEGEATTNYIVDLLKDKPLTLTRIARGIPAGGGLESADELTLSAAL
                     SGRTKVR"
     gene            588..1475
                     /gene="lipA"
                     /locus_tag="VFO30_05000"
     CDS             588..1475
                     /gene="lipA"
                     /locus_tag="VFO30_05000"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9418678.1"
                     /translation="MSCAKIDPALHQPNKPPWIKVRLPSNPVFFSTKALISDLRLHTV
                     CESAQCPNRWECWSAGTATFMIAGDRCTRACGFCAVTTARPFALEEDEPQRVAEAVQR
                     MKLKHVVITAVARDDLKDGGANHFARVITAIRAMDPSIILEVLVPDFHAQDWCIQAVL
                     DAAPDIYNHNLETVERLTPFVRSRAKYRTSLRVLQRAKELSPRIVTKSGVMLGLGETE
                     PELFQTMDDLRDVGCQVLTMGQYLRPTPNHLPVVEYITPEQFELYGQIARKKGFEHVA
                     SGPLVRSSYHAADFHPVVR"
     gene            1478..2203
                     /locus_tag="VFO30_05005"
     CDS             1478..2203
                     /locus_tag="VFO30_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9418679.1"
                     /translation="MEHPGAARSTIAAVIPAFNEEKHIADVVRRTRAKIDNVLVVDDG
                     SGDATAERAREAGADVFVHPDNRGKGEAIRTGLRHWLDRHFEWIFILDADAQHRPEEI
                     DRFVDATSSSSEPRLILGNRMNDVSSMPLVRRIVNRYMSKRISRVCGQQIPDTQCGFR
                     MLHRQLIPHLLNGADRFDYETEMLIVASRKGFRIDSVAISTVYSDEISSIHPVRDTLR
                     FVQLMRRYKNRGPDSGGGAAAVR"
     gene            complement(2196..2717)
                     /locus_tag="VFO30_05010"
     CDS             complement(2196..2717)
                     /locus_tag="VFO30_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418680.1"
                     /translation="MKEDDFHYAIENTQVIVAPEQQIATFGSTSFHFYLISELMDRVD
                     QVRVRNGKIQAERPQILTPEHFRRLLLEGFGEKAQRYAEQLRENLRDIAVLRYGFQFR
                     KTDVTEETIRDSLNAVITRTKRRVENADQPLSAIIHGVDDAWEVCLLKFTIDMVERSA
                     GGNLGDLRKRGLI"
     gene            complement(2801..3448)
                     /gene="msrB"
                     /locus_tag="VFO30_05015"
     CDS             complement(2801..3448)
                     /gene="msrB"
                     /locus_tag="VFO30_05015"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9418681.1"
                     /translation="MSELSIQEMTKPPARRIHHPSKWTTHLTLWLGLLGALAVIAGFF
                     FLGHKQEVQKRAGTFNYLKPVPSESQLRVQLTEQQYRVTRQGATETAFRNEYWDNFKP
                     GIYVDVITGEPLFSSTDKFDAGTGRPCFTKPISSERLYETIDTSAGMQRVEVRAKRSN
                     SHLGRVFNDGPPPTGKRYAIESAALRFVPVEQMQAKGYAEFLSLFPESQRPTATP"
     gene            complement(3570..4220)
                     /gene="msrB"
                     /locus_tag="VFO30_05020"
     CDS             complement(3570..4220)
                     /gene="msrB"
                     /locus_tag="VFO30_05020"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9418682.1"
                     /translation="MTRHRVFLHVIQFPQRPKPPSRGMLIFCAFVFVGLLGLLIFLNH
                     AGAQRKDEPASQTPAMMNPASSSEKSGQASDADLRKRLTPEQYHVTQMCGTEPPFQNA
                     YWNEHRAGIYVDVVSGEPLFSSLDKFDSGTGWPSFAKPIDKARVAEKSDRTLGVERTE
                     VRSTKSDSHLGHVFPDGPAPTGQRFCINSAALRFIPVEKLKQEGYGDYLALFEKKK"
     gene            complement(4447..4893)
                     /gene="tnpA"
                     /locus_tag="VFO30_05025"
     CDS             complement(4447..4893)
                     /gene="tnpA"
                     /locus_tag="VFO30_05025"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET9418683.1"
                     /translation="MHSFNSCVIHFVWSTKNREHTLTAELRERLWPYLGGIAKQNKMR
                     ALAIGGAADHVHMLVSLPPTLSVAKAIQLLKGNSSKWIHETFAHMRSFEWQEGYAAFS
                     IGISGIDATVAYIRNQAEHHRTKSFREEFQAMLRKHGSDYDARMTD"
     gene            complement(5050..6534)
                     /locus_tag="VFO30_05030"
     CDS             complement(5050..6534)
                     /locus_tag="VFO30_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9418684.1"
                     /translation="MAEATKALPAEEIVTPIAPAGVHGFIAKFTVLRGAQRELWLTFL
                     IKFLIYTAYSITNKTMVLWLSKDLGFSDQAAGALVGWVWAPAMTVFTLLAGSLTDAIG
                     LRRTFFLGVAICTVARSVMVLTTNPSLALACGVLPLAVGEALGTPVLLAATRRYSTTA
                     QRSMSFSVIYMVMNVGYIAAGWIFDYIRQLDLRFSLFGFEPTTHQQLFLVSLGFELVL
                     FPVIYLLRRDSKAAIPTEARRSVPGFWSRIGQTVRQSAEETAQLFRKLLGQSGFYRLL
                     AFFLFIGFLKAIFLQMDYVFPKFGDRELGLHAPVGKIAAINSILIIVLVPIVGALTQR
                     FAAYRMVVIGGIICAAGVFVMALPTEWFQAAANGPIGQWLGHTYLGVRGSIHPYYVMA
                     ALYLAVFSVGEAFYSPRVYEYAAAIAPHGQEASYGALAYIPFLIGKLLVGTAGWILAA
                     FCPERGPRHSGIMWLIFALAASVAPFGLIVFRRYIRVPEAGRQD"
     gene            complement(6550..7398)
                     /gene="ftsY"
                     /locus_tag="VFO30_05035"
     CDS             complement(6550..7398)
                     /gene="ftsY"
                     /locus_tag="VFO30_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9418685.1"
                     /translation="MKFLETLAQTFANKPIDWDELEHALIRADLGVPLTMRIIERAKE
                     HEAWSLLGVSNVIEVAREEILKVLPKRAEPLRAARGRPAVVLILGVNGTGKTTSTAKL
                     AHFLLEHRHSVLLAAADTFRAAAIEQLGIWAERLGVEMVRSQYGADPAALCYDAYQKA
                     AKQKVDFLLCDTAGRLHTKSNLMAELQKVKRTLAKLDSDAPHETLLVIDATTGGNALN
                     QAREFNQALGLSGLIVTKLDGSGEGGIVVAIQAELGMPTRFIGTGEKIDNFAPFDARE
                     FVANML"
     gene            complement(7492..7941)
                     /gene="nusB"
                     /locus_tag="VFO30_05040"
     CDS             complement(7492..7941)
                     /gene="nusB"
                     /locus_tag="VFO30_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9418686.1"
                     /translation="MGKRRRAREAAIQYHFWRDLQHGAGPEKLEDFWDFCPVKPSVRE
                     FAQPLIEGMGEHLPEIDERIGKYAENYNLNRISTVDRNVLRLAIYEMLYRDDIPPVVS
                     INEAIELAKTFGGAESGKFVNGILDRVRKELNRPAREPLVRQGPPQQ"
     gene            complement(7934..8419)
                     /gene="ribH"
                     /locus_tag="VFO30_05045"
     CDS             complement(7934..8419)
                     /gene="ribH"
                     /locus_tag="VFO30_05045"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9418687.1"
                     /translation="MSKTAPRPARRSARPHKFAVVASRFNSYYVDAMVCHATEELRAL
                     APGANISVQRVPGAFEIPVVVRELASRNKADAIIACGVILKGETNHAQNLSRSVTDAL
                     QRIAVDRGVPVINAVLSFDNEMQARERCLGEKINRGTEAAGAAVEIARTISNLRRKKH
                     G"
     gene            complement(8464..>9663)
                     /locus_tag="VFO30_05050"
     CDS             complement(8464..>9663)
                     /locus_tag="VFO30_05050"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET9418688.1"
                     /translation="QTFQFDAIDDVVSDIAKGRIVIVTDDADRENEGDLVMAAEKVTP
                     EAVNFMATHGRGLICVPISNDRAEQLGLQRMVAQNREAQRTDFTVSVDAARGVTTGIS
                     AYDRATTILAIANAHSTPDDLAQPGHVFPLRAKDGGVLRRAGHTEAAVDLAQMAGLQS
                     AGVLCEILHDDGTMARLSELMQFRKKHGLRICTIQSLIAHRRKREKLVEREQVVKLPT
                     DYGDFELHLYRSKLDGMHHLALVKGKIDKNKPTLVRVHSECLTGDVFGSKRCDCGNQL
                     HAALRQISEEGNGVLVYMRQEGRGIGLAAKIHAYKLQEEGLDTVEANAKLGYPSDLRD
                     YGLGAQILFDLGVRQFRFLTNNPKKVVGLEGYGLEMIEQVPIRAEANPHNVRYLETKK
                     VKLGHAL"
BASE COUNT         1975 a         2855 c         2781 g         2052 t
ORIGIN      
        1 gattcaggcg cgcgctgacg cgcccgagca aatcgcgcgc gcgatcaacg agacccgtcg
       61 cgccattcag ccgtgcaaac gctgcggcta cttcacctcc gatgagatct gcgacatctg
      121 cgccgatgcg tcgcgtgcgg ccgacattct ctgcgtcgtg gaacagccca ccgacattct
      181 gccgctcgag aaaaccggtg cgttcggggg acgttaccac tcgttaggcg gccgaatttc
      241 tccgctcgat cacgtcggcc cggaggattt gcgcattaag gaactgctcg accggatcga
      301 cgccgaaaaa ttctccgaaa ttatttttgc gctcgccgcc gacgtggaag gcgaggcgac
      361 gaccaattac attgtcgatc ttttgaaaga caaaccgctc actttgacac gaatcgcccg
      421 tggcattccc gctggcggtg gcctcgaatc ggccgacgag ctcacactgt ccgccgcgct
      481 ctcagggcgg acgaaggtcc ggtgaagcgt gaagagttga agcgataggg cgccgcttta
      541 acgcttcaac ttttcaacgc ttcaacgctg cgctaatctt tagtgcaatg tcctgcgcta
      601 aaatcgatcc ggcgctccac cagccaaata agccgccatg gatcaaagtg cgtctgcctt
      661 cgaaccccgt tttcttttcg accaaggcgc tcatttccga tctgcggctg cacaccgtct
      721 gcgaaagcgc gcaatgtccg aatcgctggg aatgctggag tgccgggacg gcgacgttca
      781 tgatcgcggg ggaccgctgc acgcgcgcgt gcgggttctg cgccgtcacg acggccaggc
      841 ctttcgcgct tgaagaagat gaaccgcagc gggtggccga agcggtgcag cgcatgaagc
      901 taaagcacgt cgtcattacg gccgtcgcgc gcgacgattt gaaggacggc ggcgcaaatc
      961 atttcgcccg tgtgatcacc gcgattcggg cgatggatcc gtcgatcatt ctcgaagtgc
     1021 ttgtgcccga tttccacgcc caagactggt gcatccaggc cgtgcttgac gccgcgccgg
     1081 acatttacaa tcacaatttg gaaacagtcg agcggctgac gccatttgtt aggtcgcgcg
     1141 ccaagtaccg gacgtcgctc cgggttttgc agcgcgcaaa ggagttgtcg ccgcggatcg
     1201 tgacgaaaag cggcgtcatg ctcgggttgg gagaaacgga gccggagcta ttccaaacga
     1261 tggacgacct gcgcgatgtc ggttgccagg tgttaactat gggtcagtat ctgcgcccga
     1321 cgccgaatca tctgcccgtg gtagagtaca tcacgcccga gcagtttgag ctgtatggcc
     1381 agatcgcgcg caagaaaggt ttcgagcatg tcgcgtcggg cccgctcgtc cgcagctcct
     1441 accacgccgc cgattttcat ccggtcgtcc gctgacgatg gagcaccccg gcgcggcaag
     1501 atcgacaatc gccgcagtca tcccggcgtt taacgaagag aagcacatcg cggacgtcgt
     1561 ccggcgcacg cgtgcgaaga tcgacaatgt gctggtggtc gacgatggct cgggcgacgc
     1621 cacggcggag cgcgcacggg aagcgggcgc agatgtgttc gtgcatccgg acaaccgcgg
     1681 taaaggcgaa gcaattcgca ccgggctgcg ccattggttg gatcgacact tcgagtggat
     1741 cttcattctc gatgcggacg cgcaacatcg cccggaagag attgaccggt tcgttgacgc
     1801 gaccagctcg tcgtcagaac cacggctcat cctcggcaat cgcatgaacg acgtgagctc
     1861 gatgccactc gttaggcgaa ttgtgaatcg ctacatgagc aagagaatca gtcgcgtctg
     1921 cggtcaacaa attcccgaca cacagtgcgg cttccgcatg ttacatcgac aattgatccc
     1981 gcatctgctc aacggcgcag accggttcga ttacgagacg gaaatgctca tcgtcgccag
     2041 ccgcaaaggt tttcgcatcg actccgtcgc gattagtacg gtttactccg acgaaatcag
     2101 cagcattcac ccggtgcgcg atacgctccg cttcgtccaa ctaatgcggc ggtacaaaaa
     2161 tcgcgggccg gacagcggtg gaggtgccgc cgcggttaga tgagcccgcg cttgcgcaag
     2221 tcgccaaggt tgccgccggc ggagcgttcg accatgtcga tggtgaactt gaggaggcaa
     2281 acttcccacg cgtcgtccac gccgtggata atggcgctga gcggttgatc ggcattttcg
     2341 acgcgacgtt ttgtgcgagt gatcacggcg ttgagcgaat cgcggatcgt ttcctcggtc
     2401 acatcggttt ttcggaattg aaacccgtag cgtaacacgg caatgtcccg caggttttcg
     2461 cgcaattgtt cagcgtagcg ttgcgccttt tcgccgaagc cttcgagcaa caaacgccgg
     2521 aaatgctccg gcgtgaggat ctgcgggcgt tcggcctgta tttttccgtt gcgcacccgg
     2581 acctgatcga cgcggtccat caattcggag atgagataaa aatgaaagct ggtgctgccg
     2641 aaggtcgcga tttgttgctc gggcgcaacg atcacctgcg tgttttcgat cgcgtaatga
     2701 aagtcgtcct ccttcatgct cgcctaacga aacatagaag atcggactct tcgcggcaaa
     2761 tccgtagcgg cggtgtctga ccgccgaaac ctgcccgcta ttacggcgtc gccgtgggcc
     2821 gttgtgattc tggaaacagg gagagaaatt cggcgtaacc tttcgcctgc atttgttcga
     2881 cgggaacgaa tcgcaacgcc gccgattcaa tggcgtagcg tttgccggtc ggtggcggcc
     2941 catcgttgaa aacgcgaccg agatgcgagt tgctccgttt ggcacggact tcgacgcgct
     3001 gcataccggc cgacgtgtca attgtttcat aaaggcgctc ggacgaaatc ggtttggtaa
     3061 aacacggtcg gccggtgccg gcgtcgaact tgtcggtcga actgaagagc ggttcgccgg
     3121 tgataacatc cacgtaaatc cccggcttga agttgtccca atactcgttg cggaacgccg
     3181 tctcggtggc gccctggcgg gtgacgcggt actgctgctc ggtcagttgc acgcgcaact
     3241 gcgactcgct cggcaccggt ttgaggtaat tgaaagtgcc ggcgcgcttt tgcacttcct
     3301 gtttgtggcc gagaaagaag aaacctgcga tgacagccag cgctccgagc aggccaagcc
     3361 acagagtcag gtgcgtcgtc cacttcgagg ggtgatggat tctgcgggcg ggcggtttgg
     3421 tcatttcctg aatggataat tcactcattg gccggtcagt tcgtctggcg aatgcgcagg
     3481 cgatgttaga gggacttctc tagtatatcg cagcggcgct gcccgctggt tgggcggcgc
     3541 atgcatctct cccacccttg aactgacttt tacttcttct tctcgaacag ggcgaggtaa
     3601 tcgccgtagc cttcttgttt caatttctcg accggaatga accgcagcgc ggccgaattg
     3661 atgcaaaagc gctggccggt cggcgcaggt ccgtccggga aaacgtgtcc cagatgcgaa
     3721 tcacttttcg ttgagcgaac ttctgtccgt tccacgccga gcgtgcgatc cgatttctcc
     3781 gccacgcgcg ctttgtcgat tggcttggcg aaactcggcc agcccgtgcc gctgtcgaac
     3841 ttgtcgagcg aactgaagag cggctcaccg ctcacaacat cgacatatat gccggcgcgg
     3901 tgctcgttcc aatatgcgtt ctgaaacggc ggctcggtgc cgcacatctg ggtgacgtga
     3961 tattgctcgg gcgtgagtct tttgcgcaga tctgcgtctg acgcttggcc gctcttttcg
     4021 ctgctgctcg ctgggttcat catggctggt gtctggctgg ccggttcatc ctttctctgt
     4081 gcgccggcat ggttgaggaa gatcaaaaga ccgagcaatc ccacaaaaac aaaggcgcag
     4141 aagatcaaca ttccgcgcga gggtggcttc ggccgttggg gaaactggat cacgtgcaaa
     4201 aatacgcgat gtcgtgtcat cgcaacacgg aaatttctcc gcaatcaacc acgaatggac
     4261 acgaatggac acgaattaca ggacaatgcg ttcccattcg agcttgggat gtttgcagtt
     4321 gaggatcact ccgacgcgta acttgatgat gcgcacgtaa acagggcgga gtcggtaata
     4381 gcccagcgtt ttaacgctgg gccctaggta ttcgccaggg tcagagtccc aacagggacg
     4441 aaagaatcaa tcagtcatcc gcgcgtcgta gtccgagcca tgcttgcgca gcatggcctg
     4501 aaattcttcg cgaaacgatt tggtccgatg atgctcagcc tggtttcgga tgtaggcgac
     4561 agtggcgtcg attccggaaa taccgatgct gaacgccgcg tatccttcct gccattcgaa
     4621 cgaccgcata tgcgcaaatg tttcgtgaat ccatttcgaa gaatttcctt tgaggagctg
     4681 aatcgccttt gccaccgata atgtcggggg caaggaaaca agcatgtgca cgtgatcagc
     4741 agcacctccg atcgcaagtg ccctcatttt gttttgtttc gcaattccgc caaggtacgg
     4801 ccacagcctt tcgcgcagtt ccgcagtcaa agtgtgctct cgatttttcg tgctccacac
     4861 gaagtgaatc acgcagctgt tgaatgaatg catgcgtctg ccgtccctgc cgggactcga
     4921 cgtgctatat catgactgaa cccagcgtta aaacgctggg ctactctcga tcgcgcatcc
     4981 gacgatctga aacacctcct gtttgtacga gagtttctct gattcgtgtg cattcgtgtc
     5041 catttgtggt taatcctgcc gaccggcttc gggcacacgg atgtagcgcc ggaacacgat
     5101 caagccgaac ggcgcgaccg acgccgccag cgcaaagatc aaccacatga ttccggaatg
     5161 ccgcggcccg cgttcgggac aaaacgccgc gaggatccat cccgccgttc cgacgagaag
     5221 cttgccaatg aggaatggaa tataggcgag cgctccgtaa gacgcttcct gcccatgcgg
     5281 tgcgatggca gcggcgtact cgtaaactcg cggcgagtag aaagcttcac cgacggagaa
     5341 cacggccaaa tagagcgctg ccatcacgta gtatggatga atacttccgc ggactcccag
     5401 gtaagtgtgc ccaagccatt gcccaatcgg gccatttgcc gccgcttgaa accattctgt
     5461 aggcagcgcc atcacgaaaa ctccagccgc acaaataatc ccgccgatga cgaccatgcg
     5521 ataggcggcg aaccgttgcg tcagcgcgcc gactatcggc accagcacga tgatcaggat
     5581 cgaattgatc gcagcaattt taccgaccgg cgcgtgcagc ccgagctcac gatcgccaaa
     5641 tttcggaaac acgtaatcca tctggagaaa gatggctttg aggaaaccga tgaagagaaa
     5701 gaaagccaga aggcgataga aaccagactg gccgagcaat ttccgaaata gctgcgcggt
     5761 ctcctcggca ctttgccgta ccgtttgtcc gatgcggctc cagaacccgg gcacgctccg
     5821 gcgtgcttcg gtcgggattg cggccttcga gtcgcgacgt agcaggtaga taaccggaaa
     5881 gagcacgagc tcgaaaccga gactgacgag aaatagttgc tggtgagtgg tcggttcaaa
     5941 accaaacagg ctgaaccgca aatccagctg ccggatgtag tcgaatatcc agccggcggc
     6001 gatatagccg acgttcatca ccatgtagat cacggagaac gacatcgacc gctgcgctgt
     6061 cgtcgagtag cggcgtgtgg cggcaagcag taccggcgtt ccgagcgcct cgcccaccgc
     6121 gagcggaaga acgccgcatg cgagcgcgag cgacggattc gtcgtcagca ccattacgct
     6181 gcgcgcgacg gtgcagatgg ctacaccgag gaaaaatgtg cggcgcaaac cgatcgcgtc
     6241 tgtgagtgag cccgccagca aggtgaacac ggtcatggcc ggcgcccaaa cccagcccac
     6301 caaagctccc gctgcctggt cgctaaagcc gaggtccttt gacagccaaa ggaccatcgt
     6361 tttgttcgtg atcgaatagg cggtgtaaat gagaaacttg atcaggaacg tgagccacaa
     6421 ctcccgctgc gcgccgcgca gcaccgtgaa cttggcgata aagccgtgaa ctcctgccgg
     6481 cgcgatgggc gtgacaatct cttctgcggg taaggctttg gttgcttcgg ccacgatgtc
     6541 gatcttgcgc tacaacatgt ttgccacaaa ctcccgcgcg tcgaacggcg cgaaattgtc
     6601 gatcttttcg ccggtgccga taaaacgcgt gggcatcccc agctcagctt gaatcgcgac
     6661 tacgattccg ccttcgccgc tgccgtccag ctttgtgacg atcaaaccgc tcaggccgag
     6721 cgcttgatta aattcgcgtg cctgattgag cgcattgccg ccggtcgtcg cgtcgatgac
     6781 gagcagcgtt tcgtgtggcg cgtccgaatc gagtttggcc agagtgcgtt tcaccttttg
     6841 cagttccgcc atgaggttgc ttttcgtgtg caatcgcccg gccgtgtcac acaaaagaaa
     6901 gtcgaccttc tgtttggccg ctttctggta agcatcgtaa caaagcgcag ccggatccgc
     6961 gccatactgg ctgcgcacca tctcgacacc cagccgctcc gcccagatgc caagctgctc
     7021 gatcgctgcg gcgcgaaatg tgtccgccgc ggcgagcaaa actgaatgtc gatgttcgag
     7081 tagaaaatgc gccaacttcg ctgttgacgt ggtcttgccc gttccattga cgcccaggat
     7141 gagcacgacg gcgggccttc ctcttgccgc gcgcagcggc tccgcccgtt tcggcagtac
     7201 cttgagaatc tcttcgcggg cgacttcgat gacattgctg actccgagca gcgaccacgc
     7261 ttcgtgctcc ttggcgcgct cgatgatgcg catcgtcagc ggcacgccga gatccgctcg
     7321 aatgagcgca tgctcgagtt cgtcccaatc gattggcttg tttgcgaatg tctgcgcaag
     7381 cgtttcgaga aacttcattc tgtcatgtcg agcggagtcg agacatctct aattataaac
     7441 agtgagagat tcctcgactt cgctcggaat gacaaagagg aagatcgaca gttactgctg
     7501 cggtggtcct tgcctaacga gcggctcgcg cgccggtcgg tttagctctt tacgaactcg
     7561 gtccagaatt ccgttcacga atttcccgga ttccgcgccg ccgaacgttt tcgccagctc
     7621 gatcgcttcg ttgatcgaga cgaccggcgg aatgtcgtca cgatagagca tttcgtagat
     7681 ggcgagtcgc agcacgttac gatcgaccgt tgaaatgcga ttcaggttgt agttctcggc
     7741 gtatttgccg atgcgctcat cgatctccgg caggtgctcg cccattcctt cgatcagcgg
     7801 ctgcgcaaac tcgcgcacgc tcggcttcac cggacagaag tcccaaaagt cttcgagctt
     7861 ctccgggccg gcgccgtgct gaagatcgcg ccagaaatga tattgaatcg ccgcttcgcg
     7921 cgcgcgtcgc cgtttaccca tgttttttac ggcgcagatt cgagatcgtc cgggcgattt
     7981 ccacggccgc gcctgccgct tccgtcccgc gattgatttt ctcgcccaga caacgctcgc
     8041 gtgcctgcat ttcgttgtca aagctcagca ccgcatttat caccggcaca ccacgatcca
     8101 ccgcgatccg ttgcaaagcg tctgtcaccg accggctcag attctgcgcg tgattcgttt
     8161 cgcccttcaa aatgactcca caggcgatga tcgcgtccgc tttgttccgc gacgccagtt
     8221 ctctcacgac aaccggaatt tcgaacgcac ccggcacccg ctggactgaa atgttcgcgc
     8281 ccggtgcgag ggcccgcagt tcttcggtcg cgtgacagac catcgcatcg acgtaatagg
     8341 aattaaaccg actcgccacg actgcgaact tgtgtggtcg agcactgcgg cgggcgggac
     8401 gcggagcagt tttcgacatg cccagctagc aaccatcgac aatcgctcga gtgcgattgg
     8461 tcgtcacaac gcgtggccca gtttcacttt cttcgtctcg aggtagcgca cgttgtgcgg
     8521 gttcgcttcc gcgcgaatcg gcacctgttc gatcatctca agcccgtagc cttctaggcc
     8581 gaccaccttc ttcgggttat tcgtcaggaa gcggaactgg cgcacgccga gatcaaacag
     8641 gatttgcgcg ccgagaccgt aatcgcgcag atcgctcggg taaccgagct tcgcgttggc
     8701 ttccaccgtg tcgagacctt cctcctgcaa tttgtaggca tggatcttgg cggccagacc
     8761 gatgccgcgg ccttcctgcc gcatgtaaac cagaacaccg ttgccttcct cagagatttg
     8821 gcggagcgcg gcgtgcaact gattaccgca gtcgcagcgc ttcgagccga acacgtcacc
     8881 cgtgagacac tcgctgtgca cccgcaccag cgtcggctta ttcttgtcga tcttaccttt
     8941 gactagcgca agatgatgca tgccgtccaa cttcgaacga tataggtgca actcgaaatc
     9001 gccgtaatcg gtcggcagct ttacgacctg ctcgcgttcg acgagtttct cccgtttccg
     9061 gcgatgcgcg atcaaactct ggatcgtgca aatgcgcagt ccgtgttttt tccggaactg
     9121 catcagctcc gacaatcgcg ccatcgtccc gtcgtcgtgt aaaatttcac aaagcacgcc
     9181 ggccgactgg agtccggcca tttgtgcaag atcgacagct gcttcggtat gaccggcgcg
     9241 gcgcaaaacg ccgccgtctt tcgcgcgcag cgggaagaca tgacccggct gcgccaagtc
     9301 gtctggtgtc gagtgggcgt ttgcgattgc gaggatcgtt gtcgcgcggt cgtaggcgct
     9361 aatgccggtg gtcacgccgc gtgcggcatc caccgaaacc gtgaaatcgg tccgttgcgc
     9421 ctcacgattt tgcgccacca ttcgctgcaa gccgagctgc tcggcgcggt cgttggaaat
     9481 tggcacgcaa atcagtccgc ggccatgcgt cgccatgaag ttgaccgctt cgggggtgac
     9541 cttctccgcc gccatcacca aatcgccctc gttctcgcga tcagcgtcgt cggtcacgat
     9601 gacgatgcgg cctttggcga tatcgctcac cacatcgtca atcgcgtcga actggaaagt
     9661 ctg
//
LOCUS       DASTDG010000153         9777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1175214, whole genome shotgun sequence.
ACCESSION   DASTDG010000153 DASTDG010000000
VERSION     DASTDG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175214"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            852..1328
                     /locus_tag="VFO30_05055"
     CDS             852..1328
                     /locus_tag="VFO30_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418689.1"
                     /translation="MKKTHLLLGITLAWALTSICSAQTTTTSSSTATAGSTAPYTEGP
                     VWTITMVKTKPGMDDDYLKTLAKIYRETNEEAKKQGLIMDYKIMLGDAATPQDFNILL
                     MEEYKNMAALDGLRNKLDPINAKIIGTEDVQRQGAIKRMEIREIMGGKLMREITLK"
     gene            1860..2309
                     /locus_tag="VFO30_05060"
     CDS             1860..2309
                     /locus_tag="VFO30_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9418690.1"
                     /translation="MAQLIIIGYRLSGFSCALTRVPELALTRPIKPPLLTIALLAAIS
                     AVAVQNASSSPAPFYRYTDKALAIYAPPPKYPTDQRGRRPTGRGIVVMEVDEKTGWVT
                     SAKMEKSTGSKLLDDAALEAFKQWRFRPGTVRRVHSPISFEHRAQPQ"
     gene            complement(2652..3080)
                     /locus_tag="VFO30_05065"
     CDS             complement(2652..3080)
                     /locus_tag="VFO30_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9418691.1"
                     /translation="MIAVSRARLSIKPIGAALSLLLCGCASERLAFEGNPMPADQVRT
                     FMQVLTPRREYDTPPQFVHGYAPRYPVQYARTQHWGYALLEFNIEPDGSTSQIRIVAA
                     RALAFGQEAALAVEQWKFTPARKNGQPVGVRVRLPFTFRV"
     gene            complement(3082..4191)
                     /locus_tag="VFO30_05070"
     CDS             complement(3082..4191)
                     /locus_tag="VFO30_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HET9418692.1"
                     /translation="MIKGMPEQWFVRVGEREYGPADLETLREWKYEGRVVATNPVRRT
                     DVDAWTTAADVPGLFDELSSAAAHAQRLTPPPLPQSRTFIQLIAETLRIYGAGLWQFA
                     GLALLIVLPSVCGQLAAAFIHTTSNAKADVASLIAAAFGFCMLVLSMVLWPIYIAAIQ
                     IMTAEFAAGRRLGLFQALNEAVRYWPRVAAGCVFVYGVFFLLIVFGVGIAAVPLVAPN
                     SLLAAVFALGLLVLQVWLFGRFFINVLFWQQYAVLENAGVADALRQSKELARSGRDRP
                     WYRRPMWRGVFIASLWFGFVFIVAILQDWPTIVTYSNQFLTSQDPQLLLQKLTAAQQA
                     RGFDLLRFALGLLQRLLQPLVGIAFVSLYLDSRTE"
     gene            4230..5141
                     /locus_tag="VFO30_05075"
     CDS             4230..5141
                     /locus_tag="VFO30_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9418693.1"
                     /translation="MKLLLIDGHYYVYRSFFAIPNLSNSKGEPTNAIFGFTKTLRLMI
                     KHLRPELGAVFWDEGMPERRVQLQPAYKETRKEMPLPMVPQLEFIQQKLTRLLGFKNV
                     SLPNSEADDLMGCYAIAACKGGIEVVLATNDKDLFQLVGPCVKVYTTAKPDLASPKDA
                     FALLGEGEVTKKWEVRPKLIGDVLALTGDSVDNIPGIGIGRKTAAALIREFGSLENLL
                     IKIDNVKSQRTREKLLAAGDQIQQNRRMVELDCDMELPIPIDELRIQPDYPGLIAALE
                     KCEFKSLLQEVREEAAKTVAAQPELRL"
     gene            5413..6756
                     /locus_tag="VFO30_05080"
     CDS             5413..6756
                     /locus_tag="VFO30_05080"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HET9418694.1"
                     /translation="MEEHDQLLGIRGFVIDAPEFGVLRGRRDGAVIIENGRIADAGDY
                     DDLRRHEWPKPIRWIDHRPAAVFPGLIDCHTHLAQYSAIARGESELLPWLRQHIFPVE
                     REFTGPKARAEAPHFFRELARHGTTTAMVYAAIYEDSCEIGFEAARDSGLRIILGKTM
                     MDLGSYGQLQPKKVVSVSLLESERLCRKWHRAEAGRLEYAFSPRFAVACSEKLMRSAA
                     ELAARFDAYIQTHLAENREELEKVHHLYMSAHDYTHVYEKCGLLTPKTVLGHCIHVNP
                     REIAAIAGAQASVAHCPTSNLFLGSGLMKLDQLLKAGIPIGLGSDVAAGPELNMWRVM
                     RAAIDVQKARSAYELNLRPLRPSEAFYLATTGGARTLGKTNLIGTLDAGKEADLTIID
                     LVSLLPYPSERDTLQHLSVQDVVALCVYRAGPHATLATYVRGKCVFQAPNTMLTA"
     gene            6753..7592
                     /gene="nadC"
                     /locus_tag="VFO30_05085"
     CDS             6753..7592
                     /gene="nadC"
                     /locus_tag="VFO30_05085"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET9418695.1"
                     /translation="MNPDPVDIALQEDVGASDITGDSFVPATALASGRIVARELAIIA
                     GTQTAAEVFRRVDPNTRVDVICPDGATVDAQDVVMQVAGIARALLKAERVALNFLQRL
                     SGVATLTRKFVDAAANKHVKILDTRKTTPGLRVLEKAAVLAGGGANHRFGLFDMVLVK
                     NNHLGAHFGVASFQQALAQFRAKQPDVQIEVEANTLAQVRALLELDGVDVILLDNMKP
                     AQVREAVALGKGKVKFEASGGVTLKNVRQIAATGVDYISVGALTHSAHAIDLALEMTY
                     VGA"
     gene            7579..8217
                     /locus_tag="VFO30_05090"
     CDS             7579..8217
                     /locus_tag="VFO30_05090"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9418696.1"
                     /translation="MSALSVARLRARLGKQNIGREIIVLEETTSTNDAILRRAMPDTP
                     EGLVIFAELQTAGRGQRSNVWESAANKGLWFSILLRPKIDIGESARLSRWAIDAVAAA
                     IQREFGLTATIKPPNDVYVGDKKLAGVLVEMRAQQNTPHLVIAGIGVNVNHSAEDFSD
                     AVRPRAASLSMLLGREVDREMFAVELLRDLDRSYAATFADRGGSSSPSSMVT"
     gene            complement(8269..9366)
                     /locus_tag="VFO30_05095"
     CDS             complement(8269..9366)
                     /locus_tag="VFO30_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET9418697.1"
                     /translation="MVPVEHNDPINAKILAVSEDKIDGFVREPFEEIARRCGVDVDVV
                     MARIAAMLRAGTIRRVRQTLLATNLAEGALVAWKVPAEKIDDAFDWMFQCDPFSGHVV
                     LRSTDTVTAGSDYKLWTTLKVPHGFSLQQHCALLARKVGAEHFRIMPAKGIFTLGVGH
                     VRRKTIPPGSRADQPAKMIPVQIVDLNEREWAVLLALKREFTPAEIRPMPWAARAHEA
                     DVPLDEFYRVAEQLQSRKIIGRFSTFLEHVKPSAAGVRVTRFNALFHWAVPPGREVEA
                     GGEVGRHEILTHCYWREAGPEFRNVNVMAVAHGTDKELLLEHKAAIDRHLEASGILVS
                     YTNVFWGGRSEIKPSEISPRLYGEWFAQQNT"
     gene            complement(9415..>9777)
                     /locus_tag="VFO30_05100"
     CDS             complement(9415..>9777)
                     /locus_tag="VFO30_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET9418698.1"
                     /translation="YTPFALGPLRGHGGFVVLGLIWFAAIFGVILKMTRGTSRHRKLA
                     MSLYLGMGWLIPLTHHSLMLAVPFSALMWLLAGGVAYTAGVLFFINKRLRYSHLIWHL
                     FVLAGTSCHFAAVLSCAS"
BASE COUNT         2163 a         2902 c         2781 g         1931 t
ORIGIN      
        1 tcggtatttc acgtcctcag gtgttagcgc cgggatggac atgacattag ccgtaatcgc
       61 gaagctgtgt ggccgagaaa agagtttgga gatcgcaagt agggcggaat acgagtggca
      121 cgaggatagt tgctgggatc cgtttgccgc tggcgttgag gtgtgattga aaaggctaac
      181 aaggctgcag cgaaccggca ctgagcgttc ccatgtagtt agttcctcga ccttgcggcg
      241 acgtgcgctg tcaggcgcgg tcgctgatct tgtgtctcgt tagacccaca tgcttgcgct
      301 gataaacagg agcctgtcgt tgctggttta tctcggctac atcgctgctg cattcgctcg
      361 aggcggaggt cgtgttgcgt tgacactcgc gattcttctt cttcttcctg tggcttgcat
      421 ttggtttccc cgaccgctgg gcgattacgc cggaacgatc tgcggccagt tcgttacgtc
      481 ttcgacccct gcgtttttag tttgcgcggg cggttggctc gtcctcgtcg gcgtgcctct
      541 gatcgcttac gcattatctg tcgcaccgtg aacaggtcta accaatcgct ggagccaacc
      601 gataatcgag aagaggacga aggatgaatt atgaacgata agctcgccgc agtttacccg
      661 ccggggcggg ccagcggtgg ctcagcttcg tctcgttaga tgagaagcgt catgcggacg
      721 tttccctgtg tcgccagccg tcgctgcgtt ttgcgcttgc gtttgtgcga cgcacccaat
      781 acaaggcacg tgcgtccgag gctctgaggc agccttagtt acgcgaatcg aaaaacaacc
      841 aaagaccata aatgaagaaa acacatctgc tcttaggaat tacgctggcg tgggctttga
      901 ccagcatctg ttcggcacaa acaacgacaa cttcctcctc caccgcgacc gcaggcagca
      961 ctgctcctta taccgaaggg ccagtctgga cgatcacgat ggtaaaaacc aaaccgggga
     1021 tggacgatga ttacctcaag acccttgcca agatatacag ggagaccaat gaggaagcga
     1081 agaagcaagg ccttatcatg gattataaga tcatgctcgg tgatgccgcg actccgcagg
     1141 atttcaacat cctgctcatg gaggagtaca agaatatggc agcacttgac gggttgcgaa
     1201 ataaactcga ccccatcaat gcgaagatca tcgggaccga ggatgtgcag cgccaaggcg
     1261 caatcaagcg gatggagatt cgcgagatca tgggcggcaa gctcatgcgc gaaattactc
     1321 taaagtaagg caaacctaca gatcaagatg gcggctcttg aacaccggag ccgccgtccc
     1381 ttcgacggca gtcgtcagct catctaacca gacgctggca gccaacccgc cacctcgttg
     1441 atcccgaccg attttatgca cgctcttgac tgcaaggtgc tgggtggccg agcttgtgtc
     1501 tcgttagacc tacaagtcgg gcgcataatc aacgagcaag agattgagca cgatgttttg
     1561 agtgctactg atacggtttg gcggaaagtt tgggttagtg tcgcgataac cgttgcgttc
     1621 atcgctacca cgattctgtt agtcagtgtt cctgcgtcac gcttcaccgg ccctgcagtg
     1681 agagagtatg ccgcagggat tgcgcgcgtc attgtcggat tgatcgtgat cgttacaatt
     1741 cggcaacggc attgggcctc taacgcgctc cgtccaccgt cgccacggtc gtgggcgatg
     1801 agaacgcggt cggttcgcga gcttccggcc cgacagtcat tgcagcacgt gctgaggcga
     1861 tggctcagtt aatcatcatt ggttatcggt tatcgggatt cagttgcgct ctcacaagag
     1921 tgcctgaact tgctctcaca agacctatca aacctccact gctcactatt gcacttctgg
     1981 cggcaatttc tgccgtcgcg gtgcagaacg cgtcttcgtc cccagctcca ttttaccggt
     2041 acacagataa agcgcttgcc atttatgcac cgccacccaa atatcctact gatcagcgag
     2101 gaagacgtcc gacaggtcgc ggcattgtcg tcatggaagt cgacgagaag accggctggg
     2161 tcacttcggc gaaaatggag aagagcaccg gcagtaaact tctcgatgat gcggctcttg
     2221 aagcgttcaa gcaatggcga tttcggcctg gcactgttcg gcgcgtccac tcgcccatca
     2281 gttttgagca tcgagcgcag ccgcaatagc tctaaccaac gcctcggcag cgctagtcaa
     2341 gctccgtctc gtcacatggt aactccagca gattatccaa gagtaattac agcattgctt
     2401 ggctacctgg tgtttaccta cgagggctgt cgccaacttt accgcgcgag gcacgtggat
     2461 cgattggagc gagaggggaa gatgtcgccg acttctgcaa cgcgtgttcg caagagaccg
     2521 tggtggggtt ggggctcatc gcagggggca tcgctttcct ttcgccaatt tattcaacgt
     2581 ccgacgccaa agctcgctcc cggcggcgca ttgcattggc tatcccgtat cgcagtgagt
     2641 tgactgcaat tttaaacgcg aaacgtgaac ggaagccgga cacgcactcc taccggctga
     2701 ccgtttttgc gcgccggcgt gaacttccat tgctcgacgg caagagcggc ctcctgccca
     2761 aaagcgagcg ctctcgccgc gacaatccgg atctgactgg ttgagccgtc aggctcgata
     2821 ttgaactcca gcagcgcgta gccccaatgc tgcgtgcgag cgtactgcac tggataacgc
     2881 ggcgcgtagc catgcacgaa ttgcggtggc gtgtcgtatt cgcgccgcgg ggtgagtacc
     2941 tgcataaaag ttctcacctg gtccgcgggc atggggttgc cttcgaaggc aagccgctcg
     3001 cttgcacaac cgcaaagcaa caggctgagc gcggcaccaa ttggtttgat cgacaaccga
     3061 gcgcgcgaca ccgcaatcat gctattcagt tcggctatcc agatagagac taacaaaggc
     3121 gatgccgacg agcggctgga gcaggcgttg caataggcca agcgcgaatc gcagcaggtc
     3181 aaagccgcga gcttgctggg cagcggtgag tttttgcaaa agcagttgtg gatcctggct
     3241 cgtcaaaaat tgattggaat aagtcacaat cgtcggccaa tcctgcagaa tcgcgacgat
     3301 gaagacaaac ccaaaccaaa gcgacgcgat aaagaccccg cgccacatcg gccgacgata
     3361 ccagggccga tcgcgaccgc tgcgcgcgag ttctttgctc tgccgcaacg cgtcggcgac
     3421 gccggcgttt tccagcacgg cgtactgctg ccaaaacagc acattgataa agaaccggcc
     3481 gaacaaccag acctgcagca cgagcaagcc aagcgcgaac actgccgcca gcaacgaatt
     3541 cggcgcaacc agtggtaccg cggcgatacc gacgccaaat acaatgagca aaaagaacac
     3601 gccgtaaacg aacacacagc cggccgcgac gcgtggccag taacgaactg cctcgttcag
     3661 agcctggaac aaaccgagcc ggcgtcccgc agcaaattcc gcggtcataa tttgaatggc
     3721 ggcaatgtag atcggccaca gcaccatgct gagcacgagc atgcaaaagc caaacgccgc
     3781 cgcgattaga ctcgcaacat cggcttttgc atttgatgtc gtgtgaatga atgcggccgc
     3841 cagttgacca cacaccgacg gcaaaacgat cagcagagcg agtccggcga attgccacaa
     3901 gccggcgcca tagatccgca gcgtttctgc gatcagctga atgaacgtgc ggctttgcgg
     3961 caaaggcggc ggcgtcagcc gctgtgcgtg cgcggcagcg ctcgacagct catcaaagag
     4021 gcctggcacg tccgcggctg tcgtccaagc gtcgacatca gtgcgccgca ccggatttgt
     4081 cgcgacgacc cggccttcgt acttccactc gcgcagtgtc tccagatccg ccggcccata
     4141 ctccctttct cctacgcgga cgaaccattg ctccggcatg cccttgatca tcccgcatgc
     4201 aacagccaga atccagcagc cgctgaccga tgaaactgct cctgatcgat ggacattatt
     4261 acgtctaccg gtcgtttttc gcgatcccga acctgtcgaa ttccaaaggc gaaccaacga
     4321 acgcgatttt cgggttcacc aagacgctcc gcttgatgat taagcatctg cggccggaac
     4381 tcggcgcggt attctgggat gaaggcatgc cggagcgccg cgttcagttg cagccagcct
     4441 acaaggaaac gcgcaaagaa atgccgctgc ccatggtgcc ccagctcgaa ttcatccagc
     4501 aaaagctgac tcggctgctc gggttcaaaa acgtgtcgct gcctaattcc gaagctgacg
     4561 acctaatggg ttgctacgca atcgccgcgt gcaagggcgg catcgaagtg gtgctcgcca
     4621 ccaatgacaa ggacctgttc caactggtgg gcccgtgcgt aaaagtctac accaccgcga
     4681 agccggacct cgcctcgcca aaagacgctt tcgcgctcct tggcgaaggc gaagtgacga
     4741 aaaaatggga agtgcggccg aagcttatcg gcgacgtgct tgccctgact ggcgattcag
     4801 tggataacat cccggggatc ggcattggcc ggaaaacagc cgcggccctg atccgtgaat
     4861 ttggcagcct tgagaacctc ctcatcaaga tcgacaatgt gaaaagccag cgcacccgcg
     4921 agaagctcct cgcggcaggc gatcagatcc agcaaaaccg gcggatggtt gagctggatt
     4981 gcgacatgga actgccaatc cccatcgatg agctgcggat tcagccggac tatcccggtc
     5041 tcatcgcggc tctagagaaa tgcgagttta aatcactttt acaggaggtg cgcgaagagg
     5101 cagcgaaaac ggttgcagca cagcctgagc tgcgattgta aacccgaccg cgcagtcgtc
     5161 aggcccaggt gccccgttag agcggcacca gacactcgcc cgaccggcta cgtttttttc
     5221 ggaagaaagt ttcgaacacc gaacgccggc cgctgtccaa gtttacaggt taaggcgatt
     5281 cgggctcctg cacgctagtc ttatctgctg tttctccttg gcgtgcgggg cccgaatctt
     5341 ttttgcccgt cgatgtccgc caactgcgag gttgcccgtc gacgcgccgt gatgttttca
     5401 tagcgcgatt ccatggaaga acatgaccag ctgctcggga tccgcgggtt cgtgatcgac
     5461 gccccggaat tcggggtgct gcgtggccgg cgagacggcg cggtcatcat cgagaatggg
     5521 cgcatcgccg atgccggcga ttacgatgac ctccggcggc acgagtggcc aaagcccatt
     5581 cgctggatcg accatcggcc ggcggccgtc tttccaggac tcatcgattg tcacacgcac
     5641 cttgcgcagt attcggccat tgctcgaggt gagagcgaac tgttgccatg gctgcgccag
     5701 catattttcc cggttgagcg cgagtttacc ggaccaaagg cgcgcgccga agcgcctcac
     5761 tttttccgcg aactggcaag acatggcacg acaacggcga tggtctacgc cgcgatttac
     5821 gaggacagct gcgagatcgg tttcgaagcc gctcgggaca gcggtttacg gataatcctg
     5881 ggcaaaacga tgatggatct cggctcgtac gggcagctcc agccgaaaaa ggtcgtctcg
     5941 gtttcgctcc tggaaagcga gcggctctgc cgcaaatggc atcgcgcgga agccggccgg
     6001 ctcgagtacg ccttcagccc gcgctttgcc gtcgcctgct cggagaaact gatgcgcagc
     6061 gcggcggagc tggcggcgcg gttcgacgcg tatattcaaa cacatcttgc cgagaaccgc
     6121 gaagagctgg aaaaagtgca ccatctttac atgtcggctc acgattacac acacgtctac
     6181 gaaaagtgcg gtctgctcac acctaaaact gtgctcggcc actgcattca tgtgaatcca
     6241 cgcgagatcg cggccatcgc aggcgcgcaa gcgagcgtcg cgcactgtcc aacctcgaac
     6301 cttttcctcg gcagcggcct catgaagctc gaccagttgt taaaagctgg aattcccatc
     6361 gggctcggct ccgatgtcgc cgccggcccg gagctcaaca tgtggcgagt catgcgcgcc
     6421 gcgattgacg tgcaaaaagc gcgcagcgcc tatgagctga atctgcggcc gctgcgaccc
     6481 agtgaggcat tctatctcgc gaccacgggt ggggcacgca ccctcggcaa aacgaatctg
     6541 atcggcacgc tcgatgccgg caaggaggcc gacctaacaa ttattgatct agtcagtctg
     6601 cttccctacc cgagcgagcg cgacactctc caacacctct ccgtgcaaga tgtcgttgcg
     6661 ctttgcgttt atcgcgccgg accgcatgcg acgctcgcca cctacgtgcg gggaaaatgt
     6721 gtctttcagg cgccgaacac aatgctcacg gcatgaaccc agacccagtc gacatcgcgc
     6781 ttcaggagga cgttggtgcc agcgacatta ccggcgactc tttcgttccc gccacagccc
     6841 tcgcctcggg ccgcatcgtc gcgcgagaac tggcgattat cgctggaacg caaaccgccg
     6901 cggaagtttt tcggcgtgtc gatcccaata cacgagtgga tgtcatctgc ccggatggcg
     6961 cgacggtcga tgcacaagat gtggtcatgc aagtggcggg aatcgcccgc gcgctcctaa
     7021 aagcggaacg cgtcgcgtta aattttctgc agcgcctctc cggcgttgcc acattgacgc
     7081 gcaagttcgt tgacgccgcg gcgaacaagc acgtcaaaat cctcgacacg cggaaaacga
     7141 cgccgggctt gcgcgtatta gaaaaggctg cggtgctcgc cggcggcggc gctaatcacc
     7201 gcttcggctt gtttgacatg gtgctcgtga aaaataatca tctcggcgcg catttcggcg
     7261 tcgccagttt ccagcaggca ctcgcgcagt tccgcgccaa acagccggac gtgcagatcg
     7321 aagtcgaagc aaacacgctc gctcaagttc gcgcgttgct cgaacttgat ggtgtcgatg
     7381 tgattttgct cgataacatg aagccggcac aggtccgcga agcggtcgca ctcggaaaag
     7441 gaaaggtaaa attcgaagca agcggtggcg ttacgctaaa gaacgttcgg caaatcgccg
     7501 ccaccggagt cgattacatc agcgtcggcg cgctaacgca ttctgcgcat gcaatcgatc
     7561 tcgcactcga gatgacgtat gtcggcgctt agcgtcgcgc gcctgcgcgc tcgcctgggc
     7621 aaacagaaca tcggccgcga aatcattgtg ctcgaggaaa ccacctcgac caacgatgcg
     7681 atcttgcgcc gcgcgatgcc cgacacgccc gaaggtttgg tgatttttgc cgaactgcag
     7741 accgctggac gcggtcagcg cagcaatgtc tgggaatcgg ccgcgaacaa agggctgtgg
     7801 ttctccattc tcttgcggcc gaagatcgac atcggcgagt cggcgcgcct cagccgttgg
     7861 gcgatcgacg ccgtcgcggc ggcaatccaa cgtgagtttg gtcttaccgc gacgattaag
     7921 ccgccaaacg acgtttatgt tggcgacaaa aagctcgccg gcgtcctcgt cgaaatgcgt
     7981 gcccaacaaa atacgccgca cctcgtcatc gccggcatcg gcgtcaacgt caaccacagc
     8041 gcggaagatt tttctgacgc ggtcagacct cgggccgcat cactgtcgat gctgctcggc
     8101 cgagaagtgg atcgcgaaat gtttgctgtc gaactgttgc gcgacctcga ccgcagctac
     8161 gcggcgacat tcgctgaccg aggtggatcg agctctccga gctcgatggt aacttaaccg
     8221 gcaaacccgc agtttgtgac atcgcccgcg gaagcggccg atccaccctc acgtattttg
     8281 ctgcgcgaac cattcgccat aaaggcgcgg cgaaatctcc gacggcttaa tttcgctgcg
     8341 cccgccccaa aacacgttcg tgtacgaaac gagaatgccg cttgcctcga ggtgccgatc
     8401 gatggcggct ttgtgttcca gcaacagctc tttgtctgtc ccgtgcgcga cggccatgac
     8461 gttgacgttg cggaattccg ggccggcttc gcgccagtaa cagtgtgtca aaatctcatg
     8521 tcgcccaact tcgccgccgg cttcgacttc gcggccaggc ggcacagccc agtgaaacag
     8581 cgcgttgaat cgcgtcacgc gcacaccagc agccgacggt ttcacgtgct ccaggaaagt
     8641 ggagaaccgg ccgatgattt tgcgcgactg caactgctct gcgacgcgat aaaattcgtc
     8701 cagcgggaca tcggcttcat gggcgcgcgc cgcccacggc attggccgga tttccgccgg
     8761 cgtaaattct cgtttcagcg cgagcaacac cgcccattcg cgctcgttaa gatcgacaat
     8821 ttgcaccgga atcatcttcg ccggctgatc cgctctgctg ccgggtggaa tcgttttgcg
     8881 ccggacgtga ccgaccccga gtgtaaagat tcccttcgcc ggcatgatcc ggaaatgttc
     8941 ggcgccgact tttcgcgcga gcaatgcaca atgctgctgc agtgaaaaac catgcggcac
     9001 cttcaacgtc gtccagagtt tgtaatcaga accggcggtg accgtgtcag tcgaccgcag
     9061 gacgacatgg cccgagaacg gatcgcactg aaacatccaa tcaaatgcgt catcgatttt
     9121 ctcagccgga actttccacg cgacgagcgc gccctccgcg agattcgtgg caagtaacgt
     9181 ctgccgcact cgacggatcg tgccggcgcg aagcatcgcc gcgatccgcg ccatcacaac
     9241 gtcgacatct acgccacagc ggcgcgcgat ctcctcgaac ggctcgcgca cgaaaccgtc
     9301 gattttgtct tccgagactg cgagaatttt cgcgttgatc ggatcgttat gctcgaccgg
     9361 gaccatcaag gtaattagcg cctcgagcgc ttttcttcaa ttgatgggcg actgttaact
     9421 cgcgcacgac aacaccgcgg caaaatgaca gctcgttcca gcaagcacaa acaagtgcca
     9481 aataagatga gaatagcgga gccgcttatt aatgaagaac agaacgcccg cggtgtaagc
     9541 gacgccgcct gcgagcagcc acattagcgc agagaatgga acggcgagca tgagcgaatg
     9601 atgagtaagc ggaatcagcc agcccatacc cagataaaga gacatcgcca attttcggtg
     9661 ccgcgacgtt ccgcgcgtca tcttcagaat cacgccaaat atcgcggcaa accagatcaa
     9721 gccaagaacc acgaacccgc cgtggccgcg cagtggcccc agcgcaaacg gcgtgta
//
LOCUS       DASTDG010000154         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_336517, whole genome shotgun sequence.
ACCESSION   DASTDG010000154 DASTDG010000000
VERSION     DASTDG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336517"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFO30_05105"
     CDS             <1..185
                     /locus_tag="VFO30_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418699.1"
                     /translation="EHSCAVTFVASGAYEHSAPPPVVPAPAAITFMRVADLNPVRVRS
                     LFLLASIFEHAPPVLA"
     gene            353..2479
                     /locus_tag="VFO30_05110"
     CDS             353..2479
                     /locus_tag="VFO30_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9418700.1"
                     /translation="MKKFLIASVAMLCGASMATAQDAVTASASPAPAATPGTSTAENV
                     VITSQELDISREAIVPNLGATRYTVGPDRLDSQAQGEDAPFNQTILRFPGVAQDSFGQ
                     LHIRGEHANLQYRIDDVLIPESIPGFGQELETRFADNISLITGALPAQFGFRNTGVID
                     IHTKNGPVFTGDEASVRVGSFDTIMESFERGGVIGKLSYYVTESYLHDGIGVENPTRS
                     SSPIHDDTDQYKFFGYFSDIIDNTSRVTLLVSGNHSDFEIPNNPGQPPAFNDMGRSTF
                     DSAKLDENQSEDSAYEILTYQKKAGDLDFQASIFNRYSSILFQPDNVGDLIFNGVASR
                     VDRAILSNGVEFDSSYKLNDQHTIRAGLIFTEQHATIDTATLVFPVDANGNQTSTTPL
                     RIVDNHDKYGYFYGFYLQDEWKPFEQLTINFGGRLDFVNAFADENQLSPRVNIVYKPL
                     DGTTLHAGYARYFTPPPLEAVPQSTIAKFAGTTNESAVTADSAVTSERAHYFDAGVTQ
                     KIMEGFNVGLDGFYKSSHSVLDEGQFGQALILSSFNYKRGEIYGGEFTSSYDHGPFSA
                     YGNLAYEWARGTQVSSAQFLFDPDELAYINKHWVFLDHDQRFTGSAGISWTWNDWKVS
                     SDFLYGNGLRRGFANTQKGHPYETINASLQRAFTIPKVGTLKVRFDVVNLFDKIYELR
                     DGSGIGVGAPQFGQRRGFYGTLAYDF"
     gene            2551..>2791
                     /locus_tag="VFO30_05115"
     CDS             2551..>2791
                     /locus_tag="VFO30_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418701.1"
                     /translation="MRNFVLATVIVFSLPIASAQQLTPNPSSTPAASSDVESLRQQVQ
                     ALTETVKTLQQQVQDQQARIDRMNEENAPEQPEPSP"
BASE COUNT          625 a          835 c          729 g          602 t
ORIGIN      
        1 tcgaacacag ctgcgcggtg acgttcgttg cttcgggcgc ctatgagcat tccgctccgc
       61 cacccgttgt tccggcaccg gcggcaatca catttatgcg ggttgctgat ctgaacccag
      121 ttcgggttcg atcactcttt ctgctcgcca gcattttcga gcacgcgccg cccgtgcttg
      181 cttagcacga aagccgaacc gcgtcttcgc gcggagcgtt aaacttccat tcacttcgcc
      241 tgagtcgcga cgcgcctttt gtgcagaggc gccgcttttc actggcttcg cgcgaatcgc
      301 gaatgccgca aacaatcacg actttgggaa atccatttca aaaacacagt aaatgaaaaa
      361 atttctgatc gcatcagtcg caatgctctg tggggcgagc atggccactg cgcaagacgc
      421 tgtcacggcg tctgccagcc ccgcaccagc tgccacgccg ggtacttcca cggccgagaa
      481 cgtcgtgatc acgagccagg agctcgacat ttcgcgggag gcgatcgtgc cgaatctcgg
      541 cgcgacgcgt tacaccgtcg gcccagaccg gctcgactcg caggcgcaag gcgaggacgc
      601 gccgttcaat caaaccattc tccgatttcc cggtgtcgcg caggattcat tcggtcaact
      661 gcacatccgc ggcgagcacg caaatttgca atatcgcatc gacgacgtgt tgataccgga
      721 aagtattccc ggttttgggc aggaactcga gacgcgtttt gccgataaca tttcgctcat
      781 caccggtgcg ctgccggccc aattcgggtt tcgcaacacc ggcgtgatcg acattcacac
      841 gaaaaacggg ccggtcttca ccggcgacga agcgtccgtc cgcgttggga gtttcgacac
      901 catcatggag agcttcgagc gcggcggcgt gatcgggaag ctcagctatt acgtcacgga
      961 aagttatctg catgacggca tcggcgttga aaatcccacg cgcagcagca gcccgattca
     1021 tgatgacacc gatcagtaca aatttttcgg ttatttttcc gacattatcg acaacaccag
     1081 cagggttacg ctgctcgtaa gcggcaatca cagcgatttc gaaatcccga acaatccggg
     1141 tcagccgccg gcatttaacg acatgggaag atcgacattc gattcggcca agctggacga
     1201 gaaccaatcg gaggattctg cctacgaaat tttgacttac caaaagaagg cgggcgacct
     1261 cgatttccag gcatcgatct ttaatcgcta cagctcaatt ctttttcagc ccgataacgt
     1321 tggcgatctc atcttcaacg gggtcgcctc gcgagttgac cgcgccattc tttccaacgg
     1381 tgtcgaattc gattcgagtt acaaactcaa cgaccagcat acgattcgcg ccggtcttat
     1441 cttcaccgag cagcacgcga ccatcgatac ggctacgctc gtgtttccgg tcgatgccaa
     1501 cggcaatcaa accagcacga cgccgctacg catcgtcgat aatcacgaca agtacggcta
     1561 cttctacggc ttctatttgc aggatgaatg gaagccgttc gagcagctca ctattaactt
     1621 cggcggccgg ctcgacttcg tgaatgcgtt tgcggacgaa aaccaactca gtccgcgcgt
     1681 caacattgtt tacaagccat tggacgggac cacgcttcac gccggctacg cgcgttattt
     1741 cacgccgccg ccgctggaag ccgttccaca aagcaccatc gccaagttcg ccggcacaac
     1801 gaacgagtct gccgtcacgg cggattctgc ggtcacttcg gagcgcgcac actatttcga
     1861 cgcgggcgtc acgcaaaaga tcatggaagg tttcaatgtc gggctcgatg gcttctacaa
     1921 gagctcacat tcggttctcg acgaaggcca gttcggccag gcgctcatcc tttcctcgtt
     1981 caattacaag cgcggcgaaa tctacggtgg cgaatttacc tcgagctacg accatggccc
     2041 attctctgcc tacggaaatc tggcctacga gtgggcgcgt ggcacccaag tcagttccgc
     2101 gcaattcctg ttcgaccccg atgagctggc ctacattaac aagcactggg tttttctcga
     2161 tcatgaccag cgcttcaccg gctcggccgg aatctcctgg acttggaacg actggaaggt
     2221 ctccagtgat tttctttacg gcaatggtct gcgcagaggt ttcgccaaca cgcaaaaagg
     2281 tcatccatac gagaccatca atgcctcgct tcagcgcgcc ttcaccattc caaaagtcgg
     2341 gacactcaaa gttcgtttcg atgttgttaa cctgttcgac aaaatttacg aactgcgcga
     2401 cggcagcggc atcggagtgg gcgcaccaca attcggccag cgccgcggct tttacgggac
     2461 gttggcctat gacttctaga ggctaaacaa agcgcatcac gctcgcgtgc gtgtcggcgc
     2521 ggtgttatcg gtgtccctca agaggtgaaa atgaggaact tcgttctcgc aaccgtcatt
     2581 gttttttctt taccgatcgc ttcggcacaa cagctgacgc cgaaccccag ttcgacgcct
     2641 gctgcttcat ccgacgtcga gagtttgcgc caacaagtgc aggcgctcac cgagacggtg
     2701 aagaccttgc aacaacaggt gcaggatcaa caggcaagga tcgatcgtat gaacgaggag
     2761 aatgcgccgg agcaaccgga accatcgccg a
//
LOCUS       DASTDG010000155         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_858386, whole genome shotgun sequence.
ACCESSION   DASTDG010000155 DASTDG010000000
VERSION     DASTDG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858386"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFO30_05120"
     CDS             <1..595
                     /locus_tag="VFO30_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET9418702.1"
                     /translation="DADSINAVQVAQILKNCGIAALTVHGRTRAQGYSGSADWDVIGE
                     VAAAVPIPVIGNGDLLSPADVARRRRETAIAGAMIGRAAMSAPWVFREIKSYLAIGDI
                     VGPPSFAERWDVVLRHCELAVREWGAEEPAIRSMRARLMAYSRSMPEAKRLREKFSHV
                     SSLAEVRAIAEKGRRAAESPMPGPSEIENQISVSLIS"
     gene            605..976
                     /locus_tag="VFO30_05125"
     CDS             605..976
                     /locus_tag="VFO30_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9418703.1"
                     /translation="MNAESPPLNYKIGNERLIRVTEKAAQQLASLLAQKGTPNGALRL
                     AVVGGGCSGLQYVMDLVEGPKERDILVPTSNVRLVVDPKSALFVSGSVLDYSEDLQKG
                     GFKVTNPNAVAHCSCGESFAV"
     gene            complement(1046..1312)
                     /locus_tag="VFO30_05130"
     CDS             complement(1046..1312)
                     /locus_tag="VFO30_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418704.1"
                     /translation="MRAEAGNSSDHFDRHFHRSDDQKIDDEEEGRHCERGRVRFAERI
                     AEEEEGGEEGSRNLSDRIAYGDRSDRIACRDAVKARLRRAENCI"
     gene            1545..1808
                     /locus_tag="VFO30_05135"
     CDS             1545..1808
                     /locus_tag="VFO30_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418705.1"
                     /translation="MKKILVVFSTVLALSILAITAQENAGGKKTETAGATKAGATSEH
                     KILSPDQVQRGETPPGLPAGAQMAVLDGNPAQKGSFTVRLKMP"
     gene            1818..2069
                     /locus_tag="VFO30_05140"
     CDS             1818..2069
                     /locus_tag="VFO30_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9418706.1"
                     /translation="MAPHTHPTTERVTVISGTGHLGTGDKFDETAGHDMAPGSFAVLP
                     AGMKHFAWSTGETVLQIQSEGPFKIKYVNPADDPRNAKQ"
     gene            2284..2508
                     /locus_tag="VFO30_05145"
     CDS             2284..2508
                     /locus_tag="VFO30_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418707.1"
                     /translation="MTKTDLIVWGISAIMAIGVGWSRYVKHKTRERLVTELAAMEREK
                     REKILSRLNPKEAIELRERLMRRFPAQSDV"
     gene            2611..>2812
                     /locus_tag="VFO30_05150"
     CDS             2611..>2812
                     /locus_tag="VFO30_05150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9418708.1"
                     /translation="MTVPVLPADIDEQKHVNNTVYLRWAQDVATAHWKTLASAEAQAA
                     IRWVVLRHEIDYKAPACLGDEVL"
BASE COUNT          591 a          843 c          827 g          551 t
ORIGIN      
        1 ggacgccgac tcaatcaatg cggtacaggt cgcgcaaatt ctgaagaact gcggtatcgc
       61 ggcgctcact gtgcacgggc gcacccgcgc gcaaggttac tccggcagcg ccgattggga
      121 tgtgatcggc gaagtcgccg cagcggtccc aattccggtg attggcaacg gcgatctctt
      181 aagtcccgcc gacgtcgctc ggcggcggcg tgaaacggcg attgccggcg cgatgatcgg
      241 gcgcgcggca atgagtgcgc cgtgggtttt ccgggagatc aagtcttatc tcgcaatcgg
      301 cgatattgtc ggtccaccga gttttgctga acgatgggac gtcgtcctgc gccattgtga
      361 gctcgcggta cgcgagtggg gcgcagagga accggcgatc cgttccatgc gcgctcggct
      421 catggcctat tcgagatcca tgcccgaagc gaaacggctg cgcgaaaaat tttcccacgt
      481 gtcgagcctg gccgaggttc gagccattgc agagaaaggt agacgcgctg ccgaatcgcc
      541 catgcccgga ccgtcagaaa tcgagaacca aatttctgtt tccctgattt cctgatttag
      601 cccgatgaac gccgaatcac ctcctctcaa ttacaagatc ggcaacgagc gcctgatccg
      661 cgtcaccgaa aaagcggcgc aacaactcgc ttcactgctg gcgcaaaagg gcacgccgaa
      721 cggtgcgtta cgcctggcgg tggtcggcgg cggctgctcg ggtttgcagt acgtgatgga
      781 tttggtcgag gggccaaaag agcgcgatat cctcgtaccg acatcgaatg tgcgattggt
      841 tgtcgatcca aaaagcgcgc ttttcgtcag cggctcggtg ctcgactaca gcgaagacct
      901 gcaaaaaggc ggattcaaag taaccaatcc gaacgccgtc gcgcattgct catgcggcga
      961 aagcttcgca gtctagaacc ccgcccgaaa aaagcaatgc ggcggctcag ctggctggcc
     1021 gccgccagct gagcgccgca cgcaatcaaa tacagttctc cgctcggcgc agcctcgcct
     1081 ttacggcgtc gcggcaggcg attctgtcgc ttctgtcgcc gtaggcgatg cggtcggaga
     1141 ggtttcggct tccttcttcg ccgccttctt cttcttcggc gattcgctcg gcgaagcgga
     1201 ctcttccgcg ctcgcagtgg cgcccttctt cttcatcgtc gattttttgg tcgtcgctgc
     1261 ggtggaagtg ccggtcgaag tggtcgctgc tgtttcctgc ttctgcgcgc aagaaacaac
     1321 aaacgccgtc gcgatcacgg ccacaatcaa ggatagcaat gatttcttca taggtgtgtg
     1381 gtcaagattt gttccagcgc cagaattgta agttgccgca gacagtgcaa gcacattctt
     1441 tcgccttaac aacgcgcaca acgcattgga gcgcggccgc tgcgcagaac aaccgcgcag
     1501 cacggccaga ttcaaagtcg acttcgaatc aacggtgcga acatatgaaa aaaattctag
     1561 tcgtattcag cacggttctg gcgctgtcga tcttagccat cacagcgcag gaaaacgccg
     1621 gtggcaagaa gaccgagacg gccggcgcga cgaaagccgg cgcaacctcc gagcacaaga
     1681 tcctcagtcc cgatcaggtg cagcggggcg agacgccccc aggtttacca gcaggcgcgc
     1741 aaatggcggt gcttgatggt aatccggcgc aaaaaggttc attcactgtg cggttgaaga
     1801 tgccctgagg ctacaagatc gcaccgcata ctcatccgac caccgaacgt gtcacagtga
     1861 tttccgggac ggggcacctg ggcacgggtg acaaattcga cgagaccgcc ggtcacgata
     1921 tggcgccggg cagtttcgct gttttaccag caggtatgaa acatttcgcc tggtcgaccg
     1981 gcgaaaccgt gcttcagatc cagagcgaag gtcctttcaa aatcaagtac gtgaatcccg
     2041 ccgacgatcc gcgaaacgca aagcaatagg ctgcttgaag tcatagccac gccgctgcct
     2101 gccacgccgg agcagcgcga aggcgggtgc gtcgtgcgct tctccggcag cggcgagact
     2161 gcgtaatctc gcgcgcgcta cacgacgggc cacagacccg tggcggcagg ttctgctctg
     2221 cgcttgccct ggtcactccg tttttcactt gccactcggc acgcggcact cgtcacttag
     2281 gtcgtgacca agaccgatct tatcgtctgg ggcatctcgg ccatcatggc gatcggcgtc
     2341 ggctggagcc gttacgtaaa acacaaaacg cgcgaaaggt tggtcaccga gctcgcggcg
     2401 atggaacggg agaagcgcga aaaaatccta agccgcctca atcccaaaga agcgatcgag
     2461 cttcgcgagc gcctcatgcg gcgttttccg gcgcaatcgg acgtgtgacc tccgcggtca
     2521 gacacggccg ccgctgacct cttcctccgc tctccgctct ttgctgataa cagtgctcgc
     2581 ataaattccc gccgcatgca gacgttcgaa atgacagttc cggttctgcc tgcggacatc
     2641 gacgagcaaa agcacgtcaa caacactgtc tatttacgct gggcccagga cgtcgctacc
     2701 gcccattgga aaacgctcgc cagtgcggaa gcgcaagcgg cgatccgctg ggttgtgtta
     2761 cggcacgaga ttgactacaa ggcgccggct tgtctcggcg atgaggtcct ct
//
LOCUS       DASTDG010000156         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1100787, whole genome shotgun sequence.
ACCESSION   DASTDG010000156 DASTDG010000000
VERSION     DASTDG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100787"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..>2423)
                     /locus_tag="VFO30_05155"
     CDS             complement(77..>2423)
                     /locus_tag="VFO30_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418709.1"
                     /translation="KAELISFPEAMSRAKNAGIQIAEVKAPAYSPYFYFEDSDKEHAV
                     WFLDVVTFLNELRELRNERAGGFALYRLGSEDPAIWDALTIPPDFKIDNTTRESLELL
                     KGTDTITDVGEGEIVTVDESRSDGRRTLAVDADGYLTAKYIRFPEFPTLYHQGAGGEH
                     QVAITFDDGPDPHWTPKILDILKAANLKAAFFLTGVNAERYPALVRRIVNEGHEIGNH
                     TYYHPNLALCWPEHVRLELNATQLLLETITGRATTLFRPPYAADTSPSQLSELAPLQI
                     AQDLNYLVVLENIDPQDWAKPGADIIVQRIKQQRRDGSIILLHDGGGDRSQTVEALPR
                     VLSWLHTRGDTVVPLSQLLGLSRDAVMPPLADGARSLTRLVSSTGFRLYHTVEEFLWA
                     FMIVTTALVVIRTLVVVWLAYRFHPAPKLNIAEPVSVLMAAYNEEKVIGDTLRALLET
                     DYRGDLEVIVVDDGSGDGTAAKVEQIAHADARVRLFQQENRGKARALQRAIAAARHGI
                     IVFVDADTRFQRDTLRLLLAPFADQQIGAVSGHAKVGNLRKFIARCQSLEYTCGFNLD
                     RRAYNRWNCITVVPGAVSAIRKRAIMGAGGLSLQTLAEDTDLTLSLHKNRQRIVYVPN
                     AIAWTEAPETVRTLARQRFRWAYGTLQCLWKHREMVFNRNYGALGWLSLPSIWFFQIM
                     LVAVTPIVDLLLLGSLPFGAWRAVMPFVVTFLAMDLVLATLACVLEREPVLHAWRILP
                     MRLIYRPMLSYCIWKAILRALKGVWVSWGKLERTASVPARA"
BASE COUNT          521 a          737 c          684 g          481 t
ORIGIN      
        1 aacaacgaat tcgcgttcaa ctcatcaaca gcgcggccaa gcaaatccca actccatcgc
       61 cgaacatcaa atttgattac gcgcgcgccg gcacgctcgc ggtacgctcg agtttacccc
      121 agctcaccca tacccctttg agcgcgcgca agattgcttt ccagatgcag taactgagca
      181 tcggccggta aatcaaacgc attggcaaga tacgccaggc gtgcagaact ggctcgcgct
      241 cgagaacgca cgccagcgtc gccagcacga ggtccatggc gagaaaagtg accacaaacg
      301 gcatcaccgc gcgccatgca ccgaacggca gcgacccaag caaaagcaaa tcgacgatgg
      361 gtgtgaccgc cacgagcatg atctggaaaa accagatgct cggcagactc aaccagccga
      421 gcgcgccata gttccggttg aacaccatct cgcgatgttt ccacaggcac tgtagcgtgc
      481 catatgccca tcgaaaccgc tgacgcgcga gcgtgcgcac ggtttccggc gcttcggtcc
      541 acgcaattgc gttcggaaca taaacgatcc gctgccggtt tttgtgcaat gaaagggtca
      601 ggtctgtatc ttcggctaag gtctggagac taagcccgcc agctcccatg atcgctcgtt
      661 tgcgaatggc gctcaccgcc ccgggcacaa ccgtgatgca gttccagcga ttgtaagcgc
      721 ggcggtcgag attgaatcca caggtgtact caagcgactg acatcgcgcg atgaacttgc
      781 gcagattgcc gaccttggca tgacctgaaa cagcaccgat ctgctgatca gcgaacggcg
      841 cgagcaacaa ccggagcgtg tcgcgctgga aacgcgtgtc ggcatcgaca aagacaatga
      901 tgccgtgccg ggcggctgcg attgcccgtt gcaacgcgcg cgctttcccg cggttttcct
      961 gttggaacaa gcgcacgcgt gcatcggcat gggcgatttg ctccactttc gcggcggttc
     1021 cgtcgccgga accgtcatcc accacgatca cttccagatc gccccgataa tctgtttcga
     1081 gtaaagcgcg cagtgtgtcc ccgatcacct tttcctcgtt gtaagccgcc atcaggacgc
     1141 tgaccggctc ggcaatgttg agcttcggtg ccggatggaa gcgataggca agccaaacga
     1201 caacgagcgt gcgaatcacc acgagcgccg tcgtcacgat catgaacgcc caaaggaatt
     1261 cctcgacggt atgatagagg cgaaatccgg tgctactgac gagacgggtg agcgatcgtg
     1321 cgccgtctgc gagcggcggc atgacggcat cacgcgaaag ccctagcagc tgactcagcg
     1381 gcacgactgt gtcgccgcgc gtgtgcagcc aactgaggac gcgcggcaat gcctcgaccg
     1441 tttgcgaccg atcgccgcca ccatcgtgca acaaaatgat gctgccatca cggcgctgtt
     1501 gcttgatgcg ctgcacaatg atgtccgccc cgggtttcgc ccagtcctgc gggtcgatgt
     1561 tctccaggac aacgaggtaa ttgaggtcct gcgcaatctg cagcggcgcc agttcgctga
     1621 gctgcgacgg actcgtgtcg gccgcgtagg gcgggcgaaa caacgttgtc gcgcggcccg
     1681 taatcgtttc cagcaaaagc tgtgtggcgt tgagttccag ccggacgtgc tccggccagc
     1741 acaacgccag gttcggatgg taataggtgt gattaccgat ctcgtgtcct tcattgacga
     1801 tccggcgcac gagcgccggg tagcgctccg cgttcacacc ggtcaagaaa aacgcggcct
     1861 ttagattggc cgctttcaga atgtccaaaa ttttcggtgt ccaatgcgga tccgggccat
     1921 catcgaaagt gattgcgacc tggtgctcgc ccccggcgcc ttgatgatag agcgtgggga
     1981 attccgggaa gcgaatgtat ttcgccgtca ggtaaccgtc cgcatcgact gccaacgttc
     2041 gccggccatc ggaccgactt tcgtccactg taacgatctc accttcgcca acgtcggtga
     2101 ttgtgtctgt gcccttcaac aactccagtg attcgcgcgt tgtattgtcg atcttaaaat
     2161 caggtggaat ggtcagcgcg tcccagatcg ccgggtcttc gctgccgagt cggtagagcg
     2221 cgaatccgcc agccctctcg ttgcgcagct cgcgcaactc gttgagaaac gttacaacat
     2281 ccagaaacca gacggcgtgt tccttatcgc tatcttcgaa atagaaatac gggctgtaag
     2341 ccggcgcctt cacttcggcg atttggatac ccgcgttttt cgcgcgactc atcgcttcgg
     2401 gaaaactgat cagctcagct ttt
//
LOCUS       DASTDG010000157         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1501108, whole genome shotgun sequence.
ACCESSION   DASTDG010000157 DASTDG010000000
VERSION     DASTDG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501108"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VFO30_05160"
     CDS             <1..1458
                     /locus_tag="VFO30_05160"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET9418710.1"
                     /translation="PELSFAVRYLNASAGAVITASHNPPHDNGYKVYFADGGQVVEPH
                     ASAIIAKVNAVPRETYEPLPQDRQGKVMTLGADIDEAYMERLQSLIVDRSVIDDAESL
                     RIIFTPLHGTGSVTIKPLLERLDFTFQVVKEQEQFDPRFSTVKSPNPENAEALTMGIK
                     LAEQTNADIVIATDPDCDRLGVAVRSASGEMSLITGNQIGSLLAYYRIKKFFDLGILN
                     EENASRAVIMKTFVTTDLQKVIAEYYGVRCVETLTGFKYFAAKLEKYERALPKEIRKG
                     YRQLSEQESRAVRLKHSSFYVFGSEESYGYSGADFVRDKDANAGAIMFCEMAAYAKSR
                     GQTVDQLLDEIFATFGYFAEKNGSLYFEGAEGAKKIERLLESYALAPPREMLGSNVTS
                     IRNFETDTIRDAEGDEIPKQKMLIFELANGTRIAVRGSGTEPKIKYYMFAQRRPGERR
                     FTAAELEQIKTETDEQLEAIWNWLQTDAQTRLEKK"
     gene            1501..1812
                     /locus_tag="VFO30_05165"
     CDS             1501..1812
                     /locus_tag="VFO30_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418711.1"
                     /translation="MRRDINLSGGEISILKTLGLSGAPVNGKLIIERIGEMEPAEFVD
                     ELNGLITLGYVLADKVNIRTMQDVEKSVFRVNASYAHDLRDAIRPGRRREQERRRRRR
                     G"
     gene            1837..3495
                     /gene="lnt"
                     /locus_tag="VFO30_05170"
     CDS             1837..3495
                     /gene="lnt"
                     /locus_tag="VFO30_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET9418712.1"
                     /translation="MREFATKATRVWPWLAATATGLLCTACFPPFNIGWICWIALTPL
                     ISAIWFSRGDAKRRWLRNLLLGYVGGLVFFTAAFSWLGSLGVLFESPPLRGLSFLLAI
                     YLGMHFAFWAWFVGLITPREFLSSGKNLLVALVAASAWGAHEWVRGWLFSGFGWNGFG
                     VALYANWPMIQIAEFTGVAGLSFVIAFANIIAVTTALRLFHETRTRRMQPHFDLTLTM
                     LALVGLFAFGLHRIQFPARATPLRVAAVQPNVPQLQKFDPQFARTIFERFANYSAIAL
                     QANPPVDLIVWPESSMPDLVRDQTTLSYRFVMDFASSSRADLLLGALDSEDRHDYNAA
                     LLVAAGGHAMQIYRKLHLVPFGEYVPLRHSFPLFAAIASRWVPADFEVGREFTVFRLT
                     NANVSVAPLICFEDTIGELTRRFVLRGANLLVNMTNDAWYLQSAGSQQHLANAVFRCV
                     ETRRPMLRAANTGVTCFINEFGRITQVLRDENGGTFTEGVLTGEVNVPQEPALSDSRT
                     GRQLTFYTRHGELFAKICAGVTAIAVVSAVLRRRWIGRSAGDVK"
     gene            3576..>4188
                     /locus_tag="VFO30_05175"
     CDS             3576..>4188
                     /locus_tag="VFO30_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET9418713.1"
                     /translation="MAVQLPLDHLPELWPKQFRGAKIGALLHPASVSSNLEHASRILE
                     RLNGDLFRLVAFFGPQHGYLGQTQDNMVEWKSYEHPRLRIPVHSLYGEHREPTPEILR
                     GIDVLLVDLQDVGARYYTFIWTLYLCMRVCEAHGVAVVVLDRPNPIKGLTTEGPVLDP
                     AYQSFVGMHPIPVRHGRTIGELAQQFRDEAFPKCRLSIFRMSAR"
BASE COUNT          876 a         1198 c         1216 g          898 t
ORIGIN      
        1 ccggaactct ccttcgcagt tcgctactta aacgcgagcg ccggcgctgt catcaccgca
       61 agccacaacc cgccgcacga caacggctac aaggtttact tcgccgatgg cggacaagtc
      121 gtggaaccgc acgccagcgc aatcatcgcc aaggtcaacg cagtgccgcg cgagacttac
      181 gagccgctgc cgcaagatcg acaaggcaag gtaatgacgc tcggcgccga cattgacgaa
      241 gcctacatgg agcggctcca atctctgatt gtcgatcgaa gcgtgatcga cgacgcggaa
      301 tcgctgcgga ttatcttcac gccgctgcat ggcaccggca gcgttaccat caaaccgctg
      361 ctcgagcggc ttgatttcac ctttcaagtg gtgaaggagc aggagcaatt cgatccgcga
      421 ttttcgaccg tgaaatcgcc caacccggaa aacgcggagg cgctcaccat gggcattaag
      481 ctcgcagaac aaacgaacgc cgacatcgtg atcgcgaccg atccggactg tgatcggctg
      541 ggcgtggcgg ttcgcagcgc gagcggtgag atgagtttga tcacgggcaa ccagatcggt
      601 tcgctcctgg cgtattaccg aatcaaaaaa ttcttcgacc tcggcattct gaacgaggag
      661 aacgcctctc gcgcggtgat catgaagacg ttcgtcacga cagatctcca gaaagtgatc
      721 gccgaatact acggcgtgcg ctgcgtcgag acgcttaccg gattcaaata tttcgcagcg
      781 aagctggaga agtatgagcg tgcgttgccg aaagagatcc ggaaaggtta tcggcagctg
      841 tcagaacagg aatcgcgcgc ggtccggctc aagcactcat cgttttacgt ttttggcagt
      901 gaagagagct acggctacag cggggcagat tttgtgcgcg acaaggatgc gaacgccggc
      961 gcaatcatgt tctgtgagat ggccgcgtat gcgaaatcgc gcgggcaaac ggtcgaccag
     1021 ttactcgatg aaatcttcgc tacctttggc tatttcgccg agaaaaacgg atctctctat
     1081 tttgaaggtg cggaaggcgc gaaaaagatc gagcggttgc tcgaatcata cgcgttggcg
     1141 ccaccgcgcg aaatgctcgg ctcgaacgtg acaagcatcc gcaatttcga gactgatacc
     1201 atccgcgacg cggaaggcga cgagattccg aaacagaaga tgttgatctt tgagttggcg
     1261 aacgggacgc ggatcgcggt gcgcggttct gggactgagc cgaagatcaa atattatatg
     1321 ttcgcgcagc gtcgaccggg cgaacgcaga ttcactgccg ccgaactcga acagatcaaa
     1381 accgaaacgg acgagcagct tgaggcgatc tggaactggt tgcaaacgga tgcgcagacg
     1441 cgtcttgaaa aaaaataacc tgctcgacag cgtcggtttc gttgcgaaac ttcacttcgt
     1501 atgcgtcgcg acatcaatct gagcggaggc gagatctcga ttctgaaaac gctcggcctt
     1561 tccggtgcgc ctgttaacgg caaactcatc atcgaacgca ttggtgaaat ggaacccgcc
     1621 gagtttgtgg atgaattgaa cggactgatt acgctcggct acgtgctcgc cgacaaagtg
     1681 aacatccgca caatgcaaga cgtggaaaaa agtgtctttc gcgtcaacgc ctcctacgca
     1741 cacgacctgc gtgacgccat tcgcccaggc cgtcgccgcg aacaggaaag acgacgccga
     1801 cgacgcggct gagtcgcagg cattcggcga atcaacgtgc gcgaattcgc gaccaaggcg
     1861 acgcgcgttt ggccgtggct ggctgcaacc gcgaccggcc tgctctgcac agcctgtttc
     1921 ccgccattca atatcggctg gatttgctgg atcgcgctga ctccgctgat cagcgcgatc
     1981 tggttttccc gaggagacgc aaagcgccgt tggctgcgga atctcttgct cggctacgtt
     2041 ggcggactcg ttttctttac ggccgcattt agctggctcg gttcactcgg cgttctgttc
     2101 gaaagtcctc ctcttcgcgg actctccttt ctgttggcga tctatctcgg aatgcatttc
     2161 gcattctggg cgtggttcgt cggactgatc acgccgagag aatttcttag ctcggggaaa
     2221 aatctgctcg tggccttggt tgcggcgagc gcatggggcg cgcatgaatg ggtgcgcggc
     2281 tggcttttca gtgggttcgg ctggaacggg ttcggcgtcg cgctctacgc gaactggccc
     2341 atgattcaga tcgcggaatt taccggcgtc gctggtttat ccttcgtgat tgcgttcgcg
     2401 aacatcatcg cggtcacgac agcgctccgg ctcttccatg aaacccgcac gcgccgaatg
     2461 cagccgcatt tcgacctgac gctgacgatg ctcgcactcg tgggactttt cgccttcggt
     2521 ttgcacagga tccaatttcc agctcgcgcc acgccgctgc gggttgcggc tgtgcagccc
     2581 aatgttccgc aattgcaaaa gttcgacccc caatttgccc ggacgatttt cgagcggttc
     2641 gccaactata gcgcgattgc gctgcaggcg aacccgccag tcgatctgat tgtttggccg
     2701 gagagttcca tgccggacct ggtgcgcgat cagacaactc tgagctatcg attcgtcatg
     2761 gattttgcca gttcgagtcg cgccgacttg ttgctcggcg cgctggacag cgaagatcga
     2821 catgattaca acgctgcctt gcttgtcgcc gccggcggtc atgcaatgca gatttaccgg
     2881 aaactgcacc ttgtcccatt tggcgagtac gttccattac gtcactcgtt tccactcttc
     2941 gccgcgatcg ccagtcgctg ggtgccagcc gatttcgaag tcggccgcga gttcaccgtt
     3001 ttccgcctaa cgaatgcgaa tgtctctgtc gctcccctca tctgcttcga ggacacgatc
     3061 ggcgaattga cccgacgttt cgtccttcgg ggtgcaaacc tgctcgtgaa catgacaaac
     3121 gacgcctggt atctgcagtc agccggttcg cagcaacatc tggcgaatgc cgtctttcgc
     3181 tgtgtcgaaa cgcggcggcc aatgctgcgc gccgccaata ccggcgtgac ctgcttcatc
     3241 aacgaattcg gacgcatcac gcaagtcttg cgcgatgaga atggcggcac atttactgaa
     3301 ggcgtgctga ccggcgaggt gaacgtgccg caggaacctg ccctgagcga cagtcgaacg
     3361 ggtcgacagc taacatttta cacgcgacac ggtgaactct tcgccaaaat ttgcgcaggc
     3421 gtgacggcga tcgcggtcgt tagcgccgta ttgcggcggc ggtggatcgg ccgctccgcg
     3481 ggcgatgtta aataatcgcg gcttcgccgc ctaactttca gcatcgagcg cggagcgctc
     3541 gatccacctc ggtacatgtg ttaaaaacgt gcgacgtggc tgtccaactt ccactcgatc
     3601 acttaccgga gctttggccg aaacaattcc gcggggcgaa aatcggcgcc ttgctgcatc
     3661 cggcgtctgt gtcatccaat ctcgagcacg cctcgcgaat tctcgagaga ctgaacggcg
     3721 atttgttccg gctggtcgcg ttcttcggcc cgcagcatgg ctatctcggg caaacccaag
     3781 acaacatggt cgaatggaaa agctatgagc acccgcgatt gcggattccg gtgcacagct
     3841 tgtacggcga gcatcgggag ccgacgccgg agatattgcg cggcatcgat gttttacttg
     3901 tcgatcttca ggatgtcggc gcgcgttact acacgtttat ctggacgctc tatctctgca
     3961 tgcgggtgtg cgaagcgcat ggggtcgctg tcgtcgtgct cgatcgacct aatccaatca
     4021 aaggcctaac gactgaaggg ccggtgctcg atccagctta ccagtcgttt gtcggcatgc
     4081 atccgatccc ggtgcggcac gggcgaacaa tcggcgagct tgcacagcag tttcgggacg
     4141 aagcgtttcc gaaatgtcga ttgtcgatct tcaggatgtc ggcgcgcg
//
LOCUS       DASTDG010000158        28835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1343569, whole genome shotgun sequence.
ACCESSION   DASTDG010000158 DASTDG010000000
VERSION     DASTDG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 28835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28835
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343569"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFO30_05180"
     CDS             <1..499
                     /locus_tag="VFO30_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418714.1"
                     /translation="LTAMAEHIQRPEVGAVGARLLADEGTVQHAGIILGVNGIADDAC
                     RGFPTENGGARRPAQLTRNCSAVSGACLLTRRNIFEACGGFDEDVRLAPFADVDLCLK
                     IRRAGYLIVYTPFATLRRHKADDRGCDPAAAEVMRQRWGHALQRDPYYNPNLSRARAD
                     FSLGN"
     gene            518..1264
                     /locus_tag="VFO30_05185"
     CDS             518..1264
                     /locus_tag="VFO30_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418715.1"
                     /translation="MNKRDDNLWHGLDAPAREHLTPRDYATGSFAQELSSRGVEAGEL
                     ATADRSRPEIREAWIPGVEIFARKIYPQRHRGMFGEFARCSEGILGRIGLWPKQWAAA
                     RMFAHSAKGFHVHPPSVPSEMRAENWHRRLFIDEPENYALRRYDEEQWDVMFFLQGTA
                     EMILRDTRVGLPPRIMRFFVDGDNHRSRNNVAVVVPPGVAHAIRAEGSEDVIMVYGTT
                     TIFTPEFEGRIASEIETAELPEDWQKFLSL"
     gene            1336..1548
                     /locus_tag="VFO30_05190"
     CDS             1336..1548
                     /locus_tag="VFO30_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418716.1"
                     /translation="MERNRPFVIKMADGNECAVPHRDYIVLAPRAAYVIVFDQKDPHD
                     AFDVLPLLTMTGLRQSGDGVEAGAAE"
     gene            1582..2271
                     /locus_tag="VFO30_05195"
     CDS             1582..2271
                     /locus_tag="VFO30_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9418717.1"
                     /translation="MKRQFDSAEPELMDRPQPVSQELERDLENLCQLNRWFGGHRLIV
                     HFMRRWIQPGARLRIVDVATGAGDIPRLIVDLARKIGATAEIDAIDRQSATLEIARRW
                     SRGYPEISFLEADLLMFNPAAAYDIVLCSLALHHFSDEDAVRVLRRCRELSHKFVLVS
                     DLRRSWFLTVGVYLLTALIFRESMTKYDGRLSAARAFSFHEMDALARQAGWQSFGHKD
                     FRFARQAIWLE"
     gene            2268..3092
                     /gene="panC"
                     /locus_tag="VFO30_05200"
     CDS             2268..3092
                     /gene="panC"
                     /locus_tag="VFO30_05200"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9418718.1"
                     /translation="MRIAARVAEARTRNKSPRVLVATMGALHKAHGELIRVAREHAGN
                     DGEVLVSIFVNPLQFEPGSDYERYPRPQKADEEFCRKRDVDLLFRPTMDEMYAADRSV
                     FVEETSLSSLLEGKSRPGHFRGVCTVVAKLFNILSPDVAVFGEKDFQQLAIVRRMARD
                     LSFNIDIVALPTVRESDGLACSSRNEYLSEEERKQATVLYKALVTAAKADRRSAREIV
                     DLTRGVIGEAPLARIDYVDVVDAETLQPIDSVRPNSLLAVAVYFGKTRLIDNMRLG"
     gene            3089..4687
                     /gene="nadB"
                     /locus_tag="VFO30_05205"
     CDS             3089..4687
                     /gene="nadB"
                     /locus_tag="VFO30_05205"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET9418719.1"
                     /translation="MREYDFVVVGSGIAGLSFALKVARHGSVALITKRKGADTNTAWA
                     QGGIACVTSDEDSFELHVRDTLEAGAGLCDERVVRTIVTEGPARIQELVDLGLHFDER
                     EVSGHREFDLGIEGGHSKRRVLHVRDVTGKEIEDTLLRELHRQADVELLENQMAVDLI
                     TAAKLGFAAEDRCLGLYALDEKSGEVETIRSDRVVLATGGCGKVYLYTTNPDIATGDG
                     VAVAWRAGAVIANMEFIQFHPTCLFHPRAKSFLISEAVRGEGGILRNNRGEDFMKRYD
                     PRGSLAPRDIVARAIDAEIKRSGAQCVFLDITEKSPEFLQGRFPHIYETCLRFGIDMS
                     KQPIPVVPAAHYQCGGIRTDVNGATNLPGLYAIGEVGCTGLHGANRLASNSLLEGLVV
                     AHRAAEACVRARSPSQPGWKMSLPEWESGDAQDVDELVVIYHNWDEIRRLMWDYVGIV
                     RTNKRLQRASARLRNLQREIREFYWNFKVSVDLLELRNLATVAALIVDSALSRKESRG
                     LHYTLDYPHTEDGQFKCETLLSRS"
     gene            4804..5637
                     /locus_tag="VFO30_05210"
     CDS             4804..5637
                     /locus_tag="VFO30_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418720.1"
                     /translation="MKSLALLCLPLVLASTLKADDYSISPYASAADFAKYAMKLREVA
                     LLKVEPQVFVPTASRPTTTRFPWKTNIVTTVFWVGEQAGGNNPVPNFKSSWDADWTSR
                     YGGFDNPDPSARRNYIPVSFVPRQNPFYCALPYNDVTHGQFKPEAPLVIPWFKQAYMG
                     AGQSVCQHRWVAIRKGNRTCYAQWEDCGPFRTDHFQYVFQNERPKPNLNHGAGLDVSP
                     AIRDYLGLAPTDVTDWQFVEVRDVPPGPWRSYGENNNFVIARRELEQQLAKGVSGAAK
                     K"
     gene            complement(5677..7377)
                     /locus_tag="VFO30_05215"
     CDS             complement(5677..7377)
                     /locus_tag="VFO30_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET9418721.1"
                     /translation="MIADSYLQARNELEAAVTGLLKLAGEMRRTPAWLDMLQSFAAEI
                     RQPLLLVIVGESKAGKSSLLNALVDANLIGEAESSRISVFAYGRESKSVDVTPMLREC
                     YMPATTLRDLKIVDTPGIEKLVAQDRQTVLEFVSRADLVLLVLSLRNPWSRASWDFMT
                     GGQLPLKKFVFVLQQADLREPKEIAIIRRNLEDDAKHELGFTPPIFAISARDALRARS
                     IRGQNQYAERQNEFDAFKEQINLVVNQSGGRVQPLRSACQLAQVALHDIASELRASVD
                     AVTRDERRLARTEALRQILKEQTLRTSDDALVRLEGICREQIGGAVEAIKRKLSLANV
                     GSLLIRSSAWLQHCEADFGAKLRASIEQYVEQTAQPLETELRGLWPQLHDTIDDQLIT
                     DAKHKVPHAGPDFAQQKRDLLDAVTRALSQNLTGDLSKIVRLLTKLSGSLRALLVIAG
                     LGAVLAVVTIKFSSGLAAVFALLAVCAASAGAVVAWRGREMILRAYAQAKDVQMRELR
                     NVLTEHFKHAIDAFHDTVLEKLQPLIEHCEVQRRSCEPLLRRAEELQRTLGKLSSRLR
                     "
     gene            complement(7386..8222)
                     /locus_tag="VFO30_05220"
     CDS             complement(7386..8222)
                     /locus_tag="VFO30_05220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9418722.1"
                     /translation="MRSVGWLLLGCIASLALCQLEAENEPSNTRTASAEPRLTFSSVH
                     VAGPYIALTFDDGPHATLTPKLLDLLAAHHIRATFFVIGENVAKHPEIVARAAREGHE
                     IGSHSWSHPNLAKMSDDSVRQQLRQTEDAIENATGRKPTLLRPPYGSVTPRQKRWIHE
                     QFGYEIILWDVDPLDWKRPGPTVVCNRIVKETRAGSIVLAHDIHPGTIAAMPAVLDQL
                     QAKGFQFVTVSELIRMAIPDASRPVKQTVAPATQAQPAQPRAIRQATDSAVRISSPPP
                     NG"
     gene            8421..10319
                     /locus_tag="VFO30_05225"
     CDS             8421..10319
                     /locus_tag="VFO30_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418723.1"
                     /translation="MKILVVEDHLDSRNSLERLLRRRGYEVVGVASAEEARQAITEQS
                     FPFLILDWMLPGKSGLELCKELRAQPGGDDTFILLITARQNTEDLQQALAAGANDYLS
                     KPLDTALLNVRLSVAERQVRELGERKQARAALEESARTMTGILENTTDGFFAVDVSWK
                     FIYLNPEAEKLLGRKRPELLGAELWQQFPELLGSPFESNFRRVIAEQTTMEFEASDVA
                     GKVCFEMRAYPSGTGMSVFFRDVSERKATEEKRLTTSKLESLGTLAGGIAHDLNNILT
                     VISGNIGLAQIEAPAHAPHLLSHLAKAGQAAQHAARLSGQLLTFSKGGSPLKRVTSIA
                     ELLGRAAEFSLYGSNLRAELDVPGTLWKAEVDPAQIEQVINALMINAREAMPHGGVVR
                     LVACNVKVDGRRSALLRPGRYVKIAAIDQGRGVDPKLASKIFDPYFTTKPTASGLGLA
                     ISYSIVKKHGGLLHLESSSPAGSTFAFYLPAAPRVQRNGDHVRLAETGSAFNGQRRVL
                     VMDDEAGIREITAQLLSTLGYQVAAVADGNEAVKLYERGLRRGEQFHAVILDATIRGG
                     MGGVATIEKLRDIDPQVTAIICSGYSDEEALSKFLAYGFSGALPKPFTRRELADVLQR
                     AFQAANTN"
     gene            complement(10355..10819)
                     /locus_tag="VFO30_05230"
     CDS             complement(10355..10819)
                     /locus_tag="VFO30_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9418724.1"
                     /translation="MAEVARVNSRRVILCGAVAVFAFATSAKAGAEWLTDYKKAQEEA
                     KASNRLVLLNFTGSDWCGWCIKLDREIFSTPQFKDYASKNLVLMEADFPRGKPQSASV
                     RKQNEELAMRYQVIGFPTIFVLNGDGKVVGELGYTPGGPSAFIAELEKLRKG"
     gene            complement(10870..11148)
                     /locus_tag="VFO30_05235"
     CDS             complement(10870..11148)
                     /locus_tag="VFO30_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HET9418725.1"
                     /translation="MPKTKTSSKRKPNPAFMRPVQPDDKLAAVVGSKPLPRSELTKKL
                     WDYIKKHGLQDKKKRTQINADDTLRPVFNGKSQVSMFEMTKLVSGHIR"
     gene            complement(11186..12358)
                     /locus_tag="VFO30_05240"
     CDS             complement(11186..12358)
                     /locus_tag="VFO30_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9418726.1"
                     /translation="MAKKADGQILIVDDDRATRRVLVRTLCSAGYDCAEAETGDEALE
                     LVRREQPSLLLLDFEMPGLNGAEVLKKLRADADSDVANVPAIMLTGHTDEQSEVLCLD
                     AGANDFVTKPINTAVLRARIETQLRLRSMREQLQQQNEELEQWRRNLERDLAAARLTQ
                     QSLIPQKRPPLSGWDIADYYRPVIQVGGDIYGWLTLKDARTLFWIVDATGHGASAALL
                     TTLAKLLFHHGSIEHGAPDALMRAVNEDFRSIFGARSFMTAMCVALEAGSGRASVVGA
                     GHPPLLIARQGASAEAVSSHAPPLGVAQPTQFSETVIDLRPGDAFILYTDGLFGGRAR
                     VARRLTPQRLIEKLNGSASDAQTLLEQLLRYSPKSDDDQQLLDDVTAVVVRREVKK"
     gene            12501..12890
                     /locus_tag="VFO30_05245"
     CDS             12501..12890
                     /locus_tag="VFO30_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418727.1"
                     /translation="MKFSLSLALLAAFFISACSDVEEPPARVRRHVAQYPPAGQEQYP
                     PQQQPFNPNAPPPENMPPQEPTAAPPPTAMATRSEKGDHPYGIPVPGKPHVVESPFSP
                     GKYVDVEGFPPGTEVKDPYTGKIFLVP"
     gene            13041..14390
                     /gene="aroA"
                     /locus_tag="VFO30_05250"
     CDS             13041..14390
                     /gene="aroA"
                     /locus_tag="VFO30_05250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9418728.1"
                     /translation="MEFRVKRAPRIDTEITVPGDKSISHRAVIIAALSNGVCTLRGFL
                     LSDDCMRTISALRALGIRIEQPDSDTLVVYGKKRVLTPPKEEIDCGNSATTMRLLAGL
                     LAGQTFESRLVGDAALLRRPMDRIITPLRQMGAAIVAEGPEQTPPIRIHGGSLRGIHY
                     KSPVASAQVKGAVLLAGLFARGKTTFEEPVPTRNHTELMLNYFLVRTAKENDTSVSIF
                     GDQMPESRDFDIPGDISSAAFWLVAAAAQPGGHLLVRNVGLNETRTAALGVLVRMGAQ
                     VREAIEDVDQVEPRGIVEVTGAPLKGTVIQGKEIPQLIDELPVLAIAGALASGTTIIR
                     QAHELRVKETDRLAAIAHNLRLMGAQVLEMNDGLEIHGPTPLRGARVPSFGDHRIAMA
                     FAVAGLFADGDTVVQDADCVRESYPEFASTLEEFTNQKRMRTSTPVIGSLAARLGEE"
     gene            14450..15103
                     /gene="cmk"
                     /locus_tag="VFO30_05255"
     CDS             14450..15103
                     /gene="cmk"
                     /locus_tag="VFO30_05255"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9418729.1"
                     /translation="MQTSCHWVVTIDGPAASGKSSVARELARRLRFVYVNSGAIYRAI
                     TWYVLRQGVPASDSARIAQLVGAAAIDCQLENNESRVVIGAIDPTPHLREPSVNDAVS
                     QVSTVSAVRHIVTEKVRGYAREHDLVVEGRDIGSVVFPDTPFKFYIDASPDVRLRRRA
                     AEGQRDEIAARDWVDSSRRSAPLTIAPNAHVIDSSELSVTDVVDEIVRRLRETNLPI"
     gene            15157..15843
                     /locus_tag="VFO30_05260"
     CDS             15157..15843
                     /locus_tag="VFO30_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9418730.1"
                     /translation="MNFYYWIGYHGSRLLAQLFFRFRIIHRERMIQTGPVIVAMNHQS
                     YLDPPLAGNACDRAIYFLAKKSLLDAPILRWLLPKLNVIPVNLEGNDRRALKTLIRVL
                     RAGECVLVFPEGSRTPDGNLQPALPGVGLLIAKTRAPVVPMRIFGAFDAWPIGGKLRL
                     WRRITIIVGEPIYFSAADFQNPGRTTCTAVSQRVMDAIGGIKMPEAKIDIRRTDNDVK
                     PATSTSIIRR"
     gene            15840..16019
                     /locus_tag="VFO30_05265"
     CDS             15840..16019
                     /locus_tag="VFO30_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418731.1"
                     /translation="MNPPPTSRVCKDAIVVMIIIVAVMAFVAIYANVQRWRRDKLEHV
                     IITPVASPTASPAAP"
     gene            16061..17821
                     /locus_tag="VFO30_05270"
     CDS             16061..17821
                     /locus_tag="VFO30_05270"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET9418732.1"
                     /translation="MNRFLIFSAAGLLASFIGCQKQQSEAERNAEVERQVNERLAAEH
                     QAQEQQRLVQRQDELDARLRELNEQQRAAASTPEPQRTETERSTRETFEAQPAASYSM
                     FYTKLEPFGVWRETPTYGYVWQPREAMQSRSWRPYTNGHWIYTDAGWTWISEEPFGWA
                     AYHYGRWMRLRGIGWVWVPGSEWAPAWVSWRTSNDYVGWAPLPPEARFERDAGIKNWA
                     DGYYDIGPEQYCFVPTREIGTQRIERVVVPPEQNVTIVNQTTNVTNITYNNSMIVNQG
                     PNYDELRARSQQPIQHLRLERRTTVEMENPRPIVKGEVVEMPAPVIAATQPVERPATV
                     RETVAAVVERGWEGIADQQAVQRARAKIKSEATPPPNAPPKTFIKPAPATGQTRATVP
                     SPRPIATAIARSTITPPATSVVQTPTPTQAVRPTQTPIPSRAAAPSAPPTEGAVHTAT
                     PIATPTPAMLPTRLATATPRPTATAVATPTAIARPTFSPRPSATAAFSPTPAASPTFG
                     RAGTKEQREAQKELRKQERAARKQERLERATPTPAATATASVPASASPGAAESPGKHK
                     KRPGEAATSPTATPTASPSR"
     gene            complement(17793..20519)
                     /locus_tag="VFO30_05275"
     CDS             complement(17793..20519)
                     /locus_tag="VFO30_05275"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9418733.1"
                     /translation="MDVRYERGVYLPQAHLWLDPWDAKPLAFVSHAHSDHIAPHQEII
                     ASPGTARLLRARMPGERQEHVLPFGVKRRFHGVDLMLLPAGHVFGSAQLYLETANESL
                     LYTGDFKLRRGKSAELAEWKHAATLIMETTFGQPRYRFPPTEQVVDQIVAFCREALDD
                     GVVPVLFGYSLGKAQEILCALEGAGLVPMLHGSVFQITHVYEQLGRSFCKYVRYNPND
                     LAGKVLICPPSANHSRMIDNIPRKRTAMISGWAVDANAVYRYQVDAAFPLSDHADYDD
                     LIRYVHLVQPKRVLTLHGFAAEFARDLRARGMDAWALGEENQIEMNLDAGPVAPAVPG
                     GNAHAVETTATIESEFGEFARIGEAIAATPPKLEKVRVLSDYLRSLSPAQLAIATTYF
                     TGRAFAQSDPRTLQVGWSVIFRALQAATKISDSEFHRIAAKHGDAGKAAFEILASRTQ
                     PEPFSISESRKLFENLHRARGPIAKAELLEKRMSILSAKEGEYVVKILTGDLRIGLRE
                     GLVEEAVAKAFDVPLEDVKEANMLLGDIGATALLASKKELDRAELSIFRPIKCMLATP
                     EPTAEAIWARFEHAKVVYAEDKFDGIRAQLHHSAQRAEIFSRDLKQITEQFPELANTA
                     RQFDVDLILDGEIVAFEHGRKLTFFDLQKRLGRRSDGADLFDAASADVPVAFIAFDLL
                     WLSGRSLLKTPLRERRELLRSVRLPPRFEAAETFPAHSAAELEEIFQQARRRANEGLM
                     VKDPESFYMPGRSGMFWYKLKKELATLDVVVVAAELGHGKRNHVLSDYTFAVREETTG
                     ELLPIGKAYSGLTDAEIAELTEHFKQNTIADHGRYREVKPDIVLEVAFNSIQPSTRHT
                     SGLALRFPRIKAIRRDKTADAIDTLAYARELAAQQSRSSAENTPNEKAKPSA"
     gene            20615..21202
                     /locus_tag="VFO30_05280"
     CDS             20615..21202
                     /locus_tag="VFO30_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418734.1"
                     /translation="MKTVSLALLLGFSLPTALRAESQPEPKLLAKVTPLPVALSDDFD
                     FRKVKLYFLSEKPTQLKTRRSMSLNPNAYATDTGQQAVVNQDSSLSFERQYRLFGAVT
                     ALDQRQRLGDYLDFFWRAKRPADVTVRLEYRQEKLHDLVQAQEVSYTNVHGTHKTEFR
                     VIGDDYLDDGRVTAWRCLLIENGRIVAVRRSYLWD"
     gene            21275..21790
                     /locus_tag="VFO30_05285"
     CDS             21275..21790
                     /locus_tag="VFO30_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9418735.1"
                     /translation="MESSILVGVSGPAVWIKVEGKGSFLNSGNLKEFAKQMVDRGYRQ
                     FIVDLADCAMMDSTFMGTMAGIALRLKELGRGHLHIVHCGNRSHELLAGLGLDHVFDI
                     HTNGSVAPKCETLSRQASGESVIEQKQERAQTMLQAHEALCQVAPENVFRFKDVLDYL
                     KQDLHQKTASK"
     gene            21805..23244
                     /locus_tag="VFO30_05290"
     CDS             21805..23244
                     /locus_tag="VFO30_05290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET9418736.1"
                     /translation="MSPSLLFGLLLLCIAGWIYTTLLLSRRLRVLERSKEEIQVEETL
                     VFDFLHGLGEAFSETIRPNHLHRLIVEGATRVLDAHGGALYMVDRTGTKLLPAFVSKG
                     CPPLVEIPEDVLAQAAASPAALESYVRLRAVGAGEGTIGRVWQMGQVIYLNELSQAPE
                     LAKLRDSSFGAASVMMGALLYAKQNMGVLAVANGPMGSPFSQSDFVVFKSIAEQSAFA
                     LYNAIIYSEANEKKRLDHDLEIARDIQRILLPAEAPAVNGFELNGINVPARQVSGDYF
                     DYIKVDDERLGVAIADVSGKGVPASLIMAICRSVLRSQAAQNPSPADVLQKVNRQLYP
                     DIKEDMFISMAYLVLDHAHNGTVTLARAGHDAPLLYKRSAQSVTRLKPPGMVLGIDSG
                     SVFDRLTNDVAVPLERDDCLVLYTDGVTEAIDGEGNEFGLERMMESVRASATNGAAAI
                     VTRLIDDLRSFVGARPQNDDVTLIAIRKT"
     gene            23241..23858
                     /gene="grpE"
                     /locus_tag="VFO30_05295"
     CDS             23241..23858
                     /gene="grpE"
                     /locus_tag="VFO30_05295"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9418737.1"
                     /translation="MKKIPATDSGADKKQNGAPASAAANATSTLHEEKPTESSATAQS
                     ETEDPMAGLQADLDRFRDLALRSQADFENYKKRCAREKEEAVKYANSSLLERLVAIVD
                     NFELGLNAAREQSMDSPIYSGMTLVLKQLNDFLAENGLQPIEAVGEKFDPNRHEAIAH
                     EPNSDAPEGTVIRQVRRGYRFRDRLLRPATVVVSAGPAKHRTGAE"
     gene            23919..25046
                     /gene="dnaJ"
                     /locus_tag="VFO30_05300"
     CDS             23919..25046
                     /gene="dnaJ"
                     /locus_tag="VFO30_05300"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9418738.1"
                     /translation="MATKKRDYYEVLGVGRNSSEDEIKRAYRKLAVKFHPDKNPDDPH
                     AEEKFKELGEAYDVLMDADKRAAYDRFGHAAFAQGGVGAGFHDPFDIFREVFAGAGGG
                     IFETFFGGGTMRAEDRQRGSDLRYDMQITLEEAAAGVEKEIEIRKLDTCDKCHGSGAE
                     PGSRTITCPTCGGRGQVISSRGFFQVSQTCPRCRGIGHIIDKPCRHCHGDGRVEKLGR
                     IKLKIPTGIADGSRLRSSRNGEAGTRGGPHGDLYVVVHVKEHKIFHREDDDLYCELPI
                     PFTVAALGGEVEVPTLEGKAHLKVPSGTQSGQIFRLRGKGIVNVNGRERGDLLARLMV
                     EVPTRLNNEQRQKLEEFGALCGDENTPIRKSFFERAKEFFG"
     gene            25109..25861
                     /locus_tag="VFO30_05305"
     CDS             25109..25861
                     /locus_tag="VFO30_05305"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET9418739.1"
                     /translation="MHRFYISPQQWNPDLLVLTGAQAHHARDVLRLGRDDKVAVFNGR
                     GREITAKIVDVSGREVSLAKLTQSETPPLRSRILLGQAIPKGKNMDLIVQKAVELGVA
                     EIFPLISARTIVDLAQDEARKKQAKWQTVAIEAAKQSGQNWLPQIHLPQTMKQFFAEL
                     PPSDLQLIGSLQPGALHLKKILAEFAAEHRCRPHSVLMLIGPEGDFTPAELALARSHA
                     CRPITLGPIILRVETAAIYCLSVLSYELFAPQ"
     gene            complement(25855..26211)
                     /locus_tag="VFO30_05310"
     CDS             complement(25855..26211)
                     /locus_tag="VFO30_05310"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET9418740.1"
                     /translation="MGENLARRFLRKNGYKILYRNFRGRSGGEIDIVARDKDTLVFIE
                     VKTRTHEEFGRPLSAVDRQKRKRISRGGLAWLRLLDNADVLFRFDVVEILVRDDAEPR
                     IELVKNAFQLPAPYLY"
     gene            complement(26165..26872)
                     /locus_tag="VFO30_05315"
     CDS             complement(26165..26872)
                     /locus_tag="VFO30_05315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9418741.1"
                     /translation="MYRRCGFRYEKNLRAAGVAHIAGIDEAGRGALAGPVVAAAVILP
                     EKFRHRKLNDSKQLAPEVREKVYADVTTNAEVIWATGVVDHIEIDAINILRATHKAMR
                     AALAALALQPDHVLIDGLPVFPFDFPQTAIIDGDCVSKTIAAASVIAKVTRDRLMRDF
                     CAQFPEYCFSQHKGYTTELHLTKLHEFGPCPIHRRSFEPVAQPLLALEEFVPAGTHSA
                     RRIGGESGAALPPEERL"
     gene            complement(26984..27325)
                     /gene="rplS"
                     /locus_tag="VFO30_05320"
     CDS             complement(26984..27325)
                     /gene="rplS"
                     /locus_tag="VFO30_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9418742.1"
                     /translation="MNRLIDAIEKEQRKADGTQFSVGDSVHVHTRVVEGDKERIQIFS
                     GIVIGRKGRGLNQTFTVRRISYGEGVERVFPLHSPRIGKIEVEKQGRARRAKLNYLRT
                     RKGKEATAVRE"
     gene            27537..27610
                     /locus_tag="VFO30_05325"
     tRNA            27537..27610
                     /locus_tag="VFO30_05325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27571..27573,aa:Arg,seq:ccg)
     gene            complement(27725..28054)
                     /gene="trxA"
                     /locus_tag="VFO30_05330"
     CDS             complement(27725..28054)
                     /gene="trxA"
                     /locus_tag="VFO30_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9418743.1"
                     /translation="MEPSAAITVDESTFDREVTQSDKPVIVDFWAEWCGPCKMIAPVL
                     DEIAREKAGAVKVAKVNVDENQSLSFKYNIRAIPALLFFKNGQLRDQVTGVTSKKDLL
                     ARLDTLG"
     gene            complement(28058..28492)
                     /locus_tag="VFO30_05335"
     CDS             complement(28058..28492)
                     /locus_tag="VFO30_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418744.1"
                     /translation="MAEPNQPKRETVRIVAPTKTGDSEAPAQSERINLPARPPVNMPP
                     PPNPPPTENVAPPASPHEPPRKETARIPLPTEPPSPAPAGEMKKTQPLFTMPEPAEAA
                     PTPLMVSGDTEARVETIPAALCWTILAVSAVLLILQIWNYFS"
     gene            complement(28554..>28835)
                     /locus_tag="VFO30_05340"
     CDS             complement(28554..>28835)
                     /locus_tag="VFO30_05340"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9418745.1"
                     /translation="EGSRAATLPDQISARIKNARHRVAMSLQQKIAYELARERIGHKL
                     KLLVDEPHIARTEADAPDVDARVILSEAAVPGEFVWRTIGGSRGYDLLA"
BASE COUNT         5919 a         8370 c         8581 g         5965 t
ORIGIN      
        1 gctcacggcg atggctgagc atattcaacg gccggaagtt ggcgcagtcg gggcgcgcct
       61 gcttgctgac gaaggaacgg tgcagcacgc tggcatcatc ctgggcgtca atgggatcgc
      121 agacgacgct tgtcgcggat ttccgacgga aaatggtggt gcaagacggc cggcgcaatt
      181 gacccgcaac tgcagcgccg tcagcggggc gtgcctgttg acccgtcgta acattttcga
      241 agcatgcggc ggattcgatg aagacgtgcg cctcgcgcct tttgctgatg tcgatctttg
      301 tctcaagatt cgtcgtgccg gctatctgat cgtttacacc ccgttcgcga ccttgcggcg
      361 gcacaaagca gacgaccgcg gctgcgatcc ggcagcggcc gaagtgatgc ggcaacgctg
      421 gggtcacgct ctacaacggg atccgtatta caacccaaat ctttctcgcg cccgtgccga
      481 tttctcgtta ggcaactgac ttgcttccaa aatcgacatg aacaagcgcg acgacaatct
      541 ctggcatggc ttggatgcac cggcacgcga gcacttaacg ccgcgcgatt acgccaccgg
      601 ctcgtttgca caggaacttt ccagccgtgg tgtcgaggcg ggtgagctcg caactgcgga
      661 ccgctcgaga ccagaaataa gggaggcgtg gattcccggc gtcgaaattt tcgcgcgcaa
      721 aatttacccg cagcgccatc gcggaatgtt cggcgaattt gcgcgttgca gcgaaggcat
      781 tttgggcaga attggccttt ggccaaagca atgggcggcg gcgcggatgt ttgcgcatag
      841 tgcgaaggga tttcacgtcc atccgccaag cgttccgtcc gaaatgcgcg cggagaactg
      901 gcatcgccgt ttgttcatcg acgagccgga gaattatgcg ttgcgccgct atgacgagga
      961 gcaatgggac gtgatgttct ttttgcaggg caccgcggaa atgatcctgc gcgacacgcg
     1021 agtaggtttg ccgccgcgca tcatgcgctt ttttgtcgac ggcgacaatc accgcagccg
     1081 caacaacgtc gccgtggtcg tcccgccagg ggtcgcgcat gccattcgag cagagggttc
     1141 cgaagacgtg atcatggttt acggcacgac cacgatcttc acgccggagt ttgagggccg
     1201 catcgccagc gaaatcgaga ctgcggagct gccggaagat tggcaaaaat ttctttcttt
     1261 atagaccagc tgtttgaccg gagataagaa aatcgacatt ctgctaatgt gacgcgcagg
     1321 caaatagaga aggcgatgga gcgaaatcgc ccgtttgtca ttaaaatggc cgatggcaac
     1381 gaatgcgcag tgcctcatcg cgattacatc gtcctcgcgc cacgcgccgc ctatgtgatc
     1441 gtattcgatc agaaggatcc gcatgacgca tttgatgttt tgccgctgtt gacaatgact
     1501 ggactgcggc agtctggcga cggagtcgaa gctggcgcgg cggagtagtg cgattgccag
     1561 ataacgccgc taccgtcggg tatgaagcgg caatttgatt cggccgagcc ggaactgatg
     1621 gatcggcccc agccggtctc gcaagaactg gagcgcgacc tcgaaaacct gtgtcagctc
     1681 aatcgctggt tcggcggtca ccggctcatc gtgcatttca tgcgtcgctg gattcaaccg
     1741 ggcgcgcggc tgcgcattgt cgacgttgcc accggcgcgg gcgacattcc gcggctcatt
     1801 gtcgatcttg cgcgaaagat cggggcgact gctgagatcg acgcgataga tcgacaatcc
     1861 gccacgctcg agatcgcacg ccgctggagc cgtggctacc ccgaaatctc gtttctcgaa
     1921 gctgatcttc tgatgtttaa tccagccgcg gcttacgaca ttgtgctgtg ctcgctcgcg
     1981 ctccatcact tcagcgatga ggacgcggtg cgggtgctac gacgctgtcg cgagttgtca
     2041 cacaagttcg ttctcgtttc cgatctgcgc cgcagctggt ttcttaccgt tggcgtgtat
     2101 cttttgactg ccctgatctt ccgcgagtcg atgacgaaat acgacggccg cctctccgcc
     2161 gcgcgcgcat tttcgtttca cgaaatggat gcactggcgc gccaagccgg ctggcaaagc
     2221 ttcggccaca aagacttccg tttcgcccgc caggcgatct ggcttgaatg agaattgcgg
     2281 ccagagtcgc agaggcgaga acccgcaaca agtcgccgcg ggtgctggtg gcgacgatgg
     2341 gggcgttgca caaggcgcac ggcgagttga ttcgcgttgc gcgagagcat gcggggaatg
     2401 acggcgaagt gctggttagt attttcgtta acccgctgca gttcgagccg gggagcgatt
     2461 acgagcgcta tccgcggccg cagaaagctg acgaagagtt ctgccgaaag cgtgatgtcg
     2521 atcttttatt tcggccgacg atggacgaaa tgtacgcggc cgatcgatcg gtattcgtgg
     2581 aggagacgtc gctctcgagc ctgctcgaag gaaaatcgcg gcctggacat ttccgcgggg
     2641 tttgcaccgt cgtggccaag cttttcaaca tcctttcgcc cgatgtcgcg gtctttggcg
     2701 aaaaagattt ccagcaactg gcgattgtcc ggcgaatggc gcgcgacctc agtttcaaca
     2761 tcgacatcgt tgcgttaccg acggtgcgcg aatccgacgg gctggcgtgc agttcgcgca
     2821 acgagtattt gagcgaggag gagcgaaaac aggcgacggt tttgtacaag gcgctggtga
     2881 ctgcggcaaa agccgatcgc aggtcggcgc gagagattgt cgatcttacg cgaggcgtga
     2941 tcggcgaagc gccgctggcg cgaattgatt atgtcgatgt ggttgacgcg gagacgttgc
     3001 agcccatcga ttcggtgcgg cctaactcgc tgctcgcagt cgcggtttac tttggtaaaa
     3061 ctcgtttgat cgacaatatg cgactgggat gagggagtat gatttcgtcg tcgtcggcag
     3121 cggcatcgcg ggcttgagct ttgctttaaa ggtagcaagg catggctcgg tcgcgctgat
     3181 cacgaaacgg aaaggcgccg atacgaacac ggcctgggcg cagggcggca tcgcgtgtgt
     3241 gacgagcgac gaagattcat tcgagctgca tgtgcgcgac acgctcgagg ccggcgccgg
     3301 cctttgcgac gagcgggtgg tgcgcacgat cgtgacggag ggtccagccc gcatccagga
     3361 acttgtcgat ctcgggttgc atttcgacga gcgcgaagtc tccggccatc gcgagttcga
     3421 tctcggtatt gagggcggcc attcgaaacg gcgcgtctta catgtgcgtg acgtcaccgg
     3481 caaggaaatc gaggacacat tgctgcgcga attgcatcgc caagccgatg tcgagcttct
     3541 cgagaaccag atggccgtgg atttgatcac cgcggccaag cttgggtttg cggccgagga
     3601 ccggtgcctg ggtctctatg ctctcgatga gaaaagcggc gaggtcgaaa cgatccgttc
     3661 cgatcgcgtc gtgctggcga caggcggttg cgggaaagtt tatttataca cgacgaaccc
     3721 ggacatcgcc acgggtgacg gcgtggcggt ggcgtggcgc gccggcgcgg tgatcgcgaa
     3781 catggagttc attcagttcc acccaacctg tctctttcat cccagagcaa agtcgttcct
     3841 gatctcggag gcggtgcggg gcgaaggcgg gattttgcgc aacaaccgcg gcgaagattt
     3901 catgaaacgc tacgatccac gcgggtcgct tgcgccgcgg gacatcgtcg cgcgcgcgat
     3961 cgacgctgag atcaaacgct ccggggcgca atgcgtgttt ctggatatca ccgaaaaatc
     4021 gcctgagttc ttgcaggggc gcttcccgca catttacgag acgtgtctgc gctttggcat
     4081 cgacatgtcg aagcagccga tcccggtcgt cccagcggca cattatcagt gcggcggaat
     4141 cagaaccgac gtcaacggcg cgacgaactt gcccggtttg tacgcgatcg gcgaagtcgg
     4201 ctgcaccggg ttgcacggcg cgaatcggct ggcgagcaat tcgctgctgg aggggctggt
     4261 cgtggcgcat cgcgcggccg aagcttgcgt gcgcgcgcgg tcaccgtcgc agccgggatg
     4321 gaagatgtcg ctaccggaat gggaatccgg cgacgcccag gacgtggatg agctggtcgt
     4381 gatctatcac aactgggacg agatccggcg attgatgtgg gattacgtcg gaattgtgcg
     4441 cacgaataag cgcttgcagc gcgcgagcgc acggctgcgc aatcttcagc gtgagatccg
     4501 cgagttttac tggaacttca aagtgtcagt agacctgctg gagcttcgaa acctcgccac
     4561 ggtggcggcg ctcatcgtgg actccgcgct gagccgaaaa gaaagccgcg gtttgcatta
     4621 cacgcttgat tatccgcaca ccgaagatgg acaatttaaa tgcgaaacgc tgctgagccg
     4681 gagctaacac tggaacgctt agtgctccac tattcgttag ccgcctcagg gcgaagattt
     4741 tgtagcgtgg aaaactgttg ctcgatcttt ataaatctga taaaaaggtc aggcgtttcc
     4801 ctgatgaaga gccttgccct gctttgcttg ccacttgtcc tggcgagtac attaaaagct
     4861 gacgactact cgatttcgcc ttacgcgagc gcggccgact tcgccaagta cgcgatgaag
     4921 ctgcgcgagg tggcgttgct gaaagtcgaa ccgcaagtat tcgtgccaac tgcctcccga
     4981 ccgaccacga cccgcttccc ctggaagacg aacattgtga cgacggtgtt ttgggtcggc
     5041 gaacaggccg gcggtaacaa cccggtgccg aatttcaaaa gctcctggga cgcggattgg
     5101 acgagcaggt acggcgggtt cgacaatccc gacccgtccg cacgccgcaa ttacattccg
     5161 gtttcattcg tgccgcgaca aaatccgttc tattgcgcac tgccttacaa cgatgttacg
     5221 cacggccagt tcaagccgga agcaccgctg gtcattccgt ggttcaaaca ggcgtacatg
     5281 ggggcgggcc agtcagtttg ccaacatcgt tgggtggcaa ttcgtaaggg caatcggacg
     5341 tgctacgcgc agtgggaaga ctgcgggccg tttcgcactg atcatttcca gtacgttttc
     5401 cagaatgagc ggccaaagcc gaacctgaac cacggggccg ggttggacgt ttcgcccgct
     5461 attcgcgatt atctcggtct tgcgccaacc gatgtgacgg attggcagtt tgtcgaggtt
     5521 cgcgatgtgc caccggggcc atggcggagt tacggcgaga acaacaactt cgttatcgct
     5581 cggcgtgagc tcgaacagca actcgcaaaa ggcgtttcgg gcgcagccaa gaaatagggt
     5641 tgttagccgc ggcgagtccg tcctgcggaa tccaatttaa cgcaggcgcg atgacaactt
     5701 gccaagagtg cgttgcagtt cttccgcgcg acgcaatagc ggttcgcagc tgcgccgttg
     5761 cacttcgcaa tgctcgatga gcggctggag cttctccagc accgtatcgt gaaacgcatc
     5821 aatcgcgtgc ttgaagtgtt cggtgaggac gtttcgtagc tcgcgcatct gcacatcttt
     5881 tgcttgcgcg taggctcgca aaatcatttc gcggccgcgc caggccacca cagctccagc
     5941 agatgcggca cacaccgcaa gcaaggcgaa aacggcggcg agaccgctac tgaattttat
     6001 tgtaacgact gcgagcacgg cgcctagccc tgcgatcacg agcaacgccc gcaggcttcc
     6061 cgaaagtttc gtgagcaatc gaacgatctt tgagagatcg ccggtgaggt tttgggacag
     6121 tgcacgcgtt accgcatcga gcaagtcgcg tttctgttgt gcaaaatcgg gacctgcgtg
     6181 cggcactttg tgtttggcat cggtaatgag ttggtcgtcg atggtgtcgt gcagttgcgg
     6241 ccaaagtcca cgcagctcgg tttcgagcgg ctgtgcagtc tgctcgacgt actgttcgat
     6301 cgatgcgcga agcttcgctc cgaaatcagc ttcgcaatgc tgcagccacg cgctggaacg
     6361 gataagcagg ctgccaacgt tcgcaagtga gagcttccgc ttgatcgctt cgactgctcc
     6421 gccgatctgt tcccggcaaa ttccctccag cctaacgagt gcatcatcgc tcgtccgcaa
     6481 cgtttgttcc tttagtattt gccgcagtgc ttcggtccgt gccagccggc gctcgtcgcg
     6541 agtgactgcg tctacggacg cgcgcagttc gctcgcgata tcatgcagcg ctacctgcgc
     6601 aagctggcag gccgaacgca gcggttgcac gcgtccgcca gattgattga caaccaggtt
     6661 tatctgctct ttgaaggcgt cgaactcgtt ttgtcgctcc gcgtattggt tctggccgcg
     6721 gatcgatcgc gcgcgcagcg cgtcgcgcgc agagatcgcg aagattggcg gcgtaaatcc
     6781 aagctcgtgc ttcgcatcgt cctcaagatt gcgccgaatg atcgcgatct ctttcggttc
     6841 gcgaaggtct gcctgttgca aaacgaagac gaactttttc agcggcagtt gaccgccggt
     6901 catgaaatcc cagcttgccc gcgaccacgg attccgcagc gacaacacaa gcaggacgag
     6961 gtcggcgcgc gagacaaatt caaggactgt ttgtcgatct tgcgcgacta atttctcgat
     7021 acccggcgtg tcgacgatct tgagatcgcg cagcgttgtg gctggcatat agcattcccg
     7081 cagcattggt gtcacgtcga cagacttcga ttcgcggccg tatgcgaaga cggaaatgcg
     7141 gctcgattcc gcctcgccga tcaaattcgc gtcgaccagt gcgttgagca gtgatgactt
     7201 tccggctttc gattctccga caatcacaag caggagcggc tggcgaattt ccgcggcgaa
     7261 gctctgcagc atgtctaacc acgcaggcgt gcgccgcatt tcgccggcga gcttcagcag
     7321 acccgtgacg gctgcttcca actcattgcg cgcctgcagg taggagtcgg cgatcatgcc
     7381 gcgacttaac cgttcggtgg tggcgaagag attcgcaccg ctgaatccgt cgcttggcgg
     7441 atggccctcg gttgcgccgg ctgcgcttga gttgcaggcg caacagtttg tttgacaggc
     7501 cgcgacgcgt ccggaatcgc catacgaatc agttccgata cagtgacgaa ctgaaatcct
     7561 tttgcctgca gttgatccag aaccgcaggc atcgcggcaa ttgtccccgg gtgaatgtcg
     7621 tgcgccaaaa caatggagcc ggcgcgcgtc tctttgacaa tgcggttgca aaccacggtc
     7681 gggccaggcc gtttccaatc caatgggtca acgtcccaga gaatgatttc gtagccgaat
     7741 tgttcgtgaa tccagcgctt ttgccgcggg gtcaccgatc catagggcgg gcgcagtaag
     7801 gtcggcttgc gtccggtggc gttttcgatc gcgtcttcag tctggcgtaa ttgctgccgc
     7861 acgctgtcat cggacatttt cgccaaattt gggtgtgacc aactgtggct gccgatctcg
     7921 tggccttcgc gcgccgcgcg cgccacgatc tccggatgct tagcgacgtt ttcgccaata
     7981 acaaaaaacg tcgctctgat gtgatgggcg gcgagaagat cgagcagctt cggcgtgagg
     8041 gtggcgtgcg ggccgtcatc aaacgtgagt gcgatgtacg ggccagcgac gtggacggaa
     8101 ctgaaagtga gccgcggttc agcggacgca gttcgggtat tgctcggctc gttctccgcc
     8161 tctaattggc acagcgccaa tgaggctatg caaccgagaa gcaaccaacc gacggaacgc
     8221 atgaaaagga gagcggagcg gatgcgtgta tctagcagag gctttcacgg gtgcaacgcc
     8281 gcgtccgaca cgcgcgcaag atcgacaaat cgccgagcgg ctggcagtca tcctgctctt
     8341 ttcatgagcg aacgggtcgg gctgcgcgct cagctgtgca caaggtgagt tgaggggcgg
     8401 gtggaagaga atgctcccga atgaagattt tagttgttga agatcatctg gacagtcgca
     8461 acagcctcga acggctgttg cgtcgacgcg gttacgaagt ggtcggcgtc gcgagcgccg
     8521 aagaagctag gcaagcaatc acggaacaga gttttccgtt cctgattttg gactggatgc
     8581 tgcctgggaa aagcgggctt gagctgtgca aggagctgcg ggcgcagccc ggtggggacg
     8641 acacgttcat cctcctcatc accgcgcggc aaaataccga agacctgcag caggctctcg
     8701 ccgcgggggc aaatgattat ttgagcaaac cgctcgatac ggccctgttg aatgtgcgct
     8761 tgtcggttgc ggaacggcag gtccgtgagc tgggcgagcg caagcaggca cgtgccgcgt
     8821 tggaggaatc ggcacgaacg atgaccggca tactggaaaa caccaccgac ggatttttcg
     8881 ccgtcgatgt gagctggaaa ttcatctatc tcaatccgga agcggagaaa cttctcggtc
     8941 gcaaacggcc tgagttgctc ggtgccgagc tgtggcaaca atttcccgaa ctgctgggct
     9001 cgccgttcga gtcgaatttc cgccgggtca tcgctgagca aacgaccatg gaattcgaag
     9061 ccagcgacgt agccggcaaa gtgtgctttg aaatgcgcgc ttatccgagc gggacgggca
     9121 tgtcggtttt cttccgcgac gtcagtgaac gcaaagcgac ggaagagaag cggctgacga
     9181 cgagcaaact cgaatcgctc ggcactttgg ccggcggaat tgcgcacgac ctcaacaaca
     9241 tcctcaccgt tatttcgggc aacatcggtt tggcgcagat tgaagccccg gcgcatgcgc
     9301 cacatctact ttcacatctg gcaaaggccg gacaggccgc gcaacacgcg gcgcgccttt
     9361 ccggccaatt actaacattc tctaaaggcg gttcaccact caaacgtgtc acctcgattg
     9421 ctgagttact cggacgcgcc gctgaatttt cgctctacgg ttcgaacttg cgcgcggaac
     9481 ttgacgtgcc gggtacgctg tggaaagccg aagtcgaccc ggcgcaaatc gagcaggtca
     9541 taaatgcgct catgatcaat gcgcgcgagg cgatgccgca tggcggcgtg gtccgactgg
     9601 tggcgtgcaa tgtaaaagtg gacggaagac gcagtgcgct cttgcggccg ggccgctacg
     9661 tcaaaatcgc ggctatcgat caaggccgcg gagtcgaccc gaaactcgca tcaaaaatct
     9721 tcgatcctta tttcacaacg aaaccaacgg cgagcggcct cgggttggcg atcagctact
     9781 cgattgtcaa aaagcacggc ggcttgttac atctggaaag ttcttcgcct gcaggatcga
     9841 catttgcatt ttacctgccg gcagcgccac gggtgcaacg taacggtgat cacgtccggc
     9901 tcgccgaaac cggctcagca ttcaacggcc agcgtcgggt cctggtgatg gacgacgaag
     9961 cggggatccg cgagattaca gcgcagttgc tcagcacgct cggctaccaa gttgccgccg
    10021 tggccgacgg taacgaggcg gttaaacttt atgaacgcgg gctgcggcgg ggcgaacaat
    10081 ttcacgccgt cattctcgac gcaacgatcc gcggcggaat gggtggcgtg gcgacgatcg
    10141 aaaagctgcg cgacatcgac ccgcaagtga ccgctatcat ttgcagcggc tactcggatg
    10201 aagaggcgct ttcgaagttt cttgcctacg gtttctctgg cgcattgcca aaaccgttca
    10261 cgcgtcgcga gctggcagac gttcttcagc gcgcctttca agcagcgaac acgaattgac
    10321 cgtcggcaaa caacgacgac ctcgaaaaga tcggtcagcc cttgcgcagt ttctctaact
    10381 cggcgatgaa cgcgctcggc ccgccgggag tataaccaag ctcgccaacc actttcccat
    10441 caccgttcaa cacgaaaatg gtggggaaac cgatgacctg atatcgcatc gcgagttcct
    10501 cgttctgttt tctgacgctg gccgactgcg gcttgccgcg cggaaaatct gcctccatca
    10561 ggaccagatt cttactcgcg taatctttga actgcggcgt cgaaaagatt tcccggtcga
    10621 gcttgataca ccagccgcac cagtcggacc cggtgaagtt aagcagcacc aatctgttgc
    10681 tcgctttcgc ttcctcttgc gcttttttgt aatcggtgag ccactccgcg cccgccttcg
    10741 ccgatgtggc aaaggcaaaa acggcaacgg cgccgcaaag aattacccga cgactgttca
    10801 cgcgggcaac ttcggccacg cgagcgcgaa ctgcaacaac acgagttgcg cgataatccg
    10861 cgcggaaaac taccggatgt ggccggagac gagcttggtc atctcgaaca tgctgacctg
    10921 actcttgccg ttaaataccg gccggagcgt gtcgtccgcg ttgatctgcg ttctcttctt
    10981 tttgtcctgc aggccgtgtt tcttgatgta gtcccaaagc tttttcgtga gctcggagcg
    11041 cggcagcggt ttggaaccaa caacggcagc gagtttatcg tcgggttgca ccggcctcat
    11101 gaaggctgga tttggcttgc gtttggaaga agttttcgtt ttcggcatac agggtgatta
    11161 ctgcctcgcc cgttgcaggg caacactatt tttttacttc tcgccgaact acgactgccg
    11221 tcacatcgtc gagcaactgc tggtcatcgt ctgatttcgg ggagtaacgc aacagttgct
    11281 cgagcagtgt ctgagcgtct gatgccgaac cattcagttt ttcgatcaac cgttgcggcg
    11341 tcagccggcg tgcgacgcgt gcgcgcccgc cgaacaagcc atcagtgtag agaatgaacg
    11401 catcgccggg tcgaagatcg ataacggttt cgctaaactg cgttggctgc gccacgccta
    11461 acgggggcgc gtgcgacgag accgcctccg cgctcgctcc ctggcgagcg atgagcagcg
    11521 gtggatgccc agcaccgacc acgctggcgc gaccgctgcc ggcttccaac gccacgcaca
    11581 ttgcggtcat aaacgagcgc gcaccgaaga tactgcggaa atcttcgttg accgcgcgca
    11641 tgagtgcgtc cggtgcgccg tgctcgatgc tgccgtgatg aaagagaagt tttgccaacg
    11701 tcgtgaggag ggcggcggaa gcgccatgcc cggtggcatc gacaatccaa aaaagggtgc
    11761 gcgcgtcttt caacgtgagc cagccgtaaa tgtcgccgcc gacttgaatg acggggcgat
    11821 aataatcggc aatgtcccat cccgaaagtg gcggtcgttt ttgcgggatg agtgattgct
    11881 gcgtcaaacg tgccgcggcg agatcgcgct cgagattgcg tcgccattgt tccagctctt
    11941 cgttttgctg ttgcaactgc tcccgcatcg agcggagccg cagctgcgtc tcaattcggg
    12001 cgcgcaacac cgcggtgttg atcggtttgg tgacgaaatc atttgcaccg gcgtcgaggc
    12061 aaagaacttc gctttgttcg tccgtgtgac cggtcaacat gatcgcggga acattcgcga
    12121 cgtccgagtc cgcgtcggcg cgcagctttt tcaagacttc cgcgccgttc aatcccggca
    12181 tttcaaagtc gagaagcaaa agcgatggct gctcacggcg aactaattcg agggcttcgt
    12241 cgccggtctc ggcctcagcg caatcataac cagctgagca gagtgtgcgc acgagcactc
    12301 gacgggttgc tctgtcatca tccacgatca ggatctgccc atcagctttc ttggccatct
    12361 gttttgattc gcaatccgct gctccgtcta ctgtatccga tctcgatcga acaagctggt
    12421 tgcgcaccaa gtcgcgccaa tttaggcatt ggcaaccgct cgcaagacag gttacgtttt
    12481 ttatttagct actcagaact atgaaattct ccctgtcgct tgcgctgctc gctgcctttt
    12541 tcatttccgc ctgttccgac gttgaggaac cgccagcgcg ggtgcggcgc catgttgcgc
    12601 aatatccgcc tgccgggcag gagcagtatc cgccgcaaca gcagccattc aatccgaacg
    12661 ctccgccgcc ggaaaatatg ccgccgcagg agccaacagc cgcgccccca cccacggcga
    12721 tggcgacccg atctgaaaaa ggcgatcacc cctacggcat cccggtccct ggtaaaccac
    12781 acgtcgtgga gagtccattt tcccctggca aatatgtcga tgtcgaagga ttcccgcccg
    12841 gcaccgaggt caaagatccc tacaccggca aaattttcct cgtaccttag cgatcggcgg
    12901 ccgcgatctc agttgcggct catgcgggat gctagatact gggtgaggga tcaaaggcgt
    12961 gtgacgcgtt caggtgttga agcgttttaa tttcttgctt taaccgtttc cgcgctgcaa
    13021 cgcctcaccg atttcttttc atggagttcc gggtcaagcg cgcgccacga atcgacactg
    13081 aaatcaccgt gccgggtgac aaaagcattt cacatcgcgc ggttatcatc gccgctctct
    13141 cgaacggtgt ctgtacgctg cgcggctttt tactgagcga cgattgcatg cgcacgatta
    13201 gcgcgttgcg tgcactcggc atcaggatcg aacaacctga ttcggacacc ctggttgtgt
    13261 atggcaaaaa gcgcgtactc acgccaccga aggaggaaat tgactgtgga aattcggcca
    13321 cgacgatgcg gctgctcgct ggattgcttg ccggccaaac cttcgaaagc cgcttggtcg
    13381 gagacgctgc acttttgcgg cgtccgatgg atcgcatcat tacaccgctg cgccagatgg
    13441 gggcggcgat tgtggccgaa ggcccggagc aaaccccgcc cattcgcatt cacggcggtt
    13501 cgcttcgcgg aatccactac aagtcgccgg tggcgagcgc acaggtcaaa ggcgcggtgc
    13561 tccttgccgg tctgttcgcc cgtggcaaaa ccacctttga ggaacctgtc ccgacgcgaa
    13621 atcacactga gctgatgctg aattatttcc tcgttcgcac ggcgaaggag aacgacacga
    13681 gcgtcagcat ttttggcgac cagatgccgg agtcgcgcga cttcgacatt ccgggcgaca
    13741 tttcttcggc tgcgttctgg ctggtggcag cggcggcgca gcctggcggt caccttctgg
    13801 tgcgtaacgt cggattgaac gaaacgcgaa cggctgcgct cggtgtgcta gtgcgcatgg
    13861 gcgcacaggt gcgcgaggcc atcgaagatg ttgatcaagt cgagccgcgc ggaattgtcg
    13921 aagtaaccgg tgcgccgctg aaggggaccg tcattcaggg caaagaaatt ccgcagctca
    13981 tcgacgaact gcctgtgctc gccattgctg gagcactcgc gagcggcacc acgattatcc
    14041 gacaggctca tgaattgcgc gtcaaagaaa cggatcgcct cgcagcgatc gcgcacaacc
    14101 tgcgcctcat gggagcgcag gtgcttgaga tgaacgacgg cttggaaatc cacggcccga
    14161 ctcctttgcg cggggcgcgc gtgcccagtt tcggagacca ccgcatcgcg atggcgttcg
    14221 ccgttgctgg tctattcgcc gacggcgaca ccgtcgtgca ggacgccgac tgcgtgcgcg
    14281 agtcgtatcc ggaattcgcg agcacacttg aggagttcac gaatcagaaa cggatgcgca
    14341 ccagcacgcc ggtcattggt tccctggccg cgcgtttagg cgaagaataa cgcttggcgc
    14401 atgctccggc agcttgcgca gcagctgccc gtccgagtag atgtcgatca tgcaaaccag
    14461 ttgccattgg gtcgtgacga tcgatggccc agctgcttcg ggcaaaagca gcgtcgcacg
    14521 cgaactcgcc cgccgactac gtttcgttta cgtgaattcc ggtgcgattt accgggcgat
    14581 tacctggtac gttctgcggc aaggggtccc agccagcgac tccgcgcgta ttgcccaact
    14641 ggtcggcgcg gccgcaatcg attgccagct cgaaaacaac gaatcccgcg ttgttattgg
    14701 cgctatcgat ccgacgccgc atttgcgcga accgtctgtc aacgatgccg tctcccaagt
    14761 gagtaccgtc tctgcggtac gccacattgt cacggagaag gtccgtggct acgcgcgcga
    14821 gcacgatttg gtggtcgaag gccgggacat tggctcggtg gtctttcccg atacgccttt
    14881 taagttttac atcgacgcct cgccagatgt gcggctgcgg cggcgcgcag ccgaaggcca
    14941 gcgcgatgag atcgccgcgc gcgactgggt tgattcctcg cgacgctctg ccccgctgac
    15001 catcgcgccc aacgcgcacg tgatcgacag ttcggagctc agcgtaaccg acgtggtgga
    15061 tgaaatcgtt aggcgattac gcgaaacaaa cttgccgatt taagtaatgc ggtaatgcaa
    15121 cagttcagcg gcaataatcg cttaagtgag tagctgatga atttttacta ctggatcggc
    15181 tatcatggca gccggcttct cgcgcaattg ttctttcgct tccgaatcat tcatcgcgaa
    15241 cgcatgatcc aaactggtcc agtgattgtc gcgatgaatc accagagcta tctcgatccg
    15301 ccgctcgccg gaaatgcgtg tgaccgcgcc atctattttc tggccaagaa atcattgctc
    15361 gatgcaccga tccttcgctg gctactgccg aaactgaacg tcattccagt gaatctcgag
    15421 gggaacgacc gccgcgcgct taaaacgttg atccgagttt tacgtgccgg cgaatgcgtg
    15481 ctcgtttttc cggaaggatc acgtaccccg gacggaaatc tgcagccggc actgcccggt
    15541 gtcggccttc tcatcgccaa aacgcgcgca ccggtcgtcc cgatgcgaat cttcggcgcg
    15601 tttgacgcct ggccgatcgg cgggaaactg cggctctggc gcagaattac catcatcgtc
    15661 ggtgagccga tttatttttc cgcggccgat tttcagaacc ccggcagaac cacatgcacc
    15721 gcggttagcc agcgtgtcat ggatgcgatt gggggtatca aaatgcccga agcaaagatc
    15781 gacattcgca ggaccgacaa tgacgttaag ccagcaacgt caacctcgat aattcgccgg
    15841 tgaatccgcc gccaacctcc cgcgtgtgca aagatgcgat tgtcgtgatg attataattg
    15901 tcgcggtcat ggcatttgtc gccatctatg ccaacgtgca gcgctggcgt cgggacaaac
    15961 tcgagcacgt gattatcacg ccagttgcat ctcccacggc ttcaccagcc gcgccgtgag
    16021 ggtttcgaat aacaccgcac caccagagtc taaggctggc atgaacagat ttctgatttt
    16081 ctctgcggct ggtctcctcg cgtcgtttat tggttgccag aaacagcaga gtgaggcgga
    16141 acgaaatgcc gaggtcgagc ggcaggtgaa cgagcggctg gcagctgaac atcaggcgca
    16201 ggaacagcaa cgcctggtcc agcgccagga tgaactcgac gcgcgcttgc gcgagttgaa
    16261 tgaacaacaa cgcgcggccg cgagcacgcc cgagccgcag cggaccgaga ccgagcgcag
    16321 cacgcgcgag actttcgaag cgcaacccgc cgcttcatac tcgatgttct acaccaagct
    16381 cgaaccattc ggcgtctggc gcgagacccc gacctacggc tacgtctggc aaccgcgcga
    16441 agcgatgcaa tcgcgtagtt ggcggccata cacaaatggc cattggattt acaccgacgc
    16501 gggctggacg tggatttccg aggagccttt cggctgggcc gcataccatt atggccgctg
    16561 gatgcgattg cgcgggatcg gctgggtttg ggttcccggt agcgaatggg cgccggcctg
    16621 ggtctcatgg cgcacgagca acgattacgt cggttgggcg ccgctgccgc cggaagcgcg
    16681 gttcgaacgc gatgccggga tcaagaattg ggccgacggt tattacgata tcgggccgga
    16741 gcaatattgc ttcgtcccga cgcgcgagat tggaacccaa cgaatcgagc gcgttgttgt
    16801 gccgcccgag caaaacgtca cgatcgtgaa ccaaaccacg aacgtgacca acatcactta
    16861 caacaacagc atgattgtga accaggggcc gaattacgac gagctgcgcg cgcgcagcca
    16921 acagcctatc caacatctgc gattggagcg ccggacaacg gtcgaaatgg agaacccccg
    16981 tcccattgtg aaaggggaag tagttgaaat gcctgcaccg gtaatcgccg caacacagcc
    17041 agtcgagcgg ccggcgacgg taagagaaac agtcgcagcg gttgtcgaac ggggctggga
    17101 aggaattgcc gatcagcaag cggtgcagcg agcgcgtgca aaaataaaat ccgaagcgac
    17161 gccgccgccg aacgcgccgc ccaaaacttt catcaaaccc gcgccggcca ccggacagac
    17221 ccgtgcgact gtgccgtctc cgcgcccaat agcgacggcg attgcaagat cgacaatcac
    17281 gcctccggca acatcagtgg tgcaaacgcc aacgcctacg caggccgttc gcccaacaca
    17341 gactccgata ccgagtcgtg cggcagctcc atcggcgccg cctactgaag gtgccgtcca
    17401 tacagcaact ccgatcgcaa cgccgacacc agcaatgctg ccaacccgac tcgccaccgc
    17461 aacaccgcgt ccgacggcga cagctgttgc aacgccgact gccatcgcgc gcccgacctt
    17521 ttcgccgcgg ccatctgcaa cggcggcttt ctcgccgaca ccggcggcgt ctccgacatt
    17581 tggacgcgct ggaaccaagg agcagcgcga ggcgcaaaag gaattgcgga agcaggaacg
    17641 cgctgcacgc aaacaggagc gtttggaacg tgcgacgcca acccctgccg ccactgcaac
    17701 tgcgtcggtt cctgcgtcag catcacccgg cgcggccgag tcgccgggga aacacaaaaa
    17761 gcgtcctggc gaagctgcaa cctcaccaac cgctacgccg acggcttcgc cttctcgtta
    17821 ggtgtatttt ccgccgagct gcggctctgt tgggcggcga gttcgcgcgc gtaagccagc
    17881 gtgtcgattg catctgccgt tttgtcgcgg cggatcgctt taatccgcgg gaatcgcaac
    17941 gccagtccgc ttgtgtggcg cgtgctcggc tgaatcgagt tgaacgcgac ctccagcacg
    18001 atgtccggct tcacttcgcg ataacggcca tgatcggcaa tcgtgttttg cttgaagtgc
    18061 tcggtcagct cagcaatctc ggcgtcggta agtccgctgt aggctttgcc gattgggagc
    18121 agctcgccgg tagtctcctc gcgcacggca aacgtgtaat cgctcaggac atgattgcgt
    18181 ttgccgtggc cgagctcggc cgcaacgacc accacatcga gcgtcgccag ttccttcttt
    18241 aacttgtacc agaacatgcc gcttcgccct ggcatgtaaa aactttccgg gtcttttacc
    18301 atcagccctt cgttcgcgcg gcggcgcgct tgctgaaaaa tctcctccag ctcggcggcg
    18361 gagtgcgcgg ggaaggtctc agccgcttcg aatcgcggcg gcaaacgcac gcttcgcaga
    18421 agttcacgac gttcccgcaa cggtgtcttt aaaagcgagc gtccgcttag ccaaagcaaa
    18481 tcgaaagcga tgaacgcgac cggcacatcg gccgaagccg cgtcgaagag atcggcgccg
    18541 tcacttctgc ggccgagtcg cttttgcaga tcgaagaacg taagcttgcg tccgtgctcg
    18601 aacgcgacga tctcgccgtc aaggataaga tcgacatcga actgccgcgc cgtattcgcg
    18661 agttccggaa actgttcggt gatctgcttt aaatcgcggg agaaaatctc tgcgcgttgc
    18721 gccgagtgat gcagctgcgc gcgaattccg tcgaatttgt cttccgcata cacaaccttc
    18781 gcatgttcaa agcgcgccca gatcgcttcg gcggtaggtt cgggagtcgc gagcatgcat
    18841 ttgatcggcc ggaagatcga caactccgcg cgatccaact cttttttcga tgcgagtaat
    18901 gcggtcgctc cgatgtcgcc aagcagcatg tttgcttctt tcacgtcctc cagtggaaca
    18961 tcaaacgctt tcgccactgc ctcctcgacc aagccttcgc gcaaaccgat ccgcaggtcg
    19021 ccagtcaaaa tcttaaccac gtactcgcct tctttggccg acaagatcga cattcgcttt
    19081 tcgagcagtt ccgctttcgc aatcggtcca cgcgcgcggt gcaaattttc aaacaacttg
    19141 cgggattcgg aaatcgaaaa cggctccggt tgcgtacgac tcgcgagaat ctcgaacgcc
    19201 gctttgccgg catcgccatg tttggctgcg atgcggtgga actccgagtc gctaattttg
    19261 gttgcggctt gcagcgctcg aaaaatcacc gaccaaccaa cttgcagcgt gcgcggatca
    19321 ctctgggcaa aggcgcggcc ggtgaaatat gttgtcgcaa tcgcgagctg cgccgggctg
    19381 agactgcgca ggtaatcgga aagcacgcgc accttctcga gttttggcgg cgtggcggca
    19441 atcgcttcgc caattcgggc gaactcgccg aattctgact ctatagtggc cgttgtctca
    19501 acggcatggg cattgccgcc ggggacggcg ggtgccacag ggccggcatc caaattcatc
    19561 tcgatttgat tttcctcgcc taacgcccat gcgtccatgc cacgcgcgcg caagtcgcgc
    19621 gcgaactcgg ccgcaaatcc atgcagggtg agaacgcgtt tcggttgtac aaggtggaca
    19681 taccggatca ggtcgtcgta atctgcatga tcggaaagcg ggaaggcggc atcgacctgg
    19741 tagcgataga ccgcatttgc atcgacggcc cacccgctga tcatggcggt gcgtttgcgc
    19801 ggaatgttgt caatcatgcg cgagtggttc gcgcttggcg gacagatcag gactttgcct
    19861 gcgaggtcgt ttggattgta acggacgtac ttacagaacg agcggccgag ttgctcgtag
    19921 acgtgcgtga tttgaaagac cgagccatgc agcatcggca cgagcccggc gccttcgagt
    19981 gcgcagagaa tctcctgcgc tttgcctaac gaatagccga acagcaccgg gaccacgccg
    20041 tcgtccagtg cctcgcggca gaacgcaaca atctgatcaa cgacttgttc ggtcggcgga
    20101 aagcggtagc gcggctgtcc gaacgtcgtt tccatgatca gcgtagcggc gtgcttccac
    20161 tccgccagtt cggccgattt gccgcggcgc aacttgaaat cgccggtata aaggagcgac
    20221 tcgtttgcag tctcgagata gagctgcgcc gaaccaaaaa cgtgcccggc cggcaacagc
    20281 ataagatcga caccatggaa gcgccgcttc acgccgaaag gcaatacgtg ttcctgccgc
    20341 tctccgggca ttcgcgcgcg cagcagccgg gccgtgccgg gcgaggcgat aatctcctga
    20401 tgcggcgcga tatgatcgct atgcgcgtgc gacacgaacg cgagcggttt cgcgtcccac
    20461 gggtcgagcc acaaatgcgc ttgcggcagg taaacaccac gctcgtaacg gacatccatg
    20521 acagctccaa ttttaagctg aaatgacagc gaaagctcgc gcccgcggcc ggcgcattga
    20581 caggctcggc ggtggcgggt ttgattggag ccgaatgaag acggtttcgc tggcgctgct
    20641 tctagggttc tcactgccaa ctgctcttcg cgccgagtcg caaccggaac cgaagctgct
    20701 tgcgaaggtc acgccgttgc ccgtggcgct cagcgacgat ttcgacttcc gcaaagtgaa
    20761 actttatttc ttgtccgaaa aaccgacgca gctgaagacg aggcgttcca tgtcgcttaa
    20821 cccgaacgcc tatgcaaccg acacagggca acaggccgtg gttaaccagg acagctcgct
    20881 tagtttcgag cggcaatacc ggttattcgg ggcggtcacg gccctggacc agcgccagcg
    20941 gctcggagac tatttggatt tcttctggcg cgcgaaacgg ccggccgacg tgacggtgcg
    21001 gctggaatat cgccaggaaa agttgcacga tctcgtgcag gcgcaggagg tctcctacac
    21061 gaacgtgcac ggcacgcaca aaaccgaatt tagagtcatc ggcgatgact acctggatga
    21121 cgggcgggtg acggcctggc gctgcctgct cattgagaac ggccggatcg tagcggtgcg
    21181 gcgttcctac ctgtgggatt aagcgggagg cgcactcgcg ccgacaggtt tgacagcgca
    21241 attgtcgacc ctgtatctta ttatttgttt agcagtggaa tcgtcgattt tagttggagt
    21301 gagcggcccg gccgtttgga tcaaggtcga aggtaaaggc agttttctga acagcgggaa
    21361 cttgaaggag ttcgccaaac agatggtcga ccgcggctat cgccagttca tagtggatct
    21421 cgcagattgc gcgatgatgg attcgacctt catgggcacg atggcgggga tcgccttgcg
    21481 cttaaaggag ctcggtcgtg ggcatctgca catcgtgcac tgcggcaatc gcagccacga
    21541 actgctggca ggtctcggcc tcgaccacgt attcgacatt cacaccaacg gttcagtcgc
    21601 gccgaaatgc gagacgctct cgcgacaggc gagcggcgag agcgttatcg agcaaaagca
    21661 ggagcgcgcg caaaccatgc tccaagcgca cgaagcgctt tgtcaggttg ctcccgaaaa
    21721 cgttttccgg ttcaaggacg ttctcgatta cctgaaacaa gaccttcatc aaaagacagc
    21781 gtcgaagtaa gctgacgctg tatcatgtcg ccgagtcttc ttttcgggct gctcctcctc
    21841 tgcattgccg gttggatcta cactacgctg ctactgtccc gccgcctgcg cgtgctcgaa
    21901 cgctcgaaag aggagattca ggtcgaggag acgttggtat tcgatttttt gcacgggctg
    21961 ggcgaagcct tcagcgaaac gattcgcccc aaccatttgc accgcctcat cgtcgagggt
    22021 gcgacacgcg tgctcgatgc gcatggtggc gcgctttaca tggtcgaccg cacgggcacg
    22081 aaattgttgc ctgcgtttgt ttccaagggt tgtccgccgc tagttgaaat cccggaagat
    22141 gtccttgcgc aggctgccgc cagcccagcg gcactcgaga gctatgtgcg gcttcgcgcg
    22201 gtcggggccg gggaagggac catcggccgc gtctggcaaa tgggtcaggt aatttacctt
    22261 aacgaactgt cgcaagcccc tgagctggca aaactgcgtg acagctcctt cggcgcagcg
    22321 tctgtcatga tgggtgcgct gctttacgcc aaacaaaaca tgggtgtgct ggcggtcgcc
    22381 aatggcccga tgggctcccc gttctcacaa agcgacttcg tcgttttcaa atccattgcc
    22441 gagcagtcgg ccttcgctct ctacaacgcc atcatctact cggaagcgaa cgagaaaaag
    22501 cgtctcgatc acgacctgga gatcgcgcgc gatattcagc gcattctttt acccgccgaa
    22561 gcgccagcgg tgaacggatt cgaactcaac ggcatcaatg tgccagcgcg gcaagtgagc
    22621 ggagattact tcgactacat caaggtggat gacgagcggc tcggggtcgc gatcgccgat
    22681 gtttccggca agggtgtccc cgcctcgctc atcatggcta tttgccgcag tgtcctacgc
    22741 agccaggcag cgcagaatcc gtcgcccgcc gatgttttgc aaaaggtgaa ccggcagctt
    22801 tatcccgata tcaaagagga catgtttatc agcatggctt atctggtgct cgatcacgcg
    22861 cacaacggca ccgtcaccct cgcccgcgcg ggccatgacg cgccgttgct ctacaaacgg
    22921 agcgcgcaaa gcgtcacgcg gttgaagccg cctggtatgg tcctcggcat tgacagtggc
    22981 agcgtgttcg atagactcac aaacgacgtc gccgttccgc tcgaacgcga tgattgccta
    23041 gtcctctaca ccgatggagt gactgaagcc attgacggcg aaggcaacga attcgggctc
    23101 gaacggatga tggagtctgt gcgcgcgagc gccacgaatg gcgccgccgc cattgtgacc
    23161 cgactcatcg acgacctgcg cagcttcgtc ggcgcgcgtc cgcaaaatga tgacgtcacc
    23221 ctgatcgcca tccgaaagac atgaaaaaaa ttccagctac cgactccggc gcggacaaga
    23281 aacagaacgg cgcacctgcc tcggctgccg caaacgcaac atcgacactc cacgaggaga
    23341 agccgaccga atcttcggcg actgcgcaat cggaaacaga ggacccgatg gccggtttgc
    23401 aagccgacct tgaccgcttc cgcgacttgg ccctccgcag tcaggccgat ttcgagaatt
    23461 acaaaaagcg ttgcgctcgc gagaaggaag aagcagtcaa atatgccaac agctcgctgc
    23521 ttgagcgact ggtcgcaatt gtcgacaact tcgagctggg tctgaacgcc gcgcgcgaac
    23581 aaagcatgga ctcaccgatt tattccggca tgactctcgt cctgaaacaa ctcaacgatt
    23641 ttcttgctga gaatggcctt cagccgatcg aagctgtggg cgagaaattc gatccgaatc
    23701 ggcacgaagc catcgcacat gagccaaaca gcgacgctcc cgaaggaacg gttattcgcc
    23761 aggtccgccg tggctatcgt ttcagggacc gcctcctgcg ccccgcaacc gttgtcgttt
    23821 ccgccggccc agccaagcac agaaccggtg ccgagtaatc cgggcgcggc gtttgctcag
    23881 catttgtcac tcgtccttcc tcacttctca ctatcttgat ggctacgaag aagcgcgatt
    23941 actacgaagt cctcggcgtc ggacgcaact cctccgagga cgagatcaaa cgcgcgtatc
    24001 gcaagctcgc ggtcaagttc catcccgata aaaatcccga cgacccgcac gccgaggaaa
    24061 aattcaagga actgggcgaa gcctacgacg tgttgatgga cgccgacaaa cgcgccgcct
    24121 acgaccgttt cggtcatgct gcgttcgcac aaggtggcgt cggcgccggc tttcatgatc
    24181 cgttcgacat cttccgcgaa gtcttcgccg gcgcgggtgg cggcattttc gagacgtttt
    24241 tcggcggggg gacaatgcgt gcggaagatc gacaacgtgg ttccgatctg cgttacgaca
    24301 tgcagatcac gttggaagaa gcggcggcgg gcgtggaaaa ggagattgaa attcgaaagc
    24361 tcgacacctg cgataaatgt cacggcagcg gcgccgagcc gggctcgcgg accattacct
    24421 gcccaacctg cggtgggcgt ggacaggtca tcagctcgcg cggcttcttc caagtttccc
    24481 aaacctgtcc gcgttgccgc ggcatcggac acatcatcga caaaccgtgc cgccactgtc
    24541 atggcgatgg gcgtgtcgaa aagctcggcc gcatcaaatt aaaaatcccg accggaattg
    24601 cggatggctc gcggctgcgt tcttcacgca atggtgaagc cggcacgcgt ggcggtccgc
    24661 acggcgatct ctacgtcgtt gttcacgtaa aggaacacaa aatctttcat cgcgaagatg
    24721 acgatcttta ttgcgagctt ccgattccat tcacagttgc agctctgggc ggggaagtcg
    24781 aagtcccgac gctcgaaggc aaggcgcatc tcaaagttcc ctccggcacg cagagcggtc
    24841 agatcttccg tttgcgcggc aagggcattg tcaacgtaaa tggccgcgag cgcggcgact
    24901 tgctcgcccg gctcatggtc gaagtgccta cgcgcttaaa taacgagcaa cgccagaaac
    24961 tcgaagaatt cggcgcgctc tgcggggacg aaaacacgcc catccgcaaa agttttttcg
    25021 agcgcgccaa ggaattcttc gggtaatctg tagtggcggt cgttcctcgg ccgcagctta
    25081 gtggcaacgg cagtatgcct gcggtacaat gcatcgattc tacatctcac cgcagcagtg
    25141 gaatcccgat ctcctggttt tgaccggcgc gcaagcgcat catgcgcgcg acgtgctgcg
    25201 cttggggcgc gacgacaagg ttgcggtttt taacgggcgc ggccgcgaga tcacggcgaa
    25261 gattgtcgat gttagcggac gcgaagtgtc gctagcgaaa ctcactcaga gcgagactcc
    25321 gccgttgcga tcgaggattt tgttgggcca ggcaattcca aagggcaaaa acatggacct
    25381 gatcgtgcag aaagcggtcg agcttggagt ggccgagatt tttccgctca tttccgctcg
    25441 cacaattgtc gatcttgcgc aggacgaggc gcggaaaaaa caggcaaaat ggcaaaccgt
    25501 cgcgatcgag gcggccaagc aaagcgggca aaattggctg ccgcaaattc accttcccca
    25561 aaccatgaag cagtttttcg ccgaactccc gccatccgat cttcagctga tcggctcttt
    25621 gcaacccggc gcgctgcacc tgaagaaaat cctcgctgag tttgccgcgg aacatcgatg
    25681 tcgtccgcac agcgtgctca tgcttatcgg gccggagggc gatttcacgc cggccgagct
    25741 cgcgctcgcg cgcagtcacg cgtgtcggcc gatcaccctc gggccgatca ttctccgcgt
    25801 cgaaactgcg gcgatttact gcctgagcgt tctctcctac gaattgtttg cgcctcagta
    25861 aaggtagggc gcaggcagct gaaacgcgtt cttgaccagc tcgatccgcg gctctgcgtc
    25921 atcgcggacg aggatttcaa cgacgtcgaa ccggaataga acgtcagcat tatcaagcaa
    25981 gcgcagccac gccaacccgc cacgcgagat ccgctttcgc ttttgccgat cgactgcgct
    26041 gagcggccgg ccgaactctt cgtgtgtgcg agtttttact tcgatgaaaa cgagcgtgtc
    26101 cttgtcgcgc gcgacgatgt cgatctcacc accgctgcgt cctcggaaat tgcggtagag
    26161 aattttatag ccgttcttcc ggaggaagcg ccgcgccaga ttctccccca atgcgccgcg
    26221 ccgaatgtgt tccggctggg acgaattcct ccaaggcaag aagcggctgc gccaccggtt
    26281 caaaagaccg gcgatggatc ggacaaggcc cgaattcatg cagtttcgtt aagtgcagct
    26341 cggtcgtgta gcctttatgc tgactgaaac agtattccgg aaattgcgcg cagaagtcac
    26401 gcatcagtcg atcgcgcgtg accttcgcga tgacgctcgc ggcggcgatt gtcttgctga
    26461 cgcaatcacc gtcgatgatg gcggtttgcg gaaaatcaaa cgggaacacg gggagcccgt
    26521 cgatcagcac atggtcgggt tggagggcaa gcgctgcgag cgcggcgcgc atcgctttgt
    26581 gcgtcgcgcg caaaatgttg atcgcatcga tctcgatgtg atcgaccacg ccggtcgccc
    26641 agatcacctc cgcgttggtt gtaacatcgg cgtaaacttt ctcgcgcact tcgggcgcga
    26701 gttgtttcga gtcgttcagt ttccggtgcc gaaatttctc cggaagaatg acggccgccg
    26761 ctaccaccgg tccagcgagc gcgccgcggc cggcttcatc aatcccggcg atatgcgcca
    26821 cgccggcggc gcgcaagttc ttttcataac gaaacccgca gcgccgatac atctgctcgg
    26881 cagattatcg aatttccgaa tcggggaaat tggaaaatgt gatgtgtgaa cggtgatcag
    26941 tccttccacg ctgttctgtt caccgatcac tgttcacgga tcgctattct cttactgcgg
    27001 tcgcttcctt gcccttgcgc gtgcgcagat aattgagctt ggcgcgccgg gcgcgacctt
    27061 gcttttccac ttcgattttc ccgatgcgcg gcgagtgcag cggaaatacg cgctcgacac
    27121 cctcaccata cgagatgcgg cgtaccgtga acgtttgatt taagccgcgg cctttccggc
    27181 caatcacgat cccgctgaag atttggatcc gctccttgtc gccttcgacc acgcgggtgt
    27241 gcacgtgcac gctgtcgccg acggagaatt gtgtgccgtc ggcttttcgc tgctctttct
    27301 cgatggcatc aatcaggcgg ttcatagcaa cttggcggcc gccgcgtggc gggcggacat
    27361 cttcgattga gccggggcga aatgcaaacg caagatgccg atcttgcgtc gtgctcgtgg
    27421 tcgtaagcgc acaaaatcga gcatgagcac gattatgagc cgagcgcgag ttgggctgcg
    27481 ccagaaattg tctatgaaag tttgcgtagt ctgtggctaa attctcgcgc tcttacgcgt
    27541 ccgtagctca attggataga gcgttggcct ccggagccaa aggttgtggg ttcgaccccc
    27601 gccgggcgca gtttttccga tgtgatcagt gatctgtgat cggtgatcgg tcgaagcagg
    27661 taggtcggac cggatcactg tttacggatc accgatcact gaaattccgc gcagcggaat
    27721 ttcctcagcc cagcgtgtcc agccgcgcga gcagatcctt tttgctcgtc acgccggtaa
    27781 cctgatcgcg taactgaccg ttcttgaaaa agagcagcgc cggaatggcg cgaatgttgt
    27841 atttgaacga gaggctctgg ttttcgtcga cgtttacttt tgccaccttg accgcgccgg
    27901 ctttttcccg cgcgatttca tccagcactg gtgcgatcat cttacacggg ccgcaccatt
    27961 ccgcccaaaa atcgacaatc accggcttgt cactctgggt aacttcgcga tcgaacgtgg
    28021 attcatcgac agtgatggca gcgctaggtt ccatagatca cgaaaagtaa ttccaaatct
    28081 ggaggatcaa aagcacagcc gagacggcga ggatggtcca gcaaagtgcc gcggggatcg
    28141 tttcgacgcg tgcttccgtg tcgccactga ccatgagcgg cgtgggcgca gcctctgccg
    28201 gttccggcat ggtaaagagc ggctgcgttt ttttcatctc ccccgccggc gccggactcg
    28261 gcggctcggt cggtaagggg atccgcgccg tttccttcct cggtggctca tgcggcgagg
    28321 caggcggcgc tacattttcg gttggcggcg gattgggcgg cggaggcata ttcacaggcg
    28381 ggcgcgccgg caagttgatg cgctcacttt gcgcaggggc ttcgctgtca ccggttttcg
    28441 tcggcgcgac gattcgcacg gtttctcttt tgggctggtt aggctcggcc attcggttgg
    28501 tattaaatgc tgcgcgaaaa ctctttcgtt gtcactcgtt aacttgtttc cacctaggcg
    28561 agcaggtcgt agccgcggct accgccaatg gtgcgccaga caaattcgcc cggtaccgcc
    28621 gcttcggaca gaatcacgcg cgcgtctacg tcgggcgcgt cagcttcagt gcgcgcaata
    28681 tgcggttcat cgacaagcaa cttgagttta tgtccaattc tttcacgcgc gagctcgtac
    28741 gcgatttttt gctggagcga catcgccacg cgatggcgcg cattttttat tctggcggaa
    28801 atttggtccg gtagcgtcgc ggcgcgggat ccctc
//
LOCUS       DASTDG010000159         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1207607, whole genome shotgun sequence.
ACCESSION   DASTDG010000159 DASTDG010000000
VERSION     DASTDG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207607"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /gene="thrC"
                     /locus_tag="VFO30_05345"
     CDS             complement(<1..583)
                     /gene="thrC"
                     /locus_tag="VFO30_05345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9418746.1"
                     /translation="MIDRYREFLPVSDLTPVISLGEGDTPLIFSPRLTQRVGRGCEVF
                     IKYEGLNPTGSFKDRGMTIAVSKAIEGCACALICASTGNTSASAAAYAARAGVSCAVI
                     LPAGKIARGKLLQAFVYGAKILAIDGNFDDALRIVRELGASNECVIVNSINPDRIAGQ
                     KTAAFEIVDVLGDAPDLHLLPVGNAGNITAYWSG"
     gene            687..>2875
                     /locus_tag="VFO30_05350"
     CDS             687..>2875
                     /locus_tag="VFO30_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9418747.1"
                     /translation="MPGLSKEDKLRLLELILQSRHADLREQNLNRQGKGHFHVSGMGH
                     EALAAISIQLQPDDYIVPYYRDRGLVLGRGMTTRELALEYFAKRKTGSGGRMMPSHYS
                     DAARHIWSVPTPTGSQLLPACGIAWGIKLDGKNSLVVTTVGDAATRQGDFFEAICFAK
                     EKKLPALFVVEDNAYGISTPTRKINPLALKALEAKDWQRIDGGNVEQVYEAAAAALDK
                     IRAGNGPIFLWVMMERLSSHTSSDDQKLYRTPEELAACEKADPLACWKEKLIQQGLLT
                     ADEFAKIDNEVKERIRHEYSEAERDEDPSPNELEANVTGQLPKLNDEILPPGKYRIGD
                     TINKTLRLGLERDPRRVIFGEDVEDPKGGVFRLTQKLSTDFPNQVFNSPLAESTILGI
                     ACGLAAYGRRPVFELQFIDFVYLGMNQLVTNISNLRWRSFGNWKCPAVIYAPYGAYLP
                     GGSLWHSQANESVFAHYPGLIVAIPSTPEDAAGLLWTAMHSEDPVLFLIPKHMFWAER
                     ETAKPVRAVPFGNVRTVCPGEDVTVVAWGNTVEKCVEALAKIDNATSVELLDLRSIVP
                     WDKAGIFESVRKTRRLVVVQEDTENCSVGQMIISHAVADVDLWNTMVSPPVLVSKGNV
                     LIGYNPIYEYAALPDVSRIIAAINRSVSTVETARPRAAVSRHATRPDRPEAEPAPEPR
                     STTAATDHIQPITVPIMGEGIRNAKVVSLLKNPGDPIALDDELCEVET"
BASE COUNT          648 a          857 c          813 g          557 t
ORIGIN      
        1 aaccgctcca ataggccgta atgttcccgg cattcccgac cggcagcaga tgcaggtctg
       61 gcgcatcgcc aagaacatcg acaatctcaa aggccgcagt cttctgcccc gcgatccggt
      121 caggattgat cgagttcaca atgacgcatt cgttggatgc gccgagttcg cgcacgattc
      181 gcagcgcgtc atcgaagttg ccatcgatcg cgaggatttt cgcgccgtaa acaaacgcct
      241 gcagtaattt gccccgcgcg attttgcccg ccggcaaaat taccgcgcac gaaacgccgg
      301 cgcgcgccgc ataagccgcg gccgacgcgg acgtgtttcc cgtggatgca caaataagcg
      361 cgcaagcaca gccctcgatc gccttggaaa ctgcaatcgt cattccgcgg tctttgaatg
      421 agccagtcgg attcaagcct tcgtatttga tgaagacctc gcagccgcgg ccaacccgct
      481 gggtcaaccg tggagaaaaa atcagtggcg tgtcgccttc gcctaacgag atgactggcg
      541 tcaaatcgga aacgggcaag aactctcgat accgatcaat cactcccctg ccctgctctg
      601 actttgtcgg tacggtcatc ggcactccat tcgaacaaat tcgcacaatt gtcgaccgac
      661 aactgcggtt gtaagatcga catcgtatgc cgggtttaag caaagaggac aaactgcgcc
      721 tgctcgaatt gatcctccag agccggcacg ctgatttgcg cgagcaaaac ctcaatcgcc
      781 aagggaaagg ccatttccac gtctctggga tggggcacga agcgctcgcc gcgatttcga
      841 ttcagctgca acctgacgat tacatcgttc cgtactatcg ggatcgcggc ctggtgctcg
      901 ggcgcggaat gaccacgcgc gagctggcgc tggaatactt cgcgaaacgt aaaaccggca
      961 gcggcggtcg catgatgccg tcgcattaca gcgacgctgc tcggcacatc tggagcgtgc
     1021 cgacaccgac cggctcacaa ctcctgcctg catgcggcat cgcctggggc atcaagctcg
     1081 acggcaagaa cagccttgtc gttaccacgg tcggcgacgc cgcaacccgc cagggcgatt
     1141 tcttcgaagc gatttgtttc gcgaaggaaa agaaactgcc ggctttgttc gtggtcgaag
     1201 ataacgccta cggcatcagc accccgacgc gaaagatcaa tcccctcgcg ctcaaagcac
     1261 tcgaggcaaa ggattggcag cgaatcgacg gcggcaacgt tgagcaagtt tacgaagcgg
     1321 cggcggcggc gctcgacaaa atccgcgccg gtaacggacc gatattttta tgggtgatga
     1381 tggagcgcct ctccagtcac accagttccg acgatcagaa actctatcgt acgccggaag
     1441 agctcgccgc ctgcgagaag gcggatcccc tcgcctgctg gaaagaaaaa ctgattcagc
     1501 agggtcttct cactgctgac gaattcgcca agatcgacaa tgaagtaaag gaacggattc
     1561 gccatgaata cagcgaagcg gaacgcgacg aggatccgtc gcctaacgaa ttggaagcga
     1621 acgttactgg ccagctgccg aagctcaacg atgaaattct cccgccgggc aaatatcgaa
     1681 tcggcgacac aatcaacaag actctgcgac tcgggctcga gcgggacccg cggcgcgtca
     1741 tcttcggcga agacgttgaa gatccgaagg gcggcgtgtt tcgcctgacg caaaagctgt
     1801 cgaccgattt cccgaatcag gttttcaact cgcccttggc tgaatcgact attctcggga
     1861 tcgcgtgtgg cctcgccgcc tacggccggc ggccggtatt cgagctgcag ttcattgatt
     1921 tcgtttatct gggcatgaac cagctcgtga ccaacatctc aaatttgcgc tggcgctcat
     1981 ttggaaactg gaaatgtccg gcggtgatct acgcgccata cggcgcgtat ctgcccggcg
     2041 gcagtttatg gcatagccag gccaatgaat ccgttttcgc gcattacccc ggcctcatag
     2101 tggcgattcc gagcacgccg gaagacgctg ccggtttgtt gtggacagcg atgcacagtg
     2161 aagaccccgt tctgtttctc attcccaaac acatgttttg ggcggaacgc gaaaccgcga
     2221 aaccggtgcg cgctgttcca ttcggcaacg tgcgcacggt ttgtccgggc gaggacgtaa
     2281 cggtggtggc ctggggcaac acggtcgaga aatgcgtcga agcgctcgcg aagatcgaca
     2341 atgcaacgag cgtcgagctg ctcgatctgc gctcgatcgt gccgtgggac aaagccggca
     2401 ttttcgaatc agtgcgcaaa acacgccgcc tcgtcgtcgt tcaggaggat accgaaaatt
     2461 gcagtgtcgg tcagatgatc atctcgcacg ctgtggcaga tgtcgatctt tggaacacga
     2521 tggtcagccc gcccgttctc gtcagcaaag gcaacgtatt gatcggctat aacccaattt
     2581 acgaatacgc ggcgttgcca gacgtctctc gcattattgc cgcgattaat cgctcagtct
     2641 cgactgtgga gacggcgcgc cctcgcgccg ctgtcagcag acatgccacg cgtccagacc
     2701 ggcccgaggc cgagccggct ccagaaccgc ggtcgacgac cgcggctaca gatcacatcc
     2761 agccgatcac ggttccgatc atgggcgagg gcattcgcaa cgcaaaagtt gtctcgctcc
     2821 tgaagaatcc gggcgacccg atcgcgctcg atgacgagct ttgcgaagtc gagac
//
LOCUS       DASTDG010000160         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_575207, whole genome shotgun sequence.
ACCESSION   DASTDG010000160 DASTDG010000000
VERSION     DASTDG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575207"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..784)
                     /locus_tag="VFO30_05355"
     CDS             complement(8..784)
                     /locus_tag="VFO30_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(362..364),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9418748.1"
                     /translation="MNGRWNWRLWVGCVLVLLGVFSYPALAQFPITRDLPWLNLLLFC
                     AGGLLLATGLVRAYSESQIYRGKVFGAVLAAISVFISLFVYEIFYVLRQVPRSAHAPR
                     VGEKAPDFALPDQNGKRVSLTDLVSPNGAVLIFYRGHWUPLCNSELRSFQQRLPDFTS
                     RGLHVATVSVDSVDTNRAHSRKMGFTYPLLSDADAAVIRRYDLLHAGAGPKKSDVARP
                     AEFFVDASGTVRWVNLTENIAVRARPEQALAAIDAALARP"
     gene            complement(781..2757)
                     /locus_tag="VFO30_05360"
     CDS             complement(781..2757)
                     /locus_tag="VFO30_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HET9418749.1"
                     /translation="MRKELTVRGLIIGVIITLVFTAANVYFGLKAGLTFSTSIPAAVI
                     SMAILRGFRDATIQENNIVQTVASAAGTLSSIIFVLPGLIMIGFWTEFPFWTSFWICA
                     LGGILGVMYSIPLRRALVTTSDLPYPEGVACAEVLKVGSSGHSDHAHDIEHGRAGLLA
                     VLWGSIVSAVFAVIVATQMFAADVAQTFRIGKRGAVTGYDFFLSFALLGIGHLVGLSV
                     GIAMLIGALIGWGWGVPHYSSLAGDMTTAVARLATRTWSTKVRFVGAGAIGVSAIWTL
                     LKLIKPVVRGLTSAMAASRARKAGKADTLPITERDIPIGIVGLATLACMLPIGWLLGD
                     FGNASGLGAHLPTLIIGGVVYIVLMSFFVSAVCGYMAGLIGSSNSPLSGIGILVVIGA
                     ALLLVLGVKPYVGPDASKALMAFALFTTAVVFNVAAIANNNLQDLKTGQLVDATPWKQ
                     QWALVIGVIAGAFVIPPVLDLVNHAYGFVGAPGAEARPSPLPAPQAGLISSLAKGVIA
                     ADIDWSLIRIGGAIGVGIILLDEILARTTKQMRIPPLAVGLGIYLPTQSTLMIVVGAI
                     VGWFYDRRADRTPRPDSAKQLGVLLASGLIVGEGIIGVVISAIVVFSGKDAPLALVGT
                     GFATTAIIIGGVAFAAIAIVLYRWILRMAAARSI"
     gene            2968..>3218
                     /locus_tag="VFO30_05365"
     CDS             2968..>3218
                     /locus_tag="VFO30_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9418750.1"
                     /translation="MAKTRKTRSVTKHDQTQARESTQEKLRVSEEQFRVLVESVEEYA
                     IYLLDPQGNVASWNLGAEKLKGYTADEIIGKNFACFYTD"
BASE COUNT          790 a          961 c          924 g          543 t
ORIGIN      
        1 ggcgaagtta tgggcgcgcc aaagcggcgt caatcgcggc cagcgcctgc tccggccgcg
       61 cacgtacggc aatgttctcg gtgagattta cccaacgcac tgtgccgctt gcgtcgacaa
      121 aaaactcagc gggacgcgcg acatcgctct tcttcggtcc cgcgccggcg tgcagcaggt
      181 catagcgccg aatcaccgcg gcgtctgcgt cggaaagaag cgggtatgta aaacccattt
      241 tccggctgtg tgcgcgattg gtatcgaccg aatcgacgct cacagtcgcg acgtgaagac
      301 cgcgcgaggt gaagtcgggc agccgctgtt ggaaactccg taactcggag ttgcaaagtg
      361 gtcaccaatg gccgcggtag aaaatcagca cggcgccatt cggtgaaacc agatcagtca
      421 gcgaaacgcg cttgccgttc tggtcgggta acgcaaagtc cggcgccttt tctcctacgc
      481 ggggcgcgtg tgcggagcgc ggcacctgtc ggagaacata aaaaatttcg tagacaaaca
      541 ggctgatgaa gacgctgatg gccgccagca cagctccgaa gactttgccg cgataaattt
      601 gtgactcgct gtacgcgcga acgagtccgg tggccaacaa aagtccaccc gcgcagaaca
      661 gcagaagatt gagccaggga agatcgcgcg tgattgggaa ttgcgcgagg gccggatagc
      721 tgaatacgcc gaggagaact aagacacagc caacccaaag tcgccagttc caccttccgt
      781 tcatatcgat cttgccgccg ccatccgcaa aatccaccga taaaggacga ttgcgatagc
      841 ggcaaaagcg acgccgccaa tgatgattgc ggtcgttgca aaccctgtgc cgaccagcgc
      901 cagcggcgca tctttgcccg agaacacgac gattgccgag atcacgacgc cgatgatgcc
      961 ctcgcccacg atcaagcctg acgcgagcaa cacgccaagt tgtttggccg agtcgggcct
     1021 cggcgtgcga tcagctcgcc gatcataaaa ccagccgaca atcgcaccca ccacaatcat
     1081 gagagtgctt tgcgtcggca gataaattcc cagacccacc gcgagcggcg ggatgcgcat
     1141 ctgtttggtc gtgcgcgcga ggatttcgtc cagcagaata atgccgacgc cgattgcgcc
     1201 gccgatccgg atcagactcc aatcgatgtc cgcggcgatc acgcctttgg ccaacgaaga
     1261 gattaacccg gcttgcggcg ccggtaatgg cgaagggcga gcttccgcac ctggcgcgcc
     1321 gacgaaacca taagcgtgat tcacgagatc gagaaccggc ggaatcacga acgcgccggc
     1381 gatgactccg atcacgagcg cccactgctg tttccacggt gtggcatcga cgagctggcc
     1441 ggtcttgagg tcttgcaagt tattgttcgc gatcgccgcc acgttgaaga ccaccgcggt
     1501 cgtgaaaagc gcgaacgcca tcagcgcttt gctggcatcg gggccaacgt acggttttac
     1561 gccaagcacc agcaacaagg ccgcgccgat tacaaccaaa attccgatgc ccgagagcgg
     1621 actattggac gaaccgatca accccgccat gtaaccacac actgccgaaa cgaaaaaact
     1681 catcaacacg atgtagacca cgccgccgat aatcagtgtt ggcaaatgcg cgcccagacc
     1741 gctggcgttt ccgaaatcgc cgagcaacca gccgattggc agcatgcatg ccaacgtggc
     1801 cagaccgacg atcccgatgg gaatgtcgcg ttcggtgatc ggcagcgtgt ccgctttgcc
     1861 cgccttacgt gcgcgcgaag cagccatcgc gctggtcagg cctcttacca ctggtttgat
     1921 caacttgagc aaagtccaaa tcgccgagac gccgattgcg ccggcgccaa cgaaacgcac
     1981 tttcgtgctc catgttcttg ttgcgagcct ggcgaccgcc gtcgtcatgt ccccagcgag
     2041 actcgaatag tgcggcacgc cccagcccca gccaatcaac gcaccgatga gcattgcgat
     2101 gccaactgag agaccgacta agtgtccaat gcccagaagc gcaaacgaca gaaagaagtc
     2161 gtaaccggtc acggcgccgc gcttgccgat tcgaaaagtc tgcgcgacat ccgcggcgaa
     2221 catttgtgtc gcgacgatga ccgcgaacac cgctgacacg atcgaacccc acaaaaccgc
     2281 gagcagcccg gcgcggccgt gctcgatatc atgagcgtga tcagaatggc cgctgctgcc
     2341 gaccttcagc acctccgcgc aggcgacacc ttcgggatag ggcagatccg aagtcgtcac
     2401 caatgcgcgc cgcagtggaa tggaatacat cacgccgaga attccgccga gcgcgcaaat
     2461 ccaaaacgaa gtccagaaag ggaactccgt ccagaaaccg atcatgatca aaccgggcaa
     2521 aacaaaaatg atcgaggaca gtgtgcccgc ggctgaggca accgtttgca caatgttgtt
     2581 ttcctgaatg gtcgcatccc gaaaaccgcg caagatcgcc atcgaaatca ccgccgctgg
     2641 aatcgatgtc gaaaaagtga gtccagcctt gagcccgaaa tacacattag ccgcggtgaa
     2701 cacgagcgtg atgatgacgc ctatgatgag accgcggaca gtgagttcct tgcgcatgtt
     2761 tgttcacgta caccgacgga cgcgagcgga gaatagtgtg gaaccgccaa acgggtcaag
     2821 gtggtgtgct gccttgtctg tgggctatga cgcgatgctc gcgccgagac acactcagtt
     2881 ataaccgtta cggcgccgca aaaatttgcc gccagcggca gtagacgaaa cgattcagtc
     2941 gatgcatttt acgagggcga ttgaggaatg gcgaagacac ggaaaacccg cagcgtgacc
     3001 aagcatgacc aaacgcaggc ccgtgagagc acgcaagaaa agctgcgggt aagcgaggaa
     3061 caattccggg tcctagtgga aagcgtagag gagtacgcga tctacctcct cgacccgcag
     3121 gggaacgtcg ctagttggaa tctgggcgcg gagaaactga agggctacac cgccgacgag
     3181 attatcggga aaaactttgc ctgtttttac accgacga
//
LOCUS       DASTDG010000161         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1041765, whole genome shotgun sequence.
ACCESSION   DASTDG010000161 DASTDG010000000
VERSION     DASTDG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041765"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFO30_05370"
     CDS             complement(<1..763)
                     /locus_tag="VFO30_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9418751.1"
                     /translation="MADIPKTYDPKSVEPKWYAQWIENRDFEANSKSPKPPFSIVMPP
                     PNITGVLTLGHVLNNTIQDILARRARMLGFEVLWLPGVDHAGIGTQTAVEKWLRKKEG
                     VTRHDLGREEFLRRVVEWQDKHGGIIIEQLKRLGCSADWSRQRYTLDDAYVRAVQTVF
                     VDLYHKGLIYRGRRMINWDPAAQTALSDEEVINKPQRGNLYYVRYEIVDESSATVPLA
                     TLKAQAGGSRHFLEVATTRPETIMADTGVAVHPRDQ"
     gene            860..1678
                     /gene="murI"
                     /locus_tag="VFO30_05375"
     CDS             860..1678
                     /gene="murI"
                     /locus_tag="VFO30_05375"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9418752.1"
                     /translation="MSDSRPIGVFDSGIGGLTVAKALLEVLPNERILYLGDTARVPYG
                     GKSAETVQRYSLELADILLRENAKAIVVACNTVSSVALPQLRKRFDVPVVGVLEPGAR
                     AAVAATRNRHIGVIGTRATIRSGAYETALRGLDADLQITSRACPLLVPLIEEGMLDDP
                     VTDQVIARYLEPMLRDGIDTLVLGCTHYPLLAAAIARSLGENIRLVDSAVNCARSVSE
                     LLDRESLHAAIGNSGSLEVSLTDSPDNFLRVAREALQLDIGEVEVREVLHPAKS"
     gene            complement(1806..3167)
                     /locus_tag="VFO30_05380"
     CDS             complement(1806..3167)
                     /locus_tag="VFO30_05380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET9418753.1"
                     /translation="MTTPSDSQRMEKIVSLCKRRGFIFQSSEIYGGLNGLWDYGPLGV
                     ELKRNLKNYWWRVMVHERNDVVGMDGSILMNRAVWKASGHEDTFTDPMIDCRSCKARL
                     RGDQLIEKKGAKQCPVCGSKDLTEPRAFNLMFKTYVGATEDDSSVTYLRPETAQAIFV
                     QFKNVLETSRKKLPFGIAQIGKAFRNEINPRNFTFRSREFEQMELEYFCRPEQGMELL
                     EYWKDERLRFYDNIGIAREHLHMRTVADEERAFYSKGTYDIEYDFPFGRQELEGVAYR
                     TDYDLVQHQNASGKSLEYFDEETKQRFIPHVVEPSAGVDRTVLALICDAYSEDEAPDD
                     RGKMETRTVLRFHPRVAPIKCGVFPLLKNKEQLVAKAKEVVDLLRPLCFVFYDESGAI
                     GRRYRRQDEIGTPFCVTIDFETLGERDRQLQDTVTLRERDSMKQARVKISELKELLAA
                     RIA"
BASE COUNT          664 a         1035 c          979 g          774 t
ORIGIN      
        1 gctggtcgcg cggatgaacg gcgacgccag tgtccgccat gatcgtctct ggccgtgtcg
       61 tcgcgacttc gagaaagtgg cgcgagcctc cggcttgcgc ttttaaagtc gcaagcggga
      121 cggtcgcgct actctcgtcc acaatctcgt agcgcacgta atagagattc ccccgctgcg
      181 gtttgttgat cacctcctcg tccgagagcg cggtttgcgc cgccgggtcc cagttgatca
      241 tccgccggcc gcggtagatc aggcctttgt ggtaaagatc gacaaaaaca gtttgcaccg
      301 cgcgaacgta agcgtcgtcg agcgtgtagc gctggcgcga ccaatcggcc gagcaaccga
      361 gccgcttgag ttgctcaatg atgatgccgc catgtttgtc ctgccattcg acaacgcgac
      421 gcagaaattc ttcccggccg agatcgtgcc gcgtcacacc ttcctttttg cgcagccact
      481 tttcgaccgc cgtttgcgtg ccgatgccgg catgatcgac acccggcagc cagagcactt
      541 cgaaaccaag catgcgcgcg cggcgggcga gaatgtcctg gatggtgttg ttgagcacgt
      601 gccccagcgt caacacgccg gtaatattcg gtggcggcat cacgatggag aacggcggct
      661 tcggcgattt cgagttcgct tcgaaatcgc ggttctcgat ccactgcgcg taccactttg
      721 gctcgactga ttttggatcg taagttttcg gaatgtccgc catcgcaaaa aagtagcgca
      781 agcctctggc ttgcgagccg taacaaacgc aagccggagg cttgcgctac tctattcgct
      841 cactcacctt ccccgcagca tgagcgactc gcggccgatc ggcgtcttcg actccggcat
      901 cggcgggttg acggtggcga aggcgttgct cgaagttttg cctaacgaac ggattcttta
      961 cctcggcgac accgcgcgcg tcccgtacgg cggcaaaagt gcagaaacgg tgcagcgcta
     1021 cagtctcgag ctcgccgaca ttctcttgcg cgaaaacgcg aaggcgatcg tcgtcgcgtg
     1081 taacacggtt tcttcggtgg cacttccaca actgcgcaag cgtttcgatg ttccggtggt
     1141 cggcgtgctt gagcccggcg cacgcgcggc cgtggcagcg acgcgcaacc gccacatcgg
     1201 cgtgatcggc acgcgcgcca cgattcgcag cggcgcgtac gaaacggcgc tgcgcggact
     1261 cgatgccgat cttcagatca cgagtcgtgc ctgcccgctg ctcgtgccac tgattgaaga
     1321 aggcatgctc gacgacccgg tgaccgatca agtgatcgcg cgctatctcg aaccgatgtt
     1381 gcgcgacggc atcgatacgc tcgtcctggg ttgcacacat tacccgttgc ttgccgcggc
     1441 gattgcgcgc tcgttaggcg aaaacattcg gctggttgat tcggccgtga actgcgcgcg
     1501 gtccgttagt gagttattgg atcgagagtc attacatgct gcgatcggaa actccggtag
     1561 ccttgaagtc agcctgactg attcgccgga caatttcctg cgcgtcgcgc gcgaagcgct
     1621 gcaactcgat atcggagagg tggaggtacg cgaagtttta cacccggcca aatcgtagag
     1681 gcgcttggca caagcggctg atacagcccg gttcttcaat tcgccactta ctcctcgatt
     1741 ttctccaaat cagaaatcgc cggcgcgttc gcccgacaac gtttgcgatt gccatctcaa
     1801 tgaaactacg cgattcgcgc cgcgagtagt tcctttagct cggaaatttt cacgcgcgcc
     1861 tgtttcatgg aatcgcgctc ccgcaacgtg accgtgtctt gcagttgtcg atctctctcg
     1921 ccgagggttt cgaaatcgat ggtgacgcag aatggcgtgc caatttcatc ctgccgccga
     1981 tagcggcggc cgatcgcgcc gctctcgtcg taaaacacga agcaaagcgg ccggagaaga
     2041 tcgacaacct ccttcgcctt cgcgacgagc tgctccttgt ttttcagcaa cggaaagacg
     2101 ccgcatttga tgggcgcgac gcgcgggtgg aaacgcaaga ccgtgcgcgt ttccattttg
     2161 cctctatcat ctggcgcttc gtcttccgag taagcgtcac aaatcagcgc gagaacggtg
     2221 cggtcaaccc ctgcgctcgg ctcgacgacg tgcgggatga agcgctgctt ggtttcttca
     2281 tcgaaatact caagcgattt gccgctcgcg ttttggtgct gcacaaggtc gtaatcggtc
     2341 cgataagcga cgccttcgag ctcctgtcga ccgaacggga aatcgtattc gatatcgtaa
     2401 gtgcctttgg aataaaacgc gcgctcctcg tccgcgaccg tcctcatgtg caagtgctcg
     2461 cgcgcgatgc cgatgttgtc gtagaagcgc aaccgttcgt ccttccaata ttcgagcagc
     2521 tccatgccct gctctggccg gcagaaatat tccagttcca tctgttcgaa ctcgcgggag
     2581 cgaaacgtga aattgcgcgg attaatttcg ttacggaagg ctttgccgat ttgtgcaatg
     2641 ccaaacggca acttcttgcg cgatgtttcc agcacatttt tgaactgcac gaaaatcgcc
     2701 tgcgccgtct ccggccgcag atacgtgaca ctcgagtcgt cctccgtcgc gccgacgtag
     2761 gtcttgaaca tcaagttgaa cgcgcgtggc tcggtcaggt ctttgctccc gcagaccgga
     2821 cattgcttgg cgcctttttt ttcgatgagc tgatcaccgc gcagacgcgc cttgcaagat
     2881 cgacaatcga tcatcggatc ggtgaaggtg tcctcgtgac cgctcgcctt ccaaaccgcg
     2941 cgattcatca ggatcgagcc gtccatgccg acgacgtcgt tgcgttcgtg taccatgacg
     3001 cgccaccaat agttttttag attgcgcttc agctcgacgc cgagcgggcc gtagtcccac
     3061 agcccattca ggccgccgta aatttcgctc gactgaaaga tgaagccgcg ccgcttacac
     3121 aagctgacga tcttctccat ccgctgtgag tcagacggcg tggtcatgtt gcgcacagat
     3181 tagcgtagaa tattctgatc aaaagcggtt gtagaggcgc ctgtgccaag cgcctgttcc
     3241 gaaattcggc gtttgacaca aacgccgcta caacgttcac gatttaactt tgcggctcga
     3301 ccgtgatttc ggttttgacc gagttggcga tgaaacactg ttcgtgcgcc gcgtgattca
     3361 tctcgtcgag atcctgccgc gacggctgcc gctcgccgga gaattttgtc cgctggtgga
     3421 gagtcacgcg cgtaatcgcc atcctgcctt cc
//
LOCUS       DASTDG010000162         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1627379, whole genome shotgun sequence.
ACCESSION   DASTDG010000162 DASTDG010000000
VERSION     DASTDG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627379"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFO30_05385"
     CDS             <1..792
                     /locus_tag="VFO30_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET9418754.1"
                     /translation="DAGRYFLRKQKWSEGASQEELIAIAVRSLGLSELKPFEPMDKVI
                     ELKIESANPKPSLVKMSLREFCNETLSDSPAPGGGSVAALLGALGASLGGMVANLSAG
                     KRGWDEKLQYFSEWAVKAQQLKDELLFLVDEDTAAFKKVIDAFALPKGSDEEKSARFS
                     AIEAANKYAAEVPLRVMETAARSYAVLADMAQNGNPASISDVGVGALATRACINGAAL
                     NVRINLAQLKDEKAKSVLLKKAQQIATESDAQFKKIQQAVEAKLA"
     gene            866..>2039
                     /gene="hutI"
                     /locus_tag="VFO30_05390"
     CDS             866..>2039
                     /gene="hutI"
                     /locus_tag="VFO30_05390"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET9418755.1"
                     /translation="MSSLAILNASQLVTLAGPRRPRVGREMSDLGIIAKGGVLIGDGK
                     IDSVGISDEIEKKARGAEIVDVAGRVVLPGFVDAHTHLIFAGNRLEDFERRARGETYE
                     QIAKAGGGIWSTVEETRAASETELFEQAKKRAEWFLGGGTTMVEAKSGYGLTIEDELK
                     ILRVIRRLNAETPLEMVPTFLGAHAVPRGTSADEYVDLVINEMLPRVAREELAEFCDV
                     FCERAYFDLENSRRILDAAKKLGLKLRGHVDQLSNFGGAKLMAQLSAATADHLEKTDE
                     QGIAALKAGNVQPVLLPGSVYALGSTSYPRARDMIEAGLALVLATDFNPGSSPTPSMP
                     MILSLACTQMKMSPAEAMTASTTNAAYSLGRGDRVGSLEPGKLANFSVVDCADYREL"
BASE COUNT          439 a          526 c          663 g          411 t
ORIGIN      
        1 gatgctgggc gctacttttt gcggaagcaa aaatggtctg aaggcgcatc gcaggaagaa
       61 ttgattgcca ttgcggtgcg atcgctcgga ctcagcgagc tgaaaccttt cgagccgatg
      121 gacaaggtga ttgaattgaa aatcgagtcg gcgaatccga aaccgtcgct tgtgaaaatg
      181 agccttcgcg agttctgcaa cgaaacgcta agcgattcac ccgcgccggg cggcgggtcg
      241 gttgccgcgc tactgggcgc actgggtgcg tcattaggcg gaatggttgc gaatctctct
      301 gcgggcaaac gcggctggga tgagaagctc cagtatttta gtgagtgggc agttaaggcg
      361 cagcaattga aggacgagtt gttgttcttg gtcgacgaag acacggccgc tttcaaaaag
      421 gtcatagacg cattcgcctt gccgaaagga tcagacgaag agaagtcggc gcgtttctcc
      481 gcgattgagg cagcgaacaa gtacgccgcc gaggttccgc tgcgggtaat ggaaacggcg
      541 gcgagatcct acgcggttct tgccgatatg gcgcagaacg gaaaccccgc ttcaatttct
      601 gacgtgggcg tcggcgcgct tgccacgcgc gcctgcatca acggagcggc cttaaacgtg
      661 cggatcaacc ttgcgcaact taaagatgag aaagcgaaat cagtgctctt gaagaaagcg
      721 cagcagatcg ccaccgagtc tgacgctcag ttcaaaaaaa ttcagcaagc tgtggaagcg
      781 aaactagctt gaattctgat ggagagcgtc cgcgccgtcg gcgccgttac attcgggaca
      841 cgacggcgcg tgtccctgcc gattggtgag ctcgctcgcg attctcaacg cctcgcagct
      901 cgtcacgctg gctgggccac ggcgcccacg cgtcggccgc gagatgtcgg atctcggaat
      961 cattgccaaa ggcggggtgc tcatcggcga cgggaagatc gacagcgtcg gcatcagcga
     1021 cgaaattgag aagaaggcgc gcggcgcgga gattgttgat gttgcaggac gtgtcgtgtt
     1081 gccgggattt gtcgatgcgc acactcattt gatctttgcc gggaatcggc tggaggattt
     1141 cgagcgccgc gcgcgcggtg agacttacga gcaaatcgcc aaagctggcg gtggaatctg
     1201 gtcgacggtt gaggagacgc gcgccgcgag cgagaccgaa ctgttcgagc aagcgaagaa
     1261 gcgcgctgaa tggtttctag gcggcggcac gacaatggtt gaagcgaagt cgggttacgg
     1321 tctcaccatc gaggacgagt tgaagattct gcgtgtgata cggcgactga acgcggagac
     1381 gccgctcgaa atggtgccga catttcttgg cgcgcatgcc gtgccgcgcg gcacttcggc
     1441 cgatgaatat gtcgatcttg tgatcaatga aatgttgccg cgcgtcgcgc gggaagaact
     1501 ggcggaattc tgcgatgtgt tttgcgagcg cgcctatttc gatcttgaga actcgcgccg
     1561 aatcttggac gcggcgaaga agctcggttt aaaactacgc gggcacgttg atcagctatc
     1621 gaacttcggc ggagcgaagt tgatggcgca gcttagcgcg gcgacggcgg atcatttgga
     1681 gaaaacagac gagcagggga tcgcggcatt gaaagcggga aatgtgcagc cggttctgtt
     1741 accaggctct gtttatgcgc ttggatcgac atcgtatccc cgtgcgcgag atatgatcga
     1801 ggcgggcctt gcacttgtgc tcgccaccga cttcaacccg ggttcgtcgc cgacgccatc
     1861 gatgccgatg attctctcgc tcgcgtgcac ccagatgaaa atgtcgccgg cggaagcgat
     1921 gaccgcatcg acaaccaatg cggcctattc gttaggccgc ggcgatcgcg tcggctcgct
     1981 cgagccgggg aagctcgcga atttttcggt cgtcgattgc gccgattatc gcgaactcg
//
LOCUS       DASTDG010000163         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1250397, whole genome shotgun sequence.
ACCESSION   DASTDG010000163 DASTDG010000000
VERSION     DASTDG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250397"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1436
                     /locus_tag="VFO30_05395"
     CDS             69..1436
                     /locus_tag="VFO30_05395"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HET9418756.1"
                     /translation="MPEIQMPKLSDTMTEGTLVAWRKKKGDKVSAGDVLAEIETDKAT
                     MEWESPEDGTLTDIYVEEGGKVNVGEKIAFIGAHGEEAPKKEEPPKKAEAIEEKKPQA
                     ETEKPAPAEKKEKETAPPEEEKEKPAEPAKKSVEPSVTAVKERPTRPPLQEKGEEARV
                     KASPVARRVAAELGIDLATVKGTGPEGRVTETDVRAAAKAQTVEAGVPPAPKQPARLP
                     PQPLQKREGARIQLSGMRKGIAERLIQSKAPVPHFYLNIEINAGPLMEARGELKAAGE
                     ESEEAKITVNDFVLKAAVMAAVKVPKANASFDGDSIVQFADIDLGIAVAIEDGLLTPV
                     IRAAQNKSLREISALAKDLATRARSKRMKPEEFQGGTFTVSNLGGMGIDSFSAIINPP
                     QGFILAVGKIDKVPVVDDCDQIVVGHRLSIWMSCDHRVIDGALGAQYLKELRHLLENP
                     ALLLV"
     gene            complement(1441..1833)
                     /locus_tag="VFO30_05400"
     CDS             complement(1441..1833)
                     /locus_tag="VFO30_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET9418757.1"
                     /translation="MHSKLEVEFAHALSSAKAYVRNPERLRDLVTEGTRKALFLPHET
                     FKGTLVYLHAMLRLMRAYYRGEYRAVPMTTLLIIVAALIYLLNPLDMIPDWIPGLGFI
                     DDAFILTLAARRTREVIEQFLAWESAHP"
     gene            complement(1919..>3079)
                     /locus_tag="VFO30_05405"
     CDS             complement(1919..>3079)
                     /locus_tag="VFO30_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9418758.1"
                     /translation="EAFRSAICLASGVARAVALITCRCYASKNKANMPRKSRRKKQVT
                     PERLMQFGFAYAPPLIIGAAVNNQVFDTLESGAKTVDQVSNQTAASRRGLSAIMNALV
                     GLELLKKDRSAAGRIRRGEQARYSLTPESAAFLVSNKPGSLAGFFAMNATHLIPDWLH
                     LSEVVRTGAPAIALNQESRGPEFFSVLVENIIPMSYPAAMKLGESLRLSKAKTPVRVL
                     DVAAGSGIWGIALAQQSPQVQVTAQDWTEMIPTTKRITQKFGVAGQFSYVAGDVLKAD
                     FGRDYDIATLGHILHTEGEERSRELLKRVFRALKPGGTIAVAEWLVDDKRTAPLNGLI
                     FAVNMLVHSEHGDTFSFREIKSWLQETGFEKVRKLDAPGPSPLVLATKPPHA"
BASE COUNT          695 a          855 c          949 g          580 t
ORIGIN      
        1 ctgcggtaaa aaaagtgtgt tacgcgtgaa tctcgtcact ggtcacttgt cactcgtcac
       61 tttcgcttat gcctgaaatt cagatgccca agctcagcga cacgatgact gagggcacac
      121 tcgtcgcgtg gcggaagaag aaaggcgaca aagtttctgc cggcgacgtg ttggcggaga
      181 tcgagaccga caaggcgacg atggaatggg agtcgcccga ggacggcacg ctcaccgaca
      241 tctacgtcga ggaaggcggc aaggtgaacg tcggcgagaa gatcgcgttc atcggcgcgc
      301 atggcgaaga agctccgaag aaggaagaac cgccgaaaaa agccgaagca atagaagaaa
      361 agaaaccgca agccgaaacc gagaaacctg caccggctga aaagaaagaa aaagaaactg
      421 cgccgccgga agaagagaaa gaaaaacccg cagagccggc caagaaaagt gtcgagccaa
      481 gcgtgacggc tgtaaaagag cggccgacac ggccgccgct acaggaaaaa ggtgaagaag
      541 cgcgggtcaa agcgtccccg gttgcgcgca gagtcgcagc cgagctcggg atcgatcttg
      601 cgaccgtgaa aggaaccgga cctgaaggcc gcgtcaccga gaccgatgtg cgcgccgccg
      661 cgaaggcaca aactgtagag gcgggcgtgc cgcctgcacc aaagcagccg gcacggctgc
      721 caccacagcc actacagaaa cgcgaaggcg cgcgcatcca gctttccggc atgcgcaaag
      781 gcattgccga gcgtctcatt caaagcaaag cgccggttcc gcatttttat ctcaacatcg
      841 aaatcaatgc aggcccgctg atggaagcgc gcggggaatt gaaagccgcc ggtgaagaat
      901 cggaagaagc gaaaattacg gttaacgatt tcgtgttgaa agcggcagtg atggccgcgg
      961 taaaagtgcc gaaggcaaac gcgtcattcg acggcgattc gattgtgcaa tttgccgata
     1021 tcgatcttgg catcgcggtt gcgattgaag acggcttgct cacgccggtc attcgcgcgg
     1081 cgcagaacaa atcgctgcga gagatcagtg cattggcgaa agatctcgcc acgcgcgccc
     1141 gcagcaagcg aatgaaaccg gaagaatttc aaggcggcac cttcaccgtc tcgaatctcg
     1201 gcggcatggg tatcgattcc ttcagcgcga tcatcaatcc gccgcaaggc ttcattctcg
     1261 cagtgggcaa gatagacaaa gtgccggtgg tcgatgattg cgaccagatc gtcgttggcc
     1321 atcgcctgtc gatctggatg agctgcgacc accgcgtcat cgacggcgcg ctcggcgcgc
     1381 aatacctaaa agaactccgc cacctgctcg agaaccctgc gctgctcctc gtctaacaaa
     1441 ttaggggtgg gcggattccc acgccaggaa ctgctcgata acttcgcgcg tgcggcgggc
     1501 agcgagcgtg aggatgaacg cgtcgtcaat gaagccaagg ccggggatcc agtctggaat
     1561 catgtcgagc gggttcagga ggtagatgag cgcggcgacg atgatgagca gcgtcgtcat
     1621 tggaaccgcg cgatactcgc cgcgatagta ggcgcgcatc aatcgcagca tcgcgtgaag
     1681 gtaaacgagc gtgcccttga aggtctcgtg aggtagaaag agcgccttcc gcgtcccttc
     1741 ggtcacgaga tcgcgcaggc gctcgggatt tctgacgtaa gcctttgcgc tgctgagcgc
     1801 atgcgcaaac tctacctcca gcttcgagtg caagggcgaa tcgatcggcg cagtcgcttt
     1861 cggttttggc tgccatcgat gtgaccgcgc gactgaacgc gccgctcttc gttttcgatc
     1921 aggcatgtgg tggtttggtg gcgagcacaa gcggggacgg cccgggcgcg tcgagtttcc
     1981 gaactttctc gaagccggtt tcttgcagcc agcttttgat ctctcgaaag ctgaaggtgt
     2041 cgccgtgctc actgtggacg agcatgttga ccgcgaagat caacccgttc agcggagccg
     2101 tgcgtttgtc atcgacaagc cactcggcga ccgcgatcgt gccgccgggt ttgagcgcgc
     2161 gaaagactcg cttaagcaac tcgcggctgc gctcctcacc ctcggtgtgg aggatatggc
     2221 cgagcgtcgc gatgtcatag tcacgaccga aatcagcttt tagtacgtcg cccgcgacgt
     2281 agctgaattg accggccacg ccgaattttt gcgtaatccg cttcgttgtc gggatcatct
     2341 cggtccaatc ttgcgcggtc acctgcacct gtggcgactg ctgcgcgagc gcgatgcccc
     2401 agatgccgga gccggcggcg acgtcgagca cgcgcaccgg cgttttcgct ttcgataacc
     2461 gcagcgactc gcccagcttc attgcggccg gataactcat cggaatgatg ttctcgacca
     2521 gcacggagaa aaattccggg cctcggcttt cctgattgag cgcgatggct ggtgcgccgg
     2581 ttctcactac ttcagacaag tgcaaccagt cgggaatcag gtgtgtcgcg ttcatcgcga
     2641 aaaatccagc gagcgaacct ggcttgttgc tgacgaggaa ggcggcgctt tcgggcgtga
     2701 gggaataccg cgcttgttcg ccacggcgaa tccgtcctgc ggcggatcga tctttcttga
     2761 gcagctcgag gccgacgagc gcgttcatga tcgcgcttaa gccgcgccgt gatgcggcgg
     2821 tttgattact gacttggtcg acggttttcg cgccgctctc gagtgtgtcg aagacttggt
     2881 tgtttaccgc tgcaccaata atgagaggcg gcgcgtaagc gaacccgaat tgcatgagcc
     2941 gttcgggtgt gacctgcttt ttcctgcggg attttctggg catatttgct ttgttttttg
     3001 aagcgtaaca tcgacaggta atcagcgcga cggcgcgagc aactccggat gccaaacaaa
     3061 tcgccgaacg aaaagcctc
//
LOCUS       DASTDG010000164         7192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1493163, whole genome shotgun sequence.
ACCESSION   DASTDG010000164 DASTDG010000000
VERSION     DASTDG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493163"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="nrfD"
                     /locus_tag="VFO30_05410"
     CDS             <1..1432
                     /gene="nrfD"
                     /locus_tag="VFO30_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9418759.1"
                     /translation="IRAEASTTVEEEVKRPPLVLHNRNLSWITERISGVVEQRAPRWW
                     WVTFTITAGIAAFGLFCLGYQISTGVGVWGLNHPVGWAWDITNFVFWIGIGHAGTLIS
                     AILYLLRQKWRTSINRSAEAMTLFAVMCAAIFPGIHVGRVWMAWYLAPLPNNWAIWPN
                     FRSPLMWDVFAVSTYFTVSVLFWYTGLVPDLATLRDRATSKIKKFLFGVFALGWRGSN
                     RNWRHYEMAYLLLAGLSTPLVLSVHSVVSFDFATSILPTWHSTIFPPYFVAGAIFGGF
                     AMVLTLLIPARAMFKLHDIITPQHIDKMAKIILLTGSFVGYAYTMEFFVAWYSGNSYE
                     RFAFWNRVFGPYWWYWWVGMLFCNVLSPQLFWFRWCRRNIFVVYFVCMCVNAGMWFER
                     FIIIVGGLHRDFLPSAWGMFHATWVDMWTFIGTLGIFTSLFLLFVRFLPMIAMSEVKI
                     VVPEADPHYVSPPVHHPIAAGGKERT"
     gene            1551..2105
                     /locus_tag="VFO30_05415"
     CDS             1551..2105
                     /locus_tag="VFO30_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9418760.1"
                     /translation="MRTPSGHKVYAMAAEYPSAHALYEAAKMVRDAGFKRWDVYSPFP
                     IHGMDDAMGLGKSWLSAVVLVGGISGLLTAMIVEFGPSWILYPLIVHGKPYTWRTVPA
                     FFPIMFELTVLFSAFAAFFAWQIMNGLPRWNHPMFNWQRFNRATNDGFFLAIEARDPR
                     FTETEARELLERSGGEHITIVHEE"
     gene            2109..2846
                     /locus_tag="VFO30_05420"
     CDS             2109..2846
                     /locus_tag="VFO30_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9418761.1"
                     /translation="MLRGFLLLFLLLTIALLAVLGFRGQKSTGPPLEVFPDMVRQMKV
                     RAQAPLEFFADGRGPRMPIGGTVPIGYEMPQAKTIATPTTALPVPQTRSQLAFSASTD
                     YHDTGKMGGNWGTGIPMQVTPDLLGRGQQRFNITCAMCHGATATGNGITKQYGLTTVV
                     SLQDDRIRKMADGEIFNTITNGKNTMMAYGPTITVDDRWAIIAYLRALQRSHAATVAD
                     VPPEHRADLDKPAESPPPPGKPEAQKK"
     gene            2843..4045
                     /locus_tag="VFO30_05425"
     CDS             2843..4045
                     /locus_tag="VFO30_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418762.1"
                     /translation="MSDRPHDLPMPQGEYFETNRFAGLSMLLGVIAFVALALCVVGAI
                     VDPTQLGFSWLFAFAFFFTLCAGCFFWTIVHHATDAEWSVVVRRQLENIALLMFVLAL
                     FFIPILLLRHHLYEWMNIPRGQEENLDSKRAYLNWTFFVVRAVIFLGFFIVATFLLRR
                     FSVRQDKDGNPRFTILMRKVAFASLPMFALCLTFGAFDWLMSLNYRWFSTMFGVYIFA
                     GTAGASMSLLVLVITALRNAGYLKDVVTMEHYHIMGKWMFAFCTFWAYIGFGQYMLIW
                     YANMPEETQYFLVRTTQSWWGLSMLLVFGRFFGPFAILLLRSIKKHPRQLCYVAGWIL
                     LMQMLDMYLVVLPALHGTGVHLSLWDFVTVIAFGATLAFVYLRIVGKTSLFPVRDPRL
                     VESLRLVN"
     gene            4047..4700
                     /locus_tag="VFO30_05430"
     CDS             4047..4700
                     /locus_tag="VFO30_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418763.1"
                     /translation="MADAESIRQAVRARAPFSTWLGVIALFFLFGVIVLAVVGPAPRG
                     DTYEEMRARKRIDNLKAAREADAKELGTYGWIDKNKGTVRVPIDRAMQLAVAELAKQK
                     PAPAYAIAPPAPQASQAPAGAATPAAPALPTPQPSATPKPISVAGPESENRAQPAAAA
                     NPPGAQPGTQPGASASPAASPASSTAQPAVPPSPRPAPSTPPGSPLPVRGKTPPPGQ"
     gene            4741..6228
                     /locus_tag="VFO30_05435"
     CDS             4741..6228
                     /locus_tag="VFO30_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9418764.1"
                     /translation="MDATEIPLKAPELPTHAPVTETEQFERALIDASTRVPVLMFYAS
                     AIFWLLAGTILGGLVSWKLHSPDLLSGVSFLTWGRLRPVHMNIMVYGWASMAAMGTGV
                     WIMARLCRTVLRRPLVIVAGAGFWNLGVLMGVCGILLGDSTGYQWLEFPPYASIVLFA
                     AYTLMLSWAVLMFRFRRGEQIYITQWYLLGAFLWFPWLYAAAQLMLFVVPVQGVMQAA
                     VTWWYANNVMFLWLGAIALGTAYYMIPKVIGRPVYSYHLATIGFWTYALFSSWTGMQR
                     LVDGPFPAWMITASIAATILTLIPVATVGLNHHMTMLGYFPLLRYSPTLRFTVFGAMS
                     YTVFSGVGILISLRSIARYVNFSEASIGYSHLGLYAFVTMILFGSMYYIVPRLVGREW
                     RYATLIKLHFWSSAYGIGLMTLMLLAGGFLQGANIDNPSLEFTESVQTILPYLRGRSL
                     SGILLTVSHFVFAYHFGLMLFGLGRTSSVPTFLNPVEAEGSEEHL"
     gene            6225..>7192
                     /locus_tag="VFO30_05440"
     CDS             6225..>7192
                     /locus_tag="VFO30_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HET9418765.1"
                     /translation="MKGLASLILGIFGTFSFSWLGLILVPNYQIGHLNPQMDEEGTDV
                     YPTPKSGMAERGRRVYVANGCYYCHSEQVRPDYAAGDIDRKWGDRRSAPRDYIFDRPV
                     LLGKERMGPDLSNVGKRAPAEGEAPNAANAAITAAPGGGSPAPPAPPAGGAPAQPKTP
                     PAGSPAQSPAAAPQTPPNAPARPAASPPAQNPPPAAAGSPAQSPAATSAPAAANAPPP
                     PPAAALSPSVNPPTGEPLPYSAAWHHRHLYAPRSVTLDSNMPAFRFLYEKRPSGSEPA
                     ANAVQLTATDAPPVGWEVVPTYDAECLVAYLMSLDQSHPLKEVKSAG"
BASE COUNT         1270 a         2212 c         2087 g         1623 t
ORIGIN      
        1 aattcgggcg gaagcgtcga cgacggtcga ggaggaggtc aaacgtccgc cgctggtttt
       61 gcataatcgc aacctcagct ggatcacgga gcgcatttcc ggggtggtcg agcaacgggc
      121 gccgcgctgg tggtgggtga catttacgat caccgctggc atcgcggcgt ttggattgtt
      181 ctgcctcggt taccagattt ccaccggcgt cggcgtgtgg ggattgaatc acccggtcgg
      241 ttgggcgtgg gacattacca acttcgtgtt ctggattggt atcggacacg ccggcaccct
      301 catttcggcg atcttgtatc tgctgcgcca gaaatggcgt acctcgatca accgttcggc
      361 cgaagcgatg acgctgtttg cggtgatgtg cgccgcgatt ttccctggca ttcatgtcgg
      421 acgcgtctgg atggcgtggt atctggcgcc cttacctaac aactgggcga tttggccaaa
      481 cttccgcagc ccgctcatgt gggatgtctt cgcggtctcg acctatttca ccgtgtctgt
      541 gttgttctgg tacacgggac tcgtgccgga tctcgcgacc ctgcgtgatc gcgcgacttc
      601 gaagatcaaa aagttcttgt tcggcgtctt cgccctgggc tggcgcgggt cgaaccgcaa
      661 ttggcgtcat tatgagatgg cgtatctgct tctcgccgga ctgtcgacgc cgctcgtgct
      721 ctcggttcac agcgtcgtgt cattcgactt cgcgacatcc atcctgccca catggcattc
      781 gacaatcttc ccgccttact tcgtcgccgg cgcgatcttc ggcgggttcg ccatggtgct
      841 gacattgctc attcccgcgc gggcgatgtt caagctgcat gacatcatca cgccgcagca
      901 catcgacaag atggcgaaga tcattctgct gaccggctcg ttcgtcggct atgcctacac
      961 gatggaattt tttgtcgcct ggtatagcgg caacagttac gagcgattcg cgttttggaa
     1021 tcgcgtcttt ggtccgtact ggtggtattg gtgggtcggc atgttgttct gcaacgtgct
     1081 ttcgccgcaa ctgttctggt tccgctggtg ccggcgcaac atcttcgtcg tctacttcgt
     1141 ctgcatgtgc gtgaatgccg ggatgtggtt cgagcgattc atcatcattg tcggaggatt
     1201 acaccgcgat ttcttgccgt cggcgtgggg aatgtttcac gcgacctggg tcgatatgtg
     1261 gacgttcatc ggcacgctcg gcatcttcac ttcgctgttc cttctgttcg ttaggtttct
     1321 gccgatgatc gccatgagcg aagtgaaaat cgtcgttcca gaggccgacc cgcattacgt
     1381 cagcccgccc gtccatcatc caatcgcagc cggaggaaag gagcgcacat gagaacgcga
     1441 cgcctttgct gtagccacgg ctctgcgggc cgtctggcgt tgcatgtccg cgcgttgttt
     1501 gaaccggcca ctggccagtg gctacaaaag cacatcggaa aggagcgcac gtgagaacgc
     1561 cttcgggtca caaagtttac gcgatggcgg cggagtaccc gagcgcgcac gcgctctacg
     1621 aagccgccaa gatggtgcgc gacgccggat tcaaacgctg ggatgtgtac tcgccattcc
     1681 cgattcacgg aatggacgac gcgatggggc tcggcaaatc gtggttgagc gcagtcgttt
     1741 tggtcggcgg cataagcggc ttgctcacag cgatgattgt ggagtttggc ccgtcatgga
     1801 ttctttatcc gcttatcgtc catggtaaac cgtacacctg gcgcacggtc ccggcatttt
     1861 tcccaatcat gttcgagctg accgtgctct tctccgcgtt cgccgcgttt ttcgcctggc
     1921 aaatcatgaa cggcctgccg cggtggaacc atccgatgtt caactggcaa cggttcaacc
     1981 gcgctacgaa cgacggtttc tttctcgcaa tcgaggcgcg cgacccgcgc ttcaccgaaa
     2041 cagaagcgcg cgagttgctc gagcgcagcg gcggcgaaca catcaccatc gtgcacgagg
     2101 agtgatttat gttgcgcggc tttctcctgc tcttcttgct gctgaccatc gccctcctcg
     2161 cggtgctcgg tttccgcggg caaaaaagta ccgggccgcc gctcgaggtt ttcccggaca
     2221 tggtgcgtca gatgaaagtg cgcgcgcagg cgccgttgga attttttgca gacggccgcg
     2281 gaccgcgcat gccgatcggc ggcacggtcc cgatcggcta cgaaatgccg caggcaaaaa
     2341 caatcgccac accgacgacg gcgctgcccg ttccacaaac gcgctcgcaa ctcgcgttca
     2401 gcgcgagcac cgattatcac gacactggga aaatgggcgg caactggggc acgggaattc
     2461 cgatgcaagt cacgcctgat ctcctgggac gtggtcaaca acgtttcaac atcacctgcg
     2521 cgatgtgtca cggcgcgacg gccaccggga acggcatcac gaaacagtac ggcctaacga
     2581 ctgtggtcag cctccaggac gaccgcatcc gaaaaatggc tgatggcgaa atttttaaca
     2641 ccatcacgaa cggcaagaac acgatgatgg cgtacgggcc aaccatcacg gtcgatgatc
     2701 ggtgggctat cattgcgtat ttgcgcgctc tgcagcgcag tcacgccgcg accgtggcgg
     2761 acgtgccgcc ggagcatcgt gctgatttag ataaaccggc tgaatcgcca ccgccgcccg
     2821 gcaaacctga ggcgcagaaa aaatgagcga tcgcccacac gacctgccga tgccgcaagg
     2881 cgaatacttc gagacgaacc gcttcgcggg attgtcgatg ttgttaggcg tgatcgcgtt
     2941 cgtcgccctc gcgctctgcg ttgtcggagc gattgtcgat ccaacgcagc tcggtttttc
     3001 ctggctgttc gcgtttgcgt tcttctttac cctgtgtgcc ggctgcttct tctggacaat
     3061 tgtccaccac gcgactgatg cggagtggtc ggtggttgtg cgtcggcagt tggaaaacat
     3121 cgcgctgctc atgttcgtgc tggcgctgtt cttcatcccg atcctgctcc tgcggcatca
     3181 tctctacgaa tggatgaaca tcccgcgcgg tcaggaagag aacctcgact ccaaacgcgc
     3241 ttacctgaac tggaccttct ttgtcgtccg cgccgtcatt ttcctcggct tcttcatcgt
     3301 tgcgacgttt ttgctgcggc gcttttccgt acgccaggac aaggacggca atccgcgttt
     3361 taccatcctg atgcgcaaag tcgcgttcgc cagcctcccg atgtttgcgc tttgtctcac
     3421 cttcggtgcg ttcgattggc tgatgagcct gaactaccgc tggttctcca ccatgttcgg
     3481 cgtttacatt ttcgccggca cggccggcgc ctcgatgtcg ttgctcgtgc tcgtcatcac
     3541 ggcgttgcgc aacgccggtt acctcaagga cgtggtcacg atggagcatt accacatcat
     3601 ggggaaatgg atgttcgcct tctgcacttt ctgggcctac atcggctttg gccaatacat
     3661 gctcatctgg tatgcgaaca tgccggagga gacccaatac ttcctcgtcc ggacgaccca
     3721 gtcgtggtgg gggttgagca tgctgcttgt gttcggccgt ttctttgggc cgttcgcgat
     3781 tttgcttttg cgttcaatca aaaaacatcc gcgccaactc tgttatgtcg ccggctggat
     3841 cctactcatg cagatgctcg atatgtatct ggtcgtgttg cccgccctgc acggcaccgg
     3901 cgtccacctt agcctgtggg actttgttac ggtgatcgcg ttcggcgcca cactggcgtt
     3961 cgtttacctg cggattgtcg gcaaaacttc gctcttccca gtgcgcgatc cgcggctggt
     4021 cgaatcgttg cgcctggtaa attgatatgg ccgacgccga atccatccgc caagctgtgc
     4081 gcgcccgcgc gccattttca acgtggctcg gtgtgatcgc gctcttcttt ctattcggcg
     4141 tcatcgtgct cgcggtcgtc gggccggcgc cgcgcgggga cacctatgaa gagatgcgcg
     4201 ctagaaagcg gatcgacaac ttgaaagcgg cgcgtgaggc cgatgcgaag gaactcggga
     4261 cgtacggctg gatcgacaag aacaaaggca ctgtgcgcgt gccgatcgat cgcgcgatgc
     4321 aactcgcagt cgcggaattg gccaaacaaa agccagcgcc cgcttacgcg attgcgccgc
     4381 ccgctccgca agcatcacaa gcgccagccg gcgctgccac tcccgcggca cctgctttac
     4441 ctacgccgca gccgagcgct acgcccaaac caatttcagt tgcaggcccc gaatcggaaa
     4501 accgcgcgca accggcggcc gcggcgaatc cgccgggggc acaacccgga acgcaacccg
     4561 gcgcttcggc gagtccggcc gcttcacccg cgtcgtcgac cgcacaacca gccgttcctc
     4621 catcgccgcg acctgcgccg tcaacgccgc ccggaagtcc tttgccggtg cgcgggaaaa
     4681 caccgccgcc aggccaatga cgtcgtcagc agataagatg tcgatcttac acgcaaaaat
     4741 atggacgcca ctgagatccc gctgaaagcg cccgaacttc cgacgcacgc tcccgtcact
     4801 gagacggagc aatttgaacg cgcgttgatc gacgcatcga cgcgcgtccc ggttttgatg
     4861 ttttacgcgt cggcgatttt ctggctgctc gccggcacaa ttctcggtgg gctcgtctcc
     4921 tggaaattgc attcacccga tttgctttcg ggtgtgagtt tcctgacctg gggccggctg
     4981 cggccggtgc atatgaacat catggtctac ggctgggctt cgatggcggc gatgggcacg
     5041 ggcgtttgga tcatggcccg actctgtcgg acggtgttgc ggcgtccgct cgtgattgtt
     5101 gccggtgcag gtttttggaa tctcggggtg ctgatgggcg tttgcggaat tttgctcggg
     5161 gacagcaccg gttatcaatg gctcgagttt ccgccttacg cgtcgatcgt tctcttcgcc
     5221 gcctacacgc tgatgctctc gtgggccgtg ctcatgttcc ggttccggcg cggcgaacaa
     5281 atttacatta cccaatggta tttgctcggc gcgttcctct ggttcccgtg gctctacgcc
     5341 gccgcgcaat tgatgctctt cgtcgtaccg gtgcagggcg tgatgcaggc ggccgtcacc
     5401 tggtggtacg cgaacaacgt gatgttcctc tggttaggcg cgatcgcgct cggcaccgcc
     5461 tactacatga taccgaaagt aatcgggcgc ccggtttaca gttatcacct cgccacaatc
     5521 ggtttctgga cttacgcgct cttttccagt tggaccggca tgcaacggct ggtcgacggt
     5581 cctttcccgg cgtggatgat caccgcgagc atcgccgcga ccattctcac gctcattccg
     5641 gtcgcgacgg ttgggctcaa ccaccacatg acgatgctcg gatatttccc gctgctgcgt
     5701 tatagcccaa cgctccgctt cacggtcttc ggcgcgatgt cttacacggt tttcagcggc
     5761 gtcggaattc tgatttcgct ccgttccatc gcgcgctacg tcaatttcag cgaggcgagc
     5821 atcggctact cgcacctcgg cctttacgcg tttgtcacga tgatcctgtt cggctcgatg
     5881 tattacattg tgccgcggct ggtcggccgg gaatggcgtt acgctaccct gatcaaattg
     5941 catttctgga gctcggccta tggcatcggg ctgatgacgt taatgctgct cgccggcggt
     6001 ttcttacaag gcgctaacat cgacaatcca tcactcgagt tcaccgagag cgtgcaaaca
     6061 attctgccgt acctgcgtgg gcgcagtctt tccggaatac tgcttaccgt ttcgcatttt
     6121 gttttcgcgt atcacttcgg tctcatgctc ttcggcctcg gccgaacatc gagcgtgccg
     6181 acattcttaa atcctgtgga agcggaaggt tccgaggaac acctatgaag ggactcgctt
     6241 cgctcatcct cggcattttt gggacgttta gcttctcttg gctcggtttg atcctggttc
     6301 cgaattacca gatcggacat ctgaatccgc aaatggacga ggaagggacc gatgtttatc
     6361 ccacgccaaa gtccggaatg gccgagcgcg gtcgtcgcgt ttacgtcgcg aacggctgct
     6421 attactgcca cagcgagcag gtgcgtcccg attacgcggc gggcgatatc gaccgcaaat
     6481 ggggcgaccg ccggagtgca ccgcgcgact acatttttga tcggccggtg ttgctcggca
     6541 aagaacgcat gggccctgat ctttcgaatg tcggcaaacg cgctcccgcc gaaggcgagg
     6601 cgccaaacgc ggcgaatgcg gcaatcactg ctgcaccagg cggcggttcg ccggcgccac
     6661 ctgctccacc ggcgggcgga gcgccagcgc agccgaagac gcctcccgcc ggatcaccgg
     6721 ctcaatcacc cgcagctgcg ccgcaaacac cgccgaatgc cccggcgcga cccgctgcgt
     6781 cgccgcctgc acaaaatccg ccgccggccg ccgctggatc acccgcgcaa tcaccggccg
     6841 caacgagcgc gccggccgct gcaaacgcgc cgcctcctcc gccggccgct gcgctatcac
     6901 cgtcagtgaa ccctccaacc ggtgagccgc tgccgtattc cgcggcgtgg catcatcgac
     6961 atctttatgc gccgcgcagc gtcactctcg actccaatat gccggccttt cgcttcctct
     7021 acgaaaagcg cccgagcggc agcgagcctg cagcgaatgc cgttcagctc accgcgaccg
     7081 acgcgccgcc agtcggctgg gaagtcgttc cgacatacga cgccgaatgt ctcgtcgcgt
     7141 acttgatgtc gctcgatcaa tcgcatccgt tgaaagaagt gaaatctgca gg
//
LOCUS       DASTDG010000165         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_222843, whole genome shotgun sequence.
ACCESSION   DASTDG010000165 DASTDG010000000
VERSION     DASTDG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222843"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2424)
                     /locus_tag="VFO30_05445"
     CDS             complement(<1..2424)
                     /locus_tag="VFO30_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9418766.1"
                     /translation="MIRDFKFAFRQLFKAPAFTISAVTVLALGIGVNTAVFTLVNRLF
                     FAPPAYAKPHEMVQLFSQDKKDPKKFRGFSYPTYLDIREQNTVFSDVMAFNLAFIGLG
                     QKGDTRRAFSAIVTSNYFSVLGVPLARGRAFLPEEETPGHNTPVAIVSYNYWQKHDLD
                     PNLLGSQLLINGRSFTIVGVTPKGFVGTMQILSPEVWLPMSVYDQVANDFRSENRTTI
                     DDRKGTQLRIMGRLKAGMTAAASKPALEGLAANLEKAYPVEQKNQTLIAAPVSRNSVS
                     NNPAAESGIKVIAPLLLGMAVVVLLVACLNLANMLLARGTARRKEIAIRLALGGSRWR
                     IVRQLLTEGFALAVLGGVGGLILGLWSSDLLVGSIRKLMPLDIVWLGGPNPAILAATF
                     GFCLLGTLMFALGPAVKISRSAVVADLKEHAGEDVVRRRWKFLPRHPLVVVQIAFSLA
                     LVTAAALFIRGAGKAASVDTGLRPGASYVLEVDASLAGYEPKRAQDVYRNLNERLAGL
                     PGVEHASISSIVPFGMFELSRKVQRAGLRLAPDAKPATAAEGLALEVAWNSVGADYFS
                     TVGLPVFRGRAFTEAEATQPGPKVAIIDDVLAKKLWPDGDALGQRIQYAGENAPSAES
                     GGAHVGRSGDVSEEEKQGETLEVVGIVPATRHALFEREPIGGIYLPFARGFQNDVSFF
                     VRFRSLASGNEAATADLLRRTVRDVDPSIPILSLRTFAQHLDSNLDLWLVRAGAALFS
                     IFGALALGLAVVGLYGVKAYSVARRTREIGIRMALGAQAGAVLRMIMREGSIMLFSGV
                     TIGLLLAIAT"
     gene            complement(2427..>2766)
                     /locus_tag="VFO30_05450"
     CDS             complement(2427..>2766)
                     /locus_tag="VFO30_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9418767.1"
                     /translation="AIGIYGVMAYSVTQRTQEIGIRMALGAQRSDVLGLVVRQGMMLA
                     AIGVVAGLAGAFGLTRVMASLLFNVRPDDPTTYLAISFLLTAVAFLACYLPARRAAKL
                     DPMIALGHGA"
BASE COUNT          633 a          847 c          763 g          523 t
ORIGIN      
        1 cgtcgcgatc gcaaggagca ggccaatggt cactccactg aacagcatga tggaaccttc
       61 acgcatgatc attcgcaaca ccgctccggc ctgagcgccg agcgccatcc ggattccgat
      121 ctcgcgcgtg cgtcgagcca ctgagtacgc tttgactccg taaagaccaa caacggcgag
      181 accgagcgca agcgccccaa agatggagaa caacgccgcg cccgcgcgaa cgagccagag
      241 gtccaggttc gaatcgagat gttgcgcgaa cgtccgcagc gaaaggatcg ggattgatgg
      301 atcgacatcg cgcacggtgc gtcgtagcaa atcggctgtg gcggcttcgt ttccagacgc
      361 aagcgaacga aaccgaacaa aaaaggaaac gtcattttgg aatccgcgcg cgaacggcaa
      421 gtaaattccg ccaattggct ccctttcaaa gagcgcatgg cgcgttgccg gcacgatgcc
      481 gactacttcg agcgtctctc cctgcttctc ctcttcactc acatccccgc tccgtccgac
      541 gtgggcacct ccgctctcgg ctgacggcgc attttcaccc gcatactgaa tgcgttgtcc
      601 aagcgcatca ccatccggcc agagcttctt tgccagaacg tcgtcgataa tcgcgacttt
      661 cggaccgggc tgggtcgcct ccgcttcggt gaacgcgcga ccacgaaaaa ctggcaatcc
      721 aacggttgaa aaataatctg cgcccacgct gttccaggcc acctctaagg caaggccttc
      781 ggcggccgtc gccggcttag cgtcgggagc gagacggagg ccggcgcgct ggacttttct
      841 gcttaactca aacatcccga acgggactat tgaagagata ctcgcgtgct ccacgcctgg
      901 cagccccgcc agtctctcgt tcaagttgcg ataaacgtcc tgcgcgcgtt tcggttcata
      961 acccgccagg ctcgcatcga cttccagaac atagcttgcg ccgggcctta atccggtatc
     1021 gaccgacgcg gccttgcctg caccacgaat gaacaaagcc gcagcggtaa cgagcgcgag
     1081 tgagaaagcg atttgcacga cgacaagtgg gtggcgcggt aagaatttcc accgccggcg
     1141 caccacgtct tcccctgcgt gttctttcag atcggcgacc accgcgctac gcgaaatttt
     1201 cacggccggc ccaagcgcaa acatgagtgt gccgagcagg caaaatccga acgttgccgc
     1261 caaaattgcc gggttcggtc cgcccagcca tacgatgtct agcggcatca gtttgcgaat
     1321 tgaaccaacc agcaaatcgg acgaccagag tccaagaatc agtccaccca cgccgccgag
     1381 caccgccagc gcaaagcctt ccgtaagaag ctggcgaacg atgcgccacc ggctgccacc
     1441 gagcgcgaga cggattgcga tttctttgcg gcgcgcagta ccgcgtgcga gcagcatgtt
     1501 agcgaggtta aggcatgcca ccagcagcac cactaccgcc attccgagca gtaatggcgc
     1561 gatgactttt atcccgctct cggccgccgg gttgttactt accgaattac gcgacacggg
     1621 cgccgctata agcgtttgat tcttctgttc gactggatag gccttttcca aattggccgc
     1681 gagcccttcg agcgcaggct tggatgcggc cgcagtcatc ccggctttaa gccggcccat
     1741 gatgcgcagc tgcgttcctt tgcgatcgtc aatggtagtc cggttttccg acctaaaatc
     1801 gttagcgacc tgatcataaa cactcatcgg caaccaaact tccggcgaca gaatttgcat
     1861 cgtgccgacg aaaccctttg gcgtaactcc cacgatggtg aacgagcgac cgttgatcag
     1921 taattgcgag ccgagcaggt ttggatcgag gtcgtgcttc tgccagtaat tgtaactgac
     1981 gatcgcgacc ggcgtgttgt ggccaggcgt ttcctcttcc ggcaaaaatg cacggcctcg
     2041 cgccaacgga acgccgagca ccgagaaata gttcgaggtg acgatggcac tgaatgcgcg
     2101 ccgcgtatcg cctttctggc caaggccgat aaacgcgaga ttgaaggcca ttacgtcgct
     2161 gaacaccgtg ttctgctcgc ggatgtcgag ataagtagga taagaaaaac cgcgaaattt
     2221 cttcggatcc ttcttgtcct gagaaaagag ctgcaccatt tcatgcggct tggcataagc
     2281 cggcggggca aagaaaagcc tgtttaccag ggtgaatacg gcggtattca cgccaatgcc
     2341 gagcgcgagc acggttaccg ctgaaatcgt aaacgccggc gccttgaaca gctgccggaa
     2401 cgcaaattta aaatcacgaa tcatgattaa gcgccatggc ctagcgcgat cattggatcg
     2461 agtttcgcgg cgcgacgcgc gggcagataa caggcgagaa atgcgacggc cgtgagcaaa
     2521 aacgaaatcg cgagatacgt cgtcggatca tccgggcgca cattgaaaag caaacttgcc
     2581 atgacgcgcg tcagtccgaa ggcgccggcc agtccggcga caaccccaat cgcggccagc
     2641 atcattccct gcctaacgac tagtccgagc acatcacttc tctgcgcacc aagcgccatg
     2701 cgaatgccga tttcctgggt gcgttgcgtg accgagtagg ccatgacgcc gtaaatgccg
     2761 atcgcc
//
LOCUS       DASTDG010000166         6650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_224943, whole genome shotgun sequence.
ACCESSION   DASTDG010000166 DASTDG010000000
VERSION     DASTDG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224943"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(763..1758)
                     /locus_tag="VFO30_05455"
     CDS             complement(763..1758)
                     /locus_tag="VFO30_05455"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9418768.1"
                     /translation="MKKYHIAIVGATGAVGSELLGVLKRRNFPVASLRPISSVQSAGK
                     SVAFGGALHGVEELNERSFANIDLAFFSAGGEVSRQWVPIARENSAVVIDNSSVFRME
                     PDVPLVIPEINGEDVPRHRGLIANPNCTTAVALMALYPLHRVFQVRRIFAASYQAVSG
                     SGTRAIEELKVQTEAALQDRQLSAKVYPHPIAFNVLPRIDAFLENGYTKEEMKMQNEG
                     RKIMHLPEFRASVTCVRVPVVRAHSVAVSAEFEKPVSVDQAREVLAKAPGLELVDEPQ
                     NERYPMPLNVAGKDNCQVGRVRIDCAFENGLSFWVSGDQLLKGAALNAVQIAELL"
     gene            1791..2252
                     /locus_tag="VFO30_05460"
     CDS             1791..2252
                     /locus_tag="VFO30_05460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9418769.1"
                     /translation="MQRPKTKIDISVRDQTLTVTRGDETLRTYPISTSRFGLGTEQNS
                     FKTPTGLFRIADKIGDGMALDTIFVGRVPLGPQDPLPTTDDFVMTRILWLEGMEERNA
                     NTRDRYIYIHGTKHEDKIGTPASHGCVRMCNADVAELFDLVEEGTPVVIRE"
     gene            2635..4134
                     /gene="hutH"
                     /locus_tag="VFO30_05465"
     CDS             2635..4134
                     /gene="hutH"
                     /locus_tag="VFO30_05465"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9418770.1"
                     /translation="MQLNGQPLSLSDLAGVAFGDERVEISPSALPRIHACREVIEKIV
                     ARDAAVYGVNTGFGKLADVRIQPNEFRELQLNLVRSHACGIGNPLSEPEVRAMMLLRA
                     NVLTLGFSGIRAEIIEMLCQMLNRRVCPVVPEKGSVGASGDLAPLAHLALALIGEGDA
                     LLTGERMPSSEALRRAGLKPAELEAKEGLALLNGTQAMHAVGGLSIFRAQRLTTVVDV
                     AGAMSLEALLGTPAAFDQRLQDARPHAGQKAVAKHLLSILQGSEIRESHRENDPRVQD
                     AYSLRCMPQVHGAVRDALAHGESILQVESASATDNPLVFTDTGEVISGGNFHGAPLAL
                     AFDYAGIAMTDLMSISERRIDRLVNPDLNEGLPPFLARHAGSQCGFMIAHVAAVSLLN
                     EAKVLAHPASIDNVPTSGGKEDHVSMGMTSALKLRSIVDLAENLVAIELLAAAEALEH
                     RHPLKAGVGVERAFAIIRKIAPPLTQDRALSADIARMAESIRAGNFDDI"
     gene            4131..5798
                     /gene="hutU"
                     /locus_tag="VFO30_05470"
     CDS             4131..5798
                     /gene="hutU"
                     /locus_tag="VFO30_05470"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9418771.1"
                     /translation="MSGKRIIRAPRGGDLTCKGWHQEAAMRMLMNNLDPDVAEDPDRL
                     VVYGGTGRAARSWEAFDAIVRSLQNLQNDESLLVQSGKPIGKFRTHDQAPRVLIANSN
                     LVGQWNNYAEFNRLERLGLTMYGQMTAGSWIYIGSQGIVQGTFETFAAAGRKNFGGSL
                     DGKFVLTGGLGGMGGAQPLAATMNGAVFLGVEVDPARIDKRLKSGYCDKIAWSLDEAL
                     KIVEQAQKDRQSLSVGLVGNCADVLPEFVRRGIVPDVLTDQTSAHDALNGYVPNEMTL
                     EDALALRRNNPEEYIERSMRSMAVHVEAMLALQRKGAVTFDYGNNIRAQAQKAGVKNA
                     FDIAGFVPEYIRPLFCEGKGPFRWVALSGEPDDIARTDKLALELFPQNQTLARWMKLA
                     KERIHYQGLPARICWLGYGDRAEFGVAINELVRRGEIKAPIVIGRDHLDAGSVASPNR
                     ETEGMLDGSDAIADWPLLNALVNTAAGASWVSIHNGGGVGIGFSQHAGMVVVADGTEN
                     SKRRLERVLTSDPGTGVLRHVDAGYSRAIEFAATDKIDIPMPPRPRD"
     gene            5802..>6650
                     /gene="ftcD"
                     /locus_tag="VFO30_05475"
     CDS             5802..>6650
                     /gene="ftcD"
                     /locus_tag="VFO30_05475"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET9418772.1"
                     /translation="MQKLIECVPNFSEGRDQNIIRQITAAIEAVDGVSLLDVDPGAST
                     NRTVVTFVGMPDSAVEAAFRAIQRAAELIDMRKHKGAHPRMGATDVCPFIPVSNVSWE
                     EAINCARKLGQRVGEELNIPVYLYERAARDKSRANLAVVRAGEYEGFFDKIKQPSWKP
                     DFGPAVFNAKAGATVIGARDFLIAYNVNLNTRSVRRANAVAFDVREQGRIKTEDGTPS
                     GKPVLDANGEPVRVPGMLKQVKAIGWFVEEYGIAQVSMNLTNIEETPLHVAFDACCEA
                     AMKRGLR"
BASE COUNT         1403 a         1974 c         1973 g         1300 t
ORIGIN      
        1 tgcggtgaca ggcaccgctg cagcccaagc ttccccagcc gctcccgcaa gtcccgcggc
       61 aacagcgctt ggcgcgacga gcgctacttc tcccgtcgag cctggcaggg ccggagcagc
      121 agcggctgag cgcggacaag ctgctgcaac tacatcgcca gctgtgactt cgccatcacc
      181 tgccgtgcgt ggtaagcacc atcgcgggcg accgggcgaa caaattggtc ggagcccagc
      241 ggcgggtgct gcggcaacag cagccccagc tgcaacgacg tcgccgcacg aaggtcgagg
      301 caaacacaaa ggcgaggttg agcgcgccac gcccgctgct ccgcagggcc aaccgggtgc
      361 actccctcgc gaaacgccac gtgggcgacc gcaaggtccg ccaccgagcg ccacaccgtt
      421 tacgcagacc ggtgaaaatc tacaggagcg cggcccagca cagacagctc cagagcgcgg
      481 tgccgcgcca gcggcaatcc gaccattgcc ggcagaacgc gggccagctg ctgaacgccc
      541 tgaacgtgga tcggctgctc aacgcgagtc ggctgctgaa cggcgggagc ctggaccggc
      601 tggggcgcct gggagaaccg aaggcggaaa aaagaagcca ggagcatctc cttctccgtc
      661 accctaacaa gtcacgggtg aattgatcgt cagccccgga gaaatccggg gctgatttgt
      721 tttagatcgg caattaatca gaacggcgac agcgccccat tgctacagca gttctgcaat
      781 ctggactgcg ttgagcgccg cgcctttcag caactggtct ccactcaccc aaaacgaaag
      841 accgttttca aacgcgcagt cgattcgcac gcgcccaacc tgacaattgt cttttccggc
      901 gacgttcaac ggcatggggt agcgctcgtt ttgcggttca tcgacaagtt ccagccccgg
      961 cgctttcgcc aagacctcgc gtgcttgatc gaccgatact ggtttttcaa attctgcgct
     1021 gaccgcgacc gaatgggccc gaactaccgg tacgcgcacg cacgtaaccg aggcgcggaa
     1081 ttccggcaga tgcatgattt tgcggccttc attttgcatt ttcatttcct ctttcgtgta
     1141 gccgttctcg agaaacgcat cgatgcgcgg cagcacgttg aacgcgatcg gatgcggata
     1201 gaccttggcc gataattgtc gatcttgcag cgccgcttcc gtctgcacct tgagctcttc
     1261 gatcgcgcgc gtgccgctgc cggacaccgc ctgatagctc gctgcaaaaa tccggcggac
     1321 ttggaaaacg cggtgtagtg gatacagcgc catcaacgcc actgccgttg tgcaattcgg
     1381 gttcgcaatg agcccgcgat gtcggggcac atcctcgccg ttgatctccg ggatgaccag
     1441 aggcacgtca ggttccattc ggaagacgga tgaattgtcg atgaccactg cgctgttctc
     1501 ccgcgcaatt ggaacccact gccgtgacac ttcaccgcca gcactgaaga acgcgaggtc
     1561 gatattggcg aaggagcgct cgttcagttc ttcgacgcca tgcaacgcac cgccgaatgc
     1621 aaccgattta cccgcagact ggaccgagct aatcggccgc aaactggcga ctggaaaatt
     1681 gcgtcgtttc aggacgccca acagctcgct gcccaccgcg ccggtcgctc cgacaatcgc
     1741 gatgtggtat tttttcacga gcaagcggac attaccgcgg ccggtttgtc atgcaacgtc
     1801 ccaagaccaa gatcgacatc tccgttcgtg accaaacact taccgtgacg cgcggcgatg
     1861 aaactttgcg gacctacccg atttcaactt cgcgcttcgg attaggaact gaacaaaaca
     1921 gtttcaaaac gccaacggga ctgttccgca tcgctgacaa aatcggcgac ggcatggctc
     1981 tcgacactat ttttgtcggc cgcgtgccgt tgggtcctca ggacccgctt cccacgactg
     2041 atgattttgt catgacgcgg attctctggc ttgaagggat ggaagaacgt aacgcgaaca
     2101 cgcgcgaccg ttacatttac atccacggca caaaacacga ggacaagatt ggaacgcctg
     2161 ccagccatgg ctgcgtccgg atgtgcaacg ccgatgtggc cgaattgttc gacttagtcg
     2221 aggaaggtac gccggtagtc attcgcgagt aacgcgtcct tttgacacat cctgctgcgc
     2281 aggcgcgcac gtttctaaag cgtgagaaaa gcgttgctta cgcctcttcc ctgcctaata
     2341 agcagccagc aacctgctcg gaaaggaggc gatatagatt gagattcctg aaagcgatca
     2401 tgctcctggc cgttttggcc agcacgttga gtctgggcgc ttgcgcccac aaacaagaga
     2461 ccatgtccac tgggacaacg gccagcacgc gcgggtattc caaatagctc cgcggcgtgc
     2521 ccatctgcgg atggattcaa taaagggcca ggtcgctgtc ggcctggcct tttcgttctt
     2581 tgtgctcaac gcttcaacgc tttcaccctt caacggttct cgttacagtt ggccatgcaa
     2641 ctgaatggcc agccgttgtc cctcagtgac cttgctggcg tcgcgtttgg tgatgagcgc
     2701 gtggaaattt caccctccgc gctcccgcga attcatgcgt gtcgcgaagt tattgaaaag
     2761 atcgtcgcgc gcgatgccgc ggtctacgga gtcaacaccg gattcggaaa gctggccgac
     2821 gtgcgcatcc agcctaacga gttccgcgag ctgcaattaa acctcgtccg cagccatgcc
     2881 tgcggcattg gcaacccgct ttcggaaccg gaagtgcgcg cgatgatgtt gctccgcgct
     2941 aacgtgctca cgctcggatt cagcggcatc cgagccgaga tcatcgagat gctgtgccaa
     3001 atgctcaatc gtcgcgtctg tcccgtggtt cccgagaagg gttctgtcgg tgcgagtggt
     3061 gatctggcgc cgctcgcgca tctggccttg gccttgatcg gagaaggcga cgcgttattg
     3121 acaggtgagc gaatgccaag ctccgaagcg ttgcgccgcg cgggcttaaa accggcagaa
     3181 ctcgaagcaa aagaaggcct ggccttgctc aacggcacgc aagcaatgca cgcggtgggt
     3241 ggattgtcga tctttcgcgc acaacggctt actaccgtcg tcgatgtcgc cggcgccatg
     3301 agcttggaag cactgctcgg cacccctgcc gcgttcgatc aacgtttgca ggatgcgcgg
     3361 ccgcacgctg gacagaaagc cgtggccaaa catcttttgt caatcttgca aggcagcgaa
     3421 atccgtgaat cgcatcgcga gaacgatcca cgtgtgcagg acgcctacag tttgcgatgc
     3481 atgccgcagg tgcacggcgc cgtacgcgac gcgctcgcgc acggcgagag catcctccaa
     3541 gtcgaatccg cgtccgcgac cgataatccg cttgtcttca cggataccgg cgaagtcatt
     3601 tctggcggca attttcatgg cgcgccgctt gcgctcgcat ttgattatgc gggaatagcg
     3661 atgactgatt tgatgagcat cagcgaacgg cgcatcgatc gactcgtgaa tccggatttg
     3721 aacgaaggtt tgccgccctt cctcgcgcgc cacgccggct cgcaatgcgg attcatgatc
     3781 gcgcacgtcg ccgctgtctc tttgctaaat gaggcgaagg tcctggcgca cccggcgagc
     3841 atcgataacg tgccgacctc cggtggaaaa gaggaccatg tctcgatggg aatgacctcc
     3901 gcgctcaaac tgcgatcgat tgtcgatctt gcggaaaatc tggtcgccat cgaattgctc
     3961 gccgcggccg aagccttgga acatcgacat ccactaaaag ccggagtcgg ggtcgaacgc
     4021 gcttttgcca tcatccgcaa aatcgcgccg ccgctgacgc aggaccgcgc tctctccgcc
     4081 gacatagcgc gcatggcgga aagcattcgc gcaggtaact tcgacgacat atgagcggga
     4141 aacgaattat tcgcgcgcca cgcggcggcg atctcacctg caaaggttgg caccaggagg
     4201 ccgcgatgcg gatgctgatg aacaatttgg atccggacgt cgccgaagat ccggatcgac
     4261 tcgttgtcta cggcggaacc ggtcgtgctg cgcgcagctg ggaagcattc gatgcgatcg
     4321 ttcgctcgct ccaaaatttg cagaacgacg agtcattgct cgtgcaatcg ggtaaaccga
     4381 ttggcaaatt ccgcacccac gaccaagcgc cgcgcgtttt gatcgccaac tccaatttgg
     4441 tcggacagtg gaataactac gccgagttca accgcctcga gcgacttggc ctgacaatgt
     4501 atggccagat gacggccggc tcgtggattt acatcggcag ccaggggatc gttcagggca
     4561 cgtttgaaac ttttgccgct gcgggtcgca agaatttcgg tggctcgctc gatggaaaat
     4621 tcgtgctcac cggcggtctc ggcgggatgg gcggtgcgca accgctcgcg gcgacgatga
     4681 atggcgccgt gttcctcggc gtcgaagtcg atccggcgcg tatcgacaag cgattgaaga
     4741 gcggctactg cgataagatc gcgtggtcgc tcgacgaagc gttgaagatt gtcgagcaag
     4801 cgcaaaaaga tcgacaatcg ctctccgtcg gcctcgtcgg gaattgtgcc gacgttctcc
     4861 ccgaattcgt taggcgggga attgttcccg acgtgttgac cgaccagacc agcgcgcatg
     4921 atgcgctaaa cggttatgtc cccaacgaaa tgactctgga ggacgcgctc gcgttgcgcc
     4981 gcaacaatcc tgaggagtac atcgagcgct ccatgcgatc catggccgtg cacgtcgaag
     5041 cgatgctcgc gttgcagaga aaaggcgcag tcacattcga ttacggtaac aacattcgcg
     5101 cgcaggcgca gaaagccggc gtgaaaaacg cgttcgacat cgccgggttc gttcccgagt
     5161 acatccggcc gctcttttgc gaaggcaaag gtccattccg ctgggtcgcg ttgagcggcg
     5221 agcccgacga catcgcgcga accgacaaac ttgcgctcga attattcccg caaaaccaga
     5281 cgctggcgcg gtggatgaaa ctcgcgaagg agcgcatcca ttatcagggc ttgcccgcgc
     5341 ggatttgctg gcttggctac ggcgaccgtg ccgagtttgg cgttgcgatt aacgaacttg
     5401 ttaggcgggg cgaaatcaaa gcgcccattg ttatcggacg cgatcatctc gacgccggtt
     5461 cggtcgcgtc gccgaaccgc gaaaccgaag gaatgctcga cggcagcgat gcgatcgcgg
     5521 attggccgtt gctcaacgcg ctcgttaaca ccgccgccgg cgcatcatgg gtttccattc
     5581 acaacggcgg cggggttggc atcggtttct cgcagcatgc cggcatggtt gtcgtcgccg
     5641 acggcaccga aaattccaag cgccgtctcg aacgcgtgct cacgagcgat ccaggcacgg
     5701 gcgtcctccg tcacgtcgat gccggctact cgcgtgcaat cgaattcgcc gccacggaca
     5761 agatcgacat cccgatgcca ccgcggccgc gcgattgatc aatgcagaag ctgatcgagt
     5821 gcgtgccgaa tttttcggag gggcgcgacc aaaacattat tcggcaaatc acagcggcga
     5881 ttgaagcggt tgacggcgtc tcattgctcg atgtcgatcc cggcgcgagc acgaatcgaa
     5941 cggtggtgac gttcgtcggt atgccggact cggccgttga agcggcattt cgcgcaattc
     6001 aaagagctgc cgagttgatc gacatgcgca agcacaaagg tgcgcatccg cgcatgggtg
     6061 cgacagatgt ttgcccgttc attccggtga gcaacgtcag ttgggaagag gcaatcaatt
     6121 gcgcgagaaa gttggggcag cgtgtcggcg aggaattgaa cattccggtt tatctttacg
     6181 agcgcgcagc ccgcgacaaa tcgcgcgcga atctcgccgt cgttcgtgcc ggcgaatacg
     6241 agggcttttt cgacaagatc aaacagccca gctggaagcc agatttcggt cccgcggtgt
     6301 tcaacgccaa agctggcgcg acggtaatcg gcgcgcgcga tttcctgatc gcttacaacg
     6361 tgaacctgaa cacaagatcg gtgcgtcgcg ccaatgcggt cgcgtttgat gttcgtgagc
     6421 aaggccggat caaaacggaa gacggcaccc catctggaaa accggtgctg gatgcaaacg
     6481 gcgagccggt gcgcgttccg ggaatgttaa agcaggtgaa agcgatcggc tggtttgtgg
     6541 aagagtacgg gatcgcgcag gtgtcgatga acctgaccaa tatcgaagag acgccgctgc
     6601 acgttgcctt cgatgcctgt tgcgaggcag cgatgaagcg cggtctgcgt
//
LOCUS       DASTDG010000167         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1669277, whole genome shotgun sequence.
ACCESSION   DASTDG010000167 DASTDG010000000
VERSION     DASTDG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669277"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFO30_05480"
     CDS             complement(<1..917)
                     /locus_tag="VFO30_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET9418773.1"
                     /translation="MEKTARDIAHQLREQGHIAYFAGGCVRDIVRRETPKDFDIATDA
                     TPAIVQQIFPRTYAVGAHFGVIVVVENGFQFEVATFRSDDAYVDGRRPTAVHFSSPEE
                     DAKRRDFTINGMFYDPVAEKVIDFVGGRADLEAKVLRAIGEPAQRFQEDRLRMLRAVR
                     FATVLDYKIDNQTWDAIVANAAVITGISAERIRDELVRIFLSPNRVRGWDLLDESGLM
                     RAILPEVVAMKGCAQPEQFHPEGDVFAHTRLMLKLLPEKVSVPLVFAVLLHDVAKPIT
                     ATVDQTGRIRFNEHDRIGAAMTEQIMERLR"
     gene            complement(977..1456)
                     /gene="lspA"
                     /locus_tag="VFO30_05485"
     CDS             complement(977..1456)
                     /gene="lspA"
                     /locus_tag="VFO30_05485"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9418774.1"
                     /translation="MKFIFFLSLPLYALDQVTKFWVRGALGTEEPRIVVPGFFSLVNI
                     TNTGAAFGSFKNNNEFFVALSCLALLFVVVLLLRRRSRDFWRDLSLGLLLAGVLGNLT
                     DRLLYGHVIDFLLFDLHVPFAHPWPAFNIADSCITLAVVSFIINSFRQTKSTSETAT"
     gene            complement(1561..>3688)
                     /locus_tag="VFO30_05490"
     CDS             complement(1561..>3688)
                     /locus_tag="VFO30_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9418775.1"
                     /translation="YVKFPIATGEWKDKASIAIWTTTPWTLPANMAIAVSPKDLYVVQ
                     EFMRDGAFETLLLADKLVPQFCAHTKFDPVGEPKASFTGDKIDKIRARHPFLDREAVV
                     LLADFVKMDSGTGAVHIAPGHGADDYWLGYNNGLAILSPVDDHGRFTAEASLPKLTGK
                     YVFDANHDIINLLRERGNLLGQQTFHHSYPYCWRSKTPIIFRAVEQFFIRIDELRRQA
                     LAAIHNEVRWIPGWGENRIAGTVEARPDWVISRQRSWGVPLPVFYSKTGEAILDAKII
                     RKLADLVAERGSNIWFELDDVDLAKQLGLPEGTTKRNDTIDVWIDSGVSHKAVCAIHP
                     ELRDPADMYLEATDQHRGWFQSSLMTSIALNGRAPYKACVTHGFVVDLDGKKISKSGT
                     YDKPTDAGHFVGRHGADLVRLWASSIDYTDDVPFSEEMFTRLGDTYRRIRNTLRILLG
                     NLFDFPGAGVAEAGPRSTTAATDLTLIDRWILERLDQVVADCRAAYQAFEFYKVYHAI
                     NQFCAVDLSSLYVDITKDRMYCDAPDSPRRRATQAAMHEIFNALCQLLAPILAFTAEE
                     AWRHSGATALVHLEEFPTPRNRGRQATEAIAELLRLRGIIGQALEQARQQKLIGNSLE
                     AAVVLSSDSDVTRKIDKNELEEFFIVSDLTVEQASEASVSITRTGFQKCVRCWRHRST
                     VGASKAHPELCDRCEAVVALLANRAR"
BASE COUNT          778 a         1070 c         1082 g          758 t
ORIGIN      
        1 cgcaaccgtt ccatgatctg ttcggtcatg gccgcgccga tccggtcgtg ttcattgaaa
       61 cggatccggc cggtttgatc gaccgtcgcg gtgatgggct tggccacatc gtgcagcaaa
      121 actgcgaaca cgagtgggac cgagactttc tccggcagca atttgagcat tagccgcgtg
      181 tgcgcgaaga catcgccttc cggatgaaat tgctccggtt gcgcgcagcc tttcatcgcc
      241 accacttctg gcagaatcgc gcgcattaag ccgctttcat caagcaaatc ccagccgcga
      301 acacgattgg gggacaggaa aatccgcacc aactcgtcgc ggatccgttc ggcgctgatc
      361 ccagtaatga ccgccgcgtt tgccacaatt gcgtcccacg tctgattgtc gatcttgtag
      421 tcgagcaccg tcgcgaaccg taccgcgcgc aacatccgta accggtcttc ctggaatcgt
      481 tgcgccggtt cgccgatcgc gcggagtact ttcgcctcca gatccgcgcg gccaccgacg
      541 aaatcgatca ccttctccgc gactggatcg taaaacatcc cgttgatcgt gaaatcgcgc
      601 cgtttcgcgt cttcttccgg cgaggaaaaa tgcaccgccg ttggccggcg gccatcgacg
      661 taagcgtcgt cggaacgaaa tgtcgccact tcaaattgaa agccgttttc gacgactacg
      721 atgacgccaa agtgcgcacc gacagcgtaa gtgcgcggga aaatctgttg cacgatcgcc
      781 ggcgtcgcgt cagtcgcgat gtcgaaatct ttcggcgtct cacggcgcac gatgtcgcgc
      841 acgcagccgc cggcaaaata cgcgatgtgc ccctgctcgc gcagctggtg cgcgatgtcg
      901 cgtgccgttt tctccatcgg gctcacgccg ttgactgtag cggcggtctg ggaccgccgc
      961 acattgacgt tcaggtttat gtcgctgttt cggacgtgct tttggtttgg cgaaaagaat
     1021 taatgatgaa gctcaccacc gcgagcgtga tgcatgaatc ggcgatatta aatgccggcc
     1081 agggatgagc gaacggcacg tgcaagtcga acaggagaaa atcgatgaca tggccgtaca
     1141 gcagccggtc agtgaggtta ccgagcacac cggcgaggag caggcctaac gagagatcgc
     1201 gccagaaatc ccgggagcgc cgccggagca acagcacgac gacgaacagg agcgccagac
     1261 acgacagcgc aacgaagaac tcgttgttat ttttgaatga gccaaaagcc gcgccggtgt
     1321 tggtgatgtt gacgaggctg aagaaacccg gaaccacgat gcgcggttct tccgttccga
     1381 gcgcgccacg gacccaaaat tttgttacct gatccagtgc gtagaggggc agcgacagaa
     1441 agaaaatgaa tttcatgcag gtcgttccac cactcgtgtc atgtcgagcg aggtcgagac
     1501 atctctcatt gtttcaacgg caagagattc ctcgacttcg ctcggaatga caatagctgc
     1561 ttaccgcgct cggttcgcga gcaatgccac cacggcctcg cagcggtcac acagctcggg
     1621 atgcgctttg ctcgcaccga cggttgaacg gtggcgccaa caacgaacac atttttgaaa
     1681 gccggtccgg gtgatcgaca cgctggcttc gctggcttgc tcgacagtca ggtcgctcac
     1741 gataaaaaac tcctccagtt cgttcttgtc gatcttgcgc gtcacatccg aatcggaact
     1801 gagcacgacc gccgcttcga gggagttgcc aatcagtttc tgctgacgcg cctgctccag
     1861 cgcctgacca atgatgccgc gcagccgcaa cagctcagca atcgcctcag tggcttgcct
     1921 tccgcgattc cgcggagttg ggaactcttc caaatgaacc aacgccgtcg caccggagtg
     1981 acgccaggct tcctcagctg tgaaagcgag aatcggcgcg agcaactggc aaagtgcgtt
     2041 gaagatctcg tgcatcgcgg cctgcgtcgc gcggcgccgc ggggaatccg gcgcgtcgca
     2101 atacatccgg tccttggtga tgtcgacgta gagactgctc aggtcgacgg cgcaaaactg
     2161 attgatcgcg tggtaaacct tataaaactc gaaagcctga taagccgcac gacaatcggc
     2221 aacaacttga tccaaccgct ccagaatcca gcgatcgatt aaggtgagat ctgtagccgc
     2281 ggtcgttgac cgcgggccgg cttcagcgac gccggctcca ggaaaatcaa acaggttgcc
     2341 taacaaaatg cgcagcgtgt tgcgaatgcg gcggtaggtg tcgccgagcc gggtgaacat
     2401 ttcctccgag aacgggacgt cgtcggtgta atcgatgctg cttgcccaga gccggacgag
     2461 atcagcgccg tggcggccga cgaaatgccc agcgtccgtt ggcttgtcgt aagtcccgga
     2521 cttggagatt ttctttccat cgagatcgac tacgaagccg tgcgtgacgc aggccttgta
     2581 aggcgcgcga ccgttcaatg cgatgctggt catgagcgac gattggaacc agccgcgatg
     2641 ctggtccgtc gcttccaggt acatgtcggc cggatcgcgt agctcaggat gaatcgcgca
     2701 gactgcctta tgcgagacgc cgctgtcgat ccagacatca atcgtgtcgt tgcgtttggt
     2761 cgtgccctcg ggtagcccga gttgcttcgc aagatcgaca tcatcgagtt cgaaccagat
     2821 gttggagccg cgctcggcca cgagatcagc cagcttgcgg ataatctttg cgtcgagaat
     2881 cgcttcgccg gttttcgaat agaagactgg cagcggcacg ccccaactgc gttgtcgaga
     2941 aatgacccag tcgggccgtg cttccaccgt gccggcgatg cggttctcgc cccagccggg
     3001 aatccatctc acttcgttgt gaatcgcggc gagcgcctgg cggcgcagct cgtcgatacg
     3061 gatgaagaat tgttcgaccg cgcgaaagat gatcggcgtc ttcgaacgcc agcagtaggg
     3121 ataggaatgg tgaaaggttt gttggcctaa caaattgccg cgttcacgca acagattgat
     3181 gatgtcgtgg ttcgcgtcga acacgtactt gccggtcagt tttggcaggc tcgcttccgc
     3241 ggtgaaccgg ccatgatcgt cgaccggaga taagatcgct aatccgttgt tgtagccgag
     3301 ccagtaatcg tcagcgccat ggccaggtgc gatgtgcacc gcgcccgtgc cgctatccat
     3361 cttcacgaaa tccgccagca gcacgacggc ttcccggtcc aggaacgggt ggcgcgctct
     3421 gattttgtcg atcttgtcgc cggtaaatga ggctttcggt tcgccgacgg gatcgaactt
     3481 tgtgtgcgcg caaaattgcg gaacgagttt gtcggcgagg agcaatgttt caaaagcgcc
     3541 gtcgcgcatg aattcctgca cgacgtaaag atccttaggc gacaccgcga tggccatgtt
     3601 cgccggcaac gtccacggcg tagtcgtcca gatggcgatg ctcgctttgt ctttccattc
     3661 accggtcgcg attgggaact tcacgtaa
//
LOCUS       DASTDG010000168        15611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_897702, whole genome shotgun sequence.
ACCESSION   DASTDG010000168 DASTDG010000000
VERSION     DASTDG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 15611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15611
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897702"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFO30_05495"
     CDS             <1..612
                     /locus_tag="VFO30_05495"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HET9418776.1"
                     /translation="LFQGQEFGASAVFRYFSDVGDDELKEAVRKGRFEFLAQFPSVAA
                     PEVQARLPVPFHLSTFAGCKLDWSDRERYSQLYALHRDLIWLRREDARFRKSKPNGVD
                     GAVLGRRSFVLRYFADQPDEERLLIVNLDKQHVFSPAPEPLLAPPDKYEWQTLWTSEA
                     PQYGGTGMPPIATESGWTIFAEAAVALHAVRATKPRPKPKRRQ"
     gene            614..2686
                     /locus_tag="VFO30_05500"
     CDS             614..2686
                     /locus_tag="VFO30_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9418777.1"
                     /translation="MHDLMRNKSTAREPVERVMQWEPANPSREVLLKREWLVTNGLGG
                     YSSGTVSGAVTRRYHGLLVAALAAPLGRVVMWSHVSESLSFGKNDTVSLGAEERAGGQ
                     LELKSAEYLREFRLEDGLPVWVYHVRDLVLEKRVMMPHLQNTVYVTYRILAGDSRPRL
                     ELRPAFHFRHHEISVNKGTSGPYRLSAVADRYEVAAAKPGFPAVRMKVCGEAKFTIGA
                     TRVHQVVYRTEQLRGYAYEGDLWSPGYFDVDLAPDKSATVVASTEKWEITELLSPEEL
                     LTSERRRRRRLLEKTHDRCRDKFAAELALAADQFVITPAGRFEEAARAHAAGDEVRTV
                     IAGYHWFTDWGRDTMISLEGLTLVTGRWLEAGYILRTFANYICDGLIPNMFPDSSKQG
                     KYHTADATLWFFHAVNRYVEITEDQSTLDLLLPSLVDIVDHHLRGTRFNIHVDPADGL
                     LAQGEQGYQLTWMDAKMGDWVVTPRRGKAVEINALWYNALCLVAKWLRENGDARAETY
                     ERHAERARHAFNERFWYADAGYLYDVVDCEGKNGTHDISFRPNQIFAISLENPILDQS
                     RWSSVVDLVGQKLLTPVGLRSLSPDDPEYKPIYSGDLRSRDGAYHQGTVWAWLIGPFI
                     DAWLKVHPNEKERARKFLEVFPTHLSDDGFGTISEVFDAREPHNAGGCIAQAWSVAEV
                     LRTWVKTA"
     gene            2737..3786
                     /locus_tag="VFO30_05505"
     CDS             2737..3786
                     /locus_tag="VFO30_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9418778.1"
                     /translation="MPETAEDLFKQPDVRQELARATGQKAEAKVKAETKDKFWFVTHA
                     LLLGGCAALYFLIGSKFIPLLQAEVDLSRRLLRGFALIVIVIGTGKAVKVYAIGRLQD
                     AVTRFTLERIQNLVVTLLIAVIAVSVIFVNWYAALTALGIGSIIIGLAVQTPMKSFIA
                     WIYILVRRPFQVGDRIKIGDATGDVIDVGYLDTTLWEFGGQYLSTDHPSGRVIRFPNE
                     KVLDEIIWNYSWPLFPYIWNEIKFQIAYQSDLKFVAETMQRIVEEEIGEEMMKRVEVF
                     RDLLARTPVDELEVRSRPRVIFRVDEVTWVDAIVRYLVPPREAGAVKTRLIPKLLSAL
                     NAKPERVMFPKGDAR"
     gene            3824..4825
                     /locus_tag="VFO30_05510"
     CDS             3824..4825
                     /locus_tag="VFO30_05510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9418779.1"
                     /translation="MKLRTIVMTTMSILCGIAANAQQPPSLRPAAKPRPTPEIYAALP
                     NYDEVTATRLQIFLDNCDFGPGKIDGKMGEFFRKALLSYKHAHLMRETGYVDQWLLDQ
                     VPVTFTTYTIRPDDSKFVGHVPGSHAEQATLKWLPYTSLLEYVAERYHSAEDYIVKLN
                     PGKNWEHLQPGDTLTVPNVLPFEVEKIVEQKLPENPAFATRKIYVDTKERLLEVFDND
                     QLVCVFPITPGSKTLPAPIGTWKILAATTWPWFRYDEAMLHYGVRSSEYYNLPPGPNN
                     LVGVLWMGLNKPGIGIHGTNNPETIGRAASHGCIRLANWDAARVKDLVSVGNTVIIF"
     gene            5080..6837
                     /locus_tag="VFO30_05515"
     CDS             5080..6837
                     /locus_tag="VFO30_05515"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF010657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HET9418780.1"
                     /translation="MKEQSGISYSNAAVAATPKHLLQFAVNQRYDDYTSVDHAVWRFI
                     MRQNIFFLREYAHKVYFQGLLNTGISFERIPRIEEMNDILARIGWGAVAVDGFIPPAA
                     FMEFQAYKVLVIACDMRQIHHIEYTPAPDIVHEAAGHAPIIVDREYSNYLQRFGEVGA
                     KAMQSRKDFELYQAIRHLSILKEQPHSDPKEVEEAQKLVEYRQANLGKPSEMALLSRL
                     HWWTVEYGLIGTLENPKIYGAGLLSSIGESVSCLEPDVKKIPYSIDAMNQPFDITTRQ
                     PQLFVCRDFQHLRDVLEEFASQMAYQVGGLEGINKAIECNNVATCEYSSGLQVSGVFD
                     EVIVDQNDSPIYLHTTGKTALAYNNSELPGHGIDYHREGFGSPIGNFRPNELREGEKA
                     KLEFDSGITVEGKIGNILRRDGKTLLISFSHCSAKHGDRVLFDPDWGTYDMAIGDRIT
                     SVFNGAADKDAYHDVALVPKERTIKVPSDAKRKKLENLYAQVRQIRETKKGYERLGEI
                     WETQQAEHPEDWLLSMEIYEILDVTAQQPELKAKIGKFLNEKKATTKDLETLISWGFR
                     LVEYHKQPDFVAAMQASPK"
     gene            complement(6893..7741)
                     /locus_tag="VFO30_05520"
     CDS             complement(6893..7741)
                     /locus_tag="VFO30_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9418781.1"
                     /translation="MISALQSEIGINIIASIIASAVFLVAGFIWGKYKERRRKFGRRL
                     EEYDFYPFTVTRDNFGEFSLKDFRLGMHYFLKNEDYTAARQLIFIGEQNNVREKLEPT
                     EQKVYAQLFEKYDGKKVADDTHEFLENYEHIVRLIGKSFPNTGIEILLHNLADPAHSL
                     CALENNITGRHLRDGTTNLLIDLKRRQLLHEDKLNYELNIGARKFKCTTIPIVRKEYG
                     IVGAICINVDVNYLTEEVLKDRQRIENWFSNLCRTDMQLDENILSKEEYAKALKGKRH
                     FRDETL"
     gene            complement(7881..8573)
                     /locus_tag="VFO30_05525"
     CDS             complement(7881..8573)
                     /locus_tag="VFO30_05525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9418782.1"
                     /translation="MKRYLITGASRGIGRSVAKKLAGLDVTLLLHGRDTIALAETCSA
                     VKSNCARVTSLVHDLATPDGVAKLIAQVGDAPLDLLVNNAGIAIVKPFAEITEVEWSQ
                     TLGVNVTAPFLLSQHFAPRMPPGSSIVNILSIAAKTGFPNWSAYCMSKFALEGFSQSV
                     REELREHKIRVINIYPAATATDIWNAIEGDWPRRKMISPEQVASAVAYALSCPTDVAV
                     ENITISNTGGNL"
     gene            complement(8629..9840)
                     /locus_tag="VFO30_05530"
     CDS             complement(8629..9840)
                     /locus_tag="VFO30_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9418783.1"
                     /translation="MLLTVSKRLEFSSSRRLFRREWTEDENLAAFGPETRARHGTGRN
                     YIAYFVFNGRVDPVTGMLLNISEIKERAGRIVDERFDHKLLNEDNPAFADLPPTAENI
                     VRQLYVDVAPLFSDTDATLAACHVIETAERSATFYSSGACEGNYWFDFSAARQTMSPL
                     ISAAENEKLFGSAASLHGHNYRARLTLRSKKFSPGELLVEHREINACIESLRCELDHR
                     YLNAELAALKNRPMTTETLANYIHERAAKLVPVQRVRLHERADFFAEAWADGNVFLGM
                     QMPFSAAHRLHVATFSDEKNVALFGKCDNPRGHGHTYVAEATLAGTYDERSGTAYDFV
                     ALQNALTDSLEPWQDRHLDLETEDFRDTPSTGENIVRALWPKIDNRVGQQLVRLRLWE
                     TPNNRFTLRRM"
     gene            complement(9982..10524)
                     /gene="folE"
                     /locus_tag="VFO30_05535"
     CDS             complement(9982..10524)
                     /gene="folE"
                     /locus_tag="VFO30_05535"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET9418784.1"
                     /translation="MTKVYRELLDAIGEDAERQGLVKTPARAARAMEFLTQGYRQSVE
                     EIVNNAIFDSEASEIILVKDIELYSLCEHHLLPFIGRAHVAYIPNGKVVGLSKVARIV
                     DVFARRLQIQENLTTQIAESLMDCLQPNGVAVVVEAKHLCMMMRGVEKQNSVMKTSCL
                     LGSFKEDARTRSEFLSLLKE"
     gene            complement(10636..11820)
                     /locus_tag="VFO30_05540"
     CDS             complement(10636..11820)
                     /locus_tag="VFO30_05540"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9418785.1"
                     /translation="MPYYQKLGETPRKHHIWFHRNGQGPSYKNEGIAYEHVITTEGFN
                     EAYSIMYHLRPPTRVRNVKLLKCEELKKVTDSPLRHHHLKTAKIPRRGDLYTGRIPVL
                     FNQDVIAYRARPEKAYGEFEYYRNGGADEIIFVFKGGGTLESVFGKHRYRAEDYIVIP
                     RGITYRLVPDKVEEEDYLVLESVGPVRIPQRYLNKEGQIKMGSPYSERDLHGPTELNT
                     TDKDEDTEILVKDGPRWTCVTLAHHPFDVVGWDGYVYPFTFNAQDFEPITGTVHQPPP
                     IHQTFEIRGYVVCTFAPRLLDTHPEAVKIPWVHDNVEADEVLFYVRGNFGSRRGIEPA
                     SITLHPRGIPHGPHPGTIIASKNATRTEELAVMFDTQHTLELTEQAVALDDEKYPQSW
                     LD"
     gene            complement(11838..12971)
                     /gene="hppD"
                     /locus_tag="VFO30_05545"
     CDS             complement(11838..12971)
                     /gene="hppD"
                     /locus_tag="VFO30_05545"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET9418786.1"
                     /translation="MPTTIAAPKSAPAAEQDFLPLEGTDYIEFYVGNAKQAAHFYKTV
                     FGFQSLAYSGPETGVKDRASYAIRQNKLTFVLTTPIRANNPVADHIYKHGDGVKAIAL
                     QVKDATSAWQETTKRGGKSYLEPKTLKDEHGKVVMSGIHTYGEVVHLFIERDDYTGPF
                     MPGFVKWESPYRASETGLQHVDHCVGNVGWNQMNPWVKFYEEVMGFRNILTFDDKDIS
                     TEYSALMSKVMSNGNGFVKFPINEPAEGKKKSQVEEYLEFYDGEGVQHIAMATNNIIE
                     TVADLQNRGLEFLKVPSTYYDTLLDRVGHIDEELNALKRLGILVDRDDEGYLLQIFTK
                     PVEDRPTLFFEIIQRKGAKSFGKGNFKALFEAIEREQAARGNL"
     gene            complement(13257..15023)
                     /locus_tag="VFO30_05550"
     CDS             complement(13257..15023)
                     /locus_tag="VFO30_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9418787.1"
                     /translation="MKITLRNTAIIFATLALSVSLHAQNGAPTKYNEMTIAQLQAQMQ
                     AGTLTSVALTKFYLDRIAALDKNGTDGGLNSVIELNPDALAIAQHADDLRAQGKVRGP
                     LHGIPVLLKDNIDTGDKMQTTAGSFALFGKPAPQDSTVAANLRSGGAVILGKTTLSEW
                     ANFRSFESTSGWSGRGGQCNNPYALDRNPCGSSSGSGAAASANFAAVSLGSETDGSIV
                     CPGNANGVVGLKPTVGLTSRAGVVPISHTQDTVGPHGRTVADAAATLGVIQSRTFDGR
                     DPATGGVPLGWQGTGKTRPNNIPTDYTQFVDPNGLKGARLGLTRSGIEGFDPFNPTPQ
                     PIIDAFNAAAEKLSAAGATVIDLDKQGFTFPSADGEFLVLCYEFRQDVRAYFATRVGV
                     PVAGGTLSTAIQFNRDHANVEMPFFNQDIFVLCAALAQGPATPQPIFGGLTYNQALKI
                     DHDAGVNGIDKALKKFNLDAIVSFTDNPAWETDLLYSDTGRFYFASSGLAAAPGYPII
                     QVPAAMVFGTPMGISFLGTAFSEPTLIKLASGYEAFTQVRAHNLPQFLDNTPSHHIEG
                     TTPVQDTDTVNPANAKKKPQGM"
     gene            complement(15382..>15611)
                     /locus_tag="VFO30_05555"
     CDS             complement(15382..>15611)
                     /locus_tag="VFO30_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418788.1"
                     /translation="LLPVTKDAVTGAEILRNLAITCAWADEKDLAIKQLDEALQLPSP
                     ISYGQLRLHPWWDPLRQDARFEQIIASVAPK"
BASE COUNT         3292 a         4445 c         4508 g         3366 t
ORIGIN      
        1 ttgtttcaag gccaggaatt tggcgcttcg gcagtgtttc gatatttcag cgatgtgggc
       61 gatgacgaat tgaaagaagc agtccgcaag ggccgcttcg agtttctggc ccaatttcca
      121 tcagtggcag cgcccgaggt gcaagcgcgc ctgccggtgc cgttccatct cagcacgttt
      181 gcaggctgca aactcgactg gtcggaccgc gagcgctatt cgcaactcta cgccttgcac
      241 cgcgatttga tctggctgcg tcgtgaagac gcgcgttttc gtaagtcgaa gccgaatggt
      301 gttgatggcg ccgtgctcgg ccggcgctca ttcgtcctgc gatattttgc cgatcagccg
      361 gacgaggaac gattactgat cgtgaatctc gacaaacagc acgtcttcag ccccgcgccg
      421 gagccgttac tggcgccgcc ggataaatac gaatggcaga cgctctggac aagcgaagct
      481 ccacagtacg gcgggacggg catgccgccg atcgcgacgg aaagcggctg gacgattttc
      541 gccgaagcgg ccgtcgcgct gcacgccgtc cgcgcgacca agccgcgacc gaaaccgaag
      601 cggcgccagt aaaatgcacg acctgatgcg aaacaaatcg accgcgcgcg agccggtcga
      661 gcgcgtgatg cagtgggagc cagcgaatcc ttcgcgcgag gttttgctga agcgcgaatg
      721 gctcgtcacc aacgggcttg gcggttattc cagcggcacc gtgtccggcg cggtgacgcg
      781 tcgctatcac ggcttgctcg tcgcggcatt ggccgcgccg ctcggacgcg tcgtgatgtg
      841 gagtcatgtc tcggaaagcc tttccttcgg taaaaacgac accgtttcgt taggcgcaga
      901 ggagcgcgcc ggcgggcaac tcgaattaaa aagcgcggag tatctgcgcg agttccggct
      961 cgaggacggc ctgccggtct gggtgtatca cgtccgcgat ctcgtgctcg agaaacgcgt
     1021 gatgatgccg cacctgcaaa acacggttta cgtcacctac cgcattttag ccggcgattc
     1081 acgtccccgt ctcgagctgc ggccggcgtt tcattttcgg catcacgaga tttcggtaaa
     1141 caagggaact tccgggccat atcgcctgtc tgccgtagcg gatcgctatg aagtcgcggc
     1201 cgccaaaccg ggttttccag cagtgcggat gaaagtttgc ggtgaagcga aattcaccat
     1261 tggcgcgacc agggttcacc aggtggttta ccgcaccgag caactgcgcg gctatgccta
     1321 cgagggcgac ctttggagtc caggatactt tgatgtcgat cttgcgccgg acaaatccgc
     1381 caccgtcgtc gcttcgaccg aaaaatggga gatcaccgaa ttactcagtc ccgaggaatt
     1441 acttacctcg gagcggcgac gacggcggcg tttactggag aaaacgcacg atcgctgccg
     1501 tgacaaattt gcggctgagc ttgccctcgc tgctgaccag ttcgtcatca cgccggccgg
     1561 ccgattcgag gaagcggcgc gcgcgcacgc cgcgggcgac gaggtgcgga cggtgatcgc
     1621 cggttaccat tggttcaccg actggggccg cgacactatg attagcctcg aagggctaac
     1681 gttggtcacc ggtcgctggc ttgaggccgg ttacatcctg cgcactttcg ccaattacat
     1741 ctgcgatgga ttgatcccga acatgttccc cgacagctcg aagcagggga aataccatac
     1801 tgccgacgcg acgctgtggt ttttccacgc ggttaatcgc tatgtcgaga tcaccgaaga
     1861 ccagtcgaca ctcgatttat tgctgccgtc gttggtcgac atcgtcgacc atcatctgcg
     1921 cggcacgcgc ttcaacattc acgtcgatcc tgccgatggg ctgctcgcgc aaggcgaaca
     1981 aggttaccag ctcacctgga tggacgcgaa aatgggcgat tgggtggtga cgccgcgcag
     2041 gggcaaagcc gtcgagatta acgcgctttg gtataacgcg ctctgtctcg tcgcgaaatg
     2101 gttgcgcgaa aacggcgacg cgcgcgccga aacctacgag cgccatgccg aacgcgcgcg
     2161 tcacgcgttt aacgagcggt tctggtacgc agacgccggg tacctttacg acgtggtcga
     2221 ttgcgagggg aaaaatggaa cgcacgacat ttcgtttcgg cctaaccaga tcttcgctat
     2281 ttcgctcgaa aaccccattc tggatcaatc gcgctggagc tcagttgtcg atcttgtcgg
     2341 gcaaaaactg ctgacgccgg tcggcctgcg ctcactttcg ccggacgatc cagaatataa
     2401 gccgatttac tccggcgatc tgcgctcgcg cgatggcgcc tatcaccagg gcacggtctg
     2461 ggcatggctc atcgggccgt tcattgacgc gtggttgaag gttcatccaa acgaaaaaga
     2521 gcgcgcccga aaattcctcg aagtcttccc gacccatctg agcgacgatg gtttcggcac
     2581 aatcagcgag gtgtttgacg cgcgggagcc gcacaatgcc ggcggctgca tcgcgcaagc
     2641 gtggagtgtg gccgaggtcc tgcgcacctg ggtgaaaacg gcctaacgag ccaagatcga
     2701 caatcgctcg ccgcggctgc tgcgatttat taattgatgc ctgaaaccgc ggaagacctc
     2761 ttcaaacagc ccgacgtgcg gcaggaactg gcccgcgcca ctggacagaa ggcggaggcg
     2821 aaagtcaaag ccgagacaaa agacaaattc tggttcgtta cccatgcatt gcttctcggc
     2881 ggctgcgccg cgttgtattt tctcatcggc tcaaagttca tcccactttt gcaagcggaa
     2941 gtggatctga gccgccgcct ccttcgcggc ttcgcgctca ttgtcatcgt catcgggacg
     3001 ggcaaggcgg tgaaagtgta tgcgatcgga cggcttcaag acgccgttac ccgattcacc
     3061 ctcgaacgga tccagaatct ggtggtgact ctgttgatcg cagtcatcgc ggtgtcggtg
     3121 atttttgtga attggtacgc cgcgctcaca gcactcggca tcgggtcgat catcatcggt
     3181 ctggccgtgc aaacgccgat gaaaagtttc atcgcctgga tttacatcct ggtgcggcgg
     3241 ccgtttcaag tcggagatcg gatcaaaatc ggcgacgcca ccggcgacgt catcgatgtc
     3301 ggctacctcg acaccacgct ctgggaattt ggcgggcaat atctttccac cgatcatccc
     3361 agcgggcgcg tgattcgttt tccgaatgaa aaggtgctgg acgagatcat ttggaattat
     3421 tcctggccgc tgtttcctta catctggaac gagatcaaat ttcaaatcgc ttaccaaagc
     3481 gatctgaagt ttgttgccga gacaatgcaa cgaattgtcg aagaagagat cggcgaggag
     3541 atgatgaagc gggtggaagt ctttcgtgac ttgctcgccc gcacaccggt cgatgaattg
     3601 gaagtgcgtt cccggccgcg tgtcatcttt cgcgtcgatg aagtcacgtg ggtcgacgcc
     3661 attgtgcgct atttggtgcc gccgcgcgaa gcgggcgcgg tcaaaacgcg cctcatccca
     3721 aaactgctct ccgcgctcaa cgccaagcct gaacgagtga tgttcccgaa aggcgatgcg
     3781 cggtagattg cgcgattcgg agcgtaaaac ctaacttcgc caaatgaagt tacgaacgat
     3841 tgtaatgacg acgatgtcga tcttgtgcgg catcgcagca aatgcgcagc aaccgccgtc
     3901 cctgcgaccc gcggcgaaac cgagaccaac tccagaaatt tacgcggccc tgccgaatta
     3961 cgacgaggtc accgcgacgc ggctccagat ttttctcgat aattgcgatt ttggcccggg
     4021 aaagatcgac ggcaaaatgg gggaattttt tcgcaaggcg ctcctctcct acaaacatgc
     4081 gcacctcatg cgcgagaccg gctacgtcga ccaatggttg ctcgatcagg tgccggtcac
     4141 gtttacgacg tacacgatcc gtcccgatga ttcaaaattc gtcggccacg tgccaggcag
     4201 ccacgccgag caggcgacac taaagtggct gccttacaca tcgctgctcg agtacgtggc
     4261 ggaacgctat cattccgccg aggattacat cgtaaaactg aatcccggca aaaattggga
     4321 gcacttgcag ccgggcgaca cgctcactgt gccgaacgtg ctgccgttcg aagtcgaaaa
     4381 aatcgtggag caaaaattgc cggagaaccc ggctttcgcc acgcgcaaga tttacgtcga
     4441 cacaaaggag cgcctgctcg aggtattcga caacgatcag ctcgtctgcg tttttccgat
     4501 cacgcctggc tccaagacgc tgccggcacc gatcggcacg tggaagattc tcgccgcgac
     4561 gacctggccc tggttccgct acgacgaggc gatgctgcac tatggtgtgc gcagtagcga
     4621 gtattataac ctgccgcccg ggccgaacaa cttggtcggc gttctctgga tgggcctcaa
     4681 caagccaggc atcggcattc acggaaccaa taatccggag acaatcggcc gcgccgccag
     4741 tcacggctgc atccgtctgg cgaactggga tgctgcgcga gtcaaagacc tggtcagcgt
     4801 cggcaatacg gtcatcattt tttgatgttc aaatgtggga gggacctcag cgtcccgata
     4861 tcgcccgtct tgctcgggct cgcggcacta aggcggctcc cgcagtaaat ttagatcgac
     4921 atgatggatg ccaatgagcg ccaagtttat cgggcggcca ttctgcgaac gcgccggcaa
     4981 aagtagcgca tgcgtcccgc ttgcatttaa tcgattcgca agccagaggc ttgcgctact
     5041 ttagggcgag tcgtacgatt attgttgagg aggatgccga tgaaagagca aagcgggatc
     5101 agctacagca acgcggctgt cgccgcgacc ccaaaacatc ttttgcagtt tgccgtcaat
     5161 cagcgctacg acgactacac gtcagtcgat catgccgttt ggcggttcat catgcggcag
     5221 aacattttct tcctccgcga atacgcgcac aaagtttatt tccaaggcct gctcaacacc
     5281 ggcatttcgt tcgaacggat tccgcgcatc gaggagatga acgacatcct cgctcgcatc
     5341 ggttgggggg cggtggcagt agacggcttc attccgccgg ctgcgttcat ggagttccaa
     5401 gcttacaagg ttctggtgat cgcctgcgac atgcgccaga tccaccacat cgaatacacg
     5461 ccggcgccgg acatcgtgca cgaagcggca ggacacgcgc cgatcatcgt cgatcgcgaa
     5521 tattcaaatt acctgcagcg cttcggtgaa gtaggcgcga aagcgatgca gtcacgcaaa
     5581 gatttcgagc tctaccaagc gatccggcat ttgtcgatct tgaaagagca gccccacagc
     5641 gacccgaaag aagtcgagga agcacagaag ctcgtcgagt atcggcaggc gaaccttggc
     5701 aaaccatcgg agatggcgct gctctcgcgg ctgcactggt ggacagtcga gtacggactc
     5761 atcggcacgc ttgagaatcc gaagatttac ggcgccgggc tgctctcctc gatcggcgaa
     5821 tcggtctcat gcctggagcc ggacgtgaag aagatcccct actccatcga cgcgatgaac
     5881 cagccgttcg acatcactac gcgacagccc cagctctttg tctgccgcga tttccagcat
     5941 ctgagggacg tgctcgaaga attcgccagc cagatggctt accaggtcgg cggcctcgaa
     6001 ggaatcaaca aagccatcga atgcaacaat gtagccacgt gcgagtattc gagcgggctg
     6061 caagtcagcg gcgtcttcga cgaagtgatt gtcgatcaaa acgacagccc gatttatctg
     6121 cacacgacgg gcaaaaccgc cttggcctac aacaacagcg agcttccggg ccacggcatc
     6181 gattaccatc gggagggatt cggctcaccg attggcaact tccggcctaa cgaattgcgg
     6241 gaaggcgaaa aggcgaagct ggagttcgac agcggcatca ctgtcgaagg aaagatcggc
     6301 aacatactgc gacgcgacgg caaaacgctg ctcatcagct tttcccattg cagcgccaag
     6361 catggcgatc gcgtcttgtt cgatcccgat tggggcacat acgacatggc gatcggcgat
     6421 cggattacat cggtgttcaa tggcgccgcc gacaaggacg cttaccacga tgtggcgctt
     6481 gtgccgaaag agcgcacgat caaggtaccg tcagatgcga aacgcaagaa actggagaac
     6541 ctctacgcac aggtgcgaca gattcgcgag acgaaaaaag gttacgaacg gcttggcgaa
     6601 atctgggaaa cacaacaggc tgaacacccc gaggactggc ttttgtcgat ggagatttac
     6661 gagatcctcg acgtcaccgc gcaacaaccc gaattgaaag ccaagatcgg caagttcctg
     6721 aacgaaaaga aagcgacgac gaaagacctc gagacgctga tcagctgggg gttccggctg
     6781 gtcgaatatc acaagcaacc ggatttcgtc gcggcgatgc aggcgtcgcc gaagtaaggt
     6841 agcgcaagcg tctcgcttgc atttcggcgg ctcgcaagcc gggacgcttg cgctacaatg
     6901 tttcgtccct gaagtggcgt ttgcccttca gtgccttcgc gtactcctcc ttgctcagga
     6961 tgttctcgtc gagctgcatg tcagtgcggc acagatttga gaaccagttc tcgatgcgtt
     7021 gtcgatcctt cagcacttcc tccgtgagat agttcacatc cacattgatg cagatggcgc
     7081 cgacgatgcc gtattcctta cgcacgatcg ggatggtcgt gcacttaaac ttccgcgcgc
     7141 cgatgttcag ctcgtaattc agcttgtcct cgtgcagaag ctgccggcgt ttgagatcaa
     7201 tcaacagatt cgttgtgccg tcgcgcaggt gccgccccgt gatgttgttc tcgagcgcac
     7261 agagcgagtg cgccggatca gccagattgt gcagcaagat ctcgatcccg gtatttggga
     7321 atgatttgcc gatcaagcgc acgatgtgtt cgtagttctc caaaaactcg tgcgtgtcat
     7381 cggcgacctt tttgccgtcg tacttctcga aaagttgcgc gtaaaccttc tgctccgtcg
     7441 gctcgagttt ttcgcgcacg ttgttttgtt caccgatgaa gatgagctgc cgcgcggccg
     7501 tatagtcctc gtttttgagg aagtaatgca tcccgagccg gaaatctttc aggctgaact
     7561 cgccgaagtt gtcgcgcgta acggtgaacg gatagaaatc gtattcctcg aggcggcggc
     7621 caaatttgcg acggcgttcc ttgtatttgc cccaaatgaa acccgcgacc aggaaaaccg
     7681 ccgaggcaat gatcgatgcg atgatgttga tcccaatctc gctttgcaaa gcgctgatca
     7741 tagaactcgg aacatagggc tatgccctgt gtgcccagcg gacattttgt ccgctgccgt
     7801 cgctgattca gcggagtaaa actccgctgg gcgcacagac tgaaagtcta tgttcctgtc
     7861 gctaactttg cgcccgtcga tcacaaattt ccgcccgtgt ttgagattgt aatgttttcc
     7921 accgcgacat ccgtcggaca cgacaaggcg taagcgaccg cggacgcgac ttgctcgggc
     7981 gagatcattt tgcggcgcgg ccagtcgcct tcgattgcgt tccaaatgtc ggtggcggtg
     8041 gccgctggat agatgttgat gacgcgaatc ttgtgctcgc gcagctcctc gcgcactgac
     8101 tgcgaaaatc cttccagcgc gaacttgctc atgcaatacg cgctccagtt cgggaagccg
     8161 gtcttggccg cgatcgagag aatgttcacg atgctcgagc ccggcggcat gcgtggggcg
     8221 aaatgctggc tcagcaaaaa cggcgcggtg acattgacgc cgagcgtttg agaccattcc
     8281 acttcggtga tctccgcgaa cggttttaca atcgcaatgc cggcattgtt cacgagtaaa
     8341 tcgagcgggg cgtcgccgac ttgtgcgatc aacttggcaa ctccgtcggg cgtcgccagg
     8401 tcatgaacga gtgacgtgac acgcgcgcag tttgatttga ccgcgctgca agtttcagcc
     8461 aacgcaatcg tgtcgcgccc gtgcaaaagc aacgtcacgt cgaggccggc aagtttcttc
     8521 gcaaccgaac ggccgatgcc gcgactcgcg cccgtaatga gataacgctt catcaggtag
     8581 agcaagcttc caggttgctc ctatttagac gcaagccgga ggcttgcgct acattcgacg
     8641 cagcgtaaac cgattgttcg gcgtctccca caaacgcagc cgcaccagtt gctggcccac
     8701 gcgattgtcg atcttgggcc agagagcgcg cacgatgttc tcgcccgtcg agggtgtgtc
     8761 gcgaaaatct tccgtctcca aatcgagatg tcgatcttgc cacggttcaa gcgagtctgt
     8821 gagcgcgttt tgcaaggcaa cgaaatcgta agcagtgccg ctccgctcgt catatgtgcc
     8881 ggcgagcgtc gcttcggcga cgtatgtgtg cccatgaccg cgcgggttat cgcactttcc
     8941 aaatagcgcc acgtttttct cgtcggaaaa cgtcgccaca tgcagccgat gcgcggcgct
     9001 gaagggcatt tgcatgccga gaaaaacgtt cccatccgcc caagcttccg cgaaaaaatc
     9061 ggcgcgttcg tgcaaccgca cgcgctgaac gggcacgagc ttcgccgcac gctcatgaat
     9121 gtagttcgcc agggtctcgg tcgtcatcgg ccggttcttc agcgcggcga gctctgcgtt
     9181 caagtaacga tgatccagct cgcagcgtag cgactcaatg cacgcgttga tctcgcgatg
     9241 ctcgaccagt agctcgcctg gtgaaaattt tttggaccgc aaggtcagcc gcgcgcgata
     9301 attgtgtcca tgcagagaag cggcggagcc gaatagtttc tcgttctcgg ctgcgctaat
     9361 cagcggcgac atcgtttgtc gcgcggccga aaagtcgaac cagtaattcc cctcacacgc
     9421 gccgctggag taaaatgtcg cgctgcgctc tgccgtctca attacatgac aggcagcaag
     9481 cgtcgcgtcg gtgtcggaga ataggggtgc aacatcgaca tacagttgac gcacaatgtt
     9541 ttccgccgtc ggcggcaggt cggcaaacgc cggattatcc tcattgagga gcttgtggtc
     9601 gaagcgctcg tcaacgatcc gaccggcgcg ctccttgatt tcgctgatgt tgagcagcat
     9661 gccggtgacc ggatccacgc gtccattgaa aacgaaatac gcaatgtaat tacgaccagt
     9721 gccatgtcgt gcccgagttt ccgggccgaa cgcggcgaga ttttcgtcct cggtccattc
     9781 acgccgaaac agccgccgcg acgatgagaa ctcaaggcgt ttgctgaccg tcaggagcat
     9841 acacgacaaa catattatac ggattttgcg ccgcaggttg cttgcaaaac gccgcgccgt
     9901 cggccctcac ctcaatcctc tccctgaagg agaggaggac gcgccagcgc cgggtgaggg
     9961 ctcgtgccca cgaggttgac actactcttt gagcaacgag aggaactcgg agcgcgtgcg
    10021 cgcgtcttct ttgaaagagc cgagcagaca ggaagttttc atcaccgaat tctgcttttc
    10081 gacgccgcgc atcatcatgc acaggtgctt tgcctcgacg accacggcga cgccgttcgg
    10141 ctgcaaacaa tccatcagcg actcggcgat ttgggtggtg agattttcct ggatctgcaa
    10201 acgccgcgcg aacacatcga caatccgcgc caccttcgag agaccgacca ccttgccgtt
    10261 cgggatgtaa gcgacgtgag ctcggccgat gaacggcagc agatgatgtt cgcagagcga
    10321 gtaaagctca atgtctttta ccaggatgat ctcgctcgcc tccgaatcga agatcgcgtt
    10381 attgacgatc tcttcgacgc tttggcgata accttgcgtc agaaactcca tagctcgcgc
    10441 cgcgcgtgcc ggtgttttca ccaagccctg acgctcggcg tcttccccga tcgcatcaag
    10501 cagttcgcga taaacttttg tcaatcgtcc aatctgcccc ggcggcaacg gctcggtgta
    10561 ccgcgtcagt cggtcgtcag tttcgatttc agaggtggac gtgcgcaaga tcaacgcttc
    10621 taagatcgac aattgttaat cgagccagct ctgcggatat ttctcgtcat ccagcgcgac
    10681 cgcctgttcg gtcaactcga gcgtgtgttg cgtgtcgaac atgacggcga gttcctcggt
    10741 gcgtgtcgca ttcttgctcg cgatgatcgt gcccggatgc ggcccgtgcg gaatgccgcg
    10801 cggatgtagg gtgatcgacg ccggctcaat gccgcggcgc gacccgaaat tcccgcgcac
    10861 gtaaaacaaa acttcgtcgg cttcgacgtt gtcgtgcacc cacgggatct tgaccgcctc
    10921 gggatgtgta tcgagcagac gcggcgcgaa cgtgcagaca acatagccgc gaatttcgaa
    10981 cgtctgatga atcggtggcg gttggtgcac tgtgccggtg atcggctcga aatcctgcgc
    11041 attgaaggtg aatggataaa cgtagccgtc ccaaccgacg acatcgaagg gatgatgtgc
    11101 cagcgtcacg cacgtccaac gcggcccgtc tttgaccagg atttcggtgt cctcatcctt
    11161 atccgttgtg ttgagctcgg tcggcccgtg caggtcgcgc tcggaataag gcgagcccat
    11221 tttgatctga ccttctttgt ttagatagcg ctgcgggatt cgcaccggcc cgaccgattc
    11281 gaggaccaag taatcctcct cttcaacttt atccggcacg agtcgataag tgatgcctcg
    11341 cggtatgacg atgtaatcct cggcgcgata ccgatgtttg ccgaaaacgg attcaagcgt
    11401 gccgccgcct ttgaagacaa agattatctc gtcggcgccg ccgttgcggt aatactcgaa
    11461 ttcaccatag gctttttccg gtcgcgcgcg ataggcgatg acgtcctggt tgaacaaaac
    11521 cgggatgcgc ccggtgtaga ggtcaccgcg ccggggtatt ttcgcggtct tgaggtggtg
    11581 atggcgcagc gggctatcgg tgactttctt gagctcttcg cacttcagca gcttgacatt
    11641 gcgcacgcgc gtcggcgggc gcaggtggta catgatggaa taagcctcat taaagccttc
    11701 cgttgtgatc acgtgttcgt aggcgatacc ttcgttttta tacgacggcc cctgaccatt
    11761 gcgatgaaac cagatgtgat gcttgcgtgg cgtctcaccg agtttttggt agtacggcat
    11821 tgtcgatctt acctccttta caaattaccg cgtgcggcct gttcgcgctc gatcgcctca
    11881 aacagcgcct tgaagttgcc tttgccgaag ctcttcgcgc ctttgcgttg aatgatctcg
    11941 aagaacagcg tcggccgatc ttccactggc ttggtgaaga tttgcagcag ataaccttcg
    12001 tcgtcacgat cgaccaaaat cccgagacgc ttcaacgcgt tgagttcctc atcgatgtgg
    12061 ccgacgcggt cgagcaacgt gtcatagtag gttgatggca ctttgaggaa ctcgagcccg
    12121 cgattttgca gatcggccac ggtctcgatg atattgttcg tggccatggc gatgtgctgc
    12181 acgccttcgc catcgtagaa ctcgaggtat tcctcgactt gcgatttctt tttgccctcg
    12241 gccggctcgt tgatcggaaa tttcacgaag ccattgccgt tgctcatgac cttgctcatg
    12301 agcgccgagt attcggtgga aatatctttg tcgtcgaagg tcaggatgtt tcggaaaccc
    12361 atcacctcct cataaaattt cacccacggg ttcatctggt tccaaccgac attgcccacg
    12421 caatggtcga cgtgctgcaa accggtttcg ctcgcgcgat atggcgactc ccatttaacg
    12481 aagcccggca tgaacggccc cgtgtaatcg tcgcgctcga tgaacaaatg cacgacctcg
    12541 ccgtaagtat ggatgccgct catcaccact ttgccatgtt cgtccttcaa tgttttcggc
    12601 tcgagataac ttttgccgcc gcgcttggtt gtttcctgcc aggccgaggt cgcgtccttc
    12661 acttgcagcg caatcgcctt cacgccgtcg ccatgtttgt agatgtgatc cgcgaccggg
    12721 ttatttgctc ggattggcgt agtcaggaca aaagtgagtt tgttctgccg aatcgcatag
    12781 ctcgcgcgat ccttcacgcc ggtctcgggc ccgctatagg cgagactttg gaaaccgaaa
    12841 acggttttgt aaaaatgcgc cgcctgcttg gcgttgccga cgtagaactc gatgtaatcc
    12901 gtgccctcaa gcggcagaaa atcttgctcc gctgccggag cgctctttgg agcagcaatt
    12961 gtcgttggca taatccacct cccggtctgc gctctgcgcg cgctattcaa ctataagatc
    13021 gacaatcacg agtcgcaagg atgcggcggc gcgtgattta cttcgatgcg ccgtcttttg
    13081 gcgcgagagg cgacgatttg cgcggatcgc cgctacggag aagatgcagt cgaacaagct
    13141 gcgtagcacg gacgcagcaa gccaagtttc ctggcgcacc ttgtgaagcc gatcagtttc
    13201 gatcggctcc gccggtcgct ggaatagttg ctcgcgctgg gctgagcggc cgcccgttac
    13261 ataccttgcg gctttttctt ggcgttcgcc gggttgaccg tgtcggtgtc ctgcaccggc
    13321 gttgtgccct cgatgtgatg ggatggcgtg ttatcgagga actgtggcaa gttatgcgcg
    13381 cgcacctgcg tgaatgcctc gtaacctgag gcgagcttga tcaacgtcgg ttcactgaaa
    13441 gctgtgccca ggaagctgat gcccatcggc gtgccgaaga ccatggcggc cggcacttgg
    13501 ataatcgggt aaccaggcgc agcagccaga cctgaacttg cgaagtagaa tctgccggta
    13561 tcgctgtaga gcaggtccgt ctcccaagcc ggattgtctg tgaaagaaac aatcgcatcg
    13621 aggttgaatt tcttgagcgc tttgtcgatg ccattgacgc cggcgtcgtg atcgattttc
    13681 agcgcctgat tgtaagtcag cccgccaaaa atcggttgcg gagtagctgg tccctgagcg
    13741 agcgccgcgc aaagaacgaa gatgtcctgg ttaaagaagg gcatctccac gttggcatgg
    13801 tcgcggttga actggatggc ggtggaaagc gtcccgcctg ctacgggaac gccaacgcga
    13861 gtagcaaaat acgctcgtac gtcctgtcga aactcgtagc acaacaccag aaattcgccg
    13921 tcggctgacg ggaaggtgaa cccttgcttg tcgagatcga ttacggtggc gccggccgca
    13981 ctgagctttt ccgccgctgc gttaaacgcg tcgataatcg gctgcggtgt ggggttgaat
    14041 ggatcgaatc cctcgatgcc tgaccgggtc aatccaagtc tcgcgccttt gagcccattg
    14101 ggatcgacga actgagtgta atcagtcgga atattgttcg gacgtgtttt gcccgtcccc
    14161 tgccacccaa gcggaacgcc gcccgtcgcg ggatcgcgtc catcaaaggt acggctctga
    14221 atgacgccga gcgtggcagc tgcatcagct accgtgcgcc catgtgggcc gaccgtgtct
    14281 tgcgtgtgcg aaatcggaac aacaccggcg cggctcgtga gaccaacagt aggtttgagg
    14341 ccgaccacac cgttcgcgtt gcccgggcag acgatgctgc cgtcggtctc gctgcctaat
    14401 gagacggcgg cgaaattcgc cgaggcagcc gcgcccgacc ccgagctgga gccgcatgga
    14461 ttgcgatcga gcgcgtaggg attgttgcat tgaccgccgc gccctgacca gccgctggtc
    14521 gattcaaagg aacggaaatt tgcccattcc gagagagtcg ttttgccaag aataacagcg
    14581 ccacccgaac gcagattggc tgcgaccgtg gaatcctgtg gagcaggttt gccgaacagt
    14641 gcgaatgaac cagcggtcgt ctgcattttg tcaccggtgt cgatgttgtc cttgagcagg
    14701 accggaatgc cgtgcagcgg gccacgcact ttgccttgcg cgcgcagatc atcggcgtgt
    14761 tgtgcgatgg ccagcgcgtc aggattcagc tcaatcacgg aatttagtcc gccgtcggtt
    14821 ccgttcttat cgagcgcggc gattcggtcg aggtagaact tggttagcgc gacggaagtg
    14881 agcgttccgg cctgcatctg cgcctgtaat tgcgcgatgg tcatttcgtt gtatttggtc
    14941 ggcgcaccgt tctgggcgtg cagggacaca ctcagggcaa gtgtggcgaa tatgatagct
    15001 gtatttcgta gtgtaatttt catggtttgt gcttttcgag aagacccgcc ctctagcaaa
    15061 ttggcgcgct cagcacaagc actaaaaaat acagtgttta ggggaaaaat tctgcaagcc
    15121 aacaatggcc actccgagcc gcggctgaat gctgtgatcg caacagcagc acaccgatga
    15181 ttaagaaatc cagcttgcga ccggaaatcc gacaccgatc aactgtacga ccaaccgtcc
    15241 cgcccagttt gcagtttcgc agaaaggaaa gacctgcgtc gcgacctgaa cgagcagcca
    15301 cccgatcgct gcgtacggaa tggcaacgtt gtaaaagttg cgccgcgtca gctcgccgaa
    15361 caaattctgc gaggtaattc gttactttgg tgcgacggag gcgatgattt gctcgaagcg
    15421 cgcatcctgg cgcagtgggt cccaccacgg atgcaagcgc aattggccat acgaaatcgg
    15481 gctgggtagt tgcagcgctt cgtccagttg tttgatcgcc aaatccttct cgtcggccca
    15541 cgcgcaggtg atagccagat tcctgagtat ctccgcgcca gtcacggcat ccttggtaac
    15601 gggcaaaagc t
//
LOCUS       DASTDG010000169         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_797634, whole genome shotgun sequence.
ACCESSION   DASTDG010000169 DASTDG010000000
VERSION     DASTDG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797634"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFO30_05560"
     CDS             complement(<1..939)
                     /locus_tag="VFO30_05560"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET9418789.1"
                     /translation="MTVATSVGTDTMPEMEAADWPSLSDQELLQRRISALGLRLEGTP
                     LEPLIRQLYDELSAKGLTFHPPCHVGDEWFVPIGIPAIFIPFFLVHDRLRALERTMML
                     EVEGGTPEWFMKLMRHEAGHAYMYAYRLTRKKKWQELFGQTSREETPDTYRPRPFSRG
                     YVMHLEDWYAQSHPDEDFAETFAVWLTPGLDWRKRYSRWRALQKLEYVDELMRSLAGK
                     PPVHMPEYRVADYDCLNVKLKTYYARKRKLYEDTYPDFYDADLRQLFGAPAGIKASVY
                     LQRRRRRILNAVCQWTNEKKFRVNQLLARLTRRCDQL"
     gene            1044..1535
                     /locus_tag="VFO30_05565"
     CDS             1044..1535
                     /locus_tag="VFO30_05565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418790.1"
                     /translation="MHDIFRIRRATVQDADVIAWHRARMFQDMGDVSDDGFEILRAKA
                     RLRLKEWLESGDYIGWLATPADKPETVVGGAGIQLQPILPRPRDASTIGEGRQGTIVN
                     VFTEPQWRRRGVAGGLLKEIIAWSKNEGLDRLLLHASDDGRSVYERLGFIAGNEMYFA
                     GVS"
     gene            complement(2288..3040)
                     /locus_tag="VFO30_05570"
     CDS             complement(2288..3040)
                     /locus_tag="VFO30_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418791.1"
                     /translation="MVQVITQKSAPSTADKPIKAITKKRTPATARKPTQPVKQEPAQA
                     IAKEPTQAMTKKPASAITQKPSQAIAKESGRTFAIEPVRPMGQQSVSHTTQPFSRFSF
                     KTPGGKKESVPIITQYYPNQIVYPYGKIDRHIDGRLMQAATIAQERAHAHSRSRCWHY
                     VKDALLASGVIDSRPKTELARDAASELVSNYGFKRLPVSDPFSAPVGSVLVYGTARSV
                     GHVELRTRDGFVSDFRSKTPSRRPLLGVYAKL"
BASE COUNT          788 a         1102 c         1160 g          881 t
ORIGIN      
        1 aagctggtcg cagcggcgtg tcagccgggc aagaagctgg ttcacccgga actttttctc
       61 gttcgtccat tggcagacgg cattcaatat tcgccgtcgt cgtcgttgca gatacacgct
      121 cgctttaatt cccgccggcg cgccgaacag ctgccgcaaa tcggcgtcgt agaaatccgg
      181 atacgtatcc tcgtaaagct tgcgtttgcg cgcgtagtag gtcttgagtt tgacattcaa
      241 gcagtcgtaa tcagctacgc gatactcagg catgtggacc ggtggcttcc cggctaacga
      301 gcgcattagc tcatcgacat attccagttt ttgaagtgcc ctccagcgcg agtaacgctt
      361 gcgccaatcc aggccgggcg tcagccagac agcgaaagtc tccgcgaaat cttcatccgg
      421 atgactctgc gcgtaccagt cttctagatg catcacataa ccgcggctga aaggtcgcgg
      481 ccggtaagtg tctggcgttt cctcgcgcga agtttgccca aagagctcct gccatttctt
      541 cttccgagta agtcggtacg cgtacatata agcgtgaccc gcttcatgtc gcatcaattt
      601 catgaaccac tcgggcgtcc ctccttcgac ctcaagcatc atcgtccgct ccaacgcccg
      661 caaccggtcg tgaacaagga aaaaaggaat aaaaattgcc ggtatgccga ttggtacaaa
      721 ccactcatcg ccgacgtgac acggcggatg aaacgtgagt ccctttgccg atagctcgtc
      781 gtaaagttgc cggattagcg gttcaagcgg cgtgccctcg agccgcaaac cgagcgcgga
      841 aatgcggcgt tggagaagtt cctggtcgct gagcgatggc caatcggccg cttccatttc
      901 cggcatggta tcagtcccaa cggacgtggc aaccgtcacc ccgttcgttc ggcgcgaagc
      961 gcgtcttcat tgagtgctgg aggttgaacg cgtgcggtgc cgtggtcttg cgaaggcggg
     1021 ttgggcattg aacctttgcg ttcatgcacg acatctttcg aatccgtcgc gctacggtgc
     1081 aagatgccga cgtcatcgcg tggcatcgtg cgcggatgtt tcaagacatg ggcgacgttt
     1141 ccgatgacgg ttttgaaatt ttgcgcgcga aagcgcgttt gcggctaaag gaatggctcg
     1201 agagcggcga ctacatcggc tggctcgcaa ctcctgccga taaaccggaa acggtcgttg
     1261 gcggtgccgg cattcagttg caaccgatcc tgccccgccc gcgcgatgca tcgacgattg
     1321 gcgaaggccg gcagggaacg attgtgaacg tctttaccga accgcaatgg cgacggcgcg
     1381 gggtcgccgg cgggctcctt aaagagatca tcgcttggtc gaagaacgaa ggtctcgacc
     1441 ggcttttact tcacgcgtcg gacgacgggc gctcagttta cgaacggctc ggattcatcg
     1501 ccggcaacga aatgtatttc gccggcgttt cttagttctt ccgcccgacg atgcccaatc
     1561 ggttttggag cgttgccgag acgagcttcg ttgtcgcttg ggtcggcgac gcatgatgtg
     1621 accaattgac aagtgtggcc aacacgtcta aagggaggcc gactcgtcac accgagtcgt
     1681 tgtcaaaagt ccgcgcgtcc cccaaggggc tagcgcgccg ggctgttccg aatccgaagt
     1741 taaaagctgc attcctgcgg ctttcggtat cgagactggc ttggtccacc cgagactcag
     1801 ccacgttgaa acattatgaa gctattttgt acagtcatat ttttctcatc gttggcatcg
     1861 atcgctttcg cgcgcgagga gtcggtcctt gcacgcgtta ccagttactg ggcaggcgag
     1921 ggccataaat acgccagtac cggccaacga ttacgctcgg gacattgcgc cgtcgatccg
     1981 aaacgaattc cgtacggcag caagattgtc ttccctgatc gcgcctgcac tgcagtcgac
     2041 accggacctg cggtcattag ccgcaaagcc gctcgtctat gcggacggac tgcgagccaa
     2101 ctcaaggcga ttgtggtcga ccgatttttt gaaacaaagc aagaagcaat ggcgtggacg
     2161 agtgcgcatc cacactttat gacgctccaa attgttcggc ccggatctca cgcagaacca
     2221 tccgacctgt gatcagatag gaaaacgagt ggatcgcttc tcccagcacg agaagtcccc
     2281 gagaaactta caacttggcg taaacgccca acaaaggacg gcgcgatggc gttttcgaac
     2341 ggaaatcgct aacaaaaccg tcccgcgtgc ggagctcgac atggccgacg ctgcgcgcgg
     2401 taccatacac tagcactgaa ccgaccggcg cggagaacgg atctgacact ggaagtctct
     2461 tgaatccata gttactgacg agctctgatg cagcgtccct ggcgagctcg gttttcgggc
     2521 gggaatcaat aacaccggaa gcaagcaatg cgtcttttac gtaatgccag catctcgagc
     2581 gcgaatgcgc atgggcgcgt tcctgcgcga tggtggcagc ttgcatcaat ctgccgtcga
     2641 tgtgccgatc gatcttgcca tacgggtaaa cgatctggtt gggataatac tgcgtgatga
     2701 tcggaacgga ttcctttttg ccccccgggg tcttgaagct aaaccgcgaa aagggttgtg
     2761 ttgtatggct tacggattgt tggcccattg gtcggactgg ctctatcgcg aacgttcgcc
     2821 ccgattcttt ggcgattgcc tgcgacggct tttgcgtgat cgcactcgcc ggctttttcg
     2881 tcatcgcttg cgtcggttcc ttggcgattg cttgcgctgg ctcttgtttg accggttgtg
     2941 tcggcttcct cgcggttgcg ggcgttcgct ttttcgtgat cgcttttatc ggcttatccg
     3001 cggtactcgg tgccgacttc tgcgtgatca cctgaaccat gtcgtccgct tttttttgat
     3061 cgtggctcgc aagatccaac gacgaaggag ccaacgcctg cgcgcgaagc gctgcgctgg
     3121 aaatgacaag aagcgatgca gttgcgaatg cccggagggc aggtcgtttc cccacgaagg
     3181 cgaggttatg ccgggaatcg agccgctagg caagcttttt tttcttattt tttcgacagg
     3241 ttggaaggcc aaaatctgac ctgttcggaa ttacgagccg ttgtgtctcc gcgcctcgcg
     3301 cgcgtgccct cctgaggagc taattgtaag gaaaataatc cggcccaaat tgtggcggag
     3361 gagcaaacat ttgatcccgc tggatgacag cgcggcgatg tctgcgcgga acgaattggt
     3421 tttcgtccga gtcgggcgcg acgatcctgg tgcggcccga aggcaaacga taaatcagcc
     3481 gtccatcgga cgtgattcca agcattcgga cgcgcatcga accctggcga ggcgacgatg
     3541 tagaagcaac gcgtttcttt ttgctccgcg agctcgactg acccgtagta gccggctgtg
     3601 caacctcgga gcttatcggg ggtgccgtag cagcctcgct cgcagctgag gtttccggaa
     3661 ccgcgcccgg ggaagcggcc gccgcgaaag cgtccggagt tgcggccacc tgcggctgct
     3721 cgctcgatgt ttggccctgc tggagatccg ccgctggggt ctgagacgaa tctggggctg
     3781 ccgcgccgtc cgttgctggt tggttcgcta gcgccagcgt tgcatttgcg gcttgcgaca
     3841 gcacagtcgc aggagcgacg ggcgtcgaag cattttggac tgccgcaggg gttggtgaag
     3901 attcggggac gccaaccaga acgccgattg a
//
LOCUS       DASTDG010000170         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_67508, whole genome shotgun sequence.
ACCESSION   DASTDG010000170 DASTDG010000000
VERSION     DASTDG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67508"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFO30_05575"
     CDS             <1..448
                     /locus_tag="VFO30_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9418792.1"
                     /translation="FIERLPDQLESIVGERGVKLSVGEKQRLSIARALLKDPPILVLD
                     EATASVDTATERLIQEALERLMSNRTSIVIAHRLSTVVHADQILVLDHGRIIERGRHD
                     ELIALGGKYARLCKQSLLETSPRPEAEEPVDLLAAEMATPEERLSI"
     gene            complement(530..1000)
                     /locus_tag="VFO30_05580"
     CDS             complement(530..1000)
                     /locus_tag="VFO30_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9418793.1"
                     /translation="MKWVAMGALLSVGLVSPISAQTPASPTPSPSDAAPLEALVKKID
                     EQNAKIDALSQQILKLQQEIANKRPGVMIGETPEATSTSAPGETAERPVGANTHTVVR
                     GETLTSIAKQYKVSIEDLQRVNHIEDGRRLQAGQTITIPSPPVQPPPPSPAPSP"
     gene            1088..1264
                     /locus_tag="VFO30_05585"
     CDS             1088..1264
                     /locus_tag="VFO30_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HET9418794.1"
                     /translation="MPDRIQKPAKPVPWGPKPGDGGGPRSPDVSRPDTKDLLKKMRKV
                     DPNQSKRYRQRTGQ"
     gene            1264..2097
                     /locus_tag="VFO30_05590"
     CDS             1264..2097
                     /locus_tag="VFO30_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET9418795.1"
                     /translation="MDPRTVTLDQPFAGASEDFPALLRRKSLLAPGGIVEQTSRTDKS
                     QHDSFTPTQATTILAFKFSGGVLVAGDRRATAGNTVVYDRADKVLEIDRHSIMAIAGV
                     PATAWEMARVLEHSFQYFRRTQLQEMSVDGKVRALSKLLRDNFGFVMQGVGMVVPIFA
                     TFDFNHANPGPRLYFYDAMGAQFEVADYAATGSGSPAVRGILYYENNWGAKTLQKLNV
                     DEAVSIALRALDTAAESDTATGGVDRSGKIYPLIKIVSRDGITTLLEEKIAAVFEQTV
                     A"
     gene            2094..2873
                     /locus_tag="VFO30_05595"
     CDS             2094..2873
                     /locus_tag="VFO30_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418796.1"
                     /translation="MIEEPYRWVEAIANRREYIETQLATGSPIAAAGYHDGILFVTIG
                     KNRQKLFEIYDRIAMGVIGHPGDIERLRMGAIEMASTEGFTRSAADVSLRRLAHYSLS
                     PVMKTAFEQVYGAPYLARLLFAEIGATPEDDLFLRVDYDGSIATNGATVAQARQGFAV
                     LSGTRTGTELMESFLRDEHAPAAALEQALATALDAWSIGHLAAQQPDMNSAPERAAIT
                     QHRQEQLAEAAIEAAILERNAINAIRYRALSDKEVRELVRG"
     gene            2929..3690
                     /locus_tag="VFO30_05600"
     CDS             2929..3690
                     /locus_tag="VFO30_05600"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9418797.1"
                     /translation="MAWLGLIVMIASAAWWERTATPSTPRPSEVASVPETASVVVIDP
                     GHGGQDSGAMCAGMLEKDLTLDIGQRVDRLLQAQGVSTVLTRVGDAYVSLPDRAALTN
                     RVPHCVLVSIHFNEDNKPVSSGVETYYAEHQTPPVPALASWLPFWQKAVATEPNLESQ
                     SLAGFIQQELVARTQALNRGTKPQQFFVIANVRHPAVLVEGGFLTNKEDVAKIVNPAY
                     REQIAVAITEGILRYRELAKNRAATLAVSASGRAE"
BASE COUNT          778 a         1179 c         1182 g          767 t
ORIGIN      
        1 attcatcgag cgcttgccgg atcaactcga gagcatcgtc ggcgagcgcg gcgtgaagtt
       61 aagcgtgggc gaaaagcagc gactttccat cgcgcgggcg ctgctcaagg acccgccaat
      121 tctggtgctg gatgaagcga cggcgagcgt cgacaccgcg acggagcgac tgatccagga
      181 agcgctggag cgcctgatgt cgaaccgcac ttccattgtg atcgcgcacc ggctttcgac
      241 cgtggtccac gcggaccaga ttctcgtgct cgatcatggt cgcatcatcg agcgcggccg
      301 gcacgatgaa ctaattgcgc tcggcggaaa atacgcgcgc ttgtgcaagc aaagcttgct
      361 cgaaacttcg ccgcggcccg aagcggaaga accggtcgat ttgctcgctg ccgaaatggc
      421 tacgccagag gagcgattgt cgatctaagt cgtcagctct tcgcctaacg agtgacctcg
      481 cggccatatc cggatccgct gctcacgctt tcatttgcag ctatttcgat tacgggctcg
      541 gggcgggcga aggaggaggc ggttgtaccg gcggtgacgg aatcgtgatc gtctgaccgg
      601 cctgcagtct tcggccgtct tcgatgtggt ttacccgttg caggtcttcg atgctgacct
      661 tgtactgctt tgcgatcgaa gtcagcgttt caccgcgcac cacggtgtgt gtgttcgccc
      721 cgacgggacg ctcggccgtt tcacctggtg cggaggttga tgtcgcctcc ggagtttcac
      781 cgatcatcac accgggtcgc ttgttcgcga tctcctgctg cagtttcaaa atctgctgcg
      841 aaagcgcgtc gatcttcgcg ttttgttcgt cgattttctt cacgagcgcc tccagcggtg
      901 cggcatcgga cgggctcggt gtgggcgagg caggcgtttg cgctgaaatc ggcgacacca
      961 accccaccga caaaagcgcg cccatcgcca cccatttcat cgcgccatgt tgccattgtg
     1021 ccttttatct ggcaagccat cgcagccgcg tagcttgcct ttcattcatt tgcccttagt
     1081 tttttgcatg cctgatcgca ttcagaagcc ggctaagccg gtgccgtggg gaccaaaacc
     1141 gggcgatggc ggcgggccgc gttcgccgga cgtgtcgcgg ccggacacaa aagatctcct
     1201 gaaaaaaatg cgaaaggtcg atccgaacca gtcgaagcgc tatcgccagc gcacgggtca
     1261 gtaatggacc cgcgcacggt cacgctcgac caaccgtttg ccggtgcgag cgaagatttc
     1321 cccgcgctct tgcggcgaaa aagtttgctc gcgccaggtg gaattgtcga gcaaaccagt
     1381 cgcaccgata aatctcagca cgattcattt acgccgacgc aggcgacgac gatccttgcg
     1441 ttcaaatttt ccggcggcgt cctggtcgca ggtgaccgtc gcgcgaccgc tggtaacacc
     1501 gtcgtctacg atcgtgccga caaagtttta gaaatagacc gacattcgat catggccatc
     1561 gctggcgtcc ccgcgacggc ctgggagatg gcacgcgtac tcgaacattc gtttcagtat
     1621 tttcgccgga cgcagcttca ggaaatgagc gtggacggaa aagtacgcgc gctttcgaaa
     1681 ttgctgcgcg acaacttcgg cttcgtcatg cagggcgtcg gcatggtcgt accgattttc
     1741 gcgacctttg atttcaacca cgcgaacccg ggcccccggc tctatttcta cgacgccatg
     1801 ggcgcgcagt tcgaggtagc cgattacgcc gcgaccggtt ccggctcgcc ggctgtgcgc
     1861 ggcatcctct attacgaaaa caactggggc gcgaagacgc tgcaaaagtt gaacgtcgac
     1921 gaagcggtat cgattgcgtt gcgcgcgctc gataccgccg ccgagtcgga cactgccacg
     1981 ggtggagtgg atcgcagcgg gaaaatttat ccgctcatca aaatcgtttc gcgcgacggc
     2041 atcacgacat tgctcgagga gaagatcgcc gccgtctttg agcaaacggt cgcatgatcg
     2101 aggagccgta tcgctgggtc gaagcgatcg cgaatcggcg cgagtacatc gagacgcagc
     2161 ttgcaaccgg cagtcccatc gccgcggccg gttatcacga tggcattcta tttgtcacga
     2221 ttggaaaaaa ccgacagaaa ttgttcgaga tttacgaccg catcgcgatg ggcgtgatcg
     2281 gtcaccccgg cgacatcgag cggctgcgca tgggcgcaat cgaaatggcc agtacagaag
     2341 gattcacccg ctcggcggcc gacgtttcct tgcggcgact ggcgcattac tcgctcagcc
     2401 cggtgatgaa aacggcgttc gaacaggtct atggcgcgcc ttatctcgca cgcttgctat
     2461 ttgctgagat cggcgcgacg cccgaagacg acctgtttct gcgcgtcgac tacgacggct
     2521 cgatcgcgac caatggcgcc accgttgcgc aggcgcgcca gggtttcgcc gtgctcagcg
     2581 gcacgcgcac tgggaccgaa ttgatggaat cgtttttgcg cgacgagcac gcgcctgctg
     2641 cggcgctgga acaggcgctg gccaccgcgc tcgatgcgtg gagcatcggc catttggcag
     2701 cgcaacagcc agatatgaat tcagcgccag aacgcgcggc gatcacgcaa catcgacaag
     2761 agcaactcgc cgaagctgcg attgaagcgg ccatcctcga gcgcaacgcc attaacgcga
     2821 ttcgttatcg cgctttgtcc gataaagagg tgcgcgagct ggttcgcggc taagacacat
     2881 ccgatgttcg ggcgagggtc tcggctgggc cgcgcgatgc gcagcggaat ggcctggctc
     2941 ggcttgatcg tgatgatcgc gtcagccgca tggtgggagc ggaccgccac accatcgaca
     3001 ccgcggccga gcgaagttgc gtcggtgccg gaaaccgcat ccgtcgtcgt aattgatccc
     3061 ggccacggtg gtcaggattc tggggcgatg tgcgccggca tgctcgagaa ggatctcacc
     3121 ctcgacatcg gccagcgcgt tgatcgcctc ctgcaggcgc aaggcgtatc cactgtgctc
     3181 acccgcgtcg gcgacgctta cgtatcgctg cccgaccgcg ctgccctgac gaatcgtgtg
     3241 ccacactgcg tgttggtcag cattcacttt aacgaggaca acaagccggt ctcgagcggc
     3301 gtcgagacct actacgccga acatcaaacg ccacctgttc cggcgctcgc atcctggctg
     3361 ccgttctggc aaaaagccgt ggccactgag ccgaacctcg agagccaaag tctcgcgggg
     3421 tttattcagc aggaactcgt cgcacgcacc caggcgctga atcgtggtac caaaccgcag
     3481 caatttttcg tgatcgcaaa tgtgcgtcac cccgcagtgc tggtcgaagg cgggtttctc
     3541 acgaacaaag aagacgtcgc taagatcgta aaccccgcgt accgggagca gatcgcggtc
     3601 gccatcacgg aaggcattct gcgctaccgg gagcttgcga agaatcgcgc cgccacgctc
     3661 gcggtcagcg cgtccggccg cgccgaataa ccccgcccgt gcagcgtcca aaccggacag
     3721 aaatcgacat gagaattagg atcgctgtcg cggcgctgat ttttggcact ggcctggttc
     3781 gcgccgacca aacaattgcc gaagtgcagc aggcgttgaa ggagcagggg ttttactatg
     3841 gcgagatcag cggcgaaaaa actgccgaca cgaccgcggc gatccgccgt tatcagattc
     3901 gcaatg
//
LOCUS       DASTDG010000171         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_440631, whole genome shotgun sequence.
ACCESSION   DASTDG010000171 DASTDG010000000
VERSION     DASTDG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440631"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFO30_05605"
     CDS             <1..1068
                     /locus_tag="VFO30_05605"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9418798.1"
                     /translation="DRQSILYTSNQNDVDRRHIWRVSATGGDPQALTSGETIEWNPVE
                     TSDGKNVLCLGSTATSPSMPYRVTSGGRELIAKNAVPPEFPEAQLVAPKQVVFKSEDG
                     LDIHGQLFVPKNQTERGPALIFTHGGPIRQMLLGWHYMQYYYNAYGMNQYLASKGYTV
                     LSVNYRLGIMYGRAFREPPNSVWRGASEYKDVVAGAKFLQTLENVGPHRIGLWGGSYG
                     GFLTAMGLARNSDLFKAGVDFHGVHDWATFLPMWTENPDAAPDVKEARDLAFKSSPVA
                     STAAWRSPVLFIHGDDDRSVPFQQTTDLVEKLHAQNVDLEELVFPDEIHDLLRRASWI
                     RAYKATAQFFDRKLSARSAGP"
     gene            complement(1509..1841)
                     /locus_tag="VFO30_05610"
     CDS             complement(1509..1841)
                     /locus_tag="VFO30_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418799.1"
                     /translation="MVRGVLGAVCAVALLIQPLGYAQPTSGRPDPKTAAEMTGTIKEY
                     TAGGSLVLETLPPSEPVQFKLSKNVTYADADGKTIEAAGLTTNQKVRIHYMKVGGDNV
                     ADEVTLIQ"
     gene            complement(1991..2251)
                     /locus_tag="VFO30_05615"
     CDS             complement(1991..2251)
                     /locus_tag="VFO30_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9418800.1"
                     /translation="MFHLIWYILVGLVAGLIAKSVMHVHMTIFWTIVVGIIGSIIGGA
                     VTHMFTRPADERYHPAGLIFSTLGAILVLFVCYKMHIRFPRV"
BASE COUNT          575 a          713 c          816 g          557 t
ORIGIN      
        1 gatcggcaga gcatccttta cacgtcgaat caaaacgatg tcgatcgccg ccacatttgg
       61 cgggtgagcg cgaccggcgg cgatccgcag gcgctgacga gtggcgaaac gatcgaatgg
      121 aacccggtcg aaacgagcga cggcaagaac gtgctctgcc tcggctcaac cgcgacatcg
      181 ccatcgatgc cttatcgcgt cacgtcgggc ggccgcgaat tgatcgcgaa gaacgcggtg
      241 ccgccagagt ttccggaggc gcagctcgtt gcgcccaagc aggtcgtgtt caaaagcgag
      301 gacgggctcg acattcacgg ccagctcttt gttccgaaaa accagacgga gcgcggaccg
      361 gcgctgattt tcacgcacgg cggtccaatc cggcagatgc tgctcggatg gcattacatg
      421 cagtattact acaacgccta cgggatgaat cagtatctcg cgagcaaagg ctacaccgtg
      481 ctttcggtga actaccggct aggcatcatg tacggccgcg catttcgcga gccgccgaac
      541 tccgtctggc gcggcgcttc ggaatacaaa gacgttgtgg cgggcgcgaa gtttttgcag
      601 actctggaga atgtcggccc gcaccggatc ggattgtggg gcggctccta cgggggattc
      661 ctcaccgcga tgggtctggc gcggaactcg gatctcttca aagctggtgt cgattttcat
      721 ggtgtgcatg attgggcgac gtttctgcca atgtggaccg agaacccgga cgcggcgccg
      781 gacgtgaagg aagcgcgcga cctcgcgttc aagtcatcgc cggtcgcgag caccgcagcg
      841 tggcgctccc cggtgctgtt cattcacggc gatgacgatc ggagtgtgcc gttccaacaa
      901 acgacggatc ttgtcgaaaa gctacacgca cagaacgttg atcttgagga actcgtgttt
      961 cccgacgaga tccacgatct cctccgccgc gcgagctgga ttcgcgccta caaagcaact
     1021 gcgcagtttt tcgaccggaa gcttagtgcg cgctcagccg ggccatagga ttgtccgccg
     1081 aagctgacga tcgtcgcggg gaaaatgagg atccgctttt gccgagccgc cttcattaag
     1141 aactacggcg tgcgatgggc tatggcgcga caggagagca aacgccaaac gccgaacgcc
     1201 caatgctgaa tgaaatagga cttcccggcc gacagtcgcc gtcgccgacg gcgatggcgt
     1261 ggcatggggc cgccgctaca gccaccgagt cagcgtttat cggcggaatg aagcaagtgc
     1321 cagagcagca ccttatcaaa cgcctgctgc cgcttaacaa ccaaacggtc tttgcgcgcg
     1381 gctgcggtga gtcgcgataa aaatgggaat ctgcgtttgg ctggtctgtg ttgcgcgaag
     1441 taaagcgcgc tgttttgcgt gatagtgggt tctagcatag gtgtgggtct tctgttttct
     1501 gaacttcgtc actgaatcaa agtcacttcg tccgcgacat tgtcgccgcc gaccttcata
     1561 tagtggatgc gcaccttctg attggtggtt aagcccgccg cttcgatcgt tttgccgtcg
     1621 gcatcggcgt aagtgacgtt cttactcaac ttaaactgaa caggctcgct cggaggcaac
     1681 gtttccagga ccagggagcc gccggcggtg tattccttga ttgtgccggt catctcggca
     1741 gcggttttcg ggtcgggacg gccgcttgtc ggctgtgcgt agccgagcgg ctgaatcagc
     1801 aaagcaacgg cgcaaacggc gccgagaacg cctctcacca tagaatttcc cttaatcccc
     1861 tatctttttt acgaggctca gggagcggct ggatgtatgg tttttgcagt cggcatgatt
     1921 atttcaagag ccgaaaagcc gaacgcccaa cctccaatac gagagaccgg atgacctgcg
     1981 tccggcccag ctagacccgc gggaagcgga tatgcatttt ataacagacg aacagaacca
     2041 ggatcgcgcc gagggttgag aaaatgaggc cggccggatg ataccgttca tcggccggac
     2101 gcgtgaacat gtgggtaacg gcgccgccga tgatcgagcc gatgatgccg accacgatcg
     2161 tccagaagat cgtcatgtgg acgtgcatga ccgatttcgc gatgagcccg gcgacgagcc
     2221 cgacaagaat gtaccagatt aagtgaaaca tagatttgct ccttgttttg cccgaagaaa
     2281 ggggccgagt atgggcgggc ggttcgtggt tggccagatt tttcgggcaa ggcttctgag
     2341 cctcgagaag gccgcgttgg cgagaaataa ataaaagtga aacgaagttg acgggtgccc
     2401 gcagcctcgt atgatttgag tcccgcgata aaacgggttc ccgaaagcat tcggggcgtt
     2461 cttgctggct cagggctgag ccgatcttgg tcggacatga cgagggtcgg gttttgccgt
     2521 gtaccggagg ggaacatgtc gatctttgac agtttggtcg cggatttcga aaggcacaca
     2581 cgacgtgcga aatcagcgat gagataagtc agcgaaaatt gaattgtgcg ttatttcgtt
     2641 attttctttg atcaaaactt t
//
LOCUS       DASTDG010000172         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_404731, whole genome shotgun sequence.
ACCESSION   DASTDG010000172 DASTDG010000000
VERSION     DASTDG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404731"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2883)
                     /locus_tag="VFO30_05620"
     CDS             complement(<1..2883)
                     /locus_tag="VFO30_05620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET9418801.1"
                     /translation="MIQLKHGLALSSLQRAAKSTHRKINRLPKKRNAGLRFERVFSDA
                     AVAPFEQIEWERRTAEITDDSGKTIFKQDNIEAPKSWSALATKIAVSKYFYGDIANGT
                     DPHNGGRETSVRQLVHRVTRTITDWGIADGYFANAEAAEIFYDELTWLCVNQHGAFNS
                     PVWFNVGLYHQYGIGKDAGAGNYFYNRATGQAERAPTQYEYPQGSACFIQSVDDTMED
                     IMRLAMSEAMLFKYGSGTGTDLSTLRSTREKLSGGGKPSGPLSFLKVYDQIANVVKSG
                     GKTRRAAKMNTLKDWHPDIEEFIEAKQKEEKKAWALIEQGYDGSYNGDAYGSVMYQNE
                     NLSVRVSDAFMNGALEGREWWTRRVTDEKPCECKDARVLLRKIAEGTHVCGDPGMQFD
                     TTIHKWHTCKGTGRQNSTNPCSEYLFLDNTACNLASLNLMKFKAADGSFDVERFKAAV
                     RIFITAQEIMVDNASYPIKEIAENSHIFRTLGLGYANLGSLVMSYGYGYDSAEGRALC
                     GAITAIMTGQAYEQSARLAGAIGPFPGYKDARCSGVPKPVAKNNVASMLEVIELHRCA
                     VNEIADVEEFAYLKTEAAKTWKRAAELGRRHGYRNAQVTVLAPTGTISFLMDCDTTGI
                     EPDIALVKYKLLAGGGMLKIVNQTLKPALEKLGYNSEEIERIIAHIDAFDTIEDVIDN
                     DGAMVSSGLKREHLSIFDCAFKPFKGERSLSYMAHLRMMAAAQPFLSGAISKTVNMPE
                     SATVNDIMDTYVEGWRLGLKSIAIYRDGSKRSAPLNTRKTKDMGALEDVDLATDVTVE
                     REDFQKRILELEQEIATLRARVDQPIRHRMTDTRMSLTHKFEIAGHEGYITVGLYENG
                     QPGELFIHMAKEGSTIGGLMDTIGTLTSVALQYGVPLESLARKFAYQRFEPSGFTKNP
                     DIRNATSITDYVFRWLACKFIKGYKEATSPNRGQPDLPIKEIAEIEK"
     gene            complement(3095..3184)
                     /locus_tag="VFO30_05625"
     tRNA            complement(3095..3184)
                     /locus_tag="VFO30_05625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3146..3148),aa:Ser,seq:cga)
BASE COUNT          595 a          947 c          906 g          781 t
ORIGIN      
        1 cttttcgatc tcggcaatct ctttgatcgg cagatcgggt tgcccgcgat tcggcgaggt
       61 cgcttccttg tagcctttga tgaatttgca tgcgagccag cggaacacgt aatcggtgat
      121 gctggtggcg ttgcggatgt ccgggttttt tgtgaatccg ctcggctcga accgctggta
      181 agcgaatttt ctcgcgagcg actcgagcgg cacgccgtat tgcagcgcga ctgaggtcaa
      241 cgtgccgatc gtgtccatga gtccgccgat agtgctgcct tccttggcca tgtgaatgaa
      301 cagttcgccc ggctggccgt tctcatagag cccgacggtg atgtagcctt cgtgtccggc
      361 gatctcgaat ttgtgcgtca atgacatccg cgtgtcagtc atgcgatgcc ggatcggttg
      421 gtcgacgcgc gcgcgcaacg tagcgatctc ctgctccagc tcgaggatgc gcttctggaa
      481 atcttcgcgt tcgactgtga cgtcagtggc aagatcgaca tcttccagcg cgcccatgtc
      541 cttggtcttg cgcgtgttca acggcgcgga gcgtttcgac ccgtcgcgat aaatcgcaat
      601 cgacttcaag ccgagccgcc agccttcaac gtaagtgtcc atgatgtcgt tgacggtcgc
      661 cgattccggc atgttcaccg tcttggagat cgcgccgctc aggaacggtt gcgccgccgc
      721 catcatccgc agatgcgcca tgtaactgag cgagcgttcg cctttgaatg gcttgaacgc
      781 gcaatcgaag atcgacagat gctctcgctt taagccggac gaaaccatcg cgccgtcgtt
      841 gtcgattacg tcctcgatcg tgtcgaatgc gtcgatgtgg gcgatgatcc gctcgatctc
      901 ctctgagttg tagccgagtt tttcgagcgc cggtttgagc gtttggttga cgatcttcaa
      961 catgcccccg ccggcgagca gcttgtattt caccagcgca atgtccggct cgatcccagt
     1021 cgtgtcgcaa tccatcagga aactgatcgt gccggtcggc gcgagcacgg tgacctgggc
     1081 gttacgataa ccgtgccgcc ggccgagctc ggccgcgcgt ttccatgtct tcgccgcctc
     1141 ggtcttcaag tatgcgaact cttcgacgtc agcgatttca tttacggcgc atcgatgcag
     1201 ctcgatgact tcaagcatcg acgcgacgtt gttcttggcg acgggtttcg gcacgccgct
     1261 gcaacgcgcg tctttgtagc cggggaacgg gccaatcgct ccggccaatc gcgcgctttg
     1321 ttcgtaggcc tggccggtca taatggcggt gatcgcgcca caaagcgcgc ggccttccgc
     1381 gctatcatag ccgtatccgt agctcatcac caacgagccg agattggcgt aaccgaggcc
     1441 tagcgtgcga aagatgtgcg agttttcggc gatctccttg atcggataac tcgcgttatc
     1501 gaccatgatc tcctgtgcgg tgatgaagat gcgcaccgcc gccttgaaac gctcgacgtc
     1561 gaagctgccg tcggccgctt tgaacttcat cagattaagc gacgcgagat tgcaggccgt
     1621 gttgtcgagg aacagatact cgctgcacgg attggtggaa ttttgtcgtc cagtgccttt
     1681 gcaggtgtgc catttgtgaa tcgtcgtgtc gaactgcatc ccgggatccc cgcagacgtg
     1741 tgtgccttcg gcgattttgc gcagcaacac gcgcgcgtct ttgcattcgc atggcttctc
     1801 gtcggtgacg cggcgcgtcc accattcacg accttcgagc gcgccgttca taaacgcgtc
     1861 gctgacgcgc acgctcaggt tttcattttg atacatgacc gagccgtaag catcgccgtt
     1921 gtaactgccg tcgtaacctt gctcgatcaa tgcccacgct ttcttttctt ccttctgctt
     1981 cgcctcgatg aactcctcga tgtccgggtg ccagtctttc agcgtgttca tcttcgccgc
     2041 gcggcgggtt ttaccgccgc tcttcacaac gttcgcgatc tgatcgtaaa ctttcaaaaa
     2101 ggagagcggc ccgctcggtt tgccgcctcc gctcagcttt tctcttgtcg atcttagagt
     2161 cgacaaatcc gtcccggttc cgctgccgta cttgaacaac attgcctcgc tcatcgccaa
     2221 ccgcataatg tcttccatcg tgtcatccac cgactggatg aaacaggcgc tgccctgcgg
     2281 gtattcgtac tgcgtcggtg cgcgctccgc ctgaccggtg gcgcgattgt aaaaataatt
     2341 gcccgcgccc gcgtctttgc cgatgccgta ctgatgataa agaccgacgt tgaaccagac
     2401 cggtgaattg aatgcgccat gctgattgac gcagagccac gtcagctcgt cgtaaaaaat
     2461 ctcggccgcc tccgcattcg caaaataacc gtctgcgatg ccccagtctg tgattgttcg
     2521 cgtgactcga tgcaccagtt gccgcactga agtttcccgc ccgccgttgt gcgggtcggt
     2581 cccattcgcg atgtcgccat aaaaatattt cgagaccgcg attttggtgg cgagtgcgct
     2641 ccagctcttt ggcgcttcga tgttgtcctg cttaaaaatt gtcttcccgc tgtcgtccgt
     2701 gatctcggcc gtgcgccgtt cccactcgat ttgttcgaac ggcgcgaccg cagcgtcgct
     2761 aaacacgcgc tcgaaacgca gaccggcgtt cctctttttt ggaagccgat ttattttccg
     2821 atgcgttgat ttagccgcgc gttggagcga ggagagggcg aggccatgtt tgagctggat
     2881 cataagcgga aagggcggtt gaggctgtga attgcggtta acagtcgtga acagcccggc
     2941 caataacctg tgaacggtgg cgaacaacaa gtaatagcgg agctgctcgg gcggacacac
     3001 aatatgcagc gccgaaaaca gccttggcaa gggcaaaatt gtaacatttc ttggccggtc
     3061 gaaccgcgtt cctgacctga tttgaaccag ccagcggaag gggtgggatt cgaacccacg
     3121 gtgggtttca agcccacgct cgatttcgag tcgagtgcct taaaccggac tcagccaccc
     3181 ttccttctgc tgaagaaaac ttcgaacgcc gaacgtccaa cattcagtt
//
LOCUS       DASTDG010000173        11704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1531803, whole genome shotgun sequence.
ACCESSION   DASTDG010000173 DASTDG010000000
VERSION     DASTDG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531803"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFO30_05630"
     CDS             complement(<1..1073)
                     /locus_tag="VFO30_05630"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET9418802.1"
                     /translation="MKRTLLGLAFFALLLPAARNATAAEVSIDFFYNNLSGGQWIEVA
                     DYGYGWQPDVAISESTWRPYTDGYWAYTDLGWTWISYEDFGWAAYHYGRWARLANYGW
                     VWFPGSDLDWGPAWVSWRVGGDYVGWAPLPPRGPGIVYRGEALVGPSVDIDFDIGPAY
                     YNFIDVRYIGEPVLRDRIFAPTQNVTYINQTVNVTNITYNNNTVYNYGPDYNTLSAQS
                     SRPIQRLAVERQSIDPTAAVKSGSVTKVQGDKLVIAAPPKIQKPTKRVAPPAVKTRVA
                     HAKIEKGWTGVADADQLKQKIKTENPKQIPPSTRGAVTGTAAAQASPAAPASPAATAL
                     GATSATSPVEPGRAGAAAAERGQA"
     gene            complement(1166..2683)
                     /locus_tag="VFO30_05635"
     CDS             complement(1166..2683)
                     /locus_tag="VFO30_05635"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET9418803.1"
                     /translation="MKNLAKFLLFVLLVSAGISLLYDYRLKHGGLGFGERRTPEKYTL
                     ASEPSVDTKQVTSLEVLNRERRALVSSVIPAVVAVKTSKKIAIRREYELDPFQFFFRN
                     PRPFRNPNEEALVQNSLGSGVMVTNEGHIITNSHVVDQVDEIEVQLSDGRTEKARLVG
                     ADAEVDLAVLKIDNPGVKPLKLGDSDAVQAGDFVLAIGNPFGFEETVTDGIISSKGRP
                     SRSDMFGDLLQTNAAINPGNSGGPLINLRGEVIGINTAIISRSGGSQGIGFAIPSNTV
                     RTALESLLKKGRIIRGYLGIRTRALQPEQTGADAEGVIVDDVLPGSPAAQAQLQKGDI
                     IRKFDRRDVKSFSALRSLVAQTELNKTIDLEIVRNGKPMTVRTQIKEEPLNYQTARVL
                     PLPQQGATPPSGAGEEEQGRGGLAAIHVGELTPELMRQLDLPQNVRGVVVTDVDPDSG
                     VAELQKGDAIEEINQQPVASVADYNNILRSLDPSQPQVLSVCRHRMRSFVVLRPQ"
     gene            complement(2683..5370)
                     /locus_tag="VFO30_05640"
     CDS             complement(2683..5370)
                     /locus_tag="VFO30_05640"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9418804.1"
                     /translation="MEISQPLTDIAWIPGPRLLALRRLGIGTVEDLLTHFPRRYEDRQ
                     QFPHFPREESDMPICLCGEVVKTSLRRFGGWKKIFEATLQESNANALSQPLVCRWFNL
                     HYIQKMIATGQRLVVFGRPKLRGNRICIDHPEFEVVEEDEETSIHFRRITPIYPATEG
                     LSQRVLRSIIYRLLAELRDAPFEPLLARELATGAEQDALRTIHFPNDWNALNAAREQL
                     VLAEFFAMQMLIASRRADSAARVGAAHCGRGELLNRFLAGLPFELTAAQHKVIAEIQH
                     DLGAHRAMNRLLQGDVGSGKTVVAIAAMLLVVEAGYQCAFMAPTQILAEQHYAVLRRW
                     LEPLGVRIAVRTGARQEESAPLPLFASASGKALGSTGCQPVTSGSLPDAVAEEVSYHR
                     RNLPHFERPWAKYAITFATRNHRQLSAEARDIVLESLLQWKDRRYELYAACVMPDHVH
                     MLIEPMVEKQSEDGKTVFFPVSKILHSIKSFTANRINKIDNASRAVWESESFDRFIRS
                     ESDLQEKFSYITRNPWDAGVVQQGHDYRWVWYPGAENAKDVAASCRDQQAGSLCSPEP
                     WISRPAAPDVIVGTHALLYENVEFSNLGLVVIDEQHKFGVAQRARLTSREPAPDVLVM
                     TATPIPRTLTMTVYGDLDVSIIDEMPRNRGKVITLLRDMSKLGEVIAFLRTQLESGRQ
                     VYIVYPLVEESENIDIKAAAAEYESWRERLHPHRCELIHGRVPAPEKQEIMERFRRNE
                     TQALIATTVIEVGVDVPNATVMLIENAERFGLAQLHQLRGRIGRGEHKSYCILLGTEQ
                     SQAASAKLQVLEKTANGFEVAEDDWNLRGPGDLLGTAQSGLPALKIGDLKRDAPLMRR
                     ARAAAMSIFEHDPCLELLENQRFRQLIVEQEGRTFSNVS"
     gene            complement(5370..7127)
                     /gene="recJ"
                     /locus_tag="VFO30_05645"
     CDS             complement(5370..7127)
                     /gene="recJ"
                     /locus_tag="VFO30_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9418805.1"
                     /translation="MQSRVGEKPIDVKPPGTFTRQSRWILAPREELSGSAIPWPELCG
                     CECIARLLMRKGFGSAEEVRDFLRPRLKSLSDPFLLPNMEAAVARVLAALNRRERIVL
                     FGDYDVDGVTSLALLAETLRAYGAAPDLFLPLRMEEGYGLSRESIERCLAQYRPQLLI
                     ALDCGSSSAEEIVDLSRRGVDVIVLDHHEPKSSLPSCVAVVNPKADAESPFHYLCSVG
                     IVFKLCHALLKRRPLDFDLKSKLDLVALGTVADIVALRGENRTLVHRGATEIAKSTRP
                     GLRKLIEVSGVKPPILPEDIGFRLGPRLNAAGRLTTAEKALRLLLTTDEAEAAELAGF
                     LDTQNRERQEVEKAILAAAEEKIATEFDGSRDAAIVVGARDWHPGVLGIVASRISRKY
                     HRPTIVIGFDAEEVGKGSGRSIEGFNLVEALCRCADKIDKYGGHEMAAGLTIQQKNLD
                     LFRDAFRAVARELLSNDDLQPRLGLDHELTFAELNYDFLHWHEMLQPFGSGNPQPLFF
                     ARKVEPASAPRVVGEKHLALRLRQRNYHHRAVYFDGAATSLPAAPWDVAFSIRAGEYE
                     GEERLEMQIHALRETASID"
     gene            complement(7136..7417)
                     /locus_tag="VFO30_05650"
     CDS             complement(7136..7417)
                     /locus_tag="VFO30_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418806.1"
                     /translation="MGRKYASALALTFVLALIGGTSQGGLHGVKIGMYDANAAATMNK
                     GTQLLKKSDYEGAAGIILPRFIETRKHGPASRAFAPHSLDDCCQKLRCD"
     gene            7598..8389
                     /locus_tag="VFO30_05655"
     CDS             7598..8389
                     /locus_tag="VFO30_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9418807.1"
                     /translation="MLDHDDLKWIASTSTDLNSLLQQISRYADLARRHKEQPDYLDLL
                     AERVELATKASQELFDRVTSKILAKTVAQSSEMAENTPPAFSVVPPPTAPAVPHEQKP
                     QSNGSKRLSEAKAPTRSSAPDLSTELEVKNPRGNREYILLVEDETQVAEVAAEMLAEE
                     GYKVIVAHDGFEALKIYRNVGKQIGLVILDYFLPVMDGDAVFEELRALNSHVNVVLSS
                     GFAEQQKISAMLAQGLRGFIPKPYSSEKLLGQVRSTLDAARQRLA"
     gene            8491..9573
                     /locus_tag="VFO30_05660"
     CDS             8491..9573
                     /locus_tag="VFO30_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9418808.1"
                     /translation="MSRIDAFFKLMAEQKASDLHLATNNPPMLRINGDLVRVDFPPLK
                     NDELKTMVYEIAPEGKIKIFEETSDVDFGYEVPGVARYRANFFQQKHGISAAFRLIPS
                     NVLTVDELGLPPVLKKFPMLKKGLVLVTGPTGSGKSTTLAAMIDYANLHRQDHIITVE
                     DPIEFVHRSQNCLVQHREVGVHTRSFAAALRGALREDPDIVLVGEMRDLETIELALTA
                     AATGHLVFGTLHTSNAAKAVDRVIDVFPTHQQNQIRTTLAESLKGVIAQNLFKRIDHP
                     GRVAALEVLVVDMAIANLIREGKTHQIPGMIQVGKKKGNQPLDDAIMDLLRKAIISPE
                     EAYDKCIDKKKFRQFLKEPPVDGSEE"
     gene            9575..10741
                     /locus_tag="VFO30_05665"
     CDS             9575..10741
                     /locus_tag="VFO30_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9418809.1"
                     /translation="MRTPDLDKILSAMLNTYDGISDLNFSVGHPLQVEDFGELKSVFV
                     DPPIEALTPYQTEQVALTLMQGNRRLIYDYLTGGSCDCSYALHDQARFRVNIFRQQGH
                     FSVVLRKLQGEVPKIESLQLPPIFHEIAKEKTGLVLVTGATGSGKTTTLAALINEINH
                     TQPVHIVTLEDPIEYVHPKAKATINQRELGHDFNNYPNGLRAALRQAPKVILVGEMRD
                     RATVEIALMAAETGHLVLSTLHTVDAGQSINRILGLFSIGEEKQLRIRLADVLRYVVS
                     QRLVPKVRGGRQLLTEVLGHNLRTREAVALGEGEHRSFYEIIEASSPFGWMTFDQSIL
                     SAYENDLISEETAKLFASKKGRISRGIDLIQKARGVDTELDSGLRLDLPANAFR"
     gene            10757..11224
                     /locus_tag="VFO30_05670"
     CDS             10757..11224
                     /locus_tag="VFO30_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418810.1"
                     /translation="MEIETDPTFLDIGDNTALVCVDHQQYQKIVVPQLIDLGYKVHLG
                     LFEEDVILKLQTYNYDVVVVYENFKESTAQTNPVLRALSRCPDARRREHFVVLLSHRF
                     PTNDAMSAFVQSVDQIVNISDLGNFKPIVRRGAVQHRELYHSFNEMVKTVQSI"
     gene            11368..>11704
                     /locus_tag="VFO30_05675"
     CDS             11368..>11704
                     /locus_tag="VFO30_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418811.1"
                     /translation="MKHSSWIRFQWDLSAFPANGSPLPPRYQIAPASGHDVEQLRKLF
                     STAFTLDSTWNAAVDEVLATVQMWLERAFASRTSTCLALRHCTRIIGASVVLGEPEAD
                     NHLTPGPCIL"
BASE COUNT         2441 a         3519 c         3310 g         2434 t
ORIGIN      
        1 gcttgtccgc gctcagccgc tgctgctccg gccctgccag gctcgacggg agaagtagcg
       61 ctcgtcgcgc caagcgctgt tgccgcggga cttgcgggag cggctgggga agcttgggct
      121 gcagcggtgc ctgtcaccgc acctcgggtc gacggcggaa tctgtttcgg gttttcggtt
      181 ttaatctttt gcttcagctg gtctgcatca gcaacgcctg tccagccctt ctcgattttc
      241 gcgtgcgcaa ctctcgtctt caccgccgga ggcgccacac gcttagtcgg tttctgaatc
      301 ttcggtgggg ctgcaatgac gagtttgtcg ccctgcacct tggtgactga gccggacttc
      361 accgcagccg tcggatcaat actctgccgc tcaaccgcga gtcgttgaat aggtcggctc
      421 gactgcgcgc tgagcgtgtt gtagtcgggc ccgtagttgt aaacggtgtt gttgttgtag
      481 gtgatgtttg tcacgttcac cgtctgattg atgtaagtga cgttttgcgt cggagcgaag
      541 atgcggtcgc gcagaaccgg ttcaccgatg tagcgtacgt cgatgaagtt gtagtaagcc
      601 gggccgatat cgaagtcgat gtcgacgctc gggccgacga gcgcttcgcc gcggtaaacg
      661 atgccgggac cgcgcggagg caacggcgcc cagccaacgt aatcgccacc gactcgccag
      721 gagacccagg ctggtcccca atctaaatcg ctgccgggga accaaaccca gccgtaatta
      781 gccaggcgcg cccagcggcc gtagtggtag gcggcccaac cgaaatcttc ataggagatc
      841 caggtccagc cgagatcggt gtatgcccaa tagccatcgg tgtagggacg ccaggtcgac
      901 tcgctgattg ccacatcggg ttgccagcca tagccgtagt cggcgacttc aatccactgg
      961 cccccgctga ggttgttgta gaaaaagtcg atggaaactt cggctgccgt cgcgttacgt
     1021 gcggccggca ataacaacgc gaaaaatgcc aaaccaagca gtgttcgttt catatcgttt
     1081 gtggatttga gtcgttagac atccgcgccg cgcggattat tcacgagcaa tgcgcagccg
     1141 ccgcctcact gcactgccgt gctaattact gcgggcgcag caccacaaat gaccgcatgc
     1201 ggtgccggca aacagacaag acctgcggct gcgatggatc aagcgaacgg agaatgttgt
     1261 tatagtcggc gacggacgcg actggctgtt ggttgatctc ttcgatcgcg tcgcctttct
     1321 gtaattcggc gacgccgctg tccggatcga catcagtgac aacaacacca cgcacatttt
     1381 gcggcaggtc gagctgacgc atcagctccg gtgtgagctc gccgacgtga atggctgcca
     1441 ggccgccgcg tccctgttct tcctcccccg cgccgctcgg cggtgtcgcc ccttgctgcg
     1501 gtaacggtaa cacgcgcgca gtctgataat tcaacggctc ctctttgatc tgggtccgca
     1561 ctgtcatcgg tttgccgttg cggacgattt ccaaatcgat ggttttgttc aactccgttt
     1621 gcgcgacaag gctgcgcagt gccgagaagc tcttcacgtc gcgccggtcg aacttgcgga
     1681 tgatatcgcc cttctgcagt tgcgcctgcg cggcgggcga gccgggcagc acatcatcga
     1741 cgatgacgcc ttcggcatca gcacctgttt gctccggctg cagagcacgt gtgcggatgc
     1801 cgaggtaacc gcgaatgatc cggccctttt tcaataagct ctccaacgcg gtgcggacgg
     1861 tgttggatgg aatcgcgaac ccgatacctt gtgatccgcc gctgcgggag atgatggcgg
     1921 tgttgatgcc gatgacttct ccgcgcaggt tgatcagcgg tccgccgctg tttccggggt
     1981 tgatcgcggc gttagtctgc agcaaatcgc caaacatatc gctgcgactc ggacggccct
     2041 tcgagctgat aatgccgtcg gttaccgtct cctcgaagcc gaatggattg ccaatcgcga
     2101 gcacaaaatc tccggcctgc accgcgtcgg agtcccccag tttcagcggt ttaacacccg
     2161 gattgtcgat cttgagcacc gcgagatcga cctcggcatc ggcaccgacc aaacgcgctt
     2221 tttcggtccg tccgtcgctc aactggacct cgatctcgtc aacttggtcg acgacatgac
     2281 tgtttgtgat gatgtggccc tcgttcgtca ccattacgcc ggagcctaat gaattctgga
     2341 cgagcgcttc ttcgttcgga ttacggaacg gacgcgggtt acggaagaaa aattgaaacg
     2401 gatcgagctc atactcgcgg cgaatcgcaa tcttcttcga cgtcttaacc gcgacaaccg
     2461 ctgggatgac gctgctcacg agcgcgcgcc gttcgcggtt gagcacctcg agtgaggtaa
     2521 cttgctttgt gtccacgctc ggctcggatg caagcgtgta tttttccggt gtccggcgct
     2581 ctccgaaacc gaggccgcca tgcttgagcc ggtaatcgta aagtaacgag atgccggcgc
     2641 tgacgagcag gacgaacaac aaaaattttg ccaggttttt catcaactaa cgttcgaaaa
     2701 ggtgcgaccc tcttgttcga caatcaactg gcggaaacgt tgattctcaa gcagctcgag
     2761 gcacgggtcg tgttcgaaga tcgacatcgc cgccgcccgg gcacggcgca tcagcggcgc
     2821 gtcgcgcttc aggtcaccga ttttaagcgc gggcaaaccg ctctgcgcag taccgagcaa
     2881 atcgcccggc ccgcgcaagt tccagtcgtc ttccgcgact tcgaaaccgt tcgcggtttt
     2941 ctccaaaact tggagcttgg ccgacgcggc ttgcgactgc tcggtcccga gcaaaatgca
     3001 gtacgacttg tgttcgcccc gaccgatgcg tccacgcaac tgatgcaact gcgcgaggcc
     3061 gaagcgttcc gcgttctcaa tcagcatcac agtggcgttc ggcacatcta cgcctacttc
     3121 gatgaccgtg gtcgcaatca gcgcctgggt ttcgttgcgc cggaaacgct ccatgatttc
     3181 ctgtttctcc ggcgctggca cacggccgtg aatcagctcg cagcggtgtg gatgcaagcg
     3241 ttcacgccat gattcgtact ccgcggccgc tgctttgatg tcgatgttct cgctctcctc
     3301 cacgagcgga taaacgatat agacctgtcg gcccgactcg agctgcgtgc gcagaaaggc
     3361 gatcacctcg cccagctttg acatgtcgcg caggagggtg atgaccttgc cgcggttgcg
     3421 cggcatttcg tcgatgatcg acacgtctaa atcgccgtaa acagtcatgg ttagggtgcg
     3481 tggaatgggc gttgccgtca tcacaagcac gtccggcgca ggctcacgac tggttaggcg
     3541 cgcgcgctgc gcgacaccga atttgtgctg ctcatctatg accacgaggc cgagattgga
     3601 gaactcgaca ttctcgtaga gaagcgcatg cgtcccgacg atcacgtccg gggctgcggg
     3661 ccgcgaaatc catggttctg gggagcacag gctgccagcc tgttggtcgc ggcagcttgc
     3721 cgcgacatct ttcgcgtttt cagcgccagg ataccaaacc caccggtaat catgaccctg
     3781 ctgcaccact ccagcgtccc aagggttgcg cgtgatgtaa gaaaatttct cctgtaaatc
     3841 gctttccgat cgaatgaatc gatcaaacga ttcgctttcc cacactgccc tgctcgcatt
     3901 gtcgatcttg ttaattcgat ttgcagtaaa ggatttgatg ctgtggagaa tctttgaaac
     3961 tggaaagaaa accgtttttc catcctcact ctgtttttcc accattggtt caatcagcat
     4021 gtggacatgg tcgggcatga cacacgccgc gtacaattca taacgtcgat ctttccactg
     4081 gagcaacgat tccaaaacga tgtcacgcgc ctctgccgat aattgtcgat gattccgtgt
     4141 cgcgaacgta atcgcgtact ttgcccacgg ccgttcaaag tggggcaggt tacggcggtg
     4201 atatgatacc tcttcggcga cagcatccgg caggctgccg gatgtgacag gctggcagcc
     4261 tgtgctcccc agagctttac cgctcgccga tgcaaacagc gggagcggcg cgctttcctc
     4321 ctgacgcgca ccggtccgca cggcgatccg caccccgagc ggctcgagcc agcgccggag
     4381 cacggcatag tgttgctcgg ccaaaatttg ggtcggtgcc ataaacgcgc actgataccc
     4441 agcttcgacg acgagcagca tggccgcgat cgcaacgacc gttttgcccg agccgacatc
     4501 gccttggaga aggcggttca tcgcgcggtg cgcgccgagg tcgtgctgaa tctcagcgat
     4561 gactttgtgt tgcgcagcgg tgagctcaaa tggtaagccg gccagaaatc tgttcagtaa
     4621 ctcgccacgg ccgcaatgcg ccgcgcccac gcgagccgct gagtcggcgc gccgggaagc
     4681 aatcagcatt tgcatggcaa aaaattcggc gagcacaagc tgctcgcgcg cagcattcag
     4741 cgcgttccaa tcatttggaa aatgaattgt ccggagcgca tcctgctctg cgccagtggc
     4801 aagctcgcgg gcgagcaacg gttcgaacgg cgcgtctctt aactcagcga gcagccgata
     4861 aataatgctg cgcagcacgc gttgcgacag accttcagtc gccggataaa tcggcgtaat
     4921 ccgccggaaa tggatggaag tctcttcatc ctcttcgaca acttcgaatt ccggatgatc
     4981 gatgcaaatg cggttgccgc gcagcttcgg tcggccgaaa acaacgagcc gctgcccggt
     5041 tgcgatcatc ttttgaatgt agtggagatt gaaccagcga cagacgagcg gctgactcag
     5101 cgcgttcgcg tttgattcct gcagtgtcgc ttcgaagatt tttttccaac cgccgaaccg
     5161 gcgcagcgac gtcttaacga cctcgccgca caagcagatc ggcatgtcgc tttcctcacg
     5221 cgggaaatgc gggaattgtt gtcggtcttc gtagcgccgc ggaaaatgcg tcagcaaatc
     5281 ctcgaccgtt ccgatgccca gccggcgcaa cgcgagcaag cgcggcccgg gtatccaagc
     5341 gatgtcagtc agcggctgcg aaatttccat cagtcaatcg acgccgtctc gcgcaaggca
     5401 tggatttgca tctccagccg ctcttcgcct tcatattcac ccgcgcgaat gctgaaggcg
     5461 acgtcccagg gcgccgccgg taacgatgtg gctgcgccat cgaaatagac cgcgcgatga
     5521 tgataatttc gctggcgcag acgcagagcg agatgttttt cgccgacgac gcgcggcgcg
     5581 ctcgccggct caactttgcg cgcaaagaac agcggctgcg gatttccgct accgaacggc
     5641 tgaagcattt cgtgccaatg gagaaagtcg tagttcagct cggcgaacgt gagctcgtga
     5701 tcgagcccaa ggcggggctg caaatcatcg ttggacaaca gttcgcgcgc gacagctcgg
     5761 aacgcgtcgc ggaataggtc aagattcttc tgctgaatcg ttaggccggc ggccatctcg
     5821 tgcccgccgt acttgtcgat cttgtcggcg catcggcaca acgcctccac gaggttgaag
     5881 ccctctatgc tgcgtccgct gcctttgccg acctcctcgg catcgaagcc gatgacgatc
     5941 gtcggtcggt ggtatttgcg cgaaatccgc gacgcgacga ttcccaaaac gcccggatgc
     6001 caatcgcgcg cgccgacaac gattgcggca tcgcgtgagc cgtcgaattc ggttgcaatc
     6061 ttttcttccg cggcagcaag gatcgccttc tccacttcct ggcgctcgcg attttgggtg
     6121 tcgagaaacc cggccaactc agcggcttca gcttcgtcgg tcgttagcag cagccgcaac
     6181 gccttttctg ctgtggtcaa tcggcccgcc gcgtttagcc gcggccccaa acgaaagcca
     6241 atgtcctcag gtaagattgg cggtttgact ccgctcactt cgatcaactt gcgtaaaccg
     6301 ggtctcgttg attttgcgat ctccgtggca ccgcggtgaa caagtgtccg gttctcgccg
     6361 cgcagcgcca cgatgtccgc caccgtcccg agtgcgacga ggtcgagctt cgatttcaga
     6421 tcgaaatcga gcggccgccg tttgagcaaa gcgtgacaaa gtttgaacac aatcccgaca
     6481 ctgcagagat aatgaaatgg cgactcggca tctgcctttg ggttcacgac cgcgacgcag
     6541 cttgggagcg aggacttcgg ttcgtgatga tcgagcacaa tcacgtccac tccgcgccgg
     6601 ctaagatcga caatctcctc tgctgacgag ctgccgcagt ccagcgcgat caaaagctgc
     6661 gggcgatatt gcgcgaggca acgctcaatg ctctcccggc tcaagccgta accttcttcc
     6721 atccgcagcg gtagaaataa gtcgggtgcg gcgccgtagg cgcgcaatgt ctcggccaat
     6781 agtgcaagcg acgtcacccc gtcgacatcg tagtcgccga acagaacgat gcgctcacga
     6841 cggttgagtg cggcgagcac gcgcgcgacc gcggcttcca tgttcggcag cagaaaagga
     6901 tcgctgagcg attttagccg tggccgcaag aaatcccgga cctcttcagc ggagccgaaa
     6961 cccttgcgca tgagcaggcg ggcgatgcac tcgcagccgc aaagttcagg ccacggaatt
     7021 gcgcttccgc tcagttcctc acgcggcgca agaatccacc ggctttgccg cgtaaaggtt
     7081 ccaggcggct ttacatcgat cggtttttcg cctacgcggc tttgcaccgc ttactttaat
     7141 cgcagcggag tttctgacaa caatcgtcta gcgagtgtgg cgcaaaggct cgtgacgctg
     7201 gtccatgctt tcgggtctcg ataaatcgcg gcaggataat accggcggcg ccttcatagt
     7261 cgcttttctt gagcagttgc gttcccttgt tcatcgtcgc ggcagcgttc gcatcgtaca
     7321 tgccgatctt caccccatgg aggccgccct gactagtgcc accgatgagc gcgagaacga
     7381 atgtgagcgc gagagcggat gcatatttcc ttcccaccgt tgcgatctta tttcctcggc
     7441 ccgcagcgcc aaggcgaaag atcacggcac gaaccgtgct caaccaaagc tatttagctg
     7501 acgtcaatga aggatttgct gccaaaccca agtccctgga ctcagcggtc gttcgctggc
     7561 gcggcacggc gtcgttagtt gaaaggcggt gggagttatg ctcgatcacg atgacctaaa
     7621 gtggatcgcg agcacgtcga ccgatctcaa ttctctactg caacaaatct cccgctatgc
     7681 cgacttggcg cggcggcaca aagagcagcc ggattacctc gatctgctcg cggagagagt
     7741 cgagctcgca accaaggcct cgcaggagct tttcgatcgc gttacgtcaa aaattctggc
     7801 caaaacggtc gcccaaagca gcgagatggc cgagaacacg ccgcccgctt tcagcgtagt
     7861 accgcctccc acagcgccgg ctgtccccca tgagcaaaaa ccccaaagta acggcagtaa
     7921 acgtctgtcc gaagcgaaag ccccaacgcg gagttcggcg ccagatctgt caaccgaact
     7981 cgaagtcaag aatccgcgcg gcaatcgcga gtacatcctg ctcgtagaag atgaaacgca
     8041 ggtggcggaa gtcgcggcag agatgctggc cgaagaaggc tacaaggtca tcgtcgcgca
     8101 cgacggcttt gaggccctga aaatttaccg caacgtcggc aaacagatcg gcctcgtcat
     8161 tctggattac ttcctgccag tgatggatgg ggatgcggtg ttcgaagagc tgcgcgcgct
     8221 gaattcgcac gtcaacgtcg tactcagcag cgggttcgcc gagcaacaga agatcagcgc
     8281 aatgctcgcg cagggactgc gcggattcat tccgaagcct tactcaagcg agaagctcct
     8341 cggccaggtg cgctcgacgc tagacgccgc gcgccaacgg ctggcctgac tgttccgcct
     8401 tacaagatcg acaagttcca cgagtcgttg aaccgaccga tatcggcgcc tgcgctggca
     8461 tcaatttagc ttctcgacag gaggtcgccc atgtctcgga ttgacgcttt cttcaaattg
     8521 atggccgagc aaaaagcctc ggacttgcat ctcgcgacaa acaacccgcc gatgctccgc
     8581 atcaacggcg acctggtgcg ggtcgatttt ccaccgctca aaaacgacga gctcaagacg
     8641 atggtttacg agatcgcgcc ggagggtaaa atcaaaatat tcgaggagac gtcggatgtt
     8701 gacttcggct acgaagtacc aggcgtcgcg cggtatcgcg ccaatttttt ccagcagaaa
     8761 cacggcatct ccgctgcctt ccgcttaatc ccctcaaacg tgctgacggt cgatgaactc
     8821 gggctaccgc cggtgctgaa aaaatttccg atgctgaaga aaggtctcgt gctcgtcacg
     8881 ggcccaaccg gttccggtaa gtcgacgacc ctcgcggcca tgatcgatta cgccaacctc
     8941 catcgtcagg accacatcat cacagtcgaa gacccgattg agttcgtgca tcgcagccag
     9001 aattgccttg tgcaacaccg cgaagtcggt gtgcatacgc ggtcgtttgc ggccgcgctg
     9061 cgcggtgcgt tgcgcgaaga ccccgacatc gttctggtcg gcgaaatgcg cgatctggaa
     9121 accatcgagc tggcgctcac cgcggccgcg accggtcacc tcgtgttcgg cacgttgcac
     9181 acctccaacg ccgccaaggc ggtcgatcgc gtgatcgatg ttttcccgac gcatcagcaa
     9241 aaccagatcc gcacaactct ggccgaatcg ctcaagggag tgatcgcgca gaatcttttc
     9301 aagcgcattg atcaccctgg ccgtgtcgcc gcgctcgagg ttctggttgt cgacatggca
     9361 attgcgaacc tgatccgcga gggcaaaacg caccaaatcc ccggcatgat tcaagtcggc
     9421 aagaagaaag gtaatcagcc tctcgacgac gcgatcatgg acctcttgcg caaggcgatc
     9481 atcagccccg aagaggccta cgacaaatgc atcgacaaga aaaaattccg tcaattcctg
     9541 aaagaaccgc ctgtcgacgg cagcgaagag taaaatgcga acaccggatc tggacaaaat
     9601 cttgtcggcg atgctgaaca cgtatgacgg catctccgat ctaaactttt ccgtcggtca
     9661 cccacttcag gtcgaagact tcggagagct gaaatcggta tttgtcgatc cgccaattga
     9721 agcgctcaca ccgtatcaaa ctgaacaggt cgcgctcacg ttaatgcagg gaaatcgccg
     9781 gctcatttac gattatctga ccggcggctc atgcgactgc agttacgcgc tccacgatca
     9841 ggcgcgcttc cgcgtcaaca tcttccggca acagggacat ttctccgtcg tcctgcgcaa
     9901 actgcaaggt gaggttccga aaatcgagtc gctccagttg ccgcccatct tccacgagat
     9961 cgcgaaggag aagactggac tggtgctcgt gaccggcgcc accggaagcg gcaagaccac
    10021 tacgttggcg gcgctgatca acgagatcaa tcacacccag ccggtgcaca tcgtcacgtt
    10081 ggaggacccg atcgagtacg tgcatccaaa ggcaaaagcc accatcaacc agcgcgagct
    10141 cggacacgac tttaacaatt acccgaacgg cttgcgcgct gcgttgcgtc aggcgccgaa
    10201 agtcattctg gtcggtgaaa tgcgcgaccg cgccaccgtc gagatcgcgc tcatggccgc
    10261 ggagacgggc cacctcgtct tgagtacgct gcatacggtc gacgccggcc agtcgatcaa
    10321 tcgtattctt ggtctgtttt ccatcggcga agagaagcaa ctgcgcattc gtctggccga
    10381 tgttttgcgt tacgtcgtca gccagcgcct cgtgccgaaa gtccgcggtg gtcgtcagtt
    10441 actgaccgaa gttctcgggc acaacctgcg cacgcgcgaa gccgttgccc ttggtgaagg
    10501 cgaacatcgc agcttctacg agatcattga agcgagctcg ccatttggct ggatgacctt
    10561 cgaccagtcg atcctcagcg cgtatgagaa cgacctcatc agcgaagaga cggcgaagct
    10621 tttcgcctcg aaaaaaggcc gcatatcgcg cggcatcgat ttaatccaga aggcacgcgg
    10681 ggtcgatacc gagctggatt ccggcctgcg cctcgacctg ccggcgaacg cctttcgcta
    10741 agatcgacat ctagtcatgg aaatcgaaac cgatcccacg tttctcgaca tcggcgacaa
    10801 caccgcgctc gtttgcgtcg atcaccagca ataccagaag attgtcgtgc cgcagctcat
    10861 tgatctcggt tacaaggtcc acctcgggtt gttcgaggaa gatgtcatcc tcaagctgca
    10921 gacctataat tacgatgtgg tcgtcgttta cgagaacttc aaggaatcga ctgcgcaaac
    10981 caatcccgtg ctgcgcgcgc tgagccggtg tcccgatgcg cgccgccgcg agcatttcgt
    11041 cgttttgctt agccatcggt ttccgaccaa cgacgcgatg agcgcgtttg tgcagagcgt
    11101 cgaccaaatc gtcaacatta gtgatctcgg caacttcaaa ccgatcgtgc gacgtggcgc
    11161 cgtgcagcat cgagagcttt atcactcatt caacgagatg gtgaagacgg tacagtcgat
    11221 ctgatctcgc tgtgcggcgg cacacgttgc gcgacggcac cgaccgcatg tgaataactg
    11281 cacgattatt cacattgaaa ttttcggcgt cgactccaat tgaattgcca gcgccagcga
    11341 ccgtaatatt ctttgcattc gtcgccaatg aagcactcga gctggatccg cttccaatgg
    11401 gatttgagcg cgttcccagc caacggttcg cccttgccac cacgctacca gatcgcaccg
    11461 gcgtccggcc acgatgtaga gcagctgcgc aaacttttct ccaccgcctt cacgctcgac
    11521 tcgacctgga acgcggccgt ggacgaagtg ctggcaaccg tgcaaatgtg gctcgagcgc
    11581 gctttcgcat cacgcacaag cacttgcctg gcgctacgtc actgcacacg gattattggc
    11641 gcgtctgttg tactcggcga accggaagcc gataatcacc tcacgccggg tccatgcatt
    11701 ttag
//
LOCUS       DASTDG010000174         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_759230, whole genome shotgun sequence.
ACCESSION   DASTDG010000174 DASTDG010000000
VERSION     DASTDG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759230"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFO30_05680"
     CDS             complement(<1..306)
                     /locus_tag="VFO30_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418812.1"
                     /translation="MNYKEALAWLYSLQRFGIRLGLENIRRLIAEFVASASCRWDTGW
                     KPVPRDTCVIHVAGTNGKGSVCAMIEAICRAAQYRTGLFTSPHLVTFRERIRVNGEMI
                     "
     gene            complement(303..1160)
                     /gene="accD"
                     /locus_tag="VFO30_05685"
     CDS             complement(303..1160)
                     /gene="accD"
                     /locus_tag="VFO30_05685"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET9418813.1"
                     /translation="MPDNMAIFKKPPPGAGGKKKRDIPQGLFQKCPGCGEVVHEIELA
                     QNQRVCPRCDYHFPQSARERLEHLMDAETFVEMDADLKSIDTLQFHGMVTYKDRLKKY
                     QQATGLSDAVISGYGLIDGYKVAIAVMDFSFLAATMGSVVGERITRTIEYGTRERSAV
                     IIVSASGGARMYEGMLSLMQMAKTSGALARHAEQRLPYISVLTNPTTAGVMASFASLG
                     DVIIAEPKSMIGFAGPRVIKETTHQDLPKAFQTAEFLVEHGLIDMIVHRRKMRDEISR
                     LLSYLSLAP"
     gene            complement(1153..1893)
                     /locus_tag="VFO30_05690"
     CDS             complement(1153..1893)
                     /locus_tag="VFO30_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET9418814.1"
                     /translation="MAEITRMRLKADFLGAIASLLYPPACAVCSENVDAGEYLCASCD
                     AKLTRIAPPFCAKCSEPFPAAISDTFTCANCTHRRLHFEAAVAAYRSRGIVRQVLLDF
                     KYGRQIHLRHLVARWLLAALGDERLRNATFDIIVPVPLHPARERERGFNQSALIAELL
                     SAHTALPLKPALERIRYTTTQTAFDRAERIQNLRGAFRLRKTADVRGLRVLLVDDVLT
                     TGSTLSECARVLQTLGARSIHAATAARA"
     gene            complement(1866..2762)
                     /locus_tag="VFO30_05695"
     CDS             complement(1866..2762)
                     /locus_tag="VFO30_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9418815.1"
                     /translation="MALRQNLTLAGLKDAARLFIYLGASILLGALLAPLLFWSAQACA
                     PQLFARVDFESFFHRALLIALAALLFPFTRWVRGRRWQDLQLEPNRQWSRDVAAGFLL
                     ALAPLLCCGALLVVAHLFSLRHVVNWSGLAKVIGAAVAVPLIEESFFRGLVLGILLRS
                     GHKYIAMVLSSALFSIVHFFKAPEQTSPIVTWSSGFDSIAHSFSKFANPTVVAAGFTT
                     LFLIGYIMADARVKTRSLWLPIGLHCGWIFGSGLFNRIAHREVIALPWVGKNLLVGIV
                     PLAVAALTWLLMRRWLRSRACD"
     gene            2756..3016
                     /locus_tag="VFO30_05700"
     CDS             2756..3016
                     /locus_tag="VFO30_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418816.1"
                     /translation="MPRQHIWTEKDAEGRKREIRATKFGRAWRFQSKDAHQSGWTYHD
                     PPLLDHLLRLKQIIARKYQRRRASADDVASVEKLLRDHEDNE"
     gene            complement(3028..3414)
                     /locus_tag="VFO30_05705"
     CDS             complement(3028..3414)
                     /locus_tag="VFO30_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9418817.1"
                     /translation="MTRAGHLRKSATDCERILWRRLRNRNFAGYKFRRQHPIDRYLLD
                     FYCPAVKLAIELDGSGHGYRLRESRDLAREQFLARQGIAVVRFWNHQIREELDSVLQA
                     IWLALEKRTPNNPSPQSSPFRKGRGG"
     gene            3544..4827
                     /gene="bioA"
                     /locus_tag="VFO30_05710"
     CDS             3544..4827
                     /gene="bioA"
                     /locus_tag="VFO30_05710"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET9418818.1"
                     /translation="MTEWNTEQLVAADKQLLWHPFTNVRDWCAPEHEPLVLVQGRGAV
                     VRDSRGREYIDGNSSIWTNIHGHNHPHINAAIREQLERVAHTSFLGFTNPAAIRLAEA
                     IVDLLPGDSLKRVFYSDDGSTAIEVALRIAAQYWRLRGSRRHQFIAFQHAYHGDTAGA
                     AALGAAEMFRTGLTHWNFPAVHVPDLKTLETISQTNTETIAAVVIEPLIQGAAGMRVW
                     PPGTLRGVREWCDRTGALLIVDEVMTGFGRTGKMFAIEHEAVVPDIIVLGKGLSGGYL
                     PLAMTVVSGEIFSRFDGSVAEGRALAYGHSYTGNSLGCAAAKASLEVFVTENVLERLQ
                     PKIEQMRFELGTIAGLASVREVRRCGFIAGIELRSPQDVELAAKVCLAARARGLLTRP
                     IRNVIVFMPPLCITAPQLSVATSAIYDSICAICDL"
     gene            4824..>5075
                     /locus_tag="VFO30_05715"
     CDS             4824..>5075
                     /locus_tag="VFO30_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HET9418819.1"
                     /translation="MSTSATKKMRLDNLLVARGFFDSRERAQRAIMAGDVRIAERSVS
                     KASELVAPNASISIAHRRQYASRGALKLAGVLDSFQIEVA"
BASE COUNT         1083 a         1442 c         1534 g         1016 t
ORIGIN      
        1 aatcatctcg ccgttgacgc gaattcgctc gcggaatgtc acgagatgag gcgaggtgaa
       61 aagaccggtg cgatactgcg cggcgcgaca gatcgcctcg atcatcgcgc agacggagcc
      121 tttgccattt gtgccggcca cgtgaataac gcacgtgtcg cgtggcacgg gcttccagcc
      181 cgtgtcccac cggcaagatg ccgatgccac gaactcggca atgagccggc gaatgttttc
      241 cagaccgagt ctgatgccga agcgctggag gctgtaaagc caggcgagcg cttctttgta
      301 gttcacggcg ccagcgacaa gtagctgagc aagcggctga tctcgtcgcg catttttctg
      361 cggtgcacga tcatgtcgat taagccgtgc tccacgagaa attccgccgt ttgaaatgct
      421 ttcggcaagt cctggtgggt cgtttccttg atcacgcgcg gcccggcaaa gccgatcatg
      481 cttttcggct cggcgatgat gacgtcgcct aacgaggcaa agctggccat gaccccggcg
      541 gtcgtcggat tggtgagcac cgaaatgtac ggcagccgct gctcggcgtg ccgcgcgagt
      601 gcgccgctgg ttttcgccat ttgcatcagg ctcaacattc cttcgtacat gcgcgcacca
      661 ccggaggcgg atacgatgat cacggcggat cgttcgcgcg tgccgtattc gatcgtgcgc
      721 gtgattcgct cgccgaccac cgagcccatc gtcgcggcga ggaaactgaa atccatgacc
      781 gcgattgcga ccttatagcc gtcgatcaat ccatagccac tgatgacggc gtcgctaaga
      841 ccagtcgctt gctgatattt tttcagtcgg tctttgtacg tcaccattcc gtgaaactgc
      901 agggtgtcga tcgatttcag gtcggcgtcc atctcgacga acgtctccgc gtccatgaga
      961 tgctcgagcc gttccctggc cgattgcgga aaatgatagt cgcagcgcgg gcaaacccgc
     1021 tggttctgcg ccagctcgat ctcgtgcacg acctcgccgc agccgggaca tttttggaac
     1081 agcccctgcg gaatgtcgcg cttctttttt cctcctgcgc cgggcggcgg ttttttgaag
     1141 atggccatgt tgtcaggcac gagctgccgt ggccgcgtgg atggaacgcg ctcccagcgt
     1201 ttgcagaacg cgcgcgcatt cgctcagcgt cgagccggtt gtcagcacat cgtcaacgag
     1261 gagcacgcgc aagcctcgca cgtcggccgt ttttcgtaaa cgaaatgcac cgcgcaaatt
     1321 ttggatccgc tccgcgcggt cgaaggccgt ttgcgtggtg gtgtagcgaa tgcgctcgag
     1381 cgctggcttc agcggtaatg ccgtgtgcgc gctcaacagt tcggcgatca gcgcgctttg
     1441 gttgaaaccg cgctcgcgtt cacgcgccgg atgcagcgga acgggaacta tgatgtcgaa
     1501 cgttgcgttg cggagtcgtt cgtcgccgag ggcggcgagg agccaacgcg cgaccagatg
     1561 gcgaagatga atttgccggc cgtacttaaa atcgagcaga acctgccgaa cgattccgcg
     1621 gctgcgataa gccgcgactg cggcttcgaa atgcaaccta cgatgagtgc aattcgcgca
     1681 ggtgaaggtg tcgctgatcg cggccggaaa gggctcggag cactttgcgc aaaatggcgg
     1741 cgcaatccgc gtaagcttcg catcacagct ggcgcaaaga tattcgcccg cgtcgacatt
     1801 ctcagagcaa accgcgcacg ccggcgggta aagcaaagac gcaatcgcac cgagaaaatc
     1861 agcctttagt cgcatgcgcg tgatctcagc cacctgcgca tcagtaacca agtcagcgcg
     1921 gcgacggcga gcggtacgat gccgactagc aagtttttgc cgacccaagg cagagcgatg
     1981 acttcacgat gagcgatccg attaaacagg ccgctgccga agatccatcc acaatgtagg
     2041 ccgatgggta gccagagtga tcgggttttc acccgcgcat cagccatgat gtatccgatc
     2101 aggaacaatg tcgtgaaccc cgcggcaaca actgttggat tggcgaactt ggaaaaggaa
     2161 tgtgcaatgg agtcaaaacc ggagctccat gtcacgatcg gtgatgtttg ttccggcgct
     2221 ttgaaaaaat gaacgatgga aaacaatgct gagctcagca ccatcgcgat gtacttatga
     2281 ccgctcctta gcaggatgcc gagcacgagc ccgcggaaga aactttcctc aataagcggg
     2341 accgcaacgg cggcaccgat cacttttgcc aggcccgacc aattgacgac gtgacgcaat
     2401 gaaaacagat gcgctacgac caggagtgcg ccgcagcaga gcaggggagc cagggcaagt
     2461 aaaaagccgg ccgcaacatc gcgcgaccat tggcggtttg gctcgagttg aagatcctgc
     2521 catcgccgcc cgcggaccca ccgtgtgaat ggaaatagta acgcggcgag cgcgatcagc
     2581 agcgctcgat gaaagaaact ttcgaagtcg acgcgggcga agagctgcgg agcgcacgcc
     2641 tgcgccgacc agaacagcag cggggcaagc agcgcgccga gtaggatgct agccccaagg
     2701 tagatgaaca atcgcgctgc gtccttcaag cctgctaatg taagattttg ccgcaatgcc
     2761 acgtcaacac atatggacag agaaagacgc ggaggggcgc aagcgcgaga tccgcgcgac
     2821 gaagttcggc cgcgcgtggc gttttcaatc gaaagacgcc caccaatccg gctggaccta
     2881 tcacgatcct ccgttgctcg atcatctgct gaggttgaag caaatcattg cgcgaaaata
     2941 tcagcgccgc cgcgcttcag ccgacgatgt tgcgtcggtg gaaaaactac ttcgcgacca
     3001 cgaggacaat gaataagcac gacttcatta accgcctctc ccttttcgaa agggagagga
     3061 ttgaggtgag ggattgttcg gcgtgcgttt ttcgagcgcg agccagatcg cctgcaggac
     3121 gctatcgagt tcttcccgaa tctggtggtt ccaaaagcga actacagcaa ttccctgtct
     3181 ggccagaaac tgttctcgtg ctaaatctcg actctcgcgc agtcggtagc catgccctga
     3241 gccatccagt tcgatggcaa gcttcacggc cggacaatag aaatccagca agtaacggtc
     3301 gatcgggtgc tgtcgccgga acttatagcc tgcgaagttt cggttgcgaa ggcgacgcca
     3361 cagaatgcgt tcgcaatccg tcgcactttt gcgcagatga ccggcgcgag tcattgggcg
     3421 attctttggc gattgcgatt tcttacgcga atcaaaatcg gccacccctc acctcaatcc
     3481 tctccccgca gccggaggag aggcggatta gggcgtgcat ttggtcggag taggtaatga
     3541 tcaatgactg agtggaacac tgagcaactc gtcgcggccg ataagcagtt actctggcat
     3601 ccgttcacga acgtgcgcga ttggtgcgcg ccggaacacg agccgctggt gcttgtccaa
     3661 ggacgcggcg ctgttgtgcg tgacagccgc gggcgtgagt atatcgacgg taattcgtcg
     3721 atttggacca acatccacgg gcacaatcat ccgcacatca acgcagcgat ccgcgaacag
     3781 ctcgagcgcg tggcacatac ctcgtttctt gggtttacaa acccggcggc gattcggctc
     3841 gccgaagcga ttgtcgatct tttgccgggc gactcgctaa agcgcgtgtt ttattctgac
     3901 gacggatcga cggcaatcga ggtcgcgctg cgcattgccg cgcaatactg gcgattacgc
     3961 ggctcgcggc ggcatcagtt catcgcgttt cagcacgctt accacggcga cacggcgggg
     4021 gcggcggcac tcggcgccgc ggagatgttt cgcactggtc tgacccactg gaatttcccg
     4081 gcggtgcatg tgccggattt gaaaacgctc gaaaccattt cgcaaacaaa cacagagaca
     4141 attgccgcag ttgtgatcga accgctcatc caaggcgcag ccggaatgcg cgtgtggccg
     4201 ccgggcactt tgcgcggagt tcgcgagtgg tgcgatcgca ccggcgcact gttgattgtg
     4261 gatgaagtga tgaccggctt tgggcgcacg ggaaaaatgt ttgcgattga acatgaagct
     4321 gtcgtgccgg acatcattgt ccttggaaag ggactcagcg ggggttatct gccgctggct
     4381 atgacggtgg tgagcgggga aattttttcg cgattcgacg gctcggtggc cgagggcaga
     4441 gcgctcgcct acgggcacag ctacaccggt aattcactcg gctgcgccgc ggccaaggcc
     4501 agcctcgaag tgttcgtaac cgaaaacgtg ctcgaaagat tgcagccgaa aatcgaacag
     4561 atgcgatttg agctgggaac aatcgcaggt ctcgccagtg tgcgcgaagt gcgacgatgc
     4621 ggttttattg ccggaattga gttgcgctca ccgcaagatg tcgaacttgc ggctaaggtt
     4681 tgtctcgccg cgcgcgcgcg cggtttactg acgcgtccga tccgcaacgt catcgttttc
     4741 atgcccccgc tttgcattac tgcgccgcaa ctgtcggtcg cgaccagtgc gatttacgac
     4801 tccatctgcg cgatctgcga tttatgagca caagcgcaac aaagaaaatg cggctcgaca
     4861 atttgttggt cgcgcgcggc tttttcgaca gccgagagcg cgcgcagcgg gccattatgg
     4921 ccggtgatgt gcgaatagcg gagcgcagcg tttccaaggc ttcggagtta gtcgcgccga
     4981 acgcgagcat ttccattgcg caccggcggc agtacgcgag tcgcggcgca ctcaagctcg
     5041 ccggggtcct cgattctttt cagatcgaag tcgct
//
LOCUS       DASTDG010000175         6601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_437999, whole genome shotgun sequence.
ACCESSION   DASTDG010000175 DASTDG010000000
VERSION     DASTDG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437999"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..875)
                     /gene="dapB"
                     /locus_tag="VFO30_05720"
     CDS             complement(195..875)
                     /gene="dapB"
                     /locus_tag="VFO30_05720"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9418820.1"
                     /translation="MKRVRVSLVGAKGRMGRTIVDLAKAEPNIEIVAQCDFGDAIAPA
                     MENVDVTIDFSQPGAIEEICRATVAHGKPLVIGTTGHSPTQRELIEQTARLVPIVFAS
                     NFSVGVNALFALTRKAGEILRDFNVEIVETHHKMKKDTPSGTAKTLAELLKEARKIDD
                     EVAIQSIREGEVVGEHTVIFTGPGERLEVTHRAASREIFARGALRAAEWIVGKPPGLY
                     SMADVLGL"
     gene            complement(875..1771)
                     /gene="dapA"
                     /locus_tag="VFO30_05725"
     CDS             complement(875..1771)
                     /gene="dapA"
                     /locus_tag="VFO30_05725"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9418821.1"
                     /translation="MFRGTFTALVTSFRNGDVDPAAFEKLIEAQIAAGITGIVAVGTT
                     GESPTLTHDEKEELIARAGKVANGRCKLLAGTGSNSTRDTIAATKFAEKAGVDGALLV
                     APYYNKPSQEGLFRHFKAIADATSLPIVLYNIPGRCSVDILPETVVRLAESCRNIVSI
                     KEASGSVERVGELRRRLPDQFTILSGDDSLTLPFMSVGAAGVVSVASNLFPAEVCMLV
                     RACESGDFQSAKRLHRELLPLFKDLFVEPNPVPVKTALGWRGVMSGEVRLPLCEMSEA
                     NQARLRKTLEEFEREMKSNTLA"
     gene            complement(1874..2680)
                     /gene="dapF"
                     /locus_tag="VFO30_05730"
     CDS             complement(1874..2680)
                     /gene="dapF"
                     /locus_tag="VFO30_05730"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9418822.1"
                     /translation="MQLRFTKMNGAGNDFVMIDNRAGDLQLQPEQIVRFCDRHRGVGA
                     DGILLLEPAQNGADFRMRYYNRDGAEAEMCGNGARCFARFAKKVASAHDNVSFETPAG
                     IIGARVHDELVTLNMSEPTDLRLNVRLEIDGEETYVHYINSGVPHVVVPVARVDVVHV
                     TTQGAAIRRHKVFAPKGANVNFVEKRGPKRIAIRTFERGVEDETLACGTGVVASALLF
                     SMLENVSGPIDVLVRGGNELRVGFDRAGDRFRDVTLTGPAEFVFEGTIEL"
     gene            2859..3605
                     /locus_tag="VFO30_05735"
     CDS             2859..3605
                     /locus_tag="VFO30_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418823.1"
                     /translation="MRKVVAQMCACLAAQLARAENFNALILEQVSKMPAGGKYSVSHF
                     ATIKLQSAAHFESGKFFVIPTAPYPSFCSGATYLVFIKTLEALRDRGQLQLDFATLNQ
                     LIIRDQQDGEGIWGRWNANGPGTARLFHELGLGRNFTDFGQAQPGDFMKIFWNKNVGR
                     RESGHSVIFLGLTNRPDGQYVRFWSSNIPRGYGEKEVPRNKIAFAIFSRLETPANLTR
                     IHNAPETDTYLASLLRKNSNFAEALRMCGI"
     gene            3693..4352
                     /locus_tag="VFO30_05740"
     CDS             3693..4352
                     /locus_tag="VFO30_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9418824.1"
                     /translation="MSDRQKTLTINEIYESIQGESTWAGERCVFVRLTFCNLRCNYCD
                     TEYAFYEGKKQTLNEIVDLVAAFDCPLVEVTGGEPLLQKNVLPLMSILCDRGHTVLLE
                     TSGAHDISPVDPRVHRIMDLKTPGSGEVDKNLWSNIDHLTSLDEVKFVIGSREDYDWS
                     CDKIQRFDLTKRCHAVLFSPIFGRIDPRQIVGWMLADKLDVRFQLQMHKFIWSPTQRG
                     V"
     gene            4705..5718
                     /gene="gmd"
                     /locus_tag="VFO30_05745"
     CDS             4705..5718
                     /gene="gmd"
                     /locus_tag="VFO30_05745"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9418825.1"
                     /translation="MKKALITGITGQDGSYLADLLLEKGYEVHGIIRRASTFNTSRID
                     HLYADPHINGVRLFLHYGDLGDSVNLVKLLYELKPDEIYHLGAQSHVRVSFDIPEYTS
                     DITAVGTIRILEAIREVGIRSRFYEASSSEMFGKAQEVPQSEKTPFWPRSPYGVAKVF
                     SYWATINYRESYGLHASNGILFNHESPRRGETFVTRKVSRAVAAIKYGLQHELFLGNL
                     DAKRDWGYAPEYVDGMWRILQHDSPQDFVLATGETHSVREFVEAAFAHVDLDWKEYVK
                     HDPRYERPAEVDLLVGDASKAKKVLGWEPKVRFHDLVRIMIDADLQLLSRETPRKHLG
                     KLP"
     gene            complement(5802..6479)
                     /locus_tag="VFO30_05750"
     CDS             complement(5802..6479)
                     /locus_tag="VFO30_05750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9418826.1"
                     /translation="MKIDIQCGMRFQRMSHRQDADATGKIDIRALFALVIPLLVSSCA
                     APDRQHQIVISTADQKLALLDGGKITAVYPVSTSKFGLGDWPGSYCTPVGELEIAQKI
                     GQGAPTGMVFKDRRDTGEIVWADAPGRDPIVTRILWLRGREAQNANAFRRNIYIHGTP
                     EERDIGLPVSYGCIRMRSSDVIHLFSTVGIGTRVTIVDLPMSSMIPDFSSAAQLAKSG
                     TSSAALR"
BASE COUNT         1341 a         1994 c         1845 g         1421 t
ORIGIN      
        1 accggttccg tctcataaag cggtgattgc agaacgggcg gcgaaacgtc ggaaagatcg
       61 acaattgcct ttcctgccgc acgcaaattc gccagccgat cgcccagatt cgatcccagc
      121 gcgaccgcag ctctcatcgt agcgcgagca tcccgcttgc gatctcagaa tccgcaacct
      181 ggagggttgc gctactatag tccgagcaca tccgccatcg aatagagtcc cggcggtttt
      241 cccacgatcc attccgccgc ccgtaatgcg ccacgcgcaa agatctcgcg gcttgccgcg
      301 cgatgcgtca cttccaatcg ctcgcccggt ccggtgaaga tcaccgtgtg ttcgcccacg
      361 acttcgcctt cgcggatcga ctgaatcgca acttcatcgt cgatcttgcg tgcctctttc
      421 aatagctcgg ccagcgtttt cgccgtgccg ctcggtgtat cttttttcat cttgtgatgt
      481 gtttcgacga tttcgacatt gaaatcgcgc aggatctcgc cggctttgcg cgtcagagcg
      541 aacaacgcgt tcactccgac gctgaaattc gacgcgaaga caatcggcac caagcgcgct
      601 gtctgttcga tcagctctcg ttgcgtcggt gaatggccgg tcgtaccaat gacgagcggt
      661 ttgccatgtg cgaccgtcgc gcgacaaatc tcttcaatcg cgccgggctg gctgaaatcg
      721 atcgtcacat cgacgttctc catcgccggt gcaatcgcgt caccaaaatc gcattgcgcc
      781 acgatctcga tattcggttc ggcctttgca agatcgacaa tcgttcggcc catccgcccc
      841 ttcgcgccga cgagcgagac acgcacgcgt ttcatcacgc gagcgtgttg gatttcattt
      901 cgcgctcaaa ctcttcgagc gttttgcgca gccgcgcctg attcgcttcg ctcatttcgc
      961 agagcggcag acgcacttcc cccgacatca cgccgcgcca gccgagcgcc gttttcaccg
     1021 gcaccgggtt tggttcgacg aacaaatctt tgaacagcgg caacaattcc cggtgcaatc
     1081 ttttggctga ttggaaatcg ccggattcac atgctcgcac gagcatgcaa acctcagccg
     1141 gaaacaaatt ggaagcgacg ctcaccacgc ccgccgcgcc gaccgacatg aatggaagag
     1201 tgagtgaatc gtcgccgctg aggattgtga attggtcggg caaacggcgg cgcaattctc
     1261 ccacgcgctc gacactgccg ctcgcttcct tgatcgagac gatgttgcgg cacgattcgg
     1321 ccagcctaac gacggtttct ggaagaatat caacagagca gcggccggga atgttgtaga
     1381 gcacgatcgg caacgaagtg gcgtcggcga tggctttgaa atgccgaaat aagccttcct
     1441 gactcggctt gttgtaatac ggcgcgacga gcaacgcgcc gtctacgccg gctttctcag
     1501 cgaattttgt ggctgcaatg gtgtcacgcg tggagttcga tcctgttccg gccaggagct
     1561 tgcagcggcc attcgcaacc tttccggcgc gcgcgatgag ttcctctttc tcgtcgtgcg
     1621 tcaacgtcgg cgattcaccc gttgtcccga ccgcaacgat cccggtgatt ccggcggcga
     1681 tttgcgcctc gatcagcttc tcaaacgccg caggatcgac atctccattg cgaaaggatg
     1741 tgaccagcgc cgtaaacgtg ccgcgaaaca ttgccgcaat tgtaggggcg gctgtcctca
     1801 gccgcaatca tctgtagtta cgggcctgtg gcccgtctga tggttgcaga ccgacggcgc
     1861 atagcgccgg tggctacaac tcgatcgtgc cttcgaacac gaactcggct gggccggtca
     1921 gtgtcacgtc gcggaaccgg tcccctgctc tgtcgaagcc gacgcgtagc tcgttcccgc
     1981 cgcggacaag cacgtcgatc ggtccgctca cgttttcgag catggagaag agcaaagcgc
     2041 tggcgacgac tccggtgccg caggcgagcg tctcatcttc gaccccgcgc tcgaacgtgc
     2101 ggatcgcgat tcgcttcggc ccacgtttct cgacgaagtt cacgtttgcg cctttgggag
     2161 caaacacttt gtgccggcga atggccgctc cttgcgttgt cacatgcacc acgtcaaccc
     2221 gtgcaacggg cacaacgacg tgcggcacgc cgctgttaat gtagtgcacg tatgtttcct
     2281 cgccgtcgat ctccagccgc acgttcaggc gcagatccgt cggctcgctc atgttgaggg
     2341 tgaccagttc atcgtgcacc cgcgcgccga tgatgccggc tggcgtttcg aatgaaacgt
     2401 tgtcgtgcgc actcgcgact ttcttggcaa aacgagcgaa acagcgcgcg ccattgccgc
     2461 acatttctgc ttcagcgccg tcgcggttgt agtaacgcat ccggaaatcg gcaccgttct
     2521 gcgccggctc gagcagaagg attccgtctg cgccaacacc gcgatgccga tcacagaacc
     2581 gcacgatctg ctccggctgc agctgcaggt cgccggcgcg attgtcgatc atgacaaaat
     2641 cgttgcccgc gccgttcatt ttcgtgaagc gcagctgcat atgtcgatct tacaccttga
     2701 ctgtcgtctg tcatgtccag cgaagtcgct tgccgcgccg tagctttagc cgtaggcggg
     2761 agacatctct tgctgtttaa caacgagaga ttcctcgact ccgctcggaa tgacaaagat
     2821 gcgttgcctg taagtcattc atcattttag ttgcgaaggt gcgcaaggtt gttgcacaaa
     2881 tgtgcgcgtg tttggcggcg cagctcgcgc gcgcggaaaa tttcaatgcg ctcattctcg
     2941 agcaggtgag caaaatgccg gccggcggca aatattccgt ctcgcatttt gccacaatca
     3001 aattgcaatc cgccgcacat tttgagtccg gaaaattttt cgtcatcccg actgcgccct
     3061 atccgagctt ttgctcgggc gcgacgtatc tggtgttcat caaaacactc gaggcgctgc
     3121 gcgaccgcgg ccagttgcaa ctcgattttg ccacgctcaa tcagctgatc attcgcgatc
     3181 agcaggacgg cgaaggcatt tggggccgct ggaacgcaaa tggccctgga accgcccgcc
     3241 ttttccacga gctcggcctc ggccgcaact tcaccgattt cggccaggcg cagcccggcg
     3301 atttcatgaa aatcttctgg aacaaaaacg tcggccggcg cgagagcggc cactcggtca
     3361 tttttcttgg cctaacgaat cgtccggacg ggcagtatgt tcgcttttgg tcgagcaaca
     3421 tcccgcgcgg ctacggggag aaagaagtgc cacggaacaa aatcgcgttt gcgattttct
     3481 cgcggttgga aacaccggcg aatctcacgc gcatccacaa cgcgcctgag acggacacat
     3541 accttgcttc actgctgcgg aagaactcga atttcgccga agcgctgcgg atgtgtggaa
     3601 tttgagcggt cgttgaaccg ttgaatagtt gaagcgttaa agaagtagag acgaagccgc
     3661 ttcaacggtg taacacttca acccttcaac ggatgtcaga tcggcaaaag acgctgacga
     3721 tcaatgaaat ctacgagtcg atccagggcg agagcacctg ggccggcgag cgatgcgtgt
     3781 ttgtgcgcct gaccttctgc aatctgcgct gcaattattg cgacaccgaa tacgcgtttt
     3841 acgagggcaa aaagcagacg ctgaacgaaa ttgtcgatct tgtcgccgcg ttcgattgcc
     3901 cgctggtcga ggtcaccggg ggcgaaccgt tgctgcaaaa gaacgtgctg ccattgatgt
     3961 cgatcttgtg cgaccgcggc cacaccgtct tgctcgagac gagcggcgcg cacgatatct
     4021 cgccggtcga tccgcgcgtc caccggatca tggacctgaa aacgccaggc agcggcgaag
     4081 tggacaagaa cctctggtcg aacatcgatc acctcacgtc cctcgacgaa gtgaagttcg
     4141 tcatcggctc gcgtgaagat tatgactggt cgtgcgacaa aatccagcgc ttcgacctaa
     4201 caaaacgttg tcacgccgtc ctcttctcgc ccatttttgg ccggattgat ccgcggcaga
     4261 tcgtcggatg gatgctggcc gacaaactcg atgtccggtt ccagctccag atgcacaagt
     4321 tcatctggtc gcccactcag cgcggcgttt gacgccccct cactcggcta cttccgccgt
     4381 tctgctcgcc ttgaagtttg aggtgaggcc acggtatctg ccagtggccg aagcgcatcc
     4441 tgctttaact cgctctttgt tcggcgtgag ttggcgagcg cttcttcttt caacgcgcgt
     4501 tcgagcccgt cgaggaggac tgtagggtgg ttcgcagccg caaaaacctg gacaaccgac
     4561 acagactacc aagactacgc ctggtatgat gcggtccatt tgcggcgtcc gtcgcctcag
     4621 agcgcttgac aggccagcga gatacatctg cttcgtgaag ccgttgctcg cgcacaccct
     4681 ttgcttcaaa gcgagacgcc gctcatgaaa aaggcgctca ttactggcat caccgggcag
     4741 gatggaagct atctcgccga tctgttgctc gagaagggct atgaggtgca tggcatcatc
     4801 cgacgcgcca gcacgttcaa cacttcgcgg atcgatcatc tttatgccga cccgcatatc
     4861 aacggcgtcc gcctcttcct ccactatggc gacctcggtg attcggttaa tctggttaag
     4921 ctgctctacg aattgaagcc ggacgagatc taccatctcg gcgcacaaag ccatgtccgc
     4981 gtcagcttcg atattcccga atacacctcc gacatcactg ccgtcggcac cattcgcatt
     5041 ctcgaggcga tccgagaagt aggaatccgc tcgcgttttt acgaggcgtc gagcagcgag
     5101 atgtttggta aggcgcagga ggtgccgcag tcggaaaaga cgcccttctg gccgcgcagt
     5161 ccttatgggg tggcaaaagt gttttcatac tgggccacca ttaattaccg cgagagctac
     5221 ggcctgcatg cgagtaacgg gattttgttt aatcacgaaa gcccgcgacg cggtgagacc
     5281 ttcgtcacgc gcaaggtctc gcgcgcagtg gccgcgatta agtatggact gcagcacgaa
     5341 ctctttcttg gcaacctcga cgccaaacgc gactggggtt acgcaccgga gtatgtcgac
     5401 ggcatgtggc gcattctcca gcacgattca ccgcaagact ttgttttggc cacgggcgag
     5461 acccacagtg tgcgcgagtt tgtcgaggcc gctttcgcac atgtcgatct tgattggaaa
     5521 gagtatgtga aacacgaccc acgctacgaa cggcccgctg aggtcgatct gctcgtcggc
     5581 gatgcttcca aagcgaagaa agtgctcggc tgggaaccca aggtccgctt ccatgatctg
     5641 gtgcgcatca tgattgatgc ggatctgcaa ttgctttcgc gggaaacgcc gcgcaaacat
     5701 ctcggaaagc tgccgtgatg ctgtagaagt cgccgtcctc ggcggcaagt atattcactc
     5761 tccgccgcag cggacggcgg ccgttacagg gcggctacaa attaccgcaa cgccgccgat
     5821 gaggtgccgg attttgccaa ttgcgccgcc gagctaaagt ccggaatcat cgagctcatc
     5881 ggaagatcga caatcgtcac gcgcgtgccg attccaacgg tgctgaaaag atggatgacg
     5941 tcgctggaac gcatgcggat gcaaccgtag ctcaccggca ggccgatatc gcgctcttcc
     6001 ggtgtcccgt ggatgtaaat attacggcga aatgcgttcg cattctgcgc ttcgcgtccg
     6061 cgcagccaaa ggatccgtgt cacgatcgga tcgcgccccg gcgcgtcagc ccacacgatt
     6121 tcgccggtgt cacgtcgatc tttgaatacc atccctgtcg gcgcgccttg gccgatcttc
     6181 tgcgcgatct caagttcgcc gaccggtgtg cagtaacttc ccggccaatc gcccaaaccg
     6241 aatttggacg tcgacacggg atagacagcc gtaattttgc cgccgtccag tagcgcgagc
     6301 ttctggtccg cggtgctgat aacgatctga tgctgccgat ccggcgctgc acaagaacta
     6361 accagcagcg ggatgaccag cgcaaacagt gcgcggatgt cgatcttacc agtggcatcg
     6421 gcatcctgcc gatgagacat gcgctggaag cgcatgccac attgaatgtc gatcttcatg
     6481 cgcgattgaa acggaagaat ttttctgccg tctcggtcgt ggcctcggcg acttcttgca
     6541 aggaaacgcc gcgcgccgca gcaatcgctt ccgcgaccag ccgcgtgtgc gccggttcgc
     6601 a
//
LOCUS       DASTDG010000176         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_708638, whole genome shotgun sequence.
ACCESSION   DASTDG010000176 DASTDG010000000
VERSION     DASTDG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708638"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /gene="treZ"
                     /locus_tag="VFO30_05755"
     CDS             complement(<1..1503)
                     /gene="treZ"
                     /locus_tag="VFO30_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET9418827.1"
                     /translation="MRTPNVLAQNQRPTSGRKSDVRPQRRYPIGAEIISASGTHFRVW
                     APKVRQVEIVLESADKNGRHTHPLTREEGGYFCGVIEARAQQRYRFRLDKGEQLYPDP
                     ASRFQPEGPHGASVIVDPTQFEWSDENWRGVHLAGQILYEFHVGTFTPEGTWRAAADK
                     LPLLKEDGITLLEMMPIADFPGRFGWGYDGVNLFAPCHLYGTPDDLRLFVDRAHSLGL
                     GVILDVVYNHFGPDGNYLGAFSDYYISKRPANEWGDCINFDGEHSGPVREYFITNGRY
                     WIEEFHFDGFRFDATQSIHDKSPEYIIGAVGRAARQAAGDRSILLIAENERQETKLIK
                     AQSEGGDDLDALWNDDWHHTAMVALTGNREAYYTDYLGTPQEFISAAKYGYLFQGQPY
                     VWQGAPRGYPTEGRRPEQFVSFIENHDQVANTPFGERARFLTSPGRYRAMTALLLLGP
                     WTPLLFQGQEFGASAVFRYFSDVGDDELKEAVRKGRFEFLAQFPSVAAPEV"
     gene            complement(1683..>2636)
                     /locus_tag="VFO30_05760"
     CDS             complement(1683..>2636)
                     /locus_tag="VFO30_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9418828.1"
                     /translation="PIGEFELPEYELQKLSFPPILQMLDYIQRERFTEIIISTPGPVG
                     LTGLLAGKMLNLQTSGIYHTDFPQYVRILTEDSFLESVTWRYMHWFYGQLDAVFVNSE
                     EYRQSWIKRGFDPAKLKIFPRGLDTQLFHPKRRDPRFFEKFATSNGAVRLLYVGRVSR
                     EKDLDLLADAYRRLRDEGLLVQLFVVGEGPYSQAFAESLPEAVFTGYLRGEQLARAYA
                     SADVFVFPSTTDTFGNVIIEAQASGVPVVVSDSGGPKELVQNNENGLITRSHDVQDFT
                     RAVRNLVVDPALRERMGRRGRESVVDRTWPAAFRNFWAATE"
BASE COUNT          543 a          772 c          767 g          554 t
ORIGIN      
        1 cacctcgggc gctgccactg atggaaattg ggccagaaac tcgaagcggc ccttgcggac
       61 tgcttctttc aattcgtcat cgcccacatc gctgaaatat cgaaacactg ccgaagcgcc
      121 aaattcctgg ccttgaaaca aaagcggcgt ccatgggccg agcaagagta gtgccgtcat
      181 cgcgcgatag cgtccgggcg acgtgagaaa tcgcgcccgc tcgccaaatg gcgtattcgc
      241 gacctgatcg tgattttcga tgaacgagac gaattgctcg ggtcgccttc cttcggtggg
      301 ataaccccgc ggcgcgcctt gccagacgta tggttggccc tgaaaaaggt aaccgtattt
      361 cgccgcggaa atgaactctt gcggcgtccc gagatagtcc gtgtaataag cctcgcggtt
      421 gccggtgagc gcgaccatgg cggtatgatg ccagtcgtcg ttccaaagcg cgtcgagatc
      481 gtcgccgcct tcgctttgcg ctttgatcaa cttcgtttcc tgccgctcgt tctcagcgat
      541 caacaaaatt gagcgatcgc ccgccgcttg gcgcgccgcg cgtccgaccg cgccaatgat
      601 gtattcgggg gatttatcat ggatgctctg cgtggcgtcg aagcggaaac catcgaagtg
      661 aaactcctcg atccaataac gcccattggt gatgaagtac tcgcgcaccg gtccggagtg
      721 ttcgccgtcg aagttgatgc aatcgcccca ttcgttcgcc ggccgcttcg agatgtagta
      781 atcggaaaac gcgccgagat aattcccgtc cggcccgaag tgattgtaga caacatcgag
      841 aatgacgccc aggcctaacg agtgcgcgcg gtcgacaaac aagcgcaggt cgtcgggcgt
      901 gccgtagaga tgacacggag caaacaaatt gacgccgtca tatccccagc caaatcgccc
      961 cggaaaatcg gcgatcggca tcatctccaa caaggtgatc ccgtcttcct tcaacagcgg
     1021 cagtttgtcc gccgccgcgc gccaggtgcc ttccggcgtg aacgtgccga cgtgaaattc
     1081 gtacaggatt tggccggcaa ggtgcacgcc gcgccagttt tcgtcgctcc actcgaactg
     1141 tgttggatcg acaatcaccg atgcaccatg cggaccttcg ggttgaaacc gtgatgctgg
     1201 atcggggtag agctgctcgc ctttgtcgag gcgaaaccga taccgctgtt gcgcgcgcgc
     1261 ctcgataaca ccgcaaaagt aaccgccttc ttcgcgtgta agcggatgag tatggcggcc
     1321 gtttttgtct gcgctctcca gcacaatctc gacctgtcgc acttttggtg cccagacgcg
     1381 gaaatgcgtt ccgctcgcgc tgattatttc ggccccaatt ggataacggc gttggggacg
     1441 cacatcactc tttcgccccg aagtggggcg ctggttctgc gcgagaacat ttggcgttcg
     1501 catcggttga gaagatgcgg acgctcgcgc aattcacaac cgccggttta acaaaccggt
     1561 tttggcctag cgccagtggt ggcgaactta atttgatctg tgccgcaaga ttgcgcggtg
     1621 tgcagacaac ggcgccacga tgatgatcgt gcagtgcggc gcgacccgca cttggggctt
     1681 catcattctg ttgcggccca gaagttgcga aacgccgccg gccaggtgcg gtcgactacg
     1741 ctttcacgtc cgcgccggcc catgcgctcg cgcagcgcag gatcgaccac caggttgcga
     1801 acggcgcgcg tgaagtcttg cacgtcgtgc gatcgtgtga tcaatccgtt ctcgttgttt
     1861 tgcacaagct ccttcggtcc gccggaatct gaaaccacaa ccgggacacc ggaagcctgt
     1921 gcttcaatga tgacgttgcc gaaggtgtcg gtcgtgctgg ggaacacgaa aacgtcggcg
     1981 gaagcgtagg cgcgtgccag ttgttcgccg cggagataac cggtgaagac tgcttcgggc
     2041 agcgattccg cgaacgcctg cgaatatggg ccctcgccga cgacgaaaag ttgcaccaac
     2101 aatccctcgt cgcggagccg ccgatatgcg tcggccaaaa gatcgagatc cttctcgcgc
     2161 gaaacgcggc cgacatagag caggcgcacc gcgccgttgc tggtagcgaa tttttcgaag
     2221 aatcgcgggt cgcgccgctt cggatgaaac agctgcgtgt ccagcccacg cgggaagatt
     2281 ttcagcttcg ccggatcgaa tccgcgtttg atccagctct ggcggtactc ttcggaattc
     2341 acgaacaccg cgtcgagttg gccatagaac caatgcatgt aacgccacgt cacgctctcc
     2401 agaaaactgt cctctgtgag aatgcgcaca tattgcggaa agtcggtgtg ataaatgccg
     2461 ctggtttgca ggttcaacat cttccccgca agtaatccag tcagaccgac cgggcccggt
     2521 gtgctgatga tgatttcagt aaatctttcc cgctggatgt agtcgagcat ctgcaggatc
     2581 ggcgggaaac tgagtttttg cagctcgtat tccggcagtt cgaactcgcc gatcgg
//
LOCUS       DASTDG010000177         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_723671, whole genome shotgun sequence.
ACCESSION   DASTDG010000177 DASTDG010000000
VERSION     DASTDG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723671"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFO30_05765"
     CDS             <1..302
                     /locus_tag="VFO30_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418829.1"
                     /translation="AAFQFADANGRKIDIELRGNDGPPIGKIVINSTEAEFSVAPAQC
                     GDLLEISRGRPNEQRAGQLMPAQNYDLADLMTQELLRAGPHHIYLRAINCVRDLL"
     gene            complement(342..1529)
                     /locus_tag="VFO30_05770"
     CDS             complement(342..1529)
                     /locus_tag="VFO30_05770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9418830.1"
                     /translation="MRLAYLFERFPAFSQTFCYREVAELIRQGAKIDIYSLRRPTEEP
                     AQDWDEQIVERVHYLPEEKPLLEEVARMLRKRKLPPVRETIDHWGRKTDFLRLQQAVY
                     VGLPLQDAGIRHVHAHFAGMAARTAYWIENFFGIGFSFTAHANDIFAPRAFEISLEKL
                     VDRARFVVTETDYAANFLRERFAERGERIHRVYNGLVLVEFRRADFSAELPTIVSVGR
                     LIEKKGFPYLLESCRLLKERGHVFRCQIIGEGPLESDLRGRIAEFQLQNHVELLGPKP
                     QRDIVGYLAAAAVFALPSVIDAQGGMDNLPSVIMEAMAAGLPVISTPIGGIPEMVVDG
                     LTGILVTPGDSTALADAVEKLIVDLPLARRLGQSGYERARELFAIEKSAAALIRLFEQ
                     LRL"
     gene            1571..1873
                     /locus_tag="VFO30_05775"
     CDS             1571..1873
                     /locus_tag="VFO30_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418831.1"
                     /translation="MLERITRATAAVLLISMVLPGCSMLTARGRQERAYERYVRKCSH
                     QRDRLQAKMMKTPRIPTFEPSEPKETTEVGGSPESVTSSESATSPANAATSAETSN"
     gene            complement(1870..2247)
                     /locus_tag="VFO30_05780"
     CDS             complement(1870..2247)
                     /locus_tag="VFO30_05780"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9418832.1"
                     /translation="MSEQPQGELVIQTIAMPKDTNPSGDIFGGWLMSQMDLGSSILAA
                     KTAKARVATVAMEAMSFLHPVRVGDRVACYARIEKIGRTSMRIPVEVWVTRYQTGEQV
                     KVTHGVFTYVALGVDGKPIPVER"
     gene            complement(2271..3251)
                     /gene="queA"
                     /locus_tag="VFO30_05785"
     CDS             complement(2271..3251)
                     /gene="queA"
                     /locus_tag="VFO30_05785"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET9418833.1"
                     /translation="MSTRLSDYDYPLPNELIASRPLPDRDASRMMVLRRESQMIEHRQ
                     FRELKMLLEPGDLLVLNDTRVLPARRFSHDHAIEFLFVERLGSRRWKCLVKPGRKMRV
                     GASTHIDNVRLCVEEVLSEGERIVSLEEDVDLHAGGSMPLPPYIGRESDSEDNTRYQT
                     VFAQQPGALAAPTAGLHFTPEILRELPYTLVTLHVGPGTFRPVQSEDLRQHRMHPEWF
                     CISDEAARNIDNAKRVVAIGTTTVRVLESARREGGKLVAQTGSTDLFIYPPYRFEHVD
                     LLFTNFHLPRSTLLMLVSAFAGREFLLHAYTEAIRERYRFYSYGDCMLII"
     gene            3331..>3795
                     /locus_tag="VFO30_05790"
     CDS             3331..>3795
                     /locus_tag="VFO30_05790"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9418834.1"
                     /translation="MQFPSALDLVVRALEQIRETSDRAPCASRAAVAQLFAPERRMLS
                     NPKASGADSAAPSRTSKIDMLNALGARVAACTKCPHLVRTRTQTVFGVGNPNAELMFI
                     GEAPGVEEDAQGEPFVGRAGQLLTRIIRTMGLAREEVYIANILKCRPDMPAGS"
BASE COUNT          827 a         1172 c         1075 g          721 t
ORIGIN      
        1 gcgcggcgtt ccaattcgcc gacgcgaacg gtcgcaagat cgacatcgaa ttgcgtggga
       61 atgacggacc gccgattggc aagatcgtaa taaactcaac cgaagccgaa ttcagcgtcg
      121 cgcccgcgca atgcggcgac ctactggaaa tctcacgcgg ccgacctaac gaacagcgcg
      181 ccggccagct catgcccgcg caaaactacg atctcgccga tctgatgacg caggagttat
      241 tgcgcgcagg accgcatcac atttacctgc gcgccatcaa ttgcgtgcgc gatcttctct
      301 agccactgcg ccgtcagccc agacgggcga cacgcccgcg gctacaaacg caattgctcg
      361 aagaggcgaa tcaacgctgc tgcgctcttt tcaatcgcaa acaactcccg cgcccgctcg
      421 tatccgctct ggcccaaacg ccgcgcgagc ggaagatcga caatcagctt ctcgaccgcg
      481 tcggcgaggg ccgttgaatc acctggcgtg acgagaatac cggtcaggcc atccaccacc
      541 atctcgggga ttccgccgat tggcgtcgag atcaccggca gcccggccgc catcgcttcc
      601 atgatgacgc tcggcaaatt gtccatcccg ccttgcgcat cgattacgct cggcagagcg
      661 aacaccgctg ccgcggccaa ataaccaaca atgtcgcgtt gcggcttcgg cccaagcagc
      721 tcgacatggt tctgcaactg aaattctgcg attcggccac gcaggtcaga ttccagcggt
      781 ccttcgccga tgatttggca gcgaaagacg tggccacgtt ctttcagcaa ccggcagctc
      841 tcaagtagat aagggaagcc cttcttctca atgagacgcc caacgctgac aattgtcgga
      901 agttcggcgg aaaaatccgc gcgccggaat tcgaccaaaa ccagtccgtt gtagacgcgg
      961 tgaatccgct cgccacgctc ggcaaagcgc tctcgcagga aattcgcggc gtaatcggtt
     1021 tcagtcacaa caaaccgggc acgatcgacg agcttttcga ggctgatctc gaacgcgcgc
     1081 ggcgcgaaga tgtcattggc gtgcgccgtg aaactgaagc cgatcccaaa gaagttctcg
     1141 atccaatacg cggtgcgggc cgccatgccg gcgaaatgcg cgtgcacatg gcggataccg
     1201 gcatcttgca aaggcaagcc gacataaacc gcctgttgga gccggagaaa atcagtcttg
     1261 cgtccccagt gatcaatcgt ttcgcgaacc ggcggcagct ttcgcttacg cagcatccga
     1321 gcaacttctt cgagcaacgg tttctcttca ggcaggtaat ggacgcgctc cacgatctgc
     1381 tcgtcccaat cctgcgccgg ttcctccgtc ggccggcgca atgagtagat gtcgatctta
     1441 gcgccctgcc gaatcagctc cgcgacttcg cgatagcaaa acgtttgcga aaacgcgggg
     1501 aaccgctcga acagatatgc caaccgcatt gccgcgcgat tttcgcacga cttctgctct
     1561 agagaaaagg atgttggaac ggattaccag agccacggcc gcggttctgc tcatatcgat
     1621 ggtcctgccg ggctgttcga tgttgaccgc gcgcgggcgt caggagcgcg cttacgagcg
     1681 atacgtgcgc aagtgcagtc accagcgcga tcgtttgcag gcaaagatga tgaaaacgcc
     1741 gcgcatcccg acatttgagc catcggagcc gaaagagacg accgaagtcg gcggctcgcc
     1801 tgaatcggtc acctcgagcg aatccgcgac atcgccggct aacgccgcga catctgccga
     1861 aacttcgaac taacgctcaa ccgggatcgg ttttccgtct acgccgagcg ccacgtaggt
     1921 gaacacaccg tgcgtcacct tgacctgctc gcccgtttga taacgcgtca cccagacttc
     1981 cactggaatg cgcatcgacg ttcggccgat cttctcgatg cgggcgtagc aagcgacacg
     2041 gtcccccacg cgcaccggat ggagaaacga catcgcttcc atggcgacgg tcgcgacgcg
     2101 cgctttcgcc gttttcgcgg cgagaatact gctgcctagg tccatctgcg acatcaacca
     2161 accgccgaaa atatcgccgc tcgggttagt gtccttcggc atcgcgattg tctggataac
     2221 aagctcgccc tgcggttgct ccgacatgcc gccgagaata gcacaagcgg tcagatgatc
     2281 agcatgcaat cgccgtaact gtaaaagcgg tagcgctcgc gaatcgcttc tgtataggca
     2341 tggagcaaaa attcgcgacc agcgaacgcg ctcacgagca tgagcaaggt cgagcgcggc
     2401 agatgaaaat tcgtgaacaa aagatcgaca tgttcgaatc ggtaaggcgg ataaatgaac
     2461 agatcggtcg agccggtctg cgcaaccagt tttccacctt cgcgccgggc tgattccaaa
     2521 acgcgtaccg ttgtcgtgcc gatcgcgacg acccgcttcg cgttgtcgat attgcgcgcg
     2581 gcttcatccg agatgcagaa ccattccggg tgcattcgat gctgcctcag atcctccgat
     2641 tgcaccggcc gaaatgtgcc cgggccaaca tgcagcgtga ctaacgtgta gggcagctcg
     2701 cgtagaatct ccggcgtaaa atgaagtccg gccgtcggcg ccgctaaagc gccgggctgc
     2761 tgagcaaaca ctgtttggta gcgggtgttg tcttccgagt cgctctcacg tccgatgtag
     2821 ggcggcagcg gcatcgaccc gccagcatga agatcgacat cttcttcgag agagacgatc
     2881 cgctctccct cggacaaaac ctcctctacg cacaagcgca cattgtcgat gtgcgtgctc
     2941 gcgccgacgc gcattttgcg gccgggtttg accaggcatt tccatcgtcg ggacccgagc
     3001 cgttcgacga acagaaattc gatcgcgtgg tcgtgtgaaa atcttcgcgc tggaagcact
     3061 cgtgtgtcat tgaggacgag caaatctcca ggttccagca acatttttaa ttcgcggaac
     3121 tgtcgatgtt cgatcatctg cgactcgcgg cgcaacacca tcatgcgcga cgcatcgcgg
     3181 tcgggcagtg gccggcttgc gatcagctcg ttcggcaacg ggtagtcgta atcgctcaat
     3241 cgcgtgctca tccggtgcgc taaaccttac gtgtcgagtc gacgcgacaa aaagaattgc
     3301 gccgcgagaa aaaacttctc atgatccgac atgcagttcc cctctgcgct cgatttggtc
     3361 gtaagagcgc tggagcagat tcgggaaaca tctgatcgcg cgccgtgcgc gtcccgtgct
     3421 gcggtggcac aactgtttgc tccggagcga cgcatgctgt cgaaccccaa ggcttccggc
     3481 gccgacagcg cggcgccctc cagaacaagc aagatcgaca tgttgaatgc gctgggcgcg
     3541 cgcgttgccg cctgcacaaa gtgtccgcat ctcgtgcgca cgcgcacgca gaccgtgttt
     3601 ggggtgggaa atccgaatgc ggaactcatg tttatcggcg aagcgccggg cgtggaagaa
     3661 gacgcccagg gcgaaccctt cgtcggccgc gccggtcaat tactcacccg catcatccga
     3721 acgatggggc tcgcgcgcga ggaggtttat atcgcgaaca ttctcaaatg ccgaccggat
     3781 atgccggcag gttca
//
LOCUS       DASTDG010000178         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1395705, whole genome shotgun sequence.
ACCESSION   DASTDG010000178 DASTDG010000000
VERSION     DASTDG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395705"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="tgt"
                     /locus_tag="VFO30_05795"
     CDS             <1..830
                     /gene="tgt"
                     /locus_tag="VFO30_05795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9418835.1"
                     /translation="EDGVHFQNHVDGTAAFISPEIAMEIQATLGSDIAMLLDECPPWP
                     CERGYAARSAEMTTRWAKRCKENAERPTLKVQSRIQQLIFGVVQGATFDDLRKESAQA
                     IVDLEFDGYAIGGVSVGEPEEEMFRAVEASEPILPSDKPRYAMGLGTPPQLLEMIARG
                     MDMFDCVLPTRLARNGTAFTSTGTMNLKNAEFAMDKRPIEENCACPTCREFTRGYIRH
                     LVKAEEILGLRLITLHNLHFYLDLMRLAREKIDNETFDGFRRDFIANYQPRDVDVAL"
     gene            943..1266
                     /gene="yajC"
                     /locus_tag="VFO30_05800"
     CDS             943..1266
                     /gene="yajC"
                     /locus_tag="VFO30_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9418836.1"
                     /translation="MFALFLAQAQQGTPAPAPGGGIGFFVPLIFIFVIMYFVMIRPQK
                     RRQMEQQKLVSALKTGDRVVTNAGIHGLISNVKETTVIVKVADNVKIEMEKSAVTNVL
                     KASES"
     gene            1297..3651
                     /gene="secD"
                     /locus_tag="VFO30_05805"
     CDS             1297..3651
                     /gene="secD"
                     /locus_tag="VFO30_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9418837.1"
                     /translation="MSHPAIQFFIGLGVLVLFGWYFASDFGPRKRILAMTLVGLLIAL
                     SIETIWPPQKKIALGLDIQGGTSFLIRMMKGDKEITKGMQDQAVEVIRKRVDYFGGGE
                     PIISPVGDDRILVQIPGLDTAKIQEAREQLQKVAKLEFRLVHPQSDALISQIEAGTAV
                     TPPGYSIETLSEEREGKTIQKKLLIKKKADLSGNHVTHAAAGFGQEGWEVDLQFDSAG
                     AEQFGKLTEQVYNEHSAMAIMLDNKVISAPGVRNGPIYGGRAQISGGNMGEREARGLA
                     SVLENPLQTPVSIEEERSVSPTLGQDSIRASIIAGLVGLAITLVCVSIYYKFAGLIAN
                     LALIINLILLIGALTMFRFVLTLPGIAGIILTIGLSVDASVLIYERLREEMALGKSLK
                     VAVQTAYDKAFSSIFDANVTTLITAAILFWKASGPIKGFAISLTLGILASLFTALIVG
                     RNALSWFVDTGKLKRISMLHLISSQNFNFLGKGFLACMCSLALILAGATSFYLRGERN
                     FGVDFRGGDLVTLSSPNKIDIGQVRDGLKPLGLADASIQESAQGGKNYITIRSPLHTS
                     DKVQAQVMQAMPAANFHVEGSQRVGALVGGELAKSSLVALGLGILGILIFVTLRFELS
                     FAVGAIVALLHDVIITVGMFSLLHRELTLTMVGAVLTIAGYSINDTIVVYDRIREGLA
                     SGRRGTIEEIMNSSINQTLSRTILTSTVTLIPILCLFLFGGAVLRDFSLAIIIGVVVG
                     TYSSIFIASPIVLWWSRARGETTSSLRREITQKATTVNPALGSR"
     gene            3733..>3821
                     /gene="rpsU"
                     /locus_tag="VFO30_05810"
     CDS             3733..>3821
                     /gene="rpsU"
                     /locus_tag="VFO30_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9418838.1"
                     /translation="MPEVIVRKGEPVDRALKRLKSKLEAEGIL"
BASE COUNT          807 a         1064 c         1160 g          790 t
ORIGIN      
        1 ccgaagacgg cgtgcatttt cagaatcatg tcgatggaac ggcggcgttt atttcgccgg
       61 agatagcgat ggaaatccag gcaacgctcg ggagcgatat cgcaatgctg ctggatgaat
      121 gtccgccatg gccatgcgag cgcggttacg cggcgcgtag tgcggaaatg accacgcgat
      181 gggcgaagag atgcaaagaa aacgccgaac gcccaacgct caaggtccaa tctcgaattc
      241 agcagttgat cttcggggtc gtccaaggcg caacgttcga cgatctgcgc aaagagtcgg
      301 cgcaagcgat tgtcgatctt gaattcgatg gttatgcgat tggcggcgtg agtgtgggcg
      361 agccggaaga ggagatgttt cgcgcggtcg aagcgagcga gcccattttg cctagcgaca
      421 agccgcgtta cgcgatgggc ctcgggacgc cgccgcagtt gctcgagatg atcgcccgcg
      481 ggatggacat gttcgattgc gtcttgccga cgcggctcgc gcgaaatggg acggcattca
      541 cctcaaccgg cacaatgaat ttgaagaatg cggagttcgc tatggacaag cggccgatcg
      601 aagagaactg cgcgtgcccg acatgccgcg aattcacccg cggttatatt cgccatctcg
      661 tcaaagcgga ggaaattctc ggactgcgcc tgataacgct ccacaatctg catttctatc
      721 tcgacctgat gcggctggcg cgggagaaga tcgacaatga aacattcgac ggcttccggc
      781 gcgatttcat tgcgaactac cagccgcgag atgtcgatgt tgctctgtag ccacgtgccg
      841 gtgggacgtt ctgttccggc ggcgcacagc gccgtggcta cagcgattct ggattgcgag
      901 cagcgcattc gcgcttaagc tggcgggcgc tttctcttcg gaatgtttgc tttgttttta
      961 gctcaagcgc agcaagggac gcccgctcca gcaccgggcg gcggcattgg gtttttcgtg
     1021 cccctgattt tcattttcgt catcatgtat tttgtgatga tccggccgca gaaacggcgg
     1081 cagatggagc aacagaagtt agtctcggca ctgaaaacag gcgaccgggt cgtgacgaac
     1141 gcgggcattc acggcctgat ctcgaacgta aaggagacaa ccgtcatagt aaaagtcgcc
     1201 gacaacgtga agatcgagat ggagaaatca gcggtcacca acgtgctgaa agcatccgaa
     1261 agttgaagtg agacccgtca aaacgttgga gcggacatga gccacccggc aatccagttc
     1321 ttcattgggc ttggcgttct cgtgctgttc ggttggtatt ttgcgagcga tttcgggccg
     1381 cggaaacgca tcctcgcgat gacactcgtc gggttgctga tcgcgctgag catcgagacg
     1441 atctggccgc cgcaaaagaa gatcgcgctc ggcctcgata ttcagggcgg cacttccttc
     1501 ctcatccgga tgatgaaggg cgacaaggag atcaccaaag gaatgcagga ccaggccgtg
     1561 gaggtcatcc gcaaacgggt cgattacttc ggcggcggag agccgattat cagccccgtc
     1621 ggtgacgacc gcatcctcgt tcagattcct gggctcgaca cggcgaagat tcaggaggca
     1681 cgggagcaat tgcagaaggt cgcaaagctc gagtttcgac tggttcatcc acaaagcgac
     1741 gcgctgatct cgcaaatcga agccggaaca gccgtcacgc ctccggggta ttcgatcgaa
     1801 acgctttccg aagaacgcga agggaaaaca attcagaaaa agcttttgat taagaagaag
     1861 gcggatttgt cagggaacca tgtgacacac gcggcggccg gttttggtca ggaaggctgg
     1921 gaagtggatc tgcaatttga ttccgctggc gccgagcagt tcggtaaact gacggaacag
     1981 gtttacaacg agcattccgc tatggcgatc atgctcgaca acaaagtgat ctcggcgcca
     2041 ggagtgagga acgggccgat ctacggcggc agagcccaga tcagcggggg taatatggga
     2101 gaacgtgaag cacgtggcct cgcgagcgtg ctggagaacc cgttgcagac gccggtcagc
     2161 atcgaggaag agcgcagcgt ctccccaacc cttggtcagg actcgatccg ggcgagtatc
     2221 atcgccgggt tagtcggcct cgcgatcacg ctcgtttgcg tgtcgatcta ctacaaattc
     2281 gccggcttga tcgcgaacct cgccctcatc atcaacctga tcctgctgat tggcgcgctg
     2341 acgatgttcc ggttcgtgct gacgctgccg ggcatcgctg gcatcatttt gaccatcggt
     2401 ctctcggtcg acgcgagcgt gctgatttac gagcggttgc gggaggagat ggccctcggc
     2461 aaatcgctca aggtggcggt gcagactgct tacgataaag cgttcagctc cattttcgac
     2521 gcgaacgtga cgacgctgat cacggcggcg attttgttct ggaaagccag cggcccgatc
     2581 aaaggcttcg cgatttcgct cacgctcggc attctggcgt cgctgttcac cgcgctcatc
     2641 gtcgggcgca acgcgctgag ctggttcgtc gataccggga agttgaagcg catttcgatg
     2701 ttgcatctca tttcatcaca gaacttcaac ttcctgggca aaggtttcct cgcctgcatg
     2761 tgctccctcg cgctcatcct cgcgggcgcc acttcgtttt atctccgcgg cgagagaaat
     2821 ttcggcgttg atttccgcgg tggcgatctg gtgacgctca gctcacctaa caagatcgac
     2881 atcggccaag tgcgcgatgg gctgaagccg ctcggcctgg ccgacgcctc gatccaggaa
     2941 tcggcgcaag gcggcaagaa ctacatcacg atccgaagcc cgctgcacac gagcgacaag
     3001 gtgcaggcgc aagtgatgca agcgatgcca gctgccaact tccatgttga aggctcgcag
     3061 cgcgtcggcg cgttggtcgg cggcgagttg gcgaaaagtt cactcgtcgc gctggggctc
     3121 ggcattctcg gcatcctgat cttcgtcacg ttgcgctttg aattgtcctt tgcggtcggc
     3181 gcgatcgtcg cgctattgca cgacgtgatc atcaccgtcg ggatgttctc gcttctacac
     3241 cgcgagctga cgctcacgat ggtcggtgcc gtccttacga tcgccggtta ctcgatcaat
     3301 gacacgatcg tcgtgtacga ccggattcgt gaaggtctcg ccagcggccg acgcggcacg
     3361 atcgaagaga tcatgaactc gagcattaac cagacgctca gccgcacaat acttactagc
     3421 acggtgacgt tgatcccgat cctgtgcctg ttcctgttcg gcggcgccgt gctgcgcgat
     3481 ttctccctcg ccatcatcat cggcgttgtc gtaggcacct actcgtccat cttcatcgcc
     3541 tcgccgatcg tgctctggtg gagccgcgcc cgcggcgaaa ccaccagttc gctgcgtcgc
     3601 gaaatcacac agaaggcgac gaccgttaac ccggcgctcg gaagtcgcta ggcacaggct
     3661 cagttgaccg gcagagcgct cccggctaga ctcggctctc gggtcgcagg agatgaggag
     3721 ctgatttatc ccatgccaga agtgatcgtt cgcaaaggtg aaccggtgga tcgcgctttg
     3781 aaacggctca agagcaagct cgaagcagaa gggattctcg a
//
LOCUS       DASTDG010000179         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1594397, whole genome shotgun sequence.
ACCESSION   DASTDG010000179 DASTDG010000000
VERSION     DASTDG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594397"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..742)
                     /locus_tag="VFO30_05815"
     CDS             complement(260..742)
                     /locus_tag="VFO30_05815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9418839.1"
                     /translation="MSNVALVVIAVFTLAAALGAATNPNLMHAALSFALAFVGVAAFY
                     FLLGAEFVGLVQVFVYIGAVAVLVVFTILLTRREGEKHRGFNWGGVLVAIAMFFGLAW
                     AVVKTPSLSILPPDIEPLTVKRIGEVLMTDYPWPLMGAGLLLTVALLGALILAMEEKR
                     "
     gene            complement(739..1929)
                     /gene="nuoH"
                     /locus_tag="VFO30_05820"
     CDS             complement(739..1929)
                     /gene="nuoH"
                     /locus_tag="VFO30_05820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9418840.1"
                     /translation="MADSVDQIFIRAKHLLLTPIIGRAPDWLVQIASSLLNIFALLGV
                     FLTLFALISVLERKILGRMQNRYGPNRVGPFGLFQPIADGIKMLIKEDVVPARADKIV
                     HFLAPILIAATAILALGVIPYGRNMTPFSIDGGILFFFAVGSTTELAVFMAGWGSNNK
                     FSMLGAMRAIAQMISYELPLVITVLPVVMIVGALNADAIVAAQAGYKIDIIPRWFVLT
                     PWGATAFILFFVSGLVESNRTPFDVPEGESEIVAGHMTEYSGFKYATFFMAEYIGMFA
                     ISGLGVTLFLGGWHAPIRFLEFVPSYVWFFLKLSVVLFVYIWLRGTLPRTRVDQIMNF
                     AWKFMLPMSFTCVIAAAVWHYAGRGLRGWLWSFVVVASVYTALSMLLDTRRKFAPRVY
                     RFAE"
     gene            2041..2415
                     /gene="ndhC"
                     /locus_tag="VFO30_05825"
     CDS             2041..2415
                     /gene="ndhC"
                     /locus_tag="VFO30_05825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9418841.1"
                     /translation="MTPNPYLFIVIFVCVALIFPLLPLGLAWLWRKLFQPPKPGPAKL
                     SIYECGVESIGEAQIQFRSQYYLYAIIFLIFDVEAVFLVPFAVAFTGLPVGAFIAILI
                     FLLLLIEGLVWAWGKGCLQWER"
     gene            2441..3064
                     /locus_tag="VFO30_05830"
     CDS             2441..3064
                     /locus_tag="VFO30_05830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HET9418842.1"
                     /translation="MSEEIDEGLRTELQRQGVWVTSMQELYNWGRKNSIWPLQFGLAC
                     CAIEMIATAASRYDIARFGGEIFRPSPRQADLMIVAGTVTKKMAPQIVRLYNQMPDPK
                     YVIAMGACAISGGPFKQGYNVLKGIDRYIPVDVYIPGCPPRPEALLHAFMELQRKIDS
                     QKLSGTEKAAHLDPEQPSEFPVPTFGKHDLEPPANPELFHPPRIERA"
BASE COUNT          762 a          857 c          946 g          631 t
ORIGIN      
        1 ctgcggattg aaccgccaga acgctatgaa attgatattg gcggcggcga gcatcagctc
       61 gatgccgatc aaaatgaaaa tggcgtggcg gcgactgagc gcggcgagca gcccaatcga
      121 gaacaacgcg acggaagcaa ggagaaacaa ttggagcgtt ggtgtcatgg cggatcagtt
      181 gcaaacgtta caagagtgac aatggttccg agttcgcctc ttctgtagaa tgtaaccttg
      241 agggaccttt taaccttttt caccgcttct cctccatcgc gagaatgagc gcgcctaaca
      301 aggcgacggt gagcaataaa cctgcgccca taagcggcca ggggtagtcc gtcatcaaca
      361 cttcgccgat acgtttcacc gtgagtggct caatgtccgg cggcaagatc gagagcgacg
      421 gcgttttcac aactgcccat gccaagccga aaaacattgc gatggcgacg agcacgccgc
      481 cccagttgaa gccgcgatgt ttttcgcctt cgcgccgcgt gagcagaatt gtgaacacaa
      541 ccaagacggc gacggcaccg atgtagacga agacctgcac gagcccaaca aattcagcgc
      601 cgagcagaaa atagaacgcc gcgacgccga cgaatgcgag ggcaaaactg agcgcggcat
      661 gcatgaggtt ggggttggtc gcggctccga gggcggcggc cagggtaaag acggcgatca
      721 ccactaacgc gacgttgctc attcggcgaa gcgatagacg cgcggcgcga atttccgccg
      781 cgtgtcgagc aacatcgaca atgccgtgta gacgctggcg acgacgacaa acgaccacag
      841 ccagccgcgc aatccgcgcc cagcataatg ccacacggct gcggcgatca cacacgtgaa
      901 ggacatcggc aacatgaatt tccaggcgaa attcatgatc tgatcgacgc gggtgcgcgg
      961 cagcgtgccg cgcagccaga tgtaaacaaa gagcacgacc gatagcttga gaaaaaacca
     1021 gacgtaggat ggaacgaatt ccagaaaacg tatcggcgcg tgccagccgc cgaggaacag
     1081 tgtcacgccg aggccgctga tcgcgaacat gccgatgtat tcggccatga aaaatgtcgc
     1141 gtatttgaac ccggaatatt cggtcatgtg gccggcgacg atttccgatt cgccttcggg
     1201 cacgtcgaac ggcgtccggt tggattcgac caagccggag acaaaaaata aaatgaaggc
     1261 ggttgcgccc caaggggtga gcacaaacca acgcggtatg atgtcgatct tgtaaccggc
     1321 ctgtgcggcc acaatcgcgt cagcgttcag cgcgccgaca atcatgacca ctggcagcac
     1381 ggtgatgacc agcggcagct cgtagctgat catctgcgcg atggcgcgca tcgcaccgag
     1441 catggagaac ttgttgttac tcccccaccc ggccatgaag acggccagct cagtcgtcga
     1501 tccgacggca aagaagaaca gtatcccgcc atcgatcgag aacggcgtca tgttgcgtcc
     1561 gtacggaatc acgccaagcg caaggatcgc ggtggcggca attagaatcg gcgcgagaaa
     1621 gtgaacgatc ttgtcggcgc gcgccgggac gacgtcttcc ttgatcaaca ttttgattcc
     1681 gtcggcaatc ggttggaaca ggccgaaggg accaacgcga tttgggccat agcgattttg
     1741 catgcgtcca agaattttcc gctcgagcac ggaaatgagc gcaaacagtg tcaggaaaac
     1801 tccgagcagc gcgaagatgt tgagaaggct cgaggcgatc tgcacgagcc agtcgggcgc
     1861 gcgcccgata atcggcgtaa ggaggaggtg ctttgcgcgg atgaaaatct gatcaactga
     1921 atcagccatt tggatcgcca cggattaaca cagatgaaac acagatgaaa gggtagtatt
     1981 ctttctgtct actggtgtga gatctctatt cctctgcgac tgattagttg ttttgtgaga
     2041 atgactccga atccttatct gtttatcgta atttttgttt gcgtggcgct gatctttccg
     2101 ctgctgccgc tcggcctggc gtggttgtgg cggaaattgt ttcagccgcc caagcccggg
     2161 cctgcgaaat tgtcgatcta cgagtgcggc gtggagtcga tcggtgaagc gcagattcaa
     2221 ttccgatcgc aatattatct ctacgcgatc atctttttga tcttcgatgt cgaggcagtg
     2281 tttctggtgc cgttcgcggt ggcattcacc gggttgccgg ttggcgcgtt catcgccatt
     2341 ctcatctttc tgctgttgct gatcgaagga ttagtttggg cgtggggcaa aggttgctta
     2401 cagtgggaac gttgaagggt tgaagcgtca aagcgaagcg atgagcgaag aaatcgacga
     2461 ggggctgcga accgaattgc agcgacaggg tgtctgggtc acgtcgatgc aggagcttta
     2521 caattggggt cgcaagaatt cgatctggcc gctccagttt gggctggcgt gctgcgccat
     2581 cgagatgatc gcgactgcgg cgtcacgtta cgacattgcg cgcttcggcg gcgagatttt
     2641 ccggccgtcg ccgcgtcagg ccgacctgat gattgtcgcc ggcacagtga cgaagaagat
     2701 ggcgccacaa atcgtcaggc tgtacaacca gatgccggac ccaaagtacg tcatcgcgat
     2761 gggcgcatgc gcgatctccg gcgggccgtt taagcaaggc tacaacgtcc tcaaaggaat
     2821 cgaccgctac attccagtgg acgtttacat tcccggttgt ccaccgcgcc ccgaagcatt
     2881 gctgcacgcg ttcatggaac tgcagcgcaa aatcgatagt caaaaactga gcggcaccga
     2941 gaaggccgca catcttgatc ctgaacaacc gagcgaattt cccgttccaa cgtttggcaa
     3001 gcacgatctt gagccgccgg cgaacccgga gctttttcat ccaccgagaa ttgaaagagc
     3061 gtgaagctcc ggaacataga ctttcagtct gtgcgcccag cggagttcga ctccgctgat
     3121 tttcagaaca gcgggcagaa tgcccgctgg gcgcacaggc cgcaggccta tgttccgact
     3181 cgataatgga atcgct
//
LOCUS       DASTDG010000180         5501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_520525, whole genome shotgun sequence.
ACCESSION   DASTDG010000180 DASTDG010000000
VERSION     DASTDG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520525"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFO30_05835"
     CDS             complement(<1..966)
                     /locus_tag="VFO30_05835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9418843.1"
                     /translation="MMAWTIYGTFAGAVAVLLLPRPFSRWIALLTTLAGFAIALLALI
                     RTPIVDLAQFTTIVRVAWVPALGMNYHLSIDGISLTLVLVTGIVAVAAVLFSWDVGSR
                     SNEFFFWLLLVVGGSYGVFLSADLFLLFVFYELVIVPKYFLIAIFGSTNKEYGAMKLT
                     LYSFFGGTLVFIGIIAAYVTGGSLDLNQLAQFSFPMQLQAWAFPVLFLGFAVLAGIWP
                     LHTWAPTGHVAAPTAGSMLLAGIVMKLGAYGGLRVAMNLFPQGFQMWRNCIVVLAVIG
                     IVYAAAVALRQSDLKFVIGYSSVSHMGFVLLGLATANALGVSGAVL"
     gene            complement(963..1514)
                     /locus_tag="VFO30_05840"
     CDS             complement(963..1514)
                     /locus_tag="VFO30_05840"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HET9418844.1"
                     /translation="MSKPIAPLEKIARTILVLRGERVLLDADLARLYGVTTGNLNKAV
                     QRNRDRFPADFMFQLTGAEADLIFQFGRSKGRGGRRHFPFAFTEQGVAMLSSVLNSER
                     AVKVNIAIMRAFVKLREALETNRELAKRFAELETRIGKHDEEISAIIEAIRQLMSPPE
                     KSRREIGFHVREKSAVYRTRDGR"
     gene            complement(1518..3002)
                     /locus_tag="VFO30_05845"
     CDS             complement(1518..3002)
                     /locus_tag="VFO30_05845"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9418845.1"
                     /translation="MAGLISAFIVVPLIGAIAVYLLSAKHARVVALLVTAIAAVYAFL
                     LWRGFSASASGLQLVERHTWIPSIGAEYLVGIDGLSLLLLLLTTLLFPFALLAQRLER
                     GACALMLLMQSALYGVFVAQNFVLWFLFYEMTLVPAFLLIKIYGGENRDRAAVKFFLY
                     TFLGSIMLLLAFLGIYFARGTFDFGQLVDLAKGGPLVIGKFAWLIFAGIFIGLAVKVP
                     LFPLHTWLPDAYETAPTGVSMVLTGALSKMGVYGFIRLLVPLFPNEIRVVGPWLLALV
                     VCSILVGALVAWAQSDLKRMVAYLSINHLGYCLLGLFAIASARSASSLIDMHAALSGV
                     FLQIFNHGITAAALFYFVGLLEQRRGARGLGDFGGLMQSAPLLCGWMSVAMFSSLGLP
                     GLNGFIGEFLIFKGSFAVAPPFTALAVLGLLFTAVTFMRAMQALFSGPAPDATNAIVD
                     LTRSEKFVVVPVTLLMFAIGLAPQFIFTIFNSTVVQMARLFALS"
     gene            complement(2990..4963)
                     /gene="nuoL"
                     /locus_tag="VFO30_05850"
     CDS             complement(2990..4963)
                     /gene="nuoL"
                     /locus_tag="VFO30_05850"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9418846.1"
                     /translation="MNSWIVSNLPLIPGVPLAASLLILRLPNSWRKTAAAFAVIGQMA
                     ALALSIFGFLATLQTPGFRAVQNFTWFTFGSQALQLGFVLDPLGAAMLLMISLVGLCI
                     FVFSIGYMADDKHFTRFFAYLSFFSGAMLGVVTANSLLLLFIFWELVGLASYLLIGFW
                     IERPSAAAAAKKAFVTTRIGDMGLFLGILWLYRSSGTLLFYDSGRGCLEPAALALLGA
                     SATSIALLIFCGAVGKSGQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVARVYP
                     IFNVGAINGVTPSLTVVVWIGVITGLMAALIAVAQFDIKRILAYSTVSQLGLMMVSLG
                     VGGVAAGIMHLLAHGFFKALLFLGAGSVIHGLHHEQDIRKMGGLRRAMPITFATYAVG
                     MMALSGLPLFFCGGWTKEEILFATSQWPRSHAPYLLLMVGVVLTALYMTRQVIYIFFG
                     NRAPEAHESPRVMTVPLVVLAICTIGLSIVLTPAWPWLDRYLLGESAHVDVAKLFQPM
                     MLTSLLLVAIGIGLGAFFYRTIVQDRHRDPLEHAQPALFSFLANRMWLDELYDKTVIL
                     CSRTAARVGDWLDQNVWDGLVRGIGALGQAFGIFTTTMDERGINAGVDETTTGARGLG
                     RVMSAAHSGQIQAYLGAIGLGMLALLLLYAWLG"
     gene            complement(5089..5400)
                     /gene="nuoK"
                     /locus_tag="VFO30_05855"
     CDS             complement(5089..5400)
                     /gene="nuoK"
                     /locus_tag="VFO30_05855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9418847.1"
                     /translation="MTPTLQLFLLASVALFSIGLLAALSRRHAIFILIGIELMLAAAN
                     INFIAFWRFNPQPQPIGVMVAIFAIAIAAAEAAVGLAMVIAVYRHYRTTNVDQLDQLK
                     G"
BASE COUNT         1398 a         1543 c         1652 g          908 t
ORIGIN      
        1 caggactgcg ccggaaacgc cgagtgcatt cgcggtggcc aggcctaaca agacaaaacc
       61 catgtggctc acgctcgaat agccgatgac aaatttcaga tcactctggc gcaacgcgac
      121 cgccgcggcg taaacaatcc caatcaccgc gaggacgacg atacaattac gccacatctg
      181 aaaaccttgc gggaaaagat tcatcgcgac gcgcaaacca ccgtaggcgc cgagcttcat
      241 cacgattccg gccagcaaca tcgagccggc ggtcggcgct gcgacatggc cagtcggcgc
      301 ccaggtatgc aacggccaga tgccggcaag cacggcgaaa ccgagaaaca agactggaaa
      361 ggcccaggct tgcagttgca tcggaaatga aaattgcgcg agctggttga gatcgagcga
      421 gccgccggtc acatacgccg cgatgatacc gataaaaacg agcgtgccac caaagaagga
      481 atagagcgtc agtttcatcg cgccgtattc cttgttcgtg gaaccgaaga tcgcgatgag
      541 gaaatacttc ggcacgatca caagctcgta gaagacgaag agaagaaaaa ggtcggcact
      601 taagaaaacg ccgtaagagc cgccgacgac gagcaacaac caaaagaaga attcgttaga
      661 ccgagagcca acatcccagg agaacaacac cgcagcgacg gcgacgatgc ctgtaacaag
      721 cacgagcgta agactgatcc cgtcgattga gagatggtaa ttcatgccga gcgccggcac
      781 ccaggcgacg cgcacgatgg ttgtgaactg agcaagatcg acaatcggag ttcggattaa
      841 tgcgagcagt gcgatggcaa agccggcgag cgtcgtgagc agtgcgatcc agcgcgaaaa
      901 agggcgcggc aacaacaaca ccgcgaccgc ccccgcaaag gtgccgtaaa tcgtccaggc
      961 catcatcggc cgtcgcgcgt gcgatacact gccgatttct cgcgcacgtg aaatccgatc
     1021 tctcgccgcg acttttccgg cggcgacatc aactgccgga tcgcctcgat gattgcactg
     1081 atctcctcat cgtgtttgcc gatgcgtgtt tcgagttcgg cgaatcgttt cgccaactcg
     1141 cgatttgtct cgagcgcctc gcgcagcttc acgaacgcgc gcatgatcgc gatgttcacc
     1201 ttcacagcgc gctcgctgtt cagcacgctc gaaagcatcg caacgccttg ctcggtgaac
     1261 gcaaaaggaa aatggcgccg accaccacga ccttttgatc ttccaaattg gaatatcaaa
     1321 tcagcttccg ccccggtgag ctggaacatg aaatctgccg gaaagcgatc gcggttgcgt
     1381 tgcaccgctt tattgagatt gccggtggtg acgccgtaga gccgcgccag gtcagcatcg
     1441 agcaggaccc tttcaccgcg gaggacaaga attgtgcgcg cgattttctc gaggggcgca
     1501 atcggcttgc tcatcgatca gcttaaggca aacaggcgcg ccatctgcac gaccgtgctg
     1561 ttgaagatcg tgaagataaa ttgcggcgcg agaccgatag cgaacatgag gagcgtcacc
     1621 ggcacgacga cgaatttttc actgcgcgta agatcgacaa tcgcatttgt cgcgtcagga
     1681 gcggggccgc tgaagagcgc ttgcatcgcg cgcatgaaag tcacggcggt gaagagcagg
     1741 cccagcacgg caagcgcggt gaatggcggc gcaaccgcaa acgagccttt gaagatgagg
     1801 aactcaccga tgaagccgtt tagtcccggc aggcctaacg aggagaacat cgccacgctc
     1861 atccagccac agagcaacgg cgctgattgc atgagtccgc cgaaatcacc aagcccgcgc
     1921 gcgccacggc gttgctcgag caaaccaacg aagtagaaca acgcggcggc cgtaatgccg
     1981 tggttgaaga tttggagaaa gacgccgctg agcgccgcgt gcatgtcgat caaagacgac
     2041 gccgatctcg ccgaggcgat ggcaaagagg ccgagcaggc agtagccaag gtgattgatg
     2101 gagaggtagg cgaccatgcg tttgagatcc gactgcgccc atgcgacgag cgcgcccacg
     2161 agaatcgagc acacgaccag cgcgagcagc catggtccaa caactctgat ttcatttggg
     2221 aatagcggca cgagaagccg gatgaatccg tagacaccca tctttgacaa cgcgccggtc
     2281 agcaccatcg acacgccggt cggcgcggtt tcgtaagcgt ctggcagcca ggtgtgcaac
     2341 gggaatagtg gaaccttgac ggcgaggcca atgaaaattc cggcgaatat gagccatgcg
     2401 aacttgccaa tgacgagagg tccgcctttg gcaagatcga caagctggcc aaagtcgaat
     2461 gtgccgcgtg cgaagtaaat cccaaggaac gcgagcagga gcatgatgct gccgaggaag
     2521 gtgtagagga aaaacttcac cgccgcgcgg tcgcggtttt ctccgccgta gattttgatc
     2581 agcagaaagg cgggaacgag cgtcatctcg taaaacagaa accagagaac aaaattttgc
     2641 gcgacgaaca cgccgtagag ggcggattgc atgagcaaca tgagcgcgca cgcgccgcgc
     2701 tccagtcgtt gcgcgagcaa cgcgaagggg aacagaagag ttgttagcaa aagcaggagc
     2761 agactcaggc catcgatgcc gacgagatac tcggcgccga tgctcggaat ccaggtgtga
     2821 cgctcaacga gttgcagacc gctggcgctc gcgctgaatc ctcgccacaa cagaaacgcg
     2881 taaacggcag cgattgccgt aacgagcagc gcgacgacgc gcgcgtgttt cgcactgagc
     2941 aagtaaaccg caattgcgcc gatcagcggg acgacgataa aggcgctgat taacccagcc
     3001 atgcgtaaag gaggagcaat gcgagcatgc cgaggccaat cgcgccgaga tacgcctgga
     3061 tttgtcccga gtgcgcggcg gacatcacac ggccgaggcc acgcgcaccg gtcgttgttt
     3121 cgtccacgcc ggcgttaatc ccacgctcgt ccatagtcgt ggtaaagatg ccgaaggcct
     3181 gcccaagagc gccgatgccg cgaacgagac cgtcccagac attttggtcg agccaatcgc
     3241 cgactcgcgc tgccgttcgg ctgcaaagga tgacagtctt gtcataaagc tcgtccagcc
     3301 acatgcgatt ggcgagaaag ctgaacagtg cgggctgcgc gtgttcgagc ggatcgcgat
     3361 gtcgatcttg cacgatggtg cggtagaaga aagcgccgag gccgatgcca attgcgacaa
     3421 gcaacaatga tgtgagcatc atcggttgaa acaacttcgc tacatcgaca tgggccgatt
     3481 cgcctaacaa atagcgatcg agccatggcc atgccggtgt gagtacgata ctcaatccga
     3541 tggtgcagat cgcgagcacg acgagaggaa cggtcatcac acgcggactt tcgtgcgcct
     3601 caggtgcccg gttgccaaaa aagatgtaga taacctgccg cgtcatgtag agcgccgtga
     3661 gaacgacgcc gaccatcagc agcaagtacg gcgcgtgcga tctcggccat tgcgaggtgg
     3721 caaacagaat ttcttctttt gtccagccgc cgcagaagaa cagcggtagg ccgctgagcg
     3781 ccatcatacc gacagcgtaa gtcgcgaacg tgatcggcat tgcgcggcgc agtccaccca
     3841 ttttgcggat gtcctgttcg tggtgcagac cgtgaatgac cgagccggcg ccaagaaaca
     3901 gaagcgcttt gaaaaagccg tgcgcgagca aatgcatgat gccggccgcg acaccgccga
     3961 cacctaacga caccatcatc aacccaagct gcgagaccgt cgagtaagcg aggatgcgtt
     4021 tgatgtcgaa ttgcgcgacg gcaatcagcg ccgccatgag tcccgtgatg actccgatcc
     4081 agacaaccac cgtcagcgac ggcgtcacgc cattgatcgc gccgacgttg aagatcggat
     4141 agacccgcgc gacgagaaac acgccggccg cgaccatcgt ggcggcatga atgagagcac
     4201 tgaccggcgt tgggccttcc atcgcgtctg gcaaccagac gtgaagcgga aattgcccgg
     4261 acttgccgac ggcgccgcag aagatgagca gcgcgatgga agttgcgctc gcgcctaaca
     4321 aagcaagcgc cgctggttca agacaaccac gcccgctatc atagaagagg agcgtgccgc
     4381 tgctgcgata cagccacagt atcccgagga acaatcccat gtcgccgatg cgggtggtga
     4441 cgaacgcttt tttggccgca gccgcggcgc tcggtcgctc gatccaaaaa ccgatgagca
     4501 gatacgacgc taacccgacc agttcccaaa agataaacag cagcagcagg ctgttggcgg
     4561 tgacgacgcc gagcatcgcg ccggagaaaa acgagaggta ggcaaagaag cgggtgaaat
     4621 gtttgtcgtc ggccatgtag ccgatgctga aaacgaagat gcacaggccg acgagggaga
     4681 tcatgagcag catggcggct ccgagcggat cgagcacgaa gccgagctgc agcgcctgac
     4741 tgccgaacgt gaaccaggtg aaattttgga cggcgcgaaa acctggcgtc tgcagcgtcg
     4801 cgagaaaccc gaagatcgac aatgcgaggg cagccatctg gccgatgacg gcgaatgcgg
     4861 ccgcggtttt ccgccacgaa ttcggcaaac gtaaaatcag gagcgatgcc gcaagcggca
     4921 cgccggggat cagcgggaga ttactgacga tccaggaatt catcaggcaa aaagcggaaa
     4981 cgccgaacgc tcaacgccca acgctgaatc tcgaactcaa agcgctttct gaattgagcg
     5041 ttggacgttg ggcgttgggg gttgggcgtt tgcgtcgtcg gtttcatgct accctttgag
     5101 ctggtcgagc tgatctacgt tggtcgtgcg atagtggcga taaacggcga tgaccatggc
     5161 gagaccgacg gccgcttccg cagcggcaat ggcaatggcg aaaatcgcca ccatcacgcc
     5221 gatcggttgt ggctgcggat tgaaccgcca gaacgctatg aaattgatat tggcggcggc
     5281 gagcatcagc tcgatgccga tcaaaatgaa aatggcgtga cggcgactga gcgcggcgag
     5341 cagcccaatc gagaacaacg cgacggaagc aaggagaaac aattgaagcg ttggtgtcat
     5401 ggcggatcag ttgcaaacgt tacaagagtt acaataggtc cgagttcgcc tcttctgtaa
     5461 tgatgtaacc gtgaggactt ttttaacctt tttcaccgct t
//
LOCUS       DASTDG010000181         4488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_242792, whole genome shotgun sequence.
ACCESSION   DASTDG010000181 DASTDG010000000
VERSION     DASTDG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242792"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="rmuC"
                     /locus_tag="VFO30_05860"
     CDS             <1..937
                     /gene="rmuC"
                     /locus_tag="VFO30_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET9418848.1"
                     /translation="KELQQDNAQQIERMRATVDEKLQGTLEKRLGESFKLVSERLEQV
                     HRGLGAMQQLASDVGGLQKVLANVKTRGGWGEVQLGALLEQVLTPDQFARNVQTRDET
                     SERVEYAIKLPGDENGAPVWLPIDAKFPTEDYHRLLAAQDKGDVDVVESAMKNLEVQL
                     KKSARDICAKYINPPRTTDFALMFLPTEGLYAEAIRRVGLVEQVQRDCRVVFAGPTTL
                     AALLNSLQMGFRTLAIQKRSSEVWNLLAAVKTEFGKFGEALSAVKDKLDQAARHVDNV
                     AVRSRAITKKLRDVEELPSNPQPLLKNLLGDSDEE"
     gene            complement(991..2808)
                     /locus_tag="VFO30_05865"
     CDS             complement(991..2808)
                     /locus_tag="VFO30_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET9418849.1"
                     /translation="MILPRRILALIALVVPLTSHFATVTPDAEYEAVAEEYIKGYFNA
                     RPLQGTALGLHEYDGKITDYSRLALDAELARLRRFDDRLKKFEPDKLSQRQSVDLRIL
                     QAAIKKELFQMQDMSIFERNPMVYARAADVNVYIKRNFAPLEDRVRSIIAIESQVPNI
                     MIAARTNLADVLPKPYVELAMQIARGAADFLKKNLVAAVADLKSEQVRGDFQETNRKA
                     AAALMDYAAWLEREKLPKASPDFAIGEEKFQRLLAETELVDLTPQQVLEIGMAQLKNE
                     QTAFAETARRIDPNKPALEVFKQIQAEHSTPENLLPEMGKDLDQIRQFVTKRHLVTIP
                     SEVRPRVKETPQYRRATSFASMDTPGPFEKRATEAYFYVTPTEPDWPNEQKEQWLTAF
                     NYYTADLISIHEVYPGHYVQFLRLNASPASKVEKIFGSYAFVEGWAHYCEQMMVDEGY
                     TGSRHDSGGSPTATPSPAAKASPSASPVDESRAAKYRMAQASEAMLRLCRLCAAIKMH
                     TQHMSIDDGTKFFQENCYYEEKPARAEAMRGTFDPGYFNYSLGKLQILKLRDDYKAQE
                     GDAFSLQKFHNELLNHGMPPIRLLRELMLKDKTHWDQVL"
     gene            2908..4209
                     /locus_tag="VFO30_05870"
     CDS             2908..4209
                     /locus_tag="VFO30_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9418850.1"
                     /translation="MRPCFRLGMLLVWTCVLALPPHGRAAQVTNPSEDDNGYSQISIF
                     AKAVQLLRQDYVDANKTSYHDLITAAMKGMLASLDPHSQFMDPEDFRDMQEDTRSRFN
                     GLGIEVSMKNGLPTVVTPMEDTPAAKAGILSGDQILKINGASTEKMELQDAISLLRGP
                     AGGKVTLTLLRPSTKDVKDYTLERTEIKVQSVKGARLLDAALTNPFKIGYVRLIQFNE
                     PTADELSKALDQLQKQGMQGLILDLRNNPGGLLNSAVDVCAQFLPPNTKVVSTEGRVP
                     SQQREYTTPATAKERPHFPMAVLANEGSASGAEIVCGAFKDLHRAVIIGETTFGKGSV
                     QNVMQLPDGSALRFTTAKYYTPSKQQIHGNGVTPNIRVPMSAEQERALFALRSGDSVK
                     PEEDKEIIKTRDPQMLRAIDALKGVMIYAEQNAGKPESVKK"
     gene            4283..>4488
                     /locus_tag="VFO30_05875"
     CDS             4283..>4488
                     /locus_tag="VFO30_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9418851.1"
                     /translation="MTILALETSCDETAVAILHGNKLLASEVASQIAEHERFGGVVPE
                     VASRNHLVQAPRLLERATRAAKID"
BASE COUNT          958 a         1216 c         1400 g          914 t
ORIGIN      
        1 gaaagagctg cagcaggaca acgcgcaaca aatcgagaga atgcgcgcga cggtcgacga
       61 gaagctgcaa ggaacgctgg agaaacggct cggcgaatca ttcaagctgg tgagtgaacg
      121 gttggagcaa gtgcaccgcg gactgggggc gatgcagcaa ttggcgagcg acgtaggcgg
      181 gttgcagaaa gtactcgcaa acgtgaagac gcgcggcggc tggggcgaag tgcagctcgg
      241 cgcgttactc gaacaggtgc taacgccgga ccagttcgcg cgcaatgtgc agacacggga
      301 cgagacgagc gagcgtgtcg aatacgcgat caaattaccg ggcgatgaga acggcgcgcc
      361 ggtttggctg ccgatcgacg cgaaatttcc aacggaagat tatcaccgac tactcgcggc
      421 ccaggacaag ggagatgtcg atgttgtcga gagcgcgatg aagaacctgg aagtgcagtt
      481 gaagaaaagc gcccgggaca tttgcgccaa atacatcaat ccgccgcgga cgacggattt
      541 tgcgctcatg tttctgccga cggaaggact gtatgccgaa gcaatccggc gcgtcggttt
      601 agttgagcag gtgcagcgcg actgccgggt ggtgttcgcc ggtccgacga cgctggcggc
      661 tctgctcaac agcttacaga tggggtttcg caccctcgcg atccagaagc ggtcgagcga
      721 agtttggaat ttactcgccg cggtgaagac cgagttcgga aaattcggcg aggcgctctc
      781 ggccgtaaaa gacaagctcg atcaggccgc gcgtcacgtc gacaatgttg cggtgcgctc
      841 gcgcgcgatc acaaagaagc tgcgcgacgt cgaagagctg ccttccaacc cgcagccgtt
      901 gttgaagaat ttgttaggcg actcggacga ggagtaggac cagcctccgg cttgtctgca
      961 atggttcgca agcgggacgc ttactccact ctacagcact tggtcccagt gggtcttgtc
     1021 tttgagcatg agctcgcgga ggaggcgaat gggcggcatg ccgtggttga gcaactcgtt
     1081 gtgaaatttt tgcagtgaaa acgcgtcgcc ttcctgcgcc ttgtaatcgt cccgcagttt
     1141 taagatctgc agcttgccga gtgaatagtt gaagtagccg ggatcgaacg tgccgcgcat
     1201 cgcttcggcg cgagcgggtt tttcttcgta ataacaattc tcctgaaaaa acttcgtgcc
     1261 gtcgtcgatg ctcatgtgct gggtatgcat tttaatcgcg gcgcaaagcc ggcataagcg
     1321 caacatcgcc tcggatgctt gcgccatgcg gtacttggcg gcacggctct cgtccaccgg
     1381 actagcagac ggcgacgcct tagcggcggg acttggagtc gccgttggac ttccgccgga
     1441 gtcgtgcctg ctgccggtat agccctcgtc gaccatcatc tgttcgcaat agtgcgccca
     1501 gccttcaaca aaggcgtagc ttccgaagat tttttccact ttgctcgccg gcgaagcgtt
     1561 cagccgcaag aactgcacgt agtggccggg ataaacttcg tggatcgaga tgaggtcggc
     1621 ggtgtaatag ttaaacgcgg tcagccattg ctctttctgt tcgttaggcc agtcgggctc
     1681 ggtcggcgtg acgtagaaat aggcctcggt tgcgcgtttc tcgaatggcc ccggcgtgtc
     1741 catcgaagcg aacgaggtgg cgcgccggta ttggggcgtc tctttgacgc gcggccgcac
     1801 ttccgaagga atggttacaa gatgccgttt cgtgacgaat tgacggattt ggtcgaggtc
     1861 tttgcccatt tcgggcagta gattttccgg cgtcgagtgt tcggcttgga tttgtttgaa
     1921 gacttcaagt gcaggtttgt tcggatcgat tctcctggcg gtctcggcaa aagcggtctg
     1981 ctcgtttttc agctgcgcca ttccgatttc gagaacttgt tgcggcgtaa gatcgacaag
     2041 ttccgtttcg gccagcaggc gctgaaattt ctcttccccg atggcaaaat ccggtgaggc
     2101 cttgggcagt ttctcgcgct ccaaccaggc tgcataatcc atcagcgcgg cggcagcttt
     2161 gcgattggtt tcctgaaagt cgccccgcac ctgctcgctt tttagatcgg caaccgccgc
     2221 gacgagattt ttcttcagga aatcggcggc gccgcgcgcg atttgcattg ccaactcgac
     2281 gtagggtttc ggcagcacgt cggcgagatt ggtgcgggcg gcgatcatga tgttcggcac
     2341 ctgcgactcg atcgcgatga tgctgcggac ccgatcttcg agcggcgcga agttgcgttt
     2401 gatgtagacg ttcacgtcgg ccgcgcgcgc atagaccatg gggttgcgct cgaaaattga
     2461 catgtcctgc atttgaaaca gctccttctt gatcgcggcc tgcagaatcc gcaggtcaac
     2521 ggactgccgt tggctcagct tgtccggttc aaattttttg agtcgatcgt cgaagcgccg
     2581 cagccgggcg agctcggcat cgagcgcgag ccggctgtaa tcggtgattt tgccgtcgta
     2641 ttcgtgcagc ccgagcgcgg tgccttggag tggccgcgca ttgaagtagc ccttgatgta
     2701 ctcctcggcg acggcttcgt attcggcatc gggagtgacc gtagcgaaat ggctggtgag
     2761 tggaactacc agcgcaataa gagcgaggat gcggcgcggc aggatcattg cggcgaggct
     2821 actttgagcg cggaagctag ttgagccgtt gcgcgttgcc aaaaaatttc ggatgagcga
     2881 tgcttgcgac cctaagaggc tgttctcatg agaccctgtt ttcgactcgg catgctgctt
     2941 gtatggacgt gtgtgctcgc gctgcccccg catggccggg cggcacaggt gacgaacccg
     3001 tcggaggacg ataacggcta ctcacagatt tcgattttcg ccaaagccgt gcagttgctc
     3061 cggcaggatt acgtcgacgc aaacaaaacc agctaccacg acctgatcac ggcggcgatg
     3121 aaagggatgc tcgcgtcgct ggatccacac agccagttca tggacccgga ggattttcgc
     3181 gacatgcagg aggacacgcg cagccgcttt aatggcctgg gcatcgaagt gtcgatgaag
     3241 aacggcttac caaccgttgt cacgccgatg gaagacacgc ctgcggcaaa ggccggcatt
     3301 ctctcgggtg accaaattct caagatcaac ggcgcgtcga cggaaaagat ggaacttcaa
     3361 gacgcgatca gtcttttgcg cggaccggcc ggcgggaaag tgacgctgac gttgctgcgg
     3421 ccgtcgacga aagacgtgaa ggactacacg ctcgagcgca ccgagatcaa agtgcaaagc
     3481 gtcaaaggcg cgcgtttgct cgatgcggcg ctgaccaatc cgtttaaaat cggctacgtc
     3541 cggctgattc aattcaacga accgacggcc gacgaattgt cgaaggcgct cgatcagctg
     3601 caaaagcagg gcatgcaggg gctgattctc gatctccgga acaaccccgg cgggctgttg
     3661 aacagcgcgg tcgatgtgtg cgcgcaattc ctgccgccga acacgaaagt cgtttcgacc
     3721 gaagggcgtg tcccctcgca acagcgggaa tacaccacgc cggcgaccgc caaagagcgt
     3781 ccgcattttc ccatggctgt gctggcgaac gaaggcagcg cgagtggcgc ggagatcgtc
     3841 tgcggcgcgt tcaaagattt gcatcgtgcg gtgatcattg gcgaaacgac attcggcaag
     3901 ggctcagtgc aaaatgtgat gcaactgccg gacggctcgg cgctgcgatt cacgaccgcg
     3961 aaatattaca ccccgagcaa gcaacagatt catggcaacg gcgttactcc aaacattcgt
     4021 gtgccaatga gcgcggaaca ggagcgcgcc ctcttcgcct tgcgcagcgg cgacagcgtg
     4081 aaaccagaag aagacaagga aatcatcaaa acgcgcgacc cgcagatgtt gcgcgcgatc
     4141 gacgcgctca aaggcgtaat gatttacgcc gagcaaaacg ccggcaagcc ggagtcggta
     4201 aaaaaataat tgtagaggcg cttgtgccaa gcgcctgtct aattagcttc ggcgcttgat
     4261 atgagcgccg ctacaatgcg cgatgacaat tctggcgctg gagacttcat gcgacgaaac
     4321 ggcagtcgcg attctgcacg gcaacaaact gctggccagt gaagttgctt cgcagatcgc
     4381 cgagcacgaa cgattcggcg gcgttgtgcc ggaagtcgca tcgcgcaatc atctcgtgca
     4441 agcgccgcga ttgctcgagc gcgccacgcg cgccgctaag atcgacat
//
LOCUS       DASTDG010000182         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1440720, whole genome shotgun sequence.
ACCESSION   DASTDG010000182 DASTDG010000000
VERSION     DASTDG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440720"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFO30_05880"
     CDS             <1..651
                     /locus_tag="VFO30_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9418852.1"
                     /translation="CAIIIEPIQGEGGDNHFRGEWLKKLREICDAHDILLIFDEVQCG
                     MGATGRMWCCEHFDVLPDLLAFGKKAQVCGVMAGSRIDEVKDNAFRLPSRLNSTWGGN
                     FTDMVRSTHYLRIIEEEHLVENAGKVGAYFLDQLRELQKECDFITAVRGRGMFLAFDL
                     ADAKSREQMWKGLFDLGLLTLRSGEESIRFRPALDMTAEVVDEAMDLLRQYCRGRK"
     gene            716..2263
                     /locus_tag="VFO30_05885"
     CDS             716..2263
                     /locus_tag="VFO30_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9418853.1"
                     /translation="MQTVLEKLGIAEENDGVFDGEWRGNGAKIDKISPIDGRKLASVR
                     SASAEDYEKAISRAHEAFLKWRTTPGPVRGDTVRRLGNALRELKHELGQLVTLETGKI
                     IAEGEGEVQEMIDICDLAVGQSRMLYGLTIQSERSNHRLMEQWHPLGVIAVITAFNFP
                     VAVWSWNAALAAVCGDATVWKPSEKTPLTAIAVAKICQRVCRETGADPAIFTLLIGDR
                     KSVGEKLAADARIPLVSATGSVAMGLNVAKVVHGRLGRTIMELGGNNALIVAPSADLD
                     MATRSIFFGAVGTAGQRCTSTRRVIMHESVASKLRDKLLAAYKSLPIGDPLDKNTVMG
                     PLIDKHAVDLVQDSIEKLKAEGGEVLYGGERLDGADYRGGCYMKPCLATARSDFEIVQ
                     HETFGPLLYLITYRDFNEAIAIQNGVPQGLTSAVFTNDVREAEKFMSALGSDCGIANV
                     NTGTSGAEIGGAFGGEKQTGGGRESGSDCWKQYMRRQTNTINFSSELPLAQGIQFGSD
                     DASSATA"
     gene            complement(2806..>3569)
                     /locus_tag="VFO30_05890"
     CDS             complement(2806..>3569)
                     /locus_tag="VFO30_05890"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418854.1"
                     /translation="LAREGFSGPEHIIDGKEGLFAVFKHVQYKGEPATFDGEGLVKDL
                     PDSPKSHYRILDCGMKSFPIEALSHAPLTAMMKTVKEHNIKADDVKEIKVEVIARAAD
                     ILGDPHKYRPDSKETADHSLPYCMAAGLVDGMVTPLQFKEERVRDKALIPIMDKVKVV
                     ANEEFEALFPKFQPSRVTITTNDSKLHSTRVDVPKGDPRDPMTEDEIAVKFTALGGDV
                     IGQDQCKKLQSFIMSIEAAKNLGALFELTTAREPT"
BASE COUNT          742 a          980 c         1074 g          773 t
ORIGIN      
        1 tgcgcgatca tcatcgagcc gatccagggc gaaggcgggg acaatcattt ccgcggcgaa
       61 tggctcaaga agctgcgcga gatttgtgac gcacacgaca tcctgctgat tttcgacgaa
      121 gtgcaatgcg gcatgggcgc gacggggcgg atgtggtgtt gcgagcactt tgatgttttg
      181 ccggacctgc tcgcgttcgg aaaaaaggcg caggtctgcg gcgtgatggc cggttcgcgc
      241 atcgatgaag tgaaggacaa tgcattccgg ttgccgtcgc gattgaactc aacttggggc
      301 ggcaatttca ccgacatggt gcggtcgacg cattacctgc gcatcatcga ggaagaacac
      361 ctggtcgaga acgcgggcaa ggtcggcgcg tattttcttg atcaattacg cgagctacag
      421 aaggagtgcg acttcatcac tgccgtgcgc ggtcgcggga tgttcctcgc gtttgatttg
      481 gccgacgcga agtcgcgcga gcaaatgtgg aagggcttgt tcgatctcgg tctgctcaca
      541 cttcgttcgg gcgaggaatc gatccggttt cgcccggcgc tggatatgac ggcagaggtg
      601 gtcgacgaag cgatggattt gctgcggcaa tattgccgcg ggcgcaaatg aaagtagcgc
      661 aagcttccag cttgcgtttt gttgattcgc aagccagagg cttgccccac ctttaatgca
      721 aacggttctc gagaagctcg gcatcgccga ggaaaacgac ggtgtctttg atggcgagtg
      781 gcgcggaaac ggcgctaaga tcgacaagat ttcgccaatc gacggacgca agctggcaag
      841 cgtacgcagc gcttcggcgg aagattacga gaaagcgatc tcgcgcgcac atgaagcatt
      901 tctgaagtgg cgcaccacac cgggcccggt gcgcggcgac acggttcgcc ggctgggcaa
      961 tgcgttgcgc gagttgaaac acgagctcgg ccagttggtg acgctcgaga cgggcaagat
     1021 cattgccgaa ggtgaaggcg aagtgcagga gatgatcgac atctgcgatc tcgccgtcgg
     1081 ccagtcgcga atgctctatg gcctaacaat tcagtcggaa cggtcgaatc accgcttgat
     1141 ggaacagtgg cacccgctcg gagtgatcgc agtcatcacc gccttcaatt ttccggtcgc
     1201 ggtctggtcc tggaacgccg cgctcgcggc ggtgtgcggc gacgccaccg tttggaagcc
     1261 atcggagaaa acaccgctca ctgccatcgc tgtggcgaaa atttgccagc gcgtttgtcg
     1321 cgagaccggc gctgacccgg cgatcttcac tttgctgatc ggcgaccgca aatcggtcgg
     1381 tgaaaaactt gccgcggacg cgcggattcc attggtctcg gcgaccggct ccgtcgcaat
     1441 gggtttaaac gtcgcgaaag tcgtgcatgg ccgtttgggt agaaccatca tggaactcgg
     1501 cggaaacaac gcgctgatcg tcgcgccgag cgccgacctc gacatggcta cgcgatcgat
     1561 tttcttcggt gccgttggca cagccggaca gcgttgcact tcgactcggc gcgtcatcat
     1621 gcacgaatcg gtcgcttcca aattgcgcga caaactgttg gccgcctaca agagtctgcc
     1681 gattggagat ccgctcgaca aaaacacggt gatgggcccg ttgatcgaca aacacgcggt
     1741 cgatcttgtg caggattcta ttgagaagct gaaagctgaa ggtggcgagg tgctgtatgg
     1801 cggggagcgg ctggatggcg ccgattatcg cggcggctgt tacatgaaac cgtgtctggc
     1861 tacggcaagg tccgacttcg agatcgtgca acatgaaacc ttcgggccgt tgctgtattt
     1921 gataacctac cgcgacttca atgaggcgat cgccatccag aacggtgtgc cgcaaggcct
     1981 aacgagcgca gtattcacca atgacgtgcg cgaggcggag aaattcatga gcgcgctcgg
     2041 gagtgattgt ggcattgcca acgtgaatac cggcacgagc ggcgcggaaa ttggtggagc
     2101 gttcggcggt gagaaacaaa ccggcggtgg ccgtgaaagc ggcagcgatt gctggaagca
     2161 gtatatgcgg cgccagacga acacgattaa tttttcgagc gagctgccgc tcgcccaggg
     2221 aatccagttt ggcagcgacg acgctagcag cgcgacggcg taatcccgga tagcgcaact
     2281 cgccccgctc aagctcgcat tgagtctgtt gcagacgcgc aaaccgagtg ttgtattcct
     2341 gcacgatttc gcggccggta cgcgatggcg gcaatttctc gatcatcatt ggcccggtgc
     2401 cttgttcagc gatcacctgg agtttctcag gcggtttagc tggctcgacg atttcgggac
     2461 ggcgtcgcgt gactggcgac gcccgcgacc tacgacgctc gcttgcctgc caaccccctt
     2521 cccgcgcctt atcacgtgtt actaaccaaa accgtgctca ctcgcgccgc atggctcgcg
     2581 ccgagcaaaa tacccggctt gtttcaacga ctgaagagaa aacagaaact aggatcggcc
     2641 gtggaatcgt aagccgctta ccatcgataa cagaaacccc gggacccaaa gctgcgcgca
     2701 gcgatggcgc acttggtggt tgaaacagtt ctccaggctt actcctgact gctttgtttt
     2761 ccttaaggag tggttatgaa ttcactcacg cgcgacggac ggattctatg ttggttcgcg
     2821 agccgtggtt agctcgaaca gtgcgccaag attcttcgcg gcttcgatac tcatgatgaa
     2881 gctctgcaat tttttgcatt gatcctggcc gatcacatcg ccgcccagcg cggtgaattt
     2941 gactgcaatt tcatcctccg tcattggatc gcgcggatca cctttgggga catcgacgcg
     3001 agtagagtgt aacttgctat cattggttgt gatggtcacg cggctcggct gaaattttgg
     3061 aaagagcgct tcgaactctt cgttcgcgac cactttcacc ttgtccatga ttggaatgag
     3121 cgccttgtcg cgcacgcgct cttccttgaa ctgcagcggc gtcaccatgc cgtcgactaa
     3181 tcccgcggcc atgcaataag gcagcgaatg atcggccgtt tccttggagt ccggccgata
     3241 cttgtgtgga tcgcccaaga tgtcggcggc gcgcgcgatt acttccactt tgatctcctt
     3301 aacgtcgtcg gccttgatat tgtgttcctt caccgtcttc atcatcgcag tcagcggcgc
     3361 gtgactgagc gcttcgatcg ggaaactctt cattccgcaa tcgagaatgc gatagtgaga
     3421 ctttggtgaa tcaggaagat ccttcaccaa tccttcgcca tcgaatgtcg cgggctcgcc
     3481 tttgtattgg acgtgcttga acacggcgaa caaaccttcc ttaccgtcga tgatgtgctc
     3541 gggccctgaa aatccctcac gcgccagga
//
LOCUS       DASTDG010000183         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1085313, whole genome shotgun sequence.
ACCESSION   DASTDG010000183 DASTDG010000000
VERSION     DASTDG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085313"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFO30_05895"
     CDS             <1..934
                     /locus_tag="VFO30_05895"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9418855.1"
                     /translation="TEATMSCIRLARAFTGRDKIIKFDGCYHGHADALLVKSGSGALT
                     HGRPDSAGVPQAFVDLTISLPFNDVDLVRKAFRENPNQIAAVILEPIPANAGLYFPRD
                     EFLSILREECNRHGALLIFDEVMTGFRVARGGAQQIYGIKPDLTALGKIIGGGLPVGA
                     FGGRAEIMEQLSPVGPVYQAGTLSGNPLAMAAGLAQLRELQRIDGWKLLEERGAYFEQ
                     LARDAIAQANIDLTFHRIGSMFCLFFTRGPIVDLASAQRSDLKQFAKFFHACGHRGVY
                     FAPSQFETGFISTAHITDDIDSTARVLREVLLTL"
     gene            989..3553
                     /locus_tag="VFO30_05900"
     CDS             989..3553
                     /locus_tag="VFO30_05900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9418856.1"
                     /translation="MTKIRPVAKFFFEGNRKFFVKGIAYGPFKPDADGYYVGRPEQVN
                     VDVHMMREIGLNLVRVYHAPPPWFLERCVAAGLRVLITLPWAKHIEFLRERAARKAIV
                     DTVRGAVSKYAGHPAVFGYLVGNEISSTMARWLGVRRVSDFMEQLIRIGRGIDPEVLF
                     SYATYPPTEYLLPRNVDFFCFNIYLHDQRAFEPYLLRLQNLTEDRPLVLGEFGLDTLR
                     HSENEQAQLLGWHLESVVKCGLAGTILFSWTDEWFTGEREITDWAFGIVTRERAPKQA
                     YYLLQEKLGRDSTVPHPPLSCAPFVSVVVCSYNGGRTLAACLDSLGKLNYSAYEIILV
                     DDGSTDDTAYIAAQFPHVRYVHQSNHGLSHARNTGAAAARGDVLAYTDSDCMADTDWL
                     YHLIGTLMSGQYAAVGGPNFTPPAQKWVQACVAAAPGGPSHVLLTDSIAEHIPGCNMA
                     FYRWAFDSVGGFDVQYHKAGDDVDFCWRLQQAGCVIAFSPAAIVWHHRRLTVSAFFRQ
                     QEGYGEAESLLRFKHLIFFGPTGAAKWRGQIYGTPRFSWFINRPIVYHGLFGEGFFQS
                     LYRAPQSEIASYLSSVEWFALTIFLFGLGIFLPALRIVPYLMFGGTLAVALSYMVRAR
                     IEPKFDTVHARLLVLFLAFLQPLVRGLARYFTWLHFKRTPRSVISKHEELPAGARFAG
                     PRGRQAFWSSEGRDRHYLLGSIFDLLEEEGWRYSIDTGWNDWDIQIYGNFWWSIALQT
                     VTEYHGQGECLTRVRLRNRAVPTTVAANLIALSLLIYRQLNTSHVALSVIVPYALFLV
                     FLASRARALKSRVAELVEAAAHRVGLQRMRRGRLVPVSPAAADIEVPMHVDPAARV"
     gene            complement(3550..4476)
                     /locus_tag="VFO30_05905"
     CDS             complement(3550..4476)
                     /locus_tag="VFO30_05905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9418857.1"
                     /translation="MKTGETRPIIKLLVPKYAARTRLDRFVASVLTSYSRSRLQQLIR
                     EEFITRNGAPARPRDLVRSGDEIAVSEPRPRKIDNEPEAIPLDILFEDGDLLVINKPP
                     GLVVHPGAGHREHTLVNALLHHCPALSGIGGKERPGIVHRLDKDTSGCLVVAKNDAAH
                     QSLAQQFAAREVAKIYLALVAGKLRRQAGVIEGKIGRHPVHRKRMSVASERGRAAKTE
                     YRVVGAGENASLVECRLHSGRTHQIRVHLHYIGHPVLGDKIYAPKLARSFPRQMLHAW
                     KLGFRHPRTGEWDQFEAPLPDDFRDVLASVGL"
     gene            4524..6206
                     /locus_tag="VFO30_05910"
     CDS             4524..6206
                     /locus_tag="VFO30_05910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9418858.1"
                     /translation="MSQLQTAGTTQTCPKCSAKIDTADAEPLARVPCPICGEIIRVER
                     TFDHFALVETLGIGGMATVYKARDTFLDRFVALKVVRRDLGTGVDRTLQLQQEARAAA
                     AINHPNVVQVFSSGTDRGQFYLVTELLEHGSLDDLIERRKRVSEQRLLETGIQIASGL
                     RAAQAKGLIHRDIKPANILFADEYTAKIGDFGLAGVAAQTAETRGEIWGTPYYVAPER
                     LDRQVEDFRSDIYSLGATLYHAVAGRAPIEADTTSASELRKLKQQPLDLRRIAPDVSA
                     PTAQVLQRMLAPVPAERFASYDQLIQELERALKTTTEQRKIDAPPVNRKSRPIAAIAA
                     VVLVVGFGVLVFAWNLERSITSKKARTPDNTRVQSSATPRTAAGATAQSSRGVSWEAA
                     LAGYKRHVALYDFAGARASIQAAQVSGRSAIKSQEAMASIAQWLVEWKDKLIVDLNKK
                     QFHSALTDINGVQYTGITGGNAEQLMLKTRYGIIGARWPTLSPNILLAVSASFISPRA
                     RDAADRQWLCAAFASQTGQSEEARRFAEAAAKAKSGYRRQIGLLLSPASTSR"
     gene            6279..>6399
                     /locus_tag="VFO30_05915"
     CDS             6279..>6399
                     /locus_tag="VFO30_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9418859.1"
                     /translation="MDKTLAGKKVAILVADGFEQVEMTKPREALQNAGAETKIV"
BASE COUNT         1279 a         1924 c         1837 g         1359 t
ORIGIN      
        1 caccgaagcc accatgtcat gcatccggct ggcccgcgcg tttaccgggc gcgacaagat
       61 catcaagttc gacggttgtt atcacgggca cgccgacgcc ttgcttgtca aatccggtag
      121 cggcgcgctc acgcacgggc gtcccgacag cgccggcgtg ccgcaagcat tcgttgatct
      181 gacgatttcg ctgcctttca acgatgtcga tcttgtccgg aaagcgtttc gcgaaaatcc
      241 gaatcaaatt gccgctgtaa ttctcgaacc gattccggcg aacgccggcc tttattttcc
      301 acgtgacgag ttcttgtcga tcttacgcga ggaatgcaat cggcacgggg cgcttctgat
      361 tttcgatgag gtgatgaccg ggttccgcgt cgcgcgcggc ggcgcgcagc aaatttacgg
      421 catcaaacct gatctgaccg cgctcggtaa aatcatcggc ggcggtttgc cagtgggtgc
      481 atttggcgga cgcgccgaaa tcatggagca gctttcgccc gttggtccgg tttaccaggc
      541 cggaacactg tccggaaatc ctttggccat ggcggccggg ctcgcgcagt tgcgcgaact
      601 ccagcgaatc gacggctgga aattactcga ggaacgcggc gcatactttg agcagctcgc
      661 gcgcgacgca attgcgcagg caaacattga cctcacgttt catcgcattg gttccatgtt
      721 ttgtctcttt ttcacgcgcg gaccgattgt cgatcttgcc agcgcgcagc gaagcgatct
      781 gaaacagttc gcaaaattct ttcacgcctg cggccaccgc ggcgtctatt ttgcgccatc
      841 acaatttgaa accggcttca tctcaaccgc acacataacc gacgacatcg actctaccgc
      901 cagagtactg cgcgaagttc tgctgactct gtaggggaag ctgttagctt cccacgaaac
      961 agtgagagat gtctcgactt cgcttgacat gacaaagatc cgcccagtcg cgaagttttt
     1021 ttttgagggc aaccggaaat tctttgtcaa aggcatcgcc tacggcccgt tcaaaccgga
     1081 cgcggacggc tattacgttg gccggcccga acaggtaaac gtcgatgttc acatgatgcg
     1141 cgagattggg ttgaaccttg tgcgcgtcta tcacgcgccg ccaccctggt tccttgagcg
     1201 ctgcgttgca gccggtctgc gcgttctgat tacgttgccg tgggccaaac atatcgagtt
     1261 cctgcgcgaa cgcgccgcac gcaaagcgat cgtcgacacg gttcgaggcg cagtcagcaa
     1321 atacgccggt catccagctg tgttcggtta tctcgtcggc aatgagattt ccagcacgat
     1381 ggcgcggtgg ctcggtgtgc gccgggtgag cgacttcatg gagcaactaa tccgcatcgg
     1441 tcgcggcatc gacccggagg tcctcttctc ctacgccact tacccaccga cggagtatct
     1501 cctgccgcgc aatgtggatt ttttctgttt caacatctac ctccacgacc agcgcgcatt
     1561 cgagccatat ctgttgcgcc tacaaaacct gacggaggac cggccactgg tcctgggcga
     1621 attcggtctc gatacgttgc gccattctga gaacgagcaa gcgcaactgc tcggttggca
     1681 cctcgaaagt gtagtgaagt gcggcctggc cggtaccatc ctcttctcct ggacggacga
     1741 atggttcacc ggcgagcggg agatcaccga ttgggccttt ggaatcgtca cgcgcgagcg
     1801 cgcgccgaaa caagcttact acctactaca ggagaaactc ggacgcgact cgacggtgcc
     1861 gcacccgccc ttatcatgtg cgccttttgt ctcggtggtc gtttgttctt acaacggcgg
     1921 gcggacactg gccgcatgcc ttgattcgtt aggtaaactc aattactcgg cctacgagat
     1981 catcctcgtc gacgacggat caacggatga cactgcctac atcgccgcgc aattcccaca
     2041 tgttcgttac gttcaccaga gcaatcacgg gctgagtcat gcgcgaaaca ccggcgcagc
     2101 agccgcgcgc ggcgacgtgc tcgcctacac cgactcagac tgcatggccg atacggactg
     2161 gctctaccac ctcattggaa cattgatgag cgggcaatac gccgctgttg gtggaccaaa
     2221 ctttacgccg ccagcccaaa aatgggtgca agcgtgcgtg gccgcggctc ccggcgggcc
     2281 aagccatgtg ctcctcaccg actcaatcgc cgagcacatt cctggttgca acatggcgtt
     2341 ctaccggtgg gcattcgaca gcgtcggcgg cttcgacgtc cagtatcata aagcgggaga
     2401 cgacgtggat ttctgctggc gcctgcaaca agctggttgt gtcatcgctt tctcaccggc
     2461 tgcaatcgtc tggcaccatc ggcggctgac agtgagcgct tttttccggc agcaggaagg
     2521 ttacggcgaa gccgaatcgt tgcttcgctt caagcacctg atcttcttcg gaccgaccgg
     2581 cgctgcgaag tggcgcgggc aaatctacgg aacgccgcgt ttcagttggt tcattaaccg
     2641 gccaatcgtt tatcatggcc tcttcggcga aggatttttt cagtcgcttt atcgagcgcc
     2701 acaatccgag atcgccagct acctgagcag cgtcgaatgg ttcgcgttaa ccatctttct
     2761 ttttggactc ggcatctttt tgccggccct gcgcatcgtg ccatatctga tgttcggcgg
     2821 cacgctggcg gtggcgcttt cctacatggt gcgcgcgcgc atcgagccga agtttgatac
     2881 cgtgcatgcg cggttgctcg tcctgtttct cgccttcctg caaccactcg tccgcggctt
     2941 ggcacgttat ttcacctggc tgcatttcaa acgcacgcca cgcagtgtca ttagcaagca
     3001 tgaggaattg ccggcaggcg cgcgtttcgc cggcccgcgc gggcgccagg ctttttggag
     3061 cagcgaaggg cgcgaccgcc attatctgct cgggtcgatc ttcgatttgc tcgaggagga
     3121 aggttggcgc tattcgatcg acactggatg gaacgattgg gacatccaga tttacggcaa
     3181 tttctggtgg agcatcgctc tgcaaaccgt gaccgagtac cacggccagg gcgaatgcct
     3241 gacacgcgtg cgcttgcgca atcgtgccgt gccgacgacc gtcgcagcca acttaatcgc
     3301 actcagctta ctgatctacc gacagctgaa cacgtcacat gtcgctcttt cggtaatcgt
     3361 tccttatgcc ttgttcctcg tcttcctcgc cagccgagcg cgcgcgctga aatcacgtgt
     3421 cgcggagctc gttgaagctg ccgcgcatcg tgtcggtttg cagcgcatgc gacgcggccg
     3481 cctcgttccc gtgtcgccgg cagcggcgga cattgaagtt ccgatgcacg tcgaccctgc
     3541 agctcgcgtt tagaggccaa ctgaagcgag cacgtccctg aaatcgtccg ggagcggcgc
     3601 ttcgaattgg tcccactcgc cggttcgtgg atgccgaaag ccgagcttcc acgcgtgcag
     3661 catttgacgc ggaaaacttc tggccagttt cggcgcgtaa attttgtcgc ccaaaaccgg
     3721 atggccaatg tagtgaagat gaacgcgtat ttggtgggtg cgaccgctgt gtaagcggca
     3781 ctcgaccagg ctcgcgttct cgcctgcgcc cacaacgcga tattctgtct tcgcggctcg
     3841 tccgcgctct gatgcgaccg acattcgttt ccggtgcacc gggtggcgtc cgatcttccc
     3901 ttcaatgacg ccggcttgtc tgcgcaactt tcctgcaacc agcgccaaat agatcttcgc
     3961 cacctcacgc gcggcgaatt gctgggccaa gctctggtgc gctgcgtcgt tctttgccac
     4021 gacgagacag ccgcttgtat ctttgtcgag ccgatgcaca atcccgggcc gctccttgcc
     4081 cccaatcccc gacagtgccg gacaatgatg cagcagcgcg ttgaccagcg tgtgctcgcg
     4141 atggccggct ccgggatgca cgaccagacc tggcggtttg ttaatgacga ggagatcgcc
     4201 atcctcaaac agaatatcga gcgggattgc ctccggctca ttgtcgatct tccgcggccg
     4261 cggttcgctc actgcgatct cgtcgccgga acggacgagg tcgcgtggcc gcgcgggcgc
     4321 gccgttgcgg gtgatgaatt cctcgcgaat gagctgctgt agccgagacc gcgagtagct
     4381 ggtcagcacg ctggcaacaa aacgatcgag tcgcgtccgc gcggcatact tgggcacgag
     4441 cagtttaatt atcggtcttg tctctcctgt tttcacatga gccttaaatt gcccgctaat
     4501 tgctggatgt tagactcgaa ccaatgtcgc agctacaaac cgccggcacg acgcaaacgt
     4561 gcccaaaatg cagtgccaag atcgacacag cggatgcgga gccgttggcg cgcgtgccct
     4621 gcccaatttg cggggaaata atacgcgtcg agcgaacgtt cgaccatttc gcgctcgtgg
     4681 agactctcgg catcggcggg atggccacgg tgtataaggc gcgggacacg ttcttggatc
     4741 gttttgttgc cttaaaagtc gttcggcgcg atctcggaac cggcgtcgat cgtacgctgc
     4801 aactccaaca ggaagcgcgc gccgcggcgg cgatcaatca ccccaatgtg gtgcaggttt
     4861 tttcctccgg cactgatcgt gggcagttct atctcgtcac agaactactt gagcacggga
     4921 gtctcgatga cttgatcgaa cgacgaaagc gcgtgtcgga gcaaaggctt ttggaaactg
     4981 gtattcaaat cgccagcggg ctgcgcgccg cgcaggcgaa aggcctcatc catcgtgaca
     5041 tcaaaccggc caacattctc tttgccgacg aatacaccgc taagatcggg gacttcggat
     5101 tggctggtgt ggccgcccag actgcagaaa cacgcggcga aatctggggc acaccctatt
     5161 acgtcgcgcc agaacgactc gataggcaag tggaagattt tcgcagcgac atctacagcc
     5221 tcggcgccac tctgtaccac gccgtcgcgg gccgagcgcc gattgaggca gacaccactt
     5281 ccgcaagcga attgcggaag ctgaagcagc agccgctcga tttgcgccgt atcgcgccgg
     5341 atgtgtcggc accgacagcc caggtcttgc agcgcatgct cgcacctgtg cccgcagagc
     5401 gtttcgcgtc ttacgaccag ctcattcaag aattggagcg cgcgctgaag acaaccactg
     5461 aacagcgcaa gatcgacgca ccgccagtaa accggaaatc tcggccgatc gcagccattg
     5521 ccgccgtcgt gctcgtcgtg ggattcggcg tgttggtctt cgcatggaac ttagaacgat
     5581 cgatcacaag taagaaggcc cgcacgccgg acaacacgcg cgtgcaatct tctgcaacgc
     5641 cgcgaaccgc ggccggtgct actgcgcagt catccagagg cgtttcctgg gaagcggcgc
     5701 tcgccggcta caagcgacat gttgcgctct atgatttcgc cggcgcgcgc gcgtcgattc
     5761 aagctgcgca ggtcagcggc cgttcagcga taaagtcaca ggaagcgatg gctagcatcg
     5821 cgcagtggct ggtcgagtgg aaagacaaac tgattgtcga tcttaataaa aagcagtttc
     5881 acagcgcgtt gaccgacata aacggcgtgc aatacaccgg gatcacaggc gggaatgccg
     5941 agcaacttat gctaaagaca cgctacggca taatcggagc gcgctggccg acattatcgc
     6001 caaacattct gctcgcggtt tcggcttcat tcatttcgcc gagagctcgc gatgcggccg
     6061 accggcaatg gctctgcgcc gccttcgcga gtcagactgg ccaaagcgaa gaggcccggc
     6121 gatttgccga agccgccgcc aaggcaaaat cagggtaccg tcggcagatc ggtttacttt
     6181 tgtcacccgc gtccacatcg cgatgatctg gccccgcgcg atttagacat ccgggcgcgg
     6241 cgctgtaagc gttctcacaa ctgaactggt ttgaaccaat ggataagaca ctagcaggca
     6301 aaaaggtggc gatccttgtc gctgacgggt ttgagcaggt cgagatgacc aaaccgcgcg
     6361 aagcgctcca gaatgcgggc gcagaaacga aaatcgttt
//
LOCUS       DASTDG010000184         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_943484, whole genome shotgun sequence.
ACCESSION   DASTDG010000184 DASTDG010000000
VERSION     DASTDG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943484"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="rnpB"
                     /locus_tag="VFO30_05920"
     ncRNA           complement(<1..296)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFO30_05920"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(339..602)
                     /locus_tag="VFO30_05925"
     CDS             complement(339..602)
                     /locus_tag="VFO30_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418860.1"
                     /translation="MRGFIIFVLLAVVGILFFQQKHSERPAAVPSTIRAQSAQTKSTP
                     APLAPAPRGQASEYNYMKRALDRAHDVTEQARARTKAAQDSEP"
     gene            complement(606..1292)
                     /locus_tag="VFO30_05930"
     CDS             complement(606..1292)
                     /locus_tag="VFO30_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418861.1"
                     /translation="MTSCTRVNNRLSSEQRLAAARTLLEHREDYRATFARIAFVAGTL
                     SIVLAGVTFVNDEVTRFFHRPVRPREFAFAWLVVLLLTTSLSASLCRHEARGAGQRLA
                     TEPLKLVLTNVAPLLLIPAAFTGWFFATGYLGGTELDLVVVWIAFYGLMLLSTGLFAP
                     RAITLLGWAFLLSALAVPLIQDNLDLWVGSVPTVLMGLTFGFYHLFYAGLNWRKAARP
                     MSSGSPPAAR"
     gene            complement(1289..1777)
                     /locus_tag="VFO30_05935"
     CDS             complement(1289..1777)
                     /locus_tag="VFO30_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HET9418862.1"
                     /translation="MEIFWWLFTILLFAIGLLGTVLPFLPGTTVILAGAVIHRLMLGP
                     DKSISWRVMSVLLVLTLATYALDFLSGYFGAKYFGASKWGMFGAVLGTLIGLFFGILG
                     LFAGPIIGALAGEFVAGKKLVDAGRAGWGSLLGNLAGMVSKLLIALVMITLFLVSVPS
                     PF"
     gene            1919..2413
                     /locus_tag="VFO30_05940"
     CDS             1919..2413
                     /locus_tag="VFO30_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9418863.1"
                     /translation="MPLATGTKAPDFSLPTKTAEGPKQIKLSDNFGKKNTVLLFFPMA
                     FTGVCTTEMCDVSQGLNAYRDLNAEVYGISGDNPFAQEAWAQKEKIGVTLLSDYEHKV
                     ARDYGVAYDSFLPQLNLGMADVAKRSAFIVDKTGVIQYAESSDDAKQLPNFDKIKGKL
                     AELK"
     gene            2491..>3253
                     /locus_tag="VFO30_05945"
     CDS             2491..>3253
                     /locus_tag="VFO30_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9418864.1"
                     /translation="MSDQFKTLTKFDAGSGREGWLYSLPGLEEQGIGKISRLPVSIRI
                     VLESVLRNCDGKKVRRKDVETLADWNAKKPANEEIPFVVARIVLQDFTGVPLVVDLAA
                     MRSAVKKLGGDPKIIEPLVPVDLVVDHSVQVDFFGSAQALRLNLDMEFKRNRERYQFL
                     KWGQQAFKTFQLIPPGIGIVHQVNLEYLAKGVLKSSATVPLADSKSATETVAPLYYPD
                     TLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPV"
BASE COUNT          775 a          952 c          894 g          632 t
ORIGIN      
        1 ccccgaaggt tttcggggcg ggtcggaccg gctgtcctcc ctctgttttg tcttgcaccg
       61 catggggttt ttcctgcctc gcgaattact tcgcgagcgg tgagctctta cctcgccttt
      121 tcacccttgc ctgcgctttt ctcaaagaac cggcggtgtc cttttctgtg acactttccg
      181 tcaccgcaat ttttcaactg cggcgcccgc gtgttttacg cggcacgctg ccgtctggtg
      241 ttcggacttt cctccggcaa acctttcggt tcaccagcga tcatctgcca tcggcgaata
      301 aattagcaca actccgtccg tgcgcgagtc agtcagagtt acggctccga atcctgagct
      361 gccttcgtcc gggcgcgcgc ttgttcggtg acatcatggg cccgatcgag cgcacgtttc
      421 atgtagttgt attccgaagc ctgcccgcgc ggcgccggcg ccaagggcgc aggtgtcgat
      481 tttgtttgtg cggactgcgc ccgaattgtg ctcggcactg ccgccggtct ttcgctgtgc
      541 ttctgttgga aaaatagaat tccaacgaca gcgagaagga caaagatgat gaaaccgcgc
      601 atgagttatc gagcagcagg tggcgagcca ctgctcattg gccgagccgc ctttcgccaa
      661 tttaatccag cgtaaaacag gtggtagaaa ccgaacgtca gacccatcag caccgtcggc
      721 acactgccga cccacaggtc gaggttgtcc tggatcaatg gaacagcgag cgcgctaagc
      781 agaaatgccc agccgaggag ggttattgcc cgcggcgcaa acagacctgt cgacagaagc
      841 attaatccat aaaacgcaat ccagactacc accaggtcga gctcggtgcc gccgagatat
      901 ccggtcgcaa aaaaccagcc ggtgaacgcc gcgggaatga ggagaagcgg tgcaacattg
      961 gtcagcacca atttcagcgg ctcggtggca agtctttgac cagctcctcg cgcttcgtgc
     1021 cggcacagcg atgcactcaa gctcgtcgtc agtagcagca caaccagcca ggcgaaggca
     1081 aattcgcgtg gacgaaccgg tcgatggaaa aaacgcgtca cctcatcgtt cacaaacgtc
     1141 actcctgcga gcacgatcga caatgtgccg gcgacaaacg cgatccgagc gaaggtggcg
     1201 cgataatcct ctcgatgctc gagtaaagtg cgcgccgccg ccagccgttg ctcagaagaa
     1261 agacgattgt tcacacgagt gcaggaggtc aaaacggcga aggcacactg acgagaaaga
     1321 gtgtgatcat aacaagtgca atgagcagtt tgctcaccat cccggccaga ttcccgagca
     1381 aacttcccca gcccgcgcga ccggcgtcca cgagtttctt cccggcaacg aactctccgg
     1441 cgagcgcgcc aattatcggg ccagcgaaca atcccaaaat gccgaagaaa aggccgatca
     1501 gcgttccgag cacggcgccg aacatgcccc attttgacgc gccgaaatat ttggcgccga
     1561 agtagccgct cagaaaatcg agcgcatacg tcgcgagcgt cagcaccaag agaacgctca
     1621 tgacgcgcca actgatgctt ttgtcaggac ctagcatcag tcgatggatc acagcacccg
     1681 caagaatgac ggtcgttccc ggcaggaacg gcagcacggt cccgagtaga ccaatcgcga
     1741 acaacaaaat cgtgaacagc caccagaaga tttccattca agccgagaat cgtgatccgt
     1801 gaatcgtgaa tcgtaaagag acgctcccga ttcaacgatc ctcgctctag ctttgacggc
     1861 ccagatgtcg atcttatagt tgaattccgc tgtttaactc aaaccggagg attgatccat
     1921 gccactcgca accggaacca aagcaccaga tttctcactg ccgacgaaga cggccgaagg
     1981 cccgaagcaa atcaagctca gcgataattt cggcaagaaa aacaccgtcc tgctcttttt
     2041 cccgatggcg ttcaccgggg tttgcacgac cgaaatgtgc gacgtcagcc agggcctgaa
     2101 cgcttaccgt gatctcaacg cggaggtcta cggcatcagc ggcgacaatc cgttcgcgca
     2161 ggaagcctgg gcacaaaagg aaaagatcgg cgtgactctg ctgagcgatt acgagcacaa
     2221 agtcgcccgc gattacggcg tcgcctacga ttcattcctc ccgcaactca atctcggcat
     2281 ggccgacgtg gccaagcgct ccgccttcat tgtcgacaag accggcgtca tccagtacgc
     2341 cgagagcagc gacgacgcca agcagctgcc gaatttcgac aagatcaaag ggaagctggc
     2401 cgaattgaaa tgattttcta accacgaagc acacaaagga tacgaagatt actattcctt
     2461 cgtgaccttc gtgctcttcg tggtgcaaag atgagcgacc aatttaaaac cctgacgaag
     2521 ttcgacgcgg gcagcggccg cgaaggatgg ctctattcgc tgcccgggct cgaggagcaa
     2581 ggcatcggga aaatctcacg cttgccggta agcatccgga tcgtgcttga gtcggtgctg
     2641 cgcaattgcg acggcaaaaa agtccggcgc aaagacgtcg agacgctggc agactggaac
     2701 gcgaaaaagc cggcgaacga ggaaattccg ttcgtcgttg cgcgaatcgt tctgcaggat
     2761 ttcaccggtg tgccgctggt ggtcgatctt gccgcaatgc gcagcgcggt gaaaaagctc
     2821 ggcggcgacc caaaaatcat tgagccgctg gtacctgtcg atcttgtcgt cgatcactcc
     2881 gtgcaggtcg actttttcgg ctcggcgcaa gcgctgcgat tgaatctcga catggaattc
     2941 aagcggaacc gcgagcgcta ccaatttctg aagtggggac agcaagcttt caaaacgttc
     3001 caattgattc cgcccggtat cggcatcgtt caccaagtga acttagaata tttggcgaaa
     3061 ggagtgctga aaagtagcgc aaccgtcccg cttgccgatt caaaaagcgc aaccgagacg
     3121 gttgcgccac tttactaccc ggacacgctc gtcggcaccg actcgcatac gacgatgatc
     3181 aacggcctcg gcgtcgtcgg ctggggcgtc ggtggaatcg aagccgaagc gggcatgctc
     3241 ggtcagccgg ttt
//
LOCUS       DASTDG010000185         7458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_658688, whole genome shotgun sequence.
ACCESSION   DASTDG010000185 DASTDG010000000
VERSION     DASTDG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658688"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..849
                     /locus_tag="VFO30_05950"
     CDS             145..849
                     /locus_tag="VFO30_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9418865.1"
                     /translation="MHAELEQLLVLQDRQQKIRQIQNEVKTLPLQRKSLESQLASSAA
                     GVEALKQKARQAEMDRKKLELDVGTRTETISRLKTQQYQTRKNDEFQAIGHEIQRYED
                     EIRKLEDAELEVMEQADKLKAEIEAAEKQAAVTKESVLRQLSALDEKSRALETREADL
                     TKERAELAHKIDEDLLARFERLFASKGDAAVVALEHEVCTGCHMKVTTATAMRVRAGK
                     EIVSCEQCGRILYDAA"
     gene            complement(926..2146)
                     /gene="ychF"
                     /locus_tag="VFO30_05955"
     CDS             complement(926..2146)
                     /gene="ychF"
                     /locus_tag="VFO30_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET9418866.1"
                     /translation="MDRFFDQAVFWSRALLRAEILQPPRTAVTEKVMLSAGIVGLPNV
                     GKSTLFNAVTRTHKAPAENYPFCTIDPNVGVVTIPDPRLEKLAQISHSQKIVPTAVEF
                     VDIAGLVKGASAGEGLGNQFLHHIREVDAVVQVVRCFEDSDVHHVSGSIDPIRDIEII
                     NTELALADLASLQKRRDRLQKEVRAGSKTAKIESDIIDKLLPHLDAGKPAITAQLTPE
                     EKLIARNFFLLTSKPTLFACNVAEEDLAQIAKGDQANAAMRYVDLVEDYSRHHFGTEA
                     VVISGRIESDLVDLSEKEALEYLRDLGVEDSGVHALIRAVYHLLGLRTFFTTGEKETR
                     AWTIHAGDKAPAAAGAVHSDFERGFIAAETVSYDDLVALGSIAKAREAGKLRMEGKDY
                     EVRDGDVIFFRFNV"
     regulatory      2330..2493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2567..4393
                     /locus_tag="VFO30_05960"
     CDS             2567..4393
                     /locus_tag="VFO30_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9418867.1"
                     /translation="MIPTNVKRPRNVDWKRAAAILYGDWGTSKAYVIGLAFAVGGYAS
                     FWLIAAMCVLTALVGVNYMIICRLYPDGGGVYASVRHRSEVISIVGAFLLIADYLVTA
                     AISALSGFQYLGVPHPEKFAAAAILIIGLLNLLGPKHTGGLAFLISIPTAIVVITLGL
                     FSLPHLGTAFGHLQPLHGSFWQNWSGFVGIVLALSGVEAIANATGVMRLDPGSTDAQP
                     SVRKTSTPAILWVMIEVCLFTALLGLAMHALDGLQTMNGDVNAPGAQGVRDYMLRYMG
                     EVFVGGALGPALGHLFGIIVSIVFALLLLSATNTAIVDLIAIQFLMARDREMPSLFQR
                     LNKWGVPSYGMLLATVVPMLLVVLVKDMAGLADLYAVGVVGAIATNLGATATDRKLTI
                     PKWERTLMFGTFIVMAAIEMSLLIDKPNARYFAVTILAVGLILRGLAQERRMKKEKPA
                     LAPISPAVQTSPIPSAEAGSVASIGGESILCAIRGTGRTLDFALREARETGRRLYLLF
                     VREQPFMTEEDARRKWREDPEASAIFDAAQQQAGDHPPLFAYAVSPSTAETIVDVAAT
                     LGASRLILGAPKRNALLNLLRGNVIREVSNLLPDEIDLLVYA"
     gene            complement(4390..5994)
                     /gene="serA"
                     /locus_tag="VFO30_05965"
     CDS             complement(4390..5994)
                     /gene="serA"
                     /locus_tag="VFO30_05965"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET9418868.1"
                     /translation="MAEIMQAPKILIADAIAPRGVEELARDNALEVVVKTGLTEDQLV
                     DLIPEFSAIVVRSETKVTARILSAGTRLRVIGRAGVGVDNVDVEVATRRGIVVLNAPG
                     GNTVSTAEHAFSLLLSVARKIPQADASVRSGNWKRKDFEGIELYNKTLGVIGMGRIGS
                     ELSRRAIAFGMRVLAYDPYLSATRARSLQVELVDELDDLLSTSDFISLHTPLTGETRH
                     IVDLARIRKMKRGVRIINCARGGLIDEVALAQALQDRHVAAAALDVFETEPLPNDSQL
                     RSAPNLVLTPHLGASTAEAQESVGLEIAQSVRAALLEGTIRNAVNMPSLDAKTLAIIG
                     PHLRFGEKLGRFLSQVAPRRVEQLNINYSGKVNEVDTTTITRAILKGFLKAAGGADVN
                     EVNAPAFAESLGLKINETRLSALGDYADLLELSVIAEGKTVSVGGAFFGATPRIVSIN
                     SRPVEARPHGIILVLENTDRPGMVGRIGTLLGDHGVNIATMSLSRNQAGGTALTILNL
                     DTAPSEQLLNEIRASEDVHSAQVIEL"
     gene            complement(5987..>7458)
                     /locus_tag="VFO30_05970"
     CDS             complement(5987..>7458)
                     /locus_tag="VFO30_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9418869.1"
                     /translation="LVVMGVINFFGPRHSGSLAVALAIPTVIVVILLIAISAPHFTTR
                     FLEPRHESLGALWVQFVSVILALSGAESIANLTGVMKLDPGSTMDRPSVARASLKAIT
                     PVAIEVVFGTALLGWAMLSLPSVLGQTLHLTTGGDIAAVLQQRSEDMLRFTGEQFATA
                     SFSPAVGNIFGWVVGIVFFLLLLSAANTAIVAMIGLLYMMARDREMPRQFQRLNAHGV
                     PIYPLLIAIGLPIVVLFFVANFTALAGLYAIGVVGAITVNVGSCAVNRAVGFTWYDHV
                     LFALTFVILGAVEITLAHTKPDALFFVICVLIGGLAMRAYTLKRQGLTTLTVTRELAK
                     LVTPDVIPSLQPKLAEGQKIMVAARGITPVLGFALDEAQLRKATLCVLYVKEVAIYYT
                     AAGTRLGRAKWQDDPEANAIMSSMLRLGEERDVCVVPLYAVSQDAAATIVDLAATMGI
                     DFLVIGASQRPAMAKLLRGSVATNVAQHLPESIHLVIFG"
BASE COUNT         1542 a         2177 c         2214 g         1525 t
ORIGIN      
        1 tcgcgcgtcc cgctgagttt tttgtcgacg caagcggcac agtgcgttgg gtaaatctca
       61 ccgagaacat tgccgtacgt gcgcggccgg agcaggcgct ggccgcgatt gacgccgctt
      121 tggcgcgccc ataacttcgc ccgcgtgcac gccgagctgg agcaattgct tgtcctccaa
      181 gatcgacaac aaaagattcg gcaaatccag aacgaggtaa aaacgcttcc cctgcagcgg
      241 aaaagtctcg aatcgcagct cgcgtctagt gctgccggag tcgaggcgct taagcaaaaa
      301 gcgcgacagg cggagatgga tcggaagaaa cttgagcttg atgttggcac gcgcaccgaa
      361 acgatctcgc gcctcaaaac gcaacaatat cagacgcgca agaacgatga gttccaagcg
      421 atcggccatg aaatccagcg gtacgaagac gaaatccgca agctcgagga cgccgagctc
      481 gaagtgatgg agcaggccga caaactgaag gccgagatcg aggcggcgga gaaacaagcg
      541 gcagtgacaa aagaatcggt tttgcgacag ctttcagcgc tcgatgaaaa gtcgagagca
      601 ctcgagacac gtgaggctga tctgacgaaa gaacgagcgg agctcgccca caagatcgac
      661 gaagacctac tggcgcgatt cgagcggctt ttcgcgagca aaggcgacgc cgccgttgtc
      721 gcgctcgagc acgaagtctg cacgggttgt cacatgaaag taacgacggc gacggcgatg
      781 cgcgtcaggg ccggcaaaga aatcgtcagt tgcgaacagt gtggccgcat tctctacgac
      841 gcggcgtaag ccatcgctgt agccaccggc gtgtggccgg tcatttcagc acaagcacat
      901 tgggtgacgg tccatagggc cgtggttaca cattgaaccg gaagaagatc acgtcgccgt
      961 cgcgcacttc gtagtccttg ccttccatcc gcagcttgcc cgcttcgcgc gccttcgcga
     1021 ttgaaccgag tgcgacgaga tcatcgtaag aaactgtttc cgccgcgatg aagccgcgct
     1081 cgaaatcgga atgcaccgca cccgccgcgg ctggggcctt gtcgccagcg tgaatggtcc
     1141 aggcgcgcgt ctctttttcg ccggtggtga aaaaggtgcg caggcctaac aaatggtaca
     1201 ccgcgcggat gagcgcgtgc acgccgctgt cttcgacccc gaggtcgcgc agatattcca
     1261 gcgcttcctt ttcgctcaga tcgacaagat cgctttcgat gcggccgctg atgaccacag
     1321 cttccgtccc gaaatggtgc cgcgaataat cctcgacaag atcgacatat cgcatcgcgg
     1381 catttgcctg gtcgcccttc gcaatttgcg ccaggtcctc ctccgccacg ttgcaggcga
     1441 agagggtcgg cttggaggtg agcagaaaga aattgcgtgc gatgagtttt tcttccggtg
     1501 tcaattgtgc cgtgatcgca ggcttccccg catccaagtg cggcaacaac ttgtcgataa
     1561 tgtcactctc gatcttcgcg gtctttgacc cggcacgaac ttctttttgc aaccggtcgc
     1621 gccgcttttg caaagacgcg aggtcggcca gcgcgagttc cgtgttaatg atttcgatgt
     1681 cgcgaatcgg atcgatcgag ccactgacat gatgcacgtc gctgtcttca aaacagcgta
     1741 cgacctgcac gaccgcgtct acttcacgga tgtgatggag gaactgattg cccaagcctt
     1801 cgcccgcgct cgcgccttta acaagcccgg cgatgtcgac gaattcgacc gccgtcggta
     1861 cgattttctg tgagtgtgaa atctgcgcga gtttttccag ccggggatcc ggtattgtga
     1921 cgacgccgac attgggatca atcgtgcaaa acgggtaatt ttccgccggc gccttgtgtg
     1981 tgcgtgtcac cgcgttgaag agcgtcgact taccgacgtt gggcagcccg acaattccag
     2041 cgctcagcat tactttctct gtaacggcgg ttcgtggcgg ctgcaaaatc tccgcacgca
     2101 agagcgcgcg gctccagaag accgcctggt cgaagaatcg atccacagcc gcaatcccga
     2161 ggagaaacga accgtttcga tttcgatcag ggcacggccg caggcaatgg actgtggtgc
     2221 ggatgacctc gggcttttcc cggccgtaat gacggccaga cggagtacct ttcaatgtca
     2281 ggcaaccgtc gcttgactta cttgcagcgg gccagaagtt ttcagccagt tctttgtttc
     2341 gcttaatgga ttgaccagtt gtcgatccag cgggggaacc aacctcgcgg tcgccgcggc
     2401 gacacgtgat cggggcgaac gatgcgcggc atcgagagca tttaatggct ccagcccgac
     2461 agctaacctc gtcagcatca ttaaagtgca agggatgtcg taaatccgac tccgcttgaa
     2521 gtgaggcgcc tcgtcgcgcg agggccgccc cgttgagtcg aatcagatga tcccaacaaa
     2581 cgtgaagcgt ccgcgcaatg tcgattggaa gcgcgcggcc gcgattttgt acggcgactg
     2641 gggcacgagc aaagcctacg ttatcggcct cgcctttgcc gtcggcggtt acgcatcgtt
     2701 ctggctcatc gcggcgatgt gcgtgctgac cgcgcttgtc ggcgtcaatt acatgatcat
     2761 ctgccgccta tatccggatg gcggcggcgt ttacgcgagc gtgcgccatc gcagcgaagt
     2821 catctccatc gtcggcgcgt tcttgctcat cgctgattac ttggtcacgg cggcgatcag
     2881 cgcactttcc ggattccaat atcttggtgt tccccacccg gaaaaattcg ccgcagcggc
     2941 aattctcatc atcgggttac tcaatctgct cgggccgaaa cataccggcg gtctcgcctt
     3001 tctgatttct attccgactg cgatcgtcgt catcacgctc ggacttttca gtctgccgca
     3061 tttgggcacg gcattcggac atctgcaacc gctccatggc agcttctggc aaaactggag
     3121 cgggtttgtc ggaatcgtgc ttgcgctttc cggcgtcgaa gccattgcca acgccactgg
     3181 tgtaatgcgg ctcgatcccg gcagcacgga cgcgcaaccg tctgtgcgca aaacttccac
     3241 gcccgcaatt ttgtgggtga tgatcgaggt ctgtctcttc actgcgctgc tcgggctggc
     3301 catgcacgcg ctcgacgggt tgcaaacgat gaatggcgac gtcaacgcgc caggcgcgca
     3361 aggcgtgcgc gattacatgc tccgttacat gggagaagta ttcgtcggtg gcgcgctggg
     3421 gccggcgctc gggcacctct tcggcatcat tgttagcatc gtcttcgcgt tgctgctgct
     3481 ctccgcaact aacactgcga ttgtcgatct tatcgcgatt caatttctca tggcgcgcga
     3541 ccgcgagatg ccgtcgctct ttcagcgact caacaaatgg ggcgtgccca gttacgggat
     3601 gcttctcgcc actgttgtgc caatgctgct cgtggttctc gttaaagaca tggccgggct
     3661 ggccgatctt tacgcggtcg gcgtcgtcgg cgcgatcgcc accaatctcg gcgcaaccgc
     3721 gaccgaccga aaactaacga tcccaaaatg ggagcgcacg ctcatgttcg ggactttcat
     3781 tgtgatggcg gcgatcgaaa tgtcgctgct catcgacaaa ccgaatgcgc gctattttgc
     3841 ggtgacgatt ctggctgttg gtttgattct gcgcggactt gcccaggaac gccgcatgaa
     3901 aaaagagaag ccggcgctcg cgccgatctc tccagccgtc caaacgtcgc cgattccatc
     3961 tgcggaagcg ggatcggtcg cttcgattgg aggggaatca attctttgcg cgattcgcgg
     4021 caccgggcgc acactcgatt tcgcgttgcg cgaagcgcgc gagactggcc gccgacttta
     4081 tttgttattt gtgcgcgaac aaccgttcat gactgaggag gatgccagac gcaaatggcg
     4141 ggaagaccct gaggccagtg caatctttga cgccgcccag caacaagcgg gagaccatcc
     4201 accgttgttc gcttatgccg ttagcccgtc cacggcggag acgattgtcg atgttgccgc
     4261 gacactcggc gcgtcgcgct tgattctggg cgcaccgaaa cgcaacgcgc tcctcaacct
     4321 tcttcgtggc aacgtcattc gcgaagtttc aaacttgctg ccggacgaaa tcgatctgct
     4381 ggtttacgcc taaagctcga tcacttgtgc cgagtgaacg tcttcgctgg cgcgaatctc
     4441 gttgagtaat tgttcgcttg gtgcggtgtc gaggttcagg atcgtcaacg cggtgccgcc
     4501 ggcttggttt cggctgagcg acatggtggc gatgttgacc ccgtgatcgc ctaacaacgt
     4561 cccaatgcgg ccgaccatgc ccggccggtc cgtattttcg agaaccagaa ttatgccgtg
     4621 cggacgcgcc tcgacgggac ggctgttgat gctgacgatg cgcggtgttg cgccgaaaaa
     4681 tgcgccgccg acggagacgg tttttccctc ggcaatcacg gagagctcga gcagatccgc
     4741 gtaatcgccg agcgcgctca gacgcgtctc gttgattttt aatccaagac tctccgcgaa
     4801 cgctggcgcg ttcacttcgt ttacgtcggc gccgcctgcg gccttcagga aacctttcag
     4861 aattgcgcga gtgatggtcg tggtatcgac ttcgttgact ttgccgctgt aattgatgtt
     4921 gagctgttcg acgcgacgcg gcgcgacttg cgaaagaaat cggccgagct tttcgccaaa
     4981 gcgaagatgc ggcccgatga tcgccagcgt cttggcatcg aggctcggca tgttgacggc
     5041 gttacggatc gtgccttcga gcagcgcggc gcgcaccgac tgcgcgattt caaggccaac
     5101 gctttcctgc gcttcggctg tcgaagcgcc caagtgcggc gtgagcacca aattcggagc
     5161 cgagcgcagc tgcgagtcgt taggcagcgg ctcggtctcg aagacatcga gcgccgcggc
     5221 ggcaacgtgt cgatcttgca acgcctgcgc gagcgccact tcgtcgatca ggccgccacg
     5281 cgcgcagttg atgatgcgca cgccgcgctt catttttcga atccgtgcaa gatcgacaat
     5341 gtggcgcgtc tcgccggtga gcggcgtgtg cagcgaaatg aaatcggaag ttgaaagcag
     5401 atcatcgagt tcatcgacga gctcgacctg caagctgcgc gcgcgcgttg ctgacaaata
     5461 tggatcgtac gcgagcacac gcataccgaa ggcgatggcg cggcggctga gctcgcttcc
     5521 aatacggccc atcccgatta cgccgagtgt tttgttgtaa agctctatgc cttcgaaatc
     5581 ttttcgtttc cagtttccgc tgcgcacgct cgcgtcggcc tgcggaattt tgcgcgcgac
     5641 gctgagcaaa agcgaaaacg cgtgctcagc cgtggaaacg gtgttgccgc cgggcgcatt
     5701 gagcacgacg atgccgcgcc gggtcgccac ctcaacatcg acattatcga cgccgacgcc
     5761 ggcgcgaccg atgacgcgta aacgcgtccc ggcacttaaa attctcgccg tgactttcgt
     5821 ttcgctgcgc acaacgatcg cgctgaattc cgggatcaga tcgaccagtt gatcctctgt
     5881 aagcccggtt ttcaccacga cctcgagcgc attgtcgcgg gcaagttctt cgactccacg
     5941 cggcgcgatt gcatcggcga tgagaatttt cggtgcttgc atgatctcag ccaaaaatca
     6001 caaggtgaat cgattcgggc aaatgttgcg ccacgttggt ggcgacgctg ccgcgcagca
     6061 acttcgccat cgccggacgt tgcgaggcgc cgatgacaag aaaatcgatc cccatcgtgg
     6121 cggcaagatc gacaatcgtt gccgccgcgt cctggctaac cgcatagagc ggcaccacgc
     6181 agacgtcgcg ttcctcgccg agccggagca tggaggacat aatcgcgttg gcttccggat
     6241 cgtcctgcca tttcgcgcgg ccgagtcgtg tccccgcggc ggtgtagtag atggcgactt
     6301 ctttgacata gagaacacac agcgtggctt tgcgcagctg tgcttcgtcc aacgcgaaac
     6361 cgagcaccgg cgtgatgcca cgcgctgcga ccatgatttt ttggccttcg gcgagcttgg
     6421 gctgcaggct cggaatgaca tccggtgtta ctaatttggc gagttcgcgt gtaacggtaa
     6481 gagtcgttag gccctggcgc ttcaaggtgt aggcgcgcat ggcgagacca ccaatcaaca
     6541 cgcaaataac aaagaacagt gcgtctggtt tggtgtgggc gagtgtgatc tccaccgcgc
     6601 cgaggatgac aaacgtcagg gcgaacagca cgtgatcata ccaggtgaag ccgacggcac
     6661 gattgaccgc gcacgagccg acgttgaccg tgatcgcgcc aaccacgccg atggcgtaga
     6721 gcccggccaa ggcggtgaag ttcgcgacga aaaacagcac gacaatgggc aacccgatcg
     6781 caatgagtag cgggtagatc ggaacaccgt gagcgtttaa ccgttgaaac tgccgcggca
     6841 tttcgcggtc gcgcgccatc atatagagca aaccaatcat cgcgacgatg gcggtgttgg
     6901 ccgcgctcaa gagcaagaga aagaacacga ttccaacgac ccagccgaaa atgttgccga
     6961 ccgcgggtga aaagctcgcc gtagcgaatt gttcgccggt aaaacgcagc atgtcttcgc
     7021 tgcgctgctg cagaacagcg gcgatatcgc caccagtggt cagatgaagg gtttgcccga
     7081 gaaccgaggg caacgaaagc atggcccagc cgagcaacgc tgtcccgaag acgacttcaa
     7141 tggcgacggg cgtgattgcc ttgagcgacg cgcgcgcaac gctcggccgg tccattgtcg
     7201 atcctgggtc gagcttcatt acgccggtca gattcgcgat cgattccgcg ccggagagcg
     7261 ccagaatgac gctgacgaat tgcacccaga gcgcgcccaa actctcgtga cgcggctcta
     7321 aaaaacgggt ggtgaaatgc ggcgcactga tcgcgatcag gaggattacg acaatcacgg
     7381 tcggaatcgc cagggcaact gcaagactgc cggagtgtct cggaccgaaa aaattgatta
     7441 cccccatgac caccagta
//
LOCUS       DASTDG010000186         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1042574, whole genome shotgun sequence.
ACCESSION   DASTDG010000186 DASTDG010000000
VERSION     DASTDG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042574"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFO30_05975"
     CDS             <1..459
                     /locus_tag="VFO30_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9418870.1"
                     /translation="VPANGTRVACAPLKPMTVQERVNADYATMNLTTGPHPMKLLRAQ
                     LTNIWRAIDLLHARHGATVQIAGNVICRQRPGTAKGFVFISLEDETGVANAIVDPDLF
                     ERFRLRITEESFLLIEGEVQNSDNVVLIKTRDIRPLAHEQLVGSPSHDFR"
     gene            complement(507..1991)
                     /gene="gndA"
                     /locus_tag="VFO30_05980"
     CDS             complement(507..1991)
                     /gene="gndA"
                     /locus_tag="VFO30_05980"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET9418871.1"
                     /translation="MTTNQRDIGLIGLAVMGENLVLNMESKGFSVAVFNRTTEVTEKF
                     AAARANGKNIQPTRTVEELVGALKKPRKVMMMIKAGKPVDMVIEEVAPLLDKGDVLID
                     GGNSLFTDTQRRCKDLEGKGIHYVGCGVSGGEEGALKGPSLMPGGSRESWEMIAPIFR
                     KIAAQVDGEPCCRYMGPDGAGHYVKMVHNGIEYGDMQLICEAYAILKDVVGMDAPQLA
                     EVFTDWNRGELDSYLIEITSQIFRKIDPDTGKPLVDVILDKAGQKGTGLWTLQSAIAI
                     SVVISTINAAVEARVISSRKDERVASSKVLPQPKPKKFTGDGKQLIEAIRQALYASKI
                     ISYAQGMELLGAASHQYNWNLNFGDIATIWRGGCIIRAKFLNRIVEAYQRDPALHNLM
                     LDSYFSDIIAKTQDNWRVAVSNAVEHGVAVPAFAASLAYFDSYRSARLPANLLQAQRD
                     FFGAHTYERVDRPGIFHTEWLESDHKPMQREAPPKEGPKRHATE"
     gene            2055..2504
                     /locus_tag="VFO30_05985"
     CDS             2055..2504
                     /locus_tag="VFO30_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9418872.1"
                     /translation="MANSVCDVLLTQTRLAAPAGEIDPSAGAIVDFWGVVRKLEDGRE
                     IEGIDYEAHGAMAEHQLAMIAQQAAARFELRCVIIRHRVGFVAVGEASLFLEVAAGHR
                     GAAFEASKWIVDELKRKVPIWKHPRFKVDPVKTAARSATAATVVSTK"
BASE COUNT          501 a          708 c          795 g          566 t
ORIGIN      
        1 gtccccgcaa atggcacgcg agtcgcgtgc gctcccctga aaccgatgac ggtgcaggag
       61 cgggtgaatg cggattacgc gacgatgaat ctcacgacgg ggccgcatcc gatgaaatta
      121 ctgcgcgcac aattgacgaa catttggcgg gcgattgatc tgctgcacgc gcgtcatggg
      181 gcaaccgtcc agatcgccgg taatgtgatt tgccggcagc ggcccggcac ggcgaaggga
      241 ttcgttttca tcagtttgga ggatgagacc ggcgtcgcga atgcgattgt cgatccggat
      301 ttgttcgaac gcttccggct gcgcatcacg gaagaatcat tcctcctcat cgaaggcgaa
      361 gtgcagaatt ccgacaacgt ggtgctgatc aaaacgcgcg acatccggcc gctcgcgcac
      421 gagcagctcg tggggagtcc gtcgcacgat ttcagataaa gtaacgcaag cctccggctt
      481 gcagcgcaac cgagacggtt acgctactat tcagtcgcgt ggcgtttcgg cccctctttc
      541 ggcggcgctt cgcgttgcat cggtttgtgg tcggactcga gccactcggt gtggaaaatg
      601 ccggggcgat cgacccgctc gtaggtgtgc gcgccgaaga aatcgcgttg ggcttggagc
      661 aggtttgccg gcaaccgcgc ggagcgatag ctgtcgaagt aagcaagcga cgcggcgaac
      721 gccggaacgg ccacgccgtg ctcaaccgca ttcgagacag cgacgcgcca gttgtcctgc
      781 gttttggcaa tgatgtcgct gaaatacgag tcgagcatca ggttgtgaag cgcggggtcg
      841 cgctggtagg cttcaacgat gcggttgagg aacttcgcgc gaatgatgca accgccgcgc
      901 cagatcgtgg cgatgtcgcc gaagttcagg ttccagttgt actgatgact ggctgcgcct
      961 aacaactcca tgccttgcgc gtaggaaatg attttggagg cgtagagcgc ctggcggatc
     1021 gcttcgatca attgtttgcc atcgccggtg aatttctttg gtttgggttg tggcaaaact
     1081 ttggaggaag cgacgcgctc gtctttgcgc gaagagatga cgcgtgcttc gaccgcggcg
     1141 ttgatcgtcg aaatgacaac cgagatcgcg atcgccgatt gcagcgtcca taggccggtg
     1201 cctttttgac ctgccttgtc gaggataaca tcgacaagtg gtttgccggt gtcgggatca
     1261 attttgcgga agatctgtga ggtgatttcg atcaggtaac tatcgagctc gccgcggttc
     1321 cagtcggtga aaacctctgc cagttgcggc gcatccatgc cgacgacgtc cttcaaaatt
     1381 gcgtatgcct cgcagattaa ctgcatatca ccgtactcga tgccgttgtg caccatcttt
     1441 acgtaatggc cggcgccatc cgggcccatg taccggcagc agggctcgcc atccacttgc
     1501 gcggcgatct tgcggaaaat cggcgcgatc atttcccacg attcgcgcga gccgccgggc
     1561 ataagcgatg ggcctttaag cgcgccttct tcgccgccgg acacgccgca gccgacgtaa
     1621 tgaattcctt ttccctcgag atctttgcaa cggcgttgtg tatcggtgaa aagtgaattt
     1681 cccccgtcga tgagcacgtc acctttgtcg agtagcggcg cgacctcctc aatgaccatg
     1741 tcgaccggtt tgccggcctt aatcatcatc atgactttgc gcggcttttt cagcgcgccg
     1801 acgagctcct cgaccgtgcg ggtgggctgg atgttttttc cgttcgcgcg cgcggccgcg
     1861 aacttctccg tcacttcggt cgtgcgattg aacaccgcga cggagaagcc cttggattcc
     1921 atattcagga cgaggttctc gcccatgaca gccaagccga tgaggccaat atcacgttga
     1981 ttcgtggtca ttttgagtct cctttgttga tgtgggaaaa ccggcgcacg aattgtaaag
     2041 ggaggccgaa acgcatggca aactccgttt gcgacgtttt gttgacgcag acgcggctgg
     2101 cagcgccggc tggcgagatc gatccgagcg ctggagcaat tgtcgatttt tggggcgtag
     2161 tacggaagct ggaggacggc cgcgaaatcg agggaatcga ttacgaggcg catggcgcga
     2221 tggcggagca ccagctggcg atgattgcgc aacaagcagc tgcgcgtttt gaactgcgct
     2281 gcgtcatcat ccgacatcgc gtcggctttg ttgccgtcgg tgaagcttcg ctcttcctgg
     2341 aagtcgcggc cgggcaccgc ggcgccgcgt tcgaggcgag caaatggatc gtcgatgagt
     2401 tgaagcggaa agttccaatc tggaaacacc cccggtttaa ggtcgacccg gttaaaaccg
     2461 ccgcgcggtc ggcgaccgcg gctacagttg tttcaacgaa atgaactggg cgaatcgtct
     2521 caccctgagc cgactgggtt tgacggtgct cttcgttttc gcgctcaatt
//
LOCUS       DASTDG010000187         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_440643, whole genome shotgun sequence.
ACCESSION   DASTDG010000187 DASTDG010000000
VERSION     DASTDG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440643"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /gene="ligA"
                     /locus_tag="VFO30_05990"
     CDS             complement(<1..1179)
                     /gene="ligA"
                     /locus_tag="VFO30_05990"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9418873.1"
                     /translation="MPAKEFEAAKRVAELRAQIAEHDRHYYEEAAPTISDREYDRLYK
                     ELVDLETKFPDLTAPDSPTQRVGGKPLKQFEQVAHRTPMLSLDNTYSEQEVANFYKRI
                     SKVLPDEKIPVVIEPKVDGVAVSLLYENGKLRSAATRGDGFVGDDITQNILTIRSVPE
                     RLRADGPKVIEVRGEVYMDRRGFAKLNEERKKADLPLFANPRNAAAGSLKQLDPKIVA
                     QRPLGLVCHGTGAVERADVDLHSKVFPLLKKLGLPATEKWWLADSVEGILEAIHELDR
                     IRHDFPYATDGAVVKVDAFAQRERLGFTAKSPRWAIAYKYEAERVETRLIDIIIQVGR
                     TGILTPVAVLEPVLVSGSTVSRATLHNEDEIKRKDIRIGDTVVIEKAGEVIPAVVEVV
                     Q"
     gene            1279..>2339
                     /locus_tag="VFO30_05995"
     CDS             1279..>2339
                     /locus_tag="VFO30_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET9418874.1"
                     /translation="MAGERILGVEGGGTKTAWALVERDGNELRVLKEGKLPPTNLRLT
                     SPERLHAIFDELPKQIERAGIFLAGCATADRQLVSALCSKIWPGTKIVVGSDRESGLA
                     AALGHGDGIVVNAGSGSSVTGRRGDKIENAGGWGHILGDTGGGYFLCIQALRLILREH
                     DLHRSALPVTTKILHALNLNSLDELVRWAQTADKMEVANLAPVVFAAAANGDARVMEI
                     IEEGARCLCEYVETVAQRLHLLAPKVVLIGGLFQRDSIYTHAFRRRLKKNLPDARVST
                     ADRAPELGAAWLASEMVERGSVRSRNGETQIEELAAALTEQRNPRSENLEQMSARELV
                     ELFVEEEKFVQEALRGAIV"
BASE COUNT          449 a          681 c          715 g          494 t
ORIGIN      
        1 ttgaacaact tcaactacag ccggaatgac ttcgcccgct ttctcgatca cgaccgtgtc
       61 gccgatgcgg atgtctttgc gcttgatctc gtcctcgtta tgcagagtcg cgcggctgac
      121 ggtgctgccg ctgacgagca ccggctcgag cacggcgacc ggcgtgagaa ttccggtccg
      181 cccaacctgg atgatgatgt cgatcaatcg cgtctcgacc cgttctgctt cgtatttgta
      241 ggcgatcgcc cagcgcggcg atttggccgt gaatccgagc cgctcgcgct gcgcaaaggc
      301 atcgactttg accaccgcgc cgtccgtcgc atacggaaaa tcgtggcgaa tccggtcaag
      361 ttcgtgaatt gcctcaagga tcccttcaac tgaatccgcc agccaccatt tctccgtcgc
      421 cggcagcccg agctttttta acaatggaaa aaccttggag tgaagatcga catctgctct
      481 ttccaccgcg ccagtgccgt ggcagacgag cccgagcggc cgttgcgcga caattttcgg
      541 atcgagttgc ttgagcgagc cggcggccgc attgcgcgga ttcgcgaata atggcaggtc
      601 ggctttcttc cgttcctcgt tcagtttcgc gaatccgcgt cggtccatgt agacttcgcc
      661 gcggacctcg atcaccttcg gcccatcggc gcgtaaccgt tccggcaccg accgaatggt
      721 cagaatgttt tgtgtgatgt cgtctccgac gaatccgtcc ccgcgcgtcg cggcagagcg
      781 cagcttgcca ttttcgtaga gcaacgaaac ggcaacgccg tccacctttg gttcgatcac
      841 gaccgggatt ttttcgtcag gcaaaacctt cgaaatacgc ttgtaaaaat ttgcgacctc
      901 ctgttccgaa tacgtgttat cgaggctcag catcggcgtg cgatgcgcca cttgttcgaa
      961 ttgcttgagc ggtttgccgc cgacgcgctg cgttggcgag tccggcgccg tcagatcggg
     1021 gaacttggtt tcaagatcga caagttcctt gtaaagacgg tcgtactcgc ggtcggagat
     1081 cgtcggggcg gcttcttcgt aatagtggcg gtcgtgctcg gcgatctgcg cgcgcaactc
     1141 ggctacgcgt ttcgccgctt cgaattcctt cgccggcatg gtggacgata tgaatgcggg
     1201 cggttgtaga gcaagcgcac cgcttgcttg tcaaaacaaa actcgcggcg ggcggagctc
     1261 ctgccctaca attactctgt ggcaggcgag cgtattctgg gcgttgaagg cggcggaacg
     1321 aaaacggcct gggcgctcgt cgagcgcgac ggtaacgaat tgcgcgttct caaggagggt
     1381 aagttgccgc caaccaatct gcggcttacc tcgccggaac gtttgcacgc gatttttgac
     1441 gaactgccca aacaaatcga gcgcgccggc atatttctgg ccggctgcgc gacggccgat
     1501 cgacaattgg tttcggcgct ttgctcgaaa atctggcccg gcacaaagat cgtcgtgggc
     1561 agtgaccgcg aatctggact tgccgcagcg ctgggacatg gcgacgggat tgtggtgaac
     1621 gccggcagcg gttcatctgt gactggccgt cgcggcgaca aaatcgaaaa cgccggcggc
     1681 tggggccaca ttcttggcga taccggcggc ggctatttcc tctgcattca ggcgctacgg
     1741 ctgatcttgc gggaacacga tctacaccgc agcgcattgc cggtcacgac gaaaattctc
     1801 cacgcgctga atttgaacag tcttgacgag ctcgttcgct gggcgcaaac cgcagataaa
     1861 atggaagtcg cgaacctcgc gccggttgtg tttgcagcgg ccgcgaacgg cgatgcgcgc
     1921 gtgatggaaa tcatcgaaga aggcgcgcgc tgtttgtgcg aatacgtcga gaccgtggca
     1981 cagcggctgc acttgctcgc gccgaaagtg gttttgatcg gcggcctctt tcaacgcgac
     2041 tcaatctaca cccacgcgtt ccggcgtcgg ctgaagaaaa atttgccgga tgcgcgcgtg
     2101 tcgaccgccg atcgagcacc ggaacttggc gcagcgtggc tcgcgtcgga gatggttgag
     2161 cgcggctccg ttcgcagccg caacggcgaa acgcaaatcg aggaacttgc cgccgcgctg
     2221 accgagcaac gcaatccgcg ctcggaaaat ctcgagcaga tgagcgcgcg cgagctggtc
     2281 gaactgttcg tcgaagagga gaagtttgtg caggaggcat tgcggggggc gattgtcga
//
LOCUS       DASTDG010000188         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_373632, whole genome shotgun sequence.
ACCESSION   DASTDG010000188 DASTDG010000000
VERSION     DASTDG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373632"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..952)
                     /locus_tag="VFO30_06000"
     CDS             complement(95..952)
                     /locus_tag="VFO30_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9418875.1"
                     /translation="MLRQIRNLGFDCIELGHGIRISLMPGIQEMYAAGQVKFSSLHNF
                     CPLPVEVMGASPDCYQFSSPYAHERKRAVKQTLQTIDFAQRLHAPFVVLHLGEVSMKP
                     ITDVLIGMAKNGGLLSRKYVRQKIRAVKKRERAAPVYLQRVKDCLKNIVDYAATKNVK
                     LGLEGRRGYEEIPSERELPALLDELNAPHVGYWHDFGHIQIKHNLGFLDHAEWLREIG
                     PRTIGCHVQDCIWPAQDHQPPFSGDVDLANLVPLLPRDCAWVWEMSPRKNAAEIRRSR
                     ELWKQHFGE"
     gene            complement(1009..2298)
                     /locus_tag="VFO30_06005"
     CDS             complement(1009..2298)
                     /locus_tag="VFO30_06005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9418876.1"
                     /translation="MSAELKLTTRKALSVLRPGPFRRFIIGSAISDTGTWMQVMAQGW
                     VMSTLTNKALLLGMVNFAAGLPMLALTMVGGSAADRFDKRKILLATQYAQIALAVAIG
                     LLLLSGKIAIWHIFVFAAILGVSNSFEMPTLSALVPELVKREEIQSAIGIDRAVFHGS
                     RLVGPALAGVFISFWGAASAFFANAVSFVALIIAIMTLPARRRGTIEEEEKRASGIKE
                     GFRYIAKDRPSLAMVALIAVTTIFIFPIISVMMPLYVRNVLRLGPDSLGLLMAASAIG
                     SVVGALFLLSIPRDKRVPLMMTFVALVGCAIFGLARATTFRGAVGLLIVNSLGLSMNF
                     GLANTIVQERAPDYLRGRVSAVFGLSFFGLMPLAGLVITSISDLIGMRNALMLGAIIY
                     ATIGLLVLARVRRQCAEPGVSEIEGAETPPPPVVATV"
     gene            complement(2373..3002)
                     /locus_tag="VFO30_06010"
     CDS             complement(2373..3002)
                     /locus_tag="VFO30_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418877.1"
                     /translation="MERETESGKKEAELPSSIPEIYFDIIARVVPGAIAIAAYNFDSL
                     SKNLNTPTISFALLCSYFLGMLLEIVGDSIWNNTFYKLQPGLRAKTLFWGRLKLFDFD
                     SLKDIWEWLRKLPAAERPVFTKMTAERSMFQTTALLFLFWIFFPPQLVVEKVSHQFHI
                     PARCITLLGFLACFHGMITLHTWLSWNKQVFSRNSHSAPRHGESHHAAR"
     gene            3069..>3838
                     /locus_tag="VFO30_06015"
     CDS             3069..>3838
                     /locus_tag="VFO30_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9418878.1"
                     /translation="MPSESRSLFWSVRIDKFTQKMQEALQTAQDLASQFNHQEITNEH
                     FLSALLDQPEGITQPLFEKIGVSPTGVRERLASELERRARVHGAAVDLRLSNELRTVL
                     DASEKEMAKLKDEYTSAEHYLLALSAANVPAAKMLKDLGVTRDKLMQALQQVRGSQRV
                     TDQNPEGKYQALEKYGRDLTALARRGKIDPVIGRDNEIRRIMQVLSRRTKNNPVLIGD
                     PGVGKTAIVEGLARRIISGDVPDSLKNKRVVAMDIGAMV"
BASE COUNT          894 a         1076 c         1127 g          741 t
ORIGIN      
        1 gcagccattt gcgcgcgctg gtgtggatca tgaaacaccc aaaaaagcag gctgatggtg
       61 acggcgagct ggacaatagt gatcagaatt tttttcattc gccgaaatgt tgtttccaca
      121 gttcgcgtga gcggcggatt tcagcggcat tcttgcgcgg actcatttcc cagacccacg
      181 cgcagtcgcg tggcaacaac ggaacaaggt ttgcaagatc gacatctccg ctgaacggcg
      241 gctggtgatc ctgcgccggc cagatgcaat cctgcacgtg gcagccgatc gtgcgcgggc
      301 cgatttcgcg cagccattcg gcgtgatcga gaaagccaag attatgttta atctggatgt
      361 gaccgaagtc gtgccagtag ccgacgtgcg gcgcatttag ctcgtccagc aacgccggca
      421 attcgcgctc gctcggtatt tcttcatagc cgcggcggcc ttcgagaccg agcttcacgt
      481 ttttcgtagc ggcgtaatcg acaatgtttt ttaagcagtc tttgacacgc tgcaaatata
      541 ccggtgcagc tcgctcgcgt ttttttaccg cgcgaatttt ctggcgaaca tatttgcgcg
      601 agagcagacc gccatttttc gccatgccga tgagcacgtc cgtgattggt ttcattgaaa
      661 cttcacccag gtgcaggaca acaaacggcg cgtggaggcg ttgggcgaaa tcgattgttt
      721 gcaaggtttg cttgaccgct cgtttgcgct cgtgcgcgta gggcgacgag aactggtagc
      781 aatcaggcga ggcacccatt acttcgaccg gcagcggaca aaaattgtgc aggctgctga
      841 attttacctg gccggcggcg tacatctcct gaattccagg catgagcgaa atgcggatgc
      901 cgtggccgag ttcgatacaa tcgaagccaa ggttgcggat ttgccggagc atcttgtcgc
      961 cggcggtatt gcgtgccgaa ttccaacacg tggaaaatgc gatcacacct agacggtcgc
     1021 gacgactggc ggaggcggtg tttcggcgcc ctcgatttcg ctcactcccg gttcggcgca
     1081 ctggcggcgc acgcgcgcga gtacgagcaa gccgatcgtc gcgtagatga ttgcgccgag
     1141 catgagcgcg ttgcgcatgc caatgaggtc ggagatgctc gtgatcacca gccccgcgag
     1201 cggcatcaaa ccgaagaagc tcaaaccgaa aacggccgag acgcggccgc ggagataatc
     1261 cggcgcgcgc tcctgcacga tggtgttcgc cagaccgaag ttcattgaca gaccgagcga
     1321 attcacaatc agcaagccga ccgcaccgcg aaatgttgtc gcgcgcgcca aaccaaagat
     1381 cgcgcagccg accagcgcca cgaatgtcat catcagtgga acgcgtttgt cgcgtggaat
     1441 actgagcaga aaaagcgcac cgacgacaga cccgatggcg gacgccgcca tcaaaaggcc
     1501 taacgagtcc gggccgagcc ggagcacgtt gcggacgtaa agcggcatca tcactgagat
     1561 gatgggaaag atgaagatcg tggttaccgc gatcagcgcc accatcgcca ggctcggcct
     1621 gtctttcgcg atgtaacgaa agccttcctt gatcccactg gcccgtttct cctcttcctc
     1681 gatcgtgcca cggcggcgcg ccggcagcgt catgatcgcg atgatgagcg cgacgaagga
     1741 aacggcattg gcgaagaacg ccgaggcggc gccccaaaaa ctaatgaata ctccggccaa
     1801 agcgggaccc accaggcgcg acccgtgaaa caccgcgcgg tcgattccga tcgcgctttg
     1861 aatttcttcg cgcttcacca actccggcac gagcgcggag agcgtcggca tctcaaacga
     1921 attcgaaacg ccaaggatgg ccgcgaaaac aaagatgtgc cagatcgcga tcttgccgct
     1981 gagcaagagc agaccgatcg cgacggcgag cgcgatctgc gcgtactgcg tcgcgagtaa
     2041 gattttgcgt ttgtcgaaac gatcagcggc cgacccgccc accatcgtga gcgcgagcat
     2101 cggcagaccg gcagcgaagt tcaccatccc gagcaggagg gctttgttgg tgagcgtgct
     2161 catgacccag ccttgcgcca tgacctgcat ccacgtgcca gtgtcggaga ttgcgctgcc
     2221 gatgatgaag cggcgaaacg ggccgggccg caaaacggac agcgctttgc gagtggtcag
     2281 tttcaactcc gcgctcatgg gctgcacaca taaccggaga aaagcgcgac tgcaacgccc
     2341 gtggtcagcg ttcaccaggt tgaatcagaa actcaccgag cggcgtggtg actttcgccg
     2401 tgtcgcggag cagagtgaga gttccgactg aagacctgtt tgttccaact gagccaggta
     2461 tggagcgtga tcattccgtg aaagcaagcg agaaagccaa gcagagttat gcatcgcgcc
     2521 ggaatgtgaa attggtgcga caccttctcg acgacaagct gcggcgggaa aaatatccag
     2581 aacaggaaaa gaagcgccgt ggtttggaac atcgacctct ccgccgtcat ttttgtaaag
     2641 acagggcgtt cagcggcggg caattttcga agccattccc aaatgtcttt gagcgagtcg
     2701 aagtcaaaga gcttcagtct cccccaaaaa agcgtcttcg cgcgcaaccc aggctgcaac
     2761 ttgtaaaacg tgttgttcca aatggagtcg ccgacgatct ccaaaagcat tcccagaaaa
     2821 taagagcaca gcagagcaaa actaattgtt ggcgtattga gattcttgga cagggaatca
     2881 aagttgtagg ccgcaatcgc gatcgcgccc gggacaactc gcgcgatgat gtcgaaatag
     2941 atttccggaa tcgaagacgg cagttctgct tctttcttgc ctgattcagt ttctctttcc
     3001 atgggggctg cgcctattgc agcaataaaa cttcgggaac tgaagactta tcctcacaag
     3061 ggacgcaatt gccgagcgag tcacgttcgt tattttggag cgtgcggatc gacaagttca
     3121 cgcaaaagat gcaggaagcg ctgcaaaccg cgcaggacct cgcttcgcag ttcaatcatc
     3181 aggaaatcac gaatgaacat ttcctgtctg cgctgctcga ccagcctgaa ggcataactc
     3241 agccgctctt cgagaaaatc ggcgtctcgc cgacgggcgt gcgggaacgc ctcgcgagtg
     3301 agctcgagcg tcgagcacgc gtgcacggcg ccgctgtcga tcttcggctt tcaaacgagc
     3361 tgcgcaccgt gcttgacgca tccgaaaaag aaatggcgaa gctgaaggat gaatacacca
     3421 gcgccgagca ttacctgctc gcgcttagtg ctgccaatgt tccggccgca aaaatgttga
     3481 aggatctcgg cgtgacccgc gacaaattga tgcaggcgct gcagcaggtg cgcggctcgc
     3541 agcgcgtcac cgatcagaac ccggaaggta aataccaggc actcgagaaa tacggtcgcg
     3601 atctgacggc gctcgcgcgg cgcggcaaga tcgatccggt gatcgggcgc gataacgaga
     3661 tccgccggat catgcaggtg ctttcgcgcc ggaccaaaaa caatccggtg ctcattggcg
     3721 atcccggtgt cggtaaaact gccattgtcg aagggctcgc ccgccggatc atcagcggcg
     3781 atgtgcccga ttcgttgaag aacaagcgcg tagtcgcaat ggacatcggc gcgatggt
//
LOCUS       DASTDG010000189         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_754213, whole genome shotgun sequence.
ACCESSION   DASTDG010000189 DASTDG010000000
VERSION     DASTDG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754213"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFO30_06020"
     CDS             complement(<1..230)
                     /locus_tag="VFO30_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418879.1"
                     /translation="MKTPAMNPTAALARSRLLADFAELVKARLTLLVLLTTAVGYYLG
                     APSPIDYTGLFHVVFGTAAAAAGAAALNEWWER"
     gene            complement(227..1318)
                     /locus_tag="VFO30_06025"
     CDS             complement(227..1318)
                     /locus_tag="VFO30_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9418880.1"
                     /translation="MTVAFIFRPGRFQVVDDAISKSAMLAERRYSRWLNRFAWFTAVA
                     TLLLICSGGMVTSKGVGLAVPDWPTTFGYNMFMFPVSKWIGGIFFEHTHRLIASTVGV
                     LTLILAIWIWGAEDRQSLRNLALIAVAGVIFQGILGGLRVAMLKDQIGIFHACVAQAF
                     LGLIVLIALITTKLWRSVSNTVVDRASTKRLQTLAMAITVAIYGQLALGAIMRHQHRD
                     LAILDFPTANGAWIPDTSPAALAKINSWRDMHGFSDVSAFQIWLQLAHRFLAVLIAIG
                     VIAFCLRVWRDTQDCLVLQRLSMWWVIGIFVQLTLGAWTIWSNKAADIATAHVAVGAV
                     MLCFGVSLWAICWRLCQPSRRTEPQRVLV"
     gene            complement(1344..2240)
                     /locus_tag="VFO30_06030"
     CDS             complement(1344..2240)
                     /locus_tag="VFO30_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9418881.1"
                     /translation="MSSLALINELLGQPANASEHGYQIDHILEFCHWFMGALFVGWSA
                     FFIFVLIRFRKGRHPVADHHGVRSGISTHLEFSVVLIEAVLLLGFAIPLWAKRVNEFP
                     STKDAILVNVVGQQFNWNFHLPGPDGQFGHRDIGLVTNSNPLGLDPNDPASKDDLVML
                     GELHVPVDKPVIIELSSKDVIHNFDLPHMRIAADAIPGSLIPMWFKPLKTGTYEVVCG
                     QLCGLGHYGMKGTLVVDTPADYQAWLKERAELAGQQNAPTPTQRPPGEPPVGPTPGTV
                     APPGAPKTEKPSAPVPSPSPSA"
     gene            complement(2233..>2442)
                     /locus_tag="VFO30_06035"
     CDS             complement(2233..>2442)
                     /locus_tag="VFO30_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418882.1"
                     /translation="QQAAACPFCYGAKDGKSTQHMAVAIWFLFGAVLSVIGGIGAFSF
                     KIWRHGRMPLEPHQQLAEEDLEKYE"
BASE COUNT          566 a          712 c          696 g          468 t
ORIGIN      
        1 cgttcccacc actcgttcaa cgctgctgca cctgccgcgg cggccgcggt accaaataca
       61 acatgaaaga gtccagtata atcaattgga cttggcgcgc ctagatagta gccgacagca
      121 gtggttaaaa gcacaaggag cgtcagccgc gctttaacta gctcggcgaa atctgcgagg
      181 agtcggctgc gcgccagagc cgcggtagga ttcattgccg gcgttttcat acgagcacgc
      241 gctgcggctc agtccgtcgg cttggctggc atagccgcca gcaaattgcc cagagactca
      301 caccgaaaca taacatgacc gcgccgaccg ccacatgagc cgtcgctata tccgcggctt
      361 tgttactcca aatggtccac gcgcccagcg tgagttgcac gaaaatcccg attacccacc
      421 acatcgacaa tcgctggagc acgagacaat cctgtgtgtc tcgccacacc cgcaaacaaa
      481 aagcgatcac gccgattgcg atgagcacgg ccagaaaccg atgcgccagt tgcagccaaa
      541 tctgaaacgc gctcacatcg gaaaacccat gcatgtcgcg ccaggaattg atctttgcca
      601 gagcggcagg gctcgtgtcg ggaatccacg cgccgttcgc ggtcggaaaa tcgaggattg
      661 ccaggtcgcg atgctggtgt cgcatgattg cgccgagcgc gagctgcccg taaatggcaa
      721 ccgtaattgc cattgccaga gtttgaaggc gtttggtgct tgcgcgatcg acaacggtat
      781 ttgaaaccga gcgccaaagc ttggttgtta tcagcgcgat caggacaatt aatccgagaa
      841 acgcttgcgc gacgcaggcg tgaaaaatcc cgatctggtc tttcagcatc gccacgcgca
      901 ggccgccgag gatgccctgg aaaatcacgc ccgcgactgc gatgagggcg aggttacgaa
      961 gtgattgtcg atcttccgca ccccaaatcc agatcgcgag aatgagggtg agaactccga
     1021 ctgttgaggc aatcaaccga tgtgtgtgtt cgaaaaaaat tccgccgatc cattttgaga
     1081 ccggaaacat gaacatgttg tagccaaacg ttgtcggcca atcgggaaca gccagtccga
     1141 cgcctttgct cgtgaccatt ccgccgctgc aaataagcag cagcgtcgcg acggctgtga
     1201 accacgcgaa acgattgagc catctgctgt agcggcgctc tgcgagcatc gcactttttg
     1261 aaatcgcatc gtccacgacc tgaaacctac ccggccggaa aataaatgcg acggtcatag
     1321 accgccgcta cagaaacggc cccttacgcg ctggggcttg gactgggcac gggtgcgctt
     1381 ggcttttcgg tcttcggcgc gccgggcggc gccacggttc ctggagtcgg gccgaccggc
     1441 ggttcgccgg gcggacgttg cgtcggcgtc ggcgcatttt gttgtcccgc aagctcggcg
     1501 cgctctttca accacgcctg ataatcagcc ggtgtatcca cgacgagcgt ccctttcatg
     1561 ccgtagtgac cgaggccgca aagttggcca cagaccactt cgtaagttcc ggttttcagc
     1621 ggcttgaacc acatcggaat caaggaaccg ggaatcgcgt cggccgcgat gcgcatgtgc
     1681 ggcaaatcga aattgtggat cacatctttg gaggacagct cgatgatcac cggcttatcg
     1741 acagggacgt gcaattcccc gagcatcacc agatcgtctt tgctggccgg atcgttcggg
     1801 tcgagcccga gcggattcga atttgtgacg agaccaatgt cgcggtgccc gaattgtccg
     1861 tccggtccgg gcaaatggaa gttccagttg aactgctgac cgacgacatt gacaagaatc
     1921 gcgtctttgg tggatgggaa ctcattcacc cgttttgccc aaagcggaat ggcgaaaccg
     1981 agcaaaagca ccgcctcgat tagaacgacg gaaaactcaa ggtgtgtcga aattccgctg
     2041 cgcacgccgt gatgatcggc caccggatga cggcctttcc ggaaccggat gaggacaaaa
     2101 atgaagaaag ccgaccagcc gacaaagagc gcgcccatga accaatggca gaactcgagg
     2161 atatgatcga tctggtaacc gtgttcggaa gcgttcgccg gctgcccgag caactcattg
     2221 atgagcgcga ggctactcat atttctccaa atcttcctcg gctaactgct ggtgcggctc
     2281 gagcggcatg cggccgtgcc gccaaatctt gaaactaaac gcgccgattc cgccgatcac
     2341 cgagagcacg gcaccgaaga gaaaccagat cgcaacggcc atgtgctgcg tcgatttgcc
     2401 gtctttcgca ccgtaacaga acgggcaggc cgcggcttgt tg
//
LOCUS       DASTDG010000190         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1187102, whole genome shotgun sequence.
ACCESSION   DASTDG010000190 DASTDG010000000
VERSION     DASTDG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187102"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1822
                     /locus_tag="VFO30_06040"
     CDS             101..1822
                     /locus_tag="VFO30_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418883.1"
                     /translation="MPILQRLEEPIERTQRTFIKLLLGTLAAIVVVVVLAWGGWRGYQ
                     RWEERHQVRQANAFIEHGDFKSAGLSARRALQLNLNSTGAMRIMAQLAEKAHDRVALD
                     WRRKVLQLEPGSIDDAIALSDCALQFGDIRTADKTLVGINAAGKNTADFHAAAGRLAK
                     ARKNPTEAKREFAQARQLAPSNESYQLEYALACLDQSDAAARAEGVAILGQLRNSPFQ
                     RAAATRALLLDGAAHRHDPEELRNFARDLQNYPEASFADRLLYLDVLRQLRDREYAAY
                     LSQVEKDASAKPADLAALLSWMNINSISLVAIDFAKSLPVDVLTTWPVPLALAEAHSK
                     LNDWPQLQKLAQTGNWGEFEFLRHAYLARAFRGENNPAAATREWTDGTRAASARPQSV
                     LMLARTIYDWGWKNESIDLLWQLTKYAETQFEALRALYVYYQKGHDTQGLYRVLSRLS
                     EIDPEDVKVQNNLAQVSLLLNVDLERAGKRAAELYRTESSEPAYVSTYAFSLYAKGEA
                     QAAVNAMSKLREDQLQEPSMAAYYGIFLAATGDKEKARAYLARGRQADLLPEEKALLD
                     RMELTAK"
     gene            complement(2543..2707)
                     /locus_tag="VFO30_06045"
     CDS             complement(2543..2707)
                     /locus_tag="VFO30_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418884.1"
                     /translation="MTVALGTGLMPWLSGALGTGELPGTKVAEGRTLGLALGLKPTPG
                     LSAGDPPGVN"
     gene            2876..3886
                     /locus_tag="VFO30_06050"
     CDS             2876..3886
                     /locus_tag="VFO30_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9418885.1"
                     /translation="MFRFLTRPPPSVAFAGSLVVLFGIGWLDLATGHQVSLVLFYSAP
                     IVFAVWLCDNKSAFAVAGLAGVLWSWADILLGYSYSSAAVRAWEISIRFAFFFLVALA
                     GIATKERHRASVARIKLLEHSRKLEQQIIEVSEYEQQRIGRDLHDGLCQYLAAVGCAA
                     SSLKVDLEELGFEKLAASAAEIEKLLGESVRQARNLAHGLMPVQLDDAGLPAALQELA
                     TSTSRLLRMDCTFEFAGENRFTHPGKAIHLYRIAQEAINNATKHGRARNVEVRLSANR
                     SAMSLSIADDGTGFSKSEKDRHGVGISIMRYRANVVGGEFEIEERPTCGTIVSCTVPA
                     ET"
     gene            3890..>4455
                     /locus_tag="VFO30_06055"
     CDS             3890..>4455
                     /locus_tag="VFO30_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9418886.1"
                     /translation="METLNAARQNSQKFSVVLVEDHPMFREQFRHLINKEPDMRVCGE
                     SDDCRGALELLQSVAADIVIVDLTLKNSNGLELIKDLKARKIDIPVLVVSMHDELLYA
                     ERVLRAGARGYITKHETSKEVMTAIRKVLSGQIYLGPEMASRVIESLSARPKIENGVS
                     LLTDRELEIFELIGRGRTTRQISGYLHLG"
BASE COUNT          995 a         1262 c         1323 g          875 t
ORIGIN      
        1 ggtagcggaa ttcaagccag ttgcgagccg ccctcagccg gacgacagac agaaaaagag
       61 ttgcagctgc gatggcgcgc gattggcttg tcgcaatcat atgcctattc tacaacggtt
      121 ggaagaaccg atcgaacgga cccagcgcac gtttatcaag ctgctgctgg ggactttggc
      181 ggcgattgtc gtcgtcgttg tgctggcgtg gggcgggtgg cgcggttacc aacgctggga
      241 agaacggcat caggtgcgtc aggccaatgc gttcatcgaa cacggcgact ttaaatcggc
      301 cggcttaagc gcgcggcggg cgctgcaact caaccttaac agcaccggtg cgatgcgtat
      361 catggcgcaa ctggcggaga aggcgcacga ccgcgtggcg ttggattggc ggcgcaaagt
      421 cttgcaactc gaacccggtt cgattgacga tgcgattgca ttgtccgatt gcgcattgca
      481 atttggcgac atccgcacag cggacaaaac ccttgtcgga atcaacgctg ctggaaagaa
      541 cacggcagat tttcacgccg cagccggtcg cttggctaag gcgcgcaaaa acccgaccga
      601 agcgaagcgc gagtttgcgc aggcgcgaca gctggcgcca agcaacgagt cttaccagct
      661 ggaatacgcg ctggcttgtc tcgaccagag cgatgcggcg gcgcgcgctg aaggcgtagc
      721 cattctcgga caattacgca actcgccttt ccagcgggcg gccgccaccc gcgccctcct
      781 gctcgatggt gcggcccatc gtcatgaccc tgaagaattg cgaaatttcg cgagagattt
      841 gcagaattac cctgaagcct cgtttgccga tcggctactt tatctcgacg tgctgcgaca
      901 actgcgtgac cgcgagtacg cagcttactt gagccaggtc gaaaaagacg cgagcgcgaa
      961 accagccgac ctcgcggcgt tgctctcatg gatgaacatc aacagcataa gcttggtggc
     1021 gatcgatttt gccaaatcac tcccggtgga tgtcttgacc acctggccag tgccgttggc
     1081 attggccgaa gcgcacagta agctgaacga ttggccgcag ctgcaaaagc tggcgcaaac
     1141 cggcaattgg ggagagttcg agtttcttcg acacgcctac ctggcgcgtg ccttccgcgg
     1201 tgagaacaac ccagctgcgg cgacgcgtga gtggaccgat gggacgaggg cggcttcggc
     1261 ccggccccaa tcagtgctca tgcttgcgcg cacgatctac gattggggtt ggaagaacga
     1321 aagcatcgat ctgctttggc agctcactaa atacgccgag acgcaatttg aagcgttgcg
     1381 tgcgctttac gtttactacc agaagggcca tgacacgcag ggtctctacc gcgtgctctc
     1441 acgtcttagt gagatcgatc cagaggacgt gaaggtccag aacaatctcg cgcaggtctc
     1501 cttactcctg aatgtcgatc ttgaaagggc ggggaaacgc gcagccgagc tctaccgcac
     1561 agaatcgtcc gagccggcct atgtctccac ctatgcgttt tcgctttacg ccaaggggga
     1621 agctcaggct gcggttaacg cgatgagcaa actgcgggag gatcagttgc aggagccctc
     1681 aatggctgcc tactacggca tctttctcgc cgccaccggc gacaaagaaa aagcgcgcgc
     1741 ttatctcgcc cgcggcagac aagccgacct gttgccggaa gagaaagcac ttctcgatcg
     1801 aatggagctt accgcgaaat aacccaacta cccaggtccg gtgccgaaag taccgcgcga
     1861 ttataatttg accgagagtt ccggccttgc cataaaccac gacgaggagc caaatgaacc
     1921 gcagtaacat acacagcctg cggcgggcaa aggcgatcgc tactgcggta tgcgtctgct
     1981 gcactacgtc cttgatcgcc gcgccggggg accaagcgag tcatgcgact gccggtctgt
     2041 cgaatgcaca atcaggctcc ggctccagtt ccgagaataa cccaggattg atcggcagag
     2101 aggcgagcga cagcggagac aatcctggct tgaactcgga taacaaccgg ggtaatgcgg
     2161 atggtgaatt cggccgaacc cccgcgaccc aggccgcttc gaatgggtca gaacaccgca
     2221 aggtcggcgg cggttttttc catgtcggga gagaggaagt aatggaagct cgcggcgaga
     2281 aaagcgagag cggcaaagct gaaaagacga gcgagaaaag tgaagtcacc acaagattcg
     2341 aatcgagttt gctttatgcc ggcatcacac ccgcgccgag cgagacgccg accgcgaacc
     2401 agttcggcgc cacgaacaat cccggactcg aacacgtgag tcgacaagga ttgacaagct
     2461 ccgagagcgg tcgcagtacg gcggaagctg cgcgaagtga tacgaaggcg tcgccggaat
     2521 cgagcgtgtc gccggcaact actcagttaa caccgggcgg atcgccagcg gagagtccgg
     2581 gcgtgggctt aagcccgagc gctagtccga gtgtccggcc ctccgcaact ttcgtccccg
     2641 gcagctcccc ggttccgagc gcgcctgaga gccacggcat caacccggtg ccgagcgcca
     2701 cggtcacggc caatcgctaa gatcgacaat tacgcgctga gcgcacgccc gactgcgacc
     2761 cgaatggatt ccgcggccag taaaggtcgg cgtgccaatt ttgggccgca agaccgaggc
     2821 cgttttggcg ggaaatttgc ttcccaatac ggagccttca ggatggggac ttggtatgtt
     2881 tcgcttctta acccgcccgc caccgtcagt cgcgttcgcc ggatcgttag tagtgttatt
     2941 tggtatcggt tggctggact tggccactgg tcatcaggta tcgctggtcc ttttctacag
     3001 cgcgccaatt gtgttcgcag tttggctgtg tgacaacaaa agcgcctttg ccgtcgccgg
     3061 attggcaggt gtgctttggt cttgggccga cattctattg ggttattcct attccagtgc
     3121 ggctgtgcgg gcctgggaga tttcgattcg ctttgcattt ttctttctcg ttgctctagc
     3181 cggaattgcc accaaagaac gccatcgcgc ctcggtcgcg cgcatcaagt tgctcgagca
     3241 ttcacgaaaa cttgagcagc aaattatcga ggttagcgag tacgagcagc agcggatcgg
     3301 ccgcgatttg catgatggtc tttgccaata tctcgccgcg gtcggttgcg ctgctagttc
     3361 actaaaggtc gacttggaag agctcggctt tgagaagttg gccgccagtg ccgccgagat
     3421 agaaaaactg ctgggcgaat cggtcagaca agcgcgcaat ctcgctcacg gactcatgcc
     3481 ggttcaattg gacgacgcgg gcctgccggc ggcgcttcag gaactggcca cttcgacaag
     3541 tcggctgctt cggatggatt gcacatttga attcgccgga gaaaaccgat tcacccatcc
     3601 tggcaaagcc atccatcttt accgcattgc tcaggaggcc atcaacaacg cgaccaaaca
     3661 tggcagagcc agaaacgtgg aggtccgatt gagcgccaac cgcagtgcga tgagcttgag
     3721 catcgccgac gacggaaccg gtttttcgaa aagcgaaaaa gatcggcatg gcgtcggaat
     3781 cagcatcatg cggtatcgcg ccaacgtggt cggcggcgaa ttcgagatcg aggagcgccc
     3841 aacctgcggc acgatcgttt cgtgcaccgt tcccgccgaa acgtgagtta tggaaacgct
     3901 taacgctgcc cgacaaaact cccaaaagtt cagtgtcgtt ctggttgagg atcatccgat
     3961 gtttcgtgag cagtttaggc acctgattaa caaagagcca gacatgcgcg tgtgcggcga
     4021 gagtgacgac tgccgcggcg cgttagaatt actgcagtcc gtcgcggccg acatcgtgat
     4081 tgtcgatctt accctcaaaa attccaacgg gctggagctg attaaagatc tgaaggcgcg
     4141 caaaatcgat attccagtgc ttgtcgtttc gatgcatgac gagctgctct atgccgaacg
     4201 tgttctgcgg gccggcgcgc gcggctacat aaccaaacac gaaacgtcaa aggaagtgat
     4261 gaccgccatc cgcaaggtct taagcggcca gatctacctt gggccggaaa tggcgtcccg
     4321 tgttatcgag tccctctcgg ctcggccaaa aatcgagaat ggcgtctcgt tgctgaccga
     4381 ccgtgaactg gagatcttcg agctgatcgg tcgcggacgc accacccgcc aaatcagcgg
     4441 ctatcttcat ctcgg
//
LOCUS       DASTDG010000191         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1398720, whole genome shotgun sequence.
ACCESSION   DASTDG010000191 DASTDG010000000
VERSION     DASTDG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398720"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VFO30_06060"
     CDS             complement(<1..1366)
                     /locus_tag="VFO30_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET9418887.1"
                     /translation="MTTTTLAQQLADYASSLRFEDLSAEVVREVKRRMIDSVGCALGA
                     WDEEPCRIARDVASGFSAKNGSTIIGTSHTSPPDWAAFANGCCIRYFDYNDTYLSKEP
                     AHPSDNFSAALAIAESVGASGRELITAVALAYEVQCRLCDAASIRARGWDHVTYGAFS
                     TALASTRLMLLDPARTRHAVNIAGVACAAMRQARVGELSHWKGVAFANTARHGVYSAL
                     LARAGMTGPAPIFEGQMGFEKQLGVSLGSIGENFAVPWVPSKFGPASMILNTSIKYWP
                     AEYHSQSAIEAALFLRKQIVDPAAGAVSAKEALAQIESVLIESHDAAVDIIGSEPEKW
                     KPETRETADHSLPYITAVALIDGEVTERQFQPARFTDPKIWKFLESVRVERNAELSGM
                     YPDAVANILHVNLADGRRLTKRVDYPLGHAKNPLKDSEVEGKFYSMVTPKLGKDRAQR
                     IVDSA"
     gene            complement(1491..2468)
                     /locus_tag="VFO30_06065"
     CDS             complement(1491..2468)
                     /locus_tag="VFO30_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9418888.1"
                     /translation="MAELEKGLQQPVRACELHHENWRNRTYRVELASGAVVIAKQILI
                     GTEGTVHRQADELECLSRLQVPGLHVPKPVALLPEKRVLIMEFAPGQTITALLLSRAR
                     AEEGMRACELAGAVLARLHRAWAEAVCPLPVKQLADDMAAAPWRLSRRQRETLSRTLK
                     ELGAARVSVGRIHYDYEPDNLLWDGEQLFLIDPSDGSHWGVQLFEVATFRSAVRRRLF
                     MERLRRPFGWRRGLLNRAIRGFERSYLAEGSGGDLEPRLFTLAIGFFELQRAAQLFVG
                     EKVTIDIARRKDVIGWQNEGSLINWAKLGLVEGYKCWLFSQLARELRGG"
     gene            complement(2797..>3139)
                     /locus_tag="VFO30_06070"
     CDS             complement(2797..>3139)
                     /locus_tag="VFO30_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9418889.1"
                     /translation="TTDTSFLTAYANDFGYEGLFARQVEALGKAGDLLVGISTSGNSV
                     NVMRAVESARRVDMRTVALTGGGGKLPGMVDIAVLVPSADTQHIQEAHLAIEHVICEL
                     VERHLFHREAT"
BASE COUNT          653 a          998 c          852 g          636 t
ORIGIN      
        1 acgcagaatc gacaattctt tgcgcgcgat ctttgccgag cttcggtgtg accatcgagt
       61 agaacttgcc ttccacctcc gagtccttca gcggattctt ggcatggccg agcggataat
      121 cgacgcgttt cgttaggcgc cgtccatccg cgagattgac atggagaatg ttggcgacgg
      181 cgtctggata cattccgctc aactccgcgt tccgttcgac tctcacgctc tccaaaaatt
      241 tccaaatttt cggatcggta aatcgcgccg gctgaaattg ccgctcagtt acttccccat
      301 cgatcagcgc gaccgcagtg atgtaaggca agctgtggtc cgccgtctcg cgcgtctccg
      361 gcttccattt ctccggctca ctgccaatga tgtcgacagc cgcgtcgtgt gactcgataa
      421 ggacagattc aatctgcgca agagcctcct tcgccgacac ggctccggcg gccgggtcga
      481 ctatctgctt gcgcaggaag agcgccgctt cgatggcgct ttggctgtga tattccgccg
      541 gccaatattt gatgctcgtg ttcaggatca tcgaggcggg tccaaacttc gacgggaccc
      601 acggcacggc gaagttttcg ccgatgctgc ctaacgacac cccgagctgc ttttcaaatc
      661 ccatctgccc ttcaaagatt ggcgccggtc cggtcatccc tgcacgcgcg agcagcgcgg
      721 agtaaacgcc gtgtcgcgcg gtgttggcga atgctacgcc tttccagtgc gaaagctcgc
      781 cgacgcgggc ctgtcgcatc gccgcgcatg ccactccggc aatgttgacc gcgtgccggg
      841 tgcgagcggg atcaagcagc atcaggcgcg tggaagcgag cgcggtggag aaagcgccgt
      901 aggtcacgtg gtcccagccg cgcgcgcgaa tgctggcggc atcgcacaat cgacattgca
      961 cttcataagc aagggcaacc gccgtgatca gttcgcgtcc actcgcgccg acgctctccg
     1021 cgatggccag agccgcggaa aagttgtcgc tcggatgcgc gggttctttc gaaagataag
     1081 tatcgttgta atcgaaatag cgaatgcaac agccgttcgc gaacgctgcc caatccggcg
     1141 gcgacgtgtg actggtgccg atgattgtgg atccattttt cgccgaaaat cccgacgcaa
     1201 cgtcgcgtgc aatcctgcac ggctcctcgt cccaggcacc gagcgcgcat ccaactgagt
     1261 caatcatgcg ccgcttcact tcgcgcacga cttctgccga taaatcttcg aagcgcagcg
     1321 aagacgcgta atcggccagc tgttgagcaa gcgtagtcgt ggtcatgaaa cagcaatacg
     1381 aagtctgccg ccgatttccg cgtgagcaac tcacttgcgc atgtgcacgc ccacgctcac
     1441 atggacatgc ccgcgcttag ccgagcctcg agagcggcgc gcgcgactat ttagcctccg
     1501 cgtaattccc gggcgagctg gctgaatagc cagcatttat aaccttcaac aaggcccaat
     1561 ttggcccaat tgatcaaaga accttcgttc tgccagccaa tcacatcctt ccgccgtgca
     1621 atatcgattg tcactttttc gccgacaaac agttgcgcgg cacgctgcag ttcgaaaaat
     1681 ccgatggcga gtgtgaacag ccgcggctca agatcgcccc cgctgccctc tgctaaataa
     1741 ctccgctcga acccccgaat agcccggttc agcagaccac ggcgccaacc aaacggccgc
     1801 cgcagcctct ccataaatag acgccgtcgc actgcgctgc ggaatgttgc cacctcgaat
     1861 aattgaaccc cccaatggga gccgtccgaa ggatcgatga gaaacagctg ctcgccgtcc
     1921 cacagcaggt tgtccggctc gtaatcgtaa tgaatccgcc cgacgctcac tctggcagcg
     1981 ccgagctctt tcaaggtcct gctcagcgtc tctctctgcc gtcgtgataa tcgccacggt
     2041 gcagccgcca tatcatcggc gagctgtttg actggtagcg ggcaaactgc ctccgcccag
     2101 gcacgatgca gtcgcgccaa aaccgctccg gctagttcac aagcacgcat cccctcttcg
     2161 gcgcgcgcgc gactcaaaag caatgcagtg atcgtttggc cgggtgcaaa ttccataatt
     2221 aaaacccgct tctccgggag caacgccacc ggctttggca cgtgcaaccc gggaacctgc
     2281 aaccgactta agcattccaa ttcgtcggct tgccgatgca ccgtcccctc agttccaatt
     2341 aatatttgct tggcaatgac gaccgccccg ctcgccagct ctacgcgata ggtgcggttc
     2401 cgccaattct cgtgatgcag ttcgcaagcg cgtaccggct gctgcaagcc tttctcaagc
     2461 tcagccaccg taggtggact caccacatct gatcggctgg cacgtagccg ttccaatcgc
     2521 tcgtggaatg cagcgatgtg accgcactgc atcagcgaag atgagtcaat ggcaccgagc
     2581 gaagcaggcg agcgtgaacc tgtgttcgag aattggactg caccagcaaa cgcccgattc
     2641 atagtgtcaa tgcgacaggt tactgaaaac gtttgccgct attcaagccg gcagttgtat
     2701 gcggcatatc ccgacagatg gcgctacgct gtaccgtgcg tcgaagaaga gacgctgaag
     2761 agtcctgcga cgatcataag tctctaagtc tcagacctac gtggcctcac ggtgaaacag
     2821 gtgccgctcg accagctcgc agattacgtg ctcaatcgcg aggtgggcct cctggatgtg
     2881 ttgggtgtcg gcgcttggaa ccaagacggc gatgtcgacc atccccggca attttccgcc
     2941 gccgccggtc agcgcaaccg ttcgcatgtc gacacgacgc gccgactcga ccgcgcgcat
     3001 cacgttcact gagttcccgc tggtactgat cccaacgagc aggtctccgg ccttacccaa
     3061 cgcctccacc tgtcgcgcga aaaggccctc gtaaccgaag tcgttcgcat aagcagtgag
     3121 gaaggacgtg tcggtcgtc
//
LOCUS       DASTDG010000192        10826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_426569, whole genome shotgun sequence.
ACCESSION   DASTDG010000192 DASTDG010000000
VERSION     DASTDG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426569"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VFO30_06075"
     CDS             complement(<1..1441)
                     /locus_tag="VFO30_06075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9418890.1"
                     /translation="MAVREVAGLIAVTMLTSANLKAAPPEDSTVVKFPPSTAEKWVLP
                     DGLTIIVQEDHSAPVASVQAWCATGSVNEDEHLGAGLSHILEHMLFKGTKTRSTNAIA
                     QAIQNVGGYINAYTSFDRTVFWIDVPKAGVATALDVLSDAMMNSTLPPSEYAKEQEVI
                     RREFAMNRDDPDRVTGQLLFATAYQKHPYRLPVIGEMEIYNQLTQEQVMQYYKTRYVP
                     NNLTLVVVGDVDAEKVRAQLAKFFEAYPEKSLKPAFIPPEPPQLGRREVHNEFVTELT
                     RLSLAWHIPEVTHPDVPAIDLLSGILGDGRSSRLYRKVREESGLAYSVSAFSYTPGDP
                     GLFGIDATVDPKKREAAEQLILQLVDQVRHDGVTAEELTKAKKTSLSHHLHALTTMRG
                     QASDVGSNWLLTRNLNFSREYLAAVQKVTLNDIRRVCATYLSEQNLTVVSLNPKGSLA
                     GKTETAKPVAAAEIQKFELANGLRILVRED"
     gene            complement(1490..1927)
                     /locus_tag="VFO30_06080"
     CDS             complement(1490..1927)
                     /locus_tag="VFO30_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HET9418891.1"
                     /translation="MQKIGFAEALDSILASDRRYQRDAYVFLRDALDFTTKQQKKIKG
                     ATVRHVAGPELLDGVRQYALKEYGPMVMTVFDAWGIRCCEDIGNMVFNLIAAGIFGKT
                     EEDSIEDFKNVFAFEDVFVKPFTPMKAISVKPPAPGLPAPKSV"
     gene            2188..2574
                     /locus_tag="VFO30_06085"
     CDS             2188..2574
                     /locus_tag="VFO30_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HET9418892.1"
                     /translation="MSSETTKSQQSPRITHISWGRMEIENLGTGRDFKLYPGGGRAWD
                     WNETGTHHVPGIQPADVQELLDHGSRVVVLSRGMALALQTCPETLQLLRDQGITYHVQ
                     ETSAAVELYNRLAQTEPVGGLFHSTC"
     gene            complement(2674..3948)
                     /locus_tag="VFO30_06090"
     CDS             complement(2674..3948)
                     /locus_tag="VFO30_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418893.1"
                     /translation="MKQPTLVAAMISTLLLCRVASGAPVERTVSPSRQFIIYGADARV
                     RGAISELAERTKKDLLALLHQTDGWIMPVLINLQLPQTTRAELPAAAFHVSQTGFGLK
                     LQLDLMLASNVDVTTVERELLRALVVEMIYRNQPNVAPGTVLAQPPPWLLEGILAAAP
                     GQDRAPLVEALLSSREPISLQQFLQQNPALLDSPARMLYRAYSLALVQLLLASDDGTA
                     RLSGFVANLTHASNDPLADLKARFPALGDEPEKTWRAHLARVCAVEGPRLFSFAQTQR
                     GLEELLQGNGGSATETQLAQFSQKKISAADKAALAALRQNLLELTVHANPLLRPVVQE
                     YEQITVLLLTGKHRGIAQRLAQLKSMRTRLAARMRNIDDYMNWFEATQSQTKSGVFVD
                     YLRTATKPGTPEPRRRDPLSIYLDAVEQQTQN"
     gene            4036..4548
                     /locus_tag="VFO30_06095"
     CDS             4036..4548
                     /locus_tag="VFO30_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9418894.1"
                     /translation="MNFRRLLPVIAAVTIAGAMPTAQAGYRYYYPYRHHYHSYTNVSI
                     GFGVGYPYGGWGYGWPYSSYGYYPYGYGYGYGYYPSAYSYYPSTYGYYPYSYGYYSRP
                     TYRGQSVNSNVVVRVQERLARSGYYHGAIDGVAGPATRQAIRAYERTHGLRVDGVVSN
                     QLLATMGLRY"
     gene            complement(4624..5382)
                     /locus_tag="VFO30_06100"
     CDS             complement(4624..5382)
                     /locus_tag="VFO30_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9418895.1"
                     /translation="MMTKLVCPECGHDNEPERVYCHSCGARLDRSGVAVGKSREEAFH
                     ETRRRVRSMFDPTGVKIRFWFFRTAKLILAACAAAAVIEMISPPDVPPPVKSSLLLSQ
                     IGLELENAATYHRPPQLQYSEDQVNAHLAYALRNKQSTLDKPLLEFKRAIVGVEEGRL
                     KLTTERSLLGLSLYSSVSYLVTLRQGHITATSNGGGIGRVPIHPQLMRFVDGIFGDVW
                     TALDRERKLVAKMGSIELHSKRVVLNVAAPAAVQ"
     gene            complement(5379..5726)
                     /locus_tag="VFO30_06105"
     CDS             complement(5379..5726)
                     /locus_tag="VFO30_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET9418896.1"
                     /translation="MPTYEYRCTKCGQNFEAFQSMRAAPFRECPKELCRLARWGRGKV
                     KRLLGTGAGIIFKGSGFYSTDYRSKSYQEAAKKEAPQAASTDKPSPAKDSPTKPAPAT
                     STTTRNSPKIGQP"
     gene            5885..6496
                     /locus_tag="VFO30_06110"
     CDS             5885..6496
                     /locus_tag="VFO30_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9418897.1"
                     /translation="MFRFFCKHVLVLLLSATVVFLVGCAQDVGYSGVTQYLGGIYGNA
                     PTVGAPQDTVSYWDGDGIGGKSSIKITLREQRAYFYKSGLLVGVSQLSTGREGLNTPI
                     GHFSIIQKDLKHVSTKYGDYVDDAGNVVVENVELGKDPKPPGTHFKGAPMPYFMRIVD
                     GVGLHAGYLPGYPASHGCIRMPEFMAENFFKSVSTGTPVTITN"
     gene            6614..9838
                     /gene="mfd"
                     /locus_tag="VFO30_06115"
     CDS             6614..9838
                     /gene="mfd"
                     /locus_tag="VFO30_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9418898.1"
                     /translation="MKRSAELLERVVRSEPIARKLQLIVSAEWPVKFSHVSEPAQPFL
                     TAALASKIDKTIWILCPTIHSQELFYDSLLNWTPTALFLPETELGAVENVLPDPEITA
                     ERLALFSLLESERSSKLIVATRASLQQPAPKPGLLAAGFMPLRRGTAQSMDDLLNRLA
                     SAGYERVPQVTTRGHFAVRGGIVDLFSWQRALPVRVEFFGDAVESLREFDIDTQTSVR
                     ELSAADILLGAPEEQTGKVADYVAADHFTIDIEPEADSSAQLQISEGWIEQGVEDFSG
                     ALQDCELGEFAIGDLAIAEAKRAQFLTRVEQWRSAQAKIVIYFQTEGEIERFRELLGG
                     ERALADVDLVEGMLARGFCFPAANLVVLSAAELFGRFAAHTRRRLRRAERHRAQIDFS
                     ELAQGDLVVHLEHGVGRFLGLRKFVVGQAPHLPDADGTKLASTALALQAQQQPQEVLV
                     LEFADQAKLYVPLEQAYLVSRYVGVGKKSPPLSSLGDAKWARAKRNAAASVFDYAGKM
                     LTVQAERETSPGHAFGPDTKWQTEFERSFPFRETPDQLKAIVEAKIDMEKPRPMDRLI
                     CGDVGFGKTEVAIRAAFKAVMDGKQVAVLAPTTVLAQQHFEVFRQRMLDYPVRIEMLS
                     RFRTQSEQNKILQLLREGGVDIVIGTHRLISGDLVFRDLGLVVIDEEQRFGVLHKEKF
                     KELFKLVDVLTLSATPIPRTLYLSLVGVKDMSTIETPPLNRLPVETVVCAYDERIIRD
                     AIKRELERQGQVFFLHNRVQTIERARDRIVDLCPEARVEIGHGQMDADKLEAVMARFV
                     AGKTDVLVCTTIIESGLDIPNANTIIIDRADRFGLADLYQLRGRVGRAEHKAYAYLLL
                     PRELMTVGAARKRINAIKQYSSLGAGFRIAMRDLEIRGAGSILGTAQSGHIMAVGFEL
                     YCQLLKQAVAQRKGEKLRLRLDVDLRLDFVATNEAEFAQLGPERCVPAFIPSSFIAET
                     TLRIQAYRHIAEITTPEQLQRLRREWRDRFGAFPSAAENLFLLAEIKLAAAKSDISRV
                     EVREDKLMLTRRGDFILVAGKFPRLVAAGLAQRLREILELINKV"
     gene            9835..10824
                     /locus_tag="VFO30_06120"
     CDS             9835..10824
                     /locus_tag="VFO30_06120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9418899.1"
                     /translation="MKRPLNGIFVAALLLWGFALLPLCRAAYAAEAEAVDGIAAIVNG
                     DVITYSQVRAVSATREKVLHAQYTGDELVNQIKEVRKAALQDLIDRQLILQAFKKEKY
                     EIPDHFVDERMHEIIRESFGGDRNTFVKTLEAQNYTLGEFKKLETERMIVQAMRSKNV
                     KRNMAVSPTKIEDYYRTHRDEFTSKEEIKLRMIMIPSRADTGNAPAQKAMVEEILGKL
                     ASGASFERLAQIYSEDSTRELGGDWGWIERKTLAVPLEQLAFNLPVGRISRIIDYNGN
                     YYILKVEDKRGGETKPLAQVRDEIEKNLTQLEAQQLQEKWLASLRSKAYIRTF"
BASE COUNT         2269 a         3148 c         3164 g         2245 t
ORIGIN      
        1 gatcctcgcg cacgagaatg cgcaggccgt ttgccaactc gaatttctga atctcggccg
       61 ccgcgaccgg ttttgccgtt tccgttttgc cggcgagcga tcctttcgga ttcagcgaga
      121 cgaccgtgag gttttgctcg ctgagataag tcgcgcagac gcggcggatg tcgttgaggg
      181 tcactttctg gacggcggcg aggtactcgc gactgaaatt caaattgcgc gtgagcagcc
      241 aattcgaacc aacgtccgat gcctggccgc gcatggtggt gagcgcgtgc agatgatgac
      301 tgagcgaagt ctttttcgcc ttggtcaatt cctccgcagt cacgccgtcg tgtcttactt
      361 gatcgacaag ttgcagaatc aactgctcgg ccgcttcgcg ctttttagga tcgacagtcg
      421 catcgatccc gaacaagccg ggatcccccg gcgtgtagga aaacgccgag acgctgtaag
      481 cgagtccgga ttcctcgcga acttttcggt agagacgcga gctgcgtcca tcgccgagaa
      541 ttccggagag caaatcaatc gccggcacgt cggggtgcgt aacctcggga atgtgccagg
      601 cgagcgagag ccgcgtcagc tccgtgacaa actcattgtg tacttcgcgc cgaccgagct
      661 gcggcggttc cggtgggatg aacgccggct tcagcgattt ttcgggataa gcttcaaaaa
      721 acttggcgag ttgcgcgcgc actttctccg cgtccacatc gccgacgaca accaaggtga
      781 gattgttcgg gacatagcgc gtcttgtagt actgcatcac ctgctcttgc gtgagctgat
      841 tgtaaatctc catctcacca atgaccggca ggcggtaggg atgcttttgg taagcagtgg
      901 caaacaggag ttggcccgtc actcgatcgg gatcatcgcg gttcatcgcg aactcgcggc
      961 gaatcacctc ctgctccttc gcatattccg agggcggtag ggtcgaattc atcatcgcgt
     1021 ccgacaaaac gtcgagagcg gtcgcgacgc ctgcttttgg cacgtcgatc caaaacacgg
     1081 tgcgatcgaa cgaggtataa gcgttgatgt aaccgccgac gttctgaatg gcctgcgcaa
     1141 tcgcattggt cgatctcgtc ttcgtgccct tgaagagcat gtgctcgaga atgtgcgaga
     1201 gacccgcgcc aagatgctcg tcttcattga cgctgccggt ggcgcaccaa gcctgcacgc
     1261 ttgccaccgg cgcgctgtga tcttcctgca cgatgatcgt cagcccgtcg ggcagcaccc
     1321 atttctccgc cgtgctcggc ggaaacttca cgaccgtgga atcttccggc ggcgccgctt
     1381 tcaaattcgc actggtgagc atggttacgg caattagtcc cgctacttcg cgaacagcca
     1441 tgaatctgtc aatgatcccc gcttcaagaa agtagcgggg caaattgcct cataccgatt
     1501 tgggcgcggg aagtccgggc gccggcggct taaccgaaat tgctttcatc ggtgtgaacg
     1561 gctttacgaa tacgtcctcg aacgcgaaca cgtttttgaa gtcttcgatc gaatcttcct
     1621 ccgtcttccc gaaaatcccg gccgcgatca ggttaaaaac catgttgccg atgtcttcgc
     1681 aacagcggat tccccacgcg tcgaagaccg tcatcaccat cggcccgtat tccttcagcg
     1741 cgtactgccg cacaccgtcg agcaactccg gcccagcgac atggcgcacc gtcgcccctt
     1801 tgatcttctt ttgctgctta gtcgtgaaat cgagcgcgtc gcgcaaaaaa acgtaggcat
     1861 cgcgctgata acgccggtcg ctcgccagaa tcgagtcgag tgcttcggca aatccaatct
     1921 tctgcatgaa tcaacagcgc gcgtcttggc gcaccgttta ccataagcag cgacccgatg
     1981 cgcggcaact gttcgcaaag actgatcgaa gaattaggaa gtagaaagta caagatttca
     2041 tggccgtttg cgaaaccagc tggatgcagg agcttgagct cgttaggccg ttagcatgcg
     2101 caaactgatc tccagtggca gtccgtacga accggattgg ctcgtggagt tcgaagccga
     2161 cgctgttgtc gatgacgagg gactttcgtg agcagcgaaa caacaaagtc ccagcagtcg
     2221 ccgcgcatca cgcatatctc gtgggggcgc atggaaatag agaacctcgg cacgggacgc
     2281 gatttcaaac tttacccggg tggcggacgg gcgtgggatt ggaatgagac cgggacccat
     2341 catgtccccg gcattcagcc agccgacgtg caggagttgc tcgaccatgg cagcagagtc
     2401 gtcgttctca gccgcgggat ggcattggcg ctgcaaacct gtccagagac actgcaactg
     2461 ctgcgtgacc aaggcatcac ctatcacgtc caggagacga gtgcggcggt agagctttac
     2521 aatcgcttgg cgcaaacgga gccggtcggc ggcttgtttc attcaacctg ctgaccgcgt
     2581 tcagcgcgtt gctatcgaaa tacagcgcga cgaaacggat cccgaacagg ggctgaaaaa
     2641 ttctctgtgc cggcaagcat gaagctcagg ccttcagttt tgcgtttgtt gttccaccgc
     2701 gtcgaggtaa atcgagagtg ggtcgcgccg gcgcggttcc ggtgtccccg gtttggtggc
     2761 ggtgcgtaga taatcgacaa acacgccgct tttggtttgg gactgcgtcg cttcgaacca
     2821 gttcatgtaa tcgtcgatgt tgcgcatgcg cgcggccagg cgggtgcgca tcgacttcaa
     2881 ttgcgcgagt cgctgcgcga ttccgcgatg ttttcccgtg aggagaagaa cggtgatttg
     2941 ttcgtattcc tgcacgaccg gtcgtaacaa cgggtttgcg tgaaccgtta actcaagcag
     3001 gttttgcctg agcgccgcca gcgcggcttt gtccgccgcg gagattttct tttgcgaaaa
     3061 ttgcgccagc tgtgtttctg tcgcagagcc gccgtttcct tgcagcagtt cctcgagtcc
     3121 gcgctgcgtt tgggcaaaac tgaacagccg gggaccttcg accgcgcaaa cccgcgccag
     3181 atgcgcgcgc cacgttttct ccggctcgtc gccgagcgcg ggaaaccgcg cctttaaatc
     3241 cgcgagcgga tcgttggagg cgtgggtcag attcgcgacg aaaccgctga gccgcgccgt
     3301 cccatcgtcc gacgcgagca atagttgcac gagcgccagt gagtaggcgc ggtatagcat
     3361 gcgggcgggc gagtcgagca gcgccgggtt ttgctggaga aactgctgca gtgaaatcgg
     3421 ctcgcgtgag gaaagtaatg cttcgacgag cggcgcacga tcttgccccg gcgccgcggc
     3481 caagatgccc tcaagcaacc acggcggcgg ctgcgctagc acggtgccgg gcgcaacgtt
     3541 cggctgattt cgatagatca tctccacaac cagcgcacgc agcagttccc gctcgacagt
     3601 tgtaacatcg acattggacg cgagcatcag atccagctgg agcttgaggc cgaatccggt
     3661 ctggctgaca tgaaatgccg ccgctggcag ctccgctctt gtcgtttgcg gtaactgcaa
     3721 attgatgagc acgggcatga tccagccgtc agtctgatgc aggagggcga gcaaatcttt
     3781 cttggtacgt tccgccagct cggaaatcgc gccgcgcacg cgagcatccg caccgtaaat
     3841 gatgaactgg cgcgacgggc tgacggtgcg ctccaccggc gcaccagacg cgacgcggca
     3901 cagaagcagg gtcgatatca ttgctgcaac aagcgtcggc tgtttcattt aataggcgat
     3961 aatgttctgc cggaaagctc ggaaaaacgc aacttccgaa acttggcggt gtgtaatcag
     4021 tgaagagaca ggcttatgaa ctttagacgc ttacttccgg tgatcgccgc ggtgacgatc
     4081 gcgggtgcaa tgccgaccgc gcaggcgggc taccgttact actaccctta tcggcatcat
     4141 tatcattcct acaccaacgt ctcgattggc tttggggtcg gctacccgta cggggggtgg
     4201 ggatacggct ggccctacag ttcctatggc tactatccct acggctatgg atatggttat
     4261 ggatactatc cctcggccta cagctactat ccttccacct atggctatta cccttacagc
     4321 tacggatatt actcgcgtcc aacttaccga gggcagtcgg tcaacagtaa tgtcgtggtt
     4381 cgcgtccagg aaagattagc gcgcagcggc tactatcatg gtgcaatcga tggggtggct
     4441 ggaccggcga cacgacaggc aatccgcgct tatgaacgca cgcacggtct gcgcgttgat
     4501 ggcgtggtta gcaatcagct tcttgcaaca atgggtctgc gttactgagg ctatcattct
     4561 cgatctttcg gttggtcggc cggtaggccc gcatggtgag gagggaaggg tctgccggcc
     4621 gttttattgc accgcggccg gtgcggcgac gttgaggacg actcttttag aatgcagttc
     4681 gatgctcccc attttggcca ccaacttgcg ctcccggtcc agtgccgtcc acacatcacc
     4741 gaaaatgccg tcaacgaatc gcatcaattg cggatgaatc ggcacgcggc cgatgccgcc
     4801 gccattgctc gtagcagtga tgtgcccctg ccgcagcgtc accaaataag acacgctgct
     4861 gtagagcgag agcccaagta acgaccgctc ggtcgtaagt ttgagccgcc cctcttccac
     4921 ccctacgatc gcgcgcttga attccagcag cggtttgtcc agcgtcgatt gcttgttcct
     4981 caacgcgtaa gccaggtgag cgttcacctg gtcctcgctg tattgaagct gcggcggccg
     5041 gtgataagtg gcggcgtttt ccagctccag cccgatctgc gaaagaagga gcgacgattt
     5101 caccggcggc ggcacatccg gcggggagat catctcgatg actgctgccg ccgcgcacgc
     5161 cgcgagaatt aatttcgccg tgcggaaaaa ccaaaaccgg attttcaccc cggttggatc
     5221 aaacatgctc cgaacccggc ggcgcgtttc atggaaggct tcttcgcgtg acttgccgac
     5281 cgctacaccc gagcgatcga ggcgcgcacc gcagctgtga cagtagacgc gctcgggctc
     5341 gttatcgtgt ccgcactcgg gacaaaccag cttagtcatc atggctgacc gattttgggg
     5401 gagttgcgag tcgttgtcga cgttgccggc gcaggtttgg tcggcgaatc cttggccggc
     5461 gaaggtttgt cggtgctcgc agcctgaggc gcttctttct tggccgcttc ctggtacgac
     5521 ttgctccggt aatccgtact atagaagccg ctgcctttga aaataatgcc ggcaccggtg
     5581 ccgagcagtc gtttcacttt accgcgaccc cagcgtgcca gccgacaaag ttctttcgga
     5641 cactcacgaa agggcgccgc gcgcatcgac tgaaatgctt caaaattctg tccgcatttc
     5701 gtgcagcgat attcgtaagt aggcatggca aaacaaagcc gcggcagtgt gcatgcagca
     5761 aagcacaatt caagccgcac gggcgcgcag gccactgaac tggcgccgcc gcggacgtag
     5821 attgtgcgtc tcttgacttt gcccgttccg ccggtacaac gtgcgtcccc gaatagtttt
     5881 cacgatgttt cgattctttt gtaagcacgt tttggttctc cttctatcgg cgacagttgt
     5941 gtttctcgtc ggttgtgcgc aggacgttgg ttactcgggc gtgacccagt acctcggcgg
     6001 gatttatggc aacgcgccga ccgttggggc gccgcaagac acagtctcct actgggatgg
     6061 ggacggaatc ggcggcaaat catcaattaa aatcactctg cgggagcagc gcgcctattt
     6121 ttacaagagc ggactgctgg tcggcgtgtc ccaactttcc actggacgcg aaggactgaa
     6181 cacgccgatc ggccatttct ccattatcca aaaagacctc aaacacgtct cgacaaagta
     6241 cggcgactac gtcgatgacg ccggaaacgt ggtggtggaa aacgtcgagc tcggcaagga
     6301 cccgaagccg cccggcacac atttcaaagg cgcgcccatg ccctacttca tgcgcattgt
     6361 cgatggagta ggactgcacg ccggctattt gccaggctat cccgcttcgc acggctgcat
     6421 ccgtatgccg gagttcatgg cggaaaattt cttcaaatcc gtttccacgg gcacgccggt
     6481 cacgattact aactaagctg gatcaagctt tccgagctcg atgcggcccg cagcacgaaa
     6541 ccgcaccctt aaagtcgccc gcgcaacggc gatccatttt tgaaggttcg cctgctctcg
     6601 gcgaacattg atcgtgaaaa gatcggccga gttactcgag cgcgtggtgc ggtcggagcc
     6661 aatcgccaga aagctccaac taattgtcag cgccgaatgg ccagtaaagt tctcgcatgt
     6721 gagtgagccg gcacagccgt ttctcaccgc cgcgctcgca tccaagatcg acaaaacgat
     6781 ctggattttg tgcccgacca ttcattcaca ggaactgttt tacgacagtt tgctcaattg
     6841 gacgccgacc gccctgttcc tgcccgagac cgaattgggg gcagtggaga acgtgttgcc
     6901 cgaccctgaa atcacggccg aacggctcgc gttgttttca ctgcttgaga gcgaacgcag
     6961 ctccaaactg atcgtcgcga cgcgcgcgag tctgcagcaa ccggcgccaa aacccggcct
     7021 gctggcggct ggcttcatgc cgctgcggcg cggtacggcg caaagcatgg acgacctcct
     7081 caaccgactc gccagcgccg gctacgaacg ggtgccacaa gtcacgacgc gcggccattt
     7141 cgccgtgcgc ggcggaattg tcgatctttt ctcctggcag cgcgcattgc cagtgcgcgt
     7201 ggagttcttc ggtgacgccg tcgaatcgct gcgcgaattc gacatcgata cacaaacctc
     7261 cgtccgcgaa ttgtccgccg ccgatatttt gctcggggcg ccggaggagc aaaccggcaa
     7321 agtcgccgat tacgtcgcgg ccgatcactt tacgatcgac atcgaacctg aggcggattc
     7381 atccgcgcaa cttcaaatca gcgaaggctg gatcgaacaa ggcgtagagg atttcagcgg
     7441 cgcgcttcag gattgtgaac tgggcgaatt tgcaataggc gacctcgcca tagccgaagc
     7501 caaacgcgcg caatttctca ctcgggtcga gcaatggcga tccgcccagg caaagatcgt
     7561 catctatttt caaaccgaag gcgagattga gcgcttccgc gagctgctgg gcggagaacg
     7621 cgcgctcgca gatgtcgatc tcgtcgaagg catgctggcg cgtggttttt gttttccggc
     7681 ggccaacctg gtcgtccttt cggccgccga gctattcggg cgttttgccg cccacacgcg
     7741 gcggcgattg cgtcgggccg aacggcaccg cgcgcaaatc gacttcagtg aactggccca
     7801 aggcgacctc gtcgtccacc tcgaacacgg ggttggcagg ttcttggggt tacgaaaatt
     7861 cgttgtcggt caggcgcccc acctgccaga tgctgacgga acaaaattgg caagcacagc
     7921 gcttgccctg caagcgcaac aacagccaca ggaagttctt gtgctcgagt tcgccgatca
     7981 ggcgaaactg tatgtgccgc tcgagcaggc ctacctcgtt tcacgctacg ttggggtcgg
     8041 caagaaatcg ccgcccctca gttcgttagg cgacgcgaaa tgggcgcgtg cgaagagaaa
     8101 tgccgccgct tccgttttcg attacgccgg taaaatgctc accgtccaag ccgagcggga
     8161 aacttctccc gggcacgcct tcggtcccga tacgaagtgg caaacagaat tcgagcgttc
     8221 cttccccttt cgggagacgc ccgaccagct gaaggcgatc gtcgaagcca agatcgacat
     8281 ggagaaaccg cggccgatgg accgattgat ctgcggcgat gtcggttttg gcaaaactga
     8341 agtggcgatc cgcgcggcgt ttaaagcggt gatggacggc aaacaagtcg ccgtgctcgc
     8401 acccacgacc gtgctcgcgc agcagcactt cgaagtcttt cgccagcgca tgctcgacta
     8461 tccggtgcgt attgagatgc tcagccgctt ccgcactcag tctgagcaga acaaaattct
     8521 gcagctgttg cgcgaaggcg gcgtggacat cgtgatcggc acgcaccggc tcatctcggg
     8581 tgatctggtc ttcagagatc ttgggctggt tgtaatcgat gaggagcagc gcttcggtgt
     8641 tttgcacaag gagaagttca aagagctgtt caaactcgtt gatgtgctca cgctctcggc
     8701 cacgccgatc ccgcggacgc tctatctctc gctcgtcggc gtcaaagaca tgtcaaccat
     8761 cgaaacgccg ccgctgaatc gactgcccgt ggagacggtc gtctgcgcgt atgacgaacg
     8821 gattatccgc gacgcgatca agcgcgagct cgagcgccag ggacaggttt tctttttgca
     8881 caatcgcgtg caaacgatcg agcgtgcgcg cgatcgcatt gtcgatcttt gtccggaagc
     8941 gcgcgtcgaa atcgggcacg gccaaatgga cgcggacaag ctcgaagcgg tgatggcgcg
     9001 gttcgtcgct ggaaaaaccg acgtgctcgt ttgcaccaca atcatcgaga gcggtctgga
     9061 tattccgaat gccaatacga tcatcatcga ccgcgccgac cgtttcggac tcgcagatct
     9121 gtaccaactg cgcggtcgcg tcggccgcgc cgagcacaaa gcgtacgctt acctcttgtt
     9181 gccgcgggag ctcatgaccg tgggcgcggc ccgcaaacga atcaacgcga tcaagcaata
     9241 cagctcgtta ggcgcgggat tccgcattgc catgcgcgat ttggagatcc gcggcgcggg
     9301 cagcattctc ggcaccgccc aaagcgggca tatcatggct gtcgggttcg agttgtattg
     9361 ccagttgctc aagcaagcgg tcgcgcagcg gaaaggcgaa aaactgcggc tccgcttgga
     9421 tgtcgatctt cgcctcgact ttgtcgcaac caatgaagcc gagttcgctc agctcggacc
     9481 cgaacgttgc gtgccggcgt tcatcccgag cagctttatc gctgaaacca ccttgcggat
     9541 tcaagcttat cgccatatcg ccgaaatcac cacgcccgag caactgcagc gactgcggcg
     9601 ggagtggcgt gaccgtttcg gcgcgtttcc gagcgcggct gagaacctgt ttctcctcgc
     9661 cgagataaag ctcgccgcgg cgaagagcga tatcagtcgt gtggaagtgc gcgaagacaa
     9721 gttgatgctg acccggcgcg gggattttat acttgtggca ggcaaattcc ctcgtttagt
     9781 tgcggcggga ctcgcacagc gcctgaggga aattctggag ctgatcaaca aagtatgaag
     9841 cgccccctga acggaatttt cgtcgcggct ctgctcctct ggggctttgc gctgctgccg
     9901 ctttgtcgcg cggcgtatgc ggccgaggcg gaagcggtcg atggtatcgc cgcgatcgtc
     9961 aatggggacg tcatcactta ctcgcaggtg cgcgccgtct ccgcgacgcg cgaaaaagtt
    10021 ttgcacgccc aatacaccgg cgacgagctc gtcaaccaga tcaaggaagt ccgaaaggcc
    10081 gcgctgcagg atttgattga tcggcagctc atcctgcagg catttaaaaa agagaagtac
    10141 gagattcccg accattttgt cgacgaacgc atgcacgaaa tcatccgcga atcgttcggc
    10201 ggtgaccgca atacgtttgt caaaacgctc gaggcgcaaa attacaccct cggtgaattc
    10261 aagaagctgg agacggagcg gatgattgtg caggcgatgc ggagcaagaa tgtcaagcgc
    10321 aacatggccg tctcaccaac caagatcgaa gactattacc gcacccaccg cgacgaattc
    10381 accagcaagg aagagatcaa attgcgcatg atcatgattc cgagccgcgc cgataccggc
    10441 aacgcccccg cgcagaaagc gatggtcgaa gaaatcctcg gcaaacttgc tagcggcgcg
    10501 agcttcgagc gactggcaca gatttattcg gaggacagca cgcgcgagct cggcggtgac
    10561 tggggctgga tcgagcggaa aacgttggcc gtgccgctgg aacaattggc gtttaatctg
    10621 ccagttggcc gtatcagtcg catcatcgac tacaacggca actactacat tttaaaagtc
    10681 gaagacaaac gtggcggcga gaccaagcca ctcgcgcagg tgcgcgacga gatcgaaaaa
    10741 aacctgacgc aactcgaagc gcagcagctt caggaaaaat ggctcgccag cctgcggtcg
    10801 aaagcctaca tccgcacgtt ctgatc
//
LOCUS       DASTDG010000193         5889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1394746, whole genome shotgun sequence.
ACCESSION   DASTDG010000193 DASTDG010000000
VERSION     DASTDG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394746"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFO30_06125"
     CDS             <1..838
                     /locus_tag="VFO30_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET9418900.1"
                     /translation="LTRERSQLDLTDTKAVNDLFAKEKPSVVVLAAAKVGGIKANNDF
                     PVEFLLENLRIQNNVIRAAHDAGARKLLFLGSSCIYPKHAPQPIPESALLTGPLEPTN
                     EAYAVAKIAGIKLCQAFSREYGDNFISVMPTNLYGPNDNFDLETSHVLAALLRKAHEA
                     KQRKERELVVWGSGTPRREFLHVDDCAAACVFLLENYDSPEIINVGCGEDVSVRELAE
                     LICDIVGFEGELTWDMTKPDGTPRKLLDVTRLHKLGWRHTISLRDGIARTYEWFVKNA
                     AR"
     gene            843..1763
                     /locus_tag="VFO30_06130"
     CDS             843..1763
                     /locus_tag="VFO30_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9418901.1"
                     /translation="MKELRVGVVGVGHIGSNHARLYAEIPNAQFAAIYDVDLAKAKTI
                     GKKYRACVAESLDEFLEAVDAASIATPTSTHYEIARALLARGKHLLIEKPITDNTEQA
                     TELCELASRSGLILQVGHVERFNPVLSALEQRLTHARFIEAHRLSPYPERSTDIGVVL
                     DLMIHDLEVILHLVRSPVQNIDAVGVPVLSRSEDIANARLRFKNGCVANITSSRISPE
                     RMRKIRVFQEDAYLSLDYETQSGEIYRRADGHITRDKVEIAREEPLKSQLVSFVECAK
                     TGRQPRVSGFQATAALELAVEITKRIAASG"
     gene            1756..2883
                     /gene="lpxB"
                     /locus_tag="VFO30_06135"
     CDS             1756..2883
                     /gene="lpxB"
                     /locus_tag="VFO30_06135"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET9418902.1"
                     /translation="MGKTLYFVAGEASADNHGADLMKAVHALNADVTFIGRGGPHMRA
                     VAGQGLENWIAHAGVLGLWEVLKQYGYFREKFRETLREVEKSRPDAVILIDYPGFNLR
                     LARTLRRQHVARKIVYYISPQVWAWNRRRITKMARWIDLMLCIFPFEAELYNKSGLRT
                     VFVGHPMIDRLRARNVDIRRDSNLIGLFPGSRAREVRKIFPLLVATARELRRANPTLR
                     FEISAASTDLAARIQSVLDRHPQDRQFFAIKVADAPSTMQRAHVGIIASGSATLEAAY
                     FRLPFVLVYKVTWLTYLAARLLVRVKFLGMPNVLANKEIVPEFIQHEARAGAIARAVL
                     RLINNEVARERMISEFNRIIASLGEGGASERAARAILAEIR"
     gene            2883..4358
                     /locus_tag="VFO30_06140"
     CDS             2883..4358
                     /locus_tag="VFO30_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418903.1"
                     /translation="MRNFSAGELFRSKRTFFAVAGLTVLLFCAANLPWQLDDYDQAKQ
                     AFTSFEMIKEGHWLYQHTPNGRVATKPPLVGWTSAALFVVTRSWGAAWRLPSLLAAIA
                     LATLLTRVATSAYGRAAGLVAFGAFSWNLFTPRLATLVRTDMPLALTVFLIGTVIWEK
                     IRNEEPCKRSEQLSIFALLTAALLIKGPIVYAFLLPGVAAFAYCRGSDRVASAWCGWL
                     PWTAPLGVFLVWLVGGIRSESRFFDEVVRREFLARFGETIHHPQPLLFYLPHLLHKFF
                     PWSALMLALGVVALGLHRWNLRSAFRQTSPSTLWLVCWSLGGLIFMSFVPSKRVDRIF
                     PMVPPLCLLLASQLARVGSEDKARDRVYRWSAAALLLSILFAGGYSVAKVVTGYHEHR
                     DALAAFGREIRNQAAANHWRYEVIRGRDEAILLYLEKTHFAEPAKAIRQWNVGQLDAL
                     VIPDDQLPQFLARLRDVGSPSMQSGRRKNVDRPNYMLLTRD"
     gene            4407..4946
                     /locus_tag="VFO30_06145"
     CDS             4407..4946
                     /locus_tag="VFO30_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9418904.1"
                     /translation="MADEEFPKRAIETLWAPWRVDYFEKQPRDRNFLSAAARASDDAA
                     HLVITRRKTAFLMMNRYPYAVGHLMAVPYRKTADLSSLGENEIIELWELVVHAQRLLR
                     VAAKAQGFNIGLNIGECAGAGVVDHLHLHIVPRWSGDTNFMPMISGTRAISEGLCGLY
                     DKLIAAQAEVENSRRRTST"
     gene            4943..5584
                     /locus_tag="VFO30_06150"
     CDS             4943..5584
                     /locus_tag="VFO30_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418905.1"
                     /translation="MTTWIEPPPPQRKGLGCFAKGCLILLAFVILLGAAFAAGTYFAV
                     RFLRGQYFPAQHIQLPASTATPEQQQVVRARWDAFEATARARQPARIELTADDLNALI
                     ASEPRLRGKAQVSIEDHVGHLRVSVPLDAVPWLRGHYVNAECTVQSAADGNPADARVS
                     SIVVNGRPMGEELLGWQWRSWSLRRYMSDWSNERNLDRFEITAGKVILETKGG"
     gene            complement(5656..>5889)
                     /locus_tag="VFO30_06155"
     CDS             complement(5656..>5889)
                     /locus_tag="VFO30_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418906.1"
                     /translation="GPDFVLSTWVGGLKRALAVALKTQKLWQPGFFDHVLRSADSYGE
                     KWNYVRDNPVRAGLVRDANDWRYQGEVVLIDRP"
BASE COUNT         1184 a         1660 c         1778 g         1267 t
ORIGIN      
        1 actcacccgc gagcgttccc agctggatct taccgacacg aaagcggtga acgacttgtt
       61 tgcaaaagaa aaaccgagcg tggtcgttct cgccgccgca aaagtcggcg gcattaaggc
      121 gaacaacgat tttccggtcg agtttttgct cgagaatctg cgcatccaga acaacgtcat
      181 ccgcgcagcg cacgatgcgg gcgcgcgcaa gcttctgttt ctggggagct catgcattta
      241 tcccaagcac gcgccgcagc caattccaga gagcgcgcta ttgactggcc cgctcgaacc
      301 gaccaacgaa gcgtacgcgg tggcgaagat cgcgggcatc aaactttgcc aggcattttc
      361 gcgcgagtac ggcgacaact ttatctccgt gatgccgacg aatctttacg ggcctaatga
      421 caatttcgat ctcgaaacct cgcatgtgct ggcggcgtta ttgcgtaagg cacacgaagc
      481 taagcagaga aaagagcgcg agctggtcgt ctggggctcg ggcacaccgc gccgcgaatt
      541 tctgcatgtg gacgattgcg cagcggcctg tgtgttcctg ctcgagaatt acgattcgcc
      601 ggagatcatc aatgtcggct gcggggaaga cgtttcggtt cgcgagctcg ctgaattgat
      661 ttgcgatatt gtcggttttg agggcgaact gacgtgggac atgacgaaac cggacggaac
      721 accgcgcaag cttctcgatg tgacaaggct gcataaactc ggctggcgtc acaccatctc
      781 gcttcgcgac ggcatcgcgc gaacctatga gtggttcgtg aaaaacgcgg cgcgttaatt
      841 tgatgaagga attgcgcgtt ggcgtcgtcg gcgttggaca cattggcagc aaccatgcgc
      901 ggctttacgc cgaaattccc aatgcgcagt tcgccgcgat ctacgatgtc gatcttgcga
      961 aggcgaagac gattggaaaa aagtaccgcg cctgcgtcgc ggaatcgctt gatgagtttc
     1021 tcgaggcagt ggacgcagct tcgattgcta caccgaccag cacgcattac gaaatcgcgc
     1081 gggcgcttct cgcgcgcggc aaacacttgc tcatcgagaa gccgatcacc gacaacaccg
     1141 agcaggcgac cgaactttgc gagctggcat cgcgcagcgg tctgatcttg caggtcggtc
     1201 atgtcgagcg cttcaatccg gtgttgagtg cgctggagca acggttgact cacgcgcgtt
     1261 tcatcgaggc gcaccggctc tcgccttatc cggagcgtag tacggacatc ggcgtggtgc
     1321 ttgatctgat gattcacgat ctcgaagtca ttttgcatct ggtgcgttcg ccggtgcaga
     1381 acatcgacgc cgtgggtgtg ccggtgttga gccgcagtga ggacattgcc aacgcgcgct
     1441 tgcgctttaa gaacggttgc gtcgcgaata taacgtcgag tcgcatcagc ccggaacgga
     1501 tgcgcaaaat ccgggtcttt caggaagacg cgtacctttc gctcgattat gagacacaga
     1561 gtggcgaaat ctatcgacgc gctgacggcc acatcacacg cgataaagtt gagattgcgc
     1621 gcgaagaacc gctgaaaagc cagctcgtct ccttcgtcga atgcgcgaag accggacgac
     1681 agccgcgcgt ctccggattt caagcgacgg ccgcgctgga attggcagtc gaaattacga
     1741 agagaatcgc ggcgagtggg taagacgctt tacttcgtcg cgggtgaggc gagcgcggat
     1801 aatcacggcg ccgacctaat gaaggcggtg catgcgttga atgccgacgt cacgtttatc
     1861 ggccgcggtg gaccacacat gcgagctgtc gcgggccagg gtttggagaa ctggatcgcg
     1921 cacgccggcg tgctcggctt gtgggaagtg ttgaagcagt atggatattt ccgcgaaaag
     1981 tttcgcgaaa cgcttcggga agttgaaaag agcaggcccg acgccgtgat cctgatcgac
     2041 tatccgggct tcaacctgcg tctcgcccgg actctgcgca gacagcacgt ggcgcgcaaa
     2101 atcgtttact acatcagccc gcaagtctgg gcgtggaatc gtagacgcat cacgaagatg
     2161 gcgcgctgga tcgatctcat gctctgcatc ttcccgtttg aagccgagct ctacaacaag
     2221 tccggtttgc gcacggtctt tgtcggtcac ccgatgatcg atcggctccg ggcgcggaac
     2281 gtcgatattc gccgcgattc caacctgatc gggctgtttc ccggcagtcg cgcgcgcgaa
     2341 gtgcgaaaga tctttccgct attggtcgcg actgcgcggg agctgcgccg ggcgaatccg
     2401 actttgcgct tcgaaatttc agcggcctcg accgatttgg ccgcgcgaat ccaaagcgtg
     2461 ttagatcgac acccgcaaga tcgacaattc ttcgcgatta aagttgccga cgcgccgagc
     2521 acgatgcagc gggcacacgt gggcatcatc gcatcgggaa gcgcgacgct tgaggcagcc
     2581 tattttcgac tgccgttcgt gctcgtctac aaagtgacgt ggctgaccta tctcgccgcc
     2641 cgactgctcg tgcgcgtgaa gtttctcggc atgccaaatg tcctggcaaa caaagaaatc
     2701 gtgcctgagt tcattcagca cgaggcgcga gcgggcgcga tcgcgcgcgc ggttctgcgg
     2761 ctgatcaata acgaagttgc gcgcgaacgg atgatctctg agttcaacag aatcatcgcg
     2821 agtcttggcg agggcggtgc aagcgaacgc gcggcgcggg cgattctcgc cgaaatccgc
     2881 tgatgcgtaa tttctcagcg ggcgagctgt tccgaagtaa gcgcacattc ttcgcggtcg
     2941 ccgggctcac ggtgctgctt ttctgtgccg cgaatttgcc ctggcaactg gacgattacg
     3001 accaagcgaa gcaggccttt acctcatttg agatgatcaa agaaggacat tggctttacc
     3061 agcacacgcc gaatggccgc gtcgcgacga agccgccgct ggttggctgg acttcggcgg
     3121 cgctctttgt cgtgacacga tcatggggtg cggcctggcg attgccatcg ctgcttgccg
     3181 caatcgcgct ggcgacgctt ctgactcgtg tcgcaacttc tgcttacggg cgcgccgccg
     3241 gcttggtcgc cttcggggcg tttagctgga atctgtttac gccccggctc gccactctgg
     3301 tccgaaccga tatgccgctc gccctaacag tgtttctgat cggcacggtg atttgggaaa
     3361 agattcgcaa cgaggagccg tgcaagcgca gcgaacaact gtccatcttc gcattgttaa
     3421 cggcagccct gctgattaaa gggccgatcg tttacgcgtt tttgctcccg ggcgtggcgg
     3481 cgttcgcgta ctgtcgcggc agcgaccgcg tagccagcgc gtggtgcggt tggctacctt
     3541 ggaccgctcc gctcggcgta tttctggttt ggctcgttgg cggaattcgg tccgagtcac
     3601 gattttttga cgaggtcgtc aggcgtgaat ttctggcgcg tttcggcgaa acgatccatc
     3661 atccacagcc acttttgttt tacctgccgc atctgctcca taaatttttc ccttggagcg
     3721 cgttgatgct cgcactcggc gttgtcgctc ttggtttaca cagatggaac ctgcgatcgg
     3781 cattccgcca aacatcgccg tctacgctgt ggctggtttg ctggagtttg ggtggactga
     3841 ttttcatgtc gttcgttcct tcaaaacggg tcgaccgcat ttttccaatg gttccgccgc
     3901 tctgtttgtt gctcgcgtcg caacttgcca gggttgggtc tgaggacaaa gcgcgcgacc
     3961 gtgtctatcg gtggagcgct gcggcgctgt tattgtcgat cttgttcgcc ggtggctatt
     4021 ccgttgccaa ggtcgtgacc ggttatcacg aacaccgaga cgcactcgct gcgtttggac
     4081 gtgagattcg caatcaggcg gctgcaaatc actggcgtta cgaagtcatc agaggcagag
     4141 acgaagcaat tctgctttat ctcgagaaga cacattttgc cgaacccgcg aaggcgatca
     4201 gacaatggaa cgttgggcag ctggacgcgt tggttattcc ggacgatcaa ctgcctcagt
     4261 ttctcgcgcg gttgcgagat gtcggctcgc cgtcaatgca gtccggacgc cgcaaaaacg
     4321 tcgaccgccc gaattacatg ttactcacgc gcgactaatt ggcggctcga cttttcggcg
     4381 cctgatttta gtctcgcgac aattgcatgg cagacgagga gtttcccaag cgcgcgattg
     4441 aaacgctgtg ggcgccatgg cgagtcgatt atttcgagaa acaaccgcgc gaccgcaatt
     4501 tcctgagcgc cgcggcacga gcgagcgatg acgcggcgca tttggtcatc acgcggcgca
     4561 agaccgcatt cctgatgatg aaccgctatc cgtacgcggt cggccatttg atggcggtgc
     4621 cgtatcgcaa aacggccgac ctgtcgtcgt taggcgagaa cgaaatcatc gagctctggg
     4681 aattggtcgt gcatgcgcaa agacttctcc gcgtggccgc aaaagcgcag ggcttcaaca
     4741 tcgggctgaa catcggtgaa tgcgccggcg ccggtgtggt cgatcatctc catctccaca
     4801 tcgtgccgcg ttggagcggt gacacgaatt ttatgccgat gatttccggg acgcgcgcga
     4861 tttcggaagg actgtgcggt ctctatgaca agctcatcgc agcccaggcg gaggtcgaaa
     4921 actcaaggcg tcgaacatcg acatgacgac ttggatcgaa ccaccgcccc cgcaacgaaa
     4981 ggggctcggc tgctttgcca aaggctgcct gattctgctc gcgtttgtca tccttctggg
     5041 cgcggcgttt gccgccggca cgtatttcgc tgtgcgtttc ttgcgcggcc aatattttcc
     5101 ggcgcagcac attcaactgc cggcgagtac tgcaacgccc gaacaacaac aggtcgtgcg
     5161 cgcgcgttgg gatgcgtttg aagccacggc gcgcgctcgt cagccggctc gcatcgaatt
     5221 gacggcagac gatctcaacg cgctgatcgc atcggagccg cggcttcgcg gcaaggcgca
     5281 ggtgtccatt gaagaccatg tcggccattt gcgggtgagc gtgccgcttg atgccgtgcc
     5341 atggctacgc ggtcattacg tcaacgctga atgcacggtg caatcggctg cggatggaaa
     5401 tccggcggac gcccgcgtga gcagcattgt cgttaacggt cggccgatgg gcgaggaact
     5461 gttaggctgg cagtggcgct catggtcgct gcgccgttac atgtccgatt ggtcgaacga
     5521 gcgcaacctc gaccgcttcg agatcacggc cgggaaagtg attctggaaa ccaaaggcgg
     5581 ctaaaatcta cctggtttcc tgcggaggca gccgtatcgc aaagaagaat gcaggcggca
     5641 cgcctgccgc cacaatcacg ggcgatcgat caaaacgacc tcgccttgat atcgccagtc
     5701 atttgcatct cggaccagac cagcacggac aggattgtcg cgcacatagt tccacttttc
     5761 gccatagctg tcagcgctac gtaaaacgtg gtcgaagaaa ccgggttgcc aaagtttttg
     5821 cgttttgagc gcgaccgcga gcgcgcgttt cagcccgcca acccaggttg agaggacgaa
     5881 atccggacc
//
LOCUS       DASTDG010000194         8114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_155632, whole genome shotgun sequence.
ACCESSION   DASTDG010000194 DASTDG010000000
VERSION     DASTDG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155632"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="nadD"
                     /locus_tag="VFO30_06160"
     CDS             <1..592
                     /gene="nadD"
                     /locus_tag="VFO30_06160"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET9418907.1"
                     /translation="SSLSDLALKKIGIYGGTFDPIHHGHLILAREAVETLGLDKLIFV
                     PARVSPDKQRAPFASPETRLSMLQAAIAGESQFDANDCELRRAAPSYTIETIEQIRQR
                     EGDCELYCCVGEDNVPALPNWHRFDELKNMVRFVVLDRSGTESVHGYQVVQRKIDISA
                     TEIRKRVASRRSIRYLVPREVEEIIHRHNLYREALQ"
     gene            589..951
                     /gene="rsfS"
                     /locus_tag="VFO30_06165"
     CDS             589..951
                     /gene="rsfS"
                     /locus_tag="VFO30_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9418908.1"
                     /translation="MTAEALARACAELASNKKAEDIVVLDLRGISTFTDFFVICSATS
                     EPQLKAIAGEIETRLKERDGARPVAVDGYPASHWIVLDYLQAVVHIFHRDKREFYSLE
                     DLWGDAPRIEWAEPVAAR"
     gene            complement(987..1748)
                     /locus_tag="VFO30_06170"
     CDS             complement(987..1748)
                     /locus_tag="VFO30_06170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9418909.1"
                     /translation="MKHLIVFISLVLTAAPLFGQQKSPEQKSPEAKIDMDKVSYSIGL
                     QIGLNLQKQEEALKKQNVQINTDALVNGLKDALAGKPQLTTDQVRATLTAFEQDMTQK
                     QQAAGEKNKTDGERFLAENKKKDGVKTTPSGLQYKVIKEGNGPQPKETDTVVANYRGT
                     LIDGTEFDSSYKRGQTATFPLNRVIRGWTEGLQLMKVGSKYQFFIPANLGYGSQQMGP
                     DIGPNSTLIFDVELVGIQPPASAASPSPAASPSAK"
     gene            1980..2621
                     /locus_tag="VFO30_06175"
     CDS             1980..2621
                     /locus_tag="VFO30_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418910.1"
                     /translation="MTSWNAGEDFASLGIGHFIWYPEGKRGPFEESFPQLLAFMQDRG
                     VAVPHWLRSSCPWSTRSEFERAQSSPEMKELRDLLARAVDLQAEFLVYRLQHALPKML
                     EESAPANRANVQRQFERVGSSAGGCYALVDYVNFKGEGVLHTERYHGQGWGLLQVLEQ
                     MQGTSAGGSAAAEFSRAAAAILIRRVKNSPPARHESRWLPGWLKRVKSYATPG"
     gene            2618..3208
                     /locus_tag="VFO30_06180"
     CDS             2618..3208
                     /locus_tag="VFO30_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9418911.1"
                     /translation="MRRILAFALGFFFAWATSIHAQKSTATQMRKAAEMIAKQEPMKI
                     NQALLAQSKPDDTHLIISLPKQRAYLMLNDQLVIDSPISSGKRGHTSPTGHLRVLEKD
                     PNHRSNVYGDFVDSTGRTVRAGVSARIDAAPSGTHFIGAAMKWFMRLTEDGVGMHVGL
                     LPGYPASHGCIRMPSDAAKLFYDHVKVGTAVDVGDY"
     gene            complement(3202..4449)
                     /locus_tag="VFO30_06185"
     CDS             complement(3202..4449)
                     /locus_tag="VFO30_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9418912.1"
                     /translation="MRVALLNTGTEVLLGDVQEAHLRFIAREILPLGLRIAEQRAVPD
                     DAQAIRTALNDLFSRSDIMFITGGLGPTSDDLTCEVVAQSLGLKLERSAKLLAALEQR
                     FKMRGFKWTERVARQADVPAGAQVLANDNGSAPGFYLPANINPQIQSPHIFVLPGPSR
                     ELQPMFRDRAMSILCAIVADASPHERRLYKIAGISESAVEEAIGEQVFSIPGLELGYC
                     ARPGEVDVRIIGTPDAIRRADEIIRTELGKAIFTTVNETLEEVVVKLLAERKQTLAIA
                     ESCTGGLLANRITNVSGASEVFLAGYVTYANDAKIDILRVEPELLEKHGAVSEPVARM
                     MTEGARARARSTYAMSTTGIAGPSGGSAEKPAGTVYVGLASANQQTIVKKFFFPSDRE
                     TFKQLTTQAALDLLRRRLVSINQ"
     gene            complement(4560..5843)
                     /gene="clpX"
                     /locus_tag="VFO30_06190"
     CDS             complement(4560..5843)
                     /gene="clpX"
                     /locus_tag="VFO30_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9418913.1"
                     /translation="MARASNLTMCSFCGKSHAEVRKLIAGPGVYICDSCINVCKSILD
                     KELNEDARRQSSTIRVPKPAEIRRSLDEYVIGQEIAKKTLSVAVHNHFKRVLQTTTPP
                     ADPAAAFQPDPLADVEIEKSNILLIGPTGSGKTLLAKTLAKILDVPFCIADATTLTEA
                     GYVGEDVENIILRLLQNADYDVKRAEIGIVYIDEIDKIGRKTDNVSITRDVSGEGVQQ
                     ALLKILEGVTCNVPPQGGRKHPHQEYIQVNTEKILFICGGAFIGLDKIVQKRIGQKVM
                     GFGSPTLEVEDALRKEAIRHVEPEDLLAFGMIPEFIGRLPVVSSLDALTEEEMTAILT
                     ETKNAMVKQYTKLFAMEGVRLTVTKDALRALAGQAVTKGTGARALRSMLERIMLDIMY
                     DVPSREDIAEVTINRAVVEGKKLPLIRKKQDKDAA"
     gene            complement(5869..6573)
                     /locus_tag="VFO30_06195"
     CDS             complement(5869..6573)
                     /locus_tag="VFO30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9418914.1"
                     /translation="MSDSSENLSQSVLIPMVVEQTGRGERSYDIYSRLLKDRIVFIGT
                     PVDDHIANLVIAQLLFLQMEDGKKDINIYINCPGGVVTAGLAIYDTMQFLTCDVTTYC
                     LGMAASMGAVLLCAGTKGKRFALPNSDIMIHQVSGGAQGAASDVERQVDYMFKLKKRL
                     IKIIAQHTGKSEEQVRTDSDRDYYMSAAEAKEYGLVDEVIKSRKEVKLLDGATPPGSD
                     RSTAVAEAALPRKVGE"
     gene            complement(6570..7907)
                     /gene="tig"
                     /locus_tag="VFO30_06200"
     CDS             complement(6570..7907)
                     /gene="tig"
                     /locus_tag="VFO30_06200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9418915.1"
                     /translation="MKVEVEKQPNSVSTLRIELPPEEVSKEWDAIANSFARFAKIPGY
                     RPGKAPRAVIEKRFRKEIQEEVTKKLVSKSYHDAIAQKQLRVASVSNVEDVQFGEDKS
                     MRFRATVVTAPEFELPHYKDIPVSLPDTKVSAAEIDAAVERLREQAADFVDVTDRGLQ
                     MGDFTVLDFDGVVDGRPISEIAPNASKNLHGGRKFWVHVAPENFLPRFCEQLVGMERG
                     ETRSVQVEFPADFVVTELAGKKADYAVTLKEIKNRVLPPIDDALAAKLVPGKTLVDLR
                     DMIRHDLEHEKEHEVERAKELQIIKYLHEQTTFDLPPPLLQNETRRALNELVHRNRER
                     GVPDELLKGKEKELVEGAGAVAVHRLKTNFILQRIAEREKIQVTREDVDFRVRQDAAR
                     YNVSVDKMRKELGEHEGLDALAEQILLGKTLAFLKTNVSVQPAADETVAKDKS"
BASE COUNT         1616 a         2449 c         2188 g         1861 t
ORIGIN      
        1 gtcaagctta agcgacctcg ctttgaaaaa gattggcatc tatggcggaa cgtttgatcc
       61 gatccatcat ggccatctca ttctcgcccg cgaagccgta gaaacacttg ggttggacaa
      121 gttgatcttc gtgcccgcgc gcgtctcgcc ggataagcaa cgcgcaccgt ttgccagccc
      181 ggagacgcga ttgtcgatgt tgcaagcagc cattgcaggt gaatcgcagt ttgacgcgaa
      241 tgattgcgag ctgcggcggg cggcgccttc atacaccatc gaaacaatcg agcagatccg
      301 gcagcgcgaa ggcgattgtg agctttattg ttgcgttggc gaagacaacg tgccagcgct
      361 gccaaactgg catcgatttg atgaactaaa gaatatggtg cgctttgtcg ttctcgaccg
      421 gagcggaacc gaaagcgtcc atggctatca agttgtgcaa cgaaagatcg acatctcggc
      481 gaccgaaatc cgaaagcgag ttgcgtcccg gcgttcaatt cgctatctcg taccgcggga
      541 agtggaagag atcattcatc gccataatct ctaccgggag gcgttgcaat aaccgccgaa
      601 gctttagctc gagcctgcgc tgaactcgct tcgaacaaaa aagccgagga catcgtcgtg
      661 ctcgatttgc gcgggatttc caccttcacg gactttttcg tcatttgttc ggccacgtcc
      721 gagccgcaat tgaaagcgat tgccggtgaa atcgaaacgc gcctcaagga acgcgatggc
      781 gcgcggcccg tcgcggtcga cggttatcct gccagccact ggatcgtgct cgattacctg
      841 caagccgtcg ttcacatctt ccatcgcgat aaacgcgaat tttacagctt ggaagatctc
      901 tggggtgatg cgccgcgaat cgagtgggcc gagccagtgg cggcgcgcta aacctgcgcc
      961 ggcgcgctcg gcacaagctc ctacaactat ttcgccgacg gtgatgcggc aggactcggg
     1021 ctggccgcgc tcgccggtgg ctgaatgccg acgagttcga catcaaagat cagcgtcgaa
     1081 ttcggcccaa tgtccggccc catctgttgg ctgccgtagc cgaggttcgc cgggataaaa
     1141 aactgatact ttgagccgac cttcattaac tgcagtccct ccgtccagcc cctgatgacg
     1201 cgattgagcg gaaaggtggc tgtttggccg cgcttgtagg aactgtcgaa ctcggtgcca
     1261 tcaatcagcg tgccacggta gttcgccacc accgtatcgg tctctttcgg ttgcggcccg
     1321 ttgccttctt tgatcacctt gtattgcaag ccgctcggcg ttgttttcac gccgtctttc
     1381 tttttgtttt cggccagaaa tctttcgccg tcggtcttgt tcttttctcc ggctgcctgt
     1441 tgtttttgtg tcatatcctg ttcgaacgcc gtaagcgtgg cgcgcacctg atcagttgtg
     1501 agctgcggtt tgccggccag cgcgtccttc aatccattaa ccaacgcatc agtgttgatc
     1561 tggacgtttt gtttctttag cgcttcctct tgtttctgca ggttcaaccc gatctggagg
     1621 ccaatgctgt aactcacctt gtccatgtcg attttcgctt cgggtgattt ttgttccggt
     1681 gatttttgtt ggccgaacaa aggcgcagcc gtcaaaacta gagaaatgaa aacaatgagg
     1741 tgcttcatga ggcgggattg tgcgaatgaa ttgccgatgc ggctcgcgcc acggaaagct
     1801 gcacgctaat ggagaaagac agcacgtcaa ctcacggcag ggccagccgg cggctgttac
     1861 tcggcttgtg tgtagcactt gcctcgagtg ctgtcgcttt accatcgctg tcgcgagacg
     1921 atgcacttcg cattggaaag aaaatctggc aaaacgaatg caacggcacc gtcgcaggtt
     1981 tgacgtcgtg gaatgcgggc gaagatttcg cctcgcttgg tatcggccat ttcatctggt
     2041 acccggaagg aaaacgcggc ccgtttgagg aaagctttcc gcaactcctg gctttcatgc
     2101 aggatcgcgg tgtggcggtg ccgcactggc tgcgaagcag ctgtccgtgg tccacccgat
     2161 ccgaattcga gcgcgcgcaa agttcgccgg agatgaaaga actgcgtgat cttctcgcgc
     2221 gcgctgtcga tctacaggcc gagtttctcg tttaccggct ccagcacgcg ctgccgaaga
     2281 tgctggaaga aagcgcgccc gcgaaccgcg caaacgtgca gcggcaattc gagcgcgtcg
     2341 gcagttcagc cggaggctgc tatgcgctag ttgattatgt gaatttcaag ggcgaaggcg
     2401 tgctgcacac cgaacgctat cacggccaag gctggggttt gcttcaggtc ctcgaacaga
     2461 tgcagggcac aagcgctggc ggaagcgccg ccgcggagtt ctctcgcgcg gcggccgcta
     2521 tattaattcg gcgcgtcaag aattccccac ccgcgcgcca cgagtcacgt tggctccccg
     2581 gctggcttaa gcgcgtgaag agttacgcga caccgggatg aggcgaatcc tcgccttcgc
     2641 actcgggttc ttctttgcgt gggcgacgtc gattcacgcc caaaaatcca ccgcaacaca
     2701 aatgcgtaag gcggctgaaa tgattgcaaa gcaagagccg atgaaaatca atcaggcact
     2761 gctcgcgcaa agcaaacctg acgacaccca cctcatcatc tcgcttccca aacaacgcgc
     2821 ctacttgatg ttgaacgatc aactcgtgat cgacagccca atctcctccg ggaaacgggg
     2881 ccacacttca ccgaccgggc acttgcgcgt gctcgaaaag gacccgaacc atcgttcgaa
     2941 cgtttacggc gattttgtcg acagcaccgg gcgcaccgtg cgcgcgggag taagcgcccg
     3001 aatcgacgcg gcgccaagcg gaacgcattt catcggtgcg gcaatgaaat ggttcatgcg
     3061 cctgaccgag gatggcgtcg gcatgcacgt cggtcttttg cccggttatc cggcgtcgca
     3121 tggctgcatc cgaatgccgt ccgacgcggc aaaattattt tacgaccatg ttaaggtcgg
     3181 cacggcagtc gacgtgggcg actactgatt gatcgacact aatcttctgc gcaacaaatc
     3241 aagcgccgcc tgcgtggtca gttgcttgaa tgtctcgcga tcacttggaa aaaaaaactt
     3301 cttcacgatc gtttgttggt ttgccgaagc taaaccgacg taaaccgtcc ctgccggttt
     3361 ttcggccgag ccgccgctcg gccctgcaat tcccgtggtg gacattgcat aggtggatct
     3421 tgcacgcgcc cgtgcaccct ccgtcatcat gcgcgctact ggctcgctca ctgcgccgtg
     3481 cttctccagt aattccggct cgactcggag gatgtcgatc ttcgcatcat ttgcgtaagt
     3541 cacgtagccg gccaggaaaa cttcagatgc gccactcaca ttcgtgatcc ggttggcaag
     3601 caagccgccg gtgcacgatt ccgcaatggc gagtgtctgt tttcgttcag ccagcaactt
     3661 caccacaacc tcctccagag tttcgttaac tgtcgtgaag atcgctttgc cgagctcagt
     3721 tcgaatgatt tcgtcggcgc gtcgaatcgc atcaggtgtt ccgatgattc gcacatccac
     3781 ttccccgggc cgtgcgcaat agccgagttc caggccggga atcgagaaca cctgctcgcc
     3841 gatcgcttct tctacggccg actcacttat tcccgcgatc ttgtacaatc ggcgttcgtg
     3901 cggcgacgcg tccgcaacaa tcgcacacaa gatcgacatc gcgcgatcgc gaaacatcgg
     3961 ctgaagctcc cgcgacggtc cgggcaaaac gaaaatatgc ggcgattgaa tctgcggatt
     4021 gatgttcgcg ggcaaataaa atcccggtgc gctgccattg tcgttcgcga gtacttgcgc
     4081 gccggcgggc acgtccgcct gccgcgcgac acgttcagtc catttaaaac cgcgcatctt
     4141 gaaccgctgc tcaagcgccg cgagtagctt cgcgcttcgc tcaagtttca atccaagcga
     4201 ctgcgcgacg acttcgcagg tcagatcgtc gctggtcggc ccaagaccgc ccgtgatgaa
     4261 cataatgtcg ctgcgcgaaa acagatcatt cagcgctgtt cgaatcgcct gagcatcgtc
     4321 aggtactgca cgttgttcgg caattcgcag gcccagcggc aaaatctcgc gcgcgatgaa
     4381 cctcaggtgc gcctcctgca catcgccgag taaaacctcc gtcccggtgt tgaggagagc
     4441 gactctcatt gagcagcaaa cgtccaacgc tcaacgccca acgtccaatc ccgaactcgg
     4501 acgagccacc gagttcaaca ttcggcgttc gatgttcggc gttcggcctt tccgctgaat
     4561 tacgccgcgt ctttgtcctg cttcttgcgg atgagcggga gttttttgcc ttccaccacg
     4621 gcgcgattga ttgtcacctc ggcaatgtct tcgcgactgg gcacgtcgta catgatatcg
     4681 agcatgatcc gctcgagcat cgaacgtagc gcgcgggcgc cggtgccttt cgtcacggcc
     4741 tgaccggcca gcgcgcgtaa cgcgtccttg gtcacggtga gtcgcacccc ttccatcgcg
     4801 aacagcttcg tgtactgctt caccatcgcg ttcttcgtct cggtcaagat cgccgtcatc
     4861 tcctcttcgg tgagcgcgtc gagcgagctt acgaccggca atcgcccgat aaattccgga
     4921 atcattccga aggcgagcaa atcttccggt tcgacgtgac ggatcgcttc tttccgcagg
     4981 gcgtcttcga cttcgagcgt cgggctgcca aaacccatca ctttttggcc gatccgtttt
     5041 tgcacgatct tgtctaggcc gataaacgcg ccgccgcaga tgaacagaat cttctcagtg
     5101 ttgacctgaa tgtattcctg atgcggatgt ttccggccgc cctgcggcgg aacgttgcat
     5161 gtcacccctt cgagaatttt cagcaatgcc tgttgtacgc cttcgccgga gacatcgcga
     5221 gtgatcgaga cgttatcggt cttgcgacca atcttgtcga tctcgtcaat gtaaacaatg
     5281 ccgatctcgg cgcgtttgac gtcgtaatcc gcgttctgca gcaatcgcag gatgatattc
     5341 tcgacgtctt cgccaacata acccgcttcc gtcagcgttg tggcatcggc gatgcaaaat
     5401 ggaacatcga gaatttttgc cagtgtcttc gcgagcagtg ttttgccaga accggtcggc
     5461 ccgatgagca gaatattact cttctcgatt tcaacatcgg cgagcggatc gggctgaaat
     5521 gccgccgccg gatcggccgg cggtgtcgtg gtctggagaa cgcgcttgaa atgattgtgg
     5581 accgcgaccg agagcgtctt tttcgcgatc tcttgtccga tcacatactc atcgagcgag
     5641 cgccggattt ccgccggctt cggcacacga atggtagatg actgccgccg cgcatcttcg
     5701 ttcagttcct tgtcgagaat gcttttgcag acattgatgc aactgtcgca gatgtaaacg
     5761 cccggcccgg cgatgagctt gcgcacctcg gcgtggcttt tgccacagaa cgagcacatg
     5821 gtgaggttag aggcgcgcgc catattttgc ctctcgcagg agcagctttt attcccccac
     5881 tttgcgcggt agcgcagcct cggctacagc ggtggaacga tcgctgccgg gcggagtagc
     5941 gccgtccaga agtttaactt ccttgcgcga cttgattacc tcatcgacca acccgtactc
     6001 cttcgcttcg gccgccgaca tgtaataatc gcggtcggaa tcggtccgca cctgttcctc
     6061 cgatttgccc gtgtgctgcg caatgatttt gatcaggcgc ttctttaatt tgaacatgta
     6121 atcgacctgc cgctcaacgt cgctcgccgc gccctgcgcg ccgcccgaca cctgatggat
     6181 catgatgtcc gaattcggca gcgcgaaccg tttgcctttc gttcccgcac agagcaacac
     6241 cgcgcccatg ctcgccgcca tgcccagaca atacgtcgtc acatcgcacg ttagaaattg
     6301 catcgtatcg taaatcgcga ggccggctgt cacgacaccg ccggggcagt tgatgtagat
     6361 gttgatgtcc ttcttgccgt cctccatttg caggaagagc aattgcgcga tcaccaggtt
     6421 ggcgatgtga tcgtcaaccg gcgtgccgat gaaaacgatc cggtccttca gtaaacgcga
     6481 atagatgtcg taactgcgct cgccgcgccc ggtctgttcc accaccatcg gaatcagcac
     6541 cgactgcgat aaattctcgc tcgaatcgct catgatttgt ctttcgccac ggtctcgtcc
     6601 gcagccggct ggacgctaac attcgtcttt aggaaagcaa gagtcttacc gagcaggatc
     6661 tgctccgcga gcgcgtccag accctcgtgt tcccccagtt ccttgcgcat tttatcgact
     6721 gaaacattgt aacgcgccgc gtcctggcgc acgcgaaaat cgacgtcttc tcgcgtaacc
     6781 tggatttttt cccgctccgc gatccgctgc aggatgaaat tcgttttcaa tcggtgcacc
     6841 gcgaccgctc ctgcgccctc gaccagctct ttctcttttc ctttcagcag ttcgtccggt
     6901 acgccgcgct cacgatttcg atgcactagc tcgttcaacg cgcgtcgcgt ctcgttctgc
     6961 aaaagcggcg gcggcaaatc gaacgtggtc tgctcgtgca aatacttaat gatctgcaat
     7021 tcctttgcgc gctcgacctc gtgttctttc tcatgctcga ggtcgtgtcg aatcatgtcg
     7081 cgcagatcga caagcgtttt gccgggcacc aatttcgccg ccagcgcgtc gtcaatcggc
     7141 ggcagcacgc ggttttttat ttctttcaac gtcacggcgt agtccgcttt ctttccggcg
     7201 agctctgtca cgacaaaatc cgctggaaac tcgacctgca cacttcgcgt ttcgcctcgc
     7261 tccatgccga cgagttgttc gcaaaaccgc ggaaggaaat tctcgggcgc gacatggacc
     7321 cagaattttc tgccgccgtg caggttcttg ctcgcattcg gcgcgatctc tgaaatcgga
     7381 cggccatcga caactccatc aaagtcgagc acggtaaaat cgcccatctg cagtccgcgg
     7441 tccgtgacgt cgacaaaatc ggctgcttgc tcgcgcagac gctcaaccgc agcgtcgatc
     7501 tcggccgccg aaactttggt atccggcagg gacaccggaa tatccttgta atggggcagc
     7561 tcgaactccg gcgcggtcac aaccgtagcg cgaaatcgca tcgacttgtc ttcgccgaac
     7621 tgcacgtcct cgacattcga caccgacgcg acccgcaact gcttctgtgc gatcgcgtcg
     7681 tggtaacttt tcgagacgag cttcttcgtc acctcctcct ggatttcctt gcgaaatctc
     7741 ttctcgatca ccgcgcgtgg cgctttacca ggccgatacc cgggtatctt tgcgaaccgc
     7801 gcaaaactgt tcgcgatcgc atcccactcc ttcgagactt cttccggcgg caactcaatc
     7861 cgcagcgtcg acaccgagtt cggctgtttt tcaacctcga ctttcatgcg gaaagcgacg
     7921 ttaagccgcg cacatttgta gggcaaccgc tccggtggct gtcgtttcac ggcaggcaga
     7981 tgcgcctcct acccatcaag cgccaaacgc gccttcgcca ccggcacaag tcggctgcct
     8041 ccgcgcaaag gcgcgcgcaa cctgaacttg acggctttaa gccgtcaagt ctaacgagtc
     8101 cgtcacattg tagc
//
LOCUS       DASTDG010000195         8050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_116939, whole genome shotgun sequence.
ACCESSION   DASTDG010000195 DASTDG010000000
VERSION     DASTDG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116939"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFO30_06205"
     CDS             complement(<1..617)
                     /locus_tag="VFO30_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9418916.1"
                     /translation="MSFPINTRSTPSVALLFVLLLVLDLHAREPQKPPPATSTIGNKI
                     IRARDLGIPFDGTPGKLNAITDVAGVEVGYTTLISGQGKLVVGEGPVRTGVTAILPRG
                     HGSLNDPVYAGVFALNGNGEMTGTAWVEESGFLEGPVVITNTHSVGVARDATIGWRIQ
                     HGAADTTGYWWSLPVVAETWDGWLNDINGFHVRPEDVAHALDTAHG"
     gene            673..2232
                     /gene="dnaX"
                     /locus_tag="VFO30_06210"
     CDS             673..2232
                     /gene="dnaX"
                     /locus_tag="VFO30_06210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9418917.1"
                     /translation="MSYEVFARKYRPQTFDDLVGQEHVAQTLKNAVAQNRLAHAYLFV
                     GPRGIGKTSTARILAKSLNCIHGPTVTPCGVCDNCREIAAGNSLDVIEIDGASNNSVE
                     DVRELRDNVRYAPAKGRYKIYLIDEVHMLSSAAFNALLKTLEEPPPHVKFIFATTEPQ
                     RVLPTILSRCQRFDLHRIPANLIAQHLQFIATKEKITLEPAAAHAIARGAEGGLRDAE
                     SMLDQLVAFCGDPISEADVLKVFGFTSQQTVATLSDKILRSATADAVQLLQEQCEAGK
                     DMMRLMADLISHLRDLLVFKVKPEALSEDAGPELQSSLGTQAELIETDRLLELIDQFA
                     EAEGRMKWAPNKKLHFEVALIKAIQSLSQVTLNEVIENLNALRDGKANTGVGDPGYKS
                     DAARVAEKTTETSIDAAKLWQQLCAKIPARGFLRTLVDSLTVIGNDGRNFILGYPSED
                     KSSIETLASPSNRKQLEDMLHQISGRDWTLKLTPKEGLPPKKASAPVDDYKQDPLIRE
                     VLEVFKGQIKS"
     gene            2249..2548
                     /locus_tag="VFO30_06215"
     CDS             2249..2548
                     /locus_tag="VFO30_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET9418918.1"
                     /translation="MNLNKLMKQAQKMQEQMARTQAELEDRTIEVQAGGGKVKVTANG
                     AGEVLGIKIDKAIVDPNDVEFLEEVVLAGVQQAIEQGKALAQSEMAKIAGGLSEA"
     gene            2674..3855
                     /locus_tag="VFO30_06220"
     CDS             2674..3855
                     /locus_tag="VFO30_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9418919.1"
                     /translation="MIQRDYLILGSGVGGASACEAIRRYDKRGSVTLVGAEVFHPYKR
                     WILSKNFLREKTAPLKSLPLFEPAWYTSHKIETRFGTVVTQLNIDRRLAVLANGETIE
                     FNKACLAMGSRPVRPLVAGTSLGNVIYLRTVRDALALREMAGVEKTVMVVGGGLLACE
                     VAASLRQLKLKVGIMHRSGYLLNRYIDPNTGAWLTDYFAKHGITMLMGESLNGFEGKT
                     VLRNIQTKSGNRVPAGLAVLCYGAEPNLDLVRNTPLAGPHGSPVSDSLETEEKGIYAV
                     GDLAFYPDRLMGGMRRQTHWENAREQGLVAGANMTGKKRLRYEQIPYFWTEIFDLRLD
                     FVGDFSIQPTRIEMSGTQAKKKFIAQYYQADKLRGILLCQQSPREVDSAKTQLRRAVS
                     K"
     gene            complement(3918..4415)
                     /locus_tag="VFO30_06225"
     CDS             complement(3918..4415)
                     /locus_tag="VFO30_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418920.1"
                     /translation="MTLVLPSLRAGFYAGLGFALVLGMYLLWLWQPQRQVRRHTQHLF
                     EAVEKRNWSRVAEFIAPEFTDQWGDDHARLLERTREVLRYVRSIRITASDAWVLTQRC
                     RAVWTGKVTVEGEQGEITAEIKSRVNSLATPFELEWRRVSTKPWDWKLVRVSNRSLEI
                     PAEFE"
     gene            complement(4412..5368)
                     /locus_tag="VFO30_06230"
     CDS             complement(4412..5368)
                     /locus_tag="VFO30_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9418921.1"
                     /translation="MHRARSHSKARAQARARLATTSRPSLPRELRIGISGWRYAGWRG
                     KFYPKDLPQRRELEFAAKAFNSVEINGSFYSLQLPSSYRRWYEATPPDFLFSVKGARF
                     ITHMKTLRDIETPLANFFASGVLALGEKLGPILWQLPPNFGWNKERLEEFFMLLPRNT
                     HDAAKLGRKHDQKLKARAFLNIDVSRPLRYAIEVRHPSFLTPEFFALLRNHNIAFVFA
                     DSAGKWPYAEDLTADLVYIRLHGAEQLYVSGYSEAALDWWVNRIRYWCAGKQPHDAAL
                     IAPRRIDNRHSVSDRDVFVYFDNDAKVHAPFDAIRLAERLLS"
     gene            complement(5314..6069)
                     /locus_tag="VFO30_06235"
     CDS             complement(5314..6069)
                     /locus_tag="VFO30_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HET9418922.1"
                     /translation="MNSLRVGCVKYLNARPLIQGWHGHVEFDHPSALCRRLARGDIDL
                     ALVSSFEFLRNPIYRIVDCVSLSSDGPVYSVIVAHRGDISKVEQIELDPAAETSVNLL
                     RCLLAELSLTPRLVASSTSSRSGRLLIGDQAIEFRQQHGTEFHYWDLGAQWKGIVDLP
                     FVYALWLIRPEVGDANAIADELRALRDENLATLDELLAQEKKFDRAFCRRYYRDNLRF
                     SFGDREKRGLQRFAELCTELDLIPKRALKLELV"
     gene            complement(6133..7284)
                     /gene="mqnE"
                     /locus_tag="VFO30_06240"
     CDS             complement(6133..7284)
                     /gene="mqnE"
                     /locus_tag="VFO30_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HET9418923.1"
                     /translation="MNQLLIPRELKRIAEKVDTGQRISEADALTLYESDNLNAVAMIA
                     NAMRERKNGNHATYILNRYINYSNICVLSCQFCAFAAKKRDRHAFERAIEDIVGAVRE
                     ALEFGITEVHMVGGLHPTLKKDWYLRLLRELRALDPDLHIKAFTAIEVRHLAQRIFRT
                     SIRETLELLREAGLGSMTGGGAEIFDPVVRDEICRGKETAAQWLDVHRIWHKMGGRST
                     CTMLYGHIETLAHRVDHLRQLRELQDETGGFTGFVPFAFEPQTTVLAHIKPASAIEQL
                     RNLAVSRVYLDNIEHLTAYWVSMGLPLAQISLSYGVDDLHGTIMEEKIFHMAGATTPQ
                     QQTAAALEHAIREAGRIPVQRDSHYRHIAPAQKRSTAVARRTEAELACA"
     gene            7461..>8050
                     /locus_tag="VFO30_06245"
     CDS             7461..>8050
                     /locus_tag="VFO30_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9418924.1"
                     /translation="MDLERPGEVEDQDKEDVRLMELVGQGEMAAFEQLIERHQALVAG
                     TVARMLGSNSDVEDIAQQVFIRVWKSAARYTHRAKFTTWLLKITRNLVFNELRRIKRR
                     AQVPLQPDAETEEIGLNDQSSAAPDATLLEHELQQAIEEAIMQLPETQRMALVLRRYE
                     QLSYDEIAEVLELSVPAVKSLLFRARTELRERLSKY"
BASE COUNT         1804 a         2255 c         2425 g         1566 t
ORIGIN      
        1 ccgtgcgctg tgtcgagcgc atgcgcgacg tcttccggcc gcacgtgaaa gccattgatg
       61 tcgttcagcc agccgtccca tgtttccgcg accacgggca agctccacca gtagccggtc
      121 gtgtcggccg cgccgtgttg aatgcgccat ccgattgttg catcacgtgc gacgccgacg
      181 ctgtgcgtat tcgtgatcac aaccggtcct tctaagaagc cgctctcttc aacccaggcg
      241 gtgcccgtca tttcgccatt gccgttgagc gcgaaaacgc cagcgtaaac tggatcattg
      301 agcgacccgt gaccgcgggg aaggatcgcg gttaccccgg tgcgtacggg accctcaccg
      361 acgacgagct tgccttgacc gctgatcaag gtcgtgtagc cgacttcgac gcctgcgaca
      421 tcggtaatcg cgttgagctt gccgggcgta ccgtcgaacg ggatgccgag atctcgcgca
      481 cgaatgattt tgttgccgat ggtcgatgtt gcaggtggtg gtttctgcgg ctcgcgcgcg
      541 tgtaggtcga gcacgagcag gagcacgaac aggagcgcga cggaaggtgt cgatcttgta
      601 ttgataggaa aactcacggt gcggaatcgt tttctatttt cgggagcgaa gcaacgcatt
      661 tagatttgcc gtgtgagcta cgaagtcttc gcgcgcaaat accggccgca aaccttcgat
      721 gacctggtcg ggcaggagca tgtcgcgcag acactgaaga acgccgtcgc gcaaaatcgg
      781 cttgcgcatg catatctgtt cgtcgggccg cgcgggatcg ggaaaacttc gacggcgcga
      841 attttggcga aatcgctcaa ttgcattcac ggcccaacgg tgacgccgtg cggcgtgtgc
      901 gacaattgcc gcgaaatcgc agccggtaac agcctcgatg tcatcgagat tgacggcgcc
      961 agcaataaca gcgtcgaaga cgtgcgtgag ctgcgcgaca acgtgcgtta cgcgccggcc
     1021 aagggccgtt acaaaatcta ccttatcgac gaggtgcaca tgctctcctc ggcggccttt
     1081 aatgcgctgc ttaaaacact ggaggaaccg ccgccgcacg tgaaatttat tttcgccacg
     1141 acggagccgc aaagggtgct gcccacaatc ctcagccgct gtcagcggtt cgatctgcac
     1201 cgcattccgg ccaatttgat cgcgcaacat ctgcaattca tcgctaccaa ggagaagatc
     1261 acgctcgaac cggcggcggc gcacgccatc gcgcgcgggg ccgagggcgg actgcgcgat
     1321 gcggagtcga tgcttgatca actggtcgca ttctgcggcg acccgatcag cgaggccgac
     1381 gtgctcaagg tgttcgggtt caccagccag cagacggtcg caaccttatc agacaaaatc
     1441 ctgcgctcgg cgaccgccga cgcggtgcaa ttgctccagg agcaatgcga agctggcaaa
     1501 gacatgatgc ggctgatggc tgatctcatc tcccacctgc gcgacctact ggtgttcaaa
     1561 gtcaaaccgg aagcgctcag cgaagatgcc ggtcccgaat tgcagagctc gctcggtacg
     1621 caggcggagc tgatcgaaac cgatcggctg ctcgagttaa ttgatcaatt cgccgaagcc
     1681 gaaggacgga tgaaatgggc acccaacaaa aagctccact tcgaggtagc gctgatcaaa
     1741 gccattcaaa gcctcagtca ggttacgttg aacgaagtga tcgaaaactt gaatgcgttg
     1801 cgcgatggca aagcaaacac cggggtcggc gaccccggct acaaatccga cgcggcgcgg
     1861 gtcgctgaga aaaccacgga gacgagtatc gatgcggcaa aactgtggca acagctctgc
     1921 gcgaaaatcc cggcacgtgg atttttgcgg acgctcgttg attcattgac cgtgattggc
     1981 aatgacggcc gaaattttat cctgggttat ccgtcggaag acaaatcatc aattgaaacg
     2041 cttgcgagcc cgagcaatcg caaacagctg gaagacatgc tccaccaaat cagcgggcgc
     2101 gattggacat taaagctcac gcccaaagaa ggattgccgc cgaagaaagc atcggccccg
     2161 gttgatgatt acaaacagga cccgctcatc cgtgaagtgc tcgaggtgtt caaaggccaa
     2221 ataaaatcgt gatgaaagat cgacaatcat gaacctgaac aaactgatga aacaggcgca
     2281 aaagatgcaa gagcagatgg cgcgcaccca agctgagttg gaagatagaa ccatcgaagt
     2341 gcaagctggc ggcggcaaag tcaaagtcac tgccaacggc gccggtgaag tgctcggaat
     2401 caaaatcgac aaagcgattg tcgatccaaa tgacgtcgag tttctcgagg aagtagtgtt
     2461 agcaggcgtg caacaagcga tcgagcaagg caaggcgctc gctcaatcgg aaatggccaa
     2521 aattgccggc ggactgagtg aagcgtaacg ttggggcggc ggcggcggtt ggcacagcgc
     2581 ctctaacgcc aaaggcagac atcagagcaa gaccgagatc gatttttccc tcgccagcgg
     2641 gttagccggt ttctatcgtc ggctctttcg cgcatgatcc aacgcgatta tctcattctt
     2701 ggcagcgggg ttgggggggc gagcgcttgc gaggccatcc gccgctatga caagcgcggc
     2761 agcgtcaccc tggtgggagc ggaagttttt catccctaca aacgctggat actgtcgaag
     2821 aattttctgc gcgaaaagac tgcaccgcta aaaagcctgc cgctgttcga gcctgcctgg
     2881 tacacctcgc acaagatcga gacccgcttc ggcaccgtcg ttacgcagct taacatcgat
     2941 cggcggctcg ccgtcctagc aaatggcgaa acgatcgagt tcaacaaagc ttgcctcgcg
     3001 atgggcagtc gcccagtacg gccactggtc gccggcacaa gcctcggcaa tgtgatatac
     3061 ctgcgcaccg tacgtgatgc gctggcgttg cgcgaaatgg ccggcgtgga gaaaacggtc
     3121 atggtcgtgg gcggcgggct gctcgcgtgc gaagtggcgg caagcctgcg ccagctcaaa
     3181 ctcaaggtcg gtataatgca ccggagcggg tacctgctca accgttacat cgacccgaac
     3241 accggtgcgt ggttaaccga ttacttcgca aagcacggca tcacgatgtt gatgggtgaa
     3301 agcctgaatg gtttcgaagg caaaacggtc ttgcgcaaca tccaaacaaa gagcggcaac
     3361 cgcgtgccag ccgggttagc cgtgctctgc tatggtgcag agccgaatct cgatctggta
     3421 cgcaacacgc cgttggccgg gccacatgga tcgccggtat ccgattcgct cgagacagaa
     3481 gaaaaaggta tctatgccgt cggtgacctg gcattttacc ccgaccggct catggggggg
     3541 atgcgccgcc agacgcattg ggagaacgcg cgcgagcagg gcctggtggc cggcgcgaac
     3601 atgaccggga aaaagcgcct tcgttacgag cagattcctt atttctggac agagattttt
     3661 gatttgcggc tcgatttcgt cggcgatttc agcattcagc cgacgcggat cgagatgagc
     3721 gggacgcagg cgaagaaaaa gtttattgcg cagtactatc aagccgacaa actccgcgga
     3781 attttgcttt gtcagcagtc gccgcgtgaa gttgactcgg ccaagacgca actgcgccgg
     3841 gccgtttcaa aatagcgact ggagcaggtg cgccgtgggc gcgcagtgat cggacgcgat
     3901 ctcgtgtccc gatcgtgtta ctcaaactca gccggaattt cgagtgagcg attgctaacc
     3961 cggactagtt tccaatccca tggcttcgtc gatacgcggc gccattccag ctcgaacggc
     4021 gtcgcgagcg agttaacgcg cgactttatc tcggcggtaa tttcaccctg ctcgccttcc
     4081 acagttactt tgcccgtcca aacggcacga caacgctggg ttaggaccca cgcgtctgat
     4141 gcggtgatgc ggatggacct gacgtaacgc aacacttcac gcgtccgttc gagcagacgc
     4201 gcgtgatcgt caccccattg atccgtgaac tcgggagcga tgaactcggc cacacgcgac
     4261 cagttccttt tctccaccgc ttcgaagagg tgctgcgtgt gacgccgcac ctgacgttga
     4321 ggctgccaca gccagagcaa atacattccg agcactaacg caaaacccaa gccggcatag
     4381 aatccggcac gcaatgaagg aagtacgagc gtcacgagag aaggcgttct gccaaacgaa
     4441 tagcatcgaa cggggcgtga actttggcgt cgttgtcgaa atagacaaag acgtcgcggt
     4501 ccgagacgga atggcgattg tcgatcctac gtggcgcaat cagcgctgcg tcgtgcggtt
     4561 gtttgcctgc acaccaatag cggatccgat taacccacca atcaagcgcc gcctcactgt
     4621 aaccgctgac gtaaagctgt tccgcgccat gcagacgtat gtaaacgaga tcggccgtga
     4681 gatcttctgc atacggccat ttcccagcgc tgtcggcgaa gacaaaggcg atgttgtgat
     4741 tgcgcagcaa cgcgaaaaat tccggcgtga gaaaactcgg atgccggact tcgatcgcat
     4801 agcggagcgg ccgtgagacg tcgatgttaa gaaaggcgcg cgcttttagt ttctggtcat
     4861 gtttcctgcc gagcttcgcc gcatcgtggg tgttccgcgg caggagcata aagaactcct
     4921 cgaggcgttc tttgttccag ccgaaattcg gcggcaattg ccagaggatg ggcccaagct
     4981 tttcaccgag cgcgagtacg ccggaagcaa aaaagttggc cagcggtgtt tcaatgtcgc
     5041 gcagcgtttt catgtgcgtg atgaagcgcg cgcctttgac gctgaagagg aaatccggcg
     5101 gagttgcttc gtaccagcga cggtagctgg acggaagctg gagggaatag aatgagccgt
     5161 tgatctcgac ggaattgaac gccttggccg caaactcgag ctcgcgccgc tgcggcagat
     5221 ccttcggata aaattttccg cgccagccgg cgtaacgcca tccggagatg ccgatccgca
     5281 gctcacgtgg cagagacggt cggctggtgg tggctaaacg agctcgagct tgagcgcgcg
     5341 ctttggaatg agatcgagct cggtgcataa ctccgcgaat ctttgcagac cgcgcttctc
     5401 cctgtcccca aagctgaatc gcaggttatc gcgataatag cggcgacaaa acgcgcgatc
     5461 gaatttcttc tcctgtgcga gcaactcatc gagggtcgcg aggttctcat cacgaagcgc
     5521 gcgtaactca tcagcgattg cattcgcgtc gccgacttct ggccggatga gccagagggc
     5581 gtagacaaat ggaagatcga caatgccctt ccattgcgcg cccaaatccc aatagtgaaa
     5641 ttccgtgccg tgctgctgac ggaactcgat cgcttgatcg ccaatgagca gacgaccact
     5701 tcgtgaggag gtcgagcttg caacgagccg cggagtcaga ctcagctccg cgagcaagca
     5761 gcgcagcaag ttaacggacg tttccgcggc tgggtcgagc tcgatttgct ccaccttcga
     5821 aatgtctccg cgatgcgcca cgatcacgct gtacaccgga ccgtctgaag aaagagaaac
     5881 gcaatcgacg attcggtaga tcggattccg caaaaactca aaacttgaga caagtgcaag
     5941 atcgatatct ccgcgggcga gccgccggca caaggccgag ggatgatcga actcgacgtg
     6001 accgtgccag ccctgaatga gcgggcgcgc gttgagatat ttgacgcagc cgacgcgcag
     6061 cgagttcaag tgatcggcga tcagggatca gcacttcagt tcaccgacca ctgattactg
     6121 gtcactttga tttcaggcgc aggcgagctc cgcttcagtg cgccgagcta ccgcggtgga
     6181 tcttttctgg gcgggcgcga tatggcggta gtggctgtca cgctggaccg gtatgcggcc
     6241 ggcttcccgg atagcatgtt cgagtgcggc agcagtttgt tgttgtggcg ttgtcgcgcc
     6301 agccatatgg aagatttttt cttccataat ggtgccgtgc aggtcatcga cgccgtagct
     6361 cagcgagatc tgcgcgagcg gcaggcccat gctgacccag taagcagtga ggtgctcgat
     6421 attgtcgagg tatacgcggc tgacggcgag attgcgcagt tgctcaatgg ctgaggccgg
     6481 tttgatgtgc gcgagcacgg tcgtctgcgg ttcaaaggca aacgggacga agccggtgaa
     6541 gcctccggtt tcgtcttgga gctcccgcag ttgccgcaaa tgatcgacgc ggtgggcgag
     6601 tgtctcgatg tgcccgtaaa gcatcgtgca tgtgctgcgg ccgcccatct tgtgccaaat
     6661 gcgatggaca tcgagccact gggctgcggt ttccttgccg cggcaaatct cgtcccgcac
     6721 gaccggatcg aagatctcgg caccgccgcc ggtcatcgag ccgaggcccg cttcgcgcag
     6781 gagctccagt gtttcgcgga tggaggtccg aaaaatccgt tgtgcaagat ggcgaacttc
     6841 gatggcggtg aacgccttga tgtgcaagtc gggatcgagc gcgcgcaatt cgcgcagcag
     6901 gcgcagatac caatctttct ttaaggtcgg atgcagacca ccgaccatgt gcacttcggt
     6961 gatgccgaac tcgagcgctt cgcgcacggc gccgacgata tcttcgatag cgcgctcgaa
     7021 cgcatgccga tcgcgcttct tcgccgcgaa ggcgcagaac tggcacgaca agacgcaaat
     7081 gttcgagtag ttgatgtagc ggttgaggat gtaggtggcg tggttgccgt ttttccgctc
     7141 gcgcatcgcg ttggcgatca tcgcgacggc attcaaatta tccgattcgt aaagtgtaag
     7201 cgcgtctgct tcggagatgc gctggccggt gtccaccttc tcggcaatgc gcttgagctc
     7261 ccttggaata agcagctgat tcatccgggc gacgctatac tggcaaaagg atgcacacgg
     7321 ccattcccgg ttgcaaccaa acaatcactc gacacaaacg gcggcgtaaa ttcgcgaacg
     7381 tgacgttctt cagctaagat accggcgcac tttgtgactc gtcgcatctc tccgccacgt
     7441 ctgtcactgc agccgcgcgc atggatttag aacggccggg cgaagtggaa gaccaggaca
     7501 aggaggacgt gcgcctgatg gagctggttg ggcagggcga gatggccgcg ttcgaacaac
     7561 taatcgagcg ccaccaggcg ttggtcgcgg ggacggtggc acggatgctc ggcagtaact
     7621 ccgatgtcga agacatcgcg caacaggttt ttattcgggt ttggaaaagc gccgcccgct
     7681 acacacaccg cgcaaaattc accacctggc tgctgaagat tacacgcaac ctcgtcttca
     7741 acgagctgcg gcggattaaa cgccgcgccc aggtgccgct ccagccggac gccgaaaccg
     7801 aggagatagg gctcaacgac cagagcagtg cggcgccgga cgcgacgctg ttggagcacg
     7861 agctccagca ggcaattgag gaggcgatca tgcaactacc ggagacgcaa cgcatggccc
     7921 tggtgttgcg gcgctatgag caactgagct acgacgaaat cgcggaggtt ctcgagctga
     7981 gcgtgccggc cgtgaaaagc ctgctttttc gcgcgcgcac cgaattgcgc gagcgcctga
     8041 gcaaatattt
//
LOCUS       DASTDG010000196         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_922819, whole genome shotgun sequence.
ACCESSION   DASTDG010000196 DASTDG010000000
VERSION     DASTDG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922819"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..2658
                     /locus_tag="VFO30_06250"
     CDS             502..2658
                     /locus_tag="VFO30_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418925.1"
                     /translation="MIAGQKDIAAAGMRLQQTLDHELTTTRVALLESARAVLPRYLIF
                     GAEHVRELVSKLLAMENISIETLPRRKKSLRARERHLLLFLQRICAKNDTLSEFGPAS
                     WGKIVNAVASLSLAPGSGVSMRESFLERWTAHGIALAMNADPAVRPELSPRVHPNGRV
                     EDGHFVFTDAGERVRLNSDTLEILDRCDGRTQAHSLGFELGIFVRLADQKLIRWEVEV
                     PALEPHAFDVLVADVKRWRDGALRARWLNLLEPIAALPAKFIHTTDTVQRMEIMDQAR
                     QRLQTLGSERATSDRSLYSAINPIGEECFRDCGFEIAPELINEVATDAAPWIDLWRDN
                     YAFVASRIAAGLRGVLEKMSLKQNAAVPFPAFLRACEEANLSLTGPGLVTLGHIAFQE
                     VKAAFRSRMKPHAAKPEYELTPADCHFIRDSFKYEPFDEYTYPSADLQLAAESVEAVG
                     RGEYQWILAELHPPVALLHHGFYWCCPDKEQLSESLAATTFGRPHLHFGLYVADFTAT
                     TAVHLFDAPPGSCNFVAPQRGNPVWPTVRPADAEVYVDETSGDVCVRKLGSREYLGSL
                     ARGWIIPLGFHPFQFGMEPQMPRLRCGKVIVQRRAWVIRQDEFGAGNYTGVSRDLILA
                     VERLRAERDLPQFVYIRPTEQALRRSGAEGRDKDTKPVFVDLESYLFMEIFHRWLSKS
                     GELEVTEMLPAPHQLLWREPDGRRTFELRTLIIPSR"
     gene            2655..3479
                     /locus_tag="VFO30_06255"
     CDS             2655..3479
                     /locus_tag="VFO30_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9418926.1"
                     /translation="MKIVAIANQKGGVGKTTTAVNLGWALAEQGQRMLIVDLDPQANA
                     TSALGLAELQGESLYEALLGGASVADKIVPTRLERLFVIPADLDLAGAEVEIARMDNH
                     VTRLAETLSILRSDQTFDLIFLDCPPSLGILMTNALAAADEILTPIQCEYFALEGLVK
                     IVRVVEQVRDSGANDHIEIGGIVMTMYDSRTNLSGQVVAEVRQHFSERVYETVIPRTV
                     RLSEAPSFGKSVLEYDPRGAGADAYRRLAREFMQRHAAPATAPAQLQTPAWESLEK"
     gene            complement(3403..3933)
                     /locus_tag="VFO30_06260"
     CDS             complement(3403..3933)
                     /locus_tag="VFO30_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9418927.1"
                     /translation="MSMLETERLVLRPFREDDINGLAELFANPDFMRFSSGVYTREQT
                     AAFLDKIIGWERAGLPSLLALELKPERPLLGYCGFFHHPPEVTDDVEIGYRLHPHHWN
                     RGLITEAARAVRDHAFRDLKLARVISLVHPENTASRRVAEKNGMTVEKEIIFRGFPTL
                     VFAIEREQWLALHAAA"
     gene            4006..4647
                     /locus_tag="VFO30_06265"
     CDS             4006..4647
                     /locus_tag="VFO30_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET9418928.1"
                     /translation="MESRNKEKWQPAPVPETSSLSALRGAARSCTACHLYERATQTVF
                     GEGPKRAPIMLVGEQPGDYEDVAGKPFVGPAGKILDRALAEAGIKRDEVYVTNAVKHF
                     KWEPRGKRRIHQKPNSREIAACRPWMEAEMRLVKPKLVVCLGSTASQAFFGSSFRVTR
                     ERGKVLSSKLAPRVVATVHPSSLLRQPDEVSRDREYARFVVDLKAALKAAGQS"
     gene            complement(4741..5208)
                     /locus_tag="VFO30_06270"
     CDS             complement(4741..5208)
                     /locus_tag="VFO30_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418929.1"
                     /translation="MKNKISILSAIILAILFFCLSLTAQTTPRKSASPSVSPTVSASA
                     GGAAFSSAKATRPLPFHGMISAVDKDAKTFTITSKKTSRVFKVTDKTLITKGSAGATI
                     RDITANQEASGSYWKYSDGTLEAKNIKLGPMAKPKSPPPSPTLSPKFTTPPKQ"
     gene            complement(5520..5768)
                     /locus_tag="VFO30_06275"
     CDS             complement(5520..5768)
                     /locus_tag="VFO30_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9418930.1"
                     /translation="MPTFTYVYILQSKTHPERFYVGCTRDLRNRFVRHNTGEIAHTSK
                     WKPWRIKTYVAFSDNLRAKDFERYLKSASGRAFVKKHL"
     gene            complement(5886..5958)
                     /locus_tag="VFO30_06280"
     tRNA            complement(5886..5958)
                     /locus_tag="VFO30_06280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5923..5925),aa:Ala,seq:cgc)
BASE COUNT         1243 a         1774 c         1782 g         1294 t
ORIGIN      
        1 accacaacgg ctcgatcaaa gtcgagagca ctccgggcaa aggcaccacc ttcaccattc
       61 gtctgccgct tgcgcaatag ttgagacgcg ccgtcgcggc cactgtttct cagcgtgagg
      121 atcgaggtga cgggacattg gatgtagaac gttgggcgcc tgccgcgccg tagccttgcg
      181 aaggcgggtt gaacgttcgg tgtttgcttt ggcaagccac tttcactgct caactgctgc
      241 gcctttctct caccggcgcc gtgcgcttgc cgagccgtag cttagcgaag gctggtccgc
      301 ctctccctca gggagaggat tgaggtgagg gcagtcattg atcgctccac tttcacttgt
      361 cagcgttagc ggcttcgctt cgttggctgc gtgccttcgc cgtatcaagt cgctccggtt
      421 tttttaatcc gagttgccgg cgtacccttc gaacacgttg agcgactggc gactccggcc
      481 acgtgtgacg ctgcgcggga cttgatcgct gggcaaaaag acattgccgc ggccggaatg
      541 cggttgcagc aaacgcttga tcacgagctg accactacgc gcgtcgcgct tctcgaatca
      601 gcgcgcgcag ttctaccacg ctatctcatc ttcggggccg aacacgttcg tgaactcgtc
      661 tcgaaactct tggcgatgga gaatataagc attgagacgc tgccgcggcg caagaagagt
      721 ttgcgcgcgc gcgagcggca cttgctgttg tttctccagc gcatttgcgc caagaacgac
      781 actctgagcg aatttgggcc cgcgagttgg ggaaaaatcg tcaatgccgt tgcctccctt
      841 tccctagcgc cgggttccgg agtatcgatg cgagaatctt ttctcgaacg ttggaccgcg
      901 cacggaatcg ccttggccat gaacgcggac ccggccgtcc gccctgaact ttcgccacgc
      961 gttcatccaa atggtcgcgt tgaagacggt cactttgttt tcaccgacgc gggcgaacgg
     1021 gtgcggctca actctgacac acttgagatt cttgaccgtt gcgatggtcg tacccaagcg
     1081 cattcgttag gtttcgaact gggaatcttt gtccgattgg ccgaccaaaa actcattcgc
     1141 tgggaggtgg aagtcccggc gctggagcca cacgcgttcg atgttctcgt ggcggacgtg
     1201 aagcgctggc gggatggagc acttcgcgcc cgttggctca acctccttga gccgatcgcc
     1261 gctttgccgg cgaagttcat tcacacaaca gacaccgtgc agcgaatgga aatcatggat
     1321 caagcgcgac aacgtttgca aacgctcggt tctgagcgcg cgacctcgga tcggtcgctc
     1381 tactcggcca tcaatccgat cggggaagaa tgttttcgcg attgcgggtt cgagattgcc
     1441 ccagagttga tcaacgaagt cgcgactgac gccgcgccgt ggattgatct ctggcgtgac
     1501 aactacgcgt tcgtcgccag ccgcatcgcc gccggtttac gtggcgtgct cgagaaaatg
     1561 tcgcttaagc aaaacgcggc ggtgccgttc ccggcatttc tgcgcgcctg cgaagaagcg
     1621 aatctgtcat tgaccgggcc agggctagtc acactcggac atatcgcgtt tcaggaagtg
     1681 aaagctgcgt tccgctcccg aatgaaaccg cacgccgcca aaccagagta cgaactcacg
     1741 cctgccgatt gccatttcat ccgcgacagc ttcaaatacg aaccgttcga cgaatacaca
     1801 tatccatcgg cggacttgca gctggcggcg gaatcagtcg aggccgtcgg gcgcggcgaa
     1861 tatcaatgga ttctggccga actgcatccg cccgtcgcgc tgctgcatca tggattttac
     1921 tggtgctgtc cggataaaga gcaattgtcc gagtctctgg ctgcgacgac gttcggccgt
     1981 ccgcacctgc atttcggcct ctatgtcgcc gatttcaccg ccacgaccgc cgtgcacctg
     2041 ttcgacgccc ccccgggctc atgcaacttc gtcgcgccgc agcgtggcaa ccccgtctgg
     2101 ccaaccgttc gcccagccga cgctgaggtc tacgtcgatg aaacaagcgg cgacgtttgc
     2161 gttcggaaac tcggcagccg ggaatacctc ggctcactcg cacgcggctg gatcattccg
     2221 ctcggctttc atccgttcca attcgggatg gagccgcaga tgccgcgctt gcgctgcggg
     2281 aaagtgatcg tgcagcgccg cgcatgggtg atccgacagg atgaattcgg cgccggtaac
     2341 tacacgggcg tgtcacgcga tctgattctc gctgtcgagc gcttgcgcgc cgagcgcgac
     2401 ctgccgcagt tcgtttatat ccggccaacc gagcaagcac tccgccgcag cggcgccgaa
     2461 ggccgcgaca aagataccaa gcccgtattt gtggatcttg agagctactt gttcatggaa
     2521 attttccacc gctggctcag caaatcgggc gagctcgagg tcacggaaat gttgccggcc
     2581 ccgcatcagc tgctctggcg cgagccggat ggacgccgca catttgagct gcgcacgttg
     2641 atcatcccgt cgcgatgaaa attgtcgcga tcgccaatca aaagggcggg gtgggcaaga
     2701 cgacgacggc ggtgaacctg ggctgggcgc tcgccgagca gggacagcgc atgttgattg
     2761 tcgatcttga tccacaggca aacgcgacca gcgcccttgg tctcgcggaa ctgcagggcg
     2821 agagtctgta cgaagcgctg ctcggtggcg cgtccgtcgc cgacaaaatc gttccgacac
     2881 gcctcgaacg actcttcgtc atacctgccg acctcgacct cgccggcgcc gaagtcgaaa
     2941 tcgccagaat ggacaatcac gtgacccgtc tagccgagac attgtcgatc ttgcgctccg
     3001 accagacgtt cgacttgatc tttctcgatt gcccgccgtc ccttggcatt ttgatgacga
     3061 acgcgctcgc cgccgccgat gaaatcctga cgccgatcca gtgcgaatat ttcgcgctgg
     3121 aaggtttggt gaaaatcgtg cgcgtggtcg agcaggtgcg tgattccggc gcgaatgatc
     3181 acatcgagat cggcggaatc gttatgacaa tgtacgacag ccgcaccaac ttgagcggac
     3241 aggtcgtggc ggaagtccgc cagcacttca gtgagcgggt ttacgaaacg gtgatcccgc
     3301 gcaccgtgcg gctcagcgaa gcgccgagtt ttggaaaatc ggtgctcgaa tacgatcccc
     3361 gcggcgccgg cgcggacgcg tatcgccgac tcgcacgcga gttcatgcag cggcatgcag
     3421 cgccagccac tgctcccgct caattgcaaa caccagcgtg ggaaagcctc gaaaaatgat
     3481 ctccttctcc acggtcatgc catttttttc cgcaacgcgg cgcgaagcag tgttctccgg
     3541 atgcacgagc gaaatgacgc gcgccagttt taaatcgcgg aaagcatgat cgcgcacagc
     3601 gcgcgccgcc tctgttatga ggccgcggtt ccagtggtgc ggatgcaaac gatacccaat
     3661 ctcgacatcg tcggtgacct ccggcggatg atgaaagaaa ccgcagtaac caagcagcgg
     3721 gcgctcaggt ttgagctcca gcgcgagcaa cgaaggcaag ccggcgcgct cccagccgat
     3781 gattttgtcc aggaacgcgg ccgtttgctc gcgcgtatag acgcctgacg agaagcgcat
     3841 gaaatcagga tttgcgaaca gctccgccaa gccgttgatg tcatcctcac gaaacggacg
     3901 cagtactagg cgctcggttt ccaacatcga catctcgtcg cgcgcagcgt tttgccgttt
     3961 ttctggagac gaaccgtgcg cgaacttcgt atagtagaac gcaccatgga aagcaggaac
     4021 aaggaaaagt ggcaaccggc accggtgccg gagacatcga gcttgtccgc gctacgcgga
     4081 gccgcgcgga gctgcaccgc ctgtcatctt tacgagcgcg caacacagac cgtgttcggc
     4141 gaaggcccga agcgcgctcc gatcatgctg gtcggcgaac agccgggcga ttatgaagat
     4201 gtcgccggca aaccgtttgt cggtcccgca ggaaaaattc tggatcgcgc cttggccgaa
     4261 gccggtatta agcgcgacga agtttacgtc accaatgcgg tcaaacattt caaatgggag
     4321 ccgcgtggca aacgtcgtat ccaccaaaag ccgaactcgc gcgagatcgc ggcgtgccgc
     4381 ccgtggatgg aagcggagat gcggcttgtg aaaccgaagc tcgttgtatg tttgggatca
     4441 acggccagcc aggcgttttt cggctcatcg tttcgtgtca cgcgcgagcg cggtaaagtt
     4501 ttgtcctcaa aactcgcacc aagagtggtg gcgaccgtgc atccatcatc attgttacgt
     4561 cagcctgatg aagtgtcacg cgaccgcgag tacgcgcgtt ttgttgtcga tcttaaggcg
     4621 gcgctgaaag ccgctggcca atcgtaaaat tccggtactg ccgttgtcgc cacccgcctg
     4681 tggccgctcc gacgcgcacg aggaaacggt gcacagcgct acagtatcca tggattgaaa
     4741 ctactgtttt ggtggagtgg tgaacttagg cgacagggtc ggtgagggtg gcggcgattt
     4801 cggctttgcc atcggcccga gcttgatgtt tttcgcctcg agcgtgccgt cgctgtattt
     4861 ccaatatgag ccacttgctt cctgattcgc ggtgatgtct ctgatcgtcg cgccagctga
     4921 ccctttggtg attaacgttt tgtccgttac cttgaagacg cgggatgttt ttttgctcgt
     4981 aatggtgaac gtcttcgcgt ccttatcgac cgccgaaatc atcccgtgaa acggcagcgg
     5041 ccgggtggct ttcgccgatg agaatgccgc accccccgcc gaagcactga ctgtcggcga
     5101 aaccgaaggt gaagcggatt tccgcggagt cgtctgtgcg gtcagtgaca aacaaaagaa
     5161 cagaatcgcg agaataatcg cacttaagat cgagattttg tttttcatgg cgcgattcgg
     5221 ttcgcttgag gacgtgacca gtcccgctgg atgggacgaa acgatcgccg cttctgttca
     5281 actacggcgc gacgggctac tctctgctac cgctgcgagc gaagactggt acacgtatgt
     5341 aaacgtaggc acgaactggc atgccgagtc gtagctcgct gcgttcatat ggcgagccta
     5401 aagatgtccg ccttcgccca ggcaacggcg cgacagtctg cgctctccgc taccgctgcg
     5461 agcgaagact ggaggtaagg ggattacgct cccgctggcg gatccaataa tatgaaacat
     5521 cagagatgct tttttacgaa tgcgcgtccg gatgccgatt tcaaataacg ctcaaagtct
     5581 ttagctcgca gattgtcgga aaaagcgaca taggttttga ttcgccatgg tttccatttg
     5641 gaggtgtgcg ctatctcacc tgtgttatgg cgtacaaatc ggttgcggag atcacgcgtg
     5701 cagccgacgt aaaagcgctc gggatgtgtc ttgctttgca gaatgtagac gtatgtaaac
     5761 gttggcacga actggcatgc cgagtcgtag ctcgctgcgt taatatggcg agcctaaaga
     5821 tgtccgcctt cgcccaggct acggcgcgac agtctgcgat ctccgctacc gcggcgagcg
     5881 aagactggag gtaaggggat tcgaacccct ggccttctca ttgcgaacga gacgctctac
     5941 cagctgagct atacccccga aatctttgaa aagccgacgt ttaacgtgca gaatttggtc
     6001 tgcatttcaa ctgaagcccc acccgttttg cccccctgat gattttcgga gagctcactt
     6061 ctgtccaagc aacgatgaca tggattttgc ttc
//
LOCUS       DASTDG010000197         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_273533, whole genome shotgun sequence.
ACCESSION   DASTDG010000197 DASTDG010000000
VERSION     DASTDG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273533"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VFO30_06285"
     CDS             complement(<1..1093)
                     /locus_tag="VFO30_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9418931.1"
                     /translation="MNSPVSQSVVDLLLASGVSSREEASQLAQNLNGGSWVAQVLDSG
                     KVDETRFLTAIGEFFKVPVQSIDSKKINRETLSILPSRFVFQHHILPIEVKDNLVVLA
                     TYDLFNTIGRQLVSQLLKKPVEWVLVPRGQLLRAIKGLYGVGAETFDEILKTNRTFEI
                     LQDGETATDLSADDPEASVVKFVNQIIREAIAERATDIHVEPLENDLRIRYRIDGILH
                     EVAVPPQLRLLQSAIISRLKVMAHMDIAERRLPQDGRMNLHANNQEIDVRVSTIPTVN
                     GESISLRLLSRTDQQFGFERLDLSDKQEKIIRHLLAQPNGIILLTGPTGCGKSTSLYC
                     FLSTINSVQRRIITIEEPVEYRLPGVSQID"
     gene            complement(1129..1572)
                     /locus_tag="VFO30_06290"
     CDS             complement(1129..1572)
                     /locus_tag="VFO30_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET9418932.1"
                     /translation="MKPSSETKLRRFSAILFILAAIIALAGLADATYLTVQALTGETL
                     VCGGSADCSRVLASPYARVAGVPVAAFGCLAYFAAFAFATFAGFGYRRARTLLSITVA
                     AMFLVTLWLLYVQAFLLHAFCRYCLFSAALVFLLAALVVLTPRSN"
     gene            complement(1685..>2048)
                     /locus_tag="VFO30_06295"
     CDS             complement(1685..>2048)
                     /locus_tag="VFO30_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418933.1"
                     /translation="LLAFTATSACMMEAQLQGGSESGVEGTITVSPASPGPARLGVPN
                     SRPLANTTFLVQGAASPVRTFTTDAHGQFCVLLPPGHYTVSLKDSQPRIGRFGAFEVD
                     VISSKMTSVSWQCDSGMR"
BASE COUNT          469 a          575 c          612 g          392 t
ORIGIN      
        1 cgtcgatctg cgaaacaccg ggcaagcgat attcgaccgg ctcctcaatc gtgatgatcc
       61 gccgctggac cgaattaatc gtcgagagaa aacagtaaag cgacgtcgat tttccgcagc
      121 cggtcgggcc ggtgaggaga atgatcccgt tcggttgcgc gagcaggtga cgaatgattt
      181 tctcctgctt gtcgctcagg tcgagccgct caaaaccaaa ttgctggtcg gtgcggctga
      241 gcaaacgcaa gcttatcgat tcgccgttta ccgttggaat cgtcgagacg cggacgtcga
      301 tttcctggtt gttagcgtgc agattcatgc gcccatcctg gggcagacgc cgctcggcga
      361 tgtccatgtg cgccatgacc ttgagccgcg aaataatggc cgattgcaac aggcgcaact
      421 gcggcggcac ggccacttca tgcaaaatgc cgtcaatgcg atagcgaata cggaggtcat
      481 tctcgagcgg ctccacgtgg atgtcggtgg cgcgctcggc gatggcttcg cggatgatct
      541 gattgacgaa tttgacgacc gacgcttcgg gatcgtccgc gctcaaatcc gtcgcggttt
      601 cgccgtcctg caaaatttcg aacgtgcgat tcgtctttaa gatttcgtcg aacgtctcgg
      661 caccaacccc ataaagacct ttgatggccc gcaaaagctg cccgcgcggg accagcaccc
      721 attcgaccgg tttcttcagc aattgcgaga cgagctgccg cccgatcgtg ttgaaaagat
      781 cataggtcgc gagcacgacc aagttgtcct tcacctcgat gggcaggatg tggtgttgga
      841 aaacgaaccg gctcggcaag atcgacaacg tctcccggtt gatctttttc gaatcaatcg
      901 attgcaccgg cactttgaag aactcgccga tggcggtgag aaacctggtc tcgtccactt
      961 tgcccgaatc gagcacctgt gcgacccacg agccgccgtt gagattttgc gcgagctgtg
     1021 acgcttcctc gcgggaggag acaccgctgg cgagtaaaag atcgacaact gattggctga
     1081 cgggcgagtt catttcaaag ttgcgtactt aatatagtag gcgggttttc aattcgagcg
     1141 aggagttaag acgaccaatg ccgcaaggag aaagacgagc gcggccgaga acaggcagta
     1201 gcggcaaaac gcgtgcaaca ggaacgcctg cacgtagagc agccaaaggg tgaccaaaaa
     1261 catcgcggcc acggtaatgc tgagcaatgt gcgtgcgcgc cggtagccga aacccgcaaa
     1321 ggtggcgaaa gcgaatgcag cgaaatacgc cagacaacca aacgccgcca ccggcactcc
     1381 ggcaacacgc gcgtacgggc tggcgagcac acgcgagcaa tccgccgagc cgccgcaaac
     1441 gagcgtctcg ccggtcagcg cctgcaccgt caggtacgtc gcgtcggcga ggccagccaa
     1501 ggcgataatg gcagcgagaa tgaaaaggat ggccgaaaac cgccgtagct tcgtttcgct
     1561 gctgggcttc atcgattcga cacgaacggc gtgcaaggtt cgacggaaga agtgggtcgt
     1621 caactaatgg ctgcgtgtgg cgagcggcta cgatgcagct gccgaaacga cgactcggtc
     1681 gtattcagcg catcccgcta tcgcattgcc acgacacact tgtcattttg ctggatatga
     1741 catctacctc gaatgctccg aagcggccaa tgcggggttg gctgtccttc agcgaaacgg
     1801 tgtagtggcc gggcggcagc agaacacaga attggccgtg cgcatcggtg gtgaaagtcc
     1861 tgacggggct ggctgcgcct tgaacaagaa acgtggtatt cgcaagtgga cgcgaatttg
     1921 gcacacccaa gcgcgccggc ccaggagatg ccgggcttac agtgatcgtt ccttcaacgc
     1981 ccgactccga cccgccttgt aattgcgctt ccatcataca agccgatgtc gcggtaaatg
     2041 cgagcaag
//
LOCUS       DASTDG010000198        13823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_692596, whole genome shotgun sequence.
ACCESSION   DASTDG010000198 DASTDG010000000
VERSION     DASTDG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692596"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFO30_06300"
     CDS             complement(<1..339)
                     /locus_tag="VFO30_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418934.1"
                     /translation="MPVPAVHVNLDDAWLDEPLGLPALDAQAWGPRLRFSAPRRLVEE
                     FYAEIKDQVAPFLIYGSGDFHYLTALWLRRVRPRVVLVSFDNHPDWDIRPPKWCCGGW
                     VNRALELPHVE"
     gene            400..873
                     /locus_tag="VFO30_06305"
     CDS             400..873
                     /locus_tag="VFO30_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9418935.1"
                     /translation="MEKTEKQELKEHEIERDFFCVIRDAFRVFAQRASILVGTPWVFA
                     LAILVIVIWGITGPKFHYSDTWQLIINTGTTIVTFLMVFVIQNTQNRDAKAIHLKLDE
                     LIRAIKGARNKLVDLEELSDDELKKLEQEFQRLRKKSAETDTRVAKVETQQRNSR"
     gene            complement(870..1544)
                     /gene="nth"
                     /locus_tag="VFO30_06310"
     CDS             complement(870..1544)
                     /gene="nth"
                     /locus_tag="VFO30_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9418936.1"
                     /translation="MTAHDRVTELIEVWPQVYPGAHCELDYTNPLELLIATILSAQCT
                     DKRVNMVTPALFKKYRTANDYGSAPPAALEKMIKPTGFFRNKTKSIRAATSTIIDKFG
                     GKVPNSMEKLRQLPGVGRKTANVVLGNAFGKNEGVVVDTHVARLSQRLGLTKQTDPDK
                     IERDLMKLVPREHWTNWSHWLIWHGRRRCFARKPDCRQCEIFRLCPSGKVFVRTGQAR
                     AEANPT"
     gene            complement(1541..1807)
                     /locus_tag="VFO30_06315"
     CDS             complement(1541..1807)
                     /locus_tag="VFO30_06315"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET9418937.1"
                     /translation="MQRIREFVARLRLQQAPQLLRKIVVAVIGTTVLLIGLALVVLPG
                     PAVIVIPIGFAILASEFIWARRVIKRGRILVGRVRRVVKGTPAR"
     gene            complement(1858..3126)
                     /gene="hflX"
                     /locus_tag="VFO30_06320"
     CDS             complement(1858..3126)
                     /gene="hflX"
                     /locus_tag="VFO30_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9418938.1"
                     /translation="MIETSPQRKRERALLIGLEKEGVSKWDLRDSMEELAELANSAGA
                     EVVDTVTQKLQKPTAPYYIGRGKAESLKHALQDRQVTSVIFDDELSPAQGRNLENLLA
                     RKVLDRTQLILDIFAQRARSREGRLQIELAQLQYLLPRLTGMWHHLSRQTGGIGTRGP
                     GETQLEVDRRRVQERIARLERELEAVRKTRAVQRQGRKRHQWPVAAVVGYTNAGKSTL
                     LNLLTGADVTTEDKLFATLDPTTRSFVLPNKQRVLLTDTVGFLRKLPHTLIESFKATL
                     EEVSEADLLIHIVDLSHPRAEEQMEAVDNVIKQLDAYGKQTLIVFNKIDNLPDRELAD
                     TYVKRFSCSVAISARTGEGVNKLVQALQDALSAWRLRSRFRIPAKESALLAEIHRVGH
                     VLELKYEGDNAVLVAHVPPHLEAKLEQYSA"
     gene            complement(3119..3844)
                     /locus_tag="VFO30_06325"
     CDS             complement(3119..3844)
                     /locus_tag="VFO30_06325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9418939.1"
                     /translation="MRGVFFIVGPTASGKSELAADVARELGVEIVNADAFQIYEGFEV
                     ITAKPDAATLAKARHHLIGMVSILEEMNAEKFRRAALEAIAEIHSRGKTALVVGGSGL
                     YIKALTHGLHRDWKKVDVHPAGVFVLRGRAELYDRINRRVEAMFNRGIIEEVRAVGAM
                     SETASKMIGVREIRQLLAGKMSMLQCIAAIQQATRRYAKRQLTWFRRQTNFPPLNLSG
                     LTHKEAVHWISLRAQRSHARRDD"
     gene            complement(3948..4448)
                     /locus_tag="VFO30_06330"
     CDS             complement(3948..4448)
                     /locus_tag="VFO30_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9418940.1"
                     /translation="MNSHSSGGRSRRSRGGSRHRGDRRRRQDSGPAKAPKKTFWQRLA
                     AFFANGKTARAPAMAARNGAPASRPQRKPEPVEVTSPRLYVGNLSFDATESDLFELFN
                     GVGHVQNAEIVSYRHNQRSKGFAFVHMQTIEEAKRAVEELHDKEFLGRKLVVSGAKST
                     ERQSHS"
     gene            complement(4764..5774)
                     /locus_tag="VFO30_06335"
     CDS             complement(4764..5774)
                     /locus_tag="VFO30_06335"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9418941.1"
                     /translation="MTSFLPFDQRACFIAGPAKSGTTLLVALLDSHPELLVLPEETAY
                     FPTVLTKYAPRGRRAQFDYITKQSLSNVLFGGKCKWGKRDYSYFPTAPFLETFERMAF
                     DPVNADKDLLVILMEAYAMTLGRPLDSIRRWIEKTPANRRYLPAIRQHFPHAKVLVTI
                     RDPRAILAAQIALEKTRRLGTFSTYYCISHWREAATTALRAMRGYDPDALAVPYEKLV
                     LDTAGVMKRVCAFLEVAYDPAIVLAPTKAGKFWAGNSAAETSFVDINAEPLTRWESDL
                     SEEEIGWVEWHCRDMMPTFDYEPKLSRRALRYFFKPVRLERPKQYLKSRFYSLRDDWV
                     RR"
     gene            complement(5881..6828)
                     /locus_tag="VFO30_06340"
     CDS             complement(5881..6828)
                     /locus_tag="VFO30_06340"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9418942.1"
                     /translation="MRVLVTGGAGFIGSHLTEKLLAAGHEVAILDDFNDFYDPAIKRT
                     NIAGIVRDVRVHHVDLRDGAAVRNVLGSENFDAIAHLAARAGVRPSIAQPQLYYDTNV
                     VGTLRLLEAARASGIKRFIFASSSSVYGISKTVPFSEDQHLTQTISPYAATKIAGEFL
                     CSTFSHLYNIRVVALRYFTVYGPRQRPDLAIHQFTRKIYAGKPIDQFGDGTTRRDYTY
                     IDDIIQGTMAALEYDGALFDIFNLGESETIQLKDLIAAIENALGKKAKINRLPEQPGD
                     VPLTCADISKAKALLGYNPTTPLKDGLPRFIDWFLRNEK"
     gene            6931..7590
                     /locus_tag="VFO30_06345"
     CDS             6931..7590
                     /locus_tag="VFO30_06345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9418943.1"
                     /translation="MRKLFGLAVGAFALGFVAIVGLQLWPELTRSKYVDLAFRIPSPF
                     ELASLPIAARFDFPLGSENGALAYNAQRFTENRHLGDDLNGIGGENSDLGDPIYCVAD
                     GRVLLARDGGTGWGNVVIVLHAYLENGGRNYVQSYYAHVEKMLVHPGDDVRRGQQIAT
                     VGTGGGRYFAHLHFEMREFVTPFIGPGYREDTRGWLDPSAFIEAHRGAPEEDVGRAPL
                     E"
     gene            complement(7597..8091)
                     /locus_tag="VFO30_06350"
     CDS             complement(7597..8091)
                     /locus_tag="VFO30_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET9418944.1"
                     /translation="MLRWRAAVGDPDPDRDSFRKSIGRLMQFHRRQLAPNELDHELIW
                     LSVSLASFACAGAWLGFGLPWPQCVFHHVTGLPCITCGMTRSAIAFFHGDLLRAFNWN
                     PLIFVSLCGLSIFDFYAFAVVITRAPRLRISFSAAAQNYGRGTMVSALALNWMYLLAH
                     WQKF"
     gene            complement(8025..8903)
                     /locus_tag="VFO30_06355"
     CDS             complement(8025..8903)
                     /locus_tag="VFO30_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9418945.1"
                     /translation="MIHVSRGATTFGAFSEEDVREGLRTSRFMPSDLGWREGMQNWQP
                     LSQFEEFAAETAGAAPPPPSIAPSTVPPSVSPAASEVPVSRTGLPWDERDQKGWFNAF
                     IETLQTVLTRPDYAFRIMKVEGGLAGPIIYALIGGCAGGIVSFLFSLALQSIGLTVGR
                     HNTFAWLTGIGAGSVVFIILLPIVIVLGLFIGGAIVHLCLMIVGGANKTFETTLRVLA
                     FTQGSTGPLQMIPICGGIIAGVWALVLNCIGLARAHETETGRAVLAIFLPLMVCCGGV
                     LLLAILIPTAIHFGNR"
     gene            complement(8965..10134)
                     /locus_tag="VFO30_06360"
     CDS             complement(8965..10134)
                     /locus_tag="VFO30_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET9418946.1"
                     /translation="MHDLFSEEDVDLAATNSAAPLATRMRPRSLDEFVGQEHILAPGK
                     LLRRAIEADRLPSVILSGPPGTGKTTLAHIIADMTHAQFVRLSGVESNVAEMRRVVAG
                     AANRLRNAGQKTILFIDEIHHFNKAQQDILLPDVESGNLRLIGATTYNPFFYVNSPLV
                     SRSQVFQLEPLRVNQLELLIDRALADKERGLANYNVKMDGDARRHLAKLSDGDARKCL
                     NALEIGVLTTPADGNSVVHFTVAVAEESIQQKAIVYDRAGDAHYDTISAFIKSMRASD
                     EPGAMYWLAKMLHAGEDFRFIARRIVIFASEDVGLADPEALPLAIATQQAVEFVGLPE
                     ARIPLAHATAYMCRANKSREAYDALNAATEKIESEQTQRVPEHLKNIHFPVNPDE"
     gene            10232..12565
                     /locus_tag="VFO30_06365"
     CDS             10232..12565
                     /locus_tag="VFO30_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9418947.1"
                     /translation="MPLIGSDVTSRPSRHWRARQPFVGLVIAAVAGVLLADYLAIPCT
                     AWLPMTGIFALAAIATYCWPHATSTRALVGFGFFLLHTFRTHDTPGQRLVERLGDRAR
                     VITAIGTVTSEPKVAPNGFATFLVTLESVQLEDTTVRSRATCLVRWRGTPEFGNELKV
                     FGIAEPIPPPRNPGEFDMRGYLARRDVRRLLFVRYPEDGVLLRHGAGNPILRLAQKSR
                     AWMQLALCRGLENAPDVQAFLSGIVLGQRHQTPEDIEEPFQQTGTLHIFAVAGLHVGI
                     VARLLWMLGVVAQLPRKLATALIIPLLLFYAAITGLHVSSVRAAVMSSVLLGGFFVER
                     KVFSLNSLAAAAFLILCWDTNELFSTGFQLSFAVVGAIILFSHLPFAYLWPLAAPDAF
                     LPGALIRGARRWLHTGLEWLCRAASVSFAAWAGSLLLLLWYFYLVTPISLVANLIVVP
                     MAFFILAIALLSMVAAPFLPSFSILFNNANWCLARLVLGIVHLLAQLPGGHYYIAHPR
                     WPANATATITVLDVGTGAAIHVRSRGQDWLFDCASERDYQRVVRPYLHASGVNGIDGV
                     LLTHGDSLHIGGALGLLEDFARPQLIDNPAPDRSIIHRRLRTIFQQRNLSVRNMVAGE
                     TFTIANEVTARVVHPPSGFAESNADDEASVVQVAVSPMTKILLTSDAGLPTENALLAS
                     GIDLKSDILIKGEHRSATSGSTRFLDAVRPQLIIATSRDFPESERISDDWAARMRARG
                     IKLFRQDETGAVELQFGRREWSARAHVTGEVFRSANR"
     gene            complement(12526..13107)
                     /gene="trmB"
                     /locus_tag="VFO30_06370"
     CDS             complement(12526..13107)
                     /gene="trmB"
                     /locus_tag="VFO30_06370"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET9418948.1"
                     /translation="MIELIPNSYVARLNLEKIFGRVALLHVDLGCGDGSFLCALAELH
                     PDENFLGIERMSGRVDKACRRARALENVRVLHLDTSYAVRYLFPESSVAVFYLLFPDP
                     WPKRRHYRRRVVTSDFLDAIRAALVDEGLLKVATDQWDYFRQIMLLSDRNSRFACANT
                     DNIDLPLSKFERRFRQAGAPIYRLALRKTSPVT"
     gene            complement(13183..13494)
                     /locus_tag="VFO30_06375"
     CDS             complement(13183..13494)
                     /locus_tag="VFO30_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET9418949.1"
                     /translation="MCARCDDERHVTRHLHGECRTERGPHRLGIIFLIASMAGAGASR
                     MWFRIFGVVYALVAILGFMTRNGMLLGMISNNVADTWLHVILAVAMLFLGFVAKDTAT
                     A"
BASE COUNT         2866 a         4011 c         4040 g         2906 t
ORIGIN      
        1 ctcgacgtgc ggcagttcga gcgcgcggtt tacccaaccg ccgcagcacc atttcggcgg
       61 gcgaatgtcc cagtcgggat gattatcgaa ggacacgagc acaactcgcg gtctaacacg
      121 ccgcagccaa agcgccgtca gataatgaaa atcgccggaa ccatagatca gaaacggcgc
      181 aacctgatct ttgatctcgg cataaaactc ttcgaccagg cgccgaggcg cggaaaagcg
      241 caagcgcggc ccccatgcct gggcatcgag cgccggcaga cctaacggtt catccagcca
      301 ggcgtcatca agattcacat gtacggccgg cactggcacg ggtcattgtc gatctcgtgc
      361 gcgcagtttc aaagttgcgc gcgaatttaa ggtgaccccg tggaaaagac agaaaaacaa
      421 gagcttaaag aacacgaaat cgagcgcgat tttttttgtg tgatccggga tgctttccgg
      481 gttttcgccc agcgcgcgtc cattctcgtc ggcacgccat gggtcttcgc gcttgccatt
      541 ctcgtcatcg tgatctgggg gatcacgggc ccgaagttcc attactccga cacgtggcaa
      601 ctcatcatta atacaggcac cacgatcgtt accttcctga tggttttcgt aattcagaac
      661 acgcaaaatc gcgacgctaa agccattcac ctaaagctgg acgagctcat tcgggcgata
      721 aaaggcgcgc gcaataaact tgtcgatctt gaggaacttt cggacgacga attaaaaaag
      781 ctcgaacaag aatttcagcg gctgcgcaaa aagtcagccg agaccgacac ccgagtggcc
      841 aaggtcgaga cgcaacagag gaactcgcgc taggttgggt tcgcctcagc gcgcgcctgg
      901 ccggttcgca cgaacacttt tccgctcggg catagccgga agatttcgca ctggcggcag
      961 tctggctttc gggcaaagca gcggcgccgc ccgtgccaga tcagccaatg gctccagttg
     1021 gtccagtgct cgcgcggcac cagcttcatc aaatcgcgct cgattttgtc ggggtcggtt
     1081 tgtttcgtta ggccgagccg ctgtgacagc cgcgcgacat gcgtgtcaac gacaacgccc
     1141 tcgtttttgc cgaatgcgtt gcccaatacg acattggccg tcttgcgccc aacgcccggc
     1201 agttggcgta acttctccat cgaatttgga actttgccgc cgaacttgtc gataattgtc
     1261 gatgttgccg cgcgaatgct ctttgtcttg ttgcgaaaaa agccggtggg tttgatcatc
     1321 ttttccagcg cagccggcgg cgcgctcccg taatcgttag cggtgcggta ttttttgaac
     1381 aaagccggcg tgaccatgtt cactcgtttg tccgtgcatt gtgccgagag aatcgttgcg
     1441 atgagcaact cgagcggatt cgtgtagtcc agctcgcagt gagcacccgg ataaacctgg
     1501 ggccagactt cgatcaattc tgtcacgcga tcgtgcgccg tcatctcgct ggagtgccct
     1561 tcacgacgcg tctgacgcgt ccgacaagaa tccggccgcg ctttataacg cgacgcgccc
     1621 aaatgaattc actcgccaga atcgcgaatc cgatcgggat cacgatgacg gcaggaccgg
     1681 gtaagaccac caaagctagg ccaatcaaga gcactgtcgt gccaatgact gcgaccacaa
     1741 ttttgcgaag cagttgcggt gcctgttgca gacgcaagcg tgcgacgaac tcccgaatcc
     1801 gctgcatcgg ccaacatgcc ggcaactgtt cgatccgcaa tcaaacaagc agagtggcta
     1861 ggcggagtac tgttcgagct ttgcttcgag atgtggcggt acgtgggcga caaggaccgc
     1921 gttatcgcct tcatatttca gctcgagcac atggccgacg cggtgaatct cggccagcag
     1981 cgccgattct ttcgccggaa ttcgaaaccg cgagcgcaag cgccatgcgc tcaacgcgtc
     2041 ctgcagcgcc tgcacgagct tgttcacgcc ttcgcccgtg cgcgccgaga tcgcgacgct
     2101 gcaggaaaac cttttcacat aggtatcggc cagctcgcga tccgggagat tgtcgatctt
     2161 gttgaagacg atcagtgttt gcttgccgta cgcgtccagc tgcttgatca cattatcgac
     2221 cgcttccatc tgttcttcgg cgcgcgggtg actaagatcg acgatgtgaa tgagcaggtc
     2281 ggcctcgctt acctcttcga gcgtggcctt gaatgactcg atgagcgtgt gcggcagttt
     2341 gcgcaaaaaa ccgaccgtgt cagttaacag aacccgctgt ttgttaggca aaacgaaact
     2401 gcgcgtcgtc gggtcgagtg tggcgaagag tttgtcctcc gttgtgacgt ccgcgccggt
     2461 cagcaaattc aggagggtcg atttgccggc attcgtgtag ccgaccacgg ccgcgaccgg
     2521 ccattgatgc cgctttcggc cctgccgctg cacggcgcgc gttttgcgga cggcttccag
     2581 ctcgcgctcg agtcgggcaa tgcgttcctg cacccgccgc cggtcaactt cgagctgcgt
     2641 ttcacccggc ccgcgcgtgc caatgccacc agtctgacgc gacaggtgat gccacattcc
     2701 cgtaagacgc ggcagcaaat actgcaattg cgcgagctca atttgcaatc gcccttcccg
     2761 gctgcgcgcg cgttgcgcaa agatgtcgag gatcaactgc gtgcgatcaa gtactttgcg
     2821 cgcgagcagg ttctccaaat tgcgcccctg cgctggagaa agctcatcat cgaagattac
     2881 ggaagtgact tgtcgatctt gcaacgcgtg cttgagcgac tcggccttgc cgcggccgat
     2941 gtaatacggt gccgtcggct tttgcagttt ttgagtgact gtgtcgacga cttccgcgcc
     3001 ggcggagttg gccaactcgg ccagttcttc catggaatcg cgcaggtccc atttcgagac
     3061 gccctccttc tcgaggccga tcagcagcgc gcgttcccgc tttctctgcg gagaagtttc
     3121 aatcatcccg tcgcgcgtga gagcgctgcg ctctcagcga aatccagtgc accgcttcct
     3181 tgtgggtcag gccggataag ttcagcgggg gaaaattagt ttggcgccgg aaccaggtca
     3241 actggcgctt ggcatagcgc cgcgtcgcct gctggatcgc ggcgatgcat tgtaacatcg
     3301 acattttccc ggcgagcagc tgccgaatct cacgcacccc aatcatcttc gaagcagtct
     3361 cgctcatcgc gcccactgct cggacttctt cgatgatgcc gcggttgaac atcgcttcca
     3421 cccgccggtt gattcggtcg taaagctccg cgcgaccgcg caacacgaac acgcccgcag
     3481 gatggacatc gactttcttc cagtcgcgat gcaagccatg ggtcagcgcc ttgatgtaca
     3541 gaccactgcc accgacgacg agcgcagttt ttccgcggct gtggatttcg gctattgcct
     3601 ccagcgccgc gcgccgaaat ttctccgcgt tcatctcttc caagatcgac accatgccga
     3661 taagatgatg ccgcgctttg gcgagcgtag ccgcgtcggg tttcgccgta atgacttcaa
     3721 agccctcgta gatttgaaac gcgtcggcat tgacaatctc gacaccgagc tcgcgcgcca
     3781 catccgcagc gagctccgac ttgcctgatg cggttggacc gacgatgaag aagacccccc
     3841 gcatgcagca aataaatgcg acggtcatgg accgccgcta gaggaaacgg aaaccgggag
     3901 acgtgctttc gcttcgtctc cggttcccag attccagcca aatgctgtta gctgtgcgac
     3961 tggcgctcag tcgatttcgc gccgctgacg acgagcttgc gaccgaggaa ttccttgtcg
     4021 tgcaattcct ccaccgcgcg tttggcttcc tcgatcgtct gcatgtggac gaaagcgaaa
     4081 cccttcgagc gctgattgtg acggtagctc acgatctcgg cgttttgcac gtggccgacg
     4141 ccgttgaaca gctcgaaaag gtcactttcc gtcgcgtcga aggaaagatt gccgacgtag
     4201 agacgcggcg aagtaacttc gaccggctcc ggcttacgct gcggccgcga ggctggtgcg
     4261 ccattgcgcg ccgccattgc cggcgccctg gccgtcttgc cattggcgaa aaacgcagcg
     4321 agccgctgcc agaatgtttt cttgggcgcc ttcgccggcc cgctgtcttg acgccgacgg
     4381 cgatcgcccc gatgacggga accgccgcgc gaacgacgcg agcgtccccc cgatgaatgc
     4441 gagttcatga ttgtccttta gctttacccg attgttcgtt gggcagagcg ccccgccatc
     4501 attcgggaca ccgtccttgg cgcgcaaacc tcgaaaggtc cgttgaagca aaatgcatat
     4561 acgtcgcgac gccgtcggaa actccgcctt gttgtgccgg gaacacgccg ccgccaggga
     4621 acgaaacagt aacgatgcac aaactggcat catggattcc aagaaacctc ggtcgttatg
     4681 gcaagtggac gtccgaatca tcgaacgatt cccgacatct gtcgccgagt atcgtctgcg
     4741 gggcaggcga ttgcaagtga ggtttagcgg cggacccagt cgtcacggag cgagtagaag
     4801 cgggatttga gatactgttt cgggcgctcg agccggactg gcttgaagaa gtaacgcagg
     4861 gcacgtcggc tgagcttcgg ctcatagtca aaagtcggca tcatatcgcg gcaatgccat
     4921 tctacccagc cgatctcctc ctccgacagg tcgctctccc acctcgtcaa cggctcggcg
     4981 ttaatgtcca caaacgaggt ctcggctgca gaattaccgg cccaaaattt cccggctttc
     5041 gtcggcgcga ggacgatggc cggatcgtaa gcaacttcga gaaacgcgca cacccttttc
     5101 atcacgccag cggtgtcgag cacgagcttt tcgtacggaa cggcgagcgc gtcgggatcg
     5161 tagccgcgca tcgcgcgaag tgcagtcgtc gccgcctcgc gccagtggct gatgcagtag
     5221 taggtcgaaa aagtgccgag gcgccgcgtt ttctcgaggg cgatttgcgc ggcgagaatg
     5281 gcgcgaggat cacgaatcgt gaccagaacc ttggcgtgcg gaaaatgctg ccggatcgcc
     5341 ggtaaatatc tgcggtttgc cggcgtcttt tcgatccaac gccggatcga atcgagtggc
     5401 cgaccgagcg tcattgcgta cgcttccatg aggatgacga gcaaatcctt gtcggcgttg
     5461 accggatcga acgccatccg ctcgaaggtc tcgagaaaag gcgccgtcgg aaaataggag
     5521 taatcgcgtt tgccccattt gcatttgccg ccgaacagga cgtttgagag cgattgcttc
     5581 gtgatgtagt cgaactgcgc gcgccggccg cggggcgcgt actttgtcag gaccgttggg
     5641 aagtacgccg tctcttccgg caggacgagc aactcgggat ggctgtcgag cagtgcaacg
     5701 agcaaagtcg tgcccgattt cgccgggcca gcgatgaaac aggcacgctg atcgaacgga
     5761 agaaacgaag tcatcgaaac caaattacaa ccttaaatgg ccaatcatag tagcgcagcg
     5821 ctcatacagt agcgcaagcg tcccgcttgt agtcacgact tcgcaagcca gaggcttacg
     5881 ctacttttcg ttacgcagaa accagtcgat gaatcgcggc aagccgtctt tcagcggcgt
     5941 cgttggattg tagccaagta gtgctttcgc tttcgaaatg tcagcacacg tgagcggcac
     6001 gtcgcctggc tgttccggta agcgattgat cttcgctttc tttccaagcg cgttttcgat
     6061 cgcggcgatc aagtccttga gctggatggt ttcgctttcg ccaaggttaa aaatgtcgaa
     6121 caatgcgccg tcgtactcga gtgccgccat cgtgccttgg ataatgtcat cgatgtaggt
     6181 gtaatcgcgg cgtgtggtgc cgtcgccgaa ctggtcgatc ggtttacccg cataaatttt
     6241 tcgggtgaac tgatggatcg cgaggtcggg ccgctggcgg ggcccgtaaa ccgtgaagta
     6301 acgcaacgcc acgacgcgga tgttgtacag gtgcgaaaac gtggagcaga gaaattcgcc
     6361 cgcgattttc gttgcggcgt agggactaat tgtctgggtt agatgctgat cttctgagaa
     6421 gggcaccgtt ttcgagattc cgtagacgga agagctggag gcgaagataa agcgcttaat
     6481 cccacttgcg cgcgccgctt cgaggaggcg aagcgtgccg acgacattag tgtcgtaata
     6541 aagttgcggc tgcgcgattg atggacgcac cccggcgcgc gcggccagat gtgcaatcgc
     6601 gtcaaagttt tcgctcccca aaacatttcg aaccgctgcg ccgtcgcgaa gatcgacatg
     6661 atgaacgcgc acgtctctca caatcccggc aatgttcgtc cgcttgatcg ccggatcgta
     6721 gaaatcgttg aagtcatcca gaatggcgac ctcatggccc gcggcgagta gtttctcggt
     6781 taaatgcgat ccgataaacc cagcgccacc agtgacaagc acccgcatat tggcagaagt
     6841 tcaaccacga agcacacgaa gtgcacgaag aaaatggccg tgggcggttg ctcgatcttt
     6901 tcgtgttctt cgtgatcttc gtgttaacga atgaggaaac ttttcggact cgccgtaggc
     6961 gcatttgcgc ttggtttcgt tgctatcgtc ggacttcagc tttggccgga actaacgcgg
     7021 tccaaatatg tcgatcttgc gtttcgtatc ccctcgcctt tcgaattggc gtccctgccg
     7081 atcgcggcgc ggtttgattt tccactcggg agcgagaacg gcgcgctcgc ttacaacgcg
     7141 cagcggttca ccgaaaatcg ccaccttggc gacgatctga atggcatcgg cggagagaac
     7201 agcgacttgg gcgatcccat ttactgtgtg gctgacggtc gcgtgttact cgcgcgcgat
     7261 ggcggaactg gttggggcaa tgtcgtcatc gtgttgcacg cgtatcttga aaatgggggc
     7321 cggaattatg tccagtcgta ttacgcacat gtggaaaaga tgctcgtcca tccgggcgat
     7381 gatgtgcggc gcgggcaaca gatcgcgacc gtcggtaccg gcggcggccg ctacttcgcg
     7441 cacttacatt tcgagatgcg cgaatttgtg acgccgttca tcggccccgg ttatcgcgaa
     7501 gacacccgag gctggctcga cccgagcgct tttattgaag cgcaccgcgg ggcgccggaa
     7561 gaggatgtcg gccgcgcccc gctggaatga acgctgctaa aacttctgcc agtgcgcgag
     7621 caagtacatc cagttcagtg caagtgcact taccatcgtg cctcgaccat aattttgtgc
     7681 ggccgcagaa aaactgatgc gcaatcgagg agcgcgcgtg atgacgacag caaacgcgta
     7741 gaagtcgaag atcgacaatc cgcaaagact gacaaaaatc agcggattcc agttgaacgc
     7801 gcggagtaag tctccgtgaa aaaaggcgat cgcggaacgc gtcatgccgc acgtgatgca
     7861 cggcaaaccc gtcacgtgat ggaacacgca ttgcggccaa ggcaatccga aaccaagcca
     7921 tgctccggca caggcaaaag aggccaacga cacgctgagc cagattagct cgtgatcgag
     7981 ttcgtttggc gcaagctgtc gtcgatggaa ctgcatgagg cgacctatcg atttccgaaa
     8041 tgaatcgcgg tcgggatcag gatcgccaac agcagcacgc caccgcaaca taccatcaga
     8101 ggaaggaaaa tcgcgagcac ggcgcggcca gtttcggtct catgcgcgcg cgcgagcccg
     8161 atgcaattca ggaccaatgc ccaaacgccg gcgatgatgc cgccacaaat cgggatcatt
     8221 tgcagcggcc cggttgagcc ttgggtgaaa gcgagcacgc gcagcgttgt ttcaaatgtc
     8281 ttgttcgcgc cgcccacaat catcaggcag aggtgcacga tcgcgccgcc gatgaataga
     8341 ccgaggacga tgacgatcgg caggaggata atgaaaacaa ccgagccagc gccgattccg
     8401 gtcagccaag cgaaggtgtt gtggcggccc accgtcagac cgatcgattg cagcgctaac
     8461 gaaaagagaa atgatacaat cccgccggcg catccgccga tcagggcgta aatgatcggg
     8521 ccggctaggc cgccctccac tttcatgatg cggaaggcgt aatccggccg cgtcaggacc
     8581 gtctgcaatg tttcgatgaa ggcattaaac cagcccttct ggtcgcgctc atcccaaggc
     8641 aacccagttc tagacaccgg aacttcggat gccgctgggc tcaccgacgg cggaactgtc
     8701 gatggtgcga tcgatggagg aggcggtgcc gcaccggccg tttccgccgc aaattcctca
     8761 aactgcgata aaggctgcca gttctgcatt ccttcgcgcc agccgagatc gctcggcatg
     8821 aaacggctgg tgcgcagtcc ttcgcgcaca tcttcctccg aaaacgcacc gaaggtcgtc
     8881 gcaccgcgac tcacgtgaat catcgccata atcgcgcgtt ttagttaggc gcgtcggcaa
     8941 agtcaatctc ctcgacgatg atgtctattc gtctggatta accgggaagt gtatgttctt
     9001 gaggtgctcg ggaacgcgct gcgtttgctc gctctcgatc ttttcggtcg cggcgttgag
     9061 cgcgtcgtag gcttcgcggc tcttgttcgc gcggcacatg taagcggttg cgtgcgcgag
     9121 cggaatccgc gcttcaggta aaccaacgaa ctcgacggcc tgctgcgtcg cgatcgcgag
     9181 cggcaaggct tccggatcag ccagaccgac gtcttcgctc gcaaagatta cgatgcggcg
     9241 cgcgataaac cggaagtctt ccccggcatg cagcattttc gcgagccagt acatcgcgcc
     9301 tggttcatcc gacgcgcgca tgcttttgat aaacgcgctg atcgtatcgt aatgcgcgtc
     9361 gccggcgcgg tcgtaaacga tcgctttctg ctgaatgctt tcctcggcaa cggcaacggt
     9421 gaaatgaacg acgctatttc catcggccgg cgttgttagt acgccgattt cgagcgcgtt
     9481 taaacatttg cgcgcgtcgc cgtcgcttag tttcgcgaga tggcgccgcg cgtcaccgtc
     9541 catctttacg ttgtaattgg cgaggccgcg ttctttgtcg gcgagcgccc gatcgatcag
     9601 cagttccagt tggttgacac ggagcggctc gagctgaaaa acctgcgacc gcgagacgag
     9661 cggactgttg acgtagaaga atggattgta cgtggtcgcg ccgatcaagc gcaggtttcc
     9721 gctctcaaca tccggcaaca aaatgtcctg ctgcgctttg ttgaaatgat ggatctcgtc
     9781 gataaacagg atcgtcttct gacccgcgtt gcggagccgg ttggccgcgc cggcgacgac
     9841 ccgccgcatc tcggcgacat tgctctccac gccgctcaac cggacgaact gcgcatgcgt
     9901 catgtcggca atgatgtgag cgagggtggt ttttccggtg cccggcggcc cggaaagaat
     9961 caccgagggc aagcgatcgg cttcaattgc gcggcgcagc aactttcccg gcgcaagaat
    10021 atgttcctgg ccgacaaatt cgtcgagtga ccgtggccgc attcgcgtgg ccagcggtgc
    10081 ggctgagttc gtcgccgcaa gatcgacatc ttcttcgctg aaaagatcgt gcaccgccga
    10141 aactatgctg gcggggacag accctatgca agtgcggctc gccgagcctc agaaacttcc
    10201 gcaccgcatt tccgtagaac agaccgcacc catgcctctg atcgggagcg atgtaacgag
    10261 ccggccaagc cgccattggc gggcgcggca gccgttcgtt ggattagtga tcgcagccgt
    10321 ggctggagtt ttgctcgcag actatcttgc gatcccttgc actgcatggc tgccgatgac
    10381 tggtattttc gccctggcag cgatcgccac ttattgctgg ccgcatgcaa catcgactcg
    10441 tgcgctggtc ggatttggtt ttttcttact ccacactttt cgcacgcacg acacgccggg
    10501 tcagcggctc gtcgaacggc ttggcgaccg tgctcgcgtg atcaccgcaa tcggaacggt
    10561 gacgagcgag ccaaaggttg cgcctaacgg atttgcaacg ttcctggtaa ctctggaatc
    10621 agttcagcta gaggacacaa ctgtccgtag ccgcgccacc tgcctggtac gctggcgtgg
    10681 cacgccagaa tttggaaacg agctcaaagt attcggaatc gccgaaccaa tcccaccacc
    10741 gcgcaatcca ggtgaattcg acatgcgcgg ctatctcgcg cgtcgcgacg tgcgccggct
    10801 cttgttcgtc cgctatccgg aagacggcgt cctgctgcga cacggcgcgg gtaatccgat
    10861 cttgcgcctc gctcaaaaat ctcgtgcgtg gatgcaattg gcattgtgcc gcggtttgga
    10921 gaacgccccc gacgtacagg cgttcctgag cgggatcgtg ctcggccagc ggcatcaaac
    10981 accggaggac attgaggagc cgtttcaaca aaccggcaca ctgcatatat tcgccgtcgc
    11041 ggggctgcac gtgggaatcg tggcgcggct tttgtggatg ctcggggtcg tcgctcaatt
    11101 gccgcgcaaa ttggcaacgg cgctgatcat tccgctgctg ctgttttatg cagcgattac
    11161 cggcttacac gtctcaagcg tgcgcgccgc ggtgatgagc tccgtgctgc tcggcggctt
    11221 ttttgtcgag cgcaaggtgt ttagtctgaa tagtctcgca gctgctgcgt ttctcatctt
    11281 gtgctgggat acaaatgaat tgttctccac tggttttcag ctgtcgtttg cggtcgtcgg
    11341 agcgatcatc ctgttcagtc atttgccgtt cgcgtatctg tggccattgg ctgcgcccga
    11401 cgcatttttg cccggagcat tgatccgcgg tgcgcggcga tggctgcaca ccgggttgga
    11461 gtggctgtgt cgcgccgcat ccgtgtcgtt cgccgcgtgg gctgggtcac tgctgttgct
    11521 tctctggtac ttctatctcg tgacgccgat ctcactagtc gccaacctga ttgtcgtgcc
    11581 gatggcgttt ttcattctcg cgatcgcgct cctctcgatg gtggcggcac cgttccttcc
    11641 gtcgttctcg atcttgttca ataacgccaa ttggtgcctc gctcggctcg tcctcggcat
    11701 cgtccatctt cttgcgcagc tgccgggtgg gcattactac attgcgcatc cgcgttggcc
    11761 tgcgaacgcg acggcgacca tcaccgtgct ggatgtcggc accggagcgg ccattcacgt
    11821 gcgaagccgt ggccaggatt ggctgttcga ctgcgcaagc gaacgcgact accagcgtgt
    11881 cgtgcggccg tatttacatg ctagcggtgt taacggtatc gatggtgtgc tgttgacgca
    11941 cggtgattcg ctgcacatcg gcggcgcgtt aggcttgctg gaggactttg cacgaccgca
    12001 gctgatcgac aatccagcgc cggaccgttc gatcatacac cgtcgcctgc gcaccatttt
    12061 tcagcaacgc aacctaagcg tccgtaacat ggttgctggt gagaccttta cgatcgcgaa
    12121 cgaagtcacc gcgcgtgtgg tgcaccctcc gtccggattc gccgaatcca atgccgatga
    12181 tgaagcgtcg gtggtccaag tagctgtgtc accaatgacg aaaattctac tgacttcaga
    12241 tgccggtctg ccgacggaaa atgcgctcct cgcttccggc atcgatttga aaagcgacat
    12301 cctcattaaa ggcgaacatc gctccgccac gtccggctcg acgcggttcc tcgatgctgt
    12361 taggccgcaa ttgataatcg caacgtcgcg ggattttccg gagtccgaga gaatctctga
    12421 cgattgggcc gcccgcatgc gggcccgtgg gatcaaacta tttcggcagg acgaaaccgg
    12481 cgcggtcgag ctgcagttcg gccggcgaga atggagcgcg cgggctcacg tcacaggcga
    12541 agtttttcgc agcgccaacc gataaatcgg cgcgccggct tgccggaatc gtcgctcgaa
    12601 ttttgagagc ggaagatcga tattgtccgt gttggcgcag gcaaatcgag aattacgatc
    12661 gctcaacagc atgatttggc gaaagtaatc ccactgatcg gttgcgactt tgagcagccc
    12721 ctcatcgaca agcgcggcgc ggattgcatc cagaaaatcg gaggtaacga ctcgccggcg
    12781 gtagtggcgg cgttttggcc agggatcggg aaagagcagg taaaaaaccg caactgagct
    12841 ctccggaaac aagtagcgga ccgcgtaaga agtgtccaga tggaggactc gaacgttctc
    12901 gagcgcgcgc gcccgtcggc aggctttgtc cacgcgtccg ctcattcgct cgatgccaag
    12961 aaagttttca tcgggatgga gctcggccag agcgcagaga aaggagccgt cgccacagcc
    13021 aagatcgaca tgcaggagcg caacgcgacc gaagatcttt tccagattga gccgcgcgac
    13081 gtacgagttc gggatcagtt cgatcacggc aaaaaaatgc gccggcggct atgcgattcc
    13141 aatcgcgttt gccgcccggc ttcccaggga gtctgaaaaa ctttaagccg tagcagtgtc
    13201 cttcgcgacg aagccgagaa agagcatggc gacggcaaga atcacatgca gccaggtgtc
    13261 agccacgttg ttcgagatca tgccgagcaa catgccattc cgagtcatga agccgaggat
    13321 ggcgacgagg gcgtaaacca cgccgaagat gcggaaccac atccgggagg cgcccgcgcc
    13381 ggccatgctg gcgatgagga agatgatccc gaggcgatgt ggaccacgct ctgtgcggca
    13441 ttcaccatga agatgccgag taacatgtcg ttcgtcgtca cacctggcac aaatccgaga
    13501 attcccacag ctagaaacac gataccgaag acgattgcgg cagctttaac catatgacct
    13561 cctcgcggtt aaggcgacaa gcttattggc agcctggcgc cgacgtggca agccaaaaaa
    13621 tggatatccc cgtcaccgat ccagcgtaag attgccaaca tgaggcgaac tgttattgcg
    13681 gcaatctcgg tatggatcgc gctgctgatt gtcgatctta caaatggaca aactcccacg
    13741 ttcacacgga caaaaaccgc cgtgctcgcc ggaggctgct tttggtgcat tcaacccgcg
    13801 tttgataaag cgtcgggcgt aat
//
LOCUS       DASTDG010000199         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_642602, whole genome shotgun sequence.
ACCESSION   DASTDG010000199 DASTDG010000000
VERSION     DASTDG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642602"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFO30_06380"
     CDS             complement(<1..484)
                     /locus_tag="VFO30_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9418950.1"
                     /translation="MNIPAEKAALDGDLTIVEQATGIVLFAHGSGSSRHSPRNQFVAR
                     TLNNAGLATLLFDLLTPEEELIDMRTAELRFDIDLLAGRLVDATRWTKLQPATRDLRF
                     GYFGSSTGGGAALVAAAQLPNDTGAVVSRGGRPDLAGDSLGKVKAPTLLIVGGEDHVV
                     I"
     gene            complement(498..1181)
                     /locus_tag="VFO30_06385"
     CDS             complement(498..1181)
                     /locus_tag="VFO30_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET9418951.1"
                     /translation="MRAFSNRAEAGQLLAEKLLKYGGREDVIVLGLPRGGVPVAFEVA
                     QRLRVPLDVFIVRKLGVPGFEELAIGAIASGGVRVLNEDVARVLPNMDEIIEAVTARE
                     TAELQRREHSYRDGRRAPQLRGKTAILVDDGLATGATMRAAVKALRQRDVGKIVVAAP
                     VGAPETCREFEDEVDEIVCPLAPEFFQAVGQYYEDFSQTSDEEVRELLARAASAAGPL
                     QSGEERHSA"
     gene            complement(1300..1818)
                     /locus_tag="VFO30_06390"
     CDS             complement(1300..1818)
                     /locus_tag="VFO30_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418952.1"
                     /translation="MIKRKTVNLEAVNALAQLIAAIGVIASLFYLAAEIRQNTRSQRM
                     AVVDSLAHSIADLLVPLSHNPELMRAFSAVAENWYGATEEDRLRVLPFNLALFKLFEN
                     AWFQKRQRTLDPQQWEGWDLYIRMYYHRPGMKDWWRLRRAAFAPGFRDYVESSAPIEE
                     LPSVTELIRGDR"
     gene            complement(1915..>2946)
                     /locus_tag="VFO30_06395"
     CDS             complement(1915..>2946)
                     /locus_tag="VFO30_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9418953.1"
                     /translation="TPPDGITAEVIPVKNFEELKALPRDKVAGKIVLFNYPFDKQMAA
                     EGRGGEAYGEAVVYRADGPSAAARQGAVACLIRSVGGADYRLPHTGQTNYANDAPKIP
                     AAAVTAEDAELIVDLVRQGPVKMNLVLTPQTLPEVESANVIGDIKGSEHPEQVVIVSG
                     HLDSWDLGTGAIDDGAGVAVSMEAANLIRKLGLKPKRTIRVIAWMNEENGLRGSKQYA
                     KDQEKAWANHFAAIETDGGAGHPIGINIKGKPELKAILKSVAAILQESGAGILNLVEH
                     CGADIEPMEKAGIPTFAPLQDSRFYFNYHHTAADTLDKIVPKELAENSAVIAVLAYAL
                     ANCEQPLPK"
BASE COUNT          575 a          932 c          812 g          627 t
ORIGIN      
        1 cgatgacaac atgatcttca ccgccgacaa tgagcaacgt cggcgctttg actttgccta
       61 acgaatcgcc ggcgaggtcc ggccggccac cacgggaaac gaccgcgccg gtgtcgttgg
      121 gcaactgcgc tgccgcgacg agcgcggcgc ctccgcccgt gctcgagccg aaatacccga
      181 agcgcaaatc gcgcgtcgcc ggctggagtt ttgtccatcg cgtcgcgtcc accaagcgcc
      241 cggcgagtaa atcgatatcg aagcgcagct cggctgtccg catgtcgatc aattcttcct
      301 ccggcgtgag aaggtcgaag agcaacgtgg cgagtccagc gttgttgagc gtgcgcgcga
      361 cgaattggtt ccgaggactg tgccggctgc tgccgctgcc gtgagcgaaa agaacaatcc
      421 cggtcgcttg ctcgacaatc gttagatcgc catcgagcgc cgccttctcc gctggaatgt
      481 tcacttccat actaaactca tgcagagtgt cgctcttctc cgctttgcaa cgggcccgct
      541 gcggacgcgg cgcgcgccag cagctcgcgc acttcttcgt cgctggtttg cgaaaaatct
      601 tcgtaatact ggcccacggc ttggaaaaat tccggcgcca gcgggcagac gatctcgtcc
      661 acttcgtcct cgaactcgcg gcacgtctcg ggcgcgccga ccggcgcggc gaccacgatt
      721 ttccccacgt cgcgctgccg caatgctttc accgcagcgc gcattgtcgc gccggttgcc
      781 agtccgtcat ccacgagaat ggcggttttg ccgcgcagct gcggagcccg gcgcccatcg
      841 cggtaactgt gttcccgtcg ctggagctcg gccgtttcgc gcgctgtcac tgcttcgatg
      901 atctcgtcca tattcggaag aacgcgggcg acgtcttcat tgagaacgcg aacaccgcca
      961 gaagcgatcg cgccaatcgc aagctcttca aatccgggca caccgagttt gcgcacgatg
     1021 aaaacatcga gcggcacgcg caatcgttgc gcgacttcaa acgccaccgg cacaccgcca
     1081 cgcggcagac ccagaacgat gacatcttcg cggccgccgt acttcaggag cttctcggcg
     1141 agcagctggc cggcttccgc gcgatttgaa aaagctctca tctcattccc accgcaacct
     1201 tgatgccgct caaacgcgag ccctttctgt ctaatatgaa ttcggatttc gcaggagcga
     1261 tggccccagc agcaaaggag cgcactggtt gttctgcggt cagcgatcgc cgcggatcag
     1321 ttcagtgacg gaaggaagct cctcgatcgg cgcgctgctc tctacgtaat cgcgaaaacc
     1381 gggcgcgaac gcagctcttc gcaatcgcca ccaatctttc attcctggac gatgatagta
     1441 catccgaatg taaagatccc aaccttccca ttgctgcgga tccagagtgc gctgccgttt
     1501 ttggaaccac gcgttctcga aaagcttgaa tagcgcaaga ttgaaaggca gtactcgcaa
     1561 tcgatcctct tccgtcgcac cgtaccaatt ttcagcgaca gcagaaaagg cgcgcatcaa
     1621 ctccggatta tgcgacagcg gcacaagtag gtcggcgatc gagtgcgcga gcgaatccac
     1681 caccgccata cgctgcgatc gcgtattttg ccgaatctct gcggccagat aaaacagcga
     1741 agcgataacc ccaatcgcag caatcaactg ggctagcgca ttgaccgctt ccaggttcac
     1801 ggttttccgt tttatcaaag agcccgacaa cacgcgagtc caacgacgat gacaatctta
     1861 gtagccgtcg ccctgcgggc gccgcgctcg tttcgcccag cgaaacggct acagttattt
     1921 tggcaacggc tgctcgcaat tcgcgagcgc gtaggcgagg acggcgatga ctgcggaatt
     1981 ttccgccagt tcttttggca cgatcttgtc gagcgtatcc gcggcggtgt gatggtagtt
     2041 gaaatagaat cggctgtcct ggagcggcgc gaacgtcggg atgccggctt tctccatcgg
     2101 ctcaatgtcc gcgccgcaat gctcgaccag attcaggatg ccggcgccgg actcctgcag
     2161 gattgcggcg acactcttca gtatcgcttt gagttctggt ttgcctttga tgttgatacc
     2221 gatcggatgg ccagcaccgc cgtcggtctc aatcgctgca aagtgatttg cccacgcctt
     2281 ctcctgatct ttggcgtact gctttgagcc gcggaggcca ttctcctcgt tcatccacgc
     2341 aattacccga atcgtgcgct tcggtttcag tccgagtttc cggatcaaat tcgcagcttc
     2401 catggacaca gccacgcccg cgccatcatc gatcgcgccg gtgccgaggt cccatgaatc
     2461 aagatggcca gagacgatca cgacctgctc ggggtgctcg ctccctttaa tgtcgccgat
     2521 cacgtttgca ctttccacct ccggcaaagt ttgcggggtt aataccaggt tcattttcac
     2581 cggaccttgc cgcacaagat cgacaatcaa ctctgcgtct tcggccgtga ccgccgccgc
     2641 tggaattttt ggcgcatcgt ttgcgtagtt tgtctgtcca gtgtgcggca ggcggtagtc
     2701 ggcgccgccc actgaacgga tcagacacgc aacagcgcct tgtcgcgcgg ctgcgcttgg
     2761 accatcggct ctatagacaa ctgcctcgcc gtatgcttcg ccaccgcgac cttccgcggc
     2821 catctgtttg tcgaatggat aattgaacag cacgattttg ccggcgactt tgtcccgcgg
     2881 cagcgccttg agctcttcga aatttttgac cgggatcact tcggccgtaa ttccatccgg
     2941 cggagt
//
LOCUS       DASTDG010000200         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1330509, whole genome shotgun sequence.
ACCESSION   DASTDG010000200 DASTDG010000000
VERSION     DASTDG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330509"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1144)
                     /locus_tag="VFO30_06400"
     CDS             complement(89..1144)
                     /locus_tag="VFO30_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9418954.1"
                     /translation="MTKPFDWWPANTALSFVRPWLLLLLLAIPLLAYLLGKRGPAAAL
                     TFSSTASLRAIGKQSAARAGKFLRALVFAALAFFVVALARPQLGKTLTEIEASGIDIM
                     LVLDVSGSMLIKDFTIGGEEATRVDAIREVTRKFIEGRPNDRIGIIAFGGRPYVVSPM
                     TLDHDWLLQNLDRVRIGLVEDGTAIGSAMAVAANRLNDKHSKSRVIVLLTDGENNAGK
                     ISPETAAEAIKALHLHFYAIGAGINGIAPVPVFTARGPLTDTDGNILYENQPVRFNEA
                     GLRQVANIADGKFFRATDTKSLQRIYDDIDKLEKTTVNVKKYQQYRDLFPLCIMSGAG
                     LLVGQILLSQTIWKKLP"
     gene            complement(1276..1812)
                     /locus_tag="VFO30_06405"
     CDS             complement(1276..1812)
                     /locus_tag="VFO30_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HET9418955.1"
                     /translation="MSIFDTPSLAQEFHDIAPPVDYTLVPLWVIFGAIFGGLALVGWI
                     AWLITRKRKRPQPPQLPRDRALQALQRIAAQIEKLTPYQFSIRVSDILRRYVTEQYAL
                     PLTRQTSVEFLNSLRKSSSFSEEEKSLLADFLNRCDLIKFARYDATAEDSRLLLDEAT
                     RFVQGGEVAAALQTSAQS"
     gene            complement(1821..2708)
                     /locus_tag="VFO30_06410"
     CDS             complement(1821..2708)
                     /locus_tag="VFO30_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9418956.1"
                     /translation="MEPTAEILKKIRTLEIRTRGLVETAFAGDYHSVFKGRGMNFEDV
                     REYQPGDEIRTIDWNVTARLGTAFVKKFTEERELTVMLIVDVSASGNFGSSAQSKREL
                     AAEVACLLAFSAIRNNDKVGLILFSDRVELFIPPKKGRSHTLRLIREILFFAPQGGGT
                     DPALALDYLNNVVTRRAVVFFISDFQTPDFSHALAVSGRRHDFIAIHIEDEREEMLPN
                     IGIITLEDAETGEQIEINTADRTTRQRFADLAEARKAELAWTLRRNNIDAIALRTGED
                     YLPALRAFFKQRERRLAIG"
     gene            complement(2692..3132)
                     /locus_tag="VFO30_06415"
     CDS             complement(2692..3132)
                     /locus_tag="VFO30_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9418957.1"
                     /translation="MALAKSFRDLNVYQAAQNAAAAIFQLSKRFPRTEQYSLTDQIRR
                     SSRAVNAMLAEAWARRRYRPAFVDKINQALGEAMETQSWLDHALQCEYLNQTEFSEHD
                     QCWQKIGAMLARMIECADEFCKSPSTINHPRSTAGKPKTDGTDR"
     gene            complement(3176..4162)
                     /locus_tag="VFO30_06420"
     CDS             complement(3176..4162)
                     /locus_tag="VFO30_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9418958.1"
                     /translation="MKSTQEITAEVQQRGQWIPPVREQVAHVVIGQKYLVDRLLLGLL
                     ANGHILLEGVPGLAKTLTVKTMAACIQTRFQRLQFTPDLLPADLIGTLIYNPRTGEFT
                     TKLGPIFSNLILADEINRAPAKVQSALLEAMQERQVTIGEQTYPLSDPFLVLATENPI
                     EQEGTYQLPEAQLDRFMFKLNIGYPQKKEERTILDLMATSAPNLSVRAVVEPQQIIAA
                     RDVVNQIYIDDRVKDYIVDVVWATRDPAAYNLRLEGLIRYGASPRATIYLALGARAHA
                     FLNGRGYVTPQDVKSIGPDVLRHRIIISYEAEAEAVTSETIIERVFAGLPVP"
     gene            complement(4234..5607)
                     /gene="der"
                     /locus_tag="VFO30_06425"
     CDS             complement(4234..5607)
                     /gene="der"
                     /locus_tag="VFO30_06425"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9418959.1"
                     /translation="MPSVAIIGQPNVGKSALFNRLVGRKIAIVHDQPGITRDRLDALC
                     TRGAQPFQLLDTGGIIGAGETELTAQVQRAAQEALRGSDLILFVVDGQQGVSPVDAEL
                     ARSLRRTRKPVVLVINKIDNPKHDALAADFDSLGFDHTVSVSAEHSRGISDLLEKIDK
                     LLPAREIKHQTSDASPAATKPPLAIAVIGRPNVGKSSFINAIVQSERAIVSELAGTTR
                     DAVDISYERNGEQLLFIDTAGIRRRGKHSSSVEVFSVMRAERSIRRADLCVLIVDLTT
                     GVTAQDKRIAGLIQEARTPAIIVLNKWDLFKPKRGEKRTITQLVDETRARLFFIDYAP
                     VLVASAQTGENIDRLFRLVAKIQRESRAHIGTGVLNRRLRTAFAANAPPQKSGKRLKL
                     FYAAQASAKQDRQLPPPEFVLFVNDPRLLTEPYRRYLEARIRQSQRYVGLPISLTLRR
                     REQTRAK"
     gene            complement(5641..6246)
                     /gene="pgsA"
                     /locus_tag="VFO30_06430"
     CDS             complement(5641..6246)
                     /gene="pgsA"
                     /locus_tag="VFO30_06430"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9418960.1"
                     /translation="MTTANKITVLRILMIPAFVTMAIYYGESIQRGAPLEWQRFAAIM
                     IFLLASASDGLDGYIARRYNQRSSLGVILDPIADKGLLLSGIITLSISNWSQTDPEYG
                     RFPVWFPVLVITRDAVILVGAIVLYLLNGKVQVKPNWTGKVATVLQMIAIAWVLLQLR
                     FIPLLAVVISAGLFTLISGVVYVLDGVRQLQAEGHANARVD"
     gene            complement(6341..6940)
                     /locus_tag="VFO30_06435"
     CDS             complement(6341..6940)
                     /locus_tag="VFO30_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9418961.1"
                     /translation="MDKTLAGKKVAILVADGFEQVEMTKPREALQNAGAETKIVSPES
                     GQIQGMHHADKGDKFDVDLTLEEASPEEFDALMIPGGLMNPDQLRSTLEALEFVRHFF
                     NQGKPVAAICHGPWVLIDAGVVRGRTLTSWPAIKTDVVNAGGNWVNEEVVVDNGLVTS
                     RKPDDIPAFNRKMIEEFAEGRHMPRGQQQPAKAAVTLET"
BASE COUNT         1451 a         2026 c         2111 g         1584 t
ORIGIN      
        1 tgcaccaaag ctcataacgt ggcatcggca tcttgccgat gggacatggg ctggaagccc
       61 atgccacgtt gcaagatcga catcttcgtc acggcagttt cttccaaatc gtttgcgaca
      121 acagaatctg cccgaccagc aaacccgcgc cgctcatgat gcacagcgga aagaggtcgc
      181 gatactgctg atattttttc acgttcacgg ttgttttctc caacttgtcg atgtcgtcgt
      241 agattcgctg gagtgatttc gtgtcagtcg cgcggaagaa tttcccgtcc gcgatgttcg
      301 cgacctgccg caaccctgcc tcattgaatc gcaccggctg gttctcatag aggatgtttc
      361 cgtccgtgtc ggtgagtggt ccgcgcgcgg tgaagaccgg cacaggtgcg atgccgttga
      421 ttccggcgcc aatggcgtag aaatgaagat gcagtgcttt aatggcctcg gccgcggtct
      481 ctggcgaaat tttgccggcg ttgttctcgc cgtcggtcaa gagcacgatg acgcggcttt
      541 tcgagtgttt gtcgttgagc cgattggctg cgaccgccat ggccgagccg atcgcggtgc
      601 cgtcttcgac caaaccgatc cgcacgcggt cgaggttttg caagagccaa tcgtggtcga
      661 gcgtcattgg gctcaccacg tacggtcgcc cgccgaaagc gatgatgccg atgcggtcgt
      721 taggccggcc ttcgataaac ttgcgcgtta cctcgcgaat ggcatcgacc cgggtcgctt
      781 cttcgccacc gatcgtgaaa tccttgatca acatcgagcc ggacacgtcg agcacgagca
      841 tgatgtcgat cccgctcgcc tcaatctcgg tcagagtttt gccgagttgc ggccgcgcca
      901 gcgcgaccac gaaaaacgcc agcgcggcaa aaacgagcgc gcgcaaaaat ttgccggctc
      961 gcgcggcgct ctgctttccg atcgcgcgca agctcgctgt cgatgaaaag gtcagtgccg
     1021 cagccggtcc gcgttttccg aggaggtagg cgagcaatgg aatcgcaagc aagagcaaga
     1081 gcagccacgg ccggacgaaa ctgagcgcgg tgtttgccgg ccaccaatca aacggctttg
     1141 tcatgtcgag tgaaagtcga gacacctctc actgtcagaa cgttcgaagc ggcagcaaac
     1201 gctacaagag attcctccac ctcggtcgga ttgacagcca gcacattttc gcaatgaagt
     1261 gcttgcgcgc atcggtcacg attgcgccga agtctgaagc gccgcagcga cctctcctcc
     1321 ttgcacgaac cgcgtcgctt cgtcgagcag caggcggctg tcttcggcgg tggcatcgta
     1381 gcgcgcaaac ttgatcaggt cgcaacggtt gagaaaatcc gcgagcaacg atttctcctc
     1441 ttcggaaaaa ctcgacgatt ttcgaagcga gttgaggaat tcgaccgacg tttgccgggt
     1501 caacggcagc gcgtactgct cggttacgta ccgccgcaga atgtccgaaa cgcggatact
     1561 gaactggtac ggagtcaatt tctcgatctg cgcagcgatg cgttggagcg cttggagcgc
     1621 gcggtcgcgt ggcaattgcg gcggctgcgg ccgtttgcgt ttgcgagtga tcaaccacgc
     1681 gatccagcca accaatgcca gcccgccgaa gatcgcgcca aagattaccc agagcggaac
     1741 gagcgtgtaa tcgaccggcg gcgcgatgtc gtgaaactct tgagctagtg atggcgtgtc
     1801 gaagatcgac attctgttgc ctacccaatc gcgagccgcc gctcgcgctg tttgaaaaag
     1861 gcgcgcagcg ccggcaggta atcctcgccg gtgcgcagcg caatggcgtc gatgttgttc
     1921 cgccgcagag tccacgccag ttctgctttc cgtgcttccg ccaggtcggc aaaccgctgc
     1981 cgcgttgtgc gatcggcggt gttgatttcg atttgctcac ccgtctccgc atcttccagg
     2041 gtgatgatgc cgatgtttgg caacatttct tcacgctcgt cttcgatgtg aatggcaatg
     2101 aaatcgtgac gccgtccgct cacggccagc gcgtgcgaga aatcgggcgt ttgaaaatcg
     2161 gaaatgaaaa acacgacggc gcgacgcgtg accacattgt tcaggtaatc cagggccagc
     2221 gccggatcag tgccgccgcc ttgcggtgcg aaaaagagaa tctcacggat taatcgcaac
     2281 gtatgggagc ggcctttctt cggcggaatg aaaagctcga ctcggtcgga aaagagaata
     2341 aggccgacct tgtcgttgtt tcgaatcgcg ctgaacgcaa gcaggcaggc gacctcggca
     2401 gccagctcgc gtttcgattg cgcgctgctc ccgaaatttc ccgatgcgct cacatcgaca
     2461 atcaacatga ccgtaagttc gcgttcttcc gtaaacttct tcacgaacgc ggtgccgagc
     2521 cgcgccgtca cgttccagtc gatggtgcga atctcgtcac ccggttgata ttcgcgcaca
     2581 tcctcaaaat tcatcccgcg tcctttgaac acgctgtggt aatctccggc gaaagccgtt
     2641 tcgactaacc cgcgcgttct gatctcgagc gtgcggattt tctttaaaat ttcagcggtc
     2701 ggttccatca gttttgggtt tgcccgctgt agatcgtgga tggttgatgg tcgatggtga
     2761 tttacaaaac tcgtctgcgc actcaatcat tcgggcaagc attgccccga ttttctgcca
     2821 acactgatca tgctccgaaa attcggtctg gttcagatat tcacactgca atgcgtgatc
     2881 gagccatgac tgcgtctcca tcgcttcacc aagagcttga ttaatcttgt cgacaaaggc
     2941 cgggcgatac cttcgtcgag cccacgcctc agcgagcatc gcgtttactg cacgcgaact
     3001 tcgtcgaatc tgatcggtga gcgaatactg ttcagttcgc ggaaagcgtt tactcagttg
     3061 gaatattgca gcagcagcgt tttgcgcggc ctgataaaca ttcaaatctc gaaacgactt
     3121 tgccagcgcc atagatatca ccatctacaa tccacgattc acgatccacg gctaatcacg
     3181 gcaccggcaa cccggcgaaa acccgctcga tgattgtctc actcgtcact gcttcagcct
     3241 ccgcttcgta gctgatgata atgcggtggc gcaaaacatc ggggccgatg cttttcacgt
     3301 cctggggcgt gacgtaaccg cgaccgttaa gaaatgcgtg ggcgcgcgcg ccgagcgcga
     3361 ggtagatggt ggcgcgcggt gatgcaccgt agcgaatcaa gccttcgagc cgcaagttgt
     3421 acgcggctgg atcgcgcgtc gcccagacaa catcgacaat gtaatctttc acgcgatcat
     3481 cgatatagat ctggtttacg acgtcgcgcg cggcgatgat ctgttgcggc tcgaccacgg
     3541 ctcggacgct caggttcggt gccgaggtcg ccatcaaatc aagaatggtg cgctcctctt
     3601 tcttttgagg gtagccaatg tttagcttga acatgaagcg gtcgagctgc gcttcaggaa
     3661 gttgatacgt gccttcctgc tcgatcgggt tttcagtcgc gagcacgagg aacgggtcag
     3721 agagcggata agtttgctcg ccaatcgtaa cctgacgttc ctgcatcgct tcgagcagcg
     3781 cgctctgcac ttttgccggt gcgcgattga tttcgtcggc gaggatcagg tttgagaaaa
     3841 tcggcccgag cttcgtcgtg aactcgcccg tacgcgggtt atagatgagc gtgccgatca
     3901 agtcggccgg cagaaggtcc ggcgtgaatt gaagccgctg aaagcgcgtt tggatgcacg
     3961 cggccatggt cttgacggtg agcgtcttcg cgagaccggg tacgccttcg agtaaaatgt
     4021 gtccgttcgc aagtaggcct aacaacagcc ggtccacgag atatttctgc ccaatgacga
     4081 catgcgctac ctgctcgcgc acgggcggaa tccattggcc gcgttgctgg acctcggccg
     4141 tgatctcttg ggtggacttc atttcgtctg ttttgagaca gatgcgctcg gacaaacgtt
     4201 cgatcaaacg aattacgcgg cgaagatcga catctacttc gcgcgcgttt gttcgcggcg
     4261 gcgcagtgtc aaactgatcg gcaggccaac gtatcgttgt gattggcgaa tgcgcgcttc
     4321 caagtagcgc cggtaaggct cggttagcaa tcgcggatcg ttcacaaaca ggacgaactc
     4381 cggcggcggc aattgtcgat cttgcttcgc gctcgcttgg gccgcgtaaa acaatttgag
     4441 tcgcttgccg gatttctgcg gcggcgcgtt ggcggcaaac gcggtgcgca accgacggtt
     4501 cagtacgccg gtgccgatgt gcgcacgtga ttcgcgctga atcttcgcga ccaggcgaaa
     4561 aagtctgtcg atattttcgc ccgtctgtgc cgatgccacc aacacaggcg cgtaatcgat
     4621 gaagaaaagc cgtgcgcgcg tttcgtcaac gagttgtgtg atggtgcgct tttcgccgcg
     4681 ctttggtttg aataagtccc atttgtttag cacgatgatc gccggtgtcc gcgcttcctg
     4741 aatgagacca gcgattcgtt tgtcctgcgc ggtcacgccg gttgtaagat cgacaatcaa
     4801 gacgcagagg tcagcgcggc gaatgctccg ctccgcgcgc atgacgctga aaacttcgac
     4861 tgagctggaa tgtttgccgc gccgccggat tccagcagtg tcgataaaga gaagttgctc
     4921 accattgcgt tcgtaggaga tgtcgaccgc gtcccgggtt gtgccagcga gttcgctcac
     4981 aattgcgcgc tcgctctgca cgatagcgtt gatgaacgag gacttgccga cattcggccg
     5041 gccaatcacc gcgatggcga gcggaggttt ggtggcagcc ggactggcat ctgatgtttg
     5101 atgtttgatt tcacgggccg gcaataactt gtcgatcttc tccagcagat cggagatacc
     5161 gcggctgtgc tcggcgctga cggatacagt gtggtcgaag cctaacgagt cgaaatccgc
     5221 ggccagcgcg tcatgctttg gattgtcgat cttattgatg acgagaacca ctggttttcg
     5281 cgtcctgcgc agcgagcgcg ccagttctgc atcgacaggg gagacgcctt gctgtccgtc
     5341 gacgacgaac aggattagat cgcttccgcg caaggcctcc tgagcagcgc gctggacttg
     5401 agccgtcagt tcggtttcgc cagcgccgat gatcccgccg gtatccaaga gttggaatgg
     5461 ttgcgcgccg cgggtgcaaa gcgcgtcgag gcggtcgcgt gtgatacctg gttggtcatg
     5521 gacgatggcg attttgcggc cgacgagacg gttaaagaga gcggacttgc cgacgttcgg
     5581 ctggccgatg atggcgacgc tcggcattga tggagtgccg ttcatcgcgg cctaacgaat
     5641 tcagtcgacc cgcgcgttcg cgtggccttc ggcttgtaac tggcgaacac cgtcaagaac
     5701 gtagacgact ccggaaatga gcgtgaagag accggcggag atgacaaccg ccagcagagg
     5761 gatgaaacgg agttgcagca atacccaggc gatcgcgatc atttgcaaca ccgttgctac
     5821 cttgccggtc caattcggtt tcacctggac tttgccgttg agcaggtaaa gcacaattgc
     5881 cccgacgagg atgactgcgt ctcgcgtaat gacgagcacc ggaaaccaga ccggaaatct
     5941 gccgtactcg ggatcggtct gactccaatt actgatactc agggtgatga taccgctcag
     6001 gagcaggccc ttgtccgcga tcgggtcgag gatcacgcct aacgagctgc gctgattgta
     6061 gcggcgggcg atgtagccat ccaggccgtc gctggccgag gcgagcagaa aaatcatgat
     6121 tgcagcgaac cgttgccatt ccaagggcgc gccacgctgg atgctctcgc cgtagtaaat
     6181 ggccatggtg acgaaagccg ggatcatcag aatccgcagc acggtgattt tgttcgccgt
     6241 cgtcatggcg caattactgt agcggcgctc tccgagcgtc gacagttgtt ttgcgagcgt
     6301 gttcggcggt cagagaccgc cgctacagga aagcgtcgcg tcaggtttcc aaagtcacgg
     6361 cggctttggc gggctgctgc tgccctctcg gcatgtgcct accctcggca aattcctcga
     6421 tcatcttgcg gttgaatgca gggatgtcat ccggtttacg gctggtcacc aagccgttgt
     6481 cgaccacaac ttcttcgttc acccaatttc cgcccgcgtt gacgacgtca gtcttaatag
     6541 ccggccaaga tgtgagggtg cggccgcgta ccacgccagc gtcgattagc acccatgggc
     6601 cgtgacagat cgccgcaacc ggtttgccct ggttgaagaa atgccgaaca aactcgagcg
     6661 cctccagtgt tgagcgcagt tggtccggat tcatcaagcc gccgggaatc atgagcgcgt
     6721 cgaactcctc cgggctcgct tcttcgagtg taagatcgac gtcgaacttg tcgcccttat
     6781 cggcatgatg catgccctgg atctgaccgg attccggtga aacgattttc gtttctgcgc
     6841 ccgcattctg gagcgcttcg cgcggtttgg tcatctcgac ctgctcaaac ccgtcagcga
     6901 caaggatcgc cacctttttg cctgctagtg ttttatccat tggttcaaac cagttcagtt
     6961 gtgagaacgc ttacaccgcc gcgagcggat gtataaacca cgtgcgggca gatcatcgcg
     7021 atgtcgaagc gggtgacaaa agtaaaccga tctgccgacg gtatgctggt tttgccttgg
     7081 cggcggcttc ggcaaatcgc cgggcctctt cgctttggcc agtctgactc gcgaaggcgg
     7141 cgcagagcca ttgccggtcg gccgcatcgc ga
//
LOCUS       DASTDG010000201         8551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_458050, whole genome shotgun sequence.
ACCESSION   DASTDG010000201 DASTDG010000000
VERSION     DASTDG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458050"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFO30_06440"
     CDS             <1..709
                     /locus_tag="VFO30_06440"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9418962.1"
                     /translation="TPKDFLQCLTLEHARSLLHRGENVLNVALTAGLSGPGRLHDLCV
                     TLEAASPGEMKTGGADLTIDYGFAMTPFGEALIGETARGICHLSFLNGDGRDGARRLL
                     VDQWPAAKLQRNDKRAGKLAAQIFARRETETRPRLRAFVRGTPFQLRVWRALLQVPPG
                     ALTSYGRISAAINQPIAARAVGSAVGVNPIAFLIPCHRVIRETGVLGNYRWDPVRKRA
                     ILAWELAAHPALNAYAA"
     gene            734..1408
                     /gene="pdxH"
                     /locus_tag="VFO30_06445"
     CDS             734..1408
                     /gene="pdxH"
                     /locus_tag="VFO30_06445"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9418963.1"
                     /translation="MANIVRVMLETPRDPVASHYEHAAQGLRRRDLDPDPIKQFSNWF
                     TAAIEAGIRDVNAMSLATAGRDGKPSVRIVLLKGFDHDGFIFFTNYHSEKGRHLEENS
                     YAACAFYWIELDRQVRVSGAARKISHEESERYFYSRPRGSQLGAWASRQSEVLDARRV
                     LDARMADMTERYGDGPVPRPPHWGGYIVKPDQFEFWQGRANRLHDRFRYTLQADGSWQ
                     VDRLAP"
     gene            1410..1946
                     /locus_tag="VFO30_06450"
     CDS             1410..1946
                     /locus_tag="VFO30_06450"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET9418964.1"
                     /translation="MKTACDKGGPEHGRRINIIRGDITRIDLDAIVNAANTTLLGGGG
                     VDGAIHRAAGPQLLEECKALGGCAPGEAKITRGYNLPARFVIHTVGPYWTGGKRGEAE
                     VLANCYRNSLRLAVEHGIKSIAFPAISCGAYRYPIGQATKIAVETTGAFLAGTDKVEK
                     VVFVLASGEIVGAYRQLL"
     gene            complement(2000..2308)
                     /locus_tag="VFO30_06455"
     CDS             complement(2000..2308)
                     /locus_tag="VFO30_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418965.1"
                     /translation="MLDQAKEEAYWRERHATQPYAKREYSYDDYAAAYRTGYEGFHKY
                     PGKAYEEIEDELALDYERNKAGSALPWDHARHAVHAAWAKVGQDIGPRDADRGIRSGF
                     "
     gene            2416..2901
                     /locus_tag="VFO30_06460"
     CDS             2416..2901
                     /locus_tag="VFO30_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418966.1"
                     /translation="MINPDDDFLEEEPLSAAPEISSEEQTAATVRRRVRRTAPAAAAP
                     TSDSWARVKARTASARERTEVFLRENPVPTILGALGIGLAIGLAIRYASAAREEEKTP
                     ARINWSVLSLPFLWPFLRSVKDKYDESTDALKEGVDRVRNIDMQRYTKPIRKRWKSWA
                     R"
     gene            3041..5011
                     /locus_tag="VFO30_06465"
     CDS             3041..5011
                     /locus_tag="VFO30_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418967.1"
                     /translation="MAKSTASEQPLWQRNPVFEFFASLKLAVVLLAVLILAAIAGTLY
                     ESSFDAKVARTYVYGAPWFNAWLVLLALNLTASALSRWPWRKHHTAFLVTHLGIITLL
                     AGSLIGRIFGVEGTITLFKTDPPTNRLLVDERQLRVHDLDRIIKGFKAEFLHHPPTPK
                     RPRDLGTLASGARLQIIDYAPAIEAKLNPKPLKEGGVPALHFTISTAMMNQNLESWLL
                     ADDAEHSKFSMGLANIELKRSGTSPVEAAVSSGKTEVDLEESIFAFSKAPDEQVGRVS
                     KGGSTGAKVRLEQPQNGNKGRVVVSLGEKEMTFDVAENLGREVKIADSPFTLKIDNYW
                     SDFRIENGKPDSVTDQPNNPAVLVTLRGKGVPAAPQTTDPHGGTNLPATSGPPTMPPA
                     GEEVPDHLTLFIAHDGAISYELVSRNSGKSSGKININQPLATGWADWQLKVDNVLPHA
                     EQSMDFTPVKADSTATATKDLPDAVRVRVEQNGNAIEQWVPAGWQVTVPTSPNETMIA
                     YGWKTFPLPIGLELLDFEVKRNEGSDSPAGFKSTLRVTNSDGGNAIGSCWMNHPFSYP
                     GNALHTWTGLTYKVSQASWNPENLGQSTVQILRDPGWLLKWIGSLLVVVGVFMMFYLQ
                     PYRKQTEGEPIAPPTTGQPKQHRETARVLQHI"
     gene            complement(5065..7542)
                     /gene="lon"
                     /locus_tag="VFO30_06470"
     CDS             complement(5065..7542)
                     /gene="lon"
                     /locus_tag="VFO30_06470"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9418968.1"
                     /translation="MSDVEQISGLQSDPGAKPDTQSTIAAASANQTRIAQIPDTLSIL
                     PVRGFVVFPGTIVPLNVRRTASIRLLDETLLETKIIGLLTQRDEAIEDPTPQDLYHVG
                     TAVLVLKMIRQGDDHVLVIAQGLHRFALRKIVSTTPFLRAEIDLLSSIAPPQSKEWKA
                     TFRNLRDSAAKLFELTPETPEQARLMVLNINNSEQLADFLAPNLNIEVAQKQALLEEL
                     DVEKRLRAVQKYISAQLEIAQIQEKLQKDVASQFSDAQRRAYLRSQIKAIQHELGEGE
                     AGSEEQMQQLRTRLEEAKPPVEVMAQAERELKRLDFIPPASPEYSVIVSYVEIIADLP
                     WAKLSEDNLDLDQAQQTLDRDHYDLEKVKKRLIEYLAVRKLNPQGHGPILCFLGPPGV
                     GKTSLGQSIADALGRKFVRVSLGGIRDEAEIRGHRRTYIGSMPGRIIQELRRAGTRNP
                     VFMLDEIDKIGADFRGDPASALLEVLDPQQNHTFVDHYLDVPFDLSQVIFIATANYID
                     GVPETMRDRMEVISLPGYTEREKLEIAKKYLVRRQMHENGLKPEQCDWREEALRRVIN
                     DYTHEAGVRELERQIGTICRGIAAQVARGKTDHVTITPELVTEMLGPAKYVRETKLKT
                     SKPGVVTGLAYTPAGGEVLHIEATRYPGKGNVTLTGHIGEVMKESAQAALSLVRSRDG
                     AMGVRPNEFRDTDVHVHVPAGAVPKDGPSAGIAMFTALASLFSNTPVRPDVAMTGEIT
                     LRGLVLPIGGLKEKSLAALRAGISTVIIPKLNEKDLVDIPEEAKQQLKFVPVENVDEV
                     LEVALEKNGQKPATRTGAPIPAPQSSP"
     gene            complement(7539..7946)
                     /locus_tag="VFO30_06475"
     CDS             complement(7539..7946)
                     /locus_tag="VFO30_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9418969.1"
                     /translation="MDPINSIKLRWLHGALHDVTYELTRSHFSQFAPHTWEPAINAYR
                     CRSNVRICVDLAGVERSQIDLTVEARRVLIRGARELPEPSHGEGRAVQLLAMEIDYGP
                     FEREVALPVEVEIDKAEAEQRNGLLWISLPVKQ"
     gene            8330..>8551
                     /locus_tag="VFO30_06480"
     CDS             8330..>8551
                     /locus_tag="VFO30_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418970.1"
                     /translation="MQRSAYLLTGAFAIPALRAHAESPPNAQSVLAEVGKVVSSNGID
                     EAKAVEIGGIQQWITVRGRDRRNPLLLVLH"
BASE COUNT         1692 a         2596 c         2486 g         1777 t
ORIGIN      
        1 tacgcccaaa gattttttgc agtgtcttac actcgagcac gccaggagtc ttttacacag
       61 aggggagaac gtcctcaacg ttgctctcac tgccggattg tccggtccgg gccgtctcca
      121 cgatctttgc gtcacgcttg aggccgcttc gccgggggaa atgaaaaccg gcggcgcaga
      181 cctgacgatt gactatggat tcgcaatgac accgttcggc gaagcgctga tcggcgagac
      241 ggcgcgcggc atttgtcatc tgtcttttct caacggggat ggccgcgacg gtgcgcgccg
      301 cctgcttgtt gaccaatggc cggcagcaaa gctgcaacgc aatgacaagc gagccggaaa
      361 attagccgcg caaattttcg cgcggcggga aaccgaaacg cgtccgcgac tgcgggcgtt
      421 cgtgcgcgga actccgttcc agttgcgggt gtggcgcgcg ttgttgcaag tgccgccagg
      481 tgcgctcacg agctatggcc ggatctctgc ggcgattaat cagccgatcg cggcgcgcgc
      541 cgtcggcagc gctgtcggcg taaatccgat cgcgtttctc attccctgcc atcgcgtcat
      601 ccgcgagacc ggagttctcg gcaattatcg ctgggacccg gttcgcaagc gcgcgattct
      661 cgcctgggaa ttggcggcgc atccggcact caacgcctac gctgcgtaac ggcgcgacat
      721 cgacaatcgc cgagtggcga atatcgtgcg cgtcatgttg gagacgccgc gcgatccagt
      781 cgcttcgcac tatgagcacg cagcgcaggg acttcgccgc cgcgatctcg atccggaccc
      841 aatcaagcaa ttttcgaact ggtttactgc ggcaatcgaa gccggcattc gcgatgtaaa
      901 cgcgatgagt ctcgcgaccg ctgggcgcga tggcaaacct tccgtccgca tcgtattgct
      961 gaagggattc gatcatgacg gtttcatctt tttcaccaac taccacagtg agaagggacg
     1021 ccacttggaa gagaactcgt acgcggcatg cgcgttttat tggatcgagt tggatcgaca
     1081 agttcgggtc agcggcgccg cgcgaaaaat ttcacacgaa gaatcggagc gctatttcta
     1141 ttcgcgtccg cgtggcagcc aattgggcgc gtgggcctcg cgccagagcg aagtgctcga
     1201 tgcgcggcgc gtgctcgacg cgcgcatggc cgacatgacc gagcgctatg gcgatgggcc
     1261 ggtgccgcgc ccgccgcatt ggggtggtta tatcgtcaag ccggaccagt ttgaattttg
     1321 gcagggccgc gccaatcgct tgcacgaccg cttccgctac acgcttcagg ccgatggctc
     1381 ttggcaggtc gatcggcttg cgccctgacg tgaaaaccgc ctgcgacaag ggtggtcctg
     1441 agcacggtcg aaggatcaac atcattcgcg gtgacattac gagaatcgac ctcgatgcaa
     1501 tcgtgaacgc ggcaaataca actctgctgg gcggcggcgg agttgatggg gcgattcatc
     1561 gcgcagctgg cccacagctg ctggaggaat gcaaagcgct cggcggctgt gcgcccggcg
     1621 aagcaaaaat cacccgcggc tataatttac cggcgcgctt tgtcattcac accgtcggcc
     1681 cgtactggac tggcggaaaa cgcggcgaag cggaggtgct ggccaactgc tatcgcaatt
     1741 cactccggct cgcggtcgag cacggaatca aatcaatcgc ttttccagcc atcagttgcg
     1801 gggcatatcg ctacccgatc gggcaagcga caaagatcgc cgttgagaca acgggcgctt
     1861 tcctggcggg caccgataag gtcgagaagg tagtgttcgt gttagcgagc ggcgaaatcg
     1921 tgggtgcgta tcggcagtta ctgtagccac ggccccgtgg gccgttattg gacgacgcgc
     1981 cacaggcgtg gctacaagct taaaatccgc tgcgaatccc ccggtcggcg tcgcgtggac
     2041 cgatgtcctg gccgaccttc gcccacgcgg cgtgcacagc gtggcgcgca tggtcccacg
     2101 gcagagccga gccggctttg ttgcgctcgt agtcgagcgc cagctcgtcc tcgatttcct
     2161 cgtaagcctt gcccggatac ttgtgaaaac cttcgtatcc ggtccgatat gccgccgcgt
     2221 aatcgtcata agaatactct cgcttcgcgt acggctgcgt ggcatgacgc tcgcgccagt
     2281 acgcttcttc cttcgcctgg tccagcatgt tgctcctttc gctactccat caaactttcg
     2341 cggcctgaaa cggccttgga cgtggaaaga gccgagaaac gcaatcaccc gccgagttgc
     2401 gattcagtaa ttgctatgat caatccagac gacgattttc tcgaggagga gccgctctcg
     2461 gcggcgccag aaatcagcag tgaagagcaa accgctgcaa ctgtccgtcg gcgagtgcgt
     2521 cgcacggccc ccgcagccgc agcgccgacg agcgatagtt gggcgcgggt gaaggcccga
     2581 accgcgagcg cgcgtgaacg gacggaagtt ttcctgcgcg aaaatccagt gccgacaatc
     2641 ctcggcgcac tcggcattgg cctcgccatc ggactggcca tccgctatgc gtcggcggcg
     2701 cgcgaagagg agaaaactcc agcccggata aactggagcg ttctctcgct gccatttttg
     2761 tggccgttcc tgaggtcggt caaagacaaa tacgacgagt cgaccgacgc gctaaaagaa
     2821 ggcgtggatc gcgtccgcaa catcgacatg cagcgctaca ccaaaccgat ccgcaaacgc
     2881 tggaaatctt gggcgcggtg aatgtatgtc gcgcttaggt ggatcgagct ctgcgagctc
     2941 gatgctaaat taaaggacct gcggcgccat accatcgtct gcggagcgct cgatctacct
     3001 tgaagcggca attgtcctcg cccaagatgt caatctccgc atggcgaaat cgaccgcttc
     3061 cgagcagccg ctttggcaac gcaacccggt cttcgagttt tttgcttcgc tcaagctcgc
     3121 cgtcgttctg ctcgccgtcc tgatcctcgc cgccatcgca gggacgcttt acgaatcgag
     3181 cttcgacgcg aaagtcgctc ggacctacgt atacggcgcg ccctggttta acgcctggct
     3241 cgttctgctc gcgctcaatt tgaccgcctc ggctctgtcg cggtggccgt ggagaaagca
     3301 tcacaccgcg tttctcgtca cgcatcttgg catcattact ttgctcgccg gctctttgat
     3361 cgggcgcatc ttcggagttg aaggcacgat taccctcttc aaaaccgatc cgccaacgaa
     3421 ccggctcctt gtcgatgaac gccaactccg cgtccacgac ctggaccgaa tcatcaaagg
     3481 tttcaaagcc gagtttctgc accatccacc taccccaaaa cggccgcgcg acctcggcac
     3541 gctggcgagc ggcgcgcgct tgcagatcat cgattacgcg cccgcgatcg aggcgaaact
     3601 gaacccgaag ccgctcaagg aaggcggcgt gcccgcactg cacttcacta tttccacggc
     3661 gatgatgaac caaaacctcg agagctggtt gctcgccgat gacgccgagc actccaaatt
     3721 ctcaatgggt ctcgccaaca tcgagctcaa gcgcagcggc acgtcacctg tagaggccgc
     3781 tgtctctagc ggcaaaacgg aggtcgatct tgaggaatcg attttcgcat tcagcaaagc
     3841 gccggacgaa caggtcgggc gcgtgagtaa aggcggcagc accggcgcca aagtgcgact
     3901 cgagcaaccg caaaacggaa ataagggacg cgtcgttgtt tcgttaggcg aaaaggagat
     3961 gaccttcgac gttgccgaaa atctcggacg cgaagtgaag atcgccgatt cgcctttcac
     4021 gctgaagatc gacaattact ggtccgattt ccgaatcgag aacggcaagc ctgattcggt
     4081 caccgatcag ccgaacaatc cagctgtgct cgtgacgctt cgtggcaaag gcgttccggc
     4141 tgctccgcaa acgacagatc cgcatggtgg aaccaacctg ccggcaacta gcggcccgcc
     4201 cacgatgccg ccagcgggcg aagaagtgcc cgaccatctc acgctgttca tcgctcacga
     4261 cggagcaatc agctacgagc tcgtttcgcg gaactcgggg aaatcgagtg gtaagatcaa
     4321 catcaaccag cccctcgcca ccggctgggc agattggcag ttgaaggtcg acaatgtgct
     4381 gccgcacgcg gagcaatcga tggatttcac cccggtgaaa gcggacagca cagcgacggc
     4441 cacgaaggat ttaccagatg ccgtgcgcgt tcgcgtcgag cagaacggca atgcgatcga
     4501 gcaatgggtg ccggccggct ggcaggtcac ggtcccgacc tcgcctaacg aaacgatgat
     4561 cgcctacggt tggaagacgt ttccattgcc gatcggactc gaactgctcg atttcgaagt
     4621 gaaacgcaac gaaggcagcg atagtcccgc tggattcaaa agcacattgc gagtgaccaa
     4681 ttcggacgga ggaaacgcga tcggttcctg ctggatgaat catccattca gttaccccgg
     4741 taacgcgctg cacacgtgga ccggattgac ttacaaggtc tcgcaggcgt cctggaaccc
     4801 ggagaacctc ggtcaaagca cggttcagat tttgcgtgac cccggttggc tgctgaaatg
     4861 gattggatcg ctgctcgtcg tcgtgggcgt gttcatgatg ttttatctcc agccctaccg
     4921 caaacaaacc gaaggcgagc cgatcgcgcc gccgaccaca ggccagccca aacagcaccg
     4981 cgaaactgca cgagtcttac agcacatctg aagctataag atcttggatg caccctcacc
     5041 tggcgctgtc gcgtcctcct ctcctcaggg agaggactga ggcgcgggaa tcggtgcacc
     5101 agtgcgtgtg gccggttttt ggccgttttt ctccagcgcc acctccagaa cttcgtcaac
     5161 gttttccacc ggcacaaact tcagttgttg cttcgcttcc tccggaatgt cgacaagatc
     5221 cttctcgttc agcttcggga tgatgacggt ggaaatgccg gcgcgtagtg cggctagact
     5281 tttttctttc aggccgccga tcggcagaac gagtccgcgc agcgtgattt ccccggtcat
     5341 cgcgacatcc ggacggaccg gtgtgttgct gaagagcgac gctagcgcgg tgaacatcgc
     5401 aattccagcg gaaggtccgt ccttgggcac tgcgcccgcg ggtacatgca catgcacatc
     5461 ggtatcgcgg aactcgttcg gtctcacgcc catggcgcca tcgcggctgc ggacaaggct
     5521 gagcgcggcc tgcgccgatt ctttcatcac ctcgccgatg tgacccgtaa gcgtgacatt
     5581 gcctttcccc ggatagcgtg tcgcttcgat gtgcaaaact tcgccgccgg ccggcgtgta
     5641 cgcgagacca gtcacgacgc ccggtttgct cgttttcagt ttggtttcac ggacgtattt
     5701 ggctgggcct aacatctcgg tgaccagctc cggcgtaatc gtcacgtgat cggttttccc
     5761 acgggcgacc tgtgccgcaa tcccgcggca gatcgtgccg atctgccgct caagttcgcg
     5821 cacaccggct tcgtgtgtgt aatcgttgat gacgcgccgc agtgcttcct cccgccaatc
     5881 gcattgctcg ggcttgagcc cgttctcgtg catctgccgc ctaacgagat atttcttcgc
     5941 aatctccagc ttctcgcgct ccgtgtagcc gggcagagag atcacctcca tgcggtcccg
     6001 catcgtctcg ggcacgccgt cgatatagtt cgccgtcgcg ataaagatca cctgcgacaa
     6061 atcgaatgga acatcgagat aatgatcgac aaaggtatgg ttctgctgcg gatcgagcac
     6121 ttcgagcagc gcgctcgccg ggtcgccgcg gaaatcggcg ccgatcttat caatctcatc
     6181 gagcatgaaa accgggttgc gcgtcccggc gcggcgcagc tcctgaataa ttcgccccgg
     6241 cattgagccg atgtaggtgc ggcgatgacc gcggatttcg gcttcgtcgc gaatgccgcc
     6301 gagactgacg cgcacaaatt tacgcccaag tgcatcggca atggactggc cgaggctggt
     6361 tttaccgacg ccgggcggtc cgagaaaaca caagatcgga ccatggccct gcgggttgag
     6421 tttgcgcaca gcgaggtact cgatgagccg cttcttcact ttctcaaggt cgtagtggtc
     6481 gcgatcgagt gtctgctgcg cttgatcaag atcgagattg tcctcgctca attttgccca
     6541 cggcagatcg gcgatgattt cgacgtagct aacaatgacg gaatactccg ggctggccgg
     6601 tggaataaaa tcgagccgtt tcaactcgcg ctcggcttgc gccatcactt ccaccggggg
     6661 cttcgcttcc tcgagtcgcg tgcggagttg ctgcatttgt tcttcgctgc cggcttcgcc
     6721 ttcgcccagc tcatgctgga tcgctttgat ttgcgagcgc agataggcgc ggcgttgcgc
     6781 atccgagaac tgcgacgcca cgtccttctg cagcttctcc tggatctgcg cgatctcgag
     6841 ctgcgctgag atatattttt gcaccgcgcg gaggcgcttc tcgacatcga gttcttcgag
     6901 cagcgcctgt ttttgcgcaa cttcgatgtt caagttcggc gcgagaaaat cggcgagctg
     6961 ctcggaattg ttgatgttga gcaccatcaa tcgcgcctgc tcgggggttt ccggtgtaag
     7021 ctcgaaaagc ttcgccgccg agtcgcgcag gttgcgaaag gtcgccttcc attccttgct
     7081 ctgtggcggc gcgatcgagg agagcagatc gatctcggcg cgcaaaaacg gcgtcgtgct
     7141 gacgattttg cgcagggcaa accgatgcag accctgcgca ataaccagca cgtggtcgtc
     7201 cccttgccga atcatcttca acacgagcac agccgtgccg acgtgataca gatcctgcgg
     7261 cgtcggatct tcgatcgctt cgtcgcgttg cgtcagtaac ccgatgatct tcgtttccag
     7321 cagtgtttcg tcgagcaatc gaatcgacgc cgtgcggcgc acgtttagcg gcacgatcgt
     7381 gccggggaaa acgacgaagc cacgcactgg caagatcgac agtgtgtcgg gaatctgcgc
     7441 gatccgcgtc tgattggcgc ttgccgccgc gatcgtgctt tgcgtgtcgg gttttgcgcc
     7501 cgggtcactt tgcaacccgc tgatttgttc gacgtcgctc actgtttcac cggcagcgag
     7561 atccaaagta aaccattgcg ctgctcggcc tctgctttgt caatctccac ctccacgggc
     7621 aatgcaactt cgcgctcgaa cggcccgtaa tcgatttcca tcgccagcag ctgcaccgcg
     7681 cggccttccc catgcgacgg ctcgggcaac tcacgcgcgc cgcgaatgag cacgcggcgc
     7741 gcttccacgg tcaggtcgat ctgtgagcgt tcgactccgg caagatcgac acagatgcgc
     7801 acgtttgacc ggcagcgata cgcgttgatc gccggctccc acgtgtgcgg cgcaaactgc
     7861 gaaaaatgcg aacgcgtcag ctcataggtg acatcgtgaa gcgcgccgtg cagccagcgt
     7921 aacttaatgc tgttgatcgg gtccatgtga caagtctaac tcacgcggcg cgtctgcgcc
     7981 cgttccaacc gctttatttc accggaatcc ttttccggcg tttcgcctcc acgcccggaa
     8041 gttctgcgca caccttcacc tctttgtccg tttcggttac gttcgctccc gaaatggtca
     8101 aacaaacggt tgatctggtt gcgcaaatcc atgagcgggt cgccgccccg cgtcgcgggt
     8161 gcgcgttcat tactccttct cgatggaatg attgccataa aacaggcctc ctccctggtt
     8221 ttcgtgctct gtactagtta cgcggagcac accatcttcg cgtgtttgcc tgagatcgat
     8281 tgacgatgtc gatcttgcct aacatcagag aggattgaat ttcatctgga tgcagcgttc
     8341 tgcgtatctt ctcaccggcg cgttcgcgat ccccgcgctt cgcgcgcacg cagagtcgcc
     8401 gccgaatgct caaagcgttc tcgcggaagt aggcaaggtc gtttcctcaa acggaatcga
     8461 tgaagcaaaa gcggtagaga tcggcggcat tcagcaatgg atcacggtgc gcggacgcga
     8521 ccggcgtaac ccactcctgc ttgtgctgca t
//
LOCUS       DASTDG010000202         6212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_795862, whole genome shotgun sequence.
ACCESSION   DASTDG010000202 DASTDG010000000
VERSION     DASTDG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795862"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFO30_06485"
     CDS             <1..591
                     /locus_tag="VFO30_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9418971.1"
                     /translation="IGNLGLNPAQCKRPSVIIIGATVTLYPEAVYQTGLNVVTCATRR
                     TNPSALNPAVKSLNYLNNVMARIEANLAGADEALMLNDAGNVAECTADNVFIIKRGQI
                     FTPPITAGALRGITRAVVFEIAAELGIKVTETDITRHDVFIADECFLTGTAAEIIPVI
                     EADGRPIGTGKPGPICARMMARFRQLTRETGMPIYS"
     gene            680..1177
                     /locus_tag="VFO30_06490"
     CDS             680..1177
                     /locus_tag="VFO30_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET9418972.1"
                     /translation="MLCDVCKCNDATVFLTQILEGKMQKVNLCEACSKEKGVQDPTGF
                     ALADLLLGIGAAEEIEKGAPSQKCPVCGFTQADFKKTGRLGCSACYVTFAEALNSLLK
                     AMHKGTEHVGKLPERAQRTLELADRVRSLTKSLEKAVAEENYETAASLRDQIKQLEHE
                     LTSPT"
     gene            1174..2286
                     /locus_tag="VFO30_06495"
     CDS             1174..2286
                     /locus_tag="VFO30_06495"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HET9418973.1"
                     /translation="MKFDSVISTAGEWLRGEGPHHQVVISSRVRLARNLRDRAFPGWA
                     KKAERVAILDLIKPRVEELGEMQDSFSESLHDLSALEKQVLVERHLISREHAAKGAGS
                     AVVMNRRQTVSIMINEEDHLRMQSIRSGLQLRQAFKLIDKIDSALEGKLHFAYDQRLG
                     YLTACPTNVGTGLRASAMLHLPGLVLSDLINQVVQAVSKIGLAVRGLYGEGTEAMGNL
                     FQISNQTTLGEKEDEIIGRLTKVIETIIDKEQDARQVLIQKKSNTLWDQVGRAYGVLN
                     YAHAITSKEALNLLSILKLGVDLGAFPEDRRFAIDQLFMDTQPAHLQKGSQQKLNAEE
                     RDHYRAEIVRERLKVFPKPDIGKMARGSGNGSVAEN"
     gene            2323..4797
                     /locus_tag="VFO30_06500"
     CDS             2323..4797
                     /locus_tag="VFO30_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9418974.1"
                     /translation="MNNFTPRAQQVLALARKEADRFNHNYVGTEHLLLGLIKLGQGVA
                     VNVLQKMGLDLETVRMEVEKQVGSGPETKMVGNIPYTPRVKKVLALAGKEAKALNHSY
                     VGTEHILLGLLREGEGVAARVLKSLEVDIERTRNEILKELDPNFMPAEAEQETGEPTS
                     KKDVKTPALRAFGRDLTELARKGELDPVIGRRNEIERVIQVLCRRTKNNPVLIGEAGV
                     GKTAIAEGLAQEIANGNVPELLRDKRVVTLDLALMVAGTKYRGQFEERIKAVMDEIRR
                     SKTVILFIDELHTIVGAGSAEGAMDASNIIKPALSRGELQCVGATTMNEYRKYIEKDA
                     ALERRFQTVKVDAPTIDEAIEILKGLRPKYEAHHKAKLTDEALETAVKFSERYITGRF
                     LPDKAIDVMDEAGARARIAAMTRPPDVKDIEKEIEQIRLEKEAAIKAQDFEKAAALRD
                     KEKQTKEKLDTILGKWREEREEKQVLVTGDDMMHIISRVTGVPLQRMEQKETEKLLRM
                     EEELKGKVIGQDEAVTAISKALRRSRADLKDPRRPIGSFIFLGPTGVGKTFLAQKLAE
                     FMFGDRDALIQIDMSEYMEKFTASRLIGSPPGYVGYEEGGQLSEAVRRRPYSVVLFDE
                     IEKAHPDVMHLLLQILEDGKITDSLGRKIDFRNTIIIMTSNVGAETLRRQTTMGFAAT
                     KPIGEHEYEGMRDRLLEEAKKTFKPEFINRLDDIIVFHQLSKPDLMKIVDLEVDKVLE
                     RVRAKEVHIILDDRAKEFLIEKGYDPMYGARPMRRAVERYLEDPLAEEILRSNVKPGD
                     TVHVSAEHEKLVFKTPERVPTAAPAS"
     gene            complement(4869..5765)
                     /gene="xerA"
                     /locus_tag="VFO30_06505"
     CDS             complement(4869..5765)
                     /gene="xerA"
                     /locus_tag="VFO30_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HET9418975.1"
                     /translation="MAARQETDPLAKEFLNYLAVERNASRRTLKAYRDALRAFRAKKT
                     TPWARCSVEDFRDYLFELMKHEQARSYIRLQFSALRTFYRFLKERKKLKRDPVRELQL
                     PKLEKKLPLVLTRNQVEELLAAPFKVEKNRAAPKWMPLRDVAVMELFYSSGLRLSELA
                     ALDVADVDLYTELVRVFGKGRKERLCPIGAPALEAISRYRSAANVHAGPLFINKGRKR
                     ISSRSIWLSLRRYLRHTSIPISISPHKLRHSFATHLLDRGADLRSVQALLGHASLSTT
                     QIYTHVSVERLKKAYAASHPRA"
     gene            complement(5767..>6212)
                     /locus_tag="VFO30_06510"
     CDS             complement(5767..>6212)
                     /locus_tag="VFO30_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418976.1"
                     /translation="VLNSCQTLPAGIQQARAAMAQSISNEPIGDYYIGRRYFKPDFKF
                     WGYVRRPGQPWSTAQLVMLNEKQKLAPDRERLAFGSDNNYEYKLNGYFSGDKVYEPAS
                     NGFYPEFVLQGYELISTNPPPIFKSQYDSRAQAASNRYLIEKPEP"
BASE COUNT         1475 a         1717 c         1824 g         1196 t
ORIGIN      
        1 attggtaatc tgggcctgaa ccccgcgcaa tgtaagcggc cgagcgtcat cattatcggc
       61 gcgaccgtaa cgctatatcc ggaagcagtt taccaaacag gtctaaacgt ggtgacgtgc
      121 gcgacacggc gcacaaaccc gagcgcgctc aatcccgccg tgaagtcgtt aaactacctc
      181 aacaatgtca tggcgcgtat tgaagcgaac ctcgccggtg cagatgaggc gctcatgttg
      241 aacgacgccg gtaacgtggc ggagtgcacc gccgacaacg tcttcatcat caagcgcggc
      301 cagatcttca caccgccaat cacggccggc gcattgcgtg gcatcactcg cgcagtcgtg
      361 ttcgagatcg cagccgaact gggcatcaaa gtgaccgaaa cagacatcac gcggcacgat
      421 gtgttcatcg ccgacgaatg ttttctcacc ggcacggcag cggaaataat cccggtcatc
      481 gaggcggatg gccggccaat cggcaccggc aaaccgggcc caatctgcgc gcggatgatg
      541 gcccgtttcc ggcagttaac ccgcgaaact ggaatgccca tctatagtta aactcccggt
      601 aatgcgatcg ggcgcggaag gtgctgaacc gaattgcggg gttgaatttt tgacccaaaa
      661 aaaggtctaa ttagcagaaa tgttgtgcga cgtctgcaaa tgtaacgacg caaccgtctt
      721 cctgacgcaa atcttggaag ggaagatgca aaaagtgaat ttgtgcgagg cctgctcgaa
      781 agaaaaaggt gtgcaggacc caaccggctt tgcgctggct gatctgttgc tcggtatcgg
      841 cgcggcagag gagatcgaga aaggcgcccc gagtcagaaa tgtccggtct gtggattcac
      901 gcaagccgat ttcaaaaaga ccgggcgtct cggatgtagc gcatgctacg taacgtttgc
      961 cgaagcgctg aacagtttac tcaaagcgat gcataaaggc accgaacacg tcggcaagtt
     1021 gcccgagcgc gcgcaacgca cgctcgagct ggccgaccgg gtgcgcagcc tgacgaaaag
     1081 tttggaaaaa gcagtcgcgg aggaaaacta cgagacggct gcttctctcc gcgaccaaat
     1141 taaacagctc gagcacgagc tcacgagccc gacatgaaat tcgacagtgt aatatcaaca
     1201 gcgggagaat ggctgcgcgg ggaaggtcca catcaccagg tcgtgattag cagtcgtgtg
     1261 cggctggcgc gtaatctgcg cgaccgcgcg tttcctggat gggcaaaaaa agctgagcga
     1321 gtggcgattc tcgatttgat caagccgcgg gtggaagagc ttggcgaaat gcaggattca
     1381 ttctcggaat cactgcacga tttatccgcg ctggaaaaac aggtgctggt cgagcggcat
     1441 ctgatcagtc gcgaacatgc ggcgaaaggt gccggcagcg cggtcgtgat gaaccgccgg
     1501 caaaccgtca gcatcatgat caacgaagag gatcacctgc gcatgcaatc cattcggtcc
     1561 ggtctgcagc tgcggcaggc gtttaaactt atcgacaaga tcgacagtgc gctggaaggc
     1621 aagttgcatt tcgcctatga ccagcgcctc ggatatttga cagcgtgtcc gacgaacgtc
     1681 ggcaccgggt tgcgcgcgtc agccatgttg cacttacccg gcttggtctt gagcgacctg
     1741 atcaatcagg tcgtgcaggc ggttagcaag atcggcttgg ccgtgcgcgg actttatggc
     1801 gaaggcaccg aagcgatggg caacctgttc cagatttcca atcaaacgac gctcggcgaa
     1861 aaagaagatg agatcatcgg tcgactcacg aaagtcatcg aaacgattat cgacaaggag
     1921 caggacgcgc ggcaggtctt gatccagaaa aaatccaaca cgctgtggga ccaggttggt
     1981 cgcgcctatg gcgtgttgaa ttacgcgcac gccatcacct cgaaggaagc gctcaattta
     2041 ttgtcaattt taaaactcgg cgtcgatctc ggcgcctttc cggaagaccg ccgcttcgcg
     2101 attgatcagc tcttcatgga cacacagccg gcgcatttgc agaaaggttc acagcaaaag
     2161 ctcaatgccg aggaacgcga tcattaccgc gcagaaattg ttcgcgaacg tttgaaagtt
     2221 ttccccaaac ctgatatcgg taaaatggcg cggggttcgg ggaatggctc ggttgccgaa
     2281 aattgaatct gttactattt tgatttagcc gagcgactga cgatgaacaa ctttaccccc
     2341 cgtgcgcaac aggtcctcgc gctcgcgcgc aaggaagctg accgcttcaa tcacaattat
     2401 gtcgggaccg agcatctctt gctcggcttg attaagctgg gccagggggt cgcggtgaac
     2461 gttctgcaaa agatgggcct cgatctcgag acggtgcgga tggaggtcga gaaacaggtt
     2521 ggctccggtc cggaaacgaa aatggtcggc aacatcccct acacaccgcg tgtgaaaaag
     2581 gtcctggcgc tcgccggaaa agaagccaag gcgctcaatc actcttatgt cggcacggag
     2641 cacatcttgt taggcctttt gcgcgaaggc gaaggcgtcg ccgcgcgcgt gctcaaaagc
     2701 ctcgaagtcg atatcgaacg cacgcgcaat gagattctga aagaactcga tccgaacttc
     2761 atgcccgccg aggccgagca ggaaaccggc gagccgacct cgaagaaaga tgtcaaaaca
     2821 ccggcgttgc gcgctttcgg gcgcgacctg accgagttgg cacggaaggg cgagctcgac
     2881 ccggttattg gccgccgcaa cgaaatcgag cgcgtcattc aagttctctg ccggcgcacg
     2941 aagaacaatc cggtgctcat cggcgaagcc ggcgtgggca aaacggccat cgccgaaggt
     3001 ctcgctcagg aaatcgcgaa cggtaacgtg ccggaactgt tgcgcgacaa gcgcgtggtg
     3061 acgctcgacc tggccttgat ggtggcgggg acaaaatatc gcggccagtt cgaagagcgc
     3121 atcaaagcgg tgatggacga aatccgccgc tcaaaaaccg ttatcctctt catcgacgag
     3181 ttgcacacta tcgtcggggc cggatcggcc gaaggcgcga tggacgcctc caacatcatc
     3241 aagcccgcgc tgagccgcgg cgagctgcaa tgtgttgggg caaccacaat gaacgagtac
     3301 cgcaaataca tcgagaagga cgcagccttg gagcgtcgct tccaaacggt gaaggtcgac
     3361 gcgccgacaa tcgatgaggc gattgagatt ttgaaagggt tacgcccaaa atacgaagcg
     3421 caccataaag ccaagctcac cgacgaagcg ctcgagacgg cggtgaaatt ttccgagcgc
     3481 tacatcaccg gccggttcct gccagacaag gcaatcgatg tgatggacga agccggcgcg
     3541 cgcgcccgta tcgcggcaat gacacgtccc cccgacgtga aagacatcga aaaagagatc
     3601 gagcagattc gactcgagaa agaggccgcc atcaaagcgc aggattttga aaaggccgcc
     3661 gcgctccgcg ataaagagaa gcaaaccaag gagaagctcg acacaatttt ggggaagtgg
     3721 cgcgaagaac gcgaagagaa acaggtgctg gtgaccggcg acgacatgat gcacatcatc
     3781 tcgcgcgtca ccggcgtgcc gctacagcgg atggagcaaa aagagacgga aaaacttctc
     3841 cgcatggaag aggagctcaa aggcaaggtc attgggcagg acgaagccgt cacggcgatc
     3901 tcgaaggcac tgcgccggag ccgcgccgat ctcaaagacc cgcgacggcc gattggctcg
     3961 ttcatttttc tgggcccaac cggcgtcgga aaaacattcc tcgctcagaa gctcgccgag
     4021 ttcatgtttg gagatcgcga cgcgctcatt cagatcgaca tgtcggagta catggaaaaa
     4081 ttcaccgcgt ctcgattgat cggttcgccc ccgggttatg tcggttacga ggaaggcggt
     4141 cagctttccg aggccgtccg gcgccggccc tactcggtcg tgctcttcga cgagatcgag
     4201 aaggcgcatc cggacgtgat gcatctgctt ctgcaaatcc tcgaagacgg gaagatcacc
     4261 gattcgttag gccgaaaaat cgacttccgc aacacgatca tcatcatgac ctcgaacgtc
     4321 ggcgctgaga cactgcgccg gcagacgaca atgggtttcg ccgccaccaa gccgatcggg
     4381 gagcacgaat acgaggggat gcgcgaccgc ttgctcgaag aagccaagaa aaccttcaag
     4441 cccgagttca tcaaccgtct cgacgacatc atcgtcttcc atcagttgag caagcccgac
     4501 ctgatgaaga ttgtcgacct tgaggtcgac aaagtgcttg agcgggttcg cgccaaggag
     4561 gtgcacatta tcctcgacga tcgggccaaa gaatttctca ttgagaaggg ttacgatcca
     4621 atgtacggcg cgcgtccgat gcgccgcgcg gttgaacgct acctcgaaga cccactggcc
     4681 gaggaaattc tgaggagcaa cgtgaaaccc ggagatactg ttcacgtgtc cgccgagcac
     4741 gaaaagctag tcttcaaaac accggaaagg gttccgactg cggcgccggc aagctaatct
     4801 gcagaggccg ccgtcctcgg cggcagagtt cgcgccccgc cgctgagaca gaggctacta
     4861 cgttgctatc atgcacgcgg gtgcgaggcg gcgtacgctt tcttcagccg ctcgacgctt
     4921 acgtgggtgt agatttgcgt ggtcgataag ctggcatgac cgagcaaggc ttgcacgctg
     4981 cgtaaatccg cgccgcggtc gagcaagtgg gtcgcgaaac tgtgccgaag tttatgcgga
     5041 ctgatcgaga tcggaatcga tgtgtgccgc agataccgtc gaaggctcag ccaaatcgat
     5101 ctcgatgaaa ttcgcttccg ccctttgttg atgaacagcg gacccgcgtg cacgttcgct
     5161 gctgatctgt agcgcgagat cgcttccagc gccggcgcac caattggaca cagccgttcc
     5221 tttcggcctt tgccgaaaac acgcaccaac tcggtgtaaa gatcgacatc agcaacgtcg
     5281 agcgcggcga gctcgctcaa gcgcagcccg ctgctataga aaagttccat cacggcaaca
     5341 tcgcgtagcg gcatccattt cggggcagcc cggtttttct cgaccttgaa cggcgcggcg
     5401 agcagctctt ccacctgatt acgcgtgagc acgagcggaa gcttcttttc gagctttggc
     5461 agttgcagct cgcggacggg atcgcgtttc aatttctttc gctccttcag gaagcggtaa
     5521 aatgtgcgca gcgcggagaa ttgcagccgg atatacgacc gcgcctgttc gtgcttcatc
     5581 aactcaaaca ggtaatcgcg gaaatcttcc acgctgcatc gcgcccaagg cgtggtcttc
     5641 ttggcgcgaa aagcccggag cgcgtcgcga tatgctttta acgtccgccg tgaagcattc
     5701 cgctcgaccg ccaggtagtt gagaaactct ttggccaggg gatccgtctc ttgtcgcgcg
     5761 gccaagttac ggctccggct tctcgatcaa gtaacggttg ctcgcggctt gcgcgcggct
     5821 gtcgtactgg cttttgaaaa tcgggggcgg atttgtggaa atcagttcgt agccctgtaa
     5881 gacgaactct ggataaaagc cgttactcgc cggttcgtaa actttgtcgc cgctgaaata
     5941 gccgttgagc ttgtattcgt aattgttgtc gctaccgaag gcgagccgtt cgcggtcggg
     6001 cgcgagtttt tgtttctcgt ttagcatcac cagctgcgct gtgctccacg gctgcccggg
     6061 ccggcggacg tatccccaaa atttaaaatc aggtttgaag tagcgccgtc cgatgtagta
     6121 gtcgccgatt ggttcgttcg agatactttg cgccatcgcg gctctggcct gctgaatacc
     6181 tgccggcaac gtttgacagc tgttgagaac ga
//
LOCUS       DASTDG010000203         8057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_77834, whole genome shotgun sequence.
ACCESSION   DASTDG010000203 DASTDG010000000
VERSION     DASTDG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77834"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFO30_06515"
     CDS             complement(<1..975)
                     /locus_tag="VFO30_06515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9418977.1"
                     /translation="MKNHVAMCAAAALIIAPLHGYAQERSTTNQQATPSETATSLDPA
                     AATQAWLASVPPDQRAKSDAYFEGGYWLILWNFLLAAAISIFLLASRISARVRDCAQR
                     LTRFRPAQVALYSFAYLIITCTLSFPLNLYQNFFREHQYGMATQIFAQWFGDQCIGLA
                     LLLVLGTISLVALYAVFQRAPRTWPVWGAIVAVVFGMIGLFIAPIYIEPLFNTYKPLT
                     DPAIKEPILAMARANQIPVTQVFEVDASRQTKRVGANVAGFLGTTRIALNDNLLRQCT
                     LPEIREVMAHEMGHYVLNHVTKLLVDAGIVVLIGLAITRAAFERAVARW"
     gene            1063..1425
                     /locus_tag="VFO30_06520"
     CDS             1063..1425
                     /locus_tag="VFO30_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418978.1"
                     /translation="MNAVKMRVLVVCALLVGGASGALTETRTPDPEWYGPYPANYKEI
                     VTKWLETQLLDPTSARIEWKDEPKPADLGSKGEHLYGYLVTFTVNARNRFGTYTGKQV
                     HGALIRNGEVIKGVGFGY"
     gene            1429..1893
                     /locus_tag="VFO30_06525"
     CDS             1429..1893
                     /locus_tag="VFO30_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418979.1"
                     /translation="MKLPNKLQIREAGQAIGVLVVLLAIIGIGVWLLYSYRNGMRDEG
                     RAFGRQVIERVAVQHDANFLATSLAPQARLNLPPPMQQELMDRLRQLGTPVGQPNAQG
                     DVTFESQFFEPHGAFYAQLNYPAGSMRMDVAISHPVGRWQVDSITLSTVGAR"
     gene            complement(1972..2586)
                     /locus_tag="VFO30_06530"
     CDS             complement(1972..2586)
                     /locus_tag="VFO30_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418980.1"
                     /translation="MSQPNENVLVVRRSLFDQLGSFQGLNFEPEKYLRELLRRGNNFF
                     LPRAEAEHDPAHKQLIPYVLLAFDDKVLHYVRGKKAGEQRLVAKGSIGIGGHMNDRDE
                     SLFALDQAAYRAGVEREVNEEIKIESQFEDHIVALLNDDASEVGLVHLGIVHVFKLAQ
                     PKVEKREAMITNLAFLSKEELLARRDSLETWSQLCVGALDRLLA"
     gene            complement(2602..3210)
                     /locus_tag="VFO30_06535"
     CDS             complement(2602..3210)
                     /locus_tag="VFO30_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET9418981.1"
                     /translation="MKRFVLMALFAFTVGAPRGFAGPAEVSTLAAAQLNPPRFQIALE
                     SGYLFGAINPPQAYQIGAEFLTARVRWGVLERTDWLRGYNQFYISAMAEPIFKGIENH
                     YFGLNFGGRYNFVPPGSRWVPYVSGGLGLGWIDSHANIPGGQGQDFTFNILSAAGLAY
                     TIDDRWSVNAGVLYQHLSNGGQTNPNPSLNLFGPQVGITCSF"
     gene            complement(3290..4042)
                     /locus_tag="VFO30_06540"
     CDS             complement(3290..4042)
                     /locus_tag="VFO30_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HET9418982.1"
                     /translation="MDESEDYVVVDKPPFLLSHPTKPTEQTTLWKELRELLAFEIANG
                     GQVSIVNRLDRETSGLVLVAKTAAAARRFGLLMQFQGFEKEYLAIVWGWPACDSKIVD
                     APLDRQGKHQPTRIWLEQMIHPAGAPARTDFIIEQRFTRPVLSEVAGSTSSGNKFALI
                     RAIPRTGRTHQIRVHLASLGHPIVGDKIYGPDERLYLEFIETGWTPALHRQLLLPRHA
                     LHATRLAIPGEHKWSSSLPLDLAEFIGCSGDL"
     gene            4078..5775
                     /locus_tag="VFO30_06545"
     CDS             4078..5775
                     /locus_tag="VFO30_06545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9418983.1"
                     /translation="MMKRKSLSVLLTFFGCMSISFSAGPEKQAFSADLMIINGVVHTM
                     DSAHPLAEAVAVYGNRIIAVGATSDIRQLAGTATRVIDAHGQLVLPGFNDAHVHFMSG
                     GFQLSSVDLRDANTPEELAQRIRDFAAKLPEGRWMTGGDWDHERWPGAKLPTKDLIDK
                     FTPKTPVFVNRLDGHMALANSLALKLAGVTHEAKDPPGGLIVRDPNTGEPTGILKDAA
                     QNFVLKVIPAPSFEEKLAAARAATNHAASLGVTSVQDMSAAADVSVYQTLLDRGELKT
                     RIHAVWPLPRWQELAATGVRAHFGNSMLRTGGLKGFSDGSLGSTTALFFEPYLDAPNT
                     HGIPADEMFPEGAMLERVRGSDRAGLQVMIHAIGDRANDTILSIYEQVAKENGARDRR
                     FRIEHAQHLRPQDVGRFGRDKVIASMQPYHAIDDGRWAEKRIGPERAKTTYAFRSLLD
                     SGATLAFGTDWTVAPLNPLLSVYAAVTRRTLDGKHPNGWIPEQKISVEEAVRAYTVGS
                     AFAEFEEKDKGTIAPGKLADIVVVSRNIFEIEPNEIDKAKVVLTMMDGRVVYEASRQ"
     gene            5894..6358
                     /gene="accB"
                     /locus_tag="VFO30_06550"
     CDS             5894..6358
                     /gene="accB"
                     /locus_tag="VFO30_06550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9418984.1"
                     /translation="MELKEIKAIIDLMKKNDLSLFEMEKDGFRLKLQKGALDQPIITA
                     PIAVAPAASATQPAPGEMPAQAPATASTTESTPLKDIVSPMVGTFYRSASPDAPSFVD
                     VGTTVGEDTVVCIIEAMKVMNEIKAETSGVIAEVVAENAQPVQFGEVLFRVR"
     gene            6417..7805
                     /gene="accC"
                     /locus_tag="VFO30_06555"
     CDS             6417..7805
                     /gene="accC"
                     /locus_tag="VFO30_06555"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9418985.1"
                     /translation="MFEKVLIANRGEIALRVIRACKELGIRTLAVYSEADVESLHVQL
                     ADEAICIGAPPSGDSYLKIDRIMSAAEVGDVDAIHPGYGFLAENAHFAEVCESCKIKF
                     IGPPAQAMRLMGDKNAARVTARKAGVPITPGSDGVVENEQSAAKIAKKIGYPVMIKAV
                     AGGGGRGMRTAHNEPSLVAAFHSARLEAEKAFGNSDVYIEKLIVNPHHIEFQIMADRH
                     GRVVHLGERDCSIQRRNQKVIEECPSPLMSSGLRKKMGNAAVKLAKAVGYENAGTIEF
                     LVGEDRHFYFMEMNTRIQVEHTITEEVYECDLVKEQIRIAAGQPLSQHIAHASPHAHA
                     IQCRINAEDPANNFQSSAGRIDFYSAPGGRGVRIDSHAYTGYVVPPYYDSLIAKIIAV
                     AATRLSAIDRMHRALDEYYITGIKTTVPFHAAIMRNAEFREGKYDTGFVERVMASKDF
                     QLAHTPARRLHE"
     gene            7873..>8057
                     /locus_tag="VFO30_06560"
     CDS             7873..>8057
                     /locus_tag="VFO30_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418986.1"
                     /translation="MKHLSDWRLYGILDLSYVDVANIAPAAEAMIAGGVDVIQLRAKA
                     HSIRQIVDLTKGLHQITS"
BASE COUNT         1796 a         2278 c         2344 g         1639 t
ORIGIN      
        1 ccaacgcgcc accgcacgct caaatgcggc gcgcgtgata gcgagtccga ttaagacaac
       61 aatgccagcg tcgacgagga gtttggtcac atgattgagc acgtaatggc ccatctcgtg
      121 cgccatcact tcgcggatct cgggtaatgt gcattgacgc agtagattgt cgttgagcgc
      181 gatccgcgtc gtgccaagaa accccgctac attcgcgccg actcgtttcg tctggcgcga
      241 ggcgtcaacc tcgaacacct gtgtcaccgg aatttgattg gcgcgcgcca tcgcgaggat
      301 tggctctttg atggcgggat cggtgagcgg tttgtaggtg ttaaagagcg gttcgatgta
      361 aatcggcgca atgaacaaac cgatcattcc aaacacgacg gcgacaatcg cgccccaaac
      421 cggccatgtc cgcggagcac gctggaaaac ggcgtaaagc gcgacgagtg aaattgtgcc
      481 gagcaccagc agcagggcga gcccaatgca ttgatcgccg aaccactgag caaaaatttg
      541 cgtcgccatt ccatattgat gttcgcgaaa gaagttctga tagagattga gcgggaagct
      601 gagcgtgcat gtgatgatca ggtaggcgaa cgagtacaac gctacctgtg cgggtctgaa
      661 gcgcgttagg cgttgcgcgc aatcccgcac gcgagctgaa atgcgtgagg cgagcagaaa
      721 gatggagatc gcagccgcga gcagaaagtt ccaaaggatc aaccaataac cgccttcaaa
      781 gtacgcgtcc gatttggcgc gctggtccgg cggcacgctt gccagccagg cctgcgtagc
      841 tgcagcggga tcgagcgatg ttgccgtttc gctcggagtt gcttgttgat ttgttgtcga
      901 tctttcctga gcatagccgt gcagcggcgc aatgatcaat gccgcggccg cacacatggc
      961 gacatggttc ttcacgccag aggtgataat aaattctgcg gtgaaacaaa agcgcggagt
     1021 caccggtcat tcgactggac gaacaacttg acgccgcgca gaatgaacgc cgtgaaaatg
     1081 cgcgtattgg tggtttgcgc actgctggtg ggcggagcgt ctggtgcact gacggagacg
     1141 cgcacgccag acccggagtg gtacggcccg tatccggcca attacaaaga gatcgtcacc
     1201 aagtggctgg aaacgcaatt gctcgacccg acgagcgcgc gcatcgaatg gaaagacgag
     1261 ccgaagccag ccgatttagg ctcgaaaggc gaacatctct atggttatct ggttaccttc
     1321 accgttaacg cgcgaaaccg ctttggcacc tacacgggca aacaggtaca tggtgcgctg
     1381 attcgcaacg gcgaagtaat taaaggcgtt ggattcggtt actagaatat gaaactacct
     1441 aacaaattgc aaatccgcga agccggccaa gccatcggcg tgttggttgt ccttctcgcg
     1501 atcatcggca tcggtgtatg gctgctctac tcctatcgca acggcatgcg cgatgaaggc
     1561 cgagcctttg ggcgtcaggt cattgagcgc gtcgccgttc aacacgatgc gaatttcctc
     1621 gccacgagtc tcgcgccgca ggcgagattg aacctgccgc cgccgatgca gcaggaactc
     1681 atggatcgct tacgacaact gggcacaccg gtcggccaac cgaacgcgca gggagacgtc
     1741 acgtttgaat cgcagttctt cgaaccgcac ggcgcgtttt atgcccaact caactatccc
     1801 gctggcagca tgcgaatgga cgtggcgatt tctcatccgg tcggacgctg gcaagtcgat
     1861 tccatcaccc tgagcacggt cggggcgcga tagcgattat cgatcttcca agcttggctt
     1921 gctagactcg actgcttaaa gcagtcgagt ttaggcctcg gcgtaccgtc gctaagcaag
     1981 taaacgatcg agcgcgccca cacaaagctg cgaccatgtc tcgagcgagt cgcgccgggc
     2041 gaggagctcc tctttactta ggaacgccag gttcgtaatc atcgcctcgc gtttctcaac
     2101 ctttggctgc gcgagtttga aaacgtgcac aatcccgaga tgtaccagcc caacctcgct
     2161 cgcgtcatca tttagcagtg cgacgatgtg atcttcaaat tgactctcga tcttgatctc
     2221 ttcattgact tcgcgctcga cgccggcgcg gtaggccgcc tggtccagtg caaagagcga
     2281 ttcgtcgcgg tcgttcatgt ggccgccgat cccgattgag cctttcgcaa ccagccgctg
     2341 ttcgccggct tttttgccgc ggacgtaatg aagcaccttg tcgtcaaacg cgaggagcac
     2401 atacgggatg agctgcttgt gcgccgggtc atgctcggct tcggcgcgcg gcaggaaaaa
     2461 attgttcccg cgcctgagca attcacgcag gtatttctcc ggctcgaaat tgagcccttg
     2521 gaaactgccg agctgatcga acaaacttcg tctaaccacc agcacgttct cgttaggctg
     2581 gctcattgcg atgagggcga ctcagaacga gcaggtaatg ccgacctgcg gcccaaatag
     2641 attcaaactc gggttggggt tggtttgccc gccgtttgaa agatgctggt aaagaactcc
     2701 ggcattgacg ctccagcgat catcaatcgt gtaagccagt ccagcggctg acaaaatgtt
     2761 aaacgtgaaa tcctggccct ggccgccggg gatgttggcg tgactgtcga tccaacccag
     2821 acccagcccg cccgaaacgt aaggcaccca ccgactaccg ggcggaacga agttgtagcg
     2881 ccccccgaag ttcagcccga aatagtggtt ctcgattcct ttgaaaatcg gctccgccat
     2941 ggcgctgatg taaaattgat tgtaaccgcg cagccaatca gtgcgctcga gcacacccca
     3001 gcgaacgcgc gccgtcaaaa attcagcgcc gatctgatag gcctgcggcg gattgatggc
     3061 gccgaacagg taacccgatt caagtgcgat ctgaaaccgc ggcggattca actgggctgc
     3121 ggctaaagtg ctcacctcgg ctgggcctgc aaacccgcgc ggtgcgccaa ctgtgaacgc
     3181 gaaaagagcc atgagcacga agcgcttcat cgcgcgctag gatatgagga gtcgtttcga
     3241 ttgcaattcg tagcggcgcg ctccgagcgc cgaaattatt gaacgacgat cagagatccc
     3301 cgctacagcc gatgaactcc gcaagatcca gaggcaacga actgctccat ttgtgttcgc
     3361 cggggatcgc cagtcgggtc gcatgcaagg cgtggcgcgg caagagtaat tgtcgatgta
     3421 acgccggtgt ccacccggtt tcgatgaact ccaggtaaag cctctcgtcc ggtccgtaaa
     3481 ttttgtcacc cacgattgga tgaccgagcg acgcgagatg cacgcggatt tggtgcgttc
     3541 ggccggtacg tggaatcgcg cggattagcg caaatttgtt gccagaagat gtcgatcctg
     3601 cgacttcgct caggacgggt cttgtgaaac gttgctcgat gataaaatcc gttcgcgcgg
     3661 gagcaccggc gggatggatc atttgctcga gccaaatccg ggtgggttga tgtttacctt
     3721 ggcgatcgag tggcgcatcg acaattttgc tgtcgcacgc cggccagccc cacacgattg
     3781 cgagatattc cttctcgaat ccttggaatt gcatcagcag cccaaatcgc cgcgccgcgg
     3841 ccgcggtttt cgccacaagc acgagaccac tcgtttcgcg gtcgagtcga tttacgattg
     3901 agacctgccc gccgttcgcg atttcaaatg cgaggagctc gcgtaattct ttccaaagtg
     3961 ttgtctgctc agtaggtttt gtcggatgcg agagcagaaa cggcggttta tcgacgacaa
     4021 cgtagtcttc actctcatcc acgaccgcaa atcgcggagc aacgccgcgg tgcaccgatg
     4081 atgaagcgga agtcactttc tgtgctactg acgttcttcg gctgtatgtc aatcagcttt
     4141 tctgccggcc ctgagaagca ggcgttctcg gctgacttga tgatcatcaa tggcgtggtc
     4201 cacacgatgg attcagcgca tccgctggcc gaggcagtcg cggtttatgg aaatcgaatc
     4261 attgctgtcg gagcgacatc ggacatccgg cagctggcgg gcacggcgac gcgcgtcatc
     4321 gacgcgcatg gccagcttgt tcttcctggc ttcaatgacg ctcacgtcca tttcatgagc
     4381 ggcggttttc agttgtccag tgtcgatcta cgcgacgcga acacgccgga ggagcttgct
     4441 cagcgcatcc gcgatttcgc tgccaaactg ccagagggca ggtggatgac gggcggcgat
     4501 tgggaccatg agcgctggcc tggcgccaag ctgccgacga aagatttgat cgacaaattc
     4561 acgcccaaaa caccggtgtt tgtaaatcgg ctcgatggcc acatggcgct ggccaacagt
     4621 ttggcattga aactcgcagg cgtgacgcac gaggcaaaag atccgccagg cggattgatc
     4681 gtgcgcgatc cgaacaccgg cgagccgacc ggaattttga aagacgcagc gcagaatttt
     4741 gtcttgaagg taattccggc gccgagtttt gaagagaaac tcgcggctgc gcgcgcagcg
     4801 acaaaccacg ccgccagctt gggcgtgacc agcgtgcagg acatgtccgc ggctgcggac
     4861 gtttcggttt accaaacgtt gctggatcgc ggcgaactca agacgcgaat ccatgcggtc
     4921 tggccgttgc cgcgttggca ggagctggct gccaccggtg tgcgcgcaca ttttggcaac
     4981 tcgatgctgc gcactggcgg cttgaaaggt ttttccgatg gaagtcttgg ctcgaccaca
     5041 gcgcttttct tcgagccgta tctcgatgcg ccaaacaccc acggcatccc tgctgatgaa
     5101 atgtttcccg aaggtgcgat gctcgagcgc gtgcgtggca gcgaccgcgc gggcctgcaa
     5161 gtgatgattc acgcgatcgg tgaccgcgcg aacgacacga tcttgtcgat ctacgaacag
     5221 gttgcgaaag aaaacggcgc gcgcgatcga cgttttcgca ttgaacatgc gcaacatttg
     5281 cgcccgcagg acgtcggacg attcggtcgc gacaaagtca ttgcgtcgat gcaaccgtat
     5341 cacgcgatcg acgatggccg ctgggctgag aaacgcatcg ggccggagcg ggcaaagaca
     5401 acttacgcat tccgatcgct gttggactcc ggcgcgactc tcgcatttgg cacggactgg
     5461 acggtcgcgc cactgaatcc gctgttgtcg gtctacgctg cagtgacacg tcgcacactc
     5521 gacggaaaac atccaaacgg ctggatcccc gaacaaaaga tttcggtgga ggaagccgtt
     5581 cgtgcttaca cggtcggctc cgcgttcgcg gagtttgagg aaaaagacaa aggcacgatc
     5641 gcccctggca aactcgcgga catcgtcgtc gtttcgcgca acatttttga gatcgagcct
     5701 aacgaaattg acaaagcgaa ggtcgtcctc acgatgatgg acggccgcgt ggtctatgaa
     5761 gcgtcgcgcc aatgaaatat cctctgcgcg cgttcacgag cggcacgtac cgtcacgctc
     5821 gctaccgctg gcttgacctc gattgcaaaa gcgctttacc tagcggctcg gtttccactt
     5881 cgaaggaggc accttggagc tcaaagagat caaagccatc atcgatttga tgaagaagaa
     5941 cgacctctcg cttttcgaga tggagaagga cggctttcgg ttgaagcttc agaaaggcgc
     6001 actcgatcag ccaatcatca ccgcgccgat cgcggttgcg ccagccgcct cagcaaccca
     6061 gccggcgccg ggtgaaatgc ccgcacaagc accggctact gcttcgacaa ccgagagcac
     6121 gccgttaaag gacatcgttt ctccgatggt cggcacattc tatcgatctg cttcgccgga
     6181 cgcgccgtca tttgtcgatg tcggcacgac ggtgggcgaa gacacagtcg tttgcatcat
     6241 cgaagcgatg aaagtgatga atgaaatcaa agccgagacc agcggagtta tcgccgaagt
     6301 cgtcgccgaa aacgcgcagc ccgtgcaatt cggtgaggtc ctgtttcggg taaggtagcg
     6361 caagcttcca gcttgcattt tccctgaacg caagcgaagc gcttgcccta caattcatgt
     6421 tcgagaaggt cctcattgcg aatcgcggcg agatcgcgct gcgcgtcatt cgcgcgtgca
     6481 aggaactcgg aatccgcacg ctggcggttt attccgaagc ggatgtcgag tcgctccatg
     6541 tgcagctggc ggacgaggcg atttgcatcg gtgccccacc cagtggggac agctatttaa
     6601 agatcgaccg gatcatgagc gcggcggaag taggcgacgt tgatgccatt catcccggct
     6661 atggttttct cgccgagaac gcgcacttcg ccgaagtgtg cgagagctgc aaaatcaaat
     6721 tcatcggccc gccggcgcag gcgatgcgtt tgatgggcga taagaacgcg gctcgcgtta
     6781 cggcgcggaa agccggtgtg ccaatcacac cgggaagtga tggagtggtg gagaacgagc
     6841 aaagcgcggc caaaatcgca aagaaaatcg gatacccagt gatgatcaaa gccgtagccg
     6901 gtgggggtgg ccgcggcatg cgcaccgcgc acaacgagcc gagtctggtt gcggcctttc
     6961 acagcgcgcg tttggaggca gagaaagcgt tcggcaattc ggatgtctac atcgaaaaac
     7021 tcatcgtgaa tccgcatcac atcgagttcc aaatcatggc cgatcggcac gggcgcgtcg
     7081 tgcatctcgg cgagcgcgat tgttccattc aacgccgcaa tcaaaaggtg atcgaggaat
     7141 gtccatcacc gttgatgagt tcgggtctac gcaaaaaaat gggcaacgcc gccgtgaaac
     7201 ttgccaaagc cgttggttat gagaatgccg gtacgatcga gtttctggtc ggcgaagatc
     7261 gacattttta cttcatggag atgaacacgc gcattcaagt cgagcacacg atcaccgagg
     7321 aagtttacga gtgcgatctg gtgaaagagc aaatccgcat cgccgccggc cagccgctgt
     7381 ctcagcacat cgctcatgct tcgccgcacg cacatgcgat tcaatgccgg atcaacgcgg
     7441 aagatccggc caacaatttt cagtcatcag ctggtcgaat cgacttttat tccgcgcccg
     7501 gcggccgcgg cgtccgcatc gattcgcatg cctacaccgg ctatgtcgtt ccaccctatt
     7561 acgattcgct gatcgccaaa attatcgccg tcgccgcgac acgactttcg gcgatcgacc
     7621 ggatgcatcg cgcgctcgac gaatactata tcaccggaat caaaacgacg gtgccatttc
     7681 acgccgcgat catgcgcaat gccgagtttc gcgagggcaa gtacgacacc ggctttgtcg
     7741 agcgcgtcat ggcctcgaaa gatttccagt tggcgcacac gcctgcgcgc cgcctgcatg
     7801 agtaagtcgt agccacggct cgttgagccg tcctgaggcg gcgcacagcg ccgccgctac
     7861 agtacgcgcg acatgaagca tctcagtgac tggcggctat acggcattct cgacctcagc
     7921 tatgtcgatg ttgccaacat cgcgccagcg gctgaggcaa tgatcgccgg tggtgtcgat
     7981 gtcatccaac tacgcgcgaa agcgcattcg attcgacaaa ttgtcgatct tacaaaaggt
     8041 cttcaccaga tcacatc
//
LOCUS       DASTDG010000204        15463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_336337, whole genome shotgun sequence.
ACCESSION   DASTDG010000204 DASTDG010000000
VERSION     DASTDG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15463
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336337"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1030)
                     /locus_tag="VFO30_06565"
     CDS             complement(152..1030)
                     /locus_tag="VFO30_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HET9418987.1"
                     /translation="MTNVIDGRAIAEKVYVDLRREIAQLKSRGVTPGIAVVLVGDNPA
                     SRAYIRSKDRMSRELGLHSVKLELPAATTQEELLGRIDHLNRDPAIHGILVQSPPPPQ
                     IDEASVVRALDPRKDVDGFHPVNVAKLVLGDATGFVPCTPLGVQRLLTESRIDITGAH
                     VVILGRSMIVGKPLALVLMQKANGADATVTVVHSRSRNLTEITRSAEILIAAIGRANF
                     VKAEHVRDGAVVIDVGINRVEDAATERGYRLVGDVDFESVAPKAAAITPVPGGVGPMT
                     IAMLMSNTVKAARQLP"
     gene            complement(1048..1692)
                     /locus_tag="VFO30_06570"
     CDS             complement(1048..1692)
                     /locus_tag="VFO30_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9418988.1"
                     /translation="MKIQGRPARWLIVFAVGLLRIWERTLRFDLDDRAGIAGKPVTQN
                     YIGALWHNRLLVFPLVLRRFFPNRKGAALISASRDGDLLTDAIARFGFDVVRGSSSRL
                     GASALLQLSDTLAAGRDVVITPDGPRGPAYELGPGIIFLAQKTGAPVLPMNLEYSSCW
                     RFKSWDRFIVPRPFSKVRVLIGQPQHVQSTTTNDEFEAERVRLQIAMMSLVEMR"
     gene            complement(1689..2249)
                     /locus_tag="VFO30_06575"
     CDS             complement(1689..2249)
                     /locus_tag="VFO30_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET9418989.1"
                     /translation="MRLVIAATGASGTIYLQRLLEQIDCTAHEVHLVMSAYARQVAAQ
                     EMDEFKAPAGVSEHDDADMNVPYVSGSARFDAMVIVPCSMSTLGRIASASGDNALLRA
                     ADVFLKEGRKLVLVPRETPWHLIHARNVLTLLEAGAVVLPAIPSFYSRPKTILDLVDT
                     VVWRILDQIGLPSPRTYRWPDQPKPS"
     gene            2302..2688
                     /locus_tag="VFO30_06580"
     CDS             2302..2688
                     /locus_tag="VFO30_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9418990.1"
                     /translation="MVKKLLHTRYRVHDLEKTVGFYKDVLGLHETRRHTSGRGSQLVF
                     LKAPGSEEEIELCKFDESGPVVVGPDLTHLAFEVDDIEKFAREAAAKGYPLSDGPHHT
                     AGGSVIAFIDAPEGYEIELIQRGGDM"
     gene            2747..3688
                     /locus_tag="VFO30_06585"
     CDS             2747..3688
                     /locus_tag="VFO30_06585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9418991.1"
                     /translation="MRRFPLCVVFCTIALCAFAVTPPDDEDNPNAVRPTGVTHRHSAK
                     RTHHATAKKEEPEDVPIVTNVPGVVPNTSASSAIVVDAHNGEVLYEKNADQYRQPAST
                     QKLLTALIIVETGYLDQPVTVAPIDTMAEPVKLNIRPGETYRRIDLLRALLVKSPNDV
                     ARCLARDNAGSIEAFAERMNQRARELGALHSHFVNPNGLPTPGQYSTARDLSIIARAA
                     YANSTIRSIVCLPRVVFRFANGRTRELENTNKLLQRLPYCNGMKTGYTEAAGKCLIAS
                     GARPGKDVIVVVLGDGSGRIWRDAGSLLAWGLWNPAG"
     gene            3685..4863
                     /gene="bioF"
                     /locus_tag="VFO30_06590"
     CDS             3685..4863
                     /gene="bioF"
                     /locus_tag="VFO30_06590"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET9418992.1"
                     /translation="MSDWFAEQLDQLRERSLDRHLREISSAQGPLIELAGKRLINFSS
                     NDYLGLANDARLRDAAMTALKEFGVGTGASRLVSGTQSPHVQLEAALANWKGTEAALT
                     FSSGYAAALGTIPALVTQGDVVVLDKLCHASLIDGAKLSGAILRVFPHSDMGKLESHL
                     QWAEREHPGKRVLVVTESVFSMDGDRAPLREIVELKTHFGALLLLDEAHAVGVLGRQG
                     RGLAATEQLNEQVDVQMGTLSKALGVTGGYICGSRSLIDWLINRARSFIYSTAPLPSA
                     AAAARAAVEFLASRDGETRREQLWRNIDILHDALSTKPASAILPWIIGDEKAAVEAAA
                     TLRAEGFLVPAIRYPSVAKGSARLRITVTAAHSEEQIRALCAVIAQLRGETQREPITV
                     "
     gene            complement(4853..5116)
                     /locus_tag="VFO30_06595"
     CDS             complement(4853..5116)
                     /locus_tag="VFO30_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418993.1"
                     /translation="MKIQIAICLLSLALLPACSTTQMTAQHQTLEQRLMAKYAYAETA
                     AGEPAPPAEGPEDLPADAPQNVDRNPNLVPTPLLRVSAASATP"
     gene            complement(5196..6065)
                     /gene="mtnP"
                     /locus_tag="VFO30_06600"
     CDS             complement(5196..6065)
                     /gene="mtnP"
                     /locus_tag="VFO30_06600"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET9418994.1"
                     /translation="MEDEAPAIGIIGGSGLYQMEELRDPTEHKIDTPFGPPSDALISG
                     TISGRQVYFLSRHARGHRILPHELNHRANVWALRSLGVRWIVCVTAVGSLQEKYAPRD
                     VLLPSQFYDRTSFRHAHTFFGNGIAAHVSFADPISSSLRNLVAESARSLGITVHNGGT
                     YVNMDGPAFSTRAESELNRRHGFDVIGMTNLPEAKLAREAEIALATMAMITDYDCWKI
                     EEEPVSAQTVFEHLLVNAETAKRVIVDLMPRIPREPNWPEHFALDAALVTERKLWPAA
                     TVGKLRPILGRFL"
     gene            6153..7559
                     /gene="miaB"
                     /locus_tag="VFO30_06605"
     CDS             6153..7559
                     /gene="miaB"
                     /locus_tag="VFO30_06605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9418995.1"
                     /translation="MPKFFIKTYGCQMNERDSEQVAHSLLSRGYERVHDESQADVVLL
                     NTCSVRDMAEQKALGKMGMLGRVAKERPHVVFGFLGCMAQARGASLLRNLPHVDLVVG
                     TQKFHRVADYVEDLVLQKRALVAGVADPGYRRIDDLRFSIVDVADEPGSQSTIREQQL
                     APKQATAFVSIMQGCNMHCTFCIVPQTRGAERSRTIEEIVDEVRDLVARGVKEVTLLG
                     QIVNLYGRHEFPKVDNQSPFVQLLEAVHDTQGLERLRFTSPHPIGFRDDLIDALARLP
                     KLMPHVHLPLQSGSNRILKAMHRTYTSEKYVDLVERIRRARPGISITTDVIVGFPGES
                     DSDYEQTRGLVERIEFDNAFVFRYSPRRDTPAAAMPNQVDELIKEQRNQDLLTIVNAS
                     ARRAGERLIGQEVEVLCEGRSKTNSARLMGRTPTNKIVVFEGGNELVGGLVDVRVERA
                     TGFSLYGTPRVSCATLAA"
     gene            7556..8104
                     /locus_tag="VFO30_06610"
     CDS             7556..8104
                     /locus_tag="VFO30_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9418996.1"
                     /translation="MIYHLSLRAAGGAVGAALVLLSLPGLFKPDLAAAVQKFPRSRLA
                     GFIVLTVDLVWTMWLLATIEMGEFSSFRRPLLIAVPIGYVLALRFVDEFLAVRALGIF
                     CLLGAEPLLDAAFLRYELSRLLVTVFAYALVVVGLFWVMMPYLLRDQINWSAQSTPRW
                     RTVHAVCFVYGAAILAFAFTQY"
     gene            8101..9051
                     /locus_tag="VFO30_06615"
     CDS             8101..9051
                     /locus_tag="VFO30_06615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9418997.1"
                     /translation="MKRILVIRGGAIGDFVLTLPALRALREAYSEAYLEILGYQQIAT
                     LAAIASYADAVRSIEYGPLSSFFARNAELPGELAAHFNSFDLILSYLYDPDRIFESNL
                     RRAGVENLIIGPGKLANCEHAAQQLARPLQELGIYVSKFAAQISPSIQDRHLASEFLH
                     ACVEPIVVLHPGSGSEKKNWPLANWIELGNDLLGAESFGGSIVIVAGEADAAQVGQLE
                     TTWQTGRVRFAKNLPLPLLAAILDQTVFIGHDSGISHLAAAAGAHCILLFGPTDPKVW
                     APRNDNVRLIQAPDGDLRALDVDLVRAELVQELMRIGIST"
     gene            complement(9018..10115)
                     /gene="dnaN"
                     /locus_tag="VFO30_06620"
     CDS             complement(9018..10115)
                     /gene="dnaN"
                     /locus_tag="VFO30_06620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET9418998.1"
                     /translation="MKFTVTKEKLLEGLQQVQNVVSTRTTLPILSNVLLQANGKEVHL
                     TTTDLDVGVRGSFEADVEKSGATTLPARRLFNIIRELPSSEIHFDVDGKNTASIRSGQ
                     SFFKILGLPEEEFPPLPKFEDAKVVTMRQKDLRDGLRKTSYAISTDETRYVLNGVLFS
                     FKENKLTLVATDGRRLAMVDIELEFPRSHEADIIVPTKAVTELQRLLTDNGDVKVSMG
                     SGQIAFDVNNTLLVSKLIEGNYPNYKQVIPTEAKERVTLERETFLNSLRRVSLLASDK
                     SNSIKLNFSKNNIEITANTPEVGEAKESLPVAYKGRDFSIAFNPEFLMAPLRALTEDE
                     VYLDLIDEMSPGVLKIQTPFLYVLMPMRISS"
     gene            complement(10176..11525)
                     /gene="dnaA"
                     /locus_tag="VFO30_06625"
     CDS             complement(10176..11525)
                     /gene="dnaA"
                     /locus_tag="VFO30_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9418999.1"
                     /translation="MKDKCAEFWQRLAAALKPQVSADTFKRWFSAVELAEATDTRLTF
                     RVPNNIYQFWIESNHMAALQAAIMTAFGGPREVKFVFPGNSGEVAEDAIVSQRPETSQ
                     ETKSSGSALGLNPRNRFETFVVGPNNEIAHAASLAVAQAPARTYNPLFIYGGVGLGKT
                     HLMQATGDFVWRKKKSTKVIYVSSELFINEFIDAIQHSNLVKFRKRYRQADVLLIDDI
                     QFLAGKERSQEEFFHTFNTLFDGHKQIVLSSDRPASEIANLEKRLVSRFEWGLTAELQ
                     PPDIETRMAILRMKARAMQIKLSDDIFQFLANRIRSNVRRLEGALMRVASFASLSGKE
                     LTHDVVEHLLKDILQEEARHAITIEQIQRRVAEHFDVRLADMTSKRRPANIAFPRQVA
                     MYLARELTKASLNEIGDAFGGRDHGTVLHACKLVKKRMKEQDSLRQTISFIDSSLQR"
     gene            12064..12666
                     /locus_tag="VFO30_06630"
     CDS             12064..12666
                     /locus_tag="VFO30_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419000.1"
                     /translation="MKLQQNQIANCRRKLLTRADIPVVLSTRTGLALSLAVFFALSGI
                     KTSAIEELLPPFGFRWNDPVSRVEAVLEGAKARIVSREKKEGREVWTVEGLVHPGLKR
                     TLFTFKGGALFGVELQYEYPDWSIERYNQRMGEIRKYFDDKYGTGKLVSRARDTDSDV
                     VQTLVGYQWMVGATMLELFYFSAQHDALVYRTITVDYKAL"
     gene            complement(12732..13337)
                     /gene="def"
                     /locus_tag="VFO30_06635"
     CDS             complement(12732..13337)
                     /gene="def"
                     /locus_tag="VFO30_06635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9419001.1"
                     /translation="MKLSILRYGDPMLRAKGRRIEKTDNHIRELATNMLETMHAANGI
                     GLAAQQIGEALQLTVIDVSQAEDRPSTMRINGEEVDPKSAMPLVLINPELELRGETEL
                     GLEGCLSFPEITGEIERAGSVIARAQTLDGEIEFEAGGLLARALQHEIDHLNGVLFID
                     RMNSAAKAVLASRLKRLQKETKRGQRVRAQDRQLQPSDTVL"
     gene            complement(13334..13741)
                     /locus_tag="VFO30_06640"
     CDS             complement(13334..13741)
                     /locus_tag="VFO30_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9419002.1"
                     /translation="MNFAVRKRRAPTIIIVSLVDILIILLIFFVVSTTFKKDQPEVQI
                     NLPESKTATKKPAELEHVIVSVDESDQIQLDGRAVDVDQLEAAVRELPETRKSTLALQ
                     ADRKASWGTIVKVMDALKLAGVKNLPSFTREPQ"
     gene            complement(13764..14477)
                     /locus_tag="VFO30_06645"
     CDS             complement(13764..14477)
                     /locus_tag="VFO30_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9419003.1"
                     /translation="MALADMSEITQTVLGFFTKGGLFMWPLLACSIVSVTTMILRGLA
                     LREKNVMPSLIQSEIERLVPGASAERLARIVAHDPSSLARIARVALQHLRAPRSENIE
                     VVETRARHEMVRLEKGLIVLEVIVGIAPLLGLIGAVSGLVHVFSHLGLSSGASDTRQI
                     ALGIAEALNATVFGLSIAVPTLIGFSYFSKKVEVMSVEMETLVVELINKCYYGRSSRE
                     FVPTKTSPPPTVTVPTPVA"
     gene            complement(14486..>15463)
                     /locus_tag="VFO30_06650"
     CDS             complement(14486..>15463)
                     /locus_tag="VFO30_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419004.1"
                     /translation="MTTAEMMFAQPGDTLLDRKHEFRKVFWAFVAALLIHLLVAFGLA
                     AVGGAFSSAPSIEDKPAELTIVDLAPATVPKNPAFIETDESKNAPVPQEKTFESNANS
                     IGASQLPATGDLPVPSQEGKERPAVDLETHQYSLANQGVQPQPSAVPNETPQPSQAPE
                     TTPQPTPLSESEQFAMLTSTPNPTVQPSVAPTPQQPASTYRAEKQQTRLAGSITNRGA
                     PSVNAVGTPLGRYQKLLYDAVGSRWYYYVNKQRDLVSIGTLRLVFSVGRDGKPKNLKV
                     VANSSNESFANVCLQSVLEIELPPLPDDVASTVPPEGLDEEMSFTMYAN"
BASE COUNT         3132 a         4458 c         4546 g         3327 t
ORIGIN      
        1 cttgcgcgcg gggcggtatt gtcaccgcga attgcatcgg cgttcgtgca cgcggcgcga
       61 attcaaccgg ctctcctgcg actgcccacg cgaaaaattg tagccacggc tctatgagcc
      121 gtctgacccg agacgcgcca catgcgcgtg gctacggtag ctgtcgcgcg gccttgacgg
      181 tgttggacat caacatcgca attgtcattg gcccgacgcc gcccggcacg ggcgtaatgg
      241 ccgcagcttt cggcgcgaca ctttcaaaat cgacatcgcc gacgaggcga taaccgcgct
      301 cagttgcagc gtcttcgacg cgattgatgc cgacatcgat cacgacggcg ccatcgcgga
      361 cgtgctcggc tttgacgaaa ttggcgcggc cgatcgccgc gatcaggatc tcagccgaac
      421 gtgtgatctc ggtgagattg cgactgcgcg aatgaacgac ggtaactgtt gcatccgcgc
      481 cgttggcctt ttgcatgagc acaagcgcca gcggttttcc gacgatcatg ctgcggccga
      541 gaatcacgac gtgcgcacca gtgatgtcga tcctgctttc ggtgagcagc cgctgcacgc
      601 caagcggggt gcacggcaca aaaccagtcg catcgcccaa cacgagtttg gcgacattta
      661 ccggatgaaa gccatctacg tctttacgtg ggtcgagcgc gcgcacgacc gaggcttcat
      721 cgatctgcgg cggcggtggc gattgcacga ggattccgtg aatcgccggg tcgcggttca
      781 ggtgatcaat ccgcccaagt aattcctcct gagttgttgc cgccggaagc tccagtttta
      841 ccgaatgcaa gccgagttcc cggctcattc tgtctttcga gcggatgtaa gcgcgcgacg
      901 cagggttgtc gcccacgagc acaaccgcga tcccgggcgt gacgccgcgc gatttcagct
      961 gagcaatttc gcggcgaaga tcgacgtaca ctttttcggc gatcgcgcgg ccgtcgatca
     1021 cattcgtcat gtgcgaaggt aaatgcgtca gcgcatttca accagcgaca tcatggcgat
     1081 ctgcaatcgg acacgctcag cttcaaactc atcgttcgtg gttgtcgatt gaacgtgctg
     1141 aggctggccg atgagcaccc gcactttcga gaatggccgc gggacgataa agcggtccca
     1201 gcttttaaaa cgccagcagc tcgaatactc caggttcatc ggcaacactg gcgcgccggt
     1261 cttctgcgcg aggaaaatga ttcccggtcc gagctcataa gctggcccgc gcggaccatc
     1321 cggcgtaata acgacgtcgc ggccagcggc cagcgtgtcg ctgagctgaa ggagcgcgct
     1381 cgcgcccagc cgcgagctcg atccgcgcac aacgtcgaag ccgaagcgcg caattgcgtc
     1441 ggtgagcaga tcgccatcgc ggctcgcgct gatcagtgct gcgcctttgc ggtttgggaa
     1501 aaatcgccgc agcaccaaag gaaaaacaag cagccgattg tgccagagag cgccgatgta
     1561 gttttgggtc accggtttgc cggcgatgcc ggcgcgatca tccaggtcga accgcagcgt
     1621 gcgttcccaa atacgcagca aaccgactgc aaacacgatg agccagcgcg ctggtctgcc
     1681 ctgaatcttc aactcggctt cggttgatcc ggccagcggt aggtgcgcgg gcttggtaac
     1741 ccgatctggt cgagaatgcg ccaaacgacg gtgtcgacca gatcgagaat cgtctttggt
     1801 cgactgtaga aggatggaat cgccggcaac actacggcgc cagcctcgag caacgtcagg
     1861 acgttgcgtg cgtgaatgag atgccacggc gtctcgcgcg ggacgagcac gagtttgcgc
     1921 ccctccttga ggaaaacgtc ggcggcgcgg agcagtgcgt tgtcgccgga ggcggacgcg
     1981 atgcgaccga gcgtagacat tgagcacggc acgatgacca tggcgtcgaa gcgcgccgaa
     2041 ccactcacat atggcacatt catgtcggca tcgtcgtgct cggacacgcc tgcgggagct
     2101 ttgaattcgt ccatttcctg cgcggcgacc tggcgtgcgt aggcgctcat gacgaggtgt
     2161 acttcgtgcg cggtacaatc gatctgctca agcaagcgtt gcaggtaaat cgtcccgctc
     2221 gcgccggtcg ccgcaatgac aagcctcatg gaagtgcgcc gaatttgaat ttaatcgcgc
     2281 acgggagcaa taagatacct catggtgaaa aaactcctcc acacgcgcta ccgcgttcat
     2341 gatctggaaa aaaccgtcgg cttctacaag gacgtactcg gcttacacga gacgcgtcgc
     2401 cacacatcgg gccgcggctc gcaactggtt tttctaaagg cgccgggcag cgaggaagaa
     2461 atcgagctgt gcaagttcga cgagagcggt ccggtagtgg tcggacctga cctgacgcac
     2521 ctcgcgtttg aagtggacga catcgagaag ttcgcgcgtg aagctgcggc gaagggctat
     2581 ccactctccg atggcccgca tcacaccgcg ggcgggagcg tgatcgcgtt tatcgacgcg
     2641 ccggaaggtt acgagatcga gctgattcag cgcggcggcg atatgtaggg attggccggc
     2701 caactgcttg cgtatcggaa gtagataccg ctaatttccc gccgacatgc gccgttttcc
     2761 cctctgtgtc gtgttctgca caatcgcgct ttgtgcgttt gcagtaacgc caccggatga
     2821 cgaagacaac cccaacgccg taaggcccac cggcgtcacg catcggcaca gcgcgaagcg
     2881 cacccaccac gcgaccgcta aaaaagagga gccggaagat gttccaattg tcacgaacgt
     2941 gccgggagtg gtgcctaaca ccagcgccag ctcggccatc gtcgtcgatg cccacaacgg
     3001 ggaagtgttg tatgagaaaa atgccgacca ataccggcag cccgcgagca cacaaaaact
     3061 gctgactgct ctgatcatcg tcgaaacggg atatttggat cagccggtca cagttgcacc
     3121 aatcgacacg atggctgagc cggtgaaact gaacatccgg ccgggcgaaa cgtatcggcg
     3181 cattgacctg ctgcgcgcgt tgctcgtcaa aagtccgaac gatgtcgcgc gctgcctggc
     3241 acgcgataac gccgggagta ttgaagcttt cgccgagcgt atgaaccagc gtgcgcgcga
     3301 gcttggcgcg ctccactcgc attttgttaa cccgaatggg ctgccgacac ccggccagta
     3361 ctcaacggcg cgtgatttgt cgatcatcgc ccgcgccgcg tatgcgaact cgaccattcg
     3421 ctcgattgtc tgcttgccac gggttgtatt tcgctttgcc aacgggcgca cgcgcgagct
     3481 ggaaaacacg aacaagcttt tgcaacgact tccgtactgc aacggcatga agaccggcta
     3541 caccgaagcg gccggcaaat gtctcatcgc cagcggtgcg cgccccggca aagacgtgat
     3601 cgtggttgtg cttggcgatg gcagtggccg tatctggcgg gatgccgggt cgttgctcgc
     3661 ctggggactc tggaatccag ccggatgagc gactggtttg cggagcaact cgatcagctc
     3721 cgcgagcgct cgttagatcg gcatctgcgc gagatcagta gtgcgcaagg accacttatc
     3781 gagcttgcgg gcaaacggct catcaatttc tcttccaacg attacttggg actcgcgaat
     3841 gatgcgcggt tgcgcgatgc ggcgatgacc gcgctgaagg aattcggcgt tggcacggga
     3901 gcttcgcgtt tggtcagtgg gacgcaatcg ccccacgtgc aattggaagc agcgcttgcg
     3961 aattggaaag gcacggaagc ggcgctcacg ttcagcagcg gttacgcggc tgcgctcggc
     4021 acaattccgg cgctcgtcac acagggcgac gtcgttgtcc tcgataagct ttgccacgcg
     4081 tcattgatcg acggggcgaa gctgagcggc gcgatcctcc gtgtctttcc gcatagtgac
     4141 atgggcaaac tcgagagcca tctgcaatgg gctgagcgcg aacatccggg caagcgcgtc
     4201 cttgttgtca cggaatctgt tttctccatg gacggcgatc gcgcgccgct gcgcgagatt
     4261 gttgagttga agacgcattt cggcgcgctg ttgctgctcg atgaggcaca cgctgtcggg
     4321 gtgcttggtc gccagggacg cggcctcgcc gcgaccgaac agttgaatga acaggtcgac
     4381 gtccagatgg gcacgttgag caaagccttg ggtgtgaccg gcggctacat ctgcggctcg
     4441 cgcagcttga ttgactggct gatcaatcgc gctcgctcgt tcatttactc gaccgcaccg
     4501 ctaccgtcag cggcagcggc ggcgcgtgcg gcggtggaat tccttgcttc gcgcgatgga
     4561 gaaacgcgac gggaacagct ctggcgcaac attgacattt tgcacgacgc gctgtccacc
     4621 aaaccggcga gcgcgatttt gccgtggata atcggcgatg agaaagcggc agtcgaagcc
     4681 gcggcgacgc tccgcgccga aggatttttg gtgccagcaa ttcgttaccc gagtgtcgcg
     4741 aaaggctcag cgcgcctgcg aattaccgtg accgcggcgc acagcgaaga gcagatccgg
     4801 gcgttatgcg cagtgattgc gcaactacgc ggtgaaaccc aacgcgagcc gattacggtg
     4861 tagccgaagc cgcggaaacg cgaagcaacg gagttgggac aaggttcgga ttacgatcga
     4921 cattctgcgg cgcatcggcg ggaaggtcct ccgggccttc agccggtggc gcgggttcgc
     4981 cggcagccgt ctccgcatag gcgtactttg ccatgaggcg ctgctcgagt gtttgatgct
     5041 gggcggtcat ttgcgtggtt gagcaggcgg gcagcaatgc gaggctgaga aggcagatgg
     5101 cgatttggat tttcatgcgt ggactcggat ttgtgtcgtt atcggctgga ctataattac
     5161 tataacgcga cttcggctgg caagactttt ttacgtcaca aaaacctccc cagaatcggc
     5221 cgcaatttcc ccaccgtagc ggctggccaa agttttcgct ccgttaccag cgcagcgtct
     5281 agcgcgaagt gctccggcca attcggctca cgcgggatac gcggcataag atcgacaatt
     5341 acccttttcg ccgtctcggc gtttaccaac aaatgttcga agacagtttg cgccgacacc
     5401 ggctcctcct cgattttcca gcagtcataa tcggtgatca tcgccatggt ggcgagtgcg
     5461 atctctgcct cgcgcgcgag tttcgcttcc ggcaaattcg tcatcccgat gacatcaaag
     5521 ccgtggcgcc ggttgagttc ggattcggcg cgggtggaaa acgccggccc gtccatgttc
     5581 acgtaggtgc cgccgttatg cacggtgatg ccgagcgacc gcgctgattc cgcgacgaga
     5641 ttgcgcaagc tgctgctgat cgggtcggcg aacgacacgt gggcggcaat gccattgccg
     5701 aaaaacgtgt gggcgtggcg gaaactcgtt cggtcataaa attgcgaggg caacaacaca
     5761 tcccgcggcg cgtatttttc ctgcaaactg ccgacggcag tgacgcagac gatccagcgc
     5821 acgcctaacg aacgcaacgc ccacacgttc gcgcgatggt tcaactcgtg cggcagaatg
     5881 cggtggcccc ttgcgtggcg cgacagaaaa taaacctgtc gtccgctgat cgtgccgcta
     5941 atcaacgcgt ccgagggcgg cccgaacgga gtgtcgattt tgtgctcggt cggatcccgc
     6001 agctcctcca tctgatacaa gccgctgcca ccgataatcc cgattgctgg agcctcatct
     6061 tccatcgctg ccgtgattta atctgaagct ttgcagaatt ggaaagtcga taatggcgga
     6121 tgctgttcgg ccgtgtattg ttctcgcccg cgatgccgaa atttttcatc aagacgtacg
     6181 gttgccagat gaacgaacgg gattcggaac aggtcgcgca ttcgcttctc agccgcggct
     6241 atgaacgcgt tcatgatgag agccaagccg acgtcgtgct gctgaacacg tgtagcgtgc
     6301 gcgatatggc cgagcaaaag gcgctcggga aaatgggcat gctcggccgt gtcgcgaagg
     6361 aacggccgca cgttgttttc gggtttctcg gctgcatggc gcaggcccgc ggcgcgtcgc
     6421 tgctgcgaaa tctcccgcat gtcgatcttg tcgttggcac acaaaagttc catcgcgtcg
     6481 ctgactacgt ggaggatctc gttctccaaa aacgcgctct tgtagccggg gtcgccgacc
     6541 ccggctacag aagaatcgac gacttgcgct tttccattgt cgatgtggca gacgaacccg
     6601 gctcgcaatc gacgattcgg gaacaacaac tcgcaccgaa acaggcgacg gcctttgtct
     6661 cgatcatgca gggttgcaac atgcattgca ccttttgcat cgtgccgcag acccgcggag
     6721 ccgagcgcag ccgaacaatt gaagaaatcg tcgacgaagt tcgcgatctc gtcgcacgcg
     6781 gcgtcaaaga agtcacttta cttgggcaga tcgtaaatct ttatggccgc catgaattcc
     6841 cgaaggtcga caaccaaagt ccattcgtgc agctgctcga agcggtgcac gacacccagg
     6901 ggcttgagcg gctccgcttt acctcaccgc atccgatcgg gtttcgcgat gatttgatcg
     6961 atgcgctcgc gcggttacca aagctgatgc cgcacgtgca tctgccgctg caatccggat
     7021 cgaaccgcat cctgaaagcg atgcaccgca cttatacctc ggagaaatat gtcgatcttg
     7081 ttgaacgcat tcggcgggcg cgccccggaa tttcgattac cactgacgtc atcgttggat
     7141 ttcccggcga aagtgacagt gattacgagc agacgcgagg gttggtcgaa agaatcgaat
     7201 tcgacaatgc attcgtcttt cgctattcgc cgcgacgcga cacaccagcg gcggcaatgc
     7261 caaatcaggt ggatgagctg atcaaagagc agcgcaatca ggatctgctc acgatcgtga
     7321 atgcatcggc ccgccgggct ggcgagcgat tgattggcca agaagttgaa gttctctgcg
     7381 aagggcggag caaaacgaac tcggcgcggc tgatgggccg tacgcctacg aacaagatcg
     7441 tcgtgttcga aggcggcaac gagctcgtcg gcggacttgt cgatgttcgc gtcgagcgcg
     7501 cgaccggctt cagtttgtac ggaacaccgc gggtgagctg cgccacgctc gcggcatgat
     7561 ttaccatctg tcgttgcgcg cggcgggagg cgcggttggc gcggcgttgg tattgctcag
     7621 tctgcccggt ctgttcaagc ctgacttggc cgcggcggtg caaaaattcc cgcgctcgcg
     7681 actcgccgga ttcattgtgc tgacggtcga tcttgtctgg acgatgtggc tactggcgac
     7741 gatcgaaatg ggcgagttct cgagctttcg ccgcccgctg ctgatcgcgg tgccgatcgg
     7801 ttatgttctg gcgcttcgtt ttgtggacga attcctcgcc gtgcgcgcgc tcgggatttt
     7861 ttgtttgctt ggcgccgagc ctctgctcga tgcggcattt ttgcgctacg aattgagccg
     7921 gctgctggtg acagtttttg cctacgcgtt ggtggtcgtg ggcctgttct gggtcatgat
     7981 gccctattta ctgcgtgatc aaatcaattg gagtgcgcag agcacgccac ggtggcgaac
     8041 cgtgcatgcg gtctgtttcg tctatggcgc ggcgattctc gcgtttgcgt ttacgcaata
     8101 ttgaagcgga tcctcgtcat ccgcggcggc gcgattggtg atttcgtgct gacgcttcca
     8161 gcgctgcgtg cattgcggga agcgtactca gaagcatatc tggaaattct cggctaccaa
     8221 cagatcgcca cgctggccgc catagccagc tacgccgatg cggtccgttc catcgaatac
     8281 ggcccgctct ccagtttctt tgcgcgaaat gcagagttgc cgggagaact ggcggcgcac
     8341 tttaacagct tcgatttaat tctgagctat ctctacgatc cggaccggat ctttgaaagc
     8401 aatttgcggc gcgccggcgt tgagaatctg atcatcgggc cgggcaagct cgcaaactgc
     8461 gagcacgcgg ctcagcaact ggcgcgcccg ttgcaggagc tcgggattta cgtcagcaaa
     8521 tttgcggcgc agatttcgcc ttccatccaa gatcgacatc ttgcatcgga gttccttcac
     8581 gcctgcgttg aaccgattgt cgttctgcat cccggcagcg gcagtgagaa gaaaaactgg
     8641 ccgctcgcca attggatcga gcttgggaac gatttgttag gcgcagagag cttcggcggc
     8701 tcgattgtga ttgttgctgg cgaagccgac gcggcgcagg tcggccaact agagacgacc
     8761 tggcaaaccg ggcgtgttcg cttcgcgaaa aatctgccgc tgcctcttct cgctgcgatt
     8821 ctggaccaga cggtctttat cggccacgac agcgggatat cgcatctggc cgcggcagcc
     8881 ggcgcgcatt gcattttgct tttcggtccg accgacccaa aagtctgggc gccgcggaat
     8941 gacaatgtac gcttgatcca ggcgccggat ggcgatttgc gagcgctaga tgtcgatctt
     9001 gtgcgcgcgg agcttgttca agagctgatg cgcatcggca tcagcacata aagaaacggc
     9061 gtctgaattt tcagcacgcc ggggctcatc tcgtcgatca gatcgagata aacttcgtcc
     9121 tcggtgaggg cccgcaacgg cgccatcagg aattccggat taaaggcaat cgagaaatcg
     9181 cgtcctttgt aggcgacagg cagcgattcc ttcgcctcgc ccacttcggg cgtgttcgct
     9241 gtaatctcga tgttgttctt gctgaagttg agcttgatcg aattcgattt gtcgctggcg
     9301 agcaatgaca cgcggcgcaa cgaattcaaa aaggtctcgc gctcgagcgt gacgcgctcc
     9361 ttcgcttcgg tcggaatcac ctgtttgtaa ttcggataat ttccctcgat caattttgag
     9421 acaaggagcg tgttattgac gtcaaaggcg atctggccgc tgcccatgct tacttttacg
     9481 tcgccattat cggtcagcag acgctgcaat tcggttaccg ctttcgttgg gacgatgatg
     9541 tccgcttcat ggctgcgcgg aaactcgagt tcaatgtcga ccatggcgag ccggcggccg
     9601 tccgtcgcga ccagcgtgag tttgttttct ttgaagctaa acaaaacacc gttaagcacg
     9661 taacgcgttt catcggtcga gatcgcatag gacgttttgc gcaaaccgtc gcgcaaatcc
     9721 ttttgccgca tcgtgacaac cttcgcgtct tcaaacttcg gcagcggcgg aaattcctcc
     9781 tccggcaaac cgagaatttt gaaaaaactt tgcccgctcc ggatcgaagc cgtgtttttg
     9841 ccatcgacat cgaagtgaat ttcgctcgac ggcagctcgc gaatgatgtt gaagagccgc
     9901 cgcgccggca gcgtggtcgc gcccgatttt tccacgtctg cttcgaaact gccgcgcacg
     9961 ccgacgtcga gatcggtggt cgtgagatgc acttctttac cgttcgcctg caacagcacg
    10021 ttggataaaa ttggtaaggt tgtgcgagtg ctgacgacgt tttgcacctg ttgcaaaccc
    10081 tcgagcaatt tctctttcgt aaccgtgaac ttcatggccg cagcggcgct taagatgtaa
    10141 ggcttagggt tttattcggc aaccgcaaac tctgctcaac gctgcagcga actgtcgatg
    10201 aaggaaatcg tttggcgcaa actgtcctgc tctttcatgc gtttcttcac cagcttgcac
    10261 gcatgcaaca ccgtaccgtg atcgcgcccg ccgaaggcat cgccgatttc gttgagtgag
    10321 gctttggtca gctcgcgtgc gagatacatc gccacctggc gtggaaaggc aatattggcc
    10381 ggccgccgtt tgctcgtcat atcggcgaga cgcacgtcga aatgctcggc cacgcgccgc
    10441 tgaatttgct caatcgtgat cgcgtgccgc gcttcctcct gcaaaatgtc cttgagcaaa
    10501 tgttccacca catcgtgggt cagttccttg ccgctgagtg aggcgaacga cgccacccgc
    10561 atgagcgcgc cttccagccg gcgcacgttg ctgcgaatgc ggttggccag gaactgaaaa
    10621 atgtcatcgc tgagcttgat ttgcatggcg cgcgctttca tgcgcagaat cgccatccgt
    10681 gtttcgatgt cgggcggttg aagctccgcc gtcaagcccc actcgaagcg cgagaccaat
    10741 cgtttctcca gattcgcgat ttccgacgca ggtcgatcgc tcgagagcac gatctgtttg
    10801 tgaccgtcaa agagtgtatt gaacgtgtgg aagaattctt cctgggatcg ttccttgccg
    10861 gcgagaaact ggatgtcgtc gatcaaaagc acatcggcct gtcggtaacg tttgcggaat
    10921 ttgacgagat tgctgtgctg aatcgcgtcg atgaactcgt tgatgaaaag ctcgctcgaa
    10981 acgtaaatca ccttcgtgct tttctttttt cgccagacga aatcgccggt cgcctgcatc
    11041 aggtgcgtct tgccgaggcc gacgccgcca taaatgaaga gcggattgta tgtccgggcc
    11101 ggtgcttggg caacggcgag gctcgcggca tgcgcgatct cattgtttgg accgacgacg
    11161 aatgtctcaa acctgtttcg tggattgaga ccgagggcgc tcccactgct tttcgtctcc
    11221 tgcgacgttt ccgggcgctg cgacacgatc gcgtcctccg ccacctcgcc ggagttgccc
    11281 ggaaaaacaa actttacttc gcgcggccca ccgaacgcgg tcatgatcgc cgcctgcaat
    11341 gcagccatat gattactctc aatccagaat tgataaatat tgtttggaac acgaaacgtc
    11401 agtcgcgtgt ccgtggcctc ggctaattcg acggcagaaa accagcgctt gaaagtatcg
    11461 gcgctgacct gtggtttgag ggcggcggcc agccgctgcc aaaactcagc gcatttgtct
    11521 ttcataagag ttgttaacag tgttacacaa ccgtcacaga gccatttttc gagggaaaac
    11581 gaccgatgcg gtggacgccg gccgcctttc cgccggtaat atggcacctg ggcagtgttg
    11641 ctgaaaaaag gaaaaacttc tgaaatacga gggaaaaact ggttttgtga cagcggcgcg
    11701 tcgcgtgcgc tgcaccagag cgacatcttg ggcccggtaa cttagagatg aggttaaggc
    11761 atcactcatg taactgcttc gcgacggtca aaagctgttc acaggttatt cacatgcgct
    11821 cctttttcgc cctgccgcac ggctggagat tagaaatcgc gtttcggagc gacaagaaaa
    11881 aaaccgaatt ttattttttt tcccgcgcaa aaaaatcttg acggaaaaat caaaaagagc
    11941 gtgtgcccga aaacgccctc ggcgctccct cgtataaaat tgcaccgagt tgacccgact
    12001 gctgcgggcg agcatttttc tgccgtttgg aaactatggc ggagtcaatt cgcgcgccga
    12061 agaatgaagt tgcagcagaa ccagatcgcg aattgccgtc gaaaactcct tacccgcgct
    12121 gatattcccg tggtcttgag cacgcgcact ggtcttgctc tttcgcttgc cgtcttcttc
    12181 gcgctctcgg gcataaaaac atccgcgatt gaagaattgt tgccaccatt cggctttcgc
    12241 tggaacgacc cggtgtcgcg cgtcgaggcg gtgctcgagg gcgccaaggc gcgcatcgtt
    12301 tcccgcgaga aaaaggaagg gcgcgaggtt tggacggtgg aaggcctcgt tcatccgggg
    12361 ctgaagcgga cgctcttcac cttcaagggc ggtgcgcttt tcggcgtcga gttgcagtac
    12421 gaatatcccg attggtcgat cgagcgctac aaccagcgca tgggcgaaat ccgcaagtac
    12481 ttcgacgaca aatacggcac cgggaaatta gtttcacgcg cgcgcgatac agacagtgac
    12541 gtcgtccaga cgctggttgg ctatcaatgg atggtcggcg cgaccatgct ggagctgttc
    12601 tatttttcgg cgcagcacga cgcgcttgtc taccgcacga tcacggtcga ttacaaagcg
    12661 ctgtgattct gtaggcgcgg cgctatgcgc cgtctcgccg caatcacaac acacgagccg
    12721 caggcgcgtg cctacagcac cgtgtcgctc ggctgcaatt gtcgatcttg cgcccgcacg
    12781 cgctggccgc gcttagtctc tttctgcaga cgtttgaggc ggcttgccaa cactgctttg
    12841 gccgcagagt tcatccgatc aatgaacagg actccgttta gatgatcgat ttcgtgctgc
    12901 agcgcgcgcg cgagcaaacc gcccgcttcg aattcgattt ctccatccaa tgtttgcgcg
    12961 cgcgcgatta ctgagccggc tcgctcgatc tcgccggtga tttccgggaa gctcaaacag
    13021 ccttccagac cgagttcagt ttcgccgcgc aactctaact cgggattaat cagtaccagc
    13081 ggcatcgcgc tctttggatc gacctcttcg ccgttaattc gcatcgtgct gggccggtcc
    13141 tccgcttgcg acacatcgat cacggttaat tgcaatgctt cgccgatttg ttgcgcggct
    13201 aaaccaatgc cattggccgc gtgcatcgtc tcgagcatgt tcgtcgccag ctcgcgaatg
    13261 tgattgtcgg tcttctcgat gcgccggcct ttggcgcgca gcatcggatc gccgtaacgc
    13321 aagatcgaca atttcattgc ggctcgcgtg taaacgacgg gagatttttc acccccgcca
    13381 gtttgagcgc atccatcact ttcacgatcg tgccccagga cgccttgcga tcggcctgca
    13441 atgccagtgt cgatttgcgc gtctccggca attcacgcac cgcagcttcc aattgatcca
    13501 cgtccaccgc gcgaccgtcg agctgaatct gatcgctctc atccacgctt acgattacgt
    13561 gctcgagctc ggccggtttc ttcgtcgccg tttttgattc cgggaggtta atctgcacct
    13621 ccggctggtc ttttttgaac gtggtcgaaa cgacaaagaa gatcaggagg atgatgagaa
    13681 tgtcgacgag cgagacgatg ataattgtcg gtgcgcgcct ttttcggact gcgaagttca
    13741 ttcgctaaac tcgcccgcgc ctctcacgcg accggagtgg ggacggtcac agtcggcggc
    13801 ggcgaagttt tcgtgggcac gaattcgcgc gagcttcgcc cgtaataaca tttgttgatc
    13861 aattccacga ccagcgtctc catctcgact gacatcactt cgactttttt cgaaaagtag
    13921 ctgaacccga tcagcgtcgg caccgcgatc gaaagcccga acacggtcgc gttcagcgct
    13981 tcggcgatgc cgagcgcgat ctgccgtgtg tccgatgcgc cactgctcaa gccgagatgt
    14041 gagaaaacat gcaccagacc ggagacggcg ccgatcaatc ctagcagcgg cgcgataccg
    14101 acgatgacct ccagcacgat caggcccttt tccagccgca ccatttcgtg gcgcgcacgc
    14161 gtctccacca cctcgatgtt ttcggagcgc ggcgcgcgca aatgctgcaa tgcgactcgc
    14221 gcaatccgcg cgagtgacga tggatcatga gccacgatcc gggccagccg ttcggcactc
    14281 gcgccgggca ctagccgctc gatctcgctc tgaatcaatg atggcatcac gtttttctct
    14341 cgcagcgcaa ggccacgcaa aatcattgtc gtcaccgaca caatcgagca ggcgagcagc
    14401 ggccacatga agagtccgcc cttggtgaaa aagccgagga ccgtttgtgt gatttccgac
    14461 atatcagcca gtgccacagc ggcgctcaat tagcatacat cgtaaaagac atttcttcgt
    14521 ccaatccttc cggcgggacc gtgctggcta cgtcatcggg cagcggcggc agctcaatct
    14581 cgagcaccga ttgcaagcaa acattcgcga atgattcgtt ggagctattg gccacaacct
    14641 tcagattctt cggcttccca tcgcggccaa ccgaaaaaac cagacggagc gtgccgatgc
    14701 tgaccaagtc gcgctgtttg tttacgtaat aataccaacg cgaaccgacc gcatcgtaaa
    14761 ggagtttctg atagcggccg agcggtgtgc cgactgcgtt gaccgaaggc gcgccgcgat
    14821 tggtgatcga cccggccaaa cgcgtctgct gtttctccgc gcgatatgtg gaggcgggtt
    14881 gctgcggagt cggagcgacg ctcggctgca cggtcggatt cggcgtcgac gttagcatcg
    14941 caaactgctc cgactcggaa agcggcgttg gctgcggagt agtttccggc gcctgcgacg
    15001 gctgcggcgt ttcgttaggt actgcgctcg gttgcggctg cacaccctga ttggcgagcg
    15061 aatattgatg tgtctccaaa tcgacggccg gtcgctcctt tccttcctgt gaaggtactg
    15121 gcagatcgcc cgtcgctgga agctggcttg cgccgatcga attcgcgttt gactcgaacg
    15181 ttttttcctg cggcacggga gcgttcttgg attcatcggt ttcgatgaag gccgggttct
    15241 tcggaactgt cgcgggtgca aggtcgacaa tcgtcagctc agccggttta tcttcaattg
    15301 acggcgccga agaaaaagcg ccgccgactg ccgccagtcc gaacgcgacg agcagatgaa
    15361 tgagcagcgc cgcgacaaac gcccagaaaa ctttgcggaa ctcgtgtttc cgatccagca
    15421 acgtgtcgcc cggctgggca aacatcatct cggcagtcgt cat
//
LOCUS       DASTDG010000205        12862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_759953, whole genome shotgun sequence.
ACCESSION   DASTDG010000205 DASTDG010000000
VERSION     DASTDG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759953"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFO30_06655"
     CDS             complement(<1..1115)
                     /locus_tag="VFO30_06655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9419005.1"
                     /translation="MTAHPVSIVKVRSRAERDEFIKFAWRIYKNDPSWVPPLIIERRA
                     FLDRKTHPFYQHGDAALFLARRNGQVVGRVMASDDPNYNALHRTNVGCFGLFESIDDV
                     DVAAALFRAVESWLVAKGRNEIMGPIDYSTNYVCGLLIDGFQHPPTVLTAHNPTYYRR
                     LIELCGFEKIKDWYAWWFVPALEPLQRLHRIANARARKHVVKVRQIDLRRLTHESRRL
                     SAVFNEAWKNNWGFVPFTEPEAEHMAKEMRRLIDPRMTLIAEIDDAPVAFVICVPDIN
                     VALRRINGRLTRFALPIGLLQLLYYRRRIRTARFVALGVLEKYRRAGIAEMLVLRVME
                     EGAKRGFTAELSMTLEDNYMVNRFIEALGARKYKTYR"
     gene            complement(1112..1981)
                     /locus_tag="VFO30_06660"
     CDS             complement(1112..1981)
                     /locus_tag="VFO30_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9419006.1"
                     /translation="MEGIVDLWFYRRIGFRLAQFFVRRNWTPTSITLIGGIFGIVAGH
                     LYFYRALVLNLLGLVFHVAANIFDNADGQLARLTNQQSRIGRILDPVVDHIIWFSIYL
                     HLALRLQFEGFSSAIWALALAAGLSHAAQAAAADYSRNAYLYFGKGRPEFDSASNLKR
                     EYRRYTWRANLWRKFLLGLYLNVTREQELLLPGVKQLHERVDHDLAGQIPVRFQSRYG
                     DLVLPSLKWWGLLMTNTRMLVLFAVFLVRQPVWYFWFEITAANFLLFYLVRKQEKISR
                     SLVELLTTQKESA"
     gene            complement(2064..3314)
                     /locus_tag="VFO30_06665"
     CDS             complement(2064..3314)
                     /locus_tag="VFO30_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9419007.1"
                     /translation="MSDPAKLSLSNFLAGESRDPLQAPPSFTAWMQAGAWAVELYEPE
                     MLASAEARTSIRYDGKAQRVINLCSYNYLGLANHPDVIVAAHHALNTHGLGACGSPML
                     SGMTDLHRELESRIAKFLGREDAMLFNSGFGGALGTISGLLRKSDVAILDNRSHLSLR
                     DGAVLSRCRTEKFEHNDAASLDAALARQTGRRQLVIVEGIYSMDGDFGKLAELIDVAE
                     SHGASVFIDEAHSVLGCGNHGRGAAERFGVEDRVSLIYGTFSKGFGALGGFVAGSKET
                     LQYLRFYAHPYVYSCALPPVVIAGLLQALEIAIREGQKLRKALWENADYFHEKLRELG
                     IDTGSSTTYVMPIVMGDRERMYRLCHELRRRGLWVAPVDYPAVPQNRICFRACVTAKH
                     TRADLDEALNILQDTLVPAALQNA"
     gene            complement(3419..4033)
                     /locus_tag="VFO30_06670"
     CDS             complement(3419..4033)
                     /locus_tag="VFO30_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9419008.1"
                     /translation="MASRLYVSNKNETVRMFKSDVMEFLSHVHPATPLVLYLPLVAYM
                     LYAAIWKSGLSMLAVAGLFAVGLVIWTLLEYIIHRWVFHYEPKTPIGKKLHFIVHGVH
                     HDYPNDGRRLVMPPAVSMPLAVIFWLLFAVTLGRFAPAVSAGFGFGYICYDSIHYAIH
                     HFPMKRGVWLWLKQYHLRHHYHDDHTGFGVSSPLWDYVFRTNRR"
     gene            complement(4355..5656)
                     /locus_tag="VFO30_06675"
     CDS             complement(4355..5656)
                     /locus_tag="VFO30_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET9419009.1"
                     /translation="MNVKRVLLSLWLLIAAFAAHAQTLTHGPVVGGVTDTQATIFVRT
                     GVPAKVTIRYSTDLGLLMYSESRTIATTTSQDNTATVPISGLKGGTKYYLDVAVDGVA
                     QLRRPYPSFTTFAPAGAARGFKFVVLTDFATVNSLQRSSFTWQSAAAEQPDFVFIGGD
                     FDHRNPKTLGAKRVMFKDLYNPSTPNMDGFVTFILRQFPIAHQWDDHDSGLNNLDTTY
                     PDWNLTQQAFHEYTPSYPLPKISPAGIWQKFSYAQVDFFVLDCRSQRTPETARDDSKK
                     TMLGAVQLQWLENGLLASTATWKIIFTSVVTNPTTKYPDGWAGYQTEWRALRDFIRGN
                     KISNVVFIAGDLHLGAIDNGAASGFPEMCVPQANGAEDGLHCPTSAYGIWSEGYSTSD
                     CSGYSIVTVRQDPDRLLLETADEFGSRTISYSLPSANSVFR"
     gene            complement(5853..5925)
                     /locus_tag="VFO30_06680"
     tRNA            complement(5853..5925)
                     /locus_tag="VFO30_06680"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5889..5891),aa:Glu,seq:ttc)
     gene            complement(5986..7278)
                     /locus_tag="VFO30_06685"
     CDS             complement(5986..7278)
                     /locus_tag="VFO30_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET9419010.1"
                     /translation="MIRFLYNLLWPLGFIFFLPGYLAKMFRRGDYRANFGQRLGIYDV
                     DVVRRLTNRKPIWVHAVSVGEVGIASKLIGAVRELNANAEFVLTTTTTTGFAVATNNA
                     PAWIEVLYAPLDFWPIMRRALRVIRPRAIVLVEAEVWPNLLAEAHARRIPVALINARL
                     SPRSEKRFRRFGFFVRPTFRLLDLLLVPEPADVERWEALGIPPGRIHPVGSVKYDPQN
                     ATIDATVPHTVLHELKIDNRPILLGGSTHPGEEEFVAQVFRALRRDFPELFLILAPRH
                     AERGRAVARLLEQMELRVVLRSRASATDHPADCLVLDSTGELRNWYAVATIVFIGKSL
                     LAHGGQNPAEAILARKPVVFGPHMENFAAFARALVARGGAIQIRSAEELRGTIVDLLR
                     DTAARERRVTNASQVLAAHTGATARTAKLIIDLNRAEE"
     gene            complement(7275..7823)
                     /gene="rsmD"
                     /locus_tag="VFO30_06690"
     CDS             complement(7275..7823)
                     /gene="rsmD"
                     /locus_tag="VFO30_06690"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET9419011.1"
                     /translation="MRVVGGSAGGIQLAVPKRGVRPTMDRVKAAIFSSLRDRTPGARV
                     LDLFAGAGALGIEALSRGAASALLVDQDRQAILAIKKNLAKTKLDGRLRQQEVFRFLL
                     EAGAREKFDIIFADPPYDKMETGESFIEKLLSNKQLGELLDADGIFVLEKRPDENLPD
                     IKPWHIVRAKTYGATEVLFLQK"
     gene            complement(7820..9334)
                     /locus_tag="VFO30_06695"
     CDS             complement(7820..9334)
                     /locus_tag="VFO30_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET9419012.1"
                     /translation="MRRLVLFGFGAIVALAAALLIALNLYVQSEGAQAWIEAELSERV
                     GTALHIRGTGVTPWGGLTLSGITIPQRSTLTGVDFLQAKSFHLHLRLLPLFSHRLVIT
                     SIALIDPTVVWPQNAEGKWRLPSAAPQPLTPPPAQMPSIASSPAEAVSPTPPQERYVS
                     PAGKPRRMKSALAVEIRRLNISGGNFQFLDRTGLPLATFENLKFSSTLSDAHTLRGSA
                     KVAKISLRNRFFVAGFHSPVRFEQNVLELPKISGQIGSGELNAKFKLDVQSAGSPFSV
                     SANLRNVHADQIVAEAGGAKNLLQGKLDAALEAAGKTADPNALTGHGQISLRDGQLQQ
                     YSLLVALGQILQIDELSQLHLDQAWATYHLTPGRVNIDELVLRSPNIRLSATGTLGFD
                     SELRMDSQLAISDQIRGRLFKPLRDNFQPIDEPGYSAIAFNVAGTIDRPSTNLLDRMV
                     GRELKDFVRGLFGSKKSKKKKAPENEPAEVALPTAAPSVPADVTSPAATSTPSP"
     gene            9398..10267
                     /locus_tag="VFO30_06700"
     CDS             9398..10267
                     /locus_tag="VFO30_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9419013.1"
                     /translation="MNKTVVILNPAAHSERAVRLRPQVESIARDCLVTTTGRPGEAEA
                     LARHAVSEGFEKIVAAGGDGTINEVVNGLAGSSAILGLLPIGTVNVFATELGLPVHDL
                     EMCWHIIRGDQTRIIDLPSANGKHFVQLAGVGLDAQVVKETSLAFKRSFGPLSYLISA
                     AQIAARTPPRLFIDAENAPIRESSFVLIGNGRHYGGPFPFFKHAVLDDGLLDVVAFRR
                     LGYLEIVKYLQDVFFSSDIRLPEVEYFQTERLRITSDRDVPVELDGELVGSCPVEFET
                     REKRLRVLAPPPS"
     gene            10307..11146
                     /locus_tag="VFO30_06705"
     CDS             10307..11146
                     /locus_tag="VFO30_06705"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9419014.1"
                     /translation="MRLSGGKRSFYTRRLRERQQVVLPLLIGVNAIIFVAQLVVDLYQ
                     PGFVREYLGISNHGVSDAYAWQFLTAMFLYSGPWHFAANIFLLYLLGRDLESIVGSRH
                     FFYLYFAGLSAGELGHLFLMPPNSMLLASSGGVTALIVAYALVLPELELLPSGSLFRS
                     LRLKAKHLAYATIALALIFACVFRSGTLTHSAWLGGCAAGWLYAHLLGFGRPSFLQRF
                     LQHRREQVHRYDRMTVEELIAQEIDPLLDKIGHSGFGKLSRRERRELLRAHAKLLEKQ
                     EVP"
     gene            11260..>12862
                     /locus_tag="VFO30_06710"
     CDS             11260..>12862
                     /locus_tag="VFO30_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HET9419015.1"
                     /translation="MDSVDRWIEQKAVTTFHPAQVEASLRSLHDAWPAEAPPLARMIE
                     QFPLGEAALLHLFAASSICAARSVRDPTILLWLAEPKICGSQRGYAEMSEALHAMSRN
                     GLAANHFRALRQWKAREMVRIALREVANAAALEETTAELSQLAEICIRCVYEHCNAKL
                     RRRHGSPQAEFAILALGKLGGRELNHSSDVDLLFVYSADGELVPGRSYHEFFNELAEQ
                     ILETFSRPHPEGPLLRVDVRLRPEGPAGPLARSVDSMEHYYGGFGETWERLALIKARH
                     VAGSREVGYDFLRQLQTFIYPKSATPDLLEEIAGIKHRIERDVVGERKLGRNVKLGRG
                     GIREIEFIVQTLQFVHGARHTFLQEPNTLKALRALAQLELLPRSEIFDLDRAYRFLRR
                     VEHRLQIEAEEQTHTIPADPERLRRLARSLEFESADKFQTALEREMTAVRCLFRKVIA
                     EAPGSQETGALNLDIFSERAAAERAMADLMHGAGAAHTSKRRRQSARRLQPVLLGQLA
                     KTADRDAALTKFVRFVEAHGMRSLLF"
BASE COUNT         2813 a         3666 c         3734 g         2649 t
ORIGIN      
        1 atccggtagg ttttatattt ccgggctccc aacgcttcga tgaaacggtt aaccatgtaa
       61 ttgtcttcaa gcgtcatact gagttcggcc gtgaaaccgc gttttgctcc ctcctccatt
      121 actcgcagca ccagcatctc ggcaattccg gcgcgccgat acttttccaa aacgccaagg
      181 gcgacaaagc gcgccgtgcg aattcgccgc cgataataaa gcagctgcag taatccgatt
      241 gggagagcaa aacgcgttaa gcgtccgttg atgcggcgca gcgcgacgtt aatgtccggc
      301 acgcaaatca cgaacgcgac aggcgcatcg tcgatctcgg cgattaatgt cattcgcgga
      361 tcgataagac ggcgcatttc ttttgccata tgttccgcct ccggttccgt gaacggaacg
      421 aaaccccagt tatttttcca ggcttcatta aacacggcag agaggcggcg actttcgtga
      481 gtcaagcggc gaagatcaat ctggcgaacc ttgaccacgt gctttcgggc acgtgcgttc
      541 gcgattcggt gcaagcgttg aagcggttcc agcgcgggga caaaccacca ggcgtaccaa
      601 tccttgattt tttcaaaacc gcaaagctcg atcaatcgcc ggtagtaagt tgggttatgg
      661 gcggtcagga ctgtgggcgg atgttgaaaa ccatcaatga gcaatccgca aacgtaattg
      721 gtcgaataat cgatcggccc catgatttcg ttccggcctt ttgccacgag ccagctttcg
      781 accgcccgaa atagcgcggc cgcaacgtcg acatcgtcga tcgattcaaa taaaccgaaa
      841 caacccacat tcgtgcggtg caatgcgttg tagttcgggt cgtcgctcgc catgacgcgg
      901 ccaaccactt ggccatttcg tctcgccaga aacaacgccg catccccgtg ctgataaaac
      961 ggatgtgttt tacgatcgag aaacgctctt cgttcgataa tgagcggcgg cacccaggac
     1021 ggatcatttt tataaatgcg ccacgcaaat ttaatgaact catcccgctc ggcgcgggag
     1081 cgaaccttga cgattgagac gggatgagcg gtcacgcgct ttctttttgc gtcgtgagta
     1141 attcaacgag cgagcgcgat attttttcct gtttccgcac cagatagaag agcaagaaat
     1201 tggctgcggt aatttcgaac caaaagtacc aaactggttg tcgaactaaa aatacagcga
     1261 acaggaccag cattcgggta ttggtcatga gcagtcccca ccatttcaag ctcggcagaa
     1321 caagatcgcc gtagcgcgat tggaatcgga ccggaatttg cccggcgagg tcgtggtcga
     1381 ctctttcgtg caactgtttt acgccaggca gaagaagttc ctgttcacgg gtgacattga
     1441 gatacaaacc gaggagaaat ttccgccaca agttcgctct ccaagtgtag cgacgatatt
     1501 cccgcttcag gttcgacgcc gaatcgaatt caggtcgacc ttttccgaag taaagatatg
     1561 cgtttcgcga gtaatcagct gcagctgcct gggcggcgtg actgagacca gctgcgagcg
     1621 ccaatgccca gattgcgctg gaaaaacctt cgaactgaag ccgcaaggcg agatgaagat
     1681 aaatgctgaa ccaaataata tgatcgacaa ccggatcgag aatccgtccg atgcggcttt
     1741 gttggttcgt taggcgagca agttggccat ccgcattatc gaaaatgttc gccgccacgt
     1801 ggaaaaccaa ccccaggaga ttgaggacaa gagcgcgata gaaataaaga tgaccggcaa
     1861 cgatcccaaa gattccgcca atgagagtaa tcgacgtcgg cgtccaattc cgccgcacga
     1921 aaaattgcgc caaccgaaag ccaattctgc gataaaacca aagatcgaca attccttcca
     1981 cctcgcgaga tttgtaggtc gcgtggatag ccgacgatga cggctttgca ggctcacaga
     2041 ggcggctaag ttctgttccc acttcaggcg ttttgcaacg ccgcgggaac gagcgtgtct
     2101 tgaagaatat ttaacgcttc gtcgaggtcg gcccgggtgt gcttggcagt gacgcaagcc
     2161 cggaagcaga ttcgattttg cgggacggcc gggtaatcga ccggcgcgac ccaaagaccg
     2221 cgacggcgca attcgtgaca aagccgatac attcgttcgc gatcgcccat gacgatcggc
     2281 atgacatagg tagtggatga gccagtatcg atgccgagct cgcgcagctt ttcgtgaaag
     2341 tagtcggcgt tttcccaaag cgctttccgc aatttctgtc cttcacggat ggcaatctcg
     2401 agcgcttgca gaagaccggc aatgacgacc ggcggcaacg cgcatgaata aacgtaggga
     2461 tgtgcataaa accggaggta ttgcagggtt tctttcgatc cggcgacaaa tcccccgagg
     2521 gcaccgaatc cttttgaaaa tgtgccgtag ataagcgaca cgcggtcttc cactccaaat
     2581 cgttcggccg caccgcgacc gtggttgcca cagcccagaa cggagtgcgc ttcgtcaatg
     2641 aaaacactgg cgccgtggga ttcggcgacg tcgatcaact cagccaactt gccgaaatcg
     2701 ccatccatcg aataaatgcc ttccacgatc acaagttggc gccgtccagt ttgccgggcg
     2761 agagcggcat caagggaagc tgcatcgttg tgctcgaatt tttcggtgcg acaacgggaa
     2821 agcacggcgc catcgcgcag gctcaagtga gagcgattat cgaggattgc gacatcactt
     2881 ttgcgcagga gaccggagat ggtccccaga gcgccgccga atccgctgtt gaagagcatt
     2941 gcatcttcac gtcccaggaa tttcgcgatg cgcgattcca gttcgcgatg aagatcggtc
     3001 atgcccgaga gcattggtga accgcatgcg ccgagaccat gggtgttgag cgcgtggtgc
     3061 gccgcaacga tcacgtcggg gtgattggcg agcccgagat aattgtaaga gcaaagatta
     3121 atgacgcgct gcgctttgcc gtcatatcgg atgctggtgc gggcctccgc actggcgagc
     3181 atttccggct catacaattc gaccgcccac gcgccggcct gcatccaagc ggtgaaactt
     3241 ggcggtgctt gcaacgggtc gcggctttcg cccgcgagga agttgctcaa actaagtttt
     3301 gcaggatcgc tcattcctta tggcaaatcg gtcgccgcgg cgacccttcc atatcggggc
     3361 agccattgta cttcgcctcg cgcagccgac aaggcctaag tgattgccaa attgcgtcct
     3421 accggcgatt cgtgcggaaa acgtaatccc acagcggcga actgacgccg aacccggtgt
     3481 gatcgtcgtg gtaatggtgc cgcaaatggt actgcttcag ccaaagccag acgccgcgtt
     3541 tcatcgggaa atggtgaatg gcataatgga tggaatcgta gcaaatgtag ccgaagccaa
     3601 acccggcaga aactgctggg gcaaaacggc ccaaggtcac ggcaaacaac aaccagaaga
     3661 tcaccgccag cggcatgctg accgccggcg gcatcaccag ccggcgcccg tcattgggat
     3721 agtcgtggtg aacgccatgt acgataaagt gcagcttttt cccaatcggc gtcttcggtt
     3781 cataatggaa aacccagcgg tggatgatgt attcgagtag tgtccagatg accagcccga
     3841 cagcaaacag ccccgccacc gctaacatcg acaatccact tttccagatc gcggcataaa
     3901 gcatgtaagc gactagcggc aagtacagca cgagcggtgt cgccggatgg acatgggaga
     3961 gaaattccat gacgtcgctt ttaaacatgc gcacggtttc gttcttgttt gagacgtaga
     4021 ggcgcgaggc cataacggtt attttagtta cctctgcgcc cggagcaacg cgttcgtttg
     4081 atctggaggc tgacgcgtcc tcggcagaat gcctcgtcag cccgggcggc agcgggagaa
     4141 aatgaccatc gaacctgatt agcccgaccc taacgggatt ccaagccgct tgccacattt
     4201 cgcgaattcc cgcgaattgg gcggaaagct gcgggaatta gcgcgttact aaccgatcac
     4261 gagaatctaa gaattgcact attcgcgcag aattatgcag ttcgactgtc cgaatgtgtc
     4321 cgaaacgcct cgcgcctgag agacacagcg ggaatcaccg aaaaacagaa tttgcagagg
     4381 gcaaactgta agaaatggtt cggctgccaa actcgtcagc cgtttccagc agaagccggt
     4441 ccggatcctg ccggacggtg acgatgctgt agccggaaca atcgctggtg gagtaacctt
     4501 cgctccaaat cccataagcg ctggttgggc agtgcagtcc gtcttcggcc ccattcgcct
     4561 gagggacgca catttcgggg aagcccgagg cggctccgtt atcgattgcg ccaagatgca
     4621 aatcgcctgc gataaagacg acgttgctga tcttgttgcc acggatgaaa tccctgagcg
     4681 ctcgccattc cgtctggtaa ccggcccagc cgtcagggta tttcgtggtc gggttggtga
     4741 cgacggaggt aaaaatgatt ttccacgtgg cggtcgaagc gagcaggccg ttctcgagcc
     4801 actgcaattg aaccgcgccc agcatcgtct tcttgctatc atcgcgcgcc gtctcgggag
     4861 tgcgctggct ccggcagtcg agcacgaaga agtcgacctg ggcataggaa aacttttgcc
     4921 agatgccagc gggtgaaatc ttcggaagtg gatagctggg tgtgtattca tggaacgcct
     4981 gctgcgtgag attccagtcg ggataggtgg tgtcgagatt gttcaggcca gaatcgtgat
     5041 catcccattg gtgcgcgatt gggaactgtc gcagaatgaa tgtcacgaag ccgtccatgt
     5101 tcggcgtgct ggggttgtat aggtctttga acatcacgcg tttggcccca agcgtcttcg
     5161 ggttgcgatg atcgaagtcg ccgccgataa aaacgaagtc aggctgctcg gccgcggcgc
     5221 tctgccaagt gaacgaagaa cgttgcaagg aattgactgt cgcgaagtcg gtgagaacga
     5281 cgaatttgaa accgcgagcc gcgccggcgg gcgcgaaagt ggtgaaggat ggatagggcc
     5341 gcctgagctg cgctacacca tccaccgcca catcgagata atatttcgtc cctcccttta
     5401 gtcctgaaat cgggactgtg gcggtgttat cctgtgaggt ggtggtagcg atcgtccggc
     5461 tctctgaata catgagcaac ccgagatcgg tggagtaacg gattgtcacc ttcgctggaa
     5521 caccggtgcg aacaaaaata gtcgcctgcg tgtcggtgac accgcccaca acgggaccgt
     5581 gcgtgagagt ctgggcgtga gcggcgaagg cagcaatgag gagccagagg ctgaggagca
     5641 cacgcttcac attcactgtt cggatttagc gcttccgata gccgccgtga aaacgccgtt
     5701 tcacgattaa aatgcaaccg ctatccggct gcgcgttttt cgcggcgaga gagaccggcc
     5761 cgatctggca ttgctgtacg agcgcgtgcc gctgctcctc agccagaacg acagctaagg
     5821 cggtggcgct tgcggtcacc tcgatctcaa agcgacccta acgggattcg aacccgtgtt
     5881 accgccgtga aagggcgatg tcctaggcct ctagacgata gggtcaaagc ggacggcaat
     5941 atcagaaccg acggttcggc gcgcaaggca aattgcccaa cgttcctatt cttccgctcg
     6001 gtttagatcg ataatcaact tggccgtacg cgccgtcgcg ccggtgtgcg ctgcgagcac
     6061 ctggctggcg tttgtaaccc ggcgctcgcg ggccgcggtg tcgcgcaaaa gatcgacaat
     6121 cgtgccgcgc aattcctcag cggaccgaat ttggatcgcg ccgccacgcg caaccaaagc
     6181 acgcgcgaac gccgcaaagt tttccatatg aggaccgaat acgaccggct ttcgcgcgag
     6241 aattgcctca gccggatttt gtccgccgtg cgcgagcaaa ctttttccga tgaaaacgat
     6301 ggtcgcaacg gcataccagt ttcgaagttc gccggtggaa tcgaggacta aacagtcggc
     6361 cgggtgatcg gtagcgctcg cgcgcgaacg caagaccacg cgcagctcca tttgctccag
     6421 cagcctcgct actgcgcgcc cgcgctccgc gtgccgcggg gcaaggatga ggaacagctc
     6481 gggaaagtcg cggcgtaacg cgcgaaaaac ctgcgctacg aactcttctt cacctggatg
     6541 cgtgctgccg ccaagcagga tcgggcgatt gtcgatcttg agctcgtgca aaacggtgtg
     6601 tgggacagtt gcatcaatag ttgcgttttg cgggtcgtat ttcacgctgc ccaccgggtg
     6661 aatgcgtccg ggtgggatgc cgagagcctc ccagcgttcc acatcggcag gctcaggaac
     6721 caaaagcaga tccagtagtc gaaaagtcgg ccgaacgaaa aatccaaaac gccgaaaccg
     6781 tttttcagac cggggagaca aacgcgcgtt aatcagcgcg accggaatac gccgtgcgtg
     6841 cgcttcggcc aggaggttgg gccaaacctc ggcctcaacc aacacgatcg cccgcggccg
     6901 aatgactctc aatgcgcggc gcatgatcgg ccagaaatcg agcggcgcgt agagcacctc
     6961 tatccaagct ggcgcgttgt ttgttgcgac ggcaaagccc gtcgtcgtag tcgtggtgag
     7021 gacaaactcg gcgtttgcgt tgagttcgcg aaccgcgcca atcagtttcg atgcgatgcc
     7081 aacctctccc acgctgacgg catgcaccca aatgggtttt cgattggtca ggcgcctgac
     7141 cacatcgaca tcatagatcc cgaggcgctg gccgaagttc gcccgataat ccccgcgccg
     7201 gaacatcttc gccaggtaac caggcagaaa gaaaatgaag ccaagcggcc agagcagatt
     7261 atagagaaaa cggatcactt ctgcagaaac aggacttccg tcgcaccata tgtttttgcc
     7321 cgaacgatgt gccacggttt gatgtccggc agattttcat cgggccgctt ttccaaaacg
     7381 aaaatcccat cggcatcgag caactcgccg agctgtttat ttgaaagaag cttctcgatg
     7441 aacgattcgc cggtttccat tttgtcatat ggcgggtcgg cgaagatgat gtcgaatttt
     7501 tcccttgcac ccgcctctag caaaaaccgg aagacttcct gctgccgtag gcgtccgtcg
     7561 agctttgtct tggcgagatt ttttttgata gcgagaattg cttgccgatc ttggtcgacg
     7621 aggagggcgg aggcggcgcc gcggctgagc gcttcaatgc ccagcgcgcc ggcgccggca
     7681 aacagatcga gcacgcgcgc gccgggagtt cgatcgcgca ggctggagaa aatcgctgct
     7741 ttcacgcgat ccatcgtcgg gcggacaccg cgtttcggca cggcgagctg aattccgccg
     7801 gcggaaccgc cgacgactct catgggctgg gtgtcgacgt cgccgcaggt gaagtaacat
     7861 cggcgggtac actaggagcg gcggtgggca gtgcgacttc ggcgggctcg ttttcaggcg
     7921 cctttttttt cttcgacttt ttgctgccga acaatccgcg cacgaaatcc ttgagctcgc
     7981 gtcccaccat tcggtcaagc aagtttgtgc tcggccggtc gattgttccg gcgacattaa
     8041 acgcgatggc cgagtagccg ggctcgtcga tcggttggaa attgtcgcgg agcggtttga
     8101 acaggcggcc gcgaatctgg tcgctgatcg caagctgcga atccatccgc agctcgctgt
     8161 cgaaaccaag cgttccagtc gcggaaagtc tgatgttcgg cgagcgcagg accagctcgt
     8221 cgatgttgac tctccctgga gtcaggtggt aagtcgccca cgcctgatca aggtgcaact
     8281 ggctgagctc gtcgatctgc agaatctggc cgagcgcgac gagcagactg tattgctgca
     8341 actggccgtc gcgcagagaa atctgaccgt ggccggtaag cgcgttcggg tcggcggttt
     8401 tgccggcggc ttccaatgct gcgtcgagct tgccctgcaa caggttcttc gcgccgccag
     8461 cctcggcgac gatttggtcc gcgtgcacgt tccgcaaatt tgcgctcacg ctgaatggcg
     8521 aacctgcgct ctgcacatcg agcttgaatt tcgcattcag ctccccgctt ccaatttgcc
     8581 cggaaatctt cggtaactcg agcacgttct gttcaaaccg cacaggtgag tggaaaccgg
     8641 cgacgaaaaa gcggttccgc aacgaaatct tcgcgacctt ggcactgccg cgcagcgtgt
     8701 gagcgtccga gagagtcgaa ctgaacttga ggttctcaaa cgtggcgagc ggaagacccg
     8761 tgcggtcgag aaactgaaag ttgccgccgc tgatgttgag ccgccgaatc tccacagcca
     8821 gcgccgattt catccgccgc ggtttaccag ctggcgagac atatctttcc tgtggcggcg
     8881 tcgggctcac agcttctgca ggcgaggacg caatggacgg catctgagct ggcggcggcg
     8941 tgagtggttg cggcgcggcg gagggcaatc gccatttccc ctctgcattc tgcggccaca
     9001 ccacagttgg atcgatcaac gctatgctcg tgatgaccag ccggtgcgag aaaagcggca
     9061 gcaaccgaag atgcaggtga aagctcttgg cctgtaaaaa atccacaccc gtcagagtgg
     9121 atctttgcgg aatcgtgatc ccgctgagag tgagcccacc ccatggcgtc acgccggttc
     9181 cgcggatgtg cagagccgtg ccgacgcgct cacttagctc tgcctcgatc cacgcttgtg
     9241 caccttcgga ttgcacatac aagttcagcg cgatcaacaa cgctgccgcg agcgcgacaa
     9301 ttgctccaaa gccaaacaac acgagccggc gcatttttgc cacacaaaga cgttacaggc
     9361 ttcaagcgaa cagcaaaacc ccggctccag atttcctgtg aataaaaccg tcgtcattct
     9421 caatcccgcc gcccacagcg aacgggcggt ccgtctgcgg ccgcaggtgg aatccatagc
     9481 gcgggattgt ctcgtaacga ccacgggacg gcccggcgaa gcggaggcgt tagcgcggca
     9541 tgcggtcagc gaaggtttcg agaaaattgt ggccgctggc ggcgacggca caatcaacga
     9601 agtggtcaat gggttggccg gcagttcagc gatccttgga ctgttgccaa tcggcacggt
     9661 taacgttttt gcgactgagc ttggtttgcc agtgcacgat ttagaaatgt gctggcacat
     9721 tatccgcgga gatcagaccc gcattatcga tcttcccagc gcgaacggaa aacatttcgt
     9781 gcaactcgcc ggcgtcggtc tggacgcgca agtggttaaa gaaaccagcc tggccttcaa
     9841 acgcagcttt ggcccgctga gctacctgat ttccgcggca caaattgctg cccgcacacc
     9901 accgcgtctg ttcatcgacg cggaaaacgc gccgattcgc gaaagctcat tcgtgctaat
     9961 cggaaacggc cggcattacg gcggcccatt tccctttttc aaacacgccg tgctcgatga
    10021 cgggttgctc gacgtcgtcg cgttcaggcg cctcggttat ctggaaatcg tcaaatacct
    10081 gcaagatgtc ttttttagtt ccgacatccg cttgcccgag gtggaatact tccagaccga
    10141 gcgactgcgt atcacgagtg accgagacgt gcccgtcgag ttagacggag aactcgtcgg
    10201 cagttgtccg gtggaattcg agacgcggga aaagcgtctt cgcgtccttg ctccgccgcc
    10261 gagctaaatc gtcgacaaat tttcccgttt gcgatagctt cgaacggtgc ggctatcggg
    10321 gggaaagcgt tccttttaca cgcgtcggtt aagagagcgc cagcaggttg ttctgccgct
    10381 gttgatcggt gtaaacgcca tcattttcgt cgcccagcta gttgtcgatc tttatcagcc
    10441 cgggtttgtc cgcgagtacc tcggtatcag caatcacggc gtgagcgacg catacgcttg
    10501 gcaatttctc accgccatgt ttctgtacag cgggccgtgg cacttcgccg ccaacatttt
    10561 cttgctctac ctgctcgggc gcgatttgga atcgattgtt ggatcacgcc acttcttcta
    10621 cctttatttt gctggcctta gcgccggaga gctcggacac ctctttttaa tgccgccgaa
    10681 ttcaatgttg ctggcgagtt ccggcggcgt aaccgcactc atcgtcgcgt atgcgctcgt
    10741 cctgccggag ctggagctac tcccaagcgg gtcactcttt cgttcgctgc ggttgaaagc
    10801 gaaacatctc gcttatgcga cgattgcact cgcgctgata ttcgcatgcg tgtttcgcag
    10861 cggcaccctc acgcacagtg cgtggctcgg cggttgcgcc gccgggtggc tctacgcaca
    10921 tctgctcgga ttcgggcgtc cgtcgtttct tcagcggttt ctgcagcatc ggcgcgagca
    10981 agtccaccgc tacgaccgca tgactgtgga ggagctgatc gcccaggaaa ttgatccgct
    11041 tctcgacaag atcggccaca gtggatttgg caaacttagc cgccgcgaac ggcgcgagct
    11101 gcttcgcgcc cacgccaaat tactcgagaa gcaagaggta ccctaattcc gaacgcggcg
    11161 acacgtcgcg gcctgaagga gcgcggacgt attcgcgcac tgcagacgtc ctagctcgcg
    11221 aactggtctt tgggcggaac cggcggcatg ttgcgcagga tggactcggt tgatcgctgg
    11281 atcgagcaga aggcggttac gacttttcat ccggcgcaag tggaagcgag cctgcgcagc
    11341 ctgcatgatg cctggccggc cgaggcgccg ccgctcgccc gcatgatcga gcaatttcca
    11401 ctcggcgaag cagccctcct ccatctgttc gccgcgtcga gcatctgcgc cgcgcgatcg
    11461 gtgcgcgacc cgacgattct tctctggctg gcagagccaa aaatctgcgg ctcacaacgt
    11521 ggctacgcgg aaatgagcga agcgctgcac gccatgtctc gcaatgggct cgcggctaat
    11581 cattttcgcg cgcttcggca atggaaagcg cgcgagatgg tgcgaatcgc gttgcgcgaa
    11641 gtcgccaatg cggcggcgct cgaggaaaca acggccgagc tgtcgcagct ggcagagatt
    11701 tgcatccgct gcgtctacga acattgcaac gccaaattgc ggaggcggca cggctcgcca
    11761 caagcggagt tcgcaatcct ggcgctcgga aaattaggtg gtcgcgagct caatcacagc
    11821 tcggacgtcg atcttctatt tgtttatagc gcagatggcg aactggtgcc cggccgttcc
    11881 taccacgagt tcttcaatga acttgccgaa cagatccttg agacattttc gcgaccgcac
    11941 ccggaaggtc cgcttctgcg cgtagacgtt cgtctgcgcc cggagggtcc ggccgggccg
    12001 ctggcccgct cggtcgatag catggagcat tactacggag gcttcggcga aacgtgggaa
    12061 cgtctcgcgc tgattaaagc gcgacacgtc gccggcagtc gcgaagtcgg ctacgatttc
    12121 ctgcgccagc ttcaaacatt catttatccg aagagcgcga cgcctgatct gctcgaagag
    12181 atcgccggca tcaaacaccg catcgagcgc gatgtcgttg gcgaacgcaa actgggccgc
    12241 aacgtgaagc tcgggcgcgg cggcattcgc gagatagagt tcatcgtcca gactctgcag
    12301 ttcgtccacg gcgcccggca cacgttccta caagagccaa acacgttgaa agcactgcgc
    12361 gctttggcgc aactcgaact gctcccgcgc agtgaaattt tcgatcttga tcgggcctat
    12421 cgcttcctgc ggcgagtcga acaccgcttg caaatcgaag ccgaagaaca gacccacact
    12481 attccggcag atccggagcg cttgcgccgc ctggcgcgaa gtctggagtt cgagtcggcc
    12541 gacaaatttc aaactgcgct ggaacgcgag atgacagcgg tgcggtgtct tttccggaaa
    12601 gtgattgcgg aagcgccggg cagccaggag accggcgcac tcaatctcga cattttttcc
    12661 gagcgcgcag ctgcggagcg cgcgatggct gacctcatgc acggtgccgg cgccgcgcac
    12721 acctcaaaac ggcggcgaca atctgcccgg cggctccagc ccgtcctgct tggtcaattg
    12781 gcaaagactg ccgaccgcga cgcagccttg acaaaattcg tccggttcgt cgaagcccac
    12841 gggatgcgca gcttgctttt tg
//
LOCUS       DASTDG010000206         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_541747, whole genome shotgun sequence.
ACCESSION   DASTDG010000206 DASTDG010000000
VERSION     DASTDG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541747"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1286)
                     /locus_tag="VFO30_06715"
     CDS             complement(54..1286)
                     /locus_tag="VFO30_06715"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9419016.1"
                     /translation="MHIPSLIEKKRDGHALTVEEIKTLISDFTFGDVPDYQMSAWAMA
                     VFFRGMTPAEARHLTEAMMQSGRVFKYPKDSPPKIDKQSTGGVGDKVSLVLAPLLACD
                     QVWVPMICGRGLGITGGTLDKLESIPGFNVNLDDKRALKQLDKIGVFMIGQTEDICPA
                     DKKLYALRDVTGTVPSQPLIVASIMSKKLAENLDRLVLDVKFGRGAFMKTKKDAEQLA
                     AAMTEVGASMGVKTTHLLSPMDEPLGRAVGNALEVAECVEILQGGGPPDVVKLIVDLA
                     EKVSETPRAKLKQRLKDGSAWKKFVALVYAQDGDATALEKMTEVHRAPVLEPLRAKSA
                     GRVKKMDAGLIGRASVLLGAGRQRTGDMIDFAVGFSDIKKLGEYVDLDEPLLTVHARN
                     EQSLHSAMPLLEKAIAVG"
     gene            1347..1868
                     /locus_tag="VFO30_06720"
     CDS             1347..1868
                     /locus_tag="VFO30_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET9419017.1"
                     /translation="MIRIDWRSFVVTDGEATQNGSAPNSSKARASPLTARSNARPFFS
                     PKSATGARALQRDVRIAQAVAKGDRKASVPTQRLTVRKGCDYSDHMTTTLREAKAKLS
                     QLVKAASRGEEVVITVHGKETAKLVGMPNNQRRIDRERWLKRLERLRARNWTGRYGKT
                     AQEIQDEDRAERF"
     gene            1816..2310
                     /locus_tag="VFO30_06725"
     CDS             1816..2310
                     /locus_tag="VFO30_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET9419018.1"
                     /translation="MGKRLRKSRTRIARSDFSVAFWDTSALLKLYVRERDSDRFAELI
                     RTSIDRTVISQLTLAEMHRALWGKALARAIPAKLADTTYREFRSDIEAVALTVLPLGR
                     EVEHEFDRIVPLCYRANPAVPIRTLDGLVLASAVVARTPELVSTDSRMRRAAVLLGLR
                     ILPE"
     gene            2311..>2552
                     /locus_tag="VFO30_06730"
     CDS             2311..>2552
                     /locus_tag="VFO30_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9419019.1"
                     /translation="MSMHKSDKIFVAGHRGLVGSAILRRLRADGYGNLLTRERSQLDL
                     TDTKAVNDLFAKEKPSVVVLAAAKVGGIKANNDFPV"
BASE COUNT          521 a          710 c          772 g          549 t
ORIGIN      
        1 tcaatgaagt attgcgaacg actgcttttc gctcgttgag atatttttgt cgctcagcca
       61 accgcaattg ctttttcgag cagcggcata gcggaatgaa gcgattgctc gttgcgcgcg
      121 tggactgtta gcaatggctc gtcaagatcg acatattcgc cgagtttttt gatgtcggaa
      181 aacccgacag cgaaatcgat catgtcgccg gtcctttgcc gcccggcgcc gagcagaact
      241 gaggcgcggc cgattaaccc ggcgtccatt ttcttaactc ttcccgccga tttcgctcgt
      301 aacggctcga ggacgggcgc gcggtggact tcggtcattt tctcaagcgc ggtggcgtcg
      361 ccgtcctgcg cgtaaacgag ggcgacaaat ttcttccagg cgctgccgtc ttttaggcgt
      421 tgcttcagct ttgcgcgcgg cgtttcggaa actttctccg caagatcgac aatcaacttc
      481 accacatcgg gtggcccgcc gccctgcaaa atttcgacgc actcggcgac ttcgagcgcg
      541 ttgccgacgg cgcggccgag cggctcgtcc atcgggctga ggagatgggt ggtcttcacg
      601 cccatggatg caccgacctc ggtcatcgcc gcggcgagtt gctcggcgtc cttttttgtt
      661 ttcatgaacg cgccgcgacc aaatttcaca tcgagcacga gccgatcgag gttttcggcg
      721 agctttttgc tcatgatact agcgacgatg agcggttgtg aaggcacggt gccggtcacg
      781 tcgcgcagcg cgtaaagttt tttgtcggcc gggcaaatgt cctcggtctg gccgatcatg
      841 aacacgccga ttttgtcgag ttgtttcagc gcgcgcttgt cgtcgaggtt gacgttgaag
      901 ccggggatgc tctcgagttt gtcgagcgtg ccgccggtaa tgccaagacc gcgcccacag
      961 atcatcggca cccagacttg atcgcaggcg agcaacggcg cgagcacgag tgagacctta
     1021 tcgccgacgc cgccggtgga ctgtttgtcg atcttcggcg gcgagtcttt ggggtactta
     1081 aacacgcgcc cgctctgcat catcgcctcg gtgagatggc gcgcttccgc cggggtcatt
     1141 ccacgaaaga acacggccat cgcccaggcg ctcatttggt aatcgggaac gtcgccgaag
     1201 gtgaagtcgg agatcagcgt tttgatttcc tccacggtaa gcgcgtgacc gtcgcgcttt
     1261 ttctcgatga gcgatgggat gtgcatttta accgccaatg gagaccacta aagaagcgcc
     1321 cgggcacgac acccgaaccg tgaaatgtga ttcgtatcga ttggcgttca ttcgtggtta
     1381 ccgatggaga agccactcag aacggctcag cgccaaatag tagcaaggcg cgcgcctcgc
     1441 cgctcacagc ccgatcgaat gccaggccat ttttcagccc caagtctgca actggcgcga
     1501 gagccttgca gcgcgacgtc aggattgcgc aagccgttgc aaaaggcgac aggaaagcat
     1561 ctgtcccgac acaacgtttg accgttcgca aggggtgtga ctacagtgac cacatgacca
     1621 cgaccttgcg ggaagccaaa gccaagcttt cacagctggt caaggcggcc agtcgcggcg
     1681 aggaagttgt aatcacggtg catggaaaag aaacggcaaa gctcgtcggc atgccgaaca
     1741 atcaaaggcg aattgatcgg gagagatggc tgaagcggct tgagcggctg cgggcgagaa
     1801 attggacggg ccggtatggg aaaacggctc aggaaatcca ggacgaggat cgcgcggagc
     1861 gattttagtg tggcgttttg ggacacgtcg gcgctgctca agctttacgt tcgggaacgc
     1921 gattccgatc ggtttgcgga attaatcaga acctcgattg atcgaactgt gatttcacaa
     1981 ctgactctgg ccgaaatgca ccgcgcactc tggggaaagg ctttggcccg cgccattcca
     2041 gcgaagctcg ccgatacgac gtatcgtgaa tttcgtagcg acatcgaagc cgtggcgctc
     2101 acggttctgc ctcttggacg agaagtggag catgagttcg accgaatcgt tccgctttgt
     2161 tatcgagcaa atccagcggt gccgattcgc actctcgatg ggctggttct cgcctcagcg
     2221 gtagtggctc gaacgccgga gctggtaagc accgattctc ggatgcgccg tgctgcagtt
     2281 cttctcgggc tgcgaatctt gccggagtaa atgtcgatgc ataagtccga caagatcttc
     2341 gtggcgggcc accgcgggct ggtcggcagc gcgattctgc ggcgcctgcg ggccgacggg
     2401 tacggcaatt tactcacccg cgagcgttcc cagctggatc ttaccgacac gaaagcggtg
     2461 aacgacttgt ttgcaaaaga aaaaccgagc gtggtcgttc tcgccgccgc aaaagtcggc
     2521 ggcattaagg cgaacaacga ttttccggtc ga
//
LOCUS       DASTDG010000207        13093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1514447, whole genome shotgun sequence.
ACCESSION   DASTDG010000207 DASTDG010000000
VERSION     DASTDG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514447"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFO30_06735"
     CDS             <1..529
                     /locus_tag="VFO30_06735"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9419020.1"
                     /translation="GLFFGRCANFINGELYGRAATVGWAMQFPKELLDHPAEAERAIA
                     ACTAIHPALNSPEAIIAAVRGQPQVKEVLRAILTPRHPSQLYEAFLEGIVLFALLWFV
                     RTRMRVANGVLTGLFFIVYAVFRIAIENFREPDASLIAGVTRGQFFSFFLIVVGAAFV
                     IAATRHPTFPKQMSI"
     gene            586..1341
                     /locus_tag="VFO30_06740"
     CDS             586..1341
                     /locus_tag="VFO30_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9419021.1"
                     /translation="MFPSVCLAAGGLLFAFEHATIAGKLVLMLLAIGSIFSWSVMITK
                     LRVIQFARKQNARFLAAFRQDRQPLRLFEKHARFPGSPNFNVYRAGCQEMTFHLLGSP
                     EVDETFRARLDIADKISPPQMGAVTAAMERAVGETALELESQMILLATAVSGSPFLGL
                     LGTVWGVMDAFTGVAEAGAPNLAAMAPGVSGALITTVTALCVAIPAMFGYNFLVTRIR
                     GMIVEMDNFAAELASDFEHKFVDHGAREPAFRR"
     gene            1338..1754
                     /locus_tag="VFO30_06745"
     CDS             1338..1754
                     /locus_tag="VFO30_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9419022.1"
                     /translation="MRRYSQRNNLSTLSEINITPLLDLAFVLLIIFMITTPLLENSMS
                     LVIPSSSERTRPINSSQVQTVSIDRAEVIKLNNEVVDLARLSSRLAELKQSSADVAVV
                     IRSDRELPVQHLVALIDVLQRAGITKVGIATRAEAK"
     gene            1757..2680
                     /locus_tag="VFO30_06750"
     CDS             1757..2680
                     /locus_tag="VFO30_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9419023.1"
                     /translation="MPKNARFWRNVTLIGFAHTAVIAGFVHWSRESKKAPAQSIVWMG
                     GSGSEGSVTTETPPPSTISARPPVREPDLDSESRTESASERDRPVLASAKSEIELPAA
                     RSSATSAPTRKPSATPKVKVTPHPTPKPKPRPTATPKPKPSPRKVLLAKASPKPLPSR
                     TENKEEETNAEEQLKAPTAPALSKTGSRTGEEKEAPVGVGAGRGAGGSNQSQFGWYGS
                     MLHDRFYSEWVQPTNVGTSGAKNSVLAKLRIENDGRVSNFEIVKPSGNAMVDESVAAV
                     GKRVTQVDPLPPGLGDGEHYDVKINFELNSE"
     gene            complement(2731..3741)
                     /locus_tag="VFO30_06755"
     CDS             complement(2731..3741)
                     /locus_tag="VFO30_06755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9419024.1"
                     /translation="MPFAIKPPLAPMETKSVGEIPAGDNWQYEPKWDGFRCIAFRDNE
                     NVYLQSKAGQPLGRYFPDVVEAIGNLPGKNFVLDGELVIPVNGALSFDELQLRLHPAA
                     SRVAQLAKAHPALFIAFDLLAQDRQSLLARPLHERRALLEKFKMKGAVKLSPATTKLE
                     RAKSWFDEVGGDLDGVIAKLLDAPYARGERTAAMKVKQIRSADCVVGGFRYASGAKIL
                     GSLLLGLYGDDGLLHHVGFTSAFKADERRALTKKFEALCKPPGFTGNAPGGPSRWSTE
                     RSGEWEPVEPKIVVEVAYDHFTGGRFRHGTRILRWRPDKASRQCTMDQVAGREGKSLA
                     LL"
     gene            complement(3824..4480)
                     /locus_tag="VFO30_06760"
     CDS             complement(3824..4480)
                     /locus_tag="VFO30_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419025.1"
                     /translation="MAAKLDNRKRNRIFLLSPAYAGGERARIIMSERAEFDLARRLRS
                     EGAPLAEVFTFLSGLYFRGKIAYANAFARVTDGLCGALVITPTRGLVDARSTITLDHL
                     REFAEVDVHLADARYRRPLERDTRKLAKKLSPQCEVILLGSIASNKYVEVLLTHFGER
                     LRFPVDFVGRGDMSRGGLLLRGAADRQELSYMSVKGAVLHGVRPPKLEPRRYPRNSPI
                     "
     gene            complement(4526..6109)
                     /locus_tag="VFO30_06765"
     CDS             complement(4526..6109)
                     /locus_tag="VFO30_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET9419026.1"
                     /translation="MQPAETIVILFAFVAALVLVSRKLTLPYPIVLVVAGLALSFIPR
                     LPQVKLNPEIVFYFFLPPLIYPAALFTSWRDFSRNLRPIGLLSIGLVLFTIGVVAWAA
                     HALVPTMSWPAAFAFGAIVSPTDAIAATSVIRRLSLPHRIEVVLEGESLVNDATALVA
                     LQFAVAALMTGSFSLSAAAGRFVVVVLGGIALGLLVGIVMRWIHRHLDDPPVQITISL
                     LTPFIAYLPAERLHLSGILAVVAAGIFLGWHTPLIVTARYRLQAFAFWEMVVFLLNGF
                     VFIVIGLQLPGILHALRGESLVRLITDGLLVSAGAVLIRIAWVFPATYLPRFLFPRIR
                     ERDPYPDWRNVLVVAWCGMRGVISLAAAFALPFSLSNGAAFPARNYILFLTFCVIFTT
                     LVLQGLTLPLLIRLFRIEPDTAIADEERAARVEANKAALQFIEHAATADGFAPNVIDR
                     LRIEYEERLDQLRISADTTAERRGEVATREYVQLQNQALKREREAIIHLRNQHVINDA
                     ALRRIQRDLDLAEARLAAG"
     gene            6190..7218
                     /locus_tag="VFO30_06770"
     CDS             6190..7218
                     /locus_tag="VFO30_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9419027.1"
                     /translation="MKDFLPAAELLLGEIQSRHASAAEATRSDAALLDEYSRTIIGAV
                     QRVAPTVVNIEVRQRNERSHAREIAGSGSGFIITPDGFILTNSHVVHDATNTTVNLND
                     GRDFPAQLMGDDPDTDLAVIRIGAPNLAHVRLADSEQVQVGQLAIAIGNPLGFQASVT
                     AGVISAVGRSMYGQSGRLIDNIIQTDAALNPGNSGGPLVNSAGEVIGVNTAMIRPAQG
                     ICFAIASNTAKLIAGWLIRDGKIRRSYIGVAGQTAPLHRRVIRFYHLPLETGVLVVSI
                     EKNSPAERAGLRAGDLIVAFNHQPIGSVHDLHKILVGEQIGVSTTVTIIRHTEKLDLS
                     ILPAEMAH"
     gene            7218..8489
                     /gene="purD"
                     /locus_tag="VFO30_06775"
     CDS             7218..8489
                     /gene="purD"
                     /locus_tag="VFO30_06775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET9419028.1"
                     /translation="MKVLVIGSGGREHALAWKLKQSPAVDRIFCAPGNGGTEEVAENV
                     GITATDFEALVRFAKQNGVDLTVVGPDDPLALGIVDVFTSEKLRIFGPSKSAARLESS
                     KIFAKELMRSRKIPTAEAKTFTNSDEALRYCERLQFPVVIKADGLALGKGVVIAADQA
                     TARSTVAAMMNEDRFGDAGRRIVIEEFLRGSECSLHALVAGEDYLLLESARDHKRVFD
                     GDEGPNTGGMGAFSPANNWSLELKWQFERKIIQPLLHALPEQGITYRGLLYPGVIITA
                     DGPRVLEFNCRFGDPETQALLPRMKSDLLPLLDATIDGGIGSHAIEWDQRAAVTVVLA
                     SGGYPGKYETGHTISGLNDAAKIDNVQVFHAGTKRGKEGIVTAGGRVVAVTALGETIA
                     EARTRAYEAVARIRFEGCHYRRDIALTAVNG"
     gene            8619..9608
                     /locus_tag="VFO30_06780"
     CDS             8619..9608
                     /locus_tag="VFO30_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9419029.1"
                     /translation="MPTATPAIDQLVNSLGGTDVQAAITLLKANFVKSDALTDEELNR
                     ALLQGLLVRYARGLQVLSNPATAPAEPAAPFYSEIFEGHIGYLRFGALNSANLTSLDK
                     KLEEFAAKKTDAMVVDLRDNDSASDFAAAAEFAKRFCPKGKLLFTLRKPAARQDRAFS
                     SDREPAVRALIVVLIDTDTSGAAEAVATALHTYNKALLIGQPTAGRAVEYADLPLPSG
                     KILRVATAETVGPDGQLLFPDGVKPDLPVEMARSDKRQIFALSAEKGMAPFVYETERP
                     HLNEAALITGTNPELESGETQRRNRGREKLPVRDPVLQRALDVIASLEIFQKR"
     gene            9611..10312
                     /gene="rnc"
                     /locus_tag="VFO30_06785"
     CDS             9611..10312
                     /gene="rnc"
                     /locus_tag="VFO30_06785"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9419030.1"
                     /translation="MHPLEERIGYKFRNSLLLAEALTHPSLGHETQERHFDNQRLEFL
                     GDAVLQLVITEHLFRHFGGEPEGKLTKLRSRLVSRDTLRAHAVALDLGRDLLMGRGEE
                     ASGGRERTSTLADAFEALIGAIYLDSNFDEARKFILAQARPELESIAQEPVDINPKGD
                     LQELLQAISPTSPVYETISESGPEHDKTFVVRAMWEGNELGQGSGRSKKQAETAAAEE
                     ALRLRRWEKGERDRT"
     gene            complement(10371..11192)
                     /gene="thiD"
                     /locus_tag="VFO30_06790"
     CDS             complement(10371..11192)
                     /gene="thiD"
                     /locus_tag="VFO30_06790"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9419031.1"
                     /translation="MAAQKNAQQSRPVALTIAGSDSSAGAGIQADLKTFSALGVYGVT
                     AVTCIVAEIPGKVSRIEAVRPGIVREQIRVVASSFPLAAIKTGLLCSADVVNAVAQAI
                     VDFAADVPLIVDPVMVATSGDALLHRNAIKIYEEALFPRATLITPNLDEAAKLLGEKI
                     QDRQSMRRAACALAAKYATAVLVKGGHLRGRQAVDILCAAGKLSEFAAESVRDVATHG
                     TGCTYSAAIAAGLARGQTLEVSIRRAKKFTAATIAQHFRWQAHSGKNLDALNHFL"
     gene            complement(11270..11503)
                     /locus_tag="VFO30_06795"
     CDS             complement(11270..11503)
                     /locus_tag="VFO30_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9419032.1"
                     /translation="MADVANKYAENVFGKFYVDDQCIDCDLCRETAPANFKRNDDGGH
                     SYVYKQPETPEEDVLCKEAMEGCPVEAIGDNGA"
     gene            11626..11868
                     /locus_tag="VFO30_06800"
     CDS             11626..11868
                     /locus_tag="VFO30_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419033.1"
                     /translation="MRTSAAKTLRAFLIELAIYSLLVVVYFFLVLHFLGGWLYGLEAR
                     HRYTYAGVAILLMIGQAVVLQNVTTILLRLIRGRAE"
     gene            11872..12789
                     /locus_tag="VFO30_06805"
     CDS             11872..12789
                     /locus_tag="VFO30_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9419034.1"
                     /translation="MFFPISGAHISPIYLVIVGFVIGILGGFFGVGGSFIAGPALRVV
                     GLDWNFAVGTDLAHIVGKSVVAVRQHRAFGNVDLRLGLVMAVGTIAGAEIGAQLIQML
                     KRAGNVNLVVSIVSIAVYVSISTFMIWESRKSLATKKPLPPASAGGAKKDHSAFRHVT
                     RRIQRLKLWPMIALPVSGVRAISLWVILLVAFVGGLFSGFLGGGAGYIRMPSMVYVLG
                     IPTHLAVGTDLFEIIISASYGTFTHAIKGNVDILIALVMHTGAAIGAQIGAISTQYFA
                     GPKIRLAFVPLPLIGAAIVIYTMISGHKL"
     gene            12796..>13093
                     /locus_tag="VFO30_06810"
     CDS             12796..>13093
                     /locus_tag="VFO30_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9419035.1"
                     /translation="MNILICSDGRAASDNATRLAGLLAPALHAKTTLLGIIEASADEQ
                     ALRRALEKQAESLRASKIAVETVVHPGEPIHEIVHQTSTAKYDLVIIGSRRARST"
BASE COUNT         2776 a         3770 c         3960 g         2587 t
ORIGIN      
        1 cggtttgttt ttcggtcgct gcgccaattt cattaacggt gaactttatg ggcgcgcggc
       61 gactgtcggt tgggcgatgc aattcccaaa agaattgctc gatcatccgg ctgaagctga
      121 gcgcgcaatc gcggcatgca cggccatcca ccccgccctg aactcgccgg aggcgatcat
      181 tgcggcggtc cgcggccaac cacaggtcaa agaagttctg cgcgcgattc tgacgccccg
      241 gcacccatcg cagctctacg aagcatttct cgaaggcatc gttttgttcg ccctcctctg
      301 gtttgtacgg acgcgaatgc gcgtggcgaa cggagtgttg actggtctgt tcttcatcgt
      361 gtacgcggtg ttccgcatcg cgatcgaaaa ctttcgcgaa ccagacgcga gtttgatcgc
      421 gggcgtcacg cggggccaat tcttttcctt cttcctgatc gttgtcggcg cggctttcgt
      481 catcgccgcg acacgacacc cgacgtttcc aaagcagatg tcgatctaac aaaggcgctt
      541 tcgtttccgc caaagcgcat tattacttag ctcggtgatc ggccagtgtt cccctcagtt
      601 tgtctagcgg cgggcggtct gctcttcgcg ttcgagcacg cgacgattgc cggcaagctt
      661 gtccttatgc tccttgccat cggctcgatc ttcagctggt cggtcatgat taccaaactg
      721 cgcgtcatcc agttcgcgcg gaaacagaac gcgcgctttc tcgccgcgtt ccgacaagac
      781 cgacaaccgc tgcgcttgtt cgaaaaacac gcgcgtttcc ccggctcgcc caatttcaac
      841 gtctaccggg ccgggtgtca ggaaatgaca tttcatttgc tcggctcacc cgaggtggac
      901 gaaacttttc gcgcgcgcct cgacatcgcg gacaagattt cgccgccgca aatgggcgcg
      961 gtcacggccg cgatggaacg cgcggtcggt gagacagcgc tcgaactcga gtcgcaaatg
     1021 atcttgctgg cgacggcggt cagcggttcg ccgtttctcg gcctgctcgg cacggtctgg
     1081 ggcgtgatgg acgcgttcac gggcgtggcc gaagccggtg cgccgaacct ggcggcgatg
     1141 gcgccgggcg tttctggcgc gctgattacg accgtaaccg cgctctgcgt cgctatcccg
     1201 gcgatgtttg gttacaactt tctcgtcacg cgtattcgcg gcatgatcgt ggagatggat
     1261 aacttcgccg cggagctggc gtccgatttc gagcacaagt ttgttgatca cggtgcgcgc
     1321 gaaccggcgt tccgccgatg agacgctatt cgcaacgaaa taacctgagc acgctgagcg
     1381 agatcaacat cacgcctttg cttgatctcg cgttcgtatt gttaatcatt ttcatgatca
     1441 caacgccatt gctcgaaaac agcatgagcc tggtgatccc atcgagcagc gagcgaacgc
     1501 gcccgatcaa ttcgtctcag gtacaaaccg tctcgatcga ccgggctgaa gtcatcaaac
     1561 tcaataatga agttgtcgat cttgcgaggc tgagctcgcg tctggcggaa ctgaagcagt
     1621 cgagcgcgga cgtggccgtc gtaatccgct ccgaccgcga gctgcccgtg cagcaccttg
     1681 tcgctttaat cgacgtgttg cagcgcgctg gcattaccaa agtgggcatc gcgacacgag
     1741 ccgaggcgaa atagacatgc ccaagaacgc gcgcttctgg cgcaatgtca cgctgatcgg
     1801 ctttgcgcat acagccgtca ttgccggctt cgttcattgg agccgtgaga gcaaaaaggc
     1861 gccggcccaa agcatcgtct ggatgggcgg ctccggcagc gagggatcgg tcacgaccga
     1921 aacgccccct ccatctacga tttcagccag accgccggtg cgggaaccag atctcgacag
     1981 cgaatcgcgg acagaatcgg cgtcagaacg agatcggccg gtcctcgcat cggcaaaaag
     2041 tgagattgaa ttgccagctg cgcgatcctc cgcaacctcg gcaccgacgc gcaagccaag
     2101 cgcgactccg aaagtgaagg tcacgcccca tccaacaccg aagccaaagc ccagaccgac
     2161 tgcgacaccg aaaccgaagc cgtcgccgcg aaaagtgctt ctcgccaaag cctcgccgaa
     2221 accgttgcca tcacgaactg agaacaaaga agaagaaaca aacgcagaag aacaactgaa
     2281 agcgccaacc gcgcccgcgc tttcgaagac cggctcgcga accggcgagg aaaaggaagc
     2341 gccggttggc gtgggtgctg gtcgcggtgc tggcgggagc aaccagtcgc agtttggttg
     2401 gtacggcagc atgttgcacg atcgttttta cagcgaatgg gtgcagccga ccaatgttgg
     2461 gacaagcggc gcgaagaatt ctgtgctcgc gaaattgcgg atcgaaaacg acggccgcgt
     2521 ttcaaatttt gaaatcgtga agccatcggg caacgcgatg gtggacgaat cggtcgccgc
     2581 ggttggcaaa cgcgtcacgc aagttgatcc gctcccgccg ggactcggcg acggcgagca
     2641 ctatgacgta aaaatcaatt tcgagctgaa ctcggaatga ctgtagccgt cgccctgtgg
     2701 gcgacgcaga cgcttcgcgc aacgaagcga ctacagaagc gctagcgatt tgccttcgcg
     2761 gccagcgacc tggtccatcg tgcactggcg ggaggccttg tcaggacgcc agcgcaagat
     2821 tctcgtgccg tgccgaaaac ggccaccagt gaaatggtca tatgccactt cgaccactat
     2881 cttcggttcg accggctccc attcgccgct ccgttccgtg ctccaacggc tcggaccacc
     2941 gggagcgtta ccggtgaagc cgggcggttt gcacagagct tcgaattttt tcgtgagcgc
     3001 gcggcgctcg tcggctttaa acgccgatgt aaatccgacg tgatgcagca gcccatcgtc
     3061 gccgtacagg cctaacaaga gcgagccgag aattttcgcg ccactcgcgt aacgaaaacc
     3121 accgaccacg caatccgcgc tgcgaatttg cttcaccttc atggcggccg tgcgctcgcc
     3181 gcgcgcgtag ggcgcgtcga ggagtttcgc tattacaccg tcgagatcgc cgccaacttc
     3241 gtcgaaccag ctctttgccc gctcgagttt ggtggtagcc ggcgaaagtt tcaccgcgcc
     3301 tttcattttg aacttctcga gaagcgcgcg tcgttcatgc aatgggcgcg ccaaaagaga
     3361 ttgtcgatct tgcgcgagca gatcgaatgc aatgaacagt gcggggtgcg ctttggccag
     3421 ttgcgcgacg cgactcgcgg cgggatgcag gcgcaattgt aactcgtcga atgaaagcgc
     3481 gccgttcact ggaatgacaa gctcgccatc caagacaaag tttttgccag gcaaatttcc
     3541 gattgcctcg accacatcag gaaaatagcg ccccagcggc tgacccgcct ttgactgcag
     3601 gtagacgttt tcgttgtcac gaaaagcgat gcagcgaaac ccgtcccact tcggctcgta
     3661 ttgccagttg tcgcctgcgg gaatttcgcc aaccgatttc gtctccatcg gcgcgagcgg
     3721 cggcttgatc gcgaatggca tagcaggaat tgtagaggcg tttgtgccaa acgcctggct
     3781 tctaagttcg gcgcttgtca caagcgccgc tacaacagct ttgctaaatc ggcgagttcc
     3841 tcgggtagcg tcgcggctcc agctttggcg gacgcacacc gtgcagcacg gcgcctttga
     3901 ctgacatgta gctcagttcc tgtcgatcgg cggcgccacg cagcagcagt cctccacgac
     3961 tcatgtcacc gcggccaacg aaatcgactg ggaaccgcag acgttcaccg aaatgcgtga
     4021 gcaacacctc gacatattta ttgctggcga tgctgccgag caaaattacc tcgcattgcg
     4081 gcgagagttt tttcgccagc ttgcgcgtgt cgcgttcgag cggacggcga tagcgcgcgt
     4141 cggcaagatg gacatctact tctgcgaact cgcgcaggtg atcgagtgtg attgtcgatc
     4201 ttgcatcgac caagccacgc gttggcgtga tcaccagagc gccgcaaagg ccatcggtga
     4261 cgcgggcgaa cgcgttggca tatgcgattt ttccgcggaa atacagtccg ctcaggaacg
     4321 taaatacctc cgcgagcggc gcgccttcgc tgcgcaagcg acgcgcgaga tcgaactcgg
     4381 cgcgctcgct catgattatg cgggcgcgtt caccgccggc ataggccggc gaaaggagga
     4441 aaattcggtt tcgctttcgg ttgtcgagct tcgcggccac tgaaattgca acttcgtgtc
     4501 gcagccacgc ggcgcaacgt ggaaatcagc cggcggcgag gcgcgcttcg gcgaggtcga
     4561 ggtcgcgctg gatgcggcgg agcgcggcgt cattgatgac gtgctggttg cgcaggtgaa
     4621 tgatcgcttc gcgctcgcgc ttaagcgcct gattttgcag ctggacgtac tcgcgtgtgg
     4681 caacttcgcc gcgacgctcc gcagttgtgt cagcagaaat tcgcagttga tcgaggcgct
     4741 cctcatactc gatgcgtaaa cgatcaatca cgtttggcgc aaaaccgtcg gcggtcgccg
     4801 cgtgctcgat gaattgcagt gctgccttgt tcgcctcgac gcgggcggca cgttcttcgt
     4861 cggcaatcgc cgtatccggt tcgatgcgga acaacctgat caacagcggc agggtcaacc
     4921 cttgcagcac gagcgtcgta aagatgacgc aaaaagtcag aaacaaaatg tagttgcggg
     4981 cggggaacgc cgccccgttc gataacgaaa atggcaacgc gaaggccgcg gcgagcgaga
     5041 tcacgccgcg catcccgcac cacgccacga ccaaaacgtt gcgccagtcc gggtatggat
     5101 cgcgttcgcg aatccgcggg aagagaaacc gcggcaaata tgtcgctgga aaaacccagg
     5161 cgatgcggat gagcacggca cccgcgctga cgagcaggcc atcagtaatt agcctaacga
     5221 gtgactcgcc gcgcaaggcg tgcaggatac cgggcagttg cagcccgatg acgatgaaga
     5281 cgaacccgtt gagcaggaac acgaccattt cccaaaaggc aaacgcctga aggcgataac
     5341 gcgcagtcac gatgagtggc gtatgccagc cgagaaaaat tccagccgcg acgacggcga
     5401 gaatgccgga gagatgcaga cgttcggccg ggagataggc gatgaacggc gtgagcagcg
     5461 agatggtgat ttggaccggc ggatcgtcga ggtgccggtg gatccagcgc atcacgatgc
     5521 cgacgagcaa acccagagca attccgccga gcacgacgac cacgaagcgg ccagccgctg
     5581 cgcttaagga aaaactcccg gtcatcaaag ccgcgaccgc gaattgcagc gccaccagcg
     5641 cggtcgcatc attgaccagg ctttcacctt cgagcacgac ttcgatccga tgcggcaggg
     5701 acaagcggcg gatcaccgag gttgcggcga tcgcgtcggt cggtgatacg attgcgccga
     5761 atgcaaacgc cgccggccaa gacattgtcg gaaccagcgc atgcgccgcc catgcgacga
     5821 cgccaattgt gaaaaggacg aggccaatcg aaagcaatcc gatcggtcga aggttgcgcg
     5881 agaagtcgcg ccaggaagta aaaagcgcgg ccggataaat gagcggcggc aaaaagaaat
     5941 aaaagacgat ctcgggattc aatttcacct gcggtagccg cggaatgaaa ctgagtgcca
     6001 gtccagcgac aaccagcacg atcgggtagg gcaacgtcag tttgcgcgaa acaagaacga
     6061 gcgcggcgac gaacgcgaaa agaatgacaa tggtttcggc tggttgcatc gcaagatcga
     6121 tccgccttcg ctcaaggcta cggcgagacg gggaattgca agatcgacaa tcagcgggag
     6181 agtttttaca tgaaagattt tcttcctgct gctgagcttc tgctcggcga aatccaatcg
     6241 cgacacgctt ccgccgccga agcgacgcgc tcagacgcgg ctctgctgga tgagtactcg
     6301 cgcacgatca tcggcgcagt gcagcgcgtc gcgccgacag ttgtgaatat cgaagttcgg
     6361 caacgcaacg agcgcagtca cgcacgtgag atcgctggaa gcggttccgg tttcattatc
     6421 actcccgatg ggttcatcct gacgaatagc catgtcgtgc acgacgcgac taacacgacg
     6481 gtgaatctta atgacggccg cgattttccc gcgcaattga tgggcgacga tccggacact
     6541 gatctcgccg tcattcgcat tggtgcgccc aatctcgcgc acgtccgtct cgctgattcc
     6601 gaacaagtgc aggtcgggca actcgcgatc gcaattggga acccgttagg gtttcaggcg
     6661 agcgtaacgg cgggcgtgat cagcgcggtc ggacgctcca tgtacgggca gtctggccgg
     6721 ttgatcgaca acatcattca gaccgacgca gccttgaatc ccggaaactc cggcggcccg
     6781 ctcgtcaatt cagccggcga agtaatcgga gtgaacaccg cgatgattcg cccggcgcag
     6841 gggatttgtt tcgccatcgc gagcaacact gcgaagctca tcgccggctg gttgattcgc
     6901 gacggtaaga ttcggcgaag ctacataggc gtcgctggac agactgcgcc gctgcatcgt
     6961 cgagtgattc ggttttatca tttgccactc gagacgggcg tgctggttgt ttcaatcgag
     7021 aaaaacagtc cggctgagcg cgccggtttg cgcgcaggcg atctcattgt cgcgttcaac
     7081 catcagccga tcggcagcgt ccacgatctg cacaagattt tggtcggcga gcaaatcggg
     7141 gtgagcacga cggtgaccat aatccggcac acggagaaat tggacctgtc gatcttaccc
     7201 gccgaaatgg cgcattgatg aaggttctcg tgatcggcag cggcggccgg gaacatgcgc
     7261 ttgcttggaa gctaaaacaa tctccggccg tcgatcgcat cttttgcgcg ccggggaatg
     7321 gcggaactga ggaagtcgcg gagaacgttg gcattactgc gactgacttc gaggcgctgg
     7381 ttcgcttcgc caaacaaaac ggtgtcgatc ttaccgtggt cggaccggac gatccgctcg
     7441 cgctcggcat cgtcgatgtt ttcacgagtg aaaagctgcg gatcttcgga ccgagcaagt
     7501 cggctgcgcg cctcgagtca tccaagattt tcgcaaaaga gctgatgcgc tcgcgaaaaa
     7561 ttccgaccgc cgaagcgaag actttcacta acagcgatga ggcgcttcgc tattgcgagc
     7621 ggctccagtt tcctgtcgtg attaaggccg acgggctcgc gctcggtaaa ggggtcgtga
     7681 ttgcggctga ccaggcgaca gcaagatcga cagtcgccgc gatgatgaac gaggaccgct
     7741 tcggcgacgc tggccggcgg atcgtgatcg aagaatttct gcgcggcagc gaatgctcgc
     7801 tgcacgcgct ggttgccggc gaagattatc tgctgctcga atcagcgcgc gaccacaagc
     7861 gggtgtttga tggcgatgaa gggccgaaca cggggggtat gggtgcgttc agcccggcga
     7921 acaattggag cctggagctg aaatggcaat tcgagcggaa gatcatacag ccgctcctgc
     7981 acgcgttgcc tgagcaagga atcacctatc gcgggctgtt atatcccgga gtcataatca
     8041 cggctgatgg tccgcgcgta ctcgagttca attgccgctt cggcgacccc gagacgcagg
     8101 cgctgctgcc gcggatgaaa tcggatttat tgccgctact tgacgccacg atcgacggcg
     8161 gcattggcag ccacgcaatc gaatgggacc agcgcgcggc agtgacggtg gtgctggcgt
     8221 cgggcggata tcccgggaag tacgagaccg gccacacgat ttccgggctg aacgacgccg
     8281 ccaagatcga caatgtgcaa gtgttccatg ccggaacaaa acgcggtaaa gaaggaattg
     8341 tcacagccgg cggacgcgtc gtcgcggtta cagcgttggg cgaaaccatt gccgaagcgc
     8401 gcacccgcgc ctatgaagcc gtggcgcgga ttcgcttcga gggttgtcat taccgccgtg
     8461 atattgctct gactgcagtg aacggttaac aatcgagggc aatgtttcac cgggtggcga
     8521 cagcagcggt ctcgatctcg ttgggcataa cagcgttcgc gcaatcgcca tctcccaccg
     8581 caacctcgac aaagccgtcg acggcaacac cgacgccgat gccgacagca actccggcaa
     8641 tcgatcagtt agtcaattcg ttaggcggaa ccgacgtgca ggcggcgatc acgttactca
     8701 aggcgaactt cgtcaagtcc gacgcgttga cggacgaaga actaaatcgc gcgttgctgc
     8761 agggattgct tgtgcgctat gcgcgggggt tgcaggtgtt gtcgaaccca gcgaccgcgc
     8821 cggcagagcc ggctgcaccg ttttacagcg aaatctttga aggacatatc ggttatctgc
     8881 ggtttggcgc tctgaacagc gcgaacctga cctcactgga caagaagctg gaagaattcg
     8941 ccgctaagaa aacagacgcg atggttgtcg atcttcgcga caacgatagc gcgagtgatt
     9001 ttgccgcggc agcggaattc gcgaaacgtt tttgcccgaa agggaaactg ctcttcacct
     9061 tgcgcaaacc ggcggcgcgc caggatcgcg cgttcagttc cgaccgcgag cctgcagtgc
     9121 gtgcgttgat cgtcgtgtta atcgacaccg atacctccgg cgcggccgaa gcagtggcga
     9181 cggcattgca cacgtataac aaggcgttgc tcatcggtca gcccacggcc ggccgcgcgg
     9241 tcgagtacgc cgacctgcca ttgccgagtg gtaaaatcct gcgggtcgcg acggcggaaa
     9301 cagttggacc cgatgggcag ttgctgtttc ccgacggtgt gaaacccgat ctgccggtcg
     9361 aaatggcgcg aagcgataag cggcaaatct ttgccttgag cgcggaaaaa ggaatggcgc
     9421 ccttcgtcta cgagacagag cggccacact tgaacgaagc ggcgctgatc accggcacta
     9481 atccagagct tgaatcgggc gagacgcagc gccggaatcg cgggcgcgaa aaacttcccg
     9541 tgcgcgatcc agtcttgcag cgcgcgctcg acgtgattgc gtcgctcgaa atttttcaaa
     9601 agcgataagc gtgcatccgt tagaggaacg aatcggttac aagtttcgca actcgctgtt
     9661 gctggcggag gcgttgacac atccgagtct tgggcacgag acccaggaac gacacttcga
     9721 taatcagcgg ctcgaattcc tcggcgacgc ggttttgcaa ctggtgatca ccgaacatct
     9781 ctttcgccat tttggcggcg aaccggaagg gaaactgacc aagctgcgtt cgcggctggt
     9841 ttcccgcgac accttgcgcg cgcacgccgt ggcgctcgat ctcgggcgag atttgctgat
     9901 gggtcgcggc gaagaagcaa gcggtggccg cgaacgcacc tcgacgctgg cggatgcttt
     9961 tgaagcactc atcggggcga tttacctgga cagcaacttc gacgaagcgc gcaaatttat
    10021 tctcgcacag gcgcggcccg aactggagag cattgcgcag gagccggtcg acattaatcc
    10081 aaaaggcgac ctccaggaac tactccaggc catctcgccg acgagtccgg tttacgaaac
    10141 gatttctgaa agcggccccg agcacgataa aacattcgtt gtccgcgcca tgtgggaagg
    10201 caacgagctc gggcaaggga gcggccgcag caagaaacag gcggaaacgg cggcggccga
    10261 agaggcgctg aggttgaggc gctgggagaa gggagaaaga gatcgcacgt aggcaatctg
    10321 tagccacgtg cctgtggcac gtccttgcat gatggcgcgc agcgccgtgg ctacagaaaa
    10381 tgattgagag cgtcgaggtt tttacctgaa tgcgcttgcc agcgaaaatg ctgggcgatg
    10441 gttgcggcgg tgaatttttt cgcgcgtcgg atcgacactt ccagcgtttg accgcgcgct
    10501 aagcctgccg cgatggcagc tgagtaggta caaccggtcc cgtgtgtcgc gacatcgcga
    10561 acagattccg ctgcaaactc gctgagctta ccggccgcgc acaaaatatc gacggcttgt
    10621 cggccgcgca gatgtccgcc cttcactagt accgcggttg cgtatttggc cgccagcgcg
    10681 cacgccgcgc gccgcatcga ttgtcgatct tgtatttttt cgccgagcag cttggcagct
    10741 tcatcaagat tgggagtgat cagcgttgcg cggggaaaca acgcctcctc gtaaatcttg
    10801 attgcgttcc ggtggagcag cgcatcgcca ctcgtcgcta ccatgacagg atcgacaatt
    10861 agcgggacat cggctgcgaa atcgacaatt gcttgcgcaa cggcattgac aacgtcggcg
    10921 gagcaaagca gaccggtttt gattgcggcg agcgggaagc tgcttgctac cacccggatt
    10981 tgctcgcgca cgatccccgg acgtacggct tctatccgcg aaactttccc cggaatctcg
    11041 gcgacgatgc atgtgacagc cgtcacgccg taaacaccga gcgcgctgaa tgttttcaag
    11101 tcggcctgga taccggcgcc ggcactgctg tctgacccgg cgatggtaag cgcgacgggc
    11161 cgagattgct gggcgttttt ttgcgcggcc acgccgtaag ctcgccgctg aggacagcgg
    11221 cctctacaga aatttctgct gccagcaacg gcggcagtta caacgaagct taggcgccgt
    11281 tgtcgccgat cgcctcgacc gggcaacctt ccattgcctc tttgcaaagc acatcttcct
    11341 cgggtgtttc cggctgcttg tagacgtagg aatggccgcc gtcatcgttg cgtttaaaat
    11401 tcgccggggc ggtttcccgg cacaaatcgc agtcgatgca ttggtcatcg acataaaact
    11461 tcccgaaaac gttctcggcg tatttatttg ctacgtcagc catgatctgc ccttttttgc
    11521 ggagtggaac ggtagaagaa aacaggggtt gcgcaatgca atttgcgcct cgcgccgatg
    11581 ctgcgcaacc accggatggg ccagaggcga atagtcagcg acgagatgcg aacaagtgcg
    11641 gctaaaacgt tgcgggcctt cctcattgaa ctggcgatct acagtctgct ggtcgtcgtt
    11701 tatttctttc tcgtcctgca ttttctcggc ggctggttgt acgggctgga agcacgtcat
    11761 cgctacactt atgccggcgt cgctatcctg ctcatgatcg ggcaggcggt cgttctccaa
    11821 aacgtgacca cgattttgtt gcggttgatt cgaggccgcg cggaataagc catgtttttc
    11881 cctatctccg gcgcgcatat cagcccgatt tatctggtca tcgtcggatt cgtgatcggc
    11941 attctgggcg gcttttttgg ggtcggcgga agttttatcg ccgggccggc tctgcgggtt
    12001 gtcggtctgg attggaattt cgcggtcggc accgatctgg cgcacatcgt cggtaaatca
    12061 gtggtggcgg tgcgccagca tcgcgccttt ggcaatgtcg atcttcgcct cggattggtc
    12121 atggccgtgg gtacgatcgc cggcgcggaa atcggcgcac agttgattca aatgctgaag
    12181 cgcgcgggaa acgtcaatct ggtcgtgagc atcgtctcga ttgcggttta cgtgagcatc
    12241 tccacattca tgatttggga gagccgcaaa tccctcgcca cgaagaaacc attgcctccg
    12301 gcctcggccg gcggggcaaa gaaggatcat tcggcattca gacacgtcac acgccggatc
    12361 caacggctga aactttggcc gatgatcgcg ctgccggtct cgggcgtgag agcgatttcg
    12421 ctctgggtca tcctgctggt agcgtttgtc ggcgggctct tcagcggttt ccttggcggc
    12481 ggcgccggtt acatccgcat gccatcgatg gtttacgtac tgggaattcc aactcatctc
    12541 gccgtcggca cggacctatt tgagatcatt atctcggcaa gctacggaac gttcacccac
    12601 gcgatcaaag gcaacgtcga catcttgatt gcgctggtca tgcacactgg cgcggccatt
    12661 ggcgcgcaga tcggcgcgat ctcgacgcag tatttcgccg ggccgaaaat ccggctggca
    12721 tttgtcccgt tgccactaat cggcgccgcc attgtcatct acacaatgat tagcggccac
    12781 aaactgtagg gagcgatgaa tattttgatt tgcagcgatg gcagagccgc atcggataac
    12841 gcgacgcgcc tggccgggtt gctcgcccca gccttgcacg cgaaaacgac tttgcttgga
    12901 atcattgaag cgtcggctga cgaacaagca ttgcgcaggg cgctggaaaa acaggctgaa
    12961 tcgctccgag ccagcaagat cgcggtggag acggtggtgc accccggtga gccgattcac
    13021 gagattgtcc atcagacctc aaccgcgaaa tacgatctcg tcatcatcgg ctcacggcgg
    13081 gcgcgctcga ctg
//
LOCUS       DASTDG010000208         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_421440, whole genome shotgun sequence.
ACCESSION   DASTDG010000208 DASTDG010000000
VERSION     DASTDG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421440"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFO30_06815"
     CDS             complement(<1..696)
                     /locus_tag="VFO30_06815"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9419036.1"
                     /translation="MRRITLSLGLFFIIDLSGHAQIDRITGKNFATRSEVVARHGMVC
                     TSVPLATQVGIDILKRGGSAVDAAIAANAALGLMEPVSNGIGGDLFAIVYSAKENKLY
                     EINGSGRSPLGLTYEQIKAELDKLHRTTIPPFGMLPISVPGTVDAWAELHNKFGKLKL
                     ADDLAPAIRYAEEGFSVSELIAYYWAFGPRLYKDLPGAFFETYTLDGKGRTPAKGDVF
                     KNPALAKTLRSIGD"
     gene            760..1188
                     /locus_tag="VFO30_06820"
     CDS             760..1188
                     /locus_tag="VFO30_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419037.1"
                     /translation="MNWKKFFIALVAAFVFIFLFGFIWYGMLMNPIHREVPALFRPEA
                     DFRSYFGWLIFGHLVMAFFLTLLCARYTPAGGAGGGARLGFMTALIYAGADFITYAVQ
                     PLTTKMLAGWIVGDLLMFAIAGAIIGAIYRPGPLTSGANV"
     gene            complement(1197..1529)
                     /locus_tag="VFO30_06825"
     CDS             complement(1197..1529)
                     /locus_tag="VFO30_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9419038.1"
                     /translation="MQLPVNITYRGLEKSDKIDNLVMDYAARLEKFCDHINRCDVAIE
                     QTNRTHQKGNPYRCRIDVTVAPRHELVADEKQMDNGSHEPLAKVIHDAFKTMERQLRH
                     LVEKQRRE"
     gene            complement(1546..2013)
                     /locus_tag="VFO30_06830"
     CDS             complement(1546..2013)
                     /locus_tag="VFO30_06830"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HET9419039.1"
                     /translation="MIPTSFIIVADRGSLKAYRVDETPTRGPSLQLVQAFNLTDAHGK
                     LIDKVSDLAGRFPVTDAAGSHRGAASIAERTQLENETDRRIYKQLADEIAKIVTENGK
                     EGWSFAAPAEMHSAIVDLLPARVRDRIVEHVKSDLVKIEPAKLPAHFRSLRPI"
     gene            complement(2132..4375)
                     /gene="glgX"
                     /locus_tag="VFO30_06835"
     CDS             complement(2132..4375)
                     /gene="glgX"
                     /locus_tag="VFO30_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET9419040.1"
                     /translation="MTPIKAWPGRPYPLGATWLGNGVNFALFSQRATAVELCLFDGLD
                     ATEENIRVPVTERTNYIWHVFLPDARPGQLYGFRVAGSYEPERGLRFNSSKLLLDPYA
                     KAIAGEINWADEMFGYVVGDELEDLSCDFRDNAWGMPKAVVVDLAFDWGDDKRPDTPL
                     AESIIYEVHVKGFTQLCPGVPENLRGTYAGLACDAALAYLKNLGITAVELLPVHAHID
                     DKILADRGLTNYWGYNTIGFFAPHGEYSSSGQMGEQVGEFKAMVRKLHGAGLEVILDV
                     VYNHTAEGNHLGPTLCFRGIDNIASYRLLADNPRFYLDYTGTGNTLDTLDPHTLQLVM
                     DSLRYWASEMRVDGFRFDLAPALARDGAGVNKFHAFFKAIQQDPVLSRVKLIAEPWDV
                     GDGGYQVGNFPTGWSEWNGKFRDAVRSYWKSDEEKIGEVAYRLTGSPDLYQHDDRRPY
                     ASVNVVTTHDGFTLNDLVTYNEKHNEANGENNQDGDHNNQSWNCGVEGPSDDAKINAL
                     RRKQRRNFLMTLFLSQGVPMLLGGDEFGRTQNGNNNAYCQDNEISWFNWQRDDEQLRL
                     LEFTKKLIQLRKRHPVFRRPKFFRGRRIRGSEIKDVMWFNPGGNEMKQEEWSSAFVRC
                     LGMLLSGDATDVVDSQGEPVRDDTFLLLINGHYDVMPFMLPGEENLEWELILDTANED
                     GFIAEPRKCSSGDDADLAGRSACLFRLTRGLQVQARQESWKKREIGLPEAFSAQEERA
                     GGRKAPE"
BASE COUNT          860 a         1317 c         1252 g          994 t
ORIGIN      
        1 gtcgccgatc gatcgaagcg tcttcgccag cgccgggttt ttgaaaacgt cgcctttcgc
       61 cggtgtgcgc cctttgccgt cgagtgtata ggtttcgaag aacgcgccag gtaaatcttt
      121 gtagagccgc ggaccgaatg cccagtagta ggcgatcaat tcgctcaccg aaaaaccttc
      181 ctcggcgtaa cgaatcgccg gcgcgagatc gtccgcgagt ttgagtttgc cgaatttatt
      241 gtgcagctcg gcccaagcgt cgaccgtgcc cggcacgctg atcggcaaca tgccgaacgg
      301 cgggatcgtc gtgcgatgta gcttgtcgag ctcggcttta atttgctcgt aggtaaggcc
      361 tagcggcgaa cgtccgctgc cgttgatctc gtaaagcttg ttctccttcg cgctgtaaac
      421 gatggcgaaa aggtctcctc cgattccgtt cgacaccggc tccatcaatc cgagcgccgc
      481 gttcgccgcg atcgccgcgt ccactgcgct gccgccacgt ttcagaatgt cgattcccac
      541 ctgggtggcg agcggcacac ttgtgcaaac cattccatgt cgcgcgacga cctccgagcg
      601 ggtcgcgaaa ttttttccgg tgatgcgatc gatctgcgcg tgcccgctaa gatcgataat
      661 gaagaaaaga ccaagcgaaa gagtgattcg gcgcatcccg gaggataacg cccaagaatt
      721 ccatcgacaa agcctaacga atttggctac aacgcgccca tgaactggaa aaaattcttt
      781 atcgcgctcg tcgctgcctt tgtttttatc ttcctgttcg ggttcatctg gtatggcatg
      841 ctgatgaatc cgattcatcg cgaagtgccg gcgctctttc ggcccgaagc agatttccgc
      901 agttatttcg gctggctaat attcggccac ctggtgatgg cgttcttcct cacgctactt
      961 tgcgcccgtt acactccggc cggcggcgca ggcggcgggg cgcggcttgg cttcatgacc
     1021 gcgttgattt atgccggcgc cgatttcatc acctacgccg tgcaacccct gacaacgaaa
     1081 atgctcgccg gttggatcgt cggcgatttg ctcatgttcg cgatcgccgg cgcaattatc
     1141 ggtgcgatct acagacccgg tccgctcacg agcggggcca acgtttagcg gatgcgctat
     1201 tcgcgccgct gtttttcgac gagatggcgc aactggcgtt ccatcgtctt gaacgcgtcg
     1261 tggatcactt tggccagcgg ctcgtgcgaa ccgttgtcca tctgtttttc atcggcgacg
     1321 agctcgtgtc gcggcgcgac cgtaacgtcg atgcggcagc ggtaaggatt gcccttttga
     1381 tgggtgcgat tggtttgctc aatcgcgacg tcgcatcggt tgatgtggtc gcagaacttt
     1441 tccaagcgcg cggcgtaatc catcaccaga ttgtcgatct tgtctgattt ttccaggcca
     1501 cggtaagtga tgttgactgg cagttgcatg accaaatatg gtaactcaaa tcgggcgaag
     1561 tgaacgaaaa tgtgccggca attttgccgg ctcgatcttc accaggtcgg atttgacgtg
     1621 ctcgacgatc cggtcgcgca cgcgagcggg taaaagatcg acgattgctg aatgcatttc
     1681 cgcgggcgcg gcgaacgacc acccttcctt gccattctcg gtgacgatct tggcgatttc
     1741 gtcggcgagc tgtttgtaga tgcgccggtc ggtctcgttt tcgagctgtg tccgctcggc
     1801 gatggacgcg gcgccacgat gcgagccggc ggcgtccgtc acggggaagc gcccggccaa
     1861 atcgctcacc ttgtcgatca gtttgccgtg cgcatcggtg aggttgaacg cctgcaccag
     1921 ctgcaagctc gggccgcggg ttggcgtttc atcaacccgg tacgctttca gacttccccg
     1981 atcagcgaca atgatgaaag aggtcggaat cataagcgaa tggtggagtg acaaacttca
     2041 gtcggaaacc gcctcgcatg aagctcgttt gaaatgtcta cagtaaaatt cgtttcccag
     2101 cagaacgttg cgcttctcgc aacgcgcggc gctattcggg cgccttcctc ccaccggcgc
     2161 gctcctcttg ggcactaaac gcttcgggta aaccgatctc gcgtttcttc cacgactcct
     2221 ggcgcgcctg cacttgcaag ccgcgcgtca accggaacaa acacgcgctg cgcccggcaa
     2281 gatcggcatc gtcgccagag gaacacttgc gcggctcggc aatgaagccg tcctcgttcg
     2341 cggtgtcgag gatcagttcc cactcgagat tttcttcacc cggcagcatg aatggcatca
     2401 cgtcataatg cccgttgatc aaaaggagaa aagtgtcgtc acgcacgggc tcgccttggg
     2461 agtccaccac atcggtcgca tcgccgctca acagcatgcc gagacaacgg acgaaggctg
     2521 atgaccattc ctcctgtttc atttcgttgc cgccgggatt gaaccacatc acatccttga
     2581 tctcgctgcc tcgaatccgc cgcccgcgga aaaatttcgg gcgccgaaac actggatgcc
     2641 gcttgcgcag ctggatgagc tttttcgtga actcaagcag ccggagttgc tcatcatcgc
     2701 gctgccagtt aaaccagctg atctcgttgt cctggcaata ggcgttgttg ttcccgtttt
     2761 gggtgcgacc aaattcatcg ccgccgagca gcatcggcac gccctgtgat aagaacagcg
     2821 tcatcaggaa attacggcgc tgtttccgcc gcagcgcatt gattttcgcg tcgtcactcg
     2881 gcccttcgac gccgcaattc caggattgat tgttgtggtc accgtcctgg ttgttttcgc
     2941 cgttggcttc attgtgtttc tcgttgtaag tgaccagatc gttcagcgtg aatccatcgt
     3001 gcgtagtgac gacgttgacg ctcgcatacg gccggcgatc atcgtgctga tataagtccg
     3061 ggctgccggt tagccgatac gccacttcgc caatcttctc ctcgtcgctt ttccagtaac
     3121 tgcggaccgc gtcgcgaaat ttcccgttcc actccgacca gccggtcggg aaattgccga
     3181 cctgatagcc gccgtcgccc acgtcccacg gctcagcgat caatttcact cgtgagagga
     3241 ccgggtcctg ttgaatcgct ttgaagaaag catggaactt gttgacgccg gcgccgtcgc
     3301 gcgccagcgc cggggcgaga tcgaaacgga agccatcaac gcgcatctcg ctcgcccagt
     3361 agcgcaggct gtccataacc aactgcaacg tgtgcgggtc gagcgtgtcg agcgtgttac
     3421 cggtgccagt gtaatcgaga taaaatcgcg ggttatcggc caacagccgg tacgacgcga
     3481 tgttatcgat gccgcggaaa caaagcgtcg ggccgagatg attgccttcg gcggtgtgat
     3541 tgtaaacgac atcgagaatg acttcgagcc cggccccgtg cagcttgcgc accatggctt
     3601 taaattcgcc aacctgctcg cccatttggc cgctgctcga gtactcgcca tgcggcgcga
     3661 aaaagccgat cgtgttgtaa ccccaataat ttgttaggcc gcgatccgcc agaatcttgt
     3721 cgtcaatgtg cgcgtgcacg ggaagaagct cgaccgccgt gatgccgagg tttttcagat
     3781 acgcaagtgc cgcgtcgcag gccagaccag cataagtgcc gcgcagattt tccggcacgc
     3841 ccggacacaa ctgcgtgaat cccttcacgt gcacttcgta gatgatcgac tcagccagtg
     3901 gcgtgtcggg ccgtttgtcg tcaccccagt cgaaggcaag atcgacaacc actgccttcg
     3961 gcatccccca ggcgttatcc cgaaaatcgc agctaaggtc ttccagttcg tcgccgacca
     4021 cgtagccgaa catctcatcc gcccaattta tctcgcccgc gatggctttg gcatacggat
     4081 cgaggagcaa cttcgagctg ttgaaacgca gcccgcgctc cggctcgtac gatccggcaa
     4141 cgcgaaagcc gtacagttgt cccggccggg catcgggcaa aaagacatgc cagatataat
     4201 tcgtgcgttc ggtcaccggg acacgaatgt tttcttcggt cgcgtcgagg ccgtcgaaca
     4261 ggcaaagctc gacggcggtc gcccgctgcg agaagagcgc gaaattgacg ccgttgccga
     4321 gccaggtcgc gcccagtgga tatggtcggc ccggccatgc tttgatcggc gtcataacgt
     4381 ttcctctgcg aaatcttcgg ggccggctga ttgccagccg cag
//
LOCUS       DASTDG010000209        16383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_491920, whole genome shotgun sequence.
ACCESSION   DASTDG010000209 DASTDG010000000
VERSION     DASTDG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491920"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFO30_06840"
     CDS             complement(<1..268)
                     /locus_tag="VFO30_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9419041.1"
                     /translation="MQRPEQRDEVEMLALMLLIRRFEERASQQYQAQKIGGFCHLYIG
                     QEAVVAGAVAAVRDDDYIITAYRDHGHALARGTSANACMAELFGK"
     gene            complement(275..922)
                     /locus_tag="VFO30_06845"
     CDS             complement(275..922)
                     /locus_tag="VFO30_06845"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HET9419042.1"
                     /translation="MKRSQMRLCISRQTTKFLPMIPAIVLMLVVLAYRITTGLMIVHS
                     GATWLSNFVPFAALALCGAAYFPNKLKFALPLGALFVSDVILNYSYGASLIDPHTASR
                     YFALTVVGLLGFVLRKHASLKTLLPASLVGSAIFYLVTNVFAWLSDPGYAKTSAALVQ
                     ALTVGLPQYSATPAWMFFRNSLISDLLFTLLFVVCMNLGRNTAPVRAAATVARPA"
     gene            complement(1020..1274)
                     /gene="rpsT"
                     /locus_tag="VFO30_06850"
     CDS             complement(1020..1274)
                     /gene="rpsT"
                     /locus_tag="VFO30_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9419043.1"
                     /translation="MANTKSAAKRARQSATRALRNRSVRSQLRTLQRRVRAAAEPAAD
                     EIPNLFSAIDKAAKRSIIHHNAANRRKARLNKLLTGTAKK"
     gene            complement(1352..2026)
                     /locus_tag="VFO30_06855"
     CDS             complement(1352..2026)
                     /locus_tag="VFO30_06855"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9419044.1"
                     /translation="MFRPLTVAVCLFIAPAWHAFAQQDAADRVIVSVRDQKLMLVQDG
                     FAVATYPVSTSKYGLGDRWGSLATPLGFLQVAQKIGDHAPTGAVFHNRRWTGEILAPN
                     VPGRDPITTRIIWLRGLEAANAHAFSRCIYIHGTPEEKIIGRPASYGCIRMKSSDVTV
                     LYDRLAIGALVQIVPDGLPKISKAPRNFQPMNQQPVVSSETKAGSVPQAKARSVPLLA
                     SSRGGA"
     gene            complement(2058..3563)
                     /locus_tag="VFO30_06860"
     CDS             complement(2058..3563)
                     /locus_tag="VFO30_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HET9419045.1"
                     /translation="MQGSPGHLIWTICYLTVLLGLSAYGVHRYFIIYLFLKHRKRAPL
                     PAGIFEQLPKVTMQLPIFNEVYVVERLLRSVSELDYPRELLQIQVLDDSTDDTREMTA
                     QLVHELRRRGFDVQLIHRLDRTGFKAGALAVGLDSADGEFLCILDADFVPQPDLLKRT
                     VHFFTDPKIGMIQTRWGHLNRGYSLLTRVQAMFLDGHLLLEQTARSRSGRFFNFNGTA
                     GLWRRSCIEEAGGWQHDTLCEDLDLSYRAQLAGWRFIFLPDVVTPAELPVDMNGFKSQ
                     QHRWTKGSIQTCKKLLPTIWRSKLPFPIKVEATGHLMSNFAYLLLACLCVLLHPSVGG
                     PQGGWVRTLLIDGPIFIAASVSVAVFYICAQRELHPRTWVKEILFLPCLLALGIGLSL
                     NNARAVLEALVNHKSDFTRTPKYGIERKSQPWRTCKYKPLHSMLPIAEMAFAIYFTYF
                     VGFAIVHRQFLSVPFLLMFQGGFLYVAVSSLLAWWPKFTFGGARHASPVPA"
     gene            3852..6329
                     /locus_tag="VFO30_06865"
     CDS             3852..6329
                     /locus_tag="VFO30_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9419046.1"
                     /translation="MQRTRDRSREFNTVFPILLAGWVPAVLTLFVSYTILSNTLESKI
                     LRDRQTFVQLIAHLLGDDFAGKRSLIEYYQTLPEVAQTLTAPDATIIGQQWLNQAFFS
                     HPRIDGMFLTDAEGRLIASVPALPALIGQDFGSNLWREGALATSGAFVSPVHPRQADQ
                     RLATDIVGVVRTPDDRLVGYLGVSVLVERMGRRLSAIEFADESLCEILDQKGTAIFAK
                     TMQPTSNPVSADAAMLMKEISRSKAGHFEHSGNIYSFTPVESTGWVAVVEQPKVVAYK
                     PVYDLISRMTLLAVWLVVLTTVFAWLGNKFQRRQIEASRRIEREVIFNEKILANVPSG
                     IAFVDPVSRHFLQANEAFTQMARRFGNLPPERDITEATYTDISIAPVDALEKVLAFGT
                     PFQLIEQPFKNETGMTHFLNIHLLRLQGPNQTVQGVLYLVEDKTRDVTLRQELIDANA
                     AKDQFLALLSHELRNPLTPVIPMVEELEANMGDSASNRQALEVIRRNVELEARLIDDL
                     LDVTRIAKGKLQLTFEVVCVPEILHRAYEICREEIASKNLKVDFELRANRSHLEGDPA
                     RLQQVFWNLIKNSVKFTPENGRISIETLNPTAHDIEIRVTDTGIGIEQEKMDKIFNAF
                     EQGQTSITRRFGGLGLGLAISKAMVDAHGGKIRVESRGKDQGATFAITLRTVPEPAAK
                     AGGDGHTAAPPKQRRQRTAGRRVLVVDDHEDTCLGMKLMLERRGYRITLAHSAEQALE
                     KAAQEHFDLLISDIGLPDRSGYELMRELGTSKGLRGIALSGFGMEHDVSKARQAGFSQ
                     HLTKPINFDRLEEAIQNLLELEPSSKS"
     gene            complement(6361..6714)
                     /locus_tag="VFO30_06870"
     CDS             complement(6361..6714)
                     /locus_tag="VFO30_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9419047.1"
                     /translation="MAKLTKRDIVVAISNQTGMVQHEVFDVVQRTLDKITDSLANNIP
                     VELRNFGIFQPRLTKPRVGRNPNQPGSSFVIPPRATVKFKAGKVMRQRVEKLSRELKE
                     ASEQKQAETANGRVQ"
     gene            complement(6826..7260)
                     /locus_tag="VFO30_06875"
     CDS             complement(6826..7260)
                     /locus_tag="VFO30_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419048.1"
                     /translation="MRDQALERKLRLLNIQLENWKKLHDLITYGLDRAKPIISTEQEH
                     QFTELRANLLQETEHILGELKILGELSGKTMNVLQRAISVRAVRELPNDEVRRLESEW
                     NAVFTKLGVVQGQLKAIRKKLAGQTPLGYFFERLVRRPATAH"
     gene            complement(7270..7998)
                     /locus_tag="VFO30_06880"
     CDS             complement(7270..7998)
                     /locus_tag="VFO30_06880"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9419049.1"
                     /translation="METRVLILNADPVFEDKALTVLETGADIDRQIVRTLAEAVAVLL
                     GENFDAFLIEGEAALALEQATNARQHFPSLKIVCLLNDRPEKEMLAAAKQQNLELIAS
                     PQRDGRLKRAIARSLRSVEKATAPASDGIDPCHSLIGNLNQFSAAEILQMSCLSQRSG
                     RFTFKSRRGNSEIYLQHGAIRHAMFGSLDGEAAVAEIFRWRQGRFYFEEGIISQVQTV
                     NRPWAHLLIDNLQKLDETLELASV"
     gene            complement(8065..9009)
                     /gene="rnhC"
                     /locus_tag="VFO30_06885"
     CDS             complement(8065..9009)
                     /gene="rnhC"
                     /locus_tag="VFO30_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET9419050.1"
                     /translation="MNSFTALVTTQQVDKLRALLHELGFEFSPKEYTVFFAQKNKLSV
                     AVYEKGPKVLVQGKGAEEFVQFELEPKILGEARLGYEEVHSPEMFQPHFGVDESGKGD
                     FFGPLVIAGVYVDLGIARKLRDAGVVDSKRIGSDARIRDLAKTIRKSTAGSHETVLIG
                     PAKYNELYAKFANLNALLGWGHARVIENLLAKKPECPRSLSDQFADARVIENALLRHG
                     RKIEIEQRPRAESDIAVAAASILAREAFIDWLNRRGKTLGVRLGRGVSSNVKETAKQL
                     VERQGPHILREVAKVHFRTAHEIAPQAYANPPQRVWPR"
     gene            9085..10197
                     /locus_tag="VFO30_06890"
     CDS             9085..10197
                     /locus_tag="VFO30_06890"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9419051.1"
                     /translation="MAIRAVQFRHALLRWYRKHGRDLPWRQTRNPYAVLVSEFMLQQT
                     QVAAVLPFYNEWLRRFPDFAALARASQKDVLHAWQGLGYYARARHLHATAKLVQDRHR
                     DGFPSEIDSMRELPGVGRYIAHATATFAFDQAVPVVEANTSRVLTRLFDLRLPIDHTA
                     GRNALWQYARALLPPTGARIYNSALMDLGALVCLRRPKCAICAVKTFCRANRPETLPV
                     KRARPPIAYLVENHALVSRRNKILLQEAQHRWRGMWILPRLALDGFKPPSLQPNEVYT
                     GVFPFTHHRITLKVFVQRKRKIDSYLGRWFNLNALDTIPIPTPHRRAIISLLKPEGSH
                     TLRLKEHSPAITEDSDEKHDRLRSRRGRAQRPSVLC"
     gene            10142..11008
                     /locus_tag="VFO30_06895"
     CDS             10142..11008
                     /locus_tag="VFO30_06895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET9419052.1"
                     /translation="MTGYGRGEAEHNGHRFCVELNSVNRKQSDVVVNLPRDLTALEPR
                     IRETINENISRGRTNVTVTLRNRANGAQQLALDTNLARFYHQAMRALQQELNAPGEIT
                     IGLILQAPGVMRLPEQVVDPDEVWPRVEQALRAALINLIKMREREGKHLAKDLIHRLK
                     QIRKKLKEIRGLHPDVTKKYRTALLDRLEKAGLPLDPADERIVKEISFFADRTDVSEE
                     LTRLESHLAQFAHHLRRNEPVGRTLEFIAQEISRELNTLGAKGNDAAISQRVVACKAE
                     LEKIREQVQNLE"
     gene            11096..11710
                     /gene="gmk"
                     /locus_tag="VFO30_06900"
     CDS             11096..11710
                     /gene="gmk"
                     /locus_tag="VFO30_06900"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9419053.1"
                     /translation="MSSSEFSRYGILFVISAPSGAGKTTLVEALRQTANLFYSVSCTT
                     RAPRGGEMDGEDYRFLSDAEFQARVKADEFLEHAQVHGDHYGTLRDPVVANLKKGVDV
                     LIDIDTQGAATIRSCDDPFIRAALTDIFIMPPDLDELRRRLLKRGTETTEKIELRLAN
                     AAREMQFWRDYRYTVISKSMEEDLQKLRHIIAAESFLSRRLIRK"
     gene            11736..12359
                     /locus_tag="VFO30_06905"
     CDS             11736..12359
                     /locus_tag="VFO30_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET9419054.1"
                     /translation="MKPDSKSRADKISARKVVRLRAGKRTVVLGVTGSIAAYKSAELA
                     SLLVKQGHDVFVVMTHHATEFIAPLTLQTLSKNPVMTSFFDEKENWRPGHIELADRAN
                     LLLIAPATAHIIAELAHGLANHPLAAIALATRAPILLAPAMNGKMWDHPATQENVAKL
                     KSRGVELIGPEAGMLACGYEGLGRLWKVDDIAFRAEFMLARQENLIA"
     gene            12356..13006
                     /locus_tag="VFO30_06910"
     CDS             12356..13006
                     /locus_tag="VFO30_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET9419055.1"
                     /translation="MRFVVTAGPTREPIDPVRYISNRSSGKMGYAIVEAAITTGHDVT
                     LISGPVHFDQPHGAHVVSVMTSDEMFYAVHQHLRDCDILVMCAAVADYKPAIVAPRKI
                     KKRDASLTLELVPTRDILTSIPHDREFLVIGFAAETDELEKKAAQKLRAKNCDIIVAN
                     DVSRADAGIESDENAVTLLFRGGKTKKISRAPKKIIARELVEIFLNAREKRLTTKT"
     gene            13330..14088
                     /locus_tag="VFO30_06915"
     CDS             13330..14088
                     /locus_tag="VFO30_06915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9419056.1"
                     /translation="MKHYLEAAENAARAAGDLLRENFQPRKHSGLRVNSAEAHDIKLE
                     IDVRAQELITSSLLKNFPQHALYGEEGVVGDQTSEHQWVVDPLDGTVNFYYRIPHFCV
                     SIALRFKGEIIVGVIYDPIRDEMWSGQKGEKPRLNGQHFRVSERTELAEAVISVGLSK
                     TGVLIESNVPMLHQMIHRARKCRLLGSAALDMAYVACGRLDAYIEQGINLWDIAAGWI
                     LVETAGGSVDLRPRKDMKDKYSIIASNGVIDLKL"
     gene            14085..14567
                     /gene="ispF"
                     /locus_tag="VFO30_06920"
     CDS             14085..14567
                     /gene="ispF"
                     /locus_tag="VFO30_06920"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9419057.1"
                     /translation="MSTTRCGIGYDIHRFAEDRQLILGGVEIANTRGLAGHSDADVLS
                     HAIADALLGAIGAGDIGQHFPNTDEALRGISSIEILRRISALLAEKKARVVNIDATII
                     AEAPKIAPHVSAMRKDIAAAIGVNGANISIKATTNEMLGAIGRGEGIAAMAIATVEQE
                     "
     gene            14695..16146
                     /gene="cysS"
                     /locus_tag="VFO30_06925"
     CDS             14695..16146
                     /gene="cysS"
                     /locus_tag="VFO30_06925"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9419058.1"
                     /translation="MALRFFNTYSRQLEEFTPRDPSSHSPPDEALRPADAIDMYTCGP
                     TVYSRAHIGNFRAYVFEDLLQRHLESRGYQVHRVMNITDVDDKTIRGAREAGVPLQKF
                     TAQFKDAFFQDIDTLRIKRADQFPAATDQRYIDKMIAMIKTLIDRGLAYLADDKSVYY
                     RINKFPNYGKLAHFDLTQLQSTGRVKHDEYDKEHIGDFALWKAWDEEDGEVKWDSPWG
                     PGRPGWHIECSAMAMALLGDQIDIHCGGIDNIFPHHEAEIAQSEGVTGRQFVRYWLHC
                     AHLLVDGQKMSKSLGNFYTLPDVLAKGYTGREVRYALMRVHYRAPLNFTWEGMKEARE
                     SLGRIDDWLQRLRECSASVSLAKTDKGQAGSLPCTFEDALDDDLNISGALGFLFESIR
                     ETNRALDHKELDSSAAEEWLSWWQRINRVLNVEKHDGAAPPEIAKLVEARAQARLAKD
                     FRKSDELRDELSARGWEVRDTKDGPKLTRRGGA"
     gene            16157..>16383
                     /locus_tag="VFO30_06930"
     CDS             16157..>16383
                     /locus_tag="VFO30_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HET9419059.1"
                     /translation="MFAPAQFFSLDHTAHPKLFENQNYVWDALKQIASYLQFRLKPAI
                     LGDLVGKPFISNHVFIGRGTIVEQGAVLKGPA"
BASE COUNT         3687 a         4684 c         4702 g         3310 t
ORIGIN      
        1 cttttccaaa cagctcggcc atacacgcgt tggcgctcgt gccgcgggcg agcgcgtggc
       61 cgtggtcgcg gtaggcagtg atgatgtaat cgtcgtcgcg caccgctgcg accgcgccgg
      121 caaccaccgc ttcctgtcca atgtaaagat ggcagaagcc gccgatcttc tgcgcctgat
      181 attgctggct cgcgcgctcc tcgaaacggc ggatcagcag catcagcgcg agcatctcga
      241 cctcgtcgcg ttgctccggc cgctgcatcg ggagttatgc ggggcgcgcg accgtcgccg
      301 ccgcgcgtac aggcgcggtg tttcggccca agttcatgca aacgacaaaa agcaaagtaa
      361 agagaagatc gctgatgagc gaatttcgga agaacatcca ggccggcgta gcgctgtatt
      421 gcggcaggcc aaccgtgagc gcctgaacta aagcggcgga ggtttttgcg taaccggggt
      481 cgctcagcca ggcaaagacg ttcgtgacga ggtaaaagat tgctgatcca acgagcgacg
      541 ccggcaggag cgtcttgagc gaagcgtgct tgcgcagcac gaacccgagt agtccgacga
      601 ccgtgagagc gaagtagcgc gaagcggtat gcggatcgat gagcgaagcg ccgtagctgt
      661 aatttaagat gacgtcggag acaaaaagag caccgagcgg aagcgcgaac ttcaatttgt
      721 tcggaaaata agcggcgccg cagagggcca acgcggcaaa gggcacgaaa ttggagagcc
      781 aagtcgcgcc ggaatggaca atcatcaggc cggtggtgat gcggtaggcg aggaccacga
      841 gcattagaac aatggcggga atcatcggca gaaacttagt cgtctgccgc gatatgcaaa
      901 gacgcatttg ggaacgtttc atggccccgc gatggccggg tcctcctctc cctcaagcga
      961 aaggactgag gtgatggcaa accgttctag acctggaacg cagcaattct gcgcccgaat
     1021 tactttttcg ccgtgccggt aagcagtttg ttcaggcgcg ctttgcggcg attggcggcg
     1081 ttatgatgga tgatgctgcg cttggcagcc ttgtcgatcg ccgaaaagag gttggggatt
     1141 tcatcggccg ccggttccgc tgcggcgcgg acgcggcgct gtaatgtgcg cagttgggag
     1201 cgtacgctgc gattgcgaag agcgcgtgtc gcactttgcc gggcgcgttt agctgccgat
     1261 tttgtgttcg ccatcgctgg aaaatttgcg ggctggaggc tcgcgggtag ccactgcgtt
     1321 gtcaaatgtc gatctggcgg ccgcgcggtg attacgcccc gccgcgggac gaggcgagca
     1381 gtggaactga gcgggctttc gcttgcggca cgctgccggc ctttgtctca ctggagacaa
     1441 ccggctgctg gttcattggc tgaaaattgc gcggtgcttt tgagatcttt ggcagaccat
     1501 cgggcacgat ttgaacgagc gcgccaatgg ccagccggtc gtaaagaacg gtcacgtcgc
     1561 tggatttcat ccggatgcag ccgtagctcg cgggccgccc gatgattttc tcctctggcg
     1621 tgccatggat gtaaatgcac cgactgaagg cgtgcgcgtt ggccgcttcg agcccacgca
     1681 accagatgat ccgagtggtg ataggatcgc ggcccgggac attgggcgcg aggatctcgc
     1741 cagtccagcg gcggttgtgg aaaacagcgc cggtcggcgc gtggtcgccg attttttgcg
     1801 cgacctgcaa aaagccaagc ggcgtggcca ggctgcccca gcgatcgccc agcccgtatt
     1861 tagaagttga gacggggtag gtcgcaacgg caaagccatc ttgcacgagc atcagctttt
     1921 gatcgcggac gctaacgatg acgcggtcgg cagcatcctg ttgagcgaag gcatgccagg
     1981 ctggggcaat gaacaggcaa acagctacgg tcaacgggcg gaacatacgc gggtttttag
     2041 cagaaaacgt gcgtttgtca cgcaggaact ggggaagcat ggcgggcccc gccgaacgtg
     2101 aactttggcc accacgcgag cagtgatgac acggcaacgt agaggaatcc gccttgaaac
     2161 atcagcagaa aaggcaccga cagaaattgt cgatgcacga tggcgaagcc gacgaagtag
     2221 gtgaagtaaa tcgcaaatgc catttcggcg atgggcagca tcgagtgaag cggcttgtat
     2281 ttgcacgtac gccacggctg cgatttccgt tcgatcccgt acttcggcgt gcgggtgaaa
     2341 tcggacttat gattgaccaa cgcttccaaa acggcgcgcg cattgttgag cgacaaacca
     2401 atgccgaggg cgagcaggca gggcagaaac aggatctctt tcacccaagt gcgcggatgc
     2461 agctcgcgtt gcgcgcaaat ataaaacacc gccaccgata cggaggcggc aatgaagatg
     2521 ggcccgtcga ttaacaaagt gcggacccag cccccctgcg gaccgccgac agagggatgc
     2581 agcagcacgc aaagacacgc aagcaggaga taggcaaagt tcgacatgag gtgacctgtc
     2641 gcttcgactt tgatcgggaa aggcaacttg ctgcgccaaa tggtgggcaa tagttttttg
     2701 caagtctgga tcgaaccttt cgtccaacgg tgctgctggg atttgaagcc attcatgtcg
     2761 accggcaact cggccggcgt gactacatcg ggcaagaaga tgaagcgcca gccggcgagc
     2821 tgtgcgcgat aactcagatc gagatcttcg cacagcgtgt cgtgctgcca gccgccagcc
     2881 tcctcgatgc aggaccgacg ccacagaccg gcggtgccat tgaagttgaa gaatcgaccg
     2941 ctgcgactgc gcgccgtttg ctccaaaagc aaatgtccgt cgaggaacat cgcttgcacc
     3001 cgcgtcagca gcgaatagcc gcgattaagg tgaccccagc gtgtctggat catgcctatc
     3061 ttcggatcgg tgaagaagtg aactgtgcgc ttgagcaggt ccggctgcgg cacgaagtcc
     3121 gcatctaaaa tgcagagaaa ttcgccatcg gccgaatcga ggccgacggc gagcgcgccg
     3181 gctttgaagc ccgtccgatc gagacggtga atgagctgta cgtcaaagcc gcggcggcgt
     3241 agttcgtgca ccagctgcgc ggtcatctca cgcgtgtcgt cggtggaatc gtccagcacc
     3301 tggatttgga gaagttcgcg cgggtagtcg agttcgctca ccgagcgcag cagacgttcg
     3361 actacataga cttcattaaa gatcggcagc tgcatcgtga ccttcggcag ttgttcgaag
     3421 ataccggccg ggagtggcgc gcgcttccga tgcttgagga agaggtaaat tataaagtaa
     3481 cggtgaacgc cgtaagccga caggccgagc agaacggtca aatagcaaat cgtccaaatc
     3541 aaatgaccgg ggcttccttg catgggtcta ccgcaagctg cgtgtggata aaggcagcaa
     3601 gggcgacatg atgccgcttc cggcgtgggc gtcaaattaa aaaatgccgg tttgccttgg
     3661 caaacctgac gaaattgcgc cactcaactc gccgcgaacg cgcccgtcca ttgttgttcc
     3721 agtcgttgtc accgttgcgg tcggccaaat gagatgtcga tctaacgcgg taagtggtaa
     3781 cgcgacggca aaacccttgc tgatatgcta tgttgctcac ccccaatcgt atgcagcggc
     3841 gagacagttt agtgcagcgg acacgcgatc gctcccgcga gtttaacacg gtcttcccta
     3901 tccttttggc cggttgggtt ccagcagtcc tgacgctgtt cgtttcctac acgattcttt
     3961 cgaacacgct tgaatcgaaa attctgcgcg accggcagac gttcgtgcag ttgatcgcgc
     4021 atttgctcgg ggacgatttc gccggcaaac gtagtctgat cgaatactac cagacgcttc
     4081 cggaggtcgc acaaactctc actgcaccag acgccacgat catcgggcag cagtggttga
     4141 atcaggcgtt cttctcgcac ccgcgaatcg atggcatgtt tttgactgat gccgaggggc
     4201 gcctgatcgc gtcggtgccg gctttgcccg cgctgatcgg ccaggacttc ggctcgaact
     4261 tgtggcgtga gggcgcgctc gccacctccg gcgcattcgt ttccccggtg catccacgcc
     4321 aggctgatca acggctcgcc accgacattg taggcgtggt gcgcacgcct gacgacaggt
     4381 tggtcggcta tctgggcgtg tcggtcctgg tggagcgcat gggccggcgc ctgtccgcaa
     4441 ttgagtttgc cgacgagtcg ctctgcgaaa ttctcgatca aaaaggcacc gccatcttcg
     4501 ccaagacaat gcagccaact tcaaacccgg tttcggctga cgccgcgatg ctgatgaaag
     4561 aaattagccg aagcaaggcc ggccatttcg agcatagcgg caacatttat tcgtttaccc
     4621 cggtggaatc gaccggctgg gtagcggtgg tcgaacagcc gaaggttgtc gcttacaaac
     4681 cagtctacga tctgatctcg cgcatgaccc tgctcgcagt ctggctggtc gtattgacaa
     4741 ctgtgtttgc gtggcttgga aataaatttc agcggcgaca gatcgaagca agccgccgca
     4801 tcgagcgcga ggtcatattc aacgaaaaaa ttttggccaa cgtgccgagc ggcatcgcct
     4861 ttgtcgatcc ggtgtcgcgt cattttctgc aggcaaacga agcgtttaca caaatggctc
     4921 gccgcttcgg caacttaccg cccgagcgcg acatcaccga ggccacttac actgacatca
     4981 gcatcgcgcc ggtggatgcg ctcgagaaag ttctcgcgtt cggcacgcca ttccagttga
     5041 tcgaacagcc gttcaagaat gagacgggga tgacgcactt tttgaacatc catttgctgc
     5101 gattgcaggg gccgaaccaa accgtgcaag gcgtgcttta ccttgtcgaa gacaaaacgc
     5161 gcgacgtcac gctgcggcag gagctaatcg acgccaatgc ggccaaggac cagtttctgg
     5221 cgctgctatc gcatgagctg cgtaacccgc ttacgccggt aattccgatg gtagaggaac
     5281 tggaagcgaa catgggcgat tctgcgtcca atcgccaggc gctggaggtg attcggcgca
     5341 acgtcgaact cgaagcgcgc ctcatcgatg acctgctcga tgtgacccgt attgccaagg
     5401 ggaagttgca gctcaccttc gaagtggtgt gtgtgccgga gatcctgcac cgcgcctatg
     5461 agatttgccg ggaggagatc gcgagcaaga acctcaaagt cgactttgaa cttcgtgcca
     5521 atcgcagcca tctagagggg gatcccgcac gcctgcagca ggtcttttgg aacctaatca
     5581 aaaacagcgt caaattcaca ccggagaacg gccgtatctc tattgagaca ctcaatccca
     5641 ccgctcacga catcgagatt cgagttaccg acactggcat cgggattgag caggaaaaga
     5701 tggacaagat cttcaacgcc tttgagcagg gccaaacatc cattacccgc cgatttggcg
     5761 gccttgggct cggccttgcc atttccaagg caatggtcga tgcgcacggg gggaaaattc
     5821 gcgttgaaag tcgcgggaag gatcagggcg caacattcgc gatcacatta cgaactgtac
     5881 ctgaaccagc ggccaaggcc ggtggcgacg gccataccgc tgcgccgccg aagcagaggc
     5941 gacagagaac agcaggccgc cgcgtcctcg tggtcgacga ccatgaggat acgtgtcttg
     6001 ggatgaaatt gatgctcgag cggcgcggct accgaattac gcttgcgcat tcagccgagc
     6061 aagcactgga gaaagccgcg caggaacatt tcgatttgct catcagtgat atcggcttgc
     6121 ccgatcgaag tggctacgaa ttgatgcggg aactcgggac aagcaaaggg ctgcgcggca
     6181 tcgcgctgag tggctttggt atggagcacg acgtcagcaa ggcgcgtcag gcaggatttt
     6241 cccagcacct gaccaagccg atcaatttcg atcgcctgga agaagcgatt cagaatctgc
     6301 tcgagcttga gccgagctcg aaatcgtagg ccatcagcag ccgcggagga ctgacacaat
     6361 ttactggacg cgcccgtttg ccgtctcagc ctgcttctgc tcggatgcct ctttcagttc
     6421 gcgggagagt ttttccaccc gctggcgcat gactttgcca gctttgaatt tgaccgtggc
     6481 ccgcggcgga atgacaaaac tgctgccggg ttgattcgga ttcctgccca cccgtggctt
     6541 ggttaaacgc ggctgaaaga tgccgaaatt gcgcaactcg acagggatgt tgttggccaa
     6601 actgtcggtg atcttgtcta acgtgcgctg caccacgtcg aaaacttcgt gctgcaccat
     6661 cccggtttga ttgctgatcg ccacgacaat gtcgcgtttc gtcagttttg ccatactaat
     6721 atcagtatcg cttggcatct tgaaagtggc ggcaaaatta acagcgaccg gagaatccgg
     6781 ccgcgtaaga gcctcgctgc caaccgacaa cggtgtcggc gcctgctaat gcgcggtcgc
     6841 cggccggcgg acaaggcgct cgaaaaaata ccccagtgga gtctggccgg ccaacttttt
     6901 gcggatcgcc ttgagttgac cttgcacgac accaagtttg gtgaagaccg cgttccactc
     6961 gctctcgagc cggcgcactt catcgtttgg cagctcgcgc acggcccgca ctgagatggc
     7021 gcgctgcaaa acgttcatcg ttttgccgga caattcgccg aggattttca gctcgccgag
     7081 aatgtgctcg gtttcctgca atagattcgc gcgaagctcg gtgaactgat gctcctgctc
     7141 tgttgaaata atcggcttcg ccctgtccag gccgtaggtg ataaggtcgt gaagcttctt
     7201 ccagttttcc agctggatgt tgagcaaacg aagtttccgt tcgagggctt gatctctcat
     7261 gcgcggaagc tagacgctgg ccagctcgag cgtttcatcg agcttttgca aattgtcgat
     7321 caacaggtgc gcccatggcc ggttaaccgt ctgcacttgg ctgatgattc cttcctcgaa
     7381 atagaagcgc ccctgccgcc aacgaaagat ctcagccact gccgcctccc catcgagcga
     7441 accaaacatg gcatggcgga tcgcgccgtg ttggagataa atctcactgt tcccacgacg
     7501 cgacttgaac gtgaagcgcc cgctgcgctg gctcaaacaa ctcatctgta gaatttcagc
     7561 cgcggaaaac tggttcagat tgccgatcaa agagtgacac gggtcgattc cgtccgatgc
     7621 cggtgcggtc gctttttcaa ccgagcgtaa cgagcgcgcg atcgcacgct ttaagcgccc
     7681 atctcgctga ggcgacgcaa tgagctcgag gttctgctgt tttgccgctg cgagcatctc
     7741 tttctctggc cgatcattaa gcaggcaaac gattttcagc gatggaaaat gctgccgggc
     7801 attagtcgcc tgctccagtg ccaatgctgc ttctccctca atgaggaaag cgtcgaagtt
     7861 ttcacccaat aagaccgcca cagcctcggc gagcgtgcgc acgatttgcc tgtctatgtc
     7921 ggccccagtc tcgagaacag tcagagcttt gtcctcgaag acaggatcgg cgtttaagat
     7981 gagaaccctc gtttccacgt tctgccggtg ttgttttcag agcaggaatc ttgccggaca
     8041 ccccagcgaa atcaccgccg cggcttatcg cggccagacg cgttgcgggg ggttagcgta
     8101 tgcctgcggc gcgatctcgt gggcggttcg gaaatggact ttggccactt cacgcaagat
     8161 gtgcggccct tgcctttcga ccagctgctt cgccgtctct ttcacattcg aagaaacgcc
     8221 gcgtcccaag cgcacgccca gcgtcttccc tcgccgattg agccaatcga tgaatgcttc
     8281 gcgcgccaga atcgaggccg ccgcaaccgc aatgtcggac tcggcgcgcg gccgctgttc
     8341 gatttcgatt ttgcgtccgt ggcgtagcaa tgcgttttca atcacgcgcg catctgcaaa
     8401 ctggtcggac aacgaacgtg gacactccgg ttttttcgcg agcagatttt caattacccg
     8461 cgcgtgaccc caccctagca atgcgttcag gttggcaaat ttcgcgtaga gctcgttgta
     8521 cttcgcgggc ccgatcagca ccgtttcatg cgaaccggcg gtgctcttgc gaattgtttt
     8581 cgcaagatcg cgaatgcgcg catccgaacc gatgcgtttg ctgtcgacta ctccggcatc
     8641 gcgcagcttt cgcgcaattc caagatcgac atacaccccg gcgatcacga gcggaccgaa
     8701 gaagtcgccc ttgccactct catccacgcc aaaatgcggc tggaacattt ccggtgaatg
     8761 cacttcctcg taacccaatc tggcttcgcc gagaattttc ggctccagtt cgaactgcac
     8821 gaactcttcc gctcctttgc cctgcacgag caccttcggt cctttctcgt acaccgcgac
     8881 gctcaatttg ttcttctgcg caaaaaaaac tgtgtactcc ttcggtgaaa actcgaagcc
     8941 gagctcgtgc agcaatgcac gcagcttgtc cacctgctga gtcgtcacca gagcggtaaa
     9001 ggaattcatt ccgccacaga ttaacacaga tgaaacacag atcgcctcgc acgaaaccta
     9061 atctgtgtta atccgtgttc atctgtggct attcgagcag tgcagttccg tcatgcgctt
     9121 ctgcgctggt atcgaaaaca cggccgcgat ctcccctggc gacaaacccg caatccttac
     9181 gccgtgctcg tctccgagtt catgctccag caaacccagg tggccgccgt actacctttt
     9241 tataatgagt ggttgcggcg ctttcccgat ttcgccgcac ttgcgcgtgc atcccaaaag
     9301 gacgtcctgc acgcatggca ggggctcggc tactatgccc gcgcacgaca tctccacgcg
     9361 acagccaagc tcgtgcaaga tcgacatcgg gacggttttc cgtccgaaat cgacagcatg
     9421 cgggaactac ccggcgtcgg tcgctacatt gcacacgcga ccgcgacctt cgcattcgac
     9481 caagccgtcc ccgtagtcga agcaaatacc agccgcgttc tcacgcgtct gttcgatcta
     9541 cgcttaccca tcgaccatac agctggtcgc aacgcgcttt ggcagtacgc ccgcgccctg
     9601 ctgccgccaa ccggtgcacg catttacaat tccgcgctca tggacctcgg cgcgcttgtt
     9661 tgcctgcgcc gcccaaaatg cgccatttgc gccgtgaaaa cattttgccg cgcaaacaga
     9721 ccggaaactc tcccagtcaa acgcgcgcgt cctcccatcg cgtatcttgt ggagaaccac
     9781 gcgctcgtgt cgcggcgaaa taaaattctg ctgcaagaag cgcagcatcg ctggcgcggc
     9841 atgtggattc tgccgcgact ggcacttgac ggctttaagc cgccaagttt gcagccgaat
     9901 gaagtttaca ccggtgtgtt tccattcacg catcaccgga tcactttgaa ggtcttcgtg
     9961 cagcgcaagc gtaagatcga cagttatctt gggcgttggt tcaatttgaa cgcgcttgat
    10021 acaattccga tcccgacacc gcatcgtcgc gccatcatat cgctcctcaa accggaaggt
    10081 agccacacgt tgaggttgaa ggaacattcg cctgcaatta cagaagactc agatgagaag
    10141 catgaccggt tacggtcgcg gcgaggcaga gcacaacggc catcggttct gtgttgaact
    10201 caactcggta aaccgcaaac agagcgacgt agtcgtaaat ctgccgcgcg atctgaccgc
    10261 gctcgagccc cgcattcgcg agacaattaa cgagaacatc tcgcgcgggc ggacgaacgt
    10321 caccgtcaca ttgcgcaatc gcgccaatgg cgcacagcaa ctcgcactcg atacgaatct
    10381 ggcccggttc taccaccaag cgatgcgcgc cttgcagcag gagctgaacg cgccgggcga
    10441 gatcacgatc ggcctcattt tacaggcgcc cggcgtgatg cgactgcccg agcaagtcgt
    10501 cgatcccgac gaagtctggc cgcgcgtcga acaagcactc cgcgccgcgc tcatcaatct
    10561 aatcaaaatg cgcgaacgcg aaggcaaaca cctggccaag gatttgattc atcggctgaa
    10621 gcagattcgc aaaaagctga aggaaatccg cggcctgcat cccgacgtaa cgaagaagta
    10681 tcgaacggcg ttgctggatc ggctggagaa agccggactg ccgctcgacc cggccgacga
    10741 gcgaatcgtc aaagaaattt cgtttttcgc cgatcgcacc gatgtgtcgg aggaattaac
    10801 gcggttggaa agccatctcg cgcagttcgc gcatcacctc cggcgaaacg agccggtcgg
    10861 gcgcacgctt gagttcatcg cgcaggaaat ttcgcgcgag ctcaacaccc ttggcgcaaa
    10921 aggaaacgat gccgcgattt cccagcgcgt tgtcgcctgc aaagcggagc tggagaaaat
    10981 ccgcgagcaa gtgcagaacc ttgagtaagc tggaaagtgg catcggcatc ctgccgatga
    11041 ggacatgggc tgcaagccca tgccactact gtcgcaatca gttcttatta aagcgatgag
    11101 ttccagcgaa ttcagtcgct acggaattct cttcgtcatc tccgcgccct ccggtgcggg
    11161 caagacaaca ttggtcgaag cgctccgaca aacggcgaat ttgttctatt ccgtttcctg
    11221 tacgacgcgc gcaccacgcg gcggggaaat ggatggtgaa gattatcggt tcctctcgga
    11281 cgccgagttt caagcgcgcg tgaaagccga cgaatttctc gagcacgcgc aagtgcatgg
    11341 cgaccactac ggcacgttgc gcgacccggt cgtcgcaaat ctaaaaaagg gcgtcgacgt
    11401 tttgatcgac atcgatactc agggcgcggc caccatccgc agttgcgatg acccgttcat
    11461 ccgcgcagcg ttgacagaca tcttcattat gccgcccgat ctcgacgagt tacgccggcg
    11521 tctcctgaaa cgcggcaccg agacgaccga aaaaatcgag ttacggctgg ccaatgccgc
    11581 acgggaaatg caattttggc gcgactatcg ctacactgtc atcagcaaat cgatggaaga
    11641 agacctgcaa aagttgcgtc acatcatcgc ggcggaaagc tttttgagcc ggcggctcat
    11701 ccgcaaatga gcagccaatt gattgtcgat cttgcatgaa gccagattcg aagagccgag
    11761 ctgataaaat cagtgcccgt aaagtcgtgc gcctgcgcgc cggcaagaga acagtcgtgc
    11821 tcggtgtcac cggctcgatt gccgcttaca aatccgccga gctggcgagt ctgctcgtga
    11881 agcagggcca cgatgttttt gtcgtgatga cgcaccatgc gaccgagttc atcgcgccgc
    11941 tcactttgca aacactttcc aaaaacccgg tgatgaccag tttcttcgac gaaaaagaaa
    12001 actggcgccc agggcacatc gaattggccg atcgcgccaa cctgttgctc atcgcgccgg
    12061 caaccgcgca cattatcgcc gagctcgcgc acggcctcgc caatcatccg ctcgccgcga
    12121 ttgcgctcgc cacccgcgcg ccgattctgc tcgcaccagc aatgaatgga aaaatgtggg
    12181 accatcccgc gactcaagaa aatgtcgcca aattgaaatc gcgcggcgtc gaattgatcg
    12241 gaccagaagc aggcatgctc gcctgcggtt acgaaggact cggccgtttg tggaaggtcg
    12301 acgacattgc tttccgcgcg gaattcatgc tggcgcggca ggaaaatctc atcgcgtgag
    12361 gtttgttgtc acggccgggc caacgcgcga gccgatcgat cccgttcgct atatcagcaa
    12421 tcgctcgtct ggaaaaatgg gctacgcgat tgtcgaagcc gcaatcacga ccgggcacga
    12481 cgtcacgctc atatccggcc ccgtgcactt cgatcagcca cacggcgcac atgtcgtttc
    12541 agtcatgaca agcgacgaga tgttttacgc ggtacatcaa catctccgcg actgcgacat
    12601 cctggtcatg tgcgccgcgg tcgccgatta caagccggcg atcgtcgccc cgcgcaaaat
    12661 caaaaagcgt gatgccagtc tcacgctcga gttggtcccg acccgcgaca tcctcacctc
    12721 gatcccgcac gatcgagaat ttcttgtcat cggcttcgcg gcggaaacag acgagctcga
    12781 aaaaaaagcc gcgcaaaaat tgcgcgccaa aaattgcgac atcattgtgg ccaacgatgt
    12841 aagccgcgct gacgccggaa ttgagagcga tgaaaatgcg gtcacgcttt tgtttcgcgg
    12901 cggtaaaaca aaaaaaattt cgcgggcgcc gaaaaagatc atcgcgcgtg agctggtgga
    12961 aattttttta aatgcgcgcg aaaaacggtt gacaacaaaa acgtgatgat tacttgactc
    13021 gttgaagtga agtcattccc aactcttcac gcaataccgc cgacgcggat tgaaaggggg
    13081 gaatagtcga tgccaaggaa aaaagcagca aagaaaaaat cagcgaagaa aaagaggcac
    13141 taaggcccaa cgcttgatca cgattccgaa tgctttcgga atcacaaccc aacttcaacc
    13201 cgggaggaga actcgttttc ctcccggttt ttatttcgcg cggcgttggc ggagcggctg
    13261 atctgctgtt gtaggacgag cgctgctaaa tcagttggca gggcaacggc gcaattctat
    13321 ctgtgtgaaa tgaagcatta tctcgaggcg gcggagaacg ctgcgcgcgc cgccggagat
    13381 ttgctgcgcg aaaattttca accccgaaag cattcggggc tgcgcgtcaa ctccgccgag
    13441 gcgcatgaca tcaagctcga gattgatgtg cgcgcgcagg aactcatcac ctcctcgctc
    13501 ctgaaaaact ttccgcaaca tgcgctttac ggtgaggaag gtgtcgtcgg cgaccagacg
    13561 agcgagcacc agtgggtcgt cgacccgctt gatgggaccg tcaattttta ttatcgcatt
    13621 ccgcacttct gcgtttcgat cgcgcttcga ttcaagggtg aaatcatcgt cggtgtcatt
    13681 tacgatccaa tccgcgacga gatgtggagt gggcaaaagg gagaaaaacc gcgcctcaat
    13741 gggcaacatt ttcgcgtcag tgagcgaacc gagcttgccg aagccgtcat ttccgtcggc
    13801 ctctcaaaga cgggcgtgct catcgagtcg aacgtcccga tgttgcatca gatgattcat
    13861 cgcgcgcgca aatgccgttt gttaggcagc gccgcgctcg acatggctta cgtggcctgc
    13921 ggtcgtctcg atgcctacat cgaacaaggc atcaacttat gggacatcgc agcgggctgg
    13981 attttagtcg aaacggctgg cggctcagtc gatctgcgcc cgcgtaaaga tatgaaggac
    14041 aaatacagca tcatcgcctc gaacggcgtg atcgatttaa aattgtgagc accacgcgct
    14101 gcggcatcgg ctatgacatc catcgtttcg ccgaagatcg acaactgatt ctcggcggag
    14161 ttgaaatcgc gaacacgcgc ggcctggccg gccactccga tgccgatgtg ctctcgcatg
    14221 cgatcgccga cgcgctgctc ggtgcgatcg gcgcaggcga catcggtcaa cattttccaa
    14281 ataccgacga ggcgcttcgc gggatcagca gcatcgaaat cctgcgccgc atcagcgcac
    14341 tgctcgccga gaaaaaggcg cgagtcgtaa acatcgatgc gacgatcatc gccgaagcgc
    14401 ccaaaatcgc gcctcatgtt tctgcaatgc ggaaagacat cgcggcggcg attggggtca
    14461 acggagcgaa catcagtatc aaagcgacga ccaatgaaat gctcggcgca atcggccgcg
    14521 gagaaggcat cgccgcaatg gcaatcgcca ctgtggaaca agaatgagtc cattgtcatt
    14581 ccgagcgaac catttgtcat gtcgagcgaa gtcgagacat ctcttattgt taaccgatga
    14641 acagtgagag atttctcgac tgcgctgtgc tccgctcgaa atgacaaaat agcaatggcg
    14701 cttcgtttct ttaacactta ctcgcgccag ctcgaagagt tcaccccgcg cgatccgagc
    14761 tcacactcgc cacccgacga ggcccttcga ccggcggacg cgatcgacat gtacacctgc
    14821 ggcccgacgg tttatagccg cgcgcacatc ggaaattttc gcgcatacgt ttttgaagat
    14881 ttgcttcagc ggcatctcga atcccgcggt tatcaggttc accgcgtcat gaatatcacc
    14941 gacgtggatg acaaaactat tcgcggcgcc cgcgaagccg gcgtgccgct gcagaagttc
    15001 acggcgcaat tcaaggacgc ttttttccaa gacatcgaca cgctgcgcat caaacgcgcg
    15061 gaccaattcc cggccgcaac cgaccagcgt tacatcgaca agatgatcgc aatgatcaag
    15121 acgctgatcg accgcggctt ggcctacctg gcggacgaca aatccgttta ctatcggatc
    15181 aacaaatttc cgaattacgg caaactcgcg catttcgatc tgacgcagtt gcaatcgacc
    15241 ggacgcgtga agcacgatga gtacgacaag gaacacattg gcgacttcgc tctatggaaa
    15301 gcttgggatg aagaagacgg cgaggtgaaa tgggactcgc cgtggggccc gggccggcct
    15361 ggttggcata tcgagtgcag cgcgatggcg atggcgttgc tcggcgacca gatcgacatc
    15421 cactgcggcg gtattgacaa catttttcca catcacgagg cagagatcgc gcagagcgaa
    15481 ggcgtcaccg gcagacagtt cgtccgctat tggctgcact gcgcgcacct gttggtcgat
    15541 gggcagaaga tgtcaaagtc gttaggcaac ttctacacgt tgccggacgt gctggcaaaa
    15601 ggttacaccg gacgcgaggt gcgttacgcg ctcatgcgcg tgcattaccg cgcgccactc
    15661 aattttacgt gggaaggaat gaaagaggcg cgcgagtcgc taggccgaat cgacgattgg
    15721 ctgcaacgct tgcgcgaatg tagcgcaagc gtctcgcttg cgaaaaccga caaagggcaa
    15781 gctggaagct tgccctgcac ttttgaagac gcgctcgatg acgatctcaa catctccggc
    15841 gcgcttggtt ttttgttcga aagcattcgc gagacaaatc gcgcgctgga tcacaaagag
    15901 ctcgactcca gcgccgcgga ggagtggttg tcgtggtggc agcgcattaa ccgcgttctc
    15961 aacgtcgaaa agcacgatgg cgcagctccg ccggaaatcg cgaaactggt ggaggcgcgg
    16021 gcgcaggcga ggcttgcaaa agatttccgg aaaagtgatg aattgcgcga cgaattgagc
    16081 gcgcgcggct gggaagtacg cgacacgaag gacggaccaa agctgacacg tcgcggcggc
    16141 gcttgatgcg ttgaacatgt ttgcgccggc gcaattcttt agcctcgacc acacggcgca
    16201 cccgaagttg ttcgagaacc agaattacgt ctgggatgcg ctcaagcaga tcgcgagtta
    16261 tctgcagttc cgtttaaaac cggcgatcct tggggacttg gtggggaagc cgttcatcag
    16321 taaccacgtt ttcatcggcc gcggcacgat tgtcgaacaa ggcgcggtgt tgaaaggccc
    16381 ggc
//
LOCUS       DASTDG010000210        15165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1006579, whole genome shotgun sequence.
ACCESSION   DASTDG010000210 DASTDG010000000
VERSION     DASTDG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006579"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="msrA"
                     /locus_tag="VFO30_06935"
     CDS             <1..273
                     /gene="msrA"
                     /locus_tag="VFO30_06935"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9419060.1"
                     /translation="EQLLDVFWSSHNPTTLNRQGPDVGTQYRSVIFYHSPAQKASAEA
                     SKQSIAKSGRFNRPVVTQIEPAPKFWPAEEYHQRYLQKRGQSHCAV"
     gene            286..984
                     /gene="pgl"
                     /locus_tag="VFO30_06940"
     CDS             286..984
                     /gene="pgl"
                     /locus_tag="VFO30_06940"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9419061.1"
                     /translation="MSREVIRTKNFAAEAPNFILSVAHKALGERGEFRIALSGGNTPR
                     PVYTQLAAVAHDLPWERVIVTFSDERCVPPDDAQSNFRMARESLIAPAAIPDKSVLRM
                     RGEIEPHLAAQEYQDELDVLATQRGEHVYQHDLILLGLGDDGHTASLFPDTTALKETN
                     RRVLANFVPKLNSWRLTFTFPLIDHGRHVCFLVSASKGQLIDRVLNGDRNYPAARVNP
                     TAGELTWIIDLSGS"
     gene            complement(1008..1418)
                     /locus_tag="VFO30_06945"
     CDS             complement(1008..1418)
                     /locus_tag="VFO30_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419062.1"
                     /translation="MEYTDAPIAETAAQDADKMPCPYCGHLLPKHAQRCDRCDWTRSG
                     TETAEGKASDAMAILLSIIPGLGHIYKGHKLVGSLILFVGTPMAIAIAALTFTFTAGF
                     GGLLLPLWWFVVMFHVYGIEDRIGPPTDDEGEQY"
     gene            1509..2264
                     /locus_tag="VFO30_06950"
     CDS             1509..2264
                     /locus_tag="VFO30_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9419063.1"
                     /translation="MALLREIVRYMDKYLRIHEIGDWDNALNGLQIENSGRVSKIGAA
                     VDVSTRALAEAAKQHVDLLIVHHGLFWPGLQRVSGWLHRQLKLAFTNDIALYSAHLPL
                     DLHPQIGNNAQLVKALGVKSTTPFFEEKGQLIGLKARTSISRDEILLRLEKSLGQKAK
                     TFSFGPPEPKTVGIVSGDAGGEIYRVAAEKIDTFITGEAPHWAAIAAEELRLNLILGG
                     HYATETFGVKALAAHLSRRFKIPWTFLNLPTGL"
     gene            2291..3883
                     /gene="murJ"
                     /locus_tag="VFO30_06955"
     CDS             2291..3883
                     /gene="murJ"
                     /locus_tag="VFO30_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET9419064.1"
                     /translation="MMPQDRHISTRATGVVGIAVMCSRVLGLIREQVFAGLFGAGKNL
                     DAFLMAFRVPNLLRDLFAEGALSTAFITTFSKKIATEGDTSAWRLANKVATLSAVFMS
                     AVTLLGIVFAPQLIHLMTWWSWSPEKTAMTILLTRVMWPFILLVSLAALVMGILNAKH
                     VFGAPAMASSYFNLGSIIGGVAFGWWLDPHFGARSLIGLAIGTLIGGLWQLSAQFPSL
                     RRVGYRYSADFHWRDQGVRTVLALMGPAVIAASAVQVNVLVNSGFAARLGDGPVSWLN
                     IAFRLMQLPLGIFGVAVGTVTLPLVSRTAALGDTSAFRSALAHAIRLVILLTVPAAIG
                     LIVLARPIISVIYEHGRFTPEATLQTAAALQFYAIGLVAYSAVKVLAPAFYAIDKRYL
                     PMVVSMLSILVNFALNWFFMLKLELGHRGLALSTSLVALTNFFLLYGMMRRHAGRLET
                     TELFKTVGKVAVAGAVLAVVCWLALEFLFSHTPLPLWKKVVDLAITIVLGVFTFFGTA
                     FLLRVTEVRDVIDLLWRRFGRR"
     gene            complement(3932..5050)
                     /locus_tag="VFO30_06960"
     CDS             complement(3932..5050)
                     /locus_tag="VFO30_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET9419065.1"
                     /translation="MRIETLAVHAGHSIDPATGAVATPIHLSTTFERDIEGTYSRGFM
                     YTRNNNPNRQALENGIAALEGGAAAAAFASGTGATMSIFHALAPGDHVLAHIDAYYGT
                     SRLLREIFLPWGLAADFIDMSDVAAVKKALRPNTKLAWAETPSNPLLKVVDLAAVAEI
                     VRDTGAICVCDNTWAPVLQRPFELGADLIVHSTTKYFGGHCDVAGGIVVSKIDDEFFQ
                     RIRAIQYCGGAVPSPFDCWLILRGMRTLPWRMRAHSDNALKVAEFLAQHRQVERVHYP
                     GLTTHPGHAIARKQMSIFGGMLSLEVKGGRDAAIAVTAKTKIFIRATSLGGVESLIEH
                     RASIEGPGTTSPENLLRLSIGLENADDLIEDLDQALTL"
     gene            5295..6560
                     /locus_tag="VFO30_06965"
     CDS             5295..6560
                     /locus_tag="VFO30_06965"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9419066.1"
                     /translation="MSTIYDDEVFKMACDQFQVIADYLSIDKNDRERLMLPKRAIAVT
                     LPVHMDDGSTRTFQGYRVQHHLTLGPTKGGTRFAPSLSMGETAALAMWMSWKCALSNL
                     PYGGAKGGVACDPTKLSRTELEGVSRRYMQEMIPFVGPKTDIMGPDMGTNEQIMAWFM
                     DTYSVYQGYAVNEIVTGKPVAIGGTEGRREATGRGVVYLIERAMNILKFDPEKCTAIV
                     QGFGNVGSVAALSLAYKTGTKVVGLSDAYAAIYNPKGIDVQAAEQHVLKKGNLRAFDE
                     ANHVDPKELLTMPCDVLVPAAVDRVINGANAGRLQCRILAEAANGPTTPEADLILEKR
                     WDEIFVIPDILCNAGGVIVSYFEWVQGLQSFRWSESEVTDKLFRILEHSFAQVIRRAK
                     SHKISHRTAAMAIGVERVLRAKKMRGLFP"
     gene            6567..7502
                     /locus_tag="VFO30_06970"
     CDS             6567..7502
                     /locus_tag="VFO30_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419067.1"
                     /translation="MSFSHREETRQGRQLDVLTDEENGIRVVVSRLGAELVSLARINE
                     TGDWVGFLYRDNDISAPAHGWANHATVMGYYLHRLKDDRSFYRGHEIKGGTHGFLRHK
                     TWHFAGAENDSLTYRITPDDFPATDYPLKVSLDLTYKIETNKITVTFAFRNDEPELTA
                     HVGFGLHPGFAATSFESFRLQMPRGLYRRHFSPGNYLSGETRDLEFRGGEMPFPREEL
                     PGSFILELVEVPRREFSYVDPPSGRWVIVDLAEVPYLTLWSDGGPFLCLEPCWGLTDH
                     HQQRALEDKEGMQRIAPGAPLRASFSMAPQFASSD"
     gene            7536..8423
                     /locus_tag="VFO30_06975"
     CDS             7536..8423
                     /locus_tag="VFO30_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419068.1"
                     /translation="MPAASLYALLAHSIDYAGMFPPCSLDLESASHNQAQYVRSPDAW
                     MLNSFVLPIDQFEAAAPNLSLFDKKHALRVSALGAKDADLKNALEAIRDFARQHVDVV
                     SVVQLEMPLATDVDLAWLREAGATVGDLKLHVFWEAPPDAAEETIGLLAQLKEPRFGY
                     KLRTGGVSADMFPSAVQIAEALVAATKHHVPIKFTAGLHHPIRQFRDEVGTQMHGFLN
                     VLGAGVLASEHHWDEGQVVQMLEDERPASFSFDETIFAWREWEITAERIHARRKYVTS
                     FGSCSFDDPRDDLRAVGLL"
     gene            8514..9776
                     /gene="fahA"
                     /locus_tag="VFO30_06980"
     CDS             8514..9776
                     /gene="fahA"
                     /locus_tag="VFO30_06980"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HET9419069.1"
                     /translation="MPLRSFIDIAPDSHFPLENLPFGVFKPRDSAVRIGVALGEYVVD
                     LAELERAGAFKDLPPETAAATTHRTLNRLLPLGRPEWRRLRHKLQHLLSADTATLRDD
                     TKLRERVLHRQRDVTMQLPARIGNYTDFYSSYHHAHNLGTMLRGPENALMPNWKWLPV
                     AYHGRTSSVVISGTEVRRPQGQVKPPDAPAPIFGPTRSLDYELEMAFLIGPGNSLGRP
                     VPIERAVDHIFGFVLMNDWSARDIQTWEYQPLGPFLAKNFCTSISPWVVTLEALEPFR
                     KPLPKQDPEPLQYLRLTNDFTYDIHLEAALQTAAKDRHVITRTNFQNLYWSISQQLAH
                     HTVGGCNLEPGDLLASGTISGATEESRGCMLELTWRGAKPLKLPNGETRKWLEDGDTL
                     AITGWCDGGDYRVGFGEVTGRVVPAAAA"
     gene            9840..10910
                     /locus_tag="VFO30_06985"
     CDS             9840..10910
                     /locus_tag="VFO30_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419070.1"
                     /translation="MFKTKFAPLRFFLFTFALVLPVASWAANPYQSRNMHFGVSGSNV
                     KDITRAYCCGGTLGSLLSDGTTQYILSNNHILARQDRATPGENISQPGLIDNGCQPAT
                     IVADFTAAPKLGTNVDAAVAALRAGKMDSTGYIEGIGTISSVTKAPAVGLAVEKSGRT
                     TGTTTGTINSVNTSVNVQYQIRCGQGKKYVISYTNQIVINGSGFSAGGDSGSLIVSNT
                     SNCHQPVGLLYAGSSTDTIANPIGEVLNKVGTALGRSVNFVGSTCTSSPAQSAQATGA
                     EGLQLPEQAVEHTSRVLEQHRHDLMSRPGVLGAGVGASESGNEAAVVVYVDNTAGAQP
                     QLPAHVDGVPVKVHLTDPFVAF"
     gene            11095..11859
                     /locus_tag="VFO30_06990"
     CDS             11095..11859
                     /locus_tag="VFO30_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9419071.1"
                     /translation="MKAASIEAAGDRSLNAARLLNDHMDERLVRTRRVFLKSGILIGG
                     AALVGMHGMRGAEKEEEQVSPSEDLMREHGVLKRVLLIYDEAIRRMTANQEFPVETVV
                     DSAKIIRSFIEDYHEKLEENFLFPRFEKAGQLTDLVKTLRDQHEAGRGVTDLTLNLAT
                     QKTLKNAAERPRLGNALRQFVRMYNPHEAREDTVLFPAFRKIVSANEYASLGEDFEKK
                     EHELFGEEGFEKMVDKVTSIEKKLGIYELAQFTPKI"
     gene            11906..13042
                     /locus_tag="VFO30_06995"
     CDS             11906..13042
                     /locus_tag="VFO30_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9419072.1"
                     /translation="MGVANFFADLTYEGARGITGPFLASLGASATVVGFVAGFGELVG
                     YSLRSVTGYFADRTRKYWLVTFVGYVINMLAVPALALAGNWPVAAGFIVAERTGRAIR
                     KPSVEAMLSDAGKSIGSGWAFGLNEALDQAGAMLGPLLIALVLFRGRNYQSAFALLLI
                     SAILCLGIVAVARLSHGRSQSVERAASPTLAMPLSLMPYWIYVAAGALIAAGFADFSL
                     IGFHFRKASVVSDGVIPIYYAAAMGTGAVGSLLFGRLLDKLGQKVIVFVFLVSALFAP
                     CVFLGNSVVAFAGMALWGLSMGAQDSLLKAVLTPVVPAGKRSRAFGLFDTVYGVAWFL
                     GSALMGFLYDKSIVALVLFSVVLQVAALPLLICANLHPSNRGQS"
     gene            13092..13496
                     /locus_tag="VFO30_07000"
     CDS             13092..13496
                     /locus_tag="VFO30_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9419073.1"
                     /translation="MQGDTVESKTDKLRILHVDDDPDIRLLIAGSLQDFGYVVVTAGT
                     VAEALQLAREMHFNLCILDVRLPDGTGIELCQQLRQLQPNTPVLYYSAYASDSEQKEA
                     LSVCGDAYLKKPVLAEELEQTITRLLNREQQD"
     gene            13753..>15165
                     /locus_tag="VFO30_07005"
     CDS             13753..>15165
                     /locus_tag="VFO30_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9419074.1"
                     /translation="MKKTSSAPVTRPGNGARFNGSEVDAKQLLTALIAFKRGDFSARL
                     PDDWTGVAGKIADTFNDVIATNQRMTRELERIGRVVGKQGRITQRASLGDVSDSWADA
                     IGSVNDLIGDLVQPTSEMARVIGAVAKGDLSQTMATEIEGRPLEGEFLRTAKVVNTMV
                     DQLGAFASEVTRVAREVGTEGKLGGQAKVKGVAGTWKDLTENVNLMAGNLTAQVRNIA
                     TVTTAVANGDLTKKITVDVKGEFLELKDTVNVMVDQLRSFASEVTRVAREVGSEGILG
                     GQARVEGVSGTWKDLTDSVNFMARNLTGQVRNIAAVTTAVARGDLSKKITVDVKGEIL
                     ELKNTINTMVDQLSSFASEVTRVAREVGTEGKLGGQADVKGVAGTWKDLTDSVNSMAG
                     NLTAQVRNIADVTTAVATGDLSKKITVDVRGEILELKDTINTMVDQLRSFASEVTRVA
                     REVGREGKLGGQADVRGVAGT"
BASE COUNT         3164 a         4275 c         4484 g         3242 t
ORIGIN      
        1 gagcaactac tcgacgtttt ctggtcgagt cacaacccga caactttaaa ccgccagggc
       61 cctgatgtcg gaacgcaata tcgttccgtg attttttacc acagcccggc gcaaaaagcg
      121 tccgccgaag cttcgaaaca aagcatcgcc aagagcggcc gcttcaatcg gccggtggtg
      181 acacaaatcg agccggcgcc gaaattctgg cccgccgaag agtaccatca acgttatctg
      241 cagaagcgcg gccagtcgca ttgcgcggtt taggcgaagc cgcgcatgag ccgcgaagtc
      301 atccgcacaa aaaatttcgc agcggaggcg cccaatttca tcctcagcgt agcgcataag
      361 gcgctcggcg agcgcggcga attccggatc gcgctttccg gaggtaacac gccgcgaccg
      421 gtctacacgc agctcgctgc cgtcgcgcac gatctcccgt gggagcgagt catcgtgact
      481 tttagcgacg agcgctgcgt gccgccggac gacgcgcaga gcaatttccg aatggcgcgc
      541 gagagtctca tcgcgcccgc ggccattccc gacaaatcgg tcttgcgaat gcgcggcgaa
      601 atcgaaccgc acctcgccgc gcaggaatac caggacgagc tcgatgtgct cgccacccaa
      661 cgcggcgaac acgtctacca gcatgacctc attttgttag gcttggggga tgacggccac
      721 acggcgtcgt tgtttccaga caccacggct ttgaaagaga caaaccgccg cgtgttagcg
      781 aattttgttc cgaagctgaa ttcgtggcga ttgactttta ccttcccgct cattgaccac
      841 ggtcggcatg tctgtttttt ggtcagcgcg agcaaaggcc aattgatcga tcgtgtgtta
      901 aacggcgacc gcaactatcc ggcggcgcgc gttaatccga ccgccggcga gctgacctgg
      961 attatcgatc tgagcggctc gtaaacctcg ccagcgcgat tcggtatcta atactgctcg
     1021 ccttcatcat cggttggcgg cccaatccgg tcttcgattc cgtaaacgtg aaacatgacc
     1081 acgaaccacc agagcggcag cagtaacccg ccgaacccgg ccgtgaatgt gaacgtgagc
     1141 gcggcgattg cgattgccat cggcgttccg acaaacagga tcagcgatcc gacgagctta
     1201 tggcctttgt agatgtgacc gagaccgggg atgatactta ataatatcgc catcgcatcg
     1261 ctcgctttgc cttcggccgt ttccgtcccg ctgcgcgtcc aatcgcagcg gtcgcacctc
     1321 tgcgcatgct ttggtagcag atgaccgcag tacggacacg gcattttgtc ggcgtcctgc
     1381 gcagcggttt ctgcgatggg cgcatcggtg tattccattc ctaaagagcg tcacaacgcc
     1441 cggcgctttc gtcaagaaaa catgcctcct tgtcattgcg agcgcagtcg aggaatctct
     1501 caatctgcat ggctctcctt cgcgagatcg tccgctacat ggacaagtat cttcgcattc
     1561 acgagatcgg cgattgggac aacgcactga atggcttgca aatcgaaaac tccgggcgcg
     1621 tctcgaaaat tggcgcggca gtcgatgtct cgacccgcgc gctggccgaa gctgcaaagc
     1681 aacatgtcga tcttttgatc gtgcatcacg gcctcttctg gcccggtttg caacgggtga
     1741 gcggttggtt gcaccgtcag ctcaagctcg cgtttacgaa cgatattgcg ctttacagtg
     1801 ctcatctccc gctggacctg cacccgcaga tcggcaacaa cgcccagctc gtcaaagcgc
     1861 tcggcgtcaa atcgacaacc ccattcttcg aggaaaaggg gcagctcatc ggattgaaag
     1921 cgcgaacgtc gatctcgcga gatgagatct tgctccggct cgagaagtcg ttaggccaaa
     1981 aagcgaaaac cttcagcttc ggcccgccgg aaccgaaaac agttggaatc gtgagcggcg
     2041 acgcgggcgg ggaaatttac cgagtcgcgg cggaaaagat cgacactttc atcaccggcg
     2101 aagcgccgca ctgggcggcc attgccgctg aagaactgcg actcaacctg atcctcggcg
     2161 gccactacgc gaccgagacc ttcggcgtca aagccctcgc cgcgcatttg tcgagacgat
     2221 tcaaaattcc gtggacgttc ctgaatttgc cgacagggct gtgactgctg ctccaaagca
     2281 aattgagcct atgatgccgc aagatcgaca tatcagcacg cgcgccaccg gcgtggtcgg
     2341 catcgcggtg atgtgctcac gtgtgcttgg gcttattcgc gagcaagtgt tcgccggtct
     2401 ctttggcgcg ggaaaaaatc tcgatgcctt tttgatggcg tttcgcgtcc ctaatctttt
     2461 gcgtgacctg ttcgcggaag gcgcgctctc gaccgcgttt atcaccacgt tttcgaaaaa
     2521 gattgcgacc gaaggcgata cgtcggcgtg gcgattggcg aacaaggtcg ccacgttgag
     2581 cgcggtattc atgagcgccg tcacgctgct cggcatcgtc tttgcgccgc aactgattca
     2641 tctcatgacg tggtggagtt ggtcgcccga aaaaacggca atgacgatcc tgctcacgcg
     2701 cgtcatgtgg ccgttcattt tgcttgtctc gctcgctgcg ctcgtcatgg gaattttgaa
     2761 cgcgaaacat gtcttcggcg cgcccgcgat ggcgtcgagc tatttcaacc tcggttcgat
     2821 catcggcggg gtcgcattcg gctggtggct cgatccgcat ttcggcgcgc gttcgctaat
     2881 cggcctggcg atcggcacat taatcggtgg attgtggcaa ttgagcgcac aatttccctc
     2941 gctgcggcgc gtgggctacc gctacagcgc cgattttcac tggcgggatc agggcgtgcg
     3001 caccgtgctg gcgttgatgg gtccggccgt catcgcggcg agtgcggtgc aggtcaacgt
     3061 gctcgtgaac agcgggttcg ccgcgcggct cggcgacggc ccggtcagtt ggctgaacat
     3121 cgcgtttcgc ctaatgcaat taccgctcgg catcttcggc gtcgcggttg gaactgtaac
     3181 cttgcccctc gtgtcgcgca cggcggcgct tggcgatacg tcggcttttc gttccgcact
     3241 cgctcacgcg atccgactgg tgatcttgct gacggttccc gccgcaatcg gcctgatcgt
     3301 gctcgcgcgg ccaattatca gcgtcattta tgagcacggc cgctttaccc ccgaggcgac
     3361 tctgcagact gcagctgcac tgcaatttta cgccatcggg ctggtcgcgt attccgccgt
     3421 caaagttttg gcgcccgcgt tttacgcgat tgacaagcgt tacctgccga tggtcgtcag
     3481 tatgttgtcc atcctagtga acttcgcgct gaactggttc ttcatgctca aactcgagct
     3541 cgggcaccgc ggtcttgcgc tctcaaccag tctcgtagcg ctgacgaact tcttcctgct
     3601 ctacggcatg atgcgtcgcc acgccggccg actcgaaacg accgagctgt tcaaaaccgt
     3661 tggcaaagtg gcagtcgccg gtgcggtgct cgccgtagtc tgctggttgg ctttggagtt
     3721 tctgttttcg cacacgccgc tgccgctgtg gaagaaagtt gtcgatcttg caattacgat
     3781 cgtgctcggg gtcttcacgt tttttggcac agcatttttg ctgcgggtga ctgaggtgcg
     3841 cgatgttatc gatcttctct ggcggcgatt cggccgccgg tgattgtaga ggcgtttgtg
     3901 tcaaacgcct cggcgcttgg cacaagcgcc gctacaacgt cagcgcttga tcgagatcct
     3961 cgatcagatc gtcggcattt tctaaaccaa tcgataaacg cagcaagttt tcgggtgaag
     4021 ttgtgcccgg cccttcgatg gacgcgcggt gttcgatcaa gctttccacg ccgcccagac
     4081 tggttgcgcg aataaagatt ttggtctttg ccgtcaccgc aatcgctgcg tcgcgaccgc
     4141 cttttacttc aagcgaaagc attccgccga agatcgacat ctgcttgcgc gcgatcgcgt
     4201 gcccgggatg agtcgttagg ccggggtaat gcacgcgttc gacttgtcga tgttgcgcga
     4261 gaaactcggc gaccttcagc gcgttgtcgg aatgcgcgcg catgcgccag ggcagcgtgc
     4321 gcatcccgcg caaaatcagc caacaatcga acggcgaggg gacggcgccg ccgcaatact
     4381 ggatggcgcg aatcctctga aaaaattcgt cgtcgatctt actcacgaca attccgccag
     4441 cgacatcgca gtgaccgccg aagtacttcg tcgtggaatg cacaatcaaa tcagctccca
     4501 gttcaaacgg tcgctgcaat actggcgccc aggtattgtc gcagacacaa atcgcgccgg
     4561 tgtcacggac gatttccgca acggcggcaa gatcgacaac cttcagaaga ggattgctcg
     4621 gcgtttccgc ccaagccagc ttcgtgtttg gccgcagcgc tttttttacg gcggcgacgt
     4681 cgctcatgtc gatgaaatcg gctgcgagcc cccacgggag gaaaatttcg cgaagcaagc
     4741 gggacgtgcc ataataggcg tcgatatgcg cgaggacgtg gtcgcccggc gcgagcgcgt
     4801 gaaaaatcga cattgttgcg cccgttcctg aagcaaacgc cgctgccgcc gcgccacctt
     4861 caagtgcggc aattccgttt tcaagcgcct ggcggttcgg attgttgttg cgcgtataca
     4921 tgaatccgcg cgagtaggtc ccttcgatgt cgcgctcgaa cgtagtggac aagtgaatcg
     4981 gcgtggcgac cgcaccggtc gcgggatcaa tcgagtggcc ggcgtggacc gcgagtgttt
     5041 cgattctcat ttgcagcgga cgttacgatt tgaacagcga ttgtcgatct tacggcggcg
     5101 tcttttgtcg cgcgccacag acttttccct caccggcgac tttgttgtcc gcctctccct
     5161 gagggagagg attgaggtga gggcaatctg ccttgcatgc gccaacgaac gagacgtttc
     5221 gatttgacgc cccgcgccgc caagcggaaa tagcagactg cgtttcgctc gacggccacg
     5281 tggccggtct tcgtatgagc acgatttacg acgacgaggt tttcaaaatg gcgtgtgacc
     5341 agtttcaggt cattgccgat tacctcagca tcgacaaaaa cgatcgtgag cggttgatgc
     5401 tgccgaaacg cgccattgcg gtgacgctgc cggtgcatat ggacgacggc agcacgcgca
     5461 cgttccaggg ttaccgcgtc cagcatcacc tgacgctcgg gccgaccaag ggcggcacgc
     5521 ggttcgcgcc gtcattgtcg atgggcgaga cggcggccct ggcaatgtgg atgagttgga
     5581 aatgtgcgct ttcgaatttg ccctacggcg gcgcaaaggg cggcgtggcc tgcgatccga
     5641 cgaaactttc ccgcaccgaa ctcgaaggcg tatcgcgccg ttacatgcag gagatgatcc
     5701 cgtttgtcgg gccgaagacc gatatcatgg gaccggatat gggcacgaac gagcagatca
     5761 tggcctggtt catggatacg tattcggttt accaaggtta tgccgtcaat gaaatcgtca
     5821 ctggcaaacc ggtcgcaatc ggcgggaccg aaggccgacg cgaagcgacc ggccggggtg
     5881 tcgtttattt gattgagcgc gcgatgaaca tcctgaagtt cgatccggag aaatgcacgg
     5941 ccatcgtgca aggcttcggc aatgtcggct ccgtcgcagc gttgtcactt gcctataaaa
     6001 cgggaacaaa agtcgtcggg ctcagcgatg cctacgccgc gatatacaat ccaaaaggaa
     6061 tcgacgtgca ggcggcggag caacacgttt tgaaaaaagg caacctgcgc gcctttgatg
     6121 aagcaaacca tgtcgatcca aaggaattgc tgaccatgcc gtgcgacgtg cttgtgccgg
     6181 ccgccgtcga tcgcgtaatt aacggggcga acgccgggcg gttgcagtgc cgcattttgg
     6241 ccgaagcggc caatggcccg accacgccgg aagctgacct gattcttgaa aagcgttggg
     6301 atgaaatttt cgtgattccc gacattctct gcaatgccgg cggcgtaatc gtgtcttact
     6361 tcgagtgggt gcaggggttg caatcgttta ggtggtcgga atcggaagtc accgacaagc
     6421 tcttccgcat ccttgagcat tccttcgcgc aggtgatccg ccgcgcgaaa tcgcacaaga
     6481 tttcacatcg taccgccgcg atggcgatcg gcgtcgagcg cgtgttgcgg gcgaagaaga
     6541 tgcgcggctt gttcccgtga gccgtgatga gcttttcgca tcgcgaagag actcgccaag
     6601 gtcgacaact cgatgttttg acggacgagg aaaacgggat tcgcgtggtc gtctcgcgac
     6661 tcggtgctga attagtcagt ctcgcgcgaa tcaatgaaac gggcgattgg gtcggttttc
     6721 tttatcgcga caacgacatc tccgcaccgg cgcacggctg ggcaaatcac gcgacggtca
     6781 tgggctacta cctgcaccgg ttaaaggacg accgcagttt ttatcgcggg cacgaaatca
     6841 aaggtggaac gcacgggttt ttgcgacata aaacctggca ctttgctggc gctgagaacg
     6901 actcgctgac gtaccgtatt acgccagacg attttcccgc gacagattat ccgcttaaag
     6961 tcagtctcga cctcacctac aagatcgaga cgaacaaaat taccgtcacg tttgcattcc
     7021 ggaatgacga gccggagctg actgcgcacg tcggcttcgg attgcatcca ggtttcgcag
     7081 cgacttcgtt cgagagcttc cgtttgcaaa tgccgcgcgg tttgtatcgc cggcattttt
     7141 cgcccggcaa ttatctttca ggtgaaacgc gtgacctcga atttcgtggc ggcgagatgc
     7201 cattcccgcg cgaagaattg cccggctcgt tcattctgga gctcgtcgaa gtcccacggc
     7261 gcgagttttc ttatgtcgat ccgccaagtg gccgctgggt cattgtcgac cttgccgaag
     7321 ttccgtatct cacgctctgg tcggatggcg gcccgtttct ttgcctagag ccgtgctggg
     7381 gattgaccga tcaccaccag cagcgcgcgt tggaagacaa agaagggatg cagaggatcg
     7441 cgccgggcgc accactgcgc gcgtcatttt cgatggcccc gcagttcgcc tcttcggatt
     7501 gacgagaggc acgattgcgc cgttcaatct aaaatatgcc cgctgcttcg ctttacgcgc
     7561 tgctcgcgca ttccattgat tacgccggca tgtttccgcc gtgcagcctc gatttggaat
     7621 ccgcatccca caaccaagcg caatatgttc gctcgcccga tgcgtggatg cttaacagct
     7681 tcgttttgcc gatcgatcaa ttcgaagcgg ctgcgccaaa tctttcgctc ttcgacaaga
     7741 aacatgctct gcgcgtctcg gcgcttggag caaaggacgc agacttgaaa aacgcattag
     7801 aagcgattcg cgactttgcg cggcaacatg tcgatgttgt ctcggtcgtg cagttggaaa
     7861 tgccgctcgc gacagatgtc gatcttgcat ggttgcgtga agcaggcgcg accgttggtg
     7921 atttaaaact gcacgttttc tgggaagcgc cgccggatgc ggcggaggaa acgatcggtt
     7981 tgctcgcgca actgaaagag ccgcgcttcg gctataagct gcgcactggc ggggtgagcg
     8041 ccgatatgtt cccgagtgcg gttcagattg cggaggcgct ggttgccgcg acgaaacatc
     8101 acgtgccgat caagttcacc gcggggctgc atcatcccat ccgccagttc cgcgatgaag
     8161 tgggaacgca aatgcatggt tttctcaacg tgctcggcgc gggcgtgctt gcgtcggagc
     8221 atcactggga cgaaggccaa gtcgtgcaga tgctggaaga tgagcgacca gcatcgttct
     8281 ccttcgacga gacaatcttt gcctggcgcg aatgggagat tacagcggag cggattcacg
     8341 cgcgccgaaa atatgtcacc tccttcggca gctgcagttt cgacgacccg cgcgacgatt
     8401 tgcgtgctgt gggactcctt tagatcacca cggaggacac gaagctcacg aagcgtttaa
     8461 aaaacaaatt tgctcttcgt gttcttcgtg agcttcgtgg ttaaatcaca gccatgccac
     8521 tgcggtcgtt tatcgacatt gcaccggatt cgcatttccc cctcgaaaac ctgccgttcg
     8581 gcgttttcaa gccgcgcgat agcgccgtgc gcattggcgt cgcactcggt gaatacgttg
     8641 tcgatcttgc agagctcgag cgtgccggcg cgttcaaaga tctgccgccc gagacggcgg
     8701 cagctacaac gcaccgcaca ctaaatcgac tgctgccgct cggtcggccg gaatggcgaa
     8761 gactgcgtca caaactgcag catcttctct cggctgacac tgcaacgctt cgcgacgaca
     8821 cgaagctgcg ggagcgcgtc ttgcatcggc aacgggatgt cacgatgcag ctgccggcgc
     8881 ggatcgggaa ttacacggat ttctactcgt cgtatcacca tgcgcacaat ctcggcacga
     8941 tgctgcgcgg gccggagaac gcgctgatgc cgaattggaa atggctgcca gttgcctatc
     9001 acgggcgcac gagctcagtt gtgatcagcg gcacggaagt tcgccggccg cagggccagg
     9061 tcaaaccacc agacgcgccc gcgccgatct tcggcccgac caggagcctc gactacgagc
     9121 tggaaatggc gttccttatc ggcccaggca attcgttagg ccggcccgtg ccgatcgagc
     9181 gcgcggtcga tcacattttc ggatttgttt tgatgaacga ctggagcgcg cgagatatcc
     9241 agacttggga gtaccagccg cttgggccgt ttctggccaa gaatttctgc acaagcattt
     9301 caccgtgggt ggtgactctg gaagcgctcg agccgtttcg aaagcctttg ccgaagcagg
     9361 atccggagcc gctacagtat ctgcgcctaa cgaatgactt cacatacgac attcatctgg
     9421 aagcggcgct gcaaacggcg gcgaaagatc gacacgtgat cacgcgcaca aatttccaga
     9481 acctctattg gagcatctcg caacagctcg cgcatcacac ggtcggcggc tgcaatttgg
     9541 aacccggcga tctgctcgcg agcgggacaa tcagcggcgc gactgaggaa tcgcgcggct
     9601 gcatgctcga gctgacctgg cgcggcgcga agccgctgaa actgccgaat ggcgagacgc
     9661 gcaaatggct cgaggacggc gacacgctcg cgattaccgg ctggtgcgac ggcggtgatt
     9721 atcgcgtcgg ttttggcgaa gtcaccggac gcgtcgttcc agcggcggct gcttgaggga
     9781 aaccgacgaa caaaaaattt ggattggctt ttaaacgcca atgccataaa ttccgcgcca
     9841 tgtttaagac caaattcgcc ccgctgcgtt tctttctctt cacgttcgct cttgtgctgc
     9901 ccgtcgcgag ttgggcggcg aatccctacc aatccagaaa catgcatttc ggtgtttccg
     9961 ggagcaacgt gaaggatatc accagagcct attgctgcgg tggtacgctt ggttcactgc
    10021 tgagcgatgg aactacgcag tacatcttga gtaacaacca catcctcgcc cgccaagacc
    10081 gagccacgcc cggcgaaaat atttcccaac ccggattaat cgacaacggc tgccagccgg
    10141 caaccattgt cgccgacttc acggcagcgc cgaaattagg cacgaatgtg gatgcggcag
    10201 tcgcggcact gcgcgctggc aagatggact caaccggtta catcgaaggc atcggcacga
    10261 taagtagcgt gacgaaagct cccgcagtgg gactcgcagt ggaaaaaagc ggacgcacca
    10321 ctggaaccac gacgggaacg atcaactcgg ttaacaccag cgtgaacgtg cagtatcaaa
    10381 tccgctgcgg gcagggtaaa aaatacgtca tcagttacac aaatcagatc gttattaacg
    10441 gcagcggctt cagcgcgggc ggcgattcag gatcacttat tgtatccaac acgagtaact
    10501 gccatcagcc agtcggactt ctctatgccg ggagcagtac cgacacaatc gcgaatccga
    10561 ttggcgaggt cctgaacaaa gtcggaacgg cgcttgggcg atccgtcaac tttgtcggca
    10621 gcacttgcac cagttcgccc gcgcaatcgg cgcaagcaac tggagcagag gggttgcagc
    10681 ttccggaaca ggcagtcgag cacaccagta gagtgctgga gcagcatcga catgacctga
    10741 tgtcgcgtcc cggcgtcttg ggcgccggcg tcggtgcatc ggagagcggc aatgaagccg
    10801 cggtcgtcgt ttatgtcgac aacactgccg gtgcgcagcc gcagttgccg gcgcacgttg
    10861 acggggtgcc tgtgaaggtc catctcaccg atcccttcgt cgctttttag cgcaatccgc
    10921 gaagacagtt cgagcaaaac gctggcgcaa cagtaagcgg ctgaggcgat gcagccgtac
    10981 aaacggtggt cggtcgctgc atcgaattgc tgcggatcag gagtcgacgg atgaagctac
    11041 acgacatcgt atcgtcggga gttgctgcgg ccccgaagca gtcacggcga ttgaatgaag
    11101 gcggctagca tcgaggcggc cggcgaccgc tccctgaatg cggctcgatt actgaatgat
    11161 catatggacg aacgattggt gcgcacgcgg cgcgttttcc ttaagagcgg catcctcatc
    11221 ggcggcgctg cgctcgtagg tatgcacggg atgcgcggag ccgaaaagga agaggaacaa
    11281 gtgtcgcctt ccgaggatct catgcgcgag catggcgtgc tcaagcgcgt cctgctgatc
    11341 tacgacgaag cgattcggcg gatgacggca aaccaggaat tccccgtcga aacagttgtc
    11401 gattcggcaa aaatcattcg cagcttcatc gaggattatc acgaaaagct tgaggaaaat
    11461 tttctgttcc cgcgtttcga aaaggcaggc cagctaaccg atcttgtcaa gacgctccgc
    11521 gatcaacacg aagccgggcg gggagttacc gatctaaccc ttaacctggc gacacaaaaa
    11581 acgcttaaga atgcggcgga acgcccccga ctgggcaatg cgcttcgcca gttcgttcgc
    11641 atgtataatc cgcatgaagc gcgcgaagac accgtgctgt ttccggcctt tcgcaagatt
    11701 gtcagcgcaa acgaatacgc gtcgcttggg gaagatttcg agaaaaagga acatgaactg
    11761 tttggtgaag agggcttcga gaagatggtc gacaaagtga cgagcatcga aaagaagctg
    11821 ggcatttacg agctcgcgca atttacgccc aagatttaac cacaaaagcg gaatagcgaa
    11881 agcggcgctt cgattcgttg tcatcatcgg cgtcgccaat ttcttcgccg atcttaccta
    11941 tgaaggcgca cgcggaatca ccgggccgtt tctggcttcg ttaggcgcaa gcgcgaccgt
    12001 ggttggtttt gtggcgggct ttggcgagct ggtcggttac agcctgcgtt cggtgaccgg
    12061 ctatttcgcc gatcgcactc gcaagtactg gctcgttacg ttcgtcggtt acgtcatcaa
    12121 catgctggct gttcctgcgc ttgccctcgc gggcaattgg ccggtcgcgg ccggattcat
    12181 cgtcgccgag cggacgggtc gcgcgatcag aaaaccatcc gtggaggcga tgctttccga
    12241 tgccggcaaa agcatcgggt cgggttgggc attcgggcta aacgaagcgc tggaccaggc
    12301 tggcgcgatg ttgggcccat tgctgattgc actcgttttg tttcgcggcc gcaattacca
    12361 gagcgcattc gcgctgcttc tcatctccgc gattttatgc ctcggcatcg tggccgttgc
    12421 gcggctaagt catggacgca gccagtcagt ggaaagagcg gcatcgccta cgctcgcaat
    12481 gcctctgtct ttgatgccat actggattta tgtcgcggcc ggtgcgctta tcgctgctgg
    12541 gttcgctgat ttttcgctga tcgggttcca ttttcgaaaa gcatccgttg tctctgacgg
    12601 cgtcattcct atctactatg ccgcagcaat gggaacaggc gcagtcggct cattgctgtt
    12661 cggccggttg ctcgacaaac tgggccagaa agtaatcgtg ttcgtgtttc tcgtctccgc
    12721 attgttcgcc ccatgcgtat tccttggaaa ttcagtcgtg gctttcgctg gaatggcgct
    12781 gtgggggttg agcatgggag cgcaggattc gctgcttaaa gcggtgctca cacctgtggt
    12841 tccggcaggc aaacgaagcc gtgccttcgg tttgttcgac actgtttatg gcgtcgcctg
    12901 gtttctcgga agcgcgctca tgggttttct ctacgacaaa tccatcgtcg cgctggttct
    12961 gttctccgtt gtcttgcagg tcgccgcgtt acctttgctt atttgtgcaa atctccaccc
    13021 gagcaatcgc ggtcagagct aacgcttgaa agcatctccg gacaagctgc gttgcgattc
    13081 ataactcagg gatgcaaggt gacactgtag aaagcaaaac cgacaaactc cgcatcctgc
    13141 acgtggacga cgatccggat attcgcctgc tcatcgccgg ctctctacag gactttggtt
    13201 acgttgtggt caccgctgga actgtcgctg aggcgctgca attagcacga gaaatgcatt
    13261 tcaacctgtg tatcctcgac gtgcgccttc cggatggcac cgggatcgag ctatgccaac
    13321 agttacgcca gttgcagccc aataccccgg tactttatta ctcggcgtat gctagcgatt
    13381 cggaacaaaa ggaagcgctt tccgtctgcg gagatgcgta tttgaaaaag ccggtgttgg
    13441 ccgaggaact ggagcaaacg attacccggc tgctcaatcg cgagcaacaa gattgaacac
    13501 gtctctgcct gatgcttaac gggcgataat tgacttgata ttactgcgcg gtctaagtca
    13561 tttcgcgaat ctggcggcca caaccgagcg cgaataaact gcgcagcggc gcagcgcttg
    13621 ctcctttttt tgcgcgcgat gcaactgtgt gcgattcatc ccgcgatttt gtctgcgcgg
    13681 atatcttatg ccggaatttc cctgcgcatc gaattcacga tcgtgaagac cgcgcctgct
    13741 cgctaattac ccatgaagaa aaccagttct gcaccagtca cgcggccggg aaacggcgcg
    13801 cggtttaacg gcagtgaagt ggatgccaaa cagttgctta cggcattgat cgcattcaaa
    13861 cgcggcgatt tttcggcgcg cttgcccgac gactggaccg gcgtggccgg gaagatcgcc
    13921 gatacgttca acgacgtaat cgcgacgaat cagcgcatga ctcgggaact ggagagaatc
    13981 ggacgcgttg ttggaaagca aggcagaatt actcagcgtg catcgctggg cgacgtatcg
    14041 gattcatggg ctgatgcaat tggttcggtc aacgatctga tcggcgacct ggtccagccg
    14101 accagtgaaa tggcgcgtgt catcggcgcg gttgcgaaag gtgatttgtc gcaaacgatg
    14161 gcaacggaaa tcgaaggccg cccgctcgaa ggagaatttt tgcgcacggc aaaggtggta
    14221 aacacgatgg ttgatcagct tggcgccttc gcctcggaag tgacgcgggt ggcgcgcgaa
    14281 gtgggtaccg aaggcaagct cggcggccag gccaaagtca aaggtgttgc cggcacgtgg
    14341 aaggatttga ccgagaacgt caaccttatg gccggcaatc tcacggcgca ggtgcgcaac
    14401 atcgcgaccg tgacgacggc ggtggcgaac ggagatttga ccaagaaaat cactgtcgat
    14461 gttaaaggcg agttcctgga gctgaaagac acagtcaacg tgatggttga tcagctgcgc
    14521 tcgttcgcgt cggaggtgac gcgcgtggcg cgggaagtgg gctcggaagg aattctcggc
    14581 ggacaagcgc gcgtcgaagg tgtttccggc acatggaagg acctgactga ctcggtcaat
    14641 tttatggcgc gcaatttgac cggacaggtg cgcaacatcg ctgcggttac gactgccgtc
    14701 gcacgcggtg atctgtcgaa gaagattacg gtcgatgtga aaggggaaat tctcgaactg
    14761 aagaacacga tcaacacgat ggttgatcaa ctcagctcgt tcgcctcgga agtgacgcgt
    14821 gtcgcgcgcg aggtgggcac cgagggaaaa ctcggcggcc aggccgatgt caaaggcgtc
    14881 gctggaacgt ggaaagatct gaccgacagc gtcaactcga tggccggcaa cctgaccgcg
    14941 caagtgcgaa acatcgcgga cgtgacgaca gcggtcgcga ccggtgattt gtccaaaaag
    15001 atcactgtcg atgtccgtgg tgagatcctc gaactgaagg acacgatcaa caccatggtc
    15061 gatcagcttc gttcctttgc ctcggaagtg acacgtgtcg cacgcgaggt cggccgcgag
    15121 ggaaaactcg gcggtcaggc ggatgtgcgt ggcgtagcag gcact
//
LOCUS       DASTDG010000211         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1664636, whole genome shotgun sequence.
ACCESSION   DASTDG010000211 DASTDG010000000
VERSION     DASTDG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664636"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VFO30_07010"
     CDS             <1..114
                     /locus_tag="VFO30_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9419075.1"
                     /translation="YDIRTVQELLGHKDVSTTMIYTHVLNKPGIGVKSPLD"
     gene            complement(274..975)
                     /locus_tag="VFO30_07015"
     CDS             complement(274..975)
                     /locus_tag="VFO30_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419076.1"
                     /translation="MRNSAARSELDSMVVEIELTLVSIIQGVALTILIENARDIVAQR
                     AFLFWPYVLAGLVMIFIFWSRAVLHIITVIRWPLEFGHNFLYIGCALVEALMFAQLAR
                     PRNWFAFGAAFIAVGWALFIYDLRLIYARMRDSAGPASDRLCAMVKRDQWLNIAVLLP
                     GVLLLNGASVLLIHAHPGFFIGRNGHLWLVAAQLAGFGGYLLYVVRFYSKLAPFISEA
                     RAEWRATTAAKPDND"
     gene            complement(1042..1115)
                     /locus_tag="VFO30_07020"
     tRNA            complement(1042..1115)
                     /locus_tag="VFO30_07020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1079..1081),aa:Arg,seq:tcg)
     gene            complement(1237..1383)
                     /locus_tag="VFO30_07025"
     CDS             complement(1237..1383)
                     /locus_tag="VFO30_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419077.1"
                     /translation="MLIVTGTARGEIAGELSDFARLLADCDIFLTLDGAWIFFRPEFI
                     RLAL"
     gene            complement(1410..1484)
                     /locus_tag="VFO30_07030"
     tRNA            complement(1410..1484)
                     /locus_tag="VFO30_07030"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:His,seq:gtg)
     gene            1634..3751
                     /locus_tag="VFO30_07035"
     CDS             1634..3751
                     /locus_tag="VFO30_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9419078.1"
                     /translation="MKKVGAILIAGLFLICALTRLPAQSEDPSDIFLKAYMTSQQAEK
                     LEHENEFKAALAKYRAAGALIEQLHKNYADWQPAIVEYRGRKISESILRVQDKAGTQS
                     DLAGTSAPPEENAPPPSTAVPQPSAVITTARPSAAPPQVANDSAIKEATKKLNDKIDQ
                     LQSELRKSRSQLETAQKEKESLNGRLNDTTTKLTQAHGELEKSKTAERQGRDQLARAE
                     QSLKKIESSPGASTKAQEALRAEITRLKDALTAADEARLAAEKEKEQRGAKLAEAERK
                     IVTVTQERDQAVAQLKGGKESEQRVQMLVAENSDLKKKLADAEKSMREISAGKPQKEQ
                     ELVEVKGQLEQLRTQLLASQKQNHDYELKVADLRSQLDDMASQLEKAKLKGATNEETI
                     RLTKENEMLRNIVVRERQEEARRDQAKKLLLAEFDKLRIKSDTLNEQIQLLAQPITKL
                     SDQELALLRQPVVSISDQNPAAMKASFTFAKKSAMNAVNIAGSEGNAPPGAETQAGQP
                     SGANANSGFNPDVPEEVKPVAREAKQNFEQRKYRAAEKQYQQILTKSPNNLYSLCNLG
                     VVYFRTARFKAAELTLKKAAAVAPQDEFAHTTLGIVYYRQSKFDDALAELTKALAINP
                     KSATAHNYLGITASQKGWQEAAEREILEAIADNPDYADAHFNLAVIYATQRPPAKELA
                     KQHYAKATQLGTEPDASLEKLLH"
     gene            complement(3898..>6206)
                     /locus_tag="VFO30_07040"
     CDS             complement(3898..>6206)
                     /locus_tag="VFO30_07040"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9419079.1"
                     /translation="PDTLVGTDSHTTMIKGLGVVGWGVGGIEAEAGMLGQPVYFLTPE
                     VVGVHMTGQLREGVTATDLVLHMTQLLRAQKVVGKFVEFYGEGAASLPVPDRATIGNM
                     SPEYGATMGFFPIDSESVAYLRATGRSEEQCTAFENYFRAQNMFGMPRKGEIDYSVDL
                     DVDLADVQPSVAGPKRPQDRINLPELGQTFRTLLTKPVTDGGYGKQDVDLAAKYVVQL
                     NGSAPNDGDMPSSDKIGPSIEPGEERNKAEMVNNRPTPDTAKELSDSTADPFHHGESR
                     IGHGSVLIAAITSCTNTSNPSVMLAAGLLAKKAVERGLHIDPAVKTSLAPGSRVVTDY
                     LDKTGLQKYLDQLGFNLVGYGCTTCIGNSGPLHPNIEKAIHNYDLVAASVLSGNRNFE
                     ARVHQNIKANFLMSPPLVVAFALAGRVDVDLSKEPIGKDKNGKETFLRDLWPSLKEVR
                     DAMRAALKPETFRKLYRDFAEQNPKWNEIPSSTGEVYQWDERSDYIHEPPFFKDFSME
                     PGKIDNIVGARVLGIFGDTVTTDHISPAGAIKASSPAGQYLISRGIQPQDFNSYGSRR
                     GNDLVMTRGSFANVRIKNLMVPGTEGGVTKHYGDASVPLAKEGKPEAYATSGEQMSIF
                     DAAMRYQKENVPLVVIAGHEYGTGSSRDWAAKGTRLLGVRAVVAASFERIHRSNLVGM
                     GVLPLQFHDGTNAQTLELQGSEILSVTGLSDALEPGQEVTLEILGKDRQSRSVPVKLR
                     IDTPIEVDYYRHGGILPFVLRQLLSRKQ"
BASE COUNT         1446 a         1757 c         1826 g         1177 t
ORIGIN      
        1 tatgatattc gcaccgtgca ggagttgctg ggtcacaagg atgtgagcac gacaatgatt
       61 tacactcacg tgctcaataa gccaggtatc ggtgttaaga gtccgctgga ctagttaatt
      121 gaggattgcg gaaagcggat aagaaaggcg agccaagtcg agcatgagca tgattatgag
      181 catccgcctt cgctgtcggc tgcggcgcga caagtgagca ggagcgccca ggattgcgga
      241 aagcggatac gggataatcg ccgtcgattg ttttcagtcg ttgtcgggct tcgcggctgt
      301 cgtggcgcgc cattcggcgc gtgcttcgga gatgaacggc gccagttttg aataaaaccg
      361 caccacatag agcaaataac cgccgaaccc ggcaagctgt gccgcgacaa gccagaggtg
      421 accattgcgt ccgatgaaaa accccgggtg tgcgtggatc agcaagactg acgcaccgtt
      481 caagagcaat acgccaggca gcaaaaccgc gatgttcagc cattgatcgc gctttaccat
      541 cgcgcagagc cgatcgctcg ccgggccggc actgtcgcgc atccgcgcgt aaatcaggcg
      601 caaatcgtaa ataaagagcg cccatccgac ggcaatgaag gcggcgccga aggcaaacca
      661 attccgcggg cgcgccagtt gggcaaacat gagcgcttcg acaagtgcgc agccgatgta
      721 gagaaaatta tgtccgaact caagtggcca gcgaatgacg gtgatgatgt gcaagaccgc
      781 gcgcgaccag aaaatgaaga tcatgaccaa gccggcgagc acatagggcc agaatagaaa
      841 tgcgcgctgc gccacgatat cgcgcgcgtt ctcgatcagg atggtgaggg ctacgccttg
      901 aatgatgctg acgagggtga gctcgatctc gaccaccatt gagtcgagct ccgaacgcgc
      961 cgcggaattt ctcattgcgt aagatcgaca tcagaaagaa gggcgctctg caatttgaaa
     1021 tttgcaaccg gcgattatct ccgtgcctgg agggattcga acccccaacc ttctgatccg
     1081 aagtcagatg ctctatccag ttgagctaca gacacagggc gattgccgat tgtcgattgc
     1141 cgattgtcga ttgcaagatc gacattgtgc tgtaggggcc gcttgtatcg agcgtcgaac
     1201 ggaacgacgc acaataggcg cttggcacaa gcgcctctac aacgccaggc gaatgaattc
     1261 gggccggaag aaaatccaag cgccgtcgag cgtgagaaag atgtcgcagt cggcgagcag
     1321 tctcgcgaaa tcgctgagct cgccggcgat ctcgccacgc gcggtccccg tcacaatcag
     1381 caatcggcga tcggcaatcg aaaatgaatt ggggtggccg acgggactcg aacccgcaac
     1441 aaccagaacc acaatctggg gctctaccat tgagctacga ccaccagctc agggcgaaga
     1501 gtttaatttc agcagggcgg attgcaaagg aaagcactgg ggcttttggc acattctccg
     1561 ctacgctggg gagggcgtgg aaggctgtat tgcaactgga tactcctctg gtatcgttcc
     1621 ggctcgccct cgcatgaaaa aggtcggtgc cattttgatt gccgggctat ttctcatctg
     1681 cgcccttact cgtctgccgg cgcaatcgga agatccgagt gacattttcc tcaaggccta
     1741 catgacctca cagcaggcgg aaaagctcga gcacgagaac gagttcaagg cggcgctcgc
     1801 caaatatcgc gccgcgggtg cgttaatcga gcagttgcac aagaactacg ctgactggca
     1861 gccggcgatc gttgagtatc gaggccggaa aatcagcgaa agcattctgc gcgtgcagga
     1921 caaggccggg acgcagagcg acttggctgg gacatctgcc cccccggaag agaacgcgcc
     1981 gcctccatca acagcagtgc cgcaaccaag cgctgtaatc acgacggcac ggccgagcgc
     2041 ggcgcctccg caggtagcga acgactccgc catcaaggaa gcgaccaaga agttaaatga
     2101 caagatcgac cagttgcaga gcgagctacg gaagtcgcgg agccaattgg agacggcgca
     2161 gaaggagaag gaatcgttaa atggtcgctt gaatgacacg accacaaagc tgactcaggc
     2221 gcacggtgag ctcgaaaagt cgaagacggc agagcgccag ggccgcgacc agcttgcgcg
     2281 cgccgagcaa tcgcttaaga agattgaaag ttcgcccggc gcgagcacaa aggcacagga
     2341 ggcattgcgc gccgagatca cgcggctgaa agatgcgctg accgccgcgg acgaagcgcg
     2401 gctggcggcc gagaaggaaa aggagcagcg aggcgcgaag ctggccgaag cggaaagaaa
     2461 gatcgtcacc gttacacagg aacgcgatca ggcggtcgcg cagttaaagg gggggaaaga
     2521 atccgagcaa cgggtgcaaa tgctcgtcgc cgagaacagc gatttgaaga agaaactcgc
     2581 ggacgcggag aagagcatgc gcgagatcag cgcgggcaag ccgcagaagg aacaggagct
     2641 ggtggaggta aaaggacagc tcgagcagct gcgcacccaa ttgcttgcga gccagaagca
     2701 aaatcacgat tacgagctca aggtagccga tctgcgctcg cagttggacg atatggcgtc
     2761 gcagctcgaa aaagccaagc tcaaaggcgc gacgaacgag gagacgattc ggctgacaaa
     2821 ggaaaacgag atgctgcgca acatcgtcgt gcgtgagcgg caggaggaag cgcgtcgcga
     2881 ccaggcgaaa aaactcctcc tggccgagtt cgataagttg aggattaaat ccgacacgtt
     2941 gaacgagcag atccagttgc tcgcacaacc gatcacgaaa ttaagcgacc aagaactcgc
     3001 gctgttgcgg cagccggtgg tttcgatttc cgatcaaaac ccggccgcga tgaaggccag
     3061 tttcacgttc gcgaagaaat cggccatgaa cgcggtgaac atcgccggat cggagggcaa
     3121 cgccccgccg ggcgctgaaa cccaggccgg ccagccttca ggcgcgaacg cgaacagtgg
     3181 gtttaatccc gacgtgccgg aggaagtgaa accggtcgcg cgtgaggcga agcagaattt
     3241 cgagcagcgc aagtatcgcg cggcggaaaa gcagtaccag cagatcctga cgaaaagccc
     3301 gaacaacctg tattcacttt gcaatcttgg cgtggtctac ttccgaaccg cccgattcaa
     3361 ggctgcagag ttaacgctca agaaagcagc cgccgttgcg ccgcaggacg aattcgcgca
     3421 tacgacactc ggcatcgttt attaccggca atcgaaattc gacgacgcgc tcgccgagct
     3481 gaccaaagcg ctcgccatta acccgaaaag cgccaccgcg cataattatc tcggcattac
     3541 cgcgagtcaa aagggctggc aggaagcggc cgaacgcgaa atcctcgaag ccatcgccga
     3601 taatcccgat tacgccgatg cgcatttcaa tctggcggtg atttacgcga cccagcgtcc
     3661 gccggccaaa gaactggcga aacaacatta cgccaaagcg acccaactcg gcactgagcc
     3721 ggacgcttcg ctcgaaaagc tgctgcactg agccaacgcc agcgggccct cacctctcgc
     3781 cacggcgagt tcgtccggtg gcgaacagtc ctctctcgct cgtaatgtgt gcgcacgacg
     3841 tacggcaaag tccctccgtt tcggttactg tcattccgag cggagtcgag gaatctctta
     3901 ctgcttccta ctgagcagtt gccgcaaaac aaacggcaag atcccgccgt gtcggtagta
     3961 atcgacttcg attggggtgt cgatccgcaa cttcaccggg acggagcggg attgtcgatc
     4021 tttgccgagg atctcaagag tgacttcctg acctggctcg agcgcgtcgg ataatcccgt
     4081 gaccgacaag atctccgatc cctggagctc gagtgtttgc gcgtttgttc catcatggaa
     4141 ctgcaacggc aacacgccca tcccaaccag attcgagcgg tggatccgct caaagctcgc
     4201 cgcgaccacg gcgcgtacgc ctaacaaacg cgtgcccttc gccgcccaat cccgcgatga
     4261 ccccgtgccg tactcatgtc ccgcgatcac gaccagcggt acgttctctt tttggtagcg
     4321 cattgctgca tcaaagatcg acatctgctc gcctgaagta gcgtaagcct ctggcttgcc
     4381 ttcttttgca agcgggacgc ttgcgtcacc atagtgtttc gtcactccgc cttcggttcc
     4441 aggcaccatt agatttttaa tccgcacatt cgcaaagctg ccgcgcgtca tgaccagatc
     4501 attcccgcgg cgcgagccgt agctgttgaa atcctgcggt tggattccgc gcgaaatcag
     4561 gtactgccca gccggagaac tcgctttaat agcgccggcc ggcgagatgt gatcggtcgt
     4621 taccgtatca ccgaaaatgc cgagcacgcg cgcgccaacg atattgtcga tcttgccggg
     4681 ctccatcgag aaatctttga agaagggcgg ttcgtggatg tagtcgctcc tctcatccca
     4741 ctgataaact tcgcccgtgc tcgaggggat ctcgttccat ttcgggttct gctcggcgaa
     4801 atcgcgataa agtttgcgga aagtttccgg cttcagcgcc gcgcgcatcg catcgcgcac
     4861 ttctttcaaa ctcggccaga gatcgcgcag aaaagtttct ttgccgtttt tgtccttccc
     4921 gatcggttcc tttgaaagat cgacatcgac ccggcctgcg agcgcgaacg cgacgaccag
     4981 cggcggcgac atgagaaagt tcgctttgat gttttggtgg acgcgcgcct caaagttgcg
     5041 gtttcccgaa agcacagacg cggcgacgag atcgtaatta tggattgctt tctcgatatt
     5101 cggatgaagc ggtccggaat tgccgatgca agtcgtgcag ccgtagccga ctagattgaa
     5161 gccgagctga tcgagatatt tttgcaggcc ggttttgtcg agataatcgg tgacgacgcg
     5221 cgagccggga gcgagcgacg ttttcacggc cggatcgata tgcaggccgc gttcgaccgc
     5281 tttcttggcc agcagaccag cggcgagcat cacgcttgga ttgctcgtgt ttgtgcagct
     5341 ggtgatcgcc gcaatgagga cgctgccgtg gccgatccgg ctttctccgt ggtgaaaggg
     5401 gtcggccgtc gaatcgctca attccttggc tgtgtccggc gtcggccggt tgtttaccat
     5461 ctcggccttg ttgcgctctt cgcccggctc gatgctcggt ccgatcttgt cggatgaagg
     5521 catatcgcca tcattcggcg cgctgccgtt caactgcaca acgtatttcg ctgcaagatc
     5581 gacatcctgt ttgccgtagc cgccgtccgt caccggcttt gtcaacagcg tgcgaaacgt
     5641 ttggccaagt tctggaagat tgattcgatc ttgtggacgt ttcggcccgg ctacgctcgg
     5701 ctgcacgtcg gcaagatcga catcgagatc gacgctgtaa tcgatctcgc ctttgcgcgg
     5761 catgccaaac atgttttggg cgcgaaagta attttcaaac gcagtgcatt gctcttcgct
     5821 gcgtccggtt gcacgcaagt acgcgaccga ttcgctgtcg attgggaaaa agcccatggt
     5881 ggcgccgtac tcgggcgaca tgttgccgat cgtcgcgcga tcggggacgg gcaatgatgc
     5941 cgcgccttcc ccgtaaaact cgacaaattt gccgacgact ttctgcgcac gcaacaattg
     6001 agtcatgtgc aaaacgaggt cagtcgcggt gacgccttcg cgcaattggc cggtcatgtg
     6061 cacgcctacg acttccggcg ttaagaaata aaccggctga ccgagcatgc ccgcttcagc
     6121 ttcgattcca cctacgcccc agccgacgac gccgaggccc ttgatcatgg tggtgtgcga
     6181 atccgttccg acgagcgtgt ccgggt
//
LOCUS       DASTDG010000212         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1048595, whole genome shotgun sequence.
ACCESSION   DASTDG010000212 DASTDG010000000
VERSION     DASTDG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048595"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2325
                     /locus_tag="VFO30_07045"
     CDS             127..2325
                     /locus_tag="VFO30_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9419080.1"
                     /translation="MSFGAPEWFWAVLIVPALVALYANAERRAAQRLREFVSPRLLPQ
                     LAATIDRRRRAWRFALQLLGLALAIVSLAQPRWGYSFEDVKRKGLDLLLAVDTSRSML
                     SNDVAPNRLERVKLATQDLINQLQGDRVGLIAFAGRAFLQAPLTIDYEAAVESINDLD
                     TKTIPEGGTNISEAISLATQTFGKSATGNRALIIFTDGEELSGDAVKTAKSAADAGMH
                     IFTIGVGTPQGSLIPIASEGDGTPFVKDANGQVVKSKLDEKRLQEVAQIGGGLYLHLE
                     NGPRTMQQLYTDGLAKLNAAEMDVRLSRRPIERYEWLLGLALLALTLSMLIPERKRAR
                     AQVYSGMPAKAAAAGAAILLCSGVNGLASVPALDAYRDGKFEDAYREFQETLKSHPNS
                     PAEDKLQFDSGAAAYKLKDYGKALEAFSQALLSPDAQLQSMSHYNLGNTLYQRGESQQ
                     SDDKKLTDWTNALQHYEQTLRLEPQNQEARDNYEYVKKKIEELKKKKEQQPSSSPSPP
                     PQSKPQKNQDQNQQQQQNQQQQSSSQDQQQQDQNQQQQQQQQAQNKPEAGQNQKPEDK
                     QDQAQAQNGPQKNQQQQSGETPSPSPGEEQQQPPKPSAGEQKQNQPGQMPSPGERQAE
                     ESPTPGEGENETPSPSPGEGEGAEESPGPSATPTESPQKKMAGEIKGAPGEKPEKPPD
                     KAAEPADIDSEKRGEMSERQAEALLQSMKDEEARVRLDEHQRARRVYNDW"
     gene            2365..4212
                     /locus_tag="VFO30_07050"
     CDS             2365..4212
                     /locus_tag="VFO30_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HET9419081.1"
                     /translation="MSILRQILLGGFVPVAVALHAFADSPNVTAVLSNSEAAVGEMVQ
                     LQIKVSGSGAKPPAEINIDGLDIRRTGTEQRYEMNNFNVSQSIVYDYTILPTRAGTFK
                     IPPQPVRFANQTLRTPELTLHVSDSTAGSATARAPRSSGSIDTSKLVFAELIVPKKTA
                     YVGEMVPVEIRLGFDPRVRPKLVDGPEITGQGFTAQKLHQAGENLETINGKTYDVVTF
                     KSAVAAARPGKFQIGPVTAKAQVSVARRSGAPRSRSPFDLFSLDDPFADPFFADPFGR
                     LAERREVDLQSEPVEFEVKPLPPNAPPGFTGAIGNFTLNTEAKPTNAQVGDPITVRST
                     ISGRGNFDRVNAPELQDERGWHKYPPTASFKHDDDVGISGAKTFEMVISPNENKRAIP
                     PLVFSFFDPAKEKYVTLNSQSIAVRISGAPAQPVTAPSTVPSPAPAMTPAAAAVTRAA
                     PRSQDILYQLNERGRTASFTPLFLQREFWLAQIGPLIVLLGFIAWQIRQRRLDNREAR
                     RIAALQHESAELLRKLRRSDLSPMEYFSDAARAVRLKTALAKDVDPNAVDAEMAASVF
                     ALDEASRAQLRRLFERNDELRYSGWPNGAGRVNGEDREQVLELLESLRT"
     gene            4209..>4882
                     /locus_tag="VFO30_07055"
     CDS             4209..>4882
                     /locus_tag="VFO30_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419082.1"
                     /translation="MKIRLIVDLSCAVICLLWVRPTFAQPDAEFSKATQEYSAGHFQE
                     AINGYEALVRSGEWSANLFYNFGNAYFRVADFGHAILNYERALALERHHPEAEANLRI
                     ARDQARALELAPAAIERALRFASVNEFAIAAAIAFWVTAFSLVWLLVARRRGAGMIAL
                     LIFSALIFALAAFASYQLENGAKGKALAIVVANNVEARLATADSAQTVLALPPGSEIQ
                     ILSTRG"
BASE COUNT         1150 a         1407 c         1428 g          897 t
ORIGIN      
        1 tgctggtcgg gcagattctg ttgtcgcaaa cgatttggaa gaaactgccg tgacgaagat
       61 gtcgatcttg caacgtggca tgggcttcca gcccatgtcc catcggcaag atgccgatgc
      121 cacgttatga gctttggtgc accagaatgg ttttgggcgg tgctgatcgt gccggcgctg
      181 gtcgcgttgt atgcgaatgc agagcgtcgt gcggcgcagc gactgcgcga gttcgtatcg
      241 ccgcgtctgc tgccgcaact cgccgcgacg atcgatcgtc gtcgacgtgc ctggcgtttt
      301 gcactccagc tattgggtct agcgctggca attgtgagcc tggcgcaacc gcgttggggc
      361 tactcatttg aggacgtaaa gcgtaaagga ctcgacctgc tgctcgcggt cgacacgtcc
      421 cgcagcatgt tatccaatga tgttgcgccg aaccggctgg agcgcgtcaa actcgccacg
      481 caagacctga ttaatcaact gcaaggcgac cgtgtcgggc tcatcgcatt cgcagggcgc
      541 gcatttttgc aggcgccgct gacgatcgac tatgaagccg cggtcgaatc gatcaacgat
      601 ctcgacacca aaacgattcc cgagggcggg acgaacattt cggaagcaat ttcgctcgcg
      661 acacaaacat tcggaaagag cgcgacggga aatcgcgctc tcatcatttt caccgatggc
      721 gaggagctga gcggcgatgc cgtgaaaacc gcgaagagtg ccgcggacgc cggcatgcat
      781 attttcacga ttggcgtcgg aacgccacag ggctcgctca tcccaatcgc cagcgaaggc
      841 gatgggactc cgttcgtcaa agacgccaac gggcaagtgg tgaaatcgaa gctggatgaa
      901 aaacgtttgc aggaggtcgc acaaatcggc ggcggacttt atctccatct cgagaacggg
      961 ccgcgcacga tgcaacagct ttacactgat ggcttggcga agttgaacgc cgcagagatg
     1021 gacgtccgtt tatcgcgccg gccgatcgaa cgttacgagt ggctcctcgg tctggcgctt
     1081 ctcgctctca cattgtcgat gttgattccg gagagaaagc gagcgcgagc gcaagtctat
     1141 tccgggatgc cggcgaaagc ggcggcggct ggagccgcta ttctcctttg ctctggcgta
     1201 aatgggcttg cgtctgttcc cgcacttgat gcctatcgcg acggaaaatt cgaagatgcc
     1261 taccgcgaat ttcaggagac gcttaaatcg catccgaatt cgccggccga agacaagctc
     1321 cagttcgatt ctggcgcagc tgcgtacaag ctaaaggact acggcaaagc gctggaagct
     1381 tttagccagg cgttgctttc accggacgca cagttgcaga gcatgagtca ttacaatctc
     1441 ggcaacacgc tttatcagcg cggcgaatcg cagcagagcg atgataaaaa attaaccgat
     1501 tggaccaacg ctttgcagca ttacgaacag acgctcaggc tcgaaccgca aaaccaagag
     1561 gccagggaca attacgagta cgtcaaaaag aagatcgaag agctgaagaa aaagaaggag
     1621 caacagccgt cgtcgtcgcc ttcaccgccg ccgcaatcaa aaccgcaaaa gaaccaggac
     1681 caaaaccagc aacaacaaca gaaccaacaa cagcaatcgt catcgcaaga tcaacaacaa
     1741 caggatcaga accagcagca gcaacaacaa caacaagcgc agaacaaacc tgaagctggt
     1801 caaaaccaaa aaccggaaga caagcaggac caggctcagg cgcagaacgg gccgcaaaaa
     1861 aaccagcagc agcagtcagg cgaaacgccc tccccttccc ctggagagga gcaacagcaa
     1921 ccgccgaagc cgtcagccgg agagcagaaa caaaaccagc ccggccagat gccgtcgccg
     1981 ggcgagcgac aagcagagga atcccccact cctggcgaag gtgaaaatga aacgcctagt
     2041 ccgtcgccgg gagaaggtga gggcgcggag gagagtcctg gcccatctgc aactccaacc
     2101 gaatcgccgc agaagaaaat ggcgggcgaa atcaaaggag caccgggcga aaaaccggag
     2161 aagccgccgg acaaagcagc tgagccggcc gacatcgaca gcgaaaaacg aggcgagatg
     2221 agcgagcgac aggccgaagc gctgctgcaa tcgatgaaag acgaggaagc acgcgtccgg
     2281 ctggatgaac accagcgtgc acgccgtgtc tataacgact ggtaaaagga cgtttgatgt
     2341 ttgaggtttg atgtctgatc cacgatgtcg atcttgcgcc agatcttatt gggcggcttt
     2401 gtcccggtcg cggttgcact tcacgcgttc gccgattcgc cgaacgtcac ggctgtcctg
     2461 agcaactccg aagccgccgt cggcgagatg gtgcagttgc aaatcaaagt cagtggttcc
     2521 ggcgcgaagc cgccggccga gatcaacatc gacggtctgg acattcgtcg gacgggcacg
     2581 gaacagcgct acgagatgaa caatttcaac gtctcgcaaa gcatagtcta tgattacaca
     2641 atcctgccga cccgcgccgg cacgttcaaa atcccgccac aaccggtccg tttcgctaat
     2701 caaacactgc gcacacccga attgacgttg cacgtgagcg actccactgc cggatcagcg
     2761 acggcgcgcg caccgcgcag ctccgggtca atcgacacga gcaagctggt ttttgccgaa
     2821 ttgatcgtgc cgaaaaagac tgcctatgtc ggcgaaatgg tgccggtcga gattcgactc
     2881 gggtttgatc cacgtgtgcg accgaaactg gtcgacggtc cggaaatcac cggtcagggg
     2941 ttcaccgcgc agaagctcca tcaagcggga gaaaacctcg aaacgatcaa cggcaaaacc
     3001 tatgacgtgg ttacgtttaa gagcgccgtc gcggccgcgc ggcccggcaa atttcagatc
     3061 ggaccggtga cggcaaaagc gcaagtatct gtcgcgcgtc ggtctggtgc gccgcgttcg
     3121 cgctcgccgt tcgatttgtt cagcctcgat gatccgtttg cggacccatt cttcgccgat
     3181 ccattcggtc gtctcgccga gcgtcgcgaa gtcgatctgc agagcgagcc ggtcgagttc
     3241 gaggtgaaac cgctgccgcc gaatgcgccg ccgggattca ccggcgcgat cggcaatttc
     3301 acgctcaaca ccgaagccaa gccaacgaac gcgcaagtcg gcgaccccat tacggtaaga
     3361 tcgacaatca gcggccgcgg caatttcgat cgggtcaacg cgcccgagct gcaggatgag
     3421 cgcggctggc acaagtatcc gccgaccgct agtttcaaac acgacgacga tgtcggcata
     3481 agtggagcaa agacgttcga aatggtgatt tcacctaacg aaaacaagcg cgccatccca
     3541 ccgcttgttt tttcgttctt cgatccggca aaagagaaat acgttacgct caatagccag
     3601 tcgatcgcgg tgaggatttc gggcgcacca gcccagccgg ttacggcccc aagtaccgtc
     3661 ccatcgccgg cgccggccat gacaccagcc gcagcggccg tcacccgcgc ggctccgaga
     3721 tcgcaggaca ttttgtatca actgaatgag cgcggccgca cagcctcctt cacgccgctt
     3781 tttctccagc gcgagttctg gctggcgcaa attggaccgc ttattgtgct gcttggtttc
     3841 atcgcatggc agattcggca gcgtcgcctg gacaaccgtg aagcacgccg cattgctgcg
     3901 ctgcagcatg aatcggccga actcctccgc aaactccggc gaagcgatct ttcgcccatg
     3961 gaatatttct ccgacgccgc gcgcgcagtt cgcctcaaaa ctgcgctcgc caaagatgtc
     4021 gatccaaatg ccgtggacgc agagatggcg gcgtcggtat ttgcgcttga tgaagcttcc
     4081 cgcgcgcaat tgcggcggtt atttgagcgc aacgacgagc tgcgttacag cggctggcca
     4141 aacggcgccg gaagagtgaa tggcgaagat cgcgagcaag ttctggagct cctcgaaagt
     4201 ctccgcacat gaaaatcaga ctgattgtcg accttagctg cgccgtgatt tgcttgctgt
     4261 gggttcgacc aacattcgcg cagccggacg ccgagtttag caaagcgacg caggagtact
     4321 cggcaggcca ttttcaggag gcgatcaatg gatacgaggc actcgtccgc tccggcgagt
     4381 ggagcgccaa tctgttttac aatttcggta acgcctactt tcgcgtcgcc gatttcgggc
     4441 acgcgatttt gaattacgaa cgggcgctgg cactcgagcg gcatcatccg gaggcggagg
     4501 caaatttgcg gatcgcgcgc gatcaggcgc gcgcattgga gctcgcgccg gctgcgatag
     4561 agcgggcgct ccgtttcgcg agtgtcaacg agttcgcgat cgcggcggca atcgcctttt
     4621 gggttacagc gttttcactc gtctggctgc tggttgcgcg ccgacgcggt gccggcatga
     4681 ttgcattgtt gattttttcg gcgttgatct tcgcgctggc ggcgttcgcc agttatcaac
     4741 tggagaacgg tgcaaaaggc aaagcattag cgattgtagt tgccaataat gtggaggcgc
     4801 gattggccac ggccgacagc gcgcagactg ttcttgcctt accgccaggc agcgaaatcc
     4861 aaattctgag cacccgcgga ga
//
LOCUS       DASTDG010000213         5724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_669761, whole genome shotgun sequence.
ACCESSION   DASTDG010000213 DASTDG010000000
VERSION     DASTDG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669761"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFO30_07060"
     CDS             complement(<1..1286)
                     /locus_tag="VFO30_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9419083.1"
                     /translation="MASSDRNSNRENKSRGNEPNFNWRGVILIVIAFAFIGLAMLFYR
                     GGMQPVEEVPYNRFLELLENKQIVNDKNFPLTLIVEEGRATQALSGYYLRQSVGSQPA
                     QPVKFRTTIYLNFTNNLQDKLNAAGIQPAIKTESNVIAQTVAGFLPIALFLLVLYFLF
                     RQQIRMAGKGALNFGKSKARMLARDKNKITFRDVAGVEEAKDEVQELVEFLRDPKKFQ
                     KLGGRIPKGVLLVGPPGTGKTLLARAIAGEADVPFFSISGSDFVEMFVGVGASRVRDM
                     FEQGKKSAPCIIFIDEIDAVGRHRGHGVGGGHDEREQTLNALLVEMDGFDTQEGVIII
                     AATNRPDVLDPALLRPGRFDRQITVNLPDVKGREEILRVHSKKVKLAEGVDLAVVARG
                     TPGYSGAELANVINEAALLAARRGLKGITLAELEEAR"
     gene            1477..5085
                     /locus_tag="VFO30_07065"
     CDS             1477..5085
                     /locus_tag="VFO30_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9419084.1"
                     /translation="MTELVSTVADKIDRAVQKVTPFAYRFRRRGRRLMRRALRKPRSV
                     SEGPNLLPLLIQVLASFTKADGVLLEEEIDSSLGFLRYDYPEAVYSELRELFRQALYE
                     QQDLAAMAQKLGAQLSTDRKIMLGVQLYDLISQAGLKQEQVVQFYSFMSQLGMAAQAI
                     DIVYQLNASEDSDPAIYQRGASPLESLSFAANGKADVTLRTLAGNDRLLAFRYHDLIL
                     LKNYSTQNVSVRGRPLVRGGFCRIYPGQRILVGDQVLTYQDLATYFNAKKNVSMPHVF
                     VRVNKDADEVALERARSRESALEITFGLKVRVKALRDVDAVLNETKLNAGTQVEATLE
                     DKILFHNDSEMDLSDLRRRARALGGRFQLKASKSEYLVSNNPSRLEADDILLSPGTSG
                     DVLLKIFCDYDKRVGQLQVLEADRPIMVGEVPVRSTSTLHDGDTIRIDIGQVLRCNFS
                     ERILEEERNIIRTLEVKEVTHRFPGGETALDGISFNVTRGELVCVMGASGSGKSTLLR
                     VLAGQLQPTNGEVIVNGQSLYANLDTLKRYLSYIPQEDAFDEHLTIGENLQFAAAIRS
                     PHLSRRDRMRRLEGKLIELGLGERRDAIVGSVLKKTLSGGERKRLNIGLDMIGMSDVY
                     LFDEPTSGLSSKDSEHVLEIIRGMAHNKIIIVTIHQPSSKLFQMFHKAILLDKGGQLV
                     FFGTPNDMLRYFAEAEHQHQFGAELGACPSCGTTRPEFIFDVLETPLRDLSGDIIYEE
                     NAKGQLVPARRYSPEFWRDKYEAFRLIQDVKQVSMRKEPVAPLPPAPAQKKRLPFRWH
                     DEWTQFRTLLRRAFMSKLRNRANVVITVGVAPILALLIGTILRYSDSGKYDFASAYHI
                     PTFLFLALIVAMFLGLTNSADDIIRDRAILQRERNLDVRMPYYVISKTLTLGIFALIQ
                     SVLFVLIGNYILEIRGMFWLDLGIMFMTAMGGVSLGLIVSSLVADPKTAANIVPLVLI
                     PQIIMGGALIKYEDMNRNLALLYSLTHWFSEHPSQEQTKKMASKLEVPLICQFIAMRW
                     SYEEMIVAHAKLNPLTRRQDEANEQIQELAPKMKTAEEHKRLDDLKDVLAVLSGLQGH
                     FADEIDHYLKLTDPVLRGQQPFNRDLFKNATGPITAEQLYVNQKISDLISNAEMEQSD
                     YRRGTRPNVFFGAQKHYLGLHVSVFAFNTIVLIASTLALLILLDWILRRQLEVRRT"
     gene            5113..5505
                     /locus_tag="VFO30_07070"
     CDS             5113..5505
                     /locus_tag="VFO30_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9419085.1"
                     /translation="MAKPPFTLGVVGHFGLAVRDPKKSARWWQRVIGLRKEFEFENGV
                     AVGNDNVTIALFKGKPSPETIDHMSFHLPNMSILREALAHLKRHKVDMEDPGDEIGPE
                     APGSPHMGLWFHDPDGYRWELSVQGGAK"
     gene            5506..>5724
                     /locus_tag="VFO30_07075"
     CDS             5506..>5724
                     /locus_tag="VFO30_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419086.1"
                     /translation="MYSVEADKSKRLIVISASGHVAKDQLKAAAAKVALLVKDFAPGF
                     SALTDFRWMESMDSSGAPHIAEIMDTLAQ"
BASE COUNT         1195 a         1622 c         1754 g         1153 t
ORIGIN      
        1 cgcgcttctt ccaattcggc gagcgtgatt ccttttaatc cgcgacgcgc ggcgagcagc
       61 gctgcttcgt tgatgacgtt ggccagctcg gccccggaat aaccgggcgt gccgcgcgcg
      121 accacggcaa gatcgacacc ttcggcgagt ttcactttct tggagtgcac gcgcagaatt
      181 tcttcgcggc ctttcacgtc ggggagattg acggtgattt gtcgatcaaa gcggcccggg
      241 cggagcaacg ccggatcgag cacatcgggg cgattggttg cggcgataat gatgacgcct
      301 tcctgcgtgt cgaagccgtc catttcgacg agcaaggcat tgagcgtttg ctcgcgctcg
      361 tcatggccgc cgccgacacc gtgaccgcgg tgacgaccga cggcatcgat ctcgtcgatg
      421 aaaatgatgc acggcgccga tttcttgccc tgctcgaaca tatcgcgcac ccggcttgcg
      481 ccgacaccga caaacatctc gacgaagtcg gagccgctga tggagaagaa tggaacatcg
      541 gcttccccgg caatggcgcg ggcgagcaag gttttgccgg taccgggcgg tccgactaac
      601 agcacacctt ttggaatgcg gccgccgagt ttctggaact ttttcgggtc gcgcaaaaac
      661 tcgaccagct cctgcacttc gtcctttgct tcttcgacgc cggcaacgtc gcggaaggtg
      721 atcttgtttt tgtcacgggc gagcatgcgc gctttcgatt tgccgaagtt gagcgcgcct
      781 ttgccggcca tgcggatttg ctggcggaac aggaaataga gaacgagcag gaagagcgcg
      841 atcggcagaa aaccggccac ggtctgggcg attacgttcg actcggtctt gatcgccggc
      901 tgaatgccgg cggcgttgag tttgtcctgc aggttgttgg tgaaattgag atagatcgtg
      961 gtgcggaact tgactggctg ggccggctgc gagccgacgc tttgccgcag gtaataaccg
     1021 ctcaaggcct gggtggcgcg gccttcctcg acgatgagcg tgagcgggaa attcttgtcg
     1081 ttaacgatct gtttgttctc gagcaattca aggaaccggt tgtaagggac ttcttcgacc
     1141 ggctgcatgc cgccgcggta aaagagcatg gccaacccga tgaacgcgaa ggcgatgacg
     1201 atgaggatga cgccgcgcca gttgaaattc ggctcgttgc cgcgggactt gttttcccgg
     1261 ttagaattac ggtcggatga ggccatgagg ggcgctgact attcggaagc ggaggttacc
     1321 atggtcgctt tagcaatgca aaagagcagg aggtgggccg aggaccagaa gtggatcagt
     1381 gaacggtgat cagccatcat aagatcgaca attgcgacgg ttggcgcgtt gaaagctttg
     1441 cccgttacgg gtaacgtgcc cctcgtaaaa acctagatga cggaattggt ctcaacagtc
     1501 gcggacaaga tcgaccgcgc ggtgcagaag gttacgccgt tcgcgtaccg ctttcggcgg
     1561 cgcgggcggc gcttgatgcg gcgggcgttg cgcaagccac ggagcgtatc ggagggaccg
     1621 aaccttctgc cgttgctcat ccaggtgctg gccagtttca cgaaagcgga cggcgttctg
     1681 ctcgaagaag agatcgactc ctcgttaggc tttctgcgtt atgattatcc ggaagcggtc
     1741 tactcggagc tgcgtgagct gttccggcaa gcactttacg agcagcagga cctggcggcg
     1801 atggcgcaaa agctcggcgc gcaattgagc accgaccgca agatcatgct cggcgtgcag
     1861 ctctatgacc tgatctcgca ggccgggctg aagcaggaac aggtcgtgca gttttactcg
     1921 ttcatgtcgc agctcggcat ggcggcgcag gcgattgaca tcgtttatca gttgaacgcg
     1981 tcggaggatt ccgatccggc gatttaccag cgcggcgcgt cgccgctgga atcgctctcc
     2041 ttcgccgcga atggcaaagc cgacgtgacg ctacggacgc tcgcgggcaa cgaccgactg
     2101 ctcgcgttcc gttatcacga tttgattctg ctgaagaatt acagcacaca gaacgtctcg
     2161 gtccgcgggc gaccactcgt gcgcgggggg ttctgccgaa tttatcccgg tcaacggatt
     2221 ctcgtcggcg accaagtgct gacttatcag gatctggcga cgtatttcaa cgcgaagaag
     2281 aacgtgtcga tgccgcacgt gttcgtgcgc gtaaacaagg acgcggacga agtggcgctg
     2341 gagcgggcgc gcagccgtga gagcgcgctt gagatcacgt ttggcttgaa agtgcgggtg
     2401 aaagcgttgc gcgacgtcga cgcggtgttg aacgagacga agcttaacgc cggtacgcag
     2461 gtagaggcga cgctcgagga caagatcctt ttccacaacg acagcgagat ggacctgagc
     2521 gacctgcgcc ggcgcgcgcg tgctctcggt ggccggtttc agctcaaagc gtcgaagtcg
     2581 gaatatctgg tgtcgaacaa tccgagccgg cttgaagcgg acgacattct actgtcgccg
     2641 ggcacaagcg gcgatgtgct cctgaaaatc ttttgtgatt acgataagcg cgtcgggcaa
     2701 ctgcaagtgc tcgaagccga ccggccgatc atggttggcg aggtgccggt aagatcgaca
     2761 tctacgttgc acgacggcga caccatccgg atcgacatcg gtcaggtgct gcggtgcaat
     2821 ttttctgagc gcatccttga agaagagcgt aacatcatcc gcacgctcga agtcaaggag
     2881 gtgacgcacc ggttccccgg cggcgagacg gcgctcgatg gcatctcgtt caacgtcacg
     2941 cgtggcgagc tggtgtgtgt gatgggcgca agcggctcag gcaaaagcac attgctccgc
     3001 gtgttggccg ggcaacttca accgaccaac ggtgaagtca tcgtgaacgg gcagtcgctc
     3061 tatgcaaatc tcgacacgct caagcgctac ctcagttata tcccgcagga agacgcgttc
     3121 gacgagcatc tgacgatagg cgaaaacctc cagttcgcgg cggcgattcg ctcgccgcat
     3181 ttgtcccgac gcgaccggat gcggcggttg gaaggcaaac tgatcgagct cggcctcggc
     3241 gaacggcgcg acgcgatcgt cggcagcgtg ttgaaaaaga ccttgagcgg cggcgaacgg
     3301 aaacgcctca acattgggct cgatatgatc ggcatgtcgg acgtttacct gttcgacgag
     3361 ccgacctccg gcctctcgtc gaaagattcc gagcacgtcc tggagatcat ccgcgggatg
     3421 gcgcacaaca agatcatcat cgtcacgatc catcagccga gctcgaagct gttccagatg
     3481 tttcacaagg cgatcctgct cgacaagggc gggcagctcg tctttttcgg cacgcctaac
     3541 gacatgttgc gctatttcgc cgaagccgaa catcaacatc agttcggcgc cgagcttggc
     3601 gcgtgtccgt cgtgcggtac gacgcgaccg gaatttattt tcgacgtgct cgagacgccg
     3661 ctgcgcgacc tgagcggcga catcatttac gaggagaacg ccaagggcca gctcgtcccg
     3721 gcgcggcggt attcgcctga attctggcgc gacaaatacg aagccttccg cctgatccag
     3781 gacgtcaaac aagtttcgat gcgcaaagaa ccggtcgcac cattgccgcc agcgccggcg
     3841 cagaaaaaac ggctgccgtt tcgctggcac gacgagtgga cgcagttccg cacgctgttg
     3901 cgccgcgcgt tcatgagcaa gctgcgcaac cgcgcgaacg tcgttatcac cgtcggcgtc
     3961 gcgccgatcc tggcattgct cattggcacg attttgcgct attccgacag cgggaaatac
     4021 gatttcgcat cggcctacca tatcccgacg tttctgttcc tggcgctgat tgtcgcgatg
     4081 ttcctcggcc taacgaacag cgcggacgac atcatccgcg accgcgccat cttgcaacgc
     4141 gagcgcaatc tcgacgtgcg gatgccctat tacgtgatct cgaaaacgct cacgctgggg
     4201 attttcgcgc tgattcaatc ggtcctcttc gtgttgatcg gcaactacat cctcgagatc
     4261 cgcgggatgt tttggctcga tcttgggatc atgttcatga cagcgatggg cggcgtgtcg
     4321 ctcgggctga ttgtttcctc gctcgtggcg gacccgaaaa cggcggccaa catcgtcccg
     4381 ctggtgctga ttccgcagat catcatgggc ggcgcgctca tcaagtatga ggacatgaat
     4441 cgcaacctgg cgttgctcta ttcgctgacc cactggttta gcgagcaccc gagccaggag
     4501 cagacgaaaa aaatggcgag caaactggag gtgccgttga tctgtcagtt catcgccatg
     4561 cgctggtcct acgaagagat gatcgtcgcg cacgccaagc tcaacccact aacgcgccgc
     4621 caggatgagg caaacgagca gattcaagag ctggcgccaa agatgaagac ggccgaggaa
     4681 cacaagcggc tggacgattt gaaggacgtg ttggccgtgc tttcaggcct gcagggacat
     4741 ttcgctgacg agatcgatca ttacctcaaa ctcaccgacc cggtgctgcg tggtcaacag
     4801 ccgtttaacc gcgacctgtt caaaaacgct actggtccga tcaccgccga acagctctac
     4861 gtcaaccaga agatttccga cctcatctca aacgcggaga tggagcagag cgactatcgt
     4921 cgcggcacgc ggccgaacgt tttcttcggc gcgcagaagc attacctcgg actgcacgtc
     4981 agcgtctttg cctttaacac aatcgtgctg atcgcctcca cgctcgcctt gctgatcttg
     5041 ctggactgga tcttgcggcg acagctggaa gttcgcagaa cgtagccgtc gcgccggcgc
     5101 aagatcgaca atatggccaa gcctccattt acgctgggcg tggtgggcca ctttggactc
     5161 gcggtgcgtg atccgaagaa gagcgcacgg tggtggcagc gcgtaatcgg gttgcgcaaa
     5221 gaattcgagt tcgaaaacgg cgtcgcggtc ggcaacgaca acgtcacgat cgcgctcttc
     5281 aaaggcaaac cttcaccgga aacgatcgac cacatgtcgt ttcatctgcc gaacatgtcg
     5341 atcttgcgcg aagcgctggc gcatttgaaa aggcacaaag tcgacatgga agatcccggc
     5401 gacgagattg gcccggaggc gccgggttcg ccacacatgg gcctctggtt tcacgatccc
     5461 gatggctatc gttgggaact tagtgtccag ggcggcgcca agtaaatgta ttcagtcgaa
     5521 gccgataaat caaaacgcct gattgtcatc agcgcgagcg gtcacgtggc aaaagaccaa
     5581 ctgaaagcag cggccgctaa ggtcgcgctg ttggtaaagg atttcgcgcc gggcttctcc
     5641 gcactcaccg atttccgctg gatggaatcg atggactcat ccggcgcgcc gcacatcgcc
     5701 gagatcatgg acacgcttgc gcag
//
LOCUS       DASTDG010000214         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1331596, whole genome shotgun sequence.
ACCESSION   DASTDG010000214 DASTDG010000000
VERSION     DASTDG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331596"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..2417)
                     /gene="pheT"
                     /locus_tag="VFO30_07080"
     CDS             complement(81..2417)
                     /gene="pheT"
                     /locus_tag="VFO30_07080"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9419087.1"
                     /translation="MKFSVNWLREFVDVPDSADEVAELLTRAGVETKNIEHRGANIDK
                     VIVAQITASSRHPNADRLTVCEVVDASGTTRQIVCGATNYKVGDKVPLALPGAKLPNG
                     LEIRKSELRGVESEGMLCSAKELGLGDDASGLLILSPEAKIGVAIADVFPNDTILDVE
                     TTPNRGDLLSHYGLAREIAALSGKKLRASLPPRTAAATESGVTIAAGYECPFFSIRKI
                     DKVKVGSSPEWLRAKIESVGIRSINNIVDISNYVMLELGQPTHAFDAGRMRGGINVRL
                     ARGGEKFLALDGKTYSLGRNHLVVADEERAVGIAGVMGGDETGVTESTQNILLESAYF
                     LPASIRRAARELNLPSDASYRFERGVDPEMVLRASERATELIREIAGGTPAKEVRVAG
                     ELPENPADVSLSYSKCDQLVGIQIEPKQVDEILKRFGLCRSKSRRTSGRSTWSIPSYR
                     RDLQRDVDLIEEVIRAYGVERIIGTDRSRFTAATPADREYDWEARLRERLATHGLSEA
                     RTSKLIPRYAPAFSDNAVDLRNPLSEDHVALRPSLISSLLGVLERNIRAGAEKVVIFE
                     LGRAFIPPSGKEERHLAILLWGDLSTQSDWRSPNKRPLDLFDLKGALNSVVPELSFRK
                     ATYPDLAPAAEVCCADRMIGFAGQLSATKTIHAGAILIAELHADLLFNTREAATKFRE
                     LDRFPTITRDIAMIVPEMTSHAEVLRVIENPKEPLLATVELFDLFSGSEVGEGRKSLA
                     YRLTYRHAGRTLTHEEVTATHAKIRERLRRELGAELRE"
     gene            complement(2551..3567)
                     /gene="pheS"
                     /locus_tag="VFO30_07085"
     CDS             complement(2551..3567)
                     /gene="pheS"
                     /locus_tag="VFO30_07085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9419088.1"
                     /translation="MLHPLEHLRADALREIESAPNEQALEAARVKYLGKTGSISAWGE
                     QLKSLPAEEKPAIGKLLNDVRTTVSGALQRRAETFRADKESDALAKIDISLPGTPHER
                     GSLHPLTQMLDRSIAIFRRMGFALAEGPDIEDEWHCFDALNTPPDHPARNEQDTFYLP
                     DGRLLRTHTSTVQIRAMQTAPPPIRVIAPGAAYRRDEVDATHSAQFHQIEGLYVDDNV
                     SIADLKGTLEFFMRELFGSDTAVRFLPHYFPFTEPSFEVYVRSKALKSGEQWIEVAGC
                     GMVHPAVFEQVNTERGDDAYDPDKWSGFAFGLGMDRLAMILFDIPDIRLFAQNDLRFL
                     KQFA"
     gene            complement(3648..4034)
                     /gene="rplT"
                     /locus_tag="VFO30_07090"
     CDS             complement(3648..4034)
                     /gene="rplT"
                     /locus_tag="VFO30_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9419089.1"
                     /translation="MPRATNAPASRARRKRVIAKAKGYRGRRSKLFRYAKDATMKGQY
                     WAYRDRKTRKRTFRYLWIQRLNAAARASGLTYSRFAEGLKAAGIGLDRKILADLAVTD
                     EPAFKSIVDQAKTALADKAKSKDKAA"
BASE COUNT          795 a         1232 c         1222 g          907 t
ORIGIN      
        1 aagccgtcgg tcgagagttg agcggaaagc actggtttcc ttcccaaccc tcaaccaacg
       61 gctctcactt ttcgaaagaa ctattcgcgc aattccgcgc cgagttcacg ccgcaaacgc
      121 tcacgaatct tcgcatgcgt cgcggtcact tcctcgtgtg tcagtgtgcg acccgcgtgc
      181 cgatatgtca atctataagc gagcgacttg cgtccctcac ccacttcgct gccgctgaac
      241 agatcgaaca actcgacggt ggccaacaat ggttcctttg gattttcaat cacgcgcagg
      301 acctccgcat gcgaagtcat ttccggaaca atcatcgcga tgtcgcgcgt gatggttggg
      361 aaacggtcga gctcgcgaaa ctttgtcgcg gcttcgcgcg tgttaaaaag aagatcggcg
      421 tggagttcag cgatgagaat tgcgccagcg tggatcgttt tcgtcgccga aagttgtccg
      481 gcaaacccaa tcattcggtc ggcgcagcaa acttctgcgg cgggcgcgag gtccggataa
      541 gttgccttgc gaaaagacag ttccgggaca accgaattca gcgcgccctt gagatcgaac
      601 aaatcgagag gccgcttgtt cggagatcgc caatccgatt gggtcgataa gtcgccccag
      661 agcagaatgg caaggtgccg ttcttctttt ccggacggcg gaatgaacgc gcggccgagt
      721 tcaaagatga caaccttctc cgctccggct cgaatgtttc gttcgagcac tccgagcaag
      781 ctgctgatca agctcggccg gagcgcgaca tggtcttcgc tcaacgggtt gcgcagatca
      841 acggcattgt cgctgaaagc cggagcgtac ctcggaatca atttcgacgt gcgcgcttcc
      901 gaaagtccgt gtgtcgccag gcgctcacgc agccgcgctt cccaatcgta ctcgcggtcg
      961 gcgggggttg ctgccgtgaa ccggctccgg tccgttccga tgatcctctc gacgccatag
     1021 gcgcggatca cttcctcgat tagatcgaca tcccgctgca aatcgcgtcg ataactcgga
     1081 atcgaccatg tcgatcttcc actcgtgcgc ctgctcttgc tcctacacag cccgaaccgc
     1141 ttcaagattt catcgacctg tttcggctcg atctgaatgc cgactagctg gtcgcatttc
     1201 gaatagctca gcgagacatc tgccggattt tcaggcagct cgcccgcgac gcgaacttct
     1261 ttggccggag ttcctcccgc gatttcgcga atcagctcag tcgcgcgttc cgaagcgcgc
     1321 aacaccattt cgggatcgac accgcgctcg aaacggtagc tcgcgtcgct tggtagattc
     1381 aattcgcgcg ccgctcggcg gatactcgcc ggcaaaaagt acgcgctctc gagcaaaatg
     1441 ttctgggtcg actcggtgac gccggtctcg tcgccgccca ttactccggc gatgccgacg
     1501 gcgcgctctt catccgcgac gacgagatga tttcggccta acgagtaggt cttgccgtcg
     1561 agcgcgagaa acttctcgcc gccgcgcgcg agacgcacgt tgatgccgcc gcgcatcctg
     1621 ccggcatcga acgcatgcgt cggctgtcca agctcaagca tcacgtaatt cgagatatcg
     1681 acgatgttgt taatcgagcg aatcccgacg ctctcgattt ttgcgcgtaa ccactcggga
     1741 ctggacccaa ctttgacctt gtcgatcttg cgtatagaga aaaacggaca ctcatagcct
     1801 gccgcgatgg tgacgccgct ttctgtagcg gccgccgtcc tcggcggcag agatgcacgc
     1861 aatttctttc cggagagcgc cgcgatctcg cgagcgagtc cgtaatggct caacaaatcg
     1921 ccccggtttg gcgtggtttc gacgtccaga atcgtgtcgt tcggaaaaac atcggcaatc
     1981 gccacgccga tctttgcttc cggcgacaaa atcagaagcc cggaagcatc atcgccgagt
     2041 ccgagctctt tcgcgctgca aagcattcct tcgctctcga cgccccgcag ttcgcttttg
     2101 cgaatctcca gtccattcgg cagcttcgcg ccgggcagcg cgagcggtac tttgtcgccg
     2161 accttgtaat tggtcgcgcc gcaaacgatc tggcgcgtcg tgccgctggc atcaaccact
     2221 tcgcagaccg tgagccggtc cgcgttcggg tgccgcgacg aggcggtgat ttgcgcgacg
     2281 atgactttgt cgatgttcgc gccgcggtgc tcgatgtttt ttgtttccac gccggcgcgc
     2341 gtcagcaact cagcgacctc atcggcactg tcgggaacat cgacaaattc gcgaagccag
     2401 ttgacggaga acttcatgtg gagatggtag cgcgcgcgtc tcgcgcgcgg cgctcggtgt
     2461 cccgccgagc gcaacttact gaagatgttc gccgcgagac gcggcgaacc gcggacgaga
     2521 cgcccgcgct acccttctgg tgcgagtggc tcatgcgaat tgtttcagaa agcgcagatc
     2581 gttttgtgcg aaaaggcgaa tgtcgggaat gtcgaacaaa atcattgcca gccgatccat
     2641 accgaggccg aacgcgaaac cgctccactt gtccgggtcg taggcgtcgt ccccgcgctc
     2701 cgtgttcact tgctcaaata cagctggatg caccatgccg cagccggcga cttcaatcca
     2761 ttgttcgccg ctcttgagcg ctttcgacct cacataaact tcgaagctcg gctcggtgaa
     2821 aggaaaatag tgcggcaaaa aacgcacggc cgtgtcgctg ccgaaaagtt cgcgcatgaa
     2881 aaattccagc gtgcccttca aatcagcaat actcacgttg tcatcgacat agagaccttc
     2941 gatctggtga aactgggcgc tgtgcgtcgc gtccacttcg tcgcggcgat atgctgcgcc
     3001 gggggcgatc acgcggatgg gcggaggggc agtttgcatc gcgcggatct ggacggtcga
     3061 ggtgtgggtg cggagcaatc gaccgtcggg cagatagaat gtgtcctgct cgttgcgcgc
     3121 ggggtggtca ggcggcgtgt tcagtgcgtc gaagcaatgc cattcgtctt cgatatcggg
     3181 cccctcggcg agcgcgaagc ccatgcgtcg gaatattgcg attgatcgat cgagcatttg
     3241 cgtaagcgga tgcagcgagc cgcgctcgtg cggcgtgccc ggaagtgaga tgtcgatttt
     3301 cgcaagcgca tcggattctt tgtcggcgcg aaatgtttcg gctcgccgtt ggagggcgcc
     3361 actgacagta gtccggacat cgttgagcaa tttgccgatg gccggtttct cctctgccgg
     3421 gagcgatttc agctgctcgc cccaggcgga gatgctgccg gttttgccga ggtactttac
     3481 gcgcgcggct tcgagcgcct gctcgttagg cgcggactcg atctcgcgca gtgcgtcggc
     3541 gcggaggtgc tcgagcggat gaagcatggc ggaatcgtga atcgttaaat cgttacatcg
     3601 ttaaatcgga aaatcgattc acgattcacg aattacgatt caacgaatca cgccgccttg
     3661 tctttcgact tcgccttgtc ggcgagggcg gttttcgctt gatcgacaat cgacttgaat
     3721 gccggttcgt cagtgacggc caggtcggcc agaatcttgc gatcaagtcc gatgccggcg
     3781 gctttaagac cttccgcgaa acgggaataa gtaaggccgc tcgcgcgcgc ggcggcgttg
     3841 aggcgctgga tccagagata acggaaggtg cgcttccgcg tcttgcggtc gcggtaggcc
     3901 cagtactggc ctttcatggt cgcgtctttg gcatagcgga acagtttcga gcggcgaccg
     3961 cgataacctt tcgcctttgc gatgacgcgt ttgcgccgtg cgcgactggc cggtgcatta
     4021 gtagctctgg gcatgttttc ctgccccgcg ttggcgaggc ctcctctttc agcgagtcgt
     4081 taattgtcgt tgtcgatctt ccaaccccac tcgctgattg gtccccgatg caggacaggc
     4141 ggaagatcgt taccgg
//
LOCUS       DASTDG010000215         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1209576, whole genome shotgun sequence.
ACCESSION   DASTDG010000215 DASTDG010000000
VERSION     DASTDG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209576"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFO30_07095"
     CDS             <1..484
                     /locus_tag="VFO30_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419090.1"
                     /translation="RDAAIEKFARVLLRRYGVVFRRLLERESLDVSWYELGRVYRRLE
                     ARGEIRGGYFVGGVSGEQFALPEAIGLLRSIRKAAPGEELVTLSAADPLNLLGILTAG
                     QRVAAFTANRILFRGGIPAAALEAGEIRKLSDDSVSDSEIERALKVGKLRPSLRPYYK
                     "
     gene            complement(624..1034)
                     /locus_tag="VFO30_07100"
     CDS             complement(624..1034)
                     /locus_tag="VFO30_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419091.1"
                     /translation="MFTSSNKPTVLKIAVAATFIAALAVSPLAVAQEHEHGKQDLTAA
                     AEKTVTGEVVDLMCYIDHGATGDKHAACGNKCIKGGGPVGVVQNGKAYLVVGEHKPIN
                     DELADSCGKTVTIKGKVAERGGMVMLENAEVVKK"
     gene            complement(1103..2245)
                     /gene="carA"
                     /locus_tag="VFO30_07105"
     CDS             complement(1103..2245)
                     /gene="carA"
                     /locus_tag="VFO30_07105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9419092.1"
                     /translation="MRAILALEDGRYFEGESFGATGTRVGEICFNTAMTGYQEVLTDP
                     SYRGQIVAMTYPLIGNYGINSLDQESQSPHVRGFVIEELSEAPSNWRAEMSLDEYLRK
                     WEIPGVQGIDTRALTRHLRTRGAMKACLTTEKMSIEEAVQRAVDGEGVVGMDYVREVS
                     TPAVYQWDPEDKLSQPWTIVNGNGDNKPKLPPVHFRIVAYDYGIKQNILRLLRQKGFS
                     VTVVPATTTADEVLALNPDGIFLSNGPGDPAALPYAHKAVHDLLGKKPIFGICLGHQI
                     LGFAFGGSTFKLKFGHRGANQPVKDLQSGKVAITAQNHGFAVDPNTLPRQVEVTHVNL
                     NDGTVEGMRHKDVPIFSVQYHPEAAPGPHDASYFFSQFADLIEKRK"
BASE COUNT          528 a          782 c          799 g          636 t
ORIGIN      
        1 ccgcgatgcg gcgatcgaga agttcgcgcg cgtgttgttg cgccgttatg gcgtggtgtt
       61 tcgccgtttg ctggagcgcg agagtttgga tgtgagttgg tacgagctcg gccgggttta
      121 tcgtcgcctg gaagcgcgcg gtgaaatccg cggcgggtat tttgtcggcg gcgtcagtgg
      181 cgaacaattc gccttgccag aagcgattgg tttgctgcgc tccattcgca aagctgcgcc
      241 gggcgaggaa ctggtcacac tcagcgcggc ggacccgctc aatttgttag gcattttgac
      301 ggccggccag cgggtcgcgg cgttcacggc caatcggatt ttgttccgcg gcggaattcc
      361 ggccgcggca ctggaagccg gcgagattcg gaaactgtcg gatgattctg tttccgattc
      421 agagatcgaa agggcgctca aagtcggaaa gctgcggcct tcactgcggc cgtactacaa
      481 gtaaccgcgc gccaggcgcg gtcgtccaga gccgcggaga cggcgaggga cctcaccaac
      541 tcgctgttga gcacacaatg taggcagcgt gatcgagacc agcttttgtg aggtccttcg
      601 ctccgctcag gatgacaggt ggattatttc ttaacaacct cggcgttctc gagcatgacc
      661 atgccgccgc gctcggcgac tttgcccttg atcgtgacgg ttttgccgca cgaatcagcc
      721 agctcatcat tgatcggttt gtgttcgcca acgacgagat aggccttgcc gttctgaacc
      781 acgccgaccg ggccgccgcc cttgatacac ttgttgccgc aggcggcatg tttgtcgcca
      841 gttgcgccgt gatcgatgta gcacatcagg tcaaccactt cgccggtgac ggttttttcc
      901 gctgccgcgg tcagatcctg ttttccgtgc tcgtgctcct gcgcaacggc cagcggagaa
      961 actgccagtg ccgcgatgaa cgtcgcagcg acagcgattt tcaagacagt cggtttattt
     1021 gatgacgtaa acataagcga atctctgccg attctcggag tggtcaattg tagctgccgc
     1081 cgtctctggc ggcagatgac ggttattttc gcttctcaat caggtcggcg aattgggaaa
     1141 agaaatacga cgcatcgtgc ggaccgggcg cggcttcggg atgatactgc acgctgaaga
     1201 tcggcacatc cttgtgccgc atgccctcga cggtgccgtc gttcaaattc acatgcgtca
     1261 cctcgacttg acgcggtaac gtgttcggat cgacggcgaa gccgtggttt tgcgccgtga
     1321 ttgcgacttt accgctttgc agatctttga ccggctggtt cgcgccgcga tggccgaatt
     1381 tcaatttgaa tgtcgatccg ccgaaggcga agccgaggat ttgatggccg agacaaatgc
     1441 cgaagatcgg ctttttgcct aacaaatcgt gcactgcttt atgcgcgtag ggtagcgccg
     1501 ccgggtcacc cgggccgttc gacagaaaga tgccgtcggg attgagcgcg agcacttcgt
     1561 cagcggttgt cgtggcgggc acgaccgtaa cgctgaagcc tttctggcga agcaaccgca
     1621 gaatattctg cttaatcccg taatcgtaag cgacgatgcg aaagtgcacc ggcggaagct
     1681 tgggtttgtt gtcgccgttt ccgttcacta tcgtccacgg ctgactcaac ttgtcctcgg
     1741 gatcccactg gtaaactgcc ggggtcgaaa cttcgcggac ataatccatc ccgacgacac
     1801 cttctccgtc gaccgcgcgc tgaaccgctt cttcgatcga catcttctca gtcgttaggc
     1861 aagctttcat cgcgccgcgc gtgcgcaggt gccgcgtgag tgcacgcgtg tcgattccct
     1921 gcacaccggg gatctcccat ttgcgcaagt actcatcgag cgacatctcg gcgcgccagt
     1981 tgctcggcgc ttcgctcaat tcttcgatga cgaatccgcg cacatgcggc gattggcttt
     2041 cctggtcgag cgagttgata ccgtaattgc cgatgagcgg atacgtcatc gccacgattt
     2101 ggccgcggta tgagggatcg gtcagcacct cctgataacc ggtcatcgcc gtgttgaagc
     2161 aaatctcgcc gacgcgcgtg ccggtcgcgc caaatgactc gccttcgaag tatcttccgt
     2221 cttcgagggc gaggattgcg cgcatgctgg aacggattgt cgatcttgtc tcgacatttt
     2281 caaactcaga cgcgggtgcc caccgttccg agatcgtgga tcgagattcg agattcggag
     2341 ttcgctgatg taacgaatca acgatttaac gaatcacgaa aaaacccgga tcgaggattc
     2401 gatccgggtt ttttaaatct taccccgctg gtgccgttta ggcggatcac ccgttccctt
     2461 tcggtaaacg gtggacgacc gtcgcgacag attctgactt ccaatcaagg catgtcatct
     2521 ccgacgcaga cccagcctcc tgattcgtca actatcggtt cggggatgtc gcctggtatc
     2581 tcgaacatcg cagggtaaat ttgaaagccg tcggtcgaga gttgagcgga aagcactggt
     2641 ttccttccca accctcaacc aacggctctc acttttcgaa agaactattc gcgcaattcc
     2701 gcgccgagtt cacgccgcaa acgctcacga atcttcgcat gcgtc
//
LOCUS       DASTDG010000216        10363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1326217, whole genome shotgun sequence.
ACCESSION   DASTDG010000216 DASTDG010000000
VERSION     DASTDG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326217"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFO30_07110"
     CDS             <1..591
                     /locus_tag="VFO30_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419093.1"
                     /translation="FSPERQTRVEIGSDNLIREHCTIHRGSADGTATKIGSNNFLMAG
                     AHVGHNCEIENNVIIANNCLLGGYVRVDDGAFLGGGCVFHQHMHVGRLAIAQGGSAFS
                     KDIPPFVVAAERNYVFGLNVVGLRRAGFSAQDRTEIKAAFKLLYTSGFNISQALEEAA
                     TMNFGSAGREFFEFVANARKRGICPLKRGGDEEMSI"
     gene            complement(624..1280)
                     /locus_tag="VFO30_07115"
     CDS             complement(624..1280)
                     /locus_tag="VFO30_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9419094.1"
                     /translation="MEPTIDHFPALDRIGICRSAFITRIPGIDVSHDKLKALEHLEAA
                     HRKFRRELGFGTVPLATAEQIHGDRITLVDLAIGSDTELAGCDGLITNRHDVVLGIHV
                     ADCCAVYIVDRKTPAIGLVHSGKKGTALEIVIKAISEMRHCFGSDPAQLVVQLSPCIR
                     PPHYEVDFAAAIIEQCRAAGVQQIHDSGVCTACDLDRYYSYRAEKGKTGRMLALLGLL
                     "
     gene            complement(1322..2863)
                     /locus_tag="VFO30_07120"
     CDS             complement(1322..2863)
                     /locus_tag="VFO30_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9419095.1"
                     /translation="MRKTFCVLSLFVGVTAASPLSLGGVSTGGARGSGTQGSHTDPPT
                     FTLDRAILTALKQNPSILNAKQEIERSKGVILQIGAEALPRVAPTANLNWTDPALGSS
                     GFAASGGVGGGVPGGTDTSYAIRITGTQLFFNYSTFRSIRGTFFQRDNAYFGFRDIVD
                     QVIATTKTQFYQIILNKALIGVQEESVRLLESQLKDQQNRFEAGTVPRFNVLQAQVAL
                     SNQIPNLIAARNNYRISQLQLAKTLGLDFIPRRGENPPLEAEGEMQYIAQPLGLADAI
                     ELGKQRRPFLKRQRAAVLNQIEQVHVALGGFLPNFNTTGGYDVFSSPLTTSWDEIRQG
                     WVFGLVGNWAVWDSGATYGNYKQQRALLSEAEITYDDDVRQVELEVQQAYSNLQQNRE
                     LIQATEKTVEQATEALRLAKARLDAGAGTQLDVLNAQVQLTQAESTRLQALFGYDSSL
                     AEFNRVTGAQATYTEAFSGIAPRATQSRTYYTGSDVDARGKPKRRTGLESIPVTPMSS
                     QPSGK"
     gene            complement(2966..3442)
                     /locus_tag="VFO30_07125"
     CDS             complement(2966..3442)
                     /locus_tag="VFO30_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9419096.1"
                     /translation="MSTLAASPRAREENEQIRADAERLSHIVLEMQRCFVLHLCKELA
                     PGNVSFPQFFLLAYLDQKEVLTMSAIAQKMGHTTAAASGLVARLENLGYVVRSVARED
                     RRKVMVCITPKGSALVRRIREEMVGNLMKLMGHLTPDEQKSWLHIYSKIYDYCQSK"
     gene            complement(3505..4680)
                     /gene="glp"
                     /locus_tag="VFO30_07130"
     CDS             complement(3505..4680)
                     /gene="glp"
                     /locus_tag="VFO30_07130"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET9419097.1"
                     /translation="MTSEEKARATILDSVRPLSTRRLSILRGLDCFAGQDYFARVPLP
                     LFDNSAMDGYAVMASDCAPGSRLRVIAEQPAGFDRHLRVAAGEAVRIFTGAPLPQGAD
                     AVVMQEDVTADGNEVVVNVHITSGEFVRRRGCDLGEGQKVIRGGERVRAVTLGLLASQ
                     GLAEIVVGGEVQAAIISTGDELARPGDKLEAGQIYESNSVLLGSLLQRCGAKLMLTQH
                     CTDDATALREAFHAATKYQVFIVSGGVSVGKHDLVQQSLRDLGAQIDIWRVAIKPGKP
                     FLFGRLENCLVFGLPGNPVSAFITFLKFVRPAILKLMGAGDLELPTTRATLVVDLANE
                     SDRVHYIRGKLEHGNFAPVGRQESHALFGLSQSNALLRLPVAAAVARGATVDLEIWD"
     gene            4805..4895
                     /locus_tag="VFO30_07135"
     tRNA            4805..4895
                     /locus_tag="VFO30_07135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4839..4841,aa:Ser,seq:gct)
     gene            complement(5016..7046)
                     /locus_tag="VFO30_07140"
     CDS             complement(5016..7046)
                     /locus_tag="VFO30_07140"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET9419098.1"
                     /translation="MVPPKRFSTVSAIAALTALYFVAGKLGLHLAFLHRSASPVWPPA
                     GIALAALLVLGYRMWPAIFIGAFLVNATTAGNVLTSLGIASGNTLEAVCGAWLVNHFA
                     GGTKVFERARGVFQFAIAAMLSTLLSPSIGVTTLALGDFASWTSYWPIWLTWWLGDAA
                     GDLIITPLILLWSIPSPRRWTRREAIEVSILLFLLVCLAEIVFGGWSSISARNYPISF
                     ICGPIVIWMAFRFSQRETATGIFILSAIAIWGTLHGAGPFVMDTENQSLLTLQTWTAV
                     LTITAMALSAAMAERRRAEAAIEQQKSAVETANRTKDNFLAMLSHELRTPLTPVITAL
                     DSLEIDSAKTEEAKAALAMIRRNVELENQLIGDLLDLTRIAKDKLELKFDTLDAHEAV
                     LNVIDICRAEANAKQLHLYSDLRAGAHHISGDSAKFQQIIWNLLKNAIKFTPENGEIN
                     ISSTNPAPQTFTISVRDTGIGIDPEIIDRVFDPFEQGELTLRRRFGGLGLGLAISKSL
                     AKAHGGTLVARSPGRDKGTTFLLTLNTVPPQQRPTASVAAPVPSRTLRILLVDDHHDT
                     CTALEKLLVRRGHLVAATHNVRAALEAAVRNQFDLLISDLALPDGTGMNLMMQVRAIC
                     GMPGIAISGFGTNGDIERSLQAGFAEHLTKPVNLERLEAAMKRVVGSSVAAR"
     gene            complement(7334..7738)
                     /locus_tag="VFO30_07145"
     CDS             complement(7334..7738)
                     /locus_tag="VFO30_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HET9419099.1"
                     /translation="MKTLLALAIAVAFVGTAAADIQDPPGNDYGPTRKLGRGLSNFFL
                     GSAEIFSTVGQINKDEGNNAAFGYGMVRGFGRSAVRHVAGFIEAVLAPFPTQHGTYYP
                     LLPSDIPWIHAGYSEFPPELGNETKYPYVRDY"
     gene            complement(7783..8733)
                     /locus_tag="VFO30_07150"
     CDS             complement(7783..8733)
                     /locus_tag="VFO30_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9419100.1"
                     /translation="MKVSRVPTSIWFWVAFHIGVFFALIVDLAQFKHRGRPLSMRAAA
                     QRTAIWILLSLGFNLIVWKIRGPDHALDFLTGYVIEYSLSVDNIFVFILIFTYFGVPP
                     IAQHRVLVWGIVGALVMRGVMIWLGLALVERFHFVVYLFGVFLIITAIRMLLGKTRDQ
                     DFGKSLVMRFCKTWLPITPEFHNADFKVRIADRWMLTPLALALIVIDVMDLVFAIDSI
                     PAVIAITQDPFIVYTSNICAILGLRSLYFLLARLIDRFVYLKTGLAIVLGFVGVKMTI
                     ADVVHVPTGISLLVIVLVLAVTIMMSMIATRNRAAAKRLK"
     gene            8816..>10363
                     /locus_tag="VFO30_07155"
     CDS             8816..>10363
                     /locus_tag="VFO30_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419101.1"
                     /translation="MDAELEKLVEAGKLTDKAAERLEQLKPGTFCLHKSWGFGRVAEW
                     NLLLNQIVIDFAAKKAHAMQLQYAADYLTPIPPEHFLARKADDLTGIKQLARDQPVAL
                     VRNILESLGGKATPQQIGEWMIGDIFSEPEWKRWWESTKKLLRKDGHFSIPAKKNEPI
                     ELRSAPVSRADELLARFTHARQPREQIAALDEIVKSHQEFNEPEKQLQPVIAAIENAA
                     ARNQKLHPELSFEFVLGRDDLSQRFPQLKTTHIGLTVGKLIEDEERRLMSILPKLAAA
                     KEKRILQTLPTALGERWSARALQLMQATHGRMVAQIPHVFRDAGQHLELQKMLERSIR
                     EHSATSEMLTWLCAERNEWRELITPELLSAILSALEREQHQAPGRASKLQRLLIEDRQ
                     LIADIFADTDVALARDAMRRLQLTPLFDELTKRSLLARLVKVYPELESMITGAKPEEK
                     AAPLVVSWSSLDKRKAEYEELVQRKIPENTKEISVARSYGDLSENFEFKAAKQMQAVL
                     MRRKTELE"
BASE COUNT         2277 a         3009 c         3024 g         2053 t
ORIGIN      
        1 ttttcgccgg agcgacagac gcgcgttgag atcggttcgg ataacctcat ccgcgagcat
       61 tgtaccattc atcgcggcag cgcggacggg accgccacga aaatcggcag caacaatttt
      121 ctcatggcgg gcgcgcacgt cggccataac tgtgaaattg aaaacaatgt cattatcgcc
      181 aacaactgtc tgctcggcgg ctatgtgcgc gtggacgatg gcgcgtttct cggcggcggc
      241 tgcgtgttcc atcaacacat gcacgttgga cggctcgcga ttgcgcaggg tggctcggcg
      301 ttcagcaaag acattccgcc cttcgttgtc gcagcggagc gcaactatgt ctttggattg
      361 aacgtggtcg gtctgcggcg tgccggcttc agcgcgcaag atcgcactga aattaaggct
      421 gcctttaaac tcctttacac gagcggattc aacatctcgc aggcgctgga agaagccgcg
      481 accatgaatt tcggcagcgc gggtcgcgaa ttcttcgaat ttgtcgcgaa cgcgaggaaa
      541 cgcgggattt gtcccttaaa gcgcggcggt gacgaggaga tgtcgatcta accagatttg
      601 tccggcgtct cacagagacg cgcctacagc aggccgagca acgcgagcat gcggccggtt
      661 tttccctttt ccgcgcggta ggagtagtag cggtcgaggt cgcaggccgt gcagacgccg
      721 ctgtcgtgaa tctgctgaac accggccgcg cggcattgct cgataatggc ggcggcaaaa
      781 tcgacttcgt aatgcggtgg acgaatacag ggactcagtt gcacgactag ctgagcgggg
      841 tcagatccaa agcagtggcg catttcgcta atcgctttga tcacgatctc caacgccgtg
      901 cctttctttc ccgagtggac gaggccaatc gccggcgttt tccgatccac aatgtaaacg
      961 gcacagcagt cggcgacgtg gatgccgagg acaacgtcgt gccggtttgt gatgagcccg
     1021 tcgcagccag cgagctccgt atcgctgcca atcgcaagat cgacaagtgt gatccgatcg
     1081 ccatgaattt gttccgcggt cgcgagcggc acagtgccga agccgagctc gcgccgaaat
     1141 tttcggtgcg cggcttcaag gtgctcgagc gccttgagct tgtcgtgcga gacgtcgatt
     1201 cccggtatcc gcgtgataaa cgcgcttctg caaataccga tgcggtcgag cgccggaaaa
     1261 tggtcgatgg tcggttccac tcccgaagct gcgcgcgccg tctgcgaact gacctgctcg
     1321 attatttacc gctcggctgg gatgacatcg gcgtaaccgg aatcgattcc aacccggtgc
     1381 gacgcttcgg tttgccgcgc gcatcgacgt cgctgcccgt gtagtaagtg cgcgattgcg
     1441 tcgcgcgcgg tgcgatgccg ctgaatgcct ccgtgtaggt ggcttgggcg ccggtgacgc
     1501 gattaaactc ggcgagggaa gagtcatagc cgaagagcgc ttgcaggcgg gtcgattccg
     1561 cctgggtcag ttgcacctgg gcattgagca cgtccaactg cgtcccagca cccgcatcga
     1621 ggcgcgcctt cgcgaggcgc aatgcttccg tcgcttgttc gaccgtcttt tccgttgcct
     1681 gaatcagctc gcggttctgc tgcaggttcg agtaagcctg ctggacttcc agttcaacct
     1741 ggcgcacatc gtcgtcgtag gtgatctccg cttcggaaag gagcgcccgc tgctgcttgt
     1801 agttgccgta agtcgcgccg ctatcccaga ccgcccagtt gccgaccaag ccgaataccc
     1861 agccttggcg aatctcatcc cagctcgtcg tcaaaggcga actgaatacg tcgtacccgc
     1921 cggtggtgtt gaaattcggc aaaaaaccgc cgagcgcgac gtggacttgt tcaatttggt
     1981 tcagcacggc ggcgcgttga cgcttcagga atggccggcg ctgtttgccg agctcgatcg
     2041 catccgccag cccgagcggc tgtgcgatgt attgcatctc gccttcggct tcgagcggcg
     2101 gattttctcc gcgtcgggga ataaagtcga gcccgagcgt cttagcgagc tgcaattgcg
     2161 agatgcggta attgtttcgg gctgcgatca aattcgggat ctggttcgac aaggcaacct
     2221 gcgcctgcaa gacattgaag cgtggcacgg tcccggcctc aaatcggttt tgctggtcct
     2281 tgagctgact ctcgagcagg cgcaccgatt cctcctgcac accgatgagc gccttgttca
     2341 atatgatctg gtagaactgt gtcttggtcg tggcgatgac ttgatcgacg atgtcgcgga
     2401 acccgaagta agcgttgtcg cgctggaaaa acgtgccacg aatcgagcgg aaagtgctgt
     2461 agttgaagaa cagctgagtc ccagtaatgc ggatcgcata ggaagtgtct gtcccaccgg
     2521 ggacaccgcc accaactcct ccgctagctg cgaaccctga cgagccaagc gccggatcag
     2581 tccagttgag attcgcagtc ggtgcgacgc gcggcagcgc ctccgcgcca atctgcagaa
     2641 ttacgccttt gcttcgttcg atctcctgtt tggcattgag gattgaggga ttctgcttga
     2701 gcgcggtgag aatcgcccgg tccagcgtga atgttggcgg atcagtgtgg gaaccctgcg
     2761 tcccgctgcc acgtgcgccg ccggtgctga cgccgccaag cgacaacggg ctcgctgcag
     2821 tgacgccaac gaataggctg aggacgcaaa aagtttttcg catagaatca gagtaccgag
     2881 aattggatgc gcgaaaatta gcggccggat tcaagcggca acgctttcgc tttctctgat
     2941 agcactgcag tcgatgggag aacagtcatt tcgactggca gtaatcgtaa attttactgt
     3001 agatatggag ccaactcttc tgttcgtccg gtgtgaggtg ccccatgagc ttcattaaat
     3061 tgcccaccat ctcttcgcga atgcgcctga ccaaggccga acccttcggc gtgatgcaca
     3121 ccatcacttt gcgccggtcc tcgcgtgcga ccgagcgcac cacgtagccg agattttcca
     3181 gccgcgccac caacccgctc gccgccgcag tcgtatgccc catcttttgc gcaatcgctg
     3241 acattgtgag cacttccttt tggtcgagat aagcgagcag gaaaaactgc ggaaaggaaa
     3301 cgttgcccgg agccagctcc ttgcacagat gcagcacgaa acaacgctgc atctcgagca
     3361 cgatgtgcga cagccgctca gcatccgcgc gaatctgctc attttcctca cgtgcgcgtg
     3421 ggctggcggc caaagtgctc aaagtgcgta aatggtaaga tgcggcggca cgctggtcaa
     3481 ccgaccatga ccgccgtatc cggctcaatc ccaaatctct aggtcaaccg tcgcgcccct
     3541 ggcaacggct gccgcgaccg gcagccgcag cagcgcattc gattggctca aaccaaacag
     3601 cgcgtgcgat tcctgacggc cgaccggcgc gaagttaccg tgttcgagct tgccgcgaat
     3661 gtaatgaacg cggtcgctct cattcgcaag atcgacaacc aacgtcgccc gcgtcgttgg
     3721 cagttccaga tcgcccgcgc ccattaattt caggatcgcc ggcctaacga attttaaaaa
     3781 cgtgataaac gccgataccg ggtttcccgg cagaccgaac acgagacagt tctcaagccg
     3841 gccgaacaaa aacggcttac ccggcttaat tgccacgcgc cagatgtcga tctgcgcgcc
     3901 gagatcacgc aagctttgtt gcaccagatc gtgtttaccg accgaaacgc caccgctcac
     3961 aatgaagact tggtatttgg tcgctgcgtg aaatgcctcg cgcaaggctg tggcgtcatc
     4021 ggtacagtgc tgcgtcagca tcaacttcgc gccacaacgc tgcagcaatg agcccagcag
     4081 tactgaattg ctttcgtaaa tctgaccggc ctcgagtttg tcccccgggc gcgccaattc
     4141 atcaccggtc gaaataatcg cggcctgaac ttcgccgccg acgacaatct cggccaggcc
     4201 ttgtgaggct agcaaaccaa gcgtaaccgc gcgaactcgc tcgccccctc tgatgacctt
     4261 ctgcccttca cccagatcgc agccacggcg ccggacaaat tcgccggaag taatgtggac
     4321 gttcacaaca acctcgttac cgtcagccgt gacgtcctcc tgcattacca ctgcatctgc
     4381 gccctgcggc aagggggctc cggtaaaaat gcgaacggct tcgccagccg ctacccgaag
     4441 atgtcgatca aagccggccg gctgttcggc gatgacgcgc aaccgtgacc cgggcgcgca
     4501 gtcgcttgcc atgaccgcgt aaccgtccat cgccgagtta tcgaacagtg gcagtggcac
     4561 gcgcgcgaaa taatcttgcc cggcaaaaca atccagccct cgtaagatcg acaatcggcg
     4621 cgtcgataat ggtcgaacgc tgtcgagaat cgtcgcgcgc gctttttcct cgctagtcat
     4681 gccaaactgt agcggcgatc tctgatcgtt gcaccgattc ctccgtcaga atacagccgc
     4741 gacaaagtgg gaatggacaa ttggcaatca acaattccgc ggcatattct cggctcgagc
     4801 ttttggtgag gtggctgagt ggttgaaagc agagctttgc taaagctccg tgcctcaaaa
     4861 gggcaccgag ggttcgaatc cctccctcac cgccatgtct ggtgccccct caactcccct
     4921 tcttctgcgc cacgttttga tctaagagca aggtttgtct gagattttgg caggatgctc
     4981 gtcaatcctc atcagcgtct ttcgacgccg ggctgttacc tggcagcgac gctgctgccg
     5041 acgacccgct tcatcgctgc ctcgagccgt tcgaggttca ccggtttggt caggtgttcg
     5101 gcgaagccgg cttgcaggct tctctcgata tcgccattgg tgccgaagcc gctgatcgcg
     5161 attccgggca tgccgcagat ggcgcgcacc tgcatcatca ggttcatacc ggtgccgtcc
     5221 gggagcgcga ggtcgctgat gaggagatcg aattggttgc gaaccgcggc ttcgagcgcg
     5281 gcgcgcacgt tgtgcgtggc ggcgacgagg tgaccgcgac ggacgagtaa tttctcaagt
     5341 gcggtacagg tgtcgtggtg gtcatcgacg agcaggatgc gcagagtgcg ggaaggaaca
     5401 ggcgctgcca cggacgcggt cggacgctgc tgtggtggca cagtattgag cgttagcaaa
     5461 aacgtggtgc ctttgtccct gccgggactg cgggcgacaa gtgtgccgcc atgcgccttg
     5521 gccaacgact tggaaatggc caggccgagg ccgaggccgc cgaaacggcg gcgcaaggtc
     5581 agttcgccct gctcgaaggg gtcgaaaact cggtcgatga tctctggatc gatgccgatc
     5641 ccggtgtcgc gaacgctgat cgtgaaagtt tgcggcgcgg gattcgtgga tgagatgtta
     5701 atttcgccgt tctcgggcgt gaatttgatg gcgttcttga gcagattcca gatgatctgc
     5761 tgaaatttgg ccgaatcgcc ggagatatgg tgcgcgccgg cgcggagatc ggaatagagg
     5821 tgcagttgct ttgcattcgc ttcggcgcgg cagatgtcga tcacgttcag gacggcttcg
     5881 tgggcgtcga gtgtgtcaaa tttcaactcg agtttgtctt tcgcgatgcg ggtgagatca
     5941 agaaggtcgc cgatgagctg attttcgagc tcaacgttgc ggcgaatcat cgccaacgcg
     6001 gctttggcct cttcggtttt cgccgaatcg atctccaacg agtcgagtgc ggtaatcaca
     6061 ggggtaagcg gagtgcgcag ttcgtgtgaa agcatcgcca gaaaattatc cttcgtccga
     6121 ttggcggttt cgacggcgct cttttgctgt tcgatcgccg cttcagcccg gcggcgttcg
     6181 gccatcgcgg cggagagcgc catcgcggta atggtgagca ccgctgtcca ggtttgaagt
     6241 gtcagtaacg actggttctc cgtatccatc acaaacgggc ctgcgccatg cagcgttccc
     6301 caaatcgcga tcgccgagag gatgaaaatt cccgtcgccg tttcgcgctg cgaaaatcgg
     6361 aaggccatcc agatgacgat cggtccacag atgaaggaga tcgggtaatt tctcgccgag
     6421 atcgatgacc agccgccaaa aacaatttca gccagacaga cgagtaagaa caaaagaatg
     6481 ctaacttcga tagcttctct tcgcgtccag cgccgcggag acggaatact ccagagtaag
     6541 atcaaaggcg tgatgatcag atcgccggcg gcatcgccga gccaccatgt caaccaaatg
     6601 ggccaatagc tggtccaact ggcgaagtcg ccgagcgcca gggtggtcac gccgatgctc
     6661 gggcttaaga gcgtgctgag cattgctgca atcgcaaact ggaaaacacc gcgggcgcgt
     6721 tcgaacacct tggtgccgcc ggcgaaatgg ttaaccagcc atgcgccaca aacagcttcg
     6781 agcgtattac cgctcgcgat tccaagggaa gtcaggacat tcccggccgt ggtcgcgttt
     6841 accaaaaacg cgccgatgaa aatcgccggc cacatgcgat agccgagcac cagcagcgcc
     6901 gccaacgcga tgccggctgg tggccaaacg ggcgatgcac ttcgatggag aaacgccaga
     6961 tgcagcccca gctttccggc tacgaaatac agcgcggtga gcgcagcaat cgccgacacc
     7021 gtggagaagc gtttaggagg caccatcacg cgagcgcgtt cgcgaatcgg ctacccttac
     7081 gctgctcctc gacacggtca accgatttgt gaatagatgt gaataagtct cgccgcgtcg
     7141 atctcaagat cgacaatcac tcgcggtgtc atcctgagca gagcgtagga cctcgcacaa
     7201 actcctgggt cacgcttgga aaatcgcttg acgcaagctt cctatgtgag gtccctcact
     7261 tcgttcgggg tgacgaattg agcgcgccgc gacgtcagcc gcacgcgagc gaagcaatca
     7321 gccaggctga caatcagtaa tcccgcacgt acggatattt tgtttcgtta ccgagctcgg
     7381 gcgggaactc cgaataaccc gcgtgaatcc agggaatatc gctcggcagc agcgggtaat
     7441 aggtcccgtg ctgcgtcggg aacggcgcaa gcaccgcttc aatgaagccg gccacgtggc
     7501 gcaccgccga gcgcccgaag ccgcgcacca tgccatatcc gaacgcggcg ttgttgccct
     7561 cgtccttgtt gatctggcct acggtggaaa agatttcagc cgagccgagg aagaagttgg
     7621 acaggccgcg gccgagcttt cgtgtcggcc cataatcgtt tccaggtggg tcttggatat
     7681 cggcggcagc agtgccaacg aacgccaccg cgattgcaag cgcgagtaac gttttcatag
     7741 ccgactctaa taaaccgaaa tcgtgacccg aaaagcaagc ctttatttta gccgtttagc
     7801 tgcggcacgg ttccgcgtcg cgatcattga catcatgatc gtgacagcga gaacgaggac
     7861 gatcacgaga agcgagatgc ctgtcggcac gtggacaaca tctgcgattg tcatttttac
     7921 gccgacgaag ccaagcacga tggctaggcc agttttcagg taaacgaagc gatcgatcag
     7981 acgggcgagc agaaagtaga gcgaccgcaa gcccagaatg gcacaaatat tcgaggtata
     8041 gacgatgaac gggtcctgag taatagcgat aacggcgggg atggaatcaa tcgcgaaaac
     8101 caggtccatc acatcaatca cgatcaacgc cagcgccagc ggcgtgagca tccaacgatc
     8161 tgcaatccgc accttgaaat ctgcgttgtg aaactctggc gtgatcggca accacgtctt
     8221 gcaaaatcgc atgaccaggc tttttccgaa atcctggtcg cgcgtctttc ccaggagcat
     8281 ccgaattgca gtgatgatga gaaagactcc gaagagatag acaacaaaat gaaagcgctc
     8341 caccaatgct aggccgagcc agatcatgac gccgcgcatg accagtgctc ccacgattcc
     8401 ccagaccagc acgcgatgtt gtgcgatggg cgggacgccg aaataggtaa agatcagaat
     8461 gaagacgaaa atgttgtcca cgctcagcga gtactcgatc acgtagccgg tcaagaaatc
     8521 gagcgcgtga tcggggccgc ggattttcca gacgatcaag ttgaagccta acgagaggag
     8581 aatccagatc gccgtgcgct gcgccgccgc acgcatgctc aaagggcgcc cgcgatgttt
     8641 gaactgcgca agatcgacaa tcagagcgaa gaacacgcca atgtgaaagg cgacccagaa
     8701 ccagatgctc gtcggcacgc gtgagacttt catgcgcaca accgcggagc aatcgcaaat
     8761 tgcgcgcacc gctcagacca cctataggtc acatgcgact tgcatcgcac atcccatgga
     8821 cgctgagttg gagaaactcg tcgaggccgg taaactgacc gataaggccg ccgaacggct
     8881 cgaacagctt aagcccggca ctttctgcct gcacaaaagc tggggcttcg gccgcgtcgc
     8941 cgaatggaac ctcctgctca atcaaatcgt catcgatttc gccgccaaaa aagcgcacgc
     9001 catgcaattg caatacgccg ctgactatct tacgccgatt ccgccggaac actttctcgc
     9061 ccgcaaggcc gacgacctca ccgggatcaa gcaactggcc agggatcagc cggtcgcgct
     9121 cgttcgcaac attctcgaaa gcctcggtgg caaagccacg ccgcaacaga tcggtgaatg
     9181 gatgatcggc gacatttttt ccgagccgga atggaagcgc tggtgggaat cgaccaagaa
     9241 gctcctcaga aaggacggac acttttccat ccctgcaaaa aagaacgagc caattgaatt
     9301 gcgctcggct ccggtctcgc gcgccgacga attactcgcc cggttcacgc acgcgcggca
     9361 accgcgtgag cagatcgccg ccttggacga aatcgtcaag tcgcaccagg aattcaacga
     9421 gccggagaag caattgcagc cggtgatcgc cgcgatcgag aacgcggcgg cgcgcaatca
     9481 aaaattgcat ccggagctga gcttcgagtt tgtgctcggc cgagacgatt tatcccagcg
     9541 gtttccgcag ctcaagacga cccacatcgg tctcaccgtg ggcaaactga tcgaggacga
     9601 agaacgacgc ttgatgtcga tcttgccgaa gctggcggcg gcgaaagaga aacgcatcct
     9661 gcaaacgctg ccaactgcgc tgggtgaacg ctggagcgcg cgggcattgc aattgatgca
     9721 ggcgacgcat ggtcgaatgg tcgcgcagat tccgcacgtc ttccgcgatg ccggtcagca
     9781 cctggagctg caaaagatgc tcgagcgcag catccgggag cattcggcca cgagtgaaat
     9841 gctcacctgg ctctgcgccg aacgtaacga atggcgcgaa ttaatcacgc ccgaattgct
     9901 ctccgcgatt ttatccgcgc tggaacgcga gcaacatcag gcccccgggc gcgccagtaa
     9961 actgcagcgt ttactcatcg aagatcgaca attgatcgct gacattttcg ccgacaccga
    10021 tgtggcactc gcgcgcgatg cgatgcggcg tctgcagttg actccgttgt tcgacgaact
    10081 aacgaagcgc tccttgctcg cgcggcttgt caaggtttac ccggaactcg aaagcatgat
    10141 cactggcgcg aagccggagg agaaggcggc tccgcttgtc gtttcctgga gtagtctcga
    10201 caagcgcaaa gctgaatacg aggaattggt gcagagaaag atcccggaga acacgaagga
    10261 gatttcggtc gcacgttcct acggcgacct tagcgaaaat tttgaattca aggccgccaa
    10321 gcagatgcaa gccgtactga tgcggcgcaa aaccgagctc gag
//
LOCUS       DASTDG010000217         6443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_961469, whole genome shotgun sequence.
ACCESSION   DASTDG010000217 DASTDG010000000
VERSION     DASTDG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961469"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..3670)
                     /locus_tag="VFO30_07160"
     CDS             complement(260..3670)
                     /locus_tag="VFO30_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET9419102.1"
                     /translation="MKRVIEHPPESPMGKRYWRSLGEFANTPGFREWLEREFPAGASE
                     LGDGEWSRRGFLKLMGASMALAGVGLTSCRRPEAHLVPFTKSVEWTIPGKALYYATAM
                     PRRGGAIPLIATTVDGRPIKLEGNPLHPASSGTTDLFAQASIIDLYDPTRARRFVNKG
                     KTSDRATFEKYVVDLTSKAKADGGAGLAFLVEEVQSPTRERLRGELERMFPAMRWCVY
                     DAGLTEDQPAATQAAFGPNARLIPRLDRADVILSLDSDFLDCGDGDLAAVRGFTNRRR
                     ISEAKETMNRLYVVENRFTLTGSMADHRLRSPASQIATVAYALAREIGSATGDAGLTS
                     LLGTISPTVTEGLDEEWIAEAAADLASKPGASLVLVGAHQPAAVQLLGYAMNAALKNV
                     GATLGVRDQPRNPRTLTLLQLADEIAHDRVKQLFIFGGDPVFNAFRTLVRDPATNQPL
                     DWVDLQKKVPDVVRLGYHEDATSATSTWHVPLAHYLESWGDALASDGSYLSIQPMILP
                     LFGGVSEVELLNMLLGRAKVEGPELIQETFRATSPPGDFAMAWTKFVHDGFAAHIITH
                     DRGALAIAGGAVTAAQHSWTSTSAPTPQSPEIVFVRDYSMDDGRYVNNGWLQELPDPI
                     TKLAWDNAALMSPAMARQLGVVDGDLIRITIVDVARDQNNTPLKRELEIAALVSPGHA
                     DNSITIPIGYGRRMPGFDALPYAGGAVIEHPEMEEQRGFNAYLLRTAANPHFVVADGK
                     NVESVRVEKVGRKYPLAITQEHWSIEGRGLVREATLEHYRAENDFVKKVEGEEELPSK
                     LPSLYAHPPLQSEQQWGMTVDLNVCTGCSACVIACQAENNIPVVGKLQAAHGRSMHWI
                     RIDRYYASERGFNQDNGEWPQNPEIVHEPMLCQHCENAPCETVCPVNATIHSEDGLNV
                     MAYNRCVGTRYCANNCPFKVRRFNFFDYNQRPVGKKKIAGQFATYHEYWAPFTEKGAP
                     DIVKMQKNPNVTVRMRGVMEKCTFCVQRIEEAKIAAHVRAGASKNTRIPRDSFTVACA
                     QACPNEAIVFGDIADPESKVSKMKTQDRNYRLLQYLNVNTRTSYLARIRNPNPQMPDA
                     DKIGVASPAEKPRTENVHHNYEEQHDKGKLNPNSDREPEGEETPAPHG"
     gene            complement(3816..4586)
                     /locus_tag="VFO30_07165"
     CDS             complement(3816..4586)
                     /locus_tag="VFO30_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9419103.1"
                     /translation="MAAPVVLSALPLIRMANFFPRWTNWLPLKIAICGVLIVCGLTAG
                     TWYYVTPKYTRVGYEPIQPVPFPHDIHVTQLGMDCRYCHSFVEVAAHSNLPNTQTCMN
                     CHSQVQKDNPKLEPVRVSWKTGNPIEWVQLHRTPDYVFYNHSAHVNRGISCFSCHGPV
                     NHMPVVYHAKPHSMAWCLECHRNPENFLRPEDQIFNLDWKPDEVKPAEFVAKYGAPHG
                     ADPAELAKKKKLTQQEIGLTLKEKWDIIPPTNCQGCHR"
     gene            4616..>6443
                     /locus_tag="VFO30_07170"
     CDS             4616..>6443
                     /locus_tag="VFO30_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419104.1"
                     /translation="MWGVQTMQSFRLIACPREEQKSINARRPGKRKFVAPFPVHVLPV
                     KEHVAQTKRGKWIVGRGRIEMFWKPPTFPTREELDQIALDERCLDFARHEKVATGAMS
                     CRKFPDDPVRVRSALLLCLFFAGVGDLSLPLSAQEHEHPVPEKLGVVHFPTSCSAAVQ
                     KDFERAVALLHSFAYSAAEKTFRDVANADPKCAMAHWGMAMTYFHPLWPPPLPEENVA
                     RGHEEIARARQLGGSERERGFIEALSVIYMNADSVPYFDRAKRYAEAMGKLAQRNPDD
                     AECQIFYALALLTTAPPTDSTHANQKKAAALLEPLFNKYPQHPGVPHYLIHACDNVEM
                     ATRGVDAARVYAQIAPSAPHALHMPSHIYTRLGMWRESIASNAAARNAARAQDDIGEE
                     LHAMDYLTYANLQLGRDQDAAQVLDDLRKMTNLSPKYFKVAYAASAMPARYAIERRKW
                     NEATQLVAIPDALPQAGAITAWSRAVGFARGKQTVPARKEAEKLMGAYEQMRAAGDEY
                     WARQIHVQLNSALAWINYAEGKSDDAVKLLRTAADEEDGVEKLPVTPGAIVPTREQLG
                     DLLLELNQPADALKEFERVLTMTPQRRGAITGASRAREMLTKR"
BASE COUNT         1291 a         1915 c         1887 g         1350 t
ORIGIN      
        1 gtcacccacc accagcgcgg cgcccgttgc tcgaccaccc cggaaatgcg ctccgtgatc
       61 cagctgaggt tgcgattatg caaaaccagc ggcggacgtt tgacctcctc ctcgaccgtc
      121 gtcgacgctt ccgcccgaat tgtctctgga gctgtagggg cgctatccat gttcgtacag
      181 tggcatcggc atcttgccga tgggacatgc gctggaagcg catgccactt tgcaagatcg
      241 aaatctcata acatcgatgt cacccatgcg gcgcgggcgt ttcttctcct tctggttccc
      301 ggtccgagtt cggattcaat ttccccttgt cgtgctgctc ttcgtaattg tgatgcacgt
      361 tttctgtgcg tggcttttcc gccgggctcg ccacgccgat cttgtcggcg tcgggcattt
      421 ggggattggg atttcgaatc cgcgcgagat aactcgtccg cgtgttcacg ttcagatact
      481 gcagcagccg atagttgcgg tcctgcgtct tcatcttcga gactttgctt tccggatcgg
      541 ctatgtcgcc gaagacgatc gcttcgttcg ggcaggcctg tgcacaggcg accgtgaatg
      601 agtcgcgcgg aatgcgcgtg ttcttcgacg cgccggcgcg gacgtgcgcc gcgatcttcg
      661 cctcctcgat gcgttgcacg cagaaggtac acttctccat caccccgcgc atgcgcaccg
      721 taacgttggg gttcttttgc atcttgacga tgtcaggcgc gcctttttcc gtaaacggcg
      781 cccagtattc gtgataggtg gcaaattgcc cggcgatttt ctttttcccg accggccgct
      841 ggttgtaatc gaagaaattg aagcgccgga ccttgaacgg gcagttgttc gcgcagtaac
      901 gcgtgccgac gcagcggttg taagccatga cgttgagacc atcctcgctg tgaatggtcg
      961 cgttcacagg acaaacggtt tcgcacggcg cgttctcgca atgctggcaa agcatcggct
     1021 catggacgat ctccgggttc tgcggccact cgccgttatc ctgattaaat ccgcgttcgc
     1081 tcgcgtagta ccggtcgatc cgaatccaat gcatggaacg gccgtgcgca gcctgcaatt
     1141 tccctacgac cgggatattg ttctcggcct ggcacgcgat cacgcacgca ctgcaaccgg
     1201 tgcagacgtt taggtcgacc gtcatccccc actgctgctc cgattgcagc ggaggatgcg
     1261 cgtaaagcga cggcagtttc gaaggcaact cttcttcgcc ttcgaccttt ttcacgaaat
     1321 cgttttccgc gcggtaatgt tcgagcgtcg cttcacgcac gagtccccgg ccttcgatgc
     1381 tccaatgctc ctgcgtgatc gcgagcggat atttgcggcc gactttttcc acccggacgc
     1441 tctcgacgtt cttgccgtcg gcgaccacaa aatgcgggtt cgccgcggtg cgcagaaggt
     1501 aagcattgaa ccctcgttgc tcttccatct cggggtgttc gattaccgcg ccccccgcgt
     1561 acggcaaagc gtcgaatccc ggcatgcgcc ggccgtagcc gatcgggatg gtgatcgaat
     1621 tgtcggcatg accgggcgag acgagcgctg cgatttccag ctcgcgtttg agcggcgtgt
     1681 tgttttggtc acgcgcaaca tcgacaatcg ttatacggat caaatcgcca tcgacgactc
     1741 ccaactgccg cgccatcgcg ggactcatta gcgcggcgtt atcccaagct agtttcgtaa
     1801 ttgggtcagg cagctcctga agccagccat tgttaacgta gcgcccgtca tccatcgagt
     1861 aatcgcgtac gaacacgatc tccggcgatt ggggagttgg cgcactggtt gaagtccacg
     1921 aatgttgcgc cgctgtgacg gcgccgccgg caatcgcaag tgcgccgcga tcatgcgtga
     1981 taatgtgagc cgcaaaaccg tcgtgaacga atttcgtcca cgccatggca aaatcgcccg
     2041 gcggacttgt cgcgcgaaat gtctcctgaa tcagctccgg tccttctact ttcgcgcggc
     2101 caagcagcat gttgagcaat tcgacttcgg agacgccgcc aaagagcggc aggatcatcg
     2161 gttgaatcga caagtaggag ccgtcgctcg cgagcgcgtc accccacgac tccaaataat
     2221 gcgcgagcgg cacatgccag gtgcttgtgg cggatgtcgc gtcctcgtga taaccgagcc
     2281 gaacgacatc cggcactttc ttctgcagat ccacccaatc gagcggctga ttcgtcgccg
     2341 gatcgcgcac gagagtacgg aatgcattaa aaaccgggtc gccgccgaag ataaaaagtt
     2401 gtttcacccg atcgtgcgcg atttcgtcgg cgagttggag caaggtcagc gtgcggggat
     2461 ttcgcggctg gtcgcgcacg cccaaggttg cgccgacatt cttcagcgca gcgttcatcg
     2521 cgtagccgag caactgcacc gcggccggtt gatgcgcgcc gacgagcaca agacttgcgc
     2581 ccggttttga ggcgagatcg gccgccgcct cggcgatcca ttcttcatcc aaaccttccg
     2641 ttaccgttgg cgagatcgtg cccagcaatg aagtcaatcc tgcatcgccg gttgcgctgc
     2701 caatttcgcg ggcgagtgca tacgccaccg tcgcgatttg gctggccgga gaccgcagcc
     2761 ggtggtcggc catggaaccg gtgagtgtga accggttttc caccacatag aggcggttca
     2821 tcgtctcctt tgcttcactg atgcgccgcc gattcgtaaa gccacgaacc gcagccaaat
     2881 cgccgtcacc gcaatcgaga aaatcactat ccagcgagag gatgacgtcg gcgcgatcga
     2941 gccgcggaat caagcgcgcg ttcggtccga acgcggcttg ggtcgcggcc ggctgatctt
     3001 ctgtgaggcc ggcgtcataa acgcaccagc gcatcgccgg gaacatgcgt tcgagctcac
     3061 cgcgcaagcg ctcgcgcgtg ggcgattgca cttcttcgac aaggaacgcg agaccggcac
     3121 cgccatcggc cttcgctttc gacgtaagat cgacaacgta cttttcaaac gtcgcgcgat
     3181 ctgacgtttt gcctttgttg acgaaccggc gcgctcgggt cgggtcgtaa agatcaatga
     3241 ttgaggcttg cgcaaaaaga tcagtcgtgc cgctgctggc cgggtgcagc gggttgccct
     3301 cgagcttgat cggacggccg tccacagtgg tggcaatcag tggaattgca ccgccgcgtc
     3361 gcggcatcgc cgtggcgtaa tacaaggctt tgccgggaat cgtccattcg acgcttttcg
     3421 tgaatggaac aaggtgcgct tccggccggc gacaacttgt taggccaaca ccagccagcg
     3481 ccatcgacgc gcccatcagt ttcaggaaac cgcgccgcga ccattcgccg tctccgagct
     3541 ccgaggcgcc cgccggaaac tcgcgctcca accattcgcg aaatcccggc gtgttcgcaa
     3601 attcacccag gctgcgccag tagcgtttcc ccattggcga ttccggcgga tgttcgatca
     3661 cgcgcttcat cggagcatag acctccggtc tgtgcggccg acaggcatct cgcctgtcga
     3721 acctgaaaca aaaaagtcag gctgaaagcc cgatggccac acaggcgcaa cgcctgtgtt
     3781 ccgacgcggc tccggcggat gttcgatcgc gcgcatcatc gatgacatcc ctggcaattg
     3841 gtcggcggga tgatatccca cttttctttc aaggtgaggc cgatctcctg ttgcgtgagt
     3901 tttttcttct tcgccagctc cgccgggtcg gcgccatgcg gcgcgccgta cttcgccaca
     3961 aactccgccg gcttcacctc atccggtttc caatcgagat taaaaatctg atcctcaggc
     4021 cggagaaaat tctcgggatt ccggtggcac tcgaggcacc aggccatgct gtgcggcttc
     4081 gcgtggtaaa ccaccggcat gtgattgacc gggccgtggc aactgaaaca actgatcccg
     4141 cggttaacgt gggcggagtg attgtaaaac acgtagtccg gcgtgcgatg cagttgcacc
     4201 cattcgatcg gattaccggt cttccagctc acccgcaccg gctcgagctt tgggttgtcc
     4261 ttttgcacct gggaatggca gttcatgcag gtctgggtgt tcggcaggtt tgagtgcgcg
     4321 gcgacttcga cgaagctgtg acagtaacgg caatccatcc cgagctgcgt cacatggatg
     4381 tcgtgcggaa acggcaccgg ctggatcggt tcatagccaa cgcgggtgta ttttggcgtg
     4441 acgtaatacc aggtgccggc cgtcagccca cagacgatca gcacgccgca gattgcgatt
     4501 ttgagcggca gccaattggt ccagcgcggg aaaaaattcg ccatacgaat cagcggcagc
     4561 gctgaaagca cgaccggagc cgccaacctg aaaacgaatc ggacgcgaga ttaggatgtg
     4621 gggcgttcag acgatgcaaa gtttccggtt gattgcctgt ccgagggaag agcaaaagtc
     4681 tataaatgcg cgtcgccctg gcaaaagaaa attcgtcgcg ccttttcccg tgcatgtttt
     4741 gccggtgaaa gagcacgtcg cacaaacgaa gcgcgggaag tggatcgtcg gccgcggccg
     4801 gatcgagatg ttctggaaac caccgacatt tccgacgcgc gaagagctcg accaaatcgc
     4861 cctggatgag agatgtctcg acttcgctcg acatgagaag gttgctacag gggcgatgtc
     4921 gtgcagaaag tttcctgatg atcccgtgcg agtgagaagc gcgcttttat tgtgcctgtt
     4981 ttttgccgga gtcggcgact tgtctttgcc tctgtccgct caagaacatg agcatccagt
     5041 tccggagaag ctcggcgtcg tgcatttccc gacttcgtgc tcggcggccg tgcaaaaaga
     5101 tttcgagcgt gccgtggcgc tcctgcattc ctttgcttat tcggctgcgg agaaaacttt
     5161 ccgcgatgtc gcgaacgcgg atccgaagtg cgcgatggca cactggggaa tggcgatgac
     5221 ttattttcat ccgctttggc cgccaccgct tcctgaagag aacgttgccc gcggtcacga
     5281 ggaaattgca cgcgcccgac aactcggcgg ctcggagcgc gaacgcggat tcattgaagc
     5341 attgagtgtc atttacatga atgcggattc ggtgccgtac tttgaccgcg ccaaacgtta
     5401 cgcggaagcg atgggcaaac ttgcccaacg caatccggac gacgctgagt gccagatctt
     5461 ctacgcgttg gcgcttctga ccaccgctcc gccaacggat tcgacgcacg caaatcagaa
     5521 aaaagccgcc gctctacttg aaccgctttt taacaagtat ccgcagcatc caggagttcc
     5581 gcactatctc atccacgcct gcgacaacgt cgagatggcg acgcgcggcg tggacgcggc
     5641 gcgagtgtat gcgcagatcg caccgtccgc tccgcacgcc ttgcacatgc catcgcacat
     5701 ctacacccgt ctgggaatgt ggcgggaatc gatcgcttca aacgcagctg cgagaaatgc
     5761 cgcgcgcgcg caagacgaca taggcgagga attgcacgcc atggattatc tgacctacgc
     5821 caatcttcag ctcggtcgcg atcaggacgc ggcgcaggtc ctcgatgact tacgaaaaat
     5881 gacgaatctg tcgccgaaat atttcaaagt ggcatacgca gcgagcgcga tgccggcgcg
     5941 ctatgcgatt gaacgtcgaa agtggaatga agcgacgcag cttgtcgcga tacccgacgc
     6001 gttgccgcag gcgggcgcga ttacggcctg gtcgcgtgca gttggatttg cgcgtggcaa
     6061 acaaaccgtg cccgcgcgca aagaagcgga gaaattaatg ggcgcctacg agcaaatgcg
     6121 cgctgcgggc gatgaatatt gggcgaggca aattcatgtg cagctcaatt cggcgttggc
     6181 ctggataaac tacgccgaag gcaaaagcga cgacgcggta aagctcctgc gcaccgcggc
     6241 cgacgaagaa gacggcgtgg aaaaactacc cgtgactccg ggtgcgatcg tgccaacgcg
     6301 cgaacaactt ggtgatctgc ttctggaact aaatcagccc gctgatgctc tcaaagaatt
     6361 tgagcgtgtg ctgaccatga ctccgcaacg ccggggcgcg atcacgggag cgtcgcgtgc
     6421 ccgtgaaatg ctgaccaaac gca
//
LOCUS       DASTDG010000218         6816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1042730, whole genome shotgun sequence.
ACCESSION   DASTDG010000218 DASTDG010000000
VERSION     DASTDG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042730"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..168
                     /locus_tag="VFO30_07175"
     CDS             13..168
                     /locus_tag="VFO30_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HET9419105.1"
                     /translation="MEQRKDKKVIAFKYKRRKGYHRTVGHRRKLTRVKIKSIGGGKKT
                     ASKKSEE"
     gene            201..458
                     /gene="rpmA"
                     /locus_tag="VFO30_07180"
     CDS             201..458
                     /gene="rpmA"
                     /locus_tag="VFO30_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9419106.1"
                     /translation="MAHKKGQGSVKNGRDSVSKRLGVKKFGSEMVVAGNIIVRQRGTK
                     FLPGKNVGLGRDYTIFALIDGSVRFDRAGRRVNVDPLLATK"
     gene            545..1174
                     /locus_tag="VFO30_07185"
     CDS             545..1174
                     /locus_tag="VFO30_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET9419107.1"
                     /translation="MKYKTYILFGAPGSGKGTQGKTLGTIPRFYHCPCGDVFRSIDTR
                     TKVGRAFLEYSSKGQLVPDEITVELWQEAIDAAVDAHKFKPDIDTLVLDGIPRNVNQA
                     MLMQEMIDVQKVFHLSCPDRAALFTRLKKRALKDNRLDDANEQVIQKRLEIYERESKP
                     VLGFYPRKCITVVDATQPPAKVLFDILASLNGVNGAVEHHESAAAAAAV"
     gene            1143..1400
                     /locus_tag="VFO30_07190"
     CDS             1143..1400
                     /locus_tag="VFO30_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419108.1"
                     /translation="MSQQRRQQQFENFTASSLYCEKCKAAMPVRERLLLVLPDRDIFD
                     YLCTGCASSVGQREVTAGEKIMMQATQPRTRRAIPLKPAIR"
     gene            1410..2339
                     /gene="fmt"
                     /locus_tag="VFO30_07195"
     CDS             1410..2339
                     /gene="fmt"
                     /locus_tag="VFO30_07195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET9419109.1"
                     /translation="MRVVFIGTGEIGVPTLESLLNANEHELVGIVTGPDKPVGREQRI
                     EPSPIKAAVAGKNIGTVQPARIKDRQAIDRIRALNPDLIAVMAYGQILPRDVLEIPKI
                     ACVNLHASLLPRWRGASPIQAAIATGDRETGITVMYMNEGLDTGDVLLQRKIDISPNE
                     TGRSLHDRLAQIAPDALLEALRLLASGSAPRIPQNDAIATYAPKLTREGGRIDWSEPA
                     EVIERKIRAYNPWPGASMKLDNRSLKIFSAAIVDFAGKPGEVLGADKEFVVAAGEGAL
                     SLLELQLEGGKRMRAAEFLRGQRWIAISGRDAQ"
     gene            2432..3172
                     /gene="pyrH"
                     /locus_tag="VFO30_07200"
     CDS             2432..3172
                     /gene="pyrH"
                     /locus_tag="VFO30_07200"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9419110.1"
                     /translation="MEKETPAYKRVVLKLSGEALQERGARDNISPAVVREIAERIKEV
                     NQLGVQIAVVIGGGNIWRGLSASHRGMDRTTADYMGMLATVINALALRSALAQLGVET
                     RVQTAIEMKNVAEPFILGRAIRHLELGRVVIFAAGTGNPYFSTDTTAALRASEIRADV
                     ILKATKVDGVYDRDPTVDANAKRYDQITFTEALAKRLQIMDSTAFSLCMDNKIPIVVF
                     DMYKPGILREAVLGRPVGTLVCGDPEPK"
     gene            3183..3743
                     /gene="frr"
                     /locus_tag="VFO30_07205"
     CDS             3183..3743
                     /gene="frr"
                     /locus_tag="VFO30_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9419111.1"
                     /translation="MTRDDVLLEAEMSMEKSVDYMVHEFAGVRTGKASPALVENVDVQ
                     AYGASMKLKQLALITTPEARLLVVQPFDAGTVQDIERALKESKIGITPAVDGKIIRLP
                     IPELSEERRKELVRSLGKMAEEARVRVRSNRRTAMDEAKKLKAAGQLAEDALRDLEEE
                     VQELTDRFVKSIDDHLKHKEAEVMKV"
     gene            complement(3820..4635)
                     /locus_tag="VFO30_07210"
     CDS             complement(3820..4635)
                     /locus_tag="VFO30_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419112.1"
                     /translation="MLFGLFLTLGVAVLSVALRTFQSSFSQKTGALGILISSYLAVYF
                     ITSSWILGVVAAVSWLFLPWLEILTRIRTLRLPREKQLRPKSPPSSEIFPNLNEITRE
                     IENEGFVHVNDAGWDWEDYRQFFRLFYKREDRAQATICLNEQHDLSFYYLRISSRGKS
                     GTIWTTWNYPLSYGLKLTPQFRINRQRPDQSFWQLYQSHREFLRRNSVDVGAIDNLDE
                     ERIQAEIEKDLRDQIAHNINKGLLKQTASGDVKYSWRGMIYLWCQFLIDLVRL"
     gene            complement(4635..4880)
                     /locus_tag="VFO30_07215"
     CDS             complement(4635..4880)
                     /locus_tag="VFO30_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET9419113.1"
                     /translation="MELKLYSREWCSWCMDAKEYLSERGYKFTEIDVGQDRQAYEEMK
                     QLSEQTYVPTFVAGDKVLANFDTDQLEGFLRKHEIRP"
     gene            complement(4925..>6816)
                     /locus_tag="VFO30_07220"
     CDS             complement(4925..>6816)
                     /locus_tag="VFO30_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9419114.1"
                     /translation="LFRQAKTGELHGFIADGQRVFSRIAALKIPTVAAIHGACAGGGY
                     EITLACDYRLASDDPATRIGLPETTLGLVPAWGGCTRLPRLIGLDAACEVILNGKLYA
                     ALEALKLGLVDEVVSPEHLVDAARNKIATGKRSMLAPPHIEKQPKPPPANGNAAPRRA
                     FEIIRQAVGSSVEESLRRELEAIVDLGKTEGTQNLIRNFFLAEKYKKGDGKFSQPKVV
                     HAAVIGAGVMGSGIAQWLSARGVTVILRDVSRELLDRGLANIEKIYSDAVKRGLMTED
                     KAKGGRARIVASTAPMELRDVQFVIEAASEKIDVKKHIFRELSMQVGPTTIVATNTSA
                     LPVSELAACTVTPERVLGLHFFNPVSRMKLVEVVIAAETSAGTQERALAFVRQIGKLP
                     VVVRDTPGFLVNRVLFPYLLDAAELFEAGVQANKIDNALVNWGMPMGPLRLIDEIGVD
                     VTVDIATTLEKAHGRRDHAATVLHWLRDGGMLGRKTGSGFYKYEGKTQTANALLTEWR
                     RGLHGDPEGAAGPVIPPNRHRDPRLQLTEEELSLRLVFLMVNEAARCVEENVVNSPED
                     ADYGMILGTGFAPFRGGPLRFAEQFGLAKIVDAMDQLARTDDKFTPCEILKRCAREGG
                     RFYAE"
BASE COUNT         1493 a         2012 c         1956 g         1355 t
ORIGIN      
        1 accgcggaag ttatcgagca gcgcaaagac aaaaaggtca tcgcgtttaa atacaagagg
       61 cgcaaaggtt atcaccgcac cgtcggccac cgccgcaagc tcacgcgggt gaagatcaaa
      121 tcgatcggcg gcgggaagaa aacggcgtca aagaaatcgg aagaatagtt tcatccttca
      181 tccttcttaa tttttgttat atggctcaca aaaaaggaca gggcagcgtt aagaacggcc
      241 gtgacagtgt cagcaaacgg ctcggcgtga aaaaattcgg cagcgaaatg gtcgtggctg
      301 gcaacatcat cgtgcgtcag cgcgggacga agtttttacc tggcaaaaac gtgggtctcg
      361 gccgcgatta cacgatcttt gcattgattg acgggagtgt gcgttttgat cgcgccggac
      421 gacgggtcaa cgtggatccg ttactcgcga caaagtaaga ggcgagttca gccaagacat
      481 tctcttcccg gtgattccgc ttcccgcccg cgggcaattt gcggtataaa cgaaacccgc
      541 tctgatgaaa tacaagacgt acatcctttt cggcgcgccc ggcagcggta agggcacaca
      601 gggcaaaacc cttggcacga taccgcgctt ttatcattgc ccgtgcggcg acgtgtttcg
      661 ctcgattgat acgcgcacga aagtcggccg cgcgttcctg gaatactcga gcaagggtca
      721 gctggtgccg gacgagatca cggtcgagct gtggcaggaa gcgatcgatg cggcggtgga
      781 cgcacacaaa ttcaagcccg acattgacac gctcgtgctc gacggcatcc cgcgaaatgt
      841 caatcaggcg atgctgatgc aggaaatgat cgacgtgcag aaagttttcc atctctcctg
      901 tccggatcgt gcggcattgt tcacgcgtct gaaaaagcgc gccctcaagg acaatcgctt
      961 ggacgacgcg aacgagcagg tcatccaaaa gcggctcgaa atttacgagc gcgaatcaaa
     1021 accggtcctc ggcttttacc caaggaaatg catcacggtg gttgacgcga ctcagccgcc
     1081 cgccaaagtt ttgttcgaca tcctcgccag cctgaatggt gttaatggcg cggtcgaaca
     1141 tcatgagtca gcagcggcgg cagcagcagt ttgagaattt cactgcctcg agtctttact
     1201 gcgagaagtg caaggctgcg atgccggtgc gcgagcggct acttttagtc ctgccggacc
     1261 gcgacatctt cgattatctc tgcaccggct gcgcttcgtc cgtcggtcag cgtgaagtca
     1321 ccgccggcga aaagataatg atgcaagcga cgcagccccg gacgcgccgc gccatcccgc
     1381 tcaaacccgc gattcgttag gcgagaaaag tgcgcgtggt cttcatcggc acgggcgaaa
     1441 tcggcgtgcc aacgctcgaa tcgctgctca atgcaaacga gcacgagctc gtcggcattg
     1501 tcaccggccc ggataaaccg gtgggacgcg aacaacgtat cgaaccgtca ccgatcaaag
     1561 ccgccgtggc gggaaagaac atcgggacgg tgcagccggc gcgaatcaaa gatcgacaag
     1621 cgatcgacag gattcgtgcg ctgaacccgg atctgattgc cgtgatggcg tatgggcaga
     1681 ttcttccgcg cgacgtgctc gagattccaa agatcgcctg tgtgaacttg cacgcgtcgc
     1741 ttcttcctcg ctggcgcggt gcatcaccga ttcaagcggc catcgccaca ggcgatcgcg
     1801 aaaccggcat caccgtgatg tacatgaatg aaggcctcga caccggagac gtcttgctgc
     1861 aacgcaagat cgacatctcg ccgaacgaga ccggccgctc gttgcacgac cgcctcgcgc
     1921 aaatcgcacc cgacgcgctc ctcgaagcgt tgcgcttgct cgccagcggg agcgcgccgc
     1981 gcatcccaca gaacgacgcg atcgcgacgt acgcgccaaa actcacgcgc gagggcggcc
     2041 gaatcgattg gagtgaaccc gcagaagtga ttgagagaaa gatccgcgct tacaatccct
     2101 ggccaggcgc atccatgaaa ctcgacaacc ggagtctgaa aatcttttct gcggccattg
     2161 tcgactttgc cggcaaaccc ggagaggtgt tgggcgcgga caaagaattc gtcgtcgctg
     2221 caggagaagg tgcgctgtcc ctgctcgaac tgcagttgga aggtggcaaa cgcatgaggg
     2281 ctgcggaatt tttgcgcggg cagaggtgga tcgcgatctc cgggcgcgat gctcaatagt
     2341 ctcggctgcg ctgcctcttg ctaccatcgc gctcggagat cgcgatccac ctttacaaca
     2401 tcgacatctt ctcgtttgat cttggccggc catggaaaag gaaacgccgg cctacaaacg
     2461 cgttgtgctc aagttgagcg gcgaagcgct tcaggaacgc ggggcgcggg acaacatttc
     2521 accggcggtg gtgcgcgaga ttgccgagcg aatcaaggaa gtgaatcaac tcggggtgca
     2581 aattgccgtg gtcatcggcg gtggcaacat ctggcgcggg ctctcggcgt cgcatcgggg
     2641 catggatcgc accaccgccg attacatggg catgctcgcg acggtgatca acgcgctcgc
     2701 cttgcgcagc gcgctcgcac agctcggtgt cgagacgcgc gtccaaaccg caatcgaaat
     2761 gaaaaacgtg gccgagccgt tcattctcgg tcgcgccatt cggcatctcg agcttggccg
     2821 cgtagtgatt ttcgcagccg ggacgggtaa tccttatttc tcgaccgaca cgaccgcggc
     2881 tttgcgcgcg agcgagatcc gcgcggatgt cattctgaag gctaccaaag tcgacggcgt
     2941 ttacgaccgc gacccgactg tcgacgccaa tgcgaaacgt tacgaccaaa tcaccttcac
     3001 ggaagctctc gcaaaacggc tccagatcat ggattcgacg gccttcagcc tttgtatgga
     3061 caacaaaatt ccaatcgtcg tgttcgacat gtacaagccc ggtattctgc gcgaagcggt
     3121 gctcgggcgg ccggtgggca cgctagtttg cggtgacccg gagccgaagt gaaaaggagc
     3181 gcatgacacg cgacgacgtt ctcctcgaag ccgagatgtc gatggagaaa agcgtcgact
     3241 acatggtgca cgagttcgct ggcgtgcgta ccggaaaggc gtcgccagcg ctcgtcgaga
     3301 atgttgatgt gcaggcttac ggcgcgagca tgaaattgaa acagctcgcg ctcatcacca
     3361 cgcccgaggc ccgcctgctc gtcgtccagc cgttcgacgc cggcacggtt caggacatcg
     3421 agcgggcgct gaaggaatcg aagatcggca tcacgccggc ggtcgacggc aaaatcattc
     3481 gcctgccgat cccggaactt tccgaggaac gccgcaagga acttgtccgt tcgttaggca
     3541 aaatggccga agaagcgcgt gtccgcgtcc gaagtaatcg ccgtaccgcg atggacgagg
     3601 caaaaaagct caaagccgcg gggcaactcg ccgaagacgc gttgcgcgac ttggaagagg
     3661 aagtgcaaga gttaacagat cgattcgtca aatcaatcga cgaccacctt aagcataaag
     3721 aagccgaggt gatgaaagta tgatctcgtc acatcgacat cttcggtacc ggccgctgcc
     3781 cgcagcggca ttacaaaccg gcgcaaacct catcggccgt tacaatcgca ccaggtcgat
     3841 caagaactgg caccagagat aaatcatccc gcgccaggaa tatttcacgt cgccggacgc
     3901 cgtctgcttg agcagtcctt tgttgatatt atgcgcgatt tggtcgcgca gatctttttc
     3961 tatctctgcc tggatgcgct cttcgtcgag attatcaatc gcgccgacgt caacactgtt
     4021 gcgccgaaga aattcgcgat ggctctgata gagttgccaa aatgattggt ccggtcgctg
     4081 gcggttgatc cgaaattgtg gcgtcagctt gagtccgtag gagagcggat agttccatgt
     4141 cgtccagatc gtgccggatt tgccgcggga ggagatgcgc aggtaataaa aggagagatc
     4201 gtgctgctca ttcaggcaga tggtcgcctg cgcgcgatcc tcccgtttat agaacagccg
     4261 gaaaaattgc cgataatcct cccaatccca gccggcatcg ttcacgtgga cgaacccttc
     4321 gttctcgatc tcgcgcgtga tttcgttcag gttcgggaaa atttcggagg acggcgggct
     4381 ctttggccgg agctgtttct cgcgtggcag gcgcagcgtt cgaatacggg tgagaatttc
     4441 cagccagggc aaaaacaacc agctcacggc tgcgaccacc ccgagaatcc aactgctggt
     4501 gatgaaataa acggcgaggt aggaggagat gagaatgccg agggcaccgg ttttttgcga
     4561 gaaggagctt tggaaagtgc ggagcgccac actcagcacc gcgaccccga gcgtcaggaa
     4621 gagcccgaaa agcatcaggg ccgaatctcg tgtttccgca aaaatccctc gagctggtca
     4681 gtgtcgaaat tggccagcac tttatccccc gcgacgaacg tcggcacata agtttgctcg
     4741 gagagctgct tcatttcctc gtatgcttgt cgatcctgcc cgacgtcaat ttcggtgaac
     4801 ttgtagccgc gttcgctcag atattctttc gcgtccatgc accaggaaca ccattcgcgg
     4861 ctgtagagct ttaattccat gcgcggcgaa aataaacgag cgcaagcctc cgccgtcaat
     4921 ccgctcactc tgcgtaaaat ctgccgccct cgcgcgcgca gcgcttcagg atttcgcaag
     4981 gtgtgaattt atcgtcggta cgcgcgagtt gatccatcgc atcgacaatt ttcgccagtc
     5041 caaattgctc cgcgaagcgc aacggcccgc cgcgaaaggg cgcgaaaccg gtaccgagga
     5101 tcattccgta atccgcgtcc tccggcgagt tgaccacgtt ctcttccaca caacgagccg
     5161 cttcgttcac catcaaaaaa acgagccgga gcgacagttc ctcttccgtt agctggagcc
     5221 gcgggtcgcg gtgcctgttc ggcggaatga ctggacctgc tgcgccctcg ggatcgccgt
     5281 gcaatccgcg ccgccactcg gttagtaatg cgttagccgt ctgcgttttt ccctcgtact
     5341 tgtaaaagcc gctgcccgtc ttacgaccga gcatgccgcc gtcgcggagc caatgcagca
     5401 ctgtcgcggc atgatcgcga cgcccgtgtg ctttctcgag cgttgttgcg atatcaaccg
     5461 tcacgtcgac gccgatttcg tcgatcagcc gcaacgggcc cattggcatg ccccaattca
     5521 ccagcgcatt gtcgatcttg ttagcttgca caccggcctc gaaaagttcc gccgcatcga
     5581 gcaggtacgg aaacagaaca cgattcacca gaaaacccgg agtgtcacgc acgaccaccg
     5641 gtaacttgcc tatctgccta acgaatgcga gcgcgcgctc ttgcgtgccg gcagacgttt
     5701 ccgctgcgat gacgacttcc acgagcttca tgcggctcac cggattgaag aagtgcagcc
     5761 cgagcacacg ctccggtgtg acggtgcacg ccgcgagttc gctcaccggc aatgctgagg
     5821 tgttagtcgc aacgattgtc gtcggaccga cctgcatcga gagctcgcga aagatatgtt
     5881 ttttgacgtc gatcttttcc gacgcggcct cgattacgaa ctgcacatcg cgcaattcca
     5941 tcggcgcggt tgaagcgaca atccgcgcgc ggcccccctt cgctttgtct tccgtcatca
     6001 agccacgctt taccgcgtcg ctgtaaatct tctcgatgtt tgccaaaccg cgatcgagta
     6061 actcgcggct gacgtcgcgt aaaatcacag ttacgccgcg cgcgctcagc cattgcgcga
     6121 tgccagaccc catcacaccc gcgccgatca cggccgcgtg cacgaccttg ggttgcgaga
     6181 atttcccgtc gcccttcttg tatttctccg ccagaaaaaa attgcggatc agattctgcg
     6241 ttccttccgt cttgccaagg tcgacaattg cctcgagttc gcgccgcagt gactcctcga
     6301 ctgagctgcc gaccgcctgg cgaatgatct cgaacgcgcg ccttggcgcg gcattaccat
     6361 tggccggcgg cggtttcggt tgtttttcaa tgtgtggcgg cgcaagcatc gatcttttgc
     6421 ccgtcgcaat tttattgcgg gctgcatcga ccaagtgctc aggagaaacc acctcgtcca
     6481 ccaggccgag cttcagcgcc tcaagcgcgg catagagttt gccgttgaga atcacttcgc
     6541 acgcggcgtc gagcccaatc aatcgcggca gacgcgtgca cccgccccag gctgggacca
     6601 ggccgagcgt tgtctcgggt aaaccgatcc gcgtcgccgg atcgtccgac gcgagccggt
     6661 aatcgcatgc gagcgtaatc tcgtatccgc cgcccgcgca cgcgccgtga atcgcggcga
     6721 cagtcggaat tttcagcgcc gcgatccgac tgaaaacgcg ttgcccgtcg gcaataaacc
     6781 cgtgcagctc gccagttttc gcctgtctga aaagtg
//
LOCUS       DASTDG010000219         6911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1011776, whole genome shotgun sequence.
ACCESSION   DASTDG010000219 DASTDG010000000
VERSION     DASTDG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011776"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..736
                     /locus_tag="VFO30_07225"
     CDS             395..736
                     /locus_tag="VFO30_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419115.1"
                     /translation="MSMETNEPPTMDNLIFGEIQVLLAEKRTSLAALRTGIAVFALPL
                     SVLSVLIATSRYYSMEKVMPLLLPLLLLNLGLIVLGSWLIYHSIHRIHRYDHRIRELT
                     QKYRSIAEFID"
     gene            948..1097
                     /locus_tag="VFO30_07230"
     CDS             948..1097
                     /locus_tag="VFO30_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419116.1"
                     /translation="MHHVARHAAICSEELASFMASSSPSARNIFPASVVYLIADTFSP
                     EQQPS"
     gene            1108..2184
                     /locus_tag="VFO30_07235"
     CDS             1108..2184
                     /locus_tag="VFO30_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HET9419117.1"
                     /translation="MKTNYRAAGVPMLAALISLAVTSYAAAQSGGFVQTNLVSDIPGV
                     AERVDSNLVNPWGIVASPSNTIWIADNGTGVSTLYSPTGAKQPSGNPLVVTIPASKTS
                     TEGGNPTGIVFNGAGGFVVTEDTRSGSSFFIFASEDGVISGWNPQVALDHAVVAVDHG
                     KREAIYKGAALGTTAGGLRLYVTNFHAGTVEIYDQNFSRIHGPTKFVDPTLPSGFAPF
                     GIANINGSIYVTYAKQDADAEDDVAGPGLGFVNVFDTQGNFIKRLISRGKLNAPWGLA
                     LAPSGFGSFGSALLVGNFGNGHINAYDPNSGAFLGQLTKAGDGRSLVIDGLWGLFFID
                     KRLFFTAGIADEAHGLFGVIKASQ"
     gene            complement(2485..3204)
                     /locus_tag="VFO30_07240"
     CDS             complement(2485..3204)
                     /locus_tag="VFO30_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9419118.1"
                     /translation="MTKEQRIALVTGANKGIGFEVVRLLARKGFRVFLGARGEDAGEA
                     AVAKLHREGEKQSYGEVTFIKLDISKPESVKRAAEEFSRHSDRLDALVNNAGILLDDD
                     KDVLTITPEIFEGTLRTNALGALLVTQAFVPFLKKSDAARIVNVSSSGGQLADGADGW
                     APAYCISKTALNGVTSQLAAALPKFAVNSVCPGWVRTDMGGANATRSVAEGAAGMVWL
                     VSEAPHSMTGKFFRDRKIIPW"
     gene            complement(3305..3679)
                     /locus_tag="VFO30_07245"
     CDS             complement(3305..3679)
                     /locus_tag="VFO30_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419119.1"
                     /translation="MKEKMIALACITIAPLALAQTNVTQPGAERSSKGDTTSAASVAS
                     APTGQTVSVNTFIPGKSIAVTPSVASQPVTYMLSENVRYADAAGNTLDPSNIRSGARV
                     RLEVSGTGTTRTVDRIVLVAPQ"
     gene            complement(3858..4595)
                     /locus_tag="VFO30_07250"
     CDS             complement(3858..4595)
                     /locus_tag="VFO30_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET9419120.1"
                     /translation="MKPLTTFFAVFIALGAISMFAQNEAKDQTDKAMQPAQTRLPEDQ
                     PKAATSPSAAATSPSTPASAGQSNQAEMMRQMMEMSKLNENHKLLASLAGTWNYTIRF
                     WMTPDPNAPPQESKGTATTKSMMDGRYFVTDVTGTMKMPGPDGKTRNMQFKGMGVDGY
                     DNVKNKFVASWIDNMGTGIVFSEGTYDPATKSFTYTSEVEPVPGMKSQVREVIRISDK
                     DHRTFEWYENRGGQEVKTMEIAYTRKR"
     gene            4765..5403
                     /locus_tag="VFO30_07255"
     CDS             4765..5403
                     /locus_tag="VFO30_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419121.1"
                     /translation="MSARIDSIPAGSSDDPAINSLIDFAKNGFGDTQMFGLLARRPQL
                     LKRVASVFAYFLAGEGGLIEPRLLELMRVRGAHLNACTYCATVRLQPVAAEVKAKEAA
                     LGVCDISGMTKAQAVTTLQDKLMKSEFTPREAAAISLVDRIVVDPHSVDDTMFAELRK
                     HFSDDEIIELVCASSLFTWAGTLNIVVRLDTDRDGQYRKNLGYATATISAAA"
     gene            complement(5409..6131)
                     /locus_tag="VFO30_07260"
     CDS             complement(5409..6131)
                     /locus_tag="VFO30_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9419122.1"
                     /translation="MNTSDALIAAALRLRQAASKLTFGPPVTHVYNPLEYAWRAHQLY
                     LRCYGSGRKRVLFLGMNPGPFGMAQTGIPFGQIAAVRDWLRIKAAIDKPPNEHPKRRV
                     TGFDCKRSEISGERLWGLFAKRFGTPEKFFAEHFVANCCPLAFIEASGRNRTPDKLPP
                     NERQKLFAICDEHLRDVVRILEPEWLVGVGEFAAERARAIFERSQPRLGRILHPSPSC
                     PESNVDWAGKVTAQLQTLGVWT"
     gene            complement(6142..>6911)
                     /locus_tag="VFO30_07265"
     CDS             complement(6142..>6911)
                     /locus_tag="VFO30_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9419123.1"
                     /translation="SAAFIASGSKVASFVVLGKIVLIGFAPLQGSAEWHAMISGWAPV
                     LAGLAALSILLGNFVALAQTNVRRLLAYSAVAHAGYTLLGIIAADQDGFSATLFYTTT
                     YALTLIGAFGVVGMVRRETGGDDLENFVGLSQRSPLAAACMAVFMLSLAGVPPLAGFF
                     GKFYLFSIAFRAGPNHGLLWLVALALFGSFVSLYYYLIVLKAIFVDQRSEADTDHRLR
                     ITGHLLEKTTTSVLAGLVLLFGIDPSGLVARIAASLQ"
BASE COUNT         1403 a         2012 c         2020 g         1476 t
ORIGIN      
        1 ttatattttt tcgtgatccc agcgaccgtg cgcgtgaccg cgccctggtt gaccagcccc
       61 tgaagctttt cataaacgcg caagcgcagc atctcttcgc cgccgctggc agcgtttcgt
      121 tcccgaagat taagcaggat catgtcgaat aaaggcttga attcgaagga ggccttggac
      181 gaaaggacgg ccacaagctc ttctgtgatg ttgtcgggcg cgcggcgctt gaagctagcg
      241 ccagatgatt tggacattaa tgactatagc cgaaagccgg gaaatagcga gcaggggaca
      301 gaggacagaa gtcaaaagtc agatatacgc ggatccacga gttcgactcg atttttcagc
      361 gggttttgtg ctgaatcggt tagattgaaa gtaaatgagc atggagacga acgaaccgcc
      421 gacgatggac aatctcatct tcggcgaaat ccaggtgctc ctcgcggaga aacgaacttc
      481 gctggctgca ttgcgtaccg ggatcgccgt gttcgctttg cctctgtcgg tcctgagcgt
      541 gctcatcgcg acctcgcgct attacagcat ggaaaaggtg atgcctttgc tcctgccgct
      601 gttgcttctg aacctgggtc tgatcgtgct cggctcctgg ctgatctacc attcgatcca
      661 ccgtattcac cgctacgatc atcgcatccg ggagctcacc cagaaatatc ggtcaatcgc
      721 agagtttatt gactagcacg aaccagaggt ccgtcgcctc cgctgaatct tccgcgagcc
      781 agggaggtca gcgctcagac atcagcaatc agagcgagga cttcagaatc cgccggactt
      841 cggccgttcc ttcaggtgtc tcgttccatt cgccgagtgt ctaaaaagta acagaactgt
      901 tacttttcag cactcgccgc gctcgccccg ggccctacaa ttctttcatg catcacgtgg
      961 cacggcacgc cgcgatttgc agtgaggaac tggcctcgtt catggccagc tcttcgccaa
     1021 gtgcccgcaa cattttcccc gcatcggtgg tttatttaat cgccgacacg ttctctccag
     1081 agcagcaacc cagttaaaaa aattcttatg aaaacaaact atcgtgccgc gggcgtgccc
     1141 atgcttgcgg ctcttatctc cctcgcagtg acatcgtatg cagctgccca aagtggcggc
     1201 ttcgtacaga caaacctcgt ctccgacatt cccggagtgg cagagcgagt cgattcgaac
     1261 ctggtaaatc cgtggggcat tgtcgcctca cccagtaaca ccatctggat agccgacaac
     1321 ggcactggcg tgtcgaccct ctatagtccg accggcgcca agcagccttc cggcaatccg
     1381 ctcgtcgtca cgatcccggc atcgaagaca agcaccgaag gcggcaatcc cactggcatt
     1441 gtgttcaacg gcgccggcgg tttcgtcgtc accgaagaca ctcgctcagg gtcgagcttc
     1501 ttcatcttcg ccagcgaaga tggcgtgatc tcgggctgga acccgcaggt tgcgctcgat
     1561 catgccgtgg tggcggttga tcacgggaag cgcgaagcca tttacaaggg cgcggcacta
     1621 gggacaacgg ccggcggctt gcggctctac gtgaccaatt tccacgccgg cacggtggag
     1681 atttacgacc agaatttctc gcgcattcac ggcccaacta aattcgtcga tccgactcta
     1741 cccagcggct tcgctccctt cgggattgcg aacatcaatg gctcgatcta cgtcacctac
     1801 gccaagcagg acgcagacgc ggaagatgat gtggccgggc cgggcctcgg cttcgtgaac
     1861 gtcttcgata cccagggcaa tttcatcaaa cggctgatct cgcgcggcaa gctgaatgca
     1921 ccctggggtc tggcgctcgc tccgagtggc ttcggctcct tcggtagcgc gttgttggtt
     1981 ggcaactttg gcaatggcca catcaatgcc tatgatccga acagcggggc tttcctcggc
     2041 cagttaacaa aggcgggcga tggcagatcg ctggtgatcg acggtttgtg gggcctcttt
     2101 ttcatcgaca aacgcctgtt ctttactgct ggcattgccg atgaagctca cggtttgttt
     2161 ggtgtgatta aggcgagcca ataacacgcg gaaaatcgca cggatctggg cgctcgtttg
     2221 cgagtgctga cctgccgtgg atgctcgctt gcgctcggaa attacgccag caggcgagcg
     2281 gcctatcggc cgagtaaagc gggacgaggc agtgatgagt gatatgtgac gagtgacgag
     2341 ccggcgaacc agccaaacgg attcaccacg gaatgacttt gcggtcgcgc cagaacttgc
     2401 cggtttcctt ttgcggagcg tcgccggcga gccagatccg cctccgccaa ggctacggcg
     2461 tgacaggcga ttccgacggt ggtgttacca aggaattatt ttgcggtccc tgaagaactt
     2521 accagtcatc gagtgcggag cttcagatac tagccacacc atcccggcgg cgccttcagc
     2581 gactgaacgc gtcgcgttag cgccgcccat gtcggtgcgg acccagccgg gacagacgga
     2641 attgaccgcg aattttggca gggccgctgc gagctgagag gtgacgccgt ttaatgccgt
     2701 tttcgaaatg caataggccg gcgcccaacc gtcggcgcca tcagccagct gaccgccgct
     2761 gctggagaca ttcacgatac gcgccgcgtc gctctttttc aggaacggca cgaaggcctg
     2821 cgtgaccagc agcgcgccga gcgcgttcgt gcgcaacgtc ccttcgaata tctcgggcgt
     2881 gattgtgagc acatccttgt catcatcgag cagaatgccg gcgttgttca ccagcgcgtc
     2941 gagccggtcg ctgtggcgag aaaattcttc cgccgcgcgc ttgacactct ccggtttgga
     3001 gatgtcgagc ttgatgaacg taacctcgcc gtagctttgc ttctcacctt cgcggtgaag
     3061 tttcgcaaca gctgcttccc ccgcgtcttc accacgcgcg ccgagaaata cgcgaaaacc
     3121 tttgcgcgcc agcagccgca cgacttcaaa cccgattcct ttgttcgcgc cggtgacaag
     3181 cgcgatgcgt tgctcttttg tcatgccgca gcctgcggtg cgcaacccga aaaagcaaaa
     3241 cgcccgcggg ccgcgagcgt ccgctcctcg tgccctccgt taccgaaaac tcaaacgcct
     3301 acgtttattg cggcgcgacg agaacgatgc gatccaccgt gcgagttgtt ccggtaccgc
     3361 tgacttcgag ccgcacgcgt gcgccggagc ggatgttgct cggatcgagt gtgttccccg
     3421 ccgcatcagc gtagcgaaca ttctccgaga gcatgtatgt gaccggttgc gaagcgacac
     3481 tcggcgtgac ggcgatagat ttgcccggta tgaacgtatt gaccgagaca gtctggccag
     3541 tcggtgcgga agcaaccgac gcagcggacg tggtatctcc tttgctcgat cgttctgcgc
     3601 cgggctgggt aacgtttgtc tgcgccagag cgagcggcgc gatcgtgatg caagcaagag
     3661 caatcatttt ctctttcata tgggtccctt tcgttttgag gtttgagttt cctcttagtt
     3721 acgcgccccg gtcattgttc ggatgttcaa gtgcggccaa caaccacccg ctaccgtgat
     3781 ttatctgtgg tggttgccgc tacaacttcc agtctctctt ccgcctccgc tgggctacgg
     3841 cgcgaccggc catcgtttta ccgtttacgc gtatacgcga tctccatcgt cttaacttcc
     3901 tggccgccgc ggttctcgta ccattcgaac gtgcgatggt ccttgtcgct gatccttata
     3961 acttcgcgga cctgtgattt catgccgggc acaggctcga cttcggacgt gtaggtgaat
     4021 gatttcgtag cgggatcgta ggtgccttcc gagaacacga tgccagtgcc catgttgtcg
     4081 atccacgagg cgacgaattt gttcttcacg ttgtcatagc cgtcgacgcc catgcctttg
     4141 aactgcatat ttctcgtttt cccgtccgga cccggcattt tcatggtgcc ggtcacgtcg
     4201 gtgacaaaat agcggccgtc catcatcgac ttggtcgtgg cggtgccttt cgactcctgc
     4261 ggcggcgcgt tcggatcggg agtcatccag aatctgatcg tgtaattcca agtgccggcg
     4321 aggctggcga gcagtttgtg gttttcgttc agtttcgaca tctccatcat ctgcctcatc
     4381 atctcggctt ggttcgattg gccagctgaa gcaggcgtgc tcgggctggt tgccgcggct
     4441 gacggcgaag ttgccgcttt cggttgatct tccggcaacc tggtctgcgc cggttgcatc
     4501 gctttgtcgg tctgatcttt ggcttcgttc tgcgcaaaca ttgaaattgc gccgagcgcg
     4561 atgaatacag caaagaaggt ggtcaacggt ttcatggcgg cgattttgcc agtgacaagt
     4621 gaccggagac aagtggcaag tggcggcatc cggctttccg caatccgata tccagcatcc
     4681 gcaagccaac gctccaattg tcttgaattg ctacaatgcg ccggcgcaga attcagcgaa
     4741 tcttaagcaa caaaaggagg ccacatgtct gcccgtattg attccatccc ggcggggtct
     4801 tccgatgatc ctgccattaa ttcactgatc gatttcgcca agaacggttt cggcgacacg
     4861 caaatgttcg gtctgctcgc gcggcgcccc caactgctca agcgcgtcgc gtcggtcttc
     4921 gcctattttc tcgcgggtga aggaggtttg atcgagccgc gcttgctcga gttgatgcgg
     4981 gtgcgcggcg cgcacctgaa cgcgtgcacg tattgcgcga cggtccgact tcagccggtg
     5041 gcggcagagg taaaggcgaa ggaggccgcg ctcggcgtct gcgacatcag cgggatgacg
     5101 aaagcgcaag cggtcacgac gctgcaggac aagctgatga aaagcgagtt cacgccgcgc
     5161 gaggcagctg cgatttcgct cgtcgaccgc atcgtggtcg atccgcacag cgtcgacgac
     5221 acgatgttcg ccgagctgcg caagcatttc agtgacgacg agatcatcga gctggtctgc
     5281 gcgagcagtc ttttcacctg ggccggaaca ctgaacatcg tagtgcggct ggatacggat
     5341 cgcgacggcc aatatcggaa gaatctcggt tacgcaacgg cgacgatctc cgcagcggcg
     5401 taaccgcgct acgtccagac gcccaacgtc tgcaattgcg ccgtcacttt gccggcccaa
     5461 tcgacattcg attccggaca cgatgggctc ggatgcagga tgcggccaag gcgcggctga
     5521 gatctctcga agatcgccct cgcgcgttcc gcggcgaact cgcctacgcc gacgagccat
     5581 tcaggttcga ggatgcgcac gacatcgcgg agatgttcgt cgcagatcgc gaacagtttc
     5641 tgacgttcgt taggcgggag tttgtccgga gtgcgattcc taccgcttgc ttcgatgaat
     5701 gcgagggggc aacaattcgc gacgaagtgc tcggcgaaga atttttcagg cgtgccgaat
     5761 cgcttcgcga ataagcccca caagcgttcg ccgctgatct cgctgcgttt gcaatcgaac
     5821 ccggtgacgc gccgcttcgg atgttcgtta ggcggcttgt cgatcgccgc ttttattcgg
     5881 agccagtcgc gcactgctgc gatctgcccg aaaggaatgc cggtctgtgc catgccgaac
     5941 ggtcctggat tcatgccgag aaacagcacc cgttttcggc cggagccgta acaccgcaga
     6001 taaagctggt gcgcgcgcca ggcgtactcg agcgggttgt aaacgtgcgt gaccggtgga
     6061 ccgaacgtga gtttgctcgc ggcctggcgc agccgcagcg cagcggcaat caatgcgtcg
     6121 cttgtattca agctgcgtcg actactggag tgaagcggcg attcgtgcga ctaaaccgga
     6181 cggatcgatc ccgaagagaa gaacaagccc ggcgagaacc gaggtcgtgg tcttctcgag
     6241 taagtggccg gtgatccgta atcggtgatc ggtatcagct tcgctccgct ggtcaacgaa
     6301 gatggccttc agcacgatga ggtagtagta gagcgaaaca aagctgccga agagtgcgag
     6361 cgcgacgagc cagagaagcc cgtgattggg tccagcacga aacgcgatac tgaaaaggta
     6421 aaatttgcca aaaaatccgg ccagcggcgg gaccccggcg agagacaaca tgaaaacggc
     6481 catgcacgct gcagctaatg gcgagcgttg gctaaggccg acaaaatttt caagatcatc
     6541 gccgcccgtc tcgcggcgca ccataccaac gacgccaaac gcgccgatca gcgtcagcgc
     6601 gtaagtggtt gtgtaaaaca gggtcgcgga gaatccgtcc tggtcggccg cgatgattcc
     6661 gagcaaagta tagccggcgt gcgcgaccgc agaataggcg agcaggcgac ggacattggt
     6721 ctgcgcgagc gcaacgaaat tgcctaataa gatcgacaat gcggcaagcc ccgcgagtac
     6781 cggcgcccat ccggagatca tcgcgtgcca ctcggcgctc ccctgcagcg gcgcgaaacc
     6841 aatgagcacg atttttccga gcacgacaaa ggaagcgacc ttcgagcccg atgcgatgaa
     6901 agcggcgctg g
//
LOCUS       DASTDG010000220         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_877668, whole genome shotgun sequence.
ACCESSION   DASTDG010000220 DASTDG010000000
VERSION     DASTDG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877668"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFO30_07270"
     CDS             <1..459
                     /locus_tag="VFO30_07270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET9419124.1"
                     /translation="AMHPSRPSTLFMQKHWDVMRSDDAGDSWREVSGNLPSDFGFPID
                     VHAHEPETIYVVPIKSDSEHYPPDGKLRVYRSRTGGNEWEELTKGLPQKDCYVNVLRD
                     AMSVDSLDPCGIYFGTTGGQVYASADSGDSWLPIVRDLPAILSVEAQTLP"
     gene            536..832
                     /locus_tag="VFO30_07275"
     CDS             536..832
                     /locus_tag="VFO30_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9419125.1"
                     /translation="MIRVILPHHLRTLAKVGDEVHVDLNGSTTVSAILDALEEKYPML
                     RGTIRDHDTHKRRPLVRFFACGEDISHESPETPLPDLIAKGAEPFFIMGAIAGG"
     gene            926..1738
                     /locus_tag="VFO30_07280"
     CDS             926..1738
                     /locus_tag="VFO30_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET9419126.1"
                     /translation="MQRLIIRLFIVAFLIVELSRTLYANPPSDPKAGSVTLDAKPEPI
                     AIDPARTAVIVVDMENDFAAKDGMFDRAGADISGAQKAIAPTAKALAAARQAGLKIVY
                     LKMGYHADLSDLGASDSVNRTRHLKFGVGQTIRSPDGRESRILIRDTWDTDIVPELKP
                     QPGDVVMYKTRFSGFYQTDLDATLKKFGIKYLIVTGVTTSICVESTVRDAMFRDYLCV
                     LLSDCMSEPIGHDLPRTNHEASLLNTEVLLGWVSDSEGFIKALSAKTAQASR"
     gene            1735..2163
                     /locus_tag="VFO30_07285"
     CDS             1735..2163
                     /locus_tag="VFO30_07285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9419127.1"
                     /translation="MSKTFESSRAPEPVGAFPHAKRAGNLLFLSGIGPRVRGSKEIPG
                     VTLDASGKIDNYDIEKQCRAVFENVRLVLEDAGASWNDIVDATVFLTNMKKDFPIYNK
                     LYAEYFAGPGKPNPTRTTVEVTALPTPIAIELKVIAAVEK"
     gene            2243..2725
                     /locus_tag="VFO30_07290"
     CDS             2243..2725
                     /locus_tag="VFO30_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9419128.1"
                     /translation="MSTSRSFRIIAADHTGITVSNLECSLRLWRDVLGFELSHTPHHT
                     GKLASEVTGVPGAEISIAVLKGYGHKIELLEYLAPADRKRHVDLRPCDVGHVHVALLV
                     DDLDAVLEKLAASEWKPAGKAQTLTAGPNAGKRVVYVRDPDGATIELMQPPADSPVER
                     "
     gene            complement(2744..>3259)
                     /locus_tag="VFO30_07295"
     CDS             complement(2744..>3259)
                     /locus_tag="VFO30_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9419129.1"
                     /translation="DDLKLMAERGTYLDPQAGLVIENYFANKERFLGTPGYTEEGFAA
                     MERILSIDRDFVRRAVKIPGLKVVFGTDALAGAHGRNAEEFIDRVRDAGVDPMSAMVS
                     ASSLAAEAMRMSDSIGSIAPGYDADIIALDGDPLKDITAVRRVVFVMKGGVVYKNVPR
                     GAIPNNADIQP"
BASE COUNT          749 a          889 c          939 g          682 t
ORIGIN      
        1 gccatgcatc cgtcgcggcc gagcacgctc ttcatgcaaa aacactggga cgtaatgcgc
       61 agcgatgacg ccggcgattc gtggcgcgag gtgagcggca atttgccgag cgatttcggt
      121 tttccgatcg acgtgcacgc gcatgagccg gaaacgattt atgtcgtgcc gatcaagagc
      181 gactctgaac attatccgcc tgacggcaag ctgcgcgttt accggagtcg caccggcgga
      241 aacgaatggg aagaactgac caaaggtctg ccgcaaaagg attgttacgt aaacgtgctg
      301 cgtgacgcaa tgtcagtcga ttcgttagat ccgtgtggga tttatttcgg aacaaccggc
      361 gggcaggtgt acgcgtccgc tgattcgggc gattcttggc tgccgatcgt gcgcgatctt
      421 ccagctattc tttcggtcga agcgcaaacg ttgccatgac agaacttcaa tccaacgttt
      481 cgttgaaact tgacggcttt aagccgtcaa gtttgccggg gcaagttgtc gagaaatgat
      541 tcgcgtaatt cttccgcatc atctgcgcac gcttgcgaaa gtcggcgacg aagtgcatgt
      601 cgatcttaac gggtcgacaa cagtcagcgc gatcctcgac gcactggaag aaaaatatcc
      661 aatgctgcgc ggcacaattc gcgatcacga cacacacaaa cgccggccgc tcgtgcgctt
      721 tttcgcctgt ggcgaagata tttcgcacga atcgcccgag actcccctac ccgatctgat
      781 cgctaaaggc gccgagccat tcttcatcat gggcgcgatc gcgggaggct aaaacgaagg
      841 ctgaaggaag aaggatgaat tatgaaagga agaacgcaga cttcttgatt tcatcgagca
      901 gcggaagccg taacatcttt gcatcatgca aaggttgatc atcagactgt ttatcgtcgc
      961 gtttctcata gttgagttga gtcgaaccct gtacgcgaat ccaccttccg acccgaaggc
     1021 tggatcggtc acacttgacg caaaaccgga accgatcgcg atcgatcccg cgcgaactgc
     1081 ggtcatcgtt gtggacatgg agaacgattt cgcggcgaag gacggaatgt tcgatagagc
     1141 tggagccgat atttccggcg cgcaaaaagc catcgctcca acggcaaaag cgctcgcggc
     1201 ggcgcgccag gccggactaa aaatcgttta cctgaagatg ggataccacg ctgacctttc
     1261 ggacttgggc gcttctgatt cggtaaaccg cacgcggcat ttgaaatttg gcgtcggtca
     1321 gacgattcgc tcgcccgacg gtcgcgaaag ccgaatcttg atccgagaca catgggacac
     1381 ggacatcgtt ccagagctca agccgcaacc cggagatgtc gtgatgtaca aaacgcgctt
     1441 cagcgggttc taccaaaccg atttggacgc aacgctgaag aaattcggaa tcaaatatct
     1501 gatcgtcacg ggcgtaacga cgagcatttg cgtggagtcg accgtgagag acgcgatgtt
     1561 cagagattat ctttgtgtct tgctaagcga ttgcatgagc gagcccatcg gccacgattt
     1621 gccgagaacc aaccacgaag cctcgctttt gaataccgaa gtcttgctcg gctgggtttc
     1681 ggactcagag ggattcatca aagccttgtc cgcgaaaacg gcgcaagcga gtcgatgagc
     1741 aaaacgttcg agagctcgcg cgcgccggag ccggtgggcg cgtttccgca cgcaaaacgg
     1801 gccggcaatt tactttttct ttcgggaatc ggaccgcgcg tgcgcggcag caaagaaatt
     1861 cccggcgtca cactcgatgc ttctggcaag atcgacaatt acgatatcga gaaacaatgc
     1921 cgcgcggtgt ttgaaaatgt ccggctcgtg ctggaagacg ccggcgcgag ctggaacgac
     1981 attgtcgatg ccacggtctt cctcacgaac atgaaaaaag acttcccgat ctacaacaaa
     2041 ctttacgccg agtattttgc gggtcccggc aagccaaacc cgacacggac gacggttgaa
     2101 gtgacggcct tgccgacgcc gatcgcgatt gagcttaaag taatcgcggc ggtggagaag
     2161 taatcatcca cagattccgc agattatctt ctgaattcgg cgttggacgt tgagcgttgg
     2221 gggttggacg tttctttggg gaatgagcac gagtaggtcg tttcgaatca tcgcggccga
     2281 tcacaccggc atcaccgttt caaatctcga gtgttcctta cgtttgtggc gcgatgttct
     2341 gggattcgag ctgtcacata caccgcacca taccggcaag ctcgcgagcg aagtgaccgg
     2401 agttccgggt gcggaaatct ccattgcggt gttaaaaggc tatggacaca agatcgagct
     2461 gctcgaatat ctcgcgccag ctgatcgaaa acgacatgtc gatcttcgac cgtgcgatgt
     2521 cgggcatgtg cacgtcgcgc tcttagtcga tgatctcgat gcagtcttgg agaaacttgc
     2581 tgcgtcagag tggaagccgg ccggtaaagc gcaaacgttg acggctgggc caaatgccgg
     2641 caaacgcgtc gtctatgtgc gcgacccgga cggcgcgacc atcgagctca tgcagccgcc
     2701 cgccgactca ccagttgagc gatgagccca agttgcgcat tgctcacggc tggatgtccg
     2761 cattgttggg gatcgcgccg cgcgggacgt tcttgtaaac cacgccgcct ttcatgacga
     2821 aaacgacgcg gcgcactgcg gtaatgtctt tgaggggatc gccatcgagc gcgatgatgt
     2881 cggcgtcgta gcccggtgcg atagagccga tgctgtcgct catgcgcatg gcttcagcgg
     2941 cgagcgagct cgcagatacc attgcgctca tcgggtcaac tccagcatca cgcacgcgat
     3001 caatgaactc ctcggcgtta cggccatgcg cacccgcaag cgcgtccgta ccgaagacaa
     3061 ctttgagtcc gggaattttg acagcgcggc ggacgaaatc gcggtcaatt gacaagatcc
     3121 gctccatcgc cgcgaaaccc tcctcggtgt atccgggcgt accgaggaag cgctccttgt
     3181 tcgcgaagta gttctcgatc acgagtcccg cttgcggatc gagataggtg ccgcgctcgg
     3241 ccatcagctt gagatcatc
//
LOCUS       DASTDG010000221         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1122721, whole genome shotgun sequence.
ACCESSION   DASTDG010000221 DASTDG010000000
VERSION     DASTDG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122721"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFO30_07300"
     CDS             complement(<1..482)
                     /locus_tag="VFO30_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419130.1"
                     /translation="MRIFNRAHAPWFALVLGATLFATWIYIGNFAPERLAPQFRPPPQ
                     FIQTPSEHRSVGGTPLGLIFGAISLAIFVFAALLGVRKKIVLWRIGNIQRWLRAHIWL
                     TLLTIPLVLLHSGFRLGGPMTTLLMFLFAVVIVSGIYGLVLQHVMPRLMKERLPAETV
                     "
     gene            complement(588..2270)
                     /locus_tag="VFO30_07305"
     CDS             complement(588..2270)
                     /locus_tag="VFO30_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9419131.1"
                     /translation="MPDVVEKLTGGEPLSVDQLLEYLPALKELPRAQLDNFAKGAAAL
                     HRYRAGEIVCEEGEFGSTAYYIVSGTVDIFISNPLAHLRTRPARNFWDRSLRRMRSFL
                     TSDLEDRRAEAHQRKFIGIDADVDLPVQRPLAQLGPGDLFGEMTCRTYQPRSATVRTR
                     EPCVMIEMLRVILDMLLGNRQPSELTKATTKVKMPTFKGTSFKAELDKKYRERSLDNH
                     LRSVPLFATLDDEFIGYLKQKVELLSYNQGQVICREGDDADAFYLIRSGVVRVSQALP
                     GGEIVRTYLSRGDWFGEIGLLRKMKRTATCSALDAVDVVKIPEAEFDLMLEKFPDVRA
                     QLEPVAAARLAAADKRRLPPAGFRLDDFLNQGLFEAQNLLLIDLDNCTRCDACVRACA
                     TAHDGVSRLLRDGLRYDHYLVATACRSCRDPLCMTQCPVGSIRRKENLEIIIEDWCIG
                     CGKCAELCPYGNINMHPLETIREIRTEAKSPTPGAPSTVKIEKKKMTTYKANTCDLCT
                     QLSVPSCVYACPHDAAKRVDPQRFFADARVTQDQLLQSAWSPARRYKTHHTE"
     gene            complement(2378..>2560)
                     /locus_tag="VFO30_07310"
     CDS             complement(2378..>2560)
                     /locus_tag="VFO30_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419132.1"
                     /translation="RGQILKQLVDTERFAAGQFFNHVRHGTSREEISRRKLLWKNFPG
                     SAAHDQGSNPSPQSSP"
BASE COUNT          545 a          719 c          754 g          542 t
ORIGIN      
        1 taaactgttt cggctggtaa tcgctctttc atcagccgcg gcatcacgtg ctgaagcaca
       61 agtccgtaaa tgccgctgac gatcacgacg gcaaaaagaa acatcagtaa cgtcgtcatc
      121 ggcccgccga ggcgaaaccc gctatgcaag agcacgagcg ggattgtgag caacgtgagc
      181 cagatgtgtg cgcgcaacca ccgttgaatg tttccaatgc gccagagcac aatctttttg
      241 cgaacaccga gcagggccgc gaacacgaaa attgccaatg aaattgcacc gaagatcagg
      301 ccgagcggcg tgccgccgac gctgcggtgt tccgagggcg tttggatgaa ctgcggcggt
      361 gggcgaaact gcggcgccaa acgttcggga gcaaaattcc cgatgtagat ccacgtggcg
      421 aacagcgtcg cgccgaggac gagagcaaac cagggagcgt gagcgcgatt aaaaatgcgc
      481 atccccggaa tttaagattt cagattggaa attgacgatt gaattcgtcc gacgcgcgca
      541 accatcgcct caatctgcga tcgccaattt gcaatctgca atccggttca ctcggtgtga
      601 tgcgttttgt accggcgcgc cggtgaccac gcgctctgta atagctgatc ctgcgtgacg
      661 cgcgcgtcgg cgaaaaagcg ctgcggatcg acgcgtttgg ccgcgtcgtg cgggcaagca
      721 taaacgcagc tcggcacgga aagctgtgta cacaggtcgc acgtgttcgc tttataggtg
      781 gtcatctttt tcttctcgat ctttaccgtc gacggagcac ctggagttgg tgatttcgcc
      841 tccgtccgga tttcgcgaat ggtttcgagt ggatgcatgt tgatattgcc gtacgggcaa
      901 agctcggcgc atttcccgca gccgatgcac cagtcttcga tgatgatctc gaggttttct
      961 ttccggcgaa tggatccgac cgggcattgc gtcatgcaaa gcgggtcgcg acaagatcga
     1021 catgctgtcg cgaccaggta gtgatcataa cgcaaaccgt cgcgcaacag acgcgaaacg
     1081 ccgtcgtgcg cggtcgcgca ggcgcggacg cacgcatcac aacgtgtgca gttgtccaga
     1141 tcgatgagga gaaggttttg cgcctcgaat aatccttgat tcaaaaaatc gtcgagccgg
     1201 aacccggctg gcggcaggcg gcgtttgtcc gcggcggcca gccgcgctgc tgccactggt
     1261 tcgagttgcg cgcggacgtc aggaaatttt tccagcatga gatcgaactc ggcctcggga
     1321 attttgacca cgtcgaccgc atcgagcgcc gagcaagtcg cggtgcgttt cattttccgc
     1381 aacagaccga tttcgccaaa ccaatcgccg cggctgagat aagtccgaac gatttcgccg
     1441 cccggcaatg cttgcgaaac ccgcaccaca cccgagcgga tcagataaaa tgcgtcggca
     1501 tcatcaccct cgcggcagat aacctggccc tgattatagg agaggagctc cactttctgt
     1561 ttgaggtagc cgatgaactc atcgtcgagc gtagcgaata gcggaacact gcgcaggtgg
     1621 ttgtcgagcg agcgctcgcg atattttttg tccagctcag ctttgaacga agtgcctttg
     1681 aatgtcggca tcttcacttt cgtagtcgcc tttgttagtt ccgacggctg ccggttgccc
     1741 agcagcatgt cgaggatgac gcggagcatc tcgatcatca cgcacggctc gcgtgtgcgc
     1801 acggtcgcag agcgcggttg atacgttcgg caggtcattt cgccaaacag atcgccgggc
     1861 ccgagctggg cgagcggacg ctgcaccgga agatcgacat ctgcatcaat gccgatgaat
     1921 tttcgttgat gcgcttccgc gcgccggtcc tcgagatcgc tggtaaggaa gcttctcatc
     1981 cgccgcaggc tgcggtccca aaaatttctc gcgggccggg tgcgcaaatg cgcgagcgga
     2041 ttgctgatga aaatgtcgac cgtgccggag acgatgtagt aggcggtcga accgaactcc
     2101 ccctcctcgc aaacgatctc accggcgcga tagcgatgta aggccgccgc acctttggca
     2161 aagttgtcga gctgcgcgcg tggcaactct tttagcgcgg gcagatactc aagcaactgg
     2221 tcgacactga gcggttcgcc gccggtcaat ttttcaacca cgtcaggcat ggcacctcgc
     2281 gggaagaaat atcccgtcag gggttgttat gaaagaattt tccccgcgtg cttgtcacgc
     2341 tcaaccctca cctggcgctg atgcgtcctc ctttccctca gggagaggat tgaggtgagg
     2401 gattgctgcc ttgatcatga gctgcgcttc cgggaaagtt cttccataac aatttgcggc
     2461 gcgatatttc ttcccgcgag gtgccatgcc tgacgtggtt gaaaaattga ccggcggcga
     2521 accgctcagt gtcgaccagt tgcttgagta tctgcccgcg
//
LOCUS       DASTDG010000222         6486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1112436, whole genome shotgun sequence.
ACCESSION   DASTDG010000222 DASTDG010000000
VERSION     DASTDG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112436"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFO30_07315"
     CDS             <1..439
                     /locus_tag="VFO30_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9419133.1"
                     /translation="KSVMGRTEDGRKLPLHPLWHRRWSTKANSVSYPVQRGAEALYSP
                     EGRKQVRALIDHYMGNARILHDGCRSASIQVFGGKNAPYLWAKTPGGLTSWQLFDRML
                     HELNIVVTPGSGFGAQGEGYFRISAFNSRENAEEASRRLATFR"
     gene            508..981
                     /locus_tag="VFO30_07320"
     CDS             508..981
                     /locus_tag="VFO30_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419134.1"
                     /translation="MRANLPQLYRFCFLMLGDAEKAEEVFEATLREAAQRAATGEAPA
                     DRCWLFREARGRCLEVSERGLQAEDVDHAQDELSAAAPDQIRRLDVAQLAIWISGAPE
                     PQRTALALFYLGEFCRADILKIAELKTHELADLLSLARGQFQAWLDASFPLERTS"
     gene            978..1754
                     /locus_tag="VFO30_07325"
     CDS             978..1754
                     /locus_tag="VFO30_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419135.1"
                     /translation="MKTARLSRRQQLLIACAPVNEQPIADRNMRAAVFRARDSEEFKI
                     QQSFDRAVVDLVQTVPVPSAVAEWLSSEKAIEQPKWTWKRAAKNPAVVAVGIAVLVIA
                     AVFAIQFAEHLHDFPGSAHARKLLSVARSPRSVLLDPVRTEAGALSDLFFMKHNLEHY
                     EVPPEFADFQTLGCRVFDDEEAGRVAQIWVVEKHMQFFLFPAERDTKTGIAPDFSNWR
                     FVEQEGWTGAVQQRHGVCFMAAVRGDATQLAPYLNKLHSH"
     gene            1816..1887
                     /locus_tag="VFO30_07330"
     tRNA            1816..1887
                     /locus_tag="VFO30_07330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Met,seq:cat)
     gene            1976..3502
                     /locus_tag="VFO30_07335"
     CDS             1976..3502
                     /locus_tag="VFO30_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET9419136.1"
                     /translation="MNDRNAVEKLFARGNSYWLTRFVILRLLGFVYAIAFFIAAKQLV
                     PLIGDHGLTPATHFLSAIEAQLGSRSAGTLHLPTLFWFGLSDRGLSMFAWIGFALSLV
                     VLGGYANAILLTLLWSMYMSIVHVGQIWYGYGWEIQLLETGFLSIFLCPLIDGRPFPK
                     CRPPILIIWLFRWLGFRIMVGAGLIKLRGDACWRDLTCLYYHYETQPIPNPISRYLHF
                     APHWFHKLGTAWNHCIELIVPWFSFYPRLARHIAGVLLISFQIILIISGNLSFLNYVT
                     IIPFLACFDDTLLSRILPKVLVRQAKRRAAESQTSHINNRIAVALSILVAYLSVAPVI
                     NMASGQQLMNYSFNPLDLVNTYGAFGTVGRERDEIIFEGTDDAVITGDTKWKEYEFLA
                     KPGDPNRRPPFVAPYQPRIDWQIWFAAMSSPAEYPWTLHFVWKLLHNDRGTLSLIANN
                     PFPDAPPRYIRARLFRYHFAPLGEKAWWQREPIGEWLPALSPDDAEFRRLLAAMDWLD
                     "
     gene            3513..4424
                     /locus_tag="VFO30_07340"
     CDS             3513..4424
                     /locus_tag="VFO30_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9419137.1"
                     /translation="MEVAILGDSVCTGVYISSPWSTFWRARRARAGNWFVDTTPPARG
                     IQSVCKRLEELTPVVATECAGIGALVDGEVERQNFFRRILRTRDFSGQIRQLLRAPRL
                     PDLILISIGHNNIDWTWRRTARELERPDVILQELSVRFREDFARHLQRLLNVASAQSH
                     RVAIVVYGLVNFESYFKARAVAEQLHAQDRTRYPHLETTYKYFVSFRPLYRSHLVRLA
                     RMLNEQLRTIVTDLNCDLAEHRNVQLRYSDALATADLSRIELLHAVDGWHPSAAGHNV
                     LASAAFDDLAPTLEFLGVNSPSTAARH"
     gene            complement(4486..6207)
                     /locus_tag="VFO30_07345"
     CDS             complement(4486..6207)
                     /locus_tag="VFO30_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9419138.1"
                     /translation="MKLLFGCVARVHVVRVETANREGGFLLAANHISHFDPFIISAVV
                     RRKIDWMAMAEFFPVPIIGLFLRAVDAFPAARNRADRATIRTAIERLKAGRIVGVFPE
                     GGIRNGACSLLEGAPLRAGASTLAHMAGVPVVPCVILGSDRLYNLKRWLPLRRTPVWI
                     AFGECVPSFPQLDKSVGRARIEREVTAAFQKLYTELREEFHLTPDDLPHSPQERMRGS
                     SASASLAAHKPGNRSGSPIIRAAPWRRFAATSVDFAMCASMNLLQSRHRLDTRSREEM
                     ERYVADCEKLSVHEYYYAQDDVDVAAALDNGTTPRILTWRSPIQTRFPANDMARVDLF
                     PSKRGWNATPTVLMLHALLSATHFGYRRLARSFNERGWNACFVHLPYHYSRVPRGYWN
                     GELAITADLIRNAEGLRQGVMEVRQLLRALRKMGCSEVGILGTSYGGWIGALLAAVER
                     DLRFVALMAPIVNVEHAIWQSPGTASMRKELRRMSIEPALVARHFHLSSPTHIQPACD
                     PGRVLFVSGEFDTIARPEDLAEIHHRWRGSQLLRVRQGHFGYRMLRETVVHLAQHDLL
                     APNNLTA"
     gene            complement(6270..>6486)
                     /locus_tag="VFO30_07350"
     CDS             complement(6270..>6486)
                     /locus_tag="VFO30_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419139.1"
                     /translation="IAVMGVQDEAKGEALVLLAAIDVDLAQLREKLREAGVPNLWIPK
                     KIWRVDAIPVLASGKLDLRKCQELAAP"
BASE COUNT         1306 a         1905 c         1871 g         1404 t
ORIGIN      
        1 gaaatccgtt atgggtcgaa cagaggacgg gcgcaaactg ccgctgcatc cgctgtggca
       61 ccggcgttgg agcaccaaag cgaacagcgt gagttatccg gtccaacgcg gggctgaggc
      121 tctgtattcg cccgagggcc ggaaacaggt tcgcgcttta atcgatcact acatgggcaa
      181 tgcgcgaatc ctgcacgacg gctgccggag cgccagcatc caggtgttcg gcggcaaaaa
      241 tgcgccgtat ctttgggcga aaacacctgg tggcctaacg agttggcaac tattcgaccg
      301 aatgctgcac gaactgaata ttgtcgtgac gcccggcagc ggatttggtg cgcaaggcga
      361 gggttacttt cgcatttctg ctttcaatag ccgcgagaac gctgaagaag cgtcccgccg
      421 gctcgccacc ttccgctaag atagcggctt gtcgattcag acgagtcgcg taacgtccag
      481 cgaaaatttt tgccgaggcg cgtcggattg agagctaatc ttccgcaact gtatcgcttt
      541 tgttttctga tgctcggcga tgcagagaaa gcggaggagg tctttgaggc gacgctgcgc
      601 gaggcggcgc agcgtgcggc aacgggcgaa gctcccgccg accggtgctg gctctttcgc
      661 gaagcgcgcg gccgttgtct ggaggtgagc gaacgaggcc tgcaggcgga agatgtggat
      721 catgcacagg atgaactctc agctgcggcg cccgaccaga tccgccggct cgatgtagcg
      781 cagctcgcga tttggatctc gggagcgccg gagccacagc gtaccgcgct cgctctattt
      841 tacctcgggg aattctgtcg cgcggacatt ttaaagatcg ccgagctgaa gacgcacgaa
      901 ctggcggacc tccttagttt ggcgcggggt caatttcagg cgtggctgga tgcgagcttc
      961 ccgctcgagc gaacgtcatg aaaacggcgc gcctgtctcg gcggcagcaa ctcttgatcg
     1021 cctgcgcgcc cgtcaacgag cagccaattg ctgatcgtaa catgcgcgcg gccgtgtttc
     1081 gcgcccgcga ttcggaagag tttaaaatcc agcaaagctt cgaccgcgcg gttgtggatc
     1141 ttgtccagac tgttccggtt ccctccgctg tagcggaatg gctctcgagc gaaaaagcga
     1201 tcgagcaacc gaagtggact tggaaacgcg cggcgaagaa tcccgccgta gtcgcagtcg
     1261 gaattgcggt ccttgtcatt gcggcggtgt tcgcgatcca attcgccgag cacctgcacg
     1321 attttcccgg ctcagcgcac gctcgaaaac tgttatcggt ggcgagatcg ccccgctcgg
     1381 ttttgctcga cccggtgcgc accgaggccg gcgcgttgag cgatttattt tttatgaagc
     1441 acaacctcga gcattacgag gtgccgcccg agtttgcaga ttttcaaact cttggctgtc
     1501 gcgtcttcga cgacgaagag gctgggcgcg tcgcgcagat ctgggttgtc gaaaaacaca
     1561 tgcagttttt tctgttcccc gctgaacgcg acacgaagac tggcattgcg cccgattttt
     1621 ccaactggcg ttttgtggag caggagggtt ggaccggcgc agtgcaacag cgtcacggag
     1681 tttgtttcat ggccgccgtg cgcggtgacg ccacacaact cgcgccttac ctaaacaaat
     1741 tgcattcgca ctgaggtaag ccttagccgg caccgttgac acggagtacg cgacggccga
     1801 attttcacgc cgcacgccag ggtagctcag cggtagagca ggggactcat aagcccttgg
     1861 tcgcgagttc gactctcgcc cctggcagtc tctaaattac cgattatcga ttgccgattg
     1921 gatccggcgt tggacgttgg atgttcggtg ttcgaccttt tctgtttcgg tttggatgaa
     1981 tgacagaaac gcagtcgaga aactcttcgc ccgcggcaat tcctactggc tgacgcgctt
     2041 tgttattctg cggttgctcg ggttcgttta cgcgatcgcg tttttcatcg cggccaaaca
     2101 actcgtgccg ctgatcggcg accatgggct gacaccggca acgcattttc tcagcgcaat
     2161 tgaagcgcag ctcggctcgc gtagcgccgg cacgttgcat ctgccgacac tcttttggtt
     2221 tggactttcg gaccgcggat tgtcgatgtt tgcgtggatt ggcttcgctc tgtcgctcgt
     2281 ggttcttggc ggctacgcca acgcgatcct gctcaccctc ctctggagca tgtacatgtc
     2341 gatcgtccac gtcggccaaa tctggtacgg ctacggttgg gaaatccaac tgctcgagac
     2401 tggcttctta tcgatcttcc tgtgtccgtt aattgatggc cggccatttc cgaaatgccg
     2461 gcccccgatt ttgatcatct ggctttttcg ctggctcggc tttcgaatca tggtgggagc
     2521 gggtctcatt aaattgcgcg gcgacgcgtg ttggcgcgat ctcacctgcc tctattatca
     2581 ctacgaaacg caaccgatcc cgaatccgat cagccgttat ctccatttcg cgccgcactg
     2641 gtttcacaag ttgggcacgg cttggaacca ttgcatcgag ctgatcgtgc cgtggttctc
     2701 gttttacccg cggctcgcgc gacacatcgc cggagtgctg ctaatcagtt tccaaatcat
     2761 cctgatcatc agtggaaacc tgtcgttcct gaattatgtc acgatcattc cgtttctcgc
     2821 ctgtttcgac gacacattgc tcagccgtat tctgccgaag gtactcgtta ggcaggccaa
     2881 gcgtcgcgcg gcagaatcgc agacatcgca catcaacaac aggatcgccg tcgcattgtc
     2941 gatcttagtc gcatatctca gtgtcgcacc cgtaatcaac atggcttcgg gacaacaatt
     3001 gatgaactac tccttcaatc cgctcgatct tgtgaacacg tacggcgcct ttggcacagt
     3061 cggtcgcgag cgggatgaaa ttattttcga aggaaccgac gacgccgtca ttaccggcga
     3121 tacaaaatgg aaggaatacg aatttctcgc caaaccgggg gatccgaatc ggcggccgcc
     3181 attcgtcgcg ccatatcaac cgcgaatcga ctggcaaatc tggttcgcag ccatgagctc
     3241 gcccgcggag tacccgtgga cgctgcattt cgtctggaag ctgttgcaca acgaccgcgg
     3301 cacgttatcg ctcatcgcaa acaatccgtt tccagatgcg ccgccgcgtt acattcgcgc
     3361 ccgattgttc cgttatcact tcgcaccgct cggcgaaaaa gcgtggtggc agcgggaacc
     3421 gatcggcgaa tggctgccag ctctctcccc tgatgatgcc gagttccggc ggctactcgc
     3481 cgcgatggat tggctcgact gagagatcga caatggaagt agccatcctc ggcgacagtg
     3541 tttgcaccgg cgtctacatc tcatcgccgt ggagcacatt ttggcgcgcg cgcagggctc
     3601 gtgccggcaa ttggtttgtc gacacaactc cgccggcgcg cggcattcaa agcgtttgca
     3661 aaagactcga ggagctcact ccagtcgttg cgaccgaatg cgccggcatt ggtgcgttag
     3721 tcgacggtga ggtcgagcgc cagaattttt ttcgacgaat tcttcgcacc cgcgatttct
     3781 cggggcaaat cagacagctg ctaagagcac cgcgtttgcc cgatctgatt ctcatttcaa
     3841 tcgggcacaa taatatcgac tggacgtggc gacggaccgc gcgcgagttg gaacgcccgg
     3901 atgtaattct acaggaactg agcgttcggt tccgcgaaga ttttgcacgg cacctgcagc
     3961 ggttgctgaa cgtcgcgtct gcgcagtctc atcgcgtggc aatcgtcgtc tacggattgg
     4021 tcaattttga gtcgtacttc aaggcacggg cagtcgctga gcagttgcat gctcaggacc
     4081 gcacgcgcta tccgcatctg gaaaccactt acaaatattt cgtctctttc cgcccgcttt
     4141 accgctcgca tctcgttcgt ctcgcacgga tgctgaacga gcagttgcgc acaatagtta
     4201 ctgatttgaa ttgcgacctc gctgagcatc gcaacgtgca gctccgctat tccgatgcac
     4261 tggcaactgc agaccttagc cgcatcgagt tgctccacgc tgtcgatggc tggcatccat
     4321 ccgctgcagg gcacaacgtc cttgcctccg ctgccttcga tgatcttgct cctaccctgg
     4381 aatttctagg ggttaactcg ccatcaacgg cggcacggca ctgaacgcaa gaccgacatc
     4441 gatacctttc tgtcttccgc cgagcgtcgc gcgaacttga cggctttaag ccgtcaagtt
     4501 gttcggtgcg agtaaatcgt gttgtgccag gtgcacgaca gtctcccgca gcatgcggta
     4561 accgaagtgt ccctggcgca cacgtaacaa ttgcgagccg cgccatcggt ggtggatctc
     4621 tgcgaggtcc tccggccgcg cgatcgtgtc gaactcgccg gaaacaaaca gcacgcggcc
     4681 cggatcgcac gctggctgaa tgtgagttgg cgagctgaga tgaaaatgcc gagcgacgag
     4741 cgccggctcg atgctcatgc ggcgtaactc ctttcgcatc gacgccgtgc ccggactttg
     4801 ccagatcgcg tgctccacgt tgacgatcgg ggccatcaat gcgacaaacc gcaggtcgcg
     4861 ctcgaccgcg gcgagcagcg caccgatcca gccgccgtag ctcgttccga gaataccgac
     4921 ctctgagcag cccatctttc gcagggcgcg gagcagttgc cgcacctcca ttacaccctg
     4981 ccgcagtcct tcggcattgc ggatcaaatc agccgtgatc gcgagttccc cgttccagta
     5041 accgcgcggc acgcgcgagt agtgataggg caaatggaca aagcaggcat tccacccgcg
     5101 ctcgttgaaa ctgcgagcta gccgccggta tccgaaatgc gtggccgaga gcagcgcgtg
     5161 cagcatcaaa accgtgggcg tcgcattcca gccgcgcttg gatggaaaca gatccacgcg
     5221 tgccatgtcg tttgctggga agcgcgtttg gatcgggctg cgccacgtca ggatccgtgg
     5281 agtcgtgcca ttatcgagcg ccgcggcaac atcgacatca tcttgcgcgt aatagtactc
     5341 gtgcacactc aacttttcgc aatctgccac gtagcgctcc atctcttcac gactgcgcgt
     5401 gtcgagccga tgtcgcgatt gcagaaggtt catcgacgcg cacatggcaa agtcgacgct
     5461 ggtcgcagca aagcgtcgcc atggcgcagc tcgaattata ggggaaccac tccggttgcc
     5521 gggtttgtgc gcggcaagcg atgcgcttgc gctagaaccg cgcatccgtt cctgcggcga
     5581 atgcggcaga tcgtccggcg tgagatgaaa ttcttcgcgt aattcggtgt agagcttttg
     5641 aaaggcggct gtcacttccc gctcgattcg ggcgcgcccg acggatttat ccaattgcgg
     5701 gaacgatggg acacattcac cgaatgcgat ccagaccggt gtgcggcgca acggcaacca
     5761 acgtttcaaa ttatacagcc gatcgctgcc gagaatgacg cagggcacga ccggaacacc
     5821 ggccatgtgc gccagggttg atgcacccgc gcggagcggc gccccctcga gcaacgagca
     5881 cgcgccatta cgaatgccgc cttcaggaaa aacaccgacg atgcggccgg ccttgagtcg
     5941 ctcgattgcg gtgcgaatgg tcgcgcgatc agctcgattg cgagcggccg gaaacgcatc
     6001 gacggcgcga aggaacaatc caatgatggg cacgggaaaa aactccgcca tcgccatcca
     6061 gtcgatcttt cgtcgcacga ccgcggagat aatgaacgga tcgaaatggc tgatgtgatt
     6121 cgccgcgagc aaaaatcctc cttcccgatt tgcagtttcc actcgcacga cgtgaacgcg
     6181 cgcgacgcaa ccgaacagca gtttcatgaa cagcgccgcg gcgcgattcg caaccgagtg
     6241 aaatcgagat cgaaccggac tcacattaat tagggagccg ccagctcctg acattttctg
     6301 agatcgagtt tgccggaggc gaggacagga atggcatcga cgcgccagat tttttttggg
     6361 atccacagat ttgggacgcc ggcttcgcgc aatttttcgc gcagctgcgc aagatcgaca
     6421 tctatcgcgg cgaggagaac cagggcttcg ccctttgctt catcttgcac gcccatgaca
     6481 gcaatg
//
LOCUS       DASTDG010000223         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1578706, whole genome shotgun sequence.
ACCESSION   DASTDG010000223 DASTDG010000000
VERSION     DASTDG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578706"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFO30_07355"
     CDS             <1..1099
                     /locus_tag="VFO30_07355"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9419140.1"
                     /translation="ENIAVGLRQVLSDANIEIVPMADGGEGTAEVIGDSLEGSWLKCK
                     AHDPLGREIEVRYVWIADCKLAIMEMSEAAGMRRVAGEKVDVDLATTFGVGEMLLDGT
                     RRGAREIIIGLGGSATNDGGVGMARALGFRFFNHEQEHELTGAVSELTKLARIARPSP
                     GALRPPLPQGENRGEGKSSRVAQPWFPRIIAAVDVRNPLLGPNGATRVFGPQKGATPD
                     KIDILERALTRLADVVAKEFAVDYRNEPGAGAAGGLGFALMSFCGAKIRPGFDVVAEA
                     VGLAAKLRDVDVVITGEGSLDRQTLEGKTPAGVARLARELGKRVFAIVGRASEDRQVC
                     GIFDGVYELGGAIEQARGLLRKKAAELARQL"
     gene            1207..>2204
                     /locus_tag="VFO30_07360"
     CDS             1207..>2204
                     /locus_tag="VFO30_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419141.1"
                     /translation="MTEPPASGLTPFIFADPAGKRWPRLRLIIVIGGMLTFLGAVLFV
                     QTLFVAPKMRVPFSLRQLKGQLKALQKENPAGQVPPSSLLWQKFGAARQAARKLTGAT
                     PAPVAPTRPRKKAPANEVRLAFYTNGDPYSYASLEQHAAFITHLCPEWMTVTNGLGDL
                     QIDADSRLPKFAAAHGIALMPLLTNLVGEAWQPEAIENLAHGPPDRQERFIRSVLAVL
                     RNAKAAGVVIDWEQIDPAYKKEISAFIDKFADALHEDDKQLWLCVQPGQELDYIDFDQ
                     LSDNVDRFVALLFDETSDIDPPGPLGSRSWFEGWLHVLLEDSDTKQWIIALGSYGY"
BASE COUNT          464 a          599 c          702 g          439 t
ORIGIN      
        1 ggaaaatatc gcggttggcc tgcgccaagt tttatccgac gccaacatcg agatcgtacc
       61 gatggcggac ggcggagaag gaacggcgga agtcattggc gattcgctcg agggttcatg
      121 gttaaaatgc aaggcacacg atccgctcgg tcgcgaaatc gaggtgcgtt atgtttggat
      181 cgctgactgc aaactcgcga tcatggaaat gagcgaagcc gccggaatgc gtcgggtcgc
      241 cggcgaaaaa gtagatgtcg atcttgcaac cacgttcggt gtgggggaaa tgctgctcga
      301 tggcacgcgg cgtggcgcgc gcgaaatcat tatcggctta ggcggcagcg cgaccaacga
      361 tggcggagtc ggcatggcgc gcgcgcttgg gttccgcttc ttcaatcacg agcaggagca
      421 cgaattaaca ggagcggtaa gcgaattgac gaagctcgca cgcatcgctc gaccctcacc
      481 tggcgctttg cgtcctccac tccctcaggg agagaataga ggtgagggca aatcatccag
      541 agtagctcag ccctggttcc cgagaattat cgcagcggtt gatgtgcgga atccattgct
      601 cggaccaaac ggcgcgacac gagtttttgg gccgcagaaa ggcgcgacgc cggacaagat
      661 cgacatattg gaacgggcac tgaccaggct cgctgatgtt gtcgcaaaag agtttgcggt
      721 tgattatcgc aacgagcctg gcgcaggcgc ggccggcggc ctgggatttg ctctgatgag
      781 tttttgcggc gcaaagattc ggccgggctt cgatgtcgtg gccgaagcgg tgggcctcgc
      841 agcgaaacta cgagatgtcg atgttgtgat tacgggggag ggtagtctgg atcgacaaac
      901 gctcgaaggg aaaacgccgg ctggcgtggc gcggctagcg cgcgaactcg ggaaacgcgt
      961 gttcgcgatc gtcggtcgcg cgagcgaaga tcgacaagtg tgcggcatct ttgacggggt
     1021 ctacgaatta ggcggcgcga ttgagcaagc acgcggattg ttgcggaaga aggcggcaga
     1081 gctagcgcgt cagttgtagg cgagcgctcc gtttcgcggt cttttcgtcg cagcggatgg
     1141 gacccttaca attttcgatc tgaaccagct ttcattgtcg gatcgtccga gccaaaatag
     1201 cgggatatga ctgagccccc tgcgtctggt ctgacgccgt ttatcttcgc agacccggca
     1261 ggcaaacgct ggccgcgttt gcgcctgatt atcgtcatcg gaggcatgct tacgttcctt
     1321 ggggcggtgc tgtttgtgca gacccttttt gtcgcgccga agatgcgcgt gccattttcg
     1381 ctgcgtcaat taaagggcca actcaaagcg ctgcagaaag aaaacccggc cggtcaagtc
     1441 cctccgagtt cgctgctctg gcagaagttc ggcgcggccc gccaggcagc gagaaaactg
     1501 acgggggcaa caccggcacc ggttgcgcca acccggccgc ggaagaaagc gccggcaaac
     1561 gaagtgcggc tggcgtttta caccaacggc gacccttaca gttatgcctc actcgagcag
     1621 cacgctgcgt tcatcaccca tctgtgtccg gaatggatga cggtcacaaa cgggctcggt
     1681 gatctacaga tcgacgccga ttcgcggctg ccgaagttcg cggccgctca cggcatcgcg
     1741 ctgatgccgt tgctgacgaa cttggtgggc gaagcatggc agcccgaagc gattgaaaac
     1801 ctcgcgcacg gcccgccaga tcgacaagag cgcttcattc gcagcgtgct ggcggttttg
     1861 cgcaatgcca aagctgccgg tgtggtcatc gattgggagc aaatcgaccc cgcatataag
     1921 aaagagatca gcgcgttcat cgacaaattc gctgacgcgc tgcatgagga tgacaagcag
     1981 ctttggctgt gcgttcaacc gggccaggag ctcgactaca tcgattttga tcagctctcc
     2041 gacaacgtcg atcgcttcgt cgcgctgtta ttcgacgaga cttcggacat tgacccgccc
     2101 gggccgctcg gctcccgctc gtggtttgaa ggctggctgc atgtattgct ggaagattcc
     2161 gacaccaaac aatggatcat cgcgctcggc agttacggct acga
//
LOCUS       DASTDG010000224         8947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_371254, whole genome shotgun sequence.
ACCESSION   DASTDG010000224 DASTDG010000000
VERSION     DASTDG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371254"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1105
                     /locus_tag="VFO30_07365"
     CDS             386..1105
                     /locus_tag="VFO30_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET9419142.1"
                     /translation="MAAENSAIATNEETTLSVQRSIYDPGYVNAMSHFYRGEMGRIMV
                     WRQRLDVTTNWAITSSTAIITIAFANRDVPHIIFFFNLAIVWALLWIEARRYRFYDAF
                     RARIRMLEAHFLVPMIMENRDMLQGEWKKLVCEDLILPCFKISKLEAIGRRLKRNYVF
                     IFILILVAWITKIFIHAPMAMDGPTAFYHALRVGHIPSLLVASVMLGTFLCVIGITIY
                     VGKKTSGEISEFGTHRSLWRI"
     gene            1147..1938
                     /gene="mazG"
                     /locus_tag="VFO30_07370"
     CDS             1147..1938
                     /gene="mazG"
                     /locus_tag="VFO30_07370"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9419143.1"
                     /translation="MDQSAAGESFRRLCEIVAQLRAPGGCPWDGEQTHESLLPALIEE
                     AYETAGAIRANDMANFREELGDLLLLIVMHAEIAREAGKFKIDHVLADVTEKLIRRHP
                     HVFGKSDARDSGAVVKQWEAIKRGEKTTDSHYLASLPAGLPALMRAQKAQAKAARVNF
                     DWTELRDVIAKIEEELGETKSAVERQDRQSIQDEIGDLLFAVVNLARKCGFDAETALQ
                     SATDKFVARFNRLEDELRSRGKRLGDVDLGEMDAIWRQIKQTPNA"
     gene            2030..2470
                     /gene="secG"
                     /locus_tag="VFO30_07375"
     CDS             2030..2470
                     /gene="secG"
                     /locus_tag="VFO30_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9419144.1"
                     /translation="MSISISVLLAIWVLVALLMVLVILMQRPKSEGLGAAFGGGVTEN
                     LFGAQTTNVLVKFTAWLAGIFFVLTFALSILYAQKAAGNSALRRELMKQQSAGATSPS
                     PATAKPSPAGSVAASPAPDSGAGVLPAGSVTPAASPAASAAKTP"
     gene            2659..4113
                     /locus_tag="VFO30_07380"
     CDS             2659..4113
                     /locus_tag="VFO30_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9419145.1"
                     /translation="MSIPHLTLWPAAIVVAAIAITTQPVRAQATAESERIEKLERAVE
                     QLQKRNTELEQEVSSLKKHTSFAPLLGPEGKTKVVSEGKTYVEKAVEEEKKVYVTPGG
                     PEYKLTLGGFIQANFEDGDVSAFEGRFGLTALKDRFRLRRARISLTGEFAEQFDFKIE
                     GDFEQSDAAITALKSVDLSTGKTTTVSNSTRTEFSGTDIFINWHGIPEANIKLGQWKA
                     PFGLEQITPDTKLFMIERSLPTGALTPERQIGVQIWGKPLTNVWPEEKDLVTYYAGIF
                     NGNGRNVNTNDNNNFMYVGRLELMPFKGKVFGQEASLKLGGDVLNSRDDAGTNISQTL
                     NLKVNTDGSLSSFTLPGADERTAWSVDAWLNVGPFDLIGEYFSEEVNGRTVNGVAPGF
                     ADFEPSGWYVQGSYFIVPKKLQAAVRFEDLNPGQLGSDGIHSITGGLNYYINGDAIKL
                     MVNYIHTWSDFREAHPEFGDDQFDEVIGRVQVMF"
     gene            4192..5076
                     /locus_tag="VFO30_07385"
     CDS             4192..5076
                     /locus_tag="VFO30_07385"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET9419146.1"
                     /translation="MPGFSSNEGHSDYSLGAGSISTAPRFSFDSVAEEPESVRQVRAA
                     WISDVHLGTRGSNPGALLEFLRDYEFETLYIVGDLIDIWSLRRGIYWPQLHNDVIQKI
                     LRKARKGARVVYIPGNHDELVRDFFGSYGKIDIQRSALHVTANGERVLIIHGHELDTV
                     VQNVKWLAFAGDVGYQFLLALNPLINFIRRRFGLGYWSLSAYAKKRVKDAVSFVGKFE
                     AAVAHYAERHRVDAVLCGHIHSAAIRKFGAVTYYNCGDWVETCSALVEQMDGKIEMVN
                     YLSAVDRPAAGSVCVGAN"
     gene            complement(5115..5558)
                     /locus_tag="VFO30_07390"
     CDS             complement(5115..5558)
                     /locus_tag="VFO30_07390"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9419147.1"
                     /translation="MKSKVLFICVHNSARSQMAEAWLNQICGEFFEARSAGLEPGTLN
                     PLAVEVMREAGIDISKKETRAVFDVFKAGQVFAYVITVCDESSAEKCPTFPAPAKRLH
                     WSFPDPSQLTGTPAEKLARVREIRDQIRAKIDNWCAEVCAAQPVA"
     gene            5708..5935
                     /locus_tag="VFO30_07395"
     CDS             5708..5935
                     /locus_tag="VFO30_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419148.1"
                     /translation="MKVLKVKRVIRKASAHERSTMGAAPGEFMRHYFTHESWWHFAIE
                     ALVFAALAAISAWPIFAAIDAMSKFLPGVAG"
     gene            6149..6619
                     /locus_tag="VFO30_07400"
     CDS             6149..6619
                     /locus_tag="VFO30_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET9419149.1"
                     /translation="MKHRNSREIERKFLIKRLPVEILKSRHFPLLQGYLANEPRGRHV
                     RLRKKGKAASLTFKVSRGNSAREEREIRLSAKQFATLWPATAGRRLRKTRYEIPWKDL
                     KIEIDIYRGHNQGLIVAEVEFPDQTSCRKFKPPNWFGREVTGEKRYSNVTLANE"
     gene            6632..7522
                     /locus_tag="VFO30_07405"
     CDS             6632..7522
                     /locus_tag="VFO30_07405"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9419150.1"
                     /translation="MSYRLKSRESPASGLRRICCEEIKAALAETKSRGRPPGAAVHEF
                     RKHVKKLRAALRLTADEIGKVRYRREDRALRDIAKLASDLRDAHVRLQVIVQLHKKFR
                     GAGFARVFRRIEELLSLELSSFTAACAGWEAKVANRFKAIEKHISSWRLNRLSWKQVC
                     SAVAASYRRGRNQLADTLKKPVPKNFHKWRREVKDLWYQLRLLSPLNRTVLEEIARDA
                     ETLGDLLGRDHDFAFLLSRLDLERNDQVLRRERTKLERLIKKRSKRLQRDAAELGRHF
                     YAEPPKAFAKRISIFVKDRR"
     gene            7530..8249
                     /locus_tag="VFO30_07410"
     CDS             7530..8249
                     /locus_tag="VFO30_07410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9419151.1"
                     /translation="MKYRALACDYDGTLAHDGLLNAATAAALDRFRASGRELIMVTGR
                     ELPDLKTVCHVLDRFEYVVAENGALLYYPEEDCARLLCAPASSELVAALRAANVRPMS
                     VGQAIIATVEPNELIVIEEIKRLGLELQVIFNKGSVMVLPTGVNKATGLGIALKEMAI
                     TANEVVGIGDAENDHAFLGMCAIGVAVDNALSTLKERADFVTSGAQGAGVIELIDMML
                     VTDLSEIVPRPREPQPATTSS"
     gene            complement(8261..8749)
                     /gene="sixA"
                     /locus_tag="VFO30_07415"
     CDS             complement(8261..8749)
                     /gene="sixA"
                     /locus_tag="VFO30_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HET9419152.1"
                     /translation="MAKSKVDLFFLRHGLADWPNWKGSDDDRPLTDEGREETHRVASF
                     LAALGVRPRKILTSPLPRAAQTADIAAKHLRVPIETAKSLAKGFDVDALKRTLTDDKI
                     DSVMLVGHEPDFSRVIAKLTGGDVKLKKTGVARLDLDRRTMTARLRWLLTPAICQKVG
                     AK"
     gene            complement(8761..>8947)
                     /locus_tag="VFO30_07420"
     CDS             complement(8761..>8947)
                     /locus_tag="VFO30_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419153.1"
                     /translation="VVPCLKQALIADYVVLGGGNAKLLQKLPSGVALGHNRNAFTGGW
                     RLCQENPRTSEPTWNII"
BASE COUNT         1956 a         2476 c         2623 g         1892 t
ORIGIN      
        1 tttttcggga tgatgctgcc gcacgacctc gtcgatgatc cgcttggtca ctgcgggaaa
       61 gacgatcacc atcagcgttc cggtgatggc gcaggcgagt gtgccagccg ccatccacgg
      121 gtaacgcttc aggtaagcca aaactcgcca gactgttttc atgcgatgcg aatcacaatt
      181 ccacagcttg gcagcacgtg ccagatgtag ccacccgccg cggcgggcgg cacggaacgt
      241 cgcaaagcgc catcgccggt ttccctgcgc gcaaatgcgg cgcgacattt tcttcatcga
      301 gaacaccgac aagtgcgatg gccatggcgc cgcgagattt gttggattat cgcgttaaag
      361 cagttatcca gttaacgaga gagccgtggc cgccgaaaac agcgcaattg ctaccaatga
      421 ggaaacgacc ctgtcggtgc agcgcagcat ttacgatccc ggctacgtca atgcgatgtc
      481 gcatttctat cgcggcgaga tgggacgcat catggtttgg cgacagcggc tcgacgttac
      541 gaccaactgg gcgatcacca gcagcacagc catcatcacc atcgccttcg cgaatcgcga
      601 cgtgccacac atcattttct tctttaatct cgccattgtc tgggcgctgc tctggatcga
      661 ggcgcggcgg tatcgttttt acgatgcatt ccgcgcgcgc atccgcatgc ttgaagcgca
      721 cttccttgtg ccaatgatca tggaaaaccg cgacatgctg cagggcgagt ggaagaagct
      781 cgtttgcgag gatttgatcc taccctgctt caaaatcagc aagctcgagg cgatcggtcg
      841 ccgattgaag cgcaattacg ttttcatctt cattttgatc ctggtggcgt ggatcacgaa
      901 aattttcatc cacgcgccga tggcgatgga tgggccaacg gcgttttatc acgcgctccg
      961 cgtcggccac atcccatcgt tgctcgtggc gtccgtcatg ctcggaacct tcctctgcgt
     1021 catcggtatc accatctacg tcggtaaaaa aacttccggc gaaatttccg agtttggcac
     1081 gcatcgttcg ctctggcgca tctagctgat gtttgatgtt cgatgtttgg tgtttgattg
     1141 gttctgatgg accaatccgc ggctggtgaa tcgttccggc gtctttgcga gatcgtcgca
     1201 caactgcgcg cgccgggcgg ttgcccgtgg gacggcgagc aaacccatga atcgctcctg
     1261 ccggcgctga tcgaagaagc gtacgaaacc gccggcgcga tacgggcgaa tgacatggcg
     1321 aattttcgcg aagaacttgg cgatctgttg ttgcttatcg tcatgcacgc ggaaatcgcg
     1381 cgtgaagccg gcaaattcaa gatcgaccat gtgcttgctg atgtgacgga gaaactcatc
     1441 cggcgtcatc cgcatgtgtt tggcaagagc gacgcgcgtg attccggcgc ggtcgtgaag
     1501 caatgggaag cgatcaagcg gggcgagaag acgaccgaca gccattacct cgccagtttg
     1561 ccggcaggct tgcccgccct tatgcgcgcg caaaaggcgc aagcgaaagc cgcgcgcgtc
     1621 aacttcgact ggaccgaact gcgcgatgtg atcgccaaaa tcgaggaaga acttggcgaa
     1681 accaaatcag ccgttgagcg gcaagatcga caatcaatcc aggatgagat tggcgatttg
     1741 ctcttcgccg tcgtgaatct ggcccgtaaa tgcgggttcg atgcggagac cgccctgcaa
     1801 tccgccaccg ataagtttgt cgcccgattt aatcgcctgg aagacgagct gcggtcgcgc
     1861 ggcaaaagac tgggcgatgt cgatcttggg gagatggacg cgatttggag acagattaag
     1921 caaacgccca acgcctagca ccaatacttg aatcgctctg aattcgacgt tcgacgttga
     1981 gcgttgggcg tttgcttgtg cctttcgcgt cgccatattg cccgctccga tgtcgatctc
     2041 aatcagcgtg ttgctcgcga tctgggtgct ggtggcattg ctcatggtgc tggtcatcct
     2101 gatgcaacgg cccaagagcg aagggctcgg cgcggcgttt ggcggtggcg taacggaaaa
     2161 cctgtttggc gcgcaaacaa ccaatgtgct cgtaaaattc accgcctggc tggccggcat
     2221 tttcttcgtc ctcaccttcg cattatcgat cttgtacgcg caaaaggcgg cgggtaacag
     2281 cgcgctgcgg cgcgagctga tgaaacagca aagtgccggc gcgacttcgc catctccggc
     2341 aacggcaaag ccttcgccgg ccggatcggt tgctgcttct ccggcgcccg attccggcgc
     2401 gggcgttttg ccggcgggga gtgtaacacc cgcggcttcg cccgctgcga gcgcggcgaa
     2461 gacgccgtag tctatagcgg ccgccgtctc ggcggggact ctgacagagg ggagaatgcc
     2521 gctgcggaca gcggccacta atgtcacgcg attgttacct cagcgacatt tggctgtaac
     2581 aatcgttttc tagcgtccgt tacgtctgca gcagatcaga cgcacagata aaaacctcac
     2641 tacacaaagg agaaacgaat gtccattcct cacctcacct tatggccggc tgcaatcgtc
     2701 gtggccgcaa ttgccattac aactcaaccg gttcgggcgc aggccaccgc cgaatccgag
     2761 cgaatcgaga aactcgagcg cgccgtcgag cagttacaga aacgcaacac cgagctcgag
     2821 caggaagtca gcagtttgaa aaagcacacc tcgtttgcgc ctctgctggg gccggaaggg
     2881 aagacgaagg tagtctccga gggtaagacc tatgtagaaa aagcggtcga ggaagagaag
     2941 aaggtttacg tgactcccgg cggaccggag tacaaactga cgctcggcgg atttattcag
     3001 gcaaacttcg aggacggcga cgtctcggcg ttcgaaggcc ggttcggcct gaccgcgctc
     3061 aaagatcgat ttcgcctgcg ccgcgcgcgc atcagtttga ccggcgaatt tgcggaacag
     3121 tttgatttca agatcgaggg cgactttgag cagagcgacg cggcgatcac cgcgctgaaa
     3181 tcggtcgatc tgtcgactgg caagacgaca actgtctcga actcgacgcg cacggagttc
     3241 tccgggacgg atattttcat caactggcac ggaattccgg aagcgaacat caagctcggg
     3301 caatggaaag cgccatttgg cctggagcag atcacgcccg acacgaagct gtttatgatc
     3361 gagcgcagtt taccgacagg cgcgctcacg cccgagcggc agatcggggt gcaaatctgg
     3421 ggcaaaccgc tcacgaacgt ctggccagaa gaaaaggacc tggtgactta ttacgcggga
     3481 atttttaacg gcaacggccg caatgtaaat acgaacgaca acaacaactt catgtatgtc
     3541 ggccggctcg agttgatgcc gttcaaagga aaagtgttcg gccaggaggc cagcctcaaa
     3601 ctaggcggcg acgttctgaa cagtcgcgac gacgcaggca caaacatctc gcagacgctg
     3661 aatctgaagg tcaataccga cggctcgtta tcgtccttca cgcttcccgg cgcggacgaa
     3721 aggacggcgt ggagcgtcga cgcgtggctc aacgttgggc ccttcgatct gattggtgag
     3781 tatttctccg aagaggtgaa cggtcgcacg gtcaacggtg ttgcacccgg atttgccgat
     3841 ttcgagccca gtggttggta cgttcagggg agctatttca ttgtgccaaa gaaactccaa
     3901 gcggcagtga gattcgaaga ccttaatccc ggtcagttgg gtagcgacgg catccactcg
     3961 attaccggcg gactcaacta ctacatcaac ggcgacgcga tcaagttgat ggtgaattac
     4021 attcacacct ggtcggactt ccgcgaagcg catccggagt tcggcgacga tcagttcgac
     4081 gaagtgatcg ggcgtgttca ggtgatgttc taactgcgcc gatcatgtcc gctgcgccgg
     4141 cgatcaattt cgatcgtggt taggtcagga aaagcccggc gaaccgctgt tttgccgggc
     4201 ttttcgtcaa atgaagggca ttccgattat tcgttaggcg cgggatcgat ttccaccgca
     4261 ccgcgattct cattcgactc agtcgcggag gaaccggagt cggttcgcca agtgcgcgcg
     4321 gcatggattt ccgatgtcca tttgggcacg cgcggcagca atcccggcgc gctgctggag
     4381 tttctgcgcg attacgaatt cgaaacgctc tacattgtgg gcgacttgat cgacatctgg
     4441 tcgcttcgcc gcggcattta ctggccacaa cttcataacg acgtgatcca gaaaattctg
     4501 cgcaaagcgc gtaaaggcgc acgcgtggtt tacattcctg gaaaccatga tgagctggtc
     4561 cgagattttt tcggcagcta cggaaagatc gacatccagc ggagcgcgct tcacgtgacg
     4621 gcaaacggtg agcgcgttct gattattcac ggccacgagc tcgacacagt cgtgcaaaac
     4681 gtcaagtggc tcgctttcgc gggcgacgtt ggctaccagt ttctactcgc gctcaatccg
     4741 ctcatcaatt ttatcaggcg gcgtttcggc ctgggttatt ggtcgcttag cgcttatgcc
     4801 aagaaacgag tgaaggatgc ggtcagtttc gtcggcaaat ttgaagcggc cgtggcgcat
     4861 tacgccgaac gccaccgtgt cgatgcagtc ctttgcggtc acatccacag cgcggccatc
     4921 cgaaaattcg gcgctgtgac ttactacaac tgcggcgatt gggtcgaaac ctgctcggcc
     4981 ctggtcgagc aaatggacgg caagatcgag atggttaatt atttgtcagc ggtcgaccgg
     5041 ccggcagcgg ggtcagtttg cgtaggtgca aactgaaccc ggcgcaaacg gccgcgattc
     5101 cctcggaaag cggactaagc aacaggctgc gccgcgcaga cttcagcgca ccagttgtcg
     5161 atcttcgcgc gaatctggtc ccggatctcg cggacgcgcg ccagtttttc cgccggcgtg
     5221 ccagtgagtt gcgacggatc gggaaagctc cagtgcaatc gcttggccgg ggcgggaaag
     5281 gtcgggcatt tctccgcgct cgattcatcg caaacggtga tgacgtaggc aaaaacttgg
     5341 ccggccttga agacgtcaaa gacggctcgc gtttcttttt tcgaaatatc gataccagct
     5401 tcgcgcatta cttccacggc aagcggattg agtgtgcccg gctcgagtcc ggcgctgcgc
     5461 gcctcgaaaa actcgccgca gatttgattg agccatgctt cagccatctg gctgcgcgcg
     5521 ctgttgtgca cacagatgaa gagcactttt gacttcacgc gagttaacga ttctacggcg
     5581 cgtcgcctgg acacaaaagt aacgtttttg tgacgacgga cacgggccgc gcgtcaccga
     5641 atcgtcacgc cggttttgac aatttgtaac ctgactttgc gctggcgcgg gttatggaac
     5701 gtccactatg aaggtgctta aagtgaaacg agtcatcagg aaggcgtcgg cgcacgaacg
     5761 atcgacaatg ggtgcagcgc ccggtgaatt catgcgccac tattttacgc acgagagctg
     5821 gtggcacttt gccatcgaag cactcgtttt cgccgcgctg gcggcgatct cggcatggcc
     5881 gatctttgcc gccatcgatg ccatgagcaa gtttttgcct ggagtggcag gctaaggcgg
     5941 ttgctaaaac atccggcgaa gagagcgtga cgcctggcaa acgcggcgtc atcgtcccgc
     6001 gcgcttggat catgccagca ttctctgtgg agtgggcgct attcctctgg cccactaaac
     6061 gccccgacga gcgaaggcgg gagcctattg caccgcggca taaaatccgt ttcactccag
     6121 gcaaatttgg caggatttac cccacttaat gaaacacaga aacagtcgcg aaattgagcg
     6181 gaagttcctg atcaagcgcc tgccggtcga aatattaaaa tcccggcact tccctcttct
     6241 gcaaggctac ctggcgaacg aaccgcgtgg ccggcacgtg cgcttgcgaa aaaaaggcaa
     6301 ggccgcgtcg ctcactttca aggtcagtcg gggaaactcg gcgcgtgagg agcgcgaaat
     6361 caggctgagt gccaagcaat ttgctacgct ttggccggcg acggcggggc gtcgcttgcg
     6421 caagacgcgc tacgaaattc cctggaagga tttgaagatc gaaatcgaca tttaccgtgg
     6481 ccacaatcag ggtttgatcg tcgcggaagt ggagttcccc gaccagacga gctgccgtaa
     6541 atttaaacca ccgaactggt ttggtcgcga agtgaccggc gaaaagcgct acagcaacgt
     6601 caccctcgcc aacgaataat ttgttctgtt tatgagctat cggttgaagt cccgcgagtc
     6661 tccggcgagc gggctgcggc gcatttgttg cgaagagata aaagctgcgc tagccgagac
     6721 caaatcgcgc ggtcgaccgc ctggcgctgc ggtgcacgag tttcgcaaac acgtcaagaa
     6781 attgcgcgcc gccctgcggc tgaccgccga cgagatcgga aaagttcgat atcgacgtga
     6841 agatcgtgcg ttacgagaca tcgcgaagct agcctccgat ttgcgcgacg ctcacgtgcg
     6901 attgcaggtg atcgtccaat tgcacaaaaa gtttcgcggc gcgggctttg ccagggtgtt
     6961 tcgccggatc gaagagctgt tgtcgctgga gctgtcgagt ttcaccgcag cctgcgcggg
     7021 ttgggaagca aaggtggcta accgtttcaa ggcaattgag aagcacattt cctcctggcg
     7081 tcttaaccgg ctaagttgga aacaagtctg cagcgcagtc gccgcgtcct accggcgtgg
     7141 gagaaatcaa ctggccgata cgctcaaaaa gccggtgccg aaaaattttc acaaatggcg
     7201 ccgtgaagta aaggatctct ggtaccaatt gcggctccta tcgccgctca accgcactgt
     7261 gcttgaagaa attgcccgcg acgctgagac cctgggcgat cttcttgggc gagatcatga
     7321 ttttgctttt ctgctttcgc ggctcgactt ggaacggaac gaccaggtgc tgcgcaggga
     7381 acggacgaag ttggaaagat tgattaaaaa acggagcaaa agattgcaac gcgatgcagc
     7441 cgagctgggg cgccatttct acgcggagcc gccgaaggca tttgccaaaa ggatctctat
     7501 tttcgtcaaa gatcgacgct gagtaacgag tgaaatatcg cgcactggcc tgcgattacg
     7561 atgggacgct cgcgcacgat ggcttgttga acgccgcaac cgcggctgcg cttgaccgct
     7621 tccgggcttc cggacgcgaa ttaatcatgg taaccggccg ggaattgccg gacctgaaaa
     7681 cggtttgtca cgtcctcgat cgattcgaat acgtggtcgc cgaaaacggg gcgctgcttt
     7741 attacccgga ggaagattgc gcgcgcctgc tttgcgcgcc tgcttcgtcg gaactggtgg
     7801 cagcgttgcg tgccgcaaac gtgagaccaa tgtcggttgg ccaggcgatc atcgcaacgg
     7861 tcgagccgaa tgaacttatt gtcatcgagg aaatcaaacg gctcggtctt gagttgcagg
     7921 tcatttttaa caaaggctcg gtgatggtgc tgcccaccgg tgtaaacaaa gcaaccggcc
     7981 tcggcatcgc cctgaaagaa atggcaatta cggcaaacga agtggttggg attggcgacg
     8041 cggaaaacga ccacgccttc ctgggaatgt gtgcgatcgg cgtcgcggta gacaatgcat
     8101 tgtcgacgtt gaaagagcgg gccgactttg tcacctcggg cgcacagggc gctggagtaa
     8161 tcgagctgat cgacatgatg ctggtcaccg atctgtcaga aatcgtaccg cgaccccgcg
     8221 aaccgcaacc cgcgaccaca tcttcgtgaa gaaagcgggc ttatttagca ccgacctttt
     8281 gacaaatcgc cggcgttaac agccaccgca accgtgcggt cattgttctc cgatcaaggt
     8341 cgagccgcgc cacgccggtt tttttcaatt tgacgtcgcc accggttaat tttgcgatga
     8401 cccgactgaa atccggctca tggcctacga gcatgacgct gtcgatcttg tcatctgtga
     8461 gcgtcctttt tagcgcgtcg acgtcgaagc ctttggccag gcttttcgca gtttcaatgg
     8521 gcacccgtaa atgtttcgcg gctatgtccg ccgtctgcgc cgctcgcggg agtggacttg
     8581 tcaaaatttt ccgcggcctg acgccgagcg cagctaaaaa tgaagcgacg cgatgcgttt
     8641 cctcccggcc ttcgtcggtc agcggtcggt cgtcgtccga tcctttccag ttcggccagt
     8701 cggccagtcc atgccgcaaa aagaacagat cgacttttga ctttgccacg attcaaaaat
     8761 tcagatgatg ttccatgtcg gttcgctggt acgcggattc tcctggcaca agcgccagcc
     8821 gccggtgaag gcgttgcgat tatggccgag cgcaacgccg ctgggcaatt tctgcaaaag
     8881 ctttgcgttc ccgccgccga gcacgacgta atccgcgatg agcgcctgct tcaaacatgg
     8941 aacgacg
//
LOCUS       DASTDG010000225         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_522427, whole genome shotgun sequence.
ACCESSION   DASTDG010000225 DASTDG010000000
VERSION     DASTDG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522427"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFO30_07425"
     CDS             complement(<1..626)
                     /locus_tag="VFO30_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9419154.1"
                     /translation="MVLLIGNYVPDRWPSMLHFGRMMLDGLRRSGVEAVLIRPPAVFG
                     RIQFFGAAAAKWLGYIDKFILFRWRLWRRLSARPAVVHICDHSNATYAQAVGQSPLLI
                     TCHDLFSVRAALGEETDCPPSFTGRILQRWILRGLKSADVLACVSNYTAEDVKRLVTS
                     CSGRPRLAVVPLGLNRPFQLIASDLARARLAQIGSLDLSRAFILHVGS"
     gene            complement(607..2130)
                     /locus_tag="VFO30_07430"
     CDS             complement(607..2130)
                     /locus_tag="VFO30_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419155.1"
                     /translation="MAMACGLRPDLHSRRGKLIVLRRCSDWAMNIDNRQEKAKHRVRQ
                     LLWLFFWLLLLEGVLRKWIVPQLSDPLLIVRDPIVLLIYYFAIRARIFPRNFWVFLLG
                     VIAFFAVATSLIQLWPYLPPAKTLLVVGYGFRSNFFQLPLIWIIALVFGPEDVKKLGW
                     WTLVFLLPLTMLMVAQFSAPPDAFVNRTAGGGSEIMMSALGKVRTSGTFTFVIGVVAY
                     EAMAAAFLIWAALRRDVYKTRLLIVAGVALVVGVSVSGSRSVVGACALVIASLLVVLF
                     MRADLLNRFGRALIVALILGVIISRTPLVREGMQVLSVRFSEVNEGEQSIARSLLERP
                     IAVFEEGFSVLSKAPLFGYGVGVGTNAGAKFLTGRSVFLLSEGEWSRILLESGPLLGI
                     AYLAWRTALVGRIGWLTLKSVRRGNLLPLLLFSASFLPLVSGQLGQPTILGFAVFVSG
                     LTLAARNTETESAVSVQPQPPARAASAQRVVRGRSVYAERMHAQANRQIEKNGSADR"
     gene            complement(2139..2993)
                     /locus_tag="VFO30_07435"
     CDS             complement(2139..2993)
                     /locus_tag="VFO30_07435"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9419156.1"
                     /translation="MKRFLKRHFNRFLPDGARRALRRRWKAQFHPPTDAEFLLERVDS
                     VLRCKVGNRFSFLAPLACDEQLVRFTSGDGRAEFHATTCAAVQGGVLFDIGAHSGVIS
                     ALFCAAAPHNRAFAFEPSPILVRALREIRDLNEFGERMRIEKIGIGDRQATAEMLVDP
                     ASGFVQTRRFEQTMWEQPQHIEVRLERIADAAARLGVVPDFIKLDVEGFEYEAIKGSL
                     DFLACHKPTIFLELHLNYLEERKLPGRAVVGMLQGCGYSFYSYSGVRLHGPKLYNSPL
                     AGINVVAR"
     gene            complement(2990..>4153)
                     /locus_tag="VFO30_07440"
     CDS             complement(2990..>4153)
                     /locus_tag="VFO30_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9419157.1"
                     /translation="KKSYPVLADHIHGKLRRNYALAADKIDIHPFRETVRLLAGSLGL
                     TNLTGGESAWASIDQVCQSLDCETARRLRRGDYGANVRAVYAYEDCAEQQFVAARALG
                     LRRVYDLPIAYWETAQRLLREEAQRYPEWEPTLGGTQDSDAKLARKTRELALAELVIC
                     PSKFVLESLPPDTGTTRHTILAPFGSPPVEVEPPSGADLTTRRGDRPLRVLFAGALTQ
                     RKGLADLFAAMKLINSPQLELVVMGSLLRPLSWYREQFPQFVYAPPRAHVDVLRLMQT
                     CDLLALPSIVEGRALVQQEAMACGLPVIGTRNAGADDVIVDGETGFLVPIRSPGAIAE
                     KLGWFLANRSRIPELAVAARRRAGELTWRGYGEIVVRAVSALLEIDKSMPASV"
BASE COUNT          953 a         1294 c         1214 g          692 t
ORIGIN      
        1 ttcgagccga cgtgcaaaat gaacgcacgg ctgagatcaa gcgagccgat ctgcgccaat
       61 cgggcgcgcg cgaggtcgct cgcgatcagc tgaaatgggc gattcaagcc gagcggcaca
      121 actgcgagcc gcggccgccc agaacaactc gtgacgagcc gcttaacgtc ctccgcggtg
      181 tagttcgaca cacacgcgag cacatcggct gatttcaagc cccgcaaaat ccagcgttgc
      241 agaatccgac cggtgaacga cggcgggcaa tcagtctctt cgcccaatgc cgcgcgcacg
      301 gaaaacaaat cgtggcaggt gatgaggagc ggagattgcc ccacggcttg cgcataagtc
      361 gcattcgagt gatcgcaaat gtggacgaca gccggtcgcg cggagagtct gcgccacaaa
      421 cgccatcgaa agagaatgaa cttgtcgatg tagcccaacc atttcgcagc cgcggcaccg
      481 aagaattgta tgcgaccaaa gaccgcgggc ggccgaatca ggacagcttc gacgccggat
      541 cggcgcagcc catcgagcat catccggcca aagtgcaaca tgctcggcca ccgatcgggc
      601 acgtagttac cgatcagcag aaccattctt ctcgatctgg cggttcgcct gcgcgtgcat
      661 tcgctcggcg taaacggagc ggccacggac gactcgttgg gccgacgcag cacgcgctgg
      721 cggttgtggc tggaccgaaa ccgccgattc ggtttccgta ttgcgcgcgg ccagcgtgag
      781 cccgctgaca aaaactgcga acccaaggat agtcggttga cctagttgcc cactgaccag
      841 aggaagaaag ctcgcgctaa agagcagcag tgggagaaga ttcccgcgac gcaccgactt
      901 gagtgttaac cacccgattc gcccgaccaa cgcagttcgc caggccagat acgcgatgcc
      961 taacaacggg ccgctctcga gaagaatgcg cgaccactcg ccttcactca gcagaaaaac
     1021 actgcgccca gtcagaaatt ttgcgcctgc gttcgtgccg acgcccacgc cgtagccgaa
     1081 aagtggagct ttgctcaaaa ccgagaatcc ttcctcgaac actgcgatag gccgttcgag
     1141 caggctgcgc gcaatcgact gctcgccctc attgacttca ctgaaacgga cggagagcac
     1201 ctgcataccc tcgcggacaa gtggcgtgcg gctgattatc actccaagta tcagggccac
     1261 gatcagcgcc cgaccgaagc ggttcagcag atcagcacgc atgaagagca cgaccagcaa
     1321 cgaagcgatg accagggcac acgcgcccac taccgaccga ctgcccgaaa ccgaaacgcc
     1381 gaccaccaaa gctaccccag cgacaatgag caatcgcgtc ttatatacat cacggcgcag
     1441 cgccgcccaa atcagaaacg ctgccgccat cgcttcgtac gcgacgacac caatgacgaa
     1501 cgtaaacgta cccgaggtgc gcactttccc aagagccgac atcatgatct cgctcccgcc
     1561 gccggcggtg cgattgacga acgcatcggg cggcgcgctg aattgcgcga ccatgagcat
     1621 ggtgagcggc agcagaaaga ccagcgtcca ccagccaagc tttttcacat cctcaggacc
     1681 gaagacaagc gcaatgatcc agatgagcgg cagttgaaag aaattggatc ggaacccata
     1741 gccgacgacg agcaaggtct ttgcgggcgg cagatacggc catagttgaa tcaaactggt
     1801 cgccacggca aaaaaggcga tcactccgag cagaaaaacc cagaagttgc gcgggaagat
     1861 gcgggcccgg atcgcgaaat agtaaatgag taggacaatg ggatcgcgca cgatgaggag
     1921 cgggtccgac aactgcggca cgatccattt ccgcagaaca ccttcgagca aaagcagcca
     1981 aaagaacagc cagagcaact gccgcacacg gtgcttcgcc ttttcttgcc gattgtcgat
     2041 attcatggcc caatcagagc agcgccgcag gacgatgagc ttcccgcggc ggctgtgcaa
     2101 atccggccgc aagccgcatg ccatcgccac gctgtggctc agcgggcgac aacgttaatt
     2161 ccggccagcg gggaattgta gagcttcggg ccgtgcagac ggactccgct gtagctgtaa
     2221 aaagagtaac cgcagccttg cagcatcccg accacggctc ggcccggtaa ttttcgctcc
     2281 tcgagataat tcaggtgcag ctcgagaaaa attgtcggct tgtggcaggc gagaaaatca
     2341 agcgagccct tgatcgcttc gtactcgaag ccttcgacgt cgagcttaat gaagtcagga
     2401 acgacgccca atcgtgcagc ggcgtctgcg attcgctcga ggcgaacttc aatgtgctgc
     2461 ggttgctccc acatcgtttg ctcgaaccgt cgcgtctgca caaacccact ggcgggatcg
     2521 accagcatct cggcggttgc ttgtctgtct ccgatgccaa tcttttcgat gcgcatccgc
     2581 tcgccgaact cattcaggtc gcgtatttcg cgcaacgcgc ggaccagaat tggcgaaggc
     2641 tcgaacgcaa atgcgcggtt gtgcggagcc gcggcgcaga acagcgcgga gatgactccg
     2701 gagtgcgcgc cgatgtcaaa gagcacgccg ccttgcaccg ccgcgcatgt tgtggcgtgg
     2761 aattcggcgc gtccgtcgcc gctggtgaac cgtaccaatt gctcatcgca ggcgagcgga
     2821 gcgagaaacg aaaagcgatt gccgaccttg caacgaagga cgctatcgac tcgttcgagg
     2881 aggaattccg cgtccgtggg aggatgaaat tgcgctttcc agcgacgcct caacgctcga
     2941 cgtgcaccgt ccggaagaaa gcggttaaaa tgtcgtttga ggaaacgctt cacactgatg
     3001 ccggcataga tttgtcgatc tcgagcaagg cagaaacggc gcgcacaacg atttctccgt
     3061 agccacgcca ggttagctca ccggcgcggc gacgcgccgc gacagccagt tccggaatcc
     3121 gcgatcggtt cgcgaggaac catcccagct tttccgcaat cgcacccggc gatcgaatgg
     3181 gcacgaggaa tcctgtttcg ccatcgacga tcacatcatc cgccccggca ttgcgcgttc
     3241 cgattaccgg aagcccgcac gccatcgcct cctgttgcac gagcgcgcgt ccctcgacaa
     3301 tcgacggcag cgcaagcagg tcgcacgttt gcatgagacg aagcacgtcg acgtgcgcgc
     3361 gcggcggcgc ataaacgaac tgcggaaact gctcgcgata ccaactgagc ggacgaagca
     3421 gcgagcccat tacgaccagc tccagctggg gtgagttgat cagcttcatc gccgcaaaaa
     3481 gatcagccag acctttgcgt tgcgtcaggg cgccggcgaa caagacgcgc aacggccgat
     3541 cgccgcggcg ggttgtcaga tcggccccgc tcggtggttc aacctcgaca ggtggcgaac
     3601 caaatggcgc cagaatcgta tgcctggtcg ttccggtatc gggcggcagc gattccagca
     3661 cgaacttact cggacaaatc accagctcgg cgagcgccag ctcgcgtgtt ttgcgcgcca
     3721 gcttcgcatc cgagtcctgc gtcccgccga gcgtcggctc ccattccggg tagcgctgcg
     3781 cttcttcgcg cagcaagcgt tgcgcagttt cccaatacgc gatcggcagg tcgtagacgc
     3841 ggcgcaagcc gagagcgcgc gcggcaacaa actgttgctc ggcgcaatct tcgtaggcgt
     3901 agaccgcgcg cacgttcgcg ccgtaatcac cgcggcgcag ccgccgcgcc gtctcacaat
     3961 ccaggctctg acaaacctga tcgatactcg cccacgccga ctcgccgccg gtcaaattgg
     4021 tcaggcctaa cgaaccggcg aggagccgga ccgtttcacg gaacggatgg atgtcgattt
     4081 tgtccgcggc gagagcgtag ttgcgccgca gtttgccgtg aatgtggtcg gccagaacag
     4141 ggtacgattt ctt
//
LOCUS       DASTDG010000226        24503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_492425, whole genome shotgun sequence.
ACCESSION   DASTDG010000226 DASTDG010000000
VERSION     DASTDG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 24503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24503
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492425"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="VFO30_07445"
     CDS             <1..1695
                     /locus_tag="VFO30_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET9419158.1"
                     /translation="GSAVDAAIAANAALGLMEPVSNGIGGDLFAIVYSAKENKLYGIN
                     GSGRSPLGLTYEQMKAELDKLHRTMIPPFGMLPISVPGTVDAWAELHNKFGKLKLADD
                     LTPAIRYAEEGFPVSELIAYYWAFGPRLYKDLPGAFFETYTLDGKGRTPAKGDVFKNP
                     ALAKTLRSIGDKGRDAFYKGEIADKIDMFMQQNGGFLRKVDFEKHASTWVDPVSTNYR
                     GYDVFELPPNGQGIATLQMLNILGGFDLRAMGRNSPDTLHTMIEAKKIVWADRAKYYA
                     DPAFSKIPLQILLSKEYAAERRKLIDPMHAAKKVGAGNPDEMQASNARDDRNGTWSSA
                     PCAQRISNPLMSTGSGQNVRWTHRPEAYVPDALDSGDTIYMCTADDQDNMVSLIQSNY
                     RGMGSGIVVPGLGFRFQDRGELFSMEPGHANVYAPGKRPFHTIIPGFVMKDGKPWEAF
                     GVMGGGMQPQGHVQVLTNQIDFGLNVQEAGDASRWQHEGDNEPTGEKMENGGYVNLES
                     GISAETQRELKKRGHDVRFDVGGYGGYQAIKVEWHDGQRVYVGASESRKDGMAAGY"
     gene            complement(1811..2308)
                     /locus_tag="VFO30_07450"
     CDS             complement(1811..2308)
                     /locus_tag="VFO30_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9419159.1"
                     /translation="MPNESRAIDPAFRVGHVHLKVSDLERALNFYCGVLGFELQQRWG
                     TQAAFISAGDYHHHIGLNTWESKGAPPPPPHTTGLFHFAIVYPTRTQLADALRRLIAA
                     NIPLDGASDHGVSEALYLRDPDENGIELYWDKPRNQWPRTPEGKIDMSTRPLDLDDLL
                     QEQEL"
     gene            complement(2324..2893)
                     /locus_tag="VFO30_07455"
     CDS             complement(2324..2893)
                     /locus_tag="VFO30_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419160.1"
                     /translation="MMRRAALFVVIAASAALAQGPESFEIEPPLLIPNRTGAAGSTSD
                     AQPQDVDIAKLEKDLERAKRNADAAPHLCRIGVISQVEVEQRALRVVRLEAELANARL
                     ESAQAQTATRSWVTVNGEAAVDAATKAELAQLTEAAQAADAKRHRVEIELAEANLQRQ
                     LRLLAVGSARKSDVSRAEEKLAQLKGQQN"
     gene            complement(2960..3418)
                     /locus_tag="VFO30_07460"
     CDS             complement(2960..3418)
                     /locus_tag="VFO30_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9419161.1"
                     /translation="MKKPLMLLGLATVVAIGFVAFASGQAEKRSKSATGKTKEVIYAS
                     ADNATYTERFPGVSMAVVWGDPDKGAHGVFTKFAPGYDAHMHTHTNDVWLLVLKGAYL
                     YRDEAGDKHVGPGEFIRIPGGHKHWSGGDQTEGALFYSEGSGKFDKIDVK"
     gene            3548..4870
                     /locus_tag="VFO30_07465"
     CDS             3548..4870
                     /locus_tag="VFO30_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9419162.1"
                     /translation="MNDETISLQQAERVDEQLVRGIGMPALTANIISCTIGAGIFVLP
                     ALVARNLGPAAPLAFVVCAIAMVLFVTCFAIAGSRVSLTGGLYAYVEVAFGRYIGFLA
                     GVLYGLTALGAVAGVVNVLVNSVALVAPFLGSSVMRIIVMMLVYGSLVFINVRGVRGG
                     AGAVTIVTFAKLLPLLLFIIAGIFFIHPANLTWATWPGSKSLGDAVILLIFAFVGIEV
                     ALIPSGEVKNPARTVPRAAYLALVITTIIYILIQVVAQGTLGADLANHPDAPLAEAAA
                     KFLGHVGRTVLLAGATISAFGFVTSDILSSPRMIFAFGRDGALPAFFAHVHPRYRSPD
                     IALITYAVLAFALSITGRFEQLAVLSNVAVLLMYFLCCAACWVLVRQDVRADGRPLNF
                     PGMNIVPALAIVAIIWILAHATAREFTVTAIVLAVASLLYLVRRISLT"
     gene            4933..6018
                     /locus_tag="VFO30_07470"
     CDS             4933..6018
                     /locus_tag="VFO30_07470"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9419163.1"
                     /translation="MKRILILTAGFGEGHNSAARGVRDGLARVAPVQVEVELRDLFPQ
                     SYGALNELVRKTYLAVINQWPHSWGYVYRWLDGIRNFDERFQRFRRLKKHFAQVLDRF
                     QPDVLVSTFPPYPYLLRQIAGADRSCKNVVVITDSITVNAIWYQCDADFFLPSNEQSA
                     AVVRAAGVAPEKIKVFGFPVNPQFADLEQDRQSRRQSARPHILCVIHAGKKRAPELVR
                     KLLGLEIELTVAVGHDRRLHDAIRRVSDKGQIEMIGWTNEMPRLLCRSHILIGKAGGA
                     TVQETIAAGCPMIVSHVVSGQEEGNARLIAETDSGVIALETDEAVAQVSRAFADGAQQ
                     WREWAGNIAKLSRPRAALDIAQFLLSI"
     gene            complement(6004..7161)
                     /gene="nagA"
                     /locus_tag="VFO30_07475"
     CDS             complement(6004..7161)
                     /gene="nagA"
                     /locus_tag="VFO30_07475"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET9419164.1"
                     /translation="MILRNGRCIFPEGIRDGLELVVRDGKIAEIRPQTGFADDEIVDL
                     GGDYLAPGFIDLHLHGAVGRDTMEASADAFRAICDFHASGGTTSLLLTTASAPLDAIV
                     NALDAVRDCRFSNLQIAGVHVEGPFISKTKAGAQRAEFIQHPTPAAMARLLEHVDVIK
                     RFTIAPELPGALDAIAKFAAAGVSVSGGHSDCSDAEARAAFARGLRSTTHTFNCMSSA
                     RRDGIYRRAGLLEFALSEPEISCELIADGHHVSPTLMKMLYRAKTSSGICLVTDATAG
                     AGLEQGSHFSLYGKDCIVDRGVCLLADRSALAGSAARMIDLVRTLVHDLDLPLHEAIA
                     MATQNPARAIGLEAKGALRSGADADLVVLSPALAVRRTFLGGRETFALDRQ"
     gene            7215..8756
                     /locus_tag="VFO30_07480"
     CDS             7215..8756
                     /locus_tag="VFO30_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9419165.1"
                     /translation="MDFDYDVVIIGGAFSGAATALMLKRKQPNARVLIVEKNAEFDRK
                     VGESTTEVSSCYMTRILGLTNYLGHEHLAKQGLRLWFYNSPEQRFDDCVEIGPHYNAR
                     VQTFQVDRAKLDSHLLECAVNADCDLWRPAKVARIDLNAANGQTITVDVDLAQRAVRT
                     RWVIDASGRATLLARKLGHFRPNAEHPINAVWARFTGVKDWDSYDWREKFRDYMDECR
                     TARSWATNHLMGHGWWCWIIPLRGGDVSAGIVYDNRIFKLPDGTNLAGRLHAHLVGHP
                     VGREIFHEARAIEGDVHALSLLPYHSERVCGEGWALVGDAAGFIDPLYSPGLDFCSYT
                     SNYVADFLARSLAGEDVSESVRHYNEQYPIMYRSWFKTLYKDKYYYMGDAELMSAALL
                     LDVASYYFGLVIPAYKDPEKAFLQLPFRGTPGRIAARMISFYNRRLVRLARRRLATGY
                     FGKNNAGWRELYDGFVPDWRATKLLRKGLFRWWKAELTNLRLIFVSPARVRPNESSAP
                     APAEA"
     gene            complement(8796..9893)
                     /locus_tag="VFO30_07485"
     CDS             complement(8796..9893)
                     /locus_tag="VFO30_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9419166.1"
                     /translation="MDISAYRLLFSIFVFITGAVVGSFLNVCIYRLPLDLSINRPRRS
                     FCPACKYPIPWQHNLPLASWMLLRGRCANCGASISLRYFAVELLTAFLFLAIWQSFPI
                     PMAIAYWIFVSLVIVATFVDFEHFIIPDQVTIGGAIMGVLASLAVPQLMETDSRLAAL
                     IRSMLAAALGYLVLLFVLEAGKLAFGKKRIKLDGPTPFTWKRQGDDAEFVVGAEQSLW
                     SDYFAREKDRLILECDDAKIDNRIHNKITLEFYYDRLVVEGETLLLDHVNEITGTAVL
                     IVIPREAMGRGDLKFLAAIGAFLGWRAVLFAVFAGSLLGSLVGLFTLLVGKRVWSAKL
                     PFGPYLAFGAVTWMFYGPAFVRWYLGLLSPS"
     gene            complement(9898..10680)
                     /gene="tatC"
                     /locus_tag="VFO30_07490"
     CDS             complement(9898..10680)
                     /gene="tatC"
                     /locus_tag="VFO30_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9419167.1"
                     /translation="MPLRDIFKFRELPEGETSKPFLEHLEDLRWTIVKMAITLIAAMI
                     VCFGFRSTLVRILQAPLREIGEQAAELRALGITDSIVISFHLAFYAGIVLSFPLLLYF
                     CAEFVLPALTAVEKRFLLPAITVSFALFLLGVLLCYFWLLPKTILFFFRDTQSLGWTP
                     TWTVQQYYSFVTRFTIGFGLAFELPVVVLALVRFGLVTYRFMARTRPYAIVLIFILAT
                     IITPTPDILTLIAMGLPMCLLYESCIWIAWLMERRALQRNVA"
     gene            complement(10693..11304)
                     /locus_tag="VFO30_07495"
     CDS             complement(10693..11304)
                     /locus_tag="VFO30_07495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9419168.1"
                     /translation="MTRSNIVQDTGGWETVSSEHHFTNKHLEVVTDIVRTPMRSQPRP
                     WTVVHRKAAVIIAPMTIDGKLLLIRQERVPIRAAIWEMPAGQIDNGEADDEAIREVAL
                     RELREETGYELAPGGELIALGHYFASPGFTDEYGYLFLARPVKHSARGDGHGEGESIL
                     DCHAFTVGEIRQMIADNEIRDANTLGICARLAAHGFISLAPAE"
     gene            complement(11346..12491)
                     /gene="argH"
                     /locus_tag="VFO30_07500"
     CDS             complement(11346..12491)
                     /gene="argH"
                     /locus_tag="VFO30_07500"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET9419169.1"
                     /translation="MDVALEDVHMNIEAALTHKIGAVGAKLHTARSRNDQIALDLRLY
                     VKTEIANIDNRLRSLEATLLDLAEEHVSIVMPGYTHMQRAQPISFAHYLLAQIEAFER
                     DRERLRACLGHTDVLPLGSGALAGSTIVLDRQFIARELGFARVSQNSLDAVSERDFIC
                     EFLFCLAMIGLHLSRLSEDLILWSTTEFGFVDFSDAFSSGSSLMPQKKNPDVTELTRG
                     KTGRLFGNLMSMLTTLKALPAGYNRDLQEDKHALFDSSDTINLALEVFADALRELKIN
                     REGMEAATADPNLLATDVAEYLVRKGAPFREAHEIIGRIVAQASQSKVPLNEMPLEEL
                     RKFSPLFETDLARVFDPRHSLAQRHAIGAPSPENVAAQIKRWRAELT"
     gene            complement(12461..12709)
                     /locus_tag="VFO30_07505"
     CDS             complement(12461..12709)
                     /locus_tag="VFO30_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419170.1"
                     /translation="MRKGRLAAPLADSAVRYTESVSFDWRLYHEDILGSITHARALAA
                     AGIISADEREKIETGLREIASEIERGECKWMSRSKTCT"
     gene            complement(12711..13079)
                     /gene="folB"
                     /locus_tag="VFO30_07510"
     CDS             complement(12711..13079)
                     /gene="folB"
                     /locus_tag="VFO30_07510"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET9419171.1"
                     /translation="MNGSAGDKIHIEELEIFARVGVSNEERAQPQRLAVNITLWPRAK
                     FRDLSEDITRTIDYSAVCEETRRVAGAESAKLIETLAEQIADHLLRTFPARQVSVELR
                     KFVLADARYAAVFVDRNAED"
     gene            13389..14162
                     /locus_tag="VFO30_07515"
     CDS             13389..14162
                     /locus_tag="VFO30_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419172.1"
                     /translation="MNANAALKGDRQNGFVTTRWSVVLSCGSPEERGSAALAELCRIY
                     WRPIFAFICRRGYSVTDAQDLTQDFFLMVIEGNLLQLADPCRGRFRSLLLKAMQNFLI
                     DSHAWRKAQKRGGKIQFVCWNEWMAEAPSQLSIHTREAESWPAEKIYDVRWAATVVER
                     ALHRLGEECESRGRRRMFSVLSKYLAAERSDVSYVAIGNDLGVSADAIKRLLHRLRDR
                     YRAILREEVLQTVESAAEVDEEIRYLCAVLATEEPPHAQ"
     gene            14159..16942
                     /locus_tag="VFO30_07520"
     CDS             14159..16942
                     /locus_tag="VFO30_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9419173.1"
                     /translation="MNIAGLSDFTGNASDEAPKCECGSAATVSVGLCVSCLLRCGLRA
                     EAATEESFAEALRQIEIRDSDWQLGNYQILEEIGRGGMGVIYRARQRHSGRIVALKRV
                     LSYHSDSHETLTRFRREAEAAASLDHANILPIYEVAMGEDGLPFFSMKFAPGGSLLPA
                     RTTYRARPRQAVQLLAKVARAIEHAHTAGILHRDLKPGNILLDARGEPLVSDFGLAKW
                     LDATSDLTRTMTVFGTPGYIAPEQVRSPAEMLTAAVDVYSLGAILFELLSGRPPFVAE
                     HAIAVIHQAAEKQAPRLRSVAPALSRDLETICARCLERDPRARYQSAGVLAEDLERWL
                     EDRPISVRPVCAPVRLWRWSRRNRRLAVACAACVLLGAVAALRQLQSWHLESAVRKAV
                     LAAHSIAVLPFLNLDTGEMANDMTARLGKALRAELPALGPAKIVELPIPSAKWTGTGN
                     VDEIRIAAGQADTRAVLSGTVRHSGRGVRLSIHLFRETGDDTLGQWLLDVGAIEELPQ
                     ALRRPGGIAALLYRALDARTHAQKGASNTDAAMVDDVARGYFNAGRALLDRRTIPDMD
                     RAINCFEEAVRAAPASVAARSYLALAYLGRNFLTANPAHMERAFAVANQALALDSHNP
                     APHRALCSLYTSTGQFEQALEHGLLALEAGDPSERALGQIAFVWKELGHPEKAIRWFG
                     KAKLSERQPADYDALLGDCWMLLTEDDQAREAYRAAANFHPDLPEGWLGLCHLQLLNG
                     DFAGARMLFRQRAGEYQAFHTVKPLLAQIEFFSRNFAEAERLYREIRDADPRGVGLEQ
                     YGAISSASALARLKFATGDLVSGNQLLKQCLASDQAQVRVSPHNAEVLYRLSADEAIQ
                     GNVAMSLQFLRNSIAAGWLDYRSTRLDPRFDQVAANPEFQKLLSELAAQVAGLRTALT
                     SRSPKGENENSAR"
     gene            16948..17319
                     /locus_tag="VFO30_07525"
     CDS             16948..17319
                     /locus_tag="VFO30_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419174.1"
                     /translation="MPRTWKSKQDRRDAAVAFLRKTITDPDVRSAVLKDRQLAHKLFE
                     REGDINIPDDVEVICVGPSTQERDRLVVFVLPPEGTDTEHLDPFKYWTGTWLPYNMDP
                     MKVVASAERTGARAPEPVAAS"
     gene            complement(17314..18735)
                     /locus_tag="VFO30_07530"
     CDS             complement(17314..18735)
                     /locus_tag="VFO30_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET9419175.1"
                     /translation="MDDYLRPFRESALDRYCRLFRPMRLRADADQYCQALTELGRAMI
                     DDGTRVSDERPEILVDSGYTYFGQFIAHDLTKDVSSVDGAWQKEPEELENLQTPKLDL
                     STLYGGGPEISPELYETDGVRLKVGQRKNGRHSFDIHVAGNGGCVLADERNTENLILR
                     QMTAVFARVHNFAVEQFQGEFADNTALFARARLQTQWQYQWLVCRDYLPTLLDPAVYR
                     EVFVHGRSIIRWNTFSVPIEFAAAAMRFGHAMVRPNYLFSFGQEMRLPQIFSRTSERG
                     PLDEKVEINWGFFFQGAGDGRSVTSRPIDTRLAPSFQLLPDDLVGVAQIACPHFRVAK
                     NPAELPVRTLLRGAGLRLASGQSAARALHERVLSEAELTQNLEGEETAQGQILRDTGL
                     TRDTPLWYYILKESEVRHNGNRVGPLGSRVIAETLYAALRADPNSYLNRVGADDFPPV
                     WRFAEEDVRLYGLSEFFRTADRL"
     gene            complement(18783..19142)
                     /locus_tag="VFO30_07535"
     CDS             complement(18783..19142)
                     /locus_tag="VFO30_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET9419176.1"
                     /translation="MNSTLRNRAVAEWRGSPEHRVEPDRWQATAELLPKMMQRLGLKE
                     RLQESEIMDAWSNIVGEFIAAHSTPVALHDGVLYVRVLQPALHYELEQVSKIDILRKL
                     KRRFGGKVVGDVRFRVG"
     gene            complement(19163..20566)
                     /gene="rpoN"
                     /locus_tag="VFO30_07540"
     CDS             complement(19163..20566)
                     /gene="rpoN"
                     /locus_tag="VFO30_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9419177.1"
                     /translation="MHQTQNLSLQQVLAPQLQQSLLILQAPLLELRNLVQQEMEVNPV
                     LEEMPEDPDAEPRTEADPAADAKFKEEFDKLAKLDEEWRDYMAQSSSYTGRSQEAEDK
                     RQFFFDSIATQETLQQHLMGQLNQTVLNTNDRKTAELIIGNIDDNGFLQTTPEEMALN
                     TGIPQADFENMLTLIQSFYPPGVGARDLRECLLIQLKREGRQNSLEYKVVSEHMQDLG
                     KRRFPEIARRMGISVEQVQKCANNIAQLDPRPGQIFAASPQNYVLPDVTVEKVNGQYQ
                     VILNGEQIPHLRISNTYKDIMAQEGNGNEVKDYIRDKIRSGKFLIKSIHQRQQTISNI
                     AHQIVQRQREFFEHGSSHLKPMTMVQIADAVGVHETTVSRAISGKYIATPQGVFEMKY
                     FFTPGYQTSSGESMSNTSVKEAILDLVKNEDGNAPLSDKEIVEILSKRGIPIARRTVA
                     KYRTELNILPSNMRRKY"
     gene            20717..20941
                     /locus_tag="VFO30_07545"
     CDS             20717..20941
                     /locus_tag="VFO30_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419178.1"
                     /translation="MKRPLFALLLIFFGALSTPAQMPKKDDPQQLLALVKEVQAQQLQ
                     IAANQAKIEAKLAELSETIRVARIYSSRSR"
     gene            20947..21195
                     /locus_tag="VFO30_07550"
     CDS             20947..21195
                     /locus_tag="VFO30_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419179.1"
                     /translation="MKAPLLLLSIFAAVAIFTADAQSPTPMQPVTTDTESIPGAIKVL
                     QEMKTANDEVLKKQEATLQALEELQKAADEIKIFGKRS"
     gene            complement(21226..21987)
                     /locus_tag="VFO30_07555"
     CDS             complement(21226..21987)
                     /locus_tag="VFO30_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9419180.1"
                     /translation="MKDSGPSTREMERINWLEFDEWVPAKIKTVLLPLGTLEAHGVTA
                     NGTDIIAPVAMAREIAPRVNAMIAPAIAYGFTGVLDAYPGSFTIPEAPYREYVRAVMT
                     GLAKNKFKNLILLNGHGGGQTAILAALAQEVGRETGTRILLVNWWSYCSDVTLEVFGE
                     DGGHAGDNETAYVMAIDPALVDEKRFNRDMVTAMPAPNTWSAYPFPSSIMLYNQSQGY
                     PRFDSVKAKSYFAKVNDKIAQLIEETIRKWDMAGL"
     gene            complement(22095..23465)
                     /locus_tag="VFO30_07560"
     CDS             complement(22095..23465)
                     /locus_tag="VFO30_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9419181.1"
                     /translation="MAKILVIDDEPAMVEVIVTLCREKGHQVFPFNSAPKAVEQLDSL
                     APQVVIADIKMETMTGFDVLRHVREHHRQTAVILITAYGTVETAVEAMKMGAYDYVTK
                     PFKIDELQLTVQRALDYQAALRENVYLRKELKNRYRFENIIGTSQKMQQVYNLINKVA
                     DTDSTVLIVGESGTGKELVARGLHFNSNRQHHPFVAINCSALPENLLESELFGHKKGA
                     FTGAVADKRGLFEEANLGTIFLDEVNSMAPPLQTKLLRVLQEREVRRVGDNKSSPVNV
                     RVVGATNEPLLPKLKDGTFREDLYYRLAVIPVEIPALRERLEDVPLLVHHFLQKQASA
                     SGGEPKKIDPAAIDILSHYSYPGNVRELENAIERACALCENELILPSDLPPHIVSEAQ
                     GGKSDGAIAAMPVGQPLDEFIQQQERNYIEATLAQCGGSREKAASMLDISMATLYRKI
                     EPKSKR"
     gene            complement(23524..>24503)
                     /locus_tag="VFO30_07565"
     CDS             complement(23524..>24503)
                     /locus_tag="VFO30_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9419182.1"
                     /translation="QKSFADWIRAINAEKAKLAPEDYVASINNGFTEVKCFGTTTMAN
                     LTAFPELVPQISFPIRTCWFGELIDVRGTADADLAAKFLRSAEHWGLAPHAPFTASAE
                     LYRRCERLASLLTTHIAESHEEMSMFRDASGPLYDFLKELGRDMSDCGGKTPLAWFLT
                     FRDLSTPLGMTKEWLVVHLNELTDSDSNLLEKSPRNFSIVHCPRSHSYFGHSPFQFER
                     LRALGFNICLGTDSLASNDNLSLFAEMRAFQKEFPNVSPEEILQMVTVNPARALQHKG
                     ALARLLAGFRADIIAVPCTKPAAAYEEIVAFDGAVEGTVMDGCVSGHVP"
BASE COUNT         5056 a         7178 c         7170 g         5099 t
ORIGIN      
        1 ggcagcgcag tggacgcggc gatcgcggcg aacgcggcgc tcggattgat ggagccggtc
       61 tcgaacggaa tcggcggcga ccttttcgcc atcgtttaca gcgcgaagga gaacaagctt
      121 tacgggatca acggcagcgg acgttcgccg ctaggcctta cctacgagca aatgaaagcc
      181 gagctcgaca agctacatcg cacgatgatc ccgccgttcg gcatgttgcc gatcagcgtg
      241 cctggcacgg tcgacgcttg ggccgagctg cacaataagt tcggcaaact caaactcgcg
      301 gacgatctca cgccggcgat tcgttacgcc gaggaaggtt ttccggtgag cgaattgatc
      361 gcctactact gggcattcgg tccgcggctc tacaaagatt tacctggcgc cttcttcgaa
      421 acctacacac tcgacggcaa agggcgcaca ccggcgaaag gcgacgtttt caaaaacccg
      481 gcgctggcga agacgcttcg atcgatcggc gacaaaggac gcgatgcttt ctacaaaggc
      541 gagatcgccg acaagatcga catgttcatg cagcagaacg gcggattcct gcgcaaagtc
      601 gatttcgaga agcacgcgag cacgtgggtc gatccggttt cgacaaacta tcgcggctat
      661 gatgtctttg aacttccgcc gaacggacaa ggcattgcta ccctccagat gctgaacatt
      721 ctcggaggct tcgatctgcg cgccatggga cgcaattcgc ctgacacatt gcacaccatg
      781 attgaggcga agaagatcgt ctgggcggat cgcgcgaagt attacgccga tcctgccttc
      841 agcaaaatcc cgttacaaat cttgctctcg aaggaatacg ccgccgaacg gcgcaagctc
      901 attgacccca tgcacgccgc gaaaaaagtg ggcgctggca atccggatga aatgcaagct
      961 tcaaatgcgc gcgacgatcg caacggaaca tggagctctg ctccgtgcgc ccagcggatt
     1021 tccaacccgc tgatgtctac aggcagcgga cagaatgtcc gctggacgca caggccggag
     1081 gcctatgttc cggacgcgct ggatagcggc gacacgattt acatgtgcac ggccgatgat
     1141 caagacaaca tggtctcgct tatccaaagc aactatcgcg gaatgggcag cggcatcgtt
     1201 gtgccgggac tcgggttcag gtttcaggat cgcggcgaac tgttttcgat ggaaccgggt
     1261 cacgcgaacg tttacgcgcc gggtaaacgt ccgtttcaca cgataatccc cgggttcgtc
     1321 atgaaagacg ggaaaccgtg ggaagcgttc ggcgtgatgg gcggcggcat gcaaccgcag
     1381 ggacacgtcc aggtcttgac caaccagatc gacttcggat tgaacgtgca ggaagcgggc
     1441 gacgcgtcac gctggcagca tgaaggcgac aacgaaccga caggcgaaaa gatggagaac
     1501 ggcggctacg tcaatctcga gtccggcatt tccgccgaga cgcagcggga attaaaaaaa
     1561 cgcggacatg atgtgcgatt cgatgtcggc ggctacggcg gttatcaggc gatcaaagtc
     1621 gaatggcacg atggtcaacg cgtgtatgtc ggcgcgagcg aatcgcgcaa ggacggaatg
     1681 gcggcgggtt attgaagcaa acggcgaacg ctcaacgtcg aacacgcaac gttgaattca
     1741 gcaggccaca ttggagcggt gcttgtgcca agcgccggat ctattggtta ggcgtttgac
     1801 acagacgcct ctacaactcc tgttcctgta gcaaatcgtc gagatcgagc ggacgcgtgg
     1861 acatgtctat cttgccctct ggcgtgcgtg gccactggtt gcgcggtttg tcccagtaaa
     1921 gctcaattcc gttttcatcc gggtcgcgca gatacaacgc ttcgctcacg ccatgatcac
     1981 tcgcgccatc cagggggata tttgccgcaa tcaggcgcct gagcgcgtcc gccagctgcg
     2041 tgcgcgttgg ataaacaatc gcgaaatgga agagtccggt cgtgtgcggc ggcggtggcg
     2101 gcgcgccttt gctctcccag gtgtttaaac cgatgtgatg gtgataatcg ccggcggaaa
     2161 tgaaggcggc ttgcgttccc cagcgttgtt ggagctcgaa tccgaggacg ccacaataaa
     2221 aattcagcgc gcgttcaagg tctgacacct tcaggtgcac gtgaccgacg cgaaaagccg
     2281 gatcaatcgc cctcgattcg ttaggcatcg ggtaagcggc gccctagttt tgctgcccct
     2341 tgagctgcgc gagcttctct tcggcgcggc tgacgtcgga ttttcgtgcg ctgccgacgg
     2401 cgaggaggcg aagttgccgc tgcaaattcg cttcagctaa ttcgatctcg acacgatggc
     2461 gcttcgcatc ggccgcttgc gcggcttcag ttagctgcgc cagctctgcc tttgtcgcgg
     2521 cgtcgaccgc cgcctcgccg ttaacagtaa cccagctgcg agttgcggtt tgcgcctgtg
     2581 cgctttcgag gcgggcgttg gccagctcgg cttccaagcg aacgacgcgc agcgcgcgtt
     2641 gctcgacctc cacctgagaa atcacgccaa tgcgacagag atgtggcgct gcgtccgcgt
     2701 tgcgcttcgc gcgctcaaga tctttctcta gcttcgcaat atcgacgtcc tgcggctgcg
     2761 cgtcgctggt cgaaccggct gcgcccgttc gattcggaat tagcagcggc ggttcgatct
     2821 caaagctctc cggcccctgt gctaacgccg cgctcgcggc gatgaccacg aaaagcgccg
     2881 ctctccgcat catggctgaa tatacaacga ggcgaaatcg acctcagtgg acccgagcgg
     2941 cgcgtgttgt ccgctcggtt tatttcacgt cgatcttgtc gaacttgccc gacccttcgg
     3001 aatagaagag cgcgccttcg gtttggtcgc ccccgctcca gtgcttgtgc ccgccgggga
     3061 tgcggatgaa ctcgcccgga ccgacgtgtt tgtcgccggc ctcatcccgg tacagatagg
     3121 caccttttag cacgagcagc cacacgtcat tcgtgtgggt gtgcatgtgc gcgtcatagc
     3181 cgggtgcaaa tttcgtgaac acgccgtggg cgcccttgtc cggatcgccc catacaactg
     3241 ccatcgagac gccagggaaa cgctccgtgt aggtagcatt gtccgccgag gcatagatta
     3301 cctctttggt cttgccagtt gcagatttgc tccttttctc cgcctgaccg gaggcaaatg
     3361 caacgaagcc aatcgcgaca actgtcgcga gtccaagcaa catcaatggt tttttcatag
     3421 tttgtcctcc gtgaattgat tctcagtcta gttgtcgatg ttgcgcgttg tcgatctttc
     3481 ccgcaacgga aaggttggcc gctggggcgc ttgtctctgt tgcggcaatc ggccacgatg
     3541 cacaaacatg aacgacgaga cgatatcgct gcagcaagcg gagcgagttg atgagcaact
     3601 cgtgcgtggc atcggaatgc ccgcgctcac ggcaaacatc atcagctgta cgattggcgc
     3661 cggtattttc gttttgccgg cactggtagc cagaaatctt ggtccggccg cgccgctcgc
     3721 gttcgtcgtc tgtgccattg cgatggtgtt gttcgtcacc tgtttcgcga tcgcgggcag
     3781 ccgcgtctcg ctcacgggcg gactttacgc ctacgtagaa gtggcgtttg gccgctacat
     3841 cggatttctt gccggcgtgc tctacgggtt gacggcgctt ggggcagtcg ccggcgtggt
     3901 gaacgttctc gtgaactcag tcgcgctggt cgcgccattt cttggcagtt cagtcatgcg
     3961 cataatcgtc atgatgctcg tttacgggtc actggtgttc atcaacgtgc gcggcgtgcg
     4021 cggtggcgct ggcgcggtga cgatcgtgac gttcgccaaa cttctcccgc tgttgttgtt
     4081 cattattgcc ggcatcttct tcattcaccc ggcgaatctt acctgggcaa cgtggccggg
     4141 cagcaagtcg ttaggcgacg ccgtcatttt gctgatcttc gccttcgtcg gcatcgaagt
     4201 ggcgttgatc ccgagcggcg aagtgaaaaa tccggcgcgc acggtgccgc gggcggcgta
     4261 tctcgcgctc gtcatcacca caatcattta catcctcatc caggtcgtgg cgcagggaac
     4321 gctcggcgct gaccttgcca atcacccgga cgcgcccctc gccgaagcgg cggcaaaatt
     4381 tctcggccac gtcggccgca ccgttctgct ggccggcgcg accatttctg cgtttggatt
     4441 tgtcacgagc gatattctca gttccccgcg catgatcttc gccttcggcc gtgacggcgc
     4501 gttgccggcg ttcttcgcgc acgttcaccc gcgctatcgc tcgcccgata tcgctctcat
     4561 cacctacgcg gtgctcgcat tcgccttatc gataaccggc aggtttgagc aactggccgt
     4621 cctctccaac gtcgccgtcc tcctaatgta ttttctttgt tgcgcggcct gctgggttct
     4681 ggtgcggcag gacgttcgcg ctgacggtcg gccattgaat tttccgggga tgaacatcgt
     4741 gcccgcgctc gccatcgtcg ccatcatctg gattctcgct cacgccaccg cacgcgagtt
     4801 taccgtcacg gccattgtcc tcgcggttgc gtcactgttg tatctggttc gccggatttc
     4861 attgacgtaa tcgtcgaatg aaaatggctg gtgccggcgc gaagtgagat tacgagcgtg
     4921 gcctaacgaa ttgtgaaaag aatcctgatc ctcaccgccg ggttcggcga ggggcacaac
     4981 agcgcggcgc ggggggtgcg cgacgggctg gcccgggtgg cgcccgttca ggtggaggtc
     5041 gagctacgcg atctgtttcc acagagttac ggtgcgctca acgagctcgt tcgcaaaacc
     5101 tatctggcgg tgattaacca atggccgcat tcctggggct acgtctaccg ctggctcgac
     5161 ggtatcagga acttcgacga acgcttccag cggttcagac gcttaaaaaa gcatttcgca
     5221 caagtgctcg accggttcca gccggacgtg ctcgtctcga cattcccgcc ttatccttat
     5281 ctgctgcgcc aaatcgccgg agcggaccgc agttgcaaga atgtcgtcgt gatcaccgac
     5341 tcgattacgg tgaacgcgat ctggtaccaa tgtgacgcgg actttttcct tccatcaaac
     5401 gagcagagcg cagcggtcgt gcgcgccgcc ggagtcgcgc cggaaaaaat caaggtgttc
     5461 gggttcccag tgaatccgca gttcgccgat ctcgaacaag atcgacaatc gcgccgccaa
     5521 agtgcgcggc cacacatttt atgcgtcatt catgcgggca aaaaacgggc accggagttg
     5581 gtgcgcaaac ttctcggcct cgaaattgag ttgaccgtcg cggttgggca tgaccgacga
     5641 ctgcacgatg cgattcgcag agtcagcgac aaaggtcaga tcgagatgat cggctggaca
     5701 aacgagatgc cgcgcctgct ttgccgcagc catattctta tcggcaaagc cggtggcgcg
     5761 acggtccagg aaacaatcgc cgccggctgt ccgatgattg tgagccacgt cgtctcgggc
     5821 caggaggagg gcaacgcccg cctcatcgca gaaaccgact ccggcgtcat tgctctcgaa
     5881 accgatgaag ccgtcgcgca agttagccgc gcctttgccg acggcgcgca gcaatggcgc
     5941 gaatgggccg gcaacatagc caagctaagc cgcccccgcg ccgcgctcga catcgcccaa
     6001 ttcctattgt cgatctaaag caaaagtttc ccgcccaccg agaaaggttc ggcgaactgc
     6061 aagtgccggt gaaagaacaa ccagatcagc gtccgcgccg ctgcggagcg cgcctttcgc
     6121 ttcgaggccg atcgcgcgcg cgggattttg cgttgccatt gcgatcgctt catgcagcgg
     6181 aagatcgaga tcgtgcacga gcgtgcgcac aagatcgatc atgcgcgcgg cgctgcccgc
     6241 gagcgcggaa cgatccgcga gcagacacac gccacggtcg acaatgcaat ccttaccgta
     6301 gagcgagaag tgagaccctt gctccagtcc ggctcctgct gtcgcatctg tgacgaggca
     6361 aattccactc gatgtctttg cgcgatacaa catcttcatc aaagtcggcg acacgtgatg
     6421 gccgtcggcg atcaattcgc agctgatttc cggctcgctc agcgcgaatt caagcaaccc
     6481 ggctcggcga taaattccgt cgcgccgtgc ggacgacatg cagttgaagg tgtgcgtcgt
     6541 gctgcgcaag ccgcgtgcga acgccgcgcg cgcttctgcg tcggaacaat cgctgtgacc
     6601 accactcacg cttacgccgg cggcggcaaa tttcgcgatc gcgtccagcg caccaggtaa
     6661 ctcaggcgcg atcgtgaatc gtttgattac atccacatgc tccagcaagc gagccatcgc
     6721 cgccggcgtg ggatgttgaa tgaattcggc gcgttgcgcc ccggccttcg ttttcgagat
     6781 aaagggaccc tcaacgtgaa cgccggcaat ctgcagattg gaaaatcgac aatcgcgaac
     6841 ggcgtccaag gcattgacaa tcgcgtcgag cggcgcggac gccgttgtca gcagcagtga
     6901 ggtagtcccg ccgctggcgt ggaaatcgca gatcgcgcgg aacgcgtctg cggaagcttc
     6961 cattgtgtca cgcccgaccg cgccatggag atgaagatcg atgaagcctg gcgcgaggta
     7021 atcaccgcca aggtcgacaa tctcgtcatc ggcgaagcct gtttgtggcc gaatctcggc
     7081 gattttgccg tccctgacga ccaactcgag gccgtcacgg atgccctccg gaaagatgca
     7141 gcgcccgttg cgcaggatca tgccggaaga cgattgcgtt tgcgaacgcg aaagtccatt
     7201 ttcgagagcg gctcatggac ttcgattacg atgtcgtgat cattggcggc gcgttttccg
     7261 gcgcagctac ggccctgatg ctcaagcgca aacagccgaa tgcgcgcgtg ctcatcgttg
     7321 aaaaaaacgc ggagttcgac cggaaagtcg gtgaatcgac caccgaggtg agcagttgct
     7381 acatgacgcg catccttggc ctaacgaatt atctcgggca cgagcacctg gccaaacaag
     7441 gtttgcgtct ctggttttac aattccccgg agcaaaggtt tgatgactgc gtcgaaatcg
     7501 ggccgcacta caacgcgcgc gtgcagacgt tccaggtcga tcgcgcgaag ctcgactcgc
     7561 acttgctcga atgcgccgta aatgccgact gcgacctctg gcgtccggca aaagtggcgc
     7621 ggatcgattt gaacgcggcg aacggccaga cgatcactgt ggatgtcgat cttgcacaac
     7681 gcgccgttcg tacgcgttgg gtaatcgatg ccagcggccg cgccaccctg cttgcgcgca
     7741 agctcggaca ttttcggcct aacgcggagc acccgatcaa cgcggtctgg gcccgattca
     7801 ccggagtgaa agactgggac agctacgatt ggcgcgaaaa atttcgcgat tacatggacg
     7861 aatgccgcac cgcgcgctca tgggcgacca accatctcat gggccatggc tggtggtgct
     7921 ggatcatccc tctgcgcggc ggtgatgtga gcgcgggcat cgtttacgat aatcgcatct
     7981 tcaaattgcc ggacggcacg aatctcgccg ggcgattgca cgcgcacctc gtcggccatc
     8041 cggtcggtcg cgaaattttc cacgaggcgc gtgcgatcga gggagatgtg catgcgctgt
     8101 cgttgttgcc ctatcatagc gaaagagtct gcggcgaggg ctgggcgctg gtcggcgacg
     8161 cggctggttt catcgatccg ctttacagcc ccggtctcga tttttgttcc tacacgagca
     8221 actatgtggc ggatttcctc gcgcgcagtc tagccgggga ggacgtgagc gaatcggtcc
     8281 ggcattacaa cgagcagtac cccatcatgt accgctcgtg gttcaaaacc ctctacaagg
     8341 acaagtatta ttacatgggc gacgccgaat tgatgtcggc cgcgttgctg ctcgacgtgg
     8401 cctcctatta tttcggcctg gtcattccag cctacaagga tccggagaaa gcgtttctgc
     8461 agctgccgtt tcgcggaacg ccgggacgaa ttgcggcacg catgatcagc ttctacaatc
     8521 ggcgactcgt taggctggcg cggcgcaggc tcgccacggg ttatttcggc aaaaacaacg
     8581 ccggctggcg cgagctttac gacggattcg tgccggactg gcgagcgacc aagctgctgc
     8641 gcaaggggct gtttcgctgg tggaaagcgg agctcacaaa tttgaggcta atattcgttt
     8701 caccggcgcg agtaaggccg aatgagagct ctgcgccagc acctgccgaa gcctgagcaa
     8761 aagcgccgcg cggacaaagc ttgtcgtcgc gacgttcaag aggggctgag caagccgaga
     8821 taccaccgca caaacgccgg gccatagaac atccaggtca ctgcgccgaa agccagatac
     8881 ggcccgaagg ggagcttcgc cgaccagaca cgtttcccga caagtaaggt gaacaagccg
     8941 acaagggagc cgagaagtga tcccgcaaaa accgcgaaaa gcacggcccg ccagcctagg
     9001 aacgccccga tcgccgcgag aaattttaag tcgccgcggc ccatcgcttc gcgaggaata
     9061 acgatcagga cggccgtgcc ggtgatttcg ttaacgtgat ctagtagcaa cgtttctcct
     9121 tccacgacca ggcggtcgta gtaaaactcc agggtgattt tattgtgaat gcgattgtcg
     9181 atcttcgcgt catcgcattc caggattagc cggtcttttt cgcgagcgaa gtaatcgctc
     9241 cacaaacttt gctccgcgcc gacaacgaat tcggcatcat cgccctggcg cttccacgtg
     9301 aatggcgtcg ggccgtcgag tttgattcgt tttttcccga acgccagttt gcctgcctcc
     9361 aaaacgaaca gcaggacgag ataaccgaga gcggccgcga gcatggagcg aatgagcgcg
     9421 gcgagccgcg aatcggtctc cattaactgc ggaacggcca gactcgcgag cacgcccatt
     9481 atcgcgccgc caatcgtgac ctggtcagga atgatgaagt gctcaaagtc gacaaaggtc
     9541 gccacgatga cgagcgaaac gaaaatccag taggcgatcg ccatcgggat gggaaagctt
     9601 tgccagatgg cgagaaacaa aaacgcggtg agcaattcca cggcaaaata acggaggctg
     9661 atgctggcgc cgcagttcgc gcagcgaccg cgcagcaaca tccaactggc gagcggcaga
     9721 ttgtgctgcc agggaatggg atatttgcag gccgggcaga aagatcggcg cggtcgattg
     9781 atcgaaagat ctagcggcaa gcgataaata caaacattga ggaacgatcc gacgacggcg
     9841 ccggtgatga aaacaaaaat gctgaaaaga agccggtagg ccgagatatc cacgcgctca
     9901 ggcaacgttg cgttgcaacg cgcggcgttc catcagccac gcaatccaga tacagctttc
     9961 gtagaggaga cacatcggca gtcccatcgc gatcagtgtc aggatatccg gcgtgggggt
    10021 gataatggtg gcgagaataa agatgaggac gatggcgtac ggtcgggtgc gcgccatgaa
    10081 tcgataggtg accaacccaa agcgcacgag cgccagcacc acaaccggca attcaaaagc
    10141 aagcccgaag ccgattgtga agcgcgtcac aaaagaataa tactgctgca ccgtccaagt
    10201 cggcgtccaa ccgagcgatt gcgtatcgcg aaaaaagaag aggatcgttt tgggcaaaag
    10261 ccagaaataa cagagcagca caccgagcaa aaataaggcg aagctgaccg tgatcgccgg
    10321 aagcagaaaa cgtttttcca cggcggtgag cgccggcaaa acgaactccg cgcagaaata
    10381 gaggagcaac gggaatgaga gaacgatccc agcgtagaac gcgagatgaa acgaaatcac
    10441 gatcgaatcg gttatgccga gggcacgcaa ctcggcggct tgttcgccga tctcgcgcag
    10501 cggcgcctgc aggatgcgga cgagcgtcga gcgaaaaccg aaacagacga tcatcgccgc
    10561 gatgagcgtg atcgccatct tcacaatggt ccagcggaga tcctcgagat gctcgagaaa
    10621 cggtttcgag gtctcgccct ctggcagctc gcgaaattta aaaatgtccc gcagcggcat
    10681 tagaatcgac agtcactcgg caggcgccag cgaaatgaag ccgtgcgccg cgagccgggc
    10741 gcaaatcccc agcgtgttgg catcgcgaat ctcgttatca gcaatcatct ggcggatttc
    10801 gcccactgtg aacgcgtggc agtcgagaat cgattcccct tcaccgtgac cgtctccgcg
    10861 tgcgctatgc ttgactggcc gtgccagaaa gagatagccg tactcgtccg taaaaccggg
    10921 tgaagcgaaa taatggccga gcgcaatcag ttcgccgccg ggagcgagtt cgtacccggt
    10981 ctcttcgcgc aactcgcgaa gtgccacttc tctgatcgcc tcgtcgtcgg cttcgccgtt
    11041 atcaatttgc ccagccggca tctcccaaat tgcagcgcga atcggcacgc gttcctgccg
    11101 gatgagcagc aatttcccgt cgatcgtcat tggcgcgatg attacggccg ctttccgatg
    11161 caccaccgtc caggggcgtg gctgcgagcg catgggcgtg cgcacaatat cggtgacaac
    11221 ttcaaggtgc ttgttggtga aatgatgctc gctcgacacg gtttcccacc caccggtgtc
    11281 ctgcacaatg ttgctccgcg tcacgcggcc actgtagagg ccgctctcct cagcggcaaa
    11341 ccgtgttacg tcagctcggc gcgccagcgt ttgatctgcg cagcaacgtt ctcgggcgac
    11401 ggcgcgccga tcgcgtggcg ttgtgcaagc gagtggcgcg gatcgaacac gcgcgcaaga
    11461 tcggtttcaa acaatggaga aaacttgcgc aactcctcca gcggcatctc gttgagcggc
    11521 accttcgact gactcgcttg cgcgacgatt cggccgatga tctcgtgggc ttcgcgaaat
    11581 ggcgcacctt ttctgaccag atattcggcc acatccgtgg ctagcaaatt tggatcggcg
    11641 gtcgcagctt ccatcccttc gcgattgatc ttcagttctc gcaatgcatc ggcgaagact
    11701 tccagcgcca ggttgattgt gtcgctggaa tcgaataacg cgtgcttgtc ttcctgcagg
    11761 tcacggttgt agccggcggg gagcgctttc agcgtggtta acatcgacat gagattgccg
    11821 aaaagccggc cggttttgcc gcgcgtgagc tcagtgacat ccgggttttt cttttgcggc
    11881 attaaactcg agccgctcga aaatgcgtcg ctgaagtcga cgaaaccgaa ttcggtcgtg
    11941 ctccagagga tcaggtcctc gctcaagcgc gagagatgca gaccgatcat ggccaaacaa
    12001 aacagaaatt cgcagatgaa atcgcgctca ctgacggcgt cgagactgtt ctgtgacaca
    12061 cgcgcgaacc cgagctcgcg cgcgatgaat tgtcgatcta acacaatcgt cgatccggcg
    12121 agagcacctg acccgagcgg aagaacatcg gtgtgtccaa ggcaagcacg caggcgctca
    12181 cggtcgcgct cgaacgcttc gatttgcgcg agtaaatagt gggcgaacga gatcggctgt
    12241 gctcgttgca tatgtgtgta accaggcatg acaatgctga catgttcctc ggccaggtct
    12301 aacagtgtgg cctcgagcga acgcaatcga ttgtcgatgt tcgcgatctc cgttttgacg
    12361 tagagccgca gatcgagggc gatctggtcg ttgcggctgc gggcagtatg aagctttgct
    12421 ccgactgcgc cgattttgtg ggtaagcgcg gcctcgatat tcatgtgcac gtcttcgagc
    12481 gcgacatcca tttgcactcg cctcgttcga tttcgctcgc gatctcgcgc aaaccagtct
    12541 cgattttttc ccgctcatcg gccgaaatga ttcccgccgc agccagcgca cgggcgtgag
    12601 taattgagcc aaggatgtcc tcgtgataca gccgccagtc gaacgagaca gattcggtgt
    12661 agcgaaccgc cgaatcggcg agcggagcgg ccagtctgcc tttgcgcatc ttaatcctct
    12721 gcgttgcggt cgacgaagac agcggcgtag cgcgcatcag cgagaacgaa cttgcgtagc
    12781 tcaacgctga cctgccgggc gggaaaggtg cgcaacaggt ggtcagcgat ttgttcggct
    12841 aacgtttcga tcagtttcgc gctctctgcg ccggccactc gtcttgtttc ttcgcacacg
    12901 gccgagtagt cgatcgtgcg tgtgatgtcc tcgctgagat cgcgaaattt cgctcgaggc
    12961 cagagcgtaa tgttcaccgc gagccgttgt ggctgagcgc gctcctcgtt cgaaacgccc
    13021 acccgcgcaa aaatctccaa ctcctcgata tgaattttgt cgccggctga gccgttcatt
    13081 tgcccaatca acatccgaga ccgcagtcgg ggaactgaaa aaaatcggtt cgcaggaaac
    13141 ttccggccgg atttcccgta ggtgcaggta tgaggaaatg gatacttcgg gacctgccgc
    13201 gctcgctgga gaggaagctg cggcactggt tggagcgatt gtttgggcga aggccgcagc
    13261 gaccagttcg gccgcaggtc cgcggacgca aatgaacgct catctcaagt cgccgcagca
    13321 aaagcatgag gaaggcagtt ctgctttagg cttgtgcgcg aattgcggga ataacatgat
    13381 cgtcgccggt gaacgcgaat gccgcgctca aaggcgaccg gcagaacggt ttcgtgacca
    13441 cgcgctggag cgtggtgctc tcatgcggca gcccggaaga gcgcggcagt gcggcattgg
    13501 ccgagctgtg ccgcatctac tggcgcccca ttttcgcttt catttgccgt cgtggctact
    13561 ccgtcacgga cgctcaggat ttgacacaag acttctttct catggtgatt gaaggcaatc
    13621 ttctccagct ggccgatccg tgccgcggac gctttcgctc cctcctcctg aaagcgatgc
    13681 aaaatttctt gatcgacagt cacgcgtggc gcaaggcgca aaagcgcggc gggaaaatcc
    13741 aatttgtctg ctggaatgag tggatggccg aagcgccgtc acaattgtcg attcacacac
    13801 gcgaagcgga aagctggccg gcggaaaaaa tttatgatgt ccgctgggct gcgactgtgg
    13861 tggagcgagc actgcaccgt ctcggcgaag aatgcgaaag ccgcggccgc cggcgcatgt
    13921 tcagcgtgtt aagcaaatat ctcgccgcgg aacggagcga cgtttcgtat gttgcgatcg
    13981 gcaacgatct cggcgtctcg gccgacgcaa tcaaacggtt gttgcatcga ctgcgtgata
    14041 ggtaccgcgc cattctccgt gaggaagttt tgcagaccgt cgaaagcgcg gcggaagtag
    14101 atgaggagat ccgttatctc tgcgccgtcc tcgcgaccga agaaccgccg cacgcgcaat
    14161 gaatatcgcc ggcctttctg atttcaccgg taacgcctct gacgaagcgc caaagtgcga
    14221 gtgcggatcg gcggcgacag tcagcgttgg gctgtgcgtg agctgtctgc tgcgctgcgg
    14281 gttgcgggca gaggcagcaa ctgaggaaag ctttgctgaa gcgctgcggc aaatcgagat
    14341 ccgcgatagt gactggcagc tgggaaatta ccagatcctc gaagaaatcg gccgcggcgg
    14401 aatgggtgta atttatcgtg cgcgacagcg gcattccgga cgtatcgtcg cgctcaaacg
    14461 cgtactgagt tatcacagcg actcgcacga gacgctaacg cgatttcgcc gggaggcaga
    14521 agcggcggcg agcttggacc acgcaaacat cctgccgatt tatgaggtgg cgatgggtga
    14581 ggacgggttg ccgtttttca gtatgaaatt cgccccgggc ggaagcctgt tgcccgcgcg
    14641 gacgacctat cgcgcccggc cgcgccaagc cgtgcagctg ctggcaaagg tcgcacgtgc
    14701 aattgaacac gcgcacacgg ctggcattct ccaccgagat ctcaaaccgg gaaacatcct
    14761 gcttgacgct cgcggcgaac cgctcgtgag cgatttcggt ctggcgaaat ggctcgacgc
    14821 cacgagtgat ctcacacgaa cgatgacggt attcggcacg ccgggataca tcgcgccgga
    14881 acaagtgcgt tcccctgccg aaatgttgac ggcagctgtt gatgtttaca gcctcggcgc
    14941 cattctcttt gagctgctca gtggccggcc tccgtttgta gcggagcatg ctattgccgt
    15001 gattcatcag gcagccgaga aacaagcgcc caggttgcgc tcggttgcgc cggcactctc
    15061 gcgcgatttg gaaacaatct gcgcccgttg tttggaacgt gacccgcggg cgcgttatca
    15121 atccgcgggc gttctcgccg aggatctgga acgctggctc gaagatcgtc cgattagcgt
    15181 gcggccagtc tgcgcgcccg ttcggctttg gcgctggtcg cgccggaatc ggcgtctcgc
    15241 cgtggcttgt gcagcgtgtg tgctactcgg cgcggttgcg gcgctacgcc agctccaaag
    15301 ttggcatttg gaatcggcgg tacgcaaagc agtgttagct gcgcattcga tcgccgttct
    15361 cccgtttcta aatctcgaca ccggcgaaat ggcgaatgac atgacggcgc gtttgggcaa
    15421 ggcgctgcgc gccgaacttc cggctttggg tccggccaag attgtggagc tgccgatccc
    15481 atctgcgaaa tggacgggaa cgggaaatgt cgatgaaatc cgaatcgcgg ccgggcaggc
    15541 agatacgcgg gcagttcttt ccggcacagt gcggcacagt ggtcgcggtg ttcggctgtc
    15601 gatccatctc tttcgcgaga ccggggatga cacgcttggt cagtggttgc tggatgtcgg
    15661 tgctatcgag gagctgccac aggcgctccg gaggcccggc ggtatcgccg ctctgcttta
    15721 ccgtgcgctc gatgcgagga cgcatgcgca gaagggcgcc agcaacaccg acgccgcaat
    15781 ggtagacgat gtcgcgcgcg gctattttaa cgccggacgt gcattgctgg atcggcggac
    15841 cattccagat atggatcgcg cgattaattg tttcgaagaa gcagtgcggg ccgctcccgc
    15901 ttccgtcgcg gcgcgttcct atctcgcatt ggcttatctg ggacgcaatt ttctcaccgc
    15961 taatccggcg catatggaac gcgcgttcgc ggtcgcaaac caggcgctcg cgcttgactc
    16021 ccacaacccc gctccgcacc gcgcattgtg ctcgctctat acttccacag ggcaattcga
    16081 gcaggcgctg gaacatgggc tactcgcgtt ggaggcaggc gacccgagcg aacgcgcgct
    16141 cgggcaaatc gctttcgtct ggaaggaatt ggggcatccc gaaaaggcaa tccgctggtt
    16201 tggcaaagcc aaactgagcg agcgacagcc ggccgactac gatgcattgc tcggcgactg
    16261 ctggatgctt ttgaccgaag acgatcaggc gcgagaggca taccgcgcgg cagcgaattt
    16321 tcaccccgat ttacccgagg gatggcttgg cctctgtcac cttcagcttc taaacggcga
    16381 ttttgctggc gcacgcatgc ttttccggca acgcgcgggt gagtatcagg cctttcacac
    16441 ggtcaaacca ttactcgcgc agatcgaatt cttttcccgt aatttcgctg aagcagagcg
    16501 actctaccgt gaaatccggg acgccgatcc gcgcggcgtt ggcttggagc aatacggtgc
    16561 aatcagtagc gcatcggctc tcgcccggtt gaaatttgcc accggcgatc tcgtctctgg
    16621 caatcagctt ctcaagcaat gcctggcgag cgatcaagcg caagtccgag tctcgccgca
    16681 caacgccgag gtgctctacc gcctctctgc cgacgaggcg atacaaggga acgtcgccat
    16741 gagcctgcaa tttttacgca acagcatcgc cgctgggtgg ctcgattacc ggtcgacccg
    16801 ccttgatccg cgcttcgacc aagtagcggc caaccccgaa ttccaaaagc ttctctccga
    16861 gctggccgcg caagtggctg gccttcggac ggcactcacc agccgatctc ccaaaggtga
    16921 aaatgaaaac tcagcgaggt aaacaacatg ccaagaacat ggaaatccaa acaggaccgc
    16981 agagacgccg ctgtcgcgtt tctccggaaa acgatcactg atccagatgt gcgttccgcc
    17041 gttctcaaag atcgacaatt agcgcacaag ctctttgagc gcgaaggcga cattaacatc
    17101 ccagacgacg tggaagtgat ttgcgttgga ccgagcacgc aggagcgcga tcgactcgtc
    17161 gtgttcgttc tgccaccgga gggcaccgac accgaacatc tcgatccgtt caagtactgg
    17221 accggcacct ggctcccgta caacatggat ccgatgaagg tcgtcgcgtc tgcggagcgc
    17281 accggcgccc gcgcgcccga acctgtcgcc gcgtcataag cggtcagccg ttcggaaaaa
    17341 ttctgagagc ccgtagagtc ggacgtcttc ctctgcgaac ctccagactg gcgggaagtc
    17401 atcggcgccg acccgattca gataagaatt ggggtcggcg cgaagggccg catacagcgt
    17461 ttcggcaatg acgcggctgc cgagcggccc gactcggttg ccgttgtgtc gcacctcgga
    17521 ttctttgagg atataatacc acagcggcgt gtcgcgcgtg aggccagtat cacggagaat
    17581 ctggccctgc gccgtttctt ctccctcgag gttctgcgtc agttccgctt cgcttaatac
    17641 gcgctcgtgc aaggcgcgcg ccgcgctctg cccggatgcc aaccgcaagc cggcgccacg
    17701 caacaaggtt cgcaccggca gttccgccgg gtttttcgcg accctgaagt gagggcaggc
    17761 aatttgcgcg acgccaacta aatcgtccgg caacaactga aacgacggcg ccagacgcgt
    17821 gtcgatcggg cggcttgtca ccgagcggcc atcgcctgcg ccttgaaaaa agaatcccca
    17881 gttgatttcc accttctcgt caagcggccc gcgctccgaa gttcggctga agatttgcgg
    17941 cagacgcatt tcctgcccga aagagaatag ataatttggt cgcaccatcg cgtgcccaaa
    18001 acgcatggcc gccgcagcga actcgatcgg cacggaaaac gtattccatc tgattatcga
    18061 tcttccgtgg acaaaaactt ccctgtagac agccgggtcg agcagcgtcg gaagataatc
    18121 gcggcagacg agccactggt attgccattg cgtctggagc cgcgctcgcg cgaacagcgc
    18181 tgtgttgtcc gcaaattcgc cttgaaattg ctcgacggcg aaattgtgca ctcgtgcaaa
    18241 aacagccgtc atttgccgca ggatcagatt ttctgtgttg cgctcatcgg ctagaacgca
    18301 cccgccattc cccgcaacgt gaatgtcgaa tgagtgtcga ccgtttttcc gttggccgac
    18361 cttcaaccgc actccgtcag tttcataaag ctccggtgaa atctcagggc cgccgccgta
    18421 taatgtgctg aggtccagct tcggcgtctg caggttctcc agctcctccg gttctttttg
    18481 ccacgcgccg tcgaccgagc tgacatcctt cgtcagatcg tgcgcgatga attggccaaa
    18541 atacgtgtag ccgctgtcga cgagaatttc cgggcgctcg tcggaaacgc gggtgccgtc
    18601 gtcgatcatt gcgcgaccga gttcggtaag cgcttgacaa tattgatcgg catccgcccg
    18661 caaccgcatc ggccggaaga gacggcagta gcgatcgagc gccgattcgc gaaacggtct
    18721 gagataatcg tccactccgc cccggttcta cggagtcggc gggacgaagt tcctgaatta
    18781 ggtcagccga cgcgaaaacg gacgtccccg acgactttac cgccgaagcg ccgcttcagt
    18841 tttcgcaaga tgtcgatctt ggacacctgt tcgagctcgt agtgcaacgc cggttgcaat
    18901 acgcggacgt agaggacgcc gtcgtgcaaa gccaccggcg tcgaatgggc ggcgatgaac
    18961 tcaccgacga tgttgctcca ggcgtccata atctccgatt cctgcaagcg ctccttcaga
    19021 ccgagccgtt gcatcatctt cggcagcagc tcggccgtcg cttgccaccg gtccggttca
    19081 acacggtgct ccggtgagcc gcgccattcg gcaacggcgc ggttccgcag ggtcgagttc
    19141 acattacaag agcgacaatc aatcagtatt tccgccgcat gttgctcggc agaatattca
    19201 actcagtgcg atatttcgcg acggtgcggc gagcaatagg gatcccgcgt ttgctgagga
    19261 tttcgacgat ctccttatcg ctaagcggcg cgttgccgtc ctcgtttttg acaagatcga
    19321 ggatcgcttc tttgacgctc gtgttgctca tcgattcgcc gctcgaagtc tgataacccg
    19381 gcgtgaagaa gtatttcatt tcgaatacac cctgcggcgt cgcgatgtat ttgccggaga
    19441 tagcccggct cacggttgtc tcgtgcacgc cgaccgcgtc ggcaatttgc accattgtca
    19501 tcggtttgag gtgtgaggag ccgtgctcaa agaattcgcg ctgccgttgc acgatctggt
    19561 gagcaatgtt cgagatcgtc tgctggcgtt ggtgaatgga cttaatcaga aatttgccgc
    19621 tccggatttt gtcgcggatg taatctttga cttcgttgcc gttcccctcc tgcgccatga
    19681 tgtctttgta ggtgttgctg atgcgcaggt gcgggatttg ttcgccgttc aagattacct
    19741 ggtactggcc gttcactttc tcgacggtta cgtccggcag gacataattt tgcggtgagg
    19801 cggcgaagat ttggccaggt cgcggatcga gctgcgcgat attattcgcg cacttctgca
    19861 cctgctcgac gctgatgccc atacggcgcg caatctcggg aaaacgccgc ttcccgagat
    19921 cctgcatgtg ctccgatacc accttgtact cgaggctgtt ttgccggcct tcgcgcttga
    19981 gttgaatgag caagcactcg cgcaaatcgc gcgcgcctac cccgggcgga taaaaactct
    20041 ggatcagcgt cagcatgttt tcgaaatcgg cctgcgggat accggtgttg agcgccattt
    20101 cctcgggcgt ggtttgcaga aaaccgttgt catcaatgtt gccgatgatg agctcggccg
    20161 tcttgcgatc gtttgtgttc agcacggttt ggtttagttg ccccatgaga tgctgttgca
    20221 gcgtctcttg cgtcgcgatc gaatcgaaaa agaactgccg cttgtcctcg gcttcctggg
    20281 aacggccggt gtagctgctc gattgcgcca tgtaatcgcg ccattcctcg tcgagcttgg
    20341 ccagcttatc gaattcctct ttgaacttcg cgtctgcggc gggatcggct tccgtgcgtg
    20401 gttcggcatc gggatcttcg ggcatttctt ccagtaccgg gttcacttcc atctcctgct
    20461 gcacgagatt gcgcagctca agcagcggcg cctgcaggat gagtaagctc tgttgcagtt
    20521 gcggcgccag gacctgctgc aaggacaaat tctgcgtctg gtgcattcct ggcccggcca
    20581 tacgcgagct tagcgattac ggcgagcaga ggcaagcaga ttacaggcca gacctgcgct
    20641 ctttccctcg tgtacgagcg ccgcaacgcg ttacggaaca cgacttgctc tgagggttcg
    20701 gcacaggctt ctcacaatga aacgtccgct cttcgcgctc cttctcattt tcttcggagc
    20761 tctctcgact ccggcgcaaa tgccgaaaaa ggacgatccg caacagctgc tcgcgttggt
    20821 caaagaggtg caggcacagc aactgcaaat cgccgccaat caggcgaaga tcgaagccaa
    20881 gctcgctgag ctctcggaaa cgatccgcgt cgcacggatt tattccagcc gcagccgtta
    20941 agcctcgtga aagcaccgct tctcttgctg tcgatcttcg ctgccgtcgc aatctttacg
    21001 gccgacgcgc aatcaccgac acccatgcag ccggtcacca ccgacaccga gtcaatcccc
    21061 ggcgcgatca aagtgcttca ggagatgaaa acggcgaacg acgaagtgtt gaagaaacag
    21121 gaagcgactt tgcaggcgtt ggaagagctc cagaaggccg ccgacgaaat caagatcttc
    21181 ggcaagcgca gctaagatcg acaatcgcca agctcgagat gcgggtcaca gcccagccat
    21241 gtcccacttt ctaatcgtct cctcaatcaa ctgcgcgatt ttgtcgttta ctttggcgaa
    21301 gtagctcttc gctttgactg aatcgaaccg cgggtaaccc tggctttggt tgtagagcat
    21361 gatgctcgat ggaaacgggt acgcgctcca agtgttcggg gctggcatgg ctgtgaccat
    21421 gtcacggtta aacctttttt catccaccag cgccggatca atcgccatga cataggcagt
    21481 ttcgttgtcg ccggcgtgac cgccgtcttc gccaaatact tcaagcgtga catcgctgca
    21541 atacgaccac cagttcacta gcagaatgcg tgtgcccgtt tcacgtccga cctcttgggc
    21601 aagtgctgcc aaaattgcgg tctgcccacc gccgtggccg ttgagtaaaa tgagattttt
    21661 gaatttgttt ttcgcgagac ccgtcatcac tgcacggacg tactcgcggt aaggcgcttc
    21721 aggaatggtg aagctgccgg gataagcgtc cagtacgcca gtgaacccgt acgcaatggc
    21781 cggcgcgatc atcgcgttga cgcgcggcgc aatctcacgc gccatcgcga cgggggcaat
    21841 gatgtccgtg ccgttggcag tcacgccgtg tgcctcgagc gtgcccagcg gcagcaacac
    21901 agttttaatt tttgccggca cccactcgtc gaattcgagc cagttgatgc gctccatttc
    21961 ccgtgtactc gggccggaat ctttcatttc gatcttgctc aacgaaccat ctgtagccac
    22021 ggccctgcgg gccgtcttac gtagaggcgc cacagccgag tggctacggc aatttcactg
    22081 caacgcggcg ctcgtcaccg tttactcttc ggttcgatct tccgataaag cgtggccatg
    22141 ctgatgtcga gcatgctggc ggctttttcg cgcgaaccgc cgcattgcgc gagggtcgct
    22201 tcaatgtaat tacgttcctg ttgctggatg aattcgtcca ggggctggcc gaccggcatc
    22261 gccgcgatcg cgccgtcgct tttcccgcct tgcgcctcgc tcacgatgtg cggcggcaga
    22321 tcgctcggca aaatcagctc gttctcgcac aacgcgcagg cccgctcaat cgcgttctcc
    22381 agctcgcgca cgttgcccgg atacgagtag tgagacaaaa tatcgatcgc cgccggatcg
    22441 atcttcttcg gttcgcctcc tgacgcgctc gcctgttttt gcaggaaatg gtgaaccagc
    22501 agtggcacgt cttccaagcg ctcacgcaac gctgggatct ctaccggaat cacggccagc
    22561 cggtaataaa gatcttcacg gaaggtgccg tccttcagtt tgggcaagag cggctcgttc
    22621 gtcgcgccga ccacgcgcac gttcaccggc gagcttttgt tgtcgccgac tcgccgaacc
    22681 tcacgttcct gcagcacgcg cagcagtttc gtctgcaacg gcggcgccat cgagttcact
    22741 tcgtcgagga aaatcgtccc gagatttgcc tcttcgaaca agccgcgctt gtccgcgacc
    22801 gcgcctgtga atgcgccctt tttgtgaccg aacaattccg attcgagcag gttctcgggt
    22861 aaagcactgc aattaatcgc gacaaaagga tgatgctgcc ggttgctgtt gaaatgcagg
    22921 ccgcgcgcga ccagctcctt gcccgtgccg ctttcaccga cgatcagcac cgtgctgtcc
    22981 gtgtcggcga ctttattgat taggttgtag acctgctgca tcttttggct ggtgccgatg
    23041 atgttctcga atcggtaacg gttcttaagt tccttgcgga gatagacgtt ctcccgtagc
    23101 gccgcttgat aatcgagcgc gcgctgcacg gtgagttgca gctcgtcgat cttgaacggc
    23161 ttggtcacat aatcatacgc gcccatcttc atcgcctcga ccgccgtctc gaccgttccg
    23221 taggcggtga tcaaaatgac cgccgtttgc cggtgatgtt cccgcacatg ccgtaagaca
    23281 tcgaagccgg tcatcgtttc catcttgatg tcggcgatca ccacttgcgg cgcgaggctg
    23341 tcaagctgct cgaccgcttt cggcgcggaa ttaaatggaa aaacctggtg gcccttctcg
    23401 cgacaaagcg tcacgatgac ctccaccatc gcgggttcgt catcaatgac gagaattttg
    23461 gccataaaat ctcagcgcgg agaatatttc aggtttgaga aaagaaaagt ggcttttgag
    23521 aatctacggg acgtgaccgg aaacacagcc gtccatgacc gtcccctcga cagcgccgtc
    23581 aaatgcgaca atctcctcgt aagccgctgc cggcttcgtg cacggaacag ctattatgtc
    23641 agcgcgaaat ccggcaagaa gccgtgccaa cgcacccttg tgctgtaacg cgcgcgcggg
    23701 gttcaccgtc accatctgca gaatctcttc cggcgaaacg tttggaaatt ctttttgaaa
    23761 cgcccgcatc tccgcaaata ggctgagatt gtcattactc gcgagactgt cggttccgag
    23821 acagatgttg aatccgagcg cgcgcaaccg ctcgaactga aacggcgagt ggccgaagta
    23881 ggaatggcta cgcggacaat gcacaatcga aaaattcctg ggtgattttt ccaacaaatt
    23941 ggaatcgctg tcggtgagtt cattcagatg aacaaccaac cactcctttg tcatgccgag
    24001 cggagtcgac aaatctctga aggttaaaaa ccaagcgagc ggcgtcttac cgccgcaatc
    24061 actcatgtca cgacccagct ccttcaggaa atcgtagagc ggcccgctcg cgtcgcggaa
    24121 catcgacatt tcttcgtgtg actcggcgat atgcgtcgtc agcaatgacg ccagccgttc
    24181 acagcgtcgg tacaactctg ccgacgccgt gaatggcgcg tgtggcgcca gcccccagtg
    24241 ctccgctgat cgtagaaatt tcgccgccag atcggcatct gcggtgccgc ggacgtcgat
    24301 cagttcgcca aaccaacacg tacgaatcgg aaaggaaatc tgcgggacca gctccggaaa
    24361 ggccgtcaga ttggccatcg tcgtggtccc gaaacatttc acttccgtga aaccgttatt
    24421 gatcgaagcg acgtaatctt ccggcgcgag cttcgccttc tccgcgttga tcgcgcgaat
    24481 ccaatcggcg aacgatttct gcc
//
LOCUS       DASTDG010000227         9649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_156970, whole genome shotgun sequence.
ACCESSION   DASTDG010000227 DASTDG010000000
VERSION     DASTDG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156970"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFO30_07570"
     CDS             <1..351
                     /locus_tag="VFO30_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HET9419183.1"
                     /translation="VARSVLDFFNSVAGKKILRRMAELEIEPKSEKLSAKKAATLPLA
                     GKTFVLTGTLPSMTREEATEKIESLGGHVAGSVSRKTDYVLAGAEPGSKIDKAKELGV
                     RVIDEAEFRNVLGA"
     gene            complement(366..1409)
                     /locus_tag="VFO30_07575"
     CDS             complement(366..1409)
                     /locus_tag="VFO30_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9419184.1"
                     /translation="MRAQFRWLNTAIFVATLCAGSQLLLAQEKAPESISREVKGIFAG
                     AARAVVKIHGVDEHSEIAGTGFFVDPTGALYTSYTVGGEAGDFEIEFDGQKYPARQLL
                     ADIRSGIALLKVDLATPALPIGRSDQLEIATPVVSIGYPLDLPETPSFGMIAGFDRKY
                     LGRYFSTTHLRVNLPTQRGEAGAPLLNLKGEVIGIIVSSLENNSACYALPIDAAEKIR
                     GDYMRFGEVRHGWVGINVSEAETPVEGSRAEMTKIMNGTPAAKSGIQAGDILLQVGNK
                     KVREPEDVLDASFFITAGETVPITVRRGNDKLTFNVQADLHPASQRPPLIVAPSMNQA
                     IPLKLDAQVERRP"
     gene            complement(1429..1923)
                     /locus_tag="VFO30_07580"
     CDS             complement(1429..1923)
                     /locus_tag="VFO30_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419185.1"
                     /translation="MEEFDDKEIATLLRLKRYEQPPPGYFEDFLHEFHRRQRDALLCE
                     PLWRICLHRTHDFFLRLNVFGSTGSYATAAAAVLVCAAVISLKMYQPSPPASVAARAD
                     TSWNAPANLGAEWTLASPVAERLFSAKPLRKLRAQTHSHDRGTPPRYVLDSVPVSYET
                     TVRF"
     gene            complement(1932..2528)
                     /locus_tag="VFO30_07585"
     CDS             complement(1932..2528)
                     /locus_tag="VFO30_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9419186.1"
                     /translation="MDRPKSDEDQELVARTQAGDASAFDQLVVKYSPRLYGLVYNMTA
                     NHEDTNDLLQDVFAKAYKAIRGFRGKSSFYTWIHSIAVNMTLNFLKKRGRRFHMSLDD
                     VDSSVQSDKQLLQLTAKSSPVREADLSELQRRLNEAMMKLSDEHRAVVTMFHIQGMPH
                     AEISKILKVSEGTVRSRLFYANRQLQSYLDEFRKNPVT"
     gene            complement(2622..3320)
                     /locus_tag="VFO30_07590"
     CDS             complement(2622..3320)
                     /locus_tag="VFO30_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET9419187.1"
                     /translation="MFYPYGFGFGGHWLIYIGIPLVIGLWAQIRVSSAFSRWGKVRAT
                     SNITGAECAREILQAARIDDVDVVETNDFLGDHYDPTRKKLCLSSNVYRTPSVAALGI
                     AAHETGHAIQHAKAYAPLKARMAIVPVTMVASQMLPFVIFGGLFFGFTGLITLGIYCY
                     MILLAFQLITLPVEFDASRRAKIILREMGIVQSGQEVAGVNNVLNAAALTYVAAFIAA
                     LGNLLWLMSIRDRR"
     gene            complement(3492..4625)
                     /locus_tag="VFO30_07595"
     CDS             complement(3492..4625)
                     /locus_tag="VFO30_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9419188.1"
                     /translation="MSKTKPNGTRLIVAASDQDADMLYATRFWAGDTFIFLQQNGKRT
                     IVLTDLEMDRGRRTAKVDEFVSYNRLEREVQGKSKQAPPFEKVLAHFLRKRRVKRATV
                     PMNFPLGYARELAREHVDLAPTNGLFWPEREAKTDEELKLIGRALRITEAGMRRAIEV
                     LRKSTIARDKKLKWSGKVLTSEILRAEIDTALLRAGGTPTNTIVAGGDQACDPHERGS
                     GPLRANQLIILDIFPRDPRSGYWGDMTRTVVRGRATDAQRKLWHTVKEGQELALKRIK
                     AGVEGMTIHKAIEKLFADRSFPTELRDGKNVGFFHGTGHGLGLDIHEYPRLQKVKLKD
                     RQVLTVEPGLYYPGLGGVRHEDVVVVTKTGCKILSRFPKQLEI"
     gene            complement(4622..5857)
                     /locus_tag="VFO30_07600"
     CDS             complement(4622..5857)
                     /locus_tag="VFO30_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419189.1"
                     /translation="MNEVYILGGARTDFKRNLKKEGKTIRHLIVEAGKKALTEAQIDP
                     AEIQAGAVGNFNAGQFTKQLQLGAFVPEIDPKLRGIPTMHTEAACASGALAVLLGAEW
                     IMGGFYDTVLVIGAEQQKTMSAVDGSDVLGAAADYHNEKPEYGDFMFPKLFGRIAQIY
                     IDKYGASEKDLAQVAWKNYAHARLNPMAQMRDADLSLDCASQVSEENPSVAPPLKVSD
                     CSQITDGAASVVLVSGKYLDRLGRDKSKMPRLLGFGHTTDYLPLDKKDVPTFSTARKA
                     AEKAFGMANLTPRDMQGAELHDCFSITEIVVYEILGLAEPGKGTELVKSGATALPQIR
                     NEKMRGKMAIEIPVNTGGGLIADGHPVGATGVRQVFDAYQQLSGKAGARQIENVKRFL
                     TFNMGGSLTTSVAMVWGRE"
     gene            complement(5921..6433)
                     /locus_tag="VFO30_07605"
     CDS             complement(5921..6433)
                     /locus_tag="VFO30_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419190.1"
                     /translation="MSQTDWEAISAVSQMVGSVAVVFSVLYLAVQVHRSTRIAKVTAQ
                     DMAATSLRDVTKPFAENAELARIWRVGLESLPSLSEEEQARFFHSTYQFLKAFETIHF
                     HHVHGLMDEQIWQGWCGLLEHYVAAPGIDHYWRLRHDLFSKRFQQFVQTLERPAERRT
                     VANLPGQERP"
     gene            complement(6452..7618)
                     /locus_tag="VFO30_07610"
     CDS             complement(6452..7618)
                     /locus_tag="VFO30_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9419191.1"
                     /translation="MTSLDYYAIEELLTDDEHAARDRTRRFVEEEVMQEIVPCHRAAK
                     FPEHLMVRMGALRLFAPYLKEHGCAGLSYTAYGLIMQELERADSGLRSVASVQGALVI
                     YAIHCFGTSDQQARWLQGLVSGERVGCFALTEHGHGSDPAGMETRATRNGDHYVLNGS
                     KCWIGNASIADVKVVWAKDDDGTVGGFIVEKNTPGFRAQDIEGKFSLRMSRTSECWFE
                     NCRIPAENRLPKASGLRAPLSCLSQARAGVAWGVIGAAIDCYETALAYAKAREQFGRP
                     IAGFQLVQEKLVWMVQEITKAQLLALRLTRLMEAGKAHPGQISLAKRNNTWMALECAR
                     QARDILGAVGITDRYSVIRHLMNLESVSTYEGTHDIHTLAVGRDITGLNAFGGS"
     gene            7683..9233
                     /locus_tag="VFO30_07615"
     CDS             7683..9233
                     /locus_tag="VFO30_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9419192.1"
                     /translation="MARLHEYQGKAILAAHGFRIPRGRAVSTADEAIALAKELVADKK
                     TAEVVIKIQAWTTGRASIGGVAFAKKPVDVRGHAARMLAMKVGQFPVAAVLVEEKIDI
                     EREFFLSFAIDDAARAPVIIFAGGGGTGIEERAAATRRIPCDVNRGPMDSAVDEAVGS
                     CGLSPAQAEQLAESIQKLFTAARSVEARSLEINPLVLTKDGQFVAADCRITIDDYAVA
                     RHPELNIEIAREFDHPPTPLERVAYAVEQSDHRGTFYFAQLATSAAKGSKGLVGFHGA
                     GGGGSMMSMDAIVNAGFTIANFTDTSGNPSASKVYRASRIILAQPDLVGYFGSGSGVA
                     SQEQYWSAYGLAKAFWELDLDIPAVIRLGGNTEDRAVDILHRTSKLLRAPVEGYRKTD
                     APAKIAARFAELVASAVRTKWKPRAPRIPKFVRNKSATTLPVKSGRVWIDTARWPDIR
                     RAVETHSGGLIVDHKGAPASSLPNEEFATKDSELLACDVECRLAGVDGFYLELDIPGL
                     NELIGGAR"
     gene            9234..>9649
                     /locus_tag="VFO30_07620"
     CDS             9234..>9649
                     /locus_tag="VFO30_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9419193.1"
                     /translation="MAILIDEKKRVLVQGITGREGRARARLMREYGTNVVAGVTPGKG
                     GQSVMGVPVFNTPQDAMNTLGKIDISVLFVPAAGVKDAAISAIEAGIKLTVLVPDRVP
                     IWDAMEIAAAANTNGAIFLGPNTLGVLSPGKAVVGM"
BASE COUNT         1991 a         2899 c         2709 g         2050 t
ORIGIN      
        1 gttgcgcgaa gcgttctcga tttcttcaac tccgtcgcag gaaagaaaat cctcaggcga
       61 atggcggagc tcgaaattga accaaagagt gaaaaactat cggccaagaa ggccgcgacc
      121 ttgcccttgg ccggcaagac atttgtcctc accggcacat tgccatcaat gacgcgtgaa
      181 gaagcgactg aaaagatcga gtcgttgggc ggtcacgtcg ccggcagcgt cagccggaaa
      241 accgattacg ttctcgccgg cgcggaaccc ggcagcaaaa tcgacaaagc aaaagaactc
      301 ggtgttcgtg tcatcgacga agccgagttt cgcaacgtgc tcggcgctta gcggatcggt
      361 accgctcagg ggcggcgttc cacctgcgcg tcgagtttga gcgggattgc ctggttcatg
      421 ctcggcgcca cgatcagcgg cggccgttga ctggctggat gcaggtcggc ctgcacgttg
      481 aaggtcagtt tatcgttgcc gcgcctgacc gtgatcggca cggtctcgcc ggccgtgatg
      541 aaaaacgacg catccagcac atcttcgggt tcgcgcactt ttttgttgcc cacttgcaaa
      601 agaatgtcgc ccgcctgaat gccggacttc gctgcgggcg tcccgttcat gatcttggtc
      661 atctccgcgc gcgagccttc gaccggcgtc tccgcttcag aaacattgat cccaacccag
      721 ccgtgccgta cctcgccaaa ccgcatgtaa tcgccgcgta tcttttcggc cgcgtcaatc
      781 ggcagcgcgt aacaggcaga attgttttcg agactgctta cgataatgcc aatgacttcg
      841 cctttgaggt ttaatagcgg cgcaccggct tcgccgcgtt gggttggtaa gttgacgcgc
      901 aaatgagtcg tcgaaaaata gcggccgaga tatttgcgat cgaagccggc gatcattcca
      961 aagctcggcg tctccggcag atcgagagga tagccgatac tcacgaccgg agtagcgatc
     1021 tcgagctggt cggacctccc aatgggcagg gccggcgtcg ccagatcaac ttttaagagc
     1081 gcgattccgc tgcggatatc ggccaaaagc tgccgcgccg gatatttttg cccgtcaaac
     1141 tcgatctcga aatcgccggc ctcacctccc accgtgtagg aagtgtaaag cgcgccggtc
     1201 ggatcgacaa agaagccggt accggcaatc tcgctgtgct cgtcaacgcc gtggatcttg
     1261 accaccgcgc gcgcggcgcc ggcgaaaatt cctttcactt cacgactgat cgactccggc
     1321 gctttctcct gcgcgagcag cagctgcgag ccggcgcaca aagtggcgac aaaaatcgcc
     1381 gtattcagcc acctgaattg agcgcgcaaa acagagcgcg acgcggagct agaagcggac
     1441 cgtggtctca taactcacag ggacgctgtc gagcacgtaa cgaggcggcg tcccgcgatc
     1501 gtgggagtga gtttgagcac gcaatttccg aagcggtttc gcgctaaaga gccgctccgc
     1561 gacgggcgaa gcaagcgtcc attcggcacc gagatttgcc ggtgcattcc aagatgtgtc
     1621 cgcacgcgct gcgaccgaag caggcggcga tggttggtac atttttagcg atatgaccgc
     1681 agcacaaacg agcaccgccg cagcagcggt tgcatacgag ccggtgctgc caaacacatt
     1741 gagacgcagg aagaaatcgt gcgtccggtg gaggcaaatg cgccagagcg gttcgcacag
     1801 caacgcgtcg cgctggcggc gatgaaactc gtgcaggaaa tcttcgaagt aaccgggcgg
     1861 cggttgttcg tagcgcttca gccgcagcag cgtcgcgatt tccttgtcat caaactcctc
     1921 catagcgccc attaagtgac tggatttttg cggaactcgt cgaggtaact ttgcaactgc
     1981 cgattcgcgt agaagagccg ggagcgtacc gtgccttcgg agactttcaa gattttactg
     2041 atctccgcgt ggggcatgcc ttgaatatgg aacatggtga ccacagctct gtgttcatca
     2101 gacaatttca tcatcgcctc gttcaatctt ctctgcagct cggacaagtc ggcttcgcgc
     2161 accggactgc tcttcgcggt cagctgcagc agttgcttgt cgctctgcac gctcgagtcc
     2221 acgtcgtcca ggctcatgtg aaaacggcgg ccgcgttttt tgaggaagtt caacgtcatg
     2281 ttcacggcga tggagtggat ccaggtataa aatgacgact tgccgcggaa gccgcgaatc
     2341 gccttgtagg ccttggcgaa gacgtcctgc aacaggtcgt tcgtgtcctc gtgattggcg
     2401 gtcatgttgt agaccagccc gtaaagtcgc ggcgaatatt tgacgacgag ttgatcgaac
     2461 gcgctcgcgt caccggcctg tgtccgcgcc accaactcct gatcttcgtc cgatttcggg
     2521 cgatccatcg cgttgccgct agacttagca cgcgcgtcta agcccgacga caacaaaccg
     2581 ggaaccgcga ccgatggatt catcttcttc aactcgcgcg attagcgccg gtcccgaatc
     2641 gacatcaacc agagcaggtt gccgagcgcg gcgatgaacg ccgcgacata cgtgagggca
     2701 gcggcgttca gcacgttgtt cacgccggcg acttcctgcc ccgactgtac aatacccatt
     2761 tcgcgcagaa tgattttcgc gcgccgagat gcatcgaatt ccaccggcaa cgtgatcaac
     2821 tggaacgcga gcaggatcat atagcagtaa atcccaagcg tgatcagacc tgtgaaccca
     2881 aaaaacaagc cgccaaaaat gacaaacggc agcatttgcg acgcaaccat tgtcaccggc
     2941 acaatggcca tgcgcgcctt cagcggcgcg taagctttcg cgtgctggat ggcgtgcccg
     3001 gtctcatgcg cagcgatgcc gagcgccgcg acagacggcg tgcgataaac attgctcgag
     3061 aggcacagtt tctttcgtgt cggatcgtaa tgatcgccga ggaaatcgtt cgtttccaca
     3121 acatcgacat catcaatccg cgcggcctgc aaaatctccc gcgcgcactc cgcgccggtg
     3181 atgttcgagg tggcgcgcac cttgccccag cggctgaacg cgctgctcac gcgaatctgc
     3241 gcccacagcc cgatcacgag cggaatcccg atgtaaatca gccagtgacc gccaaacccg
     3301 aagccgtaag gatagaacat tcctgtctta agtgtgtctg cttaacttag acgcgcaacc
     3361 gctcgctgcg ttcagatttt gcggcagtct ccactcggtc gacaaaaaca ccctcacctc
     3421 aatcctctcc ctgagggaga ggagaacgcg acagcgccag gtgagggtca cctcctaact
     3481 gcatcggtaa ctcaaatttc gagttgcttg gggaaacgcg agagaatttt gcagccagtt
     3541 tttgtcacca ccacgacgtc ctcatgccgc acgccgccga gaccgggata atacagaccc
     3601 ggctcgacag tgagcacttg tcgatctttc aatttcactt tttgcaagcg cggatactcg
     3661 tgaatatcga gcccgaggcc gtggccggtg ccgtggaaaa agccgacgtt ctttccatcg
     3721 cggagttccg ttggaaaact gcgatcggcg aaaagttttt cgattgcttt gtggattgtc
     3781 attccctcca cgcccgcttt aatgcgcttg agcgcgagct cctgtccctc cttcacggtg
     3841 tgccaaagtt tgcgctgcgc gtcggtcgca cgcccgcgca cgacggttcg tgtcatatcg
     3901 ccccagtaac cgctccttgg gtcgcgcggg aaaatgtcca aaatgattaa ctggttcgcg
     3961 cgcagcggac cggagccgcg ttcatgcgga tcgcaggcct gatcgccccc ggcgacgatc
     4021 gtgtttgtcg gtgtgccgcc agcccgcaac agtgcggtat caatttcggc gcgcagtatc
     4081 tcggaggtca gcaccttgcc cgaccatttt agcttcttgt cacgcgcgat tgtcgatttt
     4141 cgcaatacct cgattgcgcg gcgcattccg gcttcggtga tgcgcaacgc tcttccgatc
     4201 aacttgagct cctcgtccgt cttcgcttcg cgctccggcc aaaacaaacc attggttggg
     4261 gcaagatcga catgttcacg ggcgagttcg cgcgcgtagc cgagcggaaa attcatcggc
     4321 acggtcgcgc gtttcacgcg gcgcttgcgc agaaaatgtg cgagcacttt ttcgaatggc
     4381 ggcgcctgct tcgattttcc ctgcacttcg cgctcgagtc ggttgtagga aacgaactca
     4441 tccaccttcg ccgtccgacg gccgcgatcc atttccagat cggtcagcac gatcgtgcgc
     4501 ttgccgttct gctggagaaa aatgaacgta tcgcctgccc aaaatcgggt cgcatagagc
     4561 atatccgcgt cctgatcgct cgcggcgaca atcaagcgtg tgccattcgg tttcgtttta
     4621 ctcattcgcg gccccaaacc atcgccacac tggtcgtcag gctccccccc atgttgaatg
     4681 tgagaaagcg tttcacgttt tcgatctgcc gcgcgccggc ttttccgctg agttgctgat
     4741 aggcatcaaa tacctggcgc acaccggtcg cgcccaccgg atgaccatcg gcgatgaggc
     4801 cgccgccggt gttaaccgga atttcgatgg ccatcttgcc gcgcatcttt tcattccgaa
     4861 tctgcggcag ggcggtcgca ccgctcttca ccagctcagt gcctttacca ggttccgcaa
     4921 gaccgaggat ttcatagacg acgatttcgg tgatcgagaa acaatcgtgc agttccgcgc
     4981 cttgcatatc gcgcggcgtc aggttcgcca tgccgaaagc tttctccgcg gctttgcgtg
     5041 cggtcgaaaa cgtcggcacg tcttttttgt ccagcggcag gtaatccgtg gtgtgaccga
     5101 agccgagcag ccgtggcatt ttgcttttat cgcggccgag ccggtcgaga tattttcctg
     5161 agacgagcac gaccgacgcg gccccgtcgg taatttgcga acagtctgag actttcagcg
     5221 gcggcgcgac gctcggattt tcctccgaca cttgcgaggc acaatcgagc gacagatccg
     5281 catcacgcat ctgcgccatc ggattcaggc gcgcgtgcgc gtaatttttc cacgcgacct
     5341 gcgccaggtc cttttcgctc gcgccatatt tgtcgatgta aatctgcgcg atccgcccga
     5401 acaacttcgg gaacataaag tcgccgtact ccggtttctc gttgtgataa tcggccgctg
     5461 cgccgaggac gtcggaaccg tcaaccgccg acattgtctt ctgctgctcg gccccgatca
     5521 ctaacacggt gtcgtagaac ccgcccatga tccattccgc accaagcaac actgccagcg
     5581 cgccgctcgc gcacgccgct tccgtgtgca tcgtcggaat gccgcgcaac ttcgggtcga
     5641 tctccggcac aaacgcgccg agctgcaatt gtttcgtgaa ctgcccggcg ttgaaattgc
     5701 caactgcacc cgcctggatc tccgccggat cgatctgcgc ctctgtgagc gccttcttgc
     5761 cggcttcgac aatcaggtgc cgaatcgttt tcccttcctt cttcaggttg cgcttaaaat
     5821 ccgtccgcgc accgcccagg atgtaaactt cgttcatgca ccgataaatc tagctaatca
     5881 tcgcaccgtg tcatgcacac gtgggcgtga atctgcgcgg tcacggccgt tcctgccctg
     5941 gcaggttcgc cacggtccgc cgctccgccg gccgttcgag cgtctggaca aattgctgaa
     6001 agcgcttcga gaagaggtcg tggcgcagtc tccaataatg gtcgatgccc ggcgccgcga
     6061 cgtagtgctc aaggagaccg caccagccct gccagatctg ctcatccatc aacccgtgga
     6121 catggtgaaa atggatggtc tcgaacgctt ttagaaattg gtaagtcgag tggaaaaagc
     6181 gcgcctgctc ttcctccgac aggctcggca gactttccag accaactcgc cagatccggg
     6241 cgagttcggc gttctcggca aatggtttcg ttacatcgcg cagcgacgtc gccgccatgt
     6301 cctgtgccgt cacctttgcg atgcgcgtgc tgcgatgcac ttgcacggca agataaagaa
     6361 ccgaaaacac caccgcaacg gagccgacca tttgcgaaac cgcgctgatc gcttcccagt
     6421 ccgtttggct cattctcgcg cagtttacca attaggagcc gccgaaagca tttagtccgg
     6481 taatgtcacg accgacggcg agtgtgtgga tgtcgtgcgt accctcgtaa gtggaaacgc
     6541 tttccaggtt cataagatgt cggatcacag aatagcggtc tgtgattcct accgcaccca
     6601 ggatgtcgcg tgcctgtcgc gcgcattcca gcgccatcca cgtgttgttc cgtttcgcca
     6661 gcgatatttg cccgggatgt gccttgcctg cttccatcag tcgggtaagg cgcaacgcca
     6721 ataattgtgc cttggtaatc tcttgcacca tccagacgag tttttcctgc actaactgga
     6781 agccggcgat cgggcggccg aattgttcgc gcgctttagc gtaggcaagc gccgtttcgt
     6841 aacaatcgat agcggcgcca atcacacccc acgcgacgcc ggcgcgcgcc tgtgaaagac
     6901 atgataacgg cgcgcgcaat ccggaggcct taggcaagcg attctccgct ggtatccggc
     6961 agttttcgaa ccagcattcc gatgtgcgtg acatgcgaag gctgaacttt ccctcgatat
     7021 cctgcgcccg aaatccggga gtatttttct cgacgatgaa tccgccgaca gttccgtcat
     7081 cgtctttcgc ccaaacaacc ttcacatccg cgatcgacgc gttaccaatc caacatttgc
     7141 ttccgttcaa gacataatga tcgccgttgc gtgtggcgcg cgtttccata ccggcaggat
     7201 ccgatccgtg gccatgttcg gtcagggcga aacaacctac gcgttcaccc gagaccagtc
     7261 cctgcaacca ccgcgcctgc tggtcggacg tgccgaagca atggatcgcg tagatcacga
     7321 gggcgccctg caccgacgcg actgagcgca aacctgagtc cgcccgttca agctcttgca
     7381 tgatcaaacc gtacgctgtg tagctcagtc ccgcacaacc gtgctcttta agatacgggg
     7441 caaaaagacg caacgcaccc atgcgcacca taagatgttc cggaaacttc gccgcacgat
     7501 ggcacggcac gatctcctgc attacttctt cctccacaaa acgccgcgtc cgatcgcgcg
     7561 ccgcgtgctc gtcgtcggta agaagctctt ctattgcgta ataatccagc gaggtcatgc
     7621 aatgcccggc ttttcaacag tgctatatct tttaggattc tgattcgcaa tagcaccgaa
     7681 cgatggcacg acttcacgaa tatcaaggca aagccattct cgctgcccat ggattcagaa
     7741 ttcctcgtgg ccgcgctgtt tcaaccgccg acgaagcaat cgcgctcgcc aaggaactcg
     7801 tcgcggacaa gaaaaccgcc gaagtcgtta tcaagattca agcttggacg acaggacgcg
     7861 ccagtattgg cggtgtggcc ttcgccaaga aaccggtcga tgttcgcggc cacgcggcgc
     7921 gcatgctggc gatgaaagtc ggacagtttc ccgtcgcggc cgtgttggtg gaagagaaga
     7981 tcgacatcga gcgtgaattt tttctctcgt tcgcgattga tgacgccgcg cgcgccccag
     8041 tgattatctt cgccggcggc ggcggcaccg gaatcgagga acgcgccgcg gcaacgcggc
     8101 gcatcccgtg cgacgtgaat cgcggtccga tggactcggc agttgatgaa gcggtcggct
     8161 cttgcggact ttcgcccgca caagcggaac aactggccga gtcgattcaa aaattattta
     8221 ccgccgcgcg gagcgttgaa gcccgttcgc tcgaaatcaa tccgttggtc ctgaccaaag
     8281 acggtcagtt cgtcgccgcc gattgccgta tcacgattga tgattacgcc gtggcgcgac
     8341 atccggagtt gaatatcgaa atcgcgcgcg aattcgatca tccgcccacg cctcttgaac
     8401 gcgtggccta cgcggtcgag caaagcgatc atcgcggcac attttatttc gcgcagctag
     8461 cgacgagtgc cgcaaaaggc tccaaaggtc tggttggttt tcacggcgcc ggcggcggcg
     8521 gatcgatgat gtcgatggac gcgatcgtga atgccggctt caccatcgcc aatttcaccg
     8581 acaccagcgg taatccctcc gcgtccaagg tgtatcgcgc cagccgaatc attttggccc
     8641 agcctgacct cgttggatac ttcggttccg gctccggcgt cgcgagccag gaacaatatt
     8701 ggtccgccta cggattggcc aaagcatttt gggagcttga tctcgatatt cccgcggtca
     8761 ttcgtttggg tggcaatacg gaggatcgcg ccgtcgacat tttgcaccga acgtccaagc
     8821 tgcttcgtgc gccggtcgag ggttatcgca aaacggacgc gccggccaaa atcgccgcgc
     8881 gctttgcgga acttgtcgca agcgcggtcc gcaccaaatg gaaaccgcgc gcgccacgta
     8941 ttccgaagtt cgtaagaaac aaatcggcca ccacacttcc ggtcaaaagt ggtcgtgtct
     9001 ggatcgatac cgcaaggtgg ccggacattc gtcgcgctgt cgagacgcat tccggcggat
     9061 tgattgttga tcataaaggt gcacccgcgt catcgctgcc taatgaggag ttcgcgacca
     9121 aagattccga gttgctcgcc tgcgacgtcg agtgtcgact cgccggagtc gatggcttct
     9181 atcttgaact cgatattccc ggactgaacg aattgattgg aggcgcccgt taaatggcaa
     9241 ttttgattga cgagaaaaag cgcgtgctgg tgcaagggat caccggccgg gaaggccggg
     9301 cccgcgcccg tctgatgcgc gagtacggaa cgaacgtggt ggcgggtgtg acgccgggaa
     9361 aaggcgggca aagcgtgatg ggcgttcctg tgttcaacac gccgcaggat gcgatgaaca
     9421 ctttgggcaa gatcgacatc tcggttcttt ttgttccggc ggctggcgtg aaagacgccg
     9481 ccatttcggc gattgaagcc gggatcaaac tgaccgtgct agtgcccgat cgtgtgccga
     9541 tatgggacgc gatggaaatc gcggccgcgg ccaacaccaa tggcgcaatc tttcttgggc
     9601 ccaatacgct cggtgtttta agtccgggta aggcggtagt tgggatgat
//
LOCUS       DASTDG010000228         6628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1292904, whole genome shotgun sequence.
ACCESSION   DASTDG010000228 DASTDG010000000
VERSION     DASTDG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292904"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFO30_07625"
     CDS             <1..529
                     /locus_tag="VFO30_07625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9419194.1"
                     /translation="VNPLHGTAAECRDFYNTHQERFLQPTRCRASHLFLAAPPETPPE
                     IVDLKQKIIASLWERVRKGEDFSELVAANSEDEATKARGGDLGFFSESRMPPDFFEAV
                     QKLRVGEISRPVRTRLGFHIIQVTDVKLPRQMSFEEAQPEIALAFENANLASAMRSIS
                     RQLVERAEFVRAALR"
     gene            609..1835
                     /locus_tag="VFO30_07630"
     CDS             609..1835
                     /locus_tag="VFO30_07630"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9419195.1"
                     /translation="MPTSRQTRKNVGIIGLGIIGGRVAENLRKKGFRVFVWNRTPRPV
                     PNFVGAPAELAEICDYIQIFVSDDHALLKTVEELTPALAPRHVIMAHSTVAPASMLRA
                     ADIIERRGARFVEAPFTGSRIGAEKGELVYYVGGDEVALREARPILEASSKEIMVIGD
                     IGQATAVKLATNIVTAASVQAMAEALALVQSVGLRVEKFVEALRGNASNSATLAMKLP
                     KMIEGDFEPHFSSKHMLKDMEIATRLGSTHNLELSVTTATRDRLLEQVQQGHQDEDYS
                     AVICKYSSARESTPAPEIALRESEFPATAAAAAEASLDLPQPQPEKPEDFVPMMPEIE
                     KEEMAEPAIGTTPQPAVVPPSAPDELASAAPPESQAATPPGEQAKEDTEGDEDFLARP
                     FGPATDAEKPAASEGK"
     gene            1838..2743
                     /locus_tag="VFO30_07635"
     CDS             1838..2743
                     /locus_tag="VFO30_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9419196.1"
                     /translation="MPDRATGKFFDFSSRAKLRVSGRDRVRFLNGQVTNDIRKASESR
                     AIEACILTAKGKMDAHAFVHAAGDCFLLDTEPELRESLRPRLERYIIADDVQVEDVTD
                     KLSILHLLNADAPQVSRPCTVVSATRFAEPGWDIWTDAADRDEIFRELASLFEFCDAD
                     CAEMLRIWQGIPRWGRELSPAIIPVEANLDVRCIDYEKGCYIGQETISRMKMSGQQNK
                     RLCGLIAPPESGLSPGMHLLPETDGKEVGWLTSVARSTSPARQIALGYVKRGFNAAGS
                     RLRATVGDERAAAVDVEIVDLPFRD"
     gene            2839..3483
                     /locus_tag="VFO30_07640"
     CDS             2839..3483
                     /locus_tag="VFO30_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9419197.1"
                     /translation="MNQRLLAEIGRTQRLEILNSLKRSKGMSVNELVAKLNMSYMGIK
                     QHCTTLQRDGYLDTWRRPQKMGRPEMVYRLTPRTHDLFPADSNRLTLELLNSVGEIYG
                     PNAPEKLLYNVYERKTAELKKKAKGDGVAERAKWLARARDNEGYMSQFLTDSKDGGPQ
                     ILECHSPVMNLLNAYPIVARLEQDMFEAVLGTLVRREEKRDSGQYECAFYFALD"
     gene            3560..5530
                     /locus_tag="VFO30_07645"
     CDS             3560..5530
                     /locus_tag="VFO30_07645"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET9419198.1"
                     /translation="MVLAFPTAVIENLSPLVDGGRYPVKRVAGDDLVVEADIFKDGHD
                     VVSAVLKWRLVGEERWHETPMVHVDNDRWRGACTLYENATYEYTVEAWADTFRGWQRE
                     FRAKFEAGVSALTAEALEGAALLDAAAQRAHVSADATRLHELSECIRTADDAQINEIA
                     HSGELDVLMSTYPDRSAATQYAPAPRVVVDRAAARVGAWYEFFPRSAEGRADRASTFR
                     DCLPRIDDARAMGFNVIYFPPIHPIGRTNRKGRNNALVAQPGDPGVPWAIGNEAGGHK
                     AVEPSLGTLEDFDWLQNEVRKRGMEIALDLAINCSPDHPYVREHPEWFYKRPDGSVKY
                     AENPPKKYEDIYPLNFRCENWLELWAEMLSIVLFWAERGVRIFRVDNPHTKPVAFWEY
                     LIANVREPYPDVVFLSEAFTKPKMMKALAKAGFSQSYTYFTWRNSKQELTDYLTELTE
                     TDMREYFRGNLWPNTPDILPFILQDGGRPAFIIRVVLAATLSSTYGIYSGYELCENEA
                     LPGREEYLDCEKYQLKERDWNAPGNIKDWITRLNQIRAENRALELYDNLRFYHADNPA
                     ILFYGKMTEARDNIILVVVNLDPHRKQHSFVDVPIDQFGAMEGDTYQVHDLLTDARYT
                     WHGRRNYVELDPDIQPAHIFRVRRWLGGDRFA"
     gene            5618..6403
                     /locus_tag="VFO30_07650"
     CDS             5618..6403
                     /locus_tag="VFO30_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9419199.1"
                     /translation="MKIAFSLLAALAGATPVLAQNVVTSPTPPLVPDLPRSKSVLDAL
                     IAGGPVMYLLFALSILFVMLVITYLMTIRRGAVVSSGFMATADALLRKRDYLGLLAVS
                     NRHGEAIARVVQKVLDFTTKNPNSDFQQVREIAETEGGRVAASLNNRVTYLADIGMIA
                     PLLGLLGTVIGIIRSFGALSGAELGAQRYIALSQGISQALINTCAGLAIGIPAMIFYA
                     YFRGKSQKLISDLESASTHVLALLSLHHTRRDERAPVLIEDEL"
BASE COUNT         1389 a         1935 c         1930 g         1374 t
ORIGIN      
        1 ggtaaacccg cttcatggca cggccgctga atgccgggat ttctacaaca cgcaccaaga
       61 gcgtttcctc cagcccacac gatgtcgggc gagtcacctc tttttggctg cgccgccgga
      121 aacgccgcct gaaattgtgg atcttaaaca aaaaataatt gcttctctgt gggagcgcgt
      181 ccgcaagggt gaagacttct ccgagctggt cgcggctaac tctgaagacg aggcgacgaa
      241 ggcgcgtggc ggtgatcttg gatttttttc cgagtcacgg atgcccccgg atttctttga
      301 ggcggtgcaa aaattacgcg ttggtgagat tagccgcccg gttcggacgc gcttgggatt
      361 tcacatcatt caggtaactg acgtgaagct gccccgacag atgagcttcg aggaagcgca
      421 gccggagatc gctctggcgt ttgagaacgc gaacctcgcc agcgcgatgc gcagcataag
      481 caggcagctg gtggaacgcg ccgaatttgt gcgggccgcg ctccggtaac tttaagccac
      541 ctctccaccg ctgctgcggg ttgacttctc actgatccgg cgcgaatagc cggactgcgc
      601 ttgaacgaat gcctacctct cgacagacgc gtaagaatgt gggtatcatc gggctcggta
      661 ttatcggcgg ccgggtagcg gagaacctgc gcaaaaaagg ttttcgcgtt tttgtctgga
      721 accgcacgcc acgacccgtg ccaaacttcg tcggcgcgcc ggccgagtta gccgaaatct
      781 gtgactacat ccagatcttc gtctctgacg atcacgcgct gctcaagaca gttgaggagc
      841 tcacgccggc gctcgcgccg cggcacgtca tcatggcaca ttccacagtc gcgccggcat
      901 cgatgctgcg agctgccgat atcatagaac gacgtggcgc gcgctttgtc gaagcgccgt
      961 tcaccggaag ccgcatcggg gcggaaaaag gcgagcttgt ctactacgtc ggcggagacg
     1021 aagtggcctt gcgtgaggcg cgtccgattt tggaggcaag cagcaaagag atcatggtga
     1081 tcggagacat tgggcaagcg acagcagtga aattagcgac caacatcgtg acagcagcga
     1141 gcgtgcaggc catggccgaa gctcttgcgc ttgttcaaag cgttggcttg cgcgttgaga
     1201 aattcgtcga agcgttgcgt ggcaatgcca gcaattcggc caccttagcg atgaaattgc
     1261 cgaagatgat cgaaggcgat ttcgaaccgc atttctcgtc caaacacatg ctcaaggaca
     1321 tggaaatcgc gacgcggctc ggttcaactc acaacctcga gcttagcgtc acaaccgcca
     1381 cgcgtgatcg gcttctcgag caggtgcagc aaggacatca ggatgaggat tactccgctg
     1441 tgatctgcaa atattcgtcc gctcgagagt cgacgccggc cccggaaatc gctctgcggg
     1501 agtccgagtt tccggcgacc gcggccgcgg cggcagaagc gtcgctggac cttccacaac
     1561 cccagcccga aaagccggaa gacttcgttc ctatgatgcc ggaaattgaa aaggaagaaa
     1621 tggccgagcc tgcaatcgga acaacgccgc aacctgcagt tgtgccgccg tcggccccgg
     1681 acgaactcgc gagtgcagcg ccgccagaat cgcaggccgc tacgccaccg ggcgagcaag
     1741 ccaaagaaga cacggaaggc gatgaagatt ttctggcgcg tccctttggc cccgcgacgg
     1801 acgccgaaaa acccgccgct tcggagggaa aataactgtg cccgatcggg cgacaggaaa
     1861 gtttttcgac ttctcctcgc gcgcgaagct gcgcgtttcc gggcgggatc gagtgcgttt
     1921 tctcaacggg caggtcacaa acgatattcg caaagcgagc gagtcgcgcg cgatcgaggc
     1981 ttgcattctg acggcaaagg gcaaaatgga tgcccacgcc tttgttcacg ctgcaggcga
     2041 ttgttttctc ctcgatactg aacctgagtt gcgcgaatcg cttcggccgc gactcgagcg
     2101 ctacatcatc gccgatgacg tacaggtgga agatgtcacc gacaaattgt cgattttgca
     2161 cctcttgaac gctgacgcgc cgcaggtgag caggccgtgc acagtcgttt cggctacgcg
     2221 cttcgcggag cccggctggg acatttggac tgacgcggcc gaccgcgatg aaatattccg
     2281 tgagctggcc tcgctgttcg agttctgcga cgccgactgc gccgaaatgc ttcggatttg
     2341 gcagggcata ccgcgatggg gtcgcgagtt gagtccggcg atcatcccgg tcgaagcaaa
     2401 cctcgacgtg cgctgcatcg attacgagaa gggctgttac atcgggcagg agacgatctc
     2461 gcggatgaaa atgtccgggc agcaaaacaa acgtttgtgt ggtttgatcg cacctcctga
     2521 gtcgggtttg tcaccgggaa tgcacttgct gccggagacg gacggcaagg aagttggctg
     2581 gctcacgagc gtcgctcgca gtacgtcccc ggccagacaa attgcgctgg gctacgtcaa
     2641 gcgcggcttc aacgcggctg gctcgcgact tcgagccacc gtcggcgacg agcgtgccgc
     2701 agcggtcgac gtagagattg tcgatcttcc gtttcgagac tagccgcgtg gcccgcgggt
     2761 tacgaagctt gcgtaattat cttgcaatca atgggattgt gccgcataga atccgcgatg
     2821 tcatttcctc tcaccgctat gaatcaacgc ttacttgcgg aaatcggtcg cacccagcgc
     2881 ttggagattt tgaattctct gaagcgctcc aagggcatgt cagtcaatga gttggttgcc
     2941 aaactcaaca tgagctacat gggtatcaaa cagcattgca ccacccttca gcgcgatggc
     3001 tatctggata cttggcgaag gccgcagaag atgggccgcc cggaaatggt ttatcgcctg
     3061 acgccgcgca cccacgatct gtttcccgcc gacagcaata gactgacgct cgagttgttg
     3121 aactcggtgg gcgaaatcta tggcccgaac gccccggaga aacttcttta caatgtctac
     3181 gagcgaaaaa ccgccgagct gaaaaagaaa gccaagggcg acggtgtggc cgaacgcgcg
     3241 aaatggctcg cgcgcgcccg cgacaacgaa ggttacatgt cacagtttct caccgattcg
     3301 aaagacggcg gaccgcaaat tctcgagtgt cattcgccgg tcatgaactt gctcaatgcc
     3361 tacccaatcg ttgcgcggct cgagcaggac atgttcgaag cggtcctcgg aacgctggtt
     3421 cgccgagaag agaagcgtga ttccggtcag tacgagtgcg cgttttattt cgcgctcgac
     3481 tgattcgttg tcgatcttcg cgcgcacagc tcgatttccg ctttcgtgcg cgcgctaatt
     3541 tcagttcact ggtggcccca tggttctggc ctttcccact gccgttatcg aaaacttgtc
     3601 cccgctcgtc gatggcggcc gttacccagt gaaacgcgtc gccggcgacg atctcgtggt
     3661 cgaagccgac atatttaagg acggccatga tgttgtctcg gcggttttga agtggcggct
     3721 ggttggcgag gaacgttggc acgaaacacc gatggtgcac gtcgacaatg accgctggcg
     3781 cggcgcgtgc acgctctacg aaaatgcgac ctacgagtac acggtggaag cttgggccga
     3841 caccttccgc ggttggcaac gcgaattcag agcgaagttc gaggccggcg tctcggcatt
     3901 gacggcagag gcgctcgagg gcgcggcctt gctcgatgca gccgcgcagc gcgcgcacgt
     3961 ttcggctgat gccacgcgtc tgcacgagct atctgaatgc atccgcaccg cggatgacgc
     4021 gcagatcaac gaaatcgcgc attctggtga gctcgacgtc ttgatgtcga cttacccaga
     4081 tcgttccgcc gcgacacaat atgcgcctgc gccgcgcgtg gtcgtcgatc gtgcagccgc
     4141 acgcgtcggc gcgtggtacg aattttttcc ccgctccgcc gaagggcggg ccgaccgcgc
     4201 atccacattt cgcgactgtc tgccgcgcat cgacgatgca cgggcgatgg gtttcaacgt
     4261 gatctacttt ccaccgattc acccgattgg tcggaccaac cgcaaaggtc gcaacaacgc
     4321 actcgttgcc cagccgggcg atcccggggt gccgtgggcg attggaaacg aagcgggcgg
     4381 ccacaaggcg gtcgagccgt cgctcggcac actggaagac ttcgattggc tacaaaacga
     4441 ggtgcgcaaa cgtgggatgg aaatcgcgct cgacttggcg atcaactgtt cgccggatca
     4501 tccctatgtg cgcgagcatc cagaatggtt ctacaaacgc ccggacggct ccgttaaata
     4561 cgcggagaat ccgccgaaga aatatgagga catctacccg ctgaatttcc ggtgcgagaa
     4621 ttggctggag ctttgggcgg agatgctcag catcgtgctg ttttgggccg agcgcggtgt
     4681 ccgcatcttt cgcgtggaca atccgcacac caaaccggtc gcgttttggg aatatctcat
     4741 cgcgaacgtg cgcgagcctt atccggacgt cgtttttctt tccgaagctt tcacgaaacc
     4801 aaaaatgatg aaggcgctcg ccaaggcagg gttcagccag agctacacgt atttcacatg
     4861 gcgcaattcg aaacaggaac tgaccgacta tctgaccgaa ctgacggaaa cggacatgcg
     4921 cgaatatttt cgcggcaatc tttggccgaa cacgcccgac attttgccgt ttattcttca
     4981 ggatggcggc cggccggcgt tcatcattcg cgtcgtgctc gcggcgacgc tctcaagcac
     5041 ctacggtatc tacagcggct acgagctgtg cgagaatgaa gcgttgcctg gtcgagagga
     5101 atatcttgat tgcgagaagt atcagctgaa agaacgcgac tggaatgcgc cgggcaacat
     5161 caaggactgg atcacccggt tgaatcaaat ccgcgccgaa aatcgcgcgc tcgagttgta
     5221 cgataacctg cgcttctatc acgccgataa cccggcaatt ttattctatg gcaagatgac
     5281 tgaggcgcga gacaacatca ttctggtcgt cgtcaatctt gatccgcatc gcaaacagca
     5341 ttcatttgtc gatgtaccga tcgatcagtt tggcgcgatg gaaggcgaca cctaccaggt
     5401 gcacgacctg ctcaccgatg cgcgttacac ctggcacggc cggcgcaatt acgttgagct
     5461 cgacccggac atccagcccg cccacatctt tcgcgtccgc cgctggttgg gcggcgatcg
     5521 gtttgcatag caaccccgca ctcttttgcg ggttaacagt ggcgacgcgc tatttgcatc
     5581 cgataacggc gcgtttattc taaccggaat tttcaggatg aagatcgcgt tctcgctgct
     5641 cgccgcgctg gcgggcgcaa cccccgtgct cgcgcaaaac gtcgtcacca gccccacgcc
     5701 gccgcttgtt cccgatctgc cgcgttccaa aagtgttctc gacgcgctca ttgccggcgg
     5761 gccggtcatg tacttgctct tcgcgttgtc gatcttattc gtcatgcttg tcatcaccta
     5821 cctgatgaca atccgccgcg gcgctgttgt ttcgagcggt ttcatggcga ccgccgatgc
     5881 gttgctgcgc aaacgcgact acctcggtct actggccgtc tcgaatcgcc acggcgaagc
     5941 tatcgcgcgc gttgtgcaaa aggtcctcga ttttacgacg aaaaacccga actccgattt
     6001 tcaacaggtg cgcgagatcg ccgagactga aggcggccgg gtcgcggcga gtctgaacaa
     6061 ccgcgtcact tacctcgccg acatcggcat gatcgcgccg ctgcttgggt tgctcggcac
     6121 agtcattgga atcatccggt cattcggcgc gttgagcggc gcggaactcg gggcgcagag
     6181 atacattgcg ctgtcgcaag gaatcagtca ggccctgatc aatacctgcg ccgggctcgc
     6241 catcggcatc ccggcgatga ttttttatgc ctatttccgc ggcaaatcgc agaagctgat
     6301 ttccgatttg gaatcagctt ccacacatgt gcttgccctg ctttctctcc accatacacg
     6361 acgcgatgag cgcgcgccgg tcttgatcga agacgaactg taatttgttc gccgtgcgac
     6421 cgcgtttgca agatcgacat cttttactgc gatgaatctc cgcggccgcg cgccgatcca
     6481 tcatcctggc atccagctcg cccctctggt tgatgttctg cttctcctac tcatcttctt
     6541 ccttctcact tggaatgcgg cccggaacga gaacgaattg gacgtcaaag tccccaaggc
     6601 atcagccgca aaagagaaaa gcgcgccc
//
LOCUS       DASTDG010000229         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_928315, whole genome shotgun sequence.
ACCESSION   DASTDG010000229 DASTDG010000000
VERSION     DASTDG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928315"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFO30_07655"
     CDS             <1..199
                     /locus_tag="VFO30_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9419200.1"
                     /translation="WYHFRHLVKPGITGWAQVNYPYGESETDAMEKLKYDLYYIRHYS
                     LKLDAMIVLRTVHTMLFGRGR"
     gene            287..706
                     /locus_tag="VFO30_07660"
     CDS             287..706
                     /locus_tag="VFO30_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9419201.1"
                     /translation="MNERQRVTTFEDLECWKECRKLRLFVVKEVVPVLPKDERYRLGD
                     QLSRAARSTTANIAEGYGRFHYLENAKFCSNSRGSCWEVLDHLITASDENLISSELLE
                     RGREQVGRAVRLLNGYMNYLKRTDRTARIAEPITENE"
     gene            723..2066
                     /locus_tag="VFO30_07665"
     CDS             723..2066
                     /locus_tag="VFO30_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419202.1"
                     /translation="MKLLGSASRWIFVAALAYAPWAYGGTTASSIATLNWLLVATLAL
                     WLVDLSIRRRVPKFPPVLIISIAALTGIGAWMAFNASAICDSEFHSFVALAQPAPRLP
                     GSIDYALSAAWMIRVGLLLGILLFVVDLARDDRWLLWLWSAIGVIAGSIALLGLLQKA
                     TGAGTIFWQPAPPYASRTFFATYYYHGNAGAYLNLVLPLTAGLVVRAFLTRGNSASRA
                     LWLTIFVLTLVAVAANTSRMAQLIALMLLLALAWQLGPRVARRLSRTEKNVALGGAAA
                     ILAALLAVAQASHLEQPIQRWEGISITQDARWLAATVAMRSIAQAGAFGFGPGTFRAV
                     FPWLNDSASVHAPGYWRFLHEDYLQVALEWGWLGSLPWAVVFFGGIFAAIRAFRAPNS
                     RKWAPRRRLLLPLVITALAGVALHGLVDFPLQISSIQLYVATYLGLCWGAKTWTS"
     gene            complement(2136..>3861)
                     /locus_tag="VFO30_07670"
     CDS             complement(2136..>3861)
                     /locus_tag="VFO30_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET9419203.1"
                     /translation="EAAVADYKAKTPDALQLGGGAAATGSQTGAGSSGGRGGLVEDKL
                     QELNSKLTVAKSERFRLEGELKQIETIGENVDALLAVPSIAAAPMVNDRRRDVAQLEA
                     NVATLAQRYKDRHPKMMAARAALNEGRDGLRRVVLAQPAVLRNTLEQARAAEGNLEAA
                     AKEQEKAALALNKAAIGYQELARQAETDRALYESVLRQIKETNLTKDVKTNAVSVVEH
                     SPLPRVPVSPSRSKALALGLLGGLAVGLFFVFGADAIDRSIKTVDEAEATIGLPVFAA
                     IPETTEQPRRSLFPSRHKRRTAGYRYRVIAETPEGPAAEAFRNLRAALSLLGPEAERK
                     VTLFTSAVPEEGKSFTSANYALALAQQGHRVLLIDADLRRPSMQKVFHLATNDNESAD
                     TPGIVDCLVGEVDLTTAARSISAEDIDAVKDHLATGGALLTATGGQLFVLASGHRAPN
                     PAEILSGPAFSQLIAEAARLFDRVVLDSAPILAVSDTLLMTKHVQTVCMVVRARKTPR
                     PAVRRAIALLVSSGIRPAGLVLNRLPRSHGVGYYYYYASPGYGKGEGSYSSTYADNGE
                     PRNLSRTA"
BASE COUNT          715 a         1084 c         1177 g          885 t
ORIGIN      
        1 gtggtaccat ttccggcacc tggttaagcc gggcatcacg ggctgggcgc aggtaaacta
       61 cccgtatggc gagagcgaga cggatgcgat ggagaaactc aagtacgatc tctattacat
      121 ccggcattac tcgctcaaac tcgatgccat gattgtgctt aggaccgtgc acacgatgct
      181 cttcggccgc ggccgttagc cgcgaccgca gagcatcggg ttactggtta atagttatcg
      241 gttataagtt ctcggctgtt ttccgcccgc gtgataatct cggcgaatga atgagcgtca
      301 acgcgttacg acttttgagg atttggagtg ctggaaagaa tgtcgaaaac tgcgcctatt
      361 cgtggtgaaa gaagttgtac cggttttgcc gaaggacgaa cggtatcgat tgggcgatca
      421 gttgtcgcgc gccgcgcggt caacgaccgc gaatattgct gaggggtacg ggcgttttca
      481 ttatctggaa aacgcgaagt tttgcagcaa ttcgcgcggc tcgtgttggg aggttctcga
      541 ccatttgatt acagcgtcgg acgagaatct aatctcgagc gaattacttg aacgcggaag
      601 agagcaagtg ggacgcgcgg tcagattgtt gaacggctac atgaattatt tgaaacggac
      661 tgacagaact gcccggattg cagagcccat aaccgaaaac gaataactga taactgccgc
      721 caatgaaatt gctgggctcc gcttcgcgct ggatttttgt cgccgcgctt gcctatgcgc
      781 cgtgggcgta tggtggcacg actgcgtcaa gtattgcgac actcaattgg ctactagttg
      841 cgacgcttgc gctttggctt gtcgatctaa gcattcgtcg tcgcgtaccg aagttcccgc
      901 cagtgcttat catttcgatc gcagcgctga ccggtattgg ggcgtggatg gcctttaatg
      961 cgtcggcaat atgcgattcg gagtttcaca gctttgtagc tttagcgcag ccggcgccaa
     1021 gactacccgg ttcaattgat tatgcgctga gcgcggcatg gatgatccga gtcggacttt
     1081 tgctaggtat cctcttgttt gttgtcgatc ttgcacgcga cgaccgatgg ctgctgtggc
     1141 tgtggtccgc gattggggtc atcgccgggt cgattgcgtt gcttggcttg ctgcaaaaag
     1201 caaccggtgc ggggacgatt ttctggcaac cggctccgcc ttatgcttca cgcacgtttt
     1261 tcgcgaccta ctactaccac ggcaacgccg gcgcctacct gaatctcgtg ttgccgttaa
     1321 cggctggctt agtcgtgcgc gcattcctta cgcgaggtaa ctcggcgagt cgcgcgcttt
     1381 ggctcaccat ttttgttctg acgttggttg cggtagccgc caacacctcg cgcatggcgc
     1441 aactgattgc ccttatgctg ctgctcgcgc tggcctggca actcgggccg cgcgtggctc
     1501 gccggttgtc gcgcacggaa aagaatgtcg cgctaggtgg agcagcagcg attctggccg
     1561 cgttgcttgc cgtcgcgcag gcgagtcatc tcgagcaacc gatccagcga tgggaaggaa
     1621 taagtattac acaggatgcg cggtggttgg ctgcgactgt ggcgatgcga tcaattgcgc
     1681 aggccggcgc gttcggtttc gggcccggca catttcgcgc agtgtttccg tggttgaacg
     1741 attcagcgag cgtgcacgcg ccagggtatt ggagattttt gcacgaggac tatctgcagg
     1801 tagcgctgga atggggctgg ctcggcagtc tgccgtgggc cgtcgtgttt ttcggcggga
     1861 ttttcgccgc gattcgagcc tttcgtgcgc ctaacagtcg caagtgggct ccgcgccgcc
     1921 gtcttctttt gccgttagtt attaccgcgc tggccggagt ggcgctgcat ggtttggtgg
     1981 atttcccgct ccagatttca tccatccagc tttacgtcgc gacctacctt ggtctgtgct
     2041 ggggcgctaa aacgtggaca tcttagcaag tgagtagttc atcagagctt gatgcttatc
     2101 gcgctggtcg gcggctacgc gacaactgat cacgcttagg ctgtgcggct aagattccgg
     2161 ggctcgccgt tgtccgcata ggtagaggaa taggagcctt cgcccttacc ataaccaggt
     2221 gaagcgtagt agtagtaata gccaacgcca tggctgcgcg gcaggcgatt caagaccaga
     2281 ccagcaggac gaataccgga tgagacgagc agcgcaatcg cacggcgcac cgccgggcgt
     2341 ggagttttcc gggcgcgcac gaccatgcag accgtctgca catgcttggt catcaaaagt
     2401 gtgtcgctga cggcaaggat tggggcgctg tcgagtacca cgcgatcgaa cagccgggcc
     2461 gcttcggcga tgagctggct aaaagccggg ccgcttagaa tctccgccgg attcggcgcg
     2521 cggtggccgc tggcgaggac gaataattgt ccaccggtgg cagtcagcag cgcgccgcca
     2581 gtggcgagat gatccttaac ggcatcaata tcttccgcgg aaatggagcg cgccgccgtc
     2641 gtcagatcga cttcgccaac cagacagtcg acaattccgg gcgtatcggc ggactcgttg
     2701 tcgttggtgg cgaggtgaaa caccttttgc atgctgggcc gacgaagatc ggcatcgatg
     2761 agcaggaccc gatggccctg ttgcgcaagc gccagcgcgt agtttgcgct ggtaaaactc
     2821 ttgccttcct ccggcacggc gctcgtgaac aaagtcactt tgcgttccgc ctccgggccg
     2881 agaagtgaca gggcggcgcg caaattgcgg aatgcttccg cggctgggcc ctcaggggtc
     2941 tccgcgatca ctcggtagcg atatccggcc gttcgccgtt tgtgccttga tggaaaaagg
     3001 gatcgccgcg gttgctctgt tgtttccggt attgccgcga aaaccggcaa accgatcgtc
     3061 gcttcagctt catcaaccgt cttgatcgag cgatcgatgg cgtcggcgcc aaagacaaag
     3121 aacagaccga cagcgaggcc gccaagcaga cccagcgcaa gcgctttgct cctgcttgga
     3181 ctcactggca cgcgcggcag cggcgagtgc tcaaccacgc tcacggcgtt ggtctttaca
     3241 tccttggtaa gatttgtctc cttgatctgg cgaagcacgc tctcatagag cgcgcggtct
     3301 gtctccgcct gacgcgccaa ttcctggtaa ccgatcgcgg ccttgttcaa cgcgagcgca
     3361 gccttttcct gttccttcgc cgccgcttcg agattacctt cggcggctcg agcttgctcg
     3421 agggtgttgc ggagcacggc tggctgcgca agcacaacgc gacgcaaccc gtctctgcct
     3481 tcattcaacg ccgcgcgcgc ggccatcatt tttggatgtc tgtctttgta acgctgcgcc
     3541 agagtcgcga cgttagcttc gagttgcgcc acatcgcggc ggcgatcatt aaccatgggt
     3601 gcagccgcga tactcggcac cgcaagcaac gcatcgacat tctcgccgat ggtttcgatt
     3661 tgtttgagct cgccctcgag gcgaaaacgc tccgacttgg cgaccgtcaa tttgctgttc
     3721 aattcctgca gcttgtcttc gaccaaaccg ccgcggccac cagagctgcc ggcgccggtc
     3781 tgactgccgg ttgcagccgc gccgcctccg agctggaggg cgtccggtgt tttcgctttg
     3841 taatcggcca ccgccgcctc g
//
LOCUS       DASTDG010000230         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_542749, whole genome shotgun sequence.
ACCESSION   DASTDG010000230 DASTDG010000000
VERSION     DASTDG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542749"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFO30_07675"
     CDS             <1..861
                     /locus_tag="VFO30_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET9419204.1"
                     /translation="ANTATGWQTLFNNTGDNNTADGAFALFSNTTGHDNTADGFGALS
                     SNTTGHRNVANGEFALYSNTTGAFNTANGVDTLYSNTTGNNNIGLGNSAGGALTTGNY
                     NIDIGNEGVAGESHRIRIGTQGTHKRTFIAGIYGVAAAQGVAVYVNSSGQLGTLTSSA
                     RFKQDIQAMDDASDALLSLRPVTFRYKKEIDPEGIPQFGLVAEDVAKVNPDLVARDAQ
                     GEVYTVRYEAVNAMLLNEFLKEHRQVQKQQKQIENLTAQLKDQAAQIRKVNDKVEMNK
                     PVPQMVLNNQ"
     gene            1084..2469
                     /gene="glmM"
                     /locus_tag="VFO30_07680"
     CDS             1084..2469
                     /gene="glmM"
                     /locus_tag="VFO30_07680"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9419205.1"
                     /translation="MTPAKKIFGTDGVRGVANVEPVTAETALKLGRAAAHVFTQLNPR
                     TIPEGTRPKIVLGKDTRLSGYMLENALVAGITSLGVDVLIIGPLPTPGVAYITRSLRA
                     DAGIVLSASHNPYEDNGIKFFRHDGYKLDDQVEEQIEQLVFTGKIDNVRPTAGKIGRA
                     ARIDDALGRYVEFAKASFPKKMSLEGMRVAIDVANGAAYKSTPCILRELGAEVAVAHN
                     EPNGMNINDACGSTHPEEIQRIVNISGADVGITHDGDADRVLLCDENGEIVDGDEILA
                     IAGLDLLNNGQLRDKTLVATVMSNFGLDETIAEHGGKVVRTKVGDRYVIEDMLARNLN
                     LGGEQSGHIIFRDFTTTGDGIISALQILRIMHATGQPLGKLRTCLKKYPQAQRNLEVK
                     AKPPIEDLPEVMKLRDEIERELGGKGRILLRYSGTEPKIRLLIEGREFDKIDKQANRI
                     ADAIQSAIGAN"
     gene            2470..3111
                     /gene="scpB"
                     /locus_tag="VFO30_07685"
     CDS             2470..3111
                     /gene="scpB"
                     /locus_tag="VFO30_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9419206.1"
                     /translation="MTLGRVVEALLFSAQKPLSTKEILDLLRRASGSDEFSPNEFAKV
                     READVAAALQEIKIEYIEQQRAFQLVEKVDGWQLATDPQYARWVRELFPAPRPARLSA
                     PALETLAIIAYRQPITRADVEAVRGVSIDGVLQTLMERGLVKIGGRADIPGRPLLYET
                     TQFFLDHFRLRHLDELPNVEELRKRYLPVAPRASVAAVDDRGPASATPAATNL"
     gene            3142..4191
                     /locus_tag="VFO30_07690"
     CDS             3142..4191
                     /locus_tag="VFO30_07690"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9419207.1"
                     /translation="MNGSSRSRFAQRSGYSILALAFVTSTVAFGEIKPSDCARAAKYS
                     DSRRGMSMLVMQNGRTVFEHYAHGGMPELRSPIFSGTKSFWGIAALIAVQRRLFQLDD
                     RVADTITEWKNDPGKSQMTIRELLNFTDGIEGASFLHRESIRDRNGAAIRLPVLAPPG
                     SVFTYGPSHLQIFSELLRRKLNGRSTVSFIKQQLLDPLRLSDLEFKKDRRGNPLPASG
                     FELTAREWARLGELVLGRGNYHGRHIVSPDLLRQAFTGSNANPSYGFTFWLNAQAPHA
                     REVDMEKEIDLAWERAGWSGTCICTAAPPDTVVALGSGYQRLFIIPSLRALIVRQGWD
                     SKFSDAYFLRLVLGR"
     gene            4359..>4984
                     /locus_tag="VFO30_07695"
     CDS             4359..>4984
                     /locus_tag="VFO30_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9419208.1"
                     /translation="MNKFAIGCGGFIVILLFLVLVVGLFFGGTYNRLVRLQQSVDQQW
                     AQVQNVYQRRADLIPNLVNTVQGAANFEKSTLVEVTNARASVGRVQLDPNKAPTDAAQ
                     LEQFQAAQGQLSNALSRLLVVVERYPELRANQNFLSLQAQLEGTENRISVERGNFNSA
                     VQTYNTAVRSFPTNLIAGMFGFSPRPFFAAQPGAERPPPVQFNFGSPAP"
BASE COUNT         1091 a         1483 c         1424 g          986 t
ORIGIN      
        1 gcaaacaccg ccaccggctg gcaaacgctg tttaacaaca ccggcgacaa caacacagcc
       61 gacggtgcct ttgcgctctt tagcaacacc accgggcatg ataatacggc cgatggtttt
      121 ggggcgctct ctagcaacac caccggtcat agaaacgtgg ccaacggtga atttgcgctc
      181 tatagcaata ccaccggcgc attcaacacg gccaacggcg ttgacacgct ctatagcaac
      241 acaaccggca acaacaacat cggcttgggc aatagcgccg gcggcgcgct aaccacgggc
      301 aattacaata tcgacatcgg caacgaaggt gttgctggcg aatcccaccg catccgcatc
      361 ggcacgcaag gaactcacaa acggacattc atcgccggca tttacggggt cgccgccgct
      421 caaggcgtgg cggtctatgt gaattcatcc gggcaactcg gcacgctcac ctcctccgcg
      481 cgcttcaagc aagacatcca ggccatggat gatgccagcg acgcgctcct gtcgctgcgg
      541 ccggtgacgt tccgctacaa aaaagaaatt gatcctgaag gcatcccgca gttcggcctc
      601 gtggccgagg acgtggcaaa ggtgaacccc gatctggtgg ctcgcgacgc gcagggggag
      661 gtttacaccg tgcgctacga ggcggtgaac gcaatgttac tgaacgagtt cctcaaagag
      721 caccgacaag tgcagaaaca acagaagcaa attgaaaacc tcaccgcgca gttgaaagac
      781 caggctgcgc aaatccgaaa ggtcaacgat aaggtggaaa tgaataagcc ggtgccgcaa
      841 atggtcctca acaatcagtg accgcagcag tagatcgata atttgccgaa agtttcccgt
      901 ttacgtattg ccttcgtccg tttcaattag cacgtgacgc aagcttgttg tcactctgag
      961 cgcgcggaag aatctcacat tcgcacgagg atccgcttgc ttggagtgac ttgagatgtg
     1021 tccgaaaatg cccggggaaa gtttccgttt tacgaaccgc ccttctagcg ttgaatttag
     1081 ctcgtgacgc cggcgaagaa gattttcggc accgatggcg tgcgcggtgt cgcaaatgta
     1141 gaaccggtca cggccgagac cgcgctcaag ctgggccgtg ccgcggcgca cgttttcact
     1201 cagcttaacc cgcgcaccat cccggaaggt acgcgcccga agatcgtcct cggcaaagac
     1261 acgcggctct ccggttacat gctagagaac gcgctcgttg cgggaattac gtcgctcggc
     1321 gtcgacgttc tcatcattgg cccgcttccc acgcccggcg tcgcttacat cacgcgctcg
     1381 ctccgcgccg acgcgggtat tgtcctctcc gcctcccaca atccgtacga ggataatggc
     1441 atcaaatttt tccggcacga cggctacaaa ctcgacgacc aggtcgaaga gcagatcgag
     1501 cagctcgtct tcaccggcaa gatcgacaat gtgcggccga cggccggaaa aatcgggcgc
     1561 gccgcgcgga ttgacgacgc gctcggccgc tacgtcgaat tcgccaaggc gagcttcccg
     1621 aaaaaaatgt cgctcgaagg gatgcgtgtc gccatcgatg tggcgaacgg cgcggcttac
     1681 aaatcgacgc catgcatatt acgcgagctc ggcgccgaag ttgccgtggc gcacaacgag
     1741 ccgaacggca tgaacatcaa tgatgcctgc ggcagcacgc atccggagga aattcaacgc
     1801 atcgtaaata ttagcggggc ggacgtgggc atcacgcacg atggtgatgc cgaccgtgtc
     1861 cttctctgtg atgagaacgg cgagattgtc gatggcgacg agattctcgc gatcgcgggg
     1921 ctcgatcttc tcaataacgg ccaactccga gacaagacat tggttgcgac ggtcatgagc
     1981 aacttcggtc tggatgaaac catcgccgag cacggtggca aagtcgtgcg cacgaaagtg
     2041 ggcgaccgtt acgtgattga ggacatgctc gcgcgcaatc tgaatctcgg cggcgaacag
     2101 agcggccaca tcattttccg ggatttcacg accacgggcg acggcattat cagcgcgctg
     2161 cagatcttgc gcattatgca tgcgaccggg cagccgctcg gcaaattgag aacgtgcctg
     2221 aaaaaatatc cgcaggcgca gcgcaacctg gaggtgaaag cgaagccgcc gatcgaagat
     2281 ttgccggagg ttatgaaatt gcgcgatgaa atcgagcgcg aactcggcgg caaaggccgg
     2341 atcttgctac gatattcggg gaccgaaccg aagatccgcc tgctgatcga aggccgcgag
     2401 ttcgacaaga tcgacaagca ggccaaccgc atcgccgatg ccatccaaag cgcaatcggc
     2461 gcaaactaaa tgacactggg ccgcgtcgtc gaagcacttc ttttcagcgc acaaaaaccg
     2521 ctttcaacaa aagagattct cgacctttta cggcgggcca gcggcagtga cgaattctcg
     2581 cctaacgaat ttgccaaagt gcgcgaggct gacgtcgcgg cggcacttca ggaaatcaag
     2641 attgagtaca tcgaacaaca gcgcgcgttt cagctggtcg aaaaggtcga cggctggcag
     2701 cttgcgaccg atccgcagta cgcgcgttgg gtgcgggagc tttttccggc accgcgcccg
     2761 gcgcggttga gcgcgccggc tttggaaacg ctcgccatca tcgcttaccg tcagccgatc
     2821 acacgcgcag acgtcgaggc ggtccgcggc gtcagcatcg atggcgtctt gcaaactctg
     2881 atggagcgcg ggttggtgaa aatcggcggg cgcgcagaca tcccgggtcg gccgttgctt
     2941 tacgagacga cccagttctt tctcgatcat ttccggttgc ggcatctcga tgagctgccg
     3001 aatgtcgaag agctgagaaa acgatatttg ccagtggcgc cgcgagcctc tgtagccgcg
     3061 gtcgatgacc gcggcccggc atcagcaacg ccggctgcaa ctaatctgta gccgtcgccc
     3121 ggtgggcgac gcaacgcaca gatgaatggt tcgtctcggt cgcgctttgc acagcgaagc
     3181 ggttacagca tcctcgcgct cgcattcgta acatcgacag ttgcattcgg tgaaatcaag
     3241 ccgagcgatt gcgcgcgcgc cgcaaaatat tccgacagcc ggcgcggcat gtccatgctc
     3301 gtgatgcaaa atggtcgcac cgttttcgag cattatgccc acggtggcat gcctgaactt
     3361 cgctcgccta ttttcagtgg cacgaaaagt ttctggggca tcgcggcgct cattgccgta
     3421 caacgccggc tctttcagct cgacgaccga gttgctgaca cgattaccga atggaagaac
     3481 gatccgggca aatcgcagat gacaattcgt gagctgctta atttcaccga tggaatcgaa
     3541 ggagcgtcat ttttgcaccg cgaatccatc cgcgaccgca acggcgccgc gatccgcttg
     3601 ccggtactgg ctccgccggg ctcggtcttc acatacgggc ctagccatct ccagattttc
     3661 tccgaattgt tgcgccgtaa gttgaatggg cgctcgacgg tttcattcat caaacaacag
     3721 ctgctcgacc cgctgcgtct ctctgatctc gagttcaaaa aagaccggcg cggcaatcca
     3781 ctccccgcat cgggctttga attaacggca cgggaatggg cgcgcctcgg cgagcttgtt
     3841 cttggacgcg gtaactatca tggccggcac attgtgtcgc ctgatttgtt acgtcaggcg
     3901 ttcaccggct caaatgcgaa cccatcttac ggcttcacat tttggctgaa cgcgcaggcg
     3961 ccgcatgccc gtgaagtcga catggaaaag gaaatcgatc tcgcgtggga acgcgctggc
     4021 tggagcggca cttgcatttg cacggccgcg ccgcctgaca cggtggtagc gctcggttcc
     4081 ggctatcagc gcttgtttat tatcccatcg ctgcgtgcgc tcatcgtcag gcagggctgg
     4141 gattcaaaat tttccgacgc gtatttcctc cggctcgtac ttggacggta ggtgggtccg
     4201 gccgcgccgc gggcgatgtt aacctagtgc ggctccgccg ccgtttatac cgcgttggga
     4261 aaacgcgatc cacctgtgct ccgcttctct gtcgagagcc gcgattgact tggtaccgca
     4321 tttcctcaac cattgccgcg gcgtcagata tcgatcttat gaacaagttt gccattggtt
     4381 gcggcggttt catcgttatt ctgctcttcc ttgtccttgt cgtcggtcta ttttttggcg
     4441 ggacttacaa ccggctcgtg cgcttgcagc agagcgtcga ccaacaatgg gcgcaggtgc
     4501 aaaacgttta tcagcgccgc gctgatttga ttcccaacct ggtcaacacc gtgcaaggcg
     4561 cggctaactt tgagaaatcg acgcttgtgg aagtcaccaa cgcgcgcgcc agtgtcggcc
     4621 gagtgcagct cgacccgaac aaagcgccca cggacgccgc gcagctcgag cagtttcaag
     4681 cggcacaggg ccagctgagc aacgcgctct cccgtttgct cgttgtggtc gagcgttacc
     4741 cggaactgcg ggcaaatcag aacttcctta gtttgcaggc gcaactcgaa ggcaccgaaa
     4801 accggatctc ggtcgagcgc ggcaacttca acagcgccgt gcaaacctac aacactgcag
     4861 tacgcagctt cccgaccaac ctgattgccg gaatgttcgg cttttcgccg cggccattct
     4921 ttgccgccca gccaggcgcg gaacgaccgc ccccggtgca gtttaacttc ggttcgccgg
     4981 cgcc
//
LOCUS       DASTDG010000231         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_56403, whole genome shotgun sequence.
ACCESSION   DASTDG010000231 DASTDG010000000
VERSION     DASTDG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56403"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFO30_07700"
     CDS             complement(<1..1172)
                     /locus_tag="VFO30_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET9419209.1"
                     /translation="MSATLDDLREKIERAVAGGTLLDSAANNMRALLSGTPSAIYVRS
                     VEELVAADQWNELNDRFYKTLEFGTGGLRGRTIGKIVTTAERGHATKSDRPQFPCVGT
                     NVMNFYNVSRATQGLVAYIKEWNARERGIGFQPINTKNRQDADATKPSIVIAHDTRFF
                     SKEFMELAAKIASENGCAGFVFDGPRSTPELSFAVRYLNASAGAVITASHNPPHDNGY
                     KVYFADGAQVIAPHDRGIIEKVNAIASETYEPLPTDRQGKVTTLGAEVDEAYMKRLES
                     IVLDRRVLEDAKSLRVVFTPLHGTGAITLRPMLKRLGFNFEIVPEQEKSDPRFSTVKS
                     PNPESAEALTLGIELAKKTNADIVIATDPDADRLGVAVRGVQGEMKLINGNQIGSLL"
     gene            1281..>2732
                     /locus_tag="VFO30_07705"
     CDS             1281..>2732
                     /locus_tag="VFO30_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419210.1"
                     /translation="MDPRKFFAELKRRNVYRAAVLYGMGSWLLTQIATQVFPIFDISN
                     SIIRLMVIALAAGFPIAMLVAWLYELTPEGFVRTEDVDLARQKGIGRKWDFVIIGVLL
                     LVIVMLVYQRFPFRTESGEVIPEKSIAVLPFDNFSDDKENAFFADGIQDDILTSLAEI
                     GGLRVISRSSVMQFRDAAARNLREIGQRLGVATVLEGSVRRAGDRVVINVQLIDALHD
                     RHLWANRYDRALVDSLGLQGELAAEIAEALRVTLSPKEKARVQTKLTANPDAYLFYLR
                     ANQIERNPDTLLQDYKKAEQLYLQAIALDPKFALAHARVASTRAAIFHYFEPLDSWKA
                     GARAEAETALRLQENLAEAHLALGHCIYWIDQNYDRALDEFDIAARLAPNNSEVDGLI
                     AAIKRRQGKWQESLQTYEKINKLDPQNPNIVRNLLFTHTALRQWPEAARVAERMRTMA
                     PASLVAKIQSDYVDFWWKGDTRLLESLLDQIPAG"
BASE COUNT          543 a          788 c          791 g          610 t
ORIGIN      
        1 agcagcgagc cgatctgatt tccgttaatc aacttcatct cgccttgcac accgcgcacc
       61 gccaccccga gccggtccgc atccggatcg gtcgcgataa caatgtcggc gtttgttttt
      121 ttggcgagct caatgccgag cgttaacgct tctgcgcttt cgggattcgg cgatttcacc
      181 gtggaaaatc gcggatcgga tttttcctgc tccggcacga tttcgaaatt gaatccgagc
      241 cgctttaaca tcgggcggag cgtgatcgcg ccagtgccgt gcagcggcgt gaagacaacc
      301 cgcagcgact ttgcgtcttc gagcacgcgc cgatcgagaa caatcgattc aagccgcttc
      361 atgtaagctt catccacttc ggccccgagc gtcgtgactt ttccttgtcg atctgtcggc
      421 aacggctcgt aagtttcact cgcgatcgcg ttcacttttt caatgatccc gcgatcgtgc
      481 ggcgcgatca cttgcgcgcc atcggcgaag taaaccttgt agccattgtc gtgtggcggg
      541 ttgtggcttg cggtgatgac agcgccggcg ctcgcgttca agtagcgaac tgcgaaggag
      601 agttccggcg tcgagcgtgg tccatcgaat acgaaaccgg cgcacccgtt ctcggatgcg
      661 atcttcgcgg ccagctccat gaattccttg gaaaaaaatc gcgtgtcatg cgcgatcaca
      721 atgctcggtt tcgtggcatc ggcatcttgc cgattttttg tattgattgg ctggaagcca
      781 atgccacgtt cgcgcgcgtt ccactctttg atgtacgcaa ccaggccctg tgttgcgcga
      841 ctcacgttgt agaaattcat cacattcgtg ccgacgcatg ggaactgtgg gcggtctgac
      901 ttcgtcgcgt ggccgcgctc tgcggtcgtg acgattttcc caatcgtgcg cccgcgcaga
      961 cctcccgttc caaactcgag cgttttatag aaccgatcgt tgagctcatt ccattgatcg
     1021 gctgcgacga gctcctcgac cgagcgcaca taaatagccg acggcgtgcc ggagagcagg
     1081 gcccgcatgt tgtttgctgc cgaatcaagc aacgtgccgc ctgccactgc gcgctcaatt
     1141 ttctccctca aatcatcgag tgtggcggac atggcaggcc aactttccga cgaaacgcgg
     1201 ctttgcaagc tggcctgctc cggcagcgcc ctaaccgagg agttgaaccg ctcgataccg
     1261 tgtggcacaa cggcccagcg gtggacccaa ggaaattttt tgccgagctc aagcggcgca
     1321 atgtttatcg cgcggcggtg ctttacggga tgggatcgtg gctgttgacc cagatcgcga
     1381 cccaggtgtt cccgattttc gacatttcca attcgatcat ccgcttgatg gtcatcgcgc
     1441 tcgccgccgg ttttcccatc gccatgttgg tcgcgtggct ttacgagctg actccggaag
     1501 gattcgtccg gacggaagat gtcgatcttg ccaggcaaaa aggcatcgga cggaaatggg
     1561 acttcgtcat cattggcgta ctcttgctag tgatcgtgat gttggtttat caacggtttc
     1621 catttcgcac tgagagcggc gaagtgattc cggaaaaaag catcgccgtc ctgccgttcg
     1681 acaactttag cgacgacaag gaaaacgcct ttttcgccga cggtattcag gatgatatcc
     1741 tgacgagtct ggccgagatc ggcgggctgc gagtaatcag ccgttcgtcg gtgatgcagt
     1801 ttcgcgatgc ggccgcacgt aacctgcgcg agatcggcca gaggctggga gtggccactg
     1861 tgcttgaggg cagtgtccgg cgcgcaggtg accgcgtggt catcaacgtc cagctgattg
     1921 atgcgctgca cgatcgtcat ctctgggcca atcgctacga ccgcgcgctg gtcgattcgt
     1981 taggcctgca gggtgaattg gcggccgaga tcgccgaagc gctgcgggtg acactgagcc
     2041 cgaaggaaaa agcgcgcgtg caaacaaagc tgacggcgaa tcccgacgct tacctgtttt
     2101 atctgcgcgc aaaccaaatc gaacgcaatc ccgacacgct cttgcaggat tataagaaag
     2161 ccgagcagct ctacctccag gcgatcgcgc tcgacccgaa gtttgctctt gcccatgccc
     2221 gggtcgcctc cacccgcgct gcgattttcc actatttcga accactcgac agttggaagg
     2281 cgggtgcgcg cgccgaagcc gagactgcct tgcgattgca agaaaatctg gcggaagcgc
     2341 acctcgcact cggtcattgc atttactgga tcgaccagaa ttacgatcgt gcgctggacg
     2401 aattcgatat cgcggcgcgt ttggcgccga acaatagcga ggtggatggg ttgattgctg
     2461 cgatcaagcg ccgtcaggga aagtggcagg agtcgctcca aacctatgag aagataaaca
     2521 agctggaccc gcagaacccg aacattgtcc gcaatctcct cttcactcat accgctttac
     2581 gtcagtggcc cgaggccgcg cgggtagcgg agcggatgcg gacaatggcg ccggcctcgc
     2641 tcgtcgccaa gatccaaagt gattatgtcg atttctggtg gaaaggcgac acgcgtttgc
     2701 tggagtcgct gctcgaccag attccggccg ga
//
LOCUS       DASTDG010000232         9162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_56484, whole genome shotgun sequence.
ACCESSION   DASTDG010000232 DASTDG010000000
VERSION     DASTDG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56484"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFO30_07710"
     CDS             <1..1102
                     /locus_tag="VFO30_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9419211.1"
                     /translation="LRQPVRAAAHEINRHRRDQNGFVFAVDVPTGLDSDSGEVDPEDC
                     VTADVTVTIGFAKHGLIVDQALDYVGRLEIVPLPELRAGVASPRELVASADSLRPLVP
                     PRKFSAYKNQFGRIGIVAGSRGFIGAALLTTQGALRGGAGLVELFVLEDIYPIVASMA
                     PIEAMVKPVKKYPQLLHHEIAAWALGPGLGHSRGPQILELIRCAKQPMIVDADGLNVL
                     SGKIDILHRAKGARLLTPHAGEMKRLFDTSKMPRSAVARKFCEEFPVTLLFKGSRTIV
                     AERTKPLSYNTTGNPGMASGGMGDVLTGVCAALVGQGLSLYDAARVGAWACGRAAEMS
                     IFHSGESEESLLARDVLDHLGPAFHELRASAE"
     gene            1107..1982
                     /locus_tag="VFO30_07715"
     CDS             1107..1982
                     /locus_tag="VFO30_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9419212.1"
                     /translation="MVYPYETAGLETTARAKNSWWARLKKTLGPLAVAGAVIAKFFAK
                     LKFILLPLLKFLPILLKSGGTMLLMIWVYTAIWGWQFGVGFVLLLLVHETGHLLVAKK
                     FGLNVGAPVFIPFMGAFIALKEAPRNAWMEACVGIGGPMLGTFGALVCNLLGEMFGAP
                     VFVALAWFGYFLNLFNLTPVGMLDGGRIVSAMSRWLWLPGFAGLLWFGWKFPNFIVWL
                     IIFLSLPRIASLFRRRTEEERRYYEVTAAQRWTMSILYFGLILSLVAGMHLAQNDLHE
                     RGVRAHGKGRDVIVQ"
     gene            complement(2003..3040)
                     /locus_tag="VFO30_07720"
     CDS             complement(2003..3040)
                     /locus_tag="VFO30_07720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9419213.1"
                     /translation="MRRGFLAGAGFSFLLLARSFGQQVEVVRQSPPQPVAPPALSGVD
                     EEVKERASEALPSAVHYNRPAAPMLTAEQMRKAGALARQKIENEKRLQADRAADAAKR
                     FLAASQPPQDRRSRISEPALSHAINPRAETAFTKLANGFDFPIGKPDAQGYYKARGFR
                     SHGHLGEDWDGIRGGDTDLGDPIYSIGNGVVVFARDCHMGWGNVVIIRHAFREDGVVK
                     NIDSLYGHLNTILVQRGQAVSRGENIGTMGTAHGIYDAHLHFEIRKNLEIGMSRAAFA
                     RDLSNYYDPTQFILSHRQLPARGGYRIAMNTFTRDANIHWNERGNYSQSSSRGTSESA
                     TALRRALASQH"
     gene            complement(3194..3610)
                     /locus_tag="VFO30_07725"
     CDS             complement(3194..3610)
                     /locus_tag="VFO30_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419214.1"
                     /translation="METDIILPDLQSCVLCEDVRCEFNGMQTLVGVINVIPAPALPVN
                     WMRLCIWARWCSGSGKFRQRSRIVGVDEQQVIAQAEVEFVLREMEGHATNVHYFGGVQ
                     FQQYGVHHVEIYLGDELRLRFPLPVVRVTPPKQMQS"
     gene            complement(3611..4480)
                     /locus_tag="VFO30_07730"
     CDS             complement(3611..4480)
                     /locus_tag="VFO30_07730"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9419215.1"
                     /translation="MGDFLLSILLGIVEGVTEFLPVSSTAHLRIAEAVLRLDLHDSFW
                     KMYTIVIQLGAILALPVYFWQRILKFLSTFPQGERGDRNLLTHPLSLTLIAFVVTAVP
                     SWALTKQIGKNLESLWVMATALFLGGIVMWIVDLMFTRPRVIHMEQMSLPQAIWIGAA
                     QILSAVFPGTSRSMATIAAGQVAGMSRPAALEFSFFLSMPTMLVATGYDFLKTITPNR
                     RTELEGNIAPLAVNTHQWIVLAIGFVVSFVVALLVVAWFMRWVRNRGFAPFAIYRIAL
                     ATVISILLVRGVV"
     gene            complement(4520..4858)
                     /locus_tag="VFO30_07735"
     CDS             complement(4520..4858)
                     /locus_tag="VFO30_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9419216.1"
                     /translation="MKKIEAIVKPFKSEAVKDALTEIGIEGMTLSEVKGFGRQKGHSE
                     IYRGSEYTVDFLPKVKFEIVVTDDRAQRAVEAILTAAKTGKIGDGKVFVSDVELAVRI
                     RTDERGGEAV"
     gene            complement(4918..6717)
                     /gene="mutL"
                     /locus_tag="VFO30_07740"
     CDS             complement(4918..6717)
                     /gene="mutL"
                     /locus_tag="VFO30_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9419217.1"
                     /translation="MSRIRLLTDAVASQVAAGEVVERPASVVKELIENSIDAGAGKID
                     VLIRRGGISLIRVIDDGCGMDRDDALLSLERHATSKIRDVNDLSAVATLGFRGEALPS
                     IASVSRFRLTTREPQAVAGTEIVVNGGKIDIVRDGGEAPGTQLEVRSLFYNLPARRKF
                     LRSENTEGRNIEHQVQLQAIGHPHIAFTLTRDDRLVFQLPPAVSLIDRVRDLYGSELL
                     ERLLPVNDATRHIEISGLIGQAGLSRQTRAQQLVFVNGRAIESALVNSALREGYHTAL
                     MKGQFPVTFIFLTLEPALVDVNVHPAKREVRFRDPNGVREAIVRAIQQTLARGRTDWQ
                     EKFRAPAPIGATISVSPTAAPAVPRELPQLEREFALRADRSVTGQAHRLPAQPARSAG
                     TTPAPQSAPPAQSKPQQFQIIGVLSKLYVLMENADGLVLVDQHAAHERILFEELRRRM
                     EEQGVPSQKLLLAQTFDLPARDAEWVERNMSILQKMGIGIESFGPNTFKIDSVPAFLR
                     ASEPVTFMQHVIDDLKSASNSSSPMRLGEEIIAKTVCRHAVKANDPLRYLEVEKLIQD
                     LLDCDLPYCCPHGRPTMIQISLTELEKKFGRKV"
     gene            6830..7165
                     /locus_tag="VFO30_07745"
     CDS             6830..7165
                     /locus_tag="VFO30_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9419218.1"
                     /translation="MINVTQNAARQLRALLEQQGDPAGKGLRVHVAKGGCSGLHYEMS
                     LDEKQAGDKVVQHDGVEFFIDSDSAEILRGATLDFHDGLTGVGFQIVNPNASRTCGCG
                     SSFEAAPTH"
     gene            7195..8832
                     /locus_tag="VFO30_07750"
     CDS             7195..8832
                     /locus_tag="VFO30_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419219.1"
                     /translation="MAYSAFQYEEPYARPRRRINYFAWTVAILLLTGLALAAWLGSFY
                     IFDQPERPDSYRILQRLHKLEPPKRFELTFAPSGEFLNAKQLFDRYVAMGTAELAKMN
                     AELLRNYIRNYQAVRGLVPYVTGRYMTMEARELGPGDVFTSGMVALTGAVDHGELLME
                     HLYPSDPAALPLMKETLKMGLEIKLERTHDLSAVIHAERLADGRIMITAVPLLYGTYT
                     VTRGPGTFRLDPPFALNLAAGWPLFKEPMRLRAERHYVEYRQQPTGEHGPVPIPGMTP
                     NAPASAANELVRVEPAKPLALPPPPIAQPAAKGDKLAKAAASPKRGKLASKQKAAAQA
                     PSPPPPPPASPAPTIVTKRALPVGPSAAAVAAVSSALPQVASPIPAPSASAPPAVALA
                     SPPTIAAAQTASPPPNAAPEQAAGALASTAGGGTWKTFPAGKMPLGRLIGTGDLNDVA
                     ERGVTGERVYLRGQFVVNFSDANRAVLRPRAKLTDKVLHLGGGSNTRIIVEFPTGYTP
                     PPPGSVVMRDEARPYEITEVRKTEDGQLNVFAREIMQ"
     gene            8920..>9162
                     /locus_tag="VFO30_07755"
     CDS             8920..>9162
                     /locus_tag="VFO30_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9419220.1"
                     /translation="MIKHRPHRLDQIFVDQPLFFVTFCARNRRKLPSLDCAKAALETY
                     AMRGHTEFNVAVGRYVIMPDHIHLFVRGGPDFVLSTW"
BASE COUNT         1806 a         2791 c         2662 g         1903 t
ORIGIN      
        1 gttgcgccag ccagtgcgcg ctgccgcgca cgagatcaat cggcatcgcc gcgatcaaaa
       61 cggattcgtt tttgccgtgg atgttcccac cggactcgac agcgattccg gcgaagtcga
      121 tccagaagat tgcgtcactg cggatgttac cgtcacgatt ggttttgcca aacatgggtt
      181 gattgtcgat caagcgctcg actacgtcgg ccgtcttgag atcgttccac ttcccgagtt
      241 gcgcgccgga gtggcttcgc cgcgggaact tgtggcatcg gcggattcgc tgcgcccgct
      301 cgtgccacct cgcaaattca gcgcttacaa aaatcagttc ggccgcatcg gcatcgtcgc
      361 tggctcacgc ggcttcatcg gtgccgcgct tttgacgacg caaggcgcgt tgcgaggcgg
      421 cgccggtctc gtcgagctct tcgtcctgga agacatctac ccaatcgtag cgagcatggc
      481 cccgatcgag gccatggtaa agccggtgaa aaaatatccg cagctcctcc atcacgagat
      541 cgcggcgtgg gcgctcggcc ccggccttgg tcactcgcgc ggcccgcaaa ttctcgagct
      601 gatcagatgc gccaaacaac caatgatcgt ggacgccgat ggactgaatg tgctctcagg
      661 caagatcgac atcttgcacc gcgccaaagg cgcgcgtctt ttgacaccgc acgctggcga
      721 gatgaaaagg ttgttcgata ccagcaaaat gccgcggtcc gctgtcgcga ggaagttttg
      781 cgaagagttt ccggttacac ttttattcaa aggcagccgg acaatcgtcg cggagcgcac
      841 gaaaccgctg agttacaaca caaccggcaa cccgggcatg gccagcggcg gaatgggcga
      901 cgtgctcacc ggtgtctgtg ccgcactcgt cggccagggc ttatcgcttt acgatgcggc
      961 gcgcgttggc gcgtgggctt gcggtcgtgc cgcggagatg tcgatctttc attccggcga
     1021 aagcgaagaa tcgctcctgg cccgcgacgt tctcgatcac ctcggtccgg ccttccacga
     1081 actgcgcgcc tcggccgagt agcgaaatgg tttatcccta cgaaactgcg ggactcgaga
     1141 cgaccgctcg agcgaaaaac agctggtggg cgcgacttaa aaaaacgctc ggcccacttg
     1201 ccgtcgccgg cgcggtcatc gcgaagtttt tcgcgaaact gaagttcatc cttctcccgc
     1261 tgctcaagtt tctgcccatt ctgctcaagt ccggtggcac gatgctgctg atgatttggg
     1321 tttacacggc gatatggggt tggcagttcg gcgtcggatt cgtcctgctc ttgctcgtcc
     1381 acgaaaccgg tcacctgctg gtcgcgaaga aattcggact gaacgtcggc gcgccggtgt
     1441 tcatcccgtt catgggcgcg ttcattgcct tgaaggaagc gccgcgcaat gcttggatgg
     1501 aagcgtgtgt tggcattggc gggccgatgc tcggcacctt tggcgcactc gtctgcaatt
     1561 tgttaggtga aatgttcggt gcgccggtat ttgtcgctct cgcctggttc ggctatttcc
     1621 tcaacctatt caaccttacg ccggtgggca tgctcgatgg cggccgcatc gtcagcgcaa
     1681 tgtcgcgatg gctgtggctg ccgggctttg ccggcctgct ctggttcggc tggaaatttc
     1741 cgaacttcat cgtttggctg atcattttcc tgtcgctgcc gcgaatcgct tcgctttttc
     1801 gccgaagaac ggaagaggaa cgccgctact acgaggttac agcggcgcag cgttggacaa
     1861 tgtccatctt gtatttcggc ctgattctct cgcttgtcgc tggcatgcac ctcgcgcaga
     1921 acgatcttca cgagcgcggt gtgcgcgctc acggcaaagg ccgcgacgtg atcgtgcagt
     1981 aggcggcaaa gctggcggct ggtcagtgct gcgaagcgag tgcgcgcctt agcgccgtcg
     2041 ccgattcgct cgtgccgcga ctgctcgact gcgagtagtt acctcgctca ttccagtgaa
     2101 tgttcgcatc gcgggtgaaa gtattcatcg caatgcggta cccgcctcgt gccggtaact
     2161 ggcgatgcga taaaatgaat tgcgtaggat cgtagtaatt gctcaggtcg cgggcaaacg
     2221 cggcccggct catgccgatc tcgagatttt tgcgaatctc gaaatgcaaa tgcgcgtcgt
     2281 agatgccgtg cgccgtaccc atggtgccaa tattttcccc acggctgacg gcctgtccgc
     2341 gctgtaccag gatggtgttc aggtgaccgt agagtgagtc aatgttttta accacgccat
     2401 cttcacgaaa ggcgtgacgg atgatgacca cgttccccca gcccatgtga caatcgcgcg
     2461 caaacacaac tacgccattc ccgatgctat agatcggatc accgaggtcc gtgtcgccac
     2521 cgcggatacc gtcccaatcc tcgccgagat gtccgtgcga acggaagccg cgcgctttgt
     2581 agtagccttg cgcgtccggt ttaccgatcg gaaaatcgaa gccgttcgcg agttttgtaa
     2641 acgcagtctc ggcacgcgga ttgatggcgt gcgacaaagc cggttccgag atgcgcgatc
     2701 gtcgatcttg cggcggttgc gaagctgcga gaaatcgttt cgccgcgtcc gccgcacgat
     2761 ccgcttgcag acgcttttcg ttttcgattt tctgccgcgc gagcgcgcca gctttgcgca
     2821 tctgttcagc cgtaagcatc ggcgccgccg gccggttgta gtgcactgcg ctgggcagcg
     2881 cctcagaagc tcgctcctta acttcctcgt caacgccaga aagcgctggc ggggccaccg
     2941 gttggggcgg gctttgacgc acgacctcga cttgttgccc gaaactccgg gcgaggagca
     3001 ggaagctgaa accagctccg gcgaggaatc ctcttcgcat aagaagcgct cgttttaacg
     3061 aaagggtctt cgcctcgcaa gcacttattc acacccatgc atagcacacg tgggtgtcat
     3121 cccgaacgtt gtgaggtccg cgcccgtgtc cgcggctcgg gatgacagcg cgagggtgga
     3181 gtgctattga cgattaggat tgcatttgtt tcggtggcgt cacgcggacc accggcaatg
     3241 gaaagcgcag gcggagttca tcaccgagat aaatctccac gtgatggacg ccgtattgct
     3301 ggaactgcac gcccccgaag taatgcacgt tcgtggcgtg tccttccatt tcccgaagca
     3361 cgaattcgac ctcggcttgg gcaatgactt gctgctcgtc cactccgacg atgcgcgagc
     3421 gctgccggaa ttttcccgag ccgctgcacc agcgggccca gatgcagagt cgcatccagt
     3481 tcaccggcag cgcgggcgca ggaatgacgt tgattacccc gacgagcgtc tgcatgccgt
     3541 taaattcgca gcgcacgtcc tcgcagagga cgcagctctg gaggtccggc aaaataatgt
     3601 cggtttccat ttagaccacg ccgcgcacca gcaagatcga gatcacggtc gcgagtgcaa
     3661 ttcggtaaat ggcgaaaggc gcaaaaccgc ggttccgcac ccagcgcatg aaccacgcga
     3721 cgacaagcag cgcgaccacg aaggaaacga caaagccaat cgcgaggaca atccactgat
     3781 gcgtatttac cgctagtggc gcgatgttcc cctcgagctc ggtccgccga tttggcgtga
     3841 tggttttcag aaaatcgtat cccgttgcga cgagcatggt cggcatggac agaaaaaacg
     3901 aaaactcgag cgcagcgggg cgcgacatcc cagccacctg accggcggcg atggtcgcca
     3961 tggagcgcga cgtgccggga aaaacagcgg aaagaatctg cgccgcccca atccagattg
     4021 cctgcggcaa gctcatctgc tccatgtgaa tgacccgtgg ccgcgtgaac ataagatcga
     4081 caatccacat gacgatgccg ccgaggaaca gcgccgtggc catcacccag aggctttcca
     4141 ggtttttacc gatctgcttc gtcaacgccc acgaaggaac cgcggtgacc acaaacgcga
     4201 tcaacgtcag gctcaacggg tgcgtgagaa gattgcggtc accccgctcg ccttgcggaa
     4261 acgtgctcag gaacttcaga atgcgctgcc aaaaatacac cggcaacgcc agaatcgcgc
     4321 cgagctggat cacgatcgtg tacatcttcc agaaactgtc gtgcagatcg agacgcaaaa
     4381 ccgcttccgc gatacgcaaa tgagcggttg agctaaccgg cagaaattcc gtcactcctt
     4441 cgacgatccc aagcaagatc gacaacagaa aatcacccac gcgcgttttc tactttctaa
     4501 tttctacctt ctactttttt cagaccgctt ctcctccgcg ttcgtcggtg cggatccgaa
     4561 cggccagctc cacgtcggag acaaagacct ttccatcgcc aatttttccg gtcttcgccg
     4621 cggtcaggat tgcctcaacc gcgcgctgcg ctcggtcatc ggttaccacg atttcgaact
     4681 tcaccttggg cagaaaatcc accgtgtact cgctgccgcg ataaatttcg ctgtgacctt
     4741 tttgccggcc gaagccttta acctcgctca acgtcatgcc ctcaatgcct atttcggtca
     4801 gtgcgtcttt cactgcctca cttttgaatg gcttgacgat ggcttcgatt tttttcacct
     4861 cggccagatt agaagtgcgc ctcgaaatgg caagctttgt gaccgcaggc tccgcggtta
     4921 gactttgcgg ccgaattttt tctccagttc ggtgagtgag atctggatca tggtcggccg
     4981 gccgtgcggg cagcaatatg gcaaatcgca gtcgagcaaa tcctggatga gtttttccac
     5041 ttccaggtag cgcaacggat cgttggcttt gacggcatga cggcaaaccg tcttggcgat
     5101 gatttcttcg ccgagacgca tcggcgagct gctattgctc gcacttttca gatcgtcaat
     5161 gacgtgttgc ataaaggtga ccggctccga tgcgcgcagg aacgccggca cgctgtcgat
     5221 cttgaatgtg ttcggcccga agctttcgat gccgattccc atcttttgta agatcgacat
     5281 gttgcgctcg acccattcag cgtcgcgcgc gggcagatcg aacgtctgcg cgagcaagag
     5341 cttttgggaa gggacgccct gctcttccat gcgccggcgc aattcctcga agaggatccg
     5401 ctcgtgcgcc gcgtgctggt caacgagcac gagcccgtcg gcgttctcca tgagcacgta
     5461 gagtttgctc agcacgccga taatttggaa ttgctgaggc ttggattggg cgggcggcgc
     5521 agattgtggg gcaggcgttg tgcctgccga ccgcgcaggt tgcgccggca agcgatgcgc
     5581 ttgccccgta actgacctat cggcgcgcaa tgcgaactca cgctcgagct gcgggagctc
     5641 acgcggaaca gccggcgcag ctgttggtga cacggagatg gtggcgccaa tcggcgccgg
     5701 cgcgcggaac ttttcctgcc aatcagtccg gccgcgcgcg agcgtttgtt gaatcgcccg
     5761 cacgattgct tcgcgcacac cgttaggatc gcgaaagcgc acttcgcgtt tggccgggtg
     5821 cacgttcaca tccaccaacg ccggttcgag cgtgaggaaa ataaacgtga cggggaattg
     5881 tcctttcatc agcgcggtgt gatatccctc gcgtaaggcg ctgttgacca gcgcgctttc
     5941 aatggcgcgg ccgtttacga agacaagttg ctgcgcacgc gtttgccggc tcaacccggc
     6001 ctgcccgatc aagccgctga tctcgatgtg gcgggtcgca tcgtttaccg gaagcaaacg
     6061 ctcgagcaat tcgctgccgt aaagatcgcg gacgcggtca atcaagctca ccgctggcgg
     6121 aagctgaaag acgaggcgat cgtcgcgagt aagtgtgaac gcgatgtgcg gatgacctat
     6181 cgcctgaagc tggacttgat gctcgatgtt tcggccctcg gtgttttcag aacgcaaaaa
     6241 cttgcgccgg gccggcaggt tgtaaaagag cgatcggact tccagctgcg tccccggcgc
     6301 ttcgccgcca tcgcgcacga tgtcgatctt gccgccgttc acgacgatct ccgtgccggc
     6361 tacggcctgt ggttcgcgcg tggtaaggcg aaatcgcgag acactcgcga tgctgggcag
     6421 cgcttcgccc cgaaagccga gcgtcgccac cgcgctcaga tcgttgacat cgcgaatttt
     6481 gctcgtggcg tggcgctcga gcgagaggag cgcgtcgtcc cggtccatgc cgcagccgtc
     6541 gtcgatcacc cgaatgagcg aaatcccgcc gcgccgaatc aagacatcga tcttgcccgc
     6601 gccggcatcg atgctgttct cgatcagttc tttaacgaca gaggcgggac gctccacaac
     6661 ttcacccgcc gcaacctggc tcgccaccgc gtcggtcagt aatcggatcc ggctcatctc
     6721 gattgcaaat tgtagatttc cgatcaccga ttgcgaaaat aaaaggcggc ggcatgcgac
     6781 tagcagcgtc tttgacttgg gttagcccgg agttactttg gaattcgaga tgattaacgt
     6841 cacccagaac gcggcgcgac agcttcgcgc tctgctcgag caacagggcg atccagctgg
     6901 caaagggctg cgtgtgcacg tcgccaaagg cggttgctct gggttgcact acgaaatgtc
     6961 gctcgacgaa aaacaagccg gcgacaaggt ggtgcagcac gatggtgtgg agtttttcat
     7021 cgatagtgat agcgccgaga ttctgcgtgg cgcgacgctg gattttcacg acgggctgac
     7081 gggcgtgggt ttccagattg ttaatccgaa cgcttcgcgg acctgcggct gtggttcgtc
     7141 attcgaagcg gcgccgactc attgagcgcg cactatcttc tctctcccgc gtaaatggcg
     7201 tacagcgctt ttcagtacga agagccgtac gcgcggcccc gccggcgcat taactacttc
     7261 gcctggacgg tcgcgatttt gctcctgact ggtcttgccc ttgccgcgtg gctcggcagt
     7321 ttctacatct tcgatcaacc cgagcgaccc gacagttacc ggattctcca acggctgcat
     7381 aagctcgagc cgccaaaacg tttcgagctg acgttcgcgc cgagcggcga gtttctcaac
     7441 gcgaagcagc ttttcgaccg ctacgtcgcg atgggcacgg ccgaactcgc gaagatgaac
     7501 gcggaattgc tccgcaatta catccgcaat taccaggcgg tgcgcggtct cgtgccgtac
     7561 gtcaccggtc gttacatgac gatggaagcg cgcgagctcg ggcctggtga cgttttcact
     7621 tctggcatgg tcgcgctaac cggcgcggtc gaccacgggg agctgctgat ggaacatctt
     7681 tatccctccg accccgcggc gttgcccttg atgaaagaaa ccctgaagat gggcttggaa
     7741 atcaaactag aacgcacgca cgacctttcc gcagtcattc acgccgagcg cctggccgat
     7801 ggccggatca tgatcaccgc ggtgccgttg ctctacggga cctacaccgt tacacgcggg
     7861 ccgggcacct tccgactcga tccccccttt gcgctcaatc tcgccgccgg ctggcccttg
     7921 ttcaaagaac cgatgcggct ccgagctgaa cggcattacg tcgagtatcg acagcaaccc
     7981 accggcgagc acggcccggt gccaattccg ggaatgactc caaatgcgcc cgcatcagca
     8041 gcgaatgagc tagtgcgcgt agaacccgcc aaaccattgg cgcttccgcc accgccgatt
     8101 gcgcaaccag ctgcgaaagg cgataagctg gccaaagcgg ctgcgtcgcc gaaacgcgga
     8161 aagctggcca gcaaacaaaa agccgccgcg caagcgccct caccgccacc acccccgccg
     8221 gcgagtcctg ccccaacaat cgtcacaaaa cgcgcgttgc cggtcggacc aagcgctgct
     8281 gctgtcgctg ccgtgtcgtc agcgctgcca caagttgcct cgccaatacc tgcgccatcc
     8341 gctagcgctc ctcccgcagt cgccctcgca tcgccgccga cgattgcagc tgcgcagacg
     8401 gcttcgcccc caccgaacgc tgcaccggaa caagcggccg gtgctctcgc ttccactgct
     8461 ggcggcggca cgtggaaaac ttttccggca ggcaaaatgc cgctcgggcg attaattggt
     8521 actggtgatt tgaacgacgt agccgaacgt ggcgtgaccg gcgagcgcgt ctatctgcgc
     8581 ggacagttcg tcgtcaattt ttccgacgcg aatcgcgcgg tgctccgtcc gcgcgccaaa
     8641 ctgaccgaca aagtcctgca cctgggcggc ggctcgaaca cgcgcattat cgtcgaattc
     8701 cccactggct atacgccgcc cccgcctggt tcggtcgtca tgcgcgacga agcacgtccc
     8761 tacgaaatca ccgaagtccg caaaaccgag gacgggcagc tcaacgtctt cgcgcgcgaa
     8821 atcatgcagt gagctgtggc ggcaggcgtg ccgcctgcgt tttgttgaat tggtcgcagc
     8881 cgacacggct gcctccacag aagaggcaat ccaaagccag tgatcaagca taggccacat
     8941 cggctcgatc agattttcgt cgaccagccg ttgttcttcg tcacgttttg tgcgcgcaat
     9001 cgcagaaaac tgccgtcact ggactgcgcc aaagccgcat tggagacata tgcaatgcgc
     9061 ggccatacgg aatttaatgt tgccgtaggc cggtatgtca tcatgccaga ccacatccat
     9121 cttttcgttc gcgggggtcc ggatttcgtc ctctcaacct gg
//
LOCUS       DASTDG010000233         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_225101, whole genome shotgun sequence.
ACCESSION   DASTDG010000233 DASTDG010000000
VERSION     DASTDG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225101"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFO30_07760"
     CDS             <1..370
                     /locus_tag="VFO30_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419221.1"
                     /translation="FVLCHNPADPPFYTIDGTVVFSDVPATYWTLDSNTATQNAWGYQ
                     IIDGANHNVLLDNAASDNSAYDVEFVGESDRYGFHTPTSFKNFFIAGSHPNLIVKDCG
                     RGDVIKGNVQLVDHNSDPCF"
     gene            complement(463..2052)
                     /gene="guaA"
                     /locus_tag="VFO30_07765"
     CDS             complement(463..2052)
                     /gene="guaA"
                     /locus_tag="VFO30_07765"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9419222.1"
                     /translation="MNPPPAHSKILILDFGAQYTQVIARRIRELQVYSEVVRFDLPAA
                     KIKRLQPNGLILSGGPASVYDKDAPHPDPEIFSLGIPVLGICYGMQLMAHHLGGQVEF
                     SPRREYGPAILHVLDGSQLFAGLGAQLDIWNSHGDKLTALPKGFHAIARTENSPFAAI
                     ENLERNLFAVQFHPEVVHTPRGKEILQNFVYHICHCAMDWTMRSFIEEACERIRRQVG
                     DEKVVLGLSGGVDSSVTAALLHKAIGDQLTCIFVNNGLLRAREEEMVQRVFGENFHVQ
                     LKYVDASERFLTALKGVSDPEQKRKIIGNEFIAVFQHATEELLAEDRQNIARKHGGYR
                     FLAQGTLYPDVIESVSIGGNPASLIKSHHNVGGLPEKMHFELVEPVRQLFKDEVRQVG
                     LQLGLPKEIVYRQPFPGPGLAVRILGEVTPERLSILREADTIVQSEMEASDWYYRVWQ
                     SFAILLPVRSVGVMGDQRTYENTVVLRIVESQDGMTADWVRLPYELLARISSRISNEV
                     RGVNRVCYDISSKPPSTIEWE"
     gene            complement(2049..2264)
                     /locus_tag="VFO30_07770"
     CDS             complement(2049..2264)
                     /locus_tag="VFO30_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419223.1"
                     /translation="MKPCALFIALAFALAAGGCADPLERRSTEEVGSQMQRGITGQGQ
                     LIPSQSVNNPTGAPSAAETPPDYPSPR"
     gene            2397..>2947
                     /gene="aat"
                     /locus_tag="VFO30_07775"
     CDS             2397..>2947
                     /gene="aat"
                     /locus_tag="VFO30_07775"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9419224.1"
                     /translation="MIAPELLLQGYRLGVFPMAMEDDTIGWFSPDPRAIIPLQNFHVP
                     HALRRLVQKAAFETKIDNSFAEVIRACAKRKDTWINREIIESYERLHELGYAHSVETW
                     RKDKLAGGLYGVAIGGAFFGESMFHRERDASKVALVTLVEHLRANQFALLDTQWLTPH
                     LAQFGAIEVPRQHYLNLLKRAVDL"
BASE COUNT          632 a          845 c          825 g          645 t
ORIGIN      
        1 ctttgtcctt tgccacaacc ccgctgatcc accgttctat accatcgacg gcacggtcgt
       61 gttctccgac gttccggcca cgtattggac actggatagc aatactgcca cccagaatgc
      121 atggggctat cagattattg acggtgccaa tcacaacgtg ctgctcgaca atgctgcgtc
      181 agataactcg gcctacgatg ttgagtttgt cggcgagagc gatcgttacg gcttccacac
      241 gccgaccagc tttaagaact tctttatcgc cggatcacat cccaatttga tcgtgaaaga
      301 ttgcggccga ggcgatgtga tcaaaggcaa cgtgcaacta gtggatcaca actccgaccc
      361 ctgcttctaa agggaactat gtggcatcga gatcctgccg ataaacggct cggaagcgat
      421 ggcacactac ctatctggca atctgaaatc tgaaattcag aattattccc actcaatcgt
      481 gctcggcggt ttgctcgaga tgtcgtagca gacgcgattg acgccgcgga cttcgttgct
      541 gatgcgcgag gaaatgcgcg cgagcagttc gtacgggagc cgcacccagt cggcggtcat
      601 cccgtcctgg ctctcgacga ttcgcaagac cacagtgttt tcatacgtgc gctgatcgcc
      661 cattactccg accgaacgca ccggcagcaa gatggcgaac gattgccaga cgcgataata
      721 ccagtcgctc gcttccattt cgctctgcac gatggtatcg gcttcgcgta agatcgataa
      781 tcgctcgggg gtgacttcgc cgagaatacg cacggctagc ccgggcccgg gaaacggctg
      841 ccgatagacg atttccttcg gtaacccaag ctgcagtccg acttggcgaa cttcgtcttt
      901 gaacaactgc cgcaccggct caaccagctc gaaatgcatt ttctccggca aaccaccgac
      961 gttgtgatga ctcttgatca gcgatgccgg gttgccgcca atcgacacgc tctcaataac
     1021 gtcgggatac agcgttccct gggcgagaaa ccggtaacca ccgtgtttgc gcgcgatgtt
     1081 ttgtcgatct tccgcgagca actcctcagt cgcgtgttga aacacggcga tgaattcgtt
     1141 gccgatgatc ttgcgtttct gctccgggtc gctcacccct ttgagtgcag tgaggaaccg
     1201 ttcgctcgcg tcgacatatt tcagctggac gtggaagttt tcgccgaaca cacgctgcac
     1261 catttcctcc tcgcgtgcgc gcagcaggcc gttgttgacg aaaatgcagg taagttggtc
     1321 gccgatggct ttgtgcaaaa gcgccgccgt gaccgatgaa tcgacgccgc cgctcagccc
     1381 cagcacgact ttttcatcgc cgacttggcg gcggatgcgc tcgcatgctt cttcgatgaa
     1441 cgagcgcatc gtccagtcca tcgcgcagtg gcagatgtgg tagacgaaat tctgcaagat
     1501 ttctttgccc cggggtgtgt gcacgacttc gggatgaaat tggacggcaa ataaattgcg
     1561 ctccagattc tcgatcgcag caaagggtga attctcggtg cgggcaatag cgtgaaaacc
     1621 tttcggcagc gcggtgagtt tgtcgccgtg actgttccaa atgtccagct gtgcgccaag
     1681 gccggcgaac aattgcgagc catcgagcac atgcagaatc gcggggccat attcgcgccg
     1741 cggactgaac tcgacttgtc cgcccagatg atgcgccatc aactgcatgc cataacaaat
     1801 gccgagcacc ggaatgccta acgaaaagat ttctggatca ggatgcggcg catctttgtc
     1861 gtaaacgctg gctggtccgc cggagaggat cagaccgttt ggctgcaatc gtttgatttt
     1921 cgccgcagga agatcgaagc gcacgacctc cgaatagact tggagctcgc gaattcggcg
     1981 cgcaatgacc tgcgtgtact gcgcgccaaa atcgagaatt aagatcttgg aatgcgcagg
     2041 cggcgggttc atcgcggcga aggatagtct ggcggtgttt ccgcagctga tggcgcgccg
     2101 gtgggattat tgaccgactg gctcgggatc agctgaccct ggccggtaat gccgcgctgc
     2161 atctgtgagc cgacttcctc cgtgctgcgg cgttcaagcg gatcggcgca cccgccagcg
     2221 gcgagcgcga acgccagcgc gatgaacaac gcgcagggtt tcatctgctc atcttaggaa
     2281 tgaagtttgg aaaagcaaat tcgggcattg tagcggtgct cgtatcaagc accgaatgca
     2341 acaggcgctt ggcacaagca cctctacaac gtccgccgac tcgggtaacg tttaccatga
     2401 ttgcgccgga acttctgctg caaggctatc gcctcggcgt gtttcccatg gcgatggagg
     2461 acgataccat cggctggttc tcgcctgacc cgcgcgcaat tattccgttg cagaattttc
     2521 atgtgccgca cgcgttgcgc cggctcgttc agaaagcagc ctttgaaacg aagatcgaca
     2581 attcatttgc cgaagtgatt cgcgcttgcg cgaaacgcaa agacacctgg atcaaccgcg
     2641 agatcatcga aagctacgag cgtttgcacg agctcggata tgcgcactcg gtcgaaacct
     2701 ggaggaagga caagctcgcc ggtggtctgt atggtgtcgc tatcgggggt gcgttcttcg
     2761 gcgaatcgat gtttcaccgg gagcgcgacg cctccaaggt cgcgctcgtt acgttggtcg
     2821 agcatttgcg cgccaatcaa ttcgcactgc tagacacaca atggctcacg ccacatctgg
     2881 cgcagttcgg cgcgatcgag gttccacgcc agcattacct caacttgctc aaacgcgcgg
     2941 tcgacct
//
LOCUS       DASTDG010000234         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_486985, whole genome shotgun sequence.
ACCESSION   DASTDG010000234 DASTDG010000000
VERSION     DASTDG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486985"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFO30_07780"
     CDS             <1..1210
                     /locus_tag="VFO30_07780"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET9419225.1"
                     /translation="YVDDDCPPVPVDSEHPLYILYTSGSTGKPKGILHTTGGYLVGIY
                     TTTKYVFDIRDTDIFWCTADVGWVTGHSYVVYGPLANGATTLMYEGAPNWPEPDRLWK
                     IIEEYRVNILYTAPTAIRAFIRWGDEWVRERDLSSLRLLGTVGEPINPEAWMWYHEKI
                     GGGRCPIVDTWWQTETGAIMVTPLPGAIPTKPGSATLPFFGVDPAIVDSTGKEVGANV
                     GGKLIIRRPWPAMLRTIYGDKERYAKQYWTEFKGNYLTGDGARRDEDGYFWIVGRIDD
                     VLNVAGHRLGTSEIESALVSHANVAEAAVVGRTDELKGQAVVAFVTLKSGATPALSLK
                     QELRDHVGTHIGAIAKPDEVRFAEALPKTRSGKIMRRLLKEIASGKAVTGDTTTLEDF
                     SVLTKLSEEE"
     gene            1317..3188
                     /locus_tag="VFO30_07785"
     CDS             1317..3188
                     /locus_tag="VFO30_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419226.1"
                     /translation="MSFIRTGLRELALKVKRQRTRMALRHEKRLLQRSEIALGREGTA
                     QAANFPELRNEIVALKKLEQEQKEVALRIAQIEEGIKRIEGERQQNAEAQQEAIAKLE
                     AEKKPVVQRRNDAKGTADICERELKAVERRVEQNDETDRQLLKELTELQALTPPPPDL
                     DERLSSISARRSRLPDERAELVRARLGSEDACRLAKEKLIAAEAELSVAEKTIERARS
                     EFGARDRALAEKIRAQQQSLREARAQHQTVEERKNPAYLNIGRHLASQRIAPPNAAHL
                     LDDVHRHRAAVDRHLQHKEELALLSSQIDKQELRQFYFSLVSVLALLAIILPLVFQSP
                     KKREWLPQQTETILSINREQFERDDLPRRWRKEQPPEWTQVWRGLVSPATASPELNLP
                     RDIVRITRALTTEQAGKTREFILIEARGDVSRAIRSVEKDGTFKRRDIAGLPIWERPN
                     LAVARVGSNTLAVGRSEEVDELVRVRLGIDSDLKITGQLFDRFQALDRESALRLISRN
                     PPNLSHVFQPIFTSELLDAPQLLGLALTLQNPIKAKLLMKLSSAETAAQFANNLHKEP
                     QRWLHLQDSDLLLYVQPPEVIRQGTNLELRFIVPEHSARLLLRRIANLPSPGNVVAQ"
     gene            3185..3688
                     /locus_tag="VFO30_07790"
     CDS             3185..3688
                     /locus_tag="VFO30_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419227.1"
                     /translation="MTDVLLRGLRQPEYLHVLINPLPVYGLGAGLVALIVALFLRNRA
                     AHVTALVIILISSAAAFPVTRLGHQAYDRVLSMSDDAGRAWLDEHEERADDLVWFFYA
                     LALLCAAAILLPKKWPRLAVPLTITTLLLSIFCLGAGGYIAYAGGKIRHREFRNTPPP
                     PENTPVQ"
     gene            3705..5132
                     /locus_tag="VFO30_07795"
     CDS             3705..5132
                     /locus_tag="VFO30_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9419228.1"
                     /translation="MTTALDRAERDVEAHSASLQKPLGLFDLALTQILFVVGSGWVGT
                     AAKLGRSHLFFWLLAILLFYIPQAAVVIYLNSRMPLEGGIYQWAKLGLNEFAGFIAAW
                     NLWLLSITVIALGGMFVTTNLSYAIGASGAWMPNSKWCVSLISCALVAALGWAGARGL
                     SLGKWVHNVGAFAMLAVYAALVALPFVSLARGELPHYHPLALAAPTMSIFYCLNIFSK
                     LAVGGLSGFEYVAILAGEARAPARDIGRSVMIASPIIAVMFILGTSSVLAFIGDKPID
                     LIGPVPQTLRLGLRSFPVAGAIASFGIVLMTMRSISATSVHLTGSSRLPMVAGWDRLL
                     PAWFADLHPKYRTPINSILFVGLLTSAIAISSQIGAGIQEAFQLVDNAANVFYGIVYA
                     ILFVIPLAGATQIRSGAPLWLRVAALCGCTVSLLAIFFTVYPIIDVPSPLVFAAKIIA
                     VTLIANGLGVTIFMLGRRRQRNSTA"
     gene            complement(5146..5466)
                     /gene="cutA"
                     /locus_tag="VFO30_07800"
     CDS             complement(5146..5466)
                     /gene="cutA"
                     /locus_tag="VFO30_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET9419229.1"
                     /translation="MAEKALIVFCTIPDADTARRIAKEIVDLRLAACANILPHVHSIY
                     HWQGKTETADEALTIFKLAASSYPEFEAKLRSLHPYDVPEIIAYRIENGLPEYLRWVL
                     DSCT"
     gene            complement(5470..>6013)
                     /locus_tag="VFO30_07805"
     CDS             complement(5470..>6013)
                     /locus_tag="VFO30_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HET9419230.1"
                     /translation="PGSDYKTFLRSHLHESDFHRGEIYDVDGNFVGEHDGIELFTIGQ
                     RRGLPGGSLRPRYVVDLDAETNRVIVGDAEDLVCDEFEIDRVNWHSVAGVGDPGPRSA
                     TAATATLDVSVKIRYSHPGTQATVTDLENNRARIRLHAPQRAVTPGQAAVIYDGDVVI
                     GGGWICRAEPAWRKQALVSA"
BASE COUNT         1212 a         1772 c         1750 g         1279 t
ORIGIN      
        1 atatgtcgat gacgactgcc cgcccgtgcc agtcgacagc gaacatccgc tctacattct
       61 ttacaccagc ggttcgaccg gaaagccgaa gggcattctg cacacgaccg gcgggtatct
      121 tgtcgggatt tatacgacga ccaaatacgt gttcgatatc cgcgatacag acatcttctg
      181 gtgcacggct gacgtcggct gggtgacggg ccatagttat gtcgtttatg gtcccctggc
      241 gaacggcgct accacgttga tgtatgaagg tgcaccgaac tggcccgaac cagacaggtt
      301 gtggaagatt atcgaggaat atcgggttaa cattctctac accgctccaa cggccatccg
      361 cgcgtttatt cgttggggcg atgagtgggt acgcgaacgc gacctctcgt ccctgcgtct
      421 gctcggcaca gtaggcgagc cgattaatcc tgaggcctgg atgtggtatc acgaaaagat
      481 tggtgggggc cgctgcccaa tcgtcgatac atggtggcag accgaaaccg gcgcaatcat
      541 ggtcacacct ctgcccggcg cgatcccgac caagccaggt agcgccacac tcccgttttt
      601 tggcgtcgac cccgcgattg tcgattcaac cggcaaagaa gtgggcgcaa atgtcggtgg
      661 caaactgatc atccggcggc cgtggccggc gatgctccgc acgatctatg gggacaagga
      721 gcgctacgcg aaacaatact ggaccgagtt taaggggaat tacctcaccg gcgatggcgc
      781 ccgccgcgat gaagatgggt atttttggat cgttggccgc attgatgacg tgctcaacgt
      841 cgccggccat cgactcggca cgtccgagat tgaaagcgcg ctggtgagcc atgcaaatgt
      901 cgccgaagcc gcagtggtcg gccggacgga cgaactgaaa ggccaggccg tagttgcgtt
      961 tgtgacattg aaaagcggtg ccaccccggc tttatcactg aaacaagagt tgcgcgacca
     1021 cgtcggcacg cacatcggcg cgattgccaa gccggacgag gtgcgctttg ccgaggcgct
     1081 gccaaaaaca cgcagtggca aaatcatgcg ccggcttttg aaagagatcg ccagcggaaa
     1141 agctgtgacc ggcgatacca caaccctcga agatttcagc gtcctgacga aattgagtga
     1201 agaagagtga ttgatatgct tcgctcctgc tccgcatacc gctggattgc ctgttctgcg
     1261 tcgctcacgt ccccgcggct ccgttgtcga tcttgcgtcc gtcgtaaagt gagatcgtga
     1321 gtttcattcg cacaggtctg cgcgagttgg cgctcaaggt gaaacgccaa cgcacccgca
     1381 tggcattgcg gcacgagaaa cggttgctgc aaagaagcga gatcgcgctt ggacgcgagg
     1441 gcaccgcgca agcggcgaac tttccggagc tccggaacga aatcgtcgcg ctgaaaaaac
     1501 tggagcagga gcaaaaggaa gtcgcgctcc ggattgctca gattgaggag ggcatcaaga
     1561 gaatcgaagg cgagcggcag cagaatgcgg aggcgcagca ggaggcaatc gcgaagttag
     1621 aagcggaaaa aaagcccgtg gttcagcggc gcaacgacgc gaaaggcacg gccgatattt
     1681 gcgaacgcga gctcaaggca gtggaacgcc gcgtcgagca gaacgacgag actgaccgcc
     1741 aactgctcaa agagcttacc gaactgcaag cgctaactcc acccccgccc gacctggatg
     1801 agcgtttgtc gagtattagc gcgcgccgca gtcgtttacc ggatgaacgt gcagaattgg
     1861 tccgtgcccg acttggcagt gaggacgcat gtcggttggc gaaagaaaag ctcattgcgg
     1921 cggaagcgga gctttccgtt gccgagaaaa ccatcgagcg agctcgcagc gagttcggag
     1981 cgcgcgaccg cgcattggct gagaagatcc gcgcccagca acagtccctg cgcgaagccc
     2041 gcgcgcagca tcaaacggtc gaggaacgca aaaatcccgc ctacctcaac atcggacgcc
     2101 atctcgcctc ccagcggatt gctccgccga atgcggcaca tttgctcgat gacgtgcatc
     2161 gtcaccgcgc ggccgtagat cggcatttac agcataagga ggaactcgcg ctgctttcca
     2221 gccagatcga caagcaagaa ctgcgacaat tctatttcag tcttgtgtcg gtcctcgcgc
     2281 ttttggccat cattttgccg cttgtgtttc aatcgccgaa gaaacgcgag tggctcccgc
     2341 agcaaaccga aacgattctg tcgattaatc gcgagcagtt cgagcgcgac gatctgccgc
     2401 gccgctggcg gaaagagcag ccgcccgaat ggacgcaggt ctggcgcggt ctggtcagcc
     2461 ccgcaacggc gagtcccgaa ttgaacctgc cccgcgatat tgtgcgcatc acccgagcac
     2521 tcacgaccga gcaggccggc aagacgcggg agtttattct tatcgaagcg cgcggcgatg
     2581 tttcacgggc cattcgttcg gtcgagaaag atggcacatt caaacggcgc gatatcgcag
     2641 ggttgcccat ttgggagcga ccaaacctcg cggttgcgcg cgtcggctcg aacactctcg
     2701 ccgtcggccg gagcgaagaa gtggacgaac tcgtgcgggt gcggctcggg attgattccg
     2761 atctcaaaat caccggccag ttgttcgacc gttttcaggc gctcgatcgc gaaagcgcgt
     2821 tgcgtctgat ttcgcgtaat ccaccgaatc tctcgcacgt gttccaaccc attttcacca
     2881 gcgagctgct cgacgcgccg caattactcg gacttgcgtt gactctacaa aacccgatca
     2941 aggcgaagct gctcatgaaa ctcagctcag cggaaaccgc ggcgcaattc gcaaataacc
     3001 tgcacaaaga gccgcagcgc tggttacatt tgcaggattc tgacttgttg ctctacgtgc
     3061 aaccgcccga agtgatccgt caggggacga atcttgaatt gcgtttcatt gtgccggagc
     3121 acagcgcgcg cctgctcctc cgacgtatcg ccaatctgcc ttcgccagga aacgtcgtcg
     3181 cgcaatgaca gacgtcctcc tgcgcggtct gcggcagccg gaatatctcc atgtgctgat
     3241 caaccccctg cccgtttatg gcttgggcgc cgggctggtg gcattgatcg tggcgttgtt
     3301 tctgcggaat cgcgccgcgc atgtgactgc gctcgttatc atactgatca gttccgctgc
     3361 cgcattcccg gtcacccgtt tgggtcatca ggcctatgat cgcgttttat caatgagcga
     3421 cgatgcgggg cgcgcctggc tcgatgagca tgaagagcgc gccgatgatc tcgtttggtt
     3481 tttttatgcc ctggcgctgc tctgtgcagc cgcgattttg ttgccaaaaa aatggccgag
     3541 gttggccgta cctctgacga tcacaaccct gctattgtcg atcttctgtc tcggggctgg
     3601 tggatacatt gcctacgccg gaggtaaaat tcgccatcgt gaattccgaa acacgccgcc
     3661 accaccggaa aacacgccgg ttcaatgatt gatcagtctt actggtgacc acggcgctcg
     3721 atcgagccga acgcgatgtc gaggcgcata gcgcatcgct gcagaaaccg ctcggccttt
     3781 ttgatctcgc gttgacgcag attttgttcg tggtcggctc aggctgggtc ggtacggcgg
     3841 caaagcttgg gcgttcacat ctttttttct ggctgctggc gattttgctt ttctatatcc
     3901 cgcaagctgc ggtcgtcatt tatctgaaca gccgcatgcc gctcgaaggc ggcatctatc
     3961 aatgggccaa gcttggcttg aacgagttcg ccggttttat cgccgcctgg aacctgtggc
     4021 tgctttccat caccgtgatc gcacttggcg gcatgttcgt cacgaccaac ctttcctacg
     4081 ccatcggcgc aagcggagcg tggatgccga acagcaaatg gtgtgtgtcg ttgatcagtt
     4141 gcgccctggt ggccgcactc ggctgggctg gcgcgcgcgg gctctcgtta ggcaaatggg
     4201 tccacaacgt gggcgccttt gccatgctcg cagtctacgc cgccctcgtt gctttgccct
     4261 tcgtgagctt ggctcgtggc gaattgccgc actatcaccc gctggctctg gccgcgccga
     4321 ccatgtcgat cttttattgc ctgaacatct tcagcaaact cgcagtgggc gggctcagtg
     4381 gattcgaata cgtcgctatc ctcgctgggg aagcgcgcgc accggcacgc gatatcggcc
     4441 gctcggtcat gattgcgtcg ccaatcatcg ccgtgatgtt catccttggg accagctctg
     4501 tcctcgcgtt cataggcgac aagcccatcg atttgattgg ccctgtgccg caaactttgc
     4561 gcctcggatt acggtcgttt ccagtcgcgg gcgcgatcgc ttccttcggt atcgtcctca
     4621 tgaccatgcg gtccatctcc gcgaccagcg tgcatctcac gggcagctcg cgtctgccga
     4681 tggtggccgg ctgggatcgc ttgctgccgg cttggtttgc ggatttgcat ccgaaataca
     4741 ggacaccgat taactccatc ctgttcgtcg gtttgcttac gtcggcaatt gcgatttcga
     4801 gccagatcgg cgccggcatt caggaagcat tccagctcgt cgataacgcg gccaacgtct
     4861 tctacggcat cgtctacgcg attttgttcg tcattccgct cgccggcgcc actcagattc
     4921 gctccggtgc gccgttgtgg ctgcgcgtcg ccgcactttg tggctgcacg gtttcgttac
     4981 tcgccatttt cttcaccgtc tatccaatca tcgacgtccc gagcccgctg gtgttcgcgg
     5041 caaaaatcat cgctgtgacg ctgatcgcga acggcctcgg cgtgaccatc ttcatgctcg
     5101 gacgccgacg ccagcgcaac tcgactgctt aaagcagtca agttgttagg tacagctgtc
     5161 gagaacccag cgaagatatt ccgggagccc attctcgatc ctgtaggcaa tgatctcggg
     5221 gacgtcgtac ggatgcagcg accgcaattt tgcttcgaac tccggataac tgctggccgc
     5281 gagtttgaaa atcgtcagcg cttcgtcggc ggtttctgtc ttgccttgcc aatggtagat
     5341 cgaatgcacg tgcggcaaaa tgttcgcgca cgcggccagt cgaagatcga caatctcttt
     5401 tgcgattcgg cgcgcggtgt cggcgtcagg aattgtgcaa aagacgataa gcgctttctc
     5461 ggccattcgt taggctgaga caagagcttg cttgcgccac gccggctcgg cacggcagat
     5521 ccagccgccg ccaataacaa cgtcgccatc gtaaatgacc gcggcctgtc ctggcgtgac
     5581 cgcgcgctgc ggcgcgtgca aacgaatccg tgcccgattg ttctccaagt cggtcaccgt
     5641 cgcttgggtg cccggatggc tgtaacgaat cttcacgcta acatcgagcg tagctgtagc
     5701 cgcggtcgct gatcgcggcc cggggtcacc gaccccggct acagagtgcc agttcacgcg
     5761 atcgatttcg aactcatcgc aaacaaggtc ctccgcatcg ccgacgatga cgcggtttgt
     5821 ctccgcatcc agatcgacaa cgtagcgcgg ccggagtgat ccgccgggaa ggccgcgccg
     5881 ttgaccaatc gtgaacagtt cgatgccgtc gtgttcgccg acgaagttgc cgtccacatc
     5941 gtaaatctct ccgcgatgaa aatcactctc gtgcagatgg gagcgcagaa atgttttgta
     6001 atcactacca ggg
//
LOCUS       DASTDG010000235         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1127777, whole genome shotgun sequence.
ACCESSION   DASTDG010000235 DASTDG010000000
VERSION     DASTDG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127777"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFO30_07810"
     CDS             <1..214
                     /locus_tag="VFO30_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9419231.1"
                     /translation="AQLALAWVLAQGDDIVPIPGTKRRRYLQENIGALDVDLTSEDLE
                     RIEEVAPIGAFAGQRYPDWAMAMVNR"
     gene            complement(496..939)
                     /locus_tag="VFO30_07815"
     CDS             complement(496..939)
                     /locus_tag="VFO30_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9419232.1"
                     /translation="MSERYYDDLKVGDRFQSERLEVKEKELIEFAHKFDPQMFHLSRK
                     TAERTIFKGLIASGWHTAAMTMRLFVRTLNFAEGAIGLGVDELRWPNTVRPGDVLRVE
                     TEIVDLRPSRSKPSYGIVRLRNVTTNQRGEIVQSMLASAMVPRRA"
     gene            1053..1124
                     /locus_tag="VFO30_07820"
     tRNA            1053..1124
                     /locus_tag="VFO30_07820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Val,seq:tac)
     gene            complement(1115..1816)
                     /locus_tag="VFO30_07825"
     CDS             complement(1115..1816)
                     /locus_tag="VFO30_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9419233.1"
                     /translation="MKTLLLGLCLIAMTGAAEEKIRYVALGDSYTIGEGASPDESWPA
                     LLTRHLRAQDVDVDLVANPSVTGWTTQHLIDRELSIFRKEKPNFGTLLIGVNDWVQGV
                     NEKTFRQRFTFILDEMLKVLGNKNRLLVVTIPDFGVTPSGGQYARGRNISEGIASFNK
                     IVTNESLRRGLRVVDVFALSKKMGSDHSLVAADGLHPSAKEYAEWEKVIFPTALELLT
                     RSPAGNRKSKIDRGR"
     gene            complement(1821..2546)
                     /locus_tag="VFO30_07830"
     CDS             complement(1821..2546)
                     /locus_tag="VFO30_07830"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HET9419234.1"
                     /translation="MKTLGIIGGLGPESTVNYYQTIIRLYRERVGGDVYPQFFINNMD
                     LNKVREPVLANDLPRLANNIIAEIEKLARAGAEFGLIAANTPHLAFDEIAARSPIPLI
                     SIVEATCAVAKNRQFKRLAIFGTKFTMNGQFYARPFTRAGIDLVRPPAADQDYIHDKY
                     MNELVAGKFLPETKAGLLAIVDRMRAKFDPSSQSYGEAGIDAVILAGTELPLILREPQ
                     HNGIPFLNTTVIHCEAAVTKMFS"
     gene            complement(2586..3314)
                     /locus_tag="VFO30_07835"
     CDS             complement(2586..3314)
                     /locus_tag="VFO30_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9419235.1"
                     /translation="MPYNIDLHCHSLFSADGVSSPEEMIAGARAKGLHGIAITDHNTC
                     DGVTYLLDKGLMRLDGQPVDNFLILAGVEITTADGHLLCIGATLPYLKGKPALEVCEM
                     IHQRGALAIPPHPYDLFRAGIRFSTLEKLPVDAIEVFNAATTLRRYNRYAFKYAQLRG
                     LPMVAASDAHHAAAIGTAYTILNTDDFSTRGILAQIVKSNELNQEYLTPRDSVRKTWN
                     NWLRLRRRKKIPELVAKDRSDNGR"
BASE COUNT          734 a          989 c         1051 g          811 t
ORIGIN      
        1 cgcgcagctc gcactcgcgt gggtgctcgc gcaaggcgac gacatcgtgc cgattcccgg
       61 cacaaagcgc cgtagatatt tgcaggaaaa catcggcgcg ctcgatgtcg atcttacgag
      121 tgaagacttg gagagaatcg aggaggtcgc gcccattggc gccttcgccg gccaacgtta
      181 tcccgattgg gcgatggcca tggtgaatcg ctgaaagcga gacaaccgac tacgagacta
      241 cagacgactg accacctgac cagcgcatca gcccgatcac gaccaggctc gtcactcttg
      301 caactatttt gccgactttt ccggcttctt tctcgacggc tcggccgtaa ctgactggtc
      361 agaccgtaca tttcttgtct cggaaacgat cgagtgcatt cgtagattga aacggtcaaa
      421 tcatccgcga gtctccaagc ttcgatcttc gtatagtccc tcatgatgta gtccgtggtc
      481 ggtggtctcg ttgtcctacg cacgccgcgg caccatggcg gaggcgagca tgctctggac
      541 aatttcgccg cgctggttgg tggtcacgtt acgcaaacgc acaatgccgt aacttggttt
      601 tgagcgcgaa ggtctaagat caacgatttc tgtttcaacg cggagcacat cgccgggacg
      661 taccgtgttg ggccagcgca gttcatccac gcccaggccg attgcgcctt cggcaaagtt
      721 caacgttcgc acgaataacc gcatcgtcat cgccgccgtg tgccagccgc tcgcgatcaa
      781 tcctttgaag atcgtgcgct cggccgtctt gcggctgaga tgaaacatct gcggatcaaa
      841 tttgtgcgcg aactcgatca gctccttttc ctttacttcg agccgctccg attgaaaccg
      901 atcacccact ttcaaatcgt cgtagtaccg ctcgctcatt ttgaacctgc cgtgctcaat
      961 tcatgcgcga ttgcctcggc tagagcaacg tgtaaaatgt cgcccgaccc gctgatcact
     1021 tctcactgat cacttatcac ttcggtaaat gggggcggtt agctcagcgg tagagcatct
     1081 cgtttacacc gaggaggtcg ggggttcgaa cccctcaccg cccacgatca attttcgatt
     1141 tgcgattgcc ggctggcgat cgtgtgagca gctccagcgc ggtggggaaa atgactttct
     1201 cccattcggc gtattctttg gccgagggat gcagaccgtc cgcggcgaca agcgagtggt
     1261 cgctgcccat tttcttgctg agcgcgaaga catcgacgac gcgcagaccg cgtcgcagtg
     1321 attcgttggt gacgattttg ttaaacgagg cgatcccttc ggagatgttg cggccgcggg
     1381 cgtattggcc gccactcggg gtgacgccga aatcggggat cgtgacgacg aggaggcgat
     1441 ttttattgcc cagaaccttg agcatttcgt cgagaatgaa agtgaagcgc tgacgaaatg
     1501 ttttctcgtt aacgccttgg acccagtcat tcacgccgat gagtaacgtg ccgaagtttg
     1561 gtttttcctt ccgaaagatc gacagctcgc ggtcgatcag gtgctgcgtg gtccagccgg
     1621 tgaccgatgg gttggcgaca agatcgacat ctacgtcctg ggcccgcaag tgccgcgtca
     1681 gcaaggccgg ccacgattcg tcaggcgacg cgccctcgcc gatcgtgtag gaatcgccga
     1741 gcgcgacgta acgaattttc tcctcagcgg cgccagtcat tgcgatgaga catagcccaa
     1801 gcagcaatgt tttcatcagc tcacgaaaac attttggtca cagcggcttc gcagtggatg
     1861 accgtcgtat ttagaaacgg gatgccgttg tgctgcggct cgcgcaggat cagtggaagc
     1921 tcggtgccag caaggatgac tgcgtcgatg ccggcctcgc cgtagctctg cgaagacggg
     1981 tcgaacttag ctcgcattcg atcgacgatg gcgagcaggc cggctttggt ttcgggcaga
     2041 aactttcccg ccacaagttc gttcatgtat ttgtcgtgga tgtagtcttg gtcggcggcg
     2101 ggcggcctaa cgaggtcgat gcctgcacgc gtgaacggcc gagcgtagaa ctggccattc
     2161 atcgtgaatt tcgtgccaaa gatggcgagc cgcttgaatt gtcgattttt cgcaacggca
     2221 caggtcgctt ccacaatgct gataagcgga atgggtgaac gcgccgcgat ttcatcgaat
     2281 gccaaatgcg gcgtattggc ggcgattagt ccaaattcgg caccggcccg ggcgagcttt
     2341 tcgatttccg caatgatgtt gttcgccagc cgcggaagat cgttcgccag gaccggctcg
     2401 cgcactttgt tcaagtccat gttgttgatg aagaactgcg ggtaaacgtc gccgcctacg
     2461 cgttcgcgat aaagtcggat aatggtctgg tagtaattga ccgtgctctc cgggccgagg
     2521 ccgccgatga tgccgagcgt cttcaccaaa tcagcggtca gcggtcggaa gtcagcggtc
     2581 agaagtcacc ggccattgtc ggatctgtct ttcgcgacga gctcgggaat ttttttgcga
     2641 cggcggaggc ggagccaatt gttccaggtt ttacggacgc tgtcgcgcgg tgtgagatat
     2701 tcttggttca gttcgttgct tttgacgatc tgcgcgagaa tgccgcgcgt ggagaaatcg
     2761 tccgtgttca ggatcgtgta ggcggtgccg atggcggcgg cgtggtgcgc gtcgctcgct
     2821 gcgaccatgg gcaacccgcg gagctgcgca tatttgaacg cgtagcggtt gtaacggcgc
     2881 agcgtggtcg cagcgttgaa aacttcgatg gcgtcaaccg ggagtttctc aagcgtagaa
     2941 aagcgaatac cggcccggaa taaatcgtaa ggatgcggtg gaatggcgag cgcaccgcgc
     3001 tggtggatca tttcgcagac ctcgagtgcc ggttttcctt tcaagtatgg aagcgttgcg
     3061 cctatgcaaa gcaaatggcc atcggcggtg gttatctcca cgccagccag gatcaggaaa
     3121 ttatccaccg gctggccgtc gagccgcatc aagcccttat cgagcagata cgtgacgccg
     3181 tcacaggtgt tgtgatcggt aatggcaatg ccatgcagac ctttggcgcg cgcaccggcg
     3241 atcatttcct ccggactcga cacgccgtct gccgaaaaca aggaatggca atgcaggtcg
     3301 atgttgtagg gcattggcgc gagagtagct gaaccgtcgc aaaatcaaag cgcgtgattt
     3361 tgctctgtag ccaccgcgct attcgccgtt tcaatcggat gcctcacaga ggcgtggcta
     3421 caagctcaaa caagttgctc gctcaaggct tccgactcgg caggcgcttc tccggcagcg
     3481 tagttgaact gcatatcgta aagccgccgg taatagcccg acttttcgag caactcagcg
     3541 tgggtgccaa tttccctaat gacaccctca tccatcacga caatt
//
LOCUS       DASTDG010000236         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_976397, whole genome shotgun sequence.
ACCESSION   DASTDG010000236 DASTDG010000000
VERSION     DASTDG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976397"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFO30_07840"
     CDS             complement(<1..560)
                     /locus_tag="VFO30_07840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9419236.1"
                     /translation="MPQNETEKRIARLHAHGIVDLHFDLLLDLYETRKRPGALTAHYL
                     PEFEAGDVGVIGAAIYIEDRYLPELGLRVALDQIARVYAEVENAGERFAICKTFDEIT
                     RARAARKIALLITMEGVEPLAEDVDLLRAFYELGVRSVGLTHARRNAAGSGGIFAPSG
                     SPRDGLTPFGREIVRECERLGIIIDLA"
     gene            complement(570..1865)
                     /locus_tag="VFO30_07845"
     CDS             complement(570..1865)
                     /locus_tag="VFO30_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419237.1"
                     /translation="MTGFLLSPVTGHFSLLPMERSSLVLAVVLVLLGLVFLQQPRLRR
                     YDEVFLRWLLKNSVLPDKPAPLTIVEIGSPNSQTQAAEPQSREGFLQSGGAANSPLEA
                     ALFLQAALDFQPALVAFEPVLQWPEQAREQEEIFVDQAMRVPELLLAAELTATPEPDA
                     PVVDVAGFSHVTGKRGNLAAFSGVARQPSEDVRVLAKLGFINLPVEVADGIHVPLLFQ
                     YRGEVIPSFALEAVLLWLHVSQEEVTIDIGDAIQLPQGRRIPIESDGTVVINPNAANL
                     ARRRTSNELLLAAQQREQGKPLTERDNLSNQVLLARAASGGSGTRDALAAAIATMQTN
                     LFPRRVSWIFDCVSLALIGAFSGALRKIARVDLILIAIAFTAGYCLFALGILSHSSIW
                     LPGVLPLGALWVSILVCLVSRKPKPLAKTAAVIAPPPAP"
     gene            complement(1893..2021)
                     /locus_tag="VFO30_07850"
     CDS             complement(1893..2021)
                     /locus_tag="VFO30_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419238.1"
                     /translation="MKMNTLEKAYNALLHMEPEITLSEPLRRRAELPILRMLELSR"
BASE COUNT          425 a          636 c          620 g          377 t
ORIGIN      
        1 gcaaggtcga tgatgatgcc gagccgttcg cattcgcgca cgatctcgcg accgaatggc
       61 gtcagcccgt cacggggcga accgcttggc gcaaatatcc cgccgctgcc ggcggcattg
      121 cgacgggcat gcgtgagccc gacactgcgc acgccgagct cataaaacgc gcgcagaaga
      181 tcgacatctt cggctaacgg ctctacgcct tccatcgtga tgagcagcgc gatcttcctg
      241 gcggcgcgcg cgcgcgtgat ctcatcgaac gttttgcaaa tcgcgaaacg ctcgccggcg
      301 ttttcgactt cggcatagac tcgcgcgatc tggtcgagag cgacgcgcaa tccgagctcc
      361 ggcagatagc gatcctcgat gtaaatcgcg gcgccgatta cgccaacgtc accagcctcg
      421 aactccggca gataatgtgc ggtgagggca cccgggcgct tgcgcgtttc gtacaaatcc
      481 agcagcaaat cgaaatggag atcgacaatt ccatgcgcgt gcaaccgcgc gattcgtttc
      541 tccgtttcat tctgcggcat gtggcgcagt tacggcgcag gcggtggcgc gatcaccgcc
      601 gccgttttgg cgagcggctt tggtttgcgc gaaaccaggc aaactaaaat cgaaacccac
      661 aacgcgccga gtggaagtac gccgggcagc caaatcgatg agtgcgacaa aataccgagc
      721 gcaaacagac agtacccggc cgtgaacgcg atcgcgatga gaatgagatc gacgcgcgcg
      781 attttgcgga gggcgccgct gaacgcgccg ataagggcga ggctgacgca atcaaaaatc
      841 cagcttacgc gtcgcggaaa caaattggtt tgcatcgtag cgattgctgc ggccagggcg
      901 tcgcgtgtgc cgctgccccc gctggccgcg cgcgcgagca gcacttggtt cgacagattg
      961 tcgcgttccg tcagcggttt gccttgctcg cgctgttgcg ccgcaagcaa cagctcattg
     1021 gacgtgcgtc gccgcgcgag atttgccgcg ttcgggttga ttaccaccgt gccgtcggac
     1081 tcgatcggga tccttctgcc ctgcggaagc tgaatggcat cgccgatgtc aatcgtcact
     1141 tcttcctggc tgacgtgcag ccagagcaac actgcttcca gcgcaaacga tgggatcact
     1201 tcaccgcggt attggaaaag cagcggcacg tgaatgccat ccgccacttc aaccggcaga
     1261 ttgatgaagc cgagcttcgc gagcacgcgc acgtcctcgc tcggctgacg cgcgactccc
     1321 gaaaacgcgg cgagattgcc gcgcttgccg gtgacatgcg agaaaccggc aacgtctacg
     1381 acaggcgcgt ccggctcggg cgtggccgtc agctcggcgg cgagcagcag ctccggcacg
     1441 cgcatcgctt gatcgacaaa gatttcttcc tgctcgcgcg cttgctcggg ccactgcagc
     1501 accggttcaa acgccaccag cgccggctga aaatccagcg cggcttgcaa aaatagcgct
     1561 gcctccagcg gcgagtttgc ggcgccgccg ctttgcaaaa atccttccct ggactgaggc
     1621 tcggccgcct gcgtttgaga gttgggcgag cctatctcca caatagtcag cggcgccggt
     1681 ttatcaggca ggacagaatt cttgagcagc catcgcaaaa acacttcgtc gtatcggcgc
     1741 aaccgcggtt gttgcagaaa cacaagcccg agcagcacga gcacgactgc gagcaccagc
     1801 gaggatcgct ccatcggcag aagtgaaaag tggcctgtga cgggtgacaa gagaaaaccc
     1861 gtcacctgat ctcgttagtt gtcacggccg catcagcggc tcaattcgag catgcgaaga
     1921 atcggcagct ccgcgcgtct gcggagcggc tcggataacg tgatctccgg ctccatgtgc
     1981 aagagcgcat tgtaggcttt ctccagcgtg ttcattttca tgaaccggca ctcgccgcag
     2041 aagcaatgct cggtcggt
//
LOCUS       DASTDG010000237         9698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_995557, whole genome shotgun sequence.
ACCESSION   DASTDG010000237 DASTDG010000000
VERSION     DASTDG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995557"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="rimO"
                     /locus_tag="VFO30_07855"
     CDS             complement(<1..1209)
                     /gene="rimO"
                     /locus_tag="VFO30_07855"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9419239.1"
                     /translation="MSTRVGMISLGCAKNLVDAEIMLGSVLERGMEITSCAEEADVLV
                     VNTCAFIDSAKEESLAAILEAHQQRGLTKRANQKLIVSGCMSQRFARELRNEMPEIDA
                     FVGLDQVTELGAIVESVLADEDVDLAFANQRPRYIPDYDTPRFRLTPAHSAYVKIAEG
                     CNHPCSFCVIPQMRGRHRSRTMDSVLAEIRGLVAEGVREVNLISQDTTYYGMDLWPEK
                     AGPRQPVDSARGPTLAVLLRKIENIEGEFWVRLLYTHPAHWSDELIATIAQCKKVARY
                     IDIPLQHISESMLRRMRRETSRQHVEELIAKLRIGIPGVALRTTFVVGFPGETQEDFE
                     SLLGFIERTRFDRLGVFKYSREEGSRAATLPDQISARIKNARHRVAMSLQQKIAYELA
                     RERIGHKLKLL"
     gene            complement(1230..2198)
                     /locus_tag="VFO30_07860"
     CDS             complement(1230..2198)
                     /locus_tag="VFO30_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9419240.1"
                     /translation="MTIEGLGKRFQEARLARGLTLDEAARMTKIRPSRLAEIEAEDFS
                     QFPSLAYAKGFLQIYGKFLDVDVTPYLEAFETSGDVTVNGYSYLQENPPPAPVRTTVA
                     RRRPERASSSSPAPARRPSIAPLLIGIVVIVVGFWIGKLVVNLRRLAGPEPAKAAAAP
                     SASVASATPQFIGVPPPAQPAAPVTNAVANTPAPALAPPTRPSTTAVAAASPTATKQP
                     EVRRAEPVRAEDLQKAAAGTAPSPATEAAGANRVDIRPLKKTYLKVVVDNETEQPAFE
                     RWVSPSDGTVQFRGQHIAVRVLDRDAIQIKKNGKVLAEEDEDVTVE"
     gene            complement(2217..4631)
                     /locus_tag="VFO30_07865"
     CDS             complement(2217..4631)
                     /locus_tag="VFO30_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9419241.1"
                     /translation="MARPLKNSAWNEVFALVLLFLGTLLFLALISYTPKDLPAWIPWS
                     YLSPPNRPAQNFIGWFGAVLASICYFVVGAASYLIAAILLGFGSVKLFHPSLRVRKRV
                     PWIVLFLVSGACLLQLQTRHFLGWKNAFNIPGPGGWIGFFLGKRILLNSTGIVGSVLL
                     VTGVYIATLILMTGLRPIHVVRQTVAGTRRGLRKLHEWRLKRQLRKADAREQLAINQR
                     ELAKQRRKIERELKKKGAAVPEPMAPFSSPQELADRPAPKVVDAMALPNEPVAPRRKP
                     TLAELRVMPQRLTITPGLTSRTWEAKGYVPPGLDLLEEHDPEGRGGTDPAELQRVQQT
                     LIDTLAQFGIVVAPGDITRGPTITRYEVYPAKGVRVDKIVSLERDLARATRAERINIL
                     APIPGKDTVGIELANTRKVTVTLREILESADWEEARVRAKIPLALGKDVYGKAIIADL
                     AQMPHLLVAGTTGSGKSVCINGLVASMLFRFTPEELQFIMIDPKVVELQIYADLPHLR
                     YPVITDPKKVLLVLRGLIDEMEKRYKIFARVGVRNIIGFNARPAKKKDEGSASVSLAS
                     EQKEQAGGSLHSKDENEIPDKLPYIVVVIDELADLMQTAPADVESAIARITQMARAAG
                     IHLIVATQTPRADVITGVIKANIPSRIAFQVASKIDSRVILDENGADRLLGQGDMLYL
                     PPTASRLIRAQGVLVTDDEIRRIVEFVSAQSPPVFDAAIHEKLEAATTGSDDVTNEDE
                     ELVEKCLEIIRQEKRASTSLLQRRLRLGYTRAARIVDILEQRGILGPGEGAKPREILV
                     DLDAAV"
     gene            5051..6478
                     /locus_tag="VFO30_07870"
     CDS             5051..6478
                     /locus_tag="VFO30_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HET9419242.1"
                     /translation="MAKHKYLTSPPNITGMPPGVPFIIGNEAAERFSYYGMKSILTVF
                     MAHYILNQSGALAPMAPNEAYKYSHYFVTGVYFLPILGAIIADGWLGKYWTILSLSIV
                     YCFGHLTLAFMDSAWAIAVGQRTMIAIGLALICLGAGGIKPCVSANVGDQFGESNKHL
                     LSKMFGWFYFSINAGSFVSTLLCPWLLANPHYGPRWGFGIPGVAMFIATLFFWGGRKK
                     MVHVPPAGLGYLRETFSRQGLATIGRIAMVYVFILFFWALWGMSNGIEWTLQAEKMNL
                     HWLGMDLIAAQVQTANPILILLFIPLVNYVIYPAIDRVFPLTPLRKIGIGLFLTALSF
                     VVIVWIQGQIDAGLRPTVNWQFLAYIILTLGEAMVSITGLEFSYTQAPNSMKSSIMAL
                     WLLTVAAGEFFDARFNAWDLNADGTRKLTDYQYFSFFTILMFVASIVFVFVARYYKGR
                     TYLQSQQPTLDEIATEPILSSGAPT"
     gene            6511..7395
                     /locus_tag="VFO30_07875"
     CDS             6511..7395
                     /locus_tag="VFO30_07875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9419243.1"
                     /translation="MNKLKAIFIGADGLRHGWRFLLFAATIILLVQFFEKPAIDFAAT
                     KLHIDPNAFSAPAIIVSDGIDLILILIVIGLAALFERRRIDSYGLPVNQAFGGLFWNG
                     VLAALAMIAFVGAAILVTGGMRIQGVALRGIDVVTAPLLWLVAMLFVGLAEEYTFRGY
                     ALQSLWRGAGFWPAALITTAIFAGDHLEKPHENAIDIGMIFALGLVMCLSVRITGSLW
                     WAVGWHAAFDFGQLFIIGTPNGGRVPVDRLFDASFPGPAWITGGELGTEASYFMIPPT
                     IATFIYVLIFLRRGTPRS"
     gene            7438..8073
                     /locus_tag="VFO30_07880"
     CDS             7438..8073
                     /locus_tag="VFO30_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9419244.1"
                     /translation="MLNLATWLREKDEKWFQPFFARHPDIQVRNARTGEVNIDEADGL
                     LLSGGSDVAPEFLRQAVPDPSPIEDVDLVRDRWEFDVIPKTIARGLPIFAICRGMQVL
                     NVALGGTLKLDIPGHRAPEMKDNDVQPLRNDRAASHRFEKVNSSHHQALDLLGDGLVV
                     EAWCAADHIIEQVRLRDYPFTLGVQYHPERGKIYDALFEDFFARMTNSLQR"
     gene            8143..9021
                     /locus_tag="VFO30_07885"
     CDS             8143..9021
                     /locus_tag="VFO30_07885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9419245.1"
                     /translation="MTPQNAIAIVYDYDQTLSPSYMQDEVVFPVFGVDGAKFWQRCSE
                     LVREHGYDNELAYMKVLLDTLAMDRPTNAQLRSLGKKLNFYKGLPEMFEQFQNGLLTA
                     EHVAHGIMVEHYIISSGMKVLIEGSRLAPYVRAIFGCEFAEDEGGRITFPKRVISHTQ
                     KTQFLFRINKGLLDMSQDVNDHMDPDIRPIPFPNMIYIGDGPTDVPCFTVMKKNGGQA
                     IAVYNPDDPQRVGFKKCYQLSAHADRVKHIAPADYQPNTHLRMLLEQMVAETADRIVA
                     QRKQAIETGTIRAPKH"
     gene            complement(9216..>9698)
                     /gene="rph"
                     /locus_tag="VFO30_07890"
     CDS             complement(9216..>9698)
                     /gene="rph"
                     /locus_tag="VFO30_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9419246.1"
                     /translation="SAEIQRLIGRSLRAVVDLEKLGPRTMWVDCDVLQADGGTRTAAI
                     TGASLAVAIACRKLAREKKVEVPPIRKLIAAVSAGMLDGNPIVDLTYEEDKAVTVDFN
                     LVATEDGDFVEVQGSGEEATFSQAQLDELLVLARKGIAELIAAQRAVLARIMVSPPET
                     "
BASE COUNT         1881 a         2918 c         2753 g         2146 t
ORIGIN      
        1 aagcaacttg agtttatgtc caattctttc acgcgcgagc tcgtacgcga ttttttgctg
       61 gagcgacatc gccacgcgat ggcgcgcatt ttttattctg gcggaaattt ggtccggtag
      121 cgtcgcggcg cgggatccct cttcgcgcga gtatttgaat acgccgagcc gatcaaaccg
      181 cgtccgctcg ataaacccca ggagtgattc aaagtcctct tgcgtctcgc ctgggaagcc
      241 gacaacaaaa gtcgtgcgca gtgccacgcc cggaattccg atgcgtaatt tggcaattag
      301 ctcctcgacg tgttgccgcg acgtttcccg gcgcatccgg cggagcatcg actcgctgat
      361 gtgttgcaac ggaatgtcga tgtaacgcgc gacttttttg cattgcgcaa tcgtggcgat
      421 caactcgtcg ctccaatgcg ctggatgtgt gtagagcagt cgcacccaga actcgccttc
      481 aatattctcg atcttgcgca agagcacggc cagagtcggg ccgcgcgcgg aatcgaccgg
      541 ctgccgcggc ccggctttct ccggccacaa atccattccg taataagtcg tgtcctgact
      601 gatcaggttg acctcgcgca cgccttcagc gacaagccca cggatttcgg cgaggaccga
      661 gtccatggta cggctccgat gtctgccacg catttgcggg atgacacaaa aactgcacgg
      721 gtgattgcag ccttcggcaa tctttacata ggcggagtgc gccggcgtga ggcgaaaccg
      781 cggcgtatcg taatcgggaa tgtatctggg ccgctgattc gcgaaggcaa gatcgacatc
      841 ctcgtctgca agcacgctct cgacgattgc accaagctcc gtcacttgat ccaatccgac
      901 gaacgcgtcg atttccggca tttcgttccg cagctcgcgc gcgaatcgct gcgacataca
      961 accgctcacg atcagttttt gattggcacg tttcgttagg ccacgctgct gatgagcctc
     1021 caggatcgct gcgagcgatt cctcttttgc cgagtcaatg aacgcgcaag tgttgactac
     1081 cagcacgtcc gcttcttccg cgcacgaggt gatctccatc ccgcgctcga gcacgctgcc
     1141 cagcatgatc tcggcatcga ccagattttt cgcgcagccg aggctgatca tgccgactct
     1201 ggtgctcatc gtgaaaaccg cgaatcgcgc tattcgaccg tgacgtcctc gtcctcttcg
     1261 gccaggactt tgccattctt tttgatttga atggcatctc gatccagcac gcgcacggca
     1321 atgtgttggc cgcgaaattg gacagtccca tcagacggtg agacccagcg ctcgaacgcc
     1381 ggttgctctg tctcgttatc gaccaccact ttgaggtaag ttttcttcag cggccgaata
     1441 tccacgcgat tcgcaccggc cgcttcggtt gctggcgatg gggctgtgcc tgcggcggct
     1501 ttttgcaaat cctcagcgcg cactggctca gcgcggcgaa cctccggttg tttggtagcg
     1561 gtgggcgaag ccgcggcaac cgctgttgtg ctcggccgag tcggtggcgc gagcgcaggc
     1621 gcgggcgtgt ttgccaccgc gttcgtgact ggtgccgccg gttgggcggg cggaggcaca
     1681 ccaatgaact gcggcgtagc agaagcaacc gaagctgaag gtgccgccgc tgctttggcc
     1741 ggctcaggcc ccgccaggcg ccgcaagttg acaacgagtt ttccgatcca aaagcccacg
     1801 acaatcacaa caatgccaat gagaagcggc gcgattgatg ggcgtctggc cggcgcgggc
     1861 gatgaggagg aggcgcgctc tggccggcgc cgcgcgaccg tcgtccgtac cggtgccggc
     1921 ggcggatttt cctgcaaata agagtagcca ttcactgtca cgtcgccgga ggtttcgaac
     1981 gcttcgagat acggcgtcac gtcaacatcg agaaacttgc cgtagatctg cagaaagcct
     2041 ttcgcgtagg cgaggctcgg gaactgggaa aaatcttccg cctcgatctc ggccagccgg
     2101 gagggccgga ttttcgtcat ccgtgcggct tcatcgagtg tgagcccgcg cgccagccgt
     2161 gcttcctgaa accgtttacc gagcccttcg attgtcatcc tatttgtgcc ggcacgctag
     2221 accgcggcat caagatcgac gagtatctcg cgcggcttcg cgccttcgcc gggacctaga
     2281 ataccgcgct gctccagaat atcaacgatg cgcgccgcgc gcgtgtagcc gagccgcaaa
     2341 cgcctttgca gcagcgaagt ggaagcgcgc ttctcttgcc gaataatctc gaggcatttc
     2401 tcaactaatt cctcgtcttc gttcgtgacg tcgtcactac cagtggtcgc cgcttccagc
     2461 ttttcgtgaa ttgcggcgtc gaataccggc gggctttgcg ctgaaacgaa ctcgacgatg
     2521 cgcctgatct catcgtcagt tacgagcacg ccttgggcgc ggatgaggcg cgaagcagtc
     2581 ggcggcaggt agagcatgtc gccctgacct aacaaacggt cggcgccgtt ctcatccaga
     2641 atgacgcgac tgtcgatctt actcgctact tgaaatgcaa tccggctcgg gatgtttgct
     2701 ttgatcacgc cggtaatcac atcggcgcgg ggtgtctgcg tcgcaacaat aagatgaatc
     2761 ccggccgccc gcgccatttg cgtgattcgt gctattgcgc tctccacgtc cgccggcgcc
     2821 gtttgcatca aatccgccag ctcatcgatc acgaccacga tgtagggcaa cttgtcgggg
     2881 atttcgtttt cgtctttaga gtggagcgag cctccggctt gctctttttg ttcagacgca
     2941 agcgagacgc ttgcgctacc ttcatctttc tttttcgccg gccgcgcgtt aaagccaatg
     3001 atgttgcgga cgccgacgcg ggcgaaaatt ttgtagcgtt tctccatttc atcgatcaag
     3061 ccgcgcaaca cgagcagcac ttttttcggg tcagtgatga ctggataacg caggtgcggc
     3121 aggtcggcgt aaatctgcag ctcaacgacc ttcggatcga tcatgatgaa ctgcagttct
     3181 tccggcgtga agcggaagag catgctggcg acgagtccgt taatgcagac acttttgccg
     3241 ctgccagttg tcccggcgac gagcaggtgc ggcatttgcg caagatcggc gatgattgct
     3301 ttgccataca catccttgcc gagcgccaac ggaattttgg cgcgcaccct ggcttcttcc
     3361 caatcggcgg attcgagaat ttcgcgcaat gtcacggtga ctttacgcgt attcgcaagt
     3421 tcgatgccga cggtgtcctt gcctggaatg ggcgcgagga tgttgattcg ctcggcacgc
     3481 gtcgcgcgcg caaggtcgcg ttcgaggctg acgattttgt ccacgcggac gcccttggcc
     3541 gggtaaactt cgtagcgcgt gatggtgggg cctctagtga tgtcacctgg cgcgacgacg
     3601 attccaaatt gcgcgagtgt gtcgatcaac gtctgctgaa cgcgctgcaa ctcggctggg
     3661 tcggtgccac cgcgtccttc cggatcatgt tcctcaagca agtccagccc gggcggcacg
     3721 tatcccttcg cctcccaggt tcgactcgtt aggccgggag tgattgtcaa cctttgcggc
     3781 atgacccgca gctcggcgag cgtcggtttt cgccgtggcg cgaccggctc gttcggcagc
     3841 gccattgcgt cgacgacttt cggcgccggc cgatctgcca attcctgcgg cgagctgaat
     3901 ggcgccatcg gctccggcac ggccgcgccc tttttcttta gctccctttc gatcttgcgg
     3961 cgttgcttcg ccagctcgcg ctgattgatc gccagttgtt ctctcgcgtc agccttccgc
     4021 aactgccgtt tcagtcgcca ctcgtgcagc ttacggaggc cgcggcgcgt tccggcgact
     4081 gtttgcctca ccacgtgaat tggacgcaaa cccgtcatga gaatgagggt cgcgatgtac
     4141 actccggtaa ccagaaggac cgagccgacg atgcccgttg aattgagcag gattcttttg
     4201 ccaaggaaaa agccgatcca gccacccggc ccgggaatgt tgaacgcgtt cttccaaccg
     4261 agaaaatgcc gggtttgcaa ctgcagcaaa cacgcgccgg agacgaggaa cagaacaatc
     4321 cagggcacgc gtttcctcac gcgcaagctc gggtgaaaca gtttgaccga gccaaacccg
     4381 agcaggatgg ccgcaattag atatgaggcc gcgccgacga cgaaataaca gatgctggcc
     4441 agcaccgcgc cgaaccagcc aatgaaattc tgtgccggcc ggttcggcgg cgagagatag
     4501 ctccacggaa tccacgccgg caaatccttg ggcgtgtacg agatcaacgc taggaaaagc
     4561 aaagtgccga ggaagagcag aacgagagca aacacttcgt tccaagcgct gttcttgagg
     4621 ggacgtgcca ctttccggca agatagtagt cttgcgccag tagctttcaa ataactcctg
     4681 agttgtagag gcgcttgtgg caagcgccta tttccgccgg tttcagcgtt tgacatagac
     4741 gccgctgcac tctcgcgctt tggtcttgcg cggcgcggag tgactattac tgtcctgcgc
     4801 ctatgcgact tgctccgcgc aattgcgccg cagtttcggc gtgcgctttg ctcgttctga
     4861 tttgcgcgtg cgacaagcat cacgtcggcg agatgccgga agtgcagcgc gagggcctcc
     4921 agccgcccgc cgaatctgcc gaacccgcgg ctccgccgca tgaaggatcg acatcttctt
     4981 cctcctcacc gaagccgacg ccggtggatt tttttcgggc gactaaacct cgttaggcaa
     5041 cggccagttc atggctaaac acaaatattt gacctcgccg ccaaacatca ccgggatgcc
     5101 gccgggcgtg ccgttcatta tcggcaacga agcggcagag cgcttcagct actacggcat
     5161 gaagtcgatc ctgaccgtct tcatggcgca ttacatcctc aaccaatcgg gcgcgctcgc
     5221 gccgatggcg ccgaacgaag cttacaaata cagccattat tttgtgaccg gcgtctattt
     5281 cctgcccatc ctcggcgcga tcatcgccga cggctggctc ggcaaatact ggacaatcct
     5341 ttcgctctcc atcgtctact gcttcggtca cctcacgctc gcgttcatgg attcagcctg
     5401 ggcaattgcc gttgggcagc gaacgatgat tgccatcggc ctggcgctga tctgtctcgg
     5461 cgccggcggc atcaagccgt gcgtctccgc gaacgtcggc gaccagttcg gcgagtcgaa
     5521 caagcattta ctctcgaaga tgtttggctg gttctatttt tcgattaacg ccgggtcgtt
     5581 cgtgtcgaca ctgctctgtc catggttgct cgccaatccg cactacggcc cgcgctgggg
     5641 cttcggcatt cccggcgtcg ccatgttcat tgcgacactc ttcttctggg gcggccgcaa
     5701 aaaaatggtg catgtcccgc cggccggcct cggctatttg cgcgaaacat ttagccgtca
     5761 gggtctcgcg accatcggcc gtattgcaat ggtctatgtg tttattctct ttttctgggc
     5821 gctctggggc atgagcaatg gcatcgaatg gaccttgcag gcggagaaaa tgaacctgca
     5881 ctggttaggc atggacctga tcgccgcgca ggtgcagacg gccaatccga ttctgattct
     5941 gctcttcatc ccgctcgtta attacgtcat ctatccggcg atcgatcgcg ttttcccgct
     6001 cacacccctg cgcaagatcg gcatcggatt gtttctgacc gcgctgagct tcgtcgtcat
     6061 cgtttggatt cagggacaga tcgatgccgg tctcaggccg accgtgaatt ggcagttcct
     6121 cgcttatatt attctcacgt tgggtgaagc gatggtctcc atcaccggtc tggaattctc
     6181 ctacacccag gcgccgaatt ccatgaagag ctccatcatg gccctgtggc tgctcaccgt
     6241 tgcagcgggc gaatttttcg atgcgcggtt taacgcatgg gacctgaatg ccgacggcac
     6301 gcgcaagcta accgattacc aatacttctc cttcttcaca attctgatgt tcgttgcctc
     6361 gattgtgttc gtctttgtcg cacgctatta caaaggccgc acctatctgc aatcgcagca
     6421 gcccacactc gatgaaatcg cgaccgagcc gatcctctcc agcggcgcgc cgacttaatc
     6481 gcctaacgaa cgtgccgcgc ggcggagaag gtgaataagc ttaaagccat cttcatcggc
     6541 gcggacggac tccgtcacgg ctggcgattt cttctcttcg ccgccacgat tattcttctc
     6601 gttcaatttt tcgaaaagcc ggcgatcgat ttcgcggcca cgaagcttca tatcgatcca
     6661 aacgcattca gcgcgccggc cattatcgtg agcgacggga tcgatctgat tctgattctg
     6721 atcgtaatcg gtctggccgc gttattcgaa cgacgacgga tcgatagcta cggcctgcca
     6781 gtcaaccaag cattcggcgg cttgttttgg aatggagtcc ttgccgcgct ggcgatgatc
     6841 gcttttgtcg gcgcggccat actcgtcacc ggcggcatgc gcattcaggg tgtcgcattg
     6901 cgtggcatcg acgtggtgac agcgccgcta ttgtggctcg tcgcgatgct gttcgtcggc
     6961 ctcgctgagg aatacacctt ccgcggctac gccttgcaat ccttatggcg cggtgccggc
     7021 ttttggccgg cggcgttgat cacgaccgcg atttttgccg gggaccatct cgagaagccg
     7081 cacgaaaacg ccatcgatat cggaatgatt ttcgcacttg gcctggtcat gtgtctcagc
     7141 gtccgcatca ccggctcgct ctggtgggcg gtcggctggc atgccgcctt cgattttggg
     7201 cagcttttca tcattggcac gccaaacggc gggcgcgtgc cggttgaccg actctttgat
     7261 gcgagctttc ccggaccggc gtggattacc ggcggcgagc ttgggactga agcgagctat
     7321 ttcatgatcc ctccgacgat cgcgactttt atctacgttt taatctttct gcgccgagga
     7381 acgccccgtt cttagacaag cgcttggcac agccgcctct accacgcaga ttctgccatg
     7441 ctgaatctgg cgacatggtt gcgggaaaaa gacgaaaaat ggttccagcc atttttcgcc
     7501 aggcaccccg acattcaggt gcgcaacgcg cgcacgggcg aggtgaacat cgatgaagcc
     7561 gacggtctgc ttttgagcgg cggctccgac gttgcgccgg aatttctccg ccaggcagtt
     7621 cccgatccga gcccaatcga agatgtcgat cttgtgcgcg accgctggga attcgacgtg
     7681 attcctaaaa caatcgcgcg cggtctgccg atcttcgcca tctgtcgcgg catgcaggtg
     7741 ctcaacgtcg cgcttggcgg cacgctcaag ctagatattc ccggtcatcg cgcgccggag
     7801 atgaaagaca acgacgtgca gccgctgcga aacgatcgtg ccgcgtcgca ccggttcgaa
     7861 aaggtgaaca gctcgcacca ccaagcgctc gatttgttag gtgatggcct tgtcgtcgaa
     7921 gcctggtgcg cagccgatca tattatcgag caagtccggc tgcgcgacta tccgttcact
     7981 ctcggagtgc agtatcatcc cgagcgcggc aagatttacg acgcgctctt cgaggatttt
     8041 ttcgcgcgga tgacaaactc gttgcagcgc tgaagagatg aagcgttgaa gcggcgttgt
     8101 ttccgcttta actcttcaac gctttatcgc ttcatcgctg ccgtgacccc acagaacgcc
     8161 atcgccatcg tctacgacta cgaccagacg ctgagtccca gctacatgca ggacgaggtc
     8221 gtgtttccag tgttcggggt tgacggcgcg aagttctggc aacgctgcag cgaacttgtg
     8281 cgcgagcacg gctacgacaa cgagctcgct tacatgaaag tgttgctcga caccctcgcc
     8341 atggaccgac cgaccaacgc gcagctgcgt tcgttaggca agaaattgaa cttctacaaa
     8401 ggcttgcccg aaatgttcga gcaattccag aacggcttgc tcacggccga gcacgtcgcg
     8461 cacggcatca tggtcgagca ttacatcatc tcgtctggga tgaaggtgct cattgaaggc
     8521 agccggctcg ccccttacgt gcgcgcaatt tttgggtgcg agttcgccga ggacgaggga
     8581 ggccggatca cgtttccgaa gcgcgtcatc agtcacacgc aaaagacaca gttccttttt
     8641 cggatcaaca aaggcctgct cgacatgtcg caggatgtga atgaccacat ggaccccgat
     8701 attcgcccga tcccgttccc gaacatgatc tacatcggcg acggcccgac cgatgtgccc
     8761 tgtttcacgg taatgaagaa aaacggcggc caagccatcg ccgtttacaa ccccgacgat
     8821 ccgcagcgcg tcggcttcaa gaaatgttac cagctctccg cgcacgccga ccgcgtcaaa
     8881 cacatcgcgc ccgccgatta ccagccgaac acacatctcc gcatgttgct cgagcaaatg
     8941 gtggcagaaa ccgccgaccg catcgtcgcc caacgcaagc aagccatcga gaccggcaca
     9001 attcgcgcgc cgaaacattg agtcagtcgg gcactgcgac agcagctttt gctaacggca
     9061 acatctgcga gatcactgtt gagcacgcac agaagcgagt agaaacattc cacacgtctc
     9121 gaacaagtcg ccgtttagct cgggtgatgt atgagccaag gcgccgtgcg ccgtcctgca
     9181 atggcaggat tttctaactg gtcggattaa ccggtctacg tttccggcgg ggagaccatg
     9241 atgcgagcga ggacggcgcg ttgcgcggcg attagttcag cgatgccttt gcgcgccagc
     9301 accaatagct catcgagttg cgcctgagaa aacgttgctt cttcacctga gccctgcact
     9361 tcgacgaaat cgccgtcttc agtggcaacg agattgaagt caaccgtgac ggctttgtcc
     9421 tcttcgtagg taagatcgac aatcgggttg ccatccagca ttccggcgct cactgcagcg
     9481 atgagtttgc ggatcggcgg aacttccacc ttcttttctc gggcgagctt gcgacaggca
     9541 attgcgacgg caaggctcgc gccagtgatg gccgcggtgc gcgtcccgcc gtcggcttgg
     9601 aggacgtcac aatccaccca catcgtgcgc gggccgagtt tctcaagatc gacaaccgcg
     9661 cgcagcgatc gaccgatgag acgctggatt tccgcgct
//
LOCUS       DASTDG010000238         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_90623, whole genome shotgun sequence.
ACCESSION   DASTDG010000238 DASTDG010000000
VERSION     DASTDG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90623"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFO30_07895"
     CDS             complement(<1..281)
                     /locus_tag="VFO30_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9419247.1"
                     /translation="MSEALRVFVLADTHNKLPPVIIDLAKGADEIWHLGDVCAEPILD
                     ELRAIGQPITLVRGNCDPTNEWPLVADLTRDRFKFRLQHIPPARPPTAV"
     gene            407..1624
                     /locus_tag="VFO30_07900"
     CDS             407..1624
                     /locus_tag="VFO30_07900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9419248.1"
                     /translation="MSFNNFGLSLEVVRGTQAMGFTEPTPIQLRAFPIILAGKDLIGT
                     AQTGTGKTAAFALPILTLLAQHGKFRCLVLEPTRELAAQVETAFRDYGRFTDIRATVI
                     HGGVGYGKQKDDLKRGMDVVIATPGRLLDHLEQGTLHFRDVNILVLDEVDRMLDMGFL
                     PDVRRIVEKISTDRQTLLFSATLPPEIERLAAWVLRDPELVEIGVRRSPAETVTHAVY
                     PVAAEQKFDLLIALLERTNFDSVLIFCRTKQNADRVAHSLKAAQHSVAVLHSNRTQRE
                     RIEALEGFKSGKYEVMVATDIASRGLDIAGVSHVINYDVPEHPEDYVHRIGRTGRAQN
                     VGDAFTLMNGEEVGALQAIEHFIGQKIPRLKLENFSYLYTALFEPESAAGGRRAIGGG
                     RSHRGFSYGRRRR"
     gene            complement(1722..>2458)
                     /locus_tag="VFO30_07905"
     CDS             complement(1722..>2458)
                     /locus_tag="VFO30_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9419249.1"
                     /translation="TGLVRADQTIAEVQQALKEQGFYYGEISGEKTADTTAAIRRYQI
                     RNGLQVTGELNDETVKSLRSVPTTSSPPPVTTATAAPANTPQRREQTTEETEPSAPAP
                     PQPYGAPSQDRSATGPIHGDQFYPPGYPPGAPNAPIGPPPRGELFADTPYATAPPDVQ
                     RDIVIAAERALAGQGFYHGDIDGVYGPALEFSLRAYQSRVRLAVTGRLDLETLAALEL
                     LPGAHTPVYRPRRRLIPPVRGEWIRP"
BASE COUNT          485 a          740 c          733 g          500 t
ORIGIN      
        1 tccacggccg tcggcgggcg cgccggtgga atatgttgca agcggaactt gaatcgatcg
       61 cgcgtaagat ccgcaaccaa cggccactcg ttagtcgggt cacaattccc gcgcacgagc
      121 gtgatcggct gtccaatcgc gcgcagttca tcaagaattg gttccgcaca aacatccccg
      181 agatgccaga tctcatcggc gcctttggca aggtcaataa tcaccggcgg caacttgtta
      241 tgcgtatcgg cgagcacgaa gacgcggagc gcctcactca tgcgtctctc aggaaagcag
      301 gaacgcatga aaagaaccat ggaattcggt ttctgcttgg ctgcgttctg gagagatatc
      361 ttcttaacgt tttttgcctc ctttgtctga gtcgttaggt tgcgccgtgt cattcaacaa
      421 ctttggactc tcgctcgaag tcgtgcgcgg cacgcaggcg atgggtttta ccgagccgac
      481 gcccatccag ttgcgcgcct tccccatcat tcttgccggc aaagacctga tcggcaccgc
      541 gcaaacgggc accggcaaga ccgccgcgtt cgccttgccg attctaactc tcctcgcgca
      601 gcacggaaaa ttccgctgtc tcgtgctcga gcccacgcgc gaactggcgg cacaggtcga
      661 gacggcattt cgcgactacg gccggttcac cgacattcgc gcgaccgtca ttcatggcgg
      721 agtcgggtac ggcaaacaaa aagatgattt gaaacgcggc atggacgtcg tcatcgcgac
      781 gcccggtcgc ctgcttgatc atctcgagca gggcacgctg cacttccgcg acgtgaacat
      841 tctcgtgctc gatgaagtcg atcggatgct ggacatgggt ttcctccccg acgtgcggcg
      901 aattgtcgaa aaaatctcca ctgatcgaca aacgcttttg ttttcggcga cgctgcctcc
      961 ggagatcgaa cgccttgccg cctgggtctt gcgcgatcct gaactggtcg agatcggcgt
     1021 gcgccgttct cccgcggaaa cagttacgca cgccgtttac ccggtcgcgg cggaacagaa
     1081 attcgacctg ctcatcgcgc tgctcgagcg aacaaacttc gacagcgtcc tgattttttg
     1141 ccgcacaaag caaaacgccg accgggtggc ccactcgttg aaggcggcgc agcattcggt
     1201 cgccgtcctg cattccaatc gcacgcagcg cgaacgcatt gaagcattgg aaggtttcaa
     1261 gagcggcaaa tacgaagtga tggtcgcgac tgatattgct tcgcgcggac tcgatatcgc
     1321 cggcgtgagt cacgtcatca attacgacgt gcccgagcat ccggaggatt acgtccatcg
     1381 catcggccgc accggtcgcg cgcaaaacgt cggcgacgcg ttcacactca tgaacggcga
     1441 agaagtcggc gcgctgcagg cgatcgaaca tttcatcggc caaaagatcc cgcggttgaa
     1501 gctagagaac ttttcctatc tctacaccgc gctgttcgaa cccgaatctg ccgcaggcgg
     1561 acgccgtgcc atcggcggcg gccgatcgca tcgcggtttc tcttacggaa ggaggcgcag
     1621 gtaatcggaa gtcaggcgtc tcgcctgccg ttgtggtgta agatcatccg agagagcccc
     1681 gagcgggccg ggcgaggcgc gtgcattcga aaagcgagtt tctacggccg aatccactcg
     1741 ccgcgaactg gtggaatcaa acggcgacgc ggacgataaa cgggtgtgtg cgctccaggc
     1801 aacaattcca gcgcagcgag tgtttcaaga tcaagtcgtc ccgtgacagc gagacgaaca
     1861 cgggattgat aagcgcgcag cgaaaactcg agcgctgggc cataaacacc gtcaatgtcg
     1921 ccgtgataaa acccctgccc tgccagtgcg cgttccgccg cgatcacaat gtcgcgctgc
     1981 acatcgggcg gggcagttgc gtacggcgtg tccgcaaata actcgccgcg cggcggcggt
     2041 ccgatcggtg cattcggcgc gccgggcgga taccccggcg gatagaactg gtcgccgtga
     2101 attggtcccg tggcggatcg atcttgcgat ggcgcgccgt agggctgcgg cggtgcaggc
     2161 gcgctcggct cggtttcttc tgtcgtttgt tcgcgacgtt gcggtgtgtt ggccggcgcg
     2221 gccgtcgctg tcgtcacagg cggaggcgat gatgtggtcg gcacgctgcg cagcgattta
     2281 actgtttcgt cattcagctc gcccgttact tgcaaaccat tgcgaatctg ataacggcgg
     2341 atcgccgcgg tcgtgtcggc agttttttcg ccgctgatct cgccatagta aaacccctgc
     2401 tccttcaacg cctgctgcac ttcggcaatt gtttggtcgg cgcgaaccag gccagtgc
//
LOCUS       DASTDG010000239         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_194014, whole genome shotgun sequence.
ACCESSION   DASTDG010000239 DASTDG010000000
VERSION     DASTDG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194014"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1371)
                     /gene="kbl"
                     /locus_tag="VFO30_07910"
     CDS             complement(184..1371)
                     /gene="kbl"
                     /locus_tag="VFO30_07910"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HET9419250.1"
                     /translation="MLAQFEQEVSATLEEIKSQGLFKTERVITSPQDAHIRVADGREV
                     LNMCANNYLGLADHPMLIAAAKEALDTHGFGMASVRFICGTQDIHKQLEAALTEFLGT
                     EDTILYPSCFDANGGLFETLLTDKDAVISDELNHASIIDGIRLCKAQRFRYKHNDMAD
                     LEAKLKEASSARFRLIATDGCFSMDGTITNLADIVDLAGKNNALTMIDDAHATGFLGR
                     RGRGTHEYRNVMGKIDIITGTLGKALGGASGGFTSGRKEIVDLLRQRSRPYLFSNSLA
                     PHIVAATLKALEILSASTELRDKLEENTKYFRAALTERGLTIKPGTHPIVPIMIGDAA
                     KSQRFAARMLEKGIYVIGFFYPVVPHGTARVRTQVSAAHSRQDLEFAVKAFAETNEEL
                     KEA"
     gene            1482..2228
                     /locus_tag="VFO30_07915"
     CDS             1482..2228
                     /locus_tag="VFO30_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419251.1"
                     /translation="MPAPRISFVALLGAAALLLSACGTVSRTRALPPYEPPLAKTDFQ
                     NVRTTAYTHTESDHREFGNRNALGGELHAAGPAIHRAEYFPRAMAVSDTEDVDVRRVA
                     NSGTELQPFSLSETRTTRVTVTTRTTKTKRGVKRALAISRPPRIGSAAADWSRWPVGT
                     TFRLLSTGQIYRVEDYGWALSGRNTIDLYMANARDMNAWGARAQPIQILQWGNADESL
                     RLLEPHQSYPHIKRMVLELRGRDAEAAALQ"
     gene            complement(2252..3157)
                     /locus_tag="VFO30_07920"
     CDS             complement(2252..3157)
                     /locus_tag="VFO30_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419252.1"
                     /translation="MLSDLLRLRRFFALRAKKKKKQRAFGMTTWHEQDWLRTYAARTY
                     QGRGVIVDLGCFLGATTISLAEGLALNPYRKEQQIHAYDLFRWSKGFELWAQGKGIEG
                     RFADGESFLPEFLKRTERWRDYIIVHEQDLTQGQWQNGAIEFLLIDAMKTAEITSAIL
                     RAFFPDLLPGIGHVAHQDFAHCYTPWLHLAVYRLRDHFSVAADVPASGTTVFRCDKPI
                     SSELLNMDVSFARCDVKEIEAAFDYSFGLVPDEKKGNIIGAKAMAYRERGDIAHARKI
                     LAATPDDQPSLASELDQIKHLLGRG"
     gene            complement(3164..4210)
                     /gene="tdh"
                     /locus_tag="VFO30_07925"
     CDS             complement(3164..4210)
                     /gene="tdh"
                     /locus_tag="VFO30_07925"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HET9419253.1"
                     /translation="MQETMPAVVKSRAEPGLWLEEVPIPEVGGDDVLIRVLKTSICGT
                     DVHIYNWDAWAQKTIPVPMTIGHEFVGIVDLVGGHVKGFAKGDLVVGEGHITCGHCRN
                     CLAGRRHLCPNTQGVGVNRPGAWAEFVAIPQSNCWHADPSIPLDVLSCFDPLGNAVHT
                     ALSFDLVGEDVLITGAGPIGCMAIPICKHAGARHVVITDVNPYRLELARKMAPNVFGV
                     DVRTDKVADAMQKLDMKEGFDVALEMSGSPKAFDDILPHMFHGGKVALLGIMPGSAAI
                     DWNLVVFNQLTIKGIYGREMYETWYKMTALIQSGLDISAVITHRFPYTQFKEAIELMK
                     SGNSGKIVLDWSSC"
     gene            4316..5269
                     /locus_tag="VFO30_07930"
     CDS             4316..5269
                     /locus_tag="VFO30_07930"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9419254.1"
                     /translation="MDKLLWRREDRQLMRAILEQVEPKIATSRSVFVLRFISTVVLWS
                     IALVIAFSGFEFAFYALICVFGLIALWEFYGMLDHRGLPNFKVTALICGAVMLVGSFY
                     YFSKVGSAQSYDFEVAVLLFFLLTVFGRQMFQRLRHDEPLQTMAYTLFGLLYVLWLFN
                     FTTKILYLTPRSSAGAVTGQFYVLYLIAVTKFSDMGAYLTGSAIGRHQMVPHISAKKT
                     WEGFFGALGFALFCSLILFKLMPGHLSMLNWTHATVLGLLLGFAAVIGDLAESIIKRS
                     TGVKDSGNFLPGIGGALDLVDSLLFTAPLLFFYLRLVIRVP"
     gene            5300..5728
                     /locus_tag="VFO30_07935"
     CDS             5300..5728
                     /locus_tag="VFO30_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9419255.1"
                     /translation="MNRIEKAPEAYDLCVQIIGLAMKVHSTLGPGFLESVYQNALIWE
                     LRKSGLKSEAQKPISVQYDGQIVGAFAADLLVNDSVIVEIKAIQALAKAHEIQLVNYL
                     TATGLEERLLLNFGAERLQFKKKFRSAKRESASSESEAIL"
     gene            5754..6914
                     /locus_tag="VFO30_07940"
     CDS             5754..6914
                     /locus_tag="VFO30_07940"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9419256.1"
                     /translation="MKRKRVVVLGATGSIGESTLKVARDIPERMEIVALAANTNVEKL
                     ARAANDVRPEAVCLVDESKIDILQKELEYKPRIFAGEVGLREIACLTDAEMVLVAIVG
                     TAGLRPALAAIEAGKDLAVASKEILVMAGEIVMREARDNNVHVLPVDSEHNAIFQCLD
                     GRSSEIRRIILTASGGPFRATAAEDFVDLTPEQALKHPTWNMGLKITIDSATLFNKGL
                     EMIEAHWLFGVEMKQVEVVIHPQSIVHSLVEFADGSTLAQLSYSDMCFPIQYAITWPE
                     RVPNRLPPLDFSTLGKLEFELPRYEDFPALNLARRAGETAGTLPAVMNAANEVAVAAF
                     LDRRIGFPHIWQTVAAVMQAHTSVAHPNLDTILQADQWARAEGTRFVNSLKR"
     gene            6964..>7272
                     /locus_tag="VFO30_07945"
     CDS             6964..>7272
                     /locus_tag="VFO30_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9419257.1"
                     /translation="MLLHVARIIFVIFEVLVLFNLLIVVHELGHFLAARWRGLYIEKF
                     GIWFGKPLWKKTINGVQYSLGSLPFGGFVALPQLAPMDMIEGKADVDRTKLARISALD
                     K"
BASE COUNT         1473 a         1972 c         2171 g         1656 t
ORIGIN      
        1 gagacatact ccaaaccgtc tggcatcatc cgtccttttt cgtgcaatcg ctggtaaatc
       61 gcgggcgcgt ctttgaatcg ctcggtcacc atgtaaagca tgaggagaaa ttaaaagggt
      121 tacaaaagtt acaacgttaa aacggatgac cctggtaact tttgtaacgt tgtattgtaa
      181 cttttaagct tctttgagtt cttcgttcgt ttcggcgaaa gctttgacgg cgaactcgag
      241 atcttggcgc gaatgcgcgg ccgagacttg ggtgcgcacg cgcgcggtgc catgcggcac
      301 gactggatag aaaaacccga tgacgtagat gcctttctcc agcatccgcg cggcgaaccg
      361 ctgtgacttc gcggcgtcgc cgatcatgat cggcacgatc ggatgcgtgc cgggtttgat
      421 ggttagaccg cgttcggtca gggcagcgcg gaaatatttt gtgttctctt ccagcttatc
      481 gcgcagctcg gtcgaggcgc tgagaatttc gagggctttg agcgtggcgg cgacgatgtg
      541 tggcgcgagg ctgttcgaga aaagatatgg ccgcgatcgt tgccgcagaa gatcgacaat
      601 ctctttgcgc ccgctggtga acccgccact ggcgccgccg agcgccttac cgagcgtgcc
      661 ggtgatgata tcgatcttgc ccatgacgtt gcgatactcg tgcgtgccgc ggcctcgtcg
      721 gccaaggaaa ccggtcgcgt gggcgtcgtc gatcattgtg agcgcgttgt ttttgccggc
      781 cagatcgaca atgtcggcaa ggttcgtgat cgtgccgtcc atcgaaaaac agccgtcggt
      841 cgcaatgagg cgaaaacgcg cgctgcttgc ttctttcagt ttcgcttcga gatcggccat
      901 gtcgttgtgc ttgtagcgaa agcgctgcgc tttgcagagc cggatgccgt cgatgatcga
      961 ggcatgatta agttcatcgc taatgaccgc gtctttatcg gtgagtagcg tttcaaacaa
     1021 accgccgttc gcgtcgaaac atgacgggta caggatcgtg tcttcagtgc cgagaaactc
     1081 ggtcagcgcg gcttcgagtt gcttgtgaat gtcctgggtg ccgcaaatga atcgcacact
     1141 ggccatgccg aacccgtgcg tgtcgagtgc ttctttcgcc gcggcgatca gcatcgggtg
     1201 atcggccagc ccgagataat tgtttgcgca catgttcagc acctcgcggc cgtcggcgac
     1261 gcggatgtgc gcgtcctgtg gcgaagtgat gacgcgctcg gttttgaaaa gaccttgcga
     1321 ttttatctcc tcgagtgtgg ctgaaacctc ctgttcgaat tgggcgagca tacgtgtgca
     1381 gagattacac ggatttgcgc gcgtgaagag ccgaaaccgc gcaggcgaaa taaatcttgc
     1441 gtgggtgtga ataattgtta ataactctcg cccgaatcgc cttgcccgcc ccccgcatca
     1501 gtttcgtcgc cttgttaggc gccgccgcgc ttttgctgag cgcgtgcggc actgttagta
     1561 gaacacgcgc gctgccgccg tacgaaccgc cactggcgaa gaccgatttc cagaatgtgc
     1621 gcacgaccgc ctacacgcac accgaatcgg atcatcgcga gttcggcaat cgcaacgcgc
     1681 tcggcggcga attgcatgcg gcggggccgg caatccatcg agcggagtat ttcccccgcg
     1741 ctatggccgt ctccgatact gaagatgtcg atgttcgacg cgtcgcgaat tcgggtacgg
     1801 agctgcagcc gttttcgtta agcgagaccc gcaccacgcg agtgaccgtg acgacacgca
     1861 cgaccaaaac gaaacgcgga gtgaagcgcg ctcttgccat ttctcgaccg ccgcggattg
     1921 ggagcgccgc ggcggattgg tcgcgttggc cagtcgggac gacctttcgg ctattgtcga
     1981 ccggccagat ctaccgagtc gaggattacg ggtgggcatt gtccggccgg aacacgatcg
     2041 atctctacat ggcgaatgcg cgcgacatga acgcgtgggg cgcgcgggcg cagcccattc
     2101 agattttgca gtgggggaac gccgacgaga gtctgcggct gcttgagccg catcaatcct
     2161 atccgcacat caagcgcatg gtgctcgagc tccggggccg cgatgccgaa gcggcggcat
     2221 tgcagtaaac agcgcaccgt tgcggtccgc gttagccgcg cccaagcagg tgcttgattt
     2281 gatccagctc gctggcgagt gacggctggt cgtctggggt tgctgcgaga atcttgcgtg
     2341 cgtgcgcgat gtcgccgcgc tcgcgatagg ccatcgcttt cgcgccgatg atgtttccct
     2401 tcttctcgtc gggcaccagc ccgaacgagt aatcgaacgc ggcctcgatc tcctttacat
     2461 cgcagcgagc aaacgacacg tccatgttga ggagctcgga tgagatcggc ttatcgcagc
     2521 ggaagaccgt cgtgcccgat gctggcacgt cggccgcgac cgagaaatga tcccgtaacc
     2581 gataaacggc caaatggagc caaggggtat agcaatgggc gaaatcctga tgggcgacgt
     2641 ggccaatccc gggaagcaga tccgggaaga aggcgcgcaa aatggcgctt gtgatttcgg
     2701 cggttttcat tgcgtcgatg agcagaaatt caatcgcccc gttttgccat tgcccctgcg
     2761 tgaggtcttg ctcgtgcacg atgatgtaat cgcgccagcg ctccgttcgt ttcaagaatt
     2821 cggggagaaa actttcgccg tcggcaaagc ggccttcgat tcctttgcct tgcgcccaca
     2881 actcgaaacc tttgctccac ctgaaaagat cgtaggcatg gatttgttgt tctttccggt
     2941 aaggattgag cgccaatccc tcagctaatg agatcgtagt ggcgccgaga aaacagccaa
     3001 gatcgacaat cactccgcgg ccctggtacg tacgcgctgc gtacgtgcgc agccaatctt
     3061 gttcgtgcca ggttgtcatt ccgaaggcgc gctgcttttt ctttttcttc gcccgcagcg
     3121 caaagaaccg gcgcagccgc agcagatcgg acaacacggg tgctcagcac gagctccagt
     3181 cgagcacgat tttgccggag ttaccggatt tcatcagctc gatcgcttct ttaaattggg
     3241 tgtaagggaa gcgatgggtg atgacggccg agatgtcgag gccgctttgg atgagggcgg
     3301 tcattttgta ccaggtttcg tacatttcgc gaccgtaaat tcctttgatg gtgagttgat
     3361 tgaagactac cagattccaa tcgatcgcgg cgctgccggg catgattccg agcagtgcga
     3421 ctttgccgcc gtgaaacatg tgcggcagaa tgtcgtcgaa agcttttggg ctgccggaca
     3481 tttcgagcgc gacgtcgaag ccttctttca tgtcgagctt ttgcatcgca tcggcgactt
     3541 tgtcggtgcg aacgtcgacc ccgaaaacat ttggggccat tttgcgggcg agctcgagcc
     3601 ggtatggatt gacgtcggtg atgacgacgt gacgcgcgcc ggcatgttta caaatcggaa
     3661 tggccatgca accaatcgga cccgcgccgg taatgagcac gtcttcgccg acgagatcga
     3721 acgagagcgc agtatgaacg gcgttgccta acggatcgaa gcaggagagg acatcgagcg
     3781 ggatcgacgg atcggcgtgc cagcagtttg actgcggtat cgcgacgaac tcggcccagg
     3841 cgccggggcg attgacgccg acaccttgcg tattcgggca aaggtgacgt cgaccggcca
     3901 gacaattgcg gcagtgtccg caggtgatgt gtccttcccc gacgacgaga tcacctttgg
     3961 caaagccttt cacgtggccg ccgacaagat cgacaatccc gacaaattcg tggccgatcg
     4021 tcatcggcac tgggatcgtt ttctgcgccc acgcgtccca gttgtatata tgtacgtccg
     4081 tcccgcagat ggacgttttg aggacgcgga tcaggacatc gtcaccgccg acctcgggaa
     4141 tcgggacctc ttcgagccac aagcccggct cggcgcgact cttcactacg gccggcatcg
     4201 tttcctgcac ggcagaatgt tatccgctgg ttgcgcagat tcagcagaaa agaatccgaa
     4261 gtctaatccg cggaaatgga ttcggctgtc gctcaccacg gcgtctgtgt aatctgtgga
     4321 caaacttctc tggcgtcggg aagatcgaca attgatgcgc gctatcctgg agcaggtcga
     4381 gccgaaaatc gccacatccc gctcggtttt tgttttgcgc ttcatcagca cggtcgtgct
     4441 gtggtcgatc gcgctggtaa tcgcgttttc cgggttcgaa ttcgcctttt acgcgctgat
     4501 ctgcgtcttt ggtttgatcg cactctggga attttacggc atgctcgatc atcgcggcct
     4561 gccgaacttc aaagtcacgg ctctgatctg cggcgcggtc atgctcgtgg gcagttttta
     4621 ttatttctcc aaggtgggtt cggcgcagtc ctacgacttc gaggtggcgg tgctgctctt
     4681 ttttctgctc accgtgttcg ggcgacagat gtttcagcgg ctgcggcacg acgagccgct
     4741 gcaaactatg gcttacacgc tcttcgggct gctctatgtg ctgtggcttt ttaatttcac
     4801 gacgaaaatt ctttacctca caccgcgttc gagcgccggc gcggtgaccg gacagtttta
     4861 cgtgttgtat ctgatcgcgg tgacgaagtt cagtgacatg ggcgcgtatc tgaccggcag
     4921 cgcgatcggc cgccaccaga tggtgccgca catcagcgcg aagaaaacgt gggaaggctt
     4981 tttcggtgcg ctcgggtttg cgctcttttg cagcttgatc ttgttcaaac tcatgcccgg
     5041 tcacttatcg atgctcaatt ggacgcatgc gaccgtgctc gggttgttgc ttgggtttgc
     5101 tgccgtcatc ggtgatctcg ccgagtccat tatcaaacgc agcaccggcg tgaaagattc
     5161 aggcaacttt ttgcccggca ttggcggcgc gctggatttg gtcgacagtc tgcttttcac
     5221 cgcgccgctg ctgttttttt atctgcgctt ggtaattcgc gttccgtaga catctgagga
     5281 ttctgacaga attaacaaaa tgaacagaat agaaaaggca ccggaagctt atgatttgtg
     5341 tgtccagatt atcggtctgg caatgaaggt gcattcgacg ctgggtcccg gttttctcga
     5401 gtccgtctat caaaatgcgc tgatctggga attgcggaaa agcgggctga aatccgaagc
     5461 acaaaagccg attagtgtgc agtatgatgg gcaaattgtc ggcgctttcg cggccgattt
     5521 gctcgtcaac gattcagtaa tcgttgagat taaagcgatt caggctctag caaaagcgca
     5581 cgagattcag cttgtaaatt atttgacggc gaccggactg gaggaacgct tgctcctgaa
     5641 tttcggcgct gagcgactgc agtttaagaa aaagttccgt tcggcgaaga gagagtcagc
     5701 ttcgtccgaa tccgaagcaa ttttgtagat tctgtgcatt ctgtcgaaaa gaaatgaaac
     5761 gtaaacgggt cgtcgtccta ggcgcgaccg gttcgattgg cgagagcacg ctgaaagtcg
     5821 cgcgggacat tccggagcgg atggagattg tcgctctggc cgcaaacaca aacgtggaga
     5881 agctggcgcg cgccgcgaat gacgtccgcc cagaggctgt ttgtctcgtc gacgaaagca
     5941 agatcgacat cctgcaaaag gagcttgagt acaaaccgcg gattttcgcc ggagaagtcg
     6001 gcttgcgcga gatcgcctgc ctaaccgatg ccgagatggt gctggtcgca atcgtcggca
     6061 cggccgggtt gcggccggcg ttagcggcga ttgaagcggg caaagatctc gccgtcgcca
     6121 gcaaggagat tctcgtcatg gcgggcgaga tcgtcatgcg cgaagcccgc gacaacaatg
     6181 tgcatgtgtt gccggtcgat tctgagcaca acgcgatctt tcaatgcctt gacgggagat
     6241 cgtcagagat tcgccggatc atcttgaccg cgtcgggagg accgtttcgc gccacggcag
     6301 cagaggactt tgtcgatctt acgcctgagc aggcgctgaa acatccgacc tggaacatgg
     6361 gcctgaagat cacgatcgat tccgcgaccc tgttcaacaa aggcctagag atgatcgaag
     6421 cgcactggct ctttggcgtg gagatgaaac aggtcgaagt ggtgattcac ccgcaaagca
     6481 ttgtccactc cctggtcgaa ttcgctgacg gctcgacact cgcgcagttg agctactcgg
     6541 acatgtgttt cccaattcaa tacgcgatta cctggccgga gcgcgtaccg aaccgcttgc
     6601 cgccactgga tttttcgacc ttaggcaaac tggaattcga attgccgcgt tacgaagatt
     6661 tcccggcgct aaatctggcg cggcgcgccg gagaaacggc cggcacgctg cccgcggtca
     6721 tgaacgcggc gaacgaagta gccgtcgcgg cattccttga tcgacgaata ggcttcccgc
     6781 acatctggca gacagtcgca gcagtcatgc aggcgcacac gagcgttgcg catcccaatc
     6841 tcgatacgat attacaggct gatcaatggg cgcgggcgga ggggacgaga ttcgttaact
     6901 cgttgaagcg ttaaatcgtc taatccgtct gtcacttcac gattcaacgt ttcaacggtc
     6961 cacatgctgc ttcacgtcgc gcgcatcatt ttcgtcatct ttgaggtgct ggttctgttc
     7021 aacttgctca tcgtcgtgca cgagcttggg cacttcctcg cggcgcgctg gcgcggactt
     7081 tacatcgaga agttcggtat ctggtttggt aaaccgctct ggaagaaaac gatcaacggt
     7141 gtgcaatatt cgttaggctc gctccccttc ggcggcttcg tggcgttgcc gcagctcgcg
     7201 ccgatggaca tgatcgaggg caaagccgat gtcgatcgca cgaagctggc gcggatttcg
     7261 gcgctcgaca ag
//
LOCUS       DASTDG010000240        14269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_811702, whole genome shotgun sequence.
ACCESSION   DASTDG010000240 DASTDG010000000
VERSION     DASTDG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811702"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFO30_07950"
     CDS             complement(<1..512)
                     /locus_tag="VFO30_07950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9419258.1"
                     /translation="MKLSILVAVLLSARLVAAEEKQASKGAMKTSDEVAVIKTSEGDM
                     VVEFWKDAAPNTIENFKKLARSSFYNGTIFHRIVKGFMIQGGDPNSKDPAKERSYGEG
                     GPGYKIKAEFNQHPHDRGVISMARETDPDSAGSQFFICLAPVHRLDHQYTTFGKLIKG
                     DDVLEKIGNTP"
     gene            complement(544..4737)
                     /gene="carB"
                     /locus_tag="VFO30_07955"
     CDS             complement(544..4737)
                     /gene="carB"
                     /locus_tag="VFO30_07955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9419259.1"
                     /translation="MPRNNELKKILLIGSGPIVIGQGCEFDYSGVQACKALREEGYKV
                     VLVNSNPATIMTDPEFADRTYIEPITPEVIEAIIERERPDALLPTMGGQTALNAAMAL
                     YRNGALARHGVKLIGANAQAIAKGEDRQLFKEAMLRIGLDVPHSGVAHSSADAQRVAE
                     EIGTFPLIIRPAFTLGGSGGGIAYNRDELEEIASRGLALSPVNEVLIEESLVGWKEFE
                     MEVMRDRMDNCVVVCSIENFDPMGVHTGDSITVAPVQTLTDKEYQMMRDAAFAVIREI
                     GVETGGSNIQFAVHPDNGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLD
                     EIKNDITRETPACFEPTIDYCVVKVPRFTFEKFPQADATLTTQMKSVGEAMAIGRTFK
                     EALQKALRSLEIKRFGLLGDGRDKDVDFETLRLKLAIPNAERIFYIAQAFQKGVSIDE
                     VFELTKIDKWFLRNIAELVAEAHKLRNIDFQSVCPAGLQPAESGSTAERISAGRTGHR
                     PMFRPFDERAAIQRTRRNLPHWEQDGATYFVTFRLADAVPAELAAKWRQELETWRHFH
                     PEPWDAATAAEYRKRFLQTREDWLDQGHGSCVLRDRKIAQIVADSLRYFNGQRYELDA
                     FVVMPNHVHVLVQPAAGHALQDIVRSWKTFTARQINRARGRSGHVWMQESFDRIVREW
                     ESLVRCREYIARNPEKAGLSPDELVLCNDEKLQREHAHLRNIDFQSVRPAGLQPAEWA
                     KRADNMSARRTADLQLSESETAERISAGRTGQRRMFLHAKKLGFSDRQLAIAFGLTDQ
                     EVRRTRKINDVVPTYRLVDTCAAEFEAYTPYYYSTYGDENERRESGKRKVMILGGGPN
                     RIGQGIEFDYCCVHAAFALRELGFETIMVNSNPETVSTDYDTSDKLYFEPLTLEDVLN
                     IYDQEKPEGVVVQFGGQTPLNLANGLKAAGVPILGTQPQSIETAEDRQLFAAMLDRLG
                     LRQTPSGSAVSTEQAVEIARKIGYPVLVRPSFVLGGRGMELVYNENDLRRYVAAALEV
                     SERKSGVPRDSADEPPAYQPRDRPARRRAAGSGETPNLRPILIDRFLEDAIEVDVDCI
                     SDGEVTVIGAIMEHIEQAGIHSGDSACVIPTFSLSAKVLDELSTATKAMADELNVRGL
                     MNVQFAVKGEDVYVLEVNPRASRTIPFVSKAIGVPLAKLAAKVMAGKTLRELGFTREV
                     VPKHFSVKEAVFPFLRYEGKDISLGPEMKSTGEVMGVDVDLGLAYAKSQMAAPPPLPT
                     GGNVFISVKDSDKHAVIPVAREFVELGFRIIATSGTADALSRAKLCVTKVFKIQEGRP
                     NVLDQIKNGDISFIINTPSGKIPRQHEIEIRNAALGAKIPIMTTVRAAQASANGIRSL
                     QRSKLRVCSLQEYHSCVAA"
     gene            complement(4797..6197)
                     /locus_tag="VFO30_07960"
     CDS             complement(4797..6197)
                     /locus_tag="VFO30_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9419260.1"
                     /translation="MKLIALLAVSLVLGASAFAERTLLIGGTVINPADSNIVENAVVS
                     INGDRIEGVTTRKKATISSGAQSIDCTGKFILPGYIDTHVHFFQSGDLFTRPDVVDLN
                     SIRPYKDEVAWIKSHLDDTFVRYLRSGITSVVDVGGPFWNFEVRKRANATAKAPRVVV
                     AGPLISSVSRPQLDLGDPPIVKIDNPEQARAFVRKLAEQKPDLVKIWYIVDQDHPVES
                     FRPIVRATIEESHVHKIRVAVHATELETARAAVEEGADVLVHSVIDKPVDDAFVRLLK
                     DRHTILCPTLVVFERYGRTFANKLNLTPEEKAWGNPEVIATLDATKIPPDKLPDRIKT
                     ALSEPAAALDRIKKIYEIALPNLKKLEDAGVTIAAGTDAGNIGTIHGPALFRELQLMK
                     EAGLTNMQILQCTTLNAAKLFGGETGAHLGRIDNGYFADLLILNSNPLTDIAHASDID
                     SVIKGGVIYTAKSILR"
     gene            complement(6194..7315)
                     /locus_tag="VFO30_07965"
     CDS             complement(6194..7315)
                     /locus_tag="VFO30_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9419261.1"
                     /translation="MSTPERDEIPLTYRAARRVVGLVLLLVIGWFIVRSLRTILLLFA
                     IVFLIAMVLNPIVVWLQRHRVPRGLGVALILLTLVALAGTLTIVAIPPLTAQVEGLIR
                     RAPDLWHGVRSHFETLARRYPAVAATLPQTDEIAGKIGAQAGAVANLLLRSTIGFVGG
                     LFTIVLALLLLIFVLGNPQPLVAAYLALAPDRYRTQAHRTLARLARQMTAWVRGVAIN
                     GVVTGFSIGTGMWLIGVQPALVFGVMSFLGEFLPNIGAFLVAFPILFVALSMGATKFW
                     LALAIVLFVYQVEVNLLVPVVLGKEMRLHPVNILFFTLAMGTLFGILGAMLAVPAAAL
                     VQIVLDEFYLHPRHLDRAEIEREAAILVEGKRRVSSIPT"
     gene            7446..7925
                     /locus_tag="VFO30_07970"
     CDS             7446..7925
                     /locus_tag="VFO30_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419262.1"
                     /translation="MQRQPLFIYINDHVAGSVAALELIEHLISSYDGQPLESFFVDLE
                     REIKSDQVVLKQLLAVAEQKPSFFRNVAGWISEKFARSKVKFAGQRMGGLGLLQALEI
                     LALGIRGKQLLWRALAVSDWLALQNVDLAHLEQRAMEQGERVEEKRLVAAREAFTVR"
     gene            complement(7944..8783)
                     /locus_tag="VFO30_07975"
     CDS             complement(7944..8783)
                     /locus_tag="VFO30_07975"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET9419263.1"
                     /translation="MKAARNRLCASGCHYVRFAWLIPLLLIASPALGASASWQSTLTT
                     QRGDYPNLRPLRAKYVFGWAGITAASADVHFTQPSSARFQLEAAGRTVGLARALWKLD
                     VRAHAVADAETLRPMEVNQTEDYRRKTVVTHLNFGGDGVRGTRTETKIAAAAESKARE
                     FKFANLFDLHSALLFLRSQPLRDRSVYRVVVYPATSAYLATITVVGREKVSVRAGTYN
                     AIKADLQLHRLGKNLELEPHRKFRRATAWISDDQNRILLRVEAQIFIGRVFAELQAVR
                     FET"
     gene            complement(8844..9467)
                     /locus_tag="VFO30_07980"
     CDS             complement(8844..9467)
                     /locus_tag="VFO30_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET9419264.1"
                     /translation="MAIAAAVASPLAALAQGGLKIGTIDMQRAFKEYSKTKEAEAKIN
                     DAKNAAKKEYDDRADGYKKALDEINNLNKQLDAPALSADAKSQKAKERDDKIANIKNM
                     EREITEFRQTRERQLQEQAMRMREGIVKEITDVVMDKVKANNMDLVFDRSGMSLNGVP
                     VLMYARENSDFTSDVIAELNKPGRATTTSVEGPATASSPSPKKATKP"
     gene            complement(9545..11920)
                     /gene="bamA"
                     /locus_tag="VFO30_07985"
     CDS             complement(9545..11920)
                     /gene="bamA"
                     /locus_tag="VFO30_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET9419265.1"
                     /translation="MRLIRRPLRSPAFSFSVAIAFVLFATSARTQNPVAETPPIIRSI
                     DVQYSGPATISKERILAQIRTRIGRPYSEEVVNEDIKNLYKTGAVLNVRIFAQPQADG
                     VKVIVAVQTRSVVREIVIDGAQAVKPRRIRKAIPVKINSMVKEEDLEKGREDIIDIYR
                     AKGFNEADVQFRVEPIEEKRGTARVVYTINEGVKGAVSRINFEGNQHFSDRTLRKQLK
                     TRGKTLFYYLDKSGRLDEAQFQQDLDSVREFYQNHGYIDVELKDVRRQRADGRMTITI
                     VVNEGTQYHVGKISISGEKVTTEEKIRALLKMKEGSVYSPKALHDDAKAMADAYGSGG
                     YVDLVITPQGTPGGPGVIDLHYQIEEGARSFIQRINVVGNTRTKDKVIRREVLVTPGD
                     IFNTVRVETSKKRLENLGYFSKVETYPEDTGVPGRKDLTIEVQEKRTGSLSFGGGFSS
                     IDKLVGFVELTQGNFDVTNWPVLTGAGQKFRIRVQVGTERKDALISFTEPWLFDQPLS
                     LTGQAFFNESNYLSSYYDQRDYGFMVELRKPLNPFIYSSLAYRLEDFDIYNVDPLASA
                     ALELEKGSTVKSEVLAGLVWDRRDNPFLTRSGQRIAVTPYVAGGFLGGDVQIYGWDVE
                     ASQYFHLWWDNILLFNVEAATVNTWGSGDRVPIFDRLFLGGANNLRGFQFRDVGPKDV
                     NDEPLGGKTLARATVEYTFPIVEKARGAIFYDTGFDNPDAYDFSVGTQTKPRRDPARP
                     PLEYNNLASDIGVGIRLDLPIGPLRLDYGIPIQRAGNNYGGQFNFSVGYQF"
     gene            complement(12058..13149)
                     /gene="mqnC"
                     /locus_tag="VFO30_07990"
     CDS             complement(12058..13149)
                     /gene="mqnC"
                     /locus_tag="VFO30_07990"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HET9419266.1"
                     /translation="MVAINREALTEKVLAGERISSDDALELYGLPLEELGALADERRR
                     QAKCRSYNGRGNAIVTYIVDRNINYTNVCNVYCKFCAFYRTEKDADHYVLSFEEIDRK
                     LDELTAAGGVQILMQGGHHPKLPFQWYVDLLRHIREKYPHINIHGFSPPEFQHFAETF
                     RMPLREVIAEFKNAGLGSIPGGGGEILVDRVRRKISPLKIDSRGWLEVMQVAHELGLK
                     SSATMMFGHVETIEDRIEHLQRIRDQQEKSAGFTAFICWTFQPQHTALKIDNATGVAE
                     YLRTQALSRIFLDNIENIQSSWVTQGPGIGQVALKYGANDFGSVMMEENVVSSAGTTF
                     RLNAQQIESLIRDAGYEPRRRNNWYELLN"
     gene            13276..>14269
                     /locus_tag="VFO30_07995"
     CDS             13276..>14269
                     /locus_tag="VFO30_07995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9419267.1"
                     /translation="MPKSVAIVGAGVSGLTCGVLFAERRYRTAIFAEQSGQETTSGAA
                     AALWFPYDCEPRTKVVAWALTTYRVLVGLASDPRSGVSMLQLRQYSRTGALAIPEWAH
                     SLGAHCLSLIPSARRAVAQRRREVEESLTFASIFKSGFAMKVPLMDTTIYLDYLTNRF
                     RARGGEIHAGVRFDGLDAIDPDFDIVINCAGIGARELLHDVDLEPHRGQVAVVPKINN
                     LSCAVVCDEEPLMYAIPRTNDCLFGGTNELSDNLAIDRDTTSRIVNECVRTLKIDKPR
                     VLAERVGLRPYRNSGVRLERDKLRDGRTIIHNYGHGGSGFTLSWGCAEEVFKIAD"
BASE COUNT         2865 a         4223 c         3996 g         3185 t
ORIGIN      
        1 ggcgtgttgc cgattttctc cagcacatcg tcgcctttga tcaatttgcc gaacgttgtg
       61 tattgatggt caagccgatg caccggcgcg agacagatga aaaattgtga cccggccgag
      121 tccggatccg tctcgcgcgc cattgaaata acgccgcggt cgtgcggatg ctggttgaat
      181 tcggctttga ttttatagcc gggaccgccc tcgccatagc tcctttcttt cgccggatcc
      241 tttgagtttg ggtcaccacc ctggatcatg aagcctttga cgatgcggtg gaaaatcgtg
      301 ccattgtaaa agctggagcg cgcgagtttc ttgaaattct caatcgtgtt tggcgcggcg
      361 tctttccaaa attcaaccac catgtcgcct tcgctggttt tgattaccgc gacctcatca
      421 gatgttttca tcgcaccttt acttgcctgt ttctcttcag ctgccacgag ccgggcgctc
      481 aacaacactg caactaagat cgacaatttc atgcttcatc agtgccatga agcgaggcgc
      541 gtgtcatgcc gcaacgcatg agtgatactc ctgcaaactg caaacgcgaa gtttgcttct
      601 ttgcagcgac cgaatgccgt tggcgctggc ctgcgccgcg cgcacagtcg tcatgattgg
      661 aattttcgcg ccaagcgccg cgttgcggat ttcgatttcg tgctggcgcg gaatcttgcc
      721 gctcggcgta ttgatgatga agctgatgtc gccgtttttt atttggtcga gcacgtttgg
      781 gcgtccctcc tgaattttga agactttggt cacacacaat ttggctcggc tcaatgcgtc
      841 agccgtgccg ctggtggcaa taatccggaa tccaagctcg acaaactcgc gtgccacggg
      901 aattacggcg tgcttgtcgc tatctttgac actgatgaag acgttaccgc ccgtcggcaa
      961 cggcggtggc gccgccatct gcgatttggc ataggccaga ccaagatcga catcgacgcc
     1021 cataacttcg ccggtcgatt tcatctccgg gcctaacgaa atgtctttgc cctcataacg
     1081 gagaaaaggg aaaaccgcct ctttcaccga aaaatgcttc ggcacgacct ctcttgtgaa
     1141 tcccagttcg cgcagcgttt taccagccat caccttcgcc gcgagctttg ccaggggaac
     1201 gccaatcgcc ttgctcacga atgggatcgt gcgcgaggcg cgcggattca cctcgagcac
     1261 ataaacgtct tcgcccttga ccgcgaactg cacattcatg agcccgcgca cgttcaactc
     1321 atccgccatt gcttttgtgg ccgttgaaag ttcgtccagc actttagccg aaagtgagaa
     1381 ggtcgggatc acacacgcgc tgtcgccgct gtgaatcccc gcctgctcga tgtgttccat
     1441 gatcgcgcca atcaccgtta cctcgccatc gctgatgcaa tcgacatcaa cttcgatcgc
     1501 gtcttcgaga aaccggtcga tcaagatagg tcggaggtta ggcgtctcgc ctgatccggc
     1561 cgcgcggcgt ctcgctgggc ggtctcgcgg ctgataagcg ggaggttcat cagccgagtc
     1621 acgcgggacg cctgacttcc gttcactcac ttccagcgcc gctgcaacat atcggcgtaa
     1681 gtcgttctca ttatacacca gctccatccc gcgaccgccc aggacgaaac tcggcctaac
     1741 gagtacggga tagccgatct ttctcgcgat ttcgactgct tgctcggtgc tcaccgccga
     1801 tccactcggt gtctggcgta gacccagccg atcaagcatc gcggcaaaca gttgtcgatc
     1861 ttccgccgtc tcgatgctct gcggctgcgt gcccaagatc ggcacaccgg cggccttcag
     1921 cccgtttgca agattaagtg gcgtttgtcc gccaaactgc acgaccacac cctccggctt
     1981 ctcctggtcg taaatgttga gcacgtcctc gagcgtgagc ggctcgaaat aaagcttgtc
     2041 gctcgtatcg taatcggtcg aaaccgtttc cggattcgag ttcaccatga tcgtctcgaa
     2101 gccgagctcg cgcagcgcga acgccgcatg cacgcagcag taatcgaact cgattccctg
     2161 cccaatgcgg ttcggcccgc cgccgagaat catcactttg cgcttgccgc tttcgcgccg
     2221 ctcgttctcg tcgccgtagg tcgagtagta gtatggagtg taggcctcaa actcggccgc
     2281 gcaggtatcg acgaggcgat aggtcggaac gacatcgtta atcttgcgcg tgcgccgaac
     2341 ctcttggtcc gttaggccaa acgcaatcgc caattgtcgg tccgaaaatc cgagcttctt
     2401 cgcgtgaagg aacatacgcc tctggcctgt gcgcccagcg gagattctct ccgctgtttc
     2461 ggattccgag agttgtaaat ccgctgtgcg ccgagcggac atgttgtccg ctctcttcgc
     2521 ccattcagcg ggttgtaaac ccgctgggcg cacagactgg aagtctatgt tccgaaggtg
     2581 ggcgtgctcg cgctgaagtt tctcgtcgtt gcataatacc aattcgtctg gactcagccc
     2641 tgccttttcg gggttgcgcg caatgtactc gcggcatcgg accagcgatt cccactcgcg
     2701 cacaatgcga tcgaaactct cctgcatcca cacatggccc gagcggccgc gcgctctatt
     2761 gatctggcgc gcagtgaagg ttttccacga ccgcacaatg tcctggagcg cgtgtccggc
     2821 ggcaggttgg accaacacgt gcacgtggtt gggcatgact acaaacgcat caagctcgta
     2881 gcgctgccca ttgaaataac gcaagctgtc ggccacgatc tgcgcgattt ttcggtcgcg
     2941 caaaacacag ctgccatggc cctgatcgag ccagtcctcg cgtgtttgta aaaatcgctt
     3001 ccgatattcg gccgcggtcg ccgcatccca cggctcaggg tgaaagtgtc gccacgtttc
     3061 caactcctgc cgccatttcg cggccaactc tgccggcacg gcatcggcca agcgaaatgt
     3121 gacgaaataa gtagcgccgt cctgctccca atgcggaagg tttcgccgcg tgcgctgaat
     3181 agcggcgcgt tcatcaaatg gccggaacat aggcctgtgg cctgtgcgcc cagcggagat
     3241 tctctcggct gtgctcccgg attcagcggg ttgcaaaccc gctgggcaca cagactggaa
     3301 gtctatgttc cgaagtttgt gcgcttcagc gacaagctcg gcgatatttc gcaagaacca
     3361 cttgtcgatc ttcgttagct cgaagacttc atcgatcgag acgcctttct ggaatgcttg
     3421 cgcgatatag aagattcgtt cggcgttcgg aatcgcgagc ttcaatcgga gtgtttcaaa
     3481 atcaacatct ttatcgcgac cgtcgcctag caagccaaag cgtttgatct cgagcgagcg
     3541 cagcgctttt tgcagcgctt ccttgaacgt ccgaccgatc gccatagctt cgccgaccga
     3601 tttcatctgc gtcgtcaacg tggcgtcggc ctgaggaaat ttttcgaagg tgaagcgcgg
     3661 cactttgacg acgcagtaat cgatcgtcgg ctcaaaacac gccggtgttt cgcgcgtgat
     3721 gtcgttcttg atttcgtcaa gcgtgtagcc gacggcgagc ttggccgcga tctttgcaat
     3781 cgggaaacca gttgccttcg aagcgagcgc gctcgaccgc gagacgcgcg gattcatttc
     3841 gatcacgacc attcggccgt tgtccggatg cacggcaaac tggatgttcg atccgccggt
     3901 ctcgacgccg atctcacgaa tgaccgcgaa ggcggcgtcg cgcatcatct gatattcttt
     3961 atccgtgagc gtttgcaccg gggcgacggt gatggagtcg ccggtgtgca cccccatggg
     4021 atcgaaattc tcgatcgagc agacgaccac gcagttgtcc atccggtcgc gcatcacttc
     4081 catctcgaat tctttccagc cgactaacga ttcttcgatg agcacttcgt tgacgggcga
     4141 gagcgcgagg ccgcgcgagg cgatctcctc cagctcgtcc cggttgtagg cgatgccgcc
     4201 gccgctgccg ccgagcgtga acgccggccg gatgatcaac ggaaatgtgc cgatctcttc
     4261 cgccacgcgc tgcgcgtccg ccgaggaatg cgccacgccc gagtgcggca cgtccagacc
     4321 gatgcgcaac atcgcttcct tgaataattg tcgatcttcg cccttcgcga tcgcctgcgc
     4381 gttcgcaccg atcagtttca cgccgtgtcg tgcgagcgcg ccgttgcgat ataaagccat
     4441 ggcggcattg agcgccgtct gaccgcccat tgtcggcaac aaggcgtccg gccgttcgcg
     4501 ttcgatgatc gcctcgatca cctcgggcgt gatcggctct atataagtac gatcggcgaa
     4561 ttccggatcg gtcatgatgg tggcggggtt cgagttgacc agcacgacct tgtagccttc
     4621 ctcccgcagt gccttgcacg cttgcacgcc ggaataatca aactcgcaac cctgcccgat
     4681 gacgatgggc ccggagccga tgaggagaat cttctttagc tcgttatttc gcggcatctg
     4741 cgcagcgcgg agaataacgc aaccgcgtcc aaattgcagg ccgtaatctc gtccagctaa
     4801 cggagaatag atttggccgt gtaaatcacc ccgcctttaa tgacgctgtc gatgtccgac
     4861 gcatgcgcga tgtccgtgag cggattcgaa ttgaggatca gaaggtcagc aaaatagcca
     4921 ttgtcgattc tgccaagatg cgcccccgtt tcaccgccga aaagtttggc cgcgttaagc
     4981 gtggtgcatt gcaggatttg catattcgtt aggccggctt ccttcatcag ctgcaactcg
     5041 cgaaacagtg ctggcccgtg gattgttccg atgttcccgg cgtcagtgcc cgcggcgatt
     5101 gtcacgccag cgtcttcgag cttcttcaaa ttcggcaggg cgatttcgta aattttcttg
     5161 atccggtcga gcgccgccgc cggctcggac aatgccgtct tgatgcggtc cggcaatttg
     5221 tctggcggga tttttgtcgc atccagcgtc gcgatcactt ccggattacc ccaagccttt
     5281 tcttccggcg tcagattcaa tttgttcgcg aaggtgcggc cgtagcgctc gaataccacg
     5341 agcgtcggac ataagattgt atgtcgatct ttcaacaatc ggacgaatgc gtcgtcgacc
     5401 ggtttatcga tcacactgtg gacgagcacg tcggcgcctt cctcaacggc ggcgcgcgcc
     5461 gtttccagtt cagtcgcatg gaccgctaca cggattttat ggacgtggct ttcttcaatc
     5521 gtcgcccgca cgatcgggcg aaagctttcg accggatggt cttggtcgac gatgtaccaa
     5581 atcttcacga ggtctggctt ttgctcagca agtttgcgca cgaacgcgcg cgcctgctcg
     5641 ggattgtcaa tcttgacaat cggtggatcg cccagatcga gctgcggccg cgacacgctg
     5701 gagatcaacg gcccggcgac tacgacgcgc ggcgccttcg ctgttgcgtt cgcgcgcttt
     5761 cgcacttcga aattccaaaa cggcccgccg acatcgacca cgctcgtgat gccgctgcgc
     5821 agataacgca cgaacgtgtc gtcaagatgc gacttaatcc acgcgacctc gtctttgtac
     5881 ggacggatgc tgtttagatc gacaacgtcc gggcgcgtga ataaatcgcc ggattgaaag
     5941 aagtggacgt gggtgtcgat gtagccgggc agaatgaatt tgcccgtgca atcgattgac
     6001 tgagcgccgc ttgagatcgt cgctttcttg cgcgtggtga cgccttcgat tctgtcgcca
     6061 ttgatcgaaa cgaccgcgtt ctcgacgatg ttactgtccg ccgggttaat gactgtgccg
     6121 ccgataagca atgtccgttc ggcgaaggca ctcgccccga gtacgagcga aaccgcgagg
     6181 agggcgatca gtttcacgtt ggaatactcg acacgcgacg tttgccctcc accaaaatcg
     6241 cagcctcgcg ctcgatctca gcgcgatcga gatggcgcgg gtgcagataa aattcgtcga
     6301 ggacaatttg aaccagtgcg gccgcaggca cggcgagcat tgcgccgagg attccaaaca
     6361 gagtgcccat cgccagggtg aagaacaaaa tgttgaccgg gtgcaggcgc atttctttgc
     6421 cgagcacgac cggcacaagc aagttgacct ccacctggta gacgaagagt acgatcgcaa
     6481 gcgcgagcca gaacttcgtg gcgcccatac tgagcgcgac aaagagaatt gggaacgcaa
     6541 cgagaaacgc gccaatgtta ggaagaaact cgccgagaaa cgacatcacg ccgaaaacga
     6601 gcgccggttg cacaccgatg agccacatgc ccgtaccgat ggagaaaccg gtgacgacgc
     6661 cgttaatcgc cacgccccgg acccaggccg tcatctgccg cgcaagccgt gccagggtac
     6721 ggtgggcttg cgtccggtac cggtccggcg cgagcgcgag atacgcagcg acgagtggct
     6781 gtggattgcc cagcacaaaa atgagcagca gtagggctaa cacgatggtg aacaggccgc
     6841 cgacaaaccc aatggtcgat cttaagagca ggttcgccac ggcaccggcc tgggcgccga
     6901 ttttgcccgc gatctcatcg gtctgcggca atgtcgccgc aactgcgggg tagcggcgcg
     6961 caagcgtttc gaagtgactg cgcacgccgt gccataggtc aggggcgcga cggatgagtc
     7021 cctcgacctg agcggtaagc ggcggaatgg caacgatggt cagcgttccc gcaagcgcaa
     7081 cgagcgtgag cagaatcaat gcaacgccga ggcctcgcgg aacacgatgg cgctgcagcc
     7141 aaacgacgat cggattcagc accatcgcga taagaaaaac aatcgcgaag agcagcagaa
     7201 ttgtgcgcag cgaacgcaca atgaaccagc cgatgacgag caaaaggacg agaccaacaa
     7261 cgcggcgtgc cgcgcggtag gtgagcggga tctcgtcgcg ctccggtgtg ctcatcgatt
     7321 gcttttgcgc tagaggctaa tacatcgctg ctggcgcgtg aattactttc ggctacttcg
     7381 ctctgcggca atcgcagagc gagaaggatg cagccacgtt ccgtgaactg tgcgaaccac
     7441 agtgaatgca acgccagccc cttttcattt atatcaacga ccacgtcgcc gggtcggtgg
     7501 cggcgctcga actgatcgag cacttgatca gctcttatga cggtcagccg ctcgaatcat
     7561 tctttgtcga tcttgagcgt gaaattaaat cggatcaggt ggtgttgaag cagctcctcg
     7621 ccgtagcgga gcaaaagccg agctttttcc gtaacgtggc tggttggatt agtgagaaat
     7681 tcgcccgctc gaaggtaaaa tttgccggcc aacggatggg cgggcttggt ctcctccaag
     7741 cattggaaat cctcgcgctc ggaatccgcg gcaagcaact gctctggcgt gctctggctg
     7801 tttcagactg gttagctttg caaaatgtcg atcttgcaca tctggaacaa cgagcgatgg
     7861 agcaagggga acgcgtggag gaaaaacggc tcgtcgcggc gcgcgaagcg ttcacggttc
     7921 gatgatgcgc tgaagcagcg atgttatgtc tcgaaccgga cggcctgtag ttcggcgaac
     7981 actctcccga taaatatctg cgcttcgacc cgcagcagga tgcggttctg gtcgtcggaa
     8041 atccatgcgg tcgcacgacg gaatttccga tgcggctcca gctcgaggtt tttaccaagg
     8101 cgatgaagct gcaaatccgc tttgatcgcg ttgtaagtgc cggcacgcac ggagactttt
     8161 tcgcggccga ctaccgtgat tgtggccagg taagccgacg tcgccggata cacgaccacg
     8221 cgataaacac tgcggtcgcg cagcggctgg ctccgcaaaa acaggagcgc ggagtggagg
     8281 tcaaacagat tcgcgaattt aaactcgcgc gctttgctct cggcggccgc ggcgattttg
     8341 gtttccgtgc gtgtgccgcg cacaccatcg ccgccgaagt tcagatgagt cacaaccgtt
     8401 ttgcgtcggt agtcttccgt ctgattgact tccatcggcc gcagtgtttc tgcgtcggca
     8461 acggcatgag ctctcacatc gagtttccaa agagcgcgcg ccaaaccaac tgtccggccg
     8521 gcggcttcga gttgaaaacg tgctgaggac ggctgggtga aatgcacgtc cgcgctcgcc
     8581 gcagtgatgc cggcccaacc aaaaacatat tttgcgcgca gcggacgcag gttgggataa
     8641 tcgccgcgct gtgttgttag cgtggactgc caggatgcag acgcgccaag cgccggtgag
     8701 gcgatcagga gtagcggaat gagccacgcg aagcgaacat aatggcagcc actcgcgcag
     8761 agcctgttgc gtgcagcttt caccggtacg gaaaaaacgc acgcgagcct ttgcccgcgt
     8821 gcgttcgaaa aatgtcgatg ttgttaaggc ttagtggcct tctttggact tggcgatgac
     8881 gccgtcgccg gcccctccac cgaggttgtg gtggcgcgac cgggcttatt caactcggcg
     8941 ataacatcgc tggtgaaatc gctgttctcg cgcgcataca tcagcaccgg cacgccgttc
     9001 aggctcatcc cggacctgtc gaagacgagg tccatgttgt tcgccttgac tttgtccatc
     9061 accacatcgg tgatctcctt gacgatgcct tcgcgcatcc gcatggcctg ttcttgtaat
     9121 tgccgctcgc gcgtttgccg gaactcggtg atctcgcgct ccatgttttt aatgttcgcg
     9181 atcttgtcat cgcgctcctt cgctttttgc gatttggcat cagcactcaa ggcgggagca
     9241 tccagctgct tgttcaggtt gttgatttca tcgagtgctt ttttgtagcc gtcggcgcgg
     9301 tcgtcgtatt cctttttcgc cgcatttttt gcgtcgttaa ttttcgcctc cgcctctttg
     9361 gtcttgctat attctttgaa cgcgcgctgc atgtcgatag taccgatttt cagcccgccc
     9421 tgcgccaagg ccgccagcgg cgaggccaca gcggcggcga tcgccaatga gagaagtttt
     9481 ttcataagac agggatgggg taggttggtt ggaaacgcgt tagactgcaa ggcctgacat
     9541 tcgttcaaaa ttgatagcca acactgaagt tgaactggcc tccatagttg ttaccggcgc
     9601 gttgaatggg aatgccgtag tcgagtcgca gcggcccaat cggcaggtca agccgaatac
     9661 cgacaccgat atctgacgcc agattgttgt attcaagcgg cggcctggct gggtccctgc
     9721 gcggtttcgt ctgcgtcccg actgagaaat cgtaggcgtc aggattatcg aacccggtgt
     9781 catagaaaat tgcaccgcgt gctttttcga caatgggaaa cgtgtattcc actgtcgccc
     9841 gcgcaagggt cttgccgccg agtggttcgt cattcacatc cttcggcccg acgtcgcgga
     9901 actgaaagcc gcgcaaattg tttgcgccgc cgagaaagag tcgatcgaaa atcggcaccc
     9961 gatcaccgct gccccaagtg ttgactgtcg cggcctcgac attaaaaagc aggatgttgt
    10021 cccaccacaa atgaaagtat tgcgaggcct ccacgtccca gccgtagatt tggacgtcgc
    10081 cgccgagaaa accgccggcc acataaggcg tgaccgcgat gcgctgaccg cttcgcgtca
    10141 ggaagggatt gtcgcgccgg tcccaaacca gtccggcgag cacttcgctc ttgacggtcg
    10201 agcctttttc cagctcgagc gcagcggaag ccagcgggtc gacattgtaa atatcgaaat
    10261 cttccagccg atacgccagc gacgagtaga tgaaagggtt caacggcttg cggagctcga
    10321 ccatgaaacc gtagtcacgc tggtcgtagt acgaactgag ataattcgat tcgttgaaaa
    10381 acgcctgccc agtgagcgag agcggctggt caaatagcca cggctcggtg aaactgatca
    10441 atgcatcctt gcgctcggtt ccaacctgca ctcgaattct gaatttctga cccgccccgg
    10501 ttaaaaccgg ccagttcgtc acatcgaaat tgccctgtgt caattcgaca aagccgacca
    10561 gcttgtcgat cgaactgaag ccaccgccaa agctgaggga gccggtgcgt ttctcctgca
    10621 cttcgatggt caaatccttg cgcccgggca cgcccgtgtc ctcgggatag gtttcaacct
    10681 tggaaaaata gccgagattc tcgaggcgct tcttgctggt ttcaacgcgc accgtgttga
    10741 agatgtcacc gggggtcacc agcacttcgc ggcggattac cttgtccttg gtgcgtgtgt
    10801 tcccgacgac attaatgcgc tggataaagg agcgcgcgcc ctcctcgatc tgatagtgca
    10861 gatcgatcac cccgggcccg ccgggcgtcc cctgcggcgt aatcacaaga tcgacatagc
    10921 cgccgctgcc gtaagcgtca gccatggctt tggcgtcgtc atgcaatgct ttcggcgaat
    10981 agacgctgcc ttctttcatc ttgagcagcg cgcgaatctt ctcttccgtc gtcacctttt
    11041 cgccgctgat ggaaatttta ccgacgtgat attgcgtgcc ttcattcacc acgattgtga
    11101 tcgtcatgcg gccgtcggcg cgctgcctcc gcacgtcttt cagctcgaca tcgatgtagc
    11161 cgtggttctg ataaaactcg cggacgctat ccaagtcctg ctggaattgc gcctcgtcca
    11221 agcggccaga tttatcgaga taataaaaca gcgtcttgcc gcgggtcttc aactgcttgc
    11281 gcagtgtccg gtcgctgaag tgctggttac cttcaaaatt gatccggctg accgcgcctt
    11341 tcactccctc gttaatcgtg tagaccacgc gcgcggtccc gcgcttttcc tcgattggct
    11401 cgacccggaa ctgcacatcg gcttcgttga atcctttagc ccggtagatg tcgatgatgt
    11461 cctcccggcc tttctccaga tcttcttcct tcaccatcga gttgatcttg accgggatgg
    11521 ctttgcggat gcgtctcggc ttcaccgcct gcgccccgtc aatcacgatc tcacgcacga
    11581 ctgagcgcgt ctgcaccgct acaatcacct tcacgccatc ggcctgcggt tgcgcaaaaa
    11641 tgcgcacatt taaaaccgcg ccggtcttgt agagattttt aatgtcctcg ttgaccacct
    11701 cctcggagta cggccgacct attcgcgtcc ggatttgcgc cagaattcgc tctttgctga
    11761 tggtcgccgg cccgctgtat tgcacgtcga tcgagcggat aatcggcggg gtctccgcaa
    11821 ccgggttttg cgtccgcgcc gaagtggcaa acaacacgaa ggcaatcgcg acggaaaatg
    11881 aaaacgccgg ggagcggagc ggtcggcgaa tcaatctcat cggttaaaca agacagtccc
    11941 gcgccgagtc ctgacgcggg acacgcgcct attagagaat aagcgaagag tcgtcaaggc
    12001 gggatttgtg aaggaaggcg cgccggcggc ctgttgatag acagcggccc gagactttta
    12061 gttcaaaagc tcgtaccagt tattccgccg ccgcggttcg tagccggcgt cccgaataag
    12121 cgattcgatc tgttgcgcgt tgaggcgaaa tgtcgtgccc gcactgctca ccacgttctc
    12181 ttccatcatc actgacccga aatcgttcgc gccatatttt agcgcgacct gcccgatgcc
    12241 cggcccctgc gtcacccacg agctctggat gttctcgata ttgtccagga agattcgcga
    12301 gagcgcctga gtgcgcaggt attcggccac acccgttgca ttgtcgatct tcaaggccgt
    12361 gtgctgcggt tgaaacgtcc agcagatgaa cgcggtgaat ccagcgcttt tttcctgctg
    12421 atcgcgaatg cgctgcaaat gctcgattcg gtcttcaatc gtctcgacat gcccgaacat
    12481 catcgtcgca ctcgatttca atccgagctc gtgcgcgacc tgcatcactt ccagccagcc
    12541 acgactgtcg attttcagcg gcgaaatttt tctgcgaaca cgatcgacca aaatttcgcc
    12601 gccgcctccg ggaatcgaac cgaggcccgc atttttgaat tccgcaatca cttcgcgcaa
    12661 cggcatccgg aacgtctccg caaaatgctg aaactccggc ggactgaatc cgtggatgtt
    12721 gatgtgcgga tatttttcgc ggatgtgccg caggagatcg acataccact ggaacggcag
    12781 ctttgggtga tgcccgccct gcattagaat ctggaccccg ccagccgccg tgagttcgtc
    12841 gagtttgcgg tcgatctcct caaacgacag cacgtagtga tcggcatcct tttccgtgcg
    12901 ataaaacgcg cagaacttgc agtacacatt acagacgttc gtgtaattga tgttgcgatc
    12961 gacgatgtaa gttacgatgg cattgccgcg gccgttgtac gatcgacatt tcgcctgccg
    13021 ccgccgctcg tctgcgagcg cgccgagctc ctccaggggc aatccataaa gttccagtgc
    13081 gtcgtcagac gaaatccgtt cgcccgcgag gactttttcc gtcagcgcct ctcggttaat
    13141 cgcaaccatc tcggtccgag cctaaatgat cgtggctctt ttcgcacgca tgttcacacg
    13201 cgaatttgta ggacaagcgc accggttgcc gctttttaat tggcaggcgg accgcctgcc
    13261 ctgcaacgtt gtcacgtgcc aaaatccgtg gcaatcgtcg gtgccggcgt ttccggactg
    13321 acctgcggcg tgctttttgc ggagcgccga tatcgcaccg cgattttcgc cgagcagagt
    13381 gggcaggaaa cgacatccgg cgctgcggcc gcgctttggt tcccttacga ttgcgagccg
    13441 cgcaccaaag tcgtcgcgtg ggcgctcaca acctaccgag tacttgtcgg tcttgcttcc
    13501 gatccgcgct ccggtgtttc catgctgcaa ttgcgccaat attcgcgcac cggcgctctg
    13561 gctattcccg agtgggcgca ttcgttaggc gcgcattgcc tatctctcat tccgagcgct
    13621 cgccgcgccg tagcgcagcg aaggcgggaa gtcgaggaat ctctcacttt tgcttccatc
    13681 tttaaaagcg gtttcgccat gaaggtcccg ctaatggaca cgacaattta cctcgattac
    13741 ctcacgaacc ggtttcgcgc gcgcgggggc gagatccacg ccggtgtacg tttcgacggg
    13801 ctcgatgcca tcgaccctga cttcgacatc gtaatcaatt gtgccggcat tggtgcgcgc
    13861 gaactcttgc acgatgtcga tcttgagccg catcgcgggc aagtcgcggt cgtcccgaag
    13921 atcaataatc tctcctgcgc ggttgtctgc gacgaagagc cactcatgta cgcgattccg
    13981 cgcacaaacg attgtctctt cgggggtacg aacgagctca gcgacaatct cgccatcgac
    14041 cgcgacacga cctcgcgcat cgttaacgaa tgcgtccgga ctctgaaaat cgacaagccg
    14101 cgagtattag ccgagcgcgt cggtctccgc ccgtatcgca actcaggcgt ccgccttgaa
    14161 cgcgataaac tgcgcgacgg ccgcaccatc atccacaact atggccatgg cgggtctggc
    14221 ttcacccttt cctggggctg cgctgaagaa gtctttaaga tcgcggatc
//
LOCUS       DASTDG010000241         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1147421, whole genome shotgun sequence.
ACCESSION   DASTDG010000241 DASTDG010000000
VERSION     DASTDG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147421"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFO30_08000"
     CDS             <1..1342
                     /locus_tag="VFO30_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9419268.1"
                     /translation="GNDIVADDADTLAAHSGDKWFAAETPEVVVFARSTRQVVELLKF
                     ASRERIPVTARGGGFGYVGGCVPAQRGIALSLAPMNRIKEVSFSDAIAVVEPGVMTAD
                     LKAAVRAQKLFYPPDPASMKNCTLGGNVATNAGGPRCLKYGVTRNYIVGLEVVLASGE
                     VLRTGGRVHKNKTGFNLIGLFVGSEGMLGIVTEITLRLLPLPPARATLSAAFAKMSQA
                     AAAVNEIFAAGFLPSSLEIADSFTLAAARENIGQALVPPGNAHLLVDLDGQQESVRSE
                     AAAVRELLARKNPNALEMATGEEDSEKLWALRREFSESLRATGLTKLNQDVVVPRSHI
                     VDLIEFAENLQAKHGFPIACFGHAGDGNMHVNIMADHYNRDAAVRAKVEHALDELFAQ
                     VLAWGGVITGEHGIGLAKKRWWPQATSDVSRELHAKVKHVLDPNNILNPGKFLD"
     gene            1609..2535
                     /locus_tag="VFO30_08005"
     CDS             1609..2535
                     /locus_tag="VFO30_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419269.1"
                     /translation="MKPGTITHTLGVLLSSVSSLFAHSAPTQGESAKVKMSKRSEGSP
                     QPDGMLSLDSIMFPPGHLLSRSSPPASFSTPGSPTAAQSAQQQESRFPWKCSIVTTVF
                     WIGEEPTANNPVPNRASSWDKQWTKSYGGFDDPNPAHRRDFIPVKFTPRQNPFYCALP
                     YNDKAGTGHKPEAPHVVPWFNQAYQGPGVSTCKGHWVEIRKGNRVAYAQWEDAGPFRT
                     DHWQYVFGDERPKPNLNKGAGLDISPAVRDYLGLAPTDVTDWRFVDFSEVPRGPWAKL
                     GENNTFVMNERQRGELVAQAKTTGAGQSTAVE"
     gene            2565..>3245
                     /locus_tag="VFO30_08010"
     CDS             2565..>3245
                     /locus_tag="VFO30_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419270.1"
                     /translation="MKSARVVYTVFVAALFAAQPPALAQSDAAGKVLTGKAAMGDWTS
                     DAPGVRRKIAVEDLPPPGSNALAINPPRVVDRPPGAQLYVPAGFNIDMFASGFRDPRF
                     LLTAPNGDVFVSESHANQIKVIRGAHAESVQVFAEEGLNKPFGLAFYPPGGEPQFLYV
                     ANTDGVIRFPYRNGDLKARRPAEKLPATLSGGAGFLRSGGHWTRDIVFSPDGKKMYVS
                     VGSRSNNSD"
BASE COUNT          662 a          933 c         1012 g          638 t
ORIGIN      
        1 cggcaacgac atcgtcgccg acgatgccga tacgcttgcc gcgcacagcg gcgacaagtg
       61 gttcgccgcc gagacgccgg aagtggtcgt cttcgcaaga tcgacaaggc aggtagtcga
      121 gctgttgaaa ttcgcttcgc gcgagagaat tccggtgacc gcgcgcggag gcggtttcgg
      181 gtacgttggc ggctgcgtgc cggcgcaaag gggaattgcg ctttcactcg cgcccatgaa
      241 ccgcatcaag gaagtcagtt tcagcgatgc gatcgcagtc gtcgagccgg gtgtaatgac
      301 tgctgatctg aaggcggcgg tgcgcgcgca gaaactattt tatccgccgg atccggcgag
      361 catgaaaaac tgcacactgg gtggaaatgt tgcgacgaac gcgggcggcc cgcgttgttt
      421 gaagtacggc gtcacgcgca actacatcgt cggtctggaa gtcgtgctcg ccagtggcga
      481 agttttgcgg actggcgggc gcgtgcacaa aaacaagacc ggattcaacc tgatcggatt
      541 gttcgttggt tcggagggaa tgctgggcat cgtgaccgag atcacgctgc gtttgctccc
      601 gctcccgccg gcgcgcgcga cattgtcggc tgcgttcgcg aaaatgtcgc aagccgccgc
      661 cgcggtgaac gaaatttttg ccgctgggtt tttgccgtcc tcattggaaa tcgccgacag
      721 cttcaccctc gcagctgccc gcgaaaatat tggccaggcg cttgttccgc cgggcaacgc
      781 ccatttgctt gtcgatttgg atggacagca ggagagcgtt cggagtgaag cggcggctgt
      841 gcgcgaattg ctcgcacgca aaaatccgaa cgcgctggag atggcgactg gcgaagagga
      901 ttccgagaag ctctgggccc tgcgccgtga gttcagcgaa tcgctgcgcg cgaccggcct
      961 aacgaaattg aaccaggacg tcgtcgtgcc gcgcagccac attgtcgatc ttatcgagtt
     1021 cgcggaaaac ctgcaggcga aacacgggtt cccgatcgcc tgcttcggcc atgccggcga
     1081 cggcaacatg cacgtcaaca tcatggctga tcattacaac cgcgacgctg ccgtgcgggc
     1141 caaggtcgag cacgcgctgg atgagttgtt cgcgcaagtc ttggcttggg gtggagtgat
     1201 taccggcgag catggcattg gtctggccaa gaagcgctgg tggccacagg cgaccagcga
     1261 tgtctcgcgc gagctacacg caaaagtgaa gcacgttctc gatccgaata acatcctgaa
     1321 tcccggcaaa tttctcgact gacggcggag attcttgatt ggtttggtgt ggctctcatc
     1381 ctaatcctct ccttcgaacg gagaggcgta cgcacccagc gctggtgagg gtaaaaggaa
     1441 caatagtttt cgcagcgcgc ttgacggaag cgcatttacc gccacctttc gggtacacag
     1501 ggcaggtgaa aggcgagcgc gatctacgcg cagtcgcctt gccgcagccg aagtaaagga
     1561 aagggcaacg tgttgcgctg gcgaaggttg cgctgaggga acttcgaaat gaaaccagga
     1621 accatcaccc acacgctcgg agtactcttg tcgtcggtct cttctttgtt cgcgcactcg
     1681 gcgccgacac agggcgaatc ggcgaaggtc aagatgagca aaagatcgga gggctcacct
     1741 cagcctgacg gaatgcttag tcttgactcg atcatgtttc cgcccggcca tttgctttcg
     1801 cgatcctcgc cgcccgcctc cttttccacc ccaggatcac cgaccgcggc ccaatccgcg
     1861 cagcaacagg agagtcgttt tccatggaag tgcagtatcg tgacgactgt gttctggatc
     1921 ggcgaagagc cgacggctaa taacccggtg ccgaaccgtg cgagctcctg ggacaagcag
     1981 tggacgaaaa gttatggcgg attcgacgat ccaaatccgg cgcaccggcg cgattttatc
     2041 ccggtaaaat ttacaccgcg gcaaaatccg ttttactgcg cgttgcccta caacgacaaa
     2101 gcaggcaccg ggcacaagcc tgaggcgccg cacgtggtgc cgtggtttaa ccaagcctat
     2161 caagggccgg gcgtctccac atgcaaaggc cattgggtcg aaatccgcaa aggcaaccgt
     2221 gtggcctatg cgcaatggga agacgccggg ccgtttcgca ccgatcactg gcagtacgtt
     2281 ttcggcgacg agcgaccgaa acctaatctg aataaaggcg caggcctcga tatctcgccg
     2341 gcagtgcgcg attacctcgg cctcgcgccg acagacgtga cggactggcg atttgtcgat
     2401 tttagcgaag tgccgcgcgg gccgtgggcg aaactcggcg agaacaacac cttcgtcatg
     2461 aacgagcgcc aacgcggcga acttgtggcc caagccaaga cgactggtgc tgggcaatct
     2521 accgcagtcg agtagcaggt tcgccgcagg tgagacgcgc ttgtatgaag tcggcgcgag
     2581 ttgtttacac agtttttgtc gcggcacttt tcgctgcaca gccgccggcg ctcgcccaat
     2641 cggatgcggc tggaaaagtt cttaccggca aagcggcgat gggcgattgg acgagcgacg
     2701 cgccgggggt gcgtcgcaag atcgccgtcg aagatttgcc gcctccgggt tcgaatgcat
     2761 tggccatcaa tccgccgcgc gtggtcgatc gtccgccggg cgcgcaactg tatgttcctg
     2821 cgggattcaa catcgacatg ttcgcaagcg ggtttcgcga tccgcgcttt ctgctcaccg
     2881 cgccgaacgg cgatgttttc gtgagcgaga gtcacgcgaa ccaaatcaag gtgattcgcg
     2941 gtgcccatgc ggaatcggtg caggtgtttg ccgaggaggg attgaataag ccattcggcc
     3001 tcgcgtttta cccgcccggc ggtgagccgc agtttcttta tgtcgccaac accgacggcg
     3061 tgattcggtt tccttatcgt aacggcgact tgaaagcgcg ccgcccggcc gaaaagctcc
     3121 ccgcgacgct ctccggcggc gcaggttttc tgcgcagcgg cggccattgg acgcgcgaca
     3181 tcgtgttttc gcccgacggc aaaaagatgt acgtctcggt cggttcgcgc tcgaacaatt
     3241 ccgat
//
LOCUS       DASTDG010000242         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1569430, whole genome shotgun sequence.
ACCESSION   DASTDG010000242 DASTDG010000000
VERSION     DASTDG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569430"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFO30_08015"
     CDS             complement(<1..726)
                     /locus_tag="VFO30_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9419271.1"
                     /translation="MRTEPLQIPNFVNGEFVAPVSGRYLDNIEPATGKPYSQVADSGK
                     EDVDLAVAAAENAFATWSGTVAAERSKALLRIADLIERDLEKFARAESIDTGKPITLA
                     RTLDIPRAVANFRFFATAILHTETEAHITDNVAFNYTLRQPRGIAGLISPWNLPLYLL
                     SWKIAPAIAVGNTAIAKPSELTPMTALMLCEVARLASLPSGVLNIVHGTGPNVGAAIT
                     SHPKINTISFTGGTVTGKKVAEAC"
     gene            846..1073
                     /locus_tag="VFO30_08020"
     CDS             846..1073
                     /locus_tag="VFO30_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419272.1"
                     /translation="MRQLQLLLFCAVFLAGCETTQPPGMGPEEQKPLTHLQQQQRQQQ
                     EQGAQMDEADRNLWNAQMDRLNQGTNPANRY"
     gene            1101..1490
                     /locus_tag="VFO30_08025"
     CDS             1101..1490
                     /locus_tag="VFO30_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET9419273.1"
                     /translation="MAERQRHREIPAEHRAQEHLANERTFLAWVRTNIALISLGFVLA
                     RVGPWLDHGPGGARTTTRTPALGFGLVVLGALLTLLAAWRYDAVNREIESGLVKTDRA
                     LVWFVALGVALLSAALIAYMLAARPAE"
     gene            1496..2836
                     /locus_tag="VFO30_08030"
     CDS             1496..2836
                     /locus_tag="VFO30_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9419274.1"
                     /translation="MKLPELPPFDYQPQKYSGPSADEVLRLRKEFLNPGIFLYYKKPL
                     MLVEGKMQYVWDEHGRRYLDALGGIVTVSVGHCHPDVVHAANKQNETLQHSTTVYLHP
                     NIADYARALAKKMPGDLKVCYFVNSGSEANDLALLMARAYTGSYDVIALRNAYHGGGT
                     LTMGLTAHRTWKFNVPHSFGIHHAMTPDPYRGLWGRDDADAGSKYADDVKNLIDFGTT
                     GQVAAFIAESIQGVGGVVVFPDGYLKHVYEHVRAAGGLCIADEVQAGFGRTGTHYWGF
                     QTQGVIPDIVTMAKGIGNGCPLGAVVTTPAIAQTLGARLHFNTFGGNPVVCAQGKAVL
                     EVIERERLQENALKIGNRILAGLDELKAKHNIIGDVRGKGLMLGIELVKDRQSKEPAR
                     EECAQLLERAKELGLLLGKGGLWGQTIRFSPPMCVNEADADFLLQVLDAALSEL"
     gene            complement(3217..>3846)
                     /locus_tag="VFO30_08035"
     CDS             complement(3217..>3846)
                     /locus_tag="VFO30_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419275.1"
                     /translation="LGDYAEKNGNIDIAEAAYTNAAIVAPKLRIAQQGRLRIAQRKPD
                     TRKLHSILAEMLAIWPNDSAVQNDEAYTRLLLLMPEQQSREVGDQPSPSSGEPGNREP
                     PASNEELERTEQLAEQLIKRDPASLPHLTLLALARLRQGRVEDALRVYENIQVAPNAL
                     TPSALAVHAAVLEATGHHQDAQSELDKIKPDQLLPEEQALIEKAEKPKD"
BASE COUNT          801 a         1117 c         1150 g          778 t
ORIGIN      
        1 gcatgcttcg gcgacttttt tgccggtaac ggttccgccg gtgaacgaga tcgtgttgat
       61 cttcggatgc gacgtgattg ccgcaccgac atttggtccc gtgccgtgca caatgttcag
      121 cacgccactc ggcaaactgg ccaagcgcgc gacctcgcac agcatcagcg ctgtcatcgg
      181 cgtgagttcg ctcggcttag caatagccgt gttcccgact gcgatcgccg gcgcgatttt
      241 ccagctcaac aagtaaagcg ggagattcca gggcgagatc agccccgcga tgccgcgcgg
      301 ctgacgcagc gtgtaattga acgccacgtt gtcggtgatg tgcgcttcgg tctcggtgtg
      361 gaggatcgcg gtcgcgaaga atcgaaagtt cgcaacggcg cgcggaatat ccagtgtgcg
      421 cgccagtgtg atcggcttgc ccgtgtcgat cgattcggcg cgcgcgaatt tttcgaggtc
      481 gcgctcaatc aggtcggcga tacgcagaag agccttcgag cgctccgctg ccaccgtgcc
      541 cgaccaagtc gcgaacgcgt tctccgccgc ggcgactgca agatcgacat cttctttccc
      601 gctgtcggcg acctgcgagt acggcttgcc ggtagcgggc tcgatgttgt cgagataacg
      661 tccgctgacc ggcgcgacga actcgccgtt gacgaaattt ggaatctgaa gcggctcagt
      721 gcgcacgaga gaataaaggt tgtagagccg ctcgtgccaa gcgcctgttt agtaaatcga
      781 gtggacctcc cacccgaatt tgatacaagc aacgctacaa cgccggaatc ctgtatattg
      841 agccgatgag acagcttcaa ctccttctct tttgcgcagt gttcctggcc ggttgcgaaa
      901 cgacacaacc ccctggaatg ggcccggagg agcagaaacc cctcacgcac ttacagcagc
      961 agcagcggca gcagcaggag cagggagcgc aaatggacga ggcggaccgc aatctttgga
     1021 acgcgcaaat ggatcgcctg aatcagggga cgaatccagc gaatcggtac tgagcgagtc
     1081 gccaaagcag cgccgaacag atggccgagc gacagcgcca ccgcgaaatc ccggccgaac
     1141 atcgcgcgca agaacacctc gcgaatgagc gcacttttct ggcttgggtg cggacgaata
     1201 ttgcgctgat cagcctcggc ttcgtgctcg ctcgcgtcgg gccctggctt gatcacggcc
     1261 ccggtggagc acggaccact accagaacac cagcgctcgg attcggtctc gtggttctgg
     1321 gtgcgctgct gactttgctc gccgcgtggc ggtatgatgc ggtcaatcgc gagatcgaat
     1381 ccggcctcgt gaaaaccgat cgcgcgctgg tgtggtttgt cgcgctcggg gtggccctct
     1441 tatccgccgc actaatcgct tacatgttgg cggcacgtcc ggcagaatag caaagatgaa
     1501 attgcccgag ctgccaccgt tcgattatca accacagaaa tacagcggcc cgtccgccga
     1561 tgaagtcttg cgcttgcgga aagaattcct gaaccccgga atttttctgt actacaagaa
     1621 gccgctcatg ctcgtggagg ggaaaatgca atacgtctgg gatgagcacg ggcgccgtta
     1681 cctcgacgcc ctcggtggga tcgtcacggt cagcgtcgga cattgtcacc cggacgttgt
     1741 ccacgctgcg aataagcaga acgagacgct gcaacattcg actaccgtct atcttcaccc
     1801 gaacattgcc gactacgcgc gcgcgctggc gaagaaaatg cccggtgacc tgaaggtctg
     1861 ctatttcgtc aactccggct ccgaagccaa cgacctggcg ctgttgatgg cgcgtgctta
     1921 caccggtagc tacgacgtga tcgcattgcg aaatgcgtat cacggcggcg gaacattaac
     1981 catgggtctg accgcgcatc gcacctggaa atttaacgtg ccgcacagct tcggaatcca
     2041 tcacgccatg acgcctgatc cgtatcgcgg attgtgggga cgcgatgatg ccgatgcagg
     2101 aagcaaatac gctgacgacg tgaagaatct gatcgacttc ggcacgaccg ggcaagtcgc
     2161 cgcgttcatc gccgagtcga tccagggtgt cggcggggtg gttgtatttc ccgacggcta
     2221 cctcaagcac gtttatgagc atgtccgcgc ggccggcgga ctttgcattg ccgatgaagt
     2281 gcaggcgggc ttcggccgca ctggaacgca ttactgggga ttccaaaccc agggcgtcat
     2341 tcccgacatc gtcacgatgg caaaagggat cggcaacggc tgtccgcttg gcgcggtcgt
     2401 cacaacgcca gccatcgcac agacgctcgg cgcgcgcttg cacttcaaca ccttcggtgg
     2461 caacccggtt gtttgtgcgc agggcaaagc cgttttggaa gtgatcgagc gcgagcgcct
     2521 ccaggaaaac gcgctcaaga tcggcaaccg cattctggcc gggctggatg agctaaaagc
     2581 aaaacacaac atcatcggtg acgtgcgcgg gaaggggctc atgctcggga tcgagctggt
     2641 gaaagatcga caatcaaagg agccggcacg cgaagagtgt gcacagctgc tcgagcgcgc
     2701 gaaggagctc ggattacttc tcggcaaagg cgggctgtgg ggacaaacca tccgcttctc
     2761 tccgccgatg tgcgtcaacg aagctgacgc cgattttctg ctgcaggttc tcgacgctgc
     2821 gctttcggaa ttgtaaccga accatactga gctttagtcg tcgactggcg atccgtgaag
     2881 ctcagaaaaa gcttctgagc gtcagcattc cggagtgtca gcggtttagt ttcccggtgt
     2941 ttagcgtttg cagatcggcg tgtgaatagt tgagcgtcga ctgcaacgtc gacaatcaac
     3001 tcgagggtga taaagcgaca tcgcggggag cgggacagcg aaaagccgca aaatgtagga
     3061 gaaatcccga tgtggcccaa aggggaacac tgagccaata agttgtgaac aacttgggca
     3121 aaaacctgaa acgctaaatc gtgaaatacg gaccgacgcg aatcctcgcc aaacctaaaa
     3181 cgccgacttg ggcgggcatc tcgaaatttc agcttttcag tctttcggct tttcagcttt
     3241 ttcaatcaat gcctgctcct ccggcagaag ctgatcgggc ttgatcttgt cgagttcgct
     3301 ctgcgcgtct tggtggtggc cggtcgcttc cagcaccgca gcgtgcactg ccagcgcaga
     3361 cggcgtaagt gcgttcggtg cgacctggat gttttcatag acacgcaagg catcttcgac
     3421 gcgcccttgg cggagccgcg ccagcgcgag caacgtcaga tgcggcaaac tcgccgggtc
     3481 gcgcttgatc aattgttcgg ccaattgctc ggtccgctcc agctcctcat tacttgccgg
     3541 cggttctcga ttccctggct cgccggagct cggcgaaggc tgatctccga cctcgcgcga
     3601 ttgctgctcc ggcatcagca gcagcaatcg cgtatacgct tcgtcattct gcacggctga
     3661 gtcgttaggc cagatcgcga gcatctcggc gagaatggag tgcagcttgc gcgtgtccgg
     3721 ttttcgttgc gcgatgcgca accggccctg ctgcgcaatg cgcaacttcg gtgcaacgat
     3781 cgccgcatta gtgtaagccg cctcggcgat gtcgatgttg ccgttctttt ccgcgtaatc
     3841 acccaa
//
LOCUS       DASTDG010000243         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_611105, whole genome shotgun sequence.
ACCESSION   DASTDG010000243 DASTDG010000000
VERSION     DASTDG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611105"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..938)
                     /gene="tpiA"
                     /locus_tag="VFO30_08040"
     CDS             complement(171..938)
                     /gene="tpiA"
                     /locus_tag="VFO30_08040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002144919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9419276.1"
                     /translation="MRKKIVAANWKMNMTQAEAAAFMKSLLLDLGDITDVEVVIIPPF
                     TAIAKVMEALGSSQTIKVGAQNMHWEPNGAFTGEISAALLRDLFVRYVVLGHSERRIL
                     FGETDEIVNQKVRAAHEATLRPIVCIGETLEQRDKGNVEKILSIQLRRSLAGLNEKEL
                     QETVIAYEPVWAIGTGRNATPDQAQEAHAFIRQRLRELSDETTAERVRIQYGGSVKPE
                     NARELMNQADIDGALVGGASLDPHSFAQIVNAAREEV"
     gene            complement(938..2149)
                     /locus_tag="VFO30_08045"
     CDS             complement(938..2149)
                     /locus_tag="VFO30_08045"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9419277.1"
                     /translation="MRKLSVRELEVRGKRVLVRVDFNVPIDERDGKIDIVDDTRIRAA
                     LPTINYLREHGAKTILMSHFGRPKGKPVEKYSLRPIGEYLHNLIKHPVAFAHETIGKI
                     PEEIIADMKQGDVMLLENLRFQPEEEANDARFAEALAKFGDLYVNDAFGAAHRAHAST
                     VGVTKFISPSAMGLLMEKELKYLHEELDKPAKPFVVIIGGAKVSDKIGVLEALIEKAD
                     AILIGGAMANTFLQAQGISIGASRVEADKTDLSREVVDLAKARGVKFLLPIDVVIARE
                     IRAGAPIQNTSRLSPKHGIEEGWQAVDIGMATVALYREEIEQAKTILWNGPVGIFEIP
                     DFAEGTMAIAEALAESGATTIIGGGDSVTAVRQAGLADKMTFISTGGGASLELLEGKE
                     LPGVAALTDKE"
     gene            complement(2152..>2690)
                     /locus_tag="VFO30_08050"
     CDS             complement(2152..>2690)
                     /locus_tag="VFO30_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9419278.1"
                     /translation="LQQPLRRNDGVVGKIGRLAHARAPAHARTMKTYFDHERLDVYRE
                     AIGFCGWVGELIGGIATKAAAKDQLDRASTSIPLNIAEGNGKFSAADRARFLEIARGS
                     ALECAACLDVLAVRKLIAGDQIIPAKEQLVRIVNMLMGMLKRFSGRAEFLREDEPTYY
                     AEHEHEYEQEQESRQEQE"
BASE COUNT          530 a          866 c          681 g          613 t
ORIGIN      
        1 tctccgcgca aaagatcgac aatcgctggt tcctgaaaca aatgcgggtt gaagagctgc
       61 agcccggtac gaatcacgtc gtggcgcgaa cgtacctcga gatcaagaag tagcgtgctt
      121 tgtagccacg gccctatggg ccgtccacgt gacgcgccac cggcgcgtgg ctacacttct
      181 tcgcgcgccg cattcacgat ttgcgcgaag ctgtgcgggt cgagactggc gccgccaacg
      241 agggcaccgt caatatcggc ctggttcatg agctcgcgcg cattctccgg tttgacgctg
      301 cccccgtact gaatgcggac ccgctccgcc gtcgtctcgt ccgacagctc gcgtaacctc
      361 tgacgaatga atgcatgcgc ttcttgtgct tggtccgggg tcgcgttgcg gccggtgcca
      421 atcgcccaga ccggctcgta agcgataacg gtctcctgaa gttctttttc gtttaaaccg
      481 gcaaggctgc ggcgcagttg gatcgacaat attttttcga cattgccttt gtcgcgttgc
      541 tccagcgtct cgccgatgca gacgatcggt cggagcgtcg cctcatgcgc ggcgcgaacc
      601 ttctggttta cgatctcgtc ggtttcgcca aacaatatcc ggcgctcgct gtgcccgagc
      661 acgacatagc ggacgaatag atcgcggagc aacgccgcgc taatctcacc tgtgaacgcg
      721 ccgttcggct cccagtgcat gttctgcgcg ccgaccttaa tcgtctggga cgaaccaagc
      781 gcttccatta ccttcgcaat cgcagtgaat ggcggaatga ttactacctc gacatcggtg
      841 atatcgccga ggtcgagcag cagcgatttc ataaatgcag ccgcttcggc ctgcgtcatg
      901 ttcatcttcc aattagccgc gacgattttc ttgcgcatca ctctttgtcc gtcaacgccg
      961 cgacgccggg cagttccttg ccctcgagca actcgagcga tgcgccgccg ccagtcgata
     1021 tgaaagtcat cttatcggcg aggccggctt gtcgcaccgc cgtcaccgag tcgccgccgc
     1081 cgatgattgt cgtggcgccc gattcagcca atgcttccgc gattgccatc gtgccttccg
     1141 cgaaatccgg aatttcgaaa atgccgacgg gtccgttcca caaaatagtt ttcgcctgct
     1201 caatttcctc gcgataaagc gcgactgtcg ccatgccgat atccactgcc tgccagccct
     1261 cctcgattcc gtgtttcggc gataagcggc tcgtgttttg aatcggagcg ccggcgcgaa
     1321 tctcccgcgc aatcacaacg tcaatcggca gcaaaaattt cacgccgcgc gcttttgcaa
     1381 gatcgacaac ttcacgcgag agatcggtct tgtccgcctc gacacgactc gcgccgatcg
     1441 aaattccctg cgcctgcaaa aacgtgtttg ccatcgcgcc gccgatcagg atggcgtccg
     1501 ctttttcaat cagcgcctcg agcacgccaa tcttgtccga gactttcgcg ccgccgatga
     1561 tcaccacgaa cggcttcgcc ggcttatcca gctcctcatg gagatacttc aactccttct
     1621 ccatcaagag ccccatcgcc gatggcgaga taaatttcgt cacgcccacc gtcgacgcgt
     1681 gcgcgcggtg cgccgcgccg aacgcgtcgt tcacgtaaag gtcgccaaac ttcgccaacg
     1741 cctcggcaaa tctcgcgtca ttcgcttctt cttccggctg aaaccgcaaa ttctcgagca
     1801 gcatcacgtc gccttgcttc atatccgcga tgatctcctc gggaattttg ccgatcgtct
     1861 catgtgcaaa cgctactgga tgcttgatca aattgtgcag gtactcgccg ataggtcgca
     1921 gcgagtattt ctcgaccggc ttccctttcg gccgcccgaa atgcgacatc aaaatcgtct
     1981 tcgcgccgtg ctcgcgcaga taattgatcg tcgggagtgc tgcgcggatt cgtgtgtcat
     2041 cgacgatgtc gatcttgccg tcgcgttcgt cgatcggcac gttgaaatcc acgcgcacca
     2101 gcacgcgctt cccgcgcacc tccagctcgc gaaccgacag cttccgcatt cctactcctg
     2161 ctcctgacga gactcttgct cctgctcata ctcatgctca tgctcggcat agtaggtggg
     2221 ttcatcctcg cgtaagaatt ctgcccgacc agaaaatcgt ttcagcattc ccatgagcat
     2281 attcacgatt cggacgagct gctccttcgc gggaataatc tgatcgccag caatgagctt
     2341 tcgcactgca agtacatcca aacatgccgc gcactccagc gcggaacctc tcgcgatttc
     2401 cagaaatctg gcgcgatcag ccgccgaaaa tttcccgtta ccttcagcga tattcagcgg
     2461 aatgcttgtc gaggcgcgat caagttggtc tttggccgcg gctttcgttg caattccgcc
     2521 gatcaactct cctacccatc cacaaaagcc aattgcttcc cgataaacat caagccgctc
     2581 gtgatcgaag tacgtcttca tagttcgagc atgagcagga gcacgagcat gagcaagacg
     2641 ccctattttc ccaacaactc catcatttcg acgcagcggt tgctgtagcc
//
LOCUS       DASTDG010000244         6079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_125107, whole genome shotgun sequence.
ACCESSION   DASTDG010000244 DASTDG010000000
VERSION     DASTDG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125107"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VFO30_08055"
     CDS             <1..1560
                     /locus_tag="VFO30_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9419279.1"
                     /translation="NFESQNVELAKLFTDLGAKPPVSGTVNVKLDAQGTLADLSARLD
                     VQMRGLRSERLPNLEPASFDLVAQSQHDQLAISGKLQQAKIQPLEITANLPLRLAKTL
                     RERKFADETPLSAKIRLPRSSVNFVRQFVPAVQELDGDAALDVDVTGTIGRPVFTGSG
                     DITINVARASDVTLPALRDFKARLNFARDTLSLDQFGGELSGGRFTMNGRVTLPKLTA
                     ANLDLQLRADSALLARNDTLTARADADIKITGPLNSANVTGTVAMTNSHVLKNLDLLP
                     IGLPGRPAPQPPSARPEFSFPDPPLRDWKFDVAIKTKDPVLIRGSLANGGAITDVHIG
                     GTGLHPELQGTVRLENVEATLPFSRLEISSGFVYFDPNDSFNPRLDLHGTSLIHDHTI
                     HVYIYGTALAPEAIFTSEPPLPQEEVISLLATGTTREELTGSNNVLAGRAAMLLVQQL
                     YRKVFKKGQPTQTTSVFDRLDVDLGTVDPRTGRQQATARFKVNDQFVLVGDIGVGGDY
                     RGMVKYLIRFR"
     gene            1560..3743
                     /locus_tag="VFO30_08060"
     CDS             1560..3743
                     /locus_tag="VFO30_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9419280.1"
                     /translation="MRSLCLLIAACSIGLAGKLAAQTAVDVPQSERPEAKQKQQQAIA
                     KQQEKHAQRTSTIEFQGATAFGEKELRSQLKEQITTIDQYGLTPARADDLAFFLEVFY
                     RKHGYVKVNVHYVIESGDRLRLLINEGTLMTLGRVVFEGNVGEPTEKLFDFAVGPTRE
                     RYSKLQKALPFVAADLEEGAELVDRLYVAEGYLDAVVDKPRYVFHDETNQVDAIISIH
                     EGRQYFFGDVSFSGRMIYDPEALRGQIVDLLQQPYTDARVADIPRRLQAYFKSRGYYD
                     VKVEATGDPEAAVKGRVPVQIAIAAGPLYHFGDVTVSGLSRLRPSFVTRRFRNLAGQT
                     YSPEVLDEKFRTLMRTGLFNILQIKPVPVDENELRLDISAEEAKSKEFGFSVGYGTFQ
                     GGIVGVQYRDRDLFGYGRPLTTSIELSQRGYKGEILYEDPFFFDTDLSFRNQLAALTF
                     DYDGYSKFEVGDRIELTRKFTKQYEAGLVFAVRHVELTSVSINQRFLGQTSYFINTLG
                     FTQTLDLRESPLVSPRGLVVNTTLDAATGAFGSEIDLIRGTTRVAYFLPFGPKPLTPG
                     VVEDRTESSLQRWFHQSLLALGARAGLAHSLNNSGPDEPTTLPIDERFFNGGATTVRS
                     YGERDLGPHDPKGNPIGGEFFTVFNVEYTFPILGELQGALFFDAGDLLPTSEEPGVDD
                     MHYALGMGLRYKLPIGPIRLDYGVNPDRQPGEDIGAFHFSFGFAF"
     gene            3740..4774
                     /locus_tag="VFO30_08065"
     CDS             3740..4774
                     /locus_tag="VFO30_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419281.1"
                     /translation="MNATYLLPIRRTIFAETEAADLREYFAMLAHAGCAVIVVDGSPP
                     EIFAQHHEFWSDIGRHEVVDPRFKFLNDKVNGVHTGVALATNDKIILADDDILYTAAD
                     LEQICALLDRFEVVRPQNYFVASASSRCLYEHGQDARTTFPPWAKMESARMLINRATL
                     RAADYPSTCAFRRQAFLSAGEYDGDVLFDNEELIRHFARSGARICYANDLFIRKRPPT
                     FRKWLEQRPRQAYEDFGLRFKTVLFASLLPIGIVLAVSDARRLSIFLAPIFIAFAGWL
                     RGSARKYFSLHVCLFAPLWVFERTLSTYWAFYWLRLRGGYPFGERVLKKGIGRDWVEG
                     DRIASATSRK"
     gene            4771..>6079
                     /locus_tag="VFO30_08070"
     CDS             4771..>6079
                     /locus_tag="VFO30_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9419282.1"
                     /translation="MKDRAAKGPIRPDEQAELGDISPEEFRRQLYEIADWIADYHANI
                     ESLPVAPNAPPGAIQAALPAQPPEHGEPFERVFADFKRLIVPGLLHWTHPQFAAYFGT
                     TSNAAGIEGEILSAAVNVNAFTWRVSPAATELETVVIDWLRQWLGLPNEFGGVVYDTA
                     SIGIMHALAVAREEAASCTRKLGLTNRDLPTFSVYASDQAHSSVEKAAIALGLGEDNV
                     RRIPSDSAFRMQVSRLREMLTQDKAAGLQPLAVVATVGTTSTASIDPIPEIAKVCREE
                     KIWLHIDGAYGAGLALLPEHNWITAGWREADSLIINPHKMLFVPFDFSVLYVRDLERL
                     RRVFTLVPEYLRGDTVEAEKNYMDYGVQLGRRFRALKAWMVFRAFGRAGMAARIREHL
                     RIAHALADWIKADKRFELSAPVVMPVVCFRFVAGVGDAGPRSTI"
BASE COUNT         1238 a         1793 c         1752 g         1296 t
ORIGIN      
        1 aattttgaat cgcaaaacgt cgagctcgcc aagctcttca ccgacctcgg cgcgaaaccg
       61 ccggtctccg gcacagtcaa tgtgaagctc gacgcgcaag gcacgctcgc cgatctgagc
      121 gcgcggctcg acgtgcagat gcgcggtttg cgcagcgagc ggttgccgaa tttggagccg
      181 gccagcttcg atctcgtagc gcaatcacag catgatcagc tggcgatctc gggcaaattg
      241 cagcaagcga agatccagcc gctcgagatc accgcgaacc ttcccctgcg gctcgcgaaa
      301 actttgcgcg agcgaaagtt tgcagatgag acgccgctga gcgcaaaaat tcgcctgccg
      361 cgtagctcgg tcaattttgt gcggcaattc gtgcccgcgg tgcaggaact ggacggcgac
      421 gcggcgctcg atgtcgatgt tacgggaaca attggtcgcc cggtcttcac cggctccggt
      481 gacatcacga tcaacgtggc gcgcgcgagc gacgtcactc ttcccgcgct ccgggacttt
      541 aaagcccgac taaactttgc ccgcgatacg ctcagcctcg accaattcgg cggcgaactc
      601 tccggcggcc gctttaccat gaacggccgc gtcacgcttc caaagttgac cgcggcgaat
      661 ctcgatctgc aattgcgcgc cgacagcgcg ttgcttgcgc gcaacgacac attgactgca
      721 cgcgccgatg cggacatcaa aatcacgggg ccactcaata gtgcaaacgt caccggcacg
      781 gtggcgatga ccaacagtca tgtgctgaaa aatctcgacc tcctcccgat cggtttaccg
      841 gggcgcccgg caccgcagcc accgtcagcg cgtccggagt tttcgtttcc ggatccgccg
      901 ctgcgggatt ggaaatttga tgtggcgatt aaaaccaagg atccggtgct gattcgcggc
      961 tcgctcgcaa acggcggcgc gatcactgat gtgcatatcg gcggcaccgg cttgcatccc
     1021 gagctgcagg gcacggtgcg tttggagaac gtggaggcga cgctgccgtt tagtcgcttg
     1081 gagatttcgt ccgggttcgt ctatttcgac ccgaacgact cgttcaaccc gcggctggac
     1141 ctccacggca catccttgat ccatgaccac acgattcatg tttacattta cggcacggcg
     1201 ctcgctccgg aggcgatttt cacaagtgaa ccgccgttgc cacaggagga agtcatttcg
     1261 ctgctcgcca ccggtaccac acgcgaggaa ctaaccggaa gcaacaatgt gctggccggg
     1321 cgcgccgcga tgctcttagt gcagcaatta tatcgaaaag ttttcaagaa aggtcagccg
     1381 acgcagacca cttcagtttt cgatcggctg gatgtcgatc tgggcacggt cgatccgcgc
     1441 accggtcggc aacaggcgac ggcgcgtttc aaagtgaacg accaatttgt gctcgttggc
     1501 gatatcggcg taggcggcga ttatcgtggg atggtaaagt acctgatccg atttcgctga
     1561 tgcgttccct ttgtctgctc attgccgcat gcagcatcgg tctcgccggt aaacttgccg
     1621 cccaaaccgc ggtcgatgtc ccgcagtcgg aacggccgga agcaaagcaa aaacaacagc
     1681 aggcgattgc gaaacagcag gaaaaacacg cgcagcgcac cagcacgatc gagttccaag
     1741 gcgcaacggc tttcggcgag aaggagctgc gctcgcaatt gaaggagcaa atcacgacga
     1801 ttgaccagta cggactgaca cccgcgcgcg cggacgatct cgcgtttttt ctcgaggtct
     1861 tctaccgcaa gcacggttac gtgaaagtga acgtccatta tgtgatcgag tccggtgacc
     1921 ggctgcggct cttgatcaac gaggggacgc tgatgacgct gggtcgcgtt gtctttgaag
     1981 gcaacgtagg cgagccaaca gagaagctgt tcgatttcgc ggtcggtcca acacgagagc
     2041 gttactcaaa attgcaaaag gcgttgccct ttgtcgccgc cgatctggaa gaaggggccg
     2101 agctcgtcga tcggctttac gtcgcggaag gttatctcga cgccgtcgtg gacaagccgc
     2161 gctacgtttt ccatgacgag acaaatcagg tcgacgccat catttcgatt cacgagggtc
     2221 gacaatattt ttttggcgac gtctcattca gcggccggat gatttacgac ccagaagcgc
     2281 tgcgcggaca gattgtcgat cttttacagc aaccgtacac tgacgcgcgc gtcgccgata
     2341 ttccgcggcg gctgcaggcg tatttcaaat cgcgcggtta ctatgacgta aaagttgagg
     2401 cgacgggcga tccggaagcg gccgtgaagg ggcgtgtgcc tgtccaaatc gccatcgcag
     2461 ccggcccgct ttatcacttc ggcgacgtga ccgtgagtgg ccttagccgc ttgcggccga
     2521 gcttcgttac caggcgtttc cgcaatctgg ccggccagac ctacagcccg gaagtgctcg
     2581 atgaaaaatt ccgcacgctg atgcgcaccg ggctgttcaa tatcctccag atcaaacctg
     2641 tgccggtcga tgaaaacgag ctgcggctcg acatctccgc cgaggaagcc aagtctaagg
     2701 aattcggatt ctccgtcggc tacggcacgt tccaaggcgg catcgttggc gtgcaatacc
     2761 gcgaccgcga tctttttggc tatgggcggc cgctgacgac ttcgatcgaa ttgtcgcagc
     2821 gcggctacaa aggcgagata ctttacgagg acccattctt tttcgatacc gacctgagtt
     2881 tccggaacca gctggccgcg ctcacattcg attacgatgg ttactcgaag ttcgaagtcg
     2941 gcgatcgcat cgagttgacg cgcaaattca ccaagcaata tgaggctggt ttggttttcg
     3001 cggtgcggca cgtggagctt accagcgtct ctattaacca gcgctttctc ggccagactt
     3061 cctatttcat caacacgctc ggcttcaccc aaacgctcga cctgcgcgag agcccgctcg
     3121 tatcgccgcg cggcttggtc gtgaacacga ccctcgacgc ggcgacgggc gcgtttggca
     3181 gcgaaatcga tctcatccgc ggcacgacgc gggtggctta cttcctgccg tttggcccca
     3241 aaccgcttac gcccggcgtg gtcgaggaca gaacggaatc gtcgttacag cgttggtttc
     3301 atcaatcgct cctcgcactc ggggcgcggg cgggccttgc tcattcgctt aacaactccg
     3361 gccctgacga acccaccacc ttgccgattg acgaacgctt cttcaacggc ggcgccacca
     3421 cggtccgcag ctacggtgag cgcgacctcg ggccgcacga cccaaaaggc aatccgatcg
     3481 gcggcgaatt tttcactgtc ttcaatgtag aatacacctt ccccatcctc ggcgagttgc
     3541 agggcgcgct cttctttgat gcgggcgatc tcctgccgac ctcagaggaa cccggcgtcg
     3601 acgacatgca ctacgccctc ggcatgggtt tgcgttacaa attgcctatc ggcccgatcc
     3661 gacttgacta tggagtcaat cccgaccgcc aaccgggtga agacatcggc gcgtttcact
     3721 tcagcttcgg tttcgcattt tgaacgcgac ctatttgctg ccaatccgcc gcacaatttt
     3781 cgctgagact gaagccgccg acctgcgcga atattttgcg atgttagcgc atgcaggttg
     3841 tgcagtgatt gtcgtcgatg gatcgccgcc agaaattttt gcgcaacacc acgaattttg
     3901 gtcggacatt ggccgacacg aagtcgtgga cccgcgcttc aaatttttga acgacaaggt
     3961 caatggtgtt cataccggag ttgcactggc gactaacgac aaaatcatcc tcgccgacga
     4021 tgatattctc tacactgctg ctgacctcga gcagatctgc gctctccttg atcgatttga
     4081 agtcgttcgc ccacaaaatt acttcgtggc atcggcttcc agccgatgtc tttacgaaca
     4141 cgggcaagat gcccgtacca cttttccgcc ttgggcgaaa atggaatcgg cgcggatgct
     4201 gatcaatcgc gctacgttac gcgcggctga ttatcccagt acctgcgcat ttcgccgcca
     4261 agcattttta agtgccggtg agtatgacgg cgatgtcttg ttcgataacg aagaactcat
     4321 tcggcatttt gcgcgaagcg gcgcgcggat ttgttacgcg aacgatctgt tcatccgcaa
     4381 acgcccgcca acttttcgca aatggctcga acaacgtccg cgtcaggcct acgaagattt
     4441 tggattgcgg ttcaagacgg ttcttttcgc ctcgctgctg ccaatcggga ttgtgctcgc
     4501 ggtcagcgat gctcgacggt tgtcgatctt tctcgcgccg atcttcatcg ccttcgctgg
     4561 ctggctgcgc ggcagcgcgc gaaaatattt ttcgctccat gtttgtctgt tcgcgccgct
     4621 gtgggttttt gagcggacac tcagcaccta ctgggcgttt tactggctca ggttacgcgg
     4681 gggctatcca ttcggtgaac gcgtgctaaa aaagggaatt gggcgcgatt gggttgaagg
     4741 cgacagaatc gcatcggcga cttcccgcaa atgaaagatc gagccgccaa aggaccgatt
     4801 cgccctgacg aacaagcaga gctcggcgac atctcacccg aagaattccg ccgccaattg
     4861 tatgagatcg ccgactggat cgcggattat cacgcgaaca ttgagagctt gcccgttgcg
     4921 ccgaatgcgc caccgggtgc gatccaggcc gcattgcctg cgcaaccgcc ggagcacggc
     4981 gagccgtttg agcgagtctt cgctgacttc aagcgactga ttgtccccgg tttgctgcat
     5041 tggactcacc cgcagtttgc ggcttacttt ggcacgacca gtaacgcggc tgggatcgaa
     5101 ggcgaaattc tttccgcggc ggtgaatgtg aacgcgttca cgtggcgagt ctctccagct
     5161 gcgaccgaat tggagacagt cgtgatcgat tggttgcgac aatggctcgg gttacctaac
     5221 gaatttggcg gggtagtgta tgacacagca tcaatcggca tcatgcacgc cctcgctgtc
     5281 gcgcgggaag aggcggcgtc gtgcacgcgc aagctcggcc taaccaatcg agatttgccg
     5341 acctttagcg tctacgcttc cgaccaggcc cacagctcgg tcgaaaaggc ggcgatcgcg
     5401 ctcgggctgg gggaggacaa cgtgcgacgg atcccgagtg acagtgcgtt tcggatgcag
     5461 gtgtcgagac tgcgtgagat gctcacgcag gataaggctg ccggcttaca gccgttggca
     5521 gttgtggcaa cagtcggcac aacttccacc gcaagcatcg atccgattcc agagatcgcg
     5581 aaggtttgcc gcgaagagaa aatctggctg cacatcgacg gtgcttacgg cgccgggctc
     5641 gcgcttttac cggagcacaa ctggatcact gctggttggc gcgaagccga ctcactcatc
     5701 atcaatcccc acaagatgct cttcgtgccg ttcgatttta gcgtgcttta cgtccgcgat
     5761 ctcgagcggc tgcgccgcgt ctttacgctc gtgccagagt acctgcgcgg agacacagtc
     5821 gaggcggaga aaaattacat ggactatggc gtccagctcg gccgccgctt ccgcgcactc
     5881 aaagcttgga tggtattccg cgcgttcggc cgcgcaggca tggccgctcg gattcgcgaa
     5941 catctgcgga ttgcgcatgc cttagccgac tggatcaagg ctgacaagcg cttcgagctt
     6001 tccgcgccgg tcgtgatgcc ggttgtgtgt ttccgatttg tagccggcgt cggtgacgcc
     6061 ggaccgcgat caacgatcg
//
LOCUS       DASTDG010000245         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1178763, whole genome shotgun sequence.
ACCESSION   DASTDG010000245 DASTDG010000000
VERSION     DASTDG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178763"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFO30_08075"
     CDS             complement(<1..395)
                     /locus_tag="VFO30_08075"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419283.1"
                     /translation="MTRKSKRAREIYTLSDLQNWRAISRELQPPIRLGVFGDPVAHSL
                     SPKIQNAALKHHKIDVQYTAFQIASNELPDALRLVRELDFIGVNLTVPHKIAALADVD
                     DVDVAARKIGAVNTVAIRDGKLVGFNTDAV"
     gene            complement(578..1333)
                     /gene="lptB"
                     /locus_tag="VFO30_08080"
     CDS             complement(578..1333)
                     /gene="lptB"
                     /locus_tag="VFO30_08080"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET9419284.1"
                     /translation="MAAPRAPATKIDNVVSTEQLVKIYGGRAVVDGVNINARAGEIVG
                     LLGPNGAGKTTSFYMIVGLVRPNSGRVIFSGTDVTNYPMFKRARLGMGYLPQEESIFR
                     KMTVEQNIMAILETLSLSKYERRQRCEQLLHQFGIERIAKNQALTLSGGEKRRLTIAR
                     SLVTKPKLLMLDEPFSGVDPIAVYDVQQIIVNLRKSGLAILITDHNVRETLSIVDRAY
                     LIYEGRVESEGTKDFLINDPISRQLYLGERFKM"
     gene            complement(1363..2025)
                     /locus_tag="VFO30_08085"
     CDS             complement(1363..2025)
                     /locus_tag="VFO30_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET9419285.1"
                     /translation="MKDLVGLVLLIFAVTAGQLFAQAAVSPAQSFAAATTAKSAAQKE
                     KSPSNNLFGPATKGGEPTTTEIYANEAFFDSNKNMGIFTGSVKVIDPRFNLQSDKLTV
                     FISKGEKQGLEKAIAEGNVGLVRDRPDPNGGPPTRAVGRSEKATYIAATGDVELVGTP
                     RVQEGMNVHVATSPDTVMVINQNGQLKTHGPSRTDIRQQPTEEQKDQANASPSPSPSP
                     KQ"
     gene            complement(2022..>2418)
                     /locus_tag="VFO30_08090"
     CDS             complement(2022..>2418)
                     /locus_tag="VFO30_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419286.1"
                     /translation="AQSLTNIPLPVGHDAKGLVLPDFDSNGRLRGKFAAKTARRLDEG
                     HVGFEDLQIITYTPENRPDLQINMHTGVLDLKTRILSSKERTTVKRADFDIVGDSVQF
                     NTDQKTGHLIGNVKMVLNQQSQLIQKPGE"
BASE COUNT          463 a          673 c          704 g          578 t
ORIGIN      
        1 accgcgtcgg tgttgaagcc gacgagcttg ccgtcgcgaa tcgcgaccgt gttgaccgcg
       61 ccaatttttc gcgcagcgac atcgacgtcg tccacgtcag cgagcgcagc gattttgtgc
      121 ggcacggtta aattgacgcc gatgaaatcg agctcccgca ccaatctcag cgcgtccggc
      181 agttcgttag acgcgatctg aaaagccgtg tactgcacgt cgatcttgtg atgcttaagc
      241 gcagcattct ggattttggg cgaaagtgaa tgtgcgaccg ggtcgccaaa cacgccgaga
      301 cgaatcggcg gttgaagctc gcgcgagatc gcgcgccaat tttgcaagtc tgacagcgtg
      361 taaatctctc gtgcgcgctt gctctttcgt gtcatgtcga gtggagtcgc ctgccgcgcc
      421 gaagcttcat gcgtatgcgg gagacatctc ttactgttaa caatgaaaga ttgctcgagg
      481 tcgctcggaa ggacaaacaa tggcaaccga agcgcttgcc ctacaatttc caaaaacgcg
      541 cggcttttgt agggcgggcg catcgcctgc caaactgcta cattttaaag cgctcgccga
      601 gatacagctg tcggctgatg gggtcgttaa tcaggaaatc cttggtgccc tcgctttcca
      661 cccggccctc gtagatcagg taagcgcggt caacaatcga gagcgtctcg cgcacgttgt
      721 ggtcagtgat gaggatcgcc aggccggatt tgcgcaagtt gacgatgatc tgctgcacgt
      781 catacaccgc gatcggatcc acgccgctga acggctcgtc aagcatgagc agcttcggct
      841 tggtcacgag cgagcgcgca atggtgagcc ggcgtttttc gccgccgctc aaggtcagcg
      901 cctggttctt cgcgatccgc tcgataccga attgatggag caactgctcg caacgctggc
      961 ggcgttcgta ttttgaaagc gacagcgtct cgaggatcgc catgatgttt tgctcgacag
     1021 tcattttgcg gaagatcgat tcttcctgcg gcagataacc catgccgagt cgggcgcgct
     1081 tgaacatcgg gtagttcgtc acgtccgtgc cggagaaaat gacgcggccg ctgtttggcc
     1141 taacgagccc gacgatcatg taaaaactgg tcgttttgcc ggcgccattt gggccgagca
     1201 gaccgacaat ctcgcccgcg cgggcgttga tgttcacgcc atcaaccacg gcgcggccgc
     1261 cgtatatttt cacgagctgc tccgtcgaga caacgttgtc gatctttgtc gcaggcgctc
     1321 gtggtgcggc cacttccgtt ttcgatggtg cagtctggct gatcattgct tcggtgaagg
     1381 ggagggtgaa ggcgaggcgt tcgcctggtc cttctgttcc tcggtcggtt gctggcggat
     1441 gtcagtgcga ctcgggccat gagttttgag ctgcccgttt tgattgatca ccataaccgt
     1501 gtcagggctg gtcgcgacgt gcacattcat tccctcctgc acacgcggcg tgccgaccaa
     1561 ctcgacgtca cccgtcgcag cgatgtaggt ggctttttcg gaacggccaa ccgcgcgcgt
     1621 cggcggcccg ccatttggat ccggccggtc gcgcaccagg ccgacgttgc cttcggcgat
     1681 ggctttttcg agtccctgtt tttctccctt gctgatgaac acggtcaatt tatccgactg
     1741 cagattaaat cgcggatcga tcactttcac gctgccggtg aaaataccca tgtttttgtt
     1801 cgaatcgaaa aacgcttcgt tagcatagat ctcggtcgtg gttggctcgc cgccttttgt
     1861 cgccgggccg aacaaattgt tcgacggcga tttctccttc tgcgctgctg atttggcagt
     1921 tgttgcggcg gcaaacgatt gcgccgggga gacggcagcc tgggcgaaaa gttgcccggc
     1981 cgtcactgcg aaaatcagca ggaccaatcc gaccaagtcc ttcattcccc aggtttttgt
     2041 atcaattgcg actgctgatt gagcaccatt ttgacgttgc cgattaaatg gccggttttc
     2101 tggtccgtgt taaactgaac cgaatctccc acaatatcga aatcggcgcg tttgaccgtc
     2161 gtgcgttctt ttgaactaag gatgcgagtc ttcagatcga gcacgccggt atgcatgttg
     2221 atctgcaaat ccggtcggtt ttccggcgtg taggtgatga tctgcaaatc ctcgaagccg
     2281 acgtggcctt catcgaggcg gcgcgctgtt tttgccgcga attttccacg gagccggccg
     2341 ttggaatcga aatcgggcaa taccaggccc ttggcatcat gaccaacggg caacggaatg
     2401 ttggtgagac tttgcgca
//
LOCUS       DASTDG010000246         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_728769, whole genome shotgun sequence.
ACCESSION   DASTDG010000246 DASTDG010000000
VERSION     DASTDG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728769"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFO30_08095"
     CDS             <1..356
                     /locus_tag="VFO30_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419287.1"
                     /translation="VHSVFGQVSDRCFIDNIYGAPVSQATAAFVFIDSDGRLGTTTAN
                     SDPGPQRVQPQAIPQDAKQAILNRKVEKLQATVAQQQKQIEILTAQLKEQAAQIQKVS
                     ARLEVSKPAPEVAEN"
     gene            502..2022
                     /locus_tag="VFO30_08100"
     CDS             502..2022
                     /locus_tag="VFO30_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9419288.1"
                     /translation="MMRFLRKHRTWLMTVIAVLSIPFIFYFVRSDYSAIRPDEFARVY
                     GRTISLTEARRDARLFDLARVLGMTTFLQDLAGRAEGDPQGQVYGQFILNLQILRHEA
                     ARLGIRPAAADVIERVRNLEPFRSAAGFDMNKYNEFVENVLAPNGQTEAQLEEIVRDE
                     LALERIKQLVATGVTVSDAESKADFEKGYSKLFVSVVRLSAADFARDVKFTDDDIRKY
                     YEAHKTELKSPEKRKVDFVSLVLSDEQKKLTGKERIDALQKLADRANDFTQALLEKGA
                     DFKQVAAKFQLPVETTGEFTAAATDPQLKADAKLGSAAFRLAAQEPISDPIQVADGFY
                     VLHLAGIAEARPLSLEEANPQITEAIKSTRARELMMTKANEIAQQLRQALGSGQPLDA
                     AAQKTGVKLEKLPEFSLVEDSAKPEQQQTESPDLIMIKNAVAELEPGGVSDVFPSETG
                     ATIAVLERREPPEEKKYSENKSAFEQRYLRNMRTIAFHEWLRGRQRDAGLLPAQKS"
     gene            2041..2367
                     /locus_tag="VFO30_08105"
     CDS             2041..2367
                     /locus_tag="VFO30_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9419289.1"
                     /translation="MKPRDEIFDDANGDLAIGELESAVEKYRRCVELDPEFFDGWHAL
                     GMALMKVGRFNEAIEAGKKAVELRPNDQIGWTSLSLFYNRAGNIKEAEAAGAKAKILS
                     WGGKVK"
     gene            complement(2426..4606)
                     /locus_tag="VFO30_08110"
     CDS             complement(2426..4606)
                     /locus_tag="VFO30_08110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HET9419290.1"
                     /translation="MKKSLLAAVSIFSSVFVLYAQTEPQKSPVMEKSPAPGVHDARKQ
                     PAQTGPPPPILQGTAKSEAPSAKRPIETNAKPIDLSNFDTAVKPADDFFMYANGGWLK
                     RTEIPPEYSRWGSFNELIEKNNDALHTIAEKAANTPVDPKLAPETQKVGDYFTSGMDE
                     KTIESLKTQPLADEFKAIDAMKDRQDVLKEIAHLHSIGVNAFFEFGAGQDDKDSTREI
                     AQAVQGGLGMPDRDYYTKTDDASKKLRDQYVDHVTKMLTLLGDNNPGDEAKKILALET
                     KLAESSRTRVQLRDPIKNYNKMSVRDLQDLTPDWNWSNYFNDIELVAPGDINVHQPEF
                     FKAADAVFKSTSMDDWKAYLRWHLINAAAPELSKDFVDEDFNFKDRILHGTQQIKPRW
                     KRVIISEDGAIGEALGKLYVGFYFPPEAKARALELVNNLKAALADRIKTLEWMDEPTK
                     QEALRKLAAFTVKIGYPDKWLDYSLLTIDRGPFVLNAMRAAKFESTRDVNKIGKPVDR
                     TDWGMTPPTVNAYYNSNMNEIVFPAGILQPPFFYANADDAINYGGIGAVIGHEMTHGF
                     DDEGRQYDAVGNLRDWWSPQSAAEFKKRSQAVVNQYGEYEPLPGLHVNGELTQGENIA
                     DIGGVKLAYAALQKALDKNPQARQQKIDGFTPEQRFFLSFAAIWRQKSRDEDQKLRLT
                     VDPHSPARFRVIGPLSNLPEFQKAFGLPDGSPMVRAVDKRVNIW"
BASE COUNT         1031 a         1386 c         1366 g         1032 t
ORIGIN      
        1 gtgtgcacag tgtttttggc caggtaagtg acaggtgctt tatcgacaac atctacggcg
       61 cgccggttag ccaggcgact gccgcgttcg tattcatcga ttccgatgga aggttaggca
      121 cgaccactgc caactccgat ccgggtcccc aacgcgtcca accccaagcc attccccagg
      181 acgcgaagca agccatcctc aatcgcaaag ttgagaagtt gcaagcaacg gtcgcgcagc
      241 aacagaaaca aatagagatt ctcacagccc agctcaaaga gcaggccgcc caaatccaaa
      301 aagtgagcgc ccggctcgaa gtgagcaaac cggcgccgga agtggcggag aattaggtag
      361 cgcatgttag caattctgta gggggcgacg ttgtcacccc gtcggcaact taacggttag
      421 cggggggagg cgcctcgaca gagcgaggcg gctacagttt gcggctaagc agccccgcct
      481 ctacactgcc gcctcaggct tatgatgagg ttcctgcgca agcaccgcac ctggctgatg
      541 actgtcatcg ccgtcctctc gattccgttt atcttctatt ttgtccggtc ggattacagc
      601 gcgatccggc cggatgagtt cgcgcgcgtc tatggccgca ccatttcctt gacggaggcg
      661 cgccgggacg cgcgcttgtt cgacctcgcc cgtgtgctcg gaatgacgac gtttctgcaa
      721 gacctggccg gccgggcaga gggtgatccg caagggcagg tttacggcca gttcattttg
      781 aatctgcaga ttctgcggca tgaagccgcg cgactgggca ttcgcccggc cgcagccgat
      841 gtgatcgaac gggtgcgcaa tcttgagccg tttcgcagcg cggccgggtt cgacatgaat
      901 aagtacaatg aatttgtcga gaacgtgctc gcgccgaatg ggcaaaccga agcgcagctt
      961 gaggagatcg tgcgcgatga gctcgcactg gaaagaatca aacaactggt cgcaaccggt
     1021 gtgactgtgt cagacgccga gagtaaggcc gacttcgaga aaggttacag caaacttttc
     1081 gtcagcgtgg tacgcttgtc ggcggccgac ttcgccaggg atgtgaagtt caccgacgac
     1141 gacatccgca aatactacga ggcgcacaag acagagctga agagtcccga gaagcgcaaa
     1201 gtcgatttcg tgagtctcgt gctaagtgac gagcaaaaga agctcaccgg caaggagcgc
     1261 atcgacgctt tacagaaact ggccgatcgc gcgaatgatt tcactcaggc gctgctcgaa
     1321 aaaggcgccg acttcaaaca agtcgccgcg aagtttcaat tgcccgtcga aacgaccggt
     1381 gaattcaccg ccgccgcgac cgatccgcaa ttgaaggccg atgcaaaact tggcagtgcc
     1441 gcgtttcgtc tcgctgcgca ggagccaatc agcgacccga ttcaggtcgc cgacggattt
     1501 tacgtgctgc atctcgctgg cattgctgag gcgcgtccgc tctcactaga ggaagccaac
     1561 ccgcagatca ccgaggcgat caaatcaacg cgcgcccgcg agctaatgat gacaaaggcg
     1621 aacgagattg cgcaacagtt gcgacaagcg ctcggttcag gccagccgct cgacgccgcg
     1681 gcgcaaaaaa ccggcgtcaa actcgagaag ttgccggagt tttcgctcgt cgaggactca
     1741 gccaagccgg agcaacagca aaccgaatca ccggatctga tcatgatcaa aaacgccgtc
     1801 gccgagcttg aacccggagg agtgagcgat gtttttccct cggaaacagg cgcaaccatt
     1861 gccgtgctcg aacgacgcga gccgccggag gagaaaaaat attccgagaa caaatctgca
     1921 ttcgagcaac gatacctgcg caacatgcgc acgatcgcct ttcacgaatg gctgcgcggg
     1981 cggcagcgtg acgccggtct gcttcccgcg cagaagagct aagatcgaca atcaaccaga
     2041 gtgaagccgc gcgacgagat ttttgacgac gcgaatggcg atctcgccat cggggagttg
     2101 gaaagcgcag tcgagaaata tcggcgctgt gtcgagctcg atccggaatt tttcgacggc
     2161 tggcacgcac ttggcatggc tttgatgaag gtcggccggt tcaacgaagc gatcgaggcc
     2221 gggaagaaag cggtggagct tcggcctaac gaccagatcg gctggacgag cttgtcgttg
     2281 ttttacaatc gcgccggcaa cattaaagaa gccgaagccg ccggtgcgaa agccaagatt
     2341 ctgtcctggg gcggtaaagt aaagtagcgc aagcctccgg cttgcgacct gaaaaattgc
     2401 aagcgggacg catgcgctac ttatctcacc agatattcac gcgcttatcg acggcgcgaa
     2461 ccattggtga gccatctgga agtccgaacg ccttttgaaa ctccggcaaa ttggagagtg
     2521 gcccaatcac gcgaaaacgt gccggcgaat gcggatcaac agtaagcctc agcttctgat
     2581 cttcgtcgcg gctcttctgg cgccagatgg ccgcaaacga gaggaagaaa cgctgctcgg
     2641 gtgtgaagcc gtcgatcttt tgctggcgcg cctgtggatt tttgtcgagc gccttttgca
     2701 gcgcggcgta ggcgagtttc accccgccga tatcggcgat attttcgcct tgagtcagct
     2761 cgccattgac gtgcaggccg ggcagcggct cgtattcgcc gtattggttt accaccgcct
     2821 gggaacgttt cttaaactca gccgcggatt gcggcgacca ccagtcacgc aaatttccca
     2881 cggcgtcgta ttgccggccc tcgtcgtcaa agccatgcgt catttcgtga ccgatcaccg
     2941 cgccgatccc gccgtagttg atggcgtcgt cggcgttcgc gtagaaaaac ggcggctgca
     3001 aaatcccggc cgggaaaaca atttcgttca tgtttgaatt gtaataggcg ttcaccgtcg
     3061 gcggagtcat tccccagtcc gtgcgatcga ccggtttgcc gatcttgttc acgtcgcgcg
     3121 tcgactcgaa cttggccgcg cgcatcgcgt ttaagacgaa cggcccgcga tcgatcgtca
     3181 gcagtgagta atcgagccac ttgtcgggat aaccgatctt caccgtgaac gcggccagct
     3241 tcctgagcgc ttcctgtttg gtcggctcat ccatccactc cagcgtcttg attcgatcgg
     3301 cgagcgcggc ttttagattg ttcaccagct cgagcgcgcg cgccttggct tcgggtggaa
     3361 aataaaaacc tacatacaat ttgccgagcg cctcgccgat ggcgccatcc tcggaaatga
     3421 tcactcgttt ccagcgtggc ttgatttgtt gggtgccgtg cagaatccga tctttgaagt
     3481 tgaaatcttc gtcgacgaaa tctttggaaa gctccggcgc ggccgcgttg atcagatgcc
     3541 agcgcagata cgctttccaa tcgtccatcg acgtgctctt gaacaccgcg tcggccgctt
     3601 tgaaaaactc cggctgatgc acgttgatat cgcccggcgc gaccagctcg atgtcattaa
     3661 aataattgct ccagttccag tccggcgtca ggtcctgcaa gtcccgcaca gacatcttat
     3721 tgtagttctt tattgggtcg cgcagttgga cgcgcgtgcg cgatgattcc gccagcttcg
     3781 tttcgagcgc gagaatcttc ttcgcttcgt cgcccgggtt gttgtcgccg agcaacgtca
     3841 gcatcttcgt tacatgatcg acatattgat cgcgcagctt tttcgaagcg tcgtccgtct
     3901 tggtgtaata atcgcggtcc ggcatcccga gcccaccctg caccgcctgt gcgatctcgc
     3961 gcgtgctgtc cttgtcatcc tgcccggccc caaactcgaa gaacgcattc acaccaatcg
     4021 agtgcaggtg cgcgatctcc ttgagcacat cttgtcgatc tttcatcgcg tcgattgcct
     4081 tgaactcgtc ggctaacggc tgcgtcttta gcgactcgat cgttttttca tccatgccgc
     4141 tcgtgaaata atcgccgacc ttttgcgttt ccggggcaag ctttgggtca accggcgtgt
     4201 tcgccgcctt ttccgcgatc gtgtgcagcg cgtcgttgtt cttctcgatc agctcgttga
     4261 agctgcccca gcgcgagtat tccggcggga tctccgttcg cttgagccag ccgccattgg
     4321 cgtacatgaa aaaatcgtcg gccggtttca ctgccgtatc gaaatttgag agatcgatcg
     4381 gcttcgcgtt cgtttcaatc ggccgcttcg cgcttggcgc ttcgctcttc gcagtacctt
     4441 gtagaattgg tggcggtgga cccgtctgtg ccggctgctt tctcgcgtca tgcacgcccg
     4501 gcgccggcga tttttccata accggcgatt tctgcggttc ggtctgcgcg tagagaacga
     4561 acacgctgga gaaaatagaa acggcggcga gaagggactt tttcatcgcg caatcaaccg
     4621 aaaaccagcc ggcttgtcca atctgcaaga tcgacaactc cttcacacaa agtccaccaa
     4681 ggacacgaag attgttgtag aggcgcttgt accaagcgcc tgtctttttg aattcggtgc
     4741 ttgtcaacaa tcatgccgct ttgcggcaca ctacaagcct ggttctttat ggatgcacta
     4801 gcgggagcgt aagca
//
LOCUS       DASTDG010000247         7168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_562722, whole genome shotgun sequence.
ACCESSION   DASTDG010000247 DASTDG010000000
VERSION     DASTDG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562722"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..865)
                     /locus_tag="VFO30_08115"
     CDS             complement(122..865)
                     /locus_tag="VFO30_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9419291.1"
                     /translation="MSALVQLVDLSKRYGEAPALHPTNLAIERGKTTVLIGPSGCGKS
                     TLLRLIIGLTNPDNGAIFFDRQPVTPANIDMVRRRTGYVIQEGGLFPHLTARANVLLM
                     PRHLAKPEEESRARLRELCALTRFPENLLQRYPLELSGGQRQRVSLMRALVLSPELLL
                     LDEPLGALDPLVRASLQKDLKQIFARLHQTALLVTHDLAEAAYLGDEVVLMNEGRIVQ
                     QGSIVDLRERPANEFVTEFINAQRSLAVL"
     gene            complement(872..1837)
                     /gene="egtD"
                     /locus_tag="VFO30_08120"
     CDS             complement(872..1837)
                     /gene="egtD"
                     /locus_tag="VFO30_08120"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET9419292.1"
                     /translation="MTAPTNAVVDLSAKRHFRDDVIAGLTNSPRTLPCKYFYDHRGAE
                     LFQKICELPEYYITRTEIGILRLRSAEIATALGPDIELIGLGTGAGTKTRILLEELQT
                     PRVYIPIDISKEQLEKSSARFRQRFPDLQILPFCADYLEPFELPLPRQLAARSVVYFP
                     GSTIGNFEPNAAVSFLERLVDLVGDGGGLLIGVDLQKDSHVIEAAYNDAAGVTAAFNL
                     NLLARINRELDADFDLKSWEHRANYNKTLGRVEMYLVSRTKQRVRIETQHFSFARGEA
                     ILTEYSYKYTPPGFAALARKAGFRFENLWSDKAQFFGVFYFRIPE"
     gene            complement(1834..3174)
                     /gene="egtB"
                     /locus_tag="VFO30_08125"
     CDS             complement(1834..3174)
                     /gene="egtB"
                     /locus_tag="VFO30_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET9419293.1"
                     /translation="MKGATVQTHATRGASAADELLARFHEVRDFTLGLTSGLAPEDCV
                     VQSMPDVSPTKWHLAHTTWFFETFVLKQWLPGYRSQIPEYAYLFNSYYNAAGDMHRRD
                     LRGLIARPTVAQAHRYRALIDEEIDRLLSSADDRLFLEIEPVLTLGIHHEQQHQELLL
                     TDIKHVFAQNPLFPVYRERKIDNREAAHVPQQFIEFDESTIAIGHDGDGFAYDNESPR
                     HRALILPFALANRPVTNAEFLEFIEDNGYSRPEFWLSLGWATINEQRWRAPLYWMERD
                     GEWWNFTLSGLRQINPDEPVTHISYFEADAYANWAGARLATEFEWERAAADVPVDGNF
                     VESETFHPQPWHRHPADDHGLEAHAMKQDRHLHQIFGDVWEWTRSAYSPYPGYRAAPG
                     ALGEYNGKFMCNQYVLRGGSCATSRTHIRKTYRNFFQPEKRWQFTGIRLARDLS"
     gene            complement(3198..3596)
                     /locus_tag="VFO30_08130"
     CDS             complement(3198..3596)
                     /locus_tag="VFO30_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419294.1"
                     /translation="MNEYSSEEPGKISLHGNGMGVPSPDDIERRAREIALIDERNPDE
                     FTDADWNQARKELMGVENNTPPEETPDTAEVTEEWNVVPGSKGHRAHRAGDDDEALGA
                     HLVSDGLEEAAHDQMLEARREELEEEGGIT"
     gene            3712..4593
                     /locus_tag="VFO30_08135"
     CDS             3712..4593
                     /locus_tag="VFO30_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9419295.1"
                     /translation="MKLQPHIVPLKGGGHLAYSEYGDANGTPVLFCHGWPSSRTMAEL
                     TDAAAHELAVRIISPDRPGISASAFVANRKLIDWPPLVRELMDVLAIREFRILAISGG
                     APYAYATGWQMPERVRAIAIVSGAAPILDLADESGLLRLYRWMLALYRKHPHLLRTMF
                     YLARPIASLRPPVRFRPLLLRLLKLQPCDAAALRDSAAFEACFESQRRAWRASAKGIM
                     TDAEIYANAWGFRLEDVNVPVRLWHGKADRAFSIEVARKVAKRLPNCSGRYIDNEGHY
                     SLPIRHIREILADLIAV"
     gene            4644..5717
                     /locus_tag="VFO30_08140"
     CDS             4644..5717
                     /locus_tag="VFO30_08140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET9419296.1"
                     /translation="MEDFTAGAIHDQPVRRQWSVEPPFDERVRELVRDWGAEKSPELI
                     EEMIVTALKMARDDMSVADLKLINRSVKEMRYAAKVFAPFQNVRKVVVFGSARILPEA
                     PECKLAEEFARAMVAQNYMVITGGGEGIMGAAQRGAGREKSFGLNIRLPFEQRPNETI
                     NGDPKLINFNYFFTRKLNFVKETHAFALFPGGLGTMDETFEVLTLMQTGKARVIPVVL
                     IDRPDGGYWETWLKFLTNHLFRIGFVSPEDFHFFKIFHSVEAATQEILQFYRVYHSAR
                     WVGEQLIIRVSQRLSSKAIVDLNKEFADLVRSGEITQGTALRQEKNEPDIWHLPRLLL
                     TPYRHSFGRFRQLIDAINRSSLV"
     gene            complement(5706..6884)
                     /locus_tag="VFO30_08145"
     CDS             complement(5706..6884)
                     /locus_tag="VFO30_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9419297.1"
                     /translation="MNVLYMESSRSWGGQEYRTCLEVNWLNAHGHEAWLICDPGSEVI
                     VKARELGTRVIALPLRRRLDPLTWWRLWRLAARLHIDLIKTFSSKDHWLALPIYFSGV
                     PLIRARCITDPIGSKWRAFIFKHGCSKILADAGVIKRQLIDQNGIDPAKIEVVGSAVD
                     LQKFNPSRDRMKFRRELGLTADTLLIANIGMVRPDKGQTVFVEAAGRVLEQRPDARFV
                     VVGQGTGSLKLGAKVRLAISRAGLSGRVFAVGYRWDTPDILAASDMVVIASLHTEASP
                     IVLREAFASGRPVVATKVGDIPEIIAHGQNGLLVEPGDSRALATAILAFLSDGALARR
                     CSANGLRYAREHFAFDQMMKSKLRIDTELVNRARKASPRDQTESKSTPVAATEAASHE
                     "
     gene            complement(6929..>7168)
                     /locus_tag="VFO30_08150"
     CDS             complement(6929..>7168)
                     /locus_tag="VFO30_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9419298.1"
                     /translation="NLMLQTTPDEIGDDLPLFGPNGLGLDSIDALELVVSLEKTFGVA
                     VPNSEVAGKALRTVNTIHDYILEKRAATAQINPTA"
BASE COUNT         1441 a         2039 c         2149 g         1539 t
ORIGIN      
        1 tatgattcgg tgaatttctt cgagccgatg gccaccggct ccgctgcgga agcggcaaaa
       61 caaatcagga aagctggaac gcagcaaaag gtgataagaa aatctgcttt cctgattctc
      121 gtcataaaac agcaagactg cgttgcgcat tgatgaattc ggtcacgaac tcgttcgccg
      181 ggcgctcacg aagatcgaca atggaccctt gctgcacgat tcgcccttca ttcatgagca
      241 cgacttcatc gccgagatac gccgcctcgg cgagatcatg cgtgacgagc agtgccgttt
      301 ggtgcaggcg tgcaaaaatc tgcttcagat ctttttgcaa agacgcgcgc acgagcgggt
      361 cgagtgcgcc gagtggttcg tcgagcagga ggagctcggg cgagagcacg agcgcgcgca
      421 tcaagctgac gcgctggcgt tggccgcccg agagctcgag cgggtagcgc tgaagcaggt
      481 tttccggaaa gcgagtaagc gcgcaaagtt cgcgcaaacg ggcccgcgac tcctcttctg
      541 gtttagcgag atgtcgcggc atcaacagca cattcgcgcg cgcagtcaga tgcggaaaca
      601 aaccgccttc ctgaatgacg tagccggtgc ggcggcggac catgtcgatg ttggcgggcg
      661 tgaccggttg gcggtcgaag aaaattgcgc cgttgtccgg atttgttagg ccgatgatga
      721 ggcgcaggag cgtcgatttg ccgcaaccgc ttggaccaat gagcaccgtt gttttccccc
      781 gctcaatcgc caggttcgtc ggatgcaatg ccggagcctc gccgtatcgc ttgctcagat
      841 cgaccagttg gacgagcgcg ctcacgcgcg gctactctgg aatgcgaaaa tagaagacgc
      901 cgaagaactg cgctttatcg ctccagaggt tctcgaagcg gaaaccagcc ttgcgcgcga
      961 gcgccgcaaa acctggtggc gtgtatttgt aggaatactc agtcaggatt gcttcgccgc
     1021 gggcgaaact gaaatgttga gtttcaatcc gcactcgctg ttttgttcgg ctcacaagat
     1081 acatttcgac gcggcctaat gttttgttgt agttggcgcg gtgttcccaa cttttaagat
     1141 cgaaatcagc gtccagctcc cgatttatcc gcgcgagcag gttaaggttg aatgctgccg
     1201 tcacgccggc ggcatcattg taagcggctt cgattacatg actatctttc tggagatcga
     1261 cgccaatgag aagcccgccg ccgtcaccga caagatcgac aagtcgctcc agaaaactaa
     1321 ccgccgcgtt cggttcgaag ttgccgatag ttgatccggg gaaataaacc acgctgcgtg
     1381 cggcgagctg gcgtggaagc ggcaactcaa acggctccag gtagtcagcg caaaacggca
     1441 ggatctgcag atcgggaaat ctttgccgaa atcgcgcgga tgatttttcc agttgttctt
     1501 tcgagatatc gattgggatg tagacccgcg gagtttgcag ttcttcaagc aaaattcggg
     1561 tcttagttcc cgctccagtg ccgaggccga tcagctcaat atcgggcccg agggcggtgg
     1621 cgatctccgc gctgcgtaag cgcaatatgc caatttccgt ccgggtaatg taatactcgg
     1681 gcagttcgca aattttctgg aacaactcag cgccgcgatg gtcgtagaaa tacttgcacg
     1741 gcaacgttcg tggggagttc gtcaggccgg caatgacgtc atcgcggaag tgccgcttcg
     1801 ccgaaagatc gacgactgcg tttgtcggag ccgtcacgac agatcgcgtg ccaaacgaat
     1861 tcccgtgaat tgccaccgtt tctcgggttg aaagaaattg cgatacgttt tgcggatgtg
     1921 tgtgcgcgac gtcgcgcaag agccgccgcg cagcacgtac tgattgcaca tgaacttgcc
     1981 attgtattcg cccagcgctc cgggcgcggc gcgatagcct ggatagggcg aataggcgct
     2041 gcgtgtccac tcccaaacgt cgccgaagat ttggtgcaga tgtcgatctt gcttcatggc
     2101 atgggcttcc agcccatgat catcggcagg atgccgatgc cacggctgtg ggtggaatgt
     2161 ttcgctttcg acaaagttgc cgtcgaccgg aacgtccgcc gcagcccgtt cccactcgaa
     2221 ttccgtcgcc aaacgcgcac cggcccagtt cgcatacgca tcggcttcga aataactgat
     2281 gtgcgtcacg ggttcgtcag gattgatttg ccgcagacct gacaaagtga aattccacca
     2341 ttcgccgtcg cgttccatcc agtagagcgg cgcgcgccaa cgttgctcgt taatggtcgc
     2401 ccagcctaac gagagccaaa attcgggacg cgaatacccg ttgtcctcta tgaactcgag
     2461 gaactcggcg tttgtcactg ggcggtttgc gagtgcgaac ggcagaatga gcgcgcgatg
     2521 ccgcgggctt tcattgtcat aggcaaaacc gtcgccgtcg tgtccgatcg caatggtgga
     2581 ttcgtcaaat tcgataaact gctgcggcac gtgagcggct tcgcgattgt cgatcttgcg
     2641 ctcgcgatac accgggaaca gcgggttttg cgcgaacacg tgcttgatgt cggtgagcag
     2701 taattcctga tgctgctgct catgatgaat gccgagcgtc aggaccggct cgatttcgag
     2761 gaacaatctg tcatctgcgc tggaaagtag gcgatcgatc tcctcgtcga tcaatgcacg
     2821 atagcggtgc gcttgtgcga cagttggccg cgcgatcaag ccgcggaggt cgcggcggtg
     2881 catgtcgccg gcggcattat agtaggaatt aaacaggtaa gcgtattcgg gaatttgcga
     2941 acgatagccg gggagccact gtttgagcac gaacgtctcg aaaaaccagg tcgtgtgggc
     3001 gagatgccat ttcgtcgggc tcacgtcggg cattgattgc acgacgcaat cttccggcgc
     3061 gaggccgctt gttaggccaa gtgtgaagtc gcggacctcg tgaaaccgcg cgagcaactc
     3121 gtcggccgcg ctggcgccgc gcgtcgcgtg cgtttgcacg gtcgcacctt tcatccgagt
     3181 cgcgaccgcg ctttggctca agtaatgccg ccttcctctt ccagctcttc gcgtcgcgct
     3241 tccagcatct ggtcgtgcgc cgcttcctcc aagccgtcgc tcaccagatg cgcgccgagc
     3301 gcttcgtcat catccccggc gcgatgcgcg cggtgaccct tggagcccgg taccacattc
     3361 cattcttcgg tcacttcagc cgtgtccggc gtttcctccg gcggggtgtt attttccacg
     3421 cccatcagtt ctttgcgcgc ctggttccag tcggcatcgg taaattcgtc gggattgcgc
     3481 tcgtcgataa gcgcgatctc gcgcgcgcgg cgttcaatgt catcgggcga agggacgccc
     3541 attccattac cgtggagcga aatcttgcca ggttcttctg aggagtactc gttcatacaa
     3601 attaaatacg caggatcggc ggccaatggc ctcctttcac gctgcgaaca aagtatgctg
     3661 ggggccgaga cagttgtcca gtgttagcga gttcaggaac gcagtcgggc tatgaaactg
     3721 caaccgcaca tcgtgccgct caaaggcggc gggcacctcg cctacagcga atatggcgac
     3781 gcgaacggca cgccggtgtt gttttgtcac ggctggccga gctcgcgcac gatggccgag
     3841 ttgactgatg cggccgcgca cgaacttgcg gttcgcatca tctcgccaga tcgaccggga
     3901 atcagtgcgt cggccttcgt cgcaaatcga aagctgatcg actggccgcc gcttgtacgc
     3961 gagctcatgg atgtgctcgc gattcgcgag tttcgcatcc tggcaatttc cggcggcgcg
     4021 ccttatgcgt acgcgacggg gtggcaaatg ccggagcggg tgcgcgcgat cgcaatcgtg
     4081 agcggtgcgg cgccgattct cgatcttgcg gatgagagtg gattgctccg cctgtaccgc
     4141 tggatgctgg cgctttaccg caagcatccg catcttctgc gcacgatgtt ttatctcgcg
     4201 cggcccatcg cgtccttgcg tccccccgtt cgatttcggc cgttgctttt gcgattgctc
     4261 aagctgcagc cgtgcgatgc cgcggcactg cgcgactcgg ccgcgtttga ggcgtgtttt
     4321 gaaagtcagc gccgcgcctg gcgcgcttcc gcgaaaggga ttatgaccga cgcggaaatt
     4381 tacgccaacg cgtggggctt ccgtttggaa gacgtcaatg tgccggtgcg gctctggcac
     4441 ggcaaagcgg accgcgcgtt ctcgatcgag gttgcaagga aggtggcgaa acgattgcca
     4501 aactgctcgg ggcgctacat cgacaatgag ggacattact cgctgccgat ccggcacatc
     4561 cgcgaaatcc tcgccgatct catcgcggtc tgaacggaaa ctttcgccgt gcgcttggca
     4621 taactcgcgc taggaatacg cgggtggaag atttcacagc gggagccatt cacgatcagc
     4681 cggtgcgccg acaatggagc gtcgagccgc cgtttgacga gcgcgtccgc gagctggtgc
     4741 gcgactgggg cgcggagaaa tcgccggagc tgatcgagga gatgatcgtg acggcgctga
     4801 agatggcgcg cgacgacatg tcagtggccg atctgaagtt gatcaaccgc tcggtcaagg
     4861 agatgcgcta cgcggcgaag gtgttcgccc cgttccagaa cgtccgcaaa gtggtcgtgt
     4921 tcggttcggc gcggattttg ccggaggcgc cggagtgcaa actggcggag gagtttgcgc
     4981 gcgccatggt ggcgcagaat tacatggtca ttaccggcgg gggcgaaggg atcatgggcg
     5041 cggcacagcg cggcgccggc cgcgaaaaaa gtttcggcct gaatattcgg ctgccgttcg
     5101 agcagcggcc taacgaaact atcaatggcg atccgaagct gattaatttc aactacttct
     5161 tcacgcgcaa gctcaacttc gtgaaggaga cgcatgcttt tgcgctattc cccggcggac
     5221 tcggcaccat ggatgagaca ttcgaggtgc tcacgcttat gcaaacgggc aaagcgcgcg
     5281 ttattcccgt ggtgctcatc gaccgccctg atggaggtta ctgggagacg tggctgaaat
     5341 ttttaaccaa tcacctgttc agaatcggct tcgtctcgcc ggaggatttt cacttcttca
     5401 agatcttcca cagcgtggag gcggcgactc aggagattct gcaattctac cgggtctatc
     5461 actcagcgcg ctgggtgggc gagcagctta tcattcgcgt ctcacagcgg ctgtcgtcga
     5521 aggcgattgt cgatcttaac aaagaattcg ctgatctggt gcgcagcggc gaaattaccc
     5581 aagggactgc attgcgtcag gaaaagaacg agccggacat ttggcatttg ccgcgcttgc
     5641 ttctgacgcc ataccgccac agcttcggcc gctttcggca attgattgac gcaatcaatc
     5701 gctcgtcact cgtgtgacgc cgcctccgtc gcggccactg gcgtactctt gctctccgtc
     5761 tggtcccgag gactggcctt ccgcgcccga ttaaccaact ccgtgtcgat gcgcagtttt
     5821 gatttcatca tttggtcaaa cgcgaagtgc tcacgtgcgt aacgcagtcc attcgctgag
     5881 catcgccggg ccagagctcc atcgctgaga aacgcgagaa ttgcagtggc gagtgcgcgg
     5941 ctgtcacccg gctccaccaa caatccattt tgcccatgtg cgatgatttc cgggatgtcg
     6001 ccgacttttg tcgcaacgac gggtcggccg ctggcaaagg cttcgcgcaa cacgattggc
     6061 gatgcttcgg tgtgcagaga cgcaatcacc accatgtcgg atgcggcgag gatatctggc
     6121 gtgtcccacc ggtaaccgac cgcaaagacg cgcccgctca gcccggcgcg actaatcgcc
     6181 agccttactt ttgcgccgag cttcagcgac ccagtgcctt gacctacaac aacaaaacgc
     6241 gcatccggcc gctgttctag aacgcgccca gctgcttcga caaaaaccgt ctgccccttg
     6301 tccggccgaa ccatgccgat gttcgcgata agcagtgtgt ccgccgttaa accgagttcg
     6361 cgtcggaatt tcatgcggtc gcgtgatgga ttgaatttct gtaggtcaac cgctgagccg
     6421 acgacctcga ttttagccgg gtcgataccg ttctggtcga taagctgacg tttaatcaca
     6481 cctgcatcgg cgagaatttt cgagcacccg tgcttgaaaa tgaaagcgcg ccatttacta
     6541 ccgatcggat cggtaatgca acgcgcgcga ataagcggaa cgccggaaaa gtaaatcggc
     6601 agcgcgagcc agtggtcttt ggagctgaat gtcttgatca agtcgatgtg tagccgcgct
     6661 gcgagtctcc agagtcgcca ccaagtaagt ggatcaaggc gtcggcgcag cggcagggcg
     6721 ataacacgcg tacccagctc gcgcgccttg acgattactt ccgaacccgg atcgcagatc
     6781 aaccaggctt cgtgaccgtg agcgttgagc caatttactt cgagacaggt gcggtattcc
     6841 tggccgcccc agctgcgact gctctccatg tagagaacgt tcatctaaga tcgacaatca
     6901 ctcggggcga tgccgccagc agcccgggtc aagccgtcgg gttgatttga gccgtcgccg
     6961 cgcgtttttc gagaatgtaa tcgtggattg tgttgacggt gcgcagcgct ttgcctgcga
     7021 cttcggaatt tggcaccgcg acaccgaaag ttttttccag gctcacaacc agctccagcg
     7081 cgtcgatcga atccaagccg agcccgttcg gcccaaagag cggcagatcg tcgccgatct
     7141 cgtcgggcgt ggtttggagc atcaggtt
//
LOCUS       DASTDG010000248         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1415455, whole genome shotgun sequence.
ACCESSION   DASTDG010000248 DASTDG010000000
VERSION     DASTDG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415455"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="VFO30_08155"
     CDS             <1..2115
                     /locus_tag="VFO30_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9419299.1"
                     /translation="PALFPLLGVEPIKGRVFTQEEGGQGRDDVVIISARLWERRFNSD
                     PTIVGNKISLNGRPHTVVGIMPRKFDFPLPLFNVQGSRFASRPDIWKPIAFTNDELSA
                     RYWRSYGVIGRLRRGVSNAQAQAQIDTITANFRQTNPKNYPAGLTEFGAKLYPLRDQV
                     VGPMRTGLWILLGAVTLVLLIACANLTIMLLARAASREREMAIRVALGASLKRMLRQL
                     LTESVLVSILGGTAGVLLAIWAIDLLRMIGAQTVPRLREVNLDLSVLAMTFGVAVVTG
                     ILFGLVPAIVSAKPELTEALKEGGRGSTGGAHRNRLRNALIVAETVLALVLLIGAGSL
                     VKSFVRLQNVSPGFNPHNVLTAEISLPVEKYPRDPRGLAVINFYNEAERRIKNIPGVQ
                     HAAFTVMLPLSGSNTDSSFIIEGQDPKMTGAYPDDEIRDITPDYFRVLETPLLKGRFF
                     TEADTADSPQVVIVNQAFVKKYFRDGEALGKRISRDDTDPKWATIVGVVADIKHRGLD
                     TEAQPEYYLPHTQGPDREMVLAVRSAQDARTLAHTVQEELRNVDADIPLSNVRTLDAV
                     VADSIAPRRLSVVLLSAFSGIALLLASVGMYGVMSFLVVQRTHEIGVRMALGAQRSDV
                     LRMVIGHAAKLLGIGAAIGLPIAFFSNSALRTLLYQVGPFDLSIFLIVTLILAGVALL
                     ASYIPAVRATRADPMLALAHNA"
     gene            2122..>2509
                     /locus_tag="VFO30_08160"
     CDS             2122..>2509
                     /locus_tag="VFO30_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9419300.1"
                     /translation="MSTLLHDIRYGVRMLLKHKGFTAVAVIALGLGIGANTAIFSLVN
                     GVLLRPLPFPDAERIIYIEGKNPAAAGITESNISFLDFTDWSQQTDLFASTAAYWTGT
                     ANFGADGAEPERVPRAGVTTGFFSVLG"
BASE COUNT          493 a          723 c          734 g          559 t
ORIGIN      
        1 ccggctctgt tcccactact cggcgtggag cccatcaaag gtcgcgtttt cactcaagag
       61 gagggcggtc aaggtcgcga cgatgtcgtt atcatcagcg cgcgattgtg ggagcgccgc
      121 ttcaactccg atccgacaat cgttggcaac aagatctcat tgaacggtcg gccgcatacg
      181 gtcgtgggaa tcatgccgcg gaaattcgat tttccgttgc cgctgttcaa cgttcaggga
      241 tcgcgcttcg ccagtcgccc cgatatttgg aagccaattg cgtttacaaa cgacgaattg
      301 tcggcacgct attggcggag ctatggcgta atcgggcggc tgcgccgagg tgtttccaac
      361 gcccaagctc aagctcaaat cgacaccatc acggcaaact tccggcagac gaatcccaaa
      421 aattatcctg ctggactcac cgagttcggc gcaaagcttt acccgttacg ggatcaggtc
      481 gtcggcccga tgcgaacggg tctttggatt cttctcggcg cggtgacact cgttttgctg
      541 atcgcgtgcg caaatctcac cattatgtta ctcgcgcgcg cggcatcgag agagcgagag
      601 atggcaattc gcgtcgcgct gggcgcgagc ctgaagcgca tgctgcgaca attgctgacc
      661 gaaagcgtgt tagtgtcgat cttgggtggg accgctgggg tgctgctcgc aatctgggca
      721 atcgacctct tgcggatgat cggcgcgcaa accgtgccgc ggcttcgcga ggtgaatctc
      781 gatctgagcg tgctcgcgat gacgttcggt gtggcggttg taaccggaat tcttttcggc
      841 ctcgtgcccg cgatcgtcag cgcaaaaccc gaattgaccg aagcgctgaa agaaggtgga
      901 cgggggtcga ctggcggcgc ccatcgtaac cgcctgcgca acgctctgat cgttgccgaa
      961 actgttctcg cgctcgttct gctgatcggc gccggctcgc tcgtgaagag ttttgtccga
     1021 ctgcaaaatg tttctcccgg atttaatcca cacaatgtgt tgacggcgga gatttctttg
     1081 ccggtggaaa aatatccgcg tgatccgcgt ggtctggcag tcattaattt ctacaacgaa
     1141 gcggagcgtc ggatcaaaaa catcccgggc gttcagcatg ccgcgtttac ggtgatgctg
     1201 ccattaagcg gatcgaatac ggacagctca ttcatcatag agggtcagga tccaaaaatg
     1261 accggcgcct accccgacga cgagattcgt gatatcaccc cggattattt tcgggtgttg
     1321 gagacaccgt tgttgaaagg gcggtttttc accgaggctg acacggccga ttcgccgcag
     1381 gtggtgatcg tgaaccaggc gttcgtcaaa aaatacttcc gtgacggcga agctctcggt
     1441 aagcgcatct cacgggacga cacagacccg aaatgggcga caattgttgg agttgtagcc
     1501 gacatcaagc atcgcggtct ggatacggaa gcgcagccag agtactacct gccgcacaca
     1561 cagggacccg accgggagat ggtactggcg gtgcgcagtg cacaagacgc gcgcacgctg
     1621 gcccacactg ttcaggaaga attgcgcaac gttgatgcgg acattccgct ttcgaacgtt
     1681 cgcacgctcg acgccgtcgt ggccgattca attgcgccac gccgcctttc ggttgtcttg
     1741 ctcagtgcat tttccgggat cgcgttactg ctcgcgtcgg tgggcatgta tggtgtgatg
     1801 tcattcctcg ttgttcagcg cacccacgaa atcggcgtcc gcatggcgct tggagcgcaa
     1861 cgttccgatg tgttgagaat ggtaatcggc catgccgcga aactgttggg tatcggcgct
     1921 gccataggtt tgccaatcgc gttcttcagc aactcggcgc tccggacatt gctctatcaa
     1981 gtgggtccgt tcgacctctc aatcttcctg atcgtgacgt tgatcctcgc cggtgtcgct
     2041 ttgctcgcca gttacatccc ggccgtgcgc gccacacgcg ccgatccgat gctcgcactg
     2101 gcgcacaatg cctaaggagt catgagcacg ttgcttcacg acattcgtta cggcgtgcga
     2161 atgctgctta agcacaaagg cttcacggcc gtggccgtga tcgcgctcgg actcggcatc
     2221 ggcgccaata ccgccatctt cagcttggtg aacggtgttc tcctccgtcc gcttccgttt
     2281 ccggacgccg aacgcatcat ctacatcgaa ggaaaaaatc ctgcggcagc gggcattacc
     2341 gaaagcaaca tctcgtttct cgactttacc gattggtcgc aacaaaccga tctcttcgcc
     2401 agcaccgccg cctactggac cgggaccgcc aactttggtg cggatggggc ggagccggag
     2461 cgcgtgccgc gcgccggtgt gacgaccggg ttcttctccg tgctcggtg
//
LOCUS       DASTDG010000249         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_896589, whole genome shotgun sequence.
ACCESSION   DASTDG010000249 DASTDG010000000
VERSION     DASTDG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896589"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFO30_08165"
     CDS             complement(<1..384)
                     /locus_tag="VFO30_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419301.1"
                     /translation="MRTAIRTATVAIALLSWLAISNHCAFAGVALKSQGAKSECPFHS
                     KPARPQNPLSGIQCCKILRAITTAPVKAVARVMLDVADVDLPVDATAVSRAAHVTFVP
                     ETVGTGPPGTTSFVELIGSVQAHAPP"
     gene            complement(417..1706)
                     /locus_tag="VFO30_08170"
     CDS             complement(417..1706)
                     /locus_tag="VFO30_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HET9419302.1"
                     /translation="MRSRKAAVAFILVTVTLDILAMGLIIPVLPKLILDFLHGKMTSA
                     AEWNGWFGLVFALMQFFFSPVLGVLSDQFGRRPIILLSNLGLGLDYVVMALAPSLGWL
                     FLGRIISGITTSSIPTAMAYIADVTPKEKRAGAFGMIGVAFGVGFALGPALGGLLSNF
                     NPRLAFWVAATFSLANWVWGYFFVPESLGTNQRKPFELRRANPLGSLVLLRSHSELWR
                     LATIQFLAYTAHNVFSVWALYAIYRYAWNQLSIGISLMIVGICTAVISGGLTGRMVKR
                     FGERRTLYTGQFFGATGMFIAGLARHGSLYMGSVPIISLWNMSMPAAQSLMTRRVSER
                     EQGALQGAIGSLRSITFIIGPVLFSQIFSFFIDPKRSFHVPGAPFYVGGALLFTAMLM
                     ARRVEQPTFGVRSTAPMSLADAVPPESVSAIAPPEKI"
     gene            1803..2315
                     /locus_tag="VFO30_08175"
     CDS             1803..2315
                     /locus_tag="VFO30_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET9419303.1"
                     /translation="MPPLSYRSPKAEIRESKIHGRGLFASEEIAKDEIVAVKGGHIVT
                     REQMEEIRARLGPVEIQIDDALFIAPVTFEERESSMLYTNHSCDPNIGMRGEITFVAM
                     RDIRADEELMHDWATTDDDDYSVQCNCRSSTCREIITGKDWQKSELQTRYRGYFSAYL
                     AKKIAAARQS"
BASE COUNT          587 a          681 c          736 g          448 t
ORIGIN      
        1 aggcggagcg tgtgcttgaa cgctcccgat caattcaacg aatgaagtcg tgcccggtgg
       61 accggtgccg actgtctcgg gcacgaaagt gacatgcgca gctctcgaga ccgccgtcgc
      121 atcgacggga agatcgacat ctgcgacatc gagcatgacg cgcgcgacgg ctttcaccgg
      181 cgcggtggta atcgcgcgca agatcttgca gcattgaatt cccgagagag ggttttgcgg
      241 ccttgcgggt ttcgaatgaa acgggcattc gctcttcgca ccctgcgatt taagcgcgac
      301 accggcaaaa gcgcagtgat ttgagatcgc cagccaggac aaaagcgcaa tcgccaccgt
      361 agcagtgcgg atcgccgttc tcacctgcgc ttcaatcgcc gatccaggtc gtgcgctcaa
      421 attttttctg gtggagcaat ggccgaaacg ctttccggcg gcaccgcgtc ggcgagcgac
      481 atgggagctg tcgatcttac gccgaacgtc ggctgctcga cccgcctcgc catgagcatg
      541 gcagtgaaga gcagcgcccc gccgacatag aacggcgcgc ccggcacatg aaacgaacgt
      601 ttcggatcga tgaaaaagct gaagatttgg gagaataaaa cggggccgat aataaaagta
      661 attgagcgca agctgccaat tgcgccctgc aatgcgcctt gttcgcgttc gctcacgcgc
      721 cgcgtcatca gtgactgcgc cgctggcatc gacatgttcc agagagaaat aatcggcacc
      781 gaacccatgt agagcgagcc gtggcgggcg aggccagcga tgaacattcc ggtggccccg
      841 aagaattgac ccgtgtaaag ggtgcgccgt tcgccgaaac gcttgaccat ccgaccagtc
      901 aagccgccgg agattacggc cgtgcaaata ccgacgatca tgagcgagat gccgatgctc
      961 agttggttcc aagcgtagcg ataaatcgcg tagagcgccc agacgctaaa gacgttgtgc
     1021 gcggtgtagg cgagaaactg aatcgtcgcc aggcgccaca gttccgaatg ggagcggagc
     1081 aacacgagcg agccgagtgg attggcgcgg cgcaactcga acggcttccg ttggtttgtg
     1141 ccgagcgatt cgggaacgaa gaaataaccc cacacccagt tggcgaggct gaacgtggcc
     1201 gccacccaga aggcgaggcg cggattgaaa ttgctgagca acccgccgag cgccggaccg
     1261 agcgcgaagc cgacgccaaa cgcaacccca atcattccga acgcgccggc acgtttctct
     1321 ttcggcgtta catcggcgat gtaggccatc gcagtcggaa tactcgaggt ggtgatgcca
     1381 gagatgatgc gaccgagaaa gagccagccg agcgacggcg cgagcgccat gacgacgtaa
     1441 tcgagtccaa ggccgagatt cgacagcaga atgatcggcc ggcgtccgaa ctggtcggac
     1501 aaaacgccaa gcaccggcga aaagaaaaat tgcatcagag cgaagacgag cccgaaccag
     1561 ccgttccatt cggctgcgct ggtcatttta ccgtggagaa aatcgaggat cagttttggc
     1621 aggaccggga tgatcaaacc catcgccaga atgtcgagcg tgacggtgac gagaatgaac
     1681 gcaacagccg ctttgcggga tctcattgcc taggtgatct gtgacgagtg atgagtgacg
     1741 agtaagcaga gcagcgatct tttcgcttgt cacccgtcac ttgtcactcg tcactggttc
     1801 ttgtgcctcc actctcatat cgctcgccga aggccgaaat ccgtgagagc aaaatccacg
     1861 gacgcggttt gtttgcgagc gaggagatag cgaaagatga aattgtcgcc gtcaaaggcg
     1921 gtcacatcgt cacgcgcgag cagatggagg aaattcgcgc gcgccttggg ccggttgaaa
     1981 tccagatcga cgacgctctg ttcatcgcgc cggtcacgtt tgaagagcgc gaatcgtcga
     2041 tgttatacac caatcactcg tgcgatccaa acatcggtat gcgcggcgaa atcacttttg
     2101 tcgcgatgcg tgatatccgc gccgacgaag agttaatgca cgattgggcc acgacggacg
     2161 acgacgatta ttccgttcaa tgcaattgtc gatcttccac atgccgcgag atcataaccg
     2221 gtaaagattg gcagaagtcc gagctgcaaa cgcgttaccg cggctatttc tcggcgtatc
     2281 tcgcaaagaa aattgcagcg gcgcgacaga gctaaaggcg atcaccaagg cgcgcgcctt
     2341 gtggttcggt cgaaaccgga acaatgcgcc agaagccgtg ttcgtcttgg ataacgtgtc
     2401 gctcaccaga atcgccgagt ggaaactcga tgattcgccg gcttttgagc gc
//
LOCUS       DASTDG010000250         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1517328, whole genome shotgun sequence.
ACCESSION   DASTDG010000250 DASTDG010000000
VERSION     DASTDG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517328"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..157)
                     /locus_tag="VFO30_08180"
     tRNA            complement(73..157)
                     /locus_tag="VFO30_08180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Ser,seq:tga)
     gene            complement(212..1006)
                     /locus_tag="VFO30_08185"
     CDS             complement(212..1006)
                     /locus_tag="VFO30_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9419304.1"
                     /translation="MILKSRHNPIERLDFIIAGAQKAGTSALADFLETHPRIQMPHKD
                     ELHRTVQPARHFFDDEERFAKREVDYSPLQRGCVRKRPSNLLGSCTPIYIYWKTALER
                     IRNYSERVKLIILLRDPSSRAFSHWNMQRDRNLESLDFLDAVRAEKQRAEEVRPFQLR
                     KYSYVDRGFYGDQMERVFRVFRREQVQVIKFEDFRRNPRRTLDETCRFLGIELLCKID
                     NRENNSTPYARKMTRSERQTLVDLYRADIERLEELLGWDCSDWKKV"
     gene            1108..2172
                     /locus_tag="VFO30_08190"
     CDS             1108..2172
                     /locus_tag="VFO30_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET9419305.1"
                     /translation="MQGPIHGRGASWSPPNRFEKLHLDLADADFADVDRNDDEKPSPQ
                     TQFIRDDTKAIIARNQSPDVGFETSINPYRGCEHGCIYCFARPTHEYLGFSAGLDFES
                     KIVVKHDAPRLLEAELSSPKWKPQILMMSGVTDCYQPVERKLRLTRSCLEVLAKFRNP
                     VGILTKNRLVTRDIDVLSELARHNAVAVNLSVTTLDIDLQRIMEPRTSAPAARLEAVA
                     QLRAAKIPVGVMVAPVIPGINDHEIPAIVAACAKAGAQFAGYVLLRLPWAVAPLFDRW
                     LDEHFPERKTKVLGRVRHTRGGERLYDSRWRKRQIGEGVFAEQIATMFEVSCRRAGLA
                     EAPKLSAASFRRPNEQLTLL"
     gene            complement(2262..3626)
                     /locus_tag="VFO30_08195"
     CDS             complement(2262..3626)
                     /locus_tag="VFO30_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9419306.1"
                     /translation="MAIASINPATGEKLKEFAPHDSVEIEKRLKCAERAFAQHRREPF
                     ARRSELMMATASLLEREKEDFARLITLEMGKLLRDAVTEIEKCARCCRFYAENAEHLL
                     EDEPAQTDAARSYVRYEPLGPVLAIMPWNFPFWQVFRFAAPALMAGNVALLKHAANVP
                     QCALAIEQIFRRAGFDDGVFQTLLIESDHVAKLIGDERIKAVTLTGSEQAGSAVGSAA
                     AREIKKTVLELGGSDAFIVMPSADFQSAVETALKARTINSGQSCIAAKRFFIADTVYD
                     NFLRQFVDRMRALKVGDPMDETTEIGPLATEQILNGVHDQVQKSIAAGAKLLAGGNRI
                     AGPGFFYEPTVLVDVPREAPAFREEVFGPVAAVLRVREAADAIEFANDSRFGLGASVW
                     TNDRAEQELFASELESGMVFVNAMVASDPRLPFGGVKRSGFGRELGAHGIREFTNIKT
                     IWIS"
     gene            complement(3635..5125)
                     /locus_tag="VFO30_08200"
     CDS             complement(3635..5125)
                     /locus_tag="VFO30_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9419307.1"
                     /translation="MTRIRKADFLITFCCVPAFLICFAASAAEPVAIGSKKFTESYVL
                     GEIAERTLKDAGVAAEHRQGMGGTIILWQALRSGQIDVYPEYTGTIAEEILKDRELSS
                     RQQLRQALAKSGIGMTEPLGFNNTYALVMRRSEAQRLAINVISDLRVHPELKVGLTHE
                     FLDRKDGWRPLAAQYRLNPRRVLGIDHALGYAALRSSEIDVKDAYSTDAKIAEFDLVT
                     LTDDQNFFPRYDAVFLFRTSLPDVATAALRKLQGTLDEAKMIRLNAEAERTKNYARAA
                     SLYFDDVRSTSTIGESFPHRLARWTLRHLELAGISLLLSIAVGIPLGIWASRGGVAGQ
                     TILGFAGVVQTIPSLALLALLVPLPFFGISARTAIAALFLYGLLPIVRNTATGLQDIA
                     PAIRDSAVALGLGPSARLRKIYLPIASRSILAGIKTSAVINIGTATLAALIGAGGLGE
                     PILSGLNLNDNATILQGAIPAAVLALIVQSIFDLLDRVLIPKGLRL"
     gene            complement(5122..>5609)
                     /locus_tag="VFO30_08205"
     CDS             complement(5122..>5609)
                     /locus_tag="VFO30_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9419308.1"
                     /translation="GGLFPHLTARANVLLMPRHLAKPEEESRARLRELCALTRFPENL
                     LQRYPLELSGGQRQRVSLMRALVLSPELLLLDEPLGALDPLVRASLQKDLKQIFAGLH
                     QTALLVTHDLAEAAYLGDEVVLMDEGRIVQQGSIVDLRERPANEFVTEFINAQRSLAV
                     L"
BASE COUNT         1156 a         1623 c         1639 g         1191 t
ORIGIN      
        1 gactgctaag tggatttgtc gtctggagaa tcgccgtcca aaagagtggc ggttgagacg
       61 agagttaatc ggcggagggg gtgagattcg aactcacgag gcctttcggc cttccggttt
      121 tcaagaccgg cgcaatcaac cactctgcca cccctccgat ctagagccgg catcgttgac
      181 gccggatcgc ggtcaccgac cgcggctaca atcacacttt tttccaatcg gaacagtccc
      241 agccgagaag ttcttccagc cgctcgatgt cagcacggta aagatcgaca agagtttgcc
      301 gttccgaccg cgtcattttc cgggcgtatg gcgtggaatt gttttcacga ttgtcgatct
      361 tgcacagcag ttcgattccg agaaacctgc aggtttcgtc gagtgtacgg cgtgggtttc
      421 gccggaaatc ctcgaacttg atcacctgaa cctgttctcg ccggaaaaca cgaaagacgc
      481 gctccatctg gtcgccgtaa aatccgcggt cgacgtagga gtattttcga agctgaaacg
      541 gccgcacttc ctcggcgcgt tgcttctcgg cgcgaactgc gtcgagaaaa tcgaggctct
      601 ccaggttccg gtcccgctgc atattccagt gcgaaaaggc gcggctgctc ggatcccgca
      661 acaggatgat caacttgact cgctcgctgt agtttcgaat ccgctccagc gcggttttcc
      721 agtagatgta gatcggcgtg cacgagccga gcaggttaga cgggcgtttg cgcacacaac
      781 cgcgttggag tggcgaatag tcgacctccc gcttggcaaa gcgctcctcg tcatcaaaga
      841 aatggcgggc cggctggact gttcggtgca gctcgtcctt gtgcggcatc tggatgcgcg
      901 gatgtgtttc gaggaaatcg gcgagcgcac tcgtgccggc cttttgtgcg ccggcaatga
      961 tgaaatctaa cctctcaata gggttgtgcc tggatttcaa aatcacggcg atcagggatg
     1021 cggggaaacg ggtgtgcagc ttcgattaaa gcactttggt gtcaaggtag tgctcgacag
     1081 cgcctcatgt tcatgcgaac atagaccatg caggggccga ttcatggccg cggcgcctcg
     1141 tggtctccac cgaatcgatt cgagaagctt cacctcgact tggccgatgc cgactttgcg
     1201 gatgtggatc gtaacgatga cgaaaagccc tctccccaga cgcaattcat tcgggacgat
     1261 acaaaggcaa ttatcgcgcg taaccaaagt ccagacgtcg gcttcgagac cagtatcaac
     1321 ccatatcgcg gttgtgagca cggctgtatc tactgttttg cccggccaac acatgagtat
     1381 ctcgggtttt ccgccggcct cgatttcgag agcaagattg tcgtaaaaca cgacgcccct
     1441 cggttgctcg aagccgaatt gtcgtcgccg aaatggaagc cgcagatcct gatgatgagc
     1501 ggggtgaccg attgctatca gccggttgaa cgaaagctgc ggctaacccg gagctgtctc
     1561 gaagtgctcg caaaatttcg aaatcctgtc ggcattctga ccaagaacag attggtgacg
     1621 cgcgatatcg atgttttatc agagctcgcg cgacataatg ccgtcgcggt gaatctttct
     1681 gtgacgacgc tcgatatcga tcttcagcgc attatggagc cgcgtacgtc cgctccggcg
     1741 gcgcgcctcg aggccgttgc gcaattgcgt gcagcaaaga ttccagtcgg cgtgatggtc
     1801 gcgccggtga ttccggggat taacgatcac gagattcccg caattgtcgc agcgtgcgcc
     1861 aaagcaggcg cgcaatttgc cggttacgtg ttgttgcgtt tgccgtgggc ggtcgcgccg
     1921 ttgttcgacc gttggctcga tgagcatttt ccggaacgca aaacgaaggt gctcgggcgc
     1981 gtgcgacaca cccggggcgg agagcggctc tacgattcgc gctggcgcaa acgtcagatt
     2041 ggtgagggcg ttttcgccga gcaaatcgcg acaatgttcg aagtgagttg ccgtcgtgcc
     2101 ggtttggcag aggcgccaaa actttcagcc gcttccttcc gccggcctaa cgaacagcta
     2161 acattgttgt agaagcgcgt gtgccaagct cgcccgttcg agatttccgc ggttgagaca
     2221 aacgtcacta caatttcttc ggttgcaccc tccgcttgcg cttacgagat ccagattgtt
     2281 ttgatgttcg tgaattcacg gatgccgtgc gcgcccagtt cgcgaccgaa cccggagcgc
     2341 ttcacgccgc caaacggcaa gcgcgggtcg gaggcaacca ttgcgttgac gaagaccatt
     2401 ccgctctcga gctcggacgc gaaaagttcc tgctcggcgc ggtcattcgt ccaaacactc
     2461 gcgccgaggc cgaaccggct gtcattggca aactcgatcg cgtcggccgc ttcgcgcacg
     2521 cggagaacag ccgcgacggg tccgaaaact tcttcgcgga acgccggcgc ctcgcgcggc
     2581 acatccacaa gcactgtcgg ttcgtagaaa aatccggggc cggcaattcg attgccacca
     2641 gccaacaatt ttgcgcccgc cgcaatcgac ttctgcactt ggtcgtgaac gccgttcagg
     2701 atctgctcgg tggcgagcgg tccgatttcg gtcgtttcat ccattgggtc gccaacttta
     2761 agtgcgcgca tgcggtcgac gaactgccgc aaaaagttgt cgtaaacggt gtccgcaatg
     2821 aagaagcgct tcgccgcgat gcacgattga ccgctgttaa tcgtgcgcgc ctttaatgcc
     2881 gtttcgactg cgctttgaaa atctgcactc ggcatcacga tgaatgcgtc gctcccgcca
     2941 agctcgagca cagttttctt gatctcgcgt gcggcggcgc tgccgactgc gctcccggct
     3001 tgttcgcttc ccgtgagcgt gactgcctta attctttcat cgccaatcaa cttggcgacg
     3061 tgatcggatt cgatgagcaa agtttggaac acgccgtcat cgaatccggc gcggcggaaa
     3121 atctgctcaa tcgccagcgc gcactgcggc acattggctg catgcttgag aagcgcgacg
     3181 ttccccgcca tcagcgcggg cgcggcgaat cgaaacacct gccagaacgg aaaattccat
     3241 ggcatgatcg cgagcacggg cccgagcggc tcataacgaa cgtaactgcg cgcggcgtcg
     3301 gtttgcgccg gttcgtcctc taaaaggtgt tcggcattct cagcgtaaaa gcggcagcag
     3361 cgggcgcact tttcgatttc cgtaacggca tcgcgcagga gtttgcccat ctccaacgtg
     3421 atcaaccgcg cgaaatcctc tttctctcgt tcgagcaaag acgctgtcgc catcatcagc
     3481 tccgaacgtc tggcgaacgg ctcccgccga tgttgggcga acgcgcgctc ggcgcatttc
     3541 agccgtttct cgatttcaac tgaatcgtgc ggcgcaaatt ccttcagctt ctcgccggtc
     3601 gccggattga tcgacgcaat cgccattgtc gatcttacag ccgaagtccc ttcggaatca
     3661 aaacgcgatc gagtaaatcg aagatcgact gcacgatcaa ggccagcact gctgcgggaa
     3721 ttgcgccctg caaaatcgtc gcgttatcgt tcagattgag gccgctcagg attggttcgc
     3781 cgagtccgcc tgcgccaatc aatgcggcga gtgtggcggt gccgatgttg attactgcgc
     3841 tggttttgat gccggccaaa atcgagcgcg acgcgatcgg caaatagatt ttccgcagcc
     3901 gcgcgctggg cccgaggccg agcgcgacgg ccgaatcgcg aatcgccggc gcgatatcct
     3961 gcaagccggt cgcggtgttg cgcacgatcg gcagcaagcc gtaaagaaag agcgccgcga
     4021 tggcggtgcg cgcgctgatc ccgaagaatg gcagcggcac cagcaaggcg agcaaagcga
     4081 gcgacggaat cgtttgcacg actcctgcga agccgagaat tgtctggcca gccactccgc
     4141 cgcggctagc ccaaatgcct aacggaatcc cgacggctat cgagagcagt aatgaaatgc
     4201 cggcgagctc gaggtgccgc agcgtccagc gcgcgagtct gtggggaaat gattcgccga
     4261 ttgtggatgt cgatcttaca tcgtcgaaat acaagctcgc cgcgcgcgcg tagttctttg
     4321 ttcgctctgc ctcggcgttg agtcggatca tctttgcttc gtccaaggtt ccttgaagct
     4381 tgcgcagcgc agcggtcgcg acatccggca gcgatgtgcg aaacaaaaac actgcgtcat
     4441 agcgcgggaa gaagttctga tcgtcagtca gcgtgacgag atcgaactcc gcgatcttcg
     4501 catcagttga gtaggcgtct ttcacgtcta tctcgctgct gcgtaaggcc gcgtaaccga
     4561 gcgcgtgatc aattccgagg acgcggcgcg gattcagccg atattgcgca gcgagcggcc
     4621 gccaaccgtc ttttcggtcc aaaaattcat gcgtgagtcc aaccttcagc tcggggtgga
     4681 cgcgtaaatc gctgatcacg tttatggcga gccgttgcgc ttcgctgcgt cgcatcacga
     4741 gcgcgtaggt gttgttgaac ccgagcggct cggtcattcc aattccggac ttcgccagcg
     4801 cctggcgcaa ttgttgccgc gaactcaatt ctcggtcttt caaaatctct tcggcgattg
     4861 tgccggtgta ttcgggatag acatcgatct ggccgctgcg caatgcctgc cagagaatga
     4921 tcgtgccccc cattccctgg cgatgctcgg cggcaacgcc ggcatccttc agcgtgcgtt
     4981 cggcaatttc accgagcaca tatgattcgg tgaatttctt cgagccgatg gccaccggct
     5041 ccgctgcgga agcggcaaaa caaatcagga aagctggaac gcagcaaaac gtgataagaa
     5101 aatctgcttt cctgattctc gtcataaaac agcaagactg cgttgcgcat tgatgaattc
     5161 ggtcacgaac tcattcgccg ggcgctcacg aagatcgaca atggaccctt gctgcacgat
     5221 tcgcccttca tccatgagca cgacttcatc gccgagatac gccgcttcgg cgagatcatg
     5281 cgtgacgagc agtgccgtct ggtgcaggcc cgcaaaaatc tgcttcaggt ccttttgcaa
     5341 agacgcgcgc acgagtgggt cgagtgcacc cagtggttcg tcgagcagga ggagctcggg
     5401 cgagagcacg agcgcgcgca tcaagctgac gcgctggcgc tggccgcccg agagctcgag
     5461 cgggtagcgc tgaagcaggt tttccggaaa gcgagtgagc gcgcaaagtt cgcgcaaacg
     5521 ggcgcgcgat tcctcttctg gtttagcgag atgtcgcggc atcaacagca cattcgcgcg
     5581 cgcagtcaga tgcggaaaca aaccgcctt
//
LOCUS       DASTDG010000251         7541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_618762, whole genome shotgun sequence.
ACCESSION   DASTDG010000251 DASTDG010000000
VERSION     DASTDG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618762"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(572..1081)
                     /locus_tag="VFO30_08210"
     CDS             complement(572..1081)
                     /locus_tag="VFO30_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419309.1"
                     /translation="MNAKRRWAMHCWTLTKGLPEPSEKNPQKFIRFIQLDCHEAENLY
                     LTDAVLTDLGYTWDSARNAITAAAANFGQKRAALERCIHWDRRRSDIKDVIDEIAVIL
                     DPKKVPWSLRVGNVIGRARPVGDLADFLGGSVITSLWAEKKQDESAVSAAQDANRHVC
                     VGISKSMNG"
     gene            complement(1419..3161)
                     /locus_tag="VFO30_08215"
     CDS             complement(1419..3161)
                     /locus_tag="VFO30_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9419310.1"
                     /translation="MVQMQDVMAKPMPEFREGSIVKGRILEIRPREVLVDIGYKSEGV
                     IPITEFEDVENLEVGDEVDVLLERLENDEGMVVLSKEKAAYRQNWNKIAGVYEEDGMI
                     KGKVKSVVKGGLMVNIGVEAFLPASQIDVVPPKDLQQFVGNTYDFKIVKINDDRRNVV
                     LSRRELIEQERSEKRQKFMDSVKVGDRVVGTVKNLTDFGAFIDLDGMDGLLHITDMTW
                     GRLGHPSELVKVGQQLEVVVLDINKEKERVSLGLKQTQKNPWDQIEERFPAGQRVKGR
                     ITNLVPYGAFVELEEGVEGLIHVSELSWTKRIMRPSDILSVGQEVEAVVLGVNKEEQK
                     ISLGLRQLEPNPWDEIEKKFTIGSRVKGRIRNMTAYGAFVELEEGIDGMIHVSDLSWT
                     RKINHPSEVFKKADEVEAEVIDIDKTNQRISLGVKQLTEDPWKNIDQKYKIGDLVKGK
                     VTKLASFGAFVQLADDIDGLVHISQLSEDHVAKVKDVLKVGQEVEARVIKVDKIERRI
                     GLSIKAANYSEEELRKEAETFDTLKPGEDMVGLEKAFAAAEQEEYRPGESRKPVKESP
                     PAKEPKRESKKPKK"
     gene            3433..3945
                     /locus_tag="VFO30_08220"
     CDS             3433..3945
                     /locus_tag="VFO30_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419311.1"
                     /translation="MLILRLSIFAALCAASSVCAEAPAEQQTVAAIKSPELTIVHLWA
                     PWCSNCQAELKSGGWLRTIKDNPQVKFYFVSVWNDGQDGRAMLQRFQIPDQPNVRILA
                     DPGPRRGENKIKQFSGLPLSWIPTTWIFKGGDLRYALNYGEVRFPVVQQFIEDSKSEW
                     SHKGESKLEE"
     gene            3959..5401
                     /locus_tag="VFO30_08225"
     CDS             3959..5401
                     /locus_tag="VFO30_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9419312.1"
                     /translation="MIELSTATTLAAKKPPAILSLIGDTPLVEITQFDTGPCQLFLKL
                     ENQNPTGSIKDRIALSMIEAAERDGQLQPGGTVIEATAGNTGLGLALVAGAKGYRVVL
                     VIPDKMSQEKISHVKALGAEVRLTRSDVTRGHPEYYQDMAARLAEELPGGFYVNQFGN
                     PANPLAHERTTGPEIWEQMRHDVDAIVCGVGSGGTLTGLGRFFNRVKPRRGIEMVLAD
                     PKGSVLYEYVKTGELVESGSWTVEGIGEDFVPEIADLSFVTDAFEIDDEESFATAREL
                     LRKEGIFGGSSTGTLVAGALRYCREQTQPKRVVSFVPDTGNKYLSKMYNDFWMAEQGF
                     IHRPPHGDLSDLILHRYEAGEVITVGPDDTLLTAFKRMRDTDVSQLPVVDQAGRAAGI
                     IDESDILVKVHRDATHFNDSVKNAMTDRLETLPPNAKIKDLLGVFDRGRVAIVMEGDK
                     FLGLITRTDLLSYLRLHMPKSSVPPSRECT"
     gene            5528..6688
                     /locus_tag="VFO30_08230"
     CDS             5528..6688
                     /locus_tag="VFO30_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET9419313.1"
                     /translation="MKKPHEFATRAIHAGQQPDPTTGAIMTPIYATSTYVQESPGKHK
                     GYDYSRSINPTRLAYEKCIADLESGTRGFAFASGLAAMATALDLLDSGSHVVVSDDLY
                     GGTFRLFEKVRRRSANLDFTYVDLTDASRFEGAIKKNTRMVWIETPSNPLLKIVDLEA
                     IAKIAREHRIISVCDNTFATPWIQRPVELGFDLVVHSATKYLNGHSDLVGGVMVVGDN
                     KALGDQVWFLQNSVGAIAGVFDSFLVLRSLKTLALRMERHCANALEIARWLEDQPQVR
                     SVSYPGLKSHPQHDLARQQMRGFGGMVTAVLKTDLGGTRRFLENTELFALAESLGGVE
                     SLINHPALMTHASVPKEQREALGVTDSLVRLSVGVEDVRDLIDDLQFAFEAI"
     gene            6774..>7541
                     /gene="aroC"
                     /locus_tag="VFO30_08235"
     CDS             6774..>7541
                     /gene="aroC"
                     /locus_tag="VFO30_08235"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9419314.1"
                     /translation="MGNSFGQLFRVTTFGESHGGGIGVVIDGCPPKIDISEADIQDEL
                     NRRRPGQSKITTQRKEDDVCEILSGVFDGKTLGTPIAILVRNKDARPEDYVDLAQTFR
                     PSHADFTYEAKYGIRNWQGGGRASARETIGRVAAGAIARKILRVLFPKIDIIAYVAQV
                     HDVAGKIDRSKVERRDVDSAIVRCPDRAAAERMTQLIEQTREFGDSLGGIIECVIREA
                     PPGLGEPVFDKLEADLAKAMLSLPATKGFEIGSGFAAT"
BASE COUNT         1583 a         2181 c         2076 g         1701 t
ORIGIN      
        1 aaaggatggc cagaaaagcc gacgctagga cctgcatcag gtaagttgcg tttgtcgtca
       61 ttgcgcaggc gtgtggcatc aaaatttatt caaagcgccg cgcgattgtc gatcttgtct
      121 tgcagcgcaa aaatttctgg cgctgcggaa atccttcgct aaaaaagccg gactttcgcc
      181 gcggccatgc atcttcgtgc gactgcggca ctcacgctga tttacttggt tcggtcgaat
      241 tcaagtcgcg aaccgctcga atcttgaaat gtcatgtggt cacgcgaaat cgtagtttga
      301 taaaccgacg tcccggatgc cgtctcgatc ttgattcgcc cgctgttccc aaacgtgtac
      361 ctaccgcgag tgctgccgat aataacctga ctgttattcg cgaattccca gacgaccgcg
      421 ttcggatcat tcgaggtacg ccatttaccg atgatatcgc gggaaggacc gccgcaagtg
      481 gtcaggacgc aggcaacaac tgccaacatg atggttatca gcggtcggga ttgatgtcgg
      541 ctgaaagtgc gcatcaccac ggacggactc gttaaccatt catgcttttg ctaatgccca
      601 cacaaacgtg ccgatttgca tcttgtgccg ctgaaactgc agattcgtcc tgcttcttct
      661 cggcccataa tgacgtgata acacttcctc cgagaaaatc cgccagatcg ccaactgggc
      721 gagctctgcc aatgacgttc cctactcgaa gcgaccaagg aactttcttc ggatcaagaa
      781 tgacggcgat ttcgtcgatg acgtctttga tatcagatcg cctacgatcc caatgaatgc
      841 acctttcaag cgctgctcgt ttttgcccga aattggctgc ggcggcagta atcgcgtttc
      901 tcgccgagtc ccatgtgtag cctaaatctg taagcaccgc atcggttaga tatagatttt
      961 cagcctcatg acagtcgagc tggataaatc tgatgaattt ctgcggattt ttctcgctag
     1021 gctcaggaag cccctttgtc agcgtccaac agtgcatagc ccatcggcgt ttcgcgttca
     1081 tcgcagagac tacgcagaat gacctcgagc ttgaattgat aggccttgat ttcttctccg
     1141 ttacacggca gtaccgcaag attgatcatg tgatggcggg ggactggctc caaatccggt
     1201 aatcgtcatc gccttccaca agaagcagag gtgcgccaaa caatggcccc atcaggacgt
     1261 gtccgccaag acatgccgcc acttctttcg tcacatcgtc aaatttttgg gctttatact
     1321 cagaccgtgt tctgtcgatg taaatcacgc tcgcgcgcgg gcctccactc tgctcggcgg
     1381 cctgccgaga gcgacagatg tggccgttgc gagcgcagtt acttcttcgg tttcttcgac
     1441 tcgcgctttg gctcttttgc tggcggtgat tctttgacgg gttttctcga ttcgccgggg
     1501 cgatattctt cctgttcagc cgcagcaaag gctttctcca agccgaccat atcttcgccg
     1561 ggcttgagcg tgtcgaatgt ctcggcttcc ttgcgcagtt cttcctcgga gtaattggcg
     1621 gctttgatcg acaacccgat gcggcgctcg attttgtcca ccttgatgac gcgggcttcg
     1681 acttcctggc cgaccttaag gacgtctttc actttcgcga cgtggtcttc gcttaactgc
     1741 gagatgtgaa caagaccgtc gatgtcatcg gcgagctgca cgaacgcgcc aaaactggcc
     1801 agcttggtca ctttgccctt caccagatcg ccgatcttgt atttctgatc gatgttcttc
     1861 catgggtctt cggtgagctg cttgacgccg aggctgattc gctggttggt tttgtcgatg
     1921 tcgatgactt cggcttcgac ttcgtcggcc tttttaaaga cctcgctcgg gtggttgatc
     1981 ttgcgcgtcc agctcaagtc cgagacgtga atcatcccat caattccttc ctccagttcc
     2041 acaaacgcgc cgtaggcggt catgttgcgg atcctgcctt tgacgcggct gccgatggtg
     2101 aacttcttct cgatctcgtc ccacggattt ggctcgagct ggcgcaggcc taacgaaatc
     2161 ttttgctctt ccttgttgac gccgagcacg accgcctcga cttcctggcc cacgctcaga
     2221 atatcggaag gccgcataat tcgcttcgtc catgacaact ccgagacgtg gatcagtcct
     2281 tcaacacctt cctccaactc aacgaacgca ccatacggga cgaggttggt aatcctgcct
     2341 ttaacgcgct gacccgcagg gaaacgctcc tcgatctggt cccagggatt cttctgcgtc
     2401 tgcttcaggc ctaacgagac ccgttctttc tctttgttga tgtcgagaac gacaacctcg
     2461 agctgctgcc ccactttgac cagctcgctc ggatgaccga gccggcccca cgtcatgtcg
     2521 gtgatgtgga gcaagccgtc catgccgtcg aggtcgatga acgcgccgaa gtcggtcaga
     2581 ttcttcaccg ttccaacgac gcggtcgccc accttgacgc tgtccatgaa tttctggcgc
     2641 ttttcgctac gctcctgctc gattaattcg cgccggctca ggacgacgtt gcgcctgtca
     2701 tcattgatct taacgatctt aaaatcgtaa gtattgccga cgaactgctg caaatccttc
     2761 ggcggcacga catcgatctg cgaggcaggc agaaatgctt ccacgccgat gttcaccatc
     2821 agcccgcctt tcacgaccga cttcactttg cctttgatca tcccgtcttc ctcgtaaacg
     2881 ccggcaattt tgttccaatt ctgccgatac gcggccttct ccttggaaag cacgaccatg
     2941 ccttcatcgt tctcgagccg ctccagcaac acgtcgactt cgtcgcccac ttcaaggttt
     3001 tccacgtctt caaattccgt gattgggatg acaccttcgg acttgtaacc gatgtcgacc
     3061 aacacttcgc gcggccgaat ttccaagatg cggcctttga cgatggagcc ttcgcggaac
     3121 tccggcatcg gcttcgccat cacatcctgc atttgcacca taagtaaaaa gactcctcag
     3181 cctggtggtc ccaaaacctt tttgggacca ggattcccgc gcagccacag cagcggaaaa
     3241 cggagcgcca cagtagcgaa gttacgctgc gcggtcaaac aaacggtttg ggaacacggg
     3301 catcttgctc gatgtgcggc gcacctgcgg cgaacgcttt cgttcggccg gagccgaaca
     3361 aggccgatgg aagcctggtg ctccccggtc gcgtgttgaa cacacggcat ttcgccttac
     3421 gctgagacga gaatgttgat attgcggtta tcgatcttcg ccgcactctg tgcggcgagc
     3481 tccgtttgcg ctgaagcgcc ggccgaacaa caaaccgttg ctgcgatcaa atcgcccgag
     3541 ctgacgatcg tccatctttg ggcgccgtgg tgctcgaatt gtcaggctga gttgaaaagc
     3601 ggcggctggc tcagaaccat caaagacaat ccgcaggtga aattttattt cgtgtcggtg
     3661 tggaacgacg gtcaggatgg ccgcgcaatg ttgcaacgct tccaaattcc cgaccaaccg
     3721 aacgtccgta tcttggctga tccagggccg cgccgcggcg agaacaagat caaacagttc
     3781 agcggcctgc cgctctcatg gattccgacg acgtggattt tcaaaggcgg cgatctacgt
     3841 tacgcgctga actacggcga ggtgcgcttc ccggtcgtgc aacagttcat tgaggacagc
     3901 aaatcggagt ggtcgcacaa gggcgaatca aagctcgagg aatgaggaca agatcgacat
     3961 gattgagctt tcgacggcga cgacgctggc tgcgaaaaaa ccaccggcca ttctttcact
     4021 catcggcgac acgccgctcg tcgagatcac acaattcgac accgggccgt gtcaactttt
     4081 cctgaaattg gaaaatcaga atccgaccgg ttcgatcaag gaccgcatcg cgttgtcgat
     4141 gattgaagcg gccgagcggg atgggcagtt gcagccaggc ggcacggtaa ttgaagcgac
     4201 tgccggcaac acgggcctcg ggctggcgct cgtggccggc gcgaaaggtt atcgcgtcgt
     4261 gctcgtcatt cccgacaaga tgtctcagga aaaaatctct cacgttaagg cgctcggcgc
     4321 ggaggtgcgt ctgacgcggt cagatgtgac gcgcggccat cccgaatact accaggacat
     4381 ggccgcgcga ttggccgagg aactgccggg aggattttac gtcaaccagt ttggtaatcc
     4441 ggcaaatccg ctcgcgcacg agcgcacaac tggcccggag atttgggaac aaatgcgaca
     4501 tgacgtcgac gcgatcgtct gtggcgtcgg atccggcggc acgctgaccg gacttggccg
     4561 cttcttcaat cgcgtcaagc cgcgacgcgg cattgaaatg gtcttagctg acccgaaagg
     4621 ctcggtgctt tacgaatacg tcaagaccgg cgagctggtg gagtccggca gttggacagt
     4681 cgaggggatc ggcgaagatt ttgttcctga aatcgctgac ctgtcgttcg tgacggacgc
     4741 gtttgagatt gacgacgaag aaagcttcgc gactgcacgc gagcttctgc gcaaggaagg
     4801 aatcttcggc ggctcatcca cgggcacact cgtggcgggc gcgctgcgtt actgtcgcga
     4861 acaaacgcag ccgaagcggg tcgtcagctt cgtcccggac acgggcaata aatatctgtc
     4921 gaagatgtat aacgactttt ggatggccga gcaggggttt attcatcggc caccgcacgg
     4981 tgacttgagc gatctgattt tgcaccgcta cgaagccggc gaggtcatca ccgtcgggcc
     5041 cgacgatacg ctgctaaccg cattcaaacg catgcgcgac accgatgtct cacagttgcc
     5101 ggtcgttgac caagctggcc gcgccgccgg cattatcgat gaatcggata tcctggtgaa
     5161 agtccatcgc gatgcgacgc atttcaatga ctcggtgaaa aatgcgatga ccgaccggct
     5221 cgagacgctg ccaccaaacg ccaagattaa agatctgctc ggagtgttcg accgtggccg
     5281 cgtcgccatc gtgatggagg gcgacaaatt tctcggcctg atcacgcgca ccgatttgct
     5341 ttcctatcta cgcctgcaca tgccgaaatc gtccgtgccg cccagccgcg agtgcacgtg
     5401 agaattaatc tcggagccat gaaaccggga aatgaattag aagcagaatt tccggcgtct
     5461 ctgctttgct gatttagctt tcccgggttc ctggtttcca gatcatctgc atctaagtta
     5521 gatcgacatg aaaaaaccgc acgagttcgc cacgcgcgct attcacgccg ggcagcaacc
     5581 ggacccgacc acgggcgcga tcatgacgcc gatctacgcc acatctactt acgtgcagga
     5641 gagcccgggc aaacacaaag gctacgacta ctcgcgctcg atcaacccga cgcgcctcgc
     5701 ctacgaaaaa tgcattgctg acctggaaag cggcacgcgc gggtttgctt tcgcctccgg
     5761 tctcgctgcg atggcgactg cgctcgatct tctcgacagc ggttcgcacg tcgtggtcag
     5821 cgacgacctt tacggcggca cgttccgcct ttttgaaaaa gtccggcgcc gctccgcgaa
     5881 cctcgatttc acatacgtcg acctcacgga cgcgtctcgt tttgaaggcg caatcaaaaa
     5941 gaatacgcgg atggtctgga tcgagacacc gagcaatcca ctgctcaaaa tcgtcgattt
     6001 ggaggcgatc gcgaagatcg cgcgcgaaca ccgcatcatt tcggtctgcg acaacacttt
     6061 tgccacgccg tggatccagc gtccggttga actcggtttc gatctggtgg tgcactccgc
     6121 caccaaatat ctcaatggcc actccgattt agtcggcggc gtcatggttg taggcgacaa
     6181 caaagcactc ggcgatcaag tctggtttct ccagaactcg gtcggcgcga tcgcgggtgt
     6241 gttcgacagc ttccttgtct tacgaagttt gaagacgctg gcgttgcgaa tggagcgcca
     6301 ttgcgcgaac gcgctcgaga tcgcgcgctg gctcgaagat cagccgcagg tcaggtctgt
     6361 cagttacccc gggttgaaat cgcatccgca gcacgacctc gcgcgccaac agatgcgcgg
     6421 ctttggcgga atggtgacag cggttctaaa aaccgacctc ggcggcacac gccgtttttt
     6481 ggaaaacacg gagctgtttg ccctggccga aagccttggc ggcgtcgaaa gcctgatcaa
     6541 tcatcccgcg ctcatgacgc acgcctctgt gccaaaggag caacgcgagg cgcttggcgt
     6601 cactgactcc ctcgttaggc tttcggtcgg cgtggaagac gtccgcgatc tgatcgacga
     6661 tctgcaattc gccttcgaag cgatctgagc gctggggagc acacgcgagt cgcgcgcgct
     6721 ccccggacat tgcgcgttgt catcgtgcga catcgactta acttgctcgc ccgatgggca
     6781 acagctttgg acaacttttt cgcgtcacca cctttggcga gtcgcacggc ggcgggatcg
     6841 gtgtggtgat cgatggttgc ccgcccaaga tcgacatcag cgaagcggat attcaagacg
     6901 aacttaaccg ccgtcgaccg gggcagagca aaatcacgac gcaacggaaa gaggacgacg
     6961 tgtgcgaaat cttgtctggt gttttcgacg gcaagaccct cggcacgccg attgccattc
     7021 tggtccgcaa caaagacgcg cgtccggagg attatgtcga tcttgcgcaa acgtttcgcc
     7081 catcgcacgc cgatttcact tacgaggcga agtatggaat tcgaaactgg caaggtggcg
     7141 ggcgtgcctc cgcgcgtgag acgatcgggc gcgttgcggc cggtgccatc gcgcgaaaaa
     7201 tcttgcgcgt gctctttcca aagatcgaca tcatcgccta tgtcgcgcag gtgcacgacg
     7261 tcgccggcaa gatcgacagg tccaaagtgg aaaggcgaga tgttgattct gcgattgtac
     7321 gatgtcctga tcgcgcggcc gcggagcgca tgacccaatt aattgagcaa acgcgtgagt
     7381 tcggtgactc gttaggtggc atcatcgaat gtgtcattcg agaggctccg ccgggcttgg
     7441 gcgagccggt gtttgacaag ctcgaggccg atctcgcaaa agcaatgctg agtttaccgg
     7501 cgacaaaagg ttttgagatc ggttcgggct ttgctgcgac a
//
LOCUS       DASTDG010000252        18976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1076838, whole genome shotgun sequence.
ACCESSION   DASTDG010000252 DASTDG010000000
VERSION     DASTDG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 18976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18976
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076838"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFO30_08240"
     CDS             complement(<1..919)
                     /locus_tag="VFO30_08240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9419315.1"
                     /translation="MISRQVMPAASIVRIVDPLGDSEWDNLVSSHPDSTIFHTATWAK
                     VLVTTFGHRPVYLELTRASNLSALLPLMEISSHVTGRRGVCLPFIDCCNPLIFEGSDI
                     SPILHALSSLSRQRNWKYFELRCGGNSLHPVAPALVTLRGHNIDLRSGIEVVFSRFAS
                     SVRRAIRKAERSGLNVCLVSSHEAVLKFYRLHTLTRKRQGLPPLPLSYFLRVYDSVIE
                     PGLGFMVMVQKEARPVGAAVFFIYGKRAVYKFAASDLTFQNLRPNNLAMWEGIKSLMQ
                     GGAETLHLGATSMEGEGLRRFKLSWGSEEE"
     gene            1629..2552
                     /locus_tag="VFO30_08245"
     CDS             1629..2552
                     /locus_tag="VFO30_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9419316.1"
                     /translation="MRAVENIAASNKIDVILPVALDATAFIAKHATRLARLAALHPVP
                     DANTIETCRDKCLFAQFAAKHAIPTPQTVSFIGNARDDLGVFERGTPVLLKPRISSNG
                     RGIISFADKREAELFLDSHPELRSQFVAQPRFAGQDFGCNVLCLHGNIVAYTIQKAVF
                     RPRQPFGVAAGIEILHNDEVLEISRHLLRALRYHGVANIDLRYDQRERRIKVLELNAR
                     YWGTLLGSLFAGVNFPYLSCMAALGQPIDSPEYRHIQYFSAWGLGQQWLGALRGIDWP
                     PRSPAASVFRYFCADPLPDLMSPFLRRPPPP"
     gene            complement(2633..3106)
                     /locus_tag="VFO30_08250"
     CDS             complement(2633..3106)
                     /locus_tag="VFO30_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9419317.1"
                     /translation="MMRLISVALLGLLFVKLDGSILACDASDDTDFPSSMALAKQSNR
                     PLLLAFLGTDWSISSLKLDREVFDQAAFADNSKYNFLLCKIHFYQTQERDAKVLRQNR
                     DLAIKYHIEQFPTVVVLRPDGAELGRFGYISGGVEKFAAEVNKLISASHSSSSAQ"
     gene            3105..4406
                     /locus_tag="VFO30_08255"
     CDS             3105..4406
                     /locus_tag="VFO30_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9419318.1"
                     /translation="MSTKTIMRVRECQLALLKVWVTLVALNGAWLCDLARAEQIETVA
                     QVARRIDTRLQQMENMAADDTATALAQSFLTDLDLLKRQSIMVFGEREKRLQDASDNL
                     RRGIASWSTEAVGNPTRRRIVLADMRRTWAEMKSQWPTEALEILPPLWSCPMHSELIE
                     SAAGTCPVCGMPLEPIYVTQPQLSTDPIIRAAIFPKTPLQVGRKADLRLHLLFNKDGR
                     PVELSDLEEAHTRKIHLLIVDSSETDYHHEHPEPVGQGEYSFSFTPAREGIYRVWVDL
                     KPVLTHIQQYSVTDIATAGAPSADPPGNEPENRHTEIDGYKFDLAFDRPVVREKETAA
                     GTLHITGPDGGPCKRLEIVMGAFGHFVGFCEDFLTVLHVHPVQAATLTPESMGGPDLP
                     FYFRSIEPGTVRLFTQVKIDGKDFFPRFVLKVQPLRDAAAE"
     gene            complement(4506..5813)
                     /locus_tag="VFO30_08260"
     CDS             complement(4506..5813)
                     /locus_tag="VFO30_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HET9419319.1"
                     /translation="MRKILVSFFLLSSLLFIAFGLLRAVPAKAQVGSMAARTRPNVHS
                     AAAPNTARAGKKSDDEDEDCMCKDDPSDYPANVVPLASTATPTSDKPDLVPLLITSPT
                     TKPKQVAPIYVDAYQKPGKLLYRFDAVIVNQGGTLDLYQQGANATTYQVIWVGGNPST
                     QPDPNFFTSPDPSVATVEDRSSTGAYMRFVKLPGHNHFHINGAYSYSMAGRVISKAGV
                     GFCMLDGYRAFGSGWFKPGYIGVGPDTWCAHADPNAAFVHMGISPGKADYYPSQTADQ
                     WIDITGLAPGDYTLTGQVNPFGYMDESDTSNNTVQQTRTVPGAISNATAATCLNGVPS
                     TINFSGSVVGPDVPARLSASCKPSRTSTNCYIMQASTTQLTFAIASAPAHGTVSITSQ
                     NGLTATAQYTADIGYIGNDSFTYTATDTRNLTSLPATAQITVQ"
     gene            complement(6361..6579)
                     /locus_tag="VFO30_08265"
     CDS             complement(6361..6579)
                     /locus_tag="VFO30_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419320.1"
                     /translation="MSRTGNASVSGSPARRARNSKAKRKVRGKGKAAGSAHQDSEREM
                     AAVGSLTRTKALLLAALRVWELKHRIRD"
     gene            6709..7710
                     /gene="sppA"
                     /locus_tag="VFO30_08270"
     CDS             6709..7710
                     /gene="sppA"
                     /locus_tag="VFO30_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9419321.1"
                     /translation="MTDRKTGCLPAFLFVALCLSMFVNLLLVVASLHRFGRVVGTEEP
                     LPRFREVLVQRGAPATFDKVAVILLRGLISTAIPGNTTDSMVDDMRAALQQARDDDRV
                     KAVVLEIDSPGGEVTAADAIYNAVVKTRARKPVVVYMDSLAASGGYYIACGGKFLMAN
                     ETTITGSIGVIIQTLNYEQLFNKVGLASVVFKSGKFKDMLNGARPITPEERDLVQSFI
                     MKTYDKFLGIVASERKLPGDLLRNTIADGRILSGKDAYDNKLVDGLGQLDDAFAKAKE
                     LGKSPGAKIVKYGPPFSLSRFLRAFGQSSTIELQLPKQLVPQLESGRAYFLPSYYAP"
     gene            7735..8553
                     /locus_tag="VFO30_08275"
     CDS             7735..8553
                     /locus_tag="VFO30_08275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9419322.1"
                     /translation="MLWTSAGVTNVFAILFSIFLLLVGACIYLALILQVIARPSERAV
                     EATKTFGLPEAIFATSLTLFLLLGALGSLTSGTKQITDRVLAENLLFTLGIVVLITGL
                     LKFRGFNVNSLAGFTKTSFAHAALTGAALLLAAYPLIAFADAITQRLLGSGSSKQTIV
                     ELFNSSRTIDRRIMIIIFAVAIAPAAEEFLFRFFFYGVLRRYLGRFLGLVLNSLLFAA
                     VHAHLPSFAPLFVLGSCFTIAYEWSGTILVSMTMHALFNAVSLTVLAFPELFPQ"
     gene            8550..9440
                     /locus_tag="VFO30_08280"
     CDS             8550..9440
                     /locus_tag="VFO30_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9419323.1"
                     /translation="MKLSRVGEDRLLAALLASLRVAKSVVVSAGDDCAVVKIRASGDF
                     LVLKTDCVVEQVHFSSSAKPTDIGWKAMMRPLSDFAATSALPQFALVTLIVSRATEAE
                     WVKKIYRGLSRAAARFGVSIVGGETSSTPGPVTISIGVAGCVEKNRFVSRRGAKAGDD
                     LFVTGRLGGALRHRHLQFVPRIPESRWLTENFSIHAMIDLSDGLGTDLPRLARASKVG
                     FNIDMQNLPLSSGCNIDSALSDGEDYELLFAVSPRARKRLQERWRKKFPKLPLTRIGS
                     LNRQSAVGNRQLLGGYVHFQ"
     gene            9418..9831
                     /gene="tsaE"
                     /locus_tag="VFO30_08285"
     CDS             9418..9831
                     /gene="tsaE"
                     /locus_tag="VFO30_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9419324.1"
                     /translation="MATFISNSPAETEAFGREFARKIDKRDVVALQGELGAGKTQFVK
                     GFVAGIGSNAEATSPTFTLIHEYSGGRWPVYHIDFFRLEDRQSAARLGLDDYFFGDGV
                     SVIEWADKFPEFIPDSARWILFSTKSETQRAITLR"
     gene            9828..10433
                     /gene="tsaB"
                     /locus_tag="VFO30_08290"
     CDS             9828..10433
                     /gene="tsaB"
                     /locus_tag="VFO30_08290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9419325.1"
                     /translation="MKILALELSTARGSLAYLSDAAEFARDWPHERKNSAAFFANLAD
                     VQKQFGLPERMIVGLGPGSYAGTRIAISAAVGLQFASGAELVGLPSICAVECDAVEYC
                     VIGDARRQSFFFARICANELVEGPELLGEIELRAKMETLDVKMSIFASEELPQFPRVQ
                     VRYPSARVLARLARDANRGFVLPPLEPMYLREPHITVPKST"
     gene            10430..11518
                     /gene="alr"
                     /locus_tag="VFO30_08295"
     CDS             10430..11518
                     /gene="alr"
                     /locus_tag="VFO30_08295"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9419326.1"
                     /translation="MTDQYRCWAEIDRSALRHNAAIVRERIGSAEMLAVVKADAYGHG
                     LVGVAQALAEDARLFGVANLEEALALRAALLHPIIILGPALANERSTIVERGFIPTIS
                     AFEEAKEFSRLGRVAINYKIDTGMGRMGVIESEAAETFKQLAALPNIAIHSVSTHMPV
                     SDADAEYTRDQLARFSKIVRQLRSEVPGDYKVHVLQSAGTLAFNQEPFEIVRAGIMLY
                     GISPLPEFEKLLRPVMTWKTRIGLIRDMPKGSSISYGRTYITPRRMSIATLTAGYADG
                     YPRHLSNRDAAVLVHGERCPLLGRVTMDLMMIDVSKIDNVQAGDEVVLMGRQGDEEIA
                     GSELAEKAGTITWEIVTRIGARVRRVFV"
     gene            11598..11960
                     /locus_tag="VFO30_08300"
     CDS             11598..11960
                     /locus_tag="VFO30_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9419327.1"
                     /translation="MFQIVFNELSAAEISALPKPLQLDLLAEFQILPEDLDNLDAKRF
                     GVIEREGKKLYRYRAKDYRIYFEKTDEGITVHRVLHKNTLRDFLFRSKLPTSEDAQLG
                     KTREFWNLIDEGKRTRKA"
     gene            complement(12123..12362)
                     /gene="rpsR"
                     /locus_tag="VFO30_08305"
     CDS             complement(12123..12362)
                     /gene="rpsR"
                     /locus_tag="VFO30_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9419328.1"
                     /translation="MQPKTAKRRSAFRRANRAMPRRRIDIALEAIDYKNPDLLKKFVT
                     ESGKILPRRVTGMPAYLHRKITREIKRSRSVLLMK"
     gene            complement(12369..12542)
                     /gene="rpmG"
                     /locus_tag="VFO30_08310"
     CDS             complement(12369..12542)
                     /gene="rpmG"
                     /locus_tag="VFO30_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9419329.1"
                     /translation="MPQEIVTLECTEAKALGKPASRYMTTRNKKSPRTPNRLEKKKYN
                     PFLRRHTLHRETK"
     gene            complement(12561..13235)
                     /locus_tag="VFO30_08315"
     CDS             complement(12561..13235)
                     /locus_tag="VFO30_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9419330.1"
                     /translation="MAKSQKKRAAARKPAASRKAKAARKPATKRSTSKTGGRGRISKS
                     TRKNITARPREALIGKNHNERKLDPFTRKQKEKLLQLRDAMVDSMAGVAQDTLRTRAE
                     GSESSAFGMHQADAGSDAYDRDFALSLLSQEQDALYEIDQALKRIELGTYGTCEMSGK
                     PIPRARLEAIPFARFTVECQSQLEKQNKASRVRQSVTSLFGLTEDEGAEGEGEEEVAP
                     VENTKE"
     gene            complement(13719..14513)
                     /locus_tag="VFO30_08320"
     CDS             complement(13719..14513)
                     /locus_tag="VFO30_08320"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9419331.1"
                     /translation="MRRYIDIYSIMLRNSLIREMSFKANFLLWMVVEVLWFCGQIFFF
                     SIIFGNVDRIGDWTKWEVVLLIGTHQIISQLFQAFFFVNVAAVPELVRTGKLDTLLVL
                     PVDSQFAVSTKQFGLDSLINAALGAAVVCVALARLKFVPSVSSTLLYAVALAVGVSVH
                     YSIMLSLAAVSFWIVRAQGLVYGYFNFLTIARYPDVIFPKLFRFIFGWIIPVVIVANI
                     PARLLIKPLGQPAQLMMHLVLAAVIVFSLSRAFWHFALRHYSSASS"
     gene            14560..15735
                     /locus_tag="VFO30_08325"
     CDS             14560..15735
                     /locus_tag="VFO30_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9419332.1"
                     /translation="MRRFLPVATVVVGLFILSFVVRQCRGGAGGNYQTAAITRAPLTQ
                     AVTATGTLNPVVNVQVGSQVSGNIAKLFADFNSVVKAGQVVAQIDPAIFQATVTQAEG
                     DLASAQAALELARVNAQRTQELFAKKNSSQADVDQAIATLHQAEANLKIKQGALDKAK
                     ADLEHCTIVSPIDGIVISRNVDVGQTVAASLQAPVIFAIANDLTKMQIDSNVAEADVG
                     VVAVGQDVDFTVDAFPAQTFHGKVVQVRNAPITVQNVVTYDTVIGVSNPELKLKPGMT
                     ANVSIIVEHKENVLQIKNAALRYRPAEASTATSTMGRARPSGGGRERRGARTVYVFRG
                     SRSTPVQIKTGISDGIVTEVIEGLREGDRVVTAELAAKETAPSPAGNPFSGGPRRFP"
     gene            15848..16597
                     /locus_tag="VFO30_08330"
     CDS             15848..16597
                     /locus_tag="VFO30_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9419333.1"
                     /translation="MNNGALVRLIDVEKTYHTGEVEVHAVRGVSIEIHNGEFVALMGA
                     SGSGKSTLMNILGCLDRPSAGHYLLDGEDVSALNRDRLADIRNRKIGFVFQNFNLLPR
                     TSARENVELPLLYGRHRLTNPQLHEKADRVLTSVGLAGREDHHSSQLSGGQQQRVAIA
                     RALINDPEIVLADEPTGNLDSRTSVEIMQMLQQLNERAITIVMVTHERDIAQYARRNV
                     VMRDGVVLDDFAVQQRLNAAIELGKIARIDK"
     gene            16617..17834
                     /locus_tag="VFO30_08335"
     CDS             16617..17834
                     /locus_tag="VFO30_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9419334.1"
                     /translation="MRVGATVNVAFRALRRNKMRSVLTALGIIIGVAAVIAMVGIGNG
                     AKAQVEAQIASLGENVILIFSGSTTASGIRTGWGSAGTLKIEDAEAIRREVPGAIAVS
                     EEVVSTAQVAAGNQNWFTRVFGESADYFDIRQWSLADGAPFTPQDVRSANKVCVIGRT
                     TATQVYGNEDAVGQILRIKNVPFTVTGVLTPKGLSTQGVDQDDVVVMPYTSAMKRVIG
                     GTTLRNINVQMADARQIAAAQQQIIGLLRQRHSIRPGRDDDFTVRNQQEIAEAATATS
                     RVMTLLLGAIAGVSLVVGGIGIMNIMLVSVTERTREIGVRLAVGAHGRDILTQFLIEA
                     VTLSSVGGIVGIVFGIGASKILSIYAHWPTLISIGSIVIAFLFSAAVGVFFGFYPARE
                     AARLDPIEALRYE"
     gene            17878..18243
                     /locus_tag="VFO30_08340"
     CDS             17878..18243
                     /locus_tag="VFO30_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9419335.1"
                     /translation="MAEHSKFEKLVAEAKKNITEISPEDAAAKMQDHAAVIVDVREKD
                     EWDEEHIPHAIHLSRGTIEFDIEEKIPDPNTVIICHCGGGGRSALAAESLQKMGYKNV
                     RSMAGGFKAWKAAGLPTAK"
     gene            18383..18613
                     /locus_tag="VFO30_08345"
     CDS             18383..18613
                     /locus_tag="VFO30_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET9419336.1"
                     /translation="MKTVLISEFKAKCISLLKNVQKTKQPLVVTLRGEPIARVEPIVP
                     EKKQVRLGALKGWMEIKGDIVSSDMEKDFLVQ"
     gene            18610..>18976
                     /locus_tag="VFO30_08350"
     CDS             18610..>18976
                     /locus_tag="VFO30_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET9419337.1"
                     /translation="MKYLLDTHVWIWSQEDPGRLGNRTRRELADLANERAVSPISTLE
                     IARLLHLGVLHLKHAFTEWRTLSFAHLHATPAPFTHEIATEAYELPGNFHKDPIDRVL
                     VAAARIAACQLEVMRRLGSR"
BASE COUNT         4107 a         5232 c         5551 g         4086 t
ORIGIN      
        1 cctcctcctc cgagccccac gataacttga atcgcctcag accctcgccc tccatcgagg
       61 ttgcgccgag atggagcgtc tccgcacctc cttgcatcag ggatttaata ccctcccaca
      121 ttgcaaggtt gtttggacgt aagttttgaa aggttaggtc ggacgccgca aatttgtaaa
      181 cggccctttt cccatagatg aaaaagactg ccgcacccac tggacgggct tccttttgca
      241 ccatcaccat aaaaccgagc ccgggttcga tgacgctgtc gtagacacgt aagaagtatg
      301 acaacggcag tggcggaagg ccttgtcgct ttcgtgtcag ggtatgaagc cgataaaatt
      361 tgaggacagc ctcgtgactc gaaaccaagc aaacatttaa gccgcttctc tctgctttgc
      421 gaatggcgcg acgcaccgaa ctagcaaaac gggaaaacac aacttctatg ccgctccgca
      481 aatcgatgtt atggccacgt aacgtcacca gtgcaggggc aaccgggtgt agcgagtttc
      541 ctccgcaccg caattcgaag tacttccagt tccgctgccg actaaggctg ctgagagcat
      601 gcaaaattgg agagatatcg ctgccttcga agatcagtgg gttgcagcaa tcaatgaagg
      661 ggaggcaaac tcctcttcgc ccggttacgt gactcgatat ttccatcagt gggaggaggg
      721 ccgaaagatt gctcgcgcga gttaactcta ggtaaactgg ccggtgaccg aaggttgtaa
      781 ccaacacttt ggcccatgtc gcagtgtgaa aaatagttga atcagggtgc gagctgacca
      841 aattgtccca ttcactatcg ccaagcggat caactattct cacgatcgaa gctgctggca
      901 taacctgacg actaatcatg agaaggtgcc cgcttgagtt gctggaagag ccggcgtttt
      961 tcctatctta tgcaaacatc gcgtcttcgg atcggtaaaa caatgacgga gctctccggc
     1021 cagcgcaatc ggccgctggc gcgaaatcga tttttgaact gcagcgcgcc gccttgaaaa
     1081 taacgataca aaaaagttcc gccagttgtt tcccaaaaat ctgtaatgtc tacacgcgct
     1141 ggcccaatgc tttctcgaac tcgacggccg ttggtcgtgg cggaggcgca ttttagtgcg
     1201 gtagcgatcc tcgagccgtt gacgggcagt ctgaatatag gcaatctcaa gcagactcgg
     1261 catggtcgca tcttccacgg gcagagttgt tttgcctatg gtgagcttga aatgaaaatc
     1321 ttttcgaccc tcaagagttc ccataaaata ccgcgtaaga ttctatctca gtaggggtat
     1381 ctatcctaga aattccgaca agggcacggt gattgttgta ggccgagatg aaatccactg
     1441 catctacttc cattttgatg cttgatggtg aatggagagg ggcgtcgcgg atcttgcact
     1501 gcttcgggcg tgtaccccgt cttcgaacac acgtcctttc gcacgtacct gacgttgcga
     1561 gccggttctc tcgttattgc gcagccttcc acctgcgtcc cgcggacagc acctgggagc
     1621 acggattatt gcgagccgtc gagaatattg ctgccagtaa caagattgat gtcatcctgc
     1681 cggtcgccct ggatgcgacg gcatttatcg ccaagcacgc gacacgttta gctcgccttg
     1741 cggcgttgca tcctgttcct gacgcgaaca caattgagac ctgccgagac aagtgtctct
     1801 ttgcacagtt tgcagcaaag catgcgatcc ccacgccgca gactgtgtca tttatcggca
     1861 acgcgcgcga tgacctcgga gtgtttgagc gagggactcc cgtactattg aagccgcgta
     1921 tctccagtaa cggccgcgga ataatctcgt tcgcggacaa gcgcgaggcc gagctcttct
     1981 tggattcgca ccccgaactc cgaagccagt ttgtcgccca accgaggttc gcgggccagg
     2041 acttcggctg taatgtgctc tgtctgcacg gcaacatcgt tgcctacact attcaaaagg
     2101 cggtatttcg cccgaggcag cccttcggag ttgcggctgg aatcgaaatc ctgcacaacg
     2161 atgaagtgtt ggaaatctcg cgtcatctgc tccgagccct gcgctaccac ggcgtcgcca
     2221 atatcgactt acggtatgac cagcgagagc gccggatcaa ggttctcgag ctcaatgcgc
     2281 gatactgggg tactctgttg ggctcgcttt tcgcaggagt aaatttccct tatcttagct
     2341 gcatggcggc gttaggccag cctatcgatt ccccggaata ccgtcacatc caatatttca
     2401 gcgcctgggg acttgggcag caatggcttg gcgcattgcg cgggattgat tggccacctc
     2461 gatcgcctgc ggccagcgtt ttccgttatt tttgcgcgga cccgcttcct gatttgatgt
     2521 cgccgttttt gcggcgaccc ccaccacctt gaaagtcgct aggtctgttc tcaacgcctc
     2581 cactcatttt tttcactcgt cctatccggg taaaacggtg cagttatcgg agctactgcg
     2641 ccgaggatga agaatgtgat gcagagatca gcttatttac ttcggcagca aatttttcca
     2701 cgccgcccga tatataacca aaccgcccta gttcggcacc atcggggcgc aacaccacaa
     2761 ccgtagggaa ttgttcgatg tgatatttga ttgcaagatc ccgattctgg cgcaagactt
     2821 tcgcgtcgcg ctcttgggtt tgataaaaat gtattttgca gagtagaaaa ttgtatttcg
     2881 agttgtcggc aaaagcagct tgatcgaaca cttcccgatc gagctttagg ctcgagatgc
     2941 tccaatccgt gcccaaaaac gcaaggagaa gaggacgatt actctgcttg gcaagagcca
     3001 tagaggaagg aaaatcggtg tcatcacttg cgtcgcaggc cagaatgcta ccatcgagct
     3061 taacaaataa taatccaaga agcgccacgc tgatgagtcg catcatgtca acgaaaacaa
     3121 tcatgcgcgt gcgcgagtgt caactagctc tgcttaaggt atgggtaacg cttgtcgctt
     3181 tgaacggcgc gtggctgtgc gatttggcaa gagcagaaca gatagaaact gtggcgcaag
     3241 tggcgaggcg aatcgacaca agacttcagc agatggagaa tatggcagcg gacgacacgg
     3301 cgactgcact ggcgcaatcg tttttgacgg acctcgacct gttaaaacgg caatcgatca
     3361 tggtgtttgg cgaacgcgag aagcgtttgc aagatgcgag cgataatttg cgaagaggca
     3421 ttgccagttg gagtacagag gcggtcggga acccgaccag acgtcgaatc gtgctggctg
     3481 atatgcgtcg aacgtgggcg gaaatgaagt cacagtggcc gactgaggca ctggagattt
     3541 taccaccact gtggtcttgt ccgatgcatt cggaattaat tgagtccgca gctggaacat
     3601 gtccggtgtg cggaatgccg ctggaaccaa tctatgtcac gcagccacaa ctttccactg
     3661 acccgataat ccgtgccgca atctttccga aaacaccatt acaggttgga agaaaagcgg
     3721 acttgcgact tcatctctta tttaacaaag atggtcgtcc agtcgaactt tccgatttag
     3781 aggaggcgca tacccgtaag attcacctgc tgatcgtcga cagcagcgaa accgactatc
     3841 atcacgagca ccccgagcca gtgggccagg gagaatactc gtttagcttc actccggcac
     3901 gggaaggaat ttaccgagtt tgggtagact tgaagccggt gctaacgcat atccagcagt
     3961 attcggtaac ggacattgct accgccgggg cgccatcagc ggaccctcct gggaatgagc
     4021 ctgaaaaccg gcacacggag atcgacggct ataagttcga tctagcgttt gataggcctg
     4081 tggtccggga gaaggaaacc gcagcgggta ctttgcatat aacggggccg gatgggggac
     4141 cctgcaaacg cttagagata gttatgggcg cattcggtca ttttgttggc ttttgtgaag
     4201 atttcttaac agtgcttcac gtccatcctg ttcaggctgc taccctaacg ccggagtcga
     4261 tgggcggacc agacttgccg ttttatttcc gatcaatcga gccggggaca gttcggctat
     4321 tcactcaggt taaaatcgac gggaaagatt tctttcctcg gtttgtccta aaagttcagc
     4381 cgttacgcga tgcagcggct gagtgaaacc ccgaagggct tgcactttat tacccttgcg
     4441 catttccgct ctgtcctact tctgcccatt gctagaaccg cgcgattttc tggctgcttt
     4501 gatggtcact ggacggtaat ttgagctgta gcgggaagac ttgttaggtt gcgagtgtca
     4561 gtagctgtat aggtgaagct atcgttgccg atatacccga tgtccgcggt gtattgagcg
     4621 gtggcagtta agccgttctg ggatgtaatc gacaccgtcc cgtgcgccgg cgccgaggcg
     4681 atggcgaaag tgagctgcgt cgttgatgcc tgcataatgt agcagtttgt tgatgttctg
     4741 ctcggtttgc agctcgcgct caacctggcc gggacgtccg gaccgacaac actccccgaa
     4801 aaattgatag tgctcgggac accattgagg cacgtcgcag cagtggcatt ggaaatcgcc
     4861 ccagggacgg ttcgcgtctg ttggacggtg ttgttggagg tgtccgattc gtccatgtag
     4921 ccgaagggat tcacctgacc ggtgagtgtg taatccccag gagcaagacc ggtgatatcg
     4981 atccattgat ccgctgtctg cgacggatag tagtctgctt ttcctggcga gattcccatg
     5041 tggacgaacg ccgcgtttgg atcagcgtga gcgcaccacg tgtcgggtcc gacgccgatg
     5101 tatcctggtt tgaaccaccc ggagccaaat gcacggtagc cgtctagcat acagaatccc
     5161 actcccgcct tcgagatcac ccgcccagcc atcgaataac tgtatgcgcc gttaatgtga
     5221 aagtggttat gaccgggtag ctttacgaat ctcatatatg ccccggtcga tgaacgatct
     5281 tccaccgttg ctactgaagg gtcaggagaa gtaaaaaaat ttgggtcagg ctgggttgaa
     5341 gggttaccgc cgacccagat gacctgataa gtcgtcgcat tggcaccctg ctgatagaga
     5401 tcgagcgtac cgccctgatt cacgatgacc gcatcgaacc gatacaggag cttacctggt
     5461 ttctggtagg catcaacgta aatcggagcc acctgcttag gcttagtcgt cggagatgtg
     5521 ataaggagcg ggacaaggtc cggcttgtcc gatgtaggcg tagcggtact ggcaagcggc
     5581 accacattag ccggataatc agatgggtca tccttgcaca tgcaatcttc atcctcgtcg
     5641 tcggacttct ttccggcgcg tgcggtattc ggcgctgccg cgctgtgaac gttcggtcgt
     5701 gttcgcgcgg ccatgctgcc gacttgcgcc ttagcgggaa cggctcggag caaaccaaaa
     5761 gcgatgaaaa ggagtgaaga aagtaggaag aatgagacca gaatttttct cataggtgag
     5821 acatcttttt acactggtct cagcttggcc gacaagcaaa aaatacggcc tatgcgaaaa
     5881 aattattcac aactcattga tggctaagtt aaacaacgcc aatggtaaac gcgatagaag
     5941 tcggcgaccc tgtgcataac attggcaatg cagccggcta aaccgagccc ccttggggag
     6001 cggattcgtt agggcatgaa cacgcggttg cgttcgcccg cgattattcc gtggcgctcg
     6061 ggcggttcca gcttgccgaa gatgactccc gcaagggcga agcggggccg gctacttctg
     6121 taaatcacta aatcacttct cacttgctag aagcgcatcc ggggtgactc aaacagccgc
     6181 ccctcggatt aggaatgcga tgctctatct ggctgagcta cgggcacaag tgggatatgt
     6241 cgccccggct gcaaaggaaa gcccagccca aaaggcagca tcgttaaatt atgaatcgag
     6301 aatcgcgaat cggactgtct ggcggtgtaa cagtgtaacg aatctctggc ccgaagcgcc
     6361 tcagtcgcgg atgcgatgct tcaactccca gacgcgcaag gcggcgagca gaagagcctt
     6421 tgtgcgggtg aggctgccga cggccgccat ttcgcgctca ctgtcttggt gtgcgctgcc
     6481 cgcggctttg ccttttccgc gcactttccg tttcgctttt gagttgcgag cccggcgcgc
     6541 gggcgacccg ctcacggatg cattgccagt gcggctcatg atgccggaag tttgagcagc
     6601 ttctgagcca ccctgggtgt tctggccggc aaggagagcg tccagtgcag cattgccgct
     6661 ttcaaaagcg cttgtgtcag cggggcttct tcggcagttt gccacacaat gacagatcgc
     6721 aagactgggt gtctgcccgc tttcctgttc gtcgcccttt gcctgagcat gttcgtcaac
     6781 ttgctgctgg ttgtggcgtc gttgcaccgg ttcgggcgtg tcgttggtac agaagagccg
     6841 ttgcctcggt tccgcgaagt gctcgtgcaa cgtggcgcac ccgcgacgtt cgacaaggtc
     6901 gccgtaattt tattgcgcgg cttgatcagc acggcgattc cgggcaacac caccgattca
     6961 atggtcgatg atatgcgcgc ggcattgcag caggcgcgcg acgacgatcg ggtgaaggcg
     7021 gtcgtgctgg agattgattc gcccggcgga gaagtcaccg ccgctgacgc gatttacaac
     7081 gcggtggtga aaacgcgcgc gcgtaagccg gtggtcgttt acatggattc gctcgccgcg
     7141 tcaggcggtt actacatcgc ttgcggcggc aaattcctga tggcgaatga aacgaccatc
     7201 accggcagca tcggggtcat tatccagacg ctgaattacg agcagctttt taataaggtc
     7261 ggcctcgctt ccgttgtttt caaaagcggc aaattcaagg acatgctcaa tggcgcgcgg
     7321 ccgatcacgc cggaggagcg cgacctcgtc caaagcttca tcatgaagac ttacgacaag
     7381 tttctcggaa tcgtggcgag cgagcgcaag ctgccgggcg atctgcttcg caacacgatc
     7441 gccgacggcc ggattctttc gggtaaagac gcctacgata acaaactcgt cgacgggctg
     7501 ggccaattag atgacgcatt cgccaaggca aaagaactcg gcaagtcgcc cggcgctaag
     7561 atcgtcaagt acgggccgcc cttcagcctg agccggtttc tgcgcgcatt cggacagagc
     7621 tccacgatcg agctccagct gccgaagcag ctcgtgccac aattggagag cgggcgcgct
     7681 tatttcctgc cgagttacta cgctccgtag ccgcggcggc tcatgtggtc ggacatgctc
     7741 tggacgtcgg ctggcgtcac gaatgttttt gcaattcttt tttccatatt tctcctcctc
     7801 gttggcgctt gcatttacct cgccctcatc ctccaagtca tagcacgtcc atcggagcgg
     7861 gcggttgagg cgaccaaaac attcggtttg cccgaggcga tctttgcaac gtcgttgacc
     7921 ctgtttcttt tgctcggcgc ccttggatcg ctcaccagcg gcacgaagca aatcaccgac
     7981 cgggtgcttg ccgagaacct ccttttcacc ctcggcatcg tggttctcat caccggtctt
     8041 ttgaaatttc gcggcttcaa tgtgaattcg ctagccgggt ttaccaaaac cagtttcgcc
     8101 cacgcggcac tgaccggcgc tgctctttta ctcgcggcct atccacttat cgctttcgcc
     8161 gatgcgatca cgcagcgtct tcttggcagc ggttcgagca aacaaacgat cgtcgagctt
     8221 ttcaattcct cgcgcacaat cgaccgccgg atcatgatta tcattttcgc tgttgccatc
     8281 gcaccggccg ccgaggaatt cctgtttcgc tttttctttt acggtgtgct gagacgatac
     8341 ctcggccggt ttttgggcct tgtcttaaac tcgctgctct tcgctgccgt ccacgcgcac
     8401 ttgccctcgt tcgcgccgct ctttgtgctc gggagttgtt tcaccatcgc ttacgaatgg
     8461 agcggcacca ttctggtctc gatgacaatg catgccttgt tcaatgcggt ttcgctgacc
     8521 gtgctcgcct ttcccgagtt gttcccgcaa tgaaactgag ccgcgtcggc gaagaccggc
     8581 tgctcgccgc gcttttagcg tcgctgcgcg ttgccaaatc agtcgtcgtc agcgccgggg
     8641 atgattgcgc agtcgtgaaa attcgcgcca gcggcgattt tctggttctg aaaaccgatt
     8701 gcgtcgtcga gcaggtgcac ttttcttcct cggccaagcc aaccgatatc ggttggaaag
     8761 caatgatgcg gccgctgagc gattttgcgg ccacatctgc ccttccgcaa ttcgcgttag
     8821 tcacgctgat tgtcagccgg gcaacggaag cggaatgggt taaaaaaatc tatcgcggat
     8881 tgagccgcgc tgctgcgcga tttggcgtca gcatcgtcgg cggcgaaacg agcagtacgc
     8941 cagggccggt cacgatctca atcggcgtcg ccggttgcgt ggaaaagaac cggttcgtgt
     9001 cgcgccgcgg ggcgaaagcc ggcgacgatc tgtttgtgac cggccgtctc ggtggcgcgc
     9061 tgcggcacag gcatttgcag tttgttccgc gaatccccga atcgcgctgg ctcaccgaaa
     9121 atttttcaat ccacgcgatg atcgacctaa gcgacggtct cggcaccgat ctgccgcgat
     9181 tagcgcgtgc aagcaaagtc ggtttcaata tcgacatgca aaacctgccg ctcagtagcg
     9241 gctgtaacat cgacagcgcg ctttccgacg gcgaagatta cgagctgctc ttcgccgttt
     9301 cgccgcgcgc ccgcaaaaga ttgcaagaaa ggtggcgcaa aaaattcccg aaactgccgt
     9361 tgactcgaat cggctctctc aatcggcagt cggcagtcgg caatcggcaa ttgcttggtg
     9421 gctacgttca tttccaatag cccggcggaa acggaagcct tcggccgcga attcgcgcgc
     9481 aagatcgaca agcgagatgt ggtcgcgttg caaggcgagc ttggcgccgg caaaacgcaa
     9541 ttcgtgaaag gcttcgtcgc cggaatcggc agtaacgccg aagcgacgag tcccacgttc
     9601 acgctcattc acgaatacag cggcggacga tggccagttt atcatatcga tttctttcgc
     9661 ctggaagatc gacaatcagc tgcgcggctc ggcctcgacg attatttttt tggggacggc
     9721 gtttcagtca tcgaatgggc ggacaagttt cccgagttca ttcccgactc ggcacgttgg
     9781 atcttgtttt ccacgaaatc cgaaacgcag cgtgccatca cgttaaggtg aaaatcctcg
     9841 cgctcgagct ttccaccgcc cgcgggagtc tcgcctattt gagcgacgct gccgagtttg
     9901 cgcgcgactg gccgcacgag cggaaaaact cggccgcgtt tttcgccaac ctcgccgacg
     9961 tgcaaaagca gttcggccta ccggagagaa tgatcgtcgg gctcggtccc ggctcgtacg
    10021 ccggaactcg gatcgcgatc tcagccgctg tcggcctgca gttcgcctcc ggcgcggagc
    10081 tggtcggttt gccctcaatc tgcgcggtgg agtgtgacgc ggtcgaatac tgcgtcatcg
    10141 gcgacgcgcg acgccagtcg tttttcttcg cgcgcatttg cgcaaacgag ctggtcgaag
    10201 gcccggaact gctcggggaa attgaattgc gcgcgaagat ggagacgctg gacgtgaaga
    10261 tgtcgatctt cgcgtcggaa gaattgccgc agtttccgcg ggtgcaggtt cgttatccct
    10321 cggcgcgcgt gctggcgcgg cttgctcggg acgcgaatcg cgggtttgtt ttgccgccgc
    10381 tcgagccgat gtatctgcgc gagccgcaca tcacggttcc aaaatcgaca tgaccgatca
    10441 atatcgctgc tgggccgaga tcgatcgcag cgcgttacga cacaacgccg cgatcgtgcg
    10501 cgagcgcatc gggtcggccg aaatgctggc ggtcgtcaaa gccgacgcct atggacatgg
    10561 gctggtcggc gtcgcccaag cactcgccga agacgcgcgc ttgttcggcg tcgcgaactt
    10621 ggaagaagcg ctcgcgctgc gtgcggcatt gctgcacccg atcatcatcc tcggcccggc
    10681 actggccaat gaaagatcga caatcgttga gcgcggcttc attccgacga tctcggcgtt
    10741 cgaagaagca aaagagttca gccggctcgg gcgtgtcgcg attaattaca agatcgacac
    10801 cgggatgggg cgaatgggcg tgatcgagag tgaagcagcg gagacgttca aacagctcgc
    10861 ggcgttgccg aacatcgcta tccacagcgt ttcgacgcac atgccggttt cggacgcgga
    10921 cgcggaatac acgcgcgacc agctggcgcg gttcagcaaa atcgtccggc agcttcgctc
    10981 tgaagtgccc ggcgactaca aagtgcacgt gttgcaaagc gcgggcacgc tcgctttcaa
    11041 tcaggagccg ttcgagatcg tgcgcgccgg catcatgctc tacggcatct cgccattgcc
    11101 ggaatttgaa aagcttttgc gtccggtcat gacgtggaag acgcgcatcg gtctcattcg
    11161 cgacatgccg aaaggaagct cgatcagtta tggccgcacc tatattacgc cgcgccgaat
    11221 gagcatcgcg acgttgacgg ctggttacgc cgacggttac ccgcggcatc tttcgaatcg
    11281 cgatgccgcc gtgctggttc acggtgagcg atgtccgctg ctcggccggg tgacaatgga
    11341 tttaatgatg atcgacgtga gcaagatcga caatgtgcag gccggcgacg aagtcgtgct
    11401 catgggccga caaggcgacg aggaaatcgc gggcagcgaa ttggcggaga aggccggcac
    11461 gatcacgtgg gagattgtga cgcgcatcgg cgcgcgggta cggcgggtgt ttgtttgatc
    11521 gagaatcgag aatcgtgaat cgtgtggagt ggtctgacga tgtaacgatg taacggttta
    11581 acgaatcccg acatcccatg ttccaaatcg tcttcaatga actgagcgcc gcggagattt
    11641 ccgcgctgcc aaaaccattg cagctggatt tgcttgccga gttccagatt ctgcccgaag
    11701 acctggacaa tctcgacgcc aagcgcttcg gcgtgatcga gcgcgaggga aaaaagctgt
    11761 accgctatcg ggcgaaagat taccggatct acttcgagaa aaccgacgag ggaatcaccg
    11821 tccatcgtgt cttgcacaaa aacacgcttc gcgatttcct gttccgcagc aaattgccga
    11881 cgagcgaaga cgcacagctc ggaaaaacgc gcgagttctg gaacttgatc gacgaaggca
    11941 agcgaacgcg aaaagcctga cagcgacggg aacgccccaa cgctcaacat cgaacgtcaa
    12001 acgtcaaatg cggaagctgt agcggcattg gtgtcaaacg cccacccgat gacggaccag
    12061 gctttgagag atccctcgac ttcgctcggg atgacaaatt cggcgcagac gcgtcaaatt
    12121 tgtcacttca tcaagagcac cgagcggctg cgcttgattt cgcgggtgat cttgcgatgg
    12181 agatacgccg gcatgccggt gacgcgtcgc ggcagaatct tgccgctctc ggtgacgaat
    12241 ttcttgagca ggtcgggatt tttataatcg atcgcctcaa gggcgatgtc gatgcggcgc
    12301 cgcggcatgg cgcgattggc gcggcggaag gcggagcggc gtttggcggt cttgggttgc
    12361 atcggccgct atttggtctc gcgatgcagc gtgtgccgac gcagaaaagg attgtatttc
    12421 tttttttcga gccggttcgg cgtgcgcgga ctttttttgt tccgcgttgt catataacgc
    12481 gaagcgggtt tgccgagcgc cttcgcttca gtgcactcga gcgtgacaat ttcctgcggc
    12541 ataagatcga caatcgaccg ctactctttc gtgttctcga ccggtgcgac ttcttcttcg
    12601 ccctcaccct cggccccttc atcttcggtc agtccgaata gcgaagtgac cgactggcgc
    12661 acacgcgaag ctttgttctg cttctcgagc tgtgactggc attcgacagt gaaccgcgcg
    12721 aagggaatgg cttcgagtcg cgcgcgcgga atcggtttac ccgacatttc acaggtgccg
    12781 taggtgccga gctcgatgcg cttgagcgcc tggtcgattt cgtaaagcgc gtcctgctcc
    12841 tgcgagagca agctgagggc aaaatcgcga tcgtaagcat cggagccggc atcggcctgg
    12901 tgcatcccga aagccgacga ttcgctgcct tcggctcggg tgcgcaaggt gtcctgggcc
    12961 acgccggcca tggaatcgac catcgcgtcg cgcaactgca gcagtttctc tttctgtttg
    13021 cgcgtaaagg ggtcgagctt gcgctcgttg tggtttttgc cgatcagcgc ctcgcgcggc
    13081 cgagccgtaa tgtttttgcg agtcgatttc gaaattcggc cgcgtccacc ggttttcgat
    13141 gtcgatcttt tggttgccgg cttgcgcgcc gctttcgcct tgcgtgaggc ggcgggtttg
    13201 cgtgcggcag ccctcttctt ttgtgattta gccatgatca ggacgcgcct gcggttgtgc
    13261 ggcgcgaaaa acgaagggcg taattaagaa catttagcca aacgcgcaag cggagatttc
    13321 acgcgaaaaa cagcgcggag tttagggcgt tcggctctgt tgttggagcc aaattcgccg
    13381 cgatacttgg ggcaaagcat tcaaatcgcc acagaccgta acgccaatgg cgggaccttg
    13441 accgactccg aacgcgcgca aatcaatgag gagctggacg acattggtaa gcgcattcac
    13501 gcgcagaaac acgacggcca aacgcagaat ccgtagtcgt tcttctcgcc agcgccagat
    13561 tcacacacgg ttcaaaatgc cggaacgaaa gatccggcgt tttgtctcta ttcgaaacat
    13621 agcctggtcg gacgacctcg agctgtgtcc agactcagcc tagctagaga ttcatcgagt
    13681 tggctcggaa tgacgaggtt gcgctcggga tcctaaactt acgagctggc gctggaataa
    13741 tgacgcaggg cgaaatgcca gaatgcgcgc gacagggaaa agacgatgac cgcggctagc
    13801 acgagatgca tcatgagctg cgcgggctgg ccgagcggtt taatcagcag ccgcgccggt
    13861 atgtttgcca cgatcaccac cgggataatc cagccgaaga tgaaacggaa cagcttcggg
    13921 aaaatcacgt cgggatagcg tgcgatcgta agaaagttga agtagccgta gacgagacct
    13981 tgtgcgcgca cgatccagaa gctgacggcg gcgaggctga gcataatcga gtagtgcacg
    14041 cttacgccga cggccagcgc gaccgcgtag agcaacgtcg agctgacact gggcacaaac
    14101 ttaagccgcg ctaacgcgac gcagacgacc gccgcaccga gcgcggcgtt gatcagcgag
    14161 tcgaggccga actgcttggt cgagaccgca aactggctgt cgaccggcaa aaccagcaac
    14221 gtgtcgagtt tgccggtgcg caccaactcg ggaacggcgg cgacgttgac gaagaagaac
    14281 gcctgaaaca gttgcgaaat gatctggtgc gtgccgatga gcaggaccac ttcccatttc
    14341 gtccaatcac cgatccgatc gacgttgccg aagatgatgc tgaagaaaaa aatctgcccg
    14401 cagaaccaaa gcacctccac caccatccag agaaggaagt tggctttgaa actcatctcg
    14461 cggatgagcg aattgcggag catgatcgag tagatgtcga tgtaacgccg cactcgcttc
    14521 tcctgtcttt agttgtatgg gaagtcaact ttcgtgcgca tgagacgttt tcttcccgtc
    14581 gcgacggtcg tggttggact gttcatcctg agcttcgtcg tccgtcagtg tcgcggcggc
    14641 gctggcggta attaccaaac cgcggcgatc acgcgcgctc cgctcacgca agcagtgacc
    14701 gcgacgggga ccttgaatcc cgtggtcaac gtccaggtcg gcagccaggt ctcgggcaat
    14761 atcgccaagc tctttgccga ttttaattcc gtcgtcaaag ccggccaggt ggtggcgcag
    14821 atcgatccgg caatttttca ggcgacagtg acgcaggcgg agggcgacct ggccagcgcg
    14881 caggccgcac tcgagctcgc gcgggtaaac gcgcagcgga cgcaggagtt gttcgcgaag
    14941 aagaattcgt cacaggccga tgtcgatcaa gcgatcgcga cgctgcatca ggcggaagcg
    15001 aacctgaaaa tcaaacaggg cgcgctcgac aaagcgaagg ccgatctcga gcattgcacg
    15061 atcgtctcgc cgatcgacgg catcgtgatc tcacgtaacg tcgatgttgg ccagactgtc
    15121 gcggccagtt tgcaggcgcc ggtaatcttc gcgatcgcaa atgatctcac gaaaatgcag
    15181 atcgactcga acgtggccga ggcggacgtc ggggtcgtcg cggttggtca ggatgtcgat
    15241 tttaccgtcg acgcttttcc cgcccagacg tttcacggca aagtcgtgca ggtgcgcaac
    15301 gctccgatca cagtgcaaaa tgtggtgacc tacgacacag tcatcggcgt aagcaatccg
    15361 gagctgaaat taaagcccgg catgactgca aatgtgtcga tcatcgtcga gcacaaggag
    15421 aacgtgctgc aaatcaagaa cgcggcgttg cgttatcgtc cggcagaggc aagcacggca
    15481 acatccacaa tgggccgcgc tcggccgagt ggcggtggac gcgagcgacg gggcgcccgc
    15541 acggtttacg ttttccgtgg cagccgctcg acgccggtgc agatcaaaac tggaatcagc
    15601 gacgggattg tgactgaagt aatcgaaggc ttgagagaag gcgaccgggt tgtgacggca
    15661 gaactggcgg cgaaggaaac agcgccgtct ccggcaggta atccattcag tggcgggccc
    15721 cggcgctttc catgactccc agcaacgcaa aagggggatc gagcactccg cgctcgatgt
    15781 taattcaggc ggcgaagccg caaattgtta gcatcgtccg cggagcggcc gatccaccac
    15841 gatcgagatg aacaatggcg cactcgttcg gttaattgat gtagaaaaga catatcacac
    15901 cggcgaggtc gaagtgcacg cggtgcgcgg cgtctcgatt gaaattcaca atggcgagtt
    15961 tgtcgcgctc atgggagcga gcggctctgg caaatcgacg ctcatgaaca ttctcggctg
    16021 cctcgaccgg ccgagtgccg gccattacct cctcgatggc gaggacgttt ccgcgctcaa
    16081 tcgcgaccgc ctcgcggaca ttcgaaaccg caaaattggt tttgtctttc aaaactttaa
    16141 tctgctgccg cgcacctcgg cgcgcgagaa cgtggagttg ccgctgctgt acggcagaca
    16201 tcgcctaaca aatccgcaat tgcacgaaaa agccgatcgc gttctcacgt cggtcggtct
    16261 ggcgggtcgc gaagatcatc attcgagcca gctttccggc ggccagcaac agcgcgtcgc
    16321 gattgcccgg gcgctaatta acgatcccga aatcgtgctt gcagatgaac cgaccggtaa
    16381 cctcgacagc cgcacgagcg tcgagatcat gcagatgctc cagcagctca acgagcgcgc
    16441 catcaccatc gtgatggtga cgcacgagcg cgacatcgcg caatacgcac gccggaacgt
    16501 cgtcatgcgc gacggcgtcg tgctcgatga tttcgcggtc cagcaacggc tcaatgccgc
    16561 gatcgagctg ggcaaaattg caaggatcga caagtgatga gcacgctaca aaaaagatga
    16621 gagtcggcgc gaccgtgaac gttgccttcc gtgctttgcg tcggaacaag atgcgctcgg
    16681 ttctgaccgc gctcggcatc atcatcggcg tggctgccgt gatcgcgatg gttgggatcg
    16741 gaaacggcgc aaaagcacag gtggaagcgc aaatcgcgag cctgggtgaa aacgtcatcc
    16801 tgattttttc gggcagcacg acagcaagcg gaatccggac tggctggggc agcgcgggca
    16861 cgttgaaaat cgaagacgcc gaagcgatcc gccgcgaagt gccgggtgcg atcgcggtga
    16921 gcgaagaagt ggtttccacg gcgcaagtag cggcgggtaa ccagaactgg tttacgcgag
    16981 tcttcggaga atcggccgat tatttcgata tccgccagtg gtcgcttgcc gacggcgcgc
    17041 cgtttacgcc gcaggacgtg cggagcgcaa acaaagtgtg tgtgatcggc cgcacgaccg
    17101 cgacgcaggt ttacggcaat gaggatgccg tcgggcagat tttgcgcatc aaaaacgtgc
    17161 cgttcaccgt cactggtgtg ttgacgccga aaggattgag cacgcagggt gtcgatcagg
    17221 atgatgttgt ggtgatgccc tacacgagcg cgatgaagcg cgtcatcggc gggacaacgt
    17281 tgcgtaacat caatgtgcag atggctgatg cgcgccagat cgcggccgcg caacagcaga
    17341 tcatcggctt gcttcggcaa cgacatagca tccggcccgg acgcgacgac gattttaccg
    17401 tgcgcaacca gcaggaaatc gccgaggccg cgaccgcgac atcacgggtg atgacgcttt
    17461 tgctcggggc gatcgccggc gtatcgctcg ttgtcggcgg gattggcatc atgaacatca
    17521 tgctggtgag cgtgaccgag cggacacgcg aaatcggcgt tcgcctcgcg gtcggcgctc
    17581 acggccgcga cattctcacc caattcctga tcgaagcggt caccctcagc tcggtcggcg
    17641 gcattgtcgg catcgtcttc ggcatcggcg cgtcgaagat attgtcgatc tacgctcatt
    17701 ggccgacgct catttcgatc ggctcgattg tgattgcgtt tctattcagc gccgcggttg
    17761 gcgttttctt cggtttttat ccggcgcgcg aagccgcccg tctcgacccg atcgaagcat
    17821 tgcgctacga ataagttttc tgtagcggcc gctgtcctca gcggcaaatt ctcagctatg
    17881 gctgaacatt cgaagttcga gaaactggtc gccgaagcga aaaagaacat caccgagatt
    17941 tcgccggaag acgcggcggc gaaaatgcag gatcacgccg cggtaattgt cgatgtccgc
    18001 gagaaggacg aatgggacga ggaacacatc ccgcacgcga ttcatctgag ccgcggcacg
    18061 atcgagttcg acatcgaaga gaaaattccc gacccgaaca cggtgatcat ttgccattgc
    18121 ggcggtggcg gacgttccgc tctggccgcg gaaagtttgc agaagatggg ctacaaaaac
    18181 gtccgctcga tggccggcgg cttcaaagcc tggaaagccg ccggcttgcc cacggcgaag
    18241 tagttaatcg aacgctcgtt ctgattggag ccgcatctta gagtggatgc cttctcgtag
    18301 gctacttgcg agctgcggct cgttaatggc gcggctttga accgcggctt gacttagtcg
    18361 attgaccaag taatctagtg acgtgaagac ggtcctgatc tcagaattca aagcgaaatg
    18421 catcagcttg ttgaaaaacg tgcagaaaac aaagcagccg ctcgtggtga cgttgcgcgg
    18481 agaaccgatc gcgcgggttg agccgatcgt gccggagaaa aagcaggtcc gtctcggcgc
    18541 gcttaaaggc tggatggaaa ttaaaggcga catcgttagc tcggacatgg agaaggattt
    18601 cctcgtccag tgaaatacct tctcgacacg cacgtctgga tttggagcca ggaagatccg
    18661 ggacgattgg gcaatcggac acgccgagaa ttggccgact tggctaatga acgggcggtt
    18721 tcgccaatct cgactttgga aatcgcgcgg ctgcttcatc tcggcgtgct gcatttgaag
    18781 cacgcgttca cggaatggcg gacactttcg ttcgcccatc tgcacgcgac gccggctccg
    18841 ttcacgcacg aaatcgccac cgaggcttac gagttacccg ggaattttca caaagatcca
    18901 attgatcgcg tgctggtggc ggccgcgcga atcgccgctt gccagctcga agttatgcga
    18961 cggctgggaa gccgtc
//
LOCUS       DASTDG010000253         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_511746, whole genome shotgun sequence.
ACCESSION   DASTDG010000253 DASTDG010000000
VERSION     DASTDG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511746"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..944
                     /locus_tag="VFO30_08355"
     CDS             189..944
                     /locus_tag="VFO30_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9419338.1"
                     /translation="MAGHSKWSKVKRFKGAIDAKRGRIFSKLSKEITIAVKAGGGDPD
                     ANPRLRSAIQAARAQSVPGDNIERAIKRGTGGASDGAQLEEITYEGYAPGGVAIIVEA
                     TTENRNRTAAEIRSLFTKHEGNLASSGSVSYMFHKKGQITVARDSIDEDRLFELALDA
                     GAEELTTEDDQYVIITSHDQLYAVAEALKRARVATDGQKFTFIPDTTVQVADENVALQ
                     VLRLCDALEEDDDVQNVYSNLDIPDDVLAKLPA"
     gene            1004..2386
                     /gene="rho"
                     /locus_tag="VFO30_08360"
     CDS             1004..2386
                     /gene="rho"
                     /locus_tag="VFO30_08360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9419339.1"
                     /translation="MNEENETPPPVDTEAAAPEQRRFRPHRRRSRRRYYDRADRFANE
                     NSNAQQNNSPGELGAGEAVETLDRSAEGRIRGGEQSAETSAENGEPIQAEPEFGEGIV
                     EISGKGFGFLRDPKRNFVQTPQDIFVTPDIVRRFGLRDGMWIYGETRRGSRGPQLTKL
                     LTINGEDPAKYQALKPFEELTTINPNKRIKLETVPDRYTTRIMDLMTPLGMGQRGLIV
                     APPRTGKTTLLHHIADAVTKNHKDMHVIILLVDERPEEVTDFRRSHPTAELMASSNDS
                     DIKSHTRIAQLAIERAKRLVEAGENVFILLDSITRTARAFNNAMAGGGRTMSGGIDAR
                     AMEIPRKIFAAARNTEEAGSLTIVATALIETGSRMDELIFQEFKGTGNMEMVLDRKIS
                     DQRIYPAIDIFLSGTRREELLLQPWELEKINLIRRGLAGHKPEEAIQRLLMFVKKFPT
                     NVQMLKEIPG"
     gene            2511..2936
                     /locus_tag="VFO30_08365"
     CDS             2511..2936
                     /locus_tag="VFO30_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET9419340.1"
                     /translation="MIMDELLRLPGTKFRFGLDPLIGLLPGLGDTASAFVSALTLVQA
                     ARLGVPKILLARMSLNILTNEIIGIIPGVGDAFSFWFKSNTRNYNLLQRHMTATQRSR
                     NSDWIFVAVVIGLVFLIVCGGLLVSLLVFNELLKLLSRT"
     gene            complement(2923..>3764)
                     /locus_tag="VFO30_08370"
     CDS             complement(2923..>3764)
                     /locus_tag="VFO30_08370"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419341.1"
                     /translation="TALAQACLVFVNEILDGAELTVIAMGKFGGREISYGADLDVLFI
                     GSDDRQPQSLLSALAQPSPAGNLPRVDARLRPEGEKGPLTCSLETYRQYYAGRAQAWE
                     LQALTRARPITGPLANNFMEIARDVWLNAGKEVDLFRKIDNMLQRIRRERGSGSDFLD
                     FKTGSGGSVEAEFLVQALQMRGGIFEPNWRRAIDRLHEAGVLSESDVVRLKRSYAFLR
                     RCESVLRRYDNRAVATLPADVMEQRNFARRMDGQTIEAFTEEYLAARSTIHQIYEERI
                     RCG"
BASE COUNT          845 a         1114 c         1051 g          754 t
ORIGIN      
        1 cgcatccacc gtccgaggtg ggcgcgcttg acaaatcgcg cccgcggcga ttgactactg
       61 gctggtaact cttccgcgcc aaggatcgtc tccttagccg cagcgaatct gtccaatgcg
      121 cggacgcgag ccacgttcgg aatttacgtc aattcgttag gcaagttgtc ttagaaatcc
      181 gcaaatttat ggcgggacac agcaaatggt cgaaggtgaa gcgcttcaaa ggagcgattg
      241 acgcgaaacg cggccgaatt ttcagcaagt tgtccaaaga gatcacgatc gccgttaagg
      301 ccggcggagg cgatcccgat gccaatccac ggctgcggag tgcaattcag gcggcgcgag
      361 cgcaaagcgt gcccggcgat aacatcgaac gcgcaatcaa gcgcggcacc ggcggggcga
      421 gcgatggggc acaattggaa gaaatcacgt atgaaggcta cgcgcccggt ggcgtcgcga
      481 ttatcgtcga ggcaaccacg gagaacagaa accgcaccgc ggccgagatt cgcagcctct
      541 tcacaaaaca cgagggcaat ctcgcctcct cgggcagcgt ctcttacatg tttcacaaga
      601 aagggcagat caccgtcgcg cgcgattcga ttgatgagga tcgcctgttc gagctcgcgc
      661 tcgacgccgg agccgaagag ttgacaaccg aggatgatca gtatgttatt atcacttctc
      721 acgatcagct ctatgctgtg gctgaggcgc tcaaacgagc gcgggtagcg accgatgggc
      781 aaaagtttac atttattccc gataccactg ttcaggtcgc cgatgagaac gtggcgctcc
      841 aggtgctgcg attgtgtgac gccctcgaag aggatgacga cgtacaaaac gtctactcaa
      901 atctagacat tcctgacgac gtgttggcga agttgccggc ttgataattt aaaacggaac
      961 acggttccgc gttgccgaaa caggcgacga atccgattct aacatgaacg aagaaaacga
     1021 aaccccacca ccggtggata ctgaagcggc cgcgcccgag caacgccgtt ttcggcccca
     1081 ccgccgccgt tcccgccgcc gctattacga tcgggctgat cgattcgcta acgaaaattc
     1141 caacgcccag cagaacaatt cgccgggcga gctcggcgcg ggagaagctg tcgaaacgct
     1201 agatcgatcc gccgaaggac ggattcgcgg tggcgaacaa tcagccgaaa ccagcgccga
     1261 aaacggcgag ccgatccaag cggaaccaga attcggtgaa ggcatcgttg agatttccgg
     1321 aaaagggttc ggttttctgc gcgatcccaa gcgcaacttc gtgcaaacgc cacaggacat
     1381 ctttgtcacg ccggacatcg tgcggcgttt tggtctgcgc gacggcatgt ggatttacgg
     1441 cgaaacccga cgcggcagcc gcggcccgca gttgaccaag ctgctcacca tcaacggcga
     1501 ggatccagcc aagtatcagg cgctcaaacc gtttgaagag ctcacgacca tcaatccgaa
     1561 caagcgcatc aaactcgaga cggtgccgga tcgctacacg acccgcatca tggacttgat
     1621 gacgccgctc ggcatgggac agcgcggctt gattgttgcg ccgccgcgca ccggtaagac
     1681 gacgttgctg catcacattg ccgacgcggt cactaaaaat cacaaagaca tgcacgtcat
     1741 catcctgctg gtcgacgaac ggccggagga agtcaccgat ttccggcgtt cccatccgac
     1801 ggcggaactg atggccagct cgaacgacag cgatatcaaa agccacacgc gtatcgcgca
     1861 gctcgcgatc gagcgcgcga agcggctggt cgaagccggc gaaaacgtgt ttatcttgct
     1921 cgactcgatc acccgaacgg cacgcgcgtt taacaacgcg atggccggcg gcggccgcac
     1981 gatgagcggc ggaatcgacg cccgtgcgat ggaaattccg cgcaagattt tcgcggccgc
     2041 acgaaatacc gaagaagcgg ggtcgctcac cattgttgcg accgcgctca tcgaaaccgg
     2101 cagccgcatg gatgagctta ttttccagga gttcaaaggc accggcaaca tggagatggt
     2161 gctggatcgc aaaatcagcg atcagcgcat ttatccggcg atcgatatat tcctctccgg
     2221 cacgcgccgc gaagaactgc tccttcagcc atgggagctg gagaagatca atctcattcg
     2281 tcgcggtctc gccggccaca aaccggaaga agccattcag cgtctattga tgttcgtgaa
     2341 gaaattcccg accaacgtcc agatgctgaa agagattccc gggtgattct ctcacaatcg
     2401 tcgaacttac gctgaattct ccgccagcac gggaagtcga gtgggaagtt ctcccgcccg
     2461 aagaagagca aaggcgcgct cagctcgagc cgatttttcg ctcggtcgcg ctgatcatgg
     2521 atgagctgtt gcgtctcccc gggacgaaat tccgcttcgg tctcgatccg ctgatcgggc
     2581 ttttaccggg attgggcgac accgcttcgg cttttgtctc ggctttgact ttggttcagg
     2641 cggcgcgact cggggtgccg aagattctcc tcgcgcgcat gtcgctcaac attctgacca
     2701 acgaaatcat cggcattatt ccaggcgtgg gcgatgcgtt ttccttttgg ttcaaatcga
     2761 acacgcgtaa ctacaatttg cttcagcggc acatgacggc gacccaacgc tcgcgcaata
     2821 gcgactggat ttttgtcgcg gtagtaatcg gtttggtgtt cctgattgtg tgtggtggct
     2881 tgctggtcag cttgctcgtt ttcaacgagc tgctcaaact gctcagccgc acctgattcg
     2941 ttcctcgtaa atctggtgaa tcgtcgatcg cgcggcgaga tactcttccg tgaacgcctc
     3001 gatcgtctgc ccgtccatac gtcgggcgaa gtttcgctgt tccatcacat ctgccggtag
     3061 tgtcgcaact gctcggttgt cgtagcgtcg caagaccgat tcgcagcgac gcaaaaacgc
     3121 gtagctccgc ttgagcctaa caacgtctga ttcgctcaac actcccgctt cgtgcaagcg
     3181 atcgattgcg cgcctccaat ttggctcgaa aatcccgccg cgcatttgca acgcttgcac
     3241 gagaaactct gcttcaacac tcccgccgct gccggtcttg aaatcgagaa agtcggaccc
     3301 gctgccgcgc tcgcgccgaa tgcgctgcag catattgtcg atcttacgga aaagatcgac
     3361 ttctttgccg gcgttcagcc agacgtcgcg ggcaatttcc atgaagttgt tagccaacgg
     3421 tccagtgatc ggtcgcgcgc gtgtcaaagc ctgcaactcc cacgcctgcg cgcggccggc
     3481 gtagtattga cggtaggttt caagcgagca cgtgagcggg cccttttcgc cttccggccg
     3541 taaccgcgca tccacgcgcg gcaaatttcc cgccggactc ggctgcgcca gggcggacaa
     3601 caaactctgc ggctgccgat cgtcgctccc gatgaaaagc acatccaggt ctgcgccata
     3661 actgatctct cggcctccaa atttccccat tgcgatcaca gttaactctg caccatcgag
     3721 tatttcgttc acaaaaacca agcaagcctg tgcaagtgct gttt
//
LOCUS       DASTDG010000254         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_393740, whole genome shotgun sequence.
ACCESSION   DASTDG010000254 DASTDG010000000
VERSION     DASTDG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393740"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFO30_08375"
     CDS             complement(<1..551)
                     /locus_tag="VFO30_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9419342.1"
                     /translation="MLFSDYHTHPQAHRLQRYTQTLLQPWVDAARKLGLSDIAFTDHD
                     RFHEGVDFDEIDRLREQNPELKIRAGIELDNDPASSAAGRKWIEQNWERLDFVLGSVH
                     FLDRADQMFDTVPAGAAQFEGRNIDDIYADYFRRVREIAASGLVDCLAHLDLIKIHGH
                     RPSAEIGSIVVETLELIRARNLA"
     gene            complement(657..1187)
                     /locus_tag="VFO30_08380"
     CDS             complement(657..1187)
                     /locus_tag="VFO30_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HET9419343.1"
                     /translation="MRRSLAPLVLLAAVATHAFAQEPTPTSAAKKRSWFGRLFHPFSS
                     RSIPQYHDPRLRGLALELLVTPQIVKLSEVRQLQIKLTVTNESNRPVTLDFPTDQRIE
                     IYLRNSAEAILTKWTENHAINDAPGTVLINPHEHVEYNEPIATRELTPNKVYAVDVFL
                     PQYPELRIRQKIMTAP"
     gene            1243..1710
                     /locus_tag="VFO30_08385"
     CDS             1243..1710
                     /locus_tag="VFO30_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419344.1"
                     /translation="MKSLVCTPLVLATAFSFAAAKDRHCTFRVHVEANARDTAVFASS
                     VRAQVSGRQVAIERLPRISERDVVAFYPYNAGPENYGALLQLDDHGRIALDALSIEHR
                     GSLLFVFVNGRAITQMQIDKRVADGKIYIPSGLSAADIDLMKKDWSLLGRRKR"
     gene            1758..2753
                     /locus_tag="VFO30_08390"
     CDS             1758..2753
                     /locus_tag="VFO30_08390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9419345.1"
                     /translation="MATGAEIRHDVVDLALPTDNDALFHGGGPAFYQAIERNYRGVKS
                     TPWEGGQYGFVREPLQTARGIVYTRFHEGIDIRPWQRDARGEPLDTVRAIAGGEVVYV
                     NLAPTHSDYGMYIVIEHRWDGSSYYSLYAHLRAANVRAGQEVARGEQIAAMGYTGTGI
                     NQQRAHLHLELNLMLSRHFESWYETFRKEEPNYHGLYNGINLKGMDIARLYLALKRQP
                     SLTIPEFMADEEIFYKVALPNSTHFDLLELYPWLLREPTRTDIGSWEVSFARSGLPLA
                     VQARAQAVAQPQLTWVKPATIDYRSLTQDYVAGRGKGAHLTKRGAELMQLLIWPR"
     gene            2883..3647
                     /locus_tag="VFO30_08395"
     CDS             2883..3647
                     /locus_tag="VFO30_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9419346.1"
                     /translation="MSSITRRQFLKIGALAAPAALGIDARFIEPTALRVRELPLNNSG
                     NCRFVHFTDLHYKGDAKYAAEIVRAINAVQPEFVCFTGDIVEQRQFADAALGFIREIK
                     APVYGCPGNHEYSSGISFRDCEAAFAATGGAWLMDRNLVLPQHNLEIVGMGIVGLRDV
                     PPLPASRRLLLMHYPRVADHLGDRKFDLILAGHSHGGQVRLPFCGALILPRGVGKYDL
                     GYYKTNGGPLHVSAGVGTYVLPIRLNCPPEITLITI"
     gene            complement(3648..4130)
                     /locus_tag="VFO30_08400"
     CDS             complement(3648..4130)
                     /locus_tag="VFO30_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9419347.1"
                     /translation="MQIVVGIITISDRASSGEYQDLGGPALKEFAQNQAWQVLCEAIV
                     PDNMPRIQETIRSFARQGCGLILTTGGTGIAARDVTPEAIRGIMRLELPGFGEVMRAE
                     SMKITPNAILSRSLAAIVDLSLVIALPGKPSGAVECLGFVAGAIPHAVALVQRLPTSC
                     "
     gene            complement(4161..>4388)
                     /locus_tag="VFO30_08405"
     CDS             complement(4161..>4388)
                     /locus_tag="VFO30_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9419348.1"
                     /translation="CHTIPLSEVKIDIVSSRAAAEVTCTARTTAQTGVEMEALTGVAV
                     ALLTIYDMCKAIDKEMEIGKVRLLKKTKRDV"
BASE COUNT          883 a         1230 c         1290 g          985 t
ORIGIN      
        1 atcgcgagat tgcgtgcgcg gatgagctcg agtgtttcca ccacaatcga gccaatctcc
       61 gcgctgggtc ggtgcccgtg gattttgata agatcgaggt gggcgagaca atcgacgagg
      121 ccgctcgccg cgatctcgcg aacccgccga aaataatcgg cgtagatatc gtcgatgttg
      181 cggccttcga attgcgccgc gcccgccggg acggtgtcga acatctggtc ggcgcgatcc
      241 agaaaatgga cggacccgag gacgaaatcg agccgctccc aattttgctc gatccatttg
      301 cgtccggcgg ccgagctcgc cggatcgtta tccagttcaa tgccggcgcg aattttcaat
      361 tccgggtttt gttcgcggag ccggtcgatt tcatcgaagt ccaccccttc gtgaaaacgg
      421 tcatggtcgg tgaaagcgat atcgctaagt ccgagcttgc gcgccgcgtc cacccagggc
      481 tgcaaaagtg tttgcgtgta gcgctgtagg cgatgcgcct gcgggtgcgt gtggtaatcg
      541 gagaaaagca ttggtggatc gtgctctccg agcgcgatgt tagcctagtc gccgtaggcg
      601 catttgctca acatcacccg cggagcggct gatcaaccgc gtgcacgccg tacgactcat
      661 ggcgcggtca tgattttttg gcggatgcgc agttcgggat actgcggcag gaaaacatcg
      721 accgcgtaga ctttgtttgg cgtgagctca cgcgtcgcga tgggttcgtt gtattcgacg
      781 tgctcatgtg gattgatcag gaccgtgccg ggggcgtcgt tgatggcgtg attctcggtc
      841 cacttcgtca ggatcgcttc ggctgaattg cgcaagtaaa tctcgatgcg ctggtcggtg
      901 gggaaatcga gagttaccgg tcggttcgat tcgttggtca cggtcagctt gatttgaagc
      961 tgccgcactt ccgacagctt gacgatttgt ggcgtgacaa gcagctccaa cgcgagcccg
     1021 cgcagccgtg ggtcgtggta ttgcgggatg gaacgcgaag aaaagggatg aaacaagcgc
     1081 ccgaaccaac tccgtttctt tgccgcggaa gttggcgttg gttcctgggc gaaagcgtgt
     1141 gtcgcgactg cagcgagtaa gacgagtggc gcgaggcttc gcctcatgag cgggtaaaca
     1201 gcaaacgttg caaatcgtgt caaacccttt atcaatcgcg cggtgaagtc gctcgtttgc
     1261 accccgctcg tgctcgcgac cgcattttcg ttcgctgcgg cgaaagatcg acattgcacg
     1321 tttcgcgtgc atgtggaagc aaacgcgcgc gacacagcgg tcttcgccag ttcagtgcgc
     1381 gcgcaggtgt ctggcagaca agtggcgatc gagcggttac cgcgcatctc cgaacgggac
     1441 gtggttgcgt tctatcccta caacgcagga ccggaaaatt atggcgcatt gttgcaactc
     1501 gacgatcatg gccggatcgc actcgatgcg ctcagcatcg aacatcgcgg cagtttgctt
     1561 tttgtttttg tgaatggccg ggcaatcact cagatgcaaa tcgacaaacg cgtggccgat
     1621 gggaagatct acattccttc cggcttgagc gccgcggata tcgatttgat gaaaaaagac
     1681 tggtcactgc tcggccggcg caaacgctag cgcggatgcg gctcctcgcc agcttggctg
     1741 tcgttattgc tctggcaatg gcaactggag cagaaattcg ccatgacgtt gtcgatcttg
     1801 cgttgccgac tgataacgac gcgttgtttc acggcggcgg gcccgcattt tatcaggcta
     1861 tcgagcgcaa ctaccgtggg gtgaaatcga caccgtggga aggtggccag tatggtttcg
     1921 tacgcgagcc cctccagacc gcgcgcggaa ttgtttacac ccgttttcac gagggcatcg
     1981 acattcgtcc atggcagcgt gatgcccggg gcgaaccgct cgacacggtt cgcgctatcg
     2041 cgggcggaga agtggtctat gtcaatctgg cgccgacgca ttcagattac ggaatgtaca
     2101 ttgtgatcga gcatcgctgg gacggctcaa gctattacag tctttacgcg cacctccgcg
     2161 ccgcgaatgt tcgtgccggc caagaggtcg cgcgcggcga gcaaatcgcg gcgatgggtt
     2221 ataccggcac aggcatcaat cagcagcgcg ctcatttgca tctggaactc aacttaatgc
     2281 tgagccgcca tttcgaatcg tggtatgaga cgtttcgaaa agaggaaccc aattaccacg
     2341 gcctttacaa tggcatcaat ctcaagggaa tggatatcgc gcggctttat ctcgcgctaa
     2401 agcgtcagcc ctcgctgact attccggaat tcatggctga cgaagagatc ttttacaaag
     2461 tcgccctccc aaactcgacg cattttgatc tgctggaact gtatccgtgg ttattgcgtg
     2521 aaccgacacg aaccgatatt ggttcttggg aagtttcatt cgcgcgatct ggtttgccgc
     2581 tcgcagttca agcgcgcgcg caagctgtcg cgcaaccgca attaacctgg gtcaagccag
     2641 ccacaattga ttatcgttcg ctcacccagg actacgtcgc cggtcgcggt aaaggagcgc
     2701 atctgaccaa acggggcgcg gaattgatgc agcttctcat ttggccgcgc tgacgctcaa
     2761 gttcgctgca acatttttcc gaattctctg tctaacagtt caaactgttt cctttgatca
     2821 ccggctgaac agaggctgtc acggggtaag ggccgcatgg attatgctcg gttaatgtct
     2881 cgatgagctc tatcactcgc cgccagttcc tgaagattgg agctctcgcc gcgcccgcag
     2941 cgctgggcat cgacgcgcga tttatcgagc cgactgctct gcgcgtccgc gaattgccgc
     3001 tcaataactc cgggaattgt cggttcgttc atttcaccga tctccactac aaaggcgacg
     3061 cgaagtacgc ggccgaaatc gtgcgtgcga tcaacgcggt tcagccggag tttgtttgct
     3121 tcacggggga cattgtcgag caacggcagt ttgccgacgc ggcactcggc ttcattcgcg
     3181 agatcaaagc gccggtttat ggctgcccgg gaaatcacga atactcgagc ggtatttcat
     3241 tccgcgactg cgaggcagcc tttgccgcaa cgggcggtgc gtggctgatg gaccgaaact
     3301 tggttttgcc gcagcataat ctcgagattg tcgggatggg aattgttggc ctgagggacg
     3361 tgccgccgct gcccgcgagc cgacgcttat tgctcatgca ttatccacgc gtcgccgatc
     3421 atctcggcga tcgaaaattc gatctgattc tcgccggtca ctcgcacggt ggccaggtgc
     3481 ggctgccatt ctgcggtgca ctgatcctgc cgcgaggtgt cggaaagtac gatctcggct
     3541 attacaaaac gaacggcggc ccgttgcatg tcagcgcggg cgtcggcact tacgttttgc
     3601 cgattcggtt gaattgcccg ccggaaatta cactaataac gatctaatca gcacgatgtc
     3661 ggcaggcgct gcacgagtgc gacagcgtgc gggatcgcgc ccgcaacaaa tccgaggcat
     3721 tcgaccgcgc cacttggctt cccgggcagg gcaatgacca gtgaaagatc gacaatcgca
     3781 gcgagactac gcgatagaat cgcgtttgga gtaatcttca tcgactccgc acgcatcact
     3841 tcgccgaacc cgggtagttc caggcgcatg atgccgcgga tggcttccgg ggtgacatca
     3901 cgcgccgcga ttccagttcc gcctgtcgtc aagattaagc cgcaaccctg tcgtgcaaac
     3961 gagcggatcg tctcctgaat gcggggcata ttgtcaggca cgattgcctc gcaaagaacc
     4021 tgccacgctt ggttctgcgc gaactccttt agcgccggtc ccccaaggtc ttgatactca
     4081 ccgctgctcg cacggtcaga gatcgtgata atgccgacga caatttgcat ttctaccgag
     4141 ccgcccaacg aacagcgctg tcaaacatct cgcttcgtct ttttcagcaa acgcaccttt
     4201 ccgatctcca tctctttgtc gatggccttg cacatgtcat aaatggtcag cagcgcgacg
     4261 gccacgccgg tgagagcttc catttcgacg ccggtttgcg cggttgtgcg cgccgtacat
     4321 gtcacctcgg cggcagcccg cgagctgacg atgtcgatct tgacttcgct gagcggaata
     4381 gtgtggca
//
LOCUS       DASTDG010000255        14512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1151371, whole genome shotgun sequence.
ACCESSION   DASTDG010000255 DASTDG010000000
VERSION     DASTDG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151371"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFO30_08410"
     CDS             complement(<1..248)
                     /locus_tag="VFO30_08410"
                     /inference="COORDINATES: protein motif:HMM:NF045310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET9419349.1"
                     /translation="MLRKTEYPAALRELISQLRQMPGVGPRSAERIALWMIQARADAP
                     EQIARAINETRRAIQPCKRCGYFTSDEICDICADASRAA"
     gene            complement(253..1503)
                     /locus_tag="VFO30_08415"
     CDS             complement(253..1503)
                     /locus_tag="VFO30_08415"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9419350.1"
                     /translation="MKRPLLTSLIIAVAVGGVIAVLHATRVLLPIERPIAQLVSRDLG
                     ATKSAADFWQYLLTGVLAFGVAAFTLTTPRRGRLGWIVLALLIELAGLAWVCVLYRTF
                     FQPLPSMLAVVLAFAAGERWAAITTRSRTFIARSFFGGRLSKQQVGRLIAGEIPFEDE
                     PQTCDVSIVVCDIANKYDLADGATPAAFAEVTEKFIHRATDLVLEAGGYVEAADGEGV
                     VAIFGFPDRNDDHGNKAVRVALDVVQAFRTTRAQSDGERSGEPDVHVGISSGTVIIAP
                     LHDGERPALITSGEPVELARRFCVANRFYGSRILIGPQTFELTSREIVARPIDFLSGV
                     NSQERHEIYEPLWLAAEAKPEHLARRDCFWNGVVLYREKRWAEAYSEFQKARGPDADD
                     DAPLQLYLRRLEPLALQLMETPSG"
     gene            complement(1510..3120)
                     /locus_tag="VFO30_08420"
     CDS             complement(1510..3120)
                     /locus_tag="VFO30_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9419351.1"
                     /translation="MEKNPPPFPSPEELKAKITEFMKSNFGDRVSVATFGQPDVAETQ
                     VEEKPEKIDKQPFEFDFLPRDIKAHLDRFVIKQDEAKKVLSIAVCDHYNHAKYLRQLE
                     KEDAKRAEQTEYAKQNVILVGPTGVGKTYLIKHIADLIKVPFVKADATKFSETGYVGG
                     DVEDLVRELVHKADGDVNIAQFGIIYIDEVDKIAAAGSLIGRDVSGRGVQTTLLKLME
                     ETEVPVRSMNDLQGQLQAAFEFQRRGKAKRDTINTRHILFVVSGAFEKLKEQVARRVR
                     QGQIGFSAEPVQVMDNQLFQHVTTQDFVEYGFEPEFIGRLPVRVVCEDLDTNDLYKIM
                     KYSEGSLLRQYERAFRAYGIEISFEDDALRLLAEAAAKEKTGARGLLTVFEKLFRDYK
                     YYLAGSGVSQLRVTVDLVREPQRVLERLMAEGNRHEAKALEAGVRAFADKFQREHGIE
                     IVFDEPATARLVERAQSERMSMADLCAHLFKDFQFGLSLICKNTGQKRFPLDRAAVDA
                     PDKFLSELVVQSYYPERAAMVADAGAHA"
     gene            complement(3234..4028)
                     /locus_tag="VFO30_08425"
     CDS             complement(3234..4028)
                     /locus_tag="VFO30_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HET9419352.1"
                     /translation="MLRAGYSSRAVLEELATRHFADVLDPGKEAMLTRAGAASTLILA
                     LKSGTYTASAQETTAAKEKLAADAARRAQQAEEARKFDTLYQDQLARQRASIRPAAVT
                     GNAILPLVKGDLVSWHNGSLSRFDDEPLEKKKLIALYFSAHWCAPCRKFTPQLVDYYN
                     RVAPQHPEFEIIFVSLDKSQFAFESYIREANMPWPAIDYQKLAGKDAVRKYAGDSIPG
                     LVLLDATGKVISDSSAGKEYLGPAKVLADLDAIFAGRAPANATASR"
     gene            4227..4700
                     /locus_tag="VFO30_08430"
     CDS             4227..4700
                     /locus_tag="VFO30_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419353.1"
                     /translation="MQRDRAFTFLEIVLAIFILMLLLSLAVPSLHGVVADKRLRTSLD
                     SFNDLVRQAQERSVTEQRAYLIVWGEKRVMLRPEALMKEDEAKPTAEYKLNGSDALAL
                     SLPAALVKKPPGEWVFWPSGTCEPAMVKFQGAAGTWTANYPALTCRAELTNYAAR"
     gene            4687..5172
                     /locus_tag="VFO30_08435"
     CDS             4687..5172
                     /locus_tag="VFO30_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419354.1"
                     /translation="MRHGKQSWSVGVVEFWSIAAVGPLPPHSDTRRRHRASGFALYEV
                     LLGLAIFAIGILALGRATQDCLNASTLSATEDRVRQILANRMAEVQTKPGPPDASQES
                     KIDTGYGIVKLTQKSVPADLKDDQNRVLSGINFVTLTAQWNAAGVTQSRVIEFYVYRS
                     G"
     gene            5153..5782
                     /locus_tag="VFO30_08440"
     CDS             5153..5782
                     /locus_tag="VFO30_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9419355.1"
                     /translation="MSIARVKQARSRAFTLLEITLAVAILATMAVAIYRFVEANLTSL
                     RVSSETTTADARYGGLRDLLTAQWQSLSGTNAVLTGEPFKFSGRPRDEIRWTCSSGPG
                     LLTRYAPGDFTVEMRLQPASSKSNRLDLGFLRKPNDDTGASSADQTWVPLIQNVASME
                     IRYFDPRFNGWVERWSEAGRFPRLVKIVIGRTDATVPWEMIIPLGRTPY"
     gene            5854..6729
                     /locus_tag="VFO30_08445"
     CDS             5854..6729
                     /locus_tag="VFO30_08445"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419356.1"
                     /translation="MLALWALFLLSAMIISWALDIHSQLTLSGNANRTLEAEALACSG
                     AEIATHPAVNPGSPNLQRKIDNRKSFDARITGEGGRLNLNWLLAGEDLNRVDILRRYL
                     DNKGIELNDRDAMMDALLDWVEPDTGLHRLNAPPETDDYHPRHAPLTRLEELKQVKGW
                     ADFTSQPNWDDDFTLNSSGPIDLAWATRAVLLALPGMTPDLVDRFLSVRRGPDGIDGT
                     ADDAPFKGIEDARAALGFSQQQFAQLAPLVTFKDPVLRIVSVGRSSEVSRVVQMVIRR
                     ADNVNVPQLITWKEF"
     gene            6821..7966
                     /locus_tag="VFO30_08450"
     CDS             6821..7966
                     /locus_tag="VFO30_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419357.1"
                     /translation="MLRLHNIAEAVPLLSAEDEVVLGLPVGAVLAQRFCLPTVDASEF
                     PEMVRIQMEKVLPFSADEVTTDFELIEQNGNESVISAVAVRNAQLAEIAGPLLERGFI
                     PRQVTVYAAQRASTYAPRGNALLIYPEGETLVSAVTENGKLSLTRTLDGTAPEQLRTE
                     LPQLALSAELQGISSSFPNVLLDETCYEMRDTIEKIVASAPDIVGLETPPAPVKLNLL
                     PESWRQRRAQLARRGELRKRLLWVGGAYVGSFLLLLVYLIAMRATIGYFNSAIARDAP
                     KTQFVRATEANWKALAPAIDPRYYPVEILLHLFESLPSGDVRITTYNQSARQISVEGE
                     ANSPALAYQFIEKIKKNANFQILQFDMGAPRILPNNHAQFRIEGRPR"
     gene            7963..8523
                     /locus_tag="VFO30_08455"
     CDS             7963..8523
                     /locus_tag="VFO30_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419358.1"
                     /translation="MTLAWYERMSGRERLLAWIVAGTVFLLLNVFLWSWLLGAIRRAH
                     TDLASRRAARAQQTVYARERSLWEKRDQWLRQHQPIMKSAAEASTLLDQVKQIAGKYN
                     ILVENPAIGSGETTPYHQTVFASIETKCPWPALVHFLYDLQQPESFLVFETVNLAVDT
                     GDPTLMHGKFKIARWFAPAQRPKPSS"
     gene            8520..9245
                     /locus_tag="VFO30_08460"
     CDS             8520..9245
                     /locus_tag="VFO30_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419359.1"
                     /translation="MRPARLSPNPPSAGSSFNAGEDNSYLKGTGRSFASQPVGSIGCR
                     HAALCGPILCAVIFVFIAASAVVGEDELPKRVGFERYQLMLKQPLFAVATAAAPMPTA
                     TPNFAKDLYVANAAHLPDGDTVTIASSADPNFKKYLSTKAPVDGYGIASIEWSEKVGA
                     TKVTISKDGQFATLTFNEALLSQRPSTPGVPAPVPNVPMPNVPQPAQPNLPGRTRTPH
                     IRGMIPRYPTPATRPNAVPPSEQ"
     gene            complement(9270..9692)
                     /gene="gspG"
                     /locus_tag="VFO30_08465"
     CDS             complement(9270..9692)
                     /gene="gspG"
                     /locus_tag="VFO30_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET9419360.1"
                     /translation="MTSRTTKAKGGFTLLEIMLVVTIIALLLGAAIYKLGGNVEYSRH
                     VRVAADVQGINTQLKVYESMNGFFPTTEQGLQALVTQPTTDPRPVRWYQLYSQMPKDP
                     WQNNYVYRCPGTKNPNGYDLFSPGPDHLPDTGDDDWGD"
     gene            complement(9734..11026)
                     /locus_tag="VFO30_08470"
     CDS             complement(9734..11026)
                     /locus_tag="VFO30_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419361.1"
                     /translation="MFVRASALAFGIAAASLLYAQDVVLMSAHDDYAPVARRARVEPT
                     VEVPRDGGSPTVELPKDGGTPTVEIALNRGEPTIEIATSKPNPAPSLTTGEPRSTTAM
                     QPAARPAPRHQSMMASAWLDLRQNANVKSPTQSAPNWVKAVTMVPAHGDKGSAANTLF
                     HIELAHPGGSYSNLLFRLFFDDKPDAHPQLITRAQSGAEISHSGDLGSGIDLPNSESV
                     VIPMQEVGAIDVVVPGDGKTVRAAYLDWMKNSKTVHPVNTEHRRVALETFAAMPPLQA
                     PEQDMEKFGTVTATLAAETIQLGARIDDGATFRFGIERQPLIALVTFEVASARIDAPP
                     EVYLNGQNIGPATLMLPELADPAYRGEMEALVSQMHFRYTGWLRAQKTVPASALKAGD
                     NELTIANAAGTAPSAVRATQIQMKYLWDKSDYILRPSR"
     gene            complement(11051..12325)
                     /locus_tag="VFO30_08475"
     CDS             complement(11051..12325)
                     /locus_tag="VFO30_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9419362.1"
                     /translation="MPQFAYRARDARGALVEGVLDCADRSMAIRQIEQKQCIPIRIEP
                     VDAAGKIADREKPSRLRAAAPRPVAATPAQQLKIPHGQVLVFTEQLAHLLQAGMTLDE
                     ALSVLEKRLKQPRLEQMTHTLHQALVDGRSFSQALRDFRRVFPPLYVNLVAAGEASGA
                     LPEILLRLVKHLMQAKILRDRIQQALIYPAALAVAGSALVVVFVTYLVPQLTGFMAQT
                     GGALPLPTRILLGVHHAVVTYWWLGLLIVIGAVVALRAFIRNDEGRIAWDRFRLRIPA
                     YGRVRRHGYYAQFSRTLGTLIENGIPLLRALDLVSEIAGNRYLESKLAEARRAVIDGA
                     TLSAALSAQQLFPHLFTDMMAVGEQTGRFAEALQTIADVYERELDRTMVFISTMLPIA
                     IIAVIAVLVGAVVWSILSAVFEMTHSLQLRPH"
     gene            12430..12597
                     /locus_tag="VFO30_08480"
     CDS             12430..12597
                     /locus_tag="VFO30_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419363.1"
                     /translation="MRSLALGVLLGLALTVQAFAIIRSPFPAKPMPPSRGHVVVIDDD
                     AKLQGTVTAPK"
     gene            12750..13457
                     /locus_tag="VFO30_08485"
     CDS             12750..13457
                     /locus_tag="VFO30_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419364.1"
                     /translation="MKASIVISVLFAAVALADDFKTTTGQEYKNVTVSRIEPDGLVVV
                     ASYGIIKIPFKELPNEVQEKYHYDLAAAEKFAAQTATDQHALYLQTKEAKERRQRELE
                     ATLKAQPTAAQAASTPRAPTPKGFSPLRQIIADEHPATAMTALYGTIRQVLDDGLLVR
                     VHETKLIGPERLPGGAIIFLPGTFVDRFYDGDRIQVIGTFVGTYQYGSVIGAKRTARA
                     MQVREIHKLVEFPPNLR"
     gene            13521..13706
                     /locus_tag="VFO30_08490"
     CDS             13521..13706
                     /locus_tag="VFO30_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419365.1"
                     /translation="MRWIIFWLIINSAIGYLVGKPKDRADVAVLLSILLGAIGWLIAR
                     ASTSREHSEMPILRRAD"
     gene            complement(13857..13928)
                     /locus_tag="VFO30_08495"
     tRNA            complement(13857..13928)
                     /locus_tag="VFO30_08495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13894..13896),aa:Cys,seq:gca)
     gene            14025..>14512
                     /locus_tag="VFO30_08500"
     CDS             14025..>14512
                     /locus_tag="VFO30_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9419366.1"
                     /translation="MTLHSNELFARAQKRIPGGVNSPVRAFRNVGGEPFFVARAAGAK
                     VWDVDGNEYIDYVGSWGPAILGHAPKVVIDAVREAATRGLSFGIPNPLEVELAELICT
                     SMSSIEKVRMVNSGTEATMSCIRLARAFTGRDKIIKFDGCYHGHADALLVKSGSGALT
                     HGR"
BASE COUNT         2985 a         4343 c         4219 g         2965 t
ORIGIN      
        1 gccgcacgcg acgcatcggc gcagatgtcg cagatctcat cggaggtgaa gtagccgcag
       61 cgtttgcacg gctgaatggc gcgacgggtc tcgttgatcg cgcgcgcgat ttgctcgggc
      121 gcgtcagcgc gcgcctgaat catccagagc gcaatacgct cggccgagcg cgggccgaca
      181 cccggcattt gccgcaattg cgaaatgagc tcgcggagcg cggccggata ttccgttttt
      241 ctcaacatgc gcctaaccag aaggcgtttc catcagttgc agcgcgagtg gctccagtcg
      301 ccgcagataa agctgcagcg gcgcatcgtc gtcggcgtcg gggccgcgtg ccttctggaa
      361 ttcgctatag gcttcggccc aacgcttttc gcgatacagc acgacaccat tccagaagca
      421 atcgcgccga gcaagatgct ccggcttggc ttcggccgcg agccaaagcg gctcgtaaat
      481 ctcatgtcgc tcctgcgaat tgacaccgct cagaaagtcg atcggccgcg cgacaatttc
      541 gcggctggtt agctcaaacg tttgcggccc gatcaaaatc ctcgagccgt aaaaacgatt
      601 ggccacgcaa aaacgccgcg ccaactcgac cggttcgccg ctcgtaatca atgccgggcg
      661 ttctccgtcg tgcaagggcg cgatgatgac ggtgccgctg ctgatgccga catgcacatc
      721 aggctcgccc gacctctcgc catcactctg ggcgcgcgtc gtgcgaaacg cttgcaccac
      781 atcgagcgca acgcgcaccg ctttgttgcc gtggtcgtcg ttgcgatctg ggaaaccgaa
      841 gatcgcgacc acgccttccc catccgccgc ctcgacatag ccaccggctt ccagaacaag
      901 gtcggtcgcg cgatgaataa atttctcggt cacctcggca aaggctgccg gtgtcgctcc
      961 atcggccaaa tcgtacttgt tcgcgatgtc acagacgaca atactcacat cacaggtctg
     1021 cggctcatct tcaaatggga tctcgcccgc gattagacgg ccgacttgtt gcttggataa
     1081 acggccgccg aagaaggacc gcgctatgaa agtgcgactt cgcgtggtga tcgccgccca
     1141 ccgctcgcct gcggcaaacg ccagcacaac ggcaagcatc gacggcagcg gctgaaagaa
     1201 cgttcgatac agaacacaca cccacgcgag cccagccaac tcaatgagca gcgcgagcac
     1261 gatccagcct aatcggccgc gccgcggagt ggtaagagtg aaagcagcga ctccgaacgc
     1321 gagcacgccg gtgagcaggt attgccagaa atcagcagct gatttcgttg cgccaagatc
     1381 gcgggaaacg agctgcgcga tcggccgctc aatcggcagc aacacgcgtg tcgcgtgcag
     1441 cacggcgata acgccaccga ccgcgacggc aataatcagt gaggtgagca gcggccgttt
     1501 catcgacaat tacgcgtgtg cgcctgcgtc tgccaccatc gcggcgcgct cgggataata
     1561 tgattgcacg accagttcgc tcaaaaattt gtccggcgca tccaccgccg cgcgatcgag
     1621 cggaaatctt ttttgcccgg tatttttgca gatcaaactc agcccgaact gaaaatcctt
     1681 gaacagatgc gcgcagagat cggccatgct catgcgctcg ctctgtgcac gttcgacaag
     1741 tcgcgcggtc gccggctcgt cgaagacaat ctcgattccg tgttcgcgct ggaacttgtc
     1801 cgcaaacgcg cgcacgccgg cttcgagcgc ttttgcttcg tgtctgttcc cttccgccat
     1861 taatcgctcg agcacgcgct gcggttctcg cacaagatcg acagttacgc gcaactggct
     1921 caccccggac ccggcgaggt aatatttgta atcgcggaat agtttctcga aaacagtgag
     1981 caaaccgcgc gctccggttt tctccttggc ggcggcttcg gcgagcaagc gtaacgcgtc
     2041 gtcttcaaaa ctaatctcga tcccgtaagc ccgaaacgcg cgctcgtatt gtcggagcag
     2101 gctgccctcc gaatacttca tgattttata gagatcgttc gtgtcgaggt cttcgcacac
     2161 cacgcggacc ggcaaccgcc caatgaattc cggctcgaac ccgtattcaa caaagtcctg
     2221 ggtcgtgacg tgctggaaca attggttgtc catcacctgc acgggctcgg cgctgaaccc
     2281 gatctgtccc tggcggacac gccgcgccac ctgctccttg agtttctcaa acgcgccgct
     2341 gaccacgaac aggatatggc gcgtgttgat cgtgtcgcgc tttgctttgc cacgcctttg
     2401 gaactcaaac gccgcctgta attgcccttg gagatcattc atgctccgca ccggcacctc
     2461 ggtttcctcc atcaacttga gcaatgtcgt ctgaacaccg cggccgctca cgtcgcgccc
     2521 gattaagctg cctgccgcag caatcttgtc gacctcgtcg atgtaaatga tgccgaactg
     2581 cgcgatgttc acgtcgccgt cggctttgtg cacgagctcg cgcacgagat cttcgacatc
     2641 gccccccacg taaccggtct cactgaactt ggtcgcgtcg gccttgacga acggcacctt
     2701 gatcaaatcg gcgatgtgct tgatcaagta ggttttcccg acgccggtcg ggccgacgag
     2761 gatgacgttc tgcttggcgt actcggtttg ctcagcgcgt ttcgcatctt ccttctcgag
     2821 ctggcgaagg tatttggcgt gattgtaatg atcgcagacc gcgatcgaga gcaccttttt
     2881 cgcttcgtcc tgcttgatca cgaaccggtc gaggtgcgcc ttgatgtcac gcggcagaaa
     2941 atcgaactcg aacggctgct tgtcgatctt ctccggcttc tcctcgacct gagtttctgc
     3001 gacgtccggc tgcccaaacg tcgcgaccga gacgcgatcg ccgaagttcg atttcatgaa
     3061 ctcggtgatc ttcgccttca gttcttctgg cgacggaaat ggcggtggat tcttctccat
     3121 gtggggacga ccaaactgct cagacggcga aatctaacac cgacgcttcc actgtaaacc
     3181 tgcggaggtc ccgggatacc caggggcgac cgaggtgagt gcggggcgtc aggctagcgg
     3241 ctggcggtag cgttggcagg cgcgcggccg gcgaaaatcg cgtcgagatc ggcgaggact
     3301 ttggcggggc cgagatattc tttgccggcg gagctgtcgg aaatgacttt gccggttgcg
     3361 tcgagcagga cgagacctgg gatgctgtcg ccggcatact tccgaaccgc gtccttgcca
     3421 gctagcttct ggtaatcgat ggccggccac ggcatgttcg cttcgcggat gtagctctca
     3481 aaggcgaact gcgatttatc gagactgacg aaaatgattt cgaactcggg gtgctgcggc
     3541 gcgacgcgat tgtagtaatc gaccaattgc ggggtgaact tgcgacatgg cgcgcaccaa
     3601 tgcgcggaga agtagagcgc gatcaatttc tttttctcga ggggctcgtc atcgaaccga
     3661 ctcaggctgc cattatgcca ggaaacgaga tcacctttta ccagcggtag gatggcattg
     3721 cccgtgaccg ctgccggccg aattgacgcg cgttgtcgcg cgagttgatc ttgatacagc
     3781 gtgtcgaatt tgcgcgcctc ttcggcttgt tgcgcgcgcc gcgccgcatc tgcggccagc
     3841 ttttctttcg cggcggtggt ctcctgcgcg ctcgctgtgt aggtgccgct cttgagcgcc
     3901 aagatcaaag tcgaggccgc gccggcgcgc gtcagcatcg cttccttacc gggatcgagc
     3961 acgtcggcga aatgacgggt ggccaattcc tccagcacgg cacggctcga gtaaccggcg
     4021 cgcaacatca gtccaatctc atttgcggta agaggcgcgg ccgcggcgtg cctgctaaac
     4081 gtgagagaga cgcagaggat gcaaaaccac gctcgccaca ttgattatga ggcagcagaa
     4141 catgcgccaa tcgcgtgatt ggacgctgct cttcgtccgc tttgcgcccg ccttgtgcat
     4201 cgagccgcag cgcgtacttt tgccgggtgc agcgcgaccg ggcatttacg tttctcgaga
     4261 tcgtgctcgc catcttcatt ttgatgttgc tgctgtctct ggcggtgccg agcctgcacg
     4321 gcgtggtcgc agataaacgg ctgcggacgt ctctcgatag cttcaacgac ctagtgcgtc
     4381 aggcgcagga gcgcagcgtc acggagcagc gtgcctattt gatcgtgtgg ggcgagaaaa
     4441 gagtgatgtt gcgcccggaa gcgctgatga aagaggacga ggcaaaaccg acggcggaat
     4501 acaagctgaa tggctcggat gcgctggcgc tgagtttgcc tgctgcgctg gtcaaaaaac
     4561 caccgggcga atgggtattc tggccctcgg ggacatgcga accggcgatg gtgaagttcc
     4621 aaggcgcggc cggaacatgg acggcaaatt accccgccct cacctgccgg gccgagctga
     4681 cgaattatgc ggcacggtaa acagagttgg agtgttggag tggtggagtt ttggagtatt
     4741 gcagccgttg gtccgttgcc tccacactcc gatacgcgaa ggcgccaccg cgcgagcggc
     4801 tttgcgctgt atgaagtgct gttgggcctg gcgatcttcg cgatcggcat tctcgcgctc
     4861 gggcgcgcaa cgcaggattg cctgaacgcc agcacgctta gcgcaaccga ggaccgcgtt
     4921 cgtcagattt tggccaatcg catggccgag gtccaaacca agccgggtcc cccggatgca
     4981 tcgcaagaat ccaaaatcga caccggttac gggattgtga agcttacgca gaaaagcgtc
     5041 ccggcagatt tgaaggacga tcaaaaccgg gtgttgagcg ggattaattt cgtcacgctc
     5101 accgcacaat ggaatgccgc cggtgtaaca caatcgcgcg tcatcgaatt ttatgtctat
     5161 cgctcgggtt aaacaggcgc gctcgcgcgc tttcacactt ctagagatca cgctcgccgt
     5221 tgcgattctc gctacgatgg cggtggcgat ctatcgattt gtggaagcga acctcacgtc
     5281 acttcgggtc tcatccgaaa cgaccacagc cgatgcgcgc tacggcggtt tgcgcgattt
     5341 gctcacggcg caatggcaga gcctttccgg aacgaatgcc gtactcactg gcgagccgtt
     5401 caaattcagc ggtcggcccc gcgatgaaat cagatggacc tgctcgagcg gacccgggtt
     5461 gctgacgcgc tacgcgccgg gcgatttcac cgtggagatg cgacttcagc cggcgagcag
     5521 caaaagcaac cggctcgacc ttggtttttt gcgcaaaccg aacgacgaca ccggagcgtc
     5581 gagcgctgat cagacgtggg tgccgttaat ccaaaacgta gccagcatgg agattcgcta
     5641 cttcgatccg cggtttaacg gctgggtcga gcggtggagc gaggccggcc gcttcccacg
     5701 tttggtgaaa attgtcatcg gccgaaccga cgccactgtg ccatgggaaa tgatcatccc
     5761 gctcggtcga acgccgtatt agaagatcgt atgtctctat taaatccgag atccgaaatc
     5821 ggaaatccga aatcgaggtc gggtgctgcg ctgatgctgg cattatgggc gctgtttctc
     5881 ttgagcgcga tgatcatttc gtgggcgctc gacatccatt cgcaattgac gctttccgga
     5941 aatgcgaatc gaacgttgga ggcggaagcc ctagcctgtt cgggcgcgga gatcgcgacg
     6001 catcccgccg tcaatcccgg ctcgcccaat cttcagcgca agatcgacaa tcgtaaaagt
     6061 ttcgacgcac ggatcaccgg cgagggcggc cgcttgaatt tgaattggct gctggctggc
     6121 gaagacctga accgggtaga tattttgcgg cgctatttgg acaacaaagg aatcgagctc
     6181 aacgaccgcg atgcgatgat ggatgcgttg ctcgattggg tcgagccaga caccgggtta
     6241 catcggctga acgcgcctcc agagactgac gattatcatc cacgccacgc gccactcacg
     6301 cgtctcgaag agctcaagca ggtgaagggc tgggccgatt tcacatcgca gccgaattgg
     6361 gacgatgatt tcaccctgaa cagctccggc ccgatcgatc tggcctgggc gacacgcgct
     6421 gtgctcctcg cgctccccgg aatgacgccg gacctcgtcg atcgtttttt atccgtccgt
     6481 cgcggccccg atgggatcga cggcacggct gacgacgctc cgttcaaggg cattgaggac
     6541 gcgcgcgcgg cgcttgggtt ttcccagcag caatttgcgc agttggctcc gctggtcacg
     6601 ttcaaagatc ccgtgctgcg aatcgttagt gtggggagat caagtgaggt aagccgggtc
     6661 gtgcagatgg tgatccgccg ggcggacaat gttaatgtcc cacaactgat tacctggaag
     6721 gagttttgac atcgaagctg agtcgagatc cgttttcatc gttccgacag ctcacggctg
     6781 gaatctgttg cgtccggctg gtcccaaccg ccagtggaat gtgctcaggc tccacaatat
     6841 tgctgaagcg gttccgttgc tgagcgcgga agacgaggtc gtactcggac tgccggtcgg
     6901 cgccgtgctg gcgcagcgtt tttgcttgcc gacggtcgat gcgagcgagt ttcccgagat
     6961 ggtgcgcatt cagatggaaa aggtgctgcc gttttcagcc gatgaagtga cgaccgattt
     7021 cgagctgatc gagcaaaacg gcaacgaaag cgtgatttca gccgtggcag tgcgtaacgc
     7081 gcaactggcc gaaatcgccg ggcccttgct cgagcgcggt tttattccgc ggcaggtgac
     7141 agtgtacgcg gcgcaacgcg ccagcacgta cgcaccgcgg ggcaatgcgc ttctgattta
     7201 tccggaaggc gaaacgctgg tatcggctgt aaccgagaac ggcaagctga gtctcacccg
     7261 gacgctggac ggcaccgcgc ctgagcagtt gcgcacggaa ctgccgcagc ttgcgctcag
     7321 cgccgagctc cagggcatca gctcgtcgtt tccgaatgtg ttgctggacg aaacctgtta
     7381 tgagatgcgc gatacgattg agaagatcgt cgccagtgcc ccggatatcg tcggcttgga
     7441 aacaccgcct gcgccggtca aactcaacct cctgccggaa agctggcggc aacgccgcgc
     7501 gcaattggcc cggcgcggcg aattgcggaa acggctgctc tgggtcgggg gcgcatatgt
     7561 cggctccttt ctccttctgc tcgtctatct cattgcgatg cgtgccacga ttggctactt
     7621 caattccgca atcgcgcgcg atgcgccgaa gacgcagttc gttcgcgcga cagaagcgaa
     7681 ctggaaggcg ctcgcgccag cgatcgatcc tcgctattat ccagtcgaga ttctactgca
     7741 cctgttcgag agcttgccat cgggagatgt ccgaatcacg acctacaacc agtcggcccg
     7801 gcaaatctcc gtagaaggcg aggcgaattc ccccgccctg gcctaccagt tcatcgagaa
     7861 aatcaagaaa aacgcgaact tccagatttt gcaattcgat atgggcgcgc cgcgcatttt
     7921 gccaaacaac cacgcgcagt ttcggatcga gggacgacca cgatgacact ggcttggtat
     7981 gaacgaatga gcggccggga acgccttctg gcatggatcg tggctggaac ggtgtttctg
     8041 ctgttgaacg tgtttctgtg gagttggctg ctgggtgcaa tcaggcgcgc ccatactgac
     8101 ctcgcgagtc gccgagccgc ccgtgcgcag cagacagtct acgcgcgcga gcgttcgctg
     8161 tgggaaaaac gcgaccaatg gctacggcag catcagccaa taatgaagag tgcggcggaa
     8221 gcgtccactt tgctcgacca ggtcaaacag atcgctggga agtacaatat tctcgtagaa
     8281 aatccggcga tcggttcagg cgaaacaacc ccctatcacc aaaccgtgtt cgcctcaatc
     8341 gaaacaaaat gtccctggcc ggcactcgtc cattttctct acgacctgca acaaccggag
     8401 tcgttccttg tgttcgaaac cgtgaacctg gccgtcgaca ctggcgatcc gacgctgatg
     8461 catgggaaat tcaaaattgc gcgctggttc gctccggcgc agcgtccgaa gccttcctca
     8521 tgagacccgc acgattgtcg ccgaacccgc cttcggcggg cagctccttc aatgccggcg
     8581 aagataactc atatttaaaa ggaacaggaa ggagcttcgc gagccagcca gttggttcaa
     8641 tcggctgccg ccacgccgcg ctttgcgggc cgatcctgtg cgcggtgatc ttcgtattca
     8701 tcgccgccag cgccgttgtc ggggaagacg aattgccaaa gcgcgtcggc ttcgagcgtt
     8761 accagctaat gctaaaacag ccgctgtttg cggttgctac ggcagccgca ccgatgccga
     8821 cggccactcc gaattttgcg aaggaccttt atgtcgccaa cgctgcgcat ttgccggatg
     8881 gcgacacggt gacgatcgct tccagtgccg atccgaattt caaaaaatac ttgtcgacca
     8941 aagcgccggt tgacggctac ggcatcgcga gcattgaatg gtcggagaaa gttggtgcga
     9001 ctaaagtgac gatcagcaaa gacgggcaat ttgccacact cactttcaat gaagcattgc
     9061 tctcgcaacg accgagcaca ccgggtgtgc ctgcgccagt tccgaatgtg ccgatgccga
     9121 acgtccccca acctgcacag ccgaatctgc ctggccgcac ccgcacgcct cacatccgcg
     9181 gtatgatacc gcggtatccc actcccgcta cacggcccaa cgctgtgccg ccctcggaac
     9241 agtaattgtc gatctttagc tcggcgcgct taatcgcccc aatcgtcgtc gcccgtgtcg
     9301 gggagatggt ctggtccggg ggaaaacagg tcgtagccgt ttggattttt cgttcccgga
     9361 cagcgataaa cataattgtt ttgccacggg tctttcggca tttgcgaata gagctggtac
     9421 caccgcaccg gccgcggatc ggtcgtcggc tgagtgacca gcgcctgcaa accttgctcc
     9481 gtcgtgggga agaacccgtt catgctttcg tagaccttca gctgcgtatt gatcccctga
     9541 acgtccgcgg ccacgcggac gtgtcgcgaa tactccacgt tgccgcccag cttgtagatc
     9601 gccgccccga gcaaaagcgc gatgatggtc acgaccagca tgatctcgag cagggtgaaa
     9661 ccccctttcg ccttggttgt tctggacgtc atgggtcgaa gctcagaccg cgcagccgcg
     9721 ttttgcgttc aatttatcgg ctcggccgca ggatgtaatc agatttatcc caaaggtatt
     9781 tcatttggat ctgcgtcgcg cgaaccgccg atggcgcggt tcccgccgcg ttcgcgatcg
     9841 tcagttcatt gtcgccagct tttaaagccg acgccggcac ggttttctgc gcgcgcagcc
     9901 agccggtata acgaaaatgc atttggctga cgagcgcttc catttcaccg cgatatgccg
     9961 gatcggccaa ctccggcagc atcaatgtcg ccggtccgat gttctggccg ttcaagtaca
    10021 cctcgggagg cgcatcaatg cgcgcactcg ccacctcgaa tgtgaccaag gcgatgagtg
    10081 gctgccgttc gatgccgaac cgaaatgtgg cgccatcgtc aatccgcgcg ccgagttgga
    10141 tcgtttccgc cgccagagtc gctgtcaccg tgccgaattt ctccatgtcc tgttctggcg
    10201 cttgcaacgg cggcatggcc gcaaatgttt ccaaagctac acgtcgatgc tccgtgttaa
    10261 caggatgcac agttttgctg ttcttcatcc agtcgagata cgcggcgcgc acagttttgc
    10321 cgtcgccggg gacgacaacg tcgatcgcgc caacttcctg catcggaatg acgactgact
    10381 cggaattggg cagatcaatt ccggatccca gatcgccgga atgcgaaatt tccgcgccgg
    10441 actgtgctcg agtgattaac tgcggatggg cgtccggttt gtcgtcaaag aacagccgaa
    10501 acaacaaatt cgaatagctt ccgccaggat gcgccagctc gatgtgaaag agcgtgttcg
    10561 ccgcgctgcc cttgtcgcca tgcgccggca ccatcgtcac cgccttgacc caattgggcg
    10621 cgctttgcgt cggggacttg acgtttgcat tctggcgcaa atccagccat gcgcttgcca
    10681 tcatcgattg gtgtcgcggt gcaggtcgag cagctggctg catcgcagtt gtcgatcttg
    10741 gttcgccggt cgttaggcta ggcgctggat ttggcttcga ggttgctatt tcgatcgtcg
    10801 gctcgccacg gttcagcgca atttcgaccg tcggagtacc gccatctttc ggcaattcca
    10861 cggtcggact gccaccgtct ctcggaactt ccaccgtcgg ctcgacccgg gcgcgtcttg
    10921 ccaccggtgc gtagtcgtcg tgcgcggaca ttagcacgac gtcctgcgcg tagagaagag
    10981 atgccgccgc gataccaaat gcgagcgcgg aggcgcggac aaacatctgg gctgtgagaa
    11041 tatcgtagtt ttagtgtggc cgcaactgca agctgtgcgt catttcgaac acggccgaga
    11101 gaatgctcca gaccaccgcg ccgacgagca ccgcgatcac ggcgatgatc gcaatcggca
    11161 gcatcgtgga aatgaacacc atcgtccggt ctagctcgcg ctcataaacg tcggcgatcg
    11221 tctgcagcgc ttcggcaaaa cgccccgtct gctcgcccac cgccatcatg tcggtaaaca
    11281 aatgggggaa aagctgctgt gcgctcaacg ctgccgacaa cgtagcgccg tcgatcacgg
    11341 cgcgccgcgc ctcagcgagt tttgattcca aataccggtt gccggcgatc tcactcacca
    11401 agtcgagcgc gcgcaacagc ggaatgccgt tctcgatcaa cgtgccgagc gttcgcgaaa
    11461 actgggcata atagccgtgg cgtcgcaccc gtccgtaggc cgggatgcgc aaccgaaatc
    11521 ggtcccatgc aatgcgtccc tcgtcattgc gaataaaagc acgcaacgca acgaccgcgc
    11581 cgatgacgat caataaaccg agccaccaat atgtcacgac cgcatgatgg acgccgagca
    11641 aaatccgtgt cggcagtggc aacgcgccgc cagtttgcgc catgaaaccg gtcaattgcg
    11701 gcacgagata agtcacgaac accactacga gcgcgctgcc cgcaacggcg agcgccgctg
    11761 ggtaaatcag cgcttgctga atgcggtcgc gtagaatttt tgcctgcatc agatgtttaa
    11821 ccaggcgcag caagatctcc ggcagcgccc cgctggcttc accggccgcg accaagttca
    11881 cgtaaagcgg cggaaacact cgtcgaaaat cccgcagcgc ttgcgaaaaa ctgcggccat
    11941 caaccagcgc ctggtgcaac gtgtgcgtca tctgctcaag tcggggctgt ttcaatcgct
    12001 tttccagaac cgacaacgct tcatcgagtg tcatgccggc ctggagtaaa tgcgcgagct
    12061 gttcggtaaa cacgagcacc tggccgtgtg gaattttcag ttgctgtgct ggtgtcgccg
    12121 ctacgggccg cggcgcggca gcgcgcagac ggctcggctt ttcacggtcg gcgattttcc
    12181 ccgccgcatc gaccggctcg atgcggatcg gaatacactg tttttgctcg atctgacgga
    12241 tcgccatgct gcgatcggcg caatccaaaa cgccctcgac caaagcgcca cgcgcgtcac
    12301 gggctcgata agcaaactgc ggcatcgccg gagagattac gtcgaaatct caagcgcgaa
    12361 agtgatgcgg gcagatcatg gcaaattcct gatgctaatt ggcataaatg atgcagacaa
    12421 aacagccaga tgagatcgtt ggccctcggc gtgctgctcg gtcttgcact caccgtgcaa
    12481 gcattcgcaa tcatccgttc gccctttccg gcgaagccga tgccgccatc tcgcggtcac
    12541 gtcgtcgtta tcgatgacga cgcgaagctg cagggcaccg tcacagcgcc caagtagctc
    12601 gcgtactgta gcgcgacgct gtctacccgg caccgcggtg ccagcgcaat gtttctctct
    12661 ttgcgacaag tcattttgat gcagcggttc gcatccgctg gattgacttt gccggtcgaa
    12721 atcggcacaa gaaacgcgga cgcgacgtca tgaaagcatc tattgtgatc tcggtgcttt
    12781 tcgcggcagt cgcgctggcg gacgatttca agacaacgac cggccaggaa tacaagaacg
    12841 ttacggtctc gcgcatcgag cccgacggct tggtggtggt ggcgtcctac ggcattatca
    12901 agattccgtt caaagagctc ccgaacgaag tccaggagaa atatcactac gatctagccg
    12961 cagcggagaa attcgcggct caaactgcga ccgatcagca cgctctttat ctccagacga
    13021 aggaagcgaa ggaaagacgg cagcgagaac tcgaagcgac tctaaaagct cagccgacgg
    13081 cagcgcaagc agcatcgacg ccgcgggcgc cgacaccgaa gggtttctct ccgctgcggc
    13141 aaatcatcgc ggatgagcat cccgcaactg ccatgaccgc cctgtacgga acgatacgcc
    13201 aagttctgga tgacggactt ttggtcaggg ttcacgaaac gaagctgatc ggccccgaac
    13261 gcctgcctgg aggcgcgatc attttcttac ccggaacgtt tgttgaccgt ttttacgacg
    13321 gcgaccgaat ccaagtcatc ggcacatttg tcggcacata tcaatacgga tctgtgatcg
    13381 gtgcaaagcg gactgcgcgc gcgatgcagg ttcgcgagat tcacaagctc gtcgaatttc
    13441 cgccgaatct gagatgaaaa accccggtca gtctgcgcat cgttcacata aacgatcgat
    13501 cgcctgaggc tcttcgcaaa gtgagatgga tcatcttctg gctgatcatc aactcagcca
    13561 ttggctatct tgtcggcaaa cccaaagacc gcgcggacgt tgctgttctt ctctcgattc
    13621 ttctaggagc catcggctgg ctcatcgccc gcgcttctac cagccgcgaa cattcggaaa
    13681 tgcccattct gcgccgagcc gattaaggtc gaagcgcgtg tgtgccgcca ctgcggcaga
    13741 gaagtcgcgc ggacctaagc gcggtcgccc ccgaaagaat aggcgactat cattcgcccg
    13801 tccgaatttg tcagctactt gtcaactttg tcgactcatt ccctcagttc atagacaggc
    13861 gggggcggga atcgaacccg cgaatagcgg ttttgcagac cgcgacctta ccacttggct
    13921 accccgccgc gtcggaacga gagtcgaaat atcagtgtag cggcgcttgt atcaagcgcc
    13981 gaactaaatt aaacaggcca tcgccgcggc ccccgcacca caacgtgacg ctccactcga
    14041 acgaactctt cgcacgcgca cagaaacgca tacccggcgg cgtcaactca cccgtgcgcg
    14101 ccttccgcaa tgttggcggc gagccgttct tcgtggcgcg cgcggcgggc gcaaaagtgt
    14161 gggacgtgga cggcaatgag tacatcgatt acgtcggcag ttggggccca gccatccttg
    14221 gtcatgcgcc caaagtcgtc attgatgcag tccgcgaagc agcgactcgc ggattaagct
    14281 tcggtattcc aaatccgttg gaagttgaat tggcggaact gatctgcacg tcgatgtcgt
    14341 ccattgagaa ggtgcgcatg gtcaacagcg gcaccgaagc caccatgtca tgcatccggc
    14401 tggcccgcgc gtttaccggg cgcgacaaga tcatcaagtt cgacggttgt tatcacgggc
    14461 acgccgacgc cttgcttgtc aaatccggta gcggcgcgct cacgcacggg cg
//
LOCUS       DASTDG010000256         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_861018, whole genome shotgun sequence.
ACCESSION   DASTDG010000256 DASTDG010000000
VERSION     DASTDG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861018"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFO30_08505"
     CDS             <1..783
                     /locus_tag="VFO30_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET9419367.1"
                     /translation="LGFAQGHFADFDAAARVTQIGRDLLRKMIDWLSAHGAQVIEVDT
                     DGIYFMPPDEMEINELQNGLAKELPQGIDIEFDERFEAMFSYKAKNYALLTKNGDVII
                     KGGALKSRGLEKFQRVFLEEMIKLIMEERPAAIVDLRNKFERKIRNREWKIDMLMKTD
                     TLQDSLDKYRAKIAGSARNRAAAYELALASGRSYKPGDQISYYIKSTPKKVPAYEAAK
                     LASDFDPQNRDENIDYYVAKLDELVKKFASVSTNTEQKPLNL"
     gene            1294..1869
                     /locus_tag="VFO30_08510"
     CDS             1294..1869
                     /locus_tag="VFO30_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9419368.1"
                     /translation="MTDPVLDAEQYNKVQLIGGGEERLLDLMRVVEPRLKKLRYAKVT
                     KQPLLYADIGLQALIPASQMGHAFCRMLTLYMEMLVTEAEILLIDEIENGLHHSVYEN
                     VWKGIGALAWSENIQVFVTTHSEECVNAAAKAAKRGDEHGFSRHRLQQFQNEIVVNTS
                     QEIFGETIPAKVGEIMRRSKTTDTEKGDEEK"
     gene            1877..3370
                     /locus_tag="VFO30_08515"
     CDS             1877..3370
                     /locus_tag="VFO30_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9419369.1"
                     /translation="MKHIFAFQLGIILVTSFSVALSPAEENDHADIKAEVAKRHDEAV
                     KRLQDWIGQVSIAAEDRGYPEGAEYMAKLARDAGFQQANVINTDGKPGVFATLDAGAA
                     KTIGLYFMYDVKQFDPAEWTSPPTEARLVDKPPLGKVIIGRGAVNQKGPEAAFLAALH
                     AIRGAGDKMPVNLAMVAEGEEEVGSPHIPQLVRRPEVLAALKKCVGVFMPSAVQDLDG
                     VVTVNLGSKGVVELELVSIGEKWGRGPAKDIHSSLKAMVDSPAWHLVKALDTLVSDDG
                     NEIKIDNYPKPRPLSTDEKEMIAKVSQVRSEAQAKKLFSVQHWINDLPWQQANERLES
                     QPTANIEGLVGGYTGAGGKTVLPHKAVAKMDFRLVPDMKAADALAALKAHLAKRGFGD
                     IEVNMTGGYDPTSTSADSALIKAQVAAYKQFGIDPLFWPRNAGSYPGYVFTGEPLKLA
                     AGHFGLGHGSGAHAPDEYYIIESTNPKIQSFDGTVMSFVEYLYELAK"
     gene            3370..3825
                     /locus_tag="VFO30_08520"
     CDS             3370..3825
                     /locus_tag="VFO30_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9419370.1"
                     /translation="MTDKSAAVVSQDEGRVLRAFGEEVTILLDGERTGGKLTAWTEIT
                     PPGGGPPPHYHSIEDETFHVIEGRVAFLVDGSWREVVTGGSAFMPRGVIHTFKNVGDQ
                     PSRILIMTSPAGFEKFFARCADEFANAGGPDMSRIVQIGAEHGIHFLQQ"
     gene            3929..>4401
                     /locus_tag="VFO30_08525"
     CDS             3929..>4401
                     /locus_tag="VFO30_08525"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET9419371.1"
                     /translation="MTLVVGATGMLGGEICRRLLAAGKSVRALVRSTSDPVKRQNLER
                     LGAELMEGDLKDRSSLDRACRGAAAVLSTPTAIGSKREGDTFQSVDLNGQMQLVDAAR
                     AANAERFIFISVSKGIGESGNPLIEAKRAVEKHLRESGLVYTIVRPSFFMEIWLSS"
BASE COUNT         1080 a         1166 c         1285 g          870 t
ORIGIN      
        1 ctcggtttcg cgcagggcca ctttgcggat ttcgatgccg ccgcgcgggt gacgcagatc
       61 ggccgcgatt tgttacgcaa gatgatcgat tggctgagcg cccacggcgc acaggtcatt
      121 gaggtcgata ccgacggaat ctacttcatg ccgcccgacg agatggagat aaacgaattg
      181 cagaacggtc tcgccaaaga gctgccgcag ggaatcgaca ttgaattcga cgaacggttc
      241 gaagcgatgt tcagctacaa ggcgaagaat tacgcgctgc tgaccaaaaa cggcgacgtg
      301 atcatcaaag gcggcgcact caaatcgcgc ggcctcgaaa aattccagcg cgtttttctg
      361 gaggagatga tcaagcttat catggaagaa aggccagcgg cgatcgtcga tcttcggaac
      421 aaattcgaga gaaagattcg aaatcgggaa tggaagatcg acatgttgat gaagaccgac
      481 acgctccagg attcgctcga caaatatcgg gcgaagatcg ccggctcggc gcgcaaccgc
      541 gccgccgcct acgagctcgc cctggccagc ggccgcagct acaaacccgg cgaccagata
      601 agctattata ttaagagcac gccgaaaaag gtgcccgcct acgaagccgc caagctcgcc
      661 agcgacttcg atccgcaaaa ccgcgacgaa aacatcgatt actacgtcgc caagctagac
      721 gagctcgtga aaaaatttgc ttcggttagc actaacaccg aacaaaaacc tctaaatcta
      781 tagcgatgca acatcgtcct aacgttttgt cgacatatcg tttatgtgat atgtcgcttg
      841 catgctgact ctgttgcgga ttgagtgctt tcggggtctc aaggatcttt cattccagcc
      901 gctatcccga ataaatgtcc tgacaggctt caacaattca gggaaaacga ccgtcctcga
      961 ggcattatac cttctattcg ccacccagga gcagcttgga aattatccga acgtgtttcg
     1021 gagtgcgcaa acggaccagg ctgaacgata cgagcatttc tggcgatggc ttttgccaga
     1081 tggagacctc gataaagaag ctcagcttca tgtgatcacc gataataaaa aaaggctagg
     1141 gatcattgtt aagcgacatc agcaacagcg cggaactttg acagttcaat ttctcgactc
     1201 ctcgcgtggg agtatcagtt ggtccgttgc cggacaccat taggcgcgct tcaagaaaga
     1261 ccttggccgc gaatggagtt cttttccccc cgaatgacgg acccggtcct cgatgccgaa
     1321 caatataata aagtacaatt gatcggcggc ggcgaggaaa ggctcctcga tctgatgcgc
     1381 gtagttgaac cgaggctgaa aaagctgcgc tacgctaagg tcaccaagca gccgcttctt
     1441 tatgccgata tcggactgca agcgcttatc ccagcttcgc aaatgggtca cgctttttgc
     1501 cgaatgctca cgctgtacat ggaaatgctc gtgacagaag cggaaatcct attgatcgat
     1561 gaaatcgaaa atggcctgca tcacagcgtt tatgaaaacg tctggaaagg aataggggca
     1621 cttgcttgga gtgagaatat tcaagtcttc gtgaccactc acagtgaaga atgtgtcaac
     1681 gcagccgcga aggccgcaaa acgtggcgac gagcacggct tttcccgcca ccggcttcag
     1741 caattccaaa acgaaattgt cgtaaacacg tcacaagaaa tctttggaga gaccataccg
     1801 gccaaggtgg gagaaattat gcgacgatca aaaaccacag atactgagaa aggtgacgaa
     1861 gagaaataac gaagtgatga aacatatttt tgcattccag cttggaatta tcttggttac
     1921 atctttcagc gtcgcgcttt cgccggccga agaaaatgac catgccgaca tcaaagcgga
     1981 agtcgcaaaa cgccacgatg aggcggtgaa gcggttgcag gattggatcg ggcaggtgtc
     2041 gatcgcggcg gaggaccgcg gctatcctga aggcgcggag tacatggcca agctcgcgcg
     2101 cgacgcggga tttcagcagg cgaacgtgat caacaccgat ggcaagcccg gcgtgttcgc
     2161 gacgctcgat gcgggcgcgg cgaaaacgat cgggctttat ttcatgtatg acgtgaagca
     2221 gttcgatccg gcggagtgga cctcgccgcc aacggaagcg cgtctggtcg acaaaccgcc
     2281 gctcggaaaa gtgattatcg gccgcggcgc ggtaaaccag aaggggccgg aagcggcgtt
     2341 cctcgcagcg ctgcatgcga ttcgcggcgc gggcgacaag atgcccgtga atctggcgat
     2401 ggtagcggaa ggcgaggaag aagtcggttc gccacacatt ccgcaactcg ttaggcgacc
     2461 agaagtgctg gccgcgctga aaaaatgtgt cggcgtgttc atgccgtcgg cggtgcaaga
     2521 tttggatggc gtcgtgacgg tgaacctcgg ctcgaagggg gtggtcgagc tcgagctggt
     2581 gtcgatcggt gaaaaatggg ggcggggacc ggccaaagac attcactcgt cgctcaaggc
     2641 gatggtcgac agtccggcct ggcatttggt caaggcactc gacacgctcg tgtctgacga
     2701 cggcaacgaa atcaagatcg acaattaccc gaagccgcga ccgttgagca cggatgaaaa
     2761 ggagatgatc gccaaggttt cgcaggtgcg gagcgaagcg caggcgaaaa agctgtttag
     2821 cgtgcagcac tggattaacg atctgccgtg gcaacaggcc aacgaacgac tcgaatcgca
     2881 gccgactgct aacattgaag gactggtcgg cggctatacg ggcgcgggcg gcaagactgt
     2941 gttaccgcac aaagcggtag cgaaaatgga ttttcgtctt gtgccagaca tgaaagcggc
     3001 cgacgcgctc gcggcgttga aagcgcatct ggcgaagcgc ggtttcggcg acatcgaagt
     3061 gaacatgacc ggcggctatg atccaacgag cacctcggcc gactcggcgc tgatcaaagc
     3121 gcaggtcgcg gcctacaaac aattcgggat cgatccgctg ttctggccgc gcaacgctgg
     3181 ctcgtatccc ggttacgtgt tcaccggcga accgctcaag ctcgcagccg gccattttgg
     3241 cctgggacac ggcagcggtg cacacgcacc ggacgaatat tacatcatcg aatccaccaa
     3301 cccgaagatc cagagcttcg acggcaccgt tatgtcgttc gtggaatatc tctacgaatt
     3361 ggcgaagtga tgaccgacaa atcagccgcc gttgttagcc aagatgaagg gcgcgtgctg
     3421 cgcgcgtttg gcgaagaagt caccattctt ctcgatggtg aacggaccgg cggcaaactg
     3481 accgcctgga cagagatcac gccgccgggt ggcgggccgc cgccgcatta ccattcgatc
     3541 gaggacgaaa catttcacgt catcgaaggc cgcgtggcat ttctagtcga cggtagctgg
     3601 cgggaagtcg tgaccggcgg gtcggcgttc atgccgcgcg gtgtgatcca caccttcaag
     3661 aacgtcggcg atcagccgtc gcgcatcttg atcatgacct cgcctgccgg cttcgagaaa
     3721 ttcttcgccc gctgcgcgga tgaatttgca aatgccggtg ggcccgacat gtcgcgcatc
     3781 gttcagattg gcgccgagca cggcattcat tttctccagc agtaagctct cgtttagccg
     3841 ttgccggttt aagtggaatg tgagatcagc cgcaatccgc aaatgttcgt agcaaaggcc
     3901 gctggatacc tatagctatg agcaacgtat gaccctcgtc gttggcgcga ccggaatgtt
     3961 gggtggtgaa atctgccgtc gccttttggc tgccggtaaa tcggttcgcg ccctggtgcg
     4021 atcaacatct gatccggtga agaggcaaaa cttggagagg ctcggagcag agttaatgga
     4081 gggcgacctc aaggaccgct catcgttgga tcgtgcatgt cgcggcgcgg cagctgtgct
     4141 cagcactccg acagccatcg gttcgaaacg cgaaggcgat acatttcaaa gtgtcgacct
     4201 aaacgggcaa atgcagctgg tcgatgccgc gcgcgctgcc aatgccgagc gtttcatctt
     4261 catttccgtg tcaaagggca tcggcgaaag cggcaacccg ctcattgaag cgaaacgcgc
     4321 agtcgagaaa caccttcggg aaagcgggct cgtttacact atcgtgcgac cgagtttctt
     4381 catggaaatc tggctcagtt c
//
LOCUS       DASTDG010000257         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_129364, whole genome shotgun sequence.
ACCESSION   DASTDG010000257 DASTDG010000000
VERSION     DASTDG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129364"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /gene="pyrF"
                     /locus_tag="VFO30_08530"
     CDS             complement(<1..446)
                     /gene="pyrF"
                     /locus_tag="VFO30_08530"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9419372.1"
                     /translation="MTNEKIIVALDVPTKERALNLVEQLRGQISFFKIGLQLYTAEGP
                     EMVKAVRAKGAMVWLDLKLHDIPTTVARAVESASHLGVQMLTIHLSGGAEMIRAAVAA
                     GKNKLLILGVTVLTSADESTLRQIGIPDKIDSQVLRLAKLGVENGI"
     gene            482..1330
                     /gene="ispE"
                     /locus_tag="VFO30_08535"
     CDS             482..1330
                     /gene="ispE"
                     /locus_tag="VFO30_08535"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET9419373.1"
                     /translation="MTEKLRVMQVLAPAKINLSLKILGRRDDGFHEIETLIAPISFYD
                     KIDIARQPRWIDFSCDEPTLPTSDDNLVVRAAKIFFEHTKIKSGVSIKLRKKIPHGAG
                     LGGGSSDAAATLLALNQLFEVKLPPAELAKLGGTIGSDIPFFLFGSAALCTGRGEIVM
                     PVKLTGRLSILLLKPAFAVSSAWAYSRWQSSRQIAGVPYEPLEFAGQAFVNDLEPPVF
                     EKFIFLAQLKMWLLKQPQVGAALMSGSGSTVFAVLRENVDVGLLASRARAEVDSQLWT
                     CACETL"
     gene            complement(1509..2264)
                     /locus_tag="VFO30_08540"
     CDS             complement(1509..2264)
                     /locus_tag="VFO30_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9419374.1"
                     /translation="MNFRLRVWRQKDRQSRGQLVDYDAPDIPANASFLEMLDILNERL
                     LLRGEEPIAFDSDCREGICGTCSLTINGEAHGPDHPGAVCELYMRKFRDRETIVIEPF
                     RVRSFPVIKDLVVDRSALDRIVQAGGFISARAGSAPEANSILVSKTDADVAMEAAACI
                     GCGACAAACPNGSAMLFAAAKVSHLASLPQGQPERARRVVKIVHAMDREGFGNCTNTY
                     ECEAVCPAEISASFIAKLNREYARASLRRSAGE"
     gene            complement(2310..>2457)
                     /locus_tag="VFO30_08545"
     CDS             complement(2310..>2457)
                     /locus_tag="VFO30_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9419375.1"
                     /translation="GSVFEVVSQSFIGRRQGFLNEQNFRAMYSAAEEIGRMLSGLRKS
                     LAST"
BASE COUNT          520 a          722 c          689 g          526 t
ORIGIN      
        1 tcgattccgt tttccactcc aagtttcgcc aaccgcaaga cctgactgtc gatcttatcg
       61 ggtataccga tttgccgcag cgtcgattcg tccgcgctgg tcagcacagt gacgcccagg
      121 atcaacaatt tgttttttcc cgccgccacc gccgcgcgaa tcatttcagc gccgccgctc
      181 aagtgaatcg ttagcatctg caccccgaga tggctcgctg attcgactgc tctcgcaacc
      241 gtggtcggaa tatcgtgcag cttgagatcg agccaaacca ttgcgccttt ggcccgaacc
      301 gccttgacca tttccggccc ctcagcggtg tagagttgca ggccgatctt gaaaaacgaa
      361 atctgtccgc gcagttgctc gactagattg agcgcgcgct cttttgtcgg tacgtcgagc
      421 gcaacgatga tcttctcgtt cgtcatttcg agcagttaga gagtcctcga cttcgctcgg
      481 aatgacagaa aaattgcgcg tcatgcaagt gctcgcgccg gcgaagataa atttgtcgct
      541 caaaatcctc ggccggcgcg atgacggttt tcacgaaatc gagacgctga tcgcgccgat
      601 ttcgttttac gacaagatcg acatcgcgcg ccagccgcgc tggatcgatt tcagttgcga
      661 cgaaccgact ttgccgacca gcgacgataa cctggtcgtc cgcgccgcaa aaattttctt
      721 cgaacacacg aagatcaaaa gcggcgtgtc gatcaagctg cggaagaaaa ttccgcatgg
      781 agccggcctc ggcggcggga gcagcgacgc cgccgcaacg ttgctcgcac ttaaccagct
      841 tttcgaggtg aaattacccc cagctgaact ggcgaaactt gggggaacaa tcgggtcgga
      901 cattcctttc tttctctttg gatcggccgc actctgcaca ggccggggcg aaattgtcat
      961 gccggtgaag ctgacaggga gattatcgat cttgctgttg aagcccgcgt ttgcagtatc
     1021 gagcgcgtgg gcgtattcgc gctggcaaag ctcgcgccaa attgccggcg ttccttacga
     1081 gccgctggag ttcgccggcc aagcattcgt caacgattta gagccgccgg tatttgaaaa
     1141 gtttattttc ctcgcccagc tcaaaatgtg gctgctgaaa cagccgcaag ttggcgcggc
     1201 cttgatgtca ggatccgggt caaccgtctt cgccgtgctg cgcgaaaatg tagatgtcgg
     1261 tcttctcgcc agccgcgcac gcgcagaggt cgattcccag ctgtggacct gcgcgtgcga
     1321 gacgctgtga ttctggggag cgcacgcgtc tcgcgtgctg gtttcggcct cgggccgaaa
     1381 cgaattttgc tttgaattcc agcacatcga acaatcacgc gcagagataa agttcgcgat
     1441 cgcgaggaca cgctcgccaa cacgcgagac gcgcgctacc cagagatgcg cgatcacgcg
     1501 cgcgatgatc actcgcccgc gcttcggcgc aagctcgcgc gcgcgtattc gcggttcagc
     1561 ttggcgatga aactggcgct gatctcagcc gggcaaaccg cttcgcattc gtaagtattc
     1621 gtgcaattgc cgaaaccttc gcggtccatt gcgtgcacga tcttcaccac gcgcctggcg
     1681 cgctccggct gaccctgcgg cagcgaagcc aggtgcgaaa cttttgcggc agcgaaaagc
     1741 atcgccgatc cattcggaca cgccgccgcg catgccccgc atccgatgca cgcggctgct
     1801 tccatggcaa cgtcagcatc ggttttggag accaaaatcg aattcgcctc cggtgccgag
     1861 cccgcgcgcg cggaaatgaa cccgccggct tgcacgatgc gatcaagtgc gctgcgatcg
     1921 acgacgagat ctttgatcac tgggaatgat ctgacccgaa acggttcgat tacaatggtt
     1981 tcgcggtcgc ggaatttgcg catgtaaagc tcgcagacgg cgccgggatg atccggcccg
     2041 tgtgcttcgc cgttgatggt gagcgaacaa gtgccgcaga ttccttcgcg gcaatcggag
     2101 tcgaaggcga ttggctcctc gccacggagg agcaagcgct cattcagaat gtcgagcatc
     2161 tcgagaaacg atgcattcgc cggaatgtcc ggcgcgtcgt aatcgacaag ctggccgcgc
     2221 gattgtcgat ccttctgccg ccagactctc aaccgaaagt tcatagttaa gagttgatag
     2281 ttgatagttc ggcagagacg cgcatgagct tatgttgaag ccaacgattt tcgtaatccg
     2341 ctgagcatgc ggccgatttc ctccgccgcg gagtacatgg cgcggaaatt ttgctcattt
     2401 agaaagccct gacgacgacc aataaaagat tgcgaaacga cttcaaaaac cgaaccc
//
LOCUS       DASTDG010000258         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_830073, whole genome shotgun sequence.
ACCESSION   DASTDG010000258 DASTDG010000000
VERSION     DASTDG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830073"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFO30_08550"
     CDS             <1..865
                     /locus_tag="VFO30_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9419376.1"
                     /translation="DIFINWHAIPDANLKIGQWKAPFGLEQITPDPQLYTIERSLPTG
                     ALTPERQIGAQLWGKPLTNILPDQKDLVTYYTGIFNGNGRNFNNNDNNEFMYVGRVEL
                     LPFKGTVMGQEASLKLGGDYLFSRDETGTNIAPSLNLKVNPDGSLTSFTLPNPDERHG
                     YSFDAWLKIGPFDLIGEYLDERVRPRVGPGVTPGFTEFEANGFYVQGSYFLLPKKLQA
                     VVKWEHLNPGQFGSDGISSIIGGLNYYVHGDNVKLMGEYLHTWSDFREANLQFGDDQF
                     DELLLRLQVVF"
     gene            1286..>3186
                     /locus_tag="VFO30_08555"
     CDS             1286..>3186
                     /locus_tag="VFO30_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9419377.1"
                     /translation="MSTAVIGQPLTRVDGKLKVTGAATYAAEFVRPQLAYGTLIQSTI
                     ANGRVAKMDISAAKSAPGVIGILTRENAPRFKPYPDNLTTKGAPGESRVPLQDDEIHW
                     VGQHLGVLVADSFEQAMHAVSLVRVDYETKPAVVAIDDERALQNSLYPEMFGGREKLQ
                     VKRGDVDAALASAAHKIDIVYSTPVENHNPIETYSTTAEWEAPDRLLIHESTRGIKQL
                     QRIVANALSMPLENVRIVCPFIGGAFGSKGFQWSHTLLAAAAARLVQRPVKLTFTRPQ
                     MFNSAGQRARTVQRFSVGADKDGKLVALRHATTTHSSPVSQYTEPCGGMSRMLYSCPN
                     IEVTHQLVKVNLTSPCPMRAPGEAPGVFALECAMDEMAHKIDMDPLEFRLRNYADIDE
                     NENKPWSSKKLRQCYERGAQKFGWSKRNRKPASMRASDGAQIGFGMATAIYPAAQQKA
                     GATAILNKDGTVVVRSATHEIGTGTYTAMTQFAADALGIPIEKVRFELGDSQFPEAPN
                     NGGSWLTASVAPAVMGACAELKRKVIDLAGSWPNDEAQLTNLFTRAGQQQITAEFMSE
                     PNKDEREKFSFHSFGAVFIEAHVDLFGQVRVKRAAGVYDVGRIINPRLARSQIMSGML
                     FGFGMALMEA"
BASE COUNT          730 a          903 c          917 g          636 t
ORIGIN      
        1 tgacattttc attaactggc acgcgattcc ggacgcgaat ctcaaaatcg gacaatggaa
       61 agcgccattc ggcttggagc agataacgcc cgacccccag ctctacacga tcgagcgcag
      121 tttgcccacg ggcgcgctca ccccggagcg tcaaatcggt gcgcaactct ggggcaaacc
      181 gttaacgaac attctgcccg atcaaaaaga tctcgtcacc tactacactg gcatcttcaa
      241 cggcaacggg cgcaatttta acaacaacga caacaacgaa ttcatgtatg tcggccgggt
      301 tgaacttttg cctttcaagg gcacggtgat gggacaggaa gctagtctca agctcggcgg
      361 cgattatctt ttcagccgcg acgaaaccgg caccaacatc gcgccctcgc tcaatctgaa
      421 agtcaacccc gatggttcac ttacctcttt taccctgccg aaccccgatg aacgacacgg
      481 ctatagtttc gatgcctggc tcaagatcgg cccgttcgat ctgattggcg agtatctcga
      541 tgagagggtg cgcccgcgcg tcggtcccgg cgtcacgccc ggattcaccg agttcgaagc
      601 taacggcttt tacgtgcaag gcagttattt tctgttgccg aaaaaactac aggccgtcgt
      661 gaaatgggag cacctcaacc cgggacaatt cggcagcgac ggcatctctt ccatcatcgg
      721 tggcctgaac tattacgtgc acggcgataa tgtgaaactg atgggtgaat acctgcacac
      781 ctggtcggat tttcgcgaag cgaacctgca attcggcgat gatcagttcg acgaattgct
      841 gctccgcctg caggtagttt tttaatcgat tacataaagt cttcagcagc caagtgcgtt
      901 ttcgcgcgcg atgatctcgg accgcacgag gtaatcatcg cggagaaaaa gttagtcggc
      961 gaaagggtcg atgagaaaac atttcgcgcg gtggcggcgg cggcgctcgc gccggcgaaa
     1021 ggttacaaat acaattcgtt caagattgag ctggccaaac gctccatcgt gcgcgcgctg
     1081 agcacggtcg cggcgatgcc atgatgaccg cgcgttgttt ggattgggta gcgcgcgcgt
     1141 cttgcgtgct ggcgagtgcg tcctcgcgat cgcgaacttt tccgatgcgc ccaactcgct
     1201 cttccagatc gcatttcaag gaaagatcgt ttcggcggga cgccgaaacc agcacgcaag
     1261 acgcgtgcgc tacccgcaaa ctaacatgag tacagcagtc atcggccaac cgctcactcg
     1321 cgttgacggc aaactcaaag tcaccggcgc ggcgacctac gccgccgagt tcgttaggcc
     1381 acaactcgcc tacgggacct tgatacaaag tacaatcgcc aatggccgcg tcgcgaagat
     1441 ggacatttcc gccgcgaaat cggcgcccgg tgtcatcggc attcttaccc gcgaaaatgc
     1501 gccgcgtttc aaaccctatc ccgacaacct gacaacaaag ggcgcgccgg gcgaaagccg
     1561 cgtgccgctg caagacgacg aaattcactg ggtcggacaa catctcggcg tccttgtcgc
     1621 cgactctttt gagcaggcaa tgcacgcggt gtcgcttgtt cgcgttgatt acgaaacgaa
     1681 acctgcggta gttgcgatcg atgacgaacg cgcgctgcag aattctctgt atccggaaat
     1741 gttcggcggt cgcgaaaaat tgcaggtgaa gcgaggggac gtcgacgccg ccctggcgag
     1801 cgccgcgcac aagatcgaca tcgtttattc gacgccggtc gaaaatcata atccgatcga
     1861 gacatactcg acgacggcgg aatgggaagc acccgatcgt ttgctcattc atgagtcgac
     1921 gcgcggcatc aagcaattgc aacgaattgt ggcgaacgcg ttgagcatgc cgctcgagaa
     1981 tgtgcggatc gtgtgtccgt ttatcggtgg cgcgtttggc tcgaaaggat ttcaatggag
     2041 tcacaccttg ctcgcggccg ccgcggccag gctggtgcag cggccggtga agttgacgtt
     2101 cacccgcccg caaatgttta actctgccgg gcaacgcgcc cgcacagtgc agagattctc
     2161 ggttggtgcg gacaaagatg gaaagctcgt cgcgctgcgt catgccacga cgacacattc
     2221 ctcgcccgtt tcccaataca ccgagccctg cggtggcatg tcgcgcatgt tgtattcgtg
     2281 tccgaacatc gaggtcacgc atcagctcgt gaaagtgaac ctgacatcgc cgtgtccgat
     2341 gcgcgcgccc ggcgaagcgc ccggcgtttt cgcgctcgaa tgcgccatgg acgaaatggc
     2401 gcacaagatc gacatggatc cgctcgagtt ccgcctgcgc aattatgccg acatcgacga
     2461 aaacgaaaac aaaccgtggt cgagcaagaa gctgcgccaa tgttacgaac gtggcgccca
     2521 gaaatttggt tggtcgaagc gaaaccggaa accggcttcg atgcgagcat ccgatggcgc
     2581 gcaaatcggc ttcgggatgg cgacggcaat ttatccagcc gctcaacaaa aagctggcgc
     2641 gaccgcgatt ctgaataaag acggcacggt ggtcgtgcgg agcgcgacgc acgagatcgg
     2701 caccggcacc tacaccgcga tgacgcaatt cgccgcggac gcgcttggga ttccgatcga
     2761 aaaagttcgc ttcgagctgg gagactcgca atttcctgaa gcaccaaaca acggcggatc
     2821 gtggctgacg gcaagcgtcg cgccagccgt gatgggcgca tgcgcggaat tgaaaaggaa
     2881 ggtgattgat ctggcgggaa gttggcctaa cgacgaggcg cagcttacga atctcttcac
     2941 gcgagctgga cagcaacaga ttacggccga gttcatgtct gagccaaata aggacgagcg
     3001 agaaaagttt tccttccatt ccttcggcgc ggtttttatc gaggcccatg tcgatctttt
     3061 cggacaggtg cgagtgaagc gcgcggccgg cgtttacgat gtcggacgca tcatcaatcc
     3121 ccgattggcg cgcagtcaga tcatgagcgg aatgttgttt ggtttcggga tggcgttgat
     3181 ggaagc
//
LOCUS       DASTDG010000259         6947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1350151, whole genome shotgun sequence.
ACCESSION   DASTDG010000259 DASTDG010000000
VERSION     DASTDG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350151"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..328
                     /locus_tag="VFO30_08560"
     CDS             140..328
                     /locus_tag="VFO30_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET9419378.1"
                     /translation="MMSRKFVDCREYPSEKNCSLRISGSEEEVLDAAVQHAVSAHGHE
                     NSPELREQIRSLLKDERE"
     gene            347..1213
                     /locus_tag="VFO30_08565"
     CDS             347..1213
                     /locus_tag="VFO30_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9419379.1"
                     /translation="MRGRRLAVVLVAFYFAVILFGGLPDQLILFPSTAPIDAGGATRR
                     TIAFDNGEVEIWTAQSQAARKSERADVYVLRFYGNADRAERWVAAEAEMWNERAVEIW
                     GMNYPGFGGSTGPARLSRIGPAAMAAFDELQQQAKGRPVVIFGASIGTTAALHVAAHR
                     SVAGLILQNPPPLRQMILRQFGWWNLWLLAGPIASQIPHELDSVANAKASRAPAIFLL
                     AEKDEVIAPRYHRLVVDAYAGEKRVITLRGAGHNTAIEGAALADLHNALDWLLPRRHG
                     SAATTSDRPNRQ"
     gene            complement(1182..2180)
                     /locus_tag="VFO30_08570"
     CDS             complement(1182..2180)
                     /locus_tag="VFO30_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9419380.1"
                     /translation="MRHVLLSGLCIGSAVAAIIALLWWFGMRMPGRNVASAAALSADE
                     VRLREELRADVQKLAGEIGERNMMRYPELLAAADFIESSFARAGFEPRRDSYELHGRA
                     CHNIEAEIRGTSPEIVLVGAHYDSVFECPGANDNGSGVAALLALARRFVAKTMPSTLR
                     FVAFVNEEPFYFQTPQMGSYVYASRCKARGDKITAMISLETIGYYSDAPRSQTYPAPG
                     LGMFYPTVGNFIGFVANTHSRALLHRAMSIFRAQKKIPSEGAALPAFIPGVAWSDQWS
                     FWQHGYPGIMITDTAPFRYPHYHMPSDTPDKLDYDRFTLVVSGMQQVIADLAGPTW"
     gene            complement(2184..3311)
                     /locus_tag="VFO30_08575"
     CDS             complement(2184..3311)
                     /locus_tag="VFO30_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9419381.1"
                     /translation="MKKRVPFRGLGAILFKEFIVVWRDPMTLFFMFFPPLVEMIAFGY
                     ALDTDVKHMAMIILNEDRTVESRQFIDRFVNTETFRVVGEVQSIEQMASEIRKGHAYA
                     GLQIPPKFTMQLHNGEAAQVQLLVDGSNSTTALQALNTALGVTLTQSVQALLRETGRR
                     NVPIDIRPQMLYNPTMRSPNFYIPGVIGIVLQIGTLFATAMAVVREREKGTLETLLVS
                     PLSRWGLMLGKLVPYLCIGMTMAVILFLIMRWLFHVPIHGSVTAMMFATLVYVFALLS
                     LGLLVASKAENQMQALQISMTFMLPSVFFSGFVFPRETMPWIFYVVGTLLPATYFISL
                     MRAIILRGAHFFEYWPHLVILVIMSILLFVLCALRFRKKIG"
     gene            complement(3500..4492)
                     /locus_tag="VFO30_08580"
     CDS             complement(3500..4492)
                     /locus_tag="VFO30_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9419382.1"
                     /translation="MALVCFVVMRVMADAMIECEGLTKRFGNFTAVDHVSFSIGKGSI
                     FGFLGPNGSGKSTVIRMLCGILEPSEGTARIDGHDVERDTEAIKDIIGYMSQKFSLYD
                     ELTVDENLIFSGKLYGLRGRALTQRRDELIGVTHLEPYIDRRAQLLSGGWRQRLAMAC
                     SLMHKPTVLFLDEPTAGIDPVARRELWDLLFEFAGEGMTLFVTTHYMDEAERCDHVGY
                     IYMSKLIVCGEPDELKQMPEVSPPGTRRLDVICDHVTTALQAVRQLDGVRSATVFGQS
                     MHLLVDNNVTQDKIDNRLREVGITHADIHEIGPSLEDVFVTLSAKHAVPEQEAA"
     gene            complement(4554..5441)
                     /locus_tag="VFO30_08585"
     CDS             complement(4554..5441)
                     /locus_tag="VFO30_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9419383.1"
                     /translation="MRPLANSTAVSCKIDHQLCAAALITLSILAAGCERRSNAVSGTI
                     EVDEVHVGPRSPGRVEKIFAQEGDHLRAGQPIVQLDAAELKARRDLAAAQIESAIRDA
                     DSQQAQLQFLRDDARRQQDLLKRKVVSPTDAERADSAARAQEKVVEAAKMRVVQAQAQ
                     LADIDAQLAEMQVTAPADSVLEVLSVKVGDVLPANREVATLLLSNHLWVRVYVPEPWL
                     GRIKVGDQVKVRVDSFPNQDFPGTVEQVNRQAEFTPRNVQTVEDRIRQVFGVKIRLPN
                     NDDRLRAGMAADVYFPNVK"
     gene            5472..5876
                     /locus_tag="VFO30_08590"
     CDS             5472..5876
                     /locus_tag="VFO30_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9419384.1"
                     /translation="MKTRYLDHIDLRVKNMARAREFYRKLLPALGFTRERAEQEWTTF
                     YAPGRGRTEAFFGFTEERDHQPNATRISFWANDRAEVDRIATLVREIGGQTLEGPELC
                     VEYTPGYYAFFFEDPDGNKLEICSRENPIIAE"
     gene            5873..6454
                     /locus_tag="VFO30_08595"
     CDS             5873..6454
                     /locus_tag="VFO30_08595"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HET9419385.1"
                     /translation="MSKSTNVSPVTEHARVRAMLEEPRRRRRYLKLIALFKIAKGVLL
                     LILGVSILFLKAHDVWLDAISDWADDEILLQHSKTVHFLLNQLQTVLAGGTLRATGFL
                     ALFYCGVLLTEGIGVYREKRWAEWLMICATSALIPLEVRHFWHQVISHQPFLAATLIL
                     LANCFIVWFLYLVLRRGTTAAPMPAAGELVETR"
     gene            6467..>6947
                     /locus_tag="VFO30_08600"
     CDS             6467..>6947
                     /locus_tag="VFO30_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419386.1"
                     /translation="MLAAWLQASVCNLSLGDAMLTPTRFSYAVLALTIALAGVLHLGA
                     PLLVILFSYFALSQLHSQTKRKWAALLLFVLLVAGIAAAAVYFTREAIRALPDVADTS
                     IPSASAWAQKRQIELPFTDFESLRAAVIRALKEEASYLQSVAHFAGHTASALVFSLIA
                     "
BASE COUNT         1441 a         2059 c         2018 g         1429 t
ORIGIN      
        1 agggagcgac gatggcggcg cggaacacct tcctaatcaa tccagaggga aaaatcgcta
       61 aggtatacac cggcgtaaaa ccggccgagc acagcgagca agtgctaaaa gatctggccg
      121 agctgaagaa gagctgatca tgatgagccg caagttcgtc gactgccgcg aatatccgag
      181 cgagaagaat tgctcgctcc gcatctctgg aagcgaggag gaagtgctcg atgcggctgt
      241 tcaacatgcc gtctctgcgc atggccacga gaattcgccc gagcttcgcg agcagattcg
      301 ctccctcctc aaagacgagc gcgaataatc agccagttgg gcattgatgc gcggacgccg
      361 cctcgcggtg gtgctcgtcg ccttctattt tgccgtgatt cttttcggcg gcctgccaga
      421 tcaattgatt ttgtttccat cgacagcgcc gatcgatgca ggcggagcta cacgccggac
      481 aattgcgttc gataacggcg aggtggaaat ctggacagcg caatctcagg ccgcgcgcaa
      541 gagcgaacgt gccgatgtct acgttctgcg cttttacggg aatgcggacc gggccgagcg
      601 ctgggtcgcg gctgaagctg aaatgtggaa tgagcgggcc gtcgagatct ggggcatgaa
      661 ttatcccggc ttcggtggca gcacgggtcc ggcgcggctg tcgcggatcg gaccagctgc
      721 gatggccgcg tttgatgagt tacagcagca ggcaaaagga cggccggtcg taatttttgg
      781 cgcaagcatc ggtactactg ctgcgcttca cgttgccgcg catcgctccg tcgcaggttt
      841 gatcctgcag aatccaccgc cgcttcgaca gatgatcttg cggcaattcg gctggtggaa
      901 cctgtggttg ctcgccggcc caattgcatc gcaaatccca cacgagctcg acagcgtcgc
      961 aaacgcaaag gcaagtcgcg cgcccgccat tttcctgctg gcagaaaaag atgaggtcat
     1021 tgcgccacgt tatcaccggc tggtcgtcga tgcttacgcg ggcgagaagc gtgtcatcac
     1081 gttgcgcggt gcaggccaca acaccgcaat tgagggcgca gcattggcag atctacacaa
     1141 cgcgctggat tggctattgc cgcgacgaca cggcagcgct gctaccacgt cggaccggcc
     1201 aaatcggcaa taacttgctg catcccactc accacgagcg tgaatcggtc gtaatcgagt
     1261 ttgtcgggcg tgtcgctcgg catgtggtaa tgcggatagc ggaatggtgc cgtgtcggtg
     1321 atcatgatgc ctggatagcc gtgctgccaa aatgaccatt gatccgacca cgccacaccg
     1381 ggaataaacg ccggcagcgc cgcgccttcg cttgggattt ttttctgcgc acgaaagatc
     1441 gacatcgcgc gatgcagcag tgcgcgcgag tgcgtattgg cgacaaaccc gatgaaattg
     1501 ccgacggtcg ggtaaaacat gcctagtccc ggcgccggat aggtttgcga gcgcggcgca
     1561 tcggaataat aaccgatcgt ctcaagactg atcatcgctg tgattttgtc gccgcgcgct
     1621 ttgcaccggc tggcatagac atagcttccc atttgcggcg tttggaaata aaacggctct
     1681 tcgttcacga acgccacgaa ccgcaacgtc gacggcatcg tctttgcaac gaaacgtcgc
     1741 gcgagcgcga gcaaggctgc cactccgctg ccgttgtcgt tcgcgccggg acactcgaaa
     1801 acggaatcgt aatgcgcacc aacgagcacg atctccggtg acgtgccgcg aatctccgct
     1861 tcgatattgt gacaagcgcg gccatggagc tcgtagctat cacggcgcgg ctcaaagccg
     1921 gcgcgtgcaa acgagctctc gatgaagtct gccgccgcga gcaactccgg gtaacgcatc
     1981 atgtttcgtt cgccgatttc gccggcgagc ttctgcacat cggcacgcaa ctcctcgcgc
     2041 aggcgaacct catcggccga caatgcggcc gcacttgcca cgtttcttcc cggcattcgc
     2101 ataccgaacc accacagtaa cgcaattatg gccgcgacgg cgctgccgat gcacaaacca
     2161 ctcagcagaa catgccgcat tcgtcagccg atctttttgc ggaagcgcag ggcgcaaagc
     2221 acaaaaagca agatcgacat gatgacgaga atcacaaggt gcggccagta ttcgaaaaaa
     2281 tgcgcgcctc ggagaatgat ggcgcgcatg agcgaaatga aataggtcgc gggcaggagc
     2341 gtgccaacca cgtagaaaat ccacggcatc gtctcgcgcg ggaagacgaa gccggaaaag
     2401 aagacgctcg gcagcatgaa tgtcatcgag atttgcagcg cctgcatctg gttctcggcc
     2461 ttgctcgcga cgagcaggcc gaggctgagc agcgcgaaga cgtaaacaag cgtggcgaac
     2521 atcatcgccg tcacgctgcc gtggatcggc acgtgaaaga gccagcgcat gatgagaaaa
     2581 agaatcacgg ccatggtcat gccgatgcag agatacggca ccagtttacc aagcatcaaa
     2641 ccccagcggc tgagcgggct cacgagcagc gtctcgagcg tgcccttttc gcgctcacgc
     2701 acgaccgcca tcgctgtcgc aaacagggtt ccgatttgca gcacaatccc gatcacgccc
     2761 gggatgtaga aattcgggct gcgcatggtt gggttgtaaa gcatttgcgg gcggatatcg
     2821 attgggacat tgcgccggcc ggtctcgcgc aacagtgcct gcaccgattg tgtgagcgtc
     2881 acgcccagag cggtgtttaa agcttgtaac gcagtggtcg agttcgagcc gtcgaccagc
     2941 aactgcacct gcgccgcttc gccgttgtgg agttgcatcg tgaatttcgg tggaatttgc
     3001 agtccggcat aggcgtgtcc tttgcggatc tcgctcgcca tttgttcgat gctctgcact
     3061 tcgccgacga cgcgaaacgt ctccgtgttc acaaaccggt cgataaactg gcggctctcg
     3121 accgtgcggt cctcgttcaa gatgatcatc gccatgtgtt tcacgtccgt gtcgagcgcg
     3181 taaccgaagg cgatcatctc gacaagcggt gggaaaaaca tgaaaaacaa tgtcatcggg
     3241 tcgcgccaga cgacgatgaa ttccttgaac agaatcgcgc cgaggccgcg gaaaggaacg
     3301 cgcttcttca tgtcgatgcg gtgaggtgtg ctttgcgatt ttctggggag cacaggctgc
     3361 cagtctgtag ttgccggcaa cttgccggca accatggacc aaaccgttgt ttgcaaatcg
     3421 agcgaagcat ctcggcaagc tgccgagatg aacaggctgg tagcccgtgc tccccagaac
     3481 aaatgcgccg gtccattcgt taagccgcct cctgctcagg cactgcgtgc ttggccgaga
     3541 gcgtgacaaa cacgtcctca agcgacgggc cgatttcgtg gatgtcggca tgagtgatgc
     3601 caacctcgcg caggcgattg tcgatcttgt cctgggtcac gttgttgtcg acgagcaggt
     3661 gcatcgattg accgaatacc gtcgcgctgc gcacgccatc gagctggcgc actgcctgga
     3721 gcgcagtcgt gacgtgatcg catatcacat cgagccgccg cgtccccggt gggctgactt
     3781 ccggcatctg ttttagctca tccggctcac cacacacgat cagcttggac atgtaaatgt
     3841 aaccgacgtg atcgcagcgc tccgcttcgt ccatatagtg agtggtgacg aacaacgtca
     3901 ttccttcgcc cgcgaactcg aagagcagat cccacagctc gcgccgcgca accgggtcga
     3961 tgccggccgt tggttcgtcg agaaacaaaa cggtcggctt gtgcatgagc gagcacgcca
     4021 tcgccaggcg ctgccgccaa ccgccggaga gcaactgtgc acgcctgtcg atgtacggct
     4081 cgagatgagt gaccccgatc agttcgtcgc gccgttgtgt gagtgcgcgc cctcgcaaac
     4141 cgtaaagttt accggaaaaa atgaggttct cgtcgacggt cagttcgtcg tagagcgaaa
     4201 acttttgcga catgtaaccg atgatgtcct tgatcgcttc cgtgtcgcgc tcgacatcgt
     4261 ggccatcgat gcgcgccgtg ccttcgctcg gctcgagaat cccgcataac attcggatga
     4321 cagtcgattt gcctgatccg ttaggcccga gaaatccgaa gatagaacct ttgccgatgg
     4381 aaaacgaaac gtggtcaacg gcggtaaagt tgccgaagcg tttcgtcaac ccttcgcact
     4441 cgatcatcgc atcagccatg acgcgcatca ccacgaagca caccaaggcc acgaaggtgg
     4501 aaaagattta ttcctgtcga ttttgtcttc gtgaacttcg tgttcctcgt ggttcatttt
     4561 acgtttggaa aatacacatc cgcggccatg ccggcacgaa ggcggtcgtc attattcggt
     4621 aaacgaatct tcactccaaa gacctggcgg attctgtctt cgaccgtttg cacgttgcgc
     4681 ggcgtgaatt ccgcctgacg attcacttgc tcgaccgttc cggggaaatc ttgatttgga
     4741 aacgaatcaa cgcgcacttt gacctgatca cccaccttga tgcgcccgag ccacggctcc
     4801 ggcacataga cacgcaccca aagatggttc gacaacagca gcgtggcgac ttcgcggttg
     4861 gccggcagca catcgccgac tttcacactc aacacttcca ggactgaatc ggccggcgcg
     4921 gtcacctgca tctcagccag ctgcgcgtcg atgtcggcga gctgcgcctg cgcctgcaca
     4981 acccgcatct tcgccgcttc gacgactttc tcctgcgcac gcgcggcgct atcggcgcgc
     5041 tccgcatcag tcggcgaaac gactttgcgt ttcagcaaat cctgttggcg cctcgcgtca
     5101 tcacgcagga actggagctg tgcttgctgc gaatccgcat cccgaatcgc ggactcgatt
     5161 tgcgcggcgg caaggtcgcg ccgcgccttt agctcggcgg cgtcgagttg cacgattggc
     5221 tgacccgcgc gcaaatggtc gccttcctgg gcgaagattt tttctactcg cccgggtgaa
     5281 cgcgggccga cgtggacctc gtccacttcg atcgtgccgg aaacagcgtt gctgcgacgt
     5341 tcgcaacccg cggccaagat cgacagcgtg atcaacgcag cagcgcaaag ttgatggtcg
     5401 atcttgcagg acacagccgt tgagtttgct agcggtcgca tcgagagttc gaagttgcaa
     5461 accagtagag aatgaagacg cgatatttgg atcacatcga cctgcgagtg aaaaacatgg
     5521 cccgcgcgcg agagttttac cgcaagcttc tccccgccct cggcttcacc cgtgagcgcg
     5581 ccgagcagga atggactacg ttttacgcgc cgggccgcgg ccgaactgaa gcgtttttcg
     5641 ggttcaccga agagcgagat caccaaccga acgcaactcg catttccttc tgggcgaatg
     5701 accgcgccga ggtcgaccga atcgcgacgt tggtccgcga gatcggcggc caaaccttgg
     5761 aaggcccgga gttgtgcgtg gagtacacgc caggttatta cgcgttcttt ttcgaagacc
     5821 cggacggcaa caagctcgaa atctgctctc gcgagaaccc gatcattgcc gagtgagcaa
     5881 atcgaccaac gtttcgccag tgactgagca cgcacgcgtt cgcgcgatgc tggaggagcc
     5941 gcggcgccgc cggcgctatc tcaagctcat cgcgctgttc aaaatcgcca aaggcgtgct
     6001 ccttctgatc ttgggcgtgt cgattctttt cctgaaagca catgacgtat ggctcgatgc
     6061 catttcagat tgggctgacg acgagattct gctgcagcac agcaagaccg tgcattttct
     6121 cctcaaccaa ttacagaccg tccttgcagg cggaacgctg cgcgcgaccg gtttccttgc
     6181 gctgttttac tgcggcgttt tgctgacgga agggatcggc gtttacaggg aaaagcgctg
     6241 ggccgaatgg ctcatgattt gcgcgaccag cgcgttgatt ccgctggaag tgcgccattt
     6301 ctggcaccaa gtcatttcgc accagccgtt tctcgcggcg acactgatac ttctggccaa
     6361 ctgtttcatc gtctggttcc tctatctcgt cctgagacgc ggcacgaccg cagcgcccat
     6421 gccggccgcg ggcgagctgg tcgagactcg ttaggccaac ttcatcttgc tcgcggcgtg
     6481 gctgcaagcg agcgtatgca acctctcgct cggcgacgca atgctcacac cgactcggtt
     6541 ttcctatgcc gtcctggcgc ttactattgc ccttgccggt gtgttgcatt tgggtgcgcc
     6601 gctgctggtc atcctgttct cctacttcgc actttctcag cttcattctc aaacgaagcg
     6661 caagtgggcc gcattgcttc tattcgtcct tctcgtcgcg ggaattgcgg cggcggcggt
     6721 ctattttacg cgcgaggcta ttcgtgcctt gcccgacgtc gcggacacct cgattccgtc
     6781 cgccagcgcg tgggcgcaga agcgccagat cgagctgccg tttactgact tcgaaagcct
     6841 gcgcgcggcc gtcatccggg cgctgaagga ggaggcgtcg tacttgcaaa gtgtcgcgca
     6901 ctttgccggc cacaccgcga gcgcactggt cttcagtctc atcgcaa
//
LOCUS       DASTDG010000260         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_81734, whole genome shotgun sequence.
ACCESSION   DASTDG010000260 DASTDG010000000
VERSION     DASTDG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81734"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="xseA"
                     /locus_tag="VFO30_08605"
     CDS             complement(<1..728)
                     /gene="xseA"
                     /locus_tag="VFO30_08605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9419387.1"
                     /translation="MTGVTDFFQASSKVFTVGELTRQIRGALETRFGAVWVQGEVSNY
                     KLHPSGHQYFTLKDNRAQIACVIFRNTMPPLRQPLADGAQVQVYGSVSVFEARGQYQL
                     SVQILQPRGLGVLQAKFEALKRKLEAEGLFAPERKRPLPKVPRRIGIITSPSGAAIRD
                     MLNVLRRRAPWLHILISPVRVQGSGAAQEIAVAIRELATPNESFAPVDLIVVTRGGGS
                     MEDLWEFNEEIVARTIADVTVPVVS"
     gene            complement(759..1061)
                     /locus_tag="VFO30_08610"
     CDS             complement(759..1061)
                     /locus_tag="VFO30_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9419388.1"
                     /translation="MRDYDFWVYIVTNRNHSVLYIGLTNSLSRRIWEHRQGSGANFPA
                     AYQCKKLLYYEHYSNVHEAIARESQLKKWSRSKKVTLINRLNPSWLDVGDQVLQEG"
     gene            complement(1285..2283)
                     /gene="ruvB"
                     /locus_tag="VFO30_08615"
     CDS             complement(1285..2283)
                     /gene="ruvB"
                     /locus_tag="VFO30_08615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9419389.1"
                     /translation="MKPPQPDAAFEMSLRPPAFSEFAGQVKVRERLELMVEAAKKRGD
                     VLEHILLSGPSGLGKTTLAYIIAHEMGVNIKNTSGPVIEKAGELAGLLTSLEKGDVLF
                     IDEIHRLQPTIEEYLYPAMEDYRLDIIIDQGPQARSLRLNLPKFTLIGATTRAGMVSA
                     PLRSRFGMSARLDYYNAEEMQKIILRSARLLGVDVDLAGAAEIAARSRGTPRTANNLL
                     RWVRDYAQVKAEGKITGEIADRALAMLEIDRDGFDQWDKRIIETLIHKFSGGPVGLNS
                     LAVAVGEEAGTLEEVNEPYLIMEGYIKRTPQGRVATANAYRKLGLKPPAGAQAELL"
     gene            complement(2369..2773)
                     /locus_tag="VFO30_08620"
     CDS             complement(2369..2773)
                     /locus_tag="VFO30_08620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9419390.1"
                     /translation="MKKMKLQFSSHTANLAFMRRQVRTFLDGYPFSERERMLIVLGVD
                     EACTNIIRHAYKLRDDQPIALSMEGLRKCVRMRLRDYGAQVPAHKMRGREHHIIAPGG
                     FGLRLMKTAFDRIDYILKARGTELVLTKNLEP"
     gene            3035..3778
                     /locus_tag="VFO30_08625"
     CDS             3035..3778
                     /locus_tag="VFO30_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419391.1"
                     /translation="MDSACEPCSIFHMWKSFAAFLLFSASAVAQEAGTAYEALRVVAT
                     QLNRESIKHVISVTGTGGTPQPESWKILIDDPRARGGVREIEVRDGRIESQRTPSRSI
                     VGSSEGATIDTGRLNLDSSGAYAVASHTADKSHTLFSTVSYTLRTNERGEPVWIVSLH
                     KQSGRPVGTIYIGATRGNVTRTEGLFAGAGMEDVETERDVEREGEDVRGIFDRTKSGI
                     SHAFYVAQQEARAMFERVKRSFSDFINGD"
BASE COUNT          783 a         1122 c         1123 g          849 t
ORIGIN      
        1 gagaccactg gcacggtcac gtctgcgatc gtgcgcgcga cgatttcttc attgaactcc
       61 cacaagtctt ccatgctgcc gccgccacgc gtgacgacga tcagatcgac aggggcgaat
      121 gattcgttag gcgtggccag ctcgcggatc gccaccgcaa tctcctgcgc cgcgccgctg
      181 ccctgcacgc gcaccgggct aatcaggatg tgcagccacg gcgcacgccg ccgcaacacg
      241 ttcagcatgt cgcgaatcgc cgctccgctc ggcgaagtta tgatgccgat ccggcgcgga
      301 actttcggga gcggccgttt gcgttcgggc gcgaacaaac cttcggcctc gagtttgcgt
      361 ttcagcgcct caaatttcgc ctgcaaaacg ccgaggccgc gcggctgaag aatctggacg
      421 ctgagctgat actggccgcg cgcttcgaat accgacacgc ttccgtaaac ctgcacctgt
      481 gcgccatcgg cgagcggctg gcggagcggc ggcatcgtgt tccgaaaaat cacgcacgcg
      541 atctgcgcgc ggttgtcctt gagtgtgaaa tactgatgcc cgcttggatg cagcttgtaa
      601 tttgaaactt caccctgcac ccacacggcg ccaaaccgtg tctcaagcgc gccgcgaatt
      661 tggcgcgtca attccccgac cgtgaagacc ttgctggacg cttggaagaa gtccgtcact
      721 cctgtcattc cgagcgaagt cgaggaatct ctcactattc atccttcctg gagcacttgg
      781 tcgccgacat caagccagct tggattcagg cggttaatca atgtgacttt ctttgaccgt
      841 gaccacttct tcaattgtga ttcgcgggca attgcttcat gaacattcga atagtgttcg
      901 tagtagagca gcttcttgca ttgatacgcc gccggaaaat tcgccccact tccctggcga
      961 tgttcccaaa tgcggcgtga aaggctgttt gtcaggccga tgtacaatac cgagtgattg
     1021 cgattagtga cgatatacac ccagaaatcg tagtcacgca tcaggcttgc cggttaacag
     1081 taagagatgt ctcgacttcg ctcgacatga cagtagagag cgcgccacga atttgccgcg
     1141 tcagctcgcc gaccgtgagg atcttcgacg tctgctagaa aaatccgtcg tcagttgcat
     1201 tcactcaaaa ttaatcacga aggacacgaa gacaacgata gttaaaaatt ctttattctt
     1261 cttcgtgatc ttcgtggtga aaactcaaag caattccgcc tgcgcgccgg cgggtggttt
     1321 caagccgagc ttgcgatatg cattggccgt ggcgacgcgg ccttgcggcg tgcgtttgat
     1381 gtagccttcc atgatcaggt aaggctcgtt aacttcctcg agtgttccgg cttcttcgcc
     1441 cacggccaca gcgagtgagt tcaggccaac cgggccgccg ctgaatttgt ggatgagcgt
     1501 ttcgatgatg cgtttgtccc attggtcgaa gccgtcgcgg tcgatctcca gcatggcaag
     1561 cgcgcgatct gctatttcgc cggtaatttt gccttcggct ttgacctgcg cgtagtcgcg
     1621 cacccagcgc aagagattgt ttgcggtgcg cggcgtgccg cggctgcgcg ctgcgatctc
     1681 ggccgcgccg gcgagatcga catctacgcc taacaaacgc gcgctgcgca ggatgatctt
     1741 ttgcatctcc tcggcgttgt agtaatcgag ccgcgcgctc atgccgaagc gcgaccgcaa
     1801 cggcgcgctc accatgcctg cgcgcgtggt cgcaccgatg agcgtgaact tcggcagatt
     1861 gaggcgcagg ctccgcgcct gcggcccctg gtcgatgata atatcgagcc ggtaatcttc
     1921 catcgccggg tagagatatt cttcgatcgt cggttgcagc cggtggattt catcgatgaa
     1981 caggacgtcg cctttttcga gactcgttag gagaccagcg agctcgccgg ctttttcgat
     2041 cacgggtccg ctggtgtttt tgatgttcac gcccatctcg tgggcgatga tgtaggcgag
     2101 agtggtcttg cccaggcccg acggaccgct caacagaata tgttcgagca cgtcgccgcg
     2161 tttctttgcg gcctcgacca ttagctccag acgctcgcgc acttttacct gcccggcgaa
     2221 ctcgctgaat gccggcggcc gcagcgacat ctcaaatgcg gcatcaggct gtggcggttt
     2281 catgctggtt gaaagttaag ttgtaattcg tgaatcgtag aatcggtttt ccacttccag
     2341 ttacgattca ccaatcttga gtcacgattc acggctccaa gttcttcgtc agcacgagct
     2401 cagttccccg cgcttttaga atgtaatcga tgcggtcgaa ggcggttttc atcaggcgca
     2461 gaccgaaacc gcccggggcg atgatgtgat gctcgcgacc gcgcatttta tgggcgggaa
     2521 cttgcgcgcc ataatcgcgc aaccgcatgc gcacgcattt gcgcagtcct tccatcgaca
     2581 atgcgatcgg ctggtcgtct cgcaatttgt aggcgtggcg gatgatgttc gtgcaggctt
     2641 cgtcgacgcc aaggacaatg agcatgcgtt ctctttccga gaacggataa ccatcgagaa
     2701 acgtccgcac ctgccgccgc ataaacgcca gattagccgt gtggctgcta aactggagtt
     2761 tcattttctt catcgccggt cgctaaagcc tgaacacagc tgcgccgctc gttcaagtgt
     2821 cgagtgcgcg gaatgtccgc actgtagaaa acgacaggcc accacatctg cgcgcgcgtc
     2881 atctcagcgt agcgaaggac ctcacaattc gtcgtcttca atcacagaat gtataacgta
     2941 ctcggtgtag cgcagacctg ggctgcgagg ttcctcgctc cgctagaaat gacagccggt
     3001 cttaagcagc gacgcgtcgc gcattgcaaa accaatggac agcgcctgcg agccctgttc
     3061 cattttccac atgtggaagt cattcgccgc gtttcttctg tttagcgcgt cagcagtggc
     3121 gcaggaggcg ggtaccgctt atgaggcgtt gcgcgtcgtc gccacgcaat taaatcgcga
     3181 gtctatcaaa cacgttatct ccgtgactgg caccggcggg acgccgcagc cggagagttg
     3241 gaaaattcta atcgacgatc cgcgcgcccg cggtggtgtg cgcgagattg aggtccggga
     3301 cggacgaatc gagtcacagc ggacgccgag ccgttccatc gtcggctcaa gcgagggcgc
     3361 gacgattgat acggggcggt tgaatctcga ttcgagcggc gcgtacgcgg tggcgagtca
     3421 tactgcggat aaatcgcaca cgctcttctc gaccgtaagc tacacattac gcacgaacga
     3481 gcgcggcgag cctgtctgga ttgtcagctt gcacaagcaa tcagggcggc cggtcggcac
     3541 gatttatatc ggcgcgacgc gcggcaacgt cacgcgaacc gaaggccttt tcgccggcgc
     3601 gggcatggag gatgtggaaa ccgaacggga cgtggagcgc gaaggcgagg atgtgcgcgg
     3661 gatctttgac cggacgaaaa gcggcattag tcacgccttc tacgtcgcgc aacaggaagc
     3721 gcgcgccatg ttcgagcggg tgaagcgttc cttctccgat ttcattaatg gcgattaacg
     3781 cgcgcaacat ggacaagcga tgaccggacg aagccgcgac gaggtctggc cagctctacc
     3841 ctatttggag tggaaagaga cctgcgccac gttgcat
//
LOCUS       DASTDG010000261         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_829264, whole genome shotgun sequence.
ACCESSION   DASTDG010000261 DASTDG010000000
VERSION     DASTDG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829264"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..768)
                     /gene="rdgB"
                     /locus_tag="VFO30_08630"
     CDS             complement(91..768)
                     /gene="rdgB"
                     /locus_tag="VFO30_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET9419392.1"
                     /translation="MEILLATRNRNKLREFRDCFGRDFKVSDLSAFPAVALPEETGHT
                     FAENAALKAVGVSQHRQLQDQHLLVLADDSGLEVDALGGAPGVFSARYAGENATDRDN
                     VDKLLSELSQTRNRAARFRCALALARNSELLKTVEGTVDGMIVDVPRGSCGFGYDPVF
                     VPAGFDQTFAELSSEVKNQISHRADAARQLRRTLQAALDQGFGGCGGGGGGGPGCPPP
                     GPAAVRI"
     gene            810..>2783
                     /gene="leuS"
                     /locus_tag="VFO30_08635"
     CDS             810..>2783
                     /gene="leuS"
                     /locus_tag="VFO30_08635"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9419393.1"
                     /translation="MSSERRKPYPFDQIEPKWQRIWEDRQLFHAPNPGKHGFDPAKPK
                     FYILDMFPYPSGAGLHVGHPEGYTATDIIARYKRMRGFNLLHPMGWDAFGLPAEQYAI
                     KSGQHPATTTRENVAKFKSQLKKIGFSYDWQREINTTDPRYYKWTQWIFLQIYNSWFN
                     PETNKAEPISTYRGDNPNEVRLAYVAEVPVNWCPELGTVLANEEVVDGKSEIGGFPVV
                     RRPMRQWMLRITAYAERLLNELEGLDWPEGIKLLQRNWIGRSEGAEIIFKVAHASGSR
                     SNDDRKRDAYATIRVFTTRPDTLYGATYMVLAPEHSLVDLVVTEEQWPEVRDYRERAA
                     RKSDLERTDLAKEKTGVFTGAFAVNPANNEPIPIWVADYVLPGYGTGAIMGVPAHDER
                     DLEFARNFGLNIVDVVKPPGDEPATGFVGDGIAINSPVINALPTSEAKKKMIAWLEQH
                     GHGRREVNYKLRDWLFSRQRYWGEPFPIVWENGRHRALDESELPVVPPPLDDYKPTGT
                     GEPPLAKATEWIRYSDKATRETNVMPQWAGSCWYYLRFCDPTNDQRFVGTEAERYWMG
                     GDKPGGVDLYVGGTEHAVLHLLYARFWHKVLFDRGCVSKPEPFQRLVNQGIILGEDNQ
                     KMSKSRGNIVNPDDVIAQYGADAFRCYEMFMGPL"
BASE COUNT          609 a          810 c          816 g          548 t
ORIGIN      
        1 ctccatcagc gccgacgggt tggccatgca cggtgaggtg attacgctgc tggatgaagt
       61 gcggcgggtc ggaatcacca agatcggata tcagatccga acggcagctg ggcccggcgg
      121 cgggcagccc ggtccgcctc cacccccgcc accgcagccg ccgaagccct gatctagcgc
      181 cgcttgtagc gtgcggcgca actgccgcgc cgcgtccgcg cgatggctga tttgattttt
      241 cacttcggac gataactcgg caaacgtctg atcgaaacca gccggaacga acaccgggtc
      301 atagccgaac ccgcatgaac cgcgcggaac gtcaacaatc atgccgtcga ccgtgccttc
      361 gacagttttg agaagctcgc tattccgcgc gagcgcgagc gcgcagcgga aacgcgcggc
      421 acgattgcgc gtctgcgaaa gttcgctcag caacttgtcg acgttgtctc ggtcggtcgc
      481 gttttcgccg gcataacgcg ccgagaaaac gccgggcgca ccaccgagcg cgtccacttc
      541 gagaccggag tcgtccgcca atacaagtag atgttgatct tgcagctgtc gatgttgaga
      601 aacgccaacc gctttcagcg ccgcgttctc tgcaaatgtg tggccggttt cctcgggcaa
      661 cgcaacggca ggaaacgcgc ttagatcgct cactttgaaa tcgcggccga agcaatcgcg
      721 aaattcgcgc aacttattcc gattgcgcgt cgccagcagt atttccattt gtttttcctg
      781 cgttcctgct ttcctaagag ataaaatcca tgagcagcga gcggcgcaaa ccgtatccgt
      841 tcgaccagat cgagccgaag tggcagcgca tctgggaaga tcgacagctt tttcacgcgc
      901 cgaatcccgg caaacacggc ttcgatccag ccaagccgaa attttacatc ctcgacatgt
      961 ttccgtatcc cagcggcgcc ggtctgcacg ttggccatcc tgaaggctac accgcgaccg
     1021 acatcatcgc gcgttataaa cggatgcgcg gcttcaatct tttgcatccg atgggttggg
     1081 atgcgtttgg tttgcccgct gagcaatacg cgatcaagag cggccagcac ccggcaacga
     1141 ctacgcgcga gaacgtcgcc aaattcaaat cgcagctgaa gaagatcggg ttttcctacg
     1201 attggcaacg cgagatcaac acaaccgacc cgcgttacta caagtggacg cagtggatat
     1261 ttctgcagat ttacaactcc tggttcaatc cggagacgaa caaagccgaa ccgatctcaa
     1321 cttaccgcgg cgataaccca aacgaagtcc ggctcgcgta tgtcgcggaa gtgccggtga
     1381 actggtgtcc cgagctcggc acggtgctgg ccaatgaaga agtggtggac ggcaagagcg
     1441 aaatcggcgg ctttccagtc gttaggcgac cgatgcgcca atggatgctt cgcattaccg
     1501 cttacgccga gcggttgctc aacgaactgg aaggtctcga ctggccggaa ggcatcaaac
     1561 tactgcagcg aaattggatc ggccgcagcg aaggcgcgga gattattttt aaggtagcgc
     1621 atgcctctgg ctcgcgctcg aacgatgacc gcaagcggga cgcttacgct actattcggg
     1681 tatttacgac aagacccgat acgctttacg gcgcgaccta catggttctc gcgccggaac
     1741 attcacttgt cgatcttgtc gtgacggaag agcagtggcc ggaagtgcgc gattaccgcg
     1801 agcgcgccgc gcgcaaaagc gatctcgagc gcaccgactt ggcgaaggaa aagaccggcg
     1861 tctttaccgg cgcgttcgcc gtcaatccgg cgaataacga acccatccca atctgggtag
     1921 ctgattacgt cctgcctggc tatggcaccg gcgcgatcat gggtgtgccg gcccatgatg
     1981 agcgcgacct ggaattcgcg cgcaattttg gattgaacat tgtcgacgtt gtcaagccgc
     2041 cgggcgacga accagcgact ggatttgttg gcgacggaat cgcgatcaat tcgcccgtca
     2101 tcaacgcgct gccgacgtcg gaagcgaaaa agaaaatgat cgcctggctc gaacaacacg
     2161 gccacggccg gcgcgaagtg aattacaaac tgcgtgattg gctattttcg cgtcagcgtt
     2221 actggggcga accgttccca atcgtttggg agaacggcag gcaccgtgcg ctggacgaat
     2281 cggagctgcc agtcgttccg ccgccgctcg atgattacaa accgaccggc accggcgagc
     2341 cgccgctcgc gaaagcgaca gagtggattc ggtactcgga taaagcgacg cgcgaaacga
     2401 acgtaatgcc gcaatgggct ggttcgtgtt ggtattactt acgtttttgc gatccgacga
     2461 acgatcaacg cttcgttggc acggaagctg agcggtattg gatgggaggc gacaaacccg
     2521 gcggcgttga tctctacgtc ggcgggacgg agcacgcggt gttgcatctg ctttacgctc
     2581 gcttttggca caaggtgctg tttgaccgcg gctgcgtctc gaaacccgag ccgtttcagc
     2641 gactcgtcaa tcagggcatt atcctcggcg aggacaacca gaaaatgtcg aagtcgcgtg
     2701 gcaacatcgt caatccggac gacgtcatcg cgcagtacgg cgcggatgcg tttcgctgtt
     2761 acgaaatgtt catgggcccg ctt
//
LOCUS       DASTDG010000262         9646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_797116, whole genome shotgun sequence.
ACCESSION   DASTDG010000262 DASTDG010000000
VERSION     DASTDG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797116"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..476
                     /gene="rplP"
                     /locus_tag="VFO30_08640"
     CDS             51..476
                     /gene="rplP"
                     /locus_tag="VFO30_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9419394.1"
                     /translation="MPLMPKRVKYRKSQRGSRKGTASRNLRIDFGEFGLQTLERAWIT
                     NTQIEAARVALTRNMKRKGKLWIRIFPDKSVTARPPETRMGKGKGQPEYWVATVRPGN
                     ILFELDGVPESVARESLRLAADKLPVRTKFLTRHHIAAA"
     gene            617..862
                     /gene="rpmC"
                     /locus_tag="VFO30_08645"
     CDS             617..862
                     /gene="rpmC"
                     /locus_tag="VFO30_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9419395.1"
                     /translation="MKIKEIIEMSTDELLTKKRDLRQESLHLRLQQQSGQLEQPSRLR
                     LLRRDVARIETVLTQRTKENAQRPTPNSERRISEKKK"
     gene            866..1165
                     /gene="rpsQ"
                     /locus_tag="VFO30_08650"
     CDS             866..1165
                     /gene="rpsQ"
                     /locus_tag="VFO30_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9419396.1"
                     /translation="MAEQPATTAQPAQVPVRVARAPRKTRVGEVVSNRMNKTIIVQTV
                     TRVPHPRFGKIVKQMNKFYAHDEENKAKAGDTVRIMETRPLSKLKRWRLVEVLQK"
     gene            1178..1543
                     /gene="rplN"
                     /locus_tag="VFO30_08655"
     CDS             1178..1543
                     /gene="rplN"
                     /locus_tag="VFO30_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9419397.1"
                     /translation="MVQIRSRLDVADNTGARMATMIGVIGKQTLYAGIGDVITANVKE
                     ASATGTVKKGEVVRAVLVRTRQPIKRDDGSLLRFDNNAIVIIDKDLNPRGTRIFGPVA
                     RELREKNFMKIVSLAPEVL"
     gene            1671..1961
                     /gene="rplX"
                     /locus_tag="VFO30_08660"
     CDS             1671..1961
                     /gene="rplX"
                     /locus_tag="VFO30_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9419398.1"
                     /translation="MNRLKFHVKKGDNVEVISGNFKGSSGKVLQVLPNKQRVLIEGVR
                     IIKKHLRKSQDNPSGKIAEREGPIHISNVRLIERGEKPAKEKKKKETAKANA"
     gene            1964..2539
                     /gene="rplE"
                     /locus_tag="VFO30_08665"
     CDS             1964..2539
                     /gene="rplE"
                     /locus_tag="VFO30_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9419399.1"
                     /translation="MENEFYRHYKDNVLPALQQRHGYKNVHEVPKVEKVVINTSIGSQ
                     SDVKQALEEAKAELALITGQRPVETRAKKSISNFKLRKDQPIGAKVTLRGERMYEFLE
                     RLIKAALPRIRDFRGVSPRCFDRHGNYTLGVSDQSIFPEVELDKIKRNIGFDVTIVTT
                     ARTDEEAKSLLSEMGVPFSDRARKPAAQSAA"
     gene            2545..2943
                     /gene="rpsH"
                     /locus_tag="VFO30_08670"
     CDS             2545..2943
                     /gene="rpsH"
                     /locus_tag="VFO30_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9419400.1"
                     /translation="MSTVTDPIADLLARVRNGARAQKMDILVPYSKAKAEIVRILKDE
                     GYISDYSLDTSAAHPRIKITNKLVDGASAITGLRRVSRPGLRRYVGTDEIPRVLGGMG
                     VAILSTSRGVLSGREAKKQKIGGELLAYVW"
     gene            2951..3490
                     /gene="rplF"
                     /locus_tag="VFO30_08675"
     CDS             2951..3490
                     /gene="rplF"
                     /locus_tag="VFO30_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9419401.1"
                     /translation="MSRIGNKAVDIPDKVKVNIGNEGVVAVEGPKGKLDWRLPRGISA
                     KIDNNKVTVGRSAETRSVKALHGLSRALINNMVEGVSKGFTKDLEIEGVGFRAAVQGQ
                     NLNLSLGFSHPILFPIPKEIKITVIENTKISISGVDKKTVGQVAADIRRFYPPEPYKG
                     KGVRYAGEQIRRKEGKTVQ"
     gene            3492..3857
                     /gene="rplR"
                     /locus_tag="VFO30_08680"
     CDS             3492..3857
                     /gene="rplR"
                     /locus_tag="VFO30_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9419402.1"
                     /translation="MATFNRKAARDRLHRRIRKKISGTAERPRLAIHFSGKHVYAQVI
                     DDDAARTLVAAATTQKDLLGKNKTAANRATAEIVGRAIAERARAKKIDKVVFDRGGFQ
                     YQGKVKALADAAREGGLQF"
     gene            3861..4421
                     /gene="rpsE"
                     /locus_tag="VFO30_08685"
     CDS             3861..4421
                     /gene="rpsE"
                     /locus_tag="VFO30_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9419403.1"
                     /translation="MAQPTGGRRPDRNPDKTPAAKGDTTEKVVFINRCAKVVKGGRRF
                     SFSALIVAGDHDGMVGYGFGKANEVSEAIRKASEAARKSMEKVSLHENTIPHETIGEY
                     DGARVLLRPASPGTGVIAGGGVRAVAEAAGIKDVLAKSLGSSNHANVVKATIAALRAL
                     RRRDEILKVRGIRADGHATATPGQIP"
     gene            4421..4849
                     /gene="rplO"
                     /locus_tag="VFO30_08690"
                     /pseudo
     CDS             4421..4849
                     /gene="rplO"
                     /locus_tag="VFO30_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            5018..6526
                     /gene="secY"
                     /locus_tag="VFO30_08695"
     CDS             5018..6526
                     /gene="secY"
                     /locus_tag="VFO30_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9419404.1"
                     /translation="MLSAFLNTVKIPELRRRILFTLAVIVIVRLGAAITTPGVNQAVL
                     QEWFRSALTDKSGGSVAALFNLFSGGALENCAIFSLGIMPYISASIMMQLLTAVIPRL
                     GRLAREDGGRQKIMQLTRYATVGLCIFQGYLLAISFQHPESYHTMLPGITDTIRSLGV
                     PLVEDPGWIFRIITIISMTTGTLFLMWLGDQITERGIGNGISLIITVGIVARLPAALA
                     QAWKTFVPSAQTGTSQVSPAILVLMVAFLFLVIAAVIAITQAQRKITVQYAKRVVGRK
                     MYGGQTQYMPLKVNYAGVMPIIFAWALLLFPTTIVGFAFKNSPTAARVAAALSTGWPH
                     YVVLAAMIFFFSYFWVATQFQPAQIADDLKKYGGYIPGVRPGEPTSKFLDYTMTRLTF
                     AGAMFLTLIAVLPSLLSQGLHVPTITAQFFGGTSLLIIVGVMLDTMRQVETHLIQRHY
                     DGFLRKGRIRGRAFDRATYARGEAAEHGTLMWLYVGIAAVVIVGVAAFLVNK"
     gene            6523..7182
                     /locus_tag="VFO30_08700"
     CDS             6523..7182
                     /locus_tag="VFO30_08700"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET9419405.1"
                     /translation="MKRRLVLLGAPGSGKGTQAEMITRQFGIPVTSPGAILRREKDLG
                     TPLGLQTAEIIQHGDLVSDEIIVELIEDWLRLHGAHGFVFDGFPRTLRQAEALLSILQ
                     RLRTALDLAIWLDVSEQTVRDRISGRLQCRSCGFVTSATSAHFADRPVCPYCDGRLER
                     RTDDDATVLQKRLAEFNTKTQPLANFYGKMSILHRIDGNRGREAVFADISRLIEDKVP
                     A"
     gene            7179..7955
                     /gene="map"
                     /locus_tag="VFO30_08705"
     CDS             7179..7955
                     /gene="map"
                     /locus_tag="VFO30_08705"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9419406.1"
                     /translation="MIPIKSAREADKMRQACRTASDILDRVSELVRPGITTKEVDEAA
                     ADFMAEVKVKSAFLGYRLGHRVFPGNICISVNDEIIHGIGSQRRIQYGDIVKLDIGII
                     QDGWVGDNASTVAVGVVDDRTERLLRATETALDRAIAVAYAGGRLGDIGAEIEDEARR
                     NHFTVVREFVGHGVGRKLHEEPQIPNYGRRGSGPKLKPGMTLAIEPMINAGTESVILL
                     DNGWTASTADGMPSAHFEHTVLITKDEPEILTWRAKTQLK"
     gene            7928..8146
                     /gene="infA"
                     /locus_tag="VFO30_08710"
     CDS             7928..8146
                     /gene="infA"
                     /locus_tag="VFO30_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9419407.1"
                     /translation="MARKDAIEVEGTVVELLPNTMFRVELPNGHRLLAHISGKMRLHF
                     IRILPGDKVMLEISPYDLSKGRITYRQK"
     gene            8196..8309
                     /gene="rpmJ"
                     /locus_tag="VFO30_08715"
     CDS             8196..8309
                     /gene="rpmJ"
                     /locus_tag="VFO30_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9419408.1"
                     /translation="MKVRPSVKRLCASCKIVKRKNVVRVICKNPRHKQRQG"
     gene            8412..8807
                     /gene="rpsM"
                     /locus_tag="VFO30_08720"
     CDS             8412..8807
                     /gene="rpsM"
                     /locus_tag="VFO30_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9419409.1"
                     /translation="MPRLLGVEVPADKRVEASLTYIYGIGFPTAKRILEQTNIDPNTR
                     AKDLTPQQLNEIIHVITNNKIRIEGDLRREVQGDLKRLQAINCYRGIRHRRGLPVRGQ
                     RTSTNARTRKGPRKTVGVIRNPEAKAGKV"
     gene            9008..9394
                     /gene="rpsK"
                     /locus_tag="VFO30_08725"
     CDS             9008..9394
                     /gene="rpsK"
                     /locus_tag="VFO30_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9419410.1"
                     /translation="MEPVKIIKAKGSKNVHAGIAHVLATFNNTIVTITDLTGKVIGWS
                     SAGKVGFKGSRKSTAYAAQMVAQDASRQAMGHGLKEVEVRVKGPGAGRESAVRALQAV
                     GLDLTVIKDVTPVPHNGCRPPKQRRV"
     gene            9489..>9646
                     /locus_tag="VFO30_08730"
     CDS             9489..>9646
                     /locus_tag="VFO30_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9419411.1"
                     /translation="MGRYTGPKTKVSRRYGVPLFGSSKSLERKNYPPGMHGPKGSRRK
                     QSDYAIALG"
BASE COUNT         2278 a         2598 c         3000 g         1770 t
ORIGIN      
        1 gcgaagcggc tacagcatca aacgatcaga tgtaacggag cagaattgtt atgccgttga
       61 tgccgaaacg tgtgaagtat cgcaagtccc agcggggaag ccgcaagggg accgcttcgc
      121 gcaacctcag aatcgacttt ggcgagttcg gactgcaaac gctcgaacgt gcgtggatca
      181 cgaacacgca gatagaagcc gcgcgcgtgg cgttgacccg caacatgaaa cgaaaaggca
      241 aactgtggat ccgcattttc ccggataagt cggttacggc gcgtccgccc gaaacacgaa
      301 tgggtaaagg taaaggccaa cccgagtact gggtcgcaac cgtgcgaccc ggcaatattc
      361 tgttcgagct cgatggtgtg ccggagtccg tcgcgcgtga gtcgttacgg cttgccgccg
      421 acaagttgcc ggttcgaaca aaatttttga cacgacacca tattgccgcg gcctaacaag
      481 tgatgaagct gatctgtcat tctggcgcag tggagcgaag cggaacgcag tcgaagaatc
      541 tctcattgta tttagagatg cctcgacttc gctcgacatg acaggaacga gacatcacgc
      601 cgccgcctaa cgagcgatga agatcaaaga aataatcgaa atgagcacgg acgagttgct
      661 gacaaagaaa cgcgatttgc gccaggagag cttgcatctg cgtttgcaac aacagagcgg
      721 gcagctcgag caaccgagcc gtttgcgttt gctgcggcgc gacgtggcgc gaattgagac
      781 cgtgctgaca caacggacga aagaaaacgc ccaacgccca acgcccaaca gcgaacggcg
      841 gatttcagag aagaagaaat aatttatggc cgaacaacca gcaacaacgg cgcagccggc
      901 gcaagtaccg gtgcgtgttg cacgtgcgcc gcggaagacg cgcgtggggg aagtggtctc
      961 taaccggatg aacaagacga ttatcgtgca gacggttacg cgcgtgccgc atccgcgctt
     1021 cggcaaaatc gtgaagcaga tgaacaaatt ttacgcccac gacgaggaga acaaagccaa
     1081 ggccggcgac accgtccgga tcatggagac gcggccgctg agcaagttga agcgatggcg
     1141 gctggtggag gtgctgcaga agtagattgt cgatctaatg gtgcagattc gatccagatt
     1201 ggatgtggcg gacaacaccg gcgcgcgcat ggcgacgatg atcggcgtga tcgggaagca
     1261 gacgctttac gccgggatcg gcgacgtcat caccgccaac gtgaaagagg cgagcgcgac
     1321 gggcacggtg aagaaaggtg aagtggtccg cgcggttctc gtgcgaacgc gccagccgat
     1381 caagcgtgac gacggttcgc tcctgcgctt cgataacaat gcgatcgtca tcatcgacaa
     1441 ggacctgaac ccgcgtggca cacgcatctt cggcccggtc gcgcgcgaat tgcgcgagaa
     1501 aaattttatg aaaatcgtgt cgctcgcgcc ggaggttttg taatgaaaca tgatttagta
     1561 gcacagccct cttggcggtg ggggcgacag gcatcttgcc ttttgtctcg cgcgttcggc
     1621 aggcgagacg cccgccaccc ccgcaggctg gaaggctgtg ccacggcttt atgaaccgac
     1681 tcaagtttca cgtgaagaaa ggtgacaacg tcgaggtgat ctcgggcaac ttcaaaggtt
     1741 cgtcaggcaa agtgctgcag gtgttgccga acaagcagcg ggtgttgatc gagggcgtgc
     1801 ggattatcaa gaagcatctg cgcaaatcgc aggataaccc gagcggcaaa atcgccgagc
     1861 gcgaaggccc gattcacatc tcgaacgtgc gcttgatcga gcgcggcgaa aaaccggcga
     1921 aggaaaagaa gaagaaagaa acagcgaaag cgaacgcctg aaaatggaaa acgaatttta
     1981 ccgccattac aaagacaatg tgctgccggc gctgcaacag cggcacggtt ataaaaacgt
     2041 gcacgaggtg ccgaaggtgg agaaagtggt gatcaacacg tcgatcggct cacaatcgga
     2101 cgtgaagcag gcgttggagg aagccaaggc cgagctggcg ttgatcaccg gtcagcggcc
     2161 ggtcgagacg cgcgcgaaga aaagcatttc gaattttaag ttgcgcaaag accagccgat
     2221 cggcgcgaaa gtgacgttgc gcggcgagcg gatgtatgaa tttctcgagc gcttgatcaa
     2281 agcggcgctg ccccgcatcc gcgactttcg cggagtgtcg ccgcgctgtt tcgaccggca
     2341 cggcaattac acgctcggtg tgtcggacca gtcgattttt cccgaggtcg agctggataa
     2401 aatcaagcgc aacatcggat ttgatgttac aatcgtgacc accgcgcgga cggacgagga
     2461 agccaaatcg ctcctgagcg aaatgggcgt gccgttcagc gaccgcgcgc gcaagcccgc
     2521 ggcgcagagc gcggcttaat tttcatgagc actgttacag atccaatcgc cgaccttttg
     2581 gcgcgtgtgc gcaatggtgc gcgggcgcag aagatggaca tcctcgtgcc gtactcgaag
     2641 gcgaaggcgg agatcgtccg gattttgaag gacgaaggtt acatttccga ttactcgctc
     2701 gacacgagcg cggcgcatcc ccggatcaag atcacaaaca aattagtcga cggcgcgagc
     2761 gccatcactg ggcttaggcg tgtgagccgg ccagggctgc ggcgttacgt cgggacggat
     2821 gaaattccgc gcgtgctcgg cgggatgggc gtggcgattc tctccacctc gcgcggcgtg
     2881 ttgtcgggac gcgaagcgaa gaagcaaaag atcggcggcg agttgctcgc ttacgtttgg
     2941 taaaaatttt atgtcgcgaa ttggaaataa agcggtcgac atccccgata aggtcaaggt
     3001 caacatcggc aatgagggcg ttgtcgcggt ggaaggtccc aaaggcaaac tcgactggcg
     3061 attgccacgg ggcatttcgg cgaagatcga caacaacaaa gtcaccgttg ggcgctcagc
     3121 ggagacgcgc agcgtgaagg cgttgcacgg gctttcgcgc gctctgatca acaacatggt
     3181 cgaaggcgtg agcaaaggct tcaccaaaga cctcgagatt gagggcgttg gcttcagggc
     3241 cgctgtgcag ggtcagaacc tgaacctcag tctcggattt tcgcacccga tcctgtttcc
     3301 gattccgaag gagatcaaaa tcacggtgat cgagaacacc aagatttcga tcagcggcgt
     3361 ggataagaaa accgtcggcc aggtcgccgc ggacattcgt cgcttttatc cacccgagcc
     3421 atataaaggc aaaggtgtgc gttacgccgg cgagcagatt cggcgcaagg agggcaagac
     3481 ggtccagtaa catggcgaca ttcaatcgca aagctgcgcg cgaccgtttg catcgccgga
     3541 ttcggaaaaa gatttccgga acggcggagc gtccgcgcct ggcgattcat ttctctggta
     3601 agcacgttta cgcccaggtc atcgacgacg atgcggcgcg aacgttagtt gctgctgcga
     3661 caacgcagaa ggatttgtta ggcaaaaaca aaaccgcggc gaaccgtgca actgcggaaa
     3721 ttgtcggcag ggcgatagct gagcgcgccc gcgcgaagaa gattgacaag gtggtgttcg
     3781 atcgcggcgg atttcaatac caggggaaag tgaaagcgct cgctgacgcg gcacgcgagg
     3841 gaggattgca gttttaagtt atggcgcaac cgaccggcgg cagaaggcca gatcgtaacc
     3901 cggacaaaac gcccgcggca aaaggcgaca cgaccgagaa ggtcgttttc atcaatcgct
     3961 gcgcaaaagt ggtgaagggc gggcgacgtt tcagtttcag cgcgttgatt gtcgcgggcg
     4021 atcacgatgg aatggtcggt tacggttttg gcaaggcgaa cgaagtatcg gaagcaatcc
     4081 gcaaagcttc ggaagcggcg cggaaatcga tggagaaggt ttcattgcac gagaatacga
     4141 tcccacacga aacgattggc gagtacgacg gcgcgcgcgt cttgctgcgg ccagcatcgc
     4201 ccggcaccgg ggtcatcgct ggtgggggcg tgcgcgcggt cgcggaagcc gcgggaatca
     4261 aagacgtgct ggcgaagtcg ttaggctcga gcaatcacgc caatgtggtg aaggcgacga
     4321 tcgcggcact gcgggcgttg cgccggcgtg atgaaattct gaaggtccgc ggaattcgcg
     4381 cggacggcca cgccacggcg acaccagggc agatcccatg atgcggctac acaatttgcg
     4441 actgcgtcca ggtgcgcgcc atcgcgtcaa gcgcctcggc tgcggcgaaa gctccggcca
     4501 cggcaagacg agtggcaaag ggcacaaagg gcagaaggcg cgctcgggcg gaagtattcg
     4561 gctcggcttc gaaggcggcc agatgccgct catccggcgt ctgccgaagc gcggcttcaa
     4621 caacgcggcc tttcacaagc gctacgcgat cgtgaatctc gatgatcttg gccgattcaa
     4681 agccggctcg accgtcaatg agcaatcgct gcgcgagtcg aagctggtgc gcggtgattt
     4741 tgatgggatc aagattctcg gcgatggcga gttgaagcac ggcctaacga ttgaggccga
     4801 caaggtgagt gcgtctgcgc gcgagaagat cgagaaagct ggcggcactg tgacgctgcg
     4861 cgagacgcca gcgcatacga agacgctacc gaaggaggat gtcgatctta tgccggccga
     4921 agcagttgaa gaacagccaa aagctacatc ggcacctaaa cgtcccgcta aggggaaatc
     4981 ggaagtgaag agaaaagcct cgcggaagaa atagcgaatg ctgtcggcgt ttctaaacac
     5041 ggtcaagata ccggagttgc gccggcggat tttgttcacg ctcgccgtaa tcgtgatcgt
     5101 gcggctcggc gcggcgatca cgacgccggg ggtcaatcaa gctgtgctgc aggagtggtt
     5161 tcgcagcgcg ttgaccgaca aatccggtgg cagcgtcgcg gccctcttca acttgtttag
     5221 cggcggcgcg ctcgagaact gcgcgatctt ctcactcggg atcatgccct acatcagtgc
     5281 gtcaatcatg atgcagttgc tgacggcggt tattccgcga ctcggccggc tcgcccgcga
     5341 ggacggcggc cgccagaaga tcatgcagct gacacgttac gcgaccgtcg gtctctgcat
     5401 ttttcagggg tatttgctcg cgatctcatt tcagcaccct gagtcgtatc acacgatgct
     5461 gccgggaatc accgacacaa ttcgcagcct cggtgttccg ctagtggagg atcccggctg
     5521 gatcttccgg atcatcacca tcatctcaat gacgaccggc acgttgttct tgatgtggct
     5581 gggcgatcag atcactgagc gtggcatcgg caacggcatt tcgctcatca tcactgtcgg
     5641 aattgtggcg cggttacccg cggcgttggc gcaggcttgg aaaacgttcg tgccctcggc
     5701 gcaaaccggc acgagccagg tgagcccggc tattctggtg ttgatggtcg cgtttttgtt
     5761 tttggtaatc gcggccgtga tcgcaatcac acaagcacag cgcaagatca cggtgcagta
     5821 tgctaagcgg gtggtcggcc ggaaaatgta cggcggccaa acacaatata tgccgctgaa
     5881 ggtgaattac gcgggcgtga tgccgatcat tttcgcctgg gcgctgctgt tgttcccgac
     5941 gaccattgtc ggctttgcgt tcaagaacag cccgacggcg gcgcgcgtcg cggcagcgct
     6001 ttcgaccggc tggccgcact acgtcgtgct cgcggcgatg attttcttct tcagttattt
     6061 ctgggtcgcg acgcaatttc agccggcgca gatcgcggac gatctgaaaa agtacggcgg
     6121 ttatatcccg ggtgtgcggc cgggcgaacc gacatcgaag tttctcgatt acacaatgac
     6181 gcggctgacg ttcgccggcg cgatgttcct gacgctgatt gccgtgttgc cgagcctgct
     6241 gagtcaggga ttgcacgtgc cgacgatcac ggcgcaattt ttcggtggca cgagcctgtt
     6301 aatcatagtg ggcgtaatgc tcgacacgat gcggcaggtg gaaacgcacc tgattcaacg
     6361 ccattacgac ggcttcctgc gaaaggggcg gattcgcggc cgtgcatttg atcgcgccac
     6421 ttacgcccgc ggtgaagcag ccgagcacgg cacgctgatg tggctttacg tcggcattgc
     6481 tgcggtagtc atcgttggcg tcgcggcttt tctcgtcaat aagtgaaacg ccgactcgtt
     6541 ttgttaggcg cgccaggctc gggcaaggga acgcaggcgg aaatgattac gcgccaattt
     6601 ggtatccccg tgacctcacc cggagcgatt ctgcggcgcg aaaaggatct cgggacgccg
     6661 cttggtttgc aaacggcgga aatcattcag cacggcgacc tggtttcgga cgagatcatc
     6721 gtcgagctga tcgaggattg gctgcgcctg cacggggcgc atgggttcgt gtttgatgga
     6781 ttcccgcgca ctttgcggca ggccgaagct ctgttgtcga tcttgcagcg gcttcgcacc
     6841 gcgctcgacc tggccatctg gctcgacgta tcggagcaaa ctgtgcgcga ccgcatctcg
     6901 ggtcggctgc aatgtcgtag ttgcggattc gtgacgagtg cgacgagcgc gcacttcgcc
     6961 gatcggccgg tctgccccta ctgcgatgga cgcttggagc gacggacgga cgacgacgcc
     7021 acagtgctgc aaaagcggct cgcggaattc aacaccaaga cgcaaccgct cgccaatttc
     7081 tacggcaaga tgtcgatctt gcaccgtatc gatggcaacc gcggacgcga agccgtgttt
     7141 gcggacattt cgcgcctgat cgaagacaaa gtaccggcat gatcccgatc aaaagcgcac
     7201 gcgaagcgga caagatgcgg caggcctgcc gcacggcgag cgacattctc gatcgggtga
     7261 gcgagctcgt ccgccccggt atcacaacaa aggaagtgga cgaagccgcg gccgatttca
     7321 tggcagaggt aaaagtgaaa agcgcgtttc tcggttaccg gctcggtcac cgcgtttttc
     7381 ctggtaacat ttgcatttcg gtcaacgacg aaatcatcca cgggatcggg agccagcggc
     7441 gcattcaata tggcgacatc gtcaagctcg acatcggcat cattcaagac ggttgggtag
     7501 gggacaatgc cagcaccgta gctgtcggcg tggtggacga tcgcacggaa cgcttattgc
     7561 gcgcgaccga gacggcgctc gaccgcgcga ttgctgtcgc gtacgccgga ggacggctcg
     7621 gggacatcgg cgcggagatc gaagatgaag cgcggcggaa tcatttcacg gtcgtgcgcg
     7681 agttcgtcgg ccatggcgtc ggacgcaaac tgcatgagga gccgcagatt ccaaattacg
     7741 gcaggcgcgg aagcgggccg aaactgaaac cgggtatgac attagcgatc gagccgatga
     7801 tcaacgctgg cacggagtca gtcattctgc tcgacaacgg ctggacggcg tccaccgccg
     7861 atgggatgcc gtccgcgcat ttcgagcaca cggtgttaat cacaaaggac gagccggaaa
     7921 ttttgacatg gcgcgcaaag acgcaattga agtagagggc acggttgtcg agctgttgcc
     7981 caataccatg tttcgcgtcg agctgccaaa cggccaccgc ctcctcgcgc acatctccgg
     8041 caaaatgcgg ctccacttca tccgcatctt gccaggggac aaagttatgc tagagatttc
     8101 cccctacgat ttgtcgaagg ggcggatcac ctaccgccag aaatgacagc gcggacatgg
     8161 ctccgcggtt ttcgttagat cgacaatcac cttttatgaa agtgcgaccg tcagtgaaaa
     8221 gactttgcgc cagttgcaag atcgtgaagc gcaaaaatgt cgtgcgcgtg atctgcaaaa
     8281 acccgcgcca caaacaacgc cagggatgaa ccgcttcaac gctttatccc tttaaccatt
     8341 taacaataag gttgaaccga taaagagttg aagagttgaa gccaaagaaa tttccttaac
     8401 acctcaagaa catgcccaga ttacttggag ttgaagttcc cgcggacaaa cgcgtcgaag
     8461 cgtcgctaac gtacatttat gggatcggct ttccgacggc gaaacgcatt ctcgagcaga
     8521 ccaacattga cccgaacacg cgggcgaagg atctgacacc gcagcagttg aacgagatca
     8581 ttcacgtgat tacgaacaac aaaattcgga tcgagggcga tctccggcgc gaggtgcagg
     8641 gggacttgaa acgactgcag gcgatcaatt gttaccgcgg cattcgtcat cgacggggtc
     8701 tcccggtgcg cggccagcgc acatcgacaa acgcgcggac gcgcaaaggt ccgcgcaaga
     8761 ccgtaggcgt gatccgtaat ccggaggcga aagcaggaaa agtttaacca tggctgaacc
     8821 gaaaacccca gcgaaaacga aagtcgacaa aaagcctgcc gtaaaagcgg ctgaccctga
     8881 aaaaccgatt gctcaggcgc cagcgactga cgagagcaaa acggcaaaga agtctgcgaa
     8941 accgcgcaag gaagaagcgc tcgccgcggc accgccggcg gaaaaccttt cactcgccag
     9001 cgatgacatt gagccggtca aaatcatcaa ggccaaggga agcaaaaatg tccacgccgg
     9061 tatcgcgcat gtgctcgcga cgttcaacaa cacgatcgtc acgattaccg acttgacggg
     9121 taaagtcatt ggctggtcga gcgccggtaa agtgggtttc aaaggatcgc gcaagagcac
     9181 cgcttacgcg gcgcaaatgg tggcgcagga cgcgtcgcga caagcgatgg ggcacgggtt
     9241 gaaggaagtt gaagtgcgcg tcaaggggcc gggcgctggt cgcgagtcgg ctgtgcgcgc
     9301 gttgcaagcg gtagggcttg atctcaccgt catcaaagac gtcacgccgg ttccgcacaa
     9361 tggctgccgc ccaccaaaac agcgccgcgt ctagagaagt taaaggtgtt acaaaggtca
     9421 caatgttaca aaaggcagct tacgagtgag cctctccgat gtaacctcgt aaggatttaa
     9481 cgaaccccat gggcagatac accggaccga aaacaaaagt gagccgccgc tatggcgtgc
     9541 cgcttttcgg atcatcgaaa tcgctcgagc gaaaaaatta tccgccaggc atgcacggac
     9601 cgaagggttc gcggcggaag cagtccgatt acgccatcgc gctcgg
//
LOCUS       DASTDG010000263         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_65093, whole genome shotgun sequence.
ACCESSION   DASTDG010000263 DASTDG010000000
VERSION     DASTDG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65093"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..297)
                     /locus_tag="VFO30_08735"
     CDS             complement(94..297)
                     /locus_tag="VFO30_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419412.1"
                     /translation="MRNMFVLTKREQRVVIAIMMTLIAAVLANHYHQKWRDSAARSAA
                     PVPSMTPAISSPEREGRESDDAP"
     gene            complement(313..1008)
                     /gene="queC"
                     /locus_tag="VFO30_08740"
     CDS             complement(313..1008)
                     /gene="queC"
                     /locus_tag="VFO30_08740"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET9419413.1"
                     /translation="MKHAVVLLSGGIDSATTLAMAISESYETSAMSFEYGQRHRFELE
                     AARRIANSLGAKEHRVIKIDNSIFAGSALTDDVDVPRKRSEEQIGAGIPITYVPARNT
                     IFLAHALAWAEVKGAGHIFIGVNAIDYSGYPDCRPEFIAMFETLANLGTRAGVQGRRF
                     QVHAPLINLSKADIIRKALELNVDLALTHSCYDPAPDSRACGECDSCRLRLKGFREVG
                     VPDPIRYAQPVMR"
     gene            complement(1069..1518)
                     /gene="queF"
                     /locus_tag="VFO30_08745"
     CDS             complement(1069..1518)
                     /gene="queF"
                     /locus_tag="VFO30_08745"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET9419414.1"
                     /translation="MTGRSNTSRLTLLGRSEAKIPSSPAEARLETFLNPARRKYWIHF
                     ETDDFTSLCPVTAQADFARIQIDYVPDRRCVESKSLKFYLASYRNERAFNEAVTNRIL
                     DDFVKACAPREAIVTAQFSARGGIALSVRAEYPDKTTTVKKISRDGR"
     gene            1576..1935
                     /locus_tag="VFO30_08750"
     CDS             1576..1935
                     /locus_tag="VFO30_08750"
                     /inference="COORDINATES: protein motif:HMM:NF042458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6572 domain-containing protein"
                     /protein_id="HET9419415.1"
                     /translation="MPASEDRQDHIGIIDMIAHDAKRDEIVLVMNEPRAWDGTDAQLH
                     QLQERFNAYASFLLDGELAEAHPELVGKRARIEVRCAQMPDTRAIDLLGMIRDQLAFQ
                     DIQVEVVVQDRQASVSI"
     gene            complement(1925..2455)
                     /locus_tag="VFO30_08755"
     CDS             complement(1925..2455)
                     /locus_tag="VFO30_08755"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9419416.1"
                     /translation="MAGGNIDNLRAALRDVPDFPKKGIIFKDITPILSDPALFRQSID
                     LFLERCRGIKIDKIVGIDARGFLFGSTVAYELGVGFIPIRKRGKLPYRTETAKYSLEY
                     GEAEMEMHIDAITRGERVVLVDDLLATGGTSAAAAALIKKVGGELVEAQFLIELEFLH
                     GRERLAPVTVTAFLKY"
     gene            2524..>2961
                     /locus_tag="VFO30_08760"
     CDS             2524..>2961
                     /locus_tag="VFO30_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419417.1"
                     /translation="MRIPGKLFWTSLPLIAGLVVPSLILIAVETIAGRIPFGAALTDV
                     WSQQFADGENVFLLSAIGLIPFALLSIFSFFAARWLSPRRLACVALGGLLGILLYMVP
                     AHATIWYPLCAGGHISSTAAITFLFIPFYCVPALAVGLLVGWIV"
BASE COUNT          576 a          853 c          857 g          675 t
ORIGIN      
        1 ttcaaagtcg ggccgatcgc gccgaaaaaa gaaggcgctt ccccagtctt tgtcgggtat
       61 ggccgcattc ctggcttacc gagtcgataa gaatcacggc gcgtcatcgc tttcgcggcc
      121 ttctcgttct ggtgacgaaa ttgctggcgt catgctcggg accggagcgg ccgatcttgc
      181 agccgaatct cgccactttt ggtggtagtg attcgcgagc accgcggcga taagcgtcat
      241 catgatagca atgacaacgc gctgctcacg tttggtgagc acgaacatgt ttctcatgcg
      301 cctttgccaa agctaccgca tgacaggctg cgcgtagcga atcggatcgg gcacgccaac
      361 ttcgcgaaaa cctttcaggc gtaaccggca ggagtcgcac tcgccgcagg cgcggctgtc
      421 cggcgcggga tcgtagcaac tatgagtgag cgcaagatcg acatttagtt cgagtgcttt
      481 gcggataata tcggccttcg agagattaat cagtggcgcg tgaacttgga accggcggcc
      541 ctgcacgcct gctctcgtgc cgaggttcgc caacgtctcg aacatcgcaa tgaactccgg
      601 ccggcagtcg ggatagccgc tgtagtcgat cgcgttgacg ccgatgaaaa tgtggccagc
      661 gcctttgact tccgcccagg cgagcgcgtg cgcgaggaaa atggtgttcc gtgccggcac
      721 gtaagtgatc ggaatccccg cgccgatttg ctcttcagat cgtttcctcg gaacatcgac
      781 atcatccgtc aacgccgagc cggcaaagat cgaattgtcg atcttaataa ctcgatgctc
      841 tttcgcgcct aacgaattgg cgatgcgccg ggcggcttcg agctcaaacc ggtgccgctg
      901 tccatattcg aacgacattg ccgaggtttc gtagctttcc gaaatcgcca tcgccaacgt
      961 tgttgcggag tcgatcccgc cgctcaggag aacgaccgca tgtttcatgt gttgtcgcaa
     1021 gtttgtcagg cgcgcgccgt ggatcgcaac ttggaaaatt gtgccacttc atcggccatc
     1081 gcggctaatc ttcttcacgg ttgtcgtttt gtccgggtat tcggcgcgga cgctcagcgc
     1141 gatcccgccg cgggctgaga attgcgccgt cacaatcgcc tcgcgtggcg cgcacgcttt
     1201 cacgaaatca tccaggatgc ggttcgtcac cgcttcattg aaggcgcgct catttcggta
     1261 cgaggcgaga tagaatttga gcgacttgct ttcgacgcag cgccggtccg gcacatagtc
     1321 gatctggatg cgagcgaaat cggcttgggc ggtgacggga cacaacgacg tgaaatcgtc
     1381 cgtttcaaag tgaatccaat acttccgtcg cgccgggttg agaaacgtct cgagacgcgc
     1441 ttcggcgggc gagcttggaa tttttgcttc gctgcgaccg agcaaagtca atcggctggt
     1501 gttggaacgt cccgtcatcg cttgtctgct tgaacgttca acgttccgcg ggcaaccttc
     1561 aactcccaac cgctcatgcc tgcgtcggaa gatcgacaag accacatcgg gatcatcgac
     1621 atgatcgcgc acgacgcgaa gagggacgaa atcgtgctcg tgatgaacga accccgtgcg
     1681 tgggacggca cagatgcgca gctgcaccag ttgcaggaac gctttaacgc ctacgcctct
     1741 tttctgctcg acggcgaatt ggccgaggcg catccggagc ttgtcggaaa acgcgctcgg
     1801 atcgaggtgc gctgtgctca gatgccggac acgcgcgcaa tcgatttgtt aggcatgatc
     1861 cgcgaccagc tcgcgtttca ggacatccag gttgaggtgg tggtgcaaga tcgacaagca
     1921 agcgtcagta tttgaggaac gccgtgaccg tgaccggggc gagccgctcg cggccgtgga
     1981 gaaactccag ctcgatgaga aattgcgctt ccacgagttc gccgccgacc tttttgatta
     2041 gagctgccgc ggctgccgaa gttccacctg ttgccagcag atcgtcgacc agcacgacgc
     2101 gctcgccgcg cgtaatcgcg tcgatgtgca tctccatctc tgcttcaccg tactcgagtg
     2161 agtatttcgc cgtctcggtt ctgtaaggca acttgccgcg cttccgaatc ggaatgaacc
     2221 cgacgccgag ttcgtaagcg accgtcgatc caaaaagaaa cccgcgcgcg tcgatgccga
     2281 cgatcttgtc gatttttatc ccgcggcaac gctccagaaa cagatcgatt gactggcgaa
     2341 ataacgccgg atcgctcaag atcggcgtga tgtccttaaa gatgatgccc tttttcggaa
     2401 aatcgggcac atcgcgcagc gcggcgcgta agttgtcgat gttgcccccg gccatcggcg
     2461 cgatgctaac gactcactct gaaacgtcaa tctcaccggc gaagtagctg gcctaactac
     2521 ctgatgcgca taccaggaaa gctcttctgg acatccttgc cgctgatcgc aggattggtg
     2581 gtaccgtcgc ttatcttgat tgcggtggag acgattgccg gccgaatacc gtttggcgct
     2641 gctctgaccg atgtctggtc gcagcaattt gctgacggtg aaaatgtgtt cctgctctcc
     2701 gcgattgggc tcattccatt cgcgttattg tcgatcttct catttttcgc tgcgcggtgg
     2761 ttgtcaccgc ggcgtcttgc gtgcgttgcg cttggtggct tattgggcat tctcctctac
     2821 atggttccag cgcatgcgac aatttggtat ccgctctgcg cgggcggcca catatcctcg
     2881 acagcggcga taaccttcct gtttattccg ttttattgcg tcccagctct ggccgtgggg
     2941 ctgctcgtcg gctggatagt c
//
LOCUS       DASTDG010000264        15709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1345132, whole genome shotgun sequence.
ACCESSION   DASTDG010000264 DASTDG010000000
VERSION     DASTDG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 15709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15709
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345132"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFO30_08765"
     CDS             <1..552
                     /locus_tag="VFO30_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419418.1"
                     /translation="AFKEEFPYLRADSGQLFEFDRDWLYAAINRAADAAGYPSWWLTD
                     HVTESIAFYLRLRNDENVVAFRQLAETVQYVFKAIGYKEIIPYFAPAPPPISLSLLEI
                     AHAAGTGYELAFFDLLEKRLTALIETGVDNLRFSGLQRCVKFVRGVRVWTRGCDVLRE
                     EIVCFIRERLTSTTAERLKCELR"
     gene            556..894
                     /locus_tag="VFO30_08770"
     CDS             556..894
                     /locus_tag="VFO30_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET9419419.1"
                     /translation="MTIFEKIIAREIPAKIIWEDDEAVAFHDVNPQAPVHVLIVPKRV
                     IPRLAVVTEADRATLGKLLLVAGELARKLGIEQTGYRVIINSGPDAGESVPHLHVHLL
                     GKRALAWPPG"
     gene            1047..1748
                     /locus_tag="VFO30_08775"
     CDS             1047..1748
                     /locus_tag="VFO30_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HET9419420.1"
                     /translation="MLKYLFTIMVSLSLAQFGAAQTESKAGAAEKNWPTNALHAHVCG
                     IHFYSGDMKRQIIAEHYCSHWTDEILQCIIYDSNKPNARLIGVEYIVSAKIFESLPAE
                     EKKLWHSHNYEVKSGVLTAPEMADSAEKDLVKVLIGTYGKTWHMWQVDRGDKIPMGIP
                     QLMMAFTTDGQASPSILAEGDKLYGAAASKKTARADIPDAKIDPGADSWQKGQAMQLD
                     LKPVTVSAGSGASTQ"
     gene            1831..2799
                     /locus_tag="VFO30_08780"
     CDS             1831..2799
                     /locus_tag="VFO30_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET9419421.1"
                     /translation="MLEPVRPITLEEIHAARKRIANTVFRTPLVRLDLGPEFPDVQLK
                     LENLQPINAYKLRGAVNAVALLSETERKRGVWTISAGNAGQGVAYAAKEAGVTCTVVV
                     IETAPQSKLDRMRALGAKLVPVSYDAAWKALDERAFPGVEGTFVHPFDDHNFIAGHGT
                     LGLEILEDAPDTKAIVASIGGGGLITGVASAVKALKPDIKIFGVEPETAAPAALSQQA
                     GSPQVFKNWKASFVDGAGGQSMFPRMWQRMTGLVDDYFVVTLDDTIKAMRLMAEKARV
                     ISEGAGALPLAAILSDKIDNGPVVAIVSGGNIDLQKFCELIATSRD"
     gene            complement(2808..3404)
                     /locus_tag="VFO30_08785"
     CDS             complement(2808..3404)
                     /locus_tag="VFO30_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9419422.1"
                     /translation="MHPFMKSLWSIGIFAVAAIALFLCSCAPIPPEPQLSLSAAFPTP
                     APTPVGWWDGERGSGEPKIVVHIGEQKAYFYKGKHLLGVSVVSTGRPGFSTPPGQYQV
                     IGKDEHHVSTVFGDYVDAYGNIVKFNIDARKDGRPPGTHFDGARMPYALFFRSGYAMH
                     EGYVPPYAASHGCIRLPKGMAEHFFDAAPEGTTVIVKE"
     gene            3514..4098
                     /gene="nbaC"
                     /locus_tag="VFO30_08790"
     CDS             3514..4098
                     /gene="nbaC"
                     /locus_tag="VFO30_08790"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HET9419423.1"
                     /translation="MATLTEPTTSSSSQSNARPVVQPPIHLRSWIEANRDKFKPPVSN
                     QYLYDGRDLFVMVIKGPNARNDFHLVDSEEYFYQLQGDIKVRIRERDRTVDHIVREGE
                     TFFIPPNVPHSPQRPPDTIGVVVERRRPPGEKEHVIFYCENCGALVEDIHFDCADIVQ
                     HFSRAMLEFWNDDARRTCRNCGKKVEKPEPMQPF"
     gene            4186..5247
                     /locus_tag="VFO30_08795"
     CDS             4186..5247
                     /locus_tag="VFO30_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9419424.1"
                     /translation="MKIDIHTHILPRNWPDLDAKYGYNGFVRLEHSDPCTARMMIGDR
                     VFREITDNSWDPQRRLEQMHRSGVSMQVLSTVPVMFSYWAKPADALDLSQRLNDHIAE
                     VVREYPAHFTGLGTIPLQDVDLAAGELERCVRDLGLCGAEIGTHVDANEHCHGANCRN
                     LDDPALDVIWRAAEQLEVAIFVHPWDMIGQERMPKYWLPWLVGMPAETSLAICSMMFG
                     GVFERFPKLRVAFAHGGGAFPFSIGRIEQAFHARPDLVAIHNDTNPRKYLAQESAPAR
                     FYVDSLVHDPDALRMLLRLFGAKRVALGSDYPFPLGESNPGELIESMPDLSPVEKQQV
                     LAETGREFLGLESAVPAAR"
     gene            complement(5313..6098)
                     /locus_tag="VFO30_08800"
     CDS             complement(5313..6098)
                     /locus_tag="VFO30_08800"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET9419425.1"
                     /translation="MPPLTYANYLDLEKLLTLQNPRSSPPEHDEMLFIIIHQTYELWF
                     KQLLHEFEKINRDFSAGDLFGAIHSFKRTRTIMKTLVQQVDILETMTPKSFSSFRDRL
                     ETASGFQSVQFREIEFVLGYKRESALSHVKPDFPGADRLQRRVHQRTVVDHFYDFLET
                     RGAQIPAELKKRDVTKPNGPNEQVQKEILRLYKTAPEMSILFELMTDFDEGLQEWRYR
                     HIKLVERTIGAKKGTGGSPGVPFLKESLFKPIFHDLWAIRHEM"
     gene            complement(6343..6924)
                     /locus_tag="VFO30_08805"
     CDS             complement(6343..6924)
                     /locus_tag="VFO30_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9419426.1"
                     /translation="MTLAERALTILHEWVQSESLRKHCYAVADSMKHFAQLRGADIDA
                     WEAVGLLHDMDYERHPNLERSSTEGHPFVGVAWLRENGWNEEVCRAILSHADYSGVAR
                     ESPLEKTLYAVDELSSFVVAVALVRPTKNIHAVDLAAVKKKLKDKAFARAVSRQDINR
                     GATELGMPLDDVITNVIAALRSDAERLGLAGSV"
     gene            complement(7023..8024)
                     /locus_tag="VFO30_08810"
     CDS             complement(7023..8024)
                     /locus_tag="VFO30_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET9419427.1"
                     /translation="MFWAFVIPNLSRGAVALDALGSYLTQHGYGGAQLVPVGKFYHLP
                     VSANGKPGNLIIDTGSPATLIFRSSVKELGLTESKTTSHVRGAFGPSREFYGTATIKE
                     LKAGNCTLTNVPVAVAPVVSYGGRHANASGVLGLRELVKFAAILDLSHHLVYFTSSRP
                     NVQHFEAATWMRTKNGWRLVPPGSELSQMVRSILTEQGWTPVPFSIAHRHLRIAGAAN
                     GASCYFMLDTGSYLTLLDAAFAKRARIGDAPARIAAQGIGRSATDVRLAIVPAMRIGS
                     YEIKPASATVAVLDSEAIGRGTDSEVAGLLGIEHLAMNSAIFDFTSRQLYLRPRANH"
     gene            8211..8636
                     /locus_tag="VFO30_08815"
     CDS             8211..8636
                     /locus_tag="VFO30_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9419428.1"
                     /translation="MIKLLAFGAISLLAVAAPVFAAAERDAMMEKEKAIWQSVQDKKF
                     DGFRKYFASDYRAAYAQGIYNIDQEMDSVQKADLKSFSLSDSTVVMPDKDTALLTYKS
                     TTQGTQGGKDMSGTWNCASVWHKSGAEWKVVFHTEVKAQ"
     gene            8708..9583
                     /locus_tag="VFO30_08820"
     CDS             8708..9583
                     /locus_tag="VFO30_08820"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET9419429.1"
                     /translation="MRNARNEVSVRAGQIEFVKVNTDAWRWRDVYRWLLGLRWPQFAA
                     FVGAVYVALNLLFAALYSLNQNSIAGRTTHWFLDCFFFSVQTLATVGYGHMYPQTLYG
                     HVISTIEIMTGIFLLAVMTGLIFVRFSRPIARVVFSKPIVVAALNGKPTLMVRIGNEN
                     QHSMVEAEFRIMYSRDEPLLEGGDFRYFYVLKLHFDRLTVFPAALTLRHTIDEQSPLY
                     GATPESLQTERALFIVSVVGIDPVIAAAVQTQKDYHWRDVRFGERFVEIYTEQGGGRL
                     TVDYGRLHETEPAEL"
     gene            9644..10303
                     /locus_tag="VFO30_08825"
     CDS             9644..10303
                     /locus_tag="VFO30_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419430.1"
                     /translation="MAGQHNHLDKPEPRWQALLAVLAIGAIYLALPRNLIIGPIWLVP
                     TLIVLLLVPTVVSHRVGKRSLNRALGILINGITTVALIGSVGLLVRALPSHVGSPLAL
                     LRSGGLLWLTNVIVFALWYWRLDGGGPTRRHEEKKFGSTSFLFPQMQIPQDERTQFAC
                     ARWRPRFIDYLFVAFTQSSTFGPTDAPLLARWAKVLAMIQVFISLSIIILLISRAVGV
                     L"
     gene            10444..11148
                     /locus_tag="VFO30_08830"
     CDS             10444..11148
                     /locus_tag="VFO30_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9419431.1"
                     /translation="MNTRIGTSGFQYAEWKGNFYPEDLPASKMLPFYAERFSTTEINY
                     TFHRIPALKTIENWKTLTPEKFRFALKAPQKITHWSKLRDCSDTLEYFCKVVTGLGGR
                     LGPVLFQLPPTFRKDVDILNAFLRELPEMRAAFEFRHASWFDDELFELLKSRNVALCI
                     ADTADLATPKIITADYGYLRLRREDYKNLDIEAWAKFLREQKGIWHDVFVYFKHEESG
                     IGPKLAAEMIKLVVTS"
     gene            11145..11483
                     /locus_tag="VFO30_08835"
     CDS             11145..11483
                     /locus_tag="VFO30_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419432.1"
                     /translation="MNCRSRSFNRSFGFVGIALLFFVFYHSTMAQNASPAPQRDINAV
                     LAAHDRDLLALPDVVGVSVTTLDDHRTPCLKIMLARHNPKTERAIPRSIEGYPVIFKV
                     TGEIRALDQP"
     gene            11565..12263
                     /locus_tag="VFO30_08840"
     CDS             11565..12263
                     /locus_tag="VFO30_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9419433.1"
                     /translation="MATIAENLERVRAQMAEAAEKSGRNIADVELVAISKTHDAEKVR
                     EAVEAGQILFGESRVQEARIKIPELPSSLRWHFVGHLQKNKIRHALPLFELFHGVDNV
                     DLARQMDRIGQEEGMHPRVLLEVNVAGEGSKFGFNSEKLSEQMEELFGLPRLMIEGLM
                     CIPPLAKEAEASRKHFVQLRELRNALEKEFAVKLPQLSMGMTSDYAVAVEEGATLVRV
                     GTAIFGERKRNIDL"
     gene            12393..12707
                     /locus_tag="VFO30_08845"
     CDS             12393..12707
                     /locus_tag="VFO30_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET9419434.1"
                     /translation="MRLEPINIQQIGSELAIQWNDQTESYLNLEKLRRACPCAACGGE
                     PDVLGNISRPLVSYTNNSFQLASFNMVGGYALQPRWRDGHDTGLFTFQYLRRLATPVR
                     ST"
     gene            12704..12868
                     /locus_tag="VFO30_08850"
     CDS             12704..12868
                     /locus_tag="VFO30_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419435.1"
                     /translation="MSNGTKLFIIACCVALCAGAPIFVRTGPSTNPRAKSEVQPVNNQ
                     QSQRARWVQV"
     gene            complement(12923..13867)
                     /locus_tag="VFO30_08855"
     CDS             complement(12923..13867)
                     /locus_tag="VFO30_08855"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET9419436.1"
                     /translation="MSGSEIISPLIFEPILMERIWGGRKLAALFGKNIPAGKRVGESW
                     EIVDRPEAQSVVANGPLKGKTLHELWLQNRDSIFGNVPDAPRFPLLIKLLDAQDKLSL
                     QVHPPENIAAKLGGEPKTEFWYVAAADPGAEIFVGFRNSITREQFEKSLRAASVADHV
                     HSIRVKPGDAMFLSAGRFHAIGAGCVLVEIQQNSDTTYRLFDWNRKDDSGNRRPLHVD
                     EALQCIDFNDVAPGLIKPDGELLVRYELFEVQKWNVAAPREVTPRGQFAIVCCLTGMV
                     SCADVDLAPGKFCLIPASLQDRQLHARAEGTTLLRVTL"
     gene            13836..15452
                     /locus_tag="VFO30_08860"
     CDS             13836..15452
                     /locus_tag="VFO30_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9419437.1"
                     /translation="MSGDIISLPDIGSKRPTPNPPPQGYGAAGVQRSTLNSEAGRGGV
                     ADVRFERMSDWIETHIPARLDRLPWSAWHWFIVAALGATWILDGLEVTLAGALGGILT
                     RAETLGLSDAQVGASATFYLGGAVLGALGFGYATDRLGRKKLFFVTVGVYLTATALTA
                     FSWNFASYAFFRALTGAGIGGEYAAINSAIDELIPARVRGRVDLIINGSYWIGAALGS
                     AGSVIVLNPRLFPMALGWRLVFGIGALLGLVVIFFRQWIPESPRWLLIHGRAREAEEI
                     INQVERHAGVDACHAEAARRRVDFPTDAPPTRFRARKYTTFREIWDTIANKYRQRALL
                     GFVLMVSQAFFYNAIFFTYALVLMRFYDVPAQRVGGYLLPFALGNFLGPLLIGRFFDT
                     IGRKALIVATYAASGILLAFTGWLFREHLLTAPTQTIAWTVIFFVASAAASSAYLTVS
                     EIFPLEIRAFAIAVFYSIGTLAGGVFAPLIFGIIIGSGSREALFGGYLFGAALMIIAA
                     ATEAWIGVRAERQSLESIAAPLSSAEAEAY"
     gene            15449..>15709
                     /locus_tag="VFO30_08865"
     CDS             15449..>15709
                     /locus_tag="VFO30_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9419438.1"
                     /translation="MKTSARNFVTKWMPVIAWMLLIFAGSTDVLSAEHTSRFIVPFLR
                     WLDPTISIQAIIAIHFTLRKIGHFTEYAILAALLWRALRGTFA"
BASE COUNT         3257 a         4424 c         4630 g         3398 t
ORIGIN      
        1 gcatttaaag aagaatttcc ctatctgcgt gcggactcag gccaactttt cgagtttgac
       61 cgcgattggc tctatgccgc gattaatcgc gcagctgatg ccgccggtta cccgagctgg
      121 tggctaaccg atcacgtgac ggagagcatc gcgttttatc tgcgactgcg gaatgacgag
      181 aacgtggtcg cgttccgtca gctcgccgag acggtgcagt acgttttcaa agcaatcggc
      241 tacaaggaaa ttattccata tttcgcgccg gcgccgccgc cgatttcgct ttcgctgttg
      301 gagattgcgc acgccgccgg caccggttac gagctggcct ttttcgattt actcgagaaa
      361 cgcctcacgg cgctcattga gactggcgtg gacaacctgc ggttttcagg gctgcagcgc
      421 tgcgtcaaat tcgtgcgggg cgtcagggtg tggacacgtg ggtgtgacgt gttgcgcgag
      481 gagattgtgt gcttcattcg tgagcggcta acatcaacaa ctgccgagcg cctcaaatgt
      541 gaattgcgct gagccatgac catctttgag aaaattatcg cgcgcgaaat tccggcgaag
      601 atcatctggg aagacgacga ggcggttgcg tttcacgacg tcaatccgca ggcgccggtg
      661 cacgtgctca tcgtccccaa acgggtgatc ccacgactgg cggtggtcac agaagcggat
      721 cgtgcaactt tgggcaagct gctgctcgtc gcgggagagc tggcccggaa gctgggcatc
      781 gagcagaccg gctaccgcgt gatcatcaac agcgggccgg acgcgggcga aagcgtaccg
      841 catctacatg tccatttgtt aggcaaacgc gcgctggcct ggccgcccgg gtaaaatccg
      901 cgattgtaga ggcgctcgtc gcggcgagcg cacgttcgcc ggcggctgag gtcagccgcc
      961 gctacagctc cgcgcgccgg aacctgattg ttcggcgcca gccgcttctt ctataaccgg
     1021 gaccgagaat caaccaggag gaaattatgc tgaaatatct gttcaccatt atggttagct
     1081 tgagcctggc gcagttcgga gctgcgcaaa ctgaatcgaa agcgggcgcg gcggagaaaa
     1141 actggccgac gaatgcgctg cacgcgcatg tctgcgggat tcatttttac agcggtgaca
     1201 tgaagcgcca gatcattgcc gagcattatt gctcgcattg gaccgacgag atcctgcaat
     1261 gcatcatcta cgactcaaac aagccaaacg cgcggttgat cggcgtcgaa tacatcgtca
     1321 gcgcgaaaat tttcgagtcg ctgccggccg aggagaaaaa gctctggcac agtcacaatt
     1381 atgaggtgaa gtccggcgtg ctcaccgcgc cggaaatggc ggactcggcg gaaaaagatc
     1441 tggtcaaggt gttgatcggc acttacggca aaacctggca catgtggcag gttgatcgag
     1501 gcgacaaaat cccaatgggc attccgcaac tcatgatggc attcacgacg gacggccagg
     1561 cctcgcctag cattcttgcc gagggggaca agctctacgg cgccgccgcg tcgaaaaaga
     1621 cagcgcgcgc cgatattcct gacgcgaaga tcgatcccgg cgcggatagc tggcagaaag
     1681 gccaggccat gcagctcgac ctgaagcctg tcacggtgtc ggctgggtct ggagcgtcga
     1741 cccaataaga agttacgctg gccgtttgcg gctgaggcga gccgccgcta catagcgcgc
     1801 gggaatggat tgctgcatct gatgagcgcg atgcttgaac ctgttcgacc aatcacgctg
     1861 gaggaaatac atgccgcgcg caaacggatt gcgaacacgg tcttcagaac accgctcgtg
     1921 cggctggatc tgggtccgga gttcccagac gtgcagctga agctcgaaaa tctgcagccg
     1981 atcaacgcat ataaattgcg cggtgcagtg aatgccgtgg ccctccttag cgaaactgaa
     2041 cgcaagcgtg gggtgtggac gattagcgcg ggcaatgccg gccagggcgt tgcctacgcc
     2101 gcgaaagaag ccggtgtgac ctgcaccgtc gtcgttatcg aaacggcgcc gcaatctaag
     2161 ctcgacagaa tgcgggcact cggcgcgaag ttggttccgg tgtcgtacga tgccgcttgg
     2221 aaggcgctcg acgagcgcgc ctttcccgga gtggagggga cattcgtgca cccatttgat
     2281 gatcacaact tcatcgctgg acacggcacg ctcgggcttg agattctcga agacgcgccg
     2341 gatacgaagg caattgtcgc cagcattggc ggcggcggct tgatcaccgg cgttgcgagc
     2401 gccgtcaagg cgctcaagcc cgacatcaaa attttcggcg tggaaccgga aacggcggcg
     2461 ccggccgcgc tatcgcagca ggccggttcg ccgcaagtat tcaaaaattg gaaggcctca
     2521 ttcgtcgacg gcgccggggg ccaaagcatg ttcccgcgca tgtggcagcg gatgacaggt
     2581 ctggtggacg attatttcgt cgtaactctc gatgacacga taaaggcgat gcgactcatg
     2641 gccgaaaagg cacgggtcat ctccgaaggc gcaggcgcgc ttccgctagc tgcgatttta
     2701 agcgacaaga tcgacaatgg cccagtggtc gcgattgtgt cgggcggaaa catcgacctt
     2761 cagaaattct gcgagctgat cgcgacgtcc cgggattaag tcggcaatca ttctttgaca
     2821 atcacagtgg tgccttcggg cgccgcgtcg aaaaagtgct ccgccatgcc tttcggcaaa
     2881 cggatgcagc cgtgcgacgc cgcatacggc ggtacatacc cctcgtgcat ggcataaccg
     2941 ctgcggaaga acaacgcgta cggcatgcgc gcaccatcga agtgtgtgcc cggcggccgg
     3001 ccgtctttgc gggcgtcgat gttgaacttc acgatattac cgtaggcgtc cacgtagtct
     3061 ccgaaaacag ttgagacatg atgctcatcc ttgccgatga cctgatactg acccggcggc
     3121 gtggaaaagc ccggtctacc ggtcgaaacc accgagacgc cgaggagatg tttgcccttg
     3181 tagaaataag ccttctgttc gcctatgtgg acgacgattt tcggttcgcc ggagcccctt
     3241 tcgccgtccc accagccgac tggtgtgggc gctggtgtcg ggaacgccgc agagagtgat
     3301 agttgcggtt ccggcggaat gggcgcgcaa ctgcacagga agagcgcaat cgccgcgacg
     3361 gcgaaaattc ctatcgacca gagcgatttc atgaaggggt gcattttatg aggcgacgct
     3421 cgttaggtgc ttcaactgca aagcacgatg tggacctaat gtgccccgat gagttgaagt
     3481 tgacgcgtgc gcaaccgcac atacgctcgg ctcatggcta ctctgaccga gccgacaacc
     3541 agctcatcgt cgcaaagcaa cgcacgtccg gtcgtgcaac cgccgattca tctgcgcagc
     3601 tggatcgaag cgaaccgcga caaatttaag ccgcccgtca gcaatcagta tctttatgat
     3661 gggcgcgact tgtttgtcat ggtgattaag gggccaaacg cacgcaacga ttttcacctc
     3721 gtcgattcgg aggagtattt ctatcagctg cagggcgaca tcaaagtgcg catccgagaa
     3781 agggaccgca ccgtcgatca catcgtgcgc gagggtgaga cgttctttat tccgccgaat
     3841 gtgccgcatt cgccgcagcg gccgccggac acaatcgggg ttgtcgttga acgccgccgc
     3901 ccgcccggcg agaaggagca cgtgattttt tactgtgaga attgcggcgc attggttgaa
     3961 gacattcatt tcgactgcgc tgacattgtg cagcatttta gccgggccat gctcgaattt
     4021 tggaacgacg acgcgcggcg cacctgcaga aactgcggca aaaaagtcga aaagccggag
     4081 ccgatgcagc cgttctgacg aacggcagtg atgagtgaca ggtgacgagt gacgggtttc
     4141 cattagcttc gctgttcgtt tctcgtcact tactcgtcac tttccgtgaa gatcgacatc
     4201 cacacgcaca ttttgccgcg caattggccc gatctcgacg cgaaatacgg ctataacggt
     4261 ttcgttcgcc tggagcacag cgatccctgc acggcgcgca tgatgatcgg cgaccgagtg
     4321 ttccgcgaga tcacggacaa ctcgtgggat ccgcagcggc gcctcgagca aatgcatcgg
     4381 agtggggttt cgatgcaggt gttgtcgacc gtgccggtga tgttcagtta ctgggcgaaa
     4441 ccggccgacg cgctcgattt atcgcagcga ttgaatgatc acatcgcgga agtcgtgcgc
     4501 gagtacccgg cgcatttcac cggtctggga acgatcccgt tgcaagatgt cgatcttgcc
     4561 gcaggggaac tggagcggtg cgtgcgcgac ctcggcctct gcggcgcgga gattgggacg
     4621 cacgtggatg ctaacgaaca ttgtcacggc gcgaactgcc gcaatctcga cgatccggcg
     4681 ctggatgtaa tctggcgcgc cgcggagcag ctcgaagtgg ccatttttgt tcacccctgg
     4741 gatatgatcg gccaggaacg gatgccgaaa tactggctgc cgtggctggt cggcatgccg
     4801 gccgaaactt cactcgcgat ttgctcgatg atgttcggag gtgtgttcga acgtttcccg
     4861 aagttgcgcg tggctttcgc gcacggtggc ggcgctttcc ccttcagcat cgggcgcatc
     4921 gagcaagcat ttcatgcccg gcccgatctg gtcgccattc ataacgatac gaatccgaga
     4981 aaataccttg cccaagaaag cgcgccggcg cggttttacg tcgattcact cgtccacgat
     5041 ccggacgcat tgcgcatgtt gttgcgcttg tttggcgcga agcgcgtggc gttgggatcc
     5101 gattatccat ttccactcgg cgaatccaat ccaggcgaac ttatcgaatc gatgccagac
     5161 ctttcgccgg tcgaaaagca gcaggtcctg gccgaaaccg gacgcgagtt tctgggtctg
     5221 gagagcgccg tgcccgccgc acgttagctc ggcgtgcatg cagtcggctt aatttcatag
     5281 atgcgacggt gctctgccgt cgtcatctgt cgctacattt catgccggat agcccacaaa
     5341 tcgtggaaga tcggtttgaa aagcgattct ttcagaaacg gcaccccggg tgatccgccg
     5401 gtgcctttct tggcgccaat cgttcgttcg acaagtttga tatggcggta acgccactcc
     5461 tgcagccctt catcgaaatc ggtcatcaat tcgaacaaga tcgacatctc cggagccgtt
     5521 ttatagagcc gcagaatttc cttctggacc tgttcgttag gtccattcgg tttcgtgacg
     5581 tcgcgcttct ttagttccgc cgggatctgt gccccgcgcg tttcgagaaa atcatagaaa
     5641 tgatcgacca ccgtgcgttg atgcacgcgc cgctgcagcc gatccgcgcc ggggaaatcc
     5701 ggtttcacat gactcagcgc ggactcgcgt ttgtagccga gaacgaactc gatttcgcga
     5761 aattgcaccg attgaaatcc tgacgccgtc tcgagccggt cgcggaagct ggagaacgac
     5821 ttcggcgtca ttgtttcaag gatgtcgacc tgctgcacga gtgttttcat gatcgtgcgg
     5881 gtgcgtttga acgaatggat cgcgccgaag agatcacccg cggaaaagtc gcgatttatc
     5941 ttttcgaact catggagcaa ctgtttgaac caaagctcgt aggtctgatg aatgatgata
     6001 aagagcattt cgtcatgctc cggtgggctc gacctcggat tctgcaatgt gagcagtttc
     6061 tccaaatcga gataattggc gtaggtgagt gggggcatgg cgctttccca tattaaatcc
     6121 ggcgcgctcg ctgacaaagt cggattctgc tgcgccaccg atcggcacga aactcggcga
     6181 gggaatcaca gttgaggaga tgaaagaact gcgctggttg aaaccgaagc tcgtggcgca
     6241 aacgagtgga caaactacgg actgctgcgt cacgctactt tcgaaggatt gcgtgatgac
     6301 aaaactgcgg ccgaggtaac gcgcaaagtc gcggcgtgac agctaaacgg aacctgctag
     6361 accgaggcgc tccgcgtcgg agcgcaacgc ggcaatcacg tttgtgatta cgtcgtcgag
     6421 cggcatgccg agctcggtcg cgccgcggtt aatgtcctga cggctgacgg cacgggcgaa
     6481 tgctttatct ttgagcttct tttttacggc ggcaagatcg acagcgtgga tgttttttgt
     6541 tggcctaacg agtgccactg caacaacgaa actgctcagc tcatccacgg catagagagt
     6601 cttctcgagc ggcgattcgc gcgccacgcc ggaataatcg gcgtgagaga gaatcgcgcg
     6661 acaaacttct tcgttccagc cgttctcccg cagccaggca acgccgacga acgggtgacc
     6721 ttccgtcgag ctgcgttcca ggtttgggtg acgctcgtaa tccatgtcgt gcagcaggcc
     6781 aaccgcttcc catgcatcga tatctgcgcc gcgcagctgg gcaaaatgct tcatcgaatc
     6841 ggcgaccgca taacaatgtt tgcggagact ttccgattgc acccattcgt gcagaattgt
     6901 gagcgcgcgc tctgccaagg tcatggggac gaaatgccag aattagcaat agacgcagag
     6961 cggtgcgaca attggttcgt catcgagcgc gggcgaggga gttatcgctg ccgaagtgat
     7021 tatcagtggt tggcacgcgg ccgcaaataa agctgtctgg aagtgaaatc gaagatagcc
     7081 gaattcattg caaggtgctc gatcccgagc aaaccggcaa cttccgaatc ggtgccgcgg
     7141 ccgatcgctt ccgaatcaag gacagcgacg gttgcgctcg ccggtttgat ttcgtaactg
     7201 ccgatccgca tcgccggcac aatcgccaga cgaacgtcgg tagcggacct tccaatgcct
     7261 tgcgcggcaa tccgcgcggg cgcgtcaccg atccttgcgc gtttagcgaa ggcggcgtcc
     7321 agcagcgtca aatacgatcc ggtatccagc atgaaataac atgaggcgcc gttcgctgct
     7381 cctgcgattc gcaggtgcct gtgcgcgatc gaaaacggaa ctggcgtcca gccctgctcg
     7441 gtaagaatcg aacgcaccat ctgactcagt tcgctgcccg gcggcaccaa ccgccagccg
     7501 tttttggtgc gcatccacgt cgcggcttcg aaatgttgca cgttcggtcg cgatgaagtg
     7561 aaataaacca ggtgatgcga cagatccagg atcgccgcga atttcaccag ttcgcgtaag
     7621 cctaggacgc cactggcgtt tgcgtgccgg ccgccgtagc tgaccactgg agcaacggcc
     7681 acgggcacat ttgtcagagt gcaattgcca gccttcagtt ccttgatcgt agccgtgcca
     7741 tagaactcac ggctcgggcc aaatgcaccg cgcacgtggg atgtcgtctt cgactcggta
     7801 agaccgagct ctttcacgct cgagcggaaa atcagcgtag cgggtgagcc tgtgtcgatg
     7861 atgagattgc cgggcttgcc gttggcgctg accgggagat gatagaactt gccgacgggc
     7921 acaagctgcg ctccgccata tccgtgttgc gtcaaatagc tgccgagcgc atcgagtgcg
     7981 actgcgccac gcgaaagatt tgggatgaca aaagcccaaa acataaggac gccacaccgg
     8041 ccaaccttgt accgcatatc cgacctactc ggcgggattg tcgatcttac tcgcctctgc
     8101 gcaactgccg cttcgcctgc gatcgcccgt ggctctggcc gctaattaga agtattgacc
     8161 tgttcgcgct gcgatttacg ctggctgaca aattcaggag gagaaaaatt atgatcaagt
     8221 tgcttgcctt cggagccatt tcgttgcttg ccgtcgccgc accggtattc gccgccgccg
     8281 aaagggacgc catgatggag aaggagaaag ccatctggca aagcgtgcag gacaaaaagt
     8341 tcgacgggtt ccgcaaatat ttcgctagtg actatcgcgc cgcttacgcg cagggaatct
     8401 acaacatcga ccaggagatg gactccgtcc aaaaggccga cttgaaatcg ttctcgctca
     8461 gtgattccac tgtcgtcatg cccgacaagg acacggcgtt gctgacctac aaatcgacca
     8521 cgcaaggcac ccagggcggt aaagatatgt cgggcacgtg gaactgtgct tcggtctggc
     8581 acaagtccgg tgcggagtgg aaggttgtct ttcacaccga ggtaaaggcg caataggctg
     8641 ccgtcgatga attcgactcc ctcggcgacg accgcgggag gagcggctgc ctgacgaaga
     8701 agtgtcgatg cgaaacgcgc gtaacgaagt ttcggtccgg gcgggtcaga tcgagttcgt
     8761 caaagtcaac actgacgcct ggcgatggcg cgacgtttat cgctggctcc ttggtcttcg
     8821 gtggccgcag ttcgccgcat tcgtcggcgc agtttacgtc gctctgaatc ttcttttcgc
     8881 cgcgctctac agtctcaacc aaaacagcat cgcgggaagg accacgcatt ggttcctgga
     8941 ttgctttttc tttagcgtgc aaacgctcgc caccgtcggc tacggacaca tgtatccgca
     9001 gacgctctac ggacacgtga tcagcacgat cgaaatcatg accggaatat tcctgctcgc
     9061 ggtcatgacc ggcctcattt tcgtgcggtt ttcccggccg atcgcccggg tcgtgtttag
     9121 taaacccatt gtcgtcgccg cgctaaacgg caagccgacg ctgatggtgc gaatcggcaa
     9181 cgagaatcag cacagcatgg tcgaggccga gttccggatc atgtactcgc gtgatgaacc
     9241 gctgttggaa ggcggagatt tcagatactt ttacgttctc aaattgcatt tcgaccggct
     9301 gaccgtgttt ccggcggcgt taactttgcg acatacgatc gacgagcaaa gtccgcttta
     9361 cggcgcaact cccgaatcgc tccagacgga gcgcgcactt tttattgttt cggtagtagg
     9421 gattgacccg gtcatcgccg cggcggttca aacgcagaaa gattaccatt ggcgcgacgt
     9481 ccgcttcggc gagcgcttcg tcgagattta caccgaacag ggcggcggga gactaactgt
     9541 ggattacggg cggctccatg aaaccgagcc ggcggagttg tagctcataa cgagtgtcgc
     9601 gattgagttt cgcatcggcc cggtcacctg ctatagcgaa gtgatggccg ggcagcacaa
     9661 tcacctcgat aaaccagagc cgcgctggca ggcattgctc gccgtgctgg cgataggcgc
     9721 catttatctt gcgctgccgc gcaatcttat catcggtccg atttggctgg tgcccacttt
     9781 gatcgttctt ctgcttgtcc cgacagtggt gagtcaccgc gttggcaaac gctcgctgaa
     9841 ccgcgcgttg ggcatcctca ttaatggcat cacgacggtg gcgttgattg gatcggtcgg
     9901 gctgctggtg cgcgcgctgc cttcgcatgt gggatcgccg ctcgcgctat tgcgctctgg
     9961 tggactgctc tggctgacga acgtgatcgt ttttgccctc tggtactggc ggctggatgg
    10021 tggcggcccg acgcgacggc atgaggagaa aaaattcggc agcaccagtt ttcttttccc
    10081 gcaaatgcaa atcccgcagg atgaacgcac ccagtttgcc tgtgcgcggt ggcgaccgcg
    10141 ctttatcgat tatctcttcg ttgcctttac ccaaagctcg acttttggcc cgaccgacgc
    10201 gccgctgctc gcccgctggg caaaagtttt ggccatgatt caggttttca tttcactcag
    10261 tatcatcatt cttctcatct cccgcgcagt aggtgtgctg tgaagctgac gttaaatcgt
    10321 tgaaacgtta catcgttaca tcgattcacg actgacgctt ctgaattgga cgttgggcgt
    10381 taagcgcctg ccgcgccgta gccttgcgca ggcgggttga gcgttggaga ttttctgctc
    10441 ctgatgaaca ctcggatcgg cacctccggt tttcagtatg ccgaatggaa aggcaatttt
    10501 tacccggagg atctgccggc gagcaagatg ctcccgtttt acgcggagcg tttttccacg
    10561 accgagatca attacacctt ccaccgcatc ccggcgctga aaacgatcga gaactggaaa
    10621 acactgacgc cggagaaatt ccggttcgcg ctcaaggcgc cgcagaaaat cacacattgg
    10681 tcgaaattgc gcgattgctc cgacacgttg gaatatttct gcaaagtcgt aaccgggctc
    10741 gggggccgac ttgggccggt cctatttcag ttgccgccca ctttcaggaa agacgtcgat
    10801 atcttgaatg catttcttcg cgagctgccg gagatgcgcg cggcgtttga gttccgccac
    10861 gcctcctggt tcgacgatga actgttcgaa ctactcaagt ctcgaaacgt cgctctttgc
    10921 atcgccgaca cggccgacct tgcaactcca aaaatcatca ccgctgatta tggttatctt
    10981 cgactgcgtc gcgaagatta taagaacctc gacatcgaag cctgggcgaa attcctgcgg
    11041 gagcagaaag ggatttggca cgatgttttt gtttacttta aacacgagga gagcggcatc
    11101 ggaccgaagc ttgccgccga gatgatcaag ctcgtcgtaa catcatgaac tgcaggagta
    11161 gatcgttcaa tcggagcttc ggttttgtcg gcatcgcgct actcttcttt gtcttctacc
    11221 actcgaccat ggcgcaaaac gcttcgcccg cgcctcagcg cgacatcaat gccgtcctgg
    11281 ccgcacatga ccgagacttg ctcgccctgc cggatgttgt cggcgtttcc gttacgacgc
    11341 tcgatgatca ccgcacgccc tgcctgaaaa tcatgctcgc ccgacacaac ccgaaaacgg
    11401 agcgcgcgat tccgcgctcc atcgaaggct accctgttat cttcaaggtg accggcgaaa
    11461 ttcgcgctct cgaccaaccg tagccgcctc tgcagggcaa gcgcatcgct taccgttttc
    11521 atgtttggtt ttggcagacg cagcgcttgc cctacaattt tgctgtggca acgatcgcgg
    11581 aaaatctgga gcgcgtgcgt gcccaaatgg ccgaagcggc ggaaaaaagc ggtcgcaaca
    11641 ttgctgatgt cgaactcgtc gcgatttcta agacgcacga cgccgagaaa gtacgcgaag
    11701 cggtcgaagc gggacaaatt ttattcggtg aaagccgtgt gcaggaagcg cggataaaaa
    11761 tccctgagct gccgtcgagc ttgcgttggc atttcgttgg ccatctccag aagaataaaa
    11821 tccgtcacgc gctgccgctc ttcgaattat ttcatggcgt cgacaatgtc gatctggcgc
    11881 ggcagatgga ccgcatcggg caagaggagg ggatgcatcc gcgagtactt ctcgaagtca
    11941 atgttgcggg cgaggggagc aagttcgggt ttaactcgga gaaattgagt gagcaaatgg
    12001 aagagctctt cgggctgccg cggctcatga tcgaaggact gatgtgcatc ccgccgctgg
    12061 ccaaagaagc ggaagcatca cgaaaacatt ttgtgcaact tcgcgagttg cgtaacgcac
    12121 tcgaaaagga atttgccgtg aaactcccgc agctttcgat gggaatgacg agcgactacg
    12181 cggtcgccgt cgaagaaggc gcgacgcttg tgcgggtcgg cacggcgatt ttcggcgagc
    12241 gcaaacggaa catagacttg tagtctgtgc cgccagcgcg ttttaaaccc gctgagttga
    12301 acagcggaca aaatgtccgc tctgggcaca caggctggaa gcctatgttc cgattgctct
    12361 tgagaccatc gtgctgctaa gatcgacatc caatgcgcct ggagccgatc aacattcaac
    12421 aaatcgggag tgagctggca attcagtgga acgaccaaac tgagtcctac ttgaatttag
    12481 aaaagctgcg ccgtgcgtgt ccctgtgccg cgtgcggagg cgagccggac gtgcttggga
    12541 atatttcccg gccgctggtg agttacacta acaacagttt tcagctcgcg agcttcaaca
    12601 tggtcggcgg ctacgcgctg cagccgcgct ggcgcgacgg gcacgatacc ggcttattca
    12661 cctttcaata cctgcggagg ttagcgacgc cggtaagatc gacatgagca acggaacgaa
    12721 acttttcatc attgcttgct gtgtcgcgct ttgtgccggt gcgcctattt tcgtgcgaac
    12781 cggcccgtcg accaacccgc gagcgaaaag cgaagtgcag ccggtaaaca atcagcagtc
    12841 gcagcgagcg cgctgggtgc aggtgtagag gcgcttgtgc caagcgcctg tctatcagtt
    12901 ccggcgtttg gcacaaacgc cgctacaacg tcacccgcaa cagcgtcgtt ccctccgcgc
    12961 gcgcatgtaa ttgtcgatct tggagcgatg ctggaatgag acagaacttt cccggcgcaa
    13021 gatcgacatc ggcgcaactc accattccgg tgagacaaca gacaatcgca aactgaccgc
    13081 gcggcgtaac ttcgcgcggc gcagcgacat tccatttttg gacttcgaag agctcgtatc
    13141 tcaccagcag ctcaccgtcg ggtttgatta gtcccggcgc gacgtcgttg aagtcgatac
    13201 attgcaatgc ttcatcgaca tgcagcggtc gccggttgcc ggaatcgtct ttgcggttcc
    13261 aatcaaagag gcgatacgtc gtgtcgctgt tctgctgaat ctcaaccagc acgcaaccgg
    13321 cgccgatagc gtgaaaacga ccggccgaca gaaacatcgc atcgccgggt ttgacgcgga
    13381 tgctgtggac gtgatcggca actgatgcgg cgcggagtga tttttcgaat tgctcccgcg
    13441 tgatcgagtt tctgaagccg acgaaaatct ctgcgccagg atcagcggcg gcgacgtacc
    13501 aaaattcggt ttttggctcg ccaccgagtt tcgccgcgat gttttccggc ggatgaacct
    13561 gcaaagaaag tttgtcctgc gcatcgagca gtttgattag caacggaaag cgcggcgcgt
    13621 cggggacgtt gccgaaaatg gaatctcgat tttgcaacca cagctcgtgc aatgttttgc
    13681 ctttaagcgg accgttcgcg acgacgctct gcgcttctgg gcgatcgacg atttcccatg
    13741 attcgccgac gcgcttaccc gcaggaatgt tcttcccgaa cagcgccgcg agtttccggc
    13801 cgccccagat tcgctccatc aaaatcggct cgaagatgag cggagatata atttcgctgc
    13861 cggacatcgg cagcaaacgt ccgacgccca acccgcctcc gcaaggctac ggcgcggcag
    13921 gcgtccaacg ttcaacgctc aattcagaag ctggtcgcgg cggagtagcc gacgttagat
    13981 tcgagcggat gagcgattgg attgaaacgc acatcccggc gcggctcgac cggttgccgt
    14041 ggagcgcgtg gcactggttc atcgttgctg ccttgggcgc gacttggatt ctcgacggct
    14101 tggaggtgac cctggcgggc gcgctcggcg ggattcttac tcgcgcggag acgctcgggt
    14161 taagcgacgc gcaagtcggc gcgagcgcga ccttttacct cggcggcgcg gtgctcggcg
    14221 cacttgggtt cggttatgcg accgatcgcc ttgggcggaa gaaattgttc tttgtcacgg
    14281 ttggcgttta tctgaccgca actgcgttaa ctgctttctc ttggaacttc gcgagctatg
    14341 catttttccg cgcgctgact ggtgccggaa tcggcggtga atacgctgcg atcaactcgg
    14401 cgatcgacga attgatccct gcgcgcgtcc gtggtcgggt cgatctgatc atcaacggga
    14461 gctactggat tggcgcggcg cttggatcgg ccgggagcgt catcgttcta aatccgcggc
    14521 tctttccgat ggccttgggt tggcgtctgg tcttcggaat tggcgcgctc ctcggcttgg
    14581 tcgtcatctt tttccgacag tggatccctg agagtccgcg ctggctgctc attcatggcc
    14641 gcgcgcgaga agcggaagag atcatcaatc aagtcgaacg ccacgccgga gtagatgctt
    14701 gccacgccga agcagcgcgg aggcgggtcg atttcccaac cgatgcgccg ccgacgcggt
    14761 ttcgcgcgcg caaatacacg accttccgcg aaatctggga cacgatcgca aacaagtatc
    14821 gccaacgcgc gctgctcggc ttcgttctca tggtgtcgca ggcgttcttc tacaacgcga
    14881 tctttttcac gtatgcgctc gtcctgatgc gcttctacga tgtcccggcg caacgcgtcg
    14941 gtggctacct gttgccgttt gcgttgggca attttttggg accgctgctc attggtcgct
    15001 tctttgatac tatcggacgc aaggcgctga ttgtggcgac ctacgccgcg tcgggaattt
    15061 tgctcgcgtt taccggctgg ttgttccgcg aacacctgct gaccgcgcca acgcaaacca
    15121 tcgcgtggac cgtcatcttc tttgtcgctt ccgccgcggc cagctccgcc tacctcaccg
    15181 taagcgaaat tttcccgttg gagatccgcg ccttcgcgat cgccgtcttt tattccatcg
    15241 gaacgctcgc cggcggcgtg tttgcgccgc tcattttcgg gatcatcatc ggcagcgggt
    15301 cgcgcgaggc gcttttcggc gggtatcttt ttggcgcggc gctgatgatc atcgccgcag
    15361 cgactgaagc ctggatcggc gtgcgcgccg agcggcagtc tctcgagagc atagcggcgc
    15421 cattatcaag cgcggaggcc gaggcgtatt gaagacaagc gcgcgcaatt tcgtcactaa
    15481 gtggatgccg gtcatcgcgt ggatgttgtt aatttttgcc ggctcgaccg atgtgctctc
    15541 agccgagcac acgtcgcgtt ttatcgtgcc gttcttgcga tggctcgatc cgacaatttc
    15601 catccaggcg attatcgcga ttcacttcac tcttcgcaag atcggtcatt tcactgaata
    15661 cgcgatcctg gccgcgctat tatggcgggc attgcgcggc acattcgcc
//
LOCUS       DASTDG010000265         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1434134, whole genome shotgun sequence.
ACCESSION   DASTDG010000265 DASTDG010000000
VERSION     DASTDG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434134"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFO30_08870"
     CDS             complement(<1..260)
                     /locus_tag="VFO30_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419439.1"
                     /translation="MLEVALSDDAEVNVDVCRHCHFVWFDAREIDTLVPQPLPVPAPE
                     LSQEAREQIALAKVQELAAEARGSDFDSAPPDERWKQIVAFFG"
     gene            592..1986
                     /gene="mpl"
                     /locus_tag="VFO30_08875"
     CDS             592..1986
                     /gene="mpl"
                     /locus_tag="VFO30_08875"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET9419440.1"
                     /translation="MTKSDPRNFHFLGICGTAMGSVAAELRERGYVVTGSDENIYPPM
                     SLFLEERGIALNAGFRAENIPADADVVVIGNAMKRGNPEVEAVLNRKLFYLSLPEVLK
                     NYFLRGKHNLVVTGTHGKTTTTALLAWIMEKAGRKPGYLIGGIPKNVGQGARLNDSKY
                     FVIEGDEYDTAFFDKRSKFVHYLPELVIVNNIEFDHADIFRDVDEVKLSFRRLLNIVP
                     QNGMVLLNGDDHNCVEVARDCLAQLVEVGFSKNCAQRIRDVAYSASGSRFELGDEQFE
                     VPLSGEFNVRNAAMAATAARFYDVPKAKIDKALKTFRGVARRQEVRGEERGVTVIDDF
                     AHHPTALAQTLQALRHRFAKRRLWAVFEPRSNTTRRAVFQKELADALQLADGAFVAQV
                     ARLDQIPKHERLDPEAVVAAVAKSGRPAFYEPNADAIVERITSMLKPNDVVAVFSNGG
                     FDNIHEKLLARLRE"
BASE COUNT          448 a          589 c          657 g          405 t
ORIGIN      
        1 ccgaagaacg ccacgatctg tttccagcgt tcatcgggcg gcgcgctgtc aaagtcgctg
       61 ccgcgcgcct ccgcggcgag ttcttgaact ttggcgaggg cgatttgttc gcgagcctcc
      121 tgggacagtt ccggcgccgg cacgggcagc ggctgcggca cgagcgtatc tatttcgcga
      181 gcatcgaacc agacgaagtg gcaatggcgg caaacatcaa cattgacctc cgcatcgtcg
      241 gagagcgcca cttcgagcat tggctttcgg cacgcggggc agagtcgccc gcttgaacct
      301 tcgccgcgca aagcatggag ccagagtgga ttgatcgact caggcgtgaa agtccgccgc
      361 agaagctcga ctgtgacggc gcggccggtg caattgtcgc acccccaaaa aatgcggccg
      421 ctcattcgca cttgcttcag cggcaaattg caccgtggac aaatcagctc ggcatcgaca
      481 gccattgcac tggctcttgt tgcaatttca gagcctgatc gcgatttaaa acggaaggag
      541 cgcgtctctt ccgcattcgc aaaatctgag tgcgtgtcgt agagaatgcg cgtgacgaaa
      601 agcgatccgc ggaattttca tttcctcggc atttgcggca ccgcgatggg ctcggtggcg
      661 gcggaactgc gcgagcgtgg ctatgtcgtc accggttcgg acgagaacat ttacccgccg
      721 atgtcgctct ttctcgagga gcgagggatc gcgctgaacg ccgggttccg cgcggagaac
      781 attccggccg atgccgatgt ggtcgtaatc ggcaacgcga tgaagcgcgg caaccccgaa
      841 gtggaagcgg tgttaaaccg gaagcttttt tatctctcgc tgccggaagt cctgaagaac
      901 tacttcctcc gcggaaaaca caatctggtt gtcaccggca cgcacggcaa aaccacgacg
      961 accgcactgc tcgcgtggat catggaaaaa gcaggacgaa agcccggtta cttgatcggc
     1021 ggtattccga aaaatgtcgg ccaaggcgcg cgcctgaatg actcgaaata tttcgtgatc
     1081 gaaggcgacg agtacgacac ggcgtttttc gataagcgct cgaagttcgt ccactacctg
     1141 ccggaactgg tgattgtgaa caacatcgag ttcgaccacg cggatatctt tcgcgacgtc
     1201 gacgaagtga agctgagctt tcgccggctg ctcaacatcg tccctcaaaa cggaatggtg
     1261 ctattgaacg gcgatgacca caattgcgtg gaagtggcac gcgattgtct cgcgcaattg
     1321 gtcgaggtcg ggttttcgaa aaactgcgca caacggattc gcgacgtggc ttattcagcg
     1381 agtggctcgc gattcgaact cggtgacgaa cagtttgagg tcccgctgag cggcgagttc
     1441 aacgtccgca acgcggcgat ggctgcgacg gcggcgcgct tttacgacgt gccgaaagct
     1501 aagatcgaca aagcgctgaa aacatttcgg ggagtagcgc gtcggcagga agtacgcggc
     1561 gaagagcgtg gcgtcacggt gatcgacgat ttcgcgcatc acccgaccgc cctggcgcaa
     1621 acgctgcaag ccttgcgcca tcggtttgcg aagcgtcggc tctgggccgt tttcgagccg
     1681 cgctcgaaca cgacccggcg agctgtgttt cagaaggaac tcgccgatgc gctacagctg
     1741 gcggatggcg cgttcgtagc gcaggtggcg cgactggacc aaatcccgaa gcacgagcgc
     1801 ttggatccgg aagccgtggt cgcggcggtc gcgaaatcag gccggccggc gttttatgag
     1861 ccgaacgccg acgcgattgt tgagcgaatc acgtcgatgt tgaagccgaa cgacgtagtg
     1921 gcggtgttca gcaacggcgg attcgacaac atccacgaaa agctgctggc gcgcctgcgc
     1981 gagtgattgg ttaagcgtta acgcggattt acctgcccga tagtcgaccg cctaaacaat
     2041 tcaaggcgtc ggcggcggag cagctgacgt gtgatcgtgg acgatgcgcc agccttcgg
//
LOCUS       DASTDG010000266         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_495619, whole genome shotgun sequence.
ACCESSION   DASTDG010000266 DASTDG010000000
VERSION     DASTDG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495619"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFO30_08880"
     CDS             <1..180
                     /locus_tag="VFO30_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419441.1"
                     /translation="EKLWTYFAPMRKRRDEILRDKSYIDDVLARGAKRANAVADEVME
                     RVRTAVGISQRITKT"
     gene            complement(170..484)
                     /locus_tag="VFO30_08885"
     CDS             complement(170..484)
                     /locus_tag="VFO30_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET9419442.1"
                     /translation="MLPEVVESAHMNHPDFRVGGKIFATLGYPDADSAVVKLPPSQQG
                     ELVHDKPTVFAPAAGAWGRRGYTQLYLPKAKPPRVREALLKAWFNTAPKRLKARVRRP
                     TS"
     gene            complement(521..1540)
                     /locus_tag="VFO30_08890"
     CDS             complement(521..1540)
                     /locus_tag="VFO30_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9419443.1"
                     /translation="MTPLFTVNLLRVLFVTFCAAIGAIASTELQGGMWPGVVLGLLLG
                     LVVVLIDRLLKGFSLRAFSSATFGLLLGWIFARLLVASQIFAYLPGSTQWAIGLVVYC
                     TFGYLGMMLAMRSNRDEFSLIIPYVRFAREATQHEPVVVDTNVVIDGRIAELCATGFL
                     SRTLIVPRFVLDELQTLADSRDVLKRERGRRGLEILNNLQRAREVDLTIHESTAGEDT
                     ELGVDARLVRTAKMLQARLLTNDQSLCQVARLQQVPALNLTDLARALRPTLVSGDELQ
                     LNLVKEGREPHQAVGYLPDGTMIVVNHARDYLGKTASVIVSSALQTAAGRLIFAELKD
                     GANNR"
     gene            complement(1537..2430)
                     /locus_tag="VFO30_08895"
     CDS             complement(1537..2430)
                     /locus_tag="VFO30_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9419444.1"
                     /translation="MNRHERGLEFKVGIFVFFGLAMLGALVVQFGRLGEGFKTYYALT
                     VRFSDASGLLKGSDVLLAGAKIGKVAGGPRMVREGNGVDVPLKIYDYIKIPDGSKFTV
                     GSSGLLGDRFVNVTMPAGGRKAYLPPNAHISGARATGIDDLTREGGALVGDMRNAVQN
                     INGTFSRLNQEALSPANIENLKTSIAHLSETTGALAESSKKLDGVIDKADATMSSTKK
                     AADDLQRAVAEARKAIQMATEGKGLLAALLTNQQLANDLRALAGNLRAHGVLFYRDTA
                     AKAEKPAEQTKPSRPSSGERR"
     gene            complement(2505..3251)
                     /locus_tag="VFO30_08900"
     CDS             complement(2505..3251)
                     /locus_tag="VFO30_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9419445.1"
                     /translation="MIAVRGLVKRIGTEEILRGVDLEVARGETLAIIGRSGGGKSVLL
                     KHLIGLMRPDAGEIWVEGQNITTLNERQLASIRQKVGILFQGAALFDSMTVAENIAFP
                     LTEAGERDPDVLRTKVREMLEVMELEGEEDKMPVNLSGGMKKRVGLARSIIRQPSSIL
                     YDEPTSGLDPVVSDSINRLIRRLQRRFGVTSIVVTHDMKNAFHVADHIAYLHEGRIYF
                     HGTPTELQQSSDSVLQDFLLGRSEQGASAS"
     gene            complement(3311..4108)
                     /locus_tag="VFO30_08905"
     CDS             complement(3311..4108)
                     /locus_tag="VFO30_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9419446.1"
                     /translation="MIGSLFNALGGVVVRFIWYLGEVVLLAADTFRSIFTRRIRWKLF
                     LNQIVEIGLLSQLVVVITGAFTGAVFSAQTYFQFNKIGMGSAVGAVVSVAICRELGPV
                     LTALMVTGRVGAAMSAEIGTMKVTEQIDALRALAVYPTDYLVVPRALAMMVSMPLLVA
                     ECIAVGIAVGYLVAIFLLEVNGTYYMANMVRWTRNRDIIMGLSKAFCFGILIVFISCH
                     KGLTAREGAVGVGRATTEAVVNGSLAVLICNFFLTMTLNIIYPAGYQ"
BASE COUNT          923 a         1304 c         1246 g          883 t
ORIGIN      
        1 gagaagctct ggacctactt cgcgccgatg cgcaagcggc gcgacgaaat cctgcgcgac
       61 aaatcctaca tcgacgatgt gcttgcccgc ggcgcgaagc gggctaacgc cgtcgccgac
      121 gaggtgatgg agcgcgttcg aacggcggtc ggaatctcgc aacggatcac taagacgtag
      181 gacgacggac gcgagccttg agtcgctttg gcgccgtgtt gaaccacgct tttaaaagcg
      241 cctcgcgcac gcgagggggt tttgctttcg gcaaatacag ttgcgtatag ccgcgccggc
      301 cccaggcgcc tgcggcgggt gcgaatacag taggcttgtc gtgaacgagt tctccttgtt
      361 ggctcggcgg aagtttgacg acggccgaat ctgcatcggg atagccgagg gtggcgaata
      421 tctttccgcc cacacgaaaa tcggggtgat tcatatgagc cgactcgacg acctcgggca
      481 acatgagagc caacctcctg aactcgtccg gggtcaccat ttaccggttg ttagcgccat
      541 ctttgagctc ggcgaagatg agtcggccgg cggcagtttg cagcgcgctt gagacgatga
      601 cgctggcggt ctttccgaga tagtcacggg cgtggttaac cacgatcatc gtgccatccg
      661 gtaaatagcc gaccgcctgg tgcggttcgc ggccttcctt caccagattc agttgcagct
      721 cgtcgccgct gacaagcgtc ggccgcaatg cgcgggcgag atcggtgagg ttgagtgccg
      781 gcacctgctg caaacgcgcg acctggcaaa gggattgatc gttcgtgagg agccgcgctt
      841 gcaacatctt tgccgtgcgc actaaccgcg cgtccactcc cagctcggtg tcttcgcccg
      901 ctgtgctttc gtggatggtt aaatcgacct cgcgcgcgcg ttgcaggttg ttcagaatct
      961 cgagaccccg ccggccgcgc tcacgtttca gcacatcacg cgaatcggcc agagtttgca
     1021 gttcatccag cacaaagcgc ggcacgatca gggtgcgact gaggaaaccg gtcgcgcaaa
     1081 gctccgcgat gcgtccatcg atgaccacgt tcgtgtccac caccaccggt tcgtgttgcg
     1141 tcgcctcacg ggcgaaacgc acataaggaa tgatgagcga gaattcgtca cgattgctgc
     1201 gcatcgccag catcatccca agataaccaa acgtgcaata gacaaccaac ccgatcgccc
     1261 actgcgtgct gccgggcaga taggcgaaaa tctgcgaggc aaccaggagt cgagcgaaaa
     1321 tccatccaag cagcagacca aaagtggccg acgaaaacgc gcggagcgaa aagcctttga
     1381 gcaggcgatc gatcagcaca acgaccagcc cgagaagcaa accgaggaca acgcccggcc
     1441 acatgcctcc ctgcaattcc gtggaagcaa tcgcgccgat ggcggcgcag aacgtcacga
     1501 ataggacgcg cagcaggttg accgtaaaga gtggcgtcat cggcgttctc cgctgctcgg
     1561 gcgcgacggt ttggtttgct cggccggttt ttccgctttc gcggcggtat cgcgataaaa
     1621 caaaacgccg tgtgcgcgca gattgccggc aagcgcgcgc agatcattcg ccaactgctg
     1681 attggtgagc agcgccgcca gtaacccttt gccttcggtc gccatttgga tggcctttcg
     1741 cgcttcagcg acggcgcgct gtaaatcgtc ggccgccttt ttcgtcgagc tcatcgtcgc
     1801 gtcggctttg tcgatcacgc cgtcgagttt tttggacgat tcggccagcg cgccggtcgt
     1861 ctcactcaga tgcgcgatgc tcgttttcag gttctcgatg ttggctggcg agagcgcctc
     1921 ctggttcaag cggctaaacg ttccgttgat attttgcacc gcgttacgca tgtcgccgac
     1981 caacgcgccg ccttcgcggg tgagatcgtc gataccggtc gcgcgcgcgc cgctgatatg
     2041 cgcgttcggc ggcaaatacg ctttgcgccc gccggccggc atcgtcacgt tgacaaaccg
     2101 gtcgcccagc aaaccggaac tgccgaccgt gaatttgctg ccatcgggaa tctttatgta
     2161 gtcataaatc ttcaggggta catcgacgcc gttgccttcg cgaaccatcc gaggaccgcc
     2221 ggccactttt ccaattttcg cgccggccag caaaacatca gaacctttca ggagaccgct
     2281 cgcatcgctg aagcgaacgg tgagggcata ataagtttta aatccttccc ccagcctacc
     2341 gaattgcacg acgagcgcgc cgagcatcgc cagcccgaaa aaaacaaaga tgcctacctt
     2401 gaattccaga cctcgctcgt gccggttcat tgctaaaagt gattgtcgat cttaccgggg
     2461 ttttggcaag gagcggcgct ctccagagcg ccctctttcg gcggctagga agccgacgct
     2521 ccttgttccg agcgcccgag caaaaaatcc tgaagcaccg agtcggacga ctgctgcaac
     2581 tcggtgggcg tgccgtgaaa ataaatccgg ccctcatgca ggtacgcgat gtgatcggca
     2641 acatgaaacg cgtttttcat gtcgtgagtc accacgatgc tggtaacgcc gaatcgccgc
     2701 tgcaatcggc ggatcaaccg gttgattgaa tccgatacca ccggatcgag tcccgaggtg
     2761 ggctcgtcat aaagaatgga cgagggctgc cgaatgatcg aacgcgcgag gccaacccgc
     2821 ttcttcatgc cgccgctcaa attcaccggc attttgtctt cctcgccttc gagttccatg
     2881 acttcgagca tttcccgcac tttcgtgcgg agcacgtcgg ggtcgcgctc gccggcttcg
     2941 gttaatggaa aggcgatgtt ttccgctacg gtcatcgaat cgaacaaggc ggcgccttga
     3001 aaaagaatgc ccaccttctg gcgaatcgac gcaagctggc gctcgtttag cgtggtaatg
     3061 ttttgacctt ctacccaaat ttcgcccgca tccggacgca tcaaaccgat cagatgtttc
     3121 agcaaaacgc ttttgccgcc accgctgcgg ccgatgatcg ccagggtctc gccgcgtgct
     3181 acttcgagat cgacgccacg caaaatttct tccgtgccga ttcgtttcac cagcccgcgc
     3241 accgcaatca tggaaccatt atctcgatcc ggtgatggcg tcgcggcggt tggcggttgt
     3301 gcgttctcga tcattgatac cccgccggat aaatgatgtt gagcgtcatg gtgaggaaaa
     3361 aattacagat cagaacggcc agcgaaccgt tcacgaccgc ctcggtggtg gcgcgaccga
     3421 caccgaccgc gccctcgcgc gcggtcaacc ctttgtgaca actaatgaag acgatcagga
     3481 ttccgaaaca gaacgctttc gacagtccca tgatgatgtc gcggttacgc gtccagcgca
     3541 ccatgttcgc catgtagtaa gtgccgttca cttcgagcag gaagatcgcc accaaataac
     3601 cgactgcgat ccctacggca atgcattcgg cgaccaacag cggcatcgac accatcatgg
     3661 cgagcgcgcg cggcaccacg aggtaatcgg tcggataaac cgccagcgcg cgtaacgcgt
     3721 caatctgctc ggtcaccttc atcgtcccga tttcggccga catggctgcg cccactcgcc
     3781 ccgtgaccat cagcgccgtc aacaccggac ccagttcgcg gcagattgcc accgagacga
     3841 ctgcacccac ggctgagccc atgccaatct tattgaactg aaaataggtt tgcgccgaga
     3901 agaccgcgcc ggtgaatgcg ccggtaatca ctacgaccaa ttgggagagc aaaccgatct
     3961 cgacgatttg attgagaaac agcttccagc ggatccgtcg ggtgaagatc gagcgaaagg
     4021 tgtccgcggc caggagaacg acttcgccga ggtaccaaat gaatctaacg acaacacctc
     4081 cgagcgcgtt gaagagcgaa ccaatcatta cgtccgcaca gtactgaccg acgcggtgcg
     4141 gctcaaatgc tcggtgttgg gtctttgttt tttgggcgca ggtccgacag gatcgcgcgg
     4201 atgaaacctc ctttggtcaa gcctcttcaa gatcggtaaa tgtggctgtt ttagcgtgtc
     4261 ttgcgcgggg tgcggacatg agtagatgtc gatcttgcaa tgaaaagcgc gcttcgagtt
     4321 ctcttttggc ttcaggtcgt tgcaagcgca ctcgtt
//
LOCUS       DASTDG010000267         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1233741, whole genome shotgun sequence.
ACCESSION   DASTDG010000267 DASTDG010000000
VERSION     DASTDG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233741"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..657
                     /gene="rplM"
                     /locus_tag="VFO30_08910"
     CDS             226..657
                     /gene="rplM"
                     /locus_tag="VFO30_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9419447.1"
                     /translation="MKTFSAKGREVPRKWWVIDAKDQVLGLVAVKAANLLRGKEKVIF
                     TPHVDTGDFVIVINADKVRLTGKKEDQKSYMSFSGYVGGHKSETVRARRARHPELLIE
                     RAVRGMIPHNRLGRAVYRKLKVYRGDSHPHAAQQPQPVKVA"
     gene            666..1061
                     /gene="rpsI"
                     /locus_tag="VFO30_08915"
     CDS             666..1061
                     /gene="rpsI"
                     /locus_tag="VFO30_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9419448.1"
                     /translation="MAQTQEFIATGRRKNSVARIRMTPGSGKIDINGRSFEDYFPTAP
                     LQNTVLQPLQTAKLVNVYDLSINASGGGVAGQAGAARLAIARALLQADADLRASLKAD
                     GLLRRDPRMKERKKSGQPGARKRFQFSKR"
     gene            complement(1147..1971)
                     /locus_tag="VFO30_08920"
     CDS             complement(1147..1971)
                     /locus_tag="VFO30_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9419449.1"
                     /translation="MDFQLLREKVPRGIKTTLAPLSVSAASTIIHMPTAPPPSRDAAL
                     ETRFFWETHKTEIVAVLLLVLLAIVGYGAYRFYSDRRDAAAAALLARAKTTRDFEQVV
                     SAYPNTPAGANAYLLLADAQRNERKFAESNATLQAFIAKYPKHELVTSAWMAIAANYE
                     SLGKMDEALATYEKAAASDPDNFNTPLALLSQVHLLKAKNKTEEARIVCEKILTNYRE
                     SVWANEAMRQLRLLKPPTPAAAASSPTGPAQPAPPPLLARPAAPPVPSAAPQPKKP"
     gene            2000..2215
                     /locus_tag="VFO30_08925"
     CDS             2000..2215
                     /locus_tag="VFO30_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HET9419450.1"
                     /translation="MNPDEPTPAMQNPEKDPQDWTTGEEPMTGPQRSYLQTLCREAGE
                     EFNDKLTKAEASKKIEELQAKTGRGKK"
     gene            2870..3379
                     /locus_tag="VFO30_08930"
     CDS             2870..3379
                     /locus_tag="VFO30_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET9419451.1"
                     /translation="MNVFYDFVTALLGLGLEPKELTFVQVSLRGVIIFFFTLVMVRLS
                     SKRSLAEKTAFDAILLVILASVLSRAINGTAGFFTSIGCSFVLVFLHRFFAWIACRSH
                     AFGKIIKGCPAVIIQNGQVDLAVMRRNQISQHDLEEDLRLEAKTEDLAKIKVARLERS
                     GDVSFIKAE"
     gene            3465..4556
                     /locus_tag="VFO30_08935"
     CDS             3465..4556
                     /locus_tag="VFO30_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET9419452.1"
                     /translation="MIPHDKLFTPGPVEVSPKTLAAFSRPMIAHRGDDFKNLYRDIHP
                     KLQTLFGTKQPVFLSTSSAWGVMEAAIRNLVCNNRRSGSGLLCCMCGAFSDKWLDVAR
                     RCGKTAEPLQIEWGKHIDHKDVDLALASGKFDTVTLVHNETSTGVMNPLPEICCTLAK
                     YPDVILIVDTVSSFSAMKIDMDALGIDLMLTGAQKALALPPGFSLFSVSEKAFTRAEK
                     VKERGYYFDFLEFKKQQAEWMTPSTPSSGHIFALQSKLGEIFEEGLTNRYERHARTNA
                     LVHDWVRSRGLDFFAEEGYRSLTLTCVKNNKGIDVLDFARQLREKHHLVIDPGYGKLR
                     GKTFRLSNMGDETEETISHLLTCVEDCLA"
     gene            complement(4647..>5246)
                     /locus_tag="VFO30_08940"
     CDS             complement(4647..>5246)
                     /locus_tag="VFO30_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9419453.1"
                     /translation="GDPNSTLAKNAHVFLDVSIEQEACPLNLAPTSSTTVMLALGDAL
                     AMVLLEARGFNKEDFAKFHPGGMIGRSLLMRVHQIMRPREAMALVSPQATIREVLRAM
                     TSVRAGAAIVAGEDRQLLGIFTHGDFVRYFQNDPKIGERLVADLMTLNPVSVHQDKLA
                     VEVLNLLEQHRIDDLVVVDDNNVPVGIVDSQDLTRLKLL"
BASE COUNT         1062 a         1544 c         1494 g         1146 t
ORIGIN      
        1 acggtaaaaa cagcccggga aaaaatgcgc caggccggca aggaatttca cgacgcgatc
       61 gaagcggcca tgctcaaagc cgacccgacg attaagccgg tgctcgacaa gctcccgaaa
      121 ggcccagccc cggccgagca cgaggattaa tccgccaaac tgactcaccc gatgagttga
      181 cactgcaaca ggctatcagt agcttcgctg cccttttttc cgacgatgaa aaccttttct
      241 gccaaaggcc gcgaagtccc gcgcaagtgg tgggtcattg acgcaaaaga tcaggtgctc
      301 ggcctcgtcg cggtcaaggc cgcgaacctg ctgcgcggca aagaaaaagt gatttttact
      361 ccgcacgtgg ataccggcga cttcgtcatc gttatcaacg ccgacaaagt gcggttgacc
      421 gggaaaaaag aagaccagaa aagctacatg agcttttccg gttacgtcgg cggccataaa
      481 tccgaaacgg tgcgcgcccg gcgcgcgcga catcccgagc tgctgatcga acgcgcggtg
      541 cgcgggatga ttccacataa ccgcctcggc cgcgccgtgt atcggaagtt aaaggtctac
      601 cgcggcgact cgcatccgca cgccgcgcaa cagccgcagc ccgtgaaggt ggcctagcga
      661 actctatggc gcagacacag gagtttattg ccaccgggcg acggaaaaat tccgtcgcgc
      721 gcattcgcat gacaccgggt agcggcaaga tcgacatcaa cggccgcagc ttcgaagact
      781 attttcccac tgcgccattg cagaataccg ttttgcagcc gttgcaaacg gcgaagctag
      841 tgaacgttta cgatttgtcg attaacgcca gcggcggcgg cgtggcgggc caagccggcg
      901 ccgcgcgctt ggcgatcgcg cgcgcgcttc tgcaggctga cgcggacctg cgcgcgagcc
      961 tgaaagccga cggcctgctt cgtcgcgatc cgcggatgaa agagcggaag aagtctggtc
     1021 aacctggcgc gcgcaaacgc ttccagttct cgaaacggta attggttttc tgtagcggca
     1081 gtctgtgact gtcgccgaag ttaaggcccg aagacaatgc gacggtcata gaccgccgcc
     1141 acaaaatcac ggttttttcg gctgtggcgc agcgcttggc actgggggcg cggccgggcg
     1201 ggcgagcagc ggcggcggcg ccggttgcgc cgggccagtg ggagaacttg ctgcggccgc
     1261 gggtgtcggc ggtttcagca aacgcaactg acgcatggct tcgtttgccc acacgctctc
     1321 gcggtaattg gtcaggattt tctcgcagac gatgcgtgct tcttcggttt tgttttttgc
     1381 cttcaacagg tgcacctgcg atagcagcgc cagcggcgta ttgaaattgt ccggatcact
     1441 cgccgcggcc ttctcatagg tcgccagcgc ctcgtccatt ttgcccagcg actcgtaatt
     1501 tgcagcaatc gccatccacg cgcttgtcac tagctcgtgc tttggatatt tcgcgatgaa
     1561 agcttggagc gtggcgttcg attcggcgaa cttccgttcg tttcgctgcg cgtccgctaa
     1621 gagcaagtag gcattcgcac ccgcgggcgt gttcgggtat gcggaaacaa cctgttcaaa
     1681 atctcgcgtc gtttttgccc gggcgagtaa agccgcagca gcggcatccc gacggtccga
     1741 ataaaatctg tatgcgccat aaccgacaat tgccagcaac acgagcagaa gcacagcgac
     1801 gatctccgtc ttgtgcgttt cccagaaaaa gcgcgtctcg agggcggcat cacgggacgg
     1861 tggcggggct gtcggcatgt gaatgattgt cgatgctgcg gacactgata atggcgcgag
     1921 cgtcgtctta atgccacgcg gaactttttc gcgcaagagt tggaagtcca cgccgcaccg
     1981 ggcagacgat ttcattgtga tgaatcccga cgaaccgacg ccggcaatgc agaacccgga
     2041 gaaagatccg caagactgga cgaccggcga ggagccaatg accggaccgc agcgttccta
     2101 tctgcaaacg ctttgccggg aagctggcga agagttcaac gataaactca caaaagctga
     2161 agcctcaaaa aagatcgaag agcttcaggc gaaaaccggc cgcggcaaga agtaggttcc
     2221 gtgtaatgga gggacgacct ccgtgtcgtc ccattaacgg ccgcgcttac ttttagcttt
     2281 gcttcggccg cgctggttgg gcgcgctgcc cgcggcgctg cctccgccgc gttccaaatt
     2341 tgtgccgctc ggttttttac ccaagctgcg ccgttcgccg ggccgatttc caggacgagc
     2401 gccggttttg gtgcgtgcgc cacggccgtc ggcgtccggc acgactagct ctgatttttc
     2461 cttgtgcgac atcgaaggct tgtcctgagc gaagtcgaat ggctcgcaat caatgccgct
     2521 tctttttgcc cccgcctttt cctccgcctt tacgcgagct cgatttgctt cgcttcttgc
     2581 cgcggccacc gccgcctttt tttcttccgc ctttgtcgga cttgttgacc gtcgcccagg
     2641 cgcggcgctc ggcttccttg ctgcttacgc cgcgtttctt gtacccgcgc tcaatcttgg
     2701 atgcttttcg tttttgcttc gacgtgtatt tctttttgct ccctcgggac cataaattcc
     2761 tcctcgttcc tccgtaccta gaacgcagtc gcccgactcg gcggactttc gtttacaggg
     2821 aagatcgaca tacactcgtc ggtctttttt gccgcgatca atttttgtta tgaatgtgtt
     2881 ctatgatttt gttacagcgc ttctcggatt ggggctcgag ccgaaagagc tcacttttgt
     2941 acaggtttcg ctgcgcggtg tgattatttt cttcttcact ctggtgatgg tgcgtttgag
     3001 cagcaaacgt tcgctcgcgg aaaaaacagc ctttgacgcg attctactgg tcatcctcgc
     3061 ttcggttctt tcgcgcgcga ttaatggcac cgccgggttt ttcacctcga tcggttgcag
     3121 cttcgtcctg gtttttctcc atcgcttttt cgcatggatc gcctgccgct cacacgcttt
     3181 tggcaagatc attaaaggtt gtcccgccgt cattattcaa aacggacaag tcgatctggc
     3241 ggtaatgcga cgtaaccaga tttcccagca cgacctcgaa gaagatttgc gcttggaagc
     3301 gaagacggag gaccttgcca aaataaaagt cgcgcgcctc gaacgcagcg gcgatgtcag
     3361 cttcatcaaa gccgagtgaa ttgtagctgc tgccgccgtc tctggcggca gaactctgca
     3421 tttcaacgtt ggacgttggg cgttcgacgt tggacgtttg cttcgtgatt ccgcacgata
     3481 agcttttcac tcccggaccg gtcgaggttt cgcccaaaac tctcgccgcg ttctcgcggc
     3541 cgatgatcgc gcaccgcggc gatgatttca aaaatctgta ccgcgacatt cacccgaagc
     3601 tgcaaacgct cttcggcaca aaacagccgg tctttctctc cacgtcatcg gcttggggcg
     3661 taatggaagc ggcgatccga aatctcgtct gcaacaaccg ccggtccggc tccggactgc
     3721 tttgttgtat gtgcggcgcg ttttccgaca aatggctcga cgtggcgcgc cgctgcggca
     3781 aaaccgccga gccgctccag atcgaatggg gcaagcacat cgaccataaa gatgtcgatc
     3841 ttgcactcgc ctcgggaaaa ttcgacaccg tcacgctcgt tcacaacgag acctccaccg
     3901 gtgtcatgaa tccgctaccg gaaatttgct gcacgctcgc caaatacccg gatgtcattc
     3961 tgatcgtcga caccgtctcg tcgttttccg ccatgaagat cgacatggac gcgctcggga
     4021 ttgacctcat gctcaccggc gcgcaaaaag cgctcgcgtt gccgccgggg ttttcgctct
     4081 tcagcgtgtc ggaaaaggcc tttacccgcg ccgagaaggt gaaggaacgc ggctactact
     4141 tcgattttct cgagttcaaa aaacaacagg ctgaatggat gacacctagc accccgagca
     4201 gcggccacat cttcgccctg caatcaaagc tcggcgaaat cttcgaagaa ggcctaacga
     4261 atcgctacga acgccacgcc cgcaccaacg cgcttgtgca cgactgggtc cgtagccgcg
     4321 gcttggactt cttcgccgaa gaaggctacc gctcgctcac gctcacatgc gtgaagaaca
     4381 acaaaggcat cgacgttctc gattttgcgc gacaactgcg cgaaaaacat cacctcgtca
     4441 tcgaccccgg ctacggaaaa cttcgcggca aaacatttcg cctatccaac atgggcgacg
     4501 aaaccgaaga gaccatttcg catctgctca cttgtgtaga ggattgcctc gcgtagcgca
     4561 cgcgtctcgc gtgctagttt tgatgtactg ccaaaactac tgatgcgcgg cttggttcga
     4621 cttcacaatc gagctatgcg gcggcttcag agcagcttta gccgggtcag gtcctgggag
     4681 tcgacgatgc cgaccgggac attgttgtcg tcgaccacga cgaggtcgtc gatgcgatgt
     4741 tgttcgagaa ggttgaggac ttcgacggcg agtttgtcct ggtgaacgct gacgggattg
     4801 agcgtcatga gatcggcgac gagacgttcg ccgattttcg ggtcgttctg aaaatagcgg
     4861 acgaaatcgc catgggtgaa gatgccgagt aattgtcgat cttcgccggc gacgatggcg
     4921 gcgccggcac ggacgctcgt catcgcgcgt agaacttcgc ggatcgtcgc ctgcggggat
     4981 acgagcgcca tcgcttcgcg cggccgcatg atctggtgca cgcgcatgag caggctgcgc
     5041 ccgatcatgc cgccgggatg aaacttggcg aaatcttcct tgttaaagcc gcgtgcctca
     5101 agcaacacca tcgccagcgc gtcgccgagc gcgagcatga cggtcgtgct cgaagttggg
     5161 gcgaggttca gcggacacgc ttcctgctcg atgctcacgt caaggaacac gtgcgcgttt
     5221 ttcgcgagcg tcgagttcgg gtcgcc
//
LOCUS       DASTDG010000268        11624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_1500114, whole genome shotgun sequence.
ACCESSION   DASTDG010000268 DASTDG010000000
VERSION     DASTDG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500114"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..877
                     /gene="panB"
                     /locus_tag="VFO30_08945"
     CDS             107..877
                     /gene="panB"
                     /locus_tag="VFO30_08945"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9419454.1"
                     /translation="MLNVTEKFRAMKQRGEKITALTAYDYPTARLLDESGIDIVLVGD
                     SLGMVVLGYEDTTRVTLEEMLHHTRAVARGVTRALLVADMPIHTYDKPEQAVATAQKF
                     VEAGAQCVKLEGGASHGAQIKAIRAAGIPFMAHIGMLPQSVREEGGYKVKGRTQSEAE
                     ALLADARAVESAGAFSVVLEIVIADLAWQISQAISIPTIGIGSGAHCDGQILVTHDLI
                     GLFPWFTPKFVSPEARVADEIRKAVRAFIERTRSARPV"
     gene            987..2027
                     /locus_tag="VFO30_08950"
     CDS             987..2027
                     /locus_tag="VFO30_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9419455.1"
                     /translation="MRVSNFDQFDELDYNRALLSGTGERNWLWLGLIGSLALHLALCA
                     YFYRARFQSVESVVVPNQPTITFKVKNVDLAQLEKSSSDQTSAAAKPEPNSPTDQQPD
                     EKKAFDKLLQEVQASTAMPDDIQNVLPDKPKVEPDITSVLPDIERSTAQTLSNSPNAT
                     HEQSAPNDTAVSGRPQPALSGTELATSTSIKRPSTFTSKLPADSAGPERGRAGFSDLD
                     QLLAQKGPLGSGTKLQLPDDQLFEYDSAELRPSDLLSKVATLIRRNPKATFTVEGYTD
                     SFGTPEYNLDLSQRRADSVKQYLVQVMGISPDQIEARGYGQTKFRVSPNGSIGEQREN
                     RRVEIVVHTNEP"
     gene            complement(2062..2658)
                     /locus_tag="VFO30_08955"
     CDS             complement(2062..2658)
                     /locus_tag="VFO30_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9419456.1"
                     /translation="MIVAQLFKLRIRGAQIGNSRHIGGFTLVELLVVITIIIILAALV
                     LATVGYVQKKGARSRTEAEIAAMSAACESYKADNGIYPSNSDTNALNARSNFDASASA
                     YSAASLYLYEQLAGVTSGNRSETSSSRTYFIFKPNMLLPAPPSTADVTAIRDPFGNSY
                     GYSTIQAATGDSTKGYNPTFDLWSTVNSTDPTQWIKNW"
     gene            complement(2655..3953)
                     /locus_tag="VFO30_08960"
     CDS             complement(2655..3953)
                     /locus_tag="VFO30_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9419457.1"
                     /translation="MPRFAYVALDGRGQESTGSVEASSSNEAIGQLRRAGFFPTSVYE
                     EAKAGGRDGKKPARKTRVKSLAAPRGKGLRMEITLFRRKKVKAKTLMIFTRQLATLID
                     SGLPLLRGLNVLARQERDATLKSAIESLANSVQGGSTFSDSLAQHPSIFNELYVNMVK
                     AGELGGVLELVLGRLAEFQEKAQKIKNKVAAAMVYPIIVMTMAVGIMGFLLVFIVPKF
                     QAIFRDMLGDKPLPAITQFVIGVSSIVKDHGLVLLGAIIVVLAAYKVIARTRAGRSTI
                     DRIQLRLPLFGDVLRKTAISRFSRTLGTLVTSGVPILQALNITRQTAGNTVIAHAISQ
                     VHDSVKEGESIVQPLDASGVFPPMVISMIDVGEETGQLPEMLLKIADVYDDEVDSSVA
                     GMTAALEPIMIVFLAVIVGTIVIALFMPLISIISGLQQQT"
     gene            complement(4047..5732)
                     /locus_tag="VFO30_08965"
     CDS             complement(4047..5732)
                     /locus_tag="VFO30_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9419458.1"
                     /translation="MNNKQVAELFIEQNVLQPSQADDVLSEADLNGKTIAQAMVDSGF
                     IDERGFYQTIAEGLGTEFVDLSETEIPEQILRLVPSGLARLHRALPIGIIGTTLRVVL
                     VDPLDVRAAEDLRFALGKDIDVVVAPSDQVEDRIKQYYGTETASMEEILKQLGEAGEL
                     LQVRETDGAEAVEAEANATPIIRFVDLILYQAILDRASDIHFEPFENEFKIRYRVDGA
                     LYEMSPPPRHLALPVISRVKVMANMNIAERRLPQDGRIQKNIAGRHVDLRVSTLPTQF
                     GESLVLRVLDRTTVNLDLAMLGMPDYIHDYLLELIHRPNGIFIATGPTGSGKTTTLYS
                     CLRKINTIDSKLLTAEEPVEYDLEGIVQVPVNEAIGLTFARILRAFLRQDPDRIMVGE
                     TRDLETAQIAIQASLTGHLVFTTLHTNDAPGAVTRLIDMGVEPFLISSTLEAVLGQRL
                     LRSICPECRTSYQPDQELLAELGIAPHEIGEKHFYYGKGCDACNDTGYKGRKGIYELL
                     KITDPLRELINERAPTVTIKQKAVELGMVTLRQDGLRSIFAGDTTVEEVLKYT"
     gene            complement(5770..7473)
                     /locus_tag="VFO30_08970"
     CDS             complement(5770..7473)
                     /locus_tag="VFO30_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9419459.1"
                     /translation="MARNENYVLDLLRDAGLVTKPQIERARTRLNGADNVVDRLVKDG
                     LLTEADVSRTLAAQAHMDWIDLSAIVIPPVVIKQIRAEDARRFKVIPVAFGETGLVVA
                     VGDPLDIDTIDSLNFLLQRDIEVVCTSPSKIRDALIKYYGTADEAADVLREKIGLDID
                     LGIEIGDGAALTETDEQDAPIIRMVSMLLIEAHRAGASDIHLEPLDKKFRVRFRIDGV
                     LQEMQAPPKRLQSAIISRIKIMTGSMSIAEKRLPQDGRIQVKIKKRPIDLRVSTIPTN
                     HGESVVMRVLDKSSLILGLPELGFFFDDQETFERLIRLPDGILLVTGPTGSGKTTTLY
                     ACLHYINKPDRKIITVEEPIEYQMTGINQVQVNPEIGMTFPAALRSILRQAPNVIMIG
                     EIRDPETANIATASSLTGHLVFSTLHTNDAPSAVARLIDIGVPPFLVASSVRAIMAQR
                     LVRRLCRNCKAPGELTETELRALRIDPGQIGDAQIMRPVGCEQCRRTGYRGRMGIFEI
                     FIIDDDVRHMINKRSSTLSLRQRARELGMRTLREDGVRKILAGVTSAEEVISITIGDV
                     S"
     gene            7659..8315
                     /locus_tag="VFO30_08975"
     CDS             7659..8315
                     /locus_tag="VFO30_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9419460.1"
                     /translation="MKRYLPFIIVGAVAALAIGAGAMLYQAKRTAMSSSAQSSATSKT
                     MKPEHVRGKMNAPIALEEFGDFQCPACATVAGMLRSLEAEYGSKLRVVFWNFPLAMHK
                     HGRDAALAVEAASLQGQFWQMHDLLYEKQSVWNTADDARPLFEQFATQLQLDVERFKK
                     DYASAEVAGRVDRQREYGVSRGVKNTPTIFVNGRELPPPFNPDRLREAMDGATPASKS
                     "
     gene            8643..>11624
                     /locus_tag="VFO30_08980"
     CDS             8643..>11624
                     /locus_tag="VFO30_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9419461.1"
                     /translation="MKRKVSARSAFFNPRLIAGFVLCFVGIALGLGAVSVTSGTADAQ
                     ATASDETTRSTVAQSDNGAAQRTKPQERTAVRSGRAFQGDLRTLPYIPVAKKERPERE
                     PPAFVPRVYQPPTGTSALAPNTGKSVLATAAVNAPAPTPSTTFDGLDYATWGAGHPPD
                     TNGDVGPTYYIQTVNTSIGIYNKSTGVRVAAFTFDTLMSQGQFGNLCDTDNFGDPVVL
                     YDSFENRWVITDFAFKIDNSGNVINPPGAFQCFAVSKTGDPVSGGWNFYSINTSGGLG
                     DYPKFGVWPDGIYMSANMFDYATTGSFQNVRVYAFNKAQMYAGAPTVQVVSFNVDPAD
                     FTLLPSNARLQSGTPPAGRPNLFISTWEYLNALTVYKFYVDWNHISLSTFTGPDVPAT
                     GSSWPNQSVPNAATPANTLDTLGIRAMAQNQYTNIAGTESLWTTHTVRRATNGFATPR
                     WYQVNVTGGTVAANTLQTASWDPNGADVTYRYIPSLAVDRAGDMALGYTTSNSTTNPG
                     LKYAGRLSTDAANTFSQTEQTLWQGTASQSGTCGASTCTRWGDYSAMTLDPDGCRFWY
                     TNEYYAVNGLNDLTRIGAFKYSQCTPVGSGGTVSGQVTIFGSGTPISGATVALGSRTT
                     TTNGSGNYSFSNIPAGTYPSITASAPGYNSATQSPVVVADNATATKNFALATAPVSAC
                     LTDTTQSDFQSGVPTNTDLNSSPGNVQLANSGSGFASSGNFISSLKDANPSSGSTAQW
                     TTLSWTGSAPASTTLRFQAAASNDANGVFDFVGPDGTPNTFFSSGGSLARFNGFRYLK
                     YKALLSTTNSSVTPVVNDVTVCFNNSPGGPTPTPTPTPTATPTPTPTITPTPTPIPKQ
                     DQTITFPPISTKTYGDANFDPGATASSGLAVTYTASGQCTIVSNKIHITAAGSCIVTA
                     NQSGNGQYNPARPVSQSFSILKAGTTTTSSLLANSPNPAPQFSDTAKLTATVKANTTV
                     TGNGVFSGTVSFTFNGVAVGSA"
BASE COUNT         2454 a         3271 c         3503 g         2396 t
ORIGIN      
        1 acattcggcc ggaactggtt cttccgaacc aaaccaggag cgtacgggag ttgctcgcgc
       61 agattactga tccggataaa gtgcttcggc tggtggagaa ctggtgatgc tgaacgtgac
      121 ggagaagttt cgcgcgatga aacagcgcgg cgagaagatc accgcgctga cggcttacga
      181 ttatccgacg gcgcggttgc tcgatgaaag tggaatcgac attgtgctag tgggcgattc
      241 gttaggcatg gtcgtgcttg gatatgaaga cacgacgcgc gtgacgttag aggagatgct
      301 gcaccacacg cgcgcggtcg cgcgcggggt gacgcgcgct ttacttgtcg cggacatgcc
      361 gatccacact tatgacaagc cggagcaggc cgtcgcgacg gcacagaagt tcgtcgaagc
      421 cggtgcgcaa tgcgtgaagc tggaaggtgg agcgagtcac ggcgcacaaa ttaaagcaat
      481 cagggcggcg ggcattccgt tcatggcgca catcgggatg ctgccacaaa gcgtccgcga
      541 agaagggggt tacaaagtaa aaggccgaac gcaatcggaa gccgaagcgc tgctcgccga
      601 tgcgcgcgca gtagaaagcg ccggcgcgtt ttcggtcgtg ctggagattg tgatcgccga
      661 tctggcgtgg caaatcagcc aggccatcag cattccgacg atcgggatcg gctcgggcgc
      721 gcactgcgac ggacaaattc tcgtaacgca cgatttgatc ggtttgttcc catggtttac
      781 gccgaagttc gtgtcgccgg aagcacgggt agcggacgaa attcgtaagg cagtacgcgc
      841 ctttatcgag cgaacgcgca gcgctcgacc ggtttgacgc gccaaagagg tggctcggcg
      901 aacgatgcta tttttcgcgc cggaggcgca cttttagcat ctcgtcggac atcgcgatcc
      961 accacgcatg tgatattgtt cagtgcatga gggtttcgaa cttcgatcag ttcgacgagc
     1021 tcgactacaa tcgcgcgttg ctgagcggca cgggcgagcg gaactggctc tggctcgggc
     1081 tgatcgggtc actcgcgctg cacctcgcgc tctgcgccta tttttaccgg gcgcgttttc
     1141 aatcagtcga gtcggtggtc gtgccgaacc agccgacgat cacgttcaaa gtgaagaatg
     1201 tcgatcttgc acagctggaa aagagcagct cggaccagac gagcgccgcg gccaagccgg
     1261 agccgaacag cccgactgac cagcagccgg acgagaagaa ggcgttcgac aagcttttgc
     1321 aggaggtgca ggcgagcacg gcaatgccgg acgacatcca aaatgttttg cctgataagc
     1381 ccaaagtgga acccgacatc acgtcggtgt tgccggacat cgagcgttcg accgcgcaga
     1441 cattatcaaa tagtccaaac gccacgcacg agcaatcggc gccgaacgac acggccgtct
     1501 ccggccggcc gcagccagcg ttgagtggaa ccgagcttgc gacgagtacg tcgattaaaa
     1561 ggccgagcac cttcacgagc aaattgccgg ccgacagtgc tggcccggag cgcggccgcg
     1621 cgggtttcag cgatctggat cagttgctcg cgcaaaaggg tcccctcggc tcggggacga
     1681 agttgcaact gcctgacgat cagctcttcg aatacgacag cgcggagctg cgaccgagcg
     1741 atttactctc gaaagtggcg acgctaatca ggcgcaatcc gaaagcgacg ttcacggtcg
     1801 agggctacac cgattcattc ggcacgccgg aatacaatct cgatctgagt caacgccgcg
     1861 ccgatagcgt gaagcagtat ttggtgcagg tgatgggaat tagtcccgat cagatcgaag
     1921 cgcgcggtta cggccagacg aaattccgcg tctcaccgaa cggctcaatc ggagagcagc
     1981 gcgaaaaccg tcgcgtcgaa atcgtcgtcc atacgaacga gccgtaacgg aattgcggat
     2041 tgcagatttc cgactgccga tttaccagtt tttgatccac tgcgtcggat ccgtcgaatt
     2101 gaccgtgctc caaagatcga atgtgggatt atatcctttt gtgctgtcgc ccgtggcagc
     2161 ttgaatcgtc gagtagccat agctgtttcc aaacgggtcg cgaatcgctg tgacatcggc
     2221 ggtcgacggt ggtgccggca acaacatgtt aggtttgaag ataaaataag ttctgctcga
     2281 cgaagtctct gaacgattcc cgctggttac gccggccagt tgttcataaa gatataaaga
     2341 agcggcggaa tacgccgacg ccgacgcgtc gaagtttgac cgcgcattca gagcattagt
     2401 gtctgaattt gatgggtaaa tcccgttatc ggctttgtag ctttcgcagg cagcggacat
     2461 ggcggcgatc tcggcttcgg tgcgggagcg cgcgcctttt ttctggacgt agccgacggt
     2521 ggcgaggacc agtgcggcca aaatgatgat gatcgtgatg acgaccagga gctcgaccag
     2581 cgtgaagccg ccgatgtggc gcgaatttcc aatttgcgct cctcgaattc gcaacttgaa
     2641 aagttgcgcc acaatcacgt ttgctgttgc aggccgctga tgatgctgat gagcggcatg
     2701 aagagcgcga tgacgatggt gccgacgatg acggcgagaa aaacaatcat gattggctcg
     2761 agcgcagcgg tcatgccggc cacagaagag tcgacttcgt cgtcgtagac gtcggcgatt
     2821 ttgaggagca tctccggcaa ctggccggtt tcttcgccga catcgatcat gctgatgacc
     2881 atcggcggga agacgccgct cgcgtcgagc ggttggacaa tggattcgcc ttccttgacg
     2941 ctgtcgtgca cctgtgaaat cgcgtgggcg atgacggtgt tgccagcggt ctggcgtgta
     3001 atgttgagcg cctgcagaat cggcacgccg ctggtgacga gcgtgccgag cgtgcgcgag
     3061 aaacgcgaga tggcggtctt gcgcagcacg tcgccaaaga gcggaagtcg cagctggatt
     3121 cgatcaatgg tcgatctccc ggcgcgggtg cgggcgatca ctttataagc ggcaagcact
     3181 acgatgatag cgccgagtag cacgaggccg tgatctttta cgatgctgct cacgccgatg
     3241 acaaattgcg tgatcgccgg cagcggcttg tcgccgagca tgtcgcggaa aatcgcctgg
     3301 aactttggaa cgatgaacac gagcaaaaag cccatgattc cgaccgccat ggtcataaca
     3361 atgatcgggt aaaccatggc agccgcgact ttgtttttga tcttctgcgc tttttcctgg
     3421 aactcggcga ggcggccaag gaccagctcg aggacgccgc cgagctcgcc ggcttttacc
     3481 atgttgacgt agagttcgtt aaaaatgctg ggatgttgcg ccaggctgtc ggaaaaggtg
     3541 ctcccgccct gaacgctgtt ggccaagctt tcaatcgcac ttttcaacgt cgcgtcgcgt
     3601 tcctggcgcg cgagcacgtt caagccgcgc agaagtggca gtccagaatc gattagcgtc
     3661 gccaattgcc gcgtgaaaat catcagcgtc ttggctttaa ctttcttgcg ccggaataga
     3721 gtgatctcca tgcgcaggcc ttttcctctg ggcgcagcga gcgacttgac tcttgttttg
     3781 cgcgccggct ttttcccatc gcgtccgccg gctttcgctt cttcgtagac gctggtcggg
     3841 aaaaatcccg cccgccgcaa ttggccgata gcttcgttac tcgaagacgc ttcgaccgaa
     3901 ccggtggatt cctgaccacg gccgtcgaga gctacgtaag cgaagcgagg catgactaga
     3961 ttaagcgaat taacgaatta gcgatttaca agccaaacgg ggaaatcagc gcgagggcca
     4021 ttatagtcgt tatactgctg atttttctac gtatatttga gcacttcctc gacggtcgtg
     4081 tcaccggcga agatgctgcg caagccgtcc tggcggagcg tgaccatgcc gagctcgacg
     4141 gctttttgtt tgatcgtcac tgttggggcg cgctcgttaa ttaactcgcg cagcggatcg
     4201 gtgattttga gcagctcgta aattcctttg cgacccttgt agccggtatc gttgcaggca
     4261 tcgcaaccct tgccgtagta aaaatgcttc tcgccgatct cgtgcggcgc gatgccgagc
     4321 tctgcgagca actcttgatc gggttgatac gatgtgcggc attccgggca gatgctgcgg
     4381 agcaagcgct ggccgagcac ggcctcgagc gtggatgaaa tcaaaaacgg ttccacgccc
     4441 atgtcgatca agcgggtgac ggcgccgggc gcgtcattcg tgtgcagcgt tgtgaagacg
     4501 aggtgaccgg taagcgacgc ctgaatggca atctgtgccg tctcgaggtc gcgcgtttcg
     4561 ccgaccatga tccgatccgg gtcctggcgc agaaaggcgc gtaagatacg cgcaaacgtg
     4621 aggccgattg cttcattgac tggcacttgg actatgcctt ccaagtcgta ctcgaccggt
     4681 tcctcggccg tcagcaattt agagtcgatc gtattgatct tgcgcaggca ggaataaagc
     4741 gttgtcgttt tgcccgagcc ggtcgggccg gtcgcgataa agattccatt cggccgatga
     4801 atcagctcga gcagataatc gtggatgtaa tcgggcatgc cgagcatcgc gaggtcgaga
     4861 ttgactgttg tgcggtcgag gacacgcaaa accaagcttt cgccaaactg cgtcggcaac
     4921 gtcgagacgc gaagatcgac atgtcggccg gcgatatttt tttgaatgcg tccatcctgc
     4981 gggagccggc gctcggcaat gttcatgttc gccattactt tgacgcgcga gatgaccggc
     5041 agggcgaggt gtcgcggcgg cggcgacatt tcgtaaagcg cgccgtcgac acggtagcgg
     5101 atcttgaatt cgttctcgaa gggctcgaag tgaatgtccg acgcgcggtc tagaatcgcc
     5161 tggtagagga taagatcgac aaaacgaatg atcggcgtcg cgttcgcttc cgcttcgaca
     5221 gcctcggcac catctgtttc gcgcacttgg agaagctcgc ccgcctcgcc gagctgcttg
     5281 aggatctcct ccatgctcgc cgtctcggtg ccgtagtatt gtttgatgcg gtcttcgacc
     5341 tggtctgatg gagcgacgac aacatcgata tctttgccga gggcgaagcg gagatcctcg
     5401 gccgcgcgta catcgagagg gtcgacaagg acaacgcgca acgtggtgcc gatgattcca
     5461 attggaagcg cgcggtgcaa ccgggccaag ccacttggaa caaggcgcag aatttgttct
     5521 ggaatttcgg tctcgctaag atcgacaaat tcggtgccga ggccttccgc gatcgtttgg
     5581 tagaaaccgc gttcgtcgat aaaaccgctg tcgaccatcg cttgtgcgat ggttttgccg
     5641 ttcaagtccg cttcgctcag cacgtcatcg gcctgtgagg gctgaaggac gttctgttcg
     5701 atgaatagct cggcgacttg tttgttgttc attatttcgg tgacgggtta aagcgttgaa
     5761 gagttatagc tagctgacgt ctccaatcgt aatggagatc acttcctcgg cgctggtgac
     5821 gccggcgaga attttgcgga cgccatcttc gcgcagcgtg cgcatgccga gctcacgggc
     5881 gcgctgccgc agcgacaaag tcgagctgcg cttattgatc atgtggcgca cgtcatcgtc
     5941 gattatgaaa atctcgaaga tgcccatccg gcctctataa ccggtgcggc ggcattgttc
     6001 gcagccgacc gggcgcatga tttgcgcatc gccgatttgg ccggggtcga tccgcagcgc
     6061 gcgcagctcg gtttcagtga gttcaccggg cgctttgcag ttccggcaaa gccggcggac
     6121 gagccgttgc gccatgatcg cgcgcacgga agaggcgacc agaaagggcg gcacgccgat
     6181 gtcgatcaag cgcgcgacgg ccgagggcgc gtcgttcgta tgcagtgtac tgaagacaag
     6241 gtgtccggtg aggctcgacg ccgtcgcgat attggccgtc tccgggtcgc ggatttcgcc
     6301 gatcatgatg acattgggtg cctggcgcag aatcgagcgc aaagcggctg gaaacgtcat
     6361 gccgatttca ggattcacct gcacctgatt gatgccggtc atctggtact cgatcggctc
     6421 ctcgaccgta atgattttgc gatccggttt attgatgtag tgcaggcagg cgtaaagggt
     6481 ggtcgttttg ccggagccgg tcggcccggt gacgagcaga attccatcgg gaagtctgat
     6541 gagccgctcg aatgtttcct ggtcatcgaa gaagaatccg agctcgggca atccaaggat
     6601 aaggctcgat ttgtcgagca cgcgcatgac gacgctttcg ccgtgattgg tcgggatggt
     6661 tgagacgcgc aggtcgatcg gtcgcttttt gattttgacc tgaatgcgcc cgtcctgtgg
     6721 aagacgtttc tccgcgatgc tcatcgagcc ggtcatgatt ttgatacgac tgatgattgc
     6781 ggattgcagg cgtttcggcg gtgcctgcat ctcctggagg acgccgtcaa tgcggaaacg
     6841 gacgcggaat tttttgtcga gcggttcaag gtgaatgtcg ctcgcgccag cgcgatgtgc
     6901 ctcgatgagt aacatcgata ccatgcgaat gatgggcgcg tcctgttcat ccgtctcagt
     6961 gagggcggcg ccgtcgccga tctcaatgcc aagatcgata tcgagtccga ttttttcccg
     7021 taggacgtcg gcagcttcat ccgcagtgcc gtaatattta atgagcgcat cgcggatttt
     7081 actgggactg gtgcaaacga cttcgatgtc gcgctgcaag aggaagttca ggctatcaat
     7141 cgtgtcgatg tcgagcgggt cgcccaccgc gacgacgagc ccagtttccc caaaggcgac
     7201 cggaatcact ttgaagcggc gcgcgtcttc ggcccggatt tgtttgatga cgaccggcgg
     7261 gatgacgatc gccgagaggt ctatccaatc catgtgggct tgggcggcga gggtgcgcga
     7321 cacgtccgct tccgtgagaa ggccgtcttt gaccaggcga tcgacaacat tgtcggcgcc
     7381 attcagacgc gtccgggcgc gctcgatttg cggcttggtg acaagcccag cgtcacggag
     7441 cagatcaagg acgtagtttt cgttccgagc catggcgaaa gcgggacgcc aaatttaccg
     7501 caaaaactgg gctaacaggg cgaaaaagtg caaaaagttt gacactcggc agctggggcg
     7561 tatacacgct gccgctcagg ttttctccgt gctcgctgcg cgatgcagcg tccggattta
     7621 acttgtgcgg cgacccaggc ccgggggcga actccaacat gaagcgatat cttcctttta
     7681 taattgtcgg cgcggtggcg gcgctggcca tcggcgccgg agcgatgctg tatcaagcga
     7741 aacgaacggc gatgagcagc agcgcgcagt caagcgctac atccaagaca atgaagccgg
     7801 agcatgtgcg cggtaaaatg aatgcgccga tcgcgctgga agagttcggt gatttccaat
     7861 gtccagcctg cgcgacagtc gcgggcatgc ttcgttcgct ggaggcggag tatggatcga
     7921 agttgcgcgt tgtattttgg aatttcccgc tcgccatgca caagcatggc cgtgacgcgg
     7981 ctttggccgt cgaagcggca tcactccagg gacaattttg gcaaatgcac gatttgcttt
     8041 acgagaaaca gtccgtctgg aacacagcgg atgacgcccg tcccttattc gagcaatttg
     8101 cgacgcaatt gcagctggat gtcgagcgtt tcaaaaagga ctatgccagc gcggaagtcg
     8161 ccgggcgcgt ggatcgtcag cgcgagtacg gtgtgtcccg cggcgtaaag aatacgccca
     8221 caatttttgt gaacggccgc gagctccctc cgccgttcaa cccggaccgc ttgcgcgagg
     8281 caatggatgg cgcgacgccg gcgtcgaaat cctgattgtg ggaaagcaga gaatcttcgg
     8341 ataaaaaagc gcgtggacga cgcgccttct gccgctcctg gctgctctca gcggcgctca
     8401 cttttgctca gctggcttgg tgctggcgac tctccgccgg gtggctagtt gtcgatcttg
     8461 tcagcaccgg cacgtgcagc agaacgaaga gctcggggag acggaagttt gtaaaaggca
     8521 ttgacggaat cgtcgtgcgc ggtactcacc gtgctgcatt tagcgattga ggtccgcgaa
     8581 ccgtcatcgc cagctcacgt cgaagatccg cgtccttaaa actggatgga ccagccccct
     8641 gcatgaaaag aaaagtttcg gcgcgatcag cgttttttaa cccccgcctt atagcgggtt
     8701 tcgtcctctg ctttgttggg atcgcccttg gattgggcgc ggttagtgta acgagtggga
     8761 ccgcggacgc gcaggcgacg gcgtcggatg agaccactcg cagcacagta gcgcagagcg
     8821 acaatggcgc ggcacaacga acgaagccac aggagcgaac tgccgtccgc tcgggtcgcg
     8881 ctttccaagg cgacctgcgg acgctgccgt acattccggt ggcaaaaaag gagcgcccgg
     8941 aacgcgaacc gccggcgttc gtcccacgcg tttatcaacc gcccactgga acttctgccc
     9001 tcgctcccaa cacggggaaa tcggtgctag ctaccgctgc ggtcaacgcg cccgcgccca
     9061 cgccgagtac gacattcgac ggactggatt acgcgacctg gggcgccggt cacccgcctg
     9121 acacaaacgg ggatgtcggg ccgacctatt acatccagac agtcaacacc tcgatcggca
     9181 tttacaacaa gtcgacgggt gtgcgcgtgg ccgcgttcac cttcgacacg cttatgagcc
     9241 aggggcaatt cgggaacttg tgcgacacgg ataatttcgg cgacccggtc gtgctctacg
     9301 acagcttcga gaaccgctgg gtgatcaccg acttcgcctt caagatcgac aactcgggca
     9361 atgtgattaa tccgcccggc gcatttcaat gcttcgccgt ttcgaagacg ggcgatccgg
     9421 tgagtggcgg ttggaatttt tattccatca acacaagcgg cgggctggga gattacccaa
     9481 agttcggagt gtggccggac ggaatttaca tgtcggccaa catgttcgat tacgcgacca
     9541 ctggctcgtt tcagaacgtg cgcgtctatg ccttcaacaa agcgcagatg tatgccggcg
     9601 cgccaactgt gcaggtagtc agctttaatg tcgatccagc cgatttcact cttttgccca
     9661 gcaacgcacg cttgcagagc ggcacgccgc ccgcgggcag gccgaacctc ttcatctcga
     9721 cctgggaata cctgaacgcg ctcacggtat ataaatttta cgtcgactgg aaccacatca
     9781 gcctttcgac tttcaccggg ccggatgtgc ccgcgaccgg ctcgagttgg cctaaccagt
     9841 cagtgccgaa tgcagcgacg ccggcgaaca ctctcgacac tcttggtatc cgcgcgatgg
     9901 cgcaaaatca gtacaccaac atcgccggga ccgagtcgct ttggacgact cacaccgtgc
     9961 ggcgagcaac gaacggtttc gccacgccgc gttggtatca ggtgaacgtg actggcggca
    10021 cagtcgcagc caacaccttg caaaccgcga gctgggaccc gaacggcgcg gacgtgactt
    10081 accgctacat cccgagcctg gcagtcgatc gtgccggcga tatggcgctg ggctatacga
    10141 cttcgaattc gacaaccaat ccgggattga aatacgccgg ccggctctcg accgacgcgg
    10201 cgaacacatt cagccaaacc gaacagacac tctggcaagg cactgccagc cagagtggca
    10261 cttgcggcgc ttcgacgtgc acgcgctggg gcgattacag cgcgatgacg ctcgacccgg
    10321 acggctgcag gttttggtac acgaacgaat attacgctgt caatggactg aacgacctca
    10381 cccgcatcgg ggcgttcaag tactcgcagt gcacgcccgt cggcagcggg ggcaccgtct
    10441 caggccaggt gaccattttc ggcagcggca caccgatcag tggcgcgacg gttgcgcttg
    10501 gcagccgcac gacaacgaca aacggcagcg gcaattactc atttagcaac attccggccg
    10561 gcacgtatcc cagcatcaca gcgagtgctc ccggctacaa ctccgcgacg caatcgccgg
    10621 tggtggtggc cgacaatgcg acagcgacga aaaactttgc gctcgcgact gcgccagtga
    10681 gcgcgtgtct caccgataca acgcaatcgg atttccaaag cggcgtgccg acaaataccg
    10741 atttgaacag cagcccgggc aatgtgcagc tcgcgaattc gggcagcggc tttgcgtcgt
    10801 caggcaattt tatctcctcg ctgaaagacg cgaacccttc cagcggctcc accgctcaat
    10861 ggacgaccct ttcctggacg ggctcggcgc cagcatcaac gaccttgcga tttcaagcgg
    10921 cggcgagcaa cgatgctaac ggcgtgttcg acttcgttgg ccccgatggt acgccgaaca
    10981 catttttcag cagcggcggc tcgctcgcgc gcttcaacgg tttccggtat ctcaagtaca
    11041 aagcattatt gagcacgacc aacagttcgg tcactccggt cgtgaacgac gtcacggtat
    11101 gttttaataa ctcaccgggt gggccgacac cgactccaac acccactccc acagctacac
    11161 caactcctac tcccacaata accccgacac ctactccaat tcccaagcag gaccagacga
    11221 ttacgttccc gccaatttcc accaagacat acggcgacgc taactttgac cccggtgcaa
    11281 cggctagctc gggcttagcg gtgacctata ccgcctcggg ccagtgcacg attgtttcga
    11341 acaagatcca cattactgcc gccggctcat gcattgtgac ggcgaatcag tcgggcaacg
    11401 gtcagtacaa tccagccagg cccgtgtcac agtcgttcag catcctcaag gcaggcacaa
    11461 ccaccacgtc ttcgttgctg gctaattcgc cgaatcccgc gccgcagttc agcgatacgg
    11521 ccaagcttac cgccacagtc aaggctaata ccactgtcac cggcaacggc gtgttttccg
    11581 gaacagttag ctttaccttc aacggtgtcg ctgttggtag cgcg
//
LOCUS       DASTDG010000269        11850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_830841, whole genome shotgun sequence.
ACCESSION   DASTDG010000269 DASTDG010000000
VERSION     DASTDG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830841"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFO30_08985"
     CDS             <1..228
                     /locus_tag="VFO30_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419462.1"
                     /translation="LVEEALNQAQEIMYEDVPARVLRSEHLVAICLQTGRRKDRERVR
                     ILRKQANLDMNFLASVLRRHQLEEKWKLWTE"
     gene            528..1631
                     /locus_tag="VFO30_08990"
     CDS             528..1631
                     /locus_tag="VFO30_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HET9419463.1"
                     /translation="MKLKLPSVALAALLFAGLPLASSSAYIGISVGIAPPPIPIYEQP
                     YCPVEGYIWTPGYWAYDGFGYYWVPGAWVPPPRIGFLWTPGYWGYDGGRYFFNEGYWG
                     PTVGFYGGINYGYGYGGHGYYGGEWVGNAFRYNTAVTRVDTRVIRNTYVNRNVVTTTS
                     TRASFNGPGGVTARATAQEQAAAKAERIPATAAQQSRVEAAKKDPALHVANNHGKPKA
                     EAVKAFDRNSGTQAVAGKAKGGAAPEAQGNSAFGQKQGNAATRTTGSQNNAYGKAQSA
                     EARSRAAAKAARTNEAAAPRAPKAERMKPNSHANNAAIAPRAPQGAQRVSNRPQPASN
                     RRAAPAVNERAKGQNAQPDKKKKKSGNEAPGRG"
     gene            complement(1838..2215)
                     /locus_tag="VFO30_08995"
     CDS             complement(1838..2215)
                     /locus_tag="VFO30_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419464.1"
                     /translation="MKTYLRYILAGVSFAALCGCANMPGMGAKSVAGTWTNSLGTVWA
                     IKPDNTFEVDRYGKGKTDIRGTYAIKGDTFTITGNEGKIPKGCEGQGVYKFSRKGNDL
                     TFTLVSDTCKDRKKNVLSPWHRQ"
     gene            complement(2446..3018)
                     /locus_tag="VFO30_09000"
     CDS             complement(2446..3018)
                     /locus_tag="VFO30_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419465.1"
                     /translation="MSVLGLQSILATFMKDSVVRGRLLQLLYERRNEGFIPFGHAEQA
                     TPPPGGINRRDWMRAVAQLSEYRLIDWTPLKDESGMGVLSGFAKINDSGVKVLEAGVA
                     PPISISIDESRRTTVPRQQQVPIATSTPQQQVITDALEKVITAINQANVPETEKNEAI
                     SLLRELLASKAAASALGAGAQSLTAKYFTG"
     gene            complement(3171..3914)
                     /locus_tag="VFO30_09005"
     CDS             complement(3171..3914)
                     /locus_tag="VFO30_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419466.1"
                     /translation="MTKLSFATAAIAVICSVSLSRGQESPAATGSPAASAPAPRPEVY
                     HVIFVHAATGKASALEDAMKKAAASGPMPGHNLGLRHEAGAPWDYVGIQHLGTKATVE
                     ATGNPQGATMRPLMDWHDDTFVNGPAWPDFAKAMGLDESGKPKSNEDVYVVSVYRPIP
                     GQDDALDKFLAEPPSASGDLAVGTVVLQHLEGGAWRFLSISHYKNFQDYAASEVKSVA
                     ETAKGTGGWYRLRELCSFHNDTITVHTKP"
     gene            4213..4440
                     /locus_tag="VFO30_09010"
     CDS             4213..4440
                     /locus_tag="VFO30_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419467.1"
                     /translation="MTKTVSKIGNSQGIIFDTALMDLARIKVGDKLTVTVHEGGSIVL
                     TPVNPTLDPAKAAATAKRLIHKNARLFKRLA"
     gene            4437..4847
                     /locus_tag="VFO30_09015"
     CDS             4437..4847
                     /locus_tag="VFO30_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET9419468.1"
                     /translation="MSKSLAHLTVAAVKAIHAEVLAAHGGSPGIRDANLLESAVAAPQ
                     ATMMGQPLIFDPVEIAAAYLFYICRNHPFVDGNKRTALASCLVFLKANGLLRSEKLQM
                     HEWEKFVVDVAASRLDRDQTTARLRKLLKRKSKG"
     gene            4888..5394
                     /locus_tag="VFO30_09020"
     CDS             4888..5394
                     /locus_tag="VFO30_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419469.1"
                     /translation="MEPAEALGVAAQVAVTLAGFAGVVVVFRPQAVHQWSTLDRFRLR
                     LLLSSSIFPLGYALFAMLLLTMIPPPPQIWRWCSALAAIGSVAGGITTLIAMRKISQA
                     ELEGTTRTLFYPVAVIGTAAILLQFYNFSVLNRFWPFFFAIVVHIIAAMLQFMRIVLL
                     PPDTIVPK"
     gene            complement(5675..5848)
                     /locus_tag="VFO30_09025"
     CDS             complement(5675..5848)
                     /locus_tag="VFO30_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419470.1"
                     /translation="MHQIKFQQIVRDAEVANAILSVVRRRHLTEADIEKALTELHVPR
                     AAYIARISVLLGL"
     gene            complement(5906..6157)
                     /locus_tag="VFO30_09030"
     CDS             complement(5906..6157)
                     /locus_tag="VFO30_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419471.1"
                     /translation="MSDQNGNSRLFRRRRAKLIRFIIAETSALGILLLAGTFVLSSRL
                     VDSALVAAMNIVTIVAAAAVATIPIIFFAITPILPRAGR"
     gene            6545..7198
                     /locus_tag="VFO30_09035"
     CDS             6545..7198
                     /locus_tag="VFO30_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9419472.1"
                     /translation="MQKRDSAKTQRARLLVLVDESNVGGSVRTAGRGLDWLKLRDFLA
                     GPRTGRDLIEMVVYAGLPPAIPAWQEERDKKNKFVQWLRSNGFMVVTKDGSPAEEGHY
                     KANVDVMMAIDAMELSIAMRPDVVILVTGDADFAYLATQLRRHGIRVEVASVAAILGH
                     TLRAAANEVIDLAPLFRTFEMMKIAEPTPARALPPPPQQQQQRRRRPRFRSGQGTTT"
     gene            7670..7990
                     /locus_tag="VFO30_09040"
     CDS             7670..7990
                     /locus_tag="VFO30_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419473.1"
                     /translation="MTKLIALVTAGVFAASVAFAGEHGACTKQAGNKENMVACQVSLA
                     NLNLTPEQKSKMDSLMEEHHKEGCTAESEAKYMKEAKKILTKEQYATFKAECKKGEKE
                     KTEA"
     gene            8154..8987
                     /locus_tag="VFO30_09045"
     CDS             8154..8987
                     /locus_tag="VFO30_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9419474.1"
                     /translation="MEMSANTSGDYVLGTHEEELARLGLQHRVWRPVVLECWQRAGIT
                     VGSRVLDVGAGPGYGTVDLAEIIGPTGHVDAVERSTNFVNAIRATCRARALSNVQVHE
                     LDLMVEDLPSGPYDFTWCRWVLCFVSNPDLLVRRIAAVTRTGGRAIFHEYGHYTTWRF
                     FPHRPALEEFVGHVVATWRQSGGEPDTGLMLPEWLNENGFSVLSVEPRIFCVRPNDYM
                     WQWPAQFIDVHLGRLQELGRINKSFAAKVRAELAAAEKGDASFLITPLVLEIIAERVR
                     K"
     gene            9083..10462
                     /locus_tag="VFO30_09050"
     CDS             9083..10462
                     /locus_tag="VFO30_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9419475.1"
                     /translation="MKDLTQGSVTKHLLHMSAFMAVSMLAQTLYLLADLFWVGRLGKE
                     AIAAVGVAGNLMMIVLALTQMLGVGTTALISQAAGRKDQTHAEVVFNQACMMSILIAA
                     ALGLLGFLTMDIYANSLSADGTTARLAKAYLWWFLPALLLQFPLVAMSSALRATGIVR
                     APVMFQVLTVVLNMVLAPFLIFGIGPWPRLGVSGAALATFISILVGNVLIIFYFEKKY
                     RYLRFRFPLFRPQAKIWSAMLRIGVPAGAEFILLFFYIVLVYAIIRGFGPAAQAGFGV
                     GARVMQALFLPVVALSFAVSPVVGQNFGGRRADRVRQSVYSGLGIATVLMVVLALITW
                     AAPEVMIRFFSKDARVIAFGSDYLRIVSFNFVAAGIVFTSSSMFQGLGNTVPPLLSSA
                     TRLILFALPAVLISRASGFDIRHVWYLSVGSQVLQACINLVLLRRELHHKLRFAEGEG
                     FIPASATAS"
     gene            10583..11116
                     /locus_tag="VFO30_09055"
     CDS             10583..11116
                     /locus_tag="VFO30_09055"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9419476.1"
                     /translation="MKLKVTVLLSLLFILLPSVAVRAQTKSSASAKPVRETAAESDIR
                     KWLDDWTKAFARKDVDAVIALYADDVIAYDVVPPLQYVGKEAYRADYQQFFGQYQSDL
                     HVEVRDLHIGVRGDFAYAAGLELIGGTLKNGQKSDVWVRFTSLFRKVKGRWLDFHDHV
                     SVPADFDTGKALLDLKP"
     gene            11167..>11850
                     /locus_tag="VFO30_09060"
     CDS             11167..>11850
                     /locus_tag="VFO30_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9419477.1"
                     /translation="MHDLKLITWPLGCRVMMRLVFICAALSIFGGAAFGAGEYQRTKD
                     RKTLIWNADPKSDDSASWWGERDKDGYAKGVGELTWYTAQGTVYARYYGNMVHGKFDG
                     PVNVHVKGRTAHAFFSGGKRMTSWARGPASSRFATDWRSALAREKAEAAPATSPPPTS
                     TEPVSASEELRGSAQQTQESPAEGPTGEAASKKEDLAKAKFAPAELPARGEEGATKAE
                     ENVQPPTPNP"
BASE COUNT         2544 a         3221 c         3671 g         2414 t
ORIGIN      
        1 ttagtcgagg aagcgctcaa ccaggcgcaa gaaattatgt atgaagatgt gccggctcgc
       61 gtgctgcgaa gcgaacatct ggttgccatt tgtctgcaaa ccggacgcag gaaggatcgg
      121 gagcgcgtcc ggattttgcg caaacaggca aacctagaca tgaacttcct tgccagcgtc
      181 ctgaggcggc atcagcttga agagaaatgg aagctttgga cggagtaagc gcggaggtag
      241 cgaaaatatt tgccgcgaag gaacaacgcc ggcagagact ggcgcgcctg ccatttccag
      301 agaaagtgcg ggccgtgatt caactacaag agatgatcgc cgtgattcta cgcgctcgcg
      361 gcaaatttgt gcagccgtgg caggtcgcga caagcggttc aacgccggca aagcctggct
      421 gttgaaccca ttaactggcg ttgggatggc cgccccatga agtcggtcag ggtttcgtct
      481 gaatagtttg cgacgtaata acgtctcttg tgttaataca tctgaatatg aaactgaaac
      541 tcccctctgt tgcgctcgcg gcactgctct ttgccgggct accgctggct tcttcttcgg
      601 catacatcgg catatcggtt gggatcgcgc cgccgccgat accaatttac gagcagccgt
      661 actgcccagt cgaaggctat atctggaccc ccggctactg ggcgtatgat ggtttcggtt
      721 attactgggt gcctggcgcc tgggtccctc caccgcgcat cggatttctg tggacccctg
      781 gatactgggg ctacgacggc ggccgttact tctttaacga aggctactgg ggcccgaccg
      841 tcgggttcta cggcgggatc aattacggct atggctacgg aggccacggc tactacggtg
      901 gcgaatgggt tggtaatgcg ttccgctata acacggccgt gacacgagtg gacacgcgcg
      961 tgattcgcaa cacctacgtc aacagaaacg tcgtcaccac gaccagcacc cgcgccagtt
     1021 ttaacggccc gggcggcgtc accgcgaggg ccactgcgca ggaacaagcc gccgcgaagg
     1081 cggagcgcat cccggcaacc gcggcacagc agtcacgcgt cgaagcggcg aaaaaggatc
     1141 ccgctttgca tgttgctaac aatcacggca agcccaaggc cgaagcagtc aaggcattcg
     1201 accgcaacag cggcacgcaa gctgtcgccg gcaaggcgaa gggcggagcc gctccggaag
     1261 ctcagggtaa ctccgcattc ggccagaaac agggcaatgc agccacccgg acgacgggct
     1321 cacaaaacaa cgcctacggc aaggcgcaat cggctgaagc acgttcgcgc gcggcagcga
     1381 aagctgctcg gactaacgaa gcagcggcac cgcgcgcccc taaagcagag cggatgaaac
     1441 ccaatagcca cgccaacaat gcggcgattg ctccgcgcgc tccgcagggt gcgcaaagag
     1501 tgtccaatcg accgcagcca gcgtcgaacc ggcgagcggc acccgcagta aatgagcggg
     1561 ccaaagggca gaacgcgcag ccggacaaaa agaagaaaaa gtccgggaac gaagcgccag
     1621 gccgcggtta gtagcaggcg gcgcgcaacg cgcgcgatgc tttagcgcag atccggcagc
     1681 tggtgtgttt tgctgtgtcg cgcccgcgcg tacggccgag cgcgagcggc ggcggtattg
     1741 ggactgcgtg cttgcgcgat ccgctcgcac cgtggcgatc accgctcaca gatcagtgat
     1801 ccactaatca ctaatcacca gtcaccgatc actaaaacta ctgccggtgc caggggctaa
     1861 ggacattttt cttacggtct ttgcaggtgt cgctgacgag agtaaaggtg aggtcgttgc
     1921 ctttgcggct gaacttgtaa acgccctggc cttcgcagcc cttgggaatt ttgccttcat
     1981 tgcccgtgat cgtgaaggtg tcgcccttaa tggcatacgt gccgcggata tcggttttgc
     2041 ccttgccgta ccggtcgacc tcgaatgtgt tatcgggctt gattgcccag acggtgccga
     2101 gcgagttggt ccaggtgccg gccacagatt tggcacccat accgggcatg ttcgcgcacc
     2161 cgcatagcgc ggcgaaagag acaccagcca aaatgtaacg cagatatgtt ttcatggcgc
     2221 gatgttcttc gatgccgcgc gaaggtcaag ccggatcgac atacggaagt aaagcgtctc
     2281 gctttacttg gacgcgatgc gtctcgcttc gcgcgggaga ttatgttgag cggggacgct
     2341 caactgccga gtccggcgag gcgccggact tccgaatcga atgctcaaat ttgggcgtga
     2401 acaatcgaca aataccggtt cgccccatcc agcggctagc ttgctctatc ccgtgaaata
     2461 tttcgcggtg agggattgcg cgccagcgcc tagcgcactg gccgctgctt tgcttgccag
     2521 cagttctcgc aacaaagata tcgcttcgtt tttctccgtc tccggcacat tggcttgatt
     2581 gatcgcagtg atcactttct ccagtgcgtc cgtgatcacc tgctgctgcg gagtgctcgt
     2641 tgcgatcggc acttgttgct gccggggaac ggtggtccgt cggctctcat cgatggagat
     2701 cgaaatcggg ggcgcgacgc cagcttcgag cactttgact ccggagtcgt ttatcttagc
     2761 aaagccggac aacacaccca tcccgctctc atctttaagc ggggtccagt cgatgaggcg
     2821 atattctgaa agctgggcca cggcgcgcat ccagtcgcgg cgatttatac ctcctggcgg
     2881 cggcgtcgcc tgctcagcat ggccgaacgg aatgaaacct tcgtttcgac gttcgtagag
     2941 aagctgcagc aggcgtcctc tgacgacaga gtccttcata aacgttgcca atatgctctg
     3001 caggccgagc accgacaact catcttgtcg gatacagaat gggatcgcgt ctaaactccg
     3061 aaagcattcg cgagccaaat cgcgacgcga ttccagtgat tgacttttat cgacaccaac
     3121 gcacgctgcg acgctttcac gtatctaaga tcgacaatgt ggcggcgcgg ttacggcttg
     3181 gtgtgcaccg taatggtgtc gttatgaaac gagcagagct cgcgcagccg ataccagccg
     3241 ccggtgccct tggctgtttc ggcgacgctt tttacttcgc tcgccgcgta gtcctggaag
     3301 tttttgtagt gcgagatgga aaggaaacgc cacgcgccgc cttccaaatg ctgtagcacc
     3361 accgtgccga ctgcgagatc gcctgaagct gacggcggtt cagcgaggaa tttgtcaagc
     3421 gcgtcgtctt gcccggggat cggacgataa accgagacga cgtaaacgtc ttcgttgctc
     3481 ttgggttttc ccgattcgtc gagccccatg gccttggcga aatcaggcca ggccgggccg
     3541 ttgacgaagg tgtcgtcgtg ccaatccatc agtggccgca tcgtcgcgcc ctgcgggttg
     3601 ccggtcgctt cgaccgtagc cttggtgccg aggtgttgaa tgccgacgta atcccacggc
     3661 gcaccggctt cgtggcgcaa accgagattg tggccgggca tgggaccgct ggcggctgct
     3721 ttcttcattg cgtcttccag cgcgctcgct ttgcccgtgg ccgcgtgcac gaaaatcacg
     3781 tggtaaacct caggccgcgg cgcgggtgcg cttgccgccg gcgaaccggt ggctgccggc
     3841 gattcttggc cacggctgag actgaccgaa cagatgaccg cgatggctgc ggtcgcgaat
     3901 gataactttg tcatcggact cctttatgcg ttgagcccgt tcgaccccca tcgccctcgc
     3961 cgaacgtgcg aatgttttga ttgtgtgcgg atgggggccc gcgcgcgcca atcgggatag
     4021 cgacctggct gtgggcagtc aatgcttttc caagcgtaaa atcctgagtc gccgcgatga
     4081 tttaatcaga accgcagcac gaataaccga tgatacgccg acagggtcta tcgacagcga
     4141 cgaggctatt gaacaggccg gccgggaaat cgtttacttg caaacagttt ttgtaaaaca
     4201 tagtattacg ccatgaccaa gacggtttcc aagatcggca actcgcaggg gatcattttt
     4261 gacacggcgc tgatggattt ggcccggatc aaagtgggcg acaaattaac tgtaaccgtg
     4321 cacgagggcg gctcgatcgt tctgactcca gtgaacccga ctttggatcc ggccaaggcc
     4381 gccgctacgg ccaagcgatt gatccacaaa aacgcgcggt tgtttaaacg gctcgcgtga
     4441 gcaaatcgct cgcgcatctg acggtcgcgg cggtcaaggc gatccacgcg gaagtgctcg
     4501 ccgcgcatgg cggttcgccc ggtatccgcg acgcaaacct tctcgaatcg gcagtcgctg
     4561 ccccgcaagc gaccatgatg ggccagccgc tgatcttcga tccagtcgag atagcggcgg
     4621 cttacctatt ttacatctgc cgaaatcatc cgttcgtcga tggaaacaag cgcacagcgc
     4681 tggcgtcgtg cttggtcttc ctcaaggcga acggattatt gcgatcggaa aaattgcaga
     4741 tgcatgagtg ggaaaaattt gtggtcgatg tggcggcgag ccggctggat cgcgaccaaa
     4801 ccactgcccg tctgcgcaaa ttgctgaaga gaaagtctaa aggttaaaag tggaacgcag
     4861 ggatcgcttt tggttaactg accgataatg gaaccggcgg aagctctcgg cgtcgcggcg
     4921 caggtagcag tgaccctcgc cggctttgcc ggtgtagtgg tggtttttcg gccgcaggca
     4981 gtgcatcaat ggtcgactct cgaccggttc cgcctccgat tgctcctgag cagttcgatc
     5041 ttcccgctcg gctacgcatt gtttgcgatg ttgctgctca cgatgatacc gccaccgcca
     5101 caaatctggc gttggtgcag cgcgctcgcg gccattggtt ccgttgcggg cggaatcacg
     5161 actctcattg ccatgcgcaa aattagccag gccgaactcg aaggcactac gagaaccctg
     5221 ttctaccccg tcgctgtcat cggaacggcg gcgattttac tgcaattcta caacttctcg
     5281 gtgctaaatc ggttttggcc gttcttcttc gccattgtcg tacacatcat tgccgcgatg
     5341 ttgcagttca tgcgcatcgt ccttctgccg ccggacacga tcgttccaaa gtagaaattg
     5401 gaagaagaca aaggcaattg aagtcgaccg aacgtcaatc tgtagcggga acaaaacgcg
     5461 aaacaacttg gaaccggcta cgaacagatc actattaacg caatgggaaa cagtaaaggc
     5521 agagataacg ccaggaaacg cgcgcgccgc cggaagaaga cggagcggct cgccctcgcc
     5581 aagcgcaaac agaaggcggc gacggcgaag acgccgggtt aatcattatt gattatagga
     5641 tgcgaactgc gaaatgcaga acgccgacgg tgagttaaag gcctaacaag acgctgatcc
     5701 gggcgatgta ggccgcgcgc ggaacatgca gctcggtcaa cgctttctcg atatctgcct
     5761 cggtgagatg gcgtctgcgc accaccgaaa gaatggcatt ggcgacctct gcgtcgcgaa
     5821 cgatttgctg aaacttaatt tgatgcattt gacaaggggc taacttccac gggacgaggc
     5881 gagatcgaag atggcattgg taaggctagc gtcccgcgcg gggaagaatg ggcgtgattg
     5941 cgaaaaagat gatcgggatc gtggcgacgg ccgcggcggc aacgatcgtc acaatattca
     6001 tggctgcgac caaggcggaa tcaaccaggc gcgatgacaa aacgaacgtt ccggccagga
     6061 gaagaatgcc aagtgctgaa gtctcggcga tgataaatcg gataagcttg gcgcggcgtc
     6121 gccggaacaa ccgcgagttc ccgttttgat ctgacatgtc gatgttaaag agcgcgttat
     6181 cggcgttagc ggccattgtc ggggtgaaag cgccgtgaga cgtgtgagtc ggcgaaggtc
     6241 cttcggcgaa cgtcgggcag ctgaattctc gactctgctg cgaagcgccg gaaaagggta
     6301 gaccggcgct ttgccgtccg gtggtgcgcg aggtaaagcg cgccgctctg cgataatgtt
     6361 ggttccatga tttcgctccg gtaaaagtgc ggcccggcag ggacggactt gcgagaaggt
     6421 cgaccaatct acagcacaaa cgcgcgttac ctaatgttat tcctccgcga acaatctttt
     6481 actttctcga aaaggactgc gctttccccg tttgggagta gcatcagcga gaactgaacc
     6541 cgcgatgcaa aagagagatt cagccaaaac ccaacgcgct cgcctgctgg tcttggttga
     6601 tgaatcgaac gtcggcggtt ccgtgcgcac ggcagggcgc ggtcttgatt ggctgaagct
     6661 gcgtgatttt ctggccgggc cccgcacagg gcgcgacctg atcgagatgg tggtgtatgc
     6721 cgggctgccg ccggcaattc ccgcctggca agaggagcgg gacaagaaga acaagttcgt
     6781 ccagtggctg cgctcgaacg gattcatggt cgtgaccaaa gatgggtcgc cggccgaaga
     6841 aggtcattac aaagcgaacg tagacgtgat gatggcgatc gacgcgatgg agctttccat
     6901 cgcgatgcga cctgatgttg tcattctcgt aacaggcgac gcggatttcg cttatctcgc
     6961 gacacaacta cggcgacacg gaattcgcgt cgaagtcgcg tcggtcgcgg caattctggg
     7021 gcacacactg cgagcggccg cgaacgaagt gatcgacctg gcgccgctct ttcggacgtt
     7081 cgagatgatg aagattgcgg agccaacgcc ggcccgggcc ttaccgccgc cgccgcaaca
     7141 gcagcaacaa cgcagaagac ggccacgctt ccgttctgga cagggaacta cgacgtagaa
     7201 cttgaaccgg agagcggttg aacaggcaac gactcgccgg gaacgacaat gctatggcca
     7261 gctgagatca tatcggccgc gctgcttcgc ccgcggtgcg accgcaacgg cgcagatcgt
     7321 cgtggacccc tttcctggac ttcgaagcgt cgtattggct tgacgtcaat aggacgtcag
     7381 gtcagccgat tgacgagatt gaccgggtag ctcgctgagg ccggccgatg cctttgcaca
     7441 gccgtagaaa tcgcgcgcgt cgttgctccc gagccagtgt tgtctcgggc atccaaagtc
     7501 gaaatggggc gcgttggaac gcccgccgaa tgcttctcat ccgctactat ggcacaattt
     7561 agtcaaatcg cggcacgata tcgctttgag caagcaaaac tcagagcccg gacgtttggc
     7621 acgtgagatt ttgaacaact ctaccacccg caccgtctat gctggcacta tgactaaact
     7681 cattgcgtta gtaacggccg gtgtgtttgc ggcgagcgtc gcgttcgcgg gcgaacacgg
     7741 cgcctgcacc aagcaggccg gcaacaagga gaacatggtg gcatgccagg tgtcgctggc
     7801 caacctcaac ctgaccccgg agcagaagag caaaatggat tcgctgatgg aggagcatca
     7861 taaggaaggc tgcaccgcgg agagcgaggc caagtacatg aaagaggcca agaaaatcct
     7921 gaccaaggag cagtacgcca cgttcaaggc cgagtgtaag aagggcgaaa aggaaaagac
     7981 cgaggcctaa gggcgaacgc gtaaaatcaa tcgattgcgc gccgggaatg tttcccggcg
     8041 cgtttcgttt gtacgagtcc gccgatagcg ggtagcggag cccggaaagc ggaaagagcg
     8101 accagcaaga atcgggttga aagacaaaag ctgaagcgct gaaagtctga aaaatggaaa
     8161 tgagtgcgaa cacgtcgggc gattatgtgc taggcacgca tgaggaggag ctggcgcggc
     8221 tcggtttaca gcaccgcgtc tggcggccgg tggtgctcga gtgctggcag agggccggca
     8281 tcactgttgg tagccgggtg ctcgacgtcg gcgccggtcc cggctatggg actgtcgatc
     8341 ttgccgagat catcgggccg actggtcacg tcgatgccgt tgaacgatct accaatttcg
     8401 tgaacgcgat cagagcgacc tgccgggcac gagcgctcag caacgtgcag gtgcacgagc
     8461 tcgacctgat ggtggaggat ttaccgagtg gcccgtacga tttcacctgg tgccgttggg
     8521 tgctctgttt tgtttccaat ccggatttgc tcgtgcgcag aatcgccgcg gtgacgcgga
     8581 ccggcgggcg ggcgatcttt cacgaatacg ggcattacac aacgtggcgc ttctttccgc
     8641 atcggccggc gttggaagag ttcgttgggc atgtcgtcgc cacgtggcga caatccggtg
     8701 gtgaacccga taccggttta atgttgccgg agtggttgaa cgaaaatgga ttcagcgtgc
     8761 tttcggttga gccgcggatc ttttgtgtgc ggccgaacga ttacatgtgg cagtggccgg
     8821 cgcagttcat cgacgtgcac ctcggccgat tgcaggagct cggccgaatc aataagagct
     8881 ttgccgcaaa agtgcgcgct gaattagccg cagcggaaaa aggtgacgcg tcattcctga
     8941 tcacaccatt ggtgctcgag ataatcgctg agcgagttag aaagtagaaa cgccgagaag
     9001 aagttgatat cggaaagcgg ataccggata ccggaagacg ataggtccgc ggaaaacgct
     9061 caacgttaac gttcaagtta agatgaaaga tctgacgcaa ggatcggtga ccaagcattt
     9121 gctgcacatg tcagcgttca tggcggtgag catgttggcg cagacgttgt atttgctggc
     9181 ggacctgttt tgggtgggac gcttgggaaa ggaagcgatc gccgccgtgg gcgtagcggg
     9241 caacctgatg atgattgtgc tcgcgctgac gcagatgctc ggtgtcggga caacggcgct
     9301 gatctcacaa gcagccggac gaaaagatca gacgcacgcc gaagtcgtgt tcaaccaggc
     9361 gtgcatgatg tcgatcttga tcgcggcagc gcttggcctt ctcgggtttt taacgatgga
     9421 catttacgcc aattcgctca gcgcagacgg gacaacggcg aggctggcaa aggcgtacct
     9481 gtggtggttt ttgcccgcgc tcttgctcca atttcccctc gtcgcgatga gctcggcgct
     9541 gcgcgcgact gggatcgtgc gggcgccagt catgtttcag gtgttgacgg tcgtgcttaa
     9601 catggtgctg gcgccgttcc tgattttcgg aatcggccca tggccaaggc tcggcgtaag
     9661 cggagcggcg ctcgcgacgt tcatctcgat tctcgtcggc aacgtgctca tcatttttta
     9721 tttcgagaaa aaatatcgtt acctgcggtt ccgtttcccg ctttttcgtc cgcaagcaaa
     9781 aatttggagc gcgatgttgc ggatcggcgt gccggccggc gcggagttca tcctgctctt
     9841 tttctatatc gtgctcgttt acgcgatcat ccgcgggttc ggtccggcag cacaagcggg
     9901 ctttggcgtg ggcgcgcggg tgatgcaggc gctgttctta ccggtggtgg cgcttagttt
     9961 cgccgtgtca ccggtggtgg gacaaaactt cggtggacgc cgcgcggatc gggtgcggca
    10021 atcagtttac tccgggctgg gcattgcgac ggtgctgatg gtggtgctgg cgttgatcac
    10081 atgggcggcg ccggaagtga tgatccgctt tttctccaaa gacgcgcggg tgattgcgtt
    10141 cgggagcgat tatctgcgca tcgtttcatt caatttcgtt gcggcgggaa ttgtcttcac
    10201 gagctcgagc atgttccagg gcctcggtaa cacggtgccg ccgttgctga gctcggcgac
    10261 gcggctcatc ctgttcgcgt tgccggccgt gctcatttca cgcgcatcgg gattcgatat
    10321 ccggcacgtc tggtatctgt cggtcggctc gcaagttcta caggcgtgca tcaatctcgt
    10381 gctgctccga cgcgaactgc atcacaagct gcgcttcgca gaaggcgaag ggtttattcc
    10441 ggcgagcgcg accgcgtcgt aaaacaacat gcggttaacg gtttggatta gtctgcggcg
    10501 ccaacgtgga atgcaccagg aatcggaggc ttccgaactt ttgctgtcgc gtgttttttc
    10561 gagctttggt tatgatgcgg tgatgaaact gaaggtgacc gtgttgctga gcttattgtt
    10621 tattttgctg ccgtcagttg ctgtccgcgc gcagacgaaa agttcggcct cagccaaacc
    10681 ggtccgcgaa accgcagcgg agagtgacat ccggaaatgg ctggacgatt ggacaaaggc
    10741 gttcgcgcgg aaagatgtgg acgcggtgat cgcgttgtat gccgatgatg tgattgctta
    10801 cgacgttgtg ccgccgctgc agtacgtcgg gaaagaagcg tatcgcgcgg attatcaaca
    10861 gttcttcggc cagtatcaaa gcgatctgca cgttgaagtt cgcgacctgc acatcggggt
    10921 gcgtggcgat ttcgcctacg ccgccgggct cgagctgatc ggcggcacgt tgaagaacgg
    10981 gcagaaatca gatgtctggg tccggttcac gtcgctcttc cgcaaagtga aaggacgctg
    11041 gctcgatttt cacgatcacg tttcggtgcc ggcggatttc gacactggca aggcgttgct
    11101 cgacctcaag ccataaacat tcgcgcgcac gtctcgaagg tcagggtgtg aaattcgcag
    11161 aaaggcgtgc atgatttgaa actgatcacg tggccgctag ggtgtcgcgt aatgatgcga
    11221 ttggttttta tttgcgctgc gttgtcgatc ttcgggggcg cggcgtttgg cgcgggcgag
    11281 tatcagcgca ccaaggacag gaaaacgctg atctggaatg ctgatccaaa gtcggacgat
    11341 agcgcgagct ggtggggcga gcgcgataag gacggttacg ccaagggcgt gggcgaactg
    11401 acgtggtata cggcgcaagg caccgtttac gcgcgctatt acggcaacat ggtgcacggc
    11461 aaatttgatg gaccggtcaa tgtccacgta aagggaagaa ccgcgcacgc gttcttcagc
    11521 ggaggaaaac ggatgaccag ctgggcgcgc gggcctgcgt cgtcgcgatt cgcgaccgat
    11581 tggcgatcgg cactcgcgag agagaaagct gaggcagctc cggcaacttc gccgccacca
    11641 acaagcaccg agcctgtatc cgcttcggag gagctacggg gcagtgcgca gcagacgcaa
    11701 gaaagcccag ccgaaggacc gacaggagaa gcggcaagta agaaggaaga tttagcaaag
    11761 gcgaagttcg ccccagcgga gctgcccgca cgcggagaag aaggcgcaac gaaagcagaa
    11821 gagaacgttc aacctccaac gcccaacccg
//
LOCUS       DASTDG010000270         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U813
            k141_545983, whole genome shotgun sequence.
ACCESSION   DASTDG010000270 DASTDG010000000
VERSION     DASTDG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968823
            Sequence Read Archive: SRR25158429
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Pseudomonadati; Verrucomicrobiota; Spartobacteria;
            Chthoniobacterales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:40:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,815
            CDSs (total)                      :: 1,787
            Genes (coding)                    :: 1,784
            CDSs (with protein)               :: 1,784
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545983"
                     /isolate="SMAG_U813"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.07444444 N 114.1177778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFO30_09065"
     CDS             complement(<1..930)
                     /locus_tag="VFO30_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9419478.1"
                     /translation="MKALYFRDTGSLANLRVEDIEVPRIDAHGNEALVRVRAAAINPS
                     DPKSVLGKISETTVPRVPGRDFAGIVVEGPATWKGKEIFGTGGDLGFGRNGSHAEFVS
                     VPVEALVEKPVQLSFEQGAALGLGYLTAWSALVTAGRVSPNDIVLVLGATGAVGSAAV
                     KVAKHIGAKRVVGVLRKEADRTRTAGVPSDDWLVLEKEPLPQGLFEITQGKGADVILD
                     AVGGSSFESVNQCLAHRGRHVVIASIEPRVSFNLLDFYHREARLIGVDTLKLSFSESA
                     AVLREIVPLVNAGVLTPPQLETIAIEQGPRAYRA"
     gene            complement(1108..2085)
                     /gene="nrfD"
                     /locus_tag="VFO30_09070"
     CDS             complement(1108..2085)
                     /gene="nrfD"
                     /locus_tag="VFO30_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9419479.1"
                     /translation="MPNEQRLEQLREKAWQEGVVPGRGVDVVGGPIPRKPGYYGQPVV
                     KPPVWTWQVPLYFFFGGIAGMSAVIASGAVIFHHVDLARAAMWLAAIAGAILSPILLI
                     MDLGRPHLFLNMLRVFKHRSAMSMGAWILSAFGACAVPGLIALELHARQIFPGTLDQL
                     LRLAAGIFIFGSAIFGTLLATYTGVLIGATAIPAWFLHRTLLPIHFGTAGLGSAAAVL
                     ELLGHRIPELNFLGFYAAAVETALLIWLSVDKHGAADRAIHEHGSGWLIRIGEVLNGP
                     LAIVFRLGGLIPFAAISFLIGALVSRFGWIAVGKVSGSDPESVFAAERY"
     gene            complement(2272..>2977)
                     /locus_tag="VFO30_09075"
     CDS             complement(2272..>2977)
                     /locus_tag="VFO30_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9419480.1"
                     /translation="FTWSGNSYDNTGHLGASTWRHVMFLEQDRAKGDQVAGPMSIDQD
                     PFRWVFLSDVCKHCEEAGCLEACPTGSIVRTEVGSVLVQNDVCNGCGYCVVSCPFGVI
                     DRRPSPLPDAGGAFKCTFCYDRQKSGLVPACAKVCPTESIVFGRLDDLRARGAERVQQ
                     LQQSGYRDAQLYDPTETSVHGIHAFFLILGEPEAYGLPPKPQIPTIYLKSAWTSAFAT
                     AIASIIVTLMAFAFFS"
BASE COUNT          705 a          880 c          821 g          571 t
ORIGIN      
        1 ggctcggtaa gcacgcggcc cttgctcgat agcaatagtc tctaactgag gcggcgttaa
       61 gacgcccgcg ttcaccagtg ggacgatttc tcgcaggaca gccgcgctct cagagaacga
      121 aagcttcaaa gtgtcaacgc cgatcagtcg tgcttcacga tgataaaagt ccaacaaatt
      181 gaacgaaact ctcggctcga tggatgcgat tactacgtgg cgaccgcgat gagccagaca
      241 ttggttgacc gattcaaagg aggatcctcc cactgcatca agaatgacgt ccgctccctt
      301 gccttgcgtg atttcgaaaa gaccttgagg caagggttcc ttttccagaa caagccaatc
      361 atcggaaggg acgccagcag tcctagtgcg atcggcctct tttctgagaa cgccgacaac
      421 tcttttggcg ccgatatgct tcgcgacctt caccgccgca gatccgaccg cgcccgtcgc
      481 tccgagcacg aggacaatat cattggggga cacgcgaccc gccgtgacaa gcgcgctcca
      541 tgcagtcaga tacccaagac ctaaagcagc accttgttca aaactgagtt gcacgggttt
      601 ctcaaccaac gcctcgaccg gcacagatac aaactctgca tgtgaaccat tccgcccaaa
      661 cccaagatct ccaccggttc caaaaatttc tttgcctttc catgtcgctg gaccttcaac
      721 gacgatgccg gcgaaatccc tccccggcac tcgaggaacc gtcgtctctg aaatctttcc
      781 gagcacgctc tttgggtcgc ttgggttgat tgcggcggcg cgtacccgaa ccagagcttc
      841 attaccatga gcgtcgattc gcgggacttc gatgtcctca acccgcagat tcgcaagcga
      901 tccagtatcg cgaaagtaca gcgccttcat gctggtcgtg aatccgcatg caccatttca
      961 gggcgagact tcgaagtcga atccttctgc acctcttccg ccgtcaatcc gatagttatt
     1021 cacctgtcac gacactagta gccgtcatgc ggcgactgcg tatgcgcact cgcgatgagc
     1081 tgtcccacag caaacttgcg gtggtggcta gtagcgttcc gcggcgaaga cagattctgg
     1141 atcggagccg gaaactttgc cgaccgcaat ccagccgaat cggctgacaa gcgcgccgat
     1201 taagaacgaa atggcggcga atggaataag gccgcccagg cggaacacga ttgcgagcgg
     1261 tccgttcaaa acttcgccaa tccgaataag ccagcccgag ccatgttcgt ggatcgcgcg
     1321 atcagcggcg ccgtgtttgt cgacactgag ccagatcaac aatgctgttt caaccgcagc
     1381 cgcataaaat ccgaggaagt tgagctcggg aattcggtgg ccaagcaatt ccaaaactgc
     1441 cgccgcagat cccaagcctg cggttccgaa atggatcggc agcagtgtgc gatgcaaaaa
     1501 ccacgccgga atcgcggtcg ccccgatcag cacgcctgtg taagtcgcga gcaaagttcc
     1561 gaagatggcc gaaccaaaaa taaagatgcc ggcagcgagg cgcagcagtt gatcgagcgt
     1621 gccagggaaa atctggcgag cgtgcagctc gagcgcgatc aaacctggaa cggcacatgc
     1681 gccgaacgcg ctgagaatcc aggcacccat cgacatggcc gatcgatgct taaaaacgcg
     1741 cagcatgttg aggaagagat gggggcgacc cagatccatg atcaacagaa tgggtgaaag
     1801 aatcgctcca gcgatggccg cgagccacat ggccgcgcgc gcaagatcga catggtgaaa
     1861 gataacggcg ccgcttgcga tcaccgcgga catcccggca attccaccga agaaaaagta
     1921 gagcggaacc tgccaggtcc aaaccggcgg tttgaccacc ggctggccat agtagccggg
     1981 ttttcgcggt attggcccac cgacaacatc gacaccgcga ccggggacaa caccttcctg
     2041 ccacgctttc tcgcgcagtt gttcaagtcg ttgttcgtta ggcatagcgg aggattggag
     2101 agcgcacgcg tctcgcgtgc tggcgatggc gccctcgcca tcgcaaactt ttttcttcca
     2161 gagcattgcg gcggggtcgc cgcaatgggc acgcgagggc gcgcgcgctc cccagagatc
     2221 gtgcttgctc gcgtagctct tcgagccgct tttcagatgg cgaatcagtt gtcatgagaa
     2281 aaacgcaaac gccatcagcg tcacgatgat tgatgcgatc gccgtcgcga atgcggaagt
     2341 ccacgcggat ttgagataga tcgttggaat ctgcggcttc ggcggaagtc cgtaagcctc
     2401 gggttcgccg agaatcaaaa agaatgcgtg aatgccgtgg acggacgttt cggtcggatc
     2461 gtaaagttgc gcgtcgcggt aaccgctttg ttgtaattgc tgaacgcgct cggcgccgcg
     2521 cgcgcgcagg tcatccaatc ggccgaatac aatcgactcg gtcgggcaaa ctttggcgca
     2581 ggccgggacg aggccgctct tttgtcgatc gtaacaaaac gtgcatttaa atgcaccgcc
     2641 cgcatccggc agcggcgatg gtcgtcgatc aattacgccg aagggacacg agacaacgca
     2701 ataaccgcag ccattgcaaa catcgttctg caccaacacc gaaccgactt cagtgcgcac
     2761 gatcgacccg gtagggcacg cttcgagaca accggcttcc tcacaatgtt tgcaaacgtc
     2821 ggacaggaaa acccaacgga acggatcctg gtctatcgac atcggtccgg ccacttgatc
     2881 tcctttcgcg cggtcttgtt ccagaaacat cacgtgccgc cacgtcgatg caccaaggtg
     2941 gccggtgttg tcgtaagaat ttcccgacca tgtgaat
//
